LOCUS       DAOMUJ010000001        23026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_343_length_23026_cov_3.196552, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000001 DAOMUJ010000000
VERSION     DAOMUJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 23026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23026
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_23026_cov_3.196552"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLT73_00005"
     CDS             complement(<1..323)
                     /locus_tag="PLT73_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM18794.1"
                     /translation="MEEFKALWAENAGTKVETMVRTFKKEDLPDGEVLIEVHYSSVNY
                     KDGLAGTNPKSGVIRNYPMILGIDLSGVVVESLDPAFRAGDKVVVTGYGLGVNHFGGF
                     SQYARV"
     gene            complement(462..1283)
                     /gene="jag"
                     /locus_tag="PLT73_00010"
     CDS             complement(462..1283)
                     /gene="jag"
                     /locus_tag="PLT73_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRM18795.1"
                     /translation="MDKVIAKDTTVDKAIRKGLDMLGVDSHEADIVIISEGKKGIFGF
                     GQKEAVVEITRKEIRDFDEAGDIQLAEIEETVEDIVEDLEEDYPEAAEKILADQPEVD
                     KVIYSDENVESFPEDYDEESGRDEAIAAVTAYLTSVAEAYGAPAIVTVEEKNNMLIFQ
                     MESEKPGLLIGKYGKIVNALQILAQALVQRYVRRRLSVIVNVGDYRERRAAVLESIAD
                     RTAERVLKTRQPVFLEPLPAFERKQIHARLSQNKRIKTHSEGKEPHRYLVVEIAD"
     gene            complement(1319..2149)
                     /gene="yidC"
                     /locus_tag="PLT73_00015"
     CDS             complement(1319..2149)
                     /gene="yidC"
                     /locus_tag="PLT73_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRM18796.1"
                     /translation="MSKRKRLLFTLSLFAVMMVLSACGTEPITSESTGFWDRYVVLNF
                     SNMIVWLSNLFFSNYGLGIIAFTIIIRVILLPLNHFQTKSTKKMSAVQPKVKALQEKY
                     ASKDRETQERLQKEVSKLYADNDVNPYMGCLPVLVQMPVLMALYQAISRTEILKSGSF
                     LWMNLGERDPFFILPVVAAILTYATSKLTMMSQAEANSATKSMTYTMPIMILMMGINF
                     PSALSLYWVASNAFSVGQTMLLNNPYKAIREREEAEAQAKAREKALKKAQNPKKKKKN
                     "
     gene            complement(2181..2525)
                     /gene="rnpA"
                     /locus_tag="PLT73_00020"
     CDS             complement(2181..2525)
                     /gene="rnpA"
                     /locus_tag="PLT73_00020"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRM18797.1"
                     /translation="MRKSFRVKKEKEFQKVFHNGVSKANRQFVIYVLDKKGQPHFRVG
                     LSVGKKIGNAVVRNAVKRKIRQALTELKEELVQDIDFIVIARKPVAEMSAAEVKKSLI
                     HVLKLSNLLKKK"
     gene            complement(2751..2885)
                     /gene="rpmH"
                     /locus_tag="PLT73_00025"
     CDS             complement(2751..2885)
                     /gene="rpmH"
                     /locus_tag="PLT73_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRM18798.1"
                     /translation="MKRTYQPKKRHRQSVHGFRKRMSTKNGRRVLARRRAKGRKVISA
                     "
     gene            3456..4787
                     /gene="dnaA"
                     /locus_tag="PLT73_00030"
     CDS             3456..4787
                     /gene="dnaA"
                     /locus_tag="PLT73_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRM18799.1"
                     /translation="MDDIYSLWRYLQEKFQESLSKVSYDTWIDSAVPVRLTDTNIIIE
                     VPSSLHKDYWQNNLMSRIVEGIYEFSGKELTPIFMIKNEEMPDIAKPTIKRTNNNRLF
                     KDAQLNSKYTFDTFVIGKGNQMAHAAALVVAEEPGTIYNPLFFYGGVGLGKTHLMHAI
                     GHQMLQLRPTARVKYVSSETFANDFINSIQNRTQEQFRNEYRSVDLLMVDDIQFFADK
                     EGTQEEFFHTFNALYDERKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLET
                     RIAILRKKANSERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAYSAIQGTDITTSLA
                     ADALKSIMPSEKPKALSIYAIQQAVGKYYHVTVEELKGKKRTKGIVVPRQIAMYLSRV
                     MTDNSLPKIGSEFGGKDHTTVIHAHDKIALSVKKDPVLQREIETIQNSLKK"
     gene            4965..6116
                     /gene="dnaN"
                     /locus_tag="PLT73_00035"
     CDS             4965..6116
                     /gene="dnaN"
                     /locus_tag="PLT73_00035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRM18800.1"
                     /translation="MKFSINRQVLIKHLSDVQRAISSRTTIPILTGVKISADENGIVL
                     SGSDSDISIETLIPVSEENNQIEIHTQGGIVLPARFFSEIVKKLADEKITIEVKDHFQ
                     TNITSAKASFTINGIDVNNYPNFPVIDSNEVITLPTALFKQVIQHTVIATSTQESRPI
                     LTGVNMTIKDGKLTAVATDSHRLSQRIISIAAPESQLEKNYNVIIPGKSLVELSRIVE
                     NQPTIEMMITENQVLFKAENVYFYSRLLEGYYPDTNRLIPASSSTQIVLNAYDLLQAT
                     DRASLLSHEGKNNVVKLSIDSNKVELSGNSPEVGNVEESLAYQSASGDPLIISFNPDY
                     MKDALRTFGQQDVQVNFTSAVRPFTVVPADREDENDNSFIQLITPVRTY"
     gene            6480..6746
                     /gene="yaaA"
                     /locus_tag="PLT73_00040"
     CDS             6480..6746
                     /gene="yaaA"
                     /locus_tag="PLT73_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HRM18801.1"
                     /translation="MKNIVNIDAEFITLGQLLKHVNLISSGGMAKWFLSEYVVYVDNE
                     KEDRRGRKLFPGTMIDIPGEGTFFIQSTKSDKEEAEQDNSESEA"
     gene            6743..7864
                     /gene="recF"
                     /locus_tag="PLT73_00045"
     CDS             6743..7864
                     /gene="recF"
                     /locus_tag="PLT73_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRM18802.1"
                     /translation="MILKEVTLQNFRNYPSLSVSFSDGINVFLGENAQGKTNLMEAIY
                     ALALARSPRTSNEKEMIRWQEDSAKITGKIQKRVSTFPLEIIFSKKGKIAKVNHLEQK
                     KLSQYIGQLNVVLFAPEDLNLVKGAPANRRKFLDMELGQMNPVYLHDLVQYQRILKQR
                     NLYLKQLVTGKAKDEVYLDVLTEQLAILGADLLVYRLQFIKKLEAWAQPLHKEISLER
                     EELTIAYKSNVELSDEMDKDALFLKLMEAFKQGKSRERDQAVTLFGPHRDDLVFQVNG
                     RNVQNFGSQGQQRTTVLSLKLAEIECMNEVLGEYPILLLDDVLSELDDERQTHLLKAI
                     EKKVQTFLTTTSLDGIKKNKIDEPTIYHIKNGEVEMEGV"
     gene            7869..9806
                     /gene="gyrB"
                     /locus_tag="PLT73_00050"
     CDS             7869..9806
                     /gene="gyrB"
                     /locus_tag="PLT73_00050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRM18803.1"
                     /translation="MVENEKNKAERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSGAG
                     LHHLVWEIVDNSIDEALAGFATKIEIAIEEDNSITVTDNGRGIPVDIQANTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSSKLSVSVHKDNKIYTQTFERGDIT
                     SELTVTGDTDKHGTTVNFLPDPEIFRETTVFDYEKLAVRVRELAFLNKGLNISIVDNR
                     PEEPLKQEYHYEGGIKSYVEFLNRNKKVLFEEPIYIEGEQDDIQVEVGIQYTDGYHTN
                     FLSFANNIHTYEGGTHESGAKTALTRVINDYAKRNKIIKENEENLTGEDVREGLTLVL
                     SIKHPDPQFEGQTKTKLGNSEARTITDRLFSTYFDKFLMENPQVARQIVEKGMLAARA
                     RLAAKRAREVTRKKSGLEISNLPGKLADCSSKNPAESELFIVEGDSAGGSAKQGRSRF
                     FQAILPIRGKILNVEKASIDKILANEEIRSLFTAMGTGFGGDFDVSKARYHKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPLVEAGYVYIAQPPLYQVKQGKKEKYLDTDQELEEYL
                     ASIPDSPKPSIQRYKGLGEMDAEQLWDTTMNPENRHFLQVTVDDAVKADETLNMLMGD
                     HVEPRRNFIEENAVYVKNLDI"
     gene            9861..12455
                     /gene="gyrA"
                     /locus_tag="PLT73_00055"
     CDS             9861..12455
                     /gene="gyrA"
                     /locus_tag="PLT73_00055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM18804.1"
                     /translation="MEQRELSHEMETSFLEYAMSVIVARALPDVRDGLKPVHRRILYG
                     MSELGVTPDKAHKKSARIVGDVMGKYHPHGDSAIYESMVRMSQDFSYRYPLVDGHGNF
                     GSIDGDSAAAMRYTEARMSKIALEMLRDLNKDTVDYHDNYDGSESEPDVLPARFPNLL
                     VNGASGIAVGMATNIPPHNLTEVISALHILMNNPDATTAELMEAVPGPDFPTGGIVMG
                     KSGIRKAYETGKGSIIVRGRVEVEILKNGKERIVISELPYMVNKAKLVERIAELARDK
                     RIEGITDLADESDRDGMRVVIDVRKDVSASVILNNLYKLTPLQSSFGFNMLAIVKGIP
                     KVLGLKQILVHYLEHQEEVIRRRAAFDKRKAEARAHILAGLRIALDHIDEIVAILRGS
                     NNGDAAKQIFIDKYGLSEKQAQAILDMRLVRLTGLEREKVEAEYDALMALIADLADIL
                     ANEQRVFDIIYTELLEIQERFGDKRRTELLVGEVLSLEDEDLIEEEDIVLTLTHNGYI
                     KRLPNSEFRAQKRGGRGVQGMGIHDDDFIETLISASTHDVLLYFSNQGKVYKTKGYEV
                     PEYGRQAKGLPIINLLNLDANEKIQAIINVKGGPQDGDYLFFTTRMGTVKRTSATEFQ
                     NIRQSGLRAINLKDEDELIKVSLTDGNQNIIIGTHFGYSVSFDEKDVRDMGRTATGVR
                     GVKLRENDYVVGMDVLKHESEVLIITENGYGKRTAADEYAIKGRGGKGVKTANITEKN
                     GKLVGLTTVSGEEDIMIITDKGVMIRFHADAISQTGRATLGVRLIKLDGEAIVSTMAK
                     VEREEDEEVVAAPLNEEIDSEGENDALEAFDATEQANTLDTTETSEMADKLSDFADEL
                     LDEEDSEE"
     gene            12922..13215
                     /gene="rpsF"
                     /locus_tag="PLT73_00060"
     CDS             12922..13215
                     /gene="rpsF"
                     /locus_tag="PLT73_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRM18805.1"
                     /translation="MNNYEINYIIRPNIEEEAKAELIARFDSILTDNGAEVIESKDWA
                     KRRLAYEIKDFREGIYHIVKISASDAKAIDEFDRLAKINSDILRHIIVKEEAK"
     gene            13254..13805
                     /gene="ssb"
                     /locus_tag="PLT73_00065"
     CDS             13254..13805
                     /gene="ssb"
                     /locus_tag="PLT73_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM18806.1"
                     /translation="MINNVVLVGRLTKDVDLRYTSSGTAVGTFALAVNRQFTNQAGER
                     EADFINCVMWRKSAENFANFTRKGSLVGITGRIQTRNYDNQQGQRVYVTEVVADNFTL
                     LESKTTTEQRPRETGAGSSNQGAPSANFGSSKSFESNQNQGQYNNQYSSPKQNGFSDF
                     NSTADPFSSNGESIDISDDDLPF"
     gene            13849..14091
                     /gene="rpsR"
                     /locus_tag="PLT73_00070"
     CDS             13849..14091
                     /gene="rpsR"
                     /locus_tag="PLT73_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRM18807.1"
                     /translation="MAFQRNSRGGRKRKKVCYFCANHIDHVDYKETDLLKRYISEKGK
                     ILPRRVTGTCAKHQRTLTVSIKRSRIMALMPFTLAE"
     gene            17326..19023
                     /locus_tag="PLT73_00075"
     CDS             17326..19023
                     /locus_tag="PLT73_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRM18808.1"
                     /translation="MNPYLIKYFGKKDTLGRKLNVVDEELYQLFKGSQDYVMRRVKWG
                     DHYFDMIIQEDIGVIYLMDITEYAKIEERYKDEKFVFGNIIVDNYDEAVQSMNDRKRS
                     NVNNYLTNQLTNWANMNNVFLKRVDDDRFIALTNMRALKKIEENNFDIINQIRERTYK
                     QNFPLTLSMGFSYEKTFEQTNFKEVAKMAQSNIDLALARGGDQVVVRAEDEDARFYGG
                     KTNPMEKRTRIRARMISQALEELIVHADKVIIMGHSYPDMDVIGSCLGIRRISQMNNR
                     EAWIVLDPQQFSNDVNRIMQEIDKDPLIAKRIISPEQAMALVTPQTLIVMVDVHRPSM
                     TPAPGLLNLSKKIVVLDHHRRGQEFPKDSVLVYMEPYASSTSELIAELFEYQSNEMDA
                     INKIEATAMLAGIIVDTRNFSLRTGSRTFDAASYLQSNGADTVLIQKSLKEDLDTYLL
                     RSHLLQTMEFVNGNLAIVHGEDEKIYDTVVAAQTADTMLSMEGVDASFVVTKRQDGRV
                     GISARSLGQYNVQTIMEKLGGGGHLSNAATQIENTTVHEVVERLKAVIDPKKIKEGE"
     gene            19027..19476
                     /gene="rplI"
                     /locus_tag="PLT73_00080"
     CDS             19027..19476
                     /gene="rplI"
                     /locus_tag="PLT73_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRM18809.1"
                     /translation="MKVVFLEDVKGKGKKGEVKDIADGYANFLIKNRKAEEANAATIS
                     ALKGQKKAEEKKQAEELAAAQELKALLEKDESIIKLSAKIGEDGRLFGSITTKQVAEA
                     LQKQHNVKLDKRKIELDSPIRALGFTKMPVKIHPEVTAMLTVQVTEG"
     gene            19691..21058
                     /gene="dnaB"
                     /locus_tag="PLT73_00085"
     CDS             19691..21058
                     /gene="dnaB"
                     /locus_tag="PLT73_00085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRM18810.1"
                     /translation="MEETLQDRIPPHSIEAEQSVLGSIFLDPETVVNVLEYLETSDFY
                     RKNHQIIFDAILQLNNRNEAIDVVTIANELDTKNQLENAGGMEYLAELAVAVPTSANV
                     EYYAKIVEEKSILRNLIRSATEIVRKGYEEGDELAVMLDSAEQNILQVSERRNRSGFI
                     RISDVVSASLQNIESLAQQSDDVTGVPTGYIALDKMTAGLQKEELIILAARPAVGKTA
                     FALNIAQNVATKADQVVAIFSLEMGAESLVNRMLCAEGNIDAGHLRTGQLSEDEWSNL
                     IMAMGTLGQSKIFIDDTPGIRIAEIRAKSRRLLQEQGKLGLIVIDYLQLIEGNNRESR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGEEPERDEQENNVVEVIIEKNRSGARGTVKLLFKKEFNKFSSMSY
                     MPEIS"
     gene            21178..22473
                     /locus_tag="PLT73_00090"
     CDS             21178..22473
                     /locus_tag="PLT73_00090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRM18811.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIQ
                     FDGVTYKLHLIPSGIFYPEKISVIGNGVVVNPKSLIKELAYLKDHGINTDNLRISDRA
                     HVILPYHIQLDQLQEDSKGENKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERL
                     KINLEEKNRQFTKMFDSVEIKFEDIFEEYYQYGQEIKKYVCDTSVILNDVLDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPTKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGNQIREVGREYGTTTGRPRRIGWFDAVVMRHSKRVSGITNLCLNSIDVL
                     SGLETVKICTAYKNAEGELIYHYPASLKELAQCTPVYEELPGWSEDITGCRTLEELPE
                     NARNYVRRVQETVGVRISTFSVGPDRNQTNVLENVWASI"
     gene            22607..22679
                     /locus_tag="PLT73_00095"
     tRNA            22607..22679
                     /locus_tag="PLT73_00095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22640..22642,aa:Lys,seq:ctt)
     gene            complement(22747..>23026)
                     /locus_tag="PLT73_00100"
     CDS             complement(22747..>23026)
                     /locus_tag="PLT73_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18812.1"
                     /translation="AIFIGVLSMLSGSRLPAIILLLLKLSNAASLVNNGTEILLITFL
                     ATVTPAASTVTQFAQVHDKNAAYAGAINIMTTLLCIVTMPIFVILYYI"
BASE COUNT         7062 a         4838 c         5169 g         5957 t
ORIGIN      
        1 actcgggcat attggctgaa tccgccgaaa tgattcaccc ctagaccata gccagtcacg
       61 accaccttat cccctgcccg gaaagccgga tcgagcgatt ccacgaccac gccgctcaga
      121 tcgatcccca aaatcatggg ataatttcgg atgaccccgc ttttcggatt ggttccggcc
      181 aggccgtcct tgtagttgac gctcgaatag tgcacttcga tcagcacctc cccatccggc
      241 aagtcctctt tcttgaacgt tctgaccatt gtttccactt tcgtgcctgc gttttccgcc
      301 cataaagcct tgaattcttc catactaccc ctcgtttcag cttattttat ctatagttta
      361 gtccatcttc ccagaatttg cgacttcttt aaaaagagcc aaataaaaaa accttcaccg
      421 agaatcaagc tgatccctga tgaaggtttt aggatattcg attagtccgc tatctcaaca
      481 accaaataac gatgcggctc tttgccttcg gaatgggttt tgatgcgttt gttctggctc
      541 aagcgcgcat ggatctgttt tctttcgaaa gcaggcagcg gctcaaggaa cacgggctgt
      601 ctcgttttga ggacgcgttc tgcagtacgg tcagcgatgc tttccaaaac ggcagctctg
      661 cgctcacggt agtcaccgac gttcacgatt acggacaagc ggcgacgcac ataacgttgc
      721 accagcgctt gggccaatat ctgcaaagca ttgacgattt ttccgtattt gccgatcaat
      781 aagcctggct tttcgctttc catttggaaa atcagcatgt tgttcttctc ttcgaccgtc
      841 acgatcgcag gagccccgta tgcttcagct acagatgtaa gataagcagt cacggcggca
      901 atcgcttcat cgcgaccgga ttcttcatca taatcttccg ggaaagattc cacgttctcg
      961 tccgaataaa tcaccttatc gacttcaggt tgatcagcca atatcttttc cgcagcttcc
     1021 ggataatcct cttcaagatc ttctacgata tcttcgactg tctcctcaat ttcggctaat
     1081 tggatatcgc cggcttcatc aaagtccctg atttctttgc gcgtgatttc cacgacagcc
     1141 tctttttgtc cgaatccgaa aatccctttt tttccttcag agattatgac gatatcggcc
     1201 tcatggctat cgacgcccaa catatccaga cctttgcgga tggctttgtc aacagtcgta
     1261 tctttcgcaa ttactttatc cataatagaa tgtcatcctt tcctacttca aagtctaact
     1321 agtttttctt tttcttttta ggattttggg ctttcttcaa tgctttttcg cgcgcctttg
     1381 cctgagcttc agcttcttcg cgttcacgga tggccttata cggattgttc aacaacattg
     1441 tctgtcctac cgagaaggca ttgctggcta cccagtacaa cgacagggca ctcgggaagt
     1501 tgatacccat catcaaaatc ataatcggca tcgtgtaagt catcgatttt gtcgcagaat
     1561 tcgcttctgc ttgtgacatc atcgtcagct tactggtggc atacgtcagt atcgcggcca
     1621 ctaccggtaa aataaagaat ggatcacgtt ctcccaagtt catccagagg aagctaccgc
     1681 tcttcaaaat ttcagtccgg ctgattgctt gatacagtgc catcaataca ggcatctgaa
     1741 ccaaaacagg caaacatccc atataaggat tcacatcatt atctgcatac agcttagaaa
     1801 cttccttttg cagacgttcc tgcgtctccc gatctttaga ggcatatttt tcctgcaacg
     1861 cttttacctt tggttgaaca gccgacatct ttttcgtgct cttcgtctga aaatgattga
     1921 gcggcaacag gatgacccgg atgatgattg tgaaggcaat gattcctagg ccataattac
     1981 tgaaaaagag attggacaac cacacgatca tgtttgagaa gtttaaaaca acataccgat
     2041 cccaaaaacc ggtactttct gaggtgatag gttccgttcc acatgctgat agaaccatca
     2101 taacagcaaa taagctgagt gtaaaaagta atcgcttacg ctttgacaag agaactcctc
     2161 cttaatagga ataatatagt ttattttttt ttcaaaaggt tggaaagctt aagaacatgg
     2221 atcaggcttt tctttacttc cgcagccgac atttcagcaa ccggcttgcg tgcgataacg
     2281 atgaagtcaa tatcctgaac caactcttcc ttcaattccg tgagtgcctg gcggattttt
     2341 cttttgacgg cattccgcac tacggcgttg ccgatttttt tgccgacgga caaccctacc
     2401 cggaaatgcg gttgtccttt tttatcgagc acataaatca cgaattggcg attcgctttg
     2461 gaaacaccat tatgaaatac tttttgaaat tctttctctt tcttaacacg aaaagatttc
     2521 ctcatgtttc ttccctcaaa tctgcaatca taacatacaa aaaaatctcc cgaaaatgga
     2581 gaaaaacaga aaaatttctc tctccgttat catcacaaat gcaaacccac tcccgaagct
     2641 ttacagcttt cggttctgtc ggacacattt gtcacactct ccatttaaga aagacatttt
     2701 tgcatttttc gaaatactta aaaaaaaaga ccactgaaac ggtcagtgat ctatgcagag
     2761 ataacttttc tgcctttagc gcgtcttctt gctaatactc tacggccgtt ttttgtgctc
     2821 atacgtttac ggaaaccatg aacggattga cggtgacgtt ttttaggttg gtatgttctt
     2881 ttcattgatc tttacacctc ctgataaact atctttcaaa taggttatat ttcactgttt
     2941 agacagtcca atacattata aagagacaga atgtgacctg tcaagtaaaa ttggttcatt
     3001 tttattgaac tcactgagat ataatagcac aaaacaatga aagattaaac acctttcttg
     3061 aaaaaaattt gattttcatc gaaataaaat tatccacaac ctttttttta atgcacaatg
     3121 cccatggata actcctgcaa tcccctcgct tgcacacacc ttcgaaccga cttgtccaca
     3181 ttcttacagg gtgtgtattt ccgtaggata accacttccg gaacttgtgg ataagtgttc
     3241 caaaagctgt aacaatggta tttaaacccg aattaaaaca cacaaaaaac cgtggataac
     3301 aacttcgtcc acaaaccttg tccacatcct aaatcgacct gtggacaact atttatacac
     3361 cggttaattg ttttattcac agtttaaaag agttgtggaa aactttttga tataatgcta
     3421 aaataaaaag gactcactaa aaggaggtgt cccatttgga tgacatctat tcattatggc
     3481 gttatctgca agaaaaattt caagaatcac tgtcaaaagt cagctacgat acttggatag
     3541 acagcgcagt cccggtccga ctgactgata cgaatatcat catcgaagtt ccctcttctt
     3601 tacataaaga ttattggcaa aataatttga tgagcagaat tgttgaaggc atctacgaat
     3661 tcagcggcaa agaactgact cccattttta tgatcaagaa cgaggaaatg cccgatattg
     3721 caaaaccgac catcaaacgc accaacaaca atcgtctctt caaggatgcc caactgaaca
     3781 gcaagtatac ctttgacacc tttgttatcg gaaaaggaaa ccagatggct cacgcagctg
     3841 ccctcgtcgt ggcagaagaa cccggaacaa tctacaaccc actcttcttc tatggaggcg
     3901 tcggcttagg caagacccat ttgatgcacg ccatcggcca tcaaatgctg caactgcgcc
     3961 caaccgcgag agtaaaatat gtcagcagcg aaacgttcgc caatgatttc atcaattcca
     4021 tccagaatcg gacgcaggag caattccgca atgaataccg cagcgtcgac ttgttgatgg
     4081 tcgatgatat tcaatttttt gccgacaaag aaggaacgca ggaagagttc ttccatactt
     4141 tcaatgcgct ttacgatgaa cgcaaacaga ttgtactgac gagcgatcgt ctgccgaacg
     4201 aaatccctaa actgcaggaa cggctcgtct cgcgttttgc ttggggctta tcggttgata
     4261 tcacgccgcc ggatctggag acgcgcattg ctattctgcg caaaaaagcc aacagcgaac
     4321 gcctggagat tccggatgac actctcagtt acatcgctgg acagatagat tccaacatcc
     4381 gggaattgga aggtgcactg gtccgcgttc aagcctattc tgctatccaa gggaccgaca
     4441 tcaccaccag cctggctgcg gatgcgctga agagcatcat gccttccgaa aaaccaaaag
     4501 cattatccat ctatgccatt caacaagcgg tagggaaata ttaccatgtg accgtcgagg
     4561 aattgaaagg caaaaagcgc actaagggaa tcgtggtgcc aagacaaatc gccatgtatc
     4621 tttcccgggt catgacggac aattccttgc cgaaaatcgg ttctgaattc ggcggcaagg
     4681 accacacaac cgtcatccac gcccatgaca agattgccct ttccgtaaaa aaagaccctg
     4741 ttctgcaacg cgaaattgag acgatccaaa atagtctgaa aaaatgatcc tctcgcgcat
     4801 tgtggataag ccaataacta agacgccatt tacgaacacg ttatccacaa gtggaaaacg
     4861 attggtttta tcggcttctt tcacttatcc acagtatgaa caggccctac tactattact
     4921 aatattattt aatttatatg aataaccatc ggaggtccct tattatgaaa ttttccatta
     4981 atcgtcaggt tttgatcaaa cacttatctg acgtacaaag agctatttcc agcagaacga
     5041 caattcctat tttgacaggg gtaaaaatca gtgctgatga aaatgggatc gtattgagcg
     5101 gaagcgattc ggatatttcc atcgaaacgc tgattcctgt gtcggaagag aataaccaga
     5161 tcgaaatcca cacacaaggc ggcattgtct tgccagctcg tttcttcagt gaaatcgtaa
     5221 aaaaattagc tgatgaaaaa attacgattg aagtcaaaga tcacttccag acaaacatca
     5281 cttcggcaaa agcttctttt accatcaatg gaatcgatgt aaacaactat ccaaattttc
     5341 cggtcatcga ttcgaatgaa gtcatcacct tgccgacagc cctgttcaaa caagtgatcc
     5401 aacatacggt aatcgcaacg tcgacacaag aaagccgtcc gattttgacg ggagtgaaca
     5461 tgaccatcaa agatggcaaa ttgacggctg tcgctacgga cagccatcga ttaagccaac
     5521 ggatcatctc gattgccgcg ccggaatcac agcttgaaaa aaactacaat gtcatcattc
     5581 caggaaaaag cctggtagag ctgtcacgga tcgttgagaa ccaaccaacc atcgaaatga
     5641 tgatcacgga gaaccaggtc cttttcaaag ccgaaaatgt ttatttctat tcacgtttgt
     5701 tggagggcta ttatcccgat acaaatcgct tgattccagc cagctcaagt acacagattg
     5761 tcctgaacgc ttacgatttg ctgcaggcta ccgaccgtgc ctccttgctt tcgcatgaag
     5821 gaaagaacaa cgtcgtcaaa ttgtccattg actccaataa agtcgagctt tcaggaaact
     5881 cacctgaagt cggtaatgtg gaggaatccc tagcctatca gtccgccagt ggcgacccac
     5941 tgatcatatc cttcaatcct gattacatga aggatgcgct gcgcacattc ggccaacagg
     6001 atgtccaagt caatttcact tcagctgtac ggccattcac agtcgtaccg gcggatcgtg
     6061 aagacgaaaa cgataattcg ttcatccagt tgatcacgcc tgtaaggact tattgatccc
     6121 accgcttaaa tccaaaaagc aaggaaacgt atgctttcgt ttccttgctt tttgtttatc
     6181 tgaaaatgaa aaattttgga tctgctgttt tcctatagga agaaagttag cattcggtcc
     6241 ttttgttcag ataaagataa aatgaataaa aaacgcgaat ttttaattta taagcctata
     6301 gacgctttcg tttggatata gccaatttgt attgttttct aaaaaaattg aaataagggc
     6361 cttttttttc gcgaaaattt gaaattgatc aaaaaaaata gtataataaa cgtattaatc
     6421 agcatggtcg cgtggatttt gttttccacg cttattttat tggaaaatag gtgaatttct
     6481 tgaaaaatat tgtgaatatt gacgcagagt tcattactct tggtcaacta ctgaaacatg
     6541 tcaatctgat ttccagcggc ggtatggcca aatggttcct ttcagaatat gtggtgtatg
     6601 tagacaacga aaaagaagac cgcagaggcc gcaaattatt tcctggtacg atgatcgaca
     6661 tccctggtga aggcactttc ttcattcagt caacaaaatc ggataaagaa gaggctgagc
     6721 aagacaacag tgagtccgaa gcatgatcct gaaagaagtg acgctgcaaa attttcggaa
     6781 ttatccgtct ctttctgtgt ccttttcaga tggcatcaat gtgtttttag gtgagaatgc
     6841 gcaaggtaaa accaatctca tggaagctat ctatgcattg gctttggcaa gaagtccgcg
     6901 tacgtcaaac gaaaaagaaa tgattcgatg gcaagaagac tccgccaaga taaccggaaa
     6961 aatccaaaaa agggtttcaa cattcccttt agaaatcatt ttttccaaaa agggtaagat
     7021 cgctaaagtc aatcatttgg agcaaaaaaa gctgagccaa tacatcggcc aactgaatgt
     7081 tgtgttgttt gctcccgaag acttgaattt agtcaagggt gcaccggcca accggagaaa
     7141 attcctggac atggagctgg gccagatgaa cccggtctac ctgcatgatc ttgtgcaata
     7201 tcagcggata ctgaagcaac ggaatcttta tctgaagcag ttggtgaccg gcaaagccaa
     7261 ggatgaagtc tatttggatg tgctgactga gcaattggcc attttgggtg ccgatctgct
     7321 ggtctaccgt ctgcagttca tcaaaaaatt ggaagcctgg gcccaaccgc ttcataaaga
     7381 gatttctttg gagcgggaag aactgacgat cgcctacaaa tccaatgtcg agttgtccga
     7441 tgagatggat aaggacgctc tttttttgaa actgatggaa gctttcaagc aagggaaaag
     7501 ccgtgaaaga gaccaggcag tcaccctttt cggcccgcac cgtgatgatc tggtttttca
     7561 ggtgaacggg cgcaatgtcc aaaactttgg ctctcaagga cagcaaagaa caactgtcct
     7621 cagtctcaaa ctggcggaaa tcgaatgtat gaatgaggtt ctgggggagt atccgatcct
     7681 cttgttggat gatgtcctgt cagaattgga tgatgaacgc cagacccatt tgttgaaagc
     7741 gatcgagaag aaggttcaga cctttttgac gacaaccagt ttggatggca taaagaaaaa
     7801 taagatagac gaaccgacta tctaccatat aaaaaacggt gaagtagaaa tggaaggtgt
     7861 gtaacagaat ggtagaaaac gaaaagaaca aagctgaacg agctaaggaa tatgatgcca
     7921 gtcagatcca ggttctggaa ggactggaag cagtccgtaa acgtcccggg atgtacattg
     7981 gttccaccag tggcgcaggt ctgcatcatt tagtctggga aatcgtggat aattccatcg
     8041 atgaagcgct ggctggtttt gcgacaaaaa tcgaaatcgc aatcgaagag gacaacagca
     8101 tcactgtaac cgataacgga cgcggcattc ctgtcgatat ccaagccaat accggtcgtc
     8161 ccgctgtgga aacagtcttc actgttcttc acgcaggcgg taaattcggc ggcggaggct
     8221 ataaagtatc cggcggcctg cacggtgttg gagcttccgt cgtaaacgcc ctttcctcca
     8281 agctgtccgt cagtgtacat aaagacaaca aaatttatac gcagacattt gagcgcgggg
     8341 acatcacttc cgaattgaca gtgaccggcg atacggataa gcacggaacg accgtaaatt
     8401 tccttccgga tccggaaatc ttcagggaaa cgactgtttt tgattatgaa aaattagctg
     8461 tgcgtgtgag ggaattggcc tttttgaata aaggactgaa catctcgatc gtcgacaatc
     8521 gtccggaaga acctttgaaa caagaatatc attacgaagg tgggatcaag agctacgtcg
     8581 aattcctgaa ccggaacaaa aaagtccttt tcgaagaacc gatttatatc gaaggcgaac
     8641 aggacgatat tcaagttgaa gtaggaatcc aatatacgga tggctatcat acaaactttc
     8701 tgagcttcgc caacaatatc catacttatg aaggcggaac ccacgaatcc ggcgccaaaa
     8761 ccgctttgac gcgtgtcatc aatgactatg caaaacgtaa taagatcatt aaggaaaatg
     8821 aagagaacct gactggtgaa gatgtgcgcg aaggccttac ccttgttctc tccatcaaac
     8881 atccggatcc gcaattcgaa ggacaaacca agacaaaatt gggcaattcc gaagcgcgta
     8941 ccatcactga ccgacttttt tcaacgtact tcgacaaatt cctgatggaa aacccacaag
     9001 tcgcccgtca gattgtcgaa aaaggcatgt tggccgcccg agcacgcttg gcagccaaaa
     9061 gagcccgtga agtgacccgc aagaagagcg gattggaaat cagcaatctg cccggaaaat
     9121 tagccgattg ctccagcaaa aacccggcag aatctgaatt gttcatcgtc gaaggggact
     9181 cagccggagg ttcggcaaaa caaggacgct cgcgtttctt ccaggcgatt ctgccgatcc
     9241 gcggtaagat tctgaacgta gaaaaagcat cgatcgataa aatccttgcc aacgaagaga
     9301 tccgttcgct gtttacggcg atgggcacag gattcggcgg agacttcgac gtttccaaag
     9361 cgcgttatca taaattagtc atcatgaccg atgccgatgt cgatggcgcg catatccgta
     9421 cgttgctgtt gacgctgttc tatcgctaca tgcgtccttt ggttgaagct ggatatgtgt
     9481 atattgcaca accgcctttg tatcaagtga agcaaggcaa aaaagagaaa tatctcgata
     9541 ctgatcagga actggaagag tacttggcct ccatccctga ctcgccaaaa ccaagcatcc
     9601 aacgttataa agggcttgga gaaatggatg cagaacaatt gtgggatacg accatgaacc
     9661 ctgagaaccg tcacttcctg caggtgactg tcgatgatgc cgtcaaagct gacgaaacat
     9721 tgaacatgct gatgggggat catgtagaac caagaaggaa cttcatcgaa gaaaatgcgg
     9781 tttacgtgaa gaacttggat atctaaaagg gggttggaga ctaaatgtct gatgaaattg
     9841 aaaaagtaaa tgatcaagcc atggaacaaa gggaattgag ccatgaaatg gaaacgtcat
     9901 tcctggaata tgccatgagc gttatcgtag ccagggcttt gcctgacgtc cgtgacggac
     9961 tcaagccggt ccatcgccgt atcctttacg gcatgagcga attgggcgtc acgcctgata
    10021 aagcgcacaa aaaatccgca cgtatcgtcg gggacgtaat gggtaagtac catccccacg
    10081 gtgacagtgc catctatgaa tcgatggtgc gtatgtccca ggatttcagt taccgttatc
    10141 cgctggtaga tggacacgga aacttcggct ccatcgatgg agacagcgcc gcagcgatgc
    10201 gttacaccga agcgcggatg tcaaaaattg ctttggaaat gctgcgtgat ctcaataaag
    10261 atacagtcga ttatcacgat aactatgacg gttccgaaag tgaacccgat gtattaccgg
    10321 cccgcttccc taacctgctg gtgaatggtg cttccggtat cgccgttggg atggcgacaa
    10381 atatcccgcc gcataatctg acggaagtca tttccgcctt gcatatcctg atgaacaatc
    10441 cagatgcgac gactgccgaa ttgatggaag ccgttcctgg gcccgatttc cctactggcg
    10501 gtatcgtcat gggcaaatca ggtatcagga aagcctatga aacaggtaaa ggatcgatta
    10561 ttgtccgtgg gcgtgtagaa gtcgaaatac tgaaaaacgg caaagaacgc atcgtcattt
    10621 ccgaattgcc ttatatggtc aacaaggcga aattggtcga gcggatcgct gaactggccc
    10681 gcgacaaacg gatcgaaggc ataaccgact tggcggatga gtccgaccgc gacggcatgc
    10741 gtgtcgtcat cgatgtccgc aaagatgtca gcgccagcgt cattctgaac aatctgtaca
    10801 aactgacgcc gctccaatcc tctttcggct tcaatatgtt ggcgattgta aaaggcatcc
    10861 cgaaagtgct gggactcaag cagattctgg tccactactt ggaacaccaa gaagaagtta
    10921 tccgcagaag agccgcattc gacaaacgaa aagcggaagc ccgtgcgcat atcttggccg
    10981 gtttgcggat cgctttggat catatcgatg agatcgtagc gattctgcgc ggttcaaaca
    11041 atggcgatgc agccaaacaa atattcatcg acaaatacgg tctttccgag aaacaagcgc
    11101 aagccatttt ggatatgcgt ctagtgcgtc tgaccggttt ggaaagagaa aaagttgaag
    11161 cggaatatga tgcgctgatg gctctgatcg ctgatttggc tgatatttta gcgaatgagc
    11221 agcgtgtttt cgacatcatc tatacagaat tacttgaaat ccaagaaaga ttcggcgaca
    11281 aacgccgcac ggagctgttg gtcggagaag tcttgtcgct tgaggatgaa gacttgatcg
    11341 aagaggaaga catcgtcttg actttgaccc acaacggcta catcaaacgt ttgccaaaca
    11401 gcgaattcag agcccaaaaa cgcggcggac gcggtgtcca aggtatgggt atccatgatg
    11461 atgatttcat cgaaacgctg atttcggcat cgacgcatga tgtgttgctg tacttctcga
    11521 accaagggaa agtatacaaa acaaaaggct atgaagtgcc tgagtacggc cgccaggcaa
    11581 aaggtttgcc gatcattaac ttgctgaatt tggatgccaa cgaaaaaatc caagccatca
    11641 tcaacgtcaa aggcggccca caagacggag attacttgtt cttcacgaca cggatgggta
    11701 ccgtcaaacg gacatccgct acggaattcc agaatatccg tcaaagcggt ctgcgcgcaa
    11761 tcaacctgaa ggatgaggat gagctgatca aagtctcctt gactgatggc aaccagaaca
    11821 tcatcatcgg aacgcacttc ggctactccg tgagttttga tgagaaggat gtccgcgata
    11881 tgggcagaac tgctaccggt gtccgtggcg tgaaattgag agaaaacgac tatgttgtcg
    11941 gcatggatgt gctgaaacac gagtcggaag ttctcatcat cacagaaaat ggctacggca
    12001 agcgtacggc agctgatgaa tatgctatca aaggccgcgg tggtaaaggg gtcaaaacag
    12061 ccaacatcac tgagaaaaat ggtaagttgg tgggcttgac gactgtctcc ggtgaagaag
    12121 acatcatgat cattacagat aaaggtgtca tgatccgttt ccacgccgat gccatttccc
    12181 aaacaggccg tgccactctg ggcgtgcgtc tgatcaagct tgatggcgaa gctatcgtat
    12241 cgacaatggc aaaagttgag cgcgaagagg atgaagaagt tgttgctgct cccctgaatg
    12301 aagaaataga ttcagagggc gaaaatgacg ccttagaagc atttgatgca actgaacagg
    12361 caaatacatt agatacaacg gaaacatccg aaatggccga taaattgagc gatttcgcag
    12421 atgaactgtt ggatgaagaa gattcggaag aataaagcgt caaaaacgaa actaattcag
    12481 aacggggccg tctcagtatg agatagcccc gttttttagt gtactttgga tttcggctag
    12541 ttgcctgctg ccacagagga gctgcaggat cggactgttg ctccggtgta gcggctgttc
    12601 atcggaggac agagaccgtt attggtcgaa cctccgataa agccgatcct accgaaggaa
    12661 agcaatataa tgtttgccta acttcggcga agctttcgtt cgccggagca ggagtaaaaa
    12721 ttcacagata tgattgcgga ctgcccagcg gaggctggat tcatttgtgt gagttacccc
    12781 cagttccgaa ttcatggcaa aaaaaagctt gccataaaaa atggaatatg atacaatctt
    12841 tagatgtgag taatcctgaa aaggtactct ccttgctctt acaaaagtaa gggccaaagt
    12901 ccacaaggag gtgacagtca aatgaacaat tacgaaatca actacattat ccgtcctaac
    12961 atcgaagaag aagctaaagc agaattgatc gcacgtttcg attctatttt aacagataat
    13021 ggcgctgaag ttattgaatc aaaagactgg gcgaaacgcc gtttagctta tgaaatcaaa
    13081 gattttcgtg aaggtatcta ccatatcgtg aaaatttctg cttcagacgc taaagcaatc
    13141 gacgaattcg accgtctagc aaagatcaat agcgatattc ttcgccacat tattgttaaa
    13201 gaagaagcta aataaagtgt tccacgtgaa acattttgaa aggagtaaga tgaatgatta
    13261 ataatgttgt cttagtcggc agacttacaa aagatgttga tttacgctat acatccagcg
    13321 gcacagcagt cggaaccttc gctcttgcag taaacagaca gtttacgaac caagcgggag
    13381 aaagagaagc tgacttcatc aattgtgtca tgtggagaaa atctgcagag aatttcgcaa
    13441 atttcactcg taaaggctct ttagtcggta tcactggccg aatccaaaca aggaattatg
    13501 ataatcagca aggacagcgc gtatacgtga ctgaagttgt tgcagataat ttcacattgt
    13561 tggaatcgaa aacaactacc gaacaaagac cgagagagac tggcgcaggt tcttcaaatc
    13621 aaggagctcc gagtgcaaac ttcggatcaa gcaaatcttt tgaatctaac caaaaccaag
    13681 gacagtataa taatcaatac tcttctccga aacaaaacgg tttttctgat tttaactcaa
    13741 ctgccgatcc gttttcatca aacggagaat caattgacat ttcagatgat gatttgccat
    13801 tctagtcttt agggcagaat ggttagttta ggaaaaggag gaattgacat ggcatttcaa
    13861 cgtaacagca gaggcggacg caaacgtaaa aaagtttgtt acttctgtgc taaccacatt
    13921 gatcatgtag attacaaaga gactgattta ttgaaacgtt atatatctga aaaaggtaaa
    13981 attttacctc gtcgtgtaac aggtacatgc gctaaacatc aaagaacttt gactgtatct
    14041 atcaaacgtt ctcgtatcat ggctcttatg ccatttacat tagcagaata agattaaggg
    14101 agtcgcttcc aggagcgctc ctttttttgt acccaaaaat agttgctgaa cacgaacatg
    14161 tcttgcagcg gatcggatat cacgagactc ccctttcctc ctctttcggc tacaactttt
    14221 ttggacacgc cctatccgtt tcttttgaac gatcagtatg ctcaggtgaa gcaaacagtt
    14281 tgcttcatct ttccgttccc agtgtctgct cccagctctc ctttggcatt gctcaaccgg
    14341 ctgaggatag actatttttt ttcaggttga gagtggatca ttcaaataca ttcctcgctc
    14401 aatagatgtg atactcaatt tacatgccct ctccttcatg ctagcagaaa gctttctcaa
    14461 ccttttatcc atacctggct gttgcaatga gtgattattc tccatcggag gcataggact
    14521 aagaactaaa atgatctgac cgggtcattt tacgcttgta agaacgaatt aacattatac
    14581 tagggtaaat cttcatagaa gaatacaggt gctgtcataa gcttaaatat agccgaattg
    14641 aataaagaaa aatcgccagc gctcaaatgt tccacgtgga acatttgagc gctgatttga
    14701 tttgaaaaca gttttcagat cgctacaatc tgaaagcaga aagaactgag ttatgggaaa
    14761 aacccgaggc gtgcaatcca gaaaaaataa aatcttccac tccatcatcc gtcctatggt
    14821 tatcccgatc agtaaatcga tacaatgata gaaggcctgc tgatgatgga tcgtaaaaat
    14881 caacaacaat gaatgagcca gaaaatagtg aatcggcttc aataaagaaa atgcataaac
    14941 ccgcttcaac aaatataaaa gtggaagcag ctgctcaaga caaggcaacg tagagctgaa
    15001 ggaacaacag tactccccta tcatcgccaa tgccatataa caaaccgacg ctatgggcca
    15061 agaaattgga ttccttctgg tataaattga aaagaatttg agggccagca tcaaacgcgt
    15121 tcaaacatca atggcaaaag ttggcgattt tgataccata cataggctta gaacgtatga
    15181 gtagatgatc aggtttgcca tgaaaaaaat tcacacgcaa atctgaactg aggcaagtat
    15241 catatctcaa gataaaaata gatcgagact attcaaaata tatcagatcg gtagtcgcat
    15301 atttccgtcc agacggttat tcctgagagg ccatcgcatt tcaagaatgg attaattaat
    15361 aattttcata aaatatgagt ccgagacttt attcatataa atcgaaacta ctcgagtcaa
    15421 gcgatgtttt ttctacgaat tgagaaattt gtatcgtatc atcccttcta aaagacgaaa
    15481 aagaggaagt ctattcagac aagatctcct ttatgatgat tgggcgattg cgggggaacc
    15541 aaaatttaaa tcacgtgctg taagagtaaa taactccctt ttacatacta tactaatgag
    15601 atggttttat agacggacgc atgtgataac ctgaaaaggc tccgtggatt gtacacaggc
    15661 tgattatatg tttattcttc aattatccac aaaaggatat gacgatgatt ttgagaccaa
    15721 tagtacattc aaagatagga tgtggaaaac tatttcctga actttatcca cagcggatgc
    15781 tggtatgcat ttgcaaacga gcgggaatga ctaaatgctg gagataagag tgaattcttc
    15841 aaaatccagc ttttttcata aaaatggtaa taaattcaca caaaataaag ggtttcattt
    15901 catcaaaaca cttatcaaac aaatgttcag aatgaaatga aggaacccgc acacttaact
    15961 cgacttatgc acaagatatc cacaggtatt cgagagtagt aaaggtgtca tgaatagtat
    16021 cagaaaaata agttatcaac aaagtcattt tatgacagaa atcctactgc agcaaggtta
    16081 tgatgtggta attattttag ttatgcacag ggcggcgtcg gaaaatgtgc ataactaaaa
    16141 aggttgcgga tagtttgagt tatccacaaa aacaggagct actacaatga aatgaagctg
    16201 gggagaagtg agaggatgaa aatccatttt caattacgga aatggatact gcagattata
    16261 tgaaaaaaaa ggagaaaatc gtatagctag attattcaaa tcattagtga aatagtaata
    16321 gaagtcatcg cgaaaatcgt tttttgtaaa aaaactaata gaataataaa agtgaaagag
    16381 agcaagtcta tgcgaaaaaa tagaaaaagt agagatcaaa actaaaaacg ttccacgtgg
    16441 aacattggtc aaaaaataaa cgtatacaga atttgaggat aaattgaaga gcgtaatcaa
    16501 tcaatgtttc acgtggaact attgaatagt agaaggagtc gcattcaact tataactata
    16561 ataataatta catttatatc gaaaatggta actattttga aagataaatt ttcacgacat
    16621 gcaaatccag taatagagaa ttacttatcc aaaatgcagg tatgctttga aaaaatatcc
    16681 aaaaaaatac agcattataa tccaatctag ccgagggtct aatgaacgct gaatgataac
    16741 agttgagaca tcgattattg agcacagata tcatcactgg gtaaatgaat acttttcaat
    16801 gcctgaaata tagcaacaat cgttctcgct cagaaaacct gctctccgca atacgtactt
    16861 tatacagagg aaatgtttca cgtgaaacat tcattttgac gctaaaatgg taactaatcc
    16921 ctctggaaag ggcattatag ctattattat ttgaatatgt taaaatgtac aagtgcaaat
    16981 tgtaggttca gtaaaggagc tatcatgaaa ataaaggaaa agttaaaaaa tcttcctcac
    17041 ttcataaagg ctgacaagat taaggttcct ttcctgatta tcgtattctt actgcttatg
    17101 attgttgttt taactttcat tgcagattgg aaaatcggaa ttggaattat gctcttatgc
    17161 gttggaattt ttgggttcat cagttattct tcggagtatt tgattgacga gactaatcaa
    17221 tacatatctg acttatctta tcgaataaaa aaaggtgaac aagaggcttt aattaaaatg
    17281 cctattggga tacttctata taataagcaa gaagaaatcc aatggatgaa cccatattta
    17341 ataaagtatt ttggcaagaa agacacattg ggacgtaagc tgaacgtggt cgatgaagaa
    17401 ttatatcagc tttttaaggg ttcgcaggat tatgtgatgc gccgtgtgaa atggggagat
    17461 cattattttg atatgattat tcaagaggat attggtgtca tttatttgat ggacataacg
    17521 gagtatgcca aaattgaaga aagatacaaa gatgaaaaat ttgtttttgg gaatatcatt
    17581 gtcgacaatt atgatgaggc tgtccaatcc atgaatgatc gcaagcgatc gaatgtaaac
    17641 aactatctga cgaaccaact cacaaactgg gctaatatga ataatgtttt tttgaaacga
    17701 gtggatgatg accgcttcat agcattgacc aatatgcgtg cattgaagaa aatcgaagaa
    17761 aacaattttg atatcataaa tcaaatcaga gaaagaacct acaagcaaaa ttttccgttg
    17821 acattgagta tgggcttctc atatgagaaa acttttgagc aaaccaattt taaagaagtc
    17881 gctaaaatgg cgcagtcgaa tattgattta gcattggcaa gaggtggcga tcaggttgtt
    17941 gtgagggctg aagatgaaga tgcccgtttc tatggtggta aaacgaatcc gatggaaaaa
    18001 cggactcgta tccgtgcaag aatgatcagc caggctttgg aagaactcat cgttcatgct
    18061 gataaagtga tcatcatggg gcatagttat cctgatatgg acgtcattgg ttcctgttta
    18121 gggatccgcc gaatctctca gatgaacaat cgggaggctt ggatagtatt ggatccgcag
    18181 caattttcca atgatgtgaa tcggattatg caggagatag acaaagatcc tttgattgcg
    18241 aagcgaatca tttcgcctga gcaggcaatg gcattggtga ctccgcaaac gcttatcgtg
    18301 atggttgacg tgcatcgtcc ttccatgaca ccggcccctg gacttttgaa tctgtccaaa
    18361 aaaatagtcg tcttggatca tcacagacgt ggacaagaat ttcccaaaga ctctgttttg
    18421 gtctatatgg aaccctatgc gtcatccact tcggaattga tcgctgaatt gtttgaatat
    18481 caatccaatg agatggatgc catcaataag atagaagcaa cggctatgct ggctgggatt
    18541 attgtcgata cacgcaattt cagcttgaga accggatcca ggacttttga tgctgccagt
    18601 tatctgcagt cgaatggagc ggataccgtt ctgatccaga aatcattgaa agaggacttg
    18661 gatacctatc tgttgcgtag tcatttgctg caaacgatgg aatttgtgaa cggtaacctc
    18721 gcaatcgtcc atggagaaga tgaaaaaatc tatgatacgg ttgtcgctgc tcaaacagcc
    18781 gacacgatgt tatcgatgga aggagtagac gcctcatttg tcgttacaaa acgtcaggac
    18841 ggcagagttg gaatcagtgc cagaagtctg gggcaatata atgtgcagac tatcatggaa
    18901 aaattaggcg gaggcggaca tttatccaat gcagccacgc agattgagaa tacgacggtc
    18961 catgaagtcg ttgagagatt gaaggcagtc atagatccaa aaaaaatcaa ggaaggggaa
    19021 taataaatga aagttgtatt tttagaggac gttaaaggta aagggaaaaa aggggaagtc
    19081 aaagatattg cggatggcta cgctaatttc ctgatcaaaa accgtaaagc ggaagaagcg
    19141 aatgctgcta cgatttctgc attgaaaggc caaaagaagg ctgaagagaa gaagcaggct
    19201 gaggagttag ccgctgctca agaattaaag gcacttctgg agaaggatga atcaatcatc
    19261 aaattgtccg caaaaattgg cgaagatggt cgtttgtttg gttcgataac aacgaaacag
    19321 gttgctgaag cattacagaa gcaacataat gttaaattgg ataagcggaa aattgaatta
    19381 gacagtccta tccgagcatt aggtttcacg aaaatgcccg taaaaattca tccggaagtg
    19441 acagcgatgt tgactgtcca agtgactgaa ggataataat tttccagata taaacgaaag
    19501 acagggataa ccatctctgt ctttttgctt gtgtagaggg ggataaatat tgattcgaaa
    19561 tagaacaagc gttccctttc gttggttgta aaagcctcca gtttacagta caataagaga
    19621 gcgaaagatg ggtaaaccag taaccggttc cttgtcgaaa tgaataaaac ctaaagggtg
    19681 aaaatataaa atggaagaaa cacttcaaga tcgcatacct cctcatagta tagaagcaga
    19741 gcaatccgtt ctgggctcca tttttttgga tcctgaaacg gttgtgaatg ttttggaata
    19801 ccttgaaaca tctgattttt accgaaaaaa tcatcagatt atttttgacg cgattttgca
    19861 attgaacaac cgaaacgaag cgatagacgt cgtcaccatc gcgaatgagt tggatacgaa
    19921 gaatcagctt gaaaatgctg gcggtatgga gtatttggcc gaattggcgg ttgcggtacc
    19981 gacttcagcc aacgtggaat attacgccaa gatagtagaa gagaaatcga ttttgcgcaa
    20041 cctgatccgc tcggctacag aaatcgtccg caaaggctat gaagagggtg atgagcttgc
    20101 cgtcatgttg gacagcgccg aacagaatat tcttcaggta tccgaacgga gaaatcgctc
    20161 cggtttcatc cgcatatcgg atgtcgtttc ggcgtcgctg cagaacatcg agtcgctggc
    20221 ccaacaaagc gatgacgtca caggggtgcc caccggatac atcgccttgg ataaaatgac
    20281 ggcgggtctt cagaaagaag aattgatcat cctggcagct cgtccggcgg tcggtaagac
    20341 cgcctttgct ctgaacatcg cacagaatgt cgcaaccaag gcggatcagg tagttgccat
    20401 tttcagtttg gaaatggggg cggagtcgtt ggtcaaccgg atgctgtgtg cggaagggaa
    20461 catcgatgcc ggtcacttga ggacagggca gctttctgaa gatgagtggt ccaacctgat
    20521 catggcgatg gggactttgg gacagtccaa gatttttata gatgatacac caggtatacg
    20581 gattgcggaa ataagggcaa aaagtcgtcg cttgctgcag gaacagggaa aattaggact
    20641 tattgtgatt gactacttgc agctgattga agggaacaac cgcgaaagcc gtcaacaaga
    20701 agtttcagac atttcgcgtc aattgaagaa gttggcgaag gaactgaaag taccggtcat
    20761 cgccctttcc caattgtccc gtggcgtgga gcaaaggcaa gacaaacgtc cggtattgag
    20821 cgatatccgt gaatcaggat cgatcgagca ggatgccgat atcgttgctt tcctgtaccg
    20881 cgacgactat tacgaacgtg aaggcggcga agaaccggaa cgggatgagc aagagaataa
    20941 tgtcgtagag gtcatcatcg aaaaaaaccg gagcggggcg cggggaaccg tcaagctcct
    21001 gttcaaaaag gaattcaata aattttcttc gatgagctat atgccggaaa ttagttgata
    21061 tttttggaca aattgttcag aaatgagcgc ttttatctga ttctttattg aaagacatag
    21121 gtaatattgg taaactagca tagtattatg aataaattag atgaggtgga gtaaataatg
    21181 acttcagtag tagttgttgg gactcaatgg ggagacgaag gtaaagggaa gattacagat
    21241 ttccttagtg agaatgcaga agtaatcgct agatatcaag gcggggacaa tgctggccac
    21301 acaatccagt ttgatggcgt tacatacaaa ttgcacttaa ttccatccgg cattttttat
    21361 ccagaaaaaa tcagcgtgat cggaaacggc gtagtagtga atccgaaatc attgatcaaa
    21421 gaattggcat atttgaaaga tcacggcatc aacacggata acctacgtat ttccgaccgt
    21481 gcccacgtca ttttgcctta tcatattcaa ttggatcagc ttcaagagga ttcaaaaggc
    21541 gaaaacaaaa tcggtacgac catcaaaggc atcgggccag cttacatgga taaagcagcg
    21601 cgtgtcggta tccgggtagc cgatttgttg gacaaagaaa tctttgaaga gcgtctgaaa
    21661 atcaacttgg aagaaaagaa ccgtcaattc acaaaaatgt tcgattctgt agaaatcaaa
    21721 ttcgaagata tttttgaaga atattatcag tacggacaag aaatcaaaaa atatgtgtgt
    21781 gatacatcgg ttatcttgaa cgatgtattg gatgcaggca aacgtgtcct atttgaaggc
    21841 gcacaagggg ttatgttgga tatcgaccaa ggtacttatc cattcgttac ttcttccaac
    21901 ccggttgctg gtggcgtaac catcggtagt ggtgtgggcc ctaccaagat cgataaagtt
    21961 gtcggtgtct gcaaggctta cacttcccgt gtcggcgatg gcccgttccc aactgaattg
    22021 ttcgacgaag taggaaacca aatccgcgaa gttggccgcg aatacggaac aacaactggc
    22081 agaccgcgcc gtatcggctg gtttgatgca gttgttatgc gtcattcaaa acgcgtttca
    22141 ggtatcacca acctatgctt gaactcaatt gatgttttga gcggcttgga aacagtgaaa
    22201 atctgtaccg cctacaaaaa tgctgaaggc gaattgattt accactatcc agcaagcttg
    22261 aaggaattgg cgcaatgcac gcctgtctac gaagaacttc caggctggag cgaagacatc
    22321 actggttgcc gtactttgga agagcttcct gaaaatgcgc gcaactatgt ccgtcgcgtt
    22381 caagaaaccg ttggcgtccg catctccact ttctcagtgg gacctgaccg caatcaaacg
    22441 aatgtattgg aaaacgtttg ggcaagtatc taatctagcg atgaacacaa tgaagagtcc
    22501 accccggaga acctatccgg atgtggactc tttttttcat aatgttattg cattttattg
    22561 aaaaggggag taaaataaca tttgttacgt gttcagtacg tgataggggt aattagctca
    22621 gttggcagag cagctgactc ttaatcagcg ggtcgggggt tcgagcccct cattacccat
    22681 aaatcttaac aaaaaatcct tggaacattg aagtgcttgt cttcaatagt ttccaaggat
    22741 tttttttcaa atataataca gaatgacgaa gatgggcatg gttacgatac atagcagcgt
    22801 ggtcatgatg ttgatcgcac ctgcgtatgc tgcattcttg tcgtgcacct gcgcgaattg
    22861 cgtcacagtg gaggctgcgg gggtcacggt ggccaggaag gtgatgagta atatttccgt
    22921 gccgttattc accaaactag cggcattgct gagtttcagc agcaatagga tgatggccgg
    22981 caagcggctt cccgacaaca tggacagcac gccaataaaa atggcg
//
LOCUS       DAOMUJ010000002        15849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_752_length_15849_cov_2.801064, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000002 DAOMUJ010000000
VERSION     DAOMUJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_15849_cov_2.801064"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..334
                     /locus_tag="PLT73_00105"
     CDS             17..334
                     /locus_tag="PLT73_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRM18813.1"
                     /translation="MKKLTKSATDRQVSGVLGGISEYFGIDSTIVRVIFLIAVFAGVG
                     SPVLLYILMAILMPEPGQSGSGQSFGGTYGSSSSRRSSDASRPVKEAKPVDKQEEDDW
                     SDF"
     assembly_gap    439..490
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            501..641
                     /locus_tag="PLT73_00110"
     CDS             501..641
                     /locus_tag="PLT73_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM18814.1"
                     /translation="MINGFMLYLTSWLVGPGFHFTSYGTAFLVALIMSIVHMFLSRDD
                     AH"
     gene            868..1806
                     /gene="hprK"
                     /locus_tag="PLT73_00115"
     CDS             868..1806
                     /gene="hprK"
                     /locus_tag="PLT73_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM18815.1"
                     /translation="MTNTVTVKELVDSSGYVVLSGEEFLGNKITTSEISRPGVELTGF
                     FNFYPSARIQLLGKTELTFIERMTAEERLIVMRRLCNKETPCFVIGRRLPAPSELTKA
                     AQEAGIPILSAQSRTTRVSSNITNFLEGKLAERVSMHGVFVDVFGMGVMITGDSGVGK
                     SETALELIQKGHRLVADDRVDLYQHDENTLIGEAPGILRHLIEIRGIGIIDVMTLFGA
                     GAVKQANEVNLIVNLELWSKDKKFERLGSTEEIVNILDVGIPKITIPVKTGRNLAIII
                     EVAAMNFRAKTMGYNAAETFERNLEALIKENTQKGE"
     gene            1827..2663
                     /gene="lgt"
                     /locus_tag="PLT73_00120"
     CDS             1827..2663
                     /gene="lgt"
                     /locus_tag="PLT73_00120"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRM18816.1"
                     /translation="MTNLIAALNPVALSFGGLEIRWYGVIIAAGILVAMALATKEAEK
                     KGLDPDFIVDMMFWTIPIGIIGARIYYVLFELDYYLQNPGEIIQVWNGGIAIYGALIA
                     GILTIYWYTKRKGISFALTMDILAPAVLIAQSIGRWGNFMNQEAHGEEVSRVFLENLF
                     LPEFIIEQMNINGIYYHPTFLYESLWSLAGFVLILFLRRKEQLLRVGEVMFTYVMWYS
                     FGRFFIEGMRTDSLYLFGPIRVSQALSILLFLGAMVYWAYRRRNNYPPDPYYTDISRK
                     RV"
     gene            2671..3321
                     /locus_tag="PLT73_00125"
     CDS             2671..3321
                     /locus_tag="PLT73_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM18817.1"
                     /translation="MEFKTILFDFDGTIADTNRLISESHFVVMEENFPGRFKMKEMAQ
                     FNGPSLEEIYGRLDQDRKDELVARYREVMLEKHDEMIGLFPGIKEMLGNLKQEGLRLG
                     VVSTKRTDVLKRGISILGITDYFDTIIGSGDFSQPKPDPESLFLAMERLEAKRETSAM
                     VGDNHHDIVAGNNAGITSIFVGWSEKTTAFIQPYQPTKIVKNPHELEEYILSGVRV"
     gene            3341..4387
                     /locus_tag="PLT73_00130"
     CDS             3341..4387
                     /locus_tag="PLT73_00130"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRM18818.1"
                     /translation="MRQKVAVLGAGSWGTALSMVLDENGHEVRLWGNDPQQIKEINEQ
                     HTNEHYLPGAKLSESIVAYTDLKAAIGDADALLFVLPTKAIRVVAKEVASLLDGQTRP
                     HLIHASKGLEQDTHKRISVIIQEEIPTEKYDSLVVLSGPSHAEEVAKKDITTITAASQ
                     DEEDAAYVQKLFMNPYFRVYRNTDVIGVELGAALKNIIAVGAGALHGLGYGDNAKAAL
                     MTRGLAEISRLGIAFGADPMTFFGLSGVGDLIVTGTSRHSRNWRAGDLIGKGQDLDEV
                     LNNMGMVVEGVATTKAAYELAQEKGIEMPITEAIYKVLYEKVDVKKAIEELMLREGKA
                     EQSSQNVEKGSQSI"
     gene            4384..5277
                     /gene="galU"
                     /locus_tag="PLT73_00135"
     CDS             4384..5277
                     /gene="galU"
                     /locus_tag="PLT73_00135"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRM18819.1"
                     /translation="MKKVRKAVIPAAGFGTRFLPATKAMAKEMLPIVDKPTIQFIVEE
                     AIASGIEDILIVTGKSKRPIEDHFDSNIELEANLREKGKNELLELVQETTGLRIHFVR
                     QSYPLGLGHAVLQAKAFVGDEPFVVMLGDDLMEDEVPLTKQLMDAYERTHASNIAVME
                     VPHKETSKYGIIDPDQELEPGLFNVRRFVEKPKPEDAPSNLAIIGRYLLTPEIFEILE
                     NQNPGAGGEIQLTDAIDTLNQTQRVFAKRFDGKRFDVGDKFGFLTTSISYGLTHPEIK
                     DKLKNYLVELGEKLTAEDEGK"
     gene            5495..6466
                     /gene="trxB"
                     /locus_tag="PLT73_00140"
     CDS             5495..6466
                     /gene="trxB"
                     /locus_tag="PLT73_00140"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM18820.1"
                     /translation="MEQTETIYDVIVIGAGPGGMTAALYASRSNLKTLLLERGIPGGE
                     MNNTAEVENYPGFNSILGPELAERMYESTMQFGAEHVYGDVKRIVVDGDYRIVEAGKK
                     TYKTRAVIIATGSYHRKLGVPGEAQYNGRGVSYCAVCDGAFFKGKKLAVVGGGDSAVE
                     EGTYLTQFADVNIVHRRDELRAQKVIQDRAFKNEKVSFTWNAIVEEIKGDGQKVTGVV
                     IRNKNTNEITEEAVDGVFIYVGILPQSDAFLDLGITDEEGWIITNEHMETAIPGIFAI
                     GDVRQKLLRQITTAVGDGGEAGNRAFAYIEDLHDRLAAAEADAETVD"
     gene            6635..8362
                     /locus_tag="PLT73_00145"
     CDS             6635..8362
                     /locus_tag="PLT73_00145"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM18821.1"
                     /translation="MSYQETIEQWNDFALLEPTLKEELKSLEGNEEALKDAFFAPLEF
                     GTAGMRGILGVGINRMNIYTVRQATEGLARLMEAKGEEAKKRGVAIAYDSRHQSPEFA
                     MEAAKTLGAHGIPAFVFESLRPTPELSFAVRHLNALTGIMITASHNPADYNGYKVYGD
                     DGGQMPPADADALTDYVRAIENPLTIQVGDEAELKAAGLIKMLGEEVDATYLDLVKTV
                     TVDPALVHEMSKEMKLVFTPLHGTGQMLGERALKNAGFEGIYLVPEQAVADPNFSTVK
                     SPNPEEAGAFEYAIKLGTELDADILVATDPDADRLGAAVRKVKGEYVVLTGNQIASLM
                     LDYLLRAKKAAGTLPANGTALKSIVSSELPTAIAKSYGADMVDVLTGFKFIAEKIKQY
                     EEDHSKEFLFGFEESYGYLAKPFVRDKDAIQALVLIAEVAAYYKKKGQTLYDGLVEIF
                     ETHGYYKEQTISVTMSGITGAEKIKALMAQFRTEAPADFAGIAVSITEDFGNSTKTFS
                     DGSTESIDMPSSNVLKYFLEDGSWIAIRPSGTEPKIKFYIGAKAESQAAVDEKVAAFE
                     TSIRTLTAE"
     gene            complement(8428..9048)
                     /locus_tag="PLT73_00150"
     CDS             complement(8428..9048)
                     /locus_tag="PLT73_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HRM18822.1"
                     /translation="MARVLVVRAHPFDSSVSRSMKVLDAFLEEYKALNPYDRIKDMNL
                     YEVAIPEIDRDLLTAWKELSKGVLFDQLTSRQQEKVTLFNHYTDDFVSMDKIIIANPL
                     WNMHVPSRLKTWIDTITVAGQTFRYTESGAVGMVPDKKLLHIQANGGIFDGSDPASQY
                     IKSIFHFLGVEEISQLFVEGMDTYPDRADEIVADAIEKAKQLAKTF"
     gene            complement(9438..11315)
                     /gene="asnB"
                     /locus_tag="PLT73_00155"
     CDS             complement(9438..11315)
                     /gene="asnB"
                     /locus_tag="PLT73_00155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRM18823.1"
                     /translation="MCGFVGYIHGSTAWNHQTVIEGMMNRIVHRGPDSGGMYSDDKVT
                     LGFRRLSIIDLSDNGKQPMYSADGNLVLVFNGEIFNFQELREDLKQKGHIFHSKTDSE
                     VLIYGYKEYGYDFVKQLRGMFAFAIWDKVNDTMFIARDGFGIKPLYYTTNTTDGSLLF
                     GSEIKSFLAHPAFIKEVNKDALRPYLTLQYSSMGETFFKGVHKLKPAHYMVLKGNDIH
                     TVEYWDKKFHAENGTLESYVEEVKATMADSVRAHKISDVKTGAFLSGGVDSSLVTALM
                     KPEKSFSVGFSEYEKMFNETNQAADLSEILGIENVRKYISPEEAFHALPTIQYHMDEP
                     QSNPSSVPLYFLAELAKKDVTVVLSGEGADEIFGGYSWYEPSKKMETYEKVPYSLRKG
                     IGKLAHKLPKNTLTTFLVKGGQKVEERFIGQAIVWDEEEAVDILKPAYKNGPDIRSIT
                     KPIYDEVKGQDDVTKMQYLDLNLWMPGDILLKADKMSMAHSLELRVPFLDKEVMELAK
                     RIPSDYRVNEIDTKYVLRRAAADVLPEEWAKRPKLGFPTPIRHWLREEEFYNEVRKAF
                     ASDYAAEFFDTDKLVQILDDNYTKKLDYGRQIWTAYIFLVWYKRFFIDETPLSSEAFI
                     A"
     gene            complement(11517..12194)
                     /locus_tag="PLT73_00160"
     CDS             complement(11517..12194)
                     /locus_tag="PLT73_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM18824.1"
                     /translation="MMNLSEMNTLQQLLYYYRENAGYVFEQFTRHFLISIYGVLFAAI
                     VAIPLGFWIARHKKLADWIIGAANVIQTIPSLALLSILMLGLGLGSDTVIATVFLYSL
                     LPIIKNTYTGVRNVDAALLDTGKGMGMTRMQLTYLVELPLSLSVIMAGLRNALVVAIG
                     ITAIGTFIGAGGLGDIISRGVNATNGTAIILAGAIPTALMAVLADWLLGLMEKRLDPS
                     SKVKRKR"
     gene            complement(12191..13141)
                     /locus_tag="PLT73_00165"
     CDS             complement(12191..13141)
                     /locus_tag="PLT73_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM18825.1"
                     /translation="MKKYMKKWLLFPLTALLLAACSLPGLGASVNNDGIVITGGTTTE
                     MQILSYMVRGMVEHYLPDASVDMVNNLGSSTLNHQAMIGGDANVSAARYTGTSLTGEL
                     AMDPITDPTLAFESVVKGFDDKFNQVWFPSYGFANTYAFLVTREFAEENNLKTVSDLA
                     ALAPNLRAGVDNSWMEREGDGYEAFKETYGFDFQRVYPMQVGLVYDALQADEMDIVLG
                     YSTDGRIASYDLVVLEDDLQLFPPYDASPVATKEILEAYPELEAILLKLEDTLTDELM
                     QAMNYEVDNDLVEAQTVAQTFLEENNYFEDKEVTPLKERK"
     gene            complement(13144..13782)
                     /locus_tag="PLT73_00170"
     CDS             complement(13144..13782)
                     /locus_tag="PLT73_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM18826.1"
                     /translation="MDFLKANGSELLFKTGEHFYISAMALVLGVLIAVPLGIALTRFK
                     RYSGFIISFVSILQTVPSLALLALMIPLFGIGKLPAIVALFIYSLLPILRNTFIGISN
                     VNSTIVDSAKGMGMTDNQIILQVKLPLAAPVIMSGIRLAGVYVIAWATLASYIGAGGL
                     GDYIFNGLNVYDQEMIIWGTLPVTLLALLVDFLLGKLERFVSPQTTSAKGGN"
     gene            complement(13787..14968)
                     /locus_tag="PLT73_00175"
     CDS             complement(13787..14968)
                     /locus_tag="PLT73_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710589.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM18827.1"
                     /translation="MIEFKNLTKRFPGGKIAVDSLNLTFNDGEFIVFIGTSGSGKTTS
                     MRMINRMIEPTSGEILINGKNSKDMNAVELRRQIGYVIQQIGLMPHMTIFDNIVMVPK
                     LLKWPVEKQRKIAEELIQKVDLPLDFLERYPSELSGGQQQRIGVIRALAADQDIILMD
                     EPFGALDPITRDSLQETLKELQRELGKTVIFVTHDMDEALKLADRIVIMQDGKVVQFD
                     TPDNILMDPANAFVENFIGEERLSQARTNFRTVEQIMIRGPVSVSSDQNLSEAIKLMR
                     TRRVDSLFVTDADDILLGMLSVEAIDTNRRRPVTVGEVMSEVSFVREGTLVRDALQRI
                     LKLGYKNIPVVNENNQLIGLITRTSIVDMVYDTIWGDIEPEVPAEEVIETTADIETVE
                     G"
     gene            15377..>15849
                     /locus_tag="PLT73_00180"
     CDS             15377..>15849
                     /locus_tag="PLT73_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010688305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRM18828.1"
                     /translation="MSIDQGTTSTRAIIWDKKGTIISSSQRELMQYYPEPGWVEHDAN
                     EIWISVQSVIADALIRGAIRPEEIAAIGVTNQRETTVVWDRVTGMPIHHAIVWQSRQT
                     SEIADKLKQEGHSDFIHDKTGLIIDSYFSATKIKWLLDHIPNARERAEKGELLFGT"
BASE COUNT         4362 a         3545 c         3943 g         3947 t
ORIGIN      
        1 taaggagaga acgaacatga aaaaattaac caaatctgct acagacagac aagtcagcgg
       61 tgttctggga ggtatttccg aatacttcgg catcgattcg acgattgtgc gtgtgatctt
      121 tctgattgcc gtctttgcgg gagtcggatc gcccgtattg ctgtatatcc tgatggcgat
      181 cctgatgcct gaaccgggcc aaagcggcag cggacaatca ttcggaggaa cgtatggatc
      241 atcatcttcc cgtcgcagtt ccgacgcttc aagaccagta aaggaagcaa aaccagtgga
      301 taaacaagaa gaggacgact ggagtgattt ctgatgggat tctggaaaaa agcagcggtc
      361 aactcgctag tgttcatcgc cttagcttat ttcatggcac cttccttcta tgtgcatagc
      421 ctttggacag cgttctggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn gttcagcttc gtgatcaatg gattcatgct gtatctgacg tcttggttgg
      541 tgggaccggg attccacttc acaagctacg gtacagcgtt tctggtggct ttgatcatgt
      601 cgatcgtgca tatgttcctg agcagggacg atgcgcatta gttaagtggg ttcaacaata
      661 gcagtcagta tcaaataacc taaatgaagg cagctgaaac gattgtttca gctgcctttt
      721 gttctttcgt atgcatggat aaaatgttat gatggatagg aatacaataa aacacaagcg
      781 attcaattat tgtttgagga gagatgaaaa agagaaaatc ctgcctgatc gaggtcagga
      841 aattaaatga tagagtgagg gataagaatg acgaatacag tcactgtaaa ggaattagtg
      901 gattcttcag gttatgttgt gctgagcgga gaagagttct tgggaaataa aatcacaacc
      961 agcgaaatct ccagacccgg cgtcgagttg accggtttct ttaattttta cccgtcggcc
     1021 cgtatccaat tgttggggaa aacggaactg acatttatcg agcggatgac tgcagaagag
     1081 cgcctgatcg ttatgagacg cctctgcaac aaagaaacac cttgcttcgt tatcggacgc
     1141 agattgcctg cgccctcaga attgacgaaa gccgctcaag aagctggtat accgattctt
     1201 tcggcacaat ccagaacgac aagagtatcc agtaacatca ccaattttct tgagggcaaa
     1261 ttggctgaac gcgtttcgat gcacggggtt ttcgtggatg tatttggaat gggggtcatg
     1321 attacagggg acagcggcgt cgggaagtcg gagacggctt tggaattgat ccaaaaagga
     1381 caccggttgg tcgctgatga tcgtgttgat ctgtatcagc atgacgaaaa tacgctgata
     1441 ggtgaggcac caggcattct tcgccatctg atcgaaatcc gcggaatcgg aattattgat
     1501 gtgatgacgc tgttcggggc cggcgcggtc aaacaggcta atgaagtcaa tctgattgtt
     1561 aacctggaac tttggagcaa agacaagaaa tttgaacgtc tgggcagtac cgaggaaatc
     1621 gtgaacattt tggatgtcgg catccctaaa atcacaattc cggttaagac agggcgaaat
     1681 cttgctatca tcatcgaagt ggctgccatg aatttccgtg cgaaaacgat gggctataat
     1741 gccgctgaga ctttcgaaag aaatctggaa gctttgatta aagagaatac ccaaaaaggc
     1801 gaatagcttg aacaaaggag attaacatga ctaatttgat agccgcacta aaccccgtcg
     1861 ccttgtcgtt cggcggtttg gagatccgtt ggtatggtgt catcatagcg gcagggatac
     1921 ttgttgccat ggcattggct acgaaagagg ccgagaaaaa aggactggat ccggacttta
     1981 tcgtcgacat gatgttctgg acgataccga taggaatcat cggcgcccgc atttactatg
     2041 tattgtttga attggattat tatttgcaga acccaggcga aatcattcaa gtctggaatg
     2101 gtggcatcgc catttacggt gcgttgatcg ccggtatttt gacgatctat tggtacacaa
     2161 aacgaaaagg gatttccttt gcattgacga tggatatttt ggcacccgca gttctgattg
     2221 cacaatccat cggcagatgg gggaatttca tgaaccagga agcgcatggt gaggaagtca
     2281 gccgcgtctt tttggagaat ttgttcctgc ctgaattcat tattgaacaa atgaacatca
     2341 atgggatcta ttaccacccg accttcttgt acgaatcgct ctggtcgttg gccggttttg
     2401 tgctgattct gttcctgcgc agaaaagaac aactgttgcg tgtcggtgaa gtaatgttca
     2461 cttatgtcat gtggtactcg ttcggacgct tcttcatcga aggtatgcgt acagacagcc
     2521 tgtacctttt cgggcctatc cgcgtttcgc aggcgctgtc catccttctg ttcctcggag
     2581 caatggtgta ctgggcttac agacgccgaa ataattatcc gcctgatccc tactacacgg
     2641 atatcagtag aaagagagta taatagacga atggaattta aaacaatatt atttgatttt
     2701 gatggcacta tcgccgatac gaaccgactt atcagtgaaa gccactttgt tgtcatggag
     2761 gaaaatttcc cgggccggtt caaaatgaag gaaatggctc agttcaacgg tcctagttta
     2821 gaggaaatat atgggcgtct tgatcaggac agaaaagatg agttggtcgc acgttaccgg
     2881 gaagtgatgc tggagaagca tgatgaaatg atcggacttt ttcccggcat caaagaaatg
     2941 cttggaaatc tcaaacaaga aggcttgcgc ttaggcgtcg tttcgacaaa acgtaccgat
     3001 gtgctgaaaa gaggcatatc cattttaggc atcacggatt attttgatac gatcattggg
     3061 tcaggcgatt tttcacaacc caagcctgat ccggagtcgc tctttctggc tatggaacgt
     3121 ttggaagcaa aaagagagac cagcgcgatg gtcggggaca atcaccacga tatagtcgca
     3181 ggcaataatg cgggaatcac aagtattttt gtgggctggt ccgaaaaaac gactgccttt
     3241 atccaaccct atcaaccaac aaaaatagtc aaaaacccac atgagttgga agagtacatt
     3301 ctttccggcg tgagagttta atggagggga agagatcaaa atgagacaaa aagttgctgt
     3361 actaggggct ggttcttggg gaacagcctt atcgatggta ttggatgaaa atggacatga
     3421 agtccgtctt tgggggaatg acccacaaca aatcaaagaa atcaatgagc agcatacgaa
     3481 tgagcattac ttacccggtg caaagctgag tgaatcgatt gtagcgtaca ccgatctgaa
     3541 agctgctata ggtgacgcgg atgcgctttt gttcgttctg ccgacaaaag caatccgtgt
     3601 agttgcgaaa gaagtcgcat cattattgga cggccaaaca aggccgcacc tgatacatgc
     3661 cagcaaagga ttggaacagg atacccataa acggatttcc gtcatcatcc aagaagagat
     3721 tccgacagag aaatacgata gcttggttgt cctttccgga ccgagccatg cggaagaagt
     3781 cgcaaaaaaa gacatcacaa cgatcactgc cgcatcccag gacgaagagg atgcagctta
     3841 tgtgcaaaaa ctgttcatga atccttattt cagggtctac cggaacacgg atgtcatcgg
     3901 agttgaattg ggtgctgctt tgaagaacat cattgccgta ggtgctgggg cgcttcatgg
     3961 attgggttac ggcgataatg cgaaggcggc tttgatgaca cgcggattag cggaaatcag
     4021 tcgtctgggg atcgctttcg gtgcagatcc tatgacattc ttcggtttga gtggcgtggg
     4081 cgacctgatc gtgaccggca ccagccgcca ttcgcggaac tggcgagcag gggatctgat
     4141 cggaaaaggc caggatctgg atgaggtgct gaacaatatg gggatggtcg ttgaaggtgt
     4201 agcaacaaca aaggccgctt atgagttggc gcaggaaaaa ggcatcgaaa tgccgatcac
     4261 agaagccatc tataaagtgt tgtacgagaa agtcgatgtc aaaaaagcaa tcgaggaatt
     4321 gatgctgcgc gaaggcaaag cggagcaatc ttctcaaaat gtggaaaagg ggagccaatc
     4381 aatatgaaga aagtcagaaa agcagtgatt cctgctgcgg gattcggaac gcgtttttta
     4441 ccggcaacga aagcgatggc aaaagaaatg ttgccgatcg tcgataagcc gaccattcaa
     4501 ttcatcgtcg aagaagcaat cgcatcaggg atcgaagata tcctgatcgt aaccggaaaa
     4561 agcaaacgtc cgattgaaga ccattttgat tcaaatattg aattggaagc gaatctgcgt
     4621 gaaaaaggca aaaatgaatt gttggaattg gttcaggaaa cgaccgggtt gcgtatccat
     4681 ttcgtccgcc agtcttatcc attggggctg ggtcatgcgg ttctgcaggc gaaagctttt
     4741 gtcggagatg aaccatttgt cgtcatgctg ggtgatgatc tcatggagga tgaagtgccg
     4801 ttgaccaagc agttgatgga tgcttacgag cgcacgcatg cttccaatat cgccgtgatg
     4861 gaagtgccgc ataaagaaac atccaaatac ggcatcatcg atccggatca ggaactggaa
     4921 ccaggcctgt tcaacgtccg cagattcgtc gaaaaaccga agccggaaga cgcaccgagc
     4981 aatttggcta tcatcggccg gtacttattg actccggaaa ttttcgaaat tctggaaaat
     5041 caaaatccag gtgcaggcgg agaaatccaa ttgaccgacg caatcgatac tttgaaccaa
     5101 acgcagcgcg tcttcgcaaa acgcttcgac ggcaagcgtt ttgacgttgg agacaaattc
     5161 ggtttcctga cgacaagcat ttcctatggc ctcacccatc cggaaataaa agataaattg
     5221 aaaaattatc tggtggaact tggtgaaaaa ctgactgccg aggatgaagg caaatagccg
     5281 ttttggtaat ccgaatccag ccgaatattg agtgataaac aatagaagcc gagccatcaa
     5341 tcgttctgcg atcaatggct cggcttcttc tcgctgactt tggagccatg agcagaattg
     5401 tggcacaaac aatcaagtca gagtaaacta tattagaacc agattccggc acctgaccgg
     5461 aacagacttg acacaagaag gagatacaaa aaatatggaa cagactgaaa caatttatga
     5521 cgtcatcgtc atcggagccg gaccaggcgg aatgaccgca gcactgtacg catcccgatc
     5581 aaatttgaag acgttattgc tggagcgtgg tattcccggt ggggagatga acaatacagc
     5641 tgaagttgag aattaccctg gtttcaattc gattttaggc cctgagttgg ctgaacggat
     5701 gtacgaaagc acaatgcaat tcggggcgga gcatgtttat ggcgatgtaa aacgaattgt
     5761 tgtcgacggc gattaccgta tcgttgaagc aggcaaaaaa acctataaga cacgtgccgt
     5821 catcattgcg accgggtcct accaccgaaa attgggtgtt cccggtgagg cgcagtacaa
     5881 cggccgcggc gtttcctatt gtgccgtctg tgatggagct ttctttaaag gcaaaaaact
     5941 agccgtggtc ggcggcggag attctgccgt ggaggaaggg acttatttga cgcaattcgc
     6001 ggatgtcaac atcgtgcaca gacgggatga gttgcgggct caaaaagtca tccaggatcg
     6061 tgccttcaaa aatgagaaag tttccttcac ttggaatgcc attgtggaag aaatcaaggg
     6121 cgacggacaa aaggtcactg gcgtggtgat ccgcaacaaa aataccaatg aaatcacgga
     6181 agaagctgtt gacggtgtct tcatctatgt tggtatcctg ccgcaatcgg atgctttcct
     6241 tgatttaggg atcacggatg aagagggctg gatcatcacg aatgagcaca tggaaacggc
     6301 gataccgggc atctttgcca tcggggatgt gcgtcaaaaa cttctgcgtc aaattacgac
     6361 tgctgtcggt gatggcggag aagccggaaa ccgcgcattt gcttacatag aagacttaca
     6421 tgaccgcttg gcagccgccg aagcggatgc tgagacggtg gactgaaaat attgtccaca
     6481 caacaacaat gttattgtcc agttcattag taccccgaaa gttcacgttc tgaaatcaga
     6541 gcgtgaactt tttttgtaga ttcaatcttt tcgctcaatt ggcatgcaaa tgatgttata
     6601 atatgattaa actaatatgg gaaggatgat gaaaatgtct tatcaagaaa ccatcgagca
     6661 atggaatgat tttgccttat tagaacctac attaaaagaa gaattaaaat ctttggaggg
     6721 aaatgaagaa gcactgaaag atgctttttt tgctccgctg gaattcggaa ctgccggaat
     6781 gcgcggcatc ctgggagtcg ggatcaatcg gatgaacatc tacacagtcc gccaagcgac
     6841 cgaaggcttg gctcgtttga tggaggcaaa aggtgaagaa gcgaaaaaac gcggcgtcgc
     6901 gatcgcttac gattccagac accaatctcc ggagtttgcg atggaggcag cgaaaacatt
     6961 gggtgcacac ggcattccgg ctttcgtttt tgaaagctta cgccccacac ctgaattatc
     7021 ctttgcggta agacatttga atgcgctaac ggggatcatg atcacagcga gccacaaccc
     7081 ggctgattac aacggttata aagtctatgg agacgacggc ggccagatgc cacctgccga
     7141 tgcggacgct ttgactgatt atgtgagagc aattgaaaat ccactgacca tccaagtggg
     7201 cgatgaagca gagctgaaag cagcgggctt gatcaaaatg cttggggaag aggttgatgc
     7261 aacttacctt gaccttgtca aaacagtgac cgtcgatcct gcccttgtcc atgaaatgag
     7321 caaagaaatg aaattggtct tcacgccgct gcatggaacc gggcagatgc tgggtgaacg
     7381 tgctttgaag aatgccgggt ttgaaggcat ctatctcgtg ccggaacagg cagtggccga
     7441 tccaaatttc tcaaccgtga aatcaccgaa tccggaagaa gctggcgcat ttgagtatgc
     7501 catcaaactt ggaacggaat tggatgcgga tattttggtt gcgaccgatc cggatgctga
     7561 ccgcttgggc gcggccgtcc gcaaagtaaa aggcgagtac gttgttctga cgggtaacca
     7621 gattgcttca ttgatgctgg attatttgtt gcgcgcgaaa aaagcggctg gtacattgcc
     7681 agccaacggc acagccctga aatcgatcgt atcgagcgag ttgccgacag caatcgccaa
     7741 atcttatgga gcggacatgg tggatgtact gaccggtttc aaattcatcg cagaaaaaat
     7801 caagcaatac gaagaagatc acagtaaaga attcttgttc ggcttcgagg aaagctacgg
     7861 ttatcttgca aaacctttcg ttcgcgataa agacgccatc caggccctcg tgttgattgc
     7921 cgaagtagct gcgtactaca agaaaaaagg tcaaacgctg tacgatggct tggtcgaaat
     7981 tttcgaaaca catggctatt ataaagaaca gacgatttcc gttacgatgt ccggcatcac
     8041 cggagcagaa aaaatcaaag cgctgatggc ccaattccgt acggaagcac cagcggattt
     8101 cgcaggcatc gccgtatcga tcacggaaga cttcggcaac tctacaaaga cattctctga
     8161 cggctccact gaaagcatcg atatgccgag ctcgaatgtg ctgaaatatt tcctggagga
     8221 cggcagctgg atcgccatcc gcccaagcgg aacagaacca aaaatcaaat tctatatcgg
     8281 tgccaaagcg gaaagccaag ccgcagtcga tgaaaaagtc gctgcttttg aaacaagcat
     8341 ccgtactttg accgctgaat aatccgaaat aaactaaaaa aaggaagtcc gaagattacg
     8401 gacttccttt ttgctgtgga ttgttcatca gaatgttttg gccagttgtt tggctttttc
     8461 gatcgcatcc gcaacgattt catcggcacg atccggatat gtgtccatac cttcaacgaa
     8521 tagttgtgaa atctcctcga cccccagaaa atggaaaatg ctcttgatgt actggctggc
     8581 aggatcgctt ccgtcgaaaa tgccgccatt cgcttggata tgcaacaatt tcttgtccgg
     8641 gaccattccg actgctccgg attccgtgta acggaatgtc tgaccagcaa ctgtgatagt
     8701 gtcgatccat gtcttcaggc gactgggaac atgcatgttc cataatggat tggcgatgat
     8761 gatcttatcc atggaaacaa aatcatcggt atagtgattg aacagcgtca ctttttcttg
     8821 ctgacgggaa gtcaactgat caaaaagcac gcctttcgat aattccttcc aggctgtcaa
     8881 aagatcccga tcgatctcag ggatggcgac ttcataaaga ttcatgtctt tgatgcgatc
     8941 ataaggattc aaagctttgt attcctccag aaaagcatcc aaaactttca tggagcggga
     9001 tacactgctg tcgaacggat gcgcgcgcac aactaaaact ctggccatag aaaaaccttc
     9061 tttccaattt ttatacctta atgataccat ttgcacgcaa agataagaaa gcacaaccca
     9121 tcaccaacag gatgattcgc tgaccaaaaa cggaagaggg gttcccggat gaattgaagc
     9181 agcaatcagc ggtctgttct cctctggcga acggaggctt cgccggagtt cggcccacag
     9241 gcagtatgcg tactccggtt aggggtggtt cgcgggagtt caggctgatt gtcggtggcg
     9301 ttctccggct aaagaccgct caccggagct aggcagcatt tcggacgcgc tcctccggtc
     9361 agctggccgc gcagaaacag gcgcacaaaa acaggagcct ccgcgctagg aggctcctgt
     9421 ttctgattta tgtaatctta agctatgaaa gcttccgaag acagaggtgt ttcatcaatg
     9481 aagaaacgtt tgtaccagac gaggaagata taggctgtcc agatttggcg gccataatcc
     9541 aattttttcg tatagttgtc gtcgagaatc tgtacaagct tatcggtgtc gaaaaattct
     9601 gctgcatagt cggaagcgaa agcttttctg acttcgttgt agaattcttc ttcgcgcagc
     9661 cagtgacgga tcggagtcgg gaagcccaac ttcggacgtt tcgcccattc ttcaggcagg
     9721 acatcggcag cggctctacg gagcacgtat tttgtatcga tttcgttgac acgataatcg
     9781 gatgggatgc gtttcgccaa ttccatgact tctttatcca ggaaaggtac gcgcagctcc
     9841 aacgagtggg ccatgctcat cttgtcggct ttcaacagga tatccccagg catccaaagg
     9901 ttcagatcga gatactgcat cttcgtcaca tcatcctgac ctttgacttc gtcgtagatc
     9961 ggtttggtga tcgaacggat atccggaccg tttttgtagg ccggcttcag gatatccaca
    10021 gcttcttctt cgtcccagac gatggcctgg ccgatgaagc gttcttccac tttttgaccg
    10081 cctttgacca ggaaggtcgt cagcgtattt ttcggcaatt tatgtgccaa tttgccgatg
    10141 ccttttctta ggctgtaagg gactttttca taggtctcca ttttcttgga tggctcatac
    10201 caagagtagc cgccgaaaat ttcatcagcg ccttcacccg aaagtacgac cgtcacatct
    10261 ttcttcgcca attcagccaa gaaatacaac ggaacggaag acggatttga ttgcggttca
    10321 tccatatggt attggatggt aggcaaggca tggaaagctt cttcaggaga aatgtacttg
    10381 cggacattct cgatgcccaa gatttcagaa agatcagcag cttggttggt ttcgttgaac
    10441 attttttcat attccgagaa gccgaccgaa aaagattttt ccggtttcat caaggctgtg
    10501 accaagctgg aatcgacacc gccggaaagg aaggcgcctg tcttcacatc actgattttg
    10561 tgggcacgga cggaatctgc cattgtcgct ttgacttctt ccacatagct ctccaaagtg
    10621 ccattttcgg catggaattt tttgtcccaa tattcgaccg tatggatatc gttgcctttc
    10681 agcaccatat aatgcgccgg tttcaatttg tggacgcctt tgaagaaggt ttcgcccatc
    10741 gaagagtatt gcagcgtcaa ataagggcgc aaagcatctt tgttcacttc tttgatgaaa
    10801 gcagggtgag ccaaaaatga cttgatttca gaaccgaaaa gcaagctgcc gtcggttgtg
    10861 ttcgtcgtgt agtacaaggg tttgatgccg aagccgtcac gggcgatgaa catcgtgtcg
    10921 ttcactttgt cccaaatcgc gaacgcgaac atgccgcgca gttgtttcac gaagtcataa
    10981 ccgtattctt tatagccgta aatcaaaact tcactgtccg tcttggaatg gaagatgtgt
    11041 cctttttgct tcagatcttc gcgcaactcc tggaagttga agatttcacc gttgaacacc
    11101 aataccaaat ttccgtcggc actgtacatc ggttgttttc cgttatcgga aagatcgatg
    11161 atgctcaagc ggcggaagcc taacgttact ttgtcatcag aatacatgcc gccactgtct
    11221 ggtccgcggt ggacgattct gttcatcata ccttcaatca cagtctgatg gttccatgca
    11281 gtcgatccat ggatatatcc aacaaaacca cacatttata ccactcctta agtatctctt
    11341 gctagatcag aatttctgtc cgtctagagt ccaatctatt ctatcacaaa tggaaaatca
    11401 ttaaaagaaa ttatccaatc gggaatctat acggaatatc caaaaatcaa aggacaagct
    11461 gttccacttt tcatggaaca gtcttgtcct tcgaattcag tgcttatatt ttgcgttcag
    11521 cgcttacgtt ttactttgct ggagggatcc agacgttttt ccatcagacc caacaaccag
    11581 tcggccagaa cggccatcaa agcggtcgga atagcaccgg cgaggatgat ggctgtcccg
    11641 ttcgtcgcat tgacgccgcg ggaaatgata tcccctaatc cgccggcacc gatgaaggta
    11701 ccgatggcag tgataccgat cgcaacgacc aaagcattac gcaatccggc catgatgacg
    11761 gaaagggata acggtaattc gaccagatag gtcaattgca tccgtgtcat gcccatgcct
    11821 ttgcctgtat ccaacaaggc ggcatcgaca ttccggacac ccgtgtaggt gtttttgatg
    11881 atcggcagca acgaatacag gaagacggtc gcaatgacgg tgtccgatcc gagtcctagt
    11941 ccgagcatca aaatggacaa cagcgccaac gaagggattg tctggatgac attggcggcg
    12001 ccgatgatcc aatccgccaa cttcttgtgg cgcgcaatcc aaaaaccgag ggggatcgcc
    12061 acaatcgccg cgaacagcac accgtaaatg gaaatcagaa aatgacgggt gaattgttcg
    12121 aatacatagc cggcgttctc tctataatag tagagcaatt gctgaagtgt gttcatttca
    12181 cttaagttca tcattttcgc tcctttaggg gtgtgacttc tttatcttcg aaatagttgt
    12241 tctcttccaa gaaagtttgc gcgacggttt gggcttccac caaatcgttg tcgacttcat
    12301 agttcattgc ctgcatcagc tcatccgtca gcgtatcctc aagtttcaga aggatggcct
    12361 ccagttcggg gtaagcctca agaatctctt tagtggcaac cggactggcg tcgtatggcg
    12421 ggaacagttg cagatcgtct tcgagaacga ccaggtcata gcttgcgatc cggccgtcag
    12481 tggagtagcc taaaacgata tccatttcgt ccgcctgcag ggcatcatag acaagaccga
    12541 cttgcatggg atagacacgc tggaagtcaa aaccatatgt ttctttgaag gcttcatatc
    12601 cgtcgccttc ccgctccatc caggagttgt ccactccagc ccgcagattg ggtgccaatg
    12661 ccgcgaggtc gctgacagtc ttcagattgt tttcttcggc aaattcacgt gttaccaaga
    12721 aggcatatgt gttggcaaac ccgtaggaag ggaaccaaac ctgattgaat ttatcatcga
    12781 agcccttcac aacagattcg aaagccaatg tgggatcggt gataggatcc atggccaact
    12841 caccggtcag agaagtccct gtatagcgag cggccgaaac atttgcatcg ccgccgatca
    12901 tagcttggtg attcaaagtc gatgaaccga ggttgttgac catatccacg gaagcatccg
    12961 gcagatagtg ttcgaccatc cctctgacca tataactcaa tatttgcatt tctgtcgttg
    13021 tcccaccggt gatgacgatg ccgtcattgt ttacggaggc acccagacca ggcagagagc
    13081 aagccgccaa taacagtgct gtcagcggga aaagcaacca ttttttcata tatttcttca
    13141 tcatcagttt cctccttttg cagatgttgt ctgtggagaa acgaatcgtt caagcttacc
    13201 gaggaggaag tcaaccaata aagccaacaa ggttaccggc agggttcccc aaatgatcat
    13261 ttcttgatcg tatacattca atccgttgaa aatgtaatcc cctaaaccac cggctccgat
    13321 atacgaagca agagtcgccc aagcgatgac gtacactccc gccaaccgga tcccggacat
    13381 gatgaccggg gcagcaagcg gcaatttcac ctgcagaata atctgattat cggtcatacc
    13441 catccctttt gcggagtcaa cgattgtgct gttcacattt gagatgccga tgaacgtatt
    13501 tctcagaatc ggcaagagtg aatagatgaa caaggctaca atcgcgggaa gctttccgat
    13561 tccgaacaag ggaatcatca aggcaagcaa agccagagaa gggacagttt gtagaatcga
    13621 aacaaaactg atgataaaac cggaatagcg tttgaaacgt gtcaaggcga tgcctaaagg
    13681 aacagcaatc agaacgccaa gcaccagcgc catcgctgag atgtagaaat gttctcctgt
    13741 tttaaagagc aactcggaac cattcgcttt caaaaagtcc atatcatcac ccctcaaccg
    13801 tttcgatgtc cgcggttgtc tcgattactt cttccgccgg cacttcgggc tctatgtctc
    13861 cccaaattgt gtcatagacc atatccacaa tggatgtgcg ggtaatcaag ccgataagct
    13921 gattattttc attgaccaca ggaatatttt tgtaacccaa tttcaagatc cgttgtaaag
    13981 catcccggac gagtgtgcct tcacgaacaa aggaaacctc actcatgacc tcgccgacag
    14041 tgactggacg gcgtcggttg gtgtcgatcg cttcgacact caacatgccc aacaagatat
    14101 catccgcatc ggtcacgaac aaactatcga cacgacgggt gcgcatcaat ttgatggctt
    14161 cagaaagatt ttggtctgac gaaaccgaaa ctgggccgcg gatcatgatc tgctcaacag
    14221 tgcggaagtt cgtacgtgct tggctcagac gttcttcgcc tataaagttt tcgacgaatg
    14281 cgttcgcagg atccatcagg atattgtcgg gtgtgtcgaa ttgaacgact tttccgtctt
    14341 gcatgatgac gatccggtct gccagtttca aagcttcatc catatcatgt gtgacgaaga
    14401 taaccgtttt tcccaattcg cgttgcagtt ctttgagggt ttcctgcagg gaatcgcgag
    14461 tgatcggatc caaagctcca aaaggttcat ccatcaaaat gatgtcctgg tcagctgcca
    14521 aagcccggat aacaccgata cgttgttgtt gcccgccaga cagttcggat ggatagcgtt
    14581 cgagaaaatc caaaggtagg tcgacttttt gaatcagctc ttctgcaatc tttctttgtt
    14641 tttcgacagg ccatttcaat agttttggaa ccataacgat gttatcgaag atagtcatgt
    14701 ggggcatcag gccgatctgc tggatgacgt agccgatttg gcgtctcagt tccaccgcgt
    14761 tcatgtcctt gctgtttttt ccgttgatca aaatttcacc ggaggttggt tcgatcatgc
    14821 ggttgatcat ccgcatagag gttgttttgc cgctgccgct cgtcccgatg aatacaatga
    14881 actctccgtc attgaatgtc aggttcagag aatccacagc aattttcccg cctgggaatc
    14941 gtttagtaag atttttaaat tctatcatgg tttcacctct tggtattttt aggttcacaa
    15001 atgtcttact ctaccttaac gcgcaacaaa gcctttttgc aaatgataag gttgaaatct
    15061 tgtgaaaaaa acgcacttcc ggaagcgtta ccattgttgc gatgacggta tgtttgcgac
    15121 aaatttttat atttttaaat ttacgcattc tgaatgtctc caaattgaca aacgtgacag
    15181 taaatatagg gttagagata acccggaatc atgctgggaa agaataaaaa ataattggga
    15241 atcacaaaaa agaatgctag gataatagca agagcaaatg cgcagagatg tgaataaatg
    15301 agaacgtttt ctcgtttgcg tacaacaaca aattttaagg caggaaagag gatttcaagt
    15361 ggaaaaaaaa tacatcatgt ctatagatca gggaacaacc agcacacgtg caatcatctg
    15421 ggataagaaa ggcacaatca tctcttcttc gcaaagagaa ttgatgcaat attatcctga
    15481 accgggttgg gtggaacatg acgcgaatga aatttggatc agtgtccagt cggtcatagc
    15541 ggatgccttg atcagaggag ctattcgtcc ggaggagatt gcggcaatag gtgtaacgaa
    15601 tcagcgcgag acgacggttg tgtgggaccg cgttaccgga atgccgatcc accatgcaat
    15661 cgtttggcaa tcccgccaaa cttcggagat cgccgataaa ctgaagcagg aaggccatag
    15721 tgacttcatc catgataaaa ccggactgat catcgattct tatttttcgg ccacaaaaat
    15781 aaaatggttg ctcgatcata tcccgaatgc gcgtgagcgt gcggaaaagg gggaactgct
    15841 cttcgggac
//
LOCUS       DAOMUJ010000003        15147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_823_length_15147_cov_3.380997, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000003 DAOMUJ010000000
VERSION     DAOMUJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 15147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15147
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_15147_cov_3.380997"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLT73_00185"
     CDS             <1..580
                     /locus_tag="PLT73_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HRM18829.1"
                     /translation="YQGEEIGMTNPDYDDISVYRDIESHNAYRELKLAGLTHQEAMRI
                     IKQKSRDNSRTPMQWDSSRHAGFTTGEPWLQVASNYPEVNVEKELAEGKIFRYYQELI
                     RIRKEHAVIADGSYEGILLDDSEVFAYIRENEEEKVLVLNHFYAGEYTISVPEEWASK
                     ASSFLVGNYGERQLASSFTLNPYETIAFRIIK"
     gene            complement(897..2405)
                     /locus_tag="PLT73_00190"
     CDS             complement(897..2405)
                     /locus_tag="PLT73_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRM18830.1"
                     /translation="MKEISLNDTVYNLVKQYPEIRELIISLGFEPLRSDKMLNTAGRI
                     MTIGKALKRNDVPEELFVETLAKNGFSVKELAISAVTVAPSESEEPSAPTGGIARMGS
                     TEFQERQDILQSLILRLHRGEDWDAVQADFKKHFTGVSAYEISVMEQGLMGKGIDAED
                     IQRLCNVHASLFGGSIEAVYTTSTEQQHAGHPIQVMKLENLAIENTLEKISKLLAVYL
                     ETKEPEIKKGIQNQLNLLGEFDKHYARKEYAIFPIMENKGITAPPKVMWGVDDEIRSM
                     FKEFRTLLQNEDFDAIPKAFSDLKYEMTEMITKEEDILLPMIIDIFNEDHWLQIAQES
                     DEIGYCIVKPEAKWVPERTLPENVSHETFITEENAYINFQTGHLTPHQLEKMLNNIPL
                     ELTFVDADNIVRYYNDNGEEKFFKRTSSAIGRDVLNCHPPKSLPIVTKLLADLKSGAK
                     ESESMWFRAMGKFILVTYRAVRDDDGSYMGTLEYVQDIQPIIDLDGEKRTLS"
     gene            2589..3719
                     /locus_tag="PLT73_00195"
     CDS             2589..3719
                     /locus_tag="PLT73_00195"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRM18831.1"
                     /translation="MRKKVPNLMVALVVPLLASCSLFGGSDSSSDSSAVSSVSSSVSS
                     STDSGSASSSSSENVEAVTIEAYYPFSASVFTKYLGEGNEYASFTVYPQYIEGNRMQI
                     TSNNGGTQVVTVLELADGELREVFTRPETYFRENMLEKTASGDAANQLDILLKEPLEV
                     GNSWVNASGVPSEITNLTAQIETPMGNFEALEVTTTYEASTTKDYYVKDMGLVKRVTD
                     LGDDMVVSSSLEARNEDVPETQQLRVFYPDSELMGLNATSIALSYTTNDITRTILAEA
                     LKNVPDAPGGSLIGPNVSINSLSLSDDGRVYVDFSQEFVSEMNAGTSAESLILQGVVN
                     TIGEYYGVQEVYLTVAGNPYESGHILMAEGAYFTVDYTKVNE"
     gene            complement(4049..4765)
                     /gene="treR"
                     /locus_tag="PLT73_00200"
     CDS             complement(4049..4765)
                     /gene="treR"
                     /locus_tag="PLT73_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HRM18832.1"
                     /translation="MNKFFDIYMDLRKKIEHAEFETGSLLPSEKTLSDHYHVSRETIR
                     KALALLLEGGYIQKKQGKGSIVLDIRRFNFPVSGLTSFKELQDSQQIPNETIVIKNKL
                     ETLPEFLAENLKVDPGREVISLVRLRKIAGEVVIMDKDYLFHDIVEQIPSEAAENSIY
                     AYLENELGLNIAYAKKEFVVEPATREDHKLMNLKDDTHIVVVKSEVYLEDTRLFQYTE
                     SRHRLDRFRFVEFARRKHSL"
     gene            5130..5618
                     /gene="purE"
                     /locus_tag="PLT73_00205"
     CDS             5130..5618
                     /gene="purE"
                     /locus_tag="PLT73_00205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRM18833.1"
                     /translation="MNTVVSVVMGSTSDWETMKEACIILDEFGIAYEKKVVSAHRTPD
                     LMFTFAEQARAAGTKVIIAGAGGAAHLPGMIAAKTTLPVIGVPVQSRALSGMDSLLSI
                     VQMPGGVPVATVAIGKAGAINAGLLAVQILSIENQDLADRLEKRREDLKQKVLESSED
                     LV"
     gene            5602..6744
                     /gene="purK"
                     /locus_tag="PLT73_00210"
     CDS             5602..6744
                     /gene="purK"
                     /locus_tag="PLT73_00210"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRM18834.1"
                     /translation="MKILYKILKPNDVIGIIGGGQLGRMLAQSAKRMGFTVGILDPGE
                     NCPAAQVADWHIQAAYADAVALRELAEKSDVVTFEFENIDAVALQEVETLSSIPQGSG
                     ILTITQDRIKEKEFLQSAGVSIAQFAVVENAEQLDAAIAKIGYPSVLKTTRFGYDGKG
                     QVVLKSEADKAEAMKLGTDSICVLEAWVPFTKELSVMIVRNQKGEATVFPVSENIHIN
                     NILHESIVPARVTPAVAEKANKAAHQIADALQLVGTLGIEMFLAADDTIFINELAPRP
                     HNSGHYTIEAMNVSQFDAHIQAVAGLPMPTARQLSPAIMVNILGQHMEGTIALREEKA
                     DWSFHYYGKAESRTDRKMGHITILTDDQEATLTEIDNTRIWNGGTI"
     gene            6746..>7394
                     /locus_tag="PLT73_00215"
     CDS             6746..>7394
                     /locus_tag="PLT73_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM18835.1"
                     /translation="MLTRYTRPEMAEVWSEYNRYKCWLEVEILADEAWAALGEIPAED
                     VAKIRANATFDIDRILEIEKETRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTAY
                     GYQLKQANDILRKDLQNMLEIIGNKAKEHKKTVCMGRTHGVHAEPTTFGLKLATMYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFVEEYVCEKLGIRPQEISTQVLPR"
     assembly_gap    7395..7404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7405..8056
                     /locus_tag="PLT73_00220"
     CDS             <7405..8056
                     /locus_tag="PLT73_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM18836.1"
                     /translation="DLHAEYMATIALIATSIERFATEIRGLQKSETREVEEFFAKGQK
                     GSSAMPHKRNPIGSENMVGLARVIRGYMVTAYENVGLWHERDISHSSAERIILPDATT
                     LLNYQLNRFGNIIKNLTVFPENMLRNMNATFGLIYSQRVLLKLIDKGMSREEAYDLVQ
                     PKTALSWDNQTDFRPLVEADEAIMAKLTKEDIEDAFNYNYHLSHVDEVFARLGLGD"
     gene            8249..8959
                     /locus_tag="PLT73_00225"
     CDS             8249..8959
                     /locus_tag="PLT73_00225"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRM18837.1"
                     /translation="MERKELLYTGKAKKMYATDQENVLWAEYLNQATALNGIKKDTIE
                     GKGRLNNQITGRIFEYLAEKGIASHYIQELSETEQSVKRVNMFPLEVVVRNVAAGSFA
                     KRFGMEEGIDLPFPVLEFYYKDDALNDPFINDAHVQVLGAATEEEVAEIKQQALKINA
                     ALIEMFKAIGIRLIDFKIEFGKTAEGEIILADEISPETCRLWDIETNEHLDKDIYRRD
                     LGDLIPVYVEVLDRLNKM"
     gene            9030..9281
                     /gene="purS"
                     /locus_tag="PLT73_00230"
     CDS             9030..9281
                     /gene="purS"
                     /locus_tag="PLT73_00230"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRM18838.1"
                     /translation="MYKVTVYVTYKDSVLDPQGEAVKGAVHRMGYPTIEDIRIGKYFE
                     IQVSKDEENVEQVIEEICDKLLANVVMETYRYEIQEVEA"
     gene            9281..9961
                     /gene="purQ"
                     /locus_tag="PLT73_00235"
     CDS             9281..9961
                     /gene="purQ"
                     /locus_tag="PLT73_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRM18839.1"
                     /translation="MKFAVIVFPGSNCDLDMYAAIKDILGEDAEYVQHYETSLEGFDA
                     VLLPGGFTYGDYLRTGAIARFANIMSEVVRFAEEGKPVFGTCNGFQILAEAGLLPGAL
                     RRNDTLKFVCKPQDLKVVNADTQFTSLYKEDEIISVPIAHGEGNYFCDAETLADLKAN
                     NQIVFTYANGNPNGSIEDIAGIINKKGNVLGMMPHPERAVEALLGSADGLRLCQSMVE
                     NYKKELNK"
     gene            9961..12189
                     /gene="purL"
                     /locus_tag="PLT73_00240"
     CDS             9961..12189
                     /gene="purL"
                     /locus_tag="PLT73_00240"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRM18840.1"
                     /translation="MAFLEPTPEQVKESKIYREWGLTDEEYGTICDKILHRLPNYTET
                     GLFAVMWSEHCSYKNSKPVLRKFPTTGPQVLQGPGEGAGIVDIGDGQAVVFKAESHNH
                     PSAVEPYEGAATGVGGIIRDIFSMGARPIAILDSLRFGELDNERTKHIFEEVVAGISG
                     YGNCIGIPTVGGETVFDPCYKGNPLVNAMCVGLIDQKDMQKGQAAGVGNSIMYVGAKT
                     GRDGIHGATFASEEFKEEEEAQRSAVQVGDPFMEKLLMEACLECIYDYSDALIGIQDM
                     GAAGLVSSSSEMASKAGSGLLLNLDDVPQRETEMTPYEMMLSESQERMLLCIKKGEEQ
                     RIVDLFKKYELDAVVIGEVTDDGMYRLSHAGKVVAELPVDALAEDAPVYYKPTAVPAR
                     IAAFAAMEDYKPVFTSAQDTLVALLQQATLASKKSVYDTYDSMVRTSTVVGPGSDAAV
                     VRVRGTKKAIAMTTDCNGRYLYLNPEIGGQIAVAEAARNIVASGGKPLAITDCLNYGN
                     PDKPEIFWELSTSADGISEACRQLDTPVISGNVSLYNETDGVAVYPTPMIGMVGLIED
                     LAHITTQAFKAAGDHIILIGETKADFNGSELQKMELGKIEGKLMDFDLAVEKENQANV
                     LKAIKAGLIASAHDLSEGGLAVGLMESVFDTGLGFDVTVAMDKTLLFSETQSRFILTV
                     KPENVAAVEAIFGSAAAQIGTVTAAAVAKIAAANETITLDIKEVQTKWEEAIPCLLKQ
                     KA"
     gene            12165..13625
                     /gene="purF"
                     /locus_tag="PLT73_00245"
     CDS             12165..13625
                     /gene="purF"
                     /locus_tag="PLT73_00245"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM18841.1"
                     /translation="MLAETKSLNEECGVFGIWGDDNASQLTYFGLHSLQHRGQEGAGI
                     VSCDEGKLLVHRNLGLISEVFKDEADLQRLTGNRAIGHVRYSTSGQNSIENVQPFLYH
                     FYDMDIAICHNGNLVNAKSLRRNLEENGAIFHSTSDTEVLMHLIRRSQKDSLIEKIKE
                     SLNQVKGGFTYILLTEEKMFGAVDPNSFRPLVIGQLPNGAYVMASETCAIDTIGADFV
                     RNVNAGELAIIDDNGLTIEKYTDDTTISIAAMEYIYFARPDSNIAGVNVHTARKRMGT
                     RLAIEAPAVTADIVIGVPNSSLSAASGFAEESGTPYEMGLIKNQYVGRTFIQPTQELR
                     ELGVKMKLSAVKGVVQGKSVVMVDDSIVRGTTSKRIVTLLKEAGAKEVHVRISSPPLI
                     YPSFYGIDISKSAELIAAKMTVDEICAYIGADSLAFLSQEGLIDSIGLKFDMPYSGLC
                     MDSFNGDYPTGLYDYEADYVNNMTAIQKENLKGGQA"
     gene            13625..14674
                     /gene="purM"
                     /locus_tag="PLT73_00250"
     CDS             13625..14674
                     /gene="purM"
                     /locus_tag="PLT73_00250"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM18842.1"
                     /translation="MENAYAKAGVDVTAGYETVSRIKKHIDRTKRPGVFGEVGGFGGN
                     FDLSELNYKKPVLVSGTDGVGTKLMLAIEAGKFDTIGIDCVAMCVNDVVAQGAEPLYF
                     LDYVAVGKNRPEKLEQIVAGVAEGCVQAGAALIGGETAEMPDMYDPEDFDLAGFTVGI
                     AEKDALLTPALIKEGDVLVGLPSSGIHSNGYSLVRKIFFKDNDYKLADKVDGIEDQEL
                     GDVLLTPTRIYVNALLPLIKKQLLHGVAHITGGGFVENVPRMLPDNVQAEIQLGSWPV
                     LPIFQVMEKIGKIPALEMYEIFNMGIGMVLAVAPENLTEVLGVLTENDEKAYVIGSVA
                     AKTSEKEVILKEAQA"
     gene            14671..>15147
                     /gene="purN"
                     /locus_tag="PLT73_00255"
     CDS             14671..>15147
                     /gene="purN"
                     /locus_tag="PLT73_00255"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRM18843.1"
                     /translation="MRIAVFASGNGSNFQAIAEAIASGEVDATIAFLFCDKPKAYAIE
                     RAKQHNIPVISFTPKGFADRVAYENEILKHLAEEKVDLVVLAGYMRIVGPTLLSAYAD
                     RIVNIHPSLLPDFPGRHGIQDAFEAGVLETGVTVHFVDEGVDTGPIIAQEKVAILPE"
BASE COUNT         4080 a         3432 c         3894 g         3731 t
ORIGIN      
        1 ctatcagggc gaggaaatcg gcatgacgaa tccggattat gacgacatca gcgtttatcg
       61 cgatatcgaa tcgcacaatg cctaccgcga gctgaaactt gccggattga cgcatcagga
      121 ggcaatgcgg atcatcaaac aaaaatcccg cgacaacagc cggacgccga tgcaatggga
      181 tagcagccgg cacgcaggct tcacgaccgg cgaaccttgg ctgcaggtgg cgtccaacta
      241 cccggaagtg aacgtcgaga aggagctcgc ggaaggcaaa attttccgtt actaccagga
      301 gttgatccgt atccgcaaag agcatgcagt cattgcggat ggttcctacg agggtatcct
      361 gttggacgat tcggaagtgt tcgcctatat ccgggagaac gaggaagaaa aagtgttggt
      421 tctgaatcat ttctatgccg gtgagtacac aatcagcgta ccggaagaat gggcaagcaa
      481 ggccagcagt ttcttggtcg gcaattatgg ggaaaggcag cttgcgtctt ccttcacgct
      541 gaacccgtac gaaaccatcg ctttcaggat aatcaaataa gcacaaaaga aatggtcgtc
      601 tcactagaga tggccatttt ttggtgccct gctccggtaa gacgcatgct cacaggagaa
      661 aaccggcaac tatcgatgtg aacgccgacg aagcggcgct cataggagct ggcaagaaaa
      721 atgttggccg gcatccggcg aggcctccta ttaccggagc agaacggtga taggagacga
      781 tgaaggatga ttacacagaa agttaccggc taacgccaaa aataccgggc ggcctcaaaa
      841 atggggccgc tcggtatttt tgttgtttgg caatcgctcc tgcacttcac agtagttcag
      901 gaaagggtgc gtttttcgcc gtcaagatcg atgatcggct ggatgtcctg cacatattca
      961 agggttccca tgtagctgcc gtcgtcatcc ctgacggcgc gataggttac caggatgaat
     1021 ttgcccatcg cgcggaacca catcgattcg ctttctttcg cgcctgattt caaatcagcg
     1081 agcagttttg tgacaatcgg caggctcttc ggtggatggc aattcagcac atctcggccg
     1141 attgcactgc ttgtgcgctt gaagaacttt tcttcgccgt tgtcgttgta gtagcgcacg
     1201 atattgtcgg catcgacgaa agtcaattcc aaagggatat tattcagcat cttttccaac
     1261 tggtgcggcg tcagatgtcc ggtttggaaa tttatgtatg cattttcttc agttataaat
     1321 gtttcatgtg aaacattttc tgggagggtc cgttccggca cccattttgc ttccggtttt
     1381 acgatgcagt agccgatttc atcagactcc tgggcgatct gtagccaatg gtcctcgttg
     1441 aagatgtcga taatcatcgg caagaggata tcttcctctt ttgtgatcat ttccgtcatt
     1501 tcgtatttca ggtcggagaa tgctttcggg atcgcatcga agtcctcgtt ctgcaacagt
     1561 gttcggaatt ccttgaacat gctgcgaatt tcatcatcga caccccacat gactttcggt
     1621 ggggctgtga tgcctttgtt ttccataatc gggaagatgg cgtattcttt gcgggcatag
     1681 tgcttatcga attcgccgag caaattcaat tggttttgga tacctttttt gatttccggc
     1741 tcttttgttt caagatagac agccagtaat ttgctgattt tttccagcgt attttcgatg
     1801 gctaagtttt cgagtttcat aacttggatt ggatggccgg catgctgctg ttctgttgaa
     1861 gtggtgtaga ccgcttcgat ggatccgccg aagagcgagg cgtgcacgtt gcaaaggcgc
     1921 tggatgtctt cggcgtcaat ccctttgccc atcaggcctt gctccatgac ggaaatttcg
     1981 taggcactga cgcccgtgaa atgtttcttg aagtcggctt gcaccgcatc ccagtcctcc
     2041 cctctatgca aacgcaggat gaggctctgc agaatgtctt ggcgctcctg gaattccgtt
     2101 gaacccatgc gggcaattcc accggttgga gcagagggct cttcggattc gcttggggca
     2161 accgttacgg ctgatattgc caattccttc acgctgaatc cgtttttcgc cagcgtttca
     2221 acgaatagtt cctccggcac atcattgcgc ttgagggctt tccctatagt catgatgcgg
     2281 ccggcggtgt tcagcatttt gtcgctgcgc aatggttcga agcccaggct gatgatgagc
     2341 tccctgattt cagggtactg ctttaccaga ttgtagacgg tatcgttcaa actgatttcc
     2401 ttcatatttt ttccacctct atttaaaatt tgtgaaatgt ttcttttagt ttcgtgctgg
     2461 cttcattata cttgaaagga gctgctcagt gccacagcat tcgtgcacgg cagcaatagt
     2521 tagggtaatg ccttagaaag cggttgtgat ataatgaaga caactaaggc agaaggtggg
     2581 aaaaaaatat gagaaagaaa gttccgaatc tgatggtagc gttagtggtg cccttattag
     2641 cttcttgcag tctctttgga ggatccgatt cctcatccga ttcttcggct gtcagcagtg
     2701 tatcttcttc cgtgtccagt tcaacagaca gcggctcggc ttcctcgtcc tcttccgaaa
     2761 atgtagaagc agtgacaatc gaagcttatt atccgttcag cgcatccgta ttcactaaat
     2821 atttgggcga ggggaatgag tatgcttcct tcacggtcta tccgcaatac atcgagggta
     2881 accggatgca aatcacttcc aacaacggtg gcacgcaggt tgtgacggta ttggaactgg
     2941 ctgacgggga actcagagaa gtattcacgc gtccggaaac ctacttcagg gaaaatatgc
     3001 ttgaaaaaac agcaagcggc gatgcggcaa accagttgga tattctgttg aaggagcctt
     3061 tggaagtcgg gaacagttgg gtgaatgcga gcggagtccc ttccgaaatc acgaatctga
     3121 cggcacagat cgaaacacca atgggtaatt ttgaagcgct tgaagtgacg acgacttatg
     3181 aagcttcgac taccaaggat tactatgtga aagatatggg tttggtgaaa cgggtgacgg
     3241 accttgggga tgacatggtt gtctcttcca gtctggaagc ccgcaacgag gacgttccag
     3301 aaacgcaaca gttgcgcgtt ttttatccgg acagcgagct gatgggattg aatgcaacaa
     3361 gcatcgcttt atcttatacg accaatgata ttacccgcac cattctggcg gaagcgctga
     3421 agaatgttcc ggatgctcca gggggaagtc tgattgggcc gaatgtctcc atcaacagct
     3481 tgtccttgag tgacgatggg cgtgtctatg tggacttctc gcaggaattt gtttctgaaa
     3541 tgaatgccgg aacatcagcg gaatcgttga ttttgcaggg agtcgtcaat acgatcgggg
     3601 aatattacgg agtccaggaa gtctacctta cggtggccgg aaacccttac gaatcagggc
     3661 atatcctgat ggctgaaggt gcctatttta ctgttgatta tacgaaagtc aatgaatgat
     3721 cagtccttac ataagcaaaa aaagatgaag ccggcttggc ttcatctttt ttctaatttg
     3781 tagcatgtta ttaactaaaa agcgcatcac catgggtgtt ttataggttt gcacaacgtt
     3841 ttatgcatct aaaatgatgg ctagcggtcc aagcctgcct ctcggaaatt agataaatct
     3901 gacccattgc gctctgcgat gctcatttgc atgggagtct tagggattca tcccttagac
     3961 tcccagtaca aggtgaccgc agggaacgtt gcgaggccag atttcctaaa tttctttcga
     4021 ggcaaagtgg gcttgtcccg cttttctttt ataggctgtg ttttctgcgg gcgaattcga
     4081 cgaagcggaa gcgatccaaa cgatggcgag actcggtgta ctggaaaaga cgcgtatcct
     4141 ccagatagac ttcgctcttt acgacaacaa tatgcgtgtc atcttttaaa ttcatcagtt
     4201 tgtgatcttc ccgtgtcgca ggctccacaa cgaattcctt tttggcataa gcgatattca
     4261 gaccaagttc attctccaaa tacgcataaa tggaattttc agcagcttcg gaagggattt
     4321 gttcgacgat atcatggaag agatagtctt tgtccatgat gacaacttcc ccggcaatct
     4381 tgcgaagccg gacaagggaa attacttcgc ggcctggatc taccttcaaa ttttctgcaa
     4441 ggaactccgg aagcgtctct agtttatttt tgatgacgat tgtttcattt gggatttgtt
     4501 gggagtcttg aagctcttta aaactggtaa gcccggaaac agggaaattg aagcgtctga
     4561 tgtcaagtac aatggaacct ttaccttgtt tcttttggat atagccacct tccagcaaca
     4621 gggccaaagc ctttctgatt gtttcccgcg aaacatgata gtggtccgaa agtgtttttt
     4681 cgctgggcaa cagagatcct gtttcgaatt cagcatgttc gatttttttt ctcaaatcca
     4741 tatagatatc aaagaattta ttcataatca tcactccttc tgtaagtgga tgggtttatt
     4801 ggaaataaaa taaataagtt gaaataatcg tgtttaataa tattaataat attattcccg
     4861 tttattctat attataacca cggaaataaa aagagaatca gcattatttt aatttgtaag
     4921 ggtttgcaca tcattatttt atcatattgt tgccatttat ggtgaaaaat tttcactttt
     4981 ttaatgtgaa atgaggtcat tatttcttct cttttgaaaa aaaatagatt atttccgaac
     5041 gaacggtcct tcagtatcta aaattgtttg gaataattac aggtgatgtg ctatattaaa
     5101 aatgaaaaat cgataatggg ggaaaatgga tgaatacggt tgtttctgtc gtaatgggta
     5161 gtacttctga ctgggaaact atgaaagaag cttgcataat actggatgaa ttcggtatcg
     5221 cttatgaaaa gaaagtcgtt tcggcacacc ggacgcctga tctgatgttc acttttgcgg
     5281 aacaagcacg ggctgcggga acgaaagtca tcattgccgg cgcgggcggc gcagcgcacc
     5341 ttccgggcat gattgccgcg aaaacgacgc ttccggtcat cggtgttccg gtccaatcgc
     5401 gcgctttgag cggaatggat tctttgttgt ccatcgtcca gatgcccggt ggtgttccgg
     5461 ttgcgacggt agcgatcggc aaagcagggg caatcaatgc cggtctgctg gccgtgcaga
     5521 tcctttcaat cgaaaatcag gatttggccg atcgattgga aaagagacgg gaagatttaa
     5581 aacaaaaagt attggaaagt agtgaagatc ttgtataaaa tactgaaacc gaacgacgtg
     5641 atcggcatca tcggcggggg acaattagga cgtatgctgg cacagtccgc aaagaggatg
     5701 ggattcaccg tcggaatttt ggatccggga gaaaattgcc cggcagccca agttgcggat
     5761 tggcacatcc aggcggcata cgctgacgca gtggctttgc gcgaattggc cgaaaaatcc
     5821 gatgtcgtga cattcgaatt tgagaacatc gatgcagtag cgctgcagga agtggaaaca
     5881 ctgtccagca ttccgcaagg cagcggaatt ttgacgatca cgcaggaccg catcaaggag
     5941 aaggaattcc ttcaatccgc cggtgtatcg attgcgcaat tcgcggtagt ggaaaatgcg
     6001 gaacaattgg atgcggccat cgctaaaatc ggttatccga gtgttttaaa aacgacccgt
     6061 ttcgggtatg atggtaaggg acaggttgtc ctgaaatcgg aagcggacaa agcggaagcc
     6121 atgaaactgg gaacggacag catctgcgtt ttggaggctt gggttccgtt cacgaaagaa
     6181 ctttcggtca tgatcgtacg caatcaaaaa ggtgaagcga cggtgttccc ggtctccgaa
     6241 aatatccaca tcaacaatat tctgcacgaa tcgattgtgc cggcccgcgt cacgcctgcg
     6301 gtagccgaaa aagccaacaa ggcggcccac caaatcgccg acgcgctgca gttggtaggt
     6361 acgctgggca tcgaaatgtt cctggctgcg gatgatacga tcttcatcaa tgagttggcg
     6421 ccgcgtccgc ataattctgg ccattacaca atcgaggcaa tgaacgtttc ccaatttgat
     6481 gcccatatcc aagcagtcgc cggattgccg atgccgacag cgcgtcaatt gtcgcctgcc
     6541 atcatggtca atatcctggg acagcatatg gaaggcacaa tcgcgctgcg ggaagaaaaa
     6601 gcggattggt ctttccatta ctacggcaaa gcggaatcgc gcacagacag aaaaatgggt
     6661 catatcacca ttttgacaga tgatcaagaa gcaaccttaa cagaaataga caatacccga
     6721 atctggaacg gaggaacaat ttaaaatgct tacacgttat acacgccctg agatggccga
     6781 ggtttggtcc gaatacaatc gttacaaatg ctggctggag gtcgaaatcc tggctgatga
     6841 agcttgggca gccctaggtg agatccctgc ggaagacgtt gctaaaatcc gcgcgaatgc
     6901 aacttttgat atcgaccgta tccttgaaat cgaaaaagag accagacatg atgtagtggc
     6961 gttcacacgc gccgtttccg aatctttggg agaagaaaga aaatgggtcc actatggcct
     7021 tacgagtacc gatgtggtcg atactgccta cggttaccaa ttaaagcaag ctaatgatat
     7081 cctgcgcaag gatctgcaga atatgctgga aatcatcggc aacaaggcaa aagaacacaa
     7141 aaagactgtc tgcatgggtc gtacgcatgg tgtacatgca gaacctacga cattcggctt
     7201 gaaactggct acgatgtatt cagaaatgaa acgcaacatc gagcgttttg aacacgcagc
     7261 aaaaggtgtt gaagccggaa aaatcagcgg tgcagtaggc actttcgcaa acatcccgcc
     7321 attcgtggag gagtatgtct gtgaaaaatt aggtataaga ccgcaagaga tttcgacgca
     7381 ggttcttccg cgcgnnnnnn nnnncgatct gcatgccgaa tatatggcaa cgatcgcttt
     7441 gatcgcgaca agcatcgagc gtttcgcaac tgaaatacgt ggactgcaaa aatcggaaac
     7501 ccgcgaagtg gaagaattct tcgcgaaagg ccaaaaaggt tcttccgcta tgccgcacaa
     7561 gcgcaacccg atcggttccg aaaatatggt gggccttgcc cgcgtcatcc gcggttacat
     7621 ggtcactgct tacgagaatg taggcttatg gcatgaacgc gacatttccc actcttctgc
     7681 ggaacgcatc atccttcctg acgcaacgac attgttgaac tatcagttga accgttttgg
     7741 gaacatcatc aagaacttga ctgtgttccc tgagaatatg ctccgcaaca tgaatgccac
     7801 gttcggattg atctatagcc aacgcgttct gttgaaattg atcgacaaag gtatgagccg
     7861 tgaagaagct tatgacttgg tacagccgaa gactgccctt tcatgggata atcagaccga
     7921 tttccgtcca ttggtggaag cggacgaagc gatcatggca aaattaacga aagaagacat
     7981 cgaggatgcc ttcaactata actatcactt gagtcacgtc gacgaagtat tcgcaagact
     8041 tggtttagga gactaatgga aaggctggga ctacggttcc ggcctttttc tgttcatcgg
     8101 atgactgaaa ggcactgagg aaaaaccggg atttatttca tttttcatga aaatgccatt
     8161 gacaagtaga gggcagatga tacaatataa acgaatgaat aaggcgtttg tataaattaa
     8221 tgttcggttt tggaaaggga cggggaagat ggaaagaaaa gaattgcttt atactgggaa
     8281 agctaaaaag atgtatgcta cagatcagga gaatgttctt tgggccgagt acctaaatca
     8341 agctacggca ttaaacggca taaaaaagga tactatcgag gggaaaggca gactcaataa
     8401 ccagatcacc ggccgcatct tcgagtatct ggctgaaaag ggtatcgcga gccattacat
     8461 ccaagaacta tcagaaaccg aacaatcagt gaaaagagtc aatatgttcc cattggaagt
     8521 ggttgtccgc aatgtagccg ctggaagctt cgcgaaacgt ttcggcatgg aggaaggcat
     8581 cgacttgccg ttccctgttt tggaattcta ctacaaagac gacgcgctca atgatccttt
     8641 catcaatgat gcccatgtac aagtccttgg agcagcgact gaagaagaag ttgccgaaat
     8701 caagcagcaa gcgctgaaga tcaatgcagc cttgatcgag atgttcaagg caatcggcat
     8761 ccgcctgatc gatttcaaaa tcgaattcgg caagacagct gagggagaaa tcatcctggc
     8821 tgatgaaatc tcacctgaga catgccgcct ttgggatatc gaaacgaacg aacacttgga
     8881 taaagacatc taccgccgtg acttgggaga tctgatccct gtctacgtgg aagtgctgga
     8941 ccgcctgaac aaaatgtaag ccatccgaat gcaactaacg aataaacaaa aaacaaaaaa
     9001 ataaaaaaac aaaagcgagg aatttggaca tgtataaagt aaccgtgtat gtaacgtata
     9061 aagactcagt attggatccg caaggggaag ccgtgaaggg tgccgtccac cgtatgggct
     9121 acccgacgat cgaagatatc cgcatcggga aatatttcga aatccaagtt tccaaagatg
     9181 aagagaacgt tgaacaagtc atcgaagaga tctgcgacaa attgctggcg aacgtggtca
     9241 tggaaactta ccgttatgaa atccaggagg ttgaagctta atgaaattcg ctgtcatcgt
     9301 ttttcctggc tcgaactgtg atttagatat gtatgcagcc atcaaagata ttcttggaga
     9361 agatgcagag tatgtgcaac attatgaaac aagccttgaa ggtttcgatg ctgtcctatt
     9421 gcccggcgga ttcacttatg gcgactactt gcgtactggt gcgattgccc gctttgcgaa
     9481 catcatgagc gaagtcgtcc gtttcgctga agaaggcaag cctgtattcg gcacttgcaa
     9541 cggattccag attttggctg aagccggctt attgcctggt gctttgcgcc gcaatgatac
     9601 tttgaagttc gtctgcaaac cgcaggatct caaagtcgtg aatgctgata cgcaattcac
     9661 ttccctctat aaagaagacg aaatcatctc tgttccgatc gcgcacggtg aaggaaacta
     9721 cttctgcgat gcagagactt tggctgacct gaaagccaac aatcaaatcg tattcactta
     9781 tgctaatggt aacccgaacg gcagcatcga agacatcgcc ggcatcatca acaaaaaagg
     9841 caacgtcctc ggcatgatgc ctcacccgga gcgtgctgtc gaagctttgc tgggatcggc
     9901 tgacggcctg cgcctgtgcc aatccatggt tgaaaattat aagaaggagc tgaacaaata
     9961 atggcatttt tagaaccaac gccagaacaa gtaaaagaat ccaaaattta tcgtgaatgg
    10021 ggtctgacag acgaagagta cggcacaatc tgcgataaaa ttttgcatcg tttgccgaat
    10081 tatactgaaa ctggcctgtt tgccgttatg tggagcgaac actgttctta taaaaattca
    10141 aaaccagtcc tgagaaaatt ccctactacc ggcccgcaag tgttgcaagg acctggcgaa
    10201 ggcgctggta tcgtggatat cggcgacggc caagctgtcg ttttcaaagc ggaaagccac
    10261 aaccacccat cagctgtcga gccttatgaa ggtgcagcta ccggtgtcgg cggaatcatc
    10321 cgtgacatct tcagcatggg cgcacgccca attgcgatcc ttgactcttt gcgtttcggc
    10381 gaattggaca atgagcgcac aaaacacatc ttcgaagaag tcgttgccgg tatcagcggt
    10441 tacggcaact gtatcggtat tccgactgtc ggcggcgaaa cggtcttcga cccttgctac
    10501 aaaggcaatc cgttggtcaa cgcaatgtgc gtcggtttga tcgatcaaaa agatatgcaa
    10561 aaaggccaag ctgccggagt cggcaactcg atcatgtacg tgggcgcaaa aacaggccgc
    10621 gatggcatcc atggcgccac tttcgcttcc gaagaattca aggaagagga agaagcgcaa
    10681 cgttcagctg tccaagtggg cgatccattc atggaaaaat tattgatgga agcttgtttg
    10741 gaatgcattt atgactactc cgacgctttg atcggtatcc aggacatggg tgctgccggt
    10801 ttagtctctt ccagctcgga aatggcttca aaagcaggca gcggcttgct tttgaacctt
    10861 gacgatgttc cgcagcgcga aacagaaatg acaccttacg aaatgatgct ttccgagtca
    10921 caggagcgta tgctgttgtg catcaagaag ggcgaagagc aacgcatcgt cgacctgttc
    10981 aaaaaatatg aattggatgc agtcgtgatc ggtgaagtta ccgatgatgg catgtaccgt
    11041 ctatcccatg caggcaaagt ggtcgctgaa cttccagttg atgctttggc tgaagatgca
    11101 ccggtctact acaaaccgac tgcagtgcct gcccgcatcg ctgcattcgc agctatggaa
    11161 gactataagc cagtcttcac atcagcgcag gataccttag tggctttatt gcaacaagcg
    11221 acgttggctt ccaagaagag cgtctatgat acctatgatt ccatggtccg caccagcacg
    11281 gttgtaggcc caggaagcga tgctgcagtt gtccgcgtcc gcggcaccaa aaaagcgatc
    11341 gcaatgacaa cggactgcaa cggccgttac ctgtatttga acccggaaat cggcggacag
    11401 atcgctgtcg ctgaagctgc ccgcaatatc gttgccagcg gcggaaaacc attggcaatc
    11461 acagactgct tgaactacgg aaatcctgat aaaccagaaa ttttctggga attaagcact
    11521 tccgcggacg gtatctctga agcttgccgc caattggata cgcctgtcat ctcaggtaac
    11581 gtgtcccttt acaatgaaac cgacggcgtg gccgtttatc cgacaccgat gatcggtatg
    11641 gttggtctga tcgaggatct tgcacacatc actacgcaag ctttcaaagc tgcaggcgac
    11701 catatcattc tgatcggcga aaccaaagca gatttcaacg gttccgaact gcaaaaaatg
    11761 gaattgggca aaatagaagg caaactgatg gactttgact tggctgtcga aaaagaaaac
    11821 caagcgaacg tcctgaaagc aataaaagcg ggtctgatcg caagcgcgca tgacctatca
    11881 gaaggcggct tggctgtcgg cttgatggag agcgtctttg acaccggtct aggcttcgat
    11941 gtgacggttg ctatggataa aacattgctg ttcagcgaaa cgcaatcacg cttcatcctg
    12001 accgtaaaac ctgaaaatgt tgccgctgtg gaagcgatct tcggatcagc tgctgcgcaa
    12061 atcggaaccg tgactgctgc cgctgtcgca aaaatcgctg ccgcgaacga aacaatcact
    12121 ttggatatta aagaagtaca aacgaaatgg gaggaggcta ttccatgctt gctgaaacaa
    12181 aaagcttaaa cgaagaatgt ggcgtattcg gcatttgggg agacgacaat gccagccagt
    12241 tgacttactt cggtttgcac agcttgcagc accgtggaca agagggtgca gggatagttt
    12301 catgtgatga aggaaaactg ttggttcacc gtaatcttgg tctgatcagc gaagtgttca
    12361 aggacgaagc ggatctgcaa agattgacag ggaaccgtgc catcgggcat gtgcgttact
    12421 cgacttccgg acagaacagc atcgagaatg tgcagccgtt tttgtaccat ttctatgata
    12481 tggacatcgc catttgccac aacggcaact tggtgaatgc caaatcattg cgacgcaatc
    12541 ttgaggaaaa cggcgccatc ttccattcga cttcggatac ggaagttctg atgcatctga
    12601 tcagacgcag ccagaaagac agcctgatcg aaaagatcaa agagagcctg aatcaggtta
    12661 aaggcggctt cacttatatc cttttgaccg aagagaaaat gttcggtgcc gtcgatccga
    12721 attcattccg tccgcttgtc atcggccaat tgccgaatgg cgcgtatgtg atggccagcg
    12781 aaacgtgtgc catcgatacg atcggcgcgg attttgtccg caacgtcaac gccggcgaac
    12841 tggcgatcat tgacgacaac ggcttgacga tcgaaaaata taccgacgat acaaccatct
    12901 ccatcgctgc gatggagtat atctacttcg cgcgtccgga ttccaatatt gccggcgtga
    12961 atgtgcatac tgcacgcaag cggatgggaa cgcgtttggc catcgaagca cctgcagtga
    13021 cagccgatat cgttatcggt gtgcccaact cttccttgtc cgcagcgagc ggatttgcgg
    13081 aagaaagcgg aacgccttac gaaatgggcc tgatcaagaa ccaatatgtc ggacgtacgt
    13141 tcatccaacc gacgcaggaa ctgcgcgaat tgggcgtaaa aatgaagttg tccgccgtaa
    13201 aaggggttgt ccaaggcaag agcgtcgtca tggtggatga ttccatcgtg cgcgggacga
    13261 ccagcaaacg catcgtgacg ttattgaaag aagcgggagc caaagaagtg catgtccgca
    13321 tctcttcccc acctttgatc tacccgagtt tttacggcat cgacatcagc aagtcagctg
    13381 aactgatcgc cgctaaaatg accgttgatg aaatttgtgc atacatcggt gcggacagct
    13441 tggctttcct tagccaagag ggtctgatcg attcaatcgg tttgaaattt gatatgccgt
    13501 attcaggctt atgcatggac agcttcaacg gggattatcc gactgggcta tacgactatg
    13561 aagcggatta cgtgaacaat atgacagcta ttcaaaaaga gaatctaaag ggagggcaag
    13621 cttgatggaa aacgcatatg caaaagcggg tgtggatgtc acagccggtt atgaaacagt
    13681 cagccgcatc aagaaacaca tcgaccgcac aaaacgtcca ggcgtttttg gcgaagtcgg
    13741 cggattcggc ggaaactttg acctttctga attgaattat aaaaaacctg tacttgtatc
    13801 gggtacggat ggtgtcggca cgaagctgat gctggccatc gaagccggaa aattcgatac
    13861 gatcgggatc gattgtgtcg caatgtgcgt caacgatgtc gtagcgcaag gtgcagaacc
    13921 tctttatttc ttggattatg tagctgtcgg taaaaaccgt ccggaaaaat tggagcagat
    13981 cgttgctggc gtagctgaag gctgcgtcca agccggagct gccctgatcg gcggagaaac
    14041 agctgaaatg cccgatatgt acgatccgga agatttcgat ctggccggat ttacggtagg
    14101 gattgcggaa aaagatgctt tgttgacacc tgccttgatc aaagaaggcg atgtgttggt
    14161 tgggttgcct tcatcaggta tccattcgaa cggctattcc ttggtgcgca aaatcttctt
    14221 caaggacaat gactacaaat tggccgacaa agtggatggc atcgaagacc aagaattggg
    14281 cgatgttctg ttgacgccta caagaatcta cgtaaatgct ttattgccgt tgatcaaaaa
    14341 acagctgctg catggtgttg cccacatcac aggcggcggc ttcgtcgaaa acgtgccgcg
    14401 tatgcttccc gataacgtcc aagcggaaat ccaattaggc agctggcctg tcttgccgat
    14461 tttccaagtg atggaaaaaa tcggcaagat tccggctctt gaaatgtacg aaatcttcaa
    14521 catgggcatc ggtatggtgc tggcggtcgc tcctgaaaac ttgactgaag tgctcggcgt
    14581 tctaaccgag aacgatgaaa aagcctacgt gatcggatcc gtggctgcaa aaacatcaga
    14641 aaaagaggtt atcctgaaag aggctcaagc atgaggatag ctgtctttgc ttcaggaaac
    14701 ggctcgaatt ttcaagccat cgccgaagcc atcgcgtcag gcgaagtgga tgcgacgatc
    14761 gctttcctgt tctgcgacaa gccgaaagca tatgcgatcg agcgcgccaa acaacacaat
    14821 atcccggtga tttcctttac gccgaaaggc ttcgcggatc gggtcgccta cgaaaatgaa
    14881 attttgaagc atctggctga agaaaaggtc gacttggttg tcttggccgg ctatatgcgg
    14941 attgtcggac cgactttgct atcggcctac gccgaccgca tcgtcaatat ccacccgtcc
    15001 ttgctgccgg atttcccggg tcggcatggc atccaggatg ccttcgaagc aggcgttctg
    15061 gagaccggcg tcaccgtcca tttcgtcgat gagggggtcg ataccggccc gatcatcgcc
    15121 caagaaaaag tggcgatcct tccggag
//
LOCUS       DAOMUJ010000004        14509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_888_length_14509_cov_2.448526, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000004 DAOMUJ010000000
VERSION     DAOMUJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 14509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14509
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_14509_cov_2.448526"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..733
                     /locus_tag="PLT73_00260"
     CDS             368..733
                     /locus_tag="PLT73_00260"
                     /inference="COORDINATES: protein motif:HMM:NF037826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5613 domain-containing protein"
                     /protein_id="HRM18844.1"
                     /translation="MTFSFKDIEHSKSVVKETSLYTHYHNETALFMYIENYVIFHRVP
                     TFAEFIEAEAYLKSAHQKDNQDFIKFIWPEDCEIPQPIISYLGSNDFSLDVLELYAAP
                     PGTFEPTRIDETPSDVKVE"
     gene            730..1125
                     /locus_tag="PLT73_00265"
     CDS             730..1125
                     /locus_tag="PLT73_00265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM18845.1"
                     /translation="MNADNRSAYLALSARADKEVSEEFAKQKQPYTQAKFYNPAFRPI
                     VALRSDEAVGSIDLYLKEESIEIDNFHVAVDSRGKGIGTALQQFVMDHADGKTVLLVA
                     DAADTAREMYNRQNYTYISFRYELLKVFK"
     gene            complement(1307..1591)
                     /locus_tag="PLT73_00270"
     CDS             complement(1307..1591)
                     /locus_tag="PLT73_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRM18846.1"
                     /translation="MTMNVLAVELSMRLFDSNSLKDKRSVVKSILAKMHQRYNVSIAE
                     VSAQDMLNQAVIGLAVVNSSRVLCQKIVDKIIEEIESNYPVEIYQINEIE"
     gene            complement(1836..2477)
                     /gene="hisIE"
                     /locus_tag="PLT73_00275"
     CDS             complement(1836..2477)
                     /gene="hisIE"
                     /locus_tag="PLT73_00275"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRM18847.1"
                     /translation="MSKYDALVPDFTKGLLTVVLQHFTNKNVLMVGFMNEEAFELTKR
                     DEVVWFFSRSKDRLWKKGESSEHYQFVKDMYLDCDQDALLIMVDPAGPTCHRNTESCF
                     DVPVAFGLDDLEETIRQRAVGHDEKSYTNYLLDKGTDKIAKKFGEEAFEVVIGAKNHD
                     KEEVANETADLLYHLGVLLYDQGVDVADVTKVLIERHQKKNNFKGERKDVDNY"
     gene            complement(2470..3234)
                     /gene="hisF"
                     /locus_tag="PLT73_00280"
     CDS             complement(2470..3234)
                     /gene="hisF"
                     /locus_tag="PLT73_00280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRM18848.1"
                     /translation="MIKKRIIPCLDVKDGTVVKGIQFKGLREIGDPVELAKRYNALGA
                     DELVFLDISATETGHNLMIDVIRKTAQQLFIPMTIGGGIKSTDDISRLLNAGADKVSL
                     NSSALANPQLIKEASDKFGSQCICIAVDAKWEADKEEWYCYTHGGKKRTDIKTLDWVQ
                     QVEALGAGELLVTSMDYDGMKQGFDHRLLNKIQALVSIPVIASGGGGNAQHFADLFKE
                     TDVSAGLAASIFHDEEVSIAEVKECCTENGVDMRNV"
     gene            complement(3231..3902)
                     /locus_tag="PLT73_00285"
     CDS             complement(3231..3902)
                     /locus_tag="PLT73_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRM18849.1"
                     /translation="MIEIWPAIDLIDNKSVRLTEGDYGTKEEMQRTPQEAIAFYAQFE
                     QVKRIHIVDLMGALNQKPEETPFIEQLLQQSALPVEIGGGIRSEETIKHYFEKGASYV
                     IVGTKGLQDLPWLAEMTAKYPGKIYLGLDAKGELVAVNGWTETGRQTIYDVVEATNPM
                     ALGGIIYTDISKDGKMAGPNFELTGKLVELSNHPITASGGVRNIEDIKKLEALGVAAA
                     IVGLA"
     gene            complement(3903..4478)
                     /gene="hisH"
                     /locus_tag="PLT73_00290"
     CDS             complement(3903..4478)
                     /gene="hisH"
                     /locus_tag="PLT73_00290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRM18850.1"
                     /translation="MIAIVDYGLGNIANLTNAIRFLGYETILTHEEAELRKADVIVLP
                     GVGHFKDAIERIDAIGLRELLTELEQTKPFIGICLGMQLLFEHSEEGDVDGLGFLPGE
                     VKYIVSDLPVPHLGWNNLHSSHPGLDKDVYFIHSYKVVTDENVVATADYGQEIVAIVQ
                     KDNVIGIQFHPEKSGDYGLEILNQALQGGWK"
     gene            complement(4475..5068)
                     /gene="hisB"
                     /locus_tag="PLT73_00295"
     CDS             complement(4475..5068)
                     /gene="hisB"
                     /locus_tag="PLT73_00295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRM18851.1"
                     /translation="MERTAVSVSKERITKETKIAITLKRGLEPSVINTGVGFLNHMLD
                     LFAFHGHFVLDVQVDGDTDVDDHHTTEDVGIVLGQLLAELGQDKGAINRYGSAYVPMD
                     ETLARSVIDISGRPYLSFNASFSKEKVGSFDVELVEEFFHGLVINARYTAHIDLIRGG
                     NTHHEIEAIFKAFAQSLRIAMADSDVQRLPSSKGVIE"
     gene            complement(5050..6048)
                     /locus_tag="PLT73_00300"
     CDS             complement(5050..6048)
                     /locus_tag="PLT73_00300"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM18852.1"
                     /translation="MIRINKNESPIRALTNEEIAEIAVSTRFQEYTDDAIDRLTEAYA
                     AFHGEDPALIAFANGSDEWIQKCTILLGDGPILMLEPDFVMYEEYAAQFQREIIKVPC
                     RADYSFDYDQVYAVIEKEKPSYFIVSQPNNPLGGLHPAAFIQKTADLLADSGGYLILD
                     EAYMDFVDNPPARPVGDHVILLRTLSKIYGLAGLRIGIASSTEKTMQLLNSIAHPYPL
                     NTLSLSIAAYLLEHPERLRAFMADQRRLSAKLKKIFNEGVADILSVLPSETNFVFTYG
                     ELAPSLGQWIIDHGYQPRTYEKSGIPCIETAVRYSIATDEQLDALGEIIREWREQL"
     gene            complement(6045..7295)
                     /gene="hisD"
                     /locus_tag="PLT73_00305"
     CDS             complement(6045..7295)
                     /gene="hisD"
                     /locus_tag="PLT73_00305"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM18853.1"
                     /translation="MYTTKTFKEVYKTKNTIDFSKTQAVMEIIQTVQEKGDAALFEYA
                     KRFDGADITELEVPQSVIKEAYDSLDAVLRAALEEARDNITVYQESIKWQQSPAGELY
                     QKIHPLNKVGIYVPGGKASYPSTVLMTAVLANVAGVKETIVVTPPQKTAINQATLAAC
                     YICGVTHVYQVGGAQAVAALAYGTETIPRVDKIVGPGNQYVALAKKLVYGDVGIDSIA
                     GPSEIVVVVDETANNEWVAIDLLAQAEHDELARTFLVCDNEEKLAAIEAELLVQKAKQ
                     SRIEVIEESLKHNHFPILTSGKEENIEVVNLIAGEHVSIQTKDAEDYIDAIETAGAIF
                     IGEYSPEAIGDYVAGPSHVLPTGGNARFANGLSVNDFLRPNSVLNLKKETFQKMAPAG
                     MLIATEESLQAHHDSLAVRMGDEK"
     assembly_gap    7415..7471
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7472..7867)
                     /gene="hisG"
                     /locus_tag="PLT73_00310"
     CDS             complement(<7472..7867)
                     /gene="hisG"
                     /locus_tag="PLT73_00310"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRM18854.1"
                     /translation="MITIALSKGRQLKDFIQYLDEINLTEWSTALKSVSRELVIQTKD
                     TRFILVKGEDVPVYVEEGIADLGITGSDILIEQNCNINNLMDLPFGYCHFAIASKEKK
                     DVYPVVATKYVNFTRQYFDSIKQPVKIIKL"
     gene            complement(7880..8701)
                     /locus_tag="PLT73_00315"
     CDS             complement(7880..8701)
                     /locus_tag="PLT73_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM18855.1"
                     /translation="MMTNESLLAKKQREMGFLHHFHHLGYDLIDLGVVEKFEWTQLSH
                     DDLHLMLNRHKWQSGDKLFALRSDWTNAIVRYRKQYHLRADKIAYSGPVYSLLKERHQ
                     LGVETFTDSIPKQIEVLGDMITFMEKELELSLSVAVVSHNKLLKKILSPRELEDASVR
                     KFICERNQDALKHRLGADHPLIGLMDQAPTEQAGYLKEHYPELTGHLNELAEWEATLK
                     SKNVDYVYADMLALPNQSYYKGIFIQLYGENQTEPVASGGQYTSSSKAFGMAINN"
     gene            8865..9656
                     /gene="hisJ"
                     /locus_tag="PLT73_00320"
     CDS             8865..9656
                     /gene="hisJ"
                     /locus_tag="PLT73_00320"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HRM18856.1"
                     /translation="MLSDRHVHTPYCLHGSSDAMENYVKVAIEAGLESLTFTEHAPLP
                     MADPLPDKDSSMRPEDVDAYLAEVRTLAKKYQGIIEIHAGFELDYLEGKEPETRAFLE
                     KYPETVPHSILSVHFVQLAPEEYFCIDLDCESFTQKGAEIGYETLYTLYEAAVKKALA
                     SPYGELTPKKIGHINLIHKFQKAYSVSDPIDWKLLLDKVKENGYTLDYNFAGIDKPDY
                     GKTYPDKEMMAYAIKIGLSYEMGSDAHASHEVARYFGTAVVETEL"
     assembly_gap    9877..9886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9887..10942)
                     /locus_tag="PLT73_00325"
     CDS             complement(<9887..10942)
                     /locus_tag="PLT73_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM18857.1"
                     /translation="MIRNILLSTMLSLRAHKLRVFLTMVGIIIGIASVVTIAALGEGV
                     KQQSMELANTTEANVVTVKHTIAMSDNSGMGYVEDDFSFSKADMRRISRLDGVQSVVP
                     DYGSNGMAMDSIDVNLDYFGAQAYTMMVPNKSENHILYGRDFQPSDVNRDMIILSHDI
                     LDYGIVVEDPESLIGQAVSINGFMYEIIGIKEPYNWEDSNLLNTGYTWEDAYTSVIPQ
                     AAYNTLTRSKPIKSLKVKVAEGMDNERVTMTVLENLVEAYPEDEGVFEQDRSNEQMME
                     EVNSYINGIMMFLLAITAISLFVGGIGVMNIMYVSVTERRREIGIRRAIGAKPRMILL
                     QFLLEAAFITFLGGIIGL"
     gene            complement(10939..11643)
                     /locus_tag="PLT73_00330"
     CDS             complement(10939..11643)
                     /locus_tag="PLT73_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM18858.1"
                     /translation="MEATKRRSLIQMKDVTKKFGKGELETVVLKEINLTIHEGDFLMI
                     VGKSGSGKTTLMNMIGFLDRLTTGTYSFLGEDVSLLNENKKSSIRNKHFGFIFQQFFL
                     IDSLNVAQNVELPLIYAGEVNKKDRLEAAEKYLSLVGIPEKIKAKTKELSGGQQQRVA
                     IARALINEPLLIMADEPTGALDSDTGIGVMSLLRELNKQGKTIIMVTHDEDLLQYATR
                     IIRMHDGSFVEEGAVR"
     gene            complement(11645..12769)
                     /locus_tag="PLT73_00335"
     CDS             complement(11645..12769)
                     /locus_tag="PLT73_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM18859.1"
                     /translation="MKKKKWLWMGIISVVAVLGVIFLPNYLGKPAEDEPIVEEDPYGI
                     EYYTVPAIEQIFVNGVVTPEQSQEFYQEENLGTRGDLQVENGQVVEAGTLLYSYTNET
                     VQKEVTQLYNEAARLETNRANTAYKQQLAIDRFYEMQEEERAQTLDELIMDFNLYDLD
                     AQIKEIYNNILSLEESVYTEVTAPFTGKVVIPEEKKADVPVLTLISENFYVAGTVNEK
                     DLEKVTIGQVADITVVSSNFTTVGKVSFVDTTPANTVSDPYTQGSSMSSYPVKLSFDS
                     LERIVNGYHVQATINLEENQVVVPKVAIRTDGDVTYVLVNDFGTVTRRVIQTTEEAAE
                     GIVVTSGLEAQDEIIVSSEADVQEGDLLDPTSMEVDLEEE"
     gene            13000..13575
                     /locus_tag="PLT73_00340"
     CDS             13000..13575
                     /locus_tag="PLT73_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HRM18860.1"
                     /translation="MKLKPELEDYQQQLLNLTFIRFDELPDFGLYSDQVIAIIEKQLS
                     FLNATQEERIITPAMINNYVKLQLIDKPERKKYYKTHIAQLIVITLLKQVLPLSEVKK
                     GLELQVSIRGFQIAFDTFCQELEYAFRMLFRDLDKKEHFTYTLEDIHSENIALKMITL
                     SLASKLLTQKIILVDGVSHASQKGENTNDEI"
     gene            13565..14425
                     /locus_tag="PLT73_00345"
     CDS             13565..14425
                     /locus_tag="PLT73_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM18861.1"
                     /translation="MKSNSIAVLADSCNDIPQELLGKYHIYILPLMINYKDASYRDRV
                     DITPEQVYERFQEEIPKTSLPLPETIADTFAKIKADGFNQVIVSAISSGLSGTCQAIR
                     LLADDIEDMQIVVIDTLNIAIASGFVALYAAEQIEAGLPFEEVVSKTQAAVKQSTILF
                     GVGTLEYLMKGGRIGKVSGILGSALNIKPIISCNEDGIYDTVAKVRGRKQSIQKLIDM
                     TREKLGQHKNYYLSICHGDAYEEMLVMKDQLKDLVAGAKIYAEGQISPVLGVHTGPGL
                     LGIGIMVLED"
BASE COUNT         3612 a         3718 c         3165 g         3947 t
ORIGIN      
        1 gacgctcaat ccgatgctgt cctcgtccaa cccgtccaaa gcaaagaata caggctcctt
       61 gcggtctgaa tatttgatcc cgaccacgtc tgtgtctttc attatttccc aaggcttcag
      121 tttatggaaa gcctttatgt tttcgtacaa tttttgataa tctctcattt atatctccta
      181 tctatttaca aaacataagc ctatgaacct ttataaaaat gaaattcgca gaagatgctg
      241 ctttttagtc agttatccct agcgctttcc accgcatatg ttgtttcagc ttattgtaac
      301 aaattccttt cctatgctat catatcttga agcgtgcatc aatttttctt ttttaaggag
      361 tccaaaaatg accttttctt tcaaggatat cgaacatagc aagtcagtag tcaaagaaac
      421 ctctctgtac actcactatc acaatgaaac ggcccttttt atgtatatcg aaaattacgt
      481 gattttccat cgcgtgccaa ccttcgctga attcatcgag gcggaagcct atttgaaatc
      541 agcgcatcaa aaagataacc aggatttcat aaaattcatt tggcccgaag attgcgaaat
      601 tccgcagccg atcatttcct atctgggttc gaacgacttc agcctcgatg tattggaact
      661 gtacgcggcc cctcccggaa cctttgagcc gacgcggatt gatgaaacac cttccgatgt
      721 caaagtggaa tgaacgccga caaccgcagc gcctatctgg ctttgtccgc acgggcagac
      781 aaggaagtca gcgaggaatt cgcaaaacaa aagcagccct acacccaagc taaattctac
      841 aacccagctt tccgtcccat cgtggccctg cgctccgatg aagcggtcgg ttccatcgac
      901 ctttatctga aagaggaaag tattgaaatc gataactttc acgttgctgt agattcccgg
      961 ggtaaaggga ttggaacggc tctgcagcaa ttcgtgatgg atcatgccga cgggaagacc
     1021 gtccttttag tggctgatgc agcggatacg gcgcgcgaga tgtacaatcg tcaaaattac
     1081 acatatattt ctttccgcta cgagctcctt aaagtcttca aataacggat caacgggctg
     1141 tctcatacgt agacggccta ttctgcaggc agcagccata actagagcga aagttgtccg
     1201 gtaatccgat tgaatcggac aaccagagtg ctaatagcaa caaaaagggc tgcatcagat
     1261 ccagagagtg ctacgctggt tctgatacag cccctattta cttttctcat tcgatttcgt
     1321 tgatttggta gatttccaca ggatagttcg attcgatttc ctcaatgatt ttatccacga
     1381 ttttttggca gaggacccgc gaactgttga caaccgccaa cccgatgacg gcctggttca
     1441 gcatatcttg tgccgatact tccgcaatgc tgacattgta ccgttgatgc atcttggcaa
     1501 ggatgctttt cacaacgcta cgcttatcct tcagagaatt ggaatcaaaa agccgcatag
     1561 acagttcaac agccaacaca ttcatcgtca tcgccccatt tatgtttgat atagaaatat
     1621 tatctcttca agacaaaaaa taaccgaaac ggccccttct gtcaagggat agctccggtt
     1681 atcggataaa acaaggacgg gattcatcgt atcctagtag ttgaactctg aattgcaggg
     1741 gattccgcct aagctgacat tacgtcacgg ccaaaaagcg gccgttctcg taatgtcctc
     1801 ttagtgctca tcccctaccc gcaattcatc gtatcctagt aattatctac atcttttctt
     1861 tcgcctttga agttgttttt cttttggtgg cgttcgatga ggactttggt cacatcggct
     1921 acgtcgacgc cttggtcgta caggagtacg ccgaggtgat atagcaggtc tgccgtttcg
     1981 ttggcgactt cttctttgtc gtggtttttc gcgccgatga cgacttcgaa ggcttcttcg
     2041 ccgaatttct tggcgatttt atctgtgcct ttgtccagca gatagtttgt ataggacttt
     2101 tcatcgtgtc cgacagcgcg ttggcggatg gtctcttcca ggtcatccag accaaaagca
     2161 accgggacat cgaaacaact ctccgtattg cggtggcaag tcggtccggc cgggtcgacc
     2221 atgatcagca acgcatcctg gtcgcagtcc aggtacatgt ccttgacgaa ctggtaatgc
     2281 tcactggact cgcccttttt ccagagtctg tccttgcttc gggaaaagaa ccagacaacc
     2341 tcgtcccgtt tcgtcaattc gaaggcctct tcattcatga agccgaccat caaaacattt
     2401 ttgttcgtaa agtgctgaag aacgacagtc aataaccctt tcgtaaagtc aggcactagt
     2461 gcatcgtatt tagacattgc gcatatccac tccattctcc gtgcagcatt ctttcacttc
     2521 ggcgatcgac acttcttcat catggaagat ggaagctgcc aatccggccg atacatccgt
     2581 ttccttgaat aagtcggcga aatgctgggc attgccgccg cctccggaag cgatgaccgg
     2641 gatcgagacc aaagcttgga ttttattcag taaacggtga tcgaagcctt gcttcatgcc
     2701 gtcataatcc atactggtga ccagcaattc gccggctcct agtgcttcca cttgctgcac
     2761 ccagtccaac gttttgatgt cggtccgttt tttgccgccg tgggtatagc aataccattc
     2821 ctctttgtcg gcttcccatt tcgcatcgac agcgatgcag atgcattggc tgccgaactt
     2881 gtcgctggct tctttgatca gttgcggatt cgctaaagcc gatgagttca ggctgacttt
     2941 atccgctccg gcgttcaaaa ggcgggaaat gtcgtccgtc gacttgatgc cgccgccgat
     3001 cgtcatcggg atgaacagct gttgcgccgt tttgcggatg acatcgatca tcaagttgtg
     3061 cccggtttcg gttgcggaga tgtccaaaaa gaccagttca tccgcaccca aagcattgta
     3121 gcgcttcgcc agttcgaccg gatcgccgat ttcgcgcaat cccttgaatt ggatcccttt
     3181 tacgacggtg ccgtccttca catccaggca gggaatgatc cgtttcttta tcatgccaag
     3241 ccgactatcg ctgccgccac acccaaagct tccaattttt tgatgtcttc gatgttgcgg
     3301 acgccgccgg atgcggtgat gggatggttc gagagctcga caagcttgcc cgtcaactca
     3361 aagttcggtc cggccatctt gccgtctttc gagatatccg tatagatgat gccgcccaag
     3421 gccatcggat tcgtcgcttc cacgacatca tagatggtct ggcggcccgt ttcggtccag
     3481 ccgttcacgg cgaccagttc gcctttagca tccagcccca ggtagatctt gccggggtat
     3541 ttcgccgtca tctcagccag ccacggcaga tcctgtagcc ctttcgtccc gacgatcaca
     3601 tagctggcgc ctttttcgaa atagtgctta atggtttctt cggaacggat gcccccgccg
     3661 atttcgacag gcaatgcact ctgttgcaaa agttgctcga tgaacggcgt ctcttccggt
     3721 ttttggttca aagcgcccat cagatccacg atatggatgc gcttcacttg ctcaaattgc
     3781 gcataaaaag caatggcttc ctgaggcgtg cgctgcattt cttctttcgt gccgtagtct
     3841 ccttcggtca gacggacact cttgttgtcg atcaagtcga tcgccggcca aatctctatc
     3901 atttatttcc atcccccttg caaagcttgg ttcaaaattt ccagaccgta atccccgctt
     3961 ttttccgggt ggaactggat gccgatgacg ttatccttct ggacgatcgc cacgatttcc
     4021 tggccatagt ccgccgttgc cacaacattt tcgtcggtca ctaccttgta ggaatggatg
     4081 aaatagacat ccttatccaa acctggatgg cttgaatgca gattgttcca gcccaaatgc
     4141 ggaaccggca agtcgctgac gatgtatttc acttcgcccg gcaaaaagcc caacccatcg
     4201 acatcccctt cttcgctgtg ctcaaacagg agttgcatcc cgagacagat gccgatgaac
     4261 ggctttgtct gctccaactc agtcagcagt tcccgcaaac cgattgcatc gatccgctcg
     4321 atggcatcct tgaaatgtcc cacacccggc aggacgatga catccgcctt gcgtaattct
     4381 gcttcttcat gcgtcaagat cgtttcatag cccagaaaac ggatggcgtt cgtcaggttc
     4441 gcgatgttcc ccaaaccata atcaacgatt gctatcattc gatcactcct tttgaggaag
     4501 gcaagcgctg cacgtcggaa tcggccatcg cgatgcgcag gctttgcgca aaagctttga
     4561 agatggcttc gatttcatgg tgcgtattgc cgccgcggat caagtcgata tgtgccgtgt
     4621 aacgggcatt gatgaccaat ccatggaaaa attcttcaac caattccaca tcaaagctgc
     4681 caactttttc ttttgaaaac gaggcattga agctcagata aggacggcca ctgatgtcga
     4741 tgacggaacg ggccaaggtt tcatccatcg gcacataggc actcccatag cgattgattg
     4801 cccctttatc ctgacccaat tcagccaata gctggcccaa tacgatcccg acatcctcgg
     4861 ttgtgtggtg atcgtccaca tctgtgtcgc cgtccacctg cacatccaaa acgaagtggc
     4921 catggaaagc gaacagatcc aacatatggt tcaagaagcc cacacctgtg ttgatgacgg
     4981 atggttctaa gcctcgtttc aaggtgatgg cgattttcgt ttctttggta atgcgctctt
     5041 ttgatacgct cacagctgtt ctctccattc tctgatgatt tcgcctaagg catccaactg
     5101 ttcatccgtg gcgatcgagt aacgcacagc cgtttcgata cacgggatgc ctgatttttc
     5161 ataagtccgc ggctgatagc cgtggtcgat gatccattgg cccaaagacg gcgccaattc
     5221 accgtaagtg aagacgaaat tcgtctcgct cggcagcacg gaaagaatat ccgctacacc
     5281 ttcattgaag attttcttca gtttggcgga aagccggcgc tgatcagcca tgaaggcccg
     5341 caaacgttcc ggatgctcca gtaagtaggc cgctatgctc aaagataaag tattgagcgg
     5401 atacggatgc gcgatggagt tcaacaactg catcgttttt tccgtcgaac tggcgatgcc
     5461 gatgcgcagg ccggccaagc cgtagatttt tgacaaggta cgcaaaagga tgacgtggtc
     5521 accgaccgga cgcgccggtg gattgtccac gaaatccatg taagcctcgt ccaaaatcag
     5581 atagccgccg gagtcagcca gcagatctgc cgttttttga atgaaagctg cagggtgcag
     5641 cccgcccaac gggttgttcg gttgcgaaac gatgaaatag gaaggctttt ccttctcgat
     5701 gacagcgtac acttggtcat agtcaaagga ataatccgct cggcacggca ctttgatgat
     5761 ttcgcgctgg aattgcgcgg catactcttc gtacatcacg aaatccggct ccagcatcag
     5821 gatcggcccg tcccccaaaa ggattgtgca cttctggatc cactcatccg atccgttggc
     5881 aaaagcgatc agggctggat cttctccgtg gaaagcggcg tacgcttccg tcaggcggtc
     5941 gatggcatcg tccgtatact cctggaagcg tgtcgataca gctatttcgg caatttcttc
     6001 atttgtcagc gcccggatcg gactttcatt tttgttgatg cggatcattt ctcgtccccc
     6061 attctgacag ccagggaatc gtggtgggct tgcagtgact cttcagtcgc aatcagcatc
     6121 ccggccgggg ccattttttg gaacgtttct ttcttcaggt tcaaaacaga gttcgggcgc
     6181 aggaagtcat ttacggacaa gccgttcgca aagcgggcat tccctccggt aggcagcacg
     6241 tggcttggtc cggcaacata atccccgatc gcttcaggcg aatattcgcc gatgaagatc
     6301 gctccggctg tttcgatggc atcaatgtaa tcttctgcgt cttttgtctg gatcgagacg
     6361 tgctcgcctg caatcagatt cacgacttcg atgttctctt ctttaccgct cgtcaaaatc
     6421 gggaagtgat tgtgcttcaa actctcctcg atgacctcga tgcggctttg tttcgctttt
     6481 tggacaagca gttcggcttc aatggcggcc aatttttctt cgttgtcgca gacaaggaaa
     6541 gtgcgggcca attcatcgtg ctcggcttgc gccagcaggt cgatggcaac ccattcgttg
     6601 ttcgccgttt catcaacgac tacaacgatt tcggatggtc cggcgatcga atcgatgccg
     6661 acatctccgt agaccaattt tttggccaag gcgacatatt ggttcccggg tccgacgatt
     6721 ttgtcgacgc gcggaatggt ttccgtaccg taagccaagg cagccacggc ttgggcgccg
     6781 cccacttgat aaacgtgggt cacgccgcag atatagcaag cggccaaagt tgcttggttg
     6841 atggcggtct tttgcggtgg ggtcacgacg atcgtttcct ttacgccggc tacattggcc
     6901 aaaacggcag tcatcagaac tgtggaagga tagctcgctt taccgcccgg cacatagatg
     6961 ccgactttgt tcagcggatg gatcttctgg tacagttccc ctgccggaga ctgttgccat
     7021 ttgatgcttt cctggtagac cgtgatgttg tcgcgggcct cctccaaagc agcgcgcaag
     7081 accgcatcca agctgtcata agcttccttg atgacgctct gcggcacttc caattccgtg
     7141 atgtccgccc catcgaaacg ttttgcgtat tcgaacagcg cagcgtcgcc tttttcctga
     7201 accgtctgga tgatttccat gacagcctgc gtcttcgaaa agtcgattgt attttttgtt
     7261 ttgtatactt ccttgaacgt tttagttgtg tacattattc gtcaccttca attgattgat
     7321 tatagtctgg atttcatcat atttggaaaa ataagcatgc ttattcgaaa tcaggcgtgc
     7381 gttgatgtcg tcaagccgga cctgctcgct caacnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagcttgat gatcttcacg ggttgcttga
     7501 tggaatcaaa atattgtctg gtgaagttca cgtactttgt tgcaaccact ggataaacgt
     7561 ccttcttctc tttgctggcg atggcgaaat gacaatagcc gaatggcaaa tccatcagat
     7621 tgttgatgtt gcagttctgc tcgatcagaa tatcgcttcc ggtgatgccc agatccgcga
     7681 tcccttcctc cacatataca gggacatctt cccctttaac caaaatgaag cgggtatcct
     7741 ttgtctgaat gaccaattcc cgagaaacgg acttcaatgc tgtcgaccat tccgtcaggt
     7801 tgatttcatc caaatattgg atgaaatcct taagctgtct gccttttgat agcgcgatag
     7861 taatcatttt ttccctccgt taattgttga tggccattcc gaatgctttg gaagagcttg
     7921 tgtattgacc gcccgacgct acaggttccg tttggttttc tccgtacagt tgaataaaga
     7981 ttcccttgta ataggattgg ttcggcaacg ccagcatatc cgcgtagacg taatctacgt
     8041 tcttgctctt cagcgtcgct tcccattccg ccaattcatt caaatgtccc gtcagttcag
     8101 gatagtgttc cttcagatag ccggcctgtt cggtaggcgc ttggtccatc agaccgatca
     8161 gcggatggtc cgcacccaaa cgatgcttca aggcatcctg gtttctttcg cagatgaact
     8221 tgcggacaga agcatcttcc aactcccgcg ggctcagaat ttttttcagt aatttattat
     8281 ggctgactac cgcaaccgat aaggaaagtt ccaattcctt ctccatgaag gtgatcatat
     8341 cgcccagaac ctcgatttgc ttcggaatcg agtcggtaaa tgtttccaca cccaattggt
     8401 ggcgctcttt gagcagcgaa tagaccggac cggagtaggc tattttatcg gcgcgtaaat
     8461 ggtattgctt gcggtatctc acgatggcat tcgtccagtc actgcgcaag gcgaacagtt
     8521 tatcgccgct ttgccatttg tgccggttca gcatcaggtg caagtcatca tgagacagtt
     8581 gtgtccactc aaatttctct acgactccca ggtcgatcag atcgtaccct aagtggtgga
     8641 aatgatggag aaaccccatc tctctctgtt tcttcgctaa caacgattca ttggtcatca
     8701 tttcgtccaa actgtctcac tcccatttct ttttgatagg tcgtctgtta tccttagaat
     8761 atcgttagaa taccattaga tgttgatgtt atcataccat agaacgctgc tataatgtat
     8821 cctaaaatga tgatacttta tgatgaaagg aagctgtata aaatatgctc agcgacagac
     8881 acgtccatac cccctattgc ctgcacggtt catccgatgc aatggaaaat tatgtgaagg
     8941 ttgccatcga ggccggcctc gagtccctca cattcacgga acacgctccc ttaccgatgg
     9001 cagatccgct tccggacaaa gacagttcga tgcgacctga ggatgtcgat gcctacttgg
     9061 ccgaagtccg tacacttgcc aagaagtatc aaggcatcat cgaaatccac gccggttttg
     9121 agttggacta cctcgaagga aaggaaccgg aaacacgtgc ctttctggaa aaatatcccg
     9181 agacagtccc tcactcgatc ctgagcgtgc atttcgtgca gctcgctccg gaagagtact
     9241 tctgtatcga cttggactgc gaatcgttta cgcaaaaagg cgcagaaata ggctacgaaa
     9301 cactttacac actttacgaa gcagcggtca aaaaagcctt ggcatccccg tacggagaat
     9361 tgaccccgaa aaaaatcggc cacatcaact tgatacacaa gttccaaaag gcctacagcg
     9421 tcagcgaccc catcgactgg aagttgctgt tggataaggt gaaggagaac ggatacacgc
     9481 ttgattacaa ctttgccgga atcgacaagc cggactacgg aaaaacgtac ccggacaagg
     9541 aaatgatggc ttacgcgata aaaatcggcc tctcctatga gatgggttcc gatgcccatg
     9601 cttcccatga agtcgcccgc tatttcggta cagcggtagt agaaacggaa ttatagaaaa
     9661 aaataagcag ccaagcggtt ggattcgctt ggttgcttat ttgcttactg gtaaatcgct
     9721 ttgatcggat ccattcttga agcgctcaga gctggaataa tcccaaataa aagtcctgtt
     9781 ccgattgata cagaagtgga tagaatcatg atggtcggcg ttaagaatac cggcatttgc
     9841 aaaagcatac caactaaact cgcaatccca tatcctnnnn nnnnnncaaa ccgataatcc
     9901 caccaagaaa cgtaataaag gccgcctcca ataagaattg caacagaatc attctcggtt
     9961 tagccccgat tgcacgacga ataccaattt cgcgtctccg ttctgttaca gatacataca
    10021 tgatattcat aacaccgatt ccaccgacaa atagggatat ggctgtaatg gccagcaaga
    10081 acatcatgat tccattgatg taactgttga cctcttccat catctgttca ttggagcgat
    10141 cttgttcaaa gaccccctca tcttctggat aagcttccac cagattttcc aagacggtca
    10201 ttgtgaccct ttcattatcc attccttctg ccactttcac tttcaatgat ttaatcggct
    10261 tagagcgcgt caaagtattg taagctgctt gaggaatgac actcgtataa gcgtcttccc
    10321 atgtataacc cgtatttagg agattactgt cctcccagtt gtagggctcc ttgattccaa
    10381 taatctcata catgaatcca ttgatactga cagcttgacc aatcagcgat tcgggatctt
    10441 cgaccacgat accgtaatcc aatatatcgt gagataagat aatcatgtct cggtttacat
    10501 ctgaaggctg aaaatcgcgt ccatacaaaa tatggttttc gcttttattc ggcaccatca
    10561 tcgtatatgc ttgcgctcca aaataatcca aattgacgtc aatcgagtcc atcgccatac
    10621 catttgaacc ataatcgggt acaaccgact gaacgccatc caaccgggaa atccgtcgca
    10681 tatccgcttt tgaaaaagaa aaatcatctt ccacataacc cattccactg ttatcactca
    10741 ttgcgattgt gtgtttcaca gtcacaacat tcgcttccgt cgtattcgcc aattccatac
    10801 tttgttgctt cacgccttca cccagtgcag caatggtcac gaccgaagca attccgatga
    10861 taatccctac catggttaaa aaaacgcgaa gcttgtgtgc ccggagactc aacattgtac
    10921 tgagcaagat attacgaatc atcttacggc accttcttcc acgaaactcc catcatgcat
    10981 ccgaataatt cgcgttgcat actgcaataa atcttcgtca tgcgttacca taatgattgt
    11041 tttcccttgc ttatttaatt ctcgcagcag gctcatcacc ccaattccgg tgtcggaatc
    11101 gagcgctccg gttggctcgt ccgccataat gagaagcggt tcattgatga gggctcttgc
    11161 gattgcgacg cgttgttgtt gtccaccgga aagttctttg gttttggctt ttatcttttc
    11221 aggaataccg actaaactta aatacttctc agcagcttcc aaccggtctt tcttgttcac
    11281 ttcgccagca taaatcaatg ggagctcgac attttgggcg acattcaaag aatcaatcaa
    11341 gaaaaactgt tggaaaatga aaccgaaatg tttgttgcgt atactggatt ttttattttc
    11401 attcaacagg gatacgtctt cacccaaaaa tgaataggta cccgttgtta agcggtccaa
    11461 aaagccaatc atattcatca aagttgtttt tccacttcca gatttaccga caatcatcaa
    11521 gaagtcgcct tcatgaatag tcaaattaat ctctttcaat accaccgttt ctaactcgcc
    11581 tttcccaaac tttttggtca catctttcat ctgaatcagt gacctgcgtt ttgttgcctc
    11641 catcttattc ctcctccagg tccacttcca tcgaagtagg atctaaaaga tcaccttctt
    11701 gaacatccgc ttcagaagaa acaataattt cgtcttgtgc ttctaatccg cttgttacta
    11761 cgataccttc tgcggcctct tcagtcgttt ggataacgcg gcgtgtgaca gtaccgaaat
    11821 catttaccaa gacatatgtc acatcaccgt ctgtacggat ggctactttg ggaacaacaa
    11881 cttgattttc ttctaggttg atggttgctt gcacatgata gccattcacg atacgttcta
    11941 atgaatcaaa actcaatttc acagggtaac tcgacataga ggatccttga gtgtatggat
    12001 ccgacactgt gttggcaggt gttgtatcga caaaagacac tttccctacc gtggtgaaat
    12061 tactggaaac gaccgtaata tcagctactt gaccaatcgt tactttttcc aaatcttttt
    12121 cattcactgt tcccgcaaca taaaaatttt ccgaaattaa tgtcagtaca ggcacatctg
    12181 ctttcttttc ttccggaata accactttac cggtgaatgg tgcggtcact tctgtataga
    12241 cggactcttc caacgataag atattgttgt atatttcctt gatttgcgca tccaagtcat
    12301 ataaattgaa gtccattatc aattcatcca atgtttgagc ccgttcttct tcctgcattt
    12361 catagaagcg atcaatggca agttgctgtt tgtaggctgt attcgcccgg ttcgtctcaa
    12421 gccgcgcagc ttcgttatac aattgcgtaa cttctttctg cactgtttca tttgtatagc
    12481 tgtacagtaa ggtccctgcc tccaccactt gcccattttc tacttggaga tcccctcttg
    12541 tacctaagtt ttcttcttga tagaactcct gagattgttc aggcgtaacg acaccgttca
    12601 caaaaatttg ttctatcgcg ggcactgtat aatattcaat gccatacgga tcctcttcca
    12661 ctatcggttc atcctctgct ggtttcccta aataattcgg caaaaagatt acccctaaca
    12721 ctgctacaac tgagataatg cccatccaaa gccacttttt ctttttcaca ctccaacctc
    12781 ctatatagtt acgtttgagt gtattatacc cttaatacac caaaatgcaa tccttttcat
    12841 aaaaaggact gctcctttaa gcaatccatt taaattatca ctgaagtttc aagattgaat
    12901 tttccgccaa atactggtac atttatctag taacgaaaaa cattacatct agtgtcgaaa
    12961 actagattga atttcccagt ccagaaagca ggttgtcccg tgaaacttaa accagaattg
    13021 gaagactatc agcaacagct gctcaacttg acattcatcc gatttgacga attacctgat
    13081 tttggcctct acagcgatca agtgatcgcc atcatcgaaa agcaattaag tttcctgaat
    13141 gccacccagg aagaacgcat catcacgccg gctatgatca ataattacgt aaaactgcag
    13201 ctgatcgata aacccgagag aaagaaatat tacaaaacgc acatcgctca actgatcgtc
    13261 atcaccttgc tcaagcaggt gttgcctttg tcggaggtga aaaaaggact ggagctgcaa
    13321 gtctccatcc gcggattcca gatcgccttc gacactttct gccaggaatt ggaatacgcc
    13381 ttccggatgc ttttccgcga ccttgataaa aaagagcatt tcacctatac cttggaggac
    13441 atccacagcg aaaacatcgc tttgaagatg atcacgttgt cgcttgcttc gaaattgttg
    13501 acacaaaaaa tcatattggt cgatggcgtc agtcacgcat cccaaaaagg agagaataca
    13561 aatgatgaaa tctaattcca ttgccgtctt ggccgattcc tgcaacgata ttccacagga
    13621 gttgctcggc aaataccaca tctacatctt accgttgatg atcaattata aagacgctag
    13681 ctatcgcgat cgggtcgaca tcacaccgga gcaagtctac gaaagattcc aggaagaaat
    13741 tccaaagaca agcttgcctt tgccggaaac gattgccgac actttcgcca aaatcaaagc
    13801 ggacggcttc aatcaagtaa tcgtatccgc catttccagc ggtttgagcg gcacctgcca
    13861 agccatcaga ttattggcgg acgatatcga agacatgcag attgtcgtca tcgacacgct
    13921 gaatatcgcc atcgcatcag gatttgtggc actttacgct gccgaacaga tcgaagcggg
    13981 acttccgttc gaggaagtcg tttctaaaac acaagcagcc gtgaaacagt ccactatcct
    14041 cttcggcgtc ggcaccttgg agtatctgat gaagggcggc cgcatcggta aagtttctgg
    14101 aatcctggga agcgccttga acatcaaacc gatcatctcc tgcaacgaag acggcatcta
    14161 cgacacggtc gcaaaagtgc gtggccgcaa acaaagcatc cagaaactga tcgatatgac
    14221 ccgcgaaaaa ctgggtcagc acaaaaatta ctacctttcg atctgccatg gggatgccta
    14281 cgaagagatg ctcgttatga aagaccagct gaaggacttg gttgctgggg ccaaaatata
    14341 cgctgaaggt caaatctcgc cagtattggg tgtccacacc gggcccggat tgttgggaat
    14401 cggcatcatg gtgcttgaag attaaaacag agtgtgatga ttcgcgggag gggacgagca
    14461 ctaagaggac taacacaaaa cggacgtttt ttgtccgtgt gtggtagtc
//
LOCUS       DAOMUJ010000005        14493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_892_length_14493_cov_3.091287, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000005 DAOMUJ010000000
VERSION     DAOMUJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14493
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_14493_cov_3.091287"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1099
                     /locus_tag="PLT73_00350"
     CDS             362..1099
                     /locus_tag="PLT73_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRM18862.1"
                     /translation="MIIIVYISIFYFGAIFGSFFTVLGTRIPIKENFTTTRSHCVHCQ
                     HTLQTTDLFPIVSYVCHRGKCAYCATKIDPMYFASEIISGFVTCLLYYFYATDLLQLL
                     ALAFMFSLLLVISITDYLYLLIPDRFQLLLFFGVVFHQMTLPTTDWPAAFISFFVIFS
                     LLFFTGLALPDGIGGGDVKLLSILSLAFGLEPTLFIILLASLFAGFCFLHTYLTNGSS
                     LQRRLPFGPFLAASAVVVHFAQMVLVL"
     gene            complement(1384..5028)
                     /gene="rpoC"
                     /locus_tag="PLT73_00355"
     CDS             complement(1384..5028)
                     /gene="rpoC"
                     /locus_tag="PLT73_00355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM18863.1"
                     /translation="MIDVNKFESMQISLASPDKIRSWSYGEVKKPETINYRTLKPEKE
                     GLFCERIFGPQKDWKCSCGKYNGIRYKGIVCDRCGVEVTRAKVRRERMGHIELAAPVT
                     HVWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGDTVLESKQLLTEREYRDKRA
                     QFGNSFHAAMGAEAIKQLLDRVDVDTECEQLKEDLKSATGQKRTRAIRRLDILDAFRS
                     SGNKPSWMIMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PNIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFLMKELVARDIASNIKNAKRKIDR
                     MDDDVWDVLEDVIREHPVLLNRAPTLHRLGIQAFEPVLVEGKAIRLHPLVCEAYNADF
                     DGDQMAVHVPLGEEAQAEARLLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLEEIN
                     KVGEGMIFSNAEEAGLAYEGGFVHLHTRVAISTNSLPKKPWTEWQRGRLLVTTVGKIF
                     FNEIMPDEFPYLNEPTQFNLDVATPDKYFVEAGEDVQAFIKKQEIVGPFKKKYLGNII
                     AAVFKKFQVTETSKMLDKMKDLGYKISTRAGITVGIADILVLESKAEILERAHKKVDN
                     ITKQFRRGLITDDERYNSVITTWNMAKDEIQIELMKTLGQENPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGKIMELPITSNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLIVRSIQEGNEVIEALEERLIGRYAQKTVVHPE
                     NGKVLAVHNQLITEDMAKEIIDAGIEEVTIRSVFTCNTRHGVCKRCYGRNLATGSNVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRIQEIFEARNPKGQAVIT
                     EVTGEVVSIDESASDRSKEVTVKGITDTRTYTIPYTARTKVAEGDIVHRGDPLTEGSI
                     EPKSLLRVRDVLSVETYLLREVQKVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGSTD
                     ILPGTLMDISDFEDRNRAVINAGEVPATARPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDSLLGLKENVIIGKIIPAGTGMARYRKMEPKAVEAESESVYSITDTAE
                     MGSDLSDYRG"
     gene            complement(5148..8675)
                     /gene="rpoB"
                     /locus_tag="PLT73_00360"
     CDS             complement(5148..8675)
                     /gene="rpoB"
                     /locus_tag="PLT73_00360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM18864.1"
                     /translation="MLGHNEKFGKQRTRRSYSRISEVLELPNLIEIQTDSYKWFLETG
                     IKEMFKDITPIEDHTGNLALEFVDYEFHAPKYTVSEARAHDANYSAPIYVKLRLINKE
                     TGEIKDQEVFFGDFPLMTDMGTFIINGAERVIVSQLVRSPGVYFHNKMDKNGKESFGN
                     TLIPNRGAWLEYETDAKDISYVRIDRTRKIPLTVLIRALGFGSDDQILEIFGDNESLR
                     LTMEKDVHKNASDSRTEEALKDVYERLRPGEPKTADSSRNLLNARFFDPRRYDMAPVG
                     RYKVNKKLNVKTRLYNQTLAETLVDPETGEIIAEKGTELDRGVMERLAPYLDNGLNQV
                     TLYPTDDGVVTDPVVMQVVKVYSKKDPEKVINIIGNSEIADDIKHLTTADIFASMNYF
                     FNLSEGLGTTDDIDHLGNRRIRSVGELLQNQFRIGLSRMERVVRERMSIQDVSTVTPQ
                     QLINIRPVVAAIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKINKYGFIETPYRRVDWNTHKVTNIID
                     YLTADEEDNFTVAQANSVLLEDGSFANNIVMARYIHDNLEVPIERVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPFVGTGMEYKAAHDSGAALLCQN
                     DGVVEYVDAKEIRVRRDNGALDKYEIIKFQRSNSGTCYNQRPIVALGDRVDVGETLAD
                     GPSMEKGEMALGQNVLVAFMTWEGYNYEDAVIMNERLVRDDVYTSIHIEEYESEARDT
                     KLGPEEITRELPNVGEDALKDLDERGIIRIGAEVKDGDILVGKVTPKGMTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNLLVRVYIVQKRK
                     INEGDKMAGRHGNKGVVSRIMPEEDMPYMPDGTPVDIMLNPLGVPSRMNIGQVLELHL
                     GMAARSLGIYVASPVFDGADEDDVWGTVAEAGMANDAKTILYDGRTGQPFDNRVSVGV
                     MYYLKLSHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGETIPRPGVPESFRVLVKELQALGLDMKVLDEAEE
                     EIELRDMDEEDDVVNMERLNKQSEAMKRD"
     gene            complement(8985..9593)
                     /locus_tag="PLT73_00365"
     CDS             complement(8985..9593)
                     /locus_tag="PLT73_00365"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM18865.1"
                     /translation="MSNHYYTKNPETESKEASWTFPLRGREFRFISDSGVFSKKTVDF
                     GSRLLIETFRLNEEVAGDILDVGCGYGPMGLALAYAYPARLVEMVDVNERAMSLARRN
                     AEANNIRNVKVYESNTYDQVPEERQFAAIVSNPPIRAGKQVVHRILSEAHVHLLPGGT
                     LTVVIQKKQGAPSAQQKMLDIFGNVAVIARDKGYWIIQSVKA"
     gene            complement(9727..10092)
                     /gene="rplL"
                     /locus_tag="PLT73_00370"
     CDS             complement(9727..10092)
                     /gene="rplL"
                     /locus_tag="PLT73_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRM18866.1"
                     /translation="MSLNIEAIIADIKVATVLELNDLVKAIEEEFGVTAAAPVAAAGA
                     VAAAEEEQTEFNVELTSAGDSKIKVIKVVREATGLGLKEAKALVDGAPGVIKEAVSKE
                     DAEALKAQLEEVGASVTVK"
     gene            complement(10178..10678)
                     /gene="rplJ"
                     /locus_tag="PLT73_00375"
     CDS             complement(10178..10678)
                     /gene="rplJ"
                     /locus_tag="PLT73_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRM18867.1"
                     /translation="MSEAAIAQKQLLVEELTTKIQESASVVVVDYLGLTVEQVTELRK
                     QLREAGVEMKVIKNSLMVRAAVAAGLEGMESIFKGPTAVAFSSEDVVAPAKIMAEFAK
                     TADKLEIKGGIVEGNVSSKEEIEALAKLPNREGMLSMLLSVLQAPVRNFALAIKAVSE
                     KDGEAA"
     misc_feature    complement(10716..10842)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(10883..11572)
                     /gene="rplA"
                     /locus_tag="PLT73_00380"
     CDS             complement(10883..11572)
                     /gene="rplA"
                     /locus_tag="PLT73_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRM18868.1"
                     /translation="MAKKSKKFQDAAKKVDSTKAYSVEEAVALAKEVDFAKFDATVEV
                     AYRLGIDTRKNDQQIRGAVVLPNGTGKTQRVLVFAKGEKAKEAEAAGADYVGDADMVQ
                     KIQGGWFEFDVVVATPDMMGEVGRLGRVLGPKGLMPNPKTGTVTMDVTKAIDEIKAGK
                     VTYRADKAGNIHVPIGKVSFDDAKLMENFRTIHDVILKAKPATAKGQYVKNVSVTTTF
                     GPGVKVDASSL"
     gene            complement(11677..12102)
                     /gene="rplK"
                     /locus_tag="PLT73_00385"
     CDS             complement(11677..12102)
                     /gene="rplK"
                     /locus_tag="PLT73_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRM18869.1"
                     /translation="MAKKVVKLVKLQIPAGKASPAPPVGPALGQAQVNIMGFCKEFNA
                     RTADQAGMIIPVVISVYEDRSFTFITKTPPAPVLLKKAAGIESGSGEPNKKKVGTVTR
                     DQVKEIAETKMVDLNAADVEAAMRMVEGTARSMGITVQD"
     gene            complement(12230..12778)
                     /gene="nusG"
                     /locus_tag="PLT73_00390"
     CDS             complement(12230..12778)
                     /gene="nusG"
                     /locus_tag="PLT73_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRM18870.1"
                     /translation="METIENEKQWYVLHTYSGYENKVKLNIESRAQSMNMEDHIFRVV
                     IPEEEQVEVKDGKEKATTQKTFPGYVLVEMIMSDEAWFVVRNTPGVTGFVGSHGAGSK
                     PSALLNEEIEVILKRMGMSTRTHDVNFEKGEPVTITDGAFNGLSGFVEEVDSEKGKLK
                     VMIEMFGRETIAELDYNQVDKG"
     gene            complement(12920..13090)
                     /gene="secE"
                     /locus_tag="PLT73_00395"
     CDS             complement(12920..13090)
                     /gene="secE"
                     /locus_tag="PLT73_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRM18871.1"
                     /translation="MKFLKSVADEMKQVTWPTGKELAKYTNTVVVTTILFGIFFAVVD
                     FGITEAMSLILN"
     gene            complement(13126..13278)
                     /gene="rpmG"
                     /locus_tag="PLT73_00400"
     CDS             complement(13126..13278)
                     /gene="rpmG"
                     /locus_tag="PLT73_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012211413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM18872.1"
                     /translation="MAIRKAALACTVCGSRNYTITPTEKGRTERLEVKKFCRYCGKHT
                     LHKETK"
     gene            complement(13470..>14493)
                     /locus_tag="PLT73_00405"
     CDS             complement(13470..>14493)
                     /locus_tag="PLT73_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM18873.1"
                     /translation="GNVKTNWIWIVFIGIAYFVIYYFLFSFLIKKFNYKTPGREDDST
                     EVKLYTRADMNAKAQEGTSEGASVVKSETDEMSASIIYGLGGAANIVDVDSCATRLRT
                     TVANGDLVDAELLKGTGASGVIHKGNGVQVVYGPKVSNIKSNLEEFIESGSAAKLPSK
                     EDYYGLKAVEKTEEKVVETTAAPAVEEKATVATTLRSPIAGTAIPLSQVDDAAFASEA
                     LGKGVAIEPAVGEVVSPINGKVVMVFDTKHAIGLESDEGVEILIHIGLDTVNLKGEGF
                     TTHVKAGDLVEIGDKLVDFDIDFIKASGYKTTTPMVITNTFNYKNIEVVAEGTVDLGD
                     AIVTVQ"
BASE COUNT         3566 a         3586 c         2970 g         4371 t
ORIGIN      
        1 ctttgttcag ttttcaaagg tcaatcgcgc atcacgtatt acgtgattgc ttatttataa
       61 taccacttaa ccagcccggt gtcaacgcct tttcgaaata ttttttcgag atacttcaga
      121 cgttttttga actgcagtag caactcttat atattatcaa atccaggacc atctgtcaac
      181 aaatattttt catcaggaca aaggattaat ttgacaactt aaatagtcta ccacctgacg
      241 aacaccctgt caatactaaa tggaatgttt ttcaaaaaaa tttccccatc ctacgaacaa
      301 aaagattcaa cagactagtt gcggatacta taatcagttt gttcgtaaag gaggaattgt
      361 tttgatcatc atcgtttaca tatccatctt ttatttcgga gccattttcg gatccttctt
      421 cactgtactc ggaacgcgta tcccgatcaa agaaaacttc accaccacac gctcccactg
      481 cgtccattgc cagcatacac tgcagacaac agatcttttt ccgattgttt cttatgtatg
      541 tcaccgaggc aaatgtgctt actgtgcaac aaaaatagat ccgatgtact tcgcttcgga
      601 aatcatttcc ggttttgtga catgccttct ctattatttc tacgcaacag acttgttgca
      661 gttactcgct ctcgccttta tgttctcgtt gctgctggtc atatcgatta ccgattatct
      721 ctatctgttg attcctgatc gctttcagct cttgttattt ttcggtgtcg tatttcatca
      781 aatgactctt cctactacgg attggccagc agccttcatt agtttttttg tgatcttttc
      841 attgctgttc ttcaccggac tcgctcttcc tgatggaatc gggggcggcg acgtcaaact
      901 gctttcgatc ttgagcctcg ctttcgggct ggaaccaacc ttgttcatca tcctgttggc
      961 cagcctcttc gcaggctttt gttttttgca cacctacctg acaaacggta gctccttgca
     1021 gcgcagatta ccgttcgggc cctttctcgc cgcgtctgcc gttgtggtgc actttgcgca
     1081 aatggttttg gtgctttgac gcctctctaa ttctgttcga ggattttatt tatgcacaga
     1141 aataacccgt caaaaccatt ttgacgggga aaatcagttc aaatcacagt cagaaaaacc
     1201 gccgaaacca ccttggcggg gaaaaatatc cgcggacagc atgatattcc ccgccaaacc
     1261 cattttcgca gggaatcacg ccaacaaacc cattttcaaa tcccaatcct aggaaaaaaa
     1321 acatacaaac gacaaaaaaa cgccccggaa aagcaaattg cttttccgga acgtttgttg
     1381 tttttatcct ctgtaatcag ataggtcaga acccatttcg gctgtatcag tgatgctata
     1441 gacgctctca ctttcggctt cgacggcttt tggctccatc ttacggtagc gggccatacc
     1501 tgtaccagca gggatgatct taccgatgat aacattctct ttaagaccca acaaggagtc
     1561 tttcttgccg cggattgcag catccgtcag gacacgagtc gtttcttgga aggaagcagc
     1621 agacaagaag ctgtttgttt ccaatgacgc ttttgtgatt cccaaaagaa ctggacgggc
     1681 tgttgctgga acttccccag cgttgatgac agcacggttg cggtcttcga aatctgagat
     1741 atccatcaat gtacctggca ggatatctgt tgaacccgga tccataacgc gaactttacg
     1801 caacatttga cgaaccataa cttcgatatg cttgtcgccg atttctaccc cttgcatacg
     1861 gtatactttc tgaacttcgc gcagcaagta cgtctcaaca gacaatacat cgcggacgcg
     1921 caacaagctc ttaggctcga tggacccttc agtcaacgga tccccacggt gaacgatgtc
     1981 gccttcagcc actttcgtac gtgcagtgta aggaatggta tacgttcttg tatccgtgat
     2041 ccctttgact gttacttctt tagaacgatc ggacgcactc tcatcaatgg agacaacttc
     2101 cccggtaact tctgtgatga cagcttgccc tttcggatta cgcgcttcaa agatttcttg
     2161 gatacgcggc aaaccttgtg tgatatcgtc tccggcaacc ccacccgtat ggaatgtacg
     2221 catggtcaat tgagtacccg gttcaccgat ggattgggca gcgatcgtac cgactgcttc
     2281 accgacctca acatttgaac cagtagccaa gttacggccg tagcaacgtt tgcagacgcc
     2341 gtgtcttgtg ttacatgtga acacggaacg gatcgtcact tcttcgatac cagcatcaat
     2401 gatttccttg gccatatctt ctgtgatcaa ttggttatga actgcaagaa ctttgccgtt
     2461 ttcaggatga accactgttt tttgagcata acggccaatc agacgctctt ccaaggcctc
     2521 aatgacttca ttgccttctt ggatcgaacg gacgatcaga ccacgatcgg tgccgcaatc
     2581 gtcttcacgg ataataacat cttgcgcaac gtcaaccaaa cgacgagtca ggtaacctga
     2641 atcggctgtc ttcaaggctg tatcggtcat ccctttacga gctccatggg tagagataaa
     2701 catttccaaa acgcttagtc cttcacggaa gttcgaagtg atcggcaatt ccatgatttt
     2761 accgtttgga gcggccatca gaccacgcat accggcaagt tgcgtaaagt tggagatgtt
     2821 accacgggct ccggaatcac tcatcatgaa gatcgggttt tcttgtccca gtgttttcat
     2881 cagttcgatc tggatttcat ctttcgccat gttccaagtt gtgatgacgc tgttgtatct
     2941 ttcatcatcg gtgatcagac cacgacggaa ttgtttcgtg atgttatcga cttttttgtg
     3001 cgctctttcc agaatttctg ctttggattc caataccaag atatcggcga tccctacagt
     3061 gatacctgca cgagtcgaga ttttgtaacc caggtccttc attttgtcca acatcttcga
     3121 agtttccgtt acttggaact tcttgaagac ggcagcaatg atatttccaa ggtatttctt
     3181 cttgaacggt ccgacaattt cttgtttctt gatgaacgcc tgaacgtctt cgccggcttc
     3241 aacaaagtat ttatccggag tcgctacatc caagttgaat tgcgttggtt cgttcaaata
     3301 cgggaattca tccggcatga tttcgttaaa gaagatctta ccgactgtcg tcaccaacag
     3361 acggcctctt tgccattctg tccatggttt cttaggcaac gaattcgtgc tgatcgctac
     3421 tctggtatgc agatggacaa atccaccttc ataagccaga cccgcttctt cagcgttgga
     3481 gaagatcatt ccttcgccga ctttattgat ttcttccagc gtcaggtaat agttacccaa
     3541 gaccatatcc tgggatgggg ttacgactgg tttaccatct tttggattca agatgttctg
     3601 tgcagcaagc atcaatagac gcgcttctgc ttgtgcttct tcgcccagcg gtacgtggac
     3661 ggccatttgg tcaccatcga aatcggcgtt ataggcttca cataccaacg gatgcagacg
     3721 gattgcttta ccttcgacaa gtaccggttc aaacgcttgg atacccaaac ggtgaagcgt
     3781 cggtgcgcgg ttcagcaata cgggatgttc tctgatgaca tcttccaata catcccagac
     3841 atcatcgtcc atacggtcga ttttgcgttt tgcatttttg atgttgcttg cgatatcacg
     3901 ggcaaccaat tctttcatca agaacggttt gaacaactcg atcgccattt ctttcggcaa
     3961 gccgcattgg tacattttca ggaaaggtcc aactacgatt acggaacgtc cggagtagtc
     4021 aacacgttta cccagcaagt tttgacggaa acgaccttgt ttccctttca acatgtgcga
     4081 aagtgatttc aatggacggt tacctggtcc tgttacagga cggccgcgac gaccgttatc
     4141 gatcaaagca tcgacagctt cctgcagcat gcgtttttca ttctgaacga tgatgttcgg
     4201 tgcgttcagg tccaacaagc gtttcagacg gttgttacgg ttgatgacac gacggtaaag
     4261 gtcgttcaag tcacttgttg cgaaacggcc gccttccaat tgaaccatcg gacgcaaatc
     4321 tggagggata accgggatta catccatgat catccagcta ggtttgttcc ctgaagaacg
     4381 gaaagcatcc aggatatcca aacgacggat tgcacgcgta cgtttttgtc cagtcgcgga
     4441 tttcaagtct tcttttaatt gttcacattc agtatcgaca tcgacgcgat caagcaattg
     4501 cttgattgct tccgcaccca tggccgcatg gaaactattt ccgaattgcg cccgtttgtc
     4561 acggtattct ctttccgtca gcaactgttt cgattccaag acagtatcac ctggttcgat
     4621 gacaacgtag gaagcgaagt aaatgatttc ttccaatgca cgcgggctca tatccaatac
     4681 aagtcccata cggctaggaa tccctttgaa gtaccataca tgggttacag gagcagccaa
     4741 ttcgatatgg cccattcttt cacgacggac cttagcgcgg gttacttcaa cgccacagcg
     4801 atcgcaaacg atgcctttgt aacggatacc attgtattta ccacatgaac atttccagtc
     4861 cttttgcggt ccaaaaattc gctcacagaa caaaccttct ttttcaggtt tcagtgttct
     4921 gtagttgatt gtttctggtt ttttaacttc cccgtaagac cagctacgga tcttatcagg
     4981 agaagccaaa gagatttgca tactttcaaa tttattaacg tctatcaagg ggccaacctc
     5041 cctttattct ttgagctgcc caaaatgctc tatctgcgtt tctcgtgagg caatgcgctt
     5101 tgatcaaaca tgcgcccgaa cgcttcagca gcgtccggga acacggatta gtctctcttc
     5161 attgcttcag attgtttatt taatctttcc atattcacaa catcgtcttc ttcatccatg
     5221 tcgcgcaatt cgatttcttc ttctgcttca tcaagcactt tcatatccaa tccgagtgct
     5281 tgcaattctt tcaccaatac gcggaatgat tcaggtacac ctggacgagg aattgtttcg
     5341 cctttgacga tcgcttcgta tgttttcaca cgaccgacaa catcatctga tttgtatgtc
     5401 aagatttctt gcaatgtata ggcagcacca taagcttcca gtgcccaaac ttccatctct
     5461 ccgaaacgtt gtccaccgaa ctgagcttta ccacccaatg gttgttgcgt aactaaagag
     5521 tatggtccag ttgaacgagc atgcaattta tcatcaacca tgtgggaaag tttcagatag
     5581 tacatgacgc cgacggatac acggttatcg aacggttgac ctgtacggcc atcataaagg
     5641 atggttttgg catcgttcgc cataccggct tcagccacag taccccaaac gtcatcttca
     5701 tccgcaccat cgaatactgg agatgcgacg tagatgccta atgaacgggc agccatacct
     5761 aagtgaagtt ccaatacttg tccgatgttc atacgcgatg gtacccctag agggttcagc
     5821 atgatgtcaa caggcgtgcc gtctggcatg tatggcatat cttcttcagg catgatacgg
     5881 gatacaaccc ctttgttacc atgacgtccg gccattttat ccccttcgtt gatcttacgt
     5941 ttttgaacaa tgtagacacg caccaacaaa ttcacgccag gtgacaattc atcccctgct
     6001 tcacgggtaa agatcttcac atcgtggacg ataccgccgc cgccgtgagg aacacgtagc
     6061 gatgtatcgc gcacttcgcg ggctttttca ccgaagatgg catgcaatag acgttcttcc
     6121 gcggacagtt ccgtcatccc tttaggagtg actttaccga ccaggatatc cccatctttg
     6181 acttccgcac cgatgcggat gatgccgcgc tcgtcaagat ctttcaaagc atcttcgccg
     6241 acgttaggca attcgcgggt gatttcttca ggtccaagct tcgtatcacg tgcttctgat
     6301 tcgtattctt cgatatggat agaagtgtac acatcatcac gcacaagacg ttcgttcatg
     6361 ataacagcat cctcgtagtt gtaaccttcc caagtcatga aggcaaccaa aacgttctgt
     6421 ccaagagcca tttcgccttt ttccatagac gggccatccg ctaacgtctc gccgacatcg
     6481 acacggtcac ccaaagctac gattggacgt tggttatagc aagttcctga gttggaacgt
     6541 tggaacttga tgatttcgta tttatccaaa gcaccattgt cgcgacggac acggatttct
     6601 ttggcatcca cgtattcaac cacaccatca ttttggcaca acagggctgc acctgagtca
     6661 tgggcggctt tgtattccat cccggtaccg acgaacggtg cttgcgggtt gatcagcgga
     6721 acagcttgac gttgcatgtt ggcacccatc aaagcacggt tggagtcatc gttttccaag
     6781 aacgggatgc aggctgtcgc gacagcaact acttgttttg gagaaacgtc catgtaatcg
     6841 acgcgttcga tcggaacttc caagttgtca tggatataac gggccatgac gatattgttg
     6901 gcgaagcttc cgtcttccaa taggaccgag ttggcttgcg ctacggtgaa gttatcttct
     6961 tcgtcagcag tcaagtagtc gatgatattg gtaaccttat gcgtgttcca gtcaacacgg
     7021 cggtaaggtg tttcaatgaa gccatatttg ttgattttcg cataactgga caagctgttg
     7081 atcagtccga tattcgggcc ctcaggcgtt tcgatcggac acatacggcc atagtgggaa
     7141 tagtgaacgt ctcggacttc atatccggca cggtcccgcg tcaaaccacc gggtcctaag
     7201 gcagaaagac gacgtttgtg cgtcaattcg cccaacggat tcgtttggtc catgaactgc
     7261 gacaattggg atgaaccaaa gaattctttg attgcagcca ctaccgggcg gatgttgatc
     7321 aattgttgcg gtgttacggt agaaacatcc tggatggaca ttctttcgcg cacgacacgc
     7381 tccatccgtg acaaaccgat acggaattgg ttctgcaaca actctccgac cgaacggata
     7441 cgtctgttac ccaagtggtc gatgtcatcc gttgttccca atccttcaga aaggttgaag
     7501 aagtagttca tggaagcaaa gatatcggca gtcgtcagat gtttgatatc gtcagcaatt
     7561 tcggaatttc cgatgatgtt gatgactttc tcagggtctt tcttagagta gactttcact
     7621 acctgcataa cgaccggatc agtcacaaca ccatcatcag tagggtacaa cgtcacttgg
     7681 ttcaagccgt tatccaaata aggcgctaag cgttccatta cgccgcgatc caattcggtt
     7741 cctttttccg cgatgatttc acctgtctca ggatcgacca atgtttcagc caatgtctgg
     7801 ttgtacaagc gtgttttgac attcaatttt ttatttactt tatagcgtcc aaccggagcc
     7861 atatcatatc ttcttgggtc gaagaaacgg gcattcagca agttacgcga gctgtcggcc
     7921 gttttcggtt cgcctggacg aagtctctcg tagacgtctt tcaacgcttc ttctgttctg
     7981 gagtctgatg cattcttatg tacatctttt tccatagtca ggcgtaagct ttcgttgtcg
     8041 ccgaagatct caagaatctg atcatccgaa ccgaagccca atgcgcggat caaaacggtc
     8101 aacgggattt ttctggtacg gtcgatacgg acgtaagaga tatctttcgc atccgtttca
     8161 tactccaacc atgcgccacg gttaggaatc aacgtgttgc cgaagctttc tttcccgttt
     8221 ttgtccatct tgttatggaa ataaacgcct ggagaacgaa ccaattgtga gacgataaca
     8281 cgttccgctc cgttgatgat gaaggtaccc atatctgtca ttaatgggaa atcaccaaag
     8341 aaaacttcct ggtctttgat ttcgcctgtt tctttattga tcaagcgtag ttttacataa
     8401 atcggcgccg agtagttggc atcatgtgca cgtgcttctg aaacagtata ttttggagca
     8461 tggaattcat aatcgacaaa ttccaatgct aagttccctg tgtgatcttc aatcggcgtg
     8521 atgtccttga acatctcttt gatcccggtt tccaggaacc atttgtaaga atccgtttga
     8581 atctcaatca ggttaggaag ctctaacact tcactgatac gcgaataact tctgcgggta
     8641 cgttgtttcc cgaatttttc attatgccct aacaacttct tcacccctac ttaaatttag
     8701 acctttagcg acaacacact cttatgttgc attttcgcaa caagtgttac gtttttgtta
     8761 catatacaat ccgcaacgtt tttttggcaa aaaaaaacca aaagaaggtt gcaatttctg
     8821 agaaaattac tctttctttt gttttctgcc ttgaaaagcg cgtctggaca atatttcagt
     8881 cgcgttttct gaattatctt atgcgtctat attaccttac tattctacac acatgatttt
     8941 catttgtcaa cagtctttcg ctcgcatccg atccgggaca cgtgctaggc tttgaccgat
     9001 tggatgatcc aatagccttt gtcgcgggca atgactgcga cattcccgaa gatatccaac
     9061 atcttttgct gagcactcgg cgcgccttgc ttcttttgga tgactacagt cagcgtgccg
     9121 cccggcaaga gatgcacgtg cgcttccgac aggatgcgat gaacgacctg tttaccggcg
     9181 cggatcggcg gattactgac gatagccgcg aattgccttt cttctggcac ttggtcgtag
     9241 gtattcgact cgtagacttt cacattgcgg atgttgttgg cttccgcatt tctgcgcgct
     9301 aaagacatag cccgctcgtt tacgtccacc atctccacca atcttgcagg ataggcatag
     9361 gcaagcgcca gccccatcgg accgtagccg cagccgacat ccaagatatc cccggcaacc
     9421 tcctcattca agcggaacgt ttcgatcagc aagcgcgaac cgaagtcgac cgtttttttg
     9481 gagaagacac cgctgtccga gatgaaacga aactcacggc ccctcaaagg gaaagtccag
     9541 gaagcttcct tgctttcggt ctcaggattc tttgtgtagt aatgatttga catatttcca
     9601 actccttctg acggaagtcc gacactgcag ggaaaataca agaaaagcca ggataattgc
     9661 ttatcctggc cagtctattt acacaatcaa gaattgtgtt ctgtaaaaca atcttttaaa
     9721 aaaagattat ttaactgtta cagaagcgcc aacttcttct aattgagctt tcaatgcttc
     9781 agcatcttct ttagatacag cttctttgat aacgccagga gcgccgtcaa ctagagcttt
     9841 agcttctttc aagcctaggc ctgtagcttc acgtacaact ttgattactt tgattttaga
     9901 atctccagct gaagttaatt caacgttgaa ttcagtttgc tcttcttctg ctgctgctac
     9961 tgcgcctgct gctgctactg gagctgcagc tgttacgccg aattcttctt cgatagcttt
    10021 tactaagtcg tttaattcta gaactgttgc tactttgatg tcagcaatga ttgcttcgat
    10081 gtttaatgac attttgtgtt cctccgtttt tggtttaagt ttttttgtgc ctccattaaa
    10141 gaggcttgta ttttggttca tctagcagaa ctgcaaatta tgcagcttcg ccgtctttct
    10201 cggatacagc tttaatagcc aacgcaaagt tgcggactgg agcttgtagt actgaaagca
    10261 acatagaaag cataccttcg cggtttggta gttttgccaa agcttcgatt tcttctttag
    10321 aagaaacgtt accctcaacg ataccacctt tgatttctag tttatcagca gtttttgcaa
    10381 attcagccat gattttggct ggagctacaa catcttcaga actgaatgca acagcagtag
    10441 gtcctttgaa gatgctttcc atgccttcca aacctgcagc gacagcagca cgtaccatta
    10501 gagagttttt gataactttc atttctacgc cagcttcacg caattgtttt cttaattcag
    10561 tcacctgttc aacagtaaga cctaaataat caacaacgac tacagatgcg gattcttgga
    10621 tttttgttgt cagttcttca accaacagtt gtttttgtgc aatagcagct tcactcatct
    10681 tatatttcac cccctggatt ttatgataga atttcttgta aaaacaaaaa actctatgtc
    10741 acctagacat agaggatcca taaatatctc attggattct tcctcggcag gtatattaag
    10801 gctcgcaccc cctgctgtct tcggtaagca tatattaacc tcaacgacta taccaaagat
    10861 agtcgctgag gtcaaggttt ttttataaag agcttgcgtc cactttaacg ccaggtccaa
    10921 atgtagttgt tacacttaca tttttaacgt attggccttt agcagttgct ggtttagctt
    10981 tcaagattac atcgtggatt gtacggaagt tttccatcaa tttagcatcg tcgaatgata
    11041 ctttaccgat aggaacgtgg atgttaccag ctttgtcagc acggtatgta actttaccag
    11101 ctttgatttc gtcaatagct tttgttacgt ccattgttac agtacctgtt ttagggtttg
    11161 gcatcaagcc tttaggtccc aatacacgac ccaaacgtcc aacttcaccc atcatgtcag
    11221 gagttgcaac gacaacatca aattcaaacc atccgccttg gattttttga accatatctg
    11281 cgtcgcctac gtagtctgcc ccagcagctt ccgcttcttt agctttttcg cctttagcga
    11341 acactaaaac gcgttgtgtt ttaccagttc cgtttggaag cacgactgct ccacggattt
    11401 gttggtcatt tttacgagtg tcgattccta aacggtaagc gacctcaaca gttgcgtcga
    11461 attttgcaaa atcgacttct ttcgctaatg ctactgcttc ttctactgaa tatgctttag
    11521 tagaatctac ttttttcgca gcatcttgga attttttact tttcttagcc attgaatttt
    11581 cctcctagat tgtggtttta acggattaac ctcccacgtg tggcccggaa caccttatga
    11641 gcgttcggaa ggccacatga gaagctgcat tttgaattag tcttgaactg taatacccat
    11701 tgaacgggca gtaccttcga ccatacgcat agcagcttct acgtctgctg cattcaagtc
    11761 taccattttt gtttcagcga tttctttaac ttggtcacgt gttacagtac caactttttt
    11821 cttgtttggt tcgcctgaac ctgattcgat acctgcagct tttttcagca atactggagc
    11881 tggaggagtt ttagtaatga aggtgaatga acgatcttca tatacagaaa tgacgacggg
    11941 aatgatcata cctgcttggt cagcagtacg tgcattgaat tctttacaga atcccatgat
    12001 atttacttgt gcttgaccca aagctggacc taccggtgga gcaggagatg ctttacctgc
    12061 aggaatttgt aacttaacta atttaacaac ttttttagcc acgagacata cctccttgat
    12121 tgttcgtgat gtggtttaat ggggttaaga tttcccctcc cactcaatcc atacgcctga
    12181 acgtaccgat atattatatc agtataccca aataaatgca atagtttttt tagcctttgt
    12241 cgacttggtt gtaatctagt tcggcaatcg tttcgcggcc gaacatttcg atcatgactt
    12301 tcaacttgcc tttttcgctg tccacttctt caacgaatcc tgacagaccg ttgaatgcgc
    12361 catcggtgat ggtgacgggc tcgccttttt caaagttcac atcatgcgta cgcgtgctca
    12421 tacccatacg cttcaggatg acttcgattt cttcattcaa taacgcagac ggcttgctgc
    12481 ccgctccgtg cgatccaacg aaaccggtaa cgcccggcgt gttacggact acgaaccacg
    12541 cttcatcaga cataatcatt tcgaccaaca cataaccagg gaaggttttt tgcgtagtcg
    12601 ccttctcttt tccgtccttc acttccactt gctcttcttc cgggataaca acgcggaaga
    12661 tatggtcttc catgttcatg ctttgcgcac gtgattcgat gttcagtttc actttgttct
    12721 catagcctga ataagtgtgc aacacatacc attgtttttc gttttcgatc gtttccattt
    12781 ttcgcatctt ccttcttggt ggatttttat tagacttcat ttattgtaaa caaaaaaacc
    12841 ttcctgtcgt tgaaaggttc cttctgtacg gcgttaacat tatagcatca cgaagactgt
    12901 atggctacaa atcttctttt taatttaaga ttaaactcat tgcttctgtg atcccaaaat
    12961 ctactacggc aaaaaatatt ccgaatagaa tggttgtaac gacaaccgta tttgtatatt
    13021 ttgctaattc cttaccagtc ggccaagtca cttgcttcat ttcatccgct acgctcttca
    13081 gaaatttcat gcttctcctc ctactatctg ttgccagttt cgtctttatt tcgtttcttt
    13141 gtgcaaggta tgcttcccac agtaccggca aaatttcttg acttccaaac gttctgtacg
    13201 acctttttca gtaggtgtga tggtatagtt ccttgatcca catacggtac aggctaatgc
    13261 ggcttttctg atagccatgt cgctcaccta ttttctctct atttgtgtac acaatacaag
    13321 ctaatattag agcgcctatc ccaaatagtc aaccctttta tttttgcgga tgcgccaaaa
    13381 atacaaaaag gcttccggat ctgagaccgg aaaccttttt gttgcgctat tcatttctgc
    13441 attaccttat ccgtgttgaa tcttacttct tattgaactg taacgattgc atcgccaaga
    13501 tctacagtac cttctgcaac aacttcaatg tttttgtaat tgaaagtgtt cgtgattacc
    13561 ataggagtcg ttgttttgta accggacgcc ttgatgaagt cgatatcgaa atcaaccaat
    13621 ttgtctccga tctcaacaag atctccagct ttaacatggg tagtgaaacc ttcacccttc
    13681 agattgactg tatccaatcc gatgtggatc aaaatttcaa caccttcgtc tgattcaaga
    13741 ccgatagcat gcttcgtgtc gaaaaccatt acgactttac cattgatcgg cgaaacaacc
    13801 tctccgactg caggttcgat tgcgacccct ttacccaatg cttcgctagc gaaggcagcg
    13861 tcatccactt gcgacaaggg aatagcggta cccgcgatcg gcgaacgtaa agtagtggca
    13921 actgtcgctt tttcttcaac agcaggtgct gctgttgttt caacaacttt ttcttctgtt
    13981 ttttcaacag ccttcagacc ataataatct tctttcgaag gcagttttgc ggcactgccg
    14041 ctttcgatga attcttccaa gtttgatttg atgttagaaa ctttcggtcc gtaaacaact
    14101 tgaacgccgt ttcctttatg gatgacgccg gatgctcccg tgcccttcaa taattccgca
    14161 tcaaccaaat ctccgtttgc tacggttgta cgcagacgtg tcgcacagct gtcgacgtcc
    14221 acaatgttcg ctgcgccacc cagaccataa atgatggaag cggacatttc atccgtctcg
    14281 ctcttcacga ctgaggcacc ctctgaagta ccttcttgcg ctttcgcatt catgtcggca
    14341 cgcgtataca acttcacttc agtcgaatca tcttcacgac ccggcgtctt gtaattgaac
    14401 ttcttgatca agaacgagaa caagaagtaa tagattacaa agtaagcaat tccgataaat
    14461 acgatccaaa tccagttcgt tttgacattc cct
//
LOCUS       DAOMUJ010000006        13118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1067_length_13118_cov_2.714690, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000006 DAOMUJ010000000
VERSION     DAOMUJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_13118_cov_2.714690"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLT73_00410"
     CDS             complement(<1..780)
                     /locus_tag="PLT73_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRM18874.1"
                     /translation="MIYEMTIEKNLLTEPISHTIRIEESSTFEQLYQDILQVLQEEAP
                     KNHSFQIMRSNGKKQSGVTIQPQPASDQVAEDPFVYNEDSENTSLFDERFYENGTPLK
                     DYFKKPGDAATYISVQDIPNEYTVTFSKTLTPAEAILAELNQLIERSPERGDSYDIIK
                     GQMDELTQLLNPSSRKSKKPAAPKQPQIYSFKITLENVRGPVWRKVQVNSASTFLELH
                     KIIQILFDWQDDHLHEFRIQKSNGIRQKNVVIESDEEASSPF"
     gene            1113..1403
                     /locus_tag="PLT73_00415"
     CDS             1113..1403
                     /locus_tag="PLT73_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18875.1"
                     /translation="MTRQKDQRMVQSRFICQECGAQMVLYRKRAEQRKLGHVKHMYCP
                     SCKETKAFTEIKDWDRNVVFWEDYQNALLTEAETAELWDALWARSSESLLHG"
     gene            1434..2504
                     /locus_tag="PLT73_00420"
     CDS             1434..2504
                     /locus_tag="PLT73_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18876.1"
                     /translation="MMDEDNIFESHEFEAKADFEMNWEDLLEELSDYEIPEERLSELA
                     AQDGGFPFLELHFQELRYLNLMDEQLYVGENESAEFYTSMANTYGTRVIDGIEYALMD
                     DFAYHYFHEMMTVEAVDRQGRQFVMVYGMPDNWEEIEKQVEEKVWDPSEEDEVAAADG
                     TETWEHPDMVISMIDFRNEIINRAALELRKNGFPLDLNFQSKRMFMDDVLISRAITLQ
                     MPGIIAAFEKISANFSDVDHIGFSMDEKRIRIFYADTDETIDHMLSACGKIANGSAKK
                     TGIGEVFYDTFLDRVERALFELIFLVNQSTDELSFVSGVAVQRLDAIRAGELALSEMT
                     LDEINRLMLSFPELLNLEWLFV"
     gene            2880..4043
                     /locus_tag="PLT73_00425"
     CDS             2880..4043
                     /locus_tag="PLT73_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM18877.1"
                     /translation="MNYVFISPHFPSNFKNFAVGLKKAGVHVFGIASEPYELLDTDLR
                     ESLTEYFRVNDMENYDEMLRAVAYLTYKHGKMDRIESHNEYWLAQDARLRTDFNVFGF
                     KNEDMAFVKLKSAMKEKFKEVGIRVARGRVIGDYQDALGLCHEYGFPVCIKPDSGVGA
                     ADTHKIRSEEELGHFFHVKNPYESYILEEFIDGKIVTYDGLTDANGNILFDATLVYEK
                     AVLEIVEKNADMFYYVDRYMPDDLREIGTKLLQAFNVRERFFHFEFFRLPDGSLVALE
                     ANLRPPGGHTMDMWNWANDVDLYAAYGELVATGNFTAQMHFPYYCCYVSRKGVHPYRF
                     THEEVMARYGGNVVLFEEMPGIFAAIMGDFGYVIRAAEYQELMDIIHMISVTE"
     gene            4059..4811
                     /locus_tag="PLT73_00430"
     CDS             4059..4811
                     /locus_tag="PLT73_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002272033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM18878.1"
                     /translation="MHVEYSSQWSGHLNREMRFNRYGHAGKPIIVFPSSGGSYNEYAD
                     FGMIEACQWFIDQGLVQFYTPDSVDNESWLCEWKSPYDRAHMHERYDRYIIEELVPHI
                     KHQTNHQGPMIATGCSMGGYHSLNFYLRHPDAFDTVIALSGLYDVRYFFGDYHDRNVY
                     ENSPIDYLWNLNDGWFLDKYRSGNIIVATGQGNWEEVSIKDTKKIEEALRFKSIPAWI
                     DFWGENVHHDWEWWRVQMPYFLGKLNEQGKLN"
     misc_binding    5221..5480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5533..6579
                     /locus_tag="PLT73_00435"
     CDS             5533..6579
                     /locus_tag="PLT73_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRM18879.1"
                     /translation="MTETNRKQLIFDTFDNKETERVPVGFWFHFVFGDAQFRGLEDRS
                     ILDRVIAGHKEFYDAFKPDFVKIMSDGFFGHPSLLEKRIEIADDLYNVQAVGPNHPWI
                     TEQVKTVKRIKESFNGEVATFYNIFSPANYIRIAFEAWDKDPEKFTRFFETEPEALAY
                     AANEIAKDIAVLTQRVIEEAGTDGIYLSVQNVQSSTATKEAYQNYIAPSELTVLAEAN
                     KVSDYNILHICGYEHHQNDLSYYRDYEAKAYNWAVHTEKISLKEGKDFFGNKAVIGGF
                     DNNTGTLLDSGNKADIARFTVDIIHEIGDTGVIIGADCTVDPQIGLDRLELVRQTAAG
                     VSGKNNKQTTSLQK"
     gene            6730..7770
                     /locus_tag="PLT73_00440"
     CDS             6730..7770
                     /locus_tag="PLT73_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM18880.1"
                     /translation="MVGIVIIGCGDVSKQRHAPLSDKNEKVDLVGFYNRTIAKAEAFQ
                     KKYGGKVYRTLEEIWEDGTVDAVIVATNEQSHSPITIAALEAGKHVLCEKPMADSVAE
                     AERMQQTAEKTGKKLMIIHNQRLYPAHQLLKKLLTEGTLGKIHAYRTTLANQGQENDG
                     WGTAVPDFYDRIGHTNGALAQVGVHRIDLLNYLFSEDPVTAVLAQTATQGKQLADGSP
                     VPYEDYAVLQLQHRSGIQGTLLTHWFDYSNERQTVIYGEKATAVTYADGHPVALYRKN
                     GEKTYLDDPSQDGFYAEPLTPIVDRFVESIEQDTVPFVTAEQGITALRILAAAYRSQA
                     IQTWIPIEQEDA"
     gene            7772..8875
                     /locus_tag="PLT73_00445"
     CDS             7772..8875
                     /locus_tag="PLT73_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRM18881.1"
                     /translation="MTETFNGRKRIEAALKKQPLDRPAISGWRHMPLVDRDVEAFAEA
                     TIAFTDENDWDLIKLMSQGQFFAEAYGTPITFSTDAKEWAGKILDYPIRSAEDLTRLV
                     PLKADNPVIQREAAFAKKIVKHYKGEKVVVGTVFSPLSWVKQFAPGNDSIAHIVDGPE
                     ESPIAEFLEHHREELKAALDVLHASNKVFVDELIAAGVDGFFIAEQYSQRGDISEADY
                     AAFAKPYTDDLLAYIKDRTWFNILHAHGHVDLAVERFIDYDVQAINWEDGSDHAEDPT
                     RLSFRKLRALTDKLLVGGISPKTDLTFSNQAQEIEDLLVDRLADVLTETGDTGIVFAP
                     GCAISLEANPDFYQRIHAAAQKVQATILKTANH"
     gene            8909..9781
                     /locus_tag="PLT73_00450"
     CDS             8909..9781
                     /locus_tag="PLT73_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM18882.1"
                     /translation="MKRKRLVTTVSLLGAAFLAGCGQIGAETSSEATSGSAAEAVEVT
                     TVKVAHTQSSAPMNYVDENGESTGYEVSVLKAIDELLPQYEFEYVPTSDEDLLIGIES
                     GKYAVGTKGAWYTEERAQKFIFPEHYIGASSIGVLIRSEDAAEITDLETFAAAGKSLV
                     PISPNNAQYNVIQAFNEEHPDTPIELTAADVFEIADAYTWLLEGRYDGYFAVKTAYEV
                     NVLAEGAPYSQYKDDFSYFVYEALPTWPLFNKDQQALADAYDEAFLQLQEDGTIDDLM
                     IEFLGENTFQYVKE"
     gene            9843..10553
                     /locus_tag="PLT73_00455"
     CDS             9843..10553
                     /locus_tag="PLT73_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM18883.1"
                     /translation="MNRPFDITYIWKAVPDILSGLGVTLQFLIGTIVIGGILGFLATW
                     ARLRGPKWLRAIVHSYIWIIRCTPSIVMMFLVFYGLPEVLLGVFGIDINSWNRLYFVV
                     IALSLLFSAPCSEIMRSAYLAVAKVQRETALSIGLTEAQAFRRIVLPQALVSALPNLG
                     NSFILLMKEGALAYTIGIVDIMGKGQLIIARNLGGYSIETYIALAGIYWLLTLAIEKA
                     FKLAEDKLSAGKQPLRAA"
     gene            10572..11363
                     /locus_tag="PLT73_00460"
     CDS             10572..11363
                     /locus_tag="PLT73_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM18884.1"
                     /translation="MTIDLAYVQEILILVLKGIPTTLRLTFIPLLLALPFAFLIAVAR
                     QRNLKVLARVSQVYVSFIRGTPLIVQILVMYSIFPSLLNVFVKSRGIDFDVFGINPIY
                     YAYIVFTLNTIAILSEVFRSALETVDKRQLEAALSIGLLPIQAYRRIVIPQALVAATP
                     NLCNSTVNLLKSTSLAFLMSVQDITAIAKTEAAFGYNYLESYLVIFIVYIVLCSLVQV
                     FFQLLEQRLSRYKRGRNIAVKSENQPEYFIDARKERYHAGIEEYS"
     gene            11338..12078
                     /locus_tag="PLT73_00465"
     CDS             11338..12078
                     /locus_tag="PLT73_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM18885.1"
                     /translation="MLELRNIHKKFGELEVLKGVDIDVEKGDIVVILGPSGSGKTTLL
                     RCINFLERADEGSLRFDEIQTEFSKASKKDINAVRKKSAFVFQNYGLFDNKTVLDNVT
                     EGLIVGRKVPKKEAIEAAIRALEKVGLSDRLAYYPSQLSGGQQQRVGIARALAVNPDV
                     ILFDEPTSALDPELVGEVLQVIRKLAEEGTTMVIVTHEMTFAKEVANRVIFMDQGVIV
                     EEGAPEAIFGNPKEARTRGFLQRILARA"
     gene            12295..>13118
                     /locus_tag="PLT73_00470"
     CDS             12295..>13118
                     /locus_tag="PLT73_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM18886.1"
                     /translation="MLKDKWVITTTLAISLVLAGCSTGDAANTESSEGAAASSATPAA
                     EQVITITNTGELASLDNAISADNSSLVVLENANEGLYRVGDDGAFELGIAAEEPVVSE
                     DGLEYTFKIREDANWSNGDPVTAQDFVYTYQKIVDPATASPNINKLYPLKNAEAINNG
                     ELDKAELGVEAIDEKTVKFTLGNPTAYFKDLLGTSSYLPQNHQVASELGSAYGTNSEN
                     TVYNGPFVVEGWEGTDLNWSMVPNEAYWDAENVKLDQINWEVSKEIATNINLFESG"
BASE COUNT         3422 a         3044 c         3542 g         3110 t
ORIGIN      
        1 gaagggggat gaggcttctt catcagattc gatgacgacg tttttctggc ggatgccgtt
       61 cgatttctgg atcctgaatt cgtgcagatg gtcatcctgc caatcgaaca ggatctggat
      121 gattttgtgc aactccagga aagtgctggc gctgttgacc tgaaccttcc gccagaccgg
      181 gcctctgacg ttttcaagcg tgatcttgaa ggagtagatt tgtggctgct taggtgcggc
      241 tggctttttc gattttcggg aggatggatt caatagctgt gttaattcat ccatctgccc
      301 ttttataatg tcatagctat ctccgcgttc gggagaccgt tcaatgagtt ggttgagctc
      361 tgccaagata gcctctgcgg gagttaatgt tttgctgaaa gtgactgtgt attcatttgg
      421 aatatcctga acggaaatgt acgttgccgc gtcaccgggt ttcttgaagt agtccttgag
      481 gggtgtgcca ttttcataga atctttcatc gaaaagcgag gtattctcag agtcttcgtt
      541 gtaaacaaag ggatcttcag ccacttggtc agaggcaggt tgcggctgaa tcgtgacgcc
      601 ggactgcttt tttccgttgg agcgcatgat ctggaaagaa tgatttttgg gagcctcttc
      661 ctggaggact tgcaaaatat cttgatatag ttgttcgaat gtggaacttt cctcaatccg
      721 gattgtgtgt gaaatcggtt cggtcagcag atttttttct atcgtcattt cgtaaatcac
      781 gtaagagcct ccttggcaaa aataattgcc gcaatttggg tgttagtatc cctatcataa
      841 ccaataaggg gagagggaag caacttgata ttttgcggat gagcatacca gaaaatagtt
      901 atatcagtat aaaaagttgc cgtttaagcg gccgtatcaa ggggcatgaa agtcaatttt
      961 gtgaaatatt gacgacgaat tcacaaaatt gtttcagtgt gtgtctaggt gtatgatagg
     1021 tataacgaaa ggttgcaatc gtttcgtata cccatttgtt ttttgagcac tgtcccttcg
     1081 gatttgtcat tcagaggaat ggagtgttga aaatgaccag acaaaaagat cagagaatgg
     1141 tccaaagcag gttcatctgc caggaatgcg gagcgcagat ggtactttac cgcaaaagag
     1201 ccgagcagcg caaattaggg cacgtcaaac acatgtattg cccgagttgc aaggaaacaa
     1261 aggccttcac ggaaatcaag gattgggacc ggaatgttgt tttttgggaa gattatcaaa
     1321 atgcgctatt gaccgaagcg gaaacagccg aactctggga tgcactgtgg gcgcgcagct
     1381 ccgaatcgtt gttgcatggc tgaatgggag acgtgctgaa agggaggcgg acaatgatgg
     1441 atgaagacaa tatttttgag agtcatgaat ttgaggcaaa agctgatttt gaaatgaact
     1501 gggaagactt gttggaagaa ctgagcgatt atgaaatacc tgaagagaga ttgagtgaat
     1561 tggctgctca ggatggtggc tttccgttcc tggaactgca tttccaagaa ctgcgctatc
     1621 tgaatttgat ggatgaacag ctctatgtcg gagaaaacga atctgcagaa ttctacacaa
     1681 gcatggcgaa cacatacggc accagggtaa tcgatgggat cgaatatgcc ctcatggatg
     1741 acttcgccta tcattatttt catgaaatga tgacggttga agcggtggac cgccaaggca
     1801 ggcagttcgt catggtctac ggcatgcccg ataactggga ggaaattgag aaacaggtag
     1861 aggaaaaggt ctgggatccc agtgaggagg atgaagtggc ggcagctgat ggaaccgaga
     1921 cgtgggaaca cccggatatg gtcatcagca tgatcgattt ccgcaacgaa atcatcaatc
     1981 gggcagcgct tgagttgcga aaaaatggct tcccattgga cctgaatttc cagtccaaac
     2041 ggatgttcat ggacgatgtg ctcatatcga gggcgatcac acttcagatg ccgggcataa
     2101 tcgcggcttt tgaaaagatc tccgcaaact tcagtgatgt cgaccacatt gggttttcga
     2161 tggatgagaa aaggatccgc attttttacg ccgacacgga cgaaacaatc gaccatatgc
     2221 tttcggcttg cggaaagata gcgaacggat cggcaaaaaa gaccggaatc ggtgaggttt
     2281 tttatgatac tttcttggat cgggtcgaac gcgcattgtt cgaactgatc ttccttgtca
     2341 accaatcgac tgatgaattg tccttcgtga gcggggtcgc tgtgcaacgg ctggatgcaa
     2401 tccgggcagg cgaactagcg ttgagtgaaa tgacgttgga tgaaatcaac cggttgatgc
     2461 tcagctttcc ggaactgttg aatttggaat ggctgttcgt ttaagaaagt gaggttgtta
     2521 acgctcaatc aatcccctta gagcttcgtc gtattcagcc atggattcaa tcacacatgc
     2581 taaaaatgtt tccgaatcat tcgttttctg attttgttgt cgggaaagaa gggtgcttcg
     2641 cgcatctgat gagagtcgaa tgatcctggc ggatgctctt ttggctgaga accgatttat
     2701 ctttttcata ctgttcacgc ctttcgaaga aatggatttt agttattctt agcttatcag
     2761 aagaacctca cgattgtatc aatatttttc gttttcacca gaaaaacgct tgtaaatctg
     2821 ccgttataat ggtacattct cattataatc taatgttcag aagagagaag ggaatcagca
     2881 tgaattatgt atttatttca ccccattttc ccagtaattt caagaatttc gcggtcggtc
     2941 tgaagaaggc gggcgtgcat gttttcggga tcgccagcga accttacgag ttgttggata
     3001 cggacttgcg cgagagtttg accgagtatt tccgcgtcaa tgatatggaa aattatgatg
     3061 aaatgctgcg cgcggtggct tacttgacgt acaagcacgg caaaatggac cgtatcgaat
     3121 cgcacaatga atactggttg gcgcaggatg cgcgtctgcg caccgatttc aacgtgttcg
     3181 gcttcaaaaa tgaggatatg gccttcgtaa aactgaagtc cgccatgaaa gaaaaattca
     3241 aggaagtcgg tatccgggtg gcacgcgggc gtgtcatcgg cgactatcaa gatgcccttg
     3301 ggttatgcca cgaatacgga tttccggtct gcatcaagcc ggattccggt gtcggtgcgg
     3361 cggatacaca caaaatccga tccgaagagg aactcgggca ctttttccac gtgaagaacc
     3421 cttatgaatc ctacattctc gaggaattca tcgacggaaa gatcgtcacg tatgatggct
     3481 tgaccgatgc aaacgggaat atccttttcg atgctactct ggtgtacgaa aaagccgtgt
     3541 tggaaatcgt cgaaaaaaat gcggatatgt tctactatgt cgaccgctat atgccggatg
     3601 atttgcgtga aatcggcacc aagctcttgc aggctttcaa tgtccgcgag cgtttcttcc
     3661 attttgagtt tttccggtta cctgacggca gcctggttgc cttggaagcc aacctgcgtc
     3721 cgcctggcgg gcatacgatg gacatgtgga actgggcgaa tgacgtcgat ctctatgccg
     3781 cttatggcga actggtcgcg accggaaatt tcacggcgca gatgcatttt ccttattact
     3841 gctgctacgt cagccgcaag ggcgtccatc cttaccgctt cacacatgag gaagtgatgg
     3901 cccgttacgg tggcaacgtg gtcctgtttg aagaaatgcc ggggattttt gcggctatta
     3961 tgggggattt cggctacgtg atccgtgcgg cagaatatca agaactgatg gacatcatcc
     4021 acatgatttc cgtaaccgaa tagactgggg gaaaaaagat gcacgtagaa tattcatcac
     4081 aatggagcgg ccatttgaac cgcgaaatgc gtttcaatcg ctatggacat gccgggaaac
     4141 cgatcatcgt cttcccatcg tctggcggca gttacaacga atatgcggac ttcggcatga
     4201 tcgaagcctg ccagtggttc atcgaccaag gcttggtgca gttttatacg ccggacagcg
     4261 tcgacaacga atcctggctg tgcgaatgga aaagtccgta cgaccgcgcc cacatgcacg
     4321 aacgctatga ccgctacatc atcgaggaac tggtgccgca catcaagcat cagacgaacc
     4381 atcagggacc gatgattgcg accggctgca gcatgggcgg ctaccacagc ctgaactttt
     4441 acctgcgcca tccggatgct tttgataccg tcattgcact gagcggtttg tatgacgtgc
     4501 gttatttctt tggggattac cacgaccgca acgtctatga aaattcgccg attgactacc
     4561 tgtggaatct gaacgacggc tggttcctgg acaaataccg ttccggcaac atcattgtcg
     4621 ccaccggcca aggtaactgg gaagaagtca gcatcaaaga cacgaagaaa atagaagaag
     4681 cgctgcgctt caagagcatc cccgcctgga tcgatttctg gggtgaaaac gtccaccacg
     4741 actgggaatg gtggcgcgtc cagatgccgt atttcctggg caaactgaac gagcaaggca
     4801 aattgaattg atgaaaaatc tgtccttccg gcattttacc gggaggactt tttgttgcgt
     4861 gacgatggat gttgtggggg gcgggccgtc cgtctttttt gagcgtaaac atgaataact
     4921 agccgttatc catctttttc gtgaggcggc ggctcgaaaa gatggattca taaatattat
     4981 tcatctttta tgtaggtcaa acattcgaaa agatgaatac tttcgcggta ttcatctttt
     5041 tgggcccttt tcgcattcga aaacatggat acttggctct tatccatgtt ttccccaagc
     5101 atgcatactc aaccggccac tccactgttc gtttttccct gtcaatcctg ttcggaatcg
     5161 tgtgcttttc aggccgtggc tgcggtggta gactttgtct atcaaataaa agccatacag
     5221 aaaaaagcaa agaagaggaa agtactggtt ggatagtcct tcagagagct cttggtcggt
     5281 ggaaaagagc ggacggcagc acaggaaaaa tggcctcgga gttgatgcgt tgacagggat
     5341 tttcctgcag acgtatccgg acgcccccgt tagtcaggca acggtatcga gcgcagcaaa
     5401 aggctctgta ccggttgagg cggcaagcgc aagcttttcg cgaaaaaagg tggaaccacg
     5461 atctaacagt cgtcctttct ggaacaatcc aggaagggcg gctttttatt ttacatttta
     5521 ggaggaaaac atatgacaga gacgaataga aaacaattga tttttgacac tttcgacaac
     5581 aaggaaacgg agcgtgtgcc ggttggcttt tggtttcact ttgttttcgg tgatgctcaa
     5641 ttccgtggtt tggaggacag atcgattttg gatcgagtga tcgcggggca caaggaattc
     5701 tatgacgcct tcaaacccga tttcgtaaag atcatgagcg atggcttttt cgggcatccg
     5761 agtctgttgg aaaaaaggat tgaaatagcg gatgacctct acaacgtcca agcagttggg
     5821 ccgaatcacc cttggatcac ggagcaggtc aagacggtga aacggatcaa ggaatccttc
     5881 aacggcgaag tcgcaacttt ctataatatc ttttcgccgg cgaactatat ccggattgcc
     5941 ttcgaagcgt gggacaagga tcccgaaaaa tttacccgct tcttcgaaac cgaaccggaa
     6001 gcccttgctt atgcggcgaa tgaaatcgcc aaggatatcg cagttttgac ccagagagtc
     6061 attgaagagg cgggcacgga cggcatctat ctgagcgtgc agaacgtcca aagcagcact
     6121 gccaccaaag aagcctatca aaattatatc gcgccgagtg agttaacggt attggcggag
     6181 gcgaacaaag tcagcgacta caatatccta catatctgcg gctatgagca tcatcaaaac
     6241 gacttgtcgt actaccggga ttatgaagca aaagcctaca actgggcggt acacaccgaa
     6301 aaaatcagcc tgaaggaagg caaagatttc ttcggcaaca aagcggtgat cggcggcttc
     6361 gacaataaca ccggcacatt gttggactcg gggaacaagg cggacatcgc ccgattcacg
     6421 gtcgacatca tccacgaaat cggcgataca ggcgtcatca tcggagcgga ttgcactgtc
     6481 gatccgcaga tcggactgga tcgtttggaa ttggtaaggc aaacagcggc aggcgtctca
     6541 ggcaaaaaca acaaacaaac gacgtcgctg caaaaataaa acaggacggc aggagcggtg
     6601 ttcgccaaag tcggtcaacc ctgatagaca atcggtatta tttttaaaat aagcaccgtg
     6661 ttagaatcag acacatactt aaaaacgata gccattccgg taagccggag ttcaggaagg
     6721 aggggtccca tggtcgggat cgtcatcatc ggttgcgggg atgtttcgaa acagcggcac
     6781 gcccctctaa gcgataaaaa cgaaaaagtc gatctggttg gattctataa tcgtacgatc
     6841 gcaaaagcag aagctttcca aaagaagtac ggcggaaaag tctatcggac tcttgaggaa
     6901 atctgggaag acggaacggt cgatgccgtt atcgtcgcaa cgaacgaaca atcgcacagc
     6961 cccatcacca tcgctgcact ggaagccggt aagcatgtgc tgtgtgaaaa gccgatggcg
     7021 gattcggtgg ccgaggccga acgcatgcag cagacagccg aaaaaacggg gaaaaagctg
     7081 atgatcatcc ataaccaacg actttatccc gcacatcagc tgctgaaaaa gttgctgacg
     7141 gaagggacac tcggaaagat tcatgcttac cggacgacat tggccaacca aggtcaggaa
     7201 aatgacggtt ggggtacggc ggtccccgat ttctacgatc ggatcggcca tacgaacggc
     7261 gctctggcgc aggtgggcgt ccaccgtatc gatttgttga actacttatt cagcgaagat
     7321 cccgtcactg cagtgttggc ccagaccgcg acacaaggca agcagttggc ggacggaagc
     7381 ccggttcctt atgaggatta tgcagtgttg caactccaac atcgctcggg catccaagga
     7441 accttgctga cccactggtt tgattattcg aacgaaaggc agaccgtcat ctacggcgaa
     7501 aaggccacgg ccgtaactta tgcggacggg catccggtgg cactttaccg gaagaacggg
     7561 gaaaagacct acctggatga tccctcgcag gacggcttct atgcagaacc attgacaccg
     7621 atcgtcgaca gattcgtcga aagcatcgaa caggatacag ttcccttcgt gacagcggaa
     7681 caggggataa cggcattgcg tattctggcc gccgcttacc gctcgcaagc aatacagaca
     7741 tggataccca tcgaacagga ggatgcataa catgacagaa acatttaatg gaagaaaaag
     7801 aatagaagcg gcactcaaga agcaaccttt ggacagaccg gccatttccg gttggcgtca
     7861 catgccgttg gtggatcggg atgttgaggc gttcgccgaa gcgacgatcg ccttcacgga
     7921 tgagaatgat tgggacctga tcaagctgat gtcgcaaggc caattcttcg ctgaagccta
     7981 cggaacgcca atcaccttct cgacggatgc gaaagaatgg gcaggcaaaa tactggacta
     8041 tccgatccgg agcgcggaag atttgacccg tttggtgcct ctcaaggcgg acaatccggt
     8101 gatccagcgc gaagccgctt ttgccaagaa aatcgtgaag cactacaaag gcgaaaaagt
     8161 ggtcgtcggt accgtcttct cgcctttgtc atgggtgaag caatttgcgc cgggtaacga
     8221 ttcgatcgcg catatcgtcg acggaccgga agaaagcccg attgcagaat ttctggagca
     8281 tcaccgtgag gaattgaagg cggcgctcga tgtgctgcat gcctccaaca aggtgttcgt
     8341 ggacgaattg atcgctgccg gcgtggacgg cttcttcatc gccgaacagt acagccagcg
     8401 cggcgacata tccgaagccg attatgccgc tttcgccaaa ccttatacgg atgacctgtt
     8461 ggcttatatc aaggaccgca cctggttcaa tatcctgcat gcgcatggac atgtcgattt
     8521 ggctgtggag cgttttatcg attacgatgt ccaagccatc aactgggaag acggcagcga
     8581 tcacgcggag gatccgaccc ggttaagttt ccgcaaacta cgtgccctga cggataaact
     8641 gctggttggc ggtatctcgc cgaaaaccga tttgacgttc tcaaatcagg cgcaggaaat
     8701 cgaggacttg ctggtcgatc gcttggcgga tgtcctgact gaaaccggcg acacgggcat
     8761 cgtatttgcg ccgggctgcg cgatttcatt ggaagccaac ccggattttt accaacgcat
     8821 ccatgcggct gcccaaaaag tgcaggctac catcctaaaa acagcgaacc attaaacgaa
     8881 tcagaaacat acaggaggag taaattatat gaagagaaaa agattagtga cgacagtaag
     8941 tttattgggg gcggcatttt tggctggatg cggccagatc ggagcggaaa cttcaagcga
     9001 agcaacgagc ggttctgcag ccgaggcggt ggaagtgact acggtgaaag tcgcgcatac
     9061 acaaagttcc gcaccgatga attatgtcga tgagaacggc gaatcgaccg gctatgaagt
     9121 cagcgtgctg aaggccatcg acgagttatt gccgcagtat gaattcgaat atgtgccgac
     9181 ttccgacgag gatctgctga tcgggatcga atccggcaag tatgctgtcg gaacgaaagg
     9241 cgcttggtac accgaggagc gcgcacagaa attcattttc cctgaacatt acatcggcgc
     9301 cagcagtatc ggcgtgctga tccgttcgga agatgcggct gaaatcacgg acttggaaac
     9361 attcgcggct gccggcaaga gcctagtgcc gatttcgccg aacaacgccc agtacaatgt
     9421 gatccaagca ttcaatgaag agcacccgga tacgccgatc gagttgactg cggcagacgt
     9481 attcgaaatc gcggatgcct acacctggtt gttggaaggc cgctatgacg gctacttcgc
     9541 cgtcaagacc gcttatgaag tgaacgtgct ggcagaaggc gcaccgtaca gccaatacaa
     9601 ggatgatttt tcctacttcg tctatgaagc gctgccgaca tggccgttgt tcaacaagga
     9661 ccaacaagcg ttggccgacg catacgacga agccttcctg caattgcaag aagacggaac
     9721 aatcgatgat ttgatgatcg aatttcttgg cgaaaacaca ttccaatacg tgaaggaata
     9781 agattcgcgc tgctgccgga gaacggaaca caacagctga agctacaaag ggaggcaaaa
     9841 agatgaatcg tccattcgac atcacttata tttggaaggc agtgccggac attctgtccg
     9901 gtctcggcgt gacgttgcaa tttttgatcg gaacgatcgt gatcgggggc atcctgggat
     9961 ttttggcgac ctgggcaaga ctccgcggtc caaaatggct gcgggcaatc gtccatagct
    10021 atatctggat catccgctgc acgccttcga tcgtcatgat gttcttggtc ttttacggtc
    10081 tgccggaggt gctgttgggt gtcttcggca tcgacatcaa tagctggaac cgcctctact
    10141 ttgtcgtcat cgccctgtcg ctcctgttct ccgcaccttg ctcggaaatc atgcgttcgg
    10201 cttacttggc ggtcgctaaa gtgcaacgtg agactgccct cagcatcggc cttacggaag
    10261 cgcaagcgtt cagaagaatc gttttgcccc aagcgctcgt gagtgcgttg ccgaacctcg
    10321 gaaattcgtt catcctgttg atgaaggagg gggctttggc ctacaccatc ggcatcgtcg
    10381 acatcatggg caaaggccag ctgatcatcg cgcgcaatct cggcggatat tcgatcgaga
    10441 cctacatcgc cttggcgggc atctattggc tattgacgct ggcgatcgaa aaagcgttca
    10501 agttggcgga agacaaacta tcagcaggaa aacaaccgct gcgcgcggcc taagagaaag
    10561 gagcaagacg catgaccatt gatttagcct atgtgcagga aattctgatc cttgtgttga
    10621 agggcatccc gacaacactg cgcttgacct ttatcccctt gctgctcgcg ttgccgttcg
    10681 ctttcctgat cgcagtggca agacagcgca atctcaaagt actggcccgt gtttcccaag
    10741 tttatgtgtc cttcatcaga ggaacccctt tgatcgttca gattctggtg atgtacagca
    10801 tcttcccgag cctgttgaat gtgttcgtga aaagcagggg aatcgatttc gacgttttcg
    10861 gcatcaatcc gatctactac gcctatatcg tctttacgct gaatacgatc gcaatcctgt
    10921 ccgaagtctt ccgttcggcc ctcgaaacgg tcgacaaacg tcagctggag gcggcccttt
    10981 cgatcggcct gctgccgatc caggcctacc gtcgcatcgt gattccacaa gcattggtgg
    11041 cggcaacgcc gaacttatgc aacagcaccg tcaatctgct gaaatcgacc tcgctggcct
    11101 ttctgatgtc ggtccaggat atcacggcca tcgccaagac ggaagcggca ttcggctaca
    11161 attatctgga gtcgtacctt gtgattttca ttgtgtacat tgtgctctgt tcactcgttc
    11221 aggtgttttt ccaattgttg gagcagcgct tgtcccgcta caaacgcggg cgaaacattg
    11281 ccgtaaaatc agaaaaccaa ccggagtatt ttatagatgc gcgaaaggag cgataccatg
    11341 ctggaattga ggaatattca taagaaattc ggtgaactgg aagtcttgaa aggtgtggat
    11401 atcgacgttg aaaagggcga tatcgtcgtg atcctgggtc caagcggttc gggtaagacg
    11461 accttgctgc gctgcatcaa cttccttgag cgagccgacg agggttcgct gcgtttcgat
    11521 gagattcaga ccgaattttc gaaagcaagc aagaaggaca tcaatgcggt gcggaaaaaa
    11581 tctgcattcg ttttccagaa ttacggcctg ttcgacaaca agacggtatt ggataacgtc
    11641 actgaaggcc tgatcgtcgg acgcaaagtt cccaaaaaag aagccatcga agcggccatc
    11701 cgcgccttgg aaaaggtcgg gctgagcgac cgcttggcct actatccttc gcaactatct
    11761 ggaggccaac agcaacgggt gggcatcgcc cgcgccctcg cggtcaaccc ggacgtcatc
    11821 ctgttcgacg agcccacttc ggctttggat ccggaacttg tgggcgaagt gttgcaagtg
    11881 atccgcaaac tggcggagga aggcaccact atggtcattg tcacgcatga aatgaccttc
    11941 gccaaggaag tggccaaccg cgtcatcttc atggatcagg gggtcattgt ggaggaaggc
    12001 gcccctgaag ctattttcgg caatccgaaa gaagcccgca ccagagggtt cctgcagcgg
    12061 attttggccc gcgcttgatc taactggcag ctcccttatt tagctcgtcc taccgttcgc
    12121 aacgggggcg agctttttgt ggttattgga agtatcggcg ttgcctatca cagttgttcg
    12181 gaaatcggaa ttatcctaaa gcatcctgtg atggtaagct acttgtacat acatatcaaa
    12241 gaaatgaatt ccaacatagg gataaaaata atgagaatcg tcaggaggaa cagtatgtta
    12301 aaagataaat gggtcatcac gacaacgcta gcgataagtt tggtgttggc gggctgttcg
    12361 accggggatg ccgcaaatac ggaaagcagc gaaggggcag ccgcttcatc agccacacct
    12421 gccgcggagc aagtcatcac tatcacgaat acgggggaat tggcttcctt ggacaacgcc
    12481 atctcagcag ataactccag cttggtggtg ctcgagaacg caaatgaagg tctttatcgc
    12541 gtcggtgacg atggcgcgtt tgaattgggt atcgccgccg aagagcctgt cgtcagtgaa
    12601 gacggactgg aatatacttt caagattagg gaagatgcga actggtcgaa cggtgatccg
    12661 gtgactgcgc aggatttcgt ctatacctac caaaaaatcg tcgatccggc gacggcttcc
    12721 ccgaacatca ataaactgta cccgttgaag aacgccgaag cgatcaacaa cggggagttg
    12781 gacaaagcgg aactgggagt cgaagcgatc gatgagaaga ccgtgaagtt cactttgggc
    12841 aatccgaccg cttatttcaa ggatttgttg gggacgtctt cctacttgcc gcagaatcac
    12901 caagtggcga gcgagttggg cagcgcctac ggaacgaaca gtgaaaacac ggtgtacaat
    12961 ggacctttcg tcgtggaagg ttgggaaggc acggatctga actggtcgat ggttccaaac
    13021 gaagcttatt gggatgcgga aaacgtcaaa ttggatcaga tcaactggga agtcagcaaa
    13081 gaaatcgcca cgaacatcaa cttgttcgaa tccggtga
//
LOCUS       DAOMUJ010000007        13104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1069_length_13104_cov_2.964825, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000007 DAOMUJ010000000
VERSION     DAOMUJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_13104_cov_2.964825"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PLT73_00475"
     CDS             complement(<1..1079)
                     /locus_tag="PLT73_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRM18887.1"
                     /translation="MKEEKHYGLFTAVSMIVGICVGSGIFFKADDILGATGGNVALGI
                     LVFCIGAFSIIFGSITLTQLANRTEKTGGVVAYFEEFISDKAASAFGWFQLFVYLPTI
                     TVVVSWVSGIYTCMLLGLPNTLETQTLIGTGYMAFFFLLNYFSLRLGGYFQNLATIMK
                     LIPLIAIAFVGIFWKEPAPIVPPELAVPITNVGWGWLAALAPMAFSYDGWTIATNITH
                     EVKNAKRTMPMALTIGPLLVLAIYLTYFSGLVSIAGPEYILAMGDEATTTVGISLLGE
                     RGGSIILSFVLIAILGVTNGVIMGILRLPQALAEKNMLPNSAKVKQIDPKSQLSPVAA
                     QIAFAIAFVWMVLHYLTQKSGIMGGG"
     gene            complement(1222..2094)
                     /locus_tag="PLT73_00480"
     CDS             complement(1222..2094)
                     /locus_tag="PLT73_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRM18888.1"
                     /translation="MNERYKDLKLAEQGARLSMVAYVTLSFAKLFIGNAFGSAALSAD
                     GLNNFTDVISTVAIMIGLRIARRPADEDHPYGHWKAETIASLATSLLMLLVGLQVLFS
                     SFSKLTSGEFTTPDVLSGITALIAGVIMIGVYIFNNRLANRINSLGLAASAKDNLSDA
                     LTSFATAIAIFGSIFGLYWLDGVMAVFVGIVIIKTAIDIFRESAFSLSDGFETNHLAE
                     YRQAILDIPDVQQVTSIAARSYGANVYVDVTILLDAELTVLRSHEITEAVEDCLRTVF
                     NVFETDVHVEPLTH"
     gene            2343..3797
                     /locus_tag="PLT73_00485"
     CDS             2343..3797
                     /locus_tag="PLT73_00485"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM18889.1"
                     /translation="MDRQKDALYYTELLRTKKVSPEELIVDAFKKIDRENPRYNAVVH
                     TRREKALHEARTRDFGNTLFGGVPILIKDLGQDLAGEPSTSGSQLLRNYVAPRTSHFI
                     EAIEKAGFIVIGQTSTPEFGFKNITDAVIHGPTRNPFNRDHSPGGSSGGAAAAVASDM
                     VAIATASDGGGSIRIPASFTGLVGLKPTRGRTPVGPGAYRGWQGASISFALTKTVRDT
                     AMMLDALQTIQAAAPFQTPLFPGSHFQEQQSGKHRKFRIAYSLESPVKSVVSADAKQA
                     VLQTVTVLEELGYEVEEKKPDIDGILLMEAYYAMNGAETDAMLRGFAAKLGRPLTADD
                     MELVSWSLYQYGQTISGGDYSLALGLWDQAAATMDQFHESYDLLLQPTTAAGAPRIDH
                     AFQSEELKERMRRADLLSAADRKEVVWTMFEESLGVTPFTQQANLTGQPAISLPVYRT
                     DSGLPLGVQFTAPKGKEEWLLDIAAVLERAGMFI"
     gene            4201..4590
                     /locus_tag="PLT73_00490"
     CDS             4201..4590
                     /locus_tag="PLT73_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM18890.1"
                     /translation="MKEKELRRSMAVFTIGTVMQLTDLTARQIRYYEEQELVHPKRTD
                     TNRRMFSLNDVDRLLEVKDYLAEGLNITAIKRMYSTERLKKQQEEELENKHKNLTDED
                     VRRILYKEILNASGFNPGDSNKPWGRL"
     gene            4630..5964
                     /gene="glnA"
                     /locus_tag="PLT73_00495"
     CDS             4630..5964
                     /gene="glnA"
                     /locus_tag="PLT73_00495"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRM18891.1"
                     /translation="MPKFTREEIIASAKNDNVRYLRLMFTDILGTIKNVEVPISQLEK
                     VLDNKMMFDGSSIEGFVRIEESDMYLYPDFNTWLVFPWGTEQGKVARLICDIYMPSGE
                     PFAGDPRNNLKRVLKEMKSLGFTDFNLGPEPEFFLFKLDEKGQPTLKLNDQGGYFDLA
                     PTDLGENCRRDIVLELEALGFEIEASHHEVAPGQHEIDWKYANAVEACDNIQTFKLIV
                     KTVARKHGLHATFMPKPVFGINGSGMHFNMSLFNENGNAFYDKEDERQLSATAYQFIA
                     GLIKHARGYTAVCNPIVNSYKRLVPGYEAPVYIAWSGKNRSPLIRIPESRGMSTRVEL
                     RSVDPAANPYLALAALLAAGLDGVKNKLEAPAPINRNIYQMSAEDRYENGIMDLPTSL
                     HEALGEFSKDQLLVEALGDHITNNFIESKEIEVMSYIQQVTEWEREQYLNMY"
     gene            6392..7282
                     /locus_tag="PLT73_00500"
     CDS             6392..7282
                     /locus_tag="PLT73_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM18892.1"
                     /translation="MEAIEMEGLTKRYRKSVALDNVHLTVEQGERFGFIGPNGAGKST
                     TIKLLLDFIKPSKGSARVFGLDAQKDSESLKRIIGYVPSDVHYYSNLTVEEILMMTGK
                     FHQIHNIKEMMAYYVERFALEPKKKMKDLSLGNRKKVAIVNSLIFNPELLILDEPTNG
                     LDPLMQHRLFEELNYHNKEGATVFISTHNLPEVEAFCTRTAFIREGKIISVEDMASGY
                     QPGKVVTLTGSAIVTKDFESKGYRVLSKTDRVVSLLYKDNVKQILADMAPLDLEDIEI
                     RNQNLEEKFMTLYGGGGSHE"
     gene            7275..8096
                     /locus_tag="PLT73_00505"
     CDS             7275..8096
                     /locus_tag="PLT73_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM18893.1"
                     /translation="MNSIRLEWKQERNGLFVWLFFMVLIMGLMMAMFPVMSNQGMEDI
                     LTTKLDAMPPEMLEAFSLTNGASLLQATGFFAYIFQYLFLAACIFATMKGAQALIKEE
                     TNGTIEYLYAQPISRKAIVTSKYIANLVLLLIFWIVLYGAALLFIYLFKEEGADFVEI
                     IKDITLMFGAESLVLLFFYSIGFLFSTFIASSRSTSGVALLLVFGTYMIGLVSKMVEE
                     YEFLRVLSPIEYAHPAALLEEGISWEYMVICIIGSLLALGLSYLIYGRKDLRIQV"
     gene            complement(8150..8323)
                     /locus_tag="PLT73_00510"
     CDS             complement(8150..8323)
                     /locus_tag="PLT73_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18894.1"
                     /translation="MNKYLTASILGIISIAINVWIMYQTRYDKGLNPITKKNLEKLSY
                     ALIVAAVLFMTFG"
     gene            complement(8355..8795)
                     /locus_tag="PLT73_00515"
     CDS             complement(8355..8795)
                     /locus_tag="PLT73_00515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM18895.1"
                     /translation="MWVIKPFEELTAGELHLIYKERTAVFVVEQNCPYQEVDDTDLVS
                     IHFWKQVDNRLLAYARLIPEESGVRIGRVLVPKSERTHGYGQELMQVVLEYAKTHFPG
                     LPIHAQAQAYLQAFYASFGFHPVSEIYLEDDIPHIDMIIATHRN"
     gene            complement(9269..10948)
                     /locus_tag="PLT73_00520"
     CDS             complement(9269..10948)
                     /locus_tag="PLT73_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM18896.1"
                     /translation="MFSSVDTLWTLLGTVLVFFMQAGFAMVETGFTRAKNAANIIMKN
                     LMDFVLGSLGFFLIGYSIMFGDDIAGIIGTPALFMEGLDSTIPGTVHFMFQNVFAATA
                     ATIVSGAVAERTKFSSYLVYSFIISLLIYPISGHWIWGGGWLAQMGFIDFAGSTAVHM
                     VGGVAALVGAAVVGPRIGKYKDGKAQVIPGHNLTIGALGVFILWFAWFGFNGASTVAA
                     TGDETLALIGTIFLNTNLSAVTATLVTLLFTWAKYGKPDVSMTLNGSLAGLVAITAGC
                     AVVSPFGAIAIGFIAGFAIVLAVEFVDQKLKIDDPVGAFSVHGVNGALGTILVGVFAT
                     DGGLLYGGGFNLLGIQTLGVTSVAAYTAGSMYVVFKVIQKTMGLRVTAEEEIVGMDVA
                     EHGLTSSYHDFVATTAFEDYLTDNSKPAAIPAIQEVDLSKVGAFKLADSGFTMNKVDI
                     IANPDKLERLKAELNAIGVTGMTVTSVHGYGLQKGQQTFYRGAKAEGNLLPKVRIETI
                     ISEVPVEEVIRAARRALYTGHVGDGKVFVSPVADAFRISNSDSGPAALKYY"
     gene            complement(11340..11624)
                     /locus_tag="PLT73_00525"
     CDS             complement(11340..11624)
                     /locus_tag="PLT73_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18897.1"
                     /translation="MRQCASWQNLFHLPSSYRYPTGSIQGYITRLNWTDEFIILEKEH
                     NEAFEYFIIDRRIESVKGPFSKEGFEKALKEEAIELPLIELSEMDWIINF"
     gene            12164..>13104
                     /gene="nrdD"
                     /locus_tag="PLT73_00530"
     CDS             12164..>13104
                     /gene="nrdD"
                     /locus_tag="PLT73_00530"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM18898.1"
                     /translation="MASLPTDYLNESTNLQVADTEVNIQVLKADGRVVPFHAKKIYKA
                     IKKAERFLSEQNEAAADHDVDAVVALVVDKLIASEAESVSTDDIHDQVEATLAEMGNL
                     PLAEAYNTYKLAKKVKKLQQQDIELRIQSLMNADQSVVNENANKDSRVFNTIRDLTAG
                     VAAKAIGLRMLPKHVSNAHMKGDIHYHDLDYQPYSPMTNCVLIDFQNMLREGFKIGNA
                     EVDSPRSVNTATAQMAQIFANVASSQYGGCSADRVDEVLAPYARLNYEKHLETAKEWV
                     DGEAKQIAYAEEKTRKDIYDAMQSLEYEINTLYTSNG"
BASE COUNT         3683 a         2912 c         3189 g         3320 t
ORIGIN      
        1 ccgcctccca taataccgga tttttgggtc agatagtgca acaccatcca cacaaaagca
       61 attgcaaaag caatctgtgc agcaaccggg gacagttgac tttttggatc aatctgtttc
      121 accttcgcgc tgttgggcaa catatttttc tcagcgagcg cctgcggcaa acgcaggata
      181 cccatgatta cgccattcgt tacacccaag atcgcgatca atacaaacga aagaatgatg
      241 ctgccgccgc gttcgcccaa caaggaaatg cccactgttg tagtggcttc atcccccatc
      301 gccaagatgt actcggggcc ggcgatggat accagtccgc tgaagtaggt caagtagatg
      361 gccaaaacca gcaagggtcc gatggtcaaa gccattggca tcgtccgttt ggcatttttc
      421 acttcatgcg tgatgtttgt cgcgatagtc cagccgtcgt aggagaaagc cataggtgcc
      481 aatgcagcaa gccaacccca accgacattc gtgatcggaa cagccaattc aggaggaacg
      541 atcggagcag gctctttcca gaaaatacct acaaaggcga tggcgatcag cggaatcagt
      601 ttcatgatgg tggcgagatt ctggaagtag ccacccaatc tgagcgagaa atagttcaac
      661 aagaagaaga acgccatata gcctgttccg atcagtgttt gtgtttccaa tgtgttcggc
      721 agacccaaca gcatgcaggt gtagatgccg gatacccagc tcacgacgac ggtgatcgtt
      781 ggcaggtaaa cgaacaattg gaaccagccg aacgcgcttg ctgctttatc ggagatgaac
      841 tcctcgaaat aagcgacgac gccgccggtt ttttcggtcc ggttagctag ttgcgtcaag
      901 gtgatgctcc cgaaaatgat gctgaatgca ccgatacaga aaaccaaaat ccctaatgct
      961 acgttcccac cggtcgctcc caaaatgtca tcagctttga aaaagatccc tgatccgacg
     1021 cagatgccga caatcatgga tactgcagta aataagccgt aatgtttctc ttctttcata
     1081 agtcactccc aatagatgta ttttttccca aaaaaaaaga cggacagatc tggatggcag
     1141 ctgtgtccgc caaaataatc tgtataaatc cgtctgctat tataccatca aaatgatgtc
     1201 aagcctatca aacttataac atcagtgcgt cagtggttct acatggacat ctgtttcaaa
     1261 aacattgaat accgtgcgca agcaatcttc gactgcttcc gtgatttcgt ggctgcgcaa
     1321 cactgtcagc tccgcatcca gcagaatggt cacatcaaca tagacatttg caccatagct
     1381 gcgtgcggcg atggacgtca cttgttggac atcaggaatg tcgaggatag cctgtcggta
     1441 ttcagccaaa tgatttgttt cgaaaccgtc ggaaagggag aaggcacttt cgcggaaaat
     1501 atcgattgca gtcttgatga tgacgatgcc tacgaagact gccatgacac cgtccaacca
     1561 gtacagcccg aaaatggagc caaaaatagc gatggccgtt gcaaagctgg taagggcatc
     1621 actcaggtta tctttcgcgg aagcggccaa gcccagactg ttgatgcggt tcgcgagccg
     1681 gttattgaat atgtagactc caatcatgat aactcctgcg atcaaggcag tgatacccga
     1741 cagcacatcg ggagtagtga actctccact ggtcagtttt gaaaagcttg agaacagaac
     1801 ctgtaggccg accagaagca taagcaagga agtggcgaga cttgcgatcg tctctgcctt
     1861 ccaatgacca taaggatgat cctcatcagc tggtcgacga gcgatacgca ggccaatcat
     1921 gatagcgacc gtggaaatga catcagtaaa attattcaat ccatcggcac tcagcgcagc
     1981 tgatccgaag gcattcccga tgaagagttt tgcgaaagag agggtaacat acgcaaccat
     2041 gctgagccgt gcgccctgtt ccgccaattt taagtcctta tatctttcgt tcatcgtgtc
     2101 ctactccatt tcctttgaat tcatccgggt gttcttttgc attttctgaa attgagacaa
     2161 tagaaggatt ctactatatt tcagtttggt tgtcatccaa aatgaatgga aatactgccc
     2221 ctcatcccat tgaaaaggac agaaaccggc acagcgacgc actggcccag gcaaaacatt
     2281 gcctttttct ggcgtgccac ctacaatgga aggaaatgcg aaaatgataa ggaggctttt
     2341 ttatggatcg ccagaaagat gccctgtatt acaccgaact tttaaggaca aaaaaggtat
     2401 cccctgaaga attgattgtg gatgccttca aaaagataga tagagagaat ccccgctaca
     2461 atgccgtcgt gcatacgcgc agggaaaaag cgctgcatga agccagaaca agggactttg
     2521 ggaacacttt attcggtggt gtgccgatct tgatcaaaga tttgggtcag gacttggccg
     2581 gggagccgtc aacatcaggc tcgcagctgc tgaggaatta tgtcgctccc cgcaccagtc
     2641 attttatcga ggcaattgaa aaggccggat tcatcgtcat cggccagacg agtacgcctg
     2701 aattcggatt caaaaacatt actgatgccg tcatccatgg accaaccaga aaccccttta
     2761 atcgggacca ttctccggga ggttccagcg gcggtgcggc agctgccgtg gcatcagata
     2821 tggttgccat cgccaccgcc agtgacggtg gcggttcgat ccggattccg gcctctttca
     2881 caggtttggt ggggctgaaa ccgacaagag ggcggacgcc ggtggggcca ggagcatatc
     2941 gcggctggca aggcgcatcg atcagcttcg cattgacgaa gacggtaagg gatacagcga
     3001 tgatgctcga tgctctgcag acaatccagg cggcagcgcc gttccaaacg cccttgtttc
     3061 caggcagcca tttccaggaa cagcaatccg gaaaacatcg caagttccgg attgcctatt
     3121 cactcgaatc accggtgaaa tctgtcgtca gtgcggacgc gaagcaggct gtactccaga
     3181 cagtgacagt attggaagag ttggggtacg aagtggaaga aaaaaaacct gatatcgacg
     3241 ggatcctttt gatggaggcc tactatgcaa tgaatggagc cgaaacggat gcgatgctgc
     3301 gagggtttgc cgcgaaactg gggcggcctt tgacggccga tgacatggag ttggtttcct
     3361 ggtctctcta tcaatacggc caaacaatct ccggtggcga ctactccttg gcattgggcc
     3421 tatgggacca ggctgcggcc acaatggatc agtttcatga aagctatgac ttgctgctgc
     3481 agccgactac agcagcaggt gctccccgca ttgatcatgc tttccaaagc gaggaactga
     3541 aggagcggat gcgcagagcg gatctgttgt cggcagcgga ccggaaagaa gttgtctgga
     3601 ctatgtttga agagagtttg ggcgtgaccc cattcacgca gcaagcaaat ttgaccgggc
     3661 agcctgccat cagtctgccg gtataccgaa cagattccgg cttgcctttg ggggtgcagt
     3721 tcactgctcc taaagggaaa gaggagtggc ttttggatat tgcggcggta ctggagaggg
     3781 caggtatgtt catctaagtg aaggcgctga taattgcggc aatccaaacc ctctgcaggt
     3841 gcttatcgtc gcagccgttt cgttactcac attttttttt aatatttttt tcattaaagg
     3901 gctctttttt ttatcccatg cgagggaatt ggcgtgtgag ggggagatga aatgcctttc
     3961 aaatgttatt ctgacaaggt tttacgtaga tgttagaaaa tgtgacatag gatgtaaagg
     4021 tagcgttgac ttgaatgaga attaggagta ttcttgttgt attaaatgac tcaattaatt
     4081 gctagtaaat acgattaatt gctgaaaaga actatctttt tctcaacagg cactaactcc
     4141 tgaagaaaaa ggatacatct gtgtttcgct caactcagaa gcttcgatgg aggggttggt
     4201 atgaaagaaa aagagctacg acgttcgatg gctgttttta ccatcggaac cgtcatgcaa
     4261 ctgacggatc tgacagctag acagatccgc tattacgaag agcaagaact cgttcatcct
     4321 aagcgcacag atactaatcg ccggatgttt tcattgaacg acgtcgatcg tctgctggaa
     4381 gtcaaagact atttggctga aggattgaac atcactgcaa tcaagcggat gtacagtacc
     4441 gaaagactga agaagcagca agaagaggag cttgaaaaca agcacaaaaa cttgactgat
     4501 gaagacgtcc gtcgcatcct ctacaaagag atactcaacg caagtggatt taaccctggg
     4561 gattcaaaca aaccttgggg aagattataa aaaaaacaaa caaagcacag tcaaagagag
     4621 ggaatacaaa tgccaaaatt tactagagaa gaaatcatcg catcagcaaa gaatgataac
     4681 gtccgttacc tacgcttaat gttcactgac attttaggaa caatcaaaaa cgtagaagtt
     4741 cctatcagcc agttggaaaa agtattagac aacaaaatga tgttcgacgg ttcttccatc
     4801 gaaggttttg tacgtattga agaaagtgat atgtacttgt acccagattt taatacatgg
     4861 ttggtattcc catggggaac tgaacaaggt aaagttgcac gcctgatctg tgatatctat
     4921 atgccaagcg gcgaaccatt tgctggagat ccacgcaaca acttgaaacg cgttctaaaa
     4981 gaaatgaaaa gcttgggctt cactgatttc aacttaggac ctgaacctga attcttcctg
     5041 ttcaaattgg atgaaaaagg tcaacctaca ttgaaattga acgatcaagg cggatacttc
     5101 gacttggctc ctactgactt gggcgaaaac tgccgtcgtg atatcgtatt ggaattggaa
     5161 gctttgggct ttgaaattga agcaagccac cacgaagttg ctccaggaca gcacgaaatc
     5221 gactggaaat acgcaaatgc tgttgaagcc tgcgacaaca tccaaacatt caaattgatc
     5281 gttaagaccg tagcccgcaa acatggcttg catgcaacat tcatgcctaa gccagtattc
     5341 ggcatcaatg gttctgggat gcatttcaat atgtcattgt tcaacgaaaa cggcaatgct
     5401 ttctacgaca aagaagatga acgtcaatta agcgcaacag cttatcaatt catcgctggt
     5461 ctgatcaaac atgcgcgcgg atacacagct gtctgcaacc caatcgttaa ctcgtacaaa
     5521 cgtttggtac ctggctacga agcaccagta tacatcgcat ggagcgggaa aaaccgttca
     5581 ccgctgatcc gtatcccaga atcacgtggc atgtccactc gtgttgaatt gcgtagtgtt
     5641 gacccagctg ccaacccata cttggcttta gctgcattat tggctgccgg tcttgatggc
     5701 gtgaagaaca aattggaagc tccagctcca atcaaccgca acatctacca aatgtcagct
     5761 gaagaccgtt acgaaaacgg aattatggac ttgcctacat cgttgcatga agctcttgga
     5821 gagttctcta aagatcaatt gttggttgaa gcattaggcg accacatcac caacaacttc
     5881 atcgaatcaa aagaaatcga agtaatgtct tacatccaac aagtaacaga gtgggaaaga
     5941 gaacaatact tgaatatgta ctaagagagt gcgatgaatc ccgtttagga atcgagtact
     6001 aagaggatag ccacaacgcg acccggtttt ggtcgcgatg tggctatcag cttagacgga
     6061 gatttctggg attcagaaca cgtttagaga gagtgcgatg atttgcggga ggggatgagc
     6121 actaagagga ctaacactaa acggacgttt tttgtccgtg tgtggtagtc agcttaggcg
     6181 gaatcccctg caaatcagaa cacgtttaaa gagcgggatt cagaacgcgt ttaccgagag
     6241 tgtaagaatc ccgctcttct acaaaaggcc gagaccaatt tgtctcggcc ttttgtgtcg
     6301 ttcagggaag aaaaatcttg ctgttgctgt aaacttcagc cgagaaaacg tataatggat
     6361 atatgtaaag ttctttagag gaggggttac agtggaagca atcgaaatgg aaggtttgac
     6421 caaacgatac cgtaagagtg tggctttgga caatgtccac ctcacagtgg agcaaggcga
     6481 acggtttggg ttcatcgggc cgaacggagc agggaagtcg acaaccatca agttgttgct
     6541 tgatttcatc aagcccagca aaggaagtgc ccgagttttc ggactggatg cccagaaaga
     6601 ctccgagagc ctgaagcgca tcatcggata tgtcccgagt gacgtgcact actacagcaa
     6661 tcttaccgtt gaagagattt tgatgatgac ggggaaattc caccaaatcc ataatattaa
     6721 ggaaatgatg gcttattacg tggaacgctt cgcattggag ccgaaaaaga aaatgaagga
     6781 cctttccttg ggtaatcgga aaaaagtcgc gattgtgaac agtctgattt tcaatccgga
     6841 acttttgata ctggatgagc ccacaaacgg actggatcct ttgatgcagc atcgtttgtt
     6901 tgaggagctg aactaccaca acaaagaagg cgcgacagtc ttcatttcta cgcataatct
     6961 gccagaagtg gaagcattct gtacgcggac tgccttcatc cgtgaaggca aaatcatttc
     7021 cgtggaagat atggcttccg gttatcaacc agggaaagtg gtgacgctga ccggttcagc
     7081 catcgtaacg aaggatttcg aatccaaagg gtatcgcgtc ctttccaaga cggatcgggt
     7141 cgtttccttg ctctacaaag acaacgtgaa gcagattctg gccgatatgg cgccgctgga
     7201 tttggaggat atcgaaatcc gcaatcagaa tctggaggaa aaattcatga ccctgtatgg
     7261 tggaggtgga tcccatgaat agcatccgtt tggagtggaa acaggagcgc aacggtttgt
     7321 tcgtgtggtt gttttttatg gtcctgatca tgggcttgat gatggcgatg ttccccgtca
     7381 tgtcgaatca aggaatggag gacatcctga cgaccaagct cgatgcgatg cctcctgaga
     7441 tgctggaggc tttctcgctc acgaatggcg cgagcttgct gcaggccact ggattttttg
     7501 cctacatttt tcagtacctc tttttggcgg cctgcatttt tgcaacgatg aagggtgcgc
     7561 aggcgctgat caaggaagag acgaacggta caatcgagta tctttatgca cagccaatca
     7621 gcagaaaagc tattgtcacg agcaaataca tcgctaactt ggtattactg ctgatttttt
     7681 ggatcgtttt atatggcgca gctctgctgt tcatctatct attcaaggag gaaggagccg
     7741 attttgtgga aatcataaag gatatcacgc tgatgttcgg tgcggagtcg ttggtattgt
     7801 tgttctttta ttcgatcggc ttccttttct cgacgttcat tgcgagtagc cgttcaacaa
     7861 gcggagtcgc gctattgttg gtgttcggca cctacatgat cggccttgtc tcgaaaatgg
     7921 tcgaggaata cgaatttctg cgggttttgt cgccgatcga atatgcacat ccagcggcct
     7981 tgttggaaga ggggatttcc tgggagtata tggttatttg catcatcgga agcttactcg
     8041 cgttagggtt gagctatctg atttatggca gaaaagatct gcggatacag gtgtagtatt
     8101 gtctatcaac aggaataaac aaaaaaagcc cattgcgggc tttttttgtt taaccaaatg
     8161 tcatgaaaag tacagcggcg acgatcagcg cgtaggataa tttctccaga tttttctttg
     8221 tgatcgggtt caggcctttg tcgtagcgtg tctgatacat gatccaaacg ttgattgcga
     8281 tgctgatgat tccgagtatg cttgcggtaa gatatttatt cacgtttttc ctcctgttgg
     8341 tttatgtttt ctgtttagtt acgatgcgta gcgatgatca tgtcgatgtg cgggatatca
     8401 tcttccagat agatttccga tacgggatgg aatccaaaag aagcataaaa agcctgcaga
     8461 taggcttgtg cttgcgcatg gatcgggagt ccggggaaat gcgtttttgc atattccagc
     8521 acgacttgca tcaattcttg gccgtagcca tgggtgcgtt cgcttttcgg aaccagaaca
     8581 cgtccgatgc gcactcctga ctcttccgga atcaaccggg cgtaggccag taatcggttg
     8641 tccacctgct tccagaagtg gatgctgacc aggtcggtgt catcaacttc ttgataagga
     8701 caattttgct ccacaacgaa aaccgctgtc cgttctttgt agatcagatg cagttctccc
     8761 gccgtcagtt cttcaaaagg tttgatgacc cacatatatt ttcctcctca ttgcgtggta
     8821 aaaaaatacc ttcacagtcc attatagcaa agcggggaga aacaaaaaac cggtaatctt
     8881 ttgctcaaaa aatgaagcca aggagttttc tcatagagag gactccttgg cttcatcaat
     8941 gccattaaat taagggtgtt gacaaataag tggtagagga actatgagag aaaacctctc
     9001 atttatccgt cttggatcag aattcataag aaatgctcag cgcaggggca aaattgtcga
     9061 atagatgcgg tagatccgac aattttgaat cagtttgagc cgatattgtc gaatgtcgag
     9121 aaactttcga caatttgaca gcctgtcgaa ctgatattgt cgaatagctg aggaacattc
     9181 aacaaattgt tcccgctcgc ttttttcgga agtccagctg cgtaagttaa tgacgttgtt
     9241 ggcttcatta agtcttttgt tattcggttt aataatattt aagggctgct gggcctgaat
     9301 cgctgttgct gatacggaaa gcatcggcaa ccggggaaac gaagactttg ccgtcaccga
     9361 catggccggt gtacaaagca cggcgggcag cacggatgac ttcttcgaca ggtacctcgc
     9421 tgatgatcgt ttcgatgcgg actttcggca acagattgcc ctcagctttc gcaccacgat
     9481 agaaggtttg ttgacctttt tggagcccgt aaccatgtac ggatgtgacc gtcatgccgg
     9541 ttaccccgat agcattcaat tccgctttca gtcgctccag cttatcagga ttggcaatga
     9601 tgtccacttt attcatcgtg aaacctgaat cagccaattt gaaggcaccg actttgctga
     9661 gatccacttc ttggatagca gggattgctg caggtttgct gttgtcagtc agataatctt
     9721 cgaaagcagt cgttgcgaca aagtcatggt agcttgaagt caaaccatgc tcagcgacgt
     9781 ccataccgac gatttcttct tcagcagtaa cgcggagacc cattgttttt tggatgactt
     9841 tgaagactac gtacatggaa ccagctgtgt aggcagctac agaagttaca cccaacgttt
     9901 gtatgcccag caggttgaat ccaccgccgt acaataatcc gccatctgtt gcgaaaacac
     9961 cgactaagat tgttcctaag gcaccgttca cgccgtggac actgaaagca ccgacgggat
    10021 cgtcgatttt caatttttga tcgacgaatt caacggcaag gacaatggca aagccggcga
    10081 tgaatccgat ggcgatggcg ccgaaaggag agacgaccgc acaaccggca gtaatggcta
    10141 cgagacctgc caatgaaccg ttcagcgtca tcgaaacatc gggtttgccg tatttcgccc
    10201 aagtgaataa gagcgtgacc agagtcgctg tgactgcgga taagtttgta ttcaagaaga
    10261 tcgtaccaat caaggcaagg gtttcgtctc cagttgccgc aaccgttgat gctccgttga
    10321 atccgaacca tgcgaaccag aggatgaaga cgccaagggc accgatcgtc aaattgtggc
    10381 caggaatgac ttgtgcttta ccatctttat attttccgat acgcggaccg acgacggcag
    10441 ccccgacaag agcagcaacg ccacctacca tatgcacggc tgtggatcca gcgaagtcaa
    10501 tgaagcccat ttgagccagc caaccgccgc cccagatcca atgtccggag atagggtaga
    10561 tcaacaagga aatgatgaaa gagtacacga gataagagct gaattttgtc cgttccgcaa
    10621 cagctccgga tacgatagtg gctgcagttg ccgcgaatac gttttggaac atgaaatgga
    10681 cagttccagg aatggtggaa tcaagtcctt ccataaataa agcaggagtt ccgatgatgc
    10741 cggcgatatc gtccccaaac atgatgctgt agccgataag gaagaatccc aatgatccga
    10801 gtacaaagtc cataagattt ttcatgatga tgttcgctgc gttcttggcg cgagtgaatc
    10861 ccgtctcgac catcgcgaaa ccggcttgca taaagaatac caataccgtt cccagcaagg
    10921 tccataatgt gtctactgat gaaaacataa atgattcctc cttttgtttt agcatagtct
    10981 tagtgtatag aaataatttt attgatgcta tatcctgctt acgcaccatg taatataatg
    11041 tgacaatgct tacaaatcaa tgtttgcaag cgcttggatg cgaaaatgat gagatttctg
    11101 agcttaaatc tcatcaaatt gcagggatgt tggatgcggt tgtgtttttt tggtcaacat
    11161 gattaaaata aatctgacat gagcgagtag ggaataggac ggatgtcaga atcggtgaca
    11221 acgaataggt tccatcagca gggatatccg gggtaaagag caaatataca gcaaaaagcc
    11281 gtaagggaaa catgcgggac atgttttcct tacggctcta tgtattcttt tagtaacttc
    11341 taaaagttga tgatccagtc catttccgat aattcaatca atggcaactc gatcgcttcc
    11401 tcttttaacg ccttctcgaa cccttccttc gaaaaagggc cttttacaga ttcaattctt
    11461 ctatcgataa taaaatattc gaatgcttca ttatgttctt tttcaagaat gataaattca
    11521 tcggtccaat tcaatcgtgt aatatagcct tggatgcttc cagtcgggta gcgataagat
    11581 gaaggcagat ggaaaaggtt ttgccaagaa gcgcactgtc tcaaaaatag gccttctccg
    11641 accggtattc taaatttagc aattctggat atttcaggca acattctatt tttccacact
    11701 atcatttcgc ccctttttat ctcctcattc ttttgacatc cctgtggctt aaagccggat
    11761 aacagatccg aaaataagaa tgaatcgctt acatttctga caagtcatag tataactgct
    11821 tttttttaca aagtacagta gattctattg attttatgta ctttatatgt tttttaacat
    11881 ttttttaatt ttcagggtat tttcctattg accaaagcat caaaatatgg ctgccgaatt
    11941 tagacggaat ttcgctttaa acactatata ttgtggtgga aatttctctt tggatgatat
    12001 atagtataca aagagaatcg aaacagagtc tactttaagt agaggcaacc aacgtgagaa
    12061 gcgcgtccga tagggaaagc ttgctcactt tgtaacgacc tcttactttt tattagcttt
    12121 ttttgcatgt tcggttgatt tttaactaag gagtgggaaa aatatggctt cattaccaac
    12181 agattactta aatgagagca cgaatcttca agtcgctgac acagaagtga acatccaagt
    12241 attgaaggcg gatggacggg ttgttccttt tcatgccaag aaaatatata aagcaatcaa
    12301 aaaagcggag cgttttttat ctgaacagaa cgaagcggct gcagatcacg atgtggatgc
    12361 tgtcgtggca ctggttgtag acaaactgat tgcgtcagaa gcggaaagtg tttcgacgga
    12421 tgatatccat gatcaggttg aagcgacgct tgctgaaatg ggcaatctgc cattagccga
    12481 agcctacaat acctataaat tagcgaagaa agtcaaaaaa cttcaacaac aggacatcga
    12541 attgcgcatc cagagcctga tgaatgccga tcagagcgtc gtcaacgaga atgccaacaa
    12601 ggacagccgt gtcttcaata cgatccggga cttgacggca ggagtggccg cgaaagcgat
    12661 cggcttgagg atgttgccga aacacgtttc caacgcccac atgaaaggcg atatccatta
    12721 ccatgacttg gattaccaac catattcacc gatgacgaac tgtgtgctga tcgatttcca
    12781 gaatatgctc cgtgaaggtt tcaaaatcgg taatgccgaa gtggacagcc cgcgttcagt
    12841 caacacggcg actgcccaaa tggcgcagat ttttgcgaac gtcgcttcca gtcaatacgg
    12901 cggctgcagc gcagaccggg tcgacgaagt gttggcacct tacgcacgcc tgaactatga
    12961 gaagcacctt gaaacagcga aagaatgggt tgatggcgaa gcgaaacaga tcgcttatgc
    13021 cgaagaaaaa acccgcaaag acatctatga tgctatgcaa agcctggagt acgaaatcaa
    13081 cacactctac acttcaaacg gaca
//
LOCUS       DAOMUJ010000008        12496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1145_length_12496_cov_2.662407, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000008 DAOMUJ010000000
VERSION     DAOMUJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 12496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12496
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_12496_cov_2.662407"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1913
                     /locus_tag="PLT73_00535"
     CDS             <1..1913
                     /locus_tag="PLT73_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM18899.1"
                     /translation="GKVLHEELSLEEGAVIFWEKRYRDYQVQALVAACDHYGMPLKPN
                     TPVRYFTPAQRLLLLQGAESETVRRLFPDVPVPRTVAEGRYEGVLPNLWRRMADQKGT
                     SKLREAYFESAICPECHGERLNELSRSVTVKGTRLPELSGSALKELVDWLSGLAANLA
                     HSEQLLAGVYVTDLKTKLKRILKLGLGYLSLDRQTMTLSGGEAQRLRLAAVLDSDLTG
                     VIYILDEPTISLHPKDTAGLLTVLRSLRDKGNTVLVIEHDTAVMAAADYLIDIGPGAG
                     KYGGEVIGSGTLQQIKDQAASVTGAYLNSNPEPREIFHKGSGTFIEVSQANKHNLRNL
                     TVRFPIGCLICVTGVSGSGKSTLVFDVLAKGDTAPSEDPNRVSGTGSFDRIVVVEQAP
                     LTRMKRSNVATYSDAYTEIRKIFSRQQTSIEKGMTANHFSFNVKGGRCDHCEGLGVVT
                     NNLLFFEDQEVPCPVCSGRRFKDEVLSVTYRGHSITGVLGLSVEEGAELFADSPKISA
                     ILELLQEVGLGYLELGQTTTTLSGGEAQRLKLAVELLVNQGKKNLYLIDEPTTGLHPL
                     DVEKFLKLLDRMVGSGSTVVVVEHNQQIIGAADWIIDLGPGGGDAGGTIIAEGTPSEI
                     RKNENSVTGMFI"
     gene            2253..>2400
                     /locus_tag="PLT73_00540"
     CDS             2253..>2400
                     /locus_tag="PLT73_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM18900.1"
                     /translation="MNLQQLRYVITVAKYGTFREAARQLYMAQSSLSSSIKDLEEEYG
                     IQIFE"
     assembly_gap    2401..2410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2411..3193
                     /locus_tag="PLT73_00545"
     CDS             <2411..3193
                     /locus_tag="PLT73_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM18901.1"
                     /translation="SKKGIEITKEGAMLLEFADQIVAQADQLDYRYLSATENRRLFSV
                     SSQHYDFASEGFARLVSEKKEESLNFRFLETSTSKVMEDVRDAVSEVGFIYLNDFNGK
                     VIKQYLEAFDLKFDPLIAFQPHVFLSESHPLAKQAKISQEDLHPYPVISFDQSKNSTL
                     QLMEESIQVDQNAQRISASDRATVLNLLSVTNGYLVGSGLLKSNTQDHIVVVPMDVDQ
                     KNTIGFIYSKVRPLSKISKRYMEIVRELLEGDQRIELTDHVD"
     gene            complement(3329..4435)
                     /locus_tag="PLT73_00550"
     CDS             complement(3329..4435)
                     /locus_tag="PLT73_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18902.1"
                     /translation="MARKMGKLKFALIGIVIAGILTLVLFTKNGKIERLTFAEETNTE
                     ASLGSLDELMERLSDYPELYRAAFEEDGINPEDNIFIIPGMDATLTLHHEHEEADICT
                     TMTPQGLAVNKDYLFISAYCQTKEHNSVIYVLEKKSGSFIKEIVLDGIPHAGGLAYDN
                     NHELLWVTSEKGDTAQVITLSLKDIEAYDFADSAEPITYTDSIELPDIKRASTITVHG
                     DDLYVAHFDDEETSVMHRYLISTTEDEDGKEVHALDTGKSTEEAYPDDVDKIGKEIQG
                     LAFYREKLMLSRSAGRKKDSTLLSFDRLKETENFTDKNASTEITMPSYLEQIAVDGKQ
                     LYILFESGAYPYRAHGNPSIDRVLRVEIDSLFAE"
     gene            4755..5255
                     /locus_tag="PLT73_00555"
     CDS             4755..5255
                     /locus_tag="PLT73_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18903.1"
                     /translation="MSKLGLQLSPADSESKCWVAEITGADEVYILKRDFIPAEPEGGW
                     ILYDGWYQLNGAVPGVTEFKKEYIRIKDGKVRRNLPFRELVESLDEIKAGEGPRVERM
                     RKEIIAILDEIKEAAYCEPVVEGIEKQKEDLDMADEPDQIKNALYMLKKQKQSYIQQY
                     RKMFNL"
     gene            5356..5856
                     /locus_tag="PLT73_00560"
     CDS             5356..5856
                     /locus_tag="PLT73_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM18904.1"
                     /translation="MIRKAMPADLPAIMEIINEAKVTLKASGIDQWQKGYPNEAVILQ
                     DMAGGYSYIYLRDGIPAATFAFFYGEDPTYEVITEGQWLTDNPYTVIHRITIKGTFRG
                     QGVLGEIVAWAGDESRKRGYSSMRIDTHPDNKSMQRALQKAGYTYCGHILTSIGDMRW
                     GYEKLL"
     gene            5959..6492
                     /locus_tag="PLT73_00565"
     CDS             5959..6492
                     /locus_tag="PLT73_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM18905.1"
                     /translation="MNVQLKNLPLNEELIQAVRRWENDPAISPFITPHYLETSELPLV
                     TESDVRESLSNPDKRNYFICVDDKPIGIASIIRDFPALLKNDGKTAWLGIYIGEPDWR
                     NKGIGKTVMALYEEKCRSLGYRRIELGVFSHNAGAIRLYERSGFTQIGVIPHFTYSQG
                     QWRDDIRMEKILCHNLG"
     gene            6533..7654
                     /locus_tag="PLT73_00570"
     CDS             6533..7654
                     /locus_tag="PLT73_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM18906.1"
                     /translation="MKKGVLVRSKRILLTFFAALLGLLTLFSYHNYAWYEEPIAKITA
                     AEAVGTDETIGAGQENYPIYNQKLTAVLMNGESKGTQVVIENNYSDSLAYSQMYRSGD
                     EVFLSLSERASGTLTGKISGIKRDKYVVLFGSLFLFVLVAVAGKKGLLSLVSIAGNIL
                     LFNIALDSYEIADNAQLLWISSAAVIGWTVLTLLLVSGRNRKTYAAILSTLIGTFLTL
                     IIAYGVIAGTNGQGLRYEEMQFVTHYPRRIFLSSILIGSLGAVMDVAITMTAAIQELY
                     EKDPAISAKALFQSGREIGKDIMGTMANVLLFAYVSGAIPTLVFYLENGSDVFHTFSM
                     QLSLEITRALAGSLGIVLTVPLAMLTAVRLIPKKAGDRR"
     gene            7651..8412
                     /locus_tag="PLT73_00575"
     CDS             7651..8412
                     /locus_tag="PLT73_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM18907.1"
                     /translation="MSVQLVLALLLLLLMVVVGGRRGISSFLSLFFNFAVLYVLVLLL
                     INGFPALVVTLLACAVVSYINLFYINGVNVKTKAAFIATIVTTFFLLLLTIVVHRFAM
                     IQGFGPEEVEEYTTLSFFVNVNFVQIGICTMITGTIAAITDTAISISSSLYEVHNCNP
                     QLGEKELFRSGMTIGKDIIGTTTNTLYFSFVGSYLALFLWFRDLSYSFGEALNAKVLV
                     SEVLSMFVIGIGVTGIIPLTAWVTAHMLLNESSRD"
     gene            8566..10074
                     /locus_tag="PLT73_00580"
     CDS             8566..10074
                     /locus_tag="PLT73_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HRM18908.1"
                     /translation="MKKIGFDPQKYIEEQSKYILERVNNYDKLYLEFGGKLIGDKHAK
                     RVLPGFDEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDVFRLIDELR
                     DYGLSVNSVVITRYNGQPATKVFINKLEKRDIKVYTHGAIEGYPSNIEKIVSEEGFGQ
                     NTYIPTTKPIVVVTAPGPGSGKLATCLNQLYHERRQGRVAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMMDNYHFETYNEVAVNYNRDLETFPVIKRIIERITGKESVYQ
                     SPTDMGVNRVGFGITDDEAIREASKQEIIRRSFDTECDYKKGLSDEETRDHMRLIMEE
                     LELKQEDRAPVKPARAYAERLMKRTVDNEIPAVIAFELNDGRIITGRTSDLMDACSSA
                     ILNAIKALANISDDILLLSPVILEPIKKLKSDGLHKRIPTLTANEILIALSISAVTNP
                     TAQLAYDKLSELKDVQAHSTVILNKDDDQILRNLGLDITCDPVYPSENLYYV"
     gene            complement(10293..10733)
                     /locus_tag="PLT73_00585"
     CDS             complement(10293..10733)
                     /locus_tag="PLT73_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003639274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM18909.1"
                     /translation="MDKIDKQILNILQTNARASLKEIGEATFLSSPTISARIAQLERN
                     GTITDYGAHVNLQALGYNIKAFINLEVDPKQKPVFYPFVEGIPNVLECNCVTGEFSML
                     LKVAFPTTKELDTFIGELQQFGKTYTQIVFSTSVGPRGIHFEDQ"
     gene            10995..12410
                     /locus_tag="PLT73_00590"
     CDS             10995..12410
                     /locus_tag="PLT73_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRM18910.1"
                     /translation="MNMRIEADSIGTLAVPTEAYYGVQSSRAKENFAITGTNLHPELV
                     QSLAWIKKAAAVVNAQSGNLTAVKKDAIVQACDEIMAGYFHEAFIVDAIQGGAGTSAN
                     MNANEVIANRAIEILGGRKGDYRIVHPNDDVNLCQSTNDVFPTAGKMTVLQILPELIA
                     ELEKLESALGEKAEAFAGVIKMGRTQMQDAVPTTLGRTFRAYQSFVKRDIRHLMSAAE
                     EMKTLNLGGTAIGNTINATPEYLENITAFVSEEVGIAFTQADDLFDATQNVDGFVRVS
                     AAIKTAAVNLSKMSNDLRLLSSGPKAGIGEINLPAKQNGSSIMPGKVNPVIPEVVSQV
                     AFRVIGNDLTITMAAESGQLELNAFEPIIFHSLFESIDCLTHAVATLTANCITGIQAN
                     EMICYKQVEESAGIATALCPTIGYQKASAIAKESLETGKTVRELVLEKGLLTIAETDS
                     ILDLKHMAGIPAAILLRDKVI"
BASE COUNT         3328 a         2872 c         3263 g         3023 t
ORIGIN      
        1 aagggaaagt gctgcatgaa gagctgtcgc ttgaagaggg tgcggtcata ttttgggaaa
       61 aacgctaccg ggattaccag gtgcaggcgc tggttgccgc gtgtgaccat tacggcatgc
      121 cgctgaaacc aaatacccct gttcgatatt ttactccggc gcagcgctta ctgttgctgc
      181 aaggtgctga aagtgaaacg gtgcgtcggt tattcccgga tgtgccggtg ccccgaacgg
      241 ttgctgaagg ccggtatgaa ggtgtgttgc cgaacctttg gcgcagaatg gcggatcaaa
      301 aagggacttc caaactccgg gaagcctact ttgaatctgc tatctgcccg gaatgccatg
      361 gcgagcgatt gaatgagctg agcaggagcg tgaccgtgaa ggggacgcgc ttgccggaac
      421 tgtccgggtc agcattgaaa gagcttgtgg attggctcag cggacttgct gcaaacctgg
      481 cgcattcgga gcaactgttg gcgggtgtct atgtcacgga tctgaagacc aaactgaaac
      541 ggatcctgaa gttgggattg ggctatctgt cgctcgacag gcagacgatg acgctgtcgg
      601 gaggggaagc gcagcgtctg cggttggcgg ctgtcctcga ttcggatctg acgggtgtca
      661 tctacatcct ggatgaaccg acaatcagtc tgcatccaaa agatacggcc gggctcctga
      721 cggtcctgcg gtcgctgagg gacaaaggca atacggtgct cgtcatcgaa catgacacag
      781 ccgtcatggc ggcagcggat tacctgattg atatcggtcc gggagccggg aaatatgggg
      841 gcgaagtcat cggatcggga acgctgcagc aaatcaagga tcaagcggca tccgtcaccg
      901 gtgcctatct gaacagcaac ccggaaccgc gcgaaatttt ccacaaagga tccggtacgt
      961 tcatcgaggt gagccaagcg aacaagcata acctccggaa cctgacagtc cgtttcccta
     1021 tcggctgtct catctgtgtc accggcgtat cggggtccgg taaatcgacg ctcgtgttcg
     1081 atgtgctggc gaaaggggac actgcccctt cagaggatcc gaatcgggtg agcgggaccg
     1141 gctcattcga ccggattgtc gtcgtggaac aggccccgct cacccggatg aagcgttcga
     1201 atgtcgcgac ctattcggat gcttatacgg aaatccggaa aatcttcagc cggcagcaga
     1261 cttccatcga aaaaggaatg accgccaatc acttttcctt caatgtgaaa gggggacgct
     1321 gcgatcattg cgaaggcctg ggcgttgtta caaataacct gctgtttttt gaagaccagg
     1381 aagtcccttg tcctgtctgt tcgggcagac gcttcaagga tgaagtgctg tccgtgacct
     1441 accgcggcca ttcgatcaca ggagtgctcg gcttgtcggt ggaggaaggc gcggaactgt
     1501 ttgcggattc gcccaaaatc agtgccatcc ttgaactgct gcaggaggtt ggtctcggct
     1561 acctcgaact aggccagacg acaacgactt tatccggcgg cgaagcgcaa aggcttaagt
     1621 tggcagtgga actcctcgta aatcagggaa aaaagaatct ctatctgatc gatgaaccga
     1681 ctaccggtct gcatccgctc gatgtcgaaa aattccttaa attattggat cggatggtcg
     1741 gttccggcag cacagtggtc gttgtggaac acaatcaaca gatcatcgga gcagctgatt
     1801 ggatcatcga ccttggtccg ggtggcgggg atgccggtgg aacaatcatt gcggagggaa
     1861 cgccatcaga aatccggaaa aacgaaaatt cggtgacagg aatgttcatt taaggctgac
     1921 caagtgaaaa tgattgacag aaaccggaaa aatcgctatc ttattagaaa gcgattagaa
     1981 ggatagggga ggcaaaacca tgcagaagat gatgtgttgt atgtgcggat gtaagaaaca
     2041 tgcaggaata ttcttatcat cttttggtct ccacaaccgg gtgtttttgc cggctttgta
     2101 aaggggatca ggggcagatt gtgcatccac atccaagtga agcgctgaaa tgatgatgaa
     2161 tactcgtgca tgtgtgtttt gttgacgcgc atgcactttt tttgtggaaa cgatgggacc
     2221 ccatcggaaa atagagccaa ggaggcaaaa atatgaacct gcaacaattg cgttatgtca
     2281 tcacagtagc gaaatacggc acttttcggg aggccgcaag gcaattatat atggcgcagt
     2341 ccagtctgtc gtcaagcatc aaagatctgg aggaagaata tggcatccag atcttcgagc
     2401 nnnnnnnnnn tccaaaaaag ggatcgaaat aaccaaagaa ggggctatgc ttttggaatt
     2461 cgccgatcag atcgtagccc aagcggacca attggattac cgctatctgt ctgcgaccga
     2521 aaacaggaga cttttttccg tctccagcca gcactatgat tttgcttcgg aaggctttgc
     2581 ccgactggtt tcagaaaaga aagaggaatc attgaatttc cgtttcttgg agacgagtac
     2641 ttcgaaagtg atggaagatg tgcgcgatgc agtcagcgaa gtcggcttca tctatctgaa
     2701 tgatttcaac ggaaaagtca tcaagcagta tctcgaagcg ttcgatctga agttcgatcc
     2761 gctgattgcc ttccagccgc atgtatttct cagcgaatcc catccgttgg cgaagcaagc
     2821 gaagatatct caggaggatc ttcatcccta tcctgtcatt tccttcgatc aaagcaaaaa
     2881 tagcaccttg cagttgatgg aagaatcgat ccaagtcgat caaaatgctc agcggatcag
     2941 tgccagtgac cgcgcaaccg tattgaatct gctgtccgtg acgaatggtt acttggtcgg
     3001 atccggtctc ctgaaatcaa acacgcagga ccatatcgtc gtggtgccga tggatgtcga
     3061 tcaaaaaaat accatcggct tcatttattc gaaagtgcgt ccgctcagca aaatctccaa
     3121 gcgctacatg gaaatcgtaa gggaactgtt ggagggagat caacgcatcg aactgacgga
     3181 tcatgtagac tgaaacatcc atattaccgg ttgtccgatt ctatgcggat atcggacaaa
     3241 gaatcacaaa ttctaagccg caaaaatata gcagccaacc ccaaacaaac aggaggcagt
     3301 cccgcaagcg ggataacctc ctgtttgttc attctgcgaa cagcgaatcg atttccactc
     3361 gcagtacccg gtcgatgctt gggttgccat gggcgcggta agggtaagca cctgattcga
     3421 agagaatgta gagctgcttc ccgtccacgg caatctgttc cagataggac ggcatcgtga
     3481 tttcggtgct ggcgttcttg tcggtgaaat tttcggtttc cttcaagcga tcgaaagaaa
     3541 gcagcgtcga gtccttcttt ctgccggcag agcgcgacag catcagcttt tcgcgataaa
     3601 aagccagacc ttggatctct ttgccgatct tgtcgacatc gtcgggataa gcctcttctg
     3661 tgctttttcc tgtatcgagc gcatggactt cctttccgtc ctcgtcttct gttgtggaaa
     3721 tgaggtagcg gtgcatgaca cttgtctctt cgtcatcgaa atgcgctacg tagaggtcat
     3781 cgccgtggac cgtgattgtt gaagcgcgtt tgatgtcagg cagttcgatg ctgtccgtat
     3841 aggtaatcgg ttcagcggaa tccgcgaagt cataggcttc aatatccttc aaagacagag
     3901 tgatgacctg cgcagtgtcg cctttttccg aggtgaccca gagcagctca tggttgttgt
     3961 cgtaggcgag gccgcctgca tgcggaatgc cgtccaagac gatttccttg atgaaggaac
     4021 cgctcttttt ttcaagcaca tagatgacgg aattgtgttc tttggtttgg caataagccg
     4081 atatgaacag gtaatctttg ttgacggcca aaccttgcgg cgtcatggtg gtgcagatat
     4141 ccgcttcttc gtgttcatga tggagcgtca aagtggcatc cattcccggg atgatgaaaa
     4201 tgttgtcttc cggattgatg ccatcctctt caaaagccgc tcggtacagt tcgggatagt
     4261 cggacagtct ctccattagt tcatccagtg aaccaaggct ggcttccgtg ttcgtttctt
     4321 cggcaaaagt caaccgttcg atttttccgt tctttgtgaa gagaacgagt gtaagaatgc
     4381 ctgcaatcac gatgccgatg agggcgaact tcaattttcc cattttcctt gccatatgat
     4441 cacttcctaa actatatatt tcgacaaatt tgttattgta tatagtttac tataggagtt
     4501 tatcagcaac caacgaaaag cctctggtgc aaaggcaagt gaggaatatc tgtccttaca
     4561 aaagcatttt ttgatgcagt aaaataaaac tattgaagta ttgataatag ttgggaatgt
     4621 tatccagtct gaaatcgagt acctatccat aacaatgtag ataacgtata atagaaggac
     4681 gcaatcaggc gcagcgcaat cggcctgacg aagggatcaa cactaccaat attgaacaga
     4741 aagaggattt taccatgtcg aagttaggtt tgcagctcag cccggcagac agcgaaagca
     4801 aatgttgggt ggcggaaatt accggagcgg acgaagtata cattttaaaa agagacttca
     4861 tcccggccga accggaaggc ggatggatcc tctacgatgg ctggtaccag ctgaatggag
     4921 cggtgcccgg tgtcacggag ttcaaaaaag aatatatccg catcaaggac ggaaaagtca
     4981 gaaggaacct tccattccgg gaactcgtcg aaagcctgga tgaaataaaa gccggtgaag
     5041 gccctcgtgt tgaaaggatg cgcaaggaaa tcatcgccat tttggatgaa atcaaagaag
     5101 cggcctactg tgagcctgtg gtggagggca tcgaaaaaca aaaggaagat ttggacatgg
     5161 cggatgagcc ggatcaaatc aaaaacgcgc tctacatgct gaaaaaacaa aagcaatcct
     5221 atatccagca gtaccgaaag atgttcaacc tataagaatc cagattgtcg gggatgaaaa
     5281 caataaaaat gcaggaaaac aataaaattt ccggtataat cttatttaaa tggaaaaatg
     5341 gaaggcggtg gagcaatgat cagaaaagca atgccagcgg atctgcctgc aatcatggaa
     5401 atcatcaatg aggcgaaagt cacactgaag gcatccggca tcgatcaatg gcaaaaaggg
     5461 tacccgaacg aagcggtcat tcttcaggat atggccggag ggtacagcta tatctacttg
     5521 cgggacggta tcccagcggc aacttttgca tttttttacg gcgaggatcc tacctatgaa
     5581 gtcatcaccg aaggccaatg gctaacggat aatccttaca cggtcatcca tcgcatcacg
     5641 ataaaaggaa cattccgtgg tcagggcgtt cttggggaaa tcgtcgcatg ggctggggac
     5701 gaatcgcgta aaagaggcta ttccagtatg cgcatcgata cgcatcccga caacaaaagc
     5761 atgcagcggg ccctgcagaa agccggctac acctactgcg gccacatcct gacgagcatc
     5821 ggcgatatgc gttggggtta cgaaaagcta ctgtaaaaag aactctgcag attttcaaga
     5881 aggattgcgc ctgaccaaga ggcgcaatcc ttttcgcatg gaatggtcga ccagtggaaa
     5941 cgatgaggag ataatgaaat gaacgtgcaa ctaaaaaatt taccattgaa cgaggaactg
     6001 atccaggcag tgcgccgttg ggaaaatgac ccggctattt caccgttcat cacgccgcac
     6061 tatcttgaaa caagtgagct gccgttggtg acggaaagcg atgtccgcga gagcctgtcc
     6121 aatccggaca aacgcaatta tttcatctgc gtggatgaca agccgatcgg gattgcatcg
     6181 atcatccgtg actttcctgc cctgttgaag aatgacggga aaacagcttg gctgggaatc
     6241 tatatcggcg aaccggattg gcgcaacaaa ggcatcggca aaacggtcat ggctctgtat
     6301 gaagagaaat gccggagtct cggctatcgg cgcatcgaac tgggggtatt ttcccacaat
     6361 gccggcgcaa tcagattgta tgaaagatcc ggcttcacac aaataggcgt cattcctcac
     6421 ttcacctatt cacaaggcca atggcgcgac gacatccgaa tggaaaagat tctctgtcac
     6481 aatctaggat aaaatccatg cggcaatcta ttggagggag gaaaatcaac ggatgaaaaa
     6541 aggagtgctg gttagatcta aacgtatatt actgaccttc tttgcggcgt tgttggggct
     6601 gctgacacta ttcagctatc ataactacgc atggtacgaa gaaccgatcg ccaaaataac
     6661 cgcagcggaa gctgttggaa ccgatgaaac gattggtgca ggtcaggaaa actatcccat
     6721 atacaaccaa aaactgacgg ctgtactgat gaacggggag tcgaaaggga cgcaagtcgt
     6781 gatagaaaac aactattccg actcacttgc ctatagccag atgtaccgtt caggggatga
     6841 agtgttcctt tccttaagcg agcgtgcttc gggtaccctg acaggtaaaa tttccggaat
     6901 aaagcgggat aaatatgttg tcctttttgg atccttattt cttttcgtat tggtggcggt
     6961 agcggggaaa aaaggtctgc tttccctcgt cagtattgca gggaacatat tgctgttcaa
     7021 tatcgctttg gacagctatg aaattgcaga taacgcacag ctattatgga taagcagtgc
     7081 tgcagtgatc ggatggaccg tcctgacact gctcttggtc agcggccgaa acagaaagac
     7141 ttacgcggct atcctttcta ctttgatcgg aacattcctg actttgatca tcgcttatgg
     7201 ggtcattgcc gggacgaacg ggcaaggcct gcgctatgag gaaatgcagt ttgtgacgca
     7261 ttacccgcga cgaatcttcc tatccagcat attgatcgga tcgttgggtg cggtgatgga
     7321 tgtcgccatt acgatgaccg cggccattca ggaactgtac gaaaaagacc ccgcaatatc
     7381 cgccaaagct ttattccagt cgggacgcga aatcggcaag gacatcatgg gaacgatggc
     7441 aaatgtgttg ctttttgcct atgtcagcgg agcgattccg acgctcgttt tttatctgga
     7501 gaacggctcc gatgtgttcc ataccttttc gatgcaatta tctttggaaa tcacccgagc
     7561 tttggcgggc agtttgggta tcgttttgac ggttccgttg gcaatgctca cagctgtccg
     7621 tctgattccc aagaaagcgg gtgatcggcg atgagcgtgc aactggtatt ggcgctgttg
     7681 cttttgctgc tgatggttgt agtgggcggc agacgtggta tttcttcgtt cctgtcgttg
     7741 tttttcaact ttgctgtcct ctatgtattg gtgttgctgt tgataaatgg ctttcctgca
     7801 cttgttgtta ccctgctggc ttgtgcggtg gtcagctaca tcaatttatt ctacataaac
     7861 ggcgtgaatg ttaaaacgaa ggcggccttt attgcgacga tcgttacgac tttttttttg
     7921 ttgttactga ctattgtcgt gcatcggttt gcgatgatcc aaggcttcgg tcccgaggaa
     7981 gtggaggagt acaccacgct ttcctttttt gtgaatgtta attttgttca aatcggaatt
     8041 tgtaccatga taaccggaac gatagctgcc attacggaca cggctatctc catttcatct
     8101 tcattgtatg aggtccataa ctgcaatccg cagttggggg aaaaagaatt attccgttcg
     8161 gggatgacaa tcggaaagga catcatcgga acaacgacga atacgctcta tttttctttc
     8221 gtcggtagtt atctcgcact ttttctctgg ttcagggacc tgtcttattc tttcggggag
     8281 gcgctgaacg caaaagtact cgtttcggaa gtcctttcca tgttcgtcat cggcatcgga
     8341 gtgaccggca ttatcccact caccgcctgg gttacggctc acatgttgtt gaacgaatca
     8401 tcgcgggatt aaaatgctta ttgccaatca aagaaattgc tctataatag ttagagttga
     8461 aaacaaagct ttttggatga ggcagatcat acagtttcgc atccgggaag agacgcataa
     8521 tagtaagtat acagatacac cattcagaca atcggaggaa aacagatgaa aaaaatcggc
     8581 tttgatccac agaagtacat tgaagagcaa tcgaaatata ttctcgaacg ggtcaacaat
     8641 tacgataaat tgtatcttga attcggaggg aaactgatcg gcgacaagca tgcgaaacgc
     8701 gtattgcctg gtttcgatga agacgcaaaa atcaaattgc tgcagaaact aaaagaccaa
     8761 gcggaaattt tgatttgtgt atatgcgggg gatatcgaac gcaataaaat ccgcggcgac
     8821 tacggcatca cttatgatat ggatgtcttc cgcttgatcg acgagctgag ggattatgga
     8881 ttatccgtca acagtgtcgt catcacgcgc tataacggac aaccggccac taaagtgttc
     8941 atcaacaaac tcgaaaaacg cgacatcaaa gtctataccc atggcgccat cgaaggttat
     9001 ccttccaata tcgaaaagat cgtcagcgaa gagggcttcg gccagaacac ttatattccg
     9061 acgaccaagc cgattgtcgt cgttaccgct ccgggtcccg gcagcggcaa gttggctacc
     9121 tgcttgaacc aactgtacca cgaacgccgt caagggcgcg tggccggcta ttctaaattc
     9181 gagactttcc cggtctggaa tgttcctttg aagcacccgc tgaacattgc ctatgaagcc
     9241 gcaacagtcg atctgaagga tgtcaacatg atggacaact atcattttga gacttacaac
     9301 gaagtggcag tcaactacaa ccgtgatctg gagactttcc cggtcatcaa acggatcatc
     9361 gagcgcatca ccggcaagga atccgtctac caatcaccga ctgacatggg cgtgaaccga
     9421 gtaggcttcg gtatcaccga tgatgaagcg atccgtgaag cgtcgaagca ggaaatcatc
     9481 cgccgcagct tcgacacgga atgcgactat aagaaaggtt tgagtgatga agagacgcgc
     9541 gatcatatgc gtctgatcat ggaagaactt gagctgaaac aggaagaccg cgcaccagta
     9601 aaacctgccc gcgcatacgc ggaacgcttg atgaaacgta cggttgacaa cgaaattcct
     9661 gcggtcatcg ctttcgaatt gaacgacgga agaatcatca ccggcaggac ttccgatctg
     9721 atggatgctt gttcctcagc catcttgaac gctatcaaag ccttggcgaa catttccgac
     9781 gacatcctgt tgttgtctcc ggtcattctc gagccaatca aaaagctgaa gagcgatggc
     9841 ttgcacaagc gcatcccgac attgactgcg aacgagatcc tgatcgcgct atccatatca
     9901 gcggtcacga atccgacagc tcagttggct tacgataaac tttccgagct gaaggatgtg
     9961 caagcccatt cgacggtcat ccttaacaaa gatgatgatc agatcctccg gaatcttggc
    10021 ctcgacatca cctgcgatcc ggtttaccca tccgagaatc tttactacgt ataatagagt
    10081 gtgatgaatc gcgtttagac acccgtacgt tggagaaacc agctgccgtt cacctctttt
    10141 gtggacgaca gccggttgcg aaacggtagc ggtgtctgcg aatcagaaca cgtttgcgat
    10201 aaatcgtcag aatccaattt caaaggctat ccgctcccta tcattttcgg gagcggatag
    10261 ctttttttcg gggctgatgc ggatcgttat tgtcattggt cctcaaaatg gatgcccctg
    10321 ggaccgaccg aggttgagaa gacgatctgc gtgtaggtct tgccgaactg ctgcagttcg
    10381 ccgatgaaag tatccaattc cttcgtcgtc ggaaaggcga ccttcaggag catcgaaaat
    10441 tctccggtca cgcagttgca ttccaacaca ttcgggatgc cttccacgaa tggataaaat
    10501 accggctttt gcttgggatc gacctccaga ttgatgaaag ctttgatgtt gtaacccagc
    10561 gcctgaaggt tcacgtgagc gccgtaatca gtgatggtgc cgttccgctc cagctgggcg
    10621 atccttgcgg aaatcgtcgg cgaagacaaa aaagtggcct caccgatttc cttcagcgaa
    10681 gcccgcgcat tcgtctgcag aatgttcaaa atttgtttat cgattttatc cattggaggt
    10741 tccttccttt ttcttgtttg ctttgtattc ttaagatttt atatcatacc tatcatggga
    10801 agcaaaccaa tactgacatg tcttaagaaa ataaagttat atcggaaaca gcatgggatt
    10861 cttctgtgaa aaggtcgaat atgcaggttt tgttcgcaac ttcttgtgag ttgttttaat
    10921 cagtttgtca aggtggtatt gtttcctcta tgaagtacag agaagtacag aaaaatttca
    10981 gggggcaaac agagatgaat atgagaattg aagcggactc gatcggaacg ttggcagtac
    11041 ccacagaagc gtattatggt gtgcaatcat cgcgtgcaaa ggaaaatttt gccatcacgg
    11101 gcacgaatct gcatccggaa ttggtccaaa gcttggcatg gatcaagaaa gccgcagctg
    11161 tcgtgaacgc tcaatccggg aacctgacag ccgttaaaaa ggatgcgatt gtccaagcct
    11221 gcgacgaaat tatggcggga tattttcatg aagccttcat cgtggatgcc atccaaggcg
    11281 gtgccggtac ttcagccaat atgaacgcca acgaagtcat cgccaatcgc gccatcgaaa
    11341 tattgggtgg gagaaaaggc gactaccgca tcgttcatcc aaatgatgat gtgaatcttt
    11401 gccagtccac caacgatgtc ttcccgacag cgggtaaaat gaccgtgctg caaattctgc
    11461 ctgagctgat tgccgaactg gaaaagttgg agagcgcatt gggtgaaaag gccgaagcat
    11521 ttgccggggt catcaagatg gggcgtacgc aaatgcagga tgcggtcccg actacgttgg
    11581 ggcggacgtt ccgggcttat caatccttcg tcaagaggga tatccgccac ctcatgagtg
    11641 ccgcggaaga gatgaagacc cttaatctcg gcgggaccgc aatcggtaat accatcaatg
    11701 cgacgccgga atacctggaa aacatcacag cttttgtgag cgaggaagtg gggattgctt
    11761 tcacgcaggc ggatgacctt ttcgatgcga cccagaacgt ggatggcttt gtcagggtct
    11821 cggcggcaat caagacggcg gcggtgaact tgtcgaagat gagcaatgat ctgcgcttgt
    11881 tgtcgagcgg accgaaggcg ggcatcggtg aaatcaattt gcctgccaag caaaacggct
    11941 cttcgatcat gccgggaaaa gtaaatccgg tgatacccga agtcgtttcg caagtcgctt
    12001 tccgcgtaat cggaaacgat ttgaccatca cgatggccgc cgaatcgggt cagctggagc
    12061 tgaatgcctt cgaaccgatc attttccatt cgctgtttga atcaatcgac tgcttgacgc
    12121 atgcagtcgc cactctaacc gccaattgca tcacagggat ccaagcgaat gagatgatct
    12181 gctacaaaca ggtggaggag agcgccggta ttgcgaccgc cctttgcccg acaatcgggt
    12241 accaaaaagc cagcgcaatc gccaaagaat cactggagac agggaaaaca gtcagggaat
    12301 tggtgcttga aaaaggcttg ttaacaattg cggaaacgga cagcatcctc gatctaaagc
    12361 acatggccgg catcccagct gcgatattgc tgagggataa agtcatctga gcgacaaatg
    12421 aaaagcaagc ccgggagatg atcctggact tgtttttttg ttggacatat aggaatttat
    12481 aaaatttttt caactc
//
LOCUS       DAOMUJ010000009        11876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1257_length_11876_cov_2.463582, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000009 DAOMUJ010000000
VERSION     DAOMUJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_11876_cov_2.463582"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="pstB"
                     /locus_tag="PLT73_00595"
     CDS             <1..238
                     /gene="pstB"
                     /locus_tag="PLT73_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRM18911.1"
                     /translation="PTSALDPISTSKVEELIQTLKKDYSIIIVTHNMQQAARISDQTA
                     FFLNGEVVEYDKTEIIYSNPNDKRTDDYISGKFG"
     gene            305..979
                     /gene="phoU"
                     /locus_tag="PLT73_00600"
     CDS             305..979
                     /gene="phoU"
                     /locus_tag="PLT73_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM18912.1"
                     /translation="MRKIFEEELQDLHVLFSEMGKMVNEAIYISVKGFVNHDKELAAE
                     VIAHDVAINQREAEIEKRCFELIALHQPVTGNLRRIVTIMKACADLERMADHAVSIAK
                     STIRVKGNKRNSDIEAAIAEQSEKVKVMVEEVLAAYIKTDIEKAKEVALSDKMVDQAA
                     EHIYGRAIEEMKLDPELVLGATDYIMVAGYLERIGDYVTNICEWIIYLGTGKIIELNT
                     NNKFED"
     gene            1356..2216
                     /locus_tag="PLT73_00605"
     CDS             1356..2216
                     /locus_tag="PLT73_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HRM18913.1"
                     /translation="MKLKYVSAIVSLAAVFALSACGPVVDEESITAVGSSALQPLVEA
                     AGEQFSAENLGKFVNVQGGGTGTGLSQIAAGAVDIGNSDIFAEEREGIDASQLVDHRV
                     AIVGITPIVTPSVGVTDLSMEELRGIFTGKYSNWSELGGKDLPIVVLNRAKGSGTRAT
                     FDKWVLAGEESIITQEQESNGTVRQIVASTPGTISYVSFSYVNEDVEALSVDGVKPTP
                     ENVTTNAWVIWAYEHMYTNGEPTGLTKEFLDYMLSEDVQGSLIEALGYIPSTDMKVDR
                     DIEGNVTPIE"
     gene            2315..3232
                     /gene="pstC"
                     /locus_tag="PLT73_00610"
     CDS             2315..3232
                     /gene="pstC"
                     /locus_tag="PLT73_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRM18914.1"
                     /translation="MTPGNLMKKSSKRRLESFGRTISLISTTVIVFIVLSILYFVISK
                     GISTFVTDGVSLGDFLTKSIWNPGTVADDGTPLVGALPMIAGSFLVTLISAALATPFA
                     VAAALFMTEISPKSGKKILQPVIELLVGIPSVVYGFIGLTVVVPFVRSLFGGTGFGIL
                     SSTLVLFVMILPTVTSMSVDALNAVPRHYREASLAMGGTRWQTIYKVVLRAATPGLLT
                     AVVFGMARAFGEALAVQMVIGNATLMPNSLVTPASTLTSILTMSMGNTVMGTLENNVL
                     WSLATILLLMALFFNILTRYIGKKGAVKK"
     gene            3235..4119
                     /gene="pstA"
                     /locus_tag="PLT73_00615"
     CDS             3235..4119
                     /gene="pstA"
                     /locus_tag="PLT73_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM18915.1"
                     /translation="MNPKKVDKFMVGLIYLFSGTIILILASLLLFILWRGLPQFSWEF
                     LTAPAKSFQVGGGIGIQLFNSFYLLVLTLLISTPLALGAAIYLSEYAPQNWFTDIIRT
                     AIEVLSSLPSVVVGLFGFLIFVIQYGFGFSILSGALSLTIFNLPLLTRTIEDSLRAIP
                     SSQREAGLALGLSRWETVTLIILPAALPGILTGMILAAGRIFGEAAALIYTAGQSAPA
                     LDFTDWNPQSITSPLNIFRPAETLAVHIWKINSEGVKPDGAEVSAGASAVLVLAVLIF
                     NLTARSLGRRLQKKATSA"
     gene            4153..4959
                     /gene="pstB"
                     /locus_tag="PLT73_00620"
     CDS             4153..4959
                     /gene="pstB"
                     /locus_tag="PLT73_00620"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM18916.1"
                     /translation="MGNEKILATNIIQMRPEAEVALKTNDLHVHYGDFEAIKGVSMEF
                     EKNKITSLIGPSGCGKSTYLRSLNRMNDTVPGAKVTGEIIYQGIDVNVPEVDVFEMRK
                     NIGMVFQRPNPFSKSIYENIAFALRRHGLKDKATLDEIVETSLKQAALWDQVKDQLNK
                     SALALSGGQQQRLCIARAIALKPDILLLDEPASALDPISTSQVEETLLELKEKYTIII
                     VTHNMQQASRISDYTAFFYLGNVIEYDETRKIFTRPKIQQTEDYVSGHFG"
     gene            4972..5736
                     /gene="pstB"
                     /locus_tag="PLT73_00625"
     CDS             4972..5736
                     /gene="pstB"
                     /locus_tag="PLT73_00625"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM18917.1"
                     /translation="MKSTKPIIETRDVHLYYGKNEALKGISLDFHKKEITALIGPSGC
                     GKSTYLRTLNRMNDLIPDVTVTGKISFNDQDIYSPQMDTVELRKKVGMVFQQPNPFPF
                     SVFDNVAYGLRIAGMKDKEKIAQIVEESLKKAAVWDDVKDKLDQSALSLSGGQQQRVC
                     IARVLAIEPEVILLDEPTSALDPISSAQIERMLMELKEDYTMIIVTHNMQQASRISDN
                     TAFFLNGELIEYGETSQIFTNPKEKQTEDYISGRFG"
     gene            5780..6448
                     /gene="phoU"
                     /locus_tag="PLT73_00630"
     CDS             5780..6448
                     /gene="phoU"
                     /locus_tag="PLT73_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM18918.1"
                     /translation="MRRVFEEELESLHAHFLKMGVLVNAAIHKSIDAFVTHDKELAQQ
                     VIDEDAAINKLENDLEIECFELIALQQPVTTDLRKIVTVMKASADLERIGDHAVSIAK
                     STIKVKGKKHDADIETAIAETALKVMDMVKEVLDAYVVLDVERAREIAARDTEIDAAA
                     KNIYENCIEQMKQDPEIVLGGTNYMRISTYLERIGDYVTNISEWIIYLDSGEVVELNV
                     HHKI"
     gene            6633..6980
                     /locus_tag="PLT73_00635"
     CDS             6633..6980
                     /locus_tag="PLT73_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM18919.1"
                     /translation="MSDLNTETIQAVTKIFKLIGDPTRFLILHVLENRELNVNAIAEE
                     LDLEQSAVSHQLKKLREAKLVKSRRNGKNILYSQEDQHVYAILHMAVEHAEHGREDAK
                     IKISTTASDKKPH"
     gene            7086..9935
                     /gene="uvrA"
                     /locus_tag="PLT73_00640"
     CDS             7086..9935
                     /gene="uvrA"
                     /locus_tag="PLT73_00640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM18920.1"
                     /translation="MAKDQIIVRGARSHNLKNIDVTIPRDKLVVMTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAIAIDQKTTNRNPRSTVGT
                     VTEVNDFLRLLFARVGHPICPNDGTPISSQSPEQMVNQVLEFPERTKMQILAPIVKGK
                     KGQHKKVFEDIKKQGYVRVRVDGELFDISEDIELEKNKKHDIAIVVDRVVIKEGIRSR
                     IFDSIEAALHLADGYAIVDVIGSEELLFSEHHACPYCGFTIGEIEPRLFSFNAPYGAC
                     SECDGLGMKLEVDLDLIVPDKQLSLNEGALVPWNPISSNYYPEMLRQFCAQNGIDMDL
                     PFAELGPDTQQKLLYGAGGDLFHFHYQNEFGGVRDVDIPFEGVITNVNRRYKETSSDF
                     TREAMRQYMTELPCHTCHGYRLNEQALAVKVAQHDIGQVTEFSIENAVAFFEGISLSE
                     QEQQIAAPILKEILSRLTFLKNVGLDYLTLSRTAGTLSGGEAQRIRLATQIGSNLSGV
                     LYILDEPSIGLHQRDNNRLIESMKNMRDLGNTLIVVEHDEDTMMQADYLIDIGPGAGE
                     LGGEIVAAGTPEQVMKNKKSLTGKYLSGKLFIPVPETRRTEDKGAVKITGATENNLKN
                     VDAAFPLGKIVAVTGVSGSGKSSLVNNVLKTALAKELTRTKEKPGKFKTISGFEGLEK
                     IIDIDQSPIGRTPRSNPATYTSVFDDMRDLFASTNEAKIRGYKKGRFSFNVKGGRCEA
                     CRGDGILKIEMHFLPDVYVPCEVCHGTRYNSETLEVQYKGKNIAQVLDMTIEEALTYF
                     EAIPKIRRKLQTIVDVGLGYVTLGQPATTLSGGEAQRMKLASELQRVSSGKTFYILDE
                     PTTGLHTHDIARLLQVLDRLVEAGNTIVVIEHNLDVIKTADYIIDMGPEGGAGGGTVL
                     ATGTPEEIAEVEGSYTGQFLKPILERDKVRDKKNK"
     gene            10285..11796
                     /gene="liaX"
                     /locus_tag="PLT73_00645"
     CDS             10285..11796
                     /gene="liaX"
                     /locus_tag="PLT73_00645"
                     /inference="COORDINATES: protein motif:HMM:NF038025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HRM18921.1"
                     /translation="MNERDRILALVREGIITTEEALVLLENLAKREGKDAVKFNQQQD
                     FTDVPDTSSESSEQTAPVSDKEREDRRNLEKILDELASEISYFSSQIDAKGEALQSLQ
                     KQINDKSEKRQELATADELGDLTGEQEITLMRLDEELEALRGQVAALEAEKSEMEQKM
                     RSLKKEHVENSVKSFGEKLGNKEEWKDTATNFAERLTKAGTQLSKFLVDTVQTVAENV
                     DWKDTDFNIKIPGLITSKFQHEFIFDQNAATILDFQLANGNVTLQSWDNETISVAADV
                     KIYGKTEEATAKEAFDARSTIVMDEDKLTFRVPNKRVKCDIVVSLPKREYDYVAIKML
                     NGNATLKNIEGKDFYIKSTNGNMVISGLKAIMLETDGVNGNLDVSESTIVDVLAKTIN
                     GSLTIKSDVVSATVSVVNGDVKLSYPGTNAKQIQASSVNGSVKLSLPAAKSAEVKASS
                     SLGKIMNRMENIEILRQKNEHTNKYLEFRRMDEESPVMVELKTTTGNIFLKNN"
BASE COUNT         3454 a         2524 c         3027 g         2871 t
ORIGIN      
        1 gccgacttcc gcattggacc cgatttcgac gtccaaagtc gaagaactta tccagacttt
       61 gaaaaaagat tacagcatca tcatcgttac ccacaatatg caacaggcag cccggatttc
      121 ggaccaaacg gccttctttt taaatggaga agtggtagaa tatgataaga cggaaatcat
      181 ttactccaat ccgaacgata aacgcacaga tgattatatc tctggcaaat tcggttaaga
      241 ctgggctata atgggataag agatgcactg tgatgcataa tgacatgtaa cagggggcga
      301 aaagatgaga aaaatttttg aagaagaatt gcaggatctg cacgtacttt tctctgaaat
      361 gggaaaaatg gtgaacgaag caatctacat atctgttaaa ggttttgtga atcatgataa
      421 agagcttgct gcagaagtga ttgcgcatga tgtcgccatc aaccaaagag aagcggaaat
      481 cgagaaaaga tgctttgaat tgattgcgtt gcatcaaccg gtcaccggca atctccgcag
      541 aatcgtcacc atcatgaagg cgtgcgcgga cttggagcgt atggcagatc atgccgtcag
      601 tattgcgaag tcgactatcc gcgttaaagg caacaagcgc aattccgata tcgaagcagc
      661 gatagccgag cagtcggaaa aagtaaaagt catggtggag gaagtacttg ccgcctacat
      721 caaaacggac atcgaaaaag ccaaagaagt tgccctgagc gacaaaatgg tcgatcaagc
      781 tgccgaacat atctacggac gcgccatcga agagatgaag ttggatccgg aacttgttct
      841 gggtgcgacc gattacatca tggttgccgg ctatctcgag cgaatcggcg attatgtaac
      901 aaatatttgt gaatggatca tttatttggg tacagggaaa ataatcgaat tgaacacaaa
      961 caacaagttt gaagactaac acaattatga atgcaggatg agaaaagata aagccaggaa
     1021 ggccacgcaa tcgaacccga ttacgtggta gcttcctggc tttttttatg cgtttttgga
     1081 tcacggcagt caactgcagg tcattgaaaa tactcatgaa aatgtaatat atatttctgc
     1141 accagatata agacaagcgt tccgccaatt tgttataatg tggataatca gagcgtaaat
     1201 ttacagaaaa aaaacgtgaa atcaacgtta aaatgttcca tcgataaaat atatggtgga
     1261 tttacataaa atttacaaaa gagatacgaa acatttacaa acattcgtta cgatggataa
     1321 gtatgtgttc aaggtaaata aatgggaggt acacaatgaa attaaaatac gtttcagcca
     1381 tagtcagtct cgctgccgtt ttcgctctgt cagcctgcgg ccccgtagtc gatgaggaat
     1441 ccatcacggc ggtcggttct tctgcgctgc aaccgttagt cgaggccgct ggggaacaat
     1501 tcagtgcgga aaatctgggg aaattcgtca atgtccaagg cggcggtacc ggaacaggct
     1561 tgagtcagat tgccgcaggt gcggttgaca tcggcaattc ggatattttt gctgaagaga
     1621 gggaaggcat cgatgccagc caattggtcg atcatcgtgt cgccatcgtc ggcatcacgc
     1681 ctatcgtgac accaagcgtc ggtgtgaccg atctttccat ggaggagttg cgcggcattt
     1741 ttaccggcaa atacagcaat tggtccgagt tgggaggaaa agatctcccg atcgtcgtct
     1801 tgaaccgagc caagggcagt ggaaccaggg caacattcga taaatgggtt ctggccggag
     1861 aagaaagcat catcacccaa gagcaggaat ccaacggaac cgtccgtcag attgttgctt
     1921 caacaccggg aaccatcagt tatgtatcgt tctcgtacgt caatgaagat gtcgaggcgc
     1981 tttccgtcga cggggtgaag ccgacgcctg agaacgttac gacaaacgca tgggtcatct
     2041 gggcatatga acatatgtac acgaatggcg aaccgacagg cttgacgaaa gaattcctgg
     2101 attacatgct gtcggaagat gtccaaggaa gtctgattga agcgttggga tacattcctt
     2161 ccacagatat gaaggtcgac agagacattg aaggaaacgt aacaccgatc gaataaaaca
     2221 actgaaatat cattgtgatg aaaggcgtcc gccttttcgt aggaaggcgc ctttcgtcgc
     2281 ggctcaaagt ttaagaggag ggtttctctt ggaaatgaca ccaggaaact taatgaaaaa
     2341 gtcaagtaaa cgcagattgg aatcgtttgg acgcaccatc agtctgatca gtacgacagt
     2401 catcgtcttc atcgtgctgt ccattctata ttttgtcatc agcaaaggga tatccacctt
     2461 tgtcacggat ggtgtatcat taggggattt tttgacgaaa agcatctgga atcccggcac
     2521 cgtcgctgat gacggcacac cattggtcgg agccttaccg atgatcgcag gctcattttt
     2581 ggtcactttg atttccgcag ccttggcaac accttttgcc gttgctgcag ctttgttcat
     2641 gacggaaatt tcaccgaaat ccgggaaaaa aattctgcag ccggtcatcg aactgcttgt
     2701 cggtattccg tccgttgtat atggtttcat cggcttgact gttgttgtgc cgtttgtccg
     2761 ttccttgttc ggcggtaccg gatttggtat tttatcttct actctggttc tgttcgttat
     2821 gattttgccg actgtgacca gtatgtcagt ggatgctttg aatgctgttc cgcgtcatta
     2881 ccgtgaggct tcactcgcta tgggcgggac acggtggcag accatctata aagtggttct
     2941 gcgggcagcg acccccggat tgttgacggc agtcgtcttc gggatggcgc gtgcgttcgg
     3001 agaagccttg gcggttcaaa tggtcatcgg aaatgcgact ttgatgccga acagcttggt
     3061 gaccccagct tcgacattga caagtatttt gacgatgagc atggggaaca ccgtaatggg
     3121 aactttggaa aacaatgtcc tctggtcatt ggcgaccatc ctccttttga tggctctatt
     3181 cttcaatatt ttgacacgtt acatcggtaa gaaaggagcc gttaaaaaat gaatctgaat
     3241 cccaaaaaag tagacaaatt tatggtcggc ctgatttatt tgttttccgg aacgatcatt
     3301 cttattttgg ccagtctgct gctctttatt ctatggcgcg gcttgcccca gttttcgtgg
     3361 gaatttttga cggctccagc aaaatccttc caggtaggcg gaggaatcgg aattcagttg
     3421 ttcaattcgt tctatttgtt ggtcctgacc ttgttgatca gtacgccact tgccttgggc
     3481 gcggcgattt atctttcgga atatgcacct caaaattggt ttaccgacat catccgtact
     3541 gctatcgaag ttttgagttc tttgccatcc gttgtcgtcg gtctcttcgg attcctgatt
     3601 ttcgtcatcc agtacggttt tggcttctcg atcctttcgg gagctttgtc cctgactatc
     3661 tttaatctgc cgcttttgac gcgtacgatc gaagattcct tgcgggcgat cccgtcttcc
     3721 cagcgtgaag ctggcttagc gttgggcctt tcccgttggg agacagtgac gttgatcatt
     3781 cttccggctg ccttgccggg tattttgact ggaatgattt tggcggcagg acgtattttc
     3841 ggtgaagctg ccgctttgat ttatacagcc gggcagagcg cgccggcttt ggatttcaca
     3901 gactggaatc cacaatccat cacaagtcct ttaaatattt tccggcctgc cgaaacattg
     3961 gctgtgcaca tctggaagat caacagtgaa ggcgtaaaac cggatggagc ggaagtgtca
     4021 gctggtgcct cagcagtact ggtattggct gtcctcatct tcaatctgac tgccagaagt
     4081 ttgggcagaa gattgcagaa gaaggctact tcagcataga aacggaaaat tgacttgaag
     4141 gagggaccct tgatgggaaa tgaaaagata ttggcaacga acatcatcca gatgcgtcct
     4201 gaggccgaag tcgccctcaa aacaaatgat cttcacgttc attatggcga ttttgaagcg
     4261 atcaaaggcg tttccatgga atttgaaaag aataaaatca cctcgttgat cggaccgagt
     4321 gggtgtggaa aatcgactta tctccgttcg ttgaaccgaa tgaacgacac tgttcccggt
     4381 gcaaaagtga ctggggaaat catttatcaa ggcatcgatg tgaacgtgcc tgaagttgat
     4441 gtgttcgaga tgcggaaaaa tattgggatg gtattccaaa ggccgaatcc tttcagcaaa
     4501 tcgatttatg agaacatcgc tttcgcactg agaaggcatg gtctgaagga caaggcgaca
     4561 ttggacgaaa tcgtcgaaac cagcctgaaa caagctgctc tttgggacca agtgaaggat
     4621 cagttgaaca agagcgcctt agcgctctct ggtggtcaac agcagcggct ctgcatcgca
     4681 agggcgatcg ctctgaagcc ggatatcctg cttctggatg agccagccag tgcgttggac
     4741 ccgatctcga cttcccaagt cgaagagacg ttgttggagc tgaaggagaa gtatacaatc
     4801 atcattgtta cgcacaatat gcagcaggca tcccgaatca gtgattatac agcgttcttc
     4861 tatttgggca atgtcattga atatgatgag accagaaaaa tctttactcg tccgaagatc
     4921 caacagacgg aagattatgt ttctgggcat ttcggctagg aggaaagcaa cgtgaaaagc
     4981 actaaaccca ttattgaaac gcgtgatgta catttatatt acggaaagaa cgaagcgttg
     5041 aagggcattt cgcttgattt tcataaaaaa gaaatcacgg ctttgatagg gccgagcgga
     5101 tgcggaaaat ccacctattt aaggaccttg aatcggatga atgatctgat tccggatgtg
     5161 acggtcaccg gaaaaatcag tttcaatgat caggatatct atagtccgca aatggacacg
     5221 gtagagctga gaaaaaaagt cggtatggtt ttccaacagc cgaatccatt cccgttttca
     5281 gttttcgata acgtggcgta tggtctgcgc atcgccggga tgaaggataa ggaaaaaatc
     5341 gcccaaatcg tggaagagag cctgaagaaa gcagctgtat gggatgatgt caaagataaa
     5401 ctggatcaga gcgccttatc tttatctggt ggccagcaac aacgtgtctg catcgcacgg
     5461 gtcttggcga tcgaaccgga agtcattttg ttggatgaac cgacgagtgc cttggatccg
     5521 atttccagtg cacaaattga acggatgctt atggaattga aagaagatta cacgatgatc
     5581 attgttacgc ataatatgca acaagcatcg cggatctctg ataacaccgc ctttttcttg
     5641 aacggtgaac tgatcgagta cggggaaaca agccaaattt tcacgaatcc aaaagaaaaa
     5701 caaacggaag attatatttc tggacgtttc ggctgatcca ttgcataatg aaatatattt
     5761 acaaaggggt tggtttttga tgagacgagt gtttgaagaa gaattagaga gtttgcacgc
     5821 tcactttttg aagatgggtg tattggtcaa tgctgcaatc cataagtcaa tcgatgcttt
     5881 tgtaacgcac gacaaggaac tggcgcagca agtcatcgat gaagatgcag ccatcaataa
     5941 gcttgagaac gatctggaaa tcgaatgctt cgagctgatt gctttgcagc agcctgtgac
     6001 tacggacttg cgcaaaatcg tgaccgtgat gaaagccagc gcagacttgg agcggatcgg
     6061 cgatcatgca gtcagtatcg caaaatcgac gatcaaggtg aagggcaaga agcatgatgc
     6121 cgatatcgaa acggccattg cagaaacagc tttgaaggtc atggatatgg tcaaggaagt
     6181 cctggacgct tatgtcgtcc ttgatgtcga acgtgcgcgg gaaattgcgg cgcgggatac
     6241 ggagatcgac gcggccgcca aaaatatcta tgaaaactgc atcgaacaaa tgaagcaaga
     6301 tcctgagatc gttttgggcg gaacaaacta tatgcgtatc tccacttatt tagaaagaat
     6361 cggagattac gtaacaaata tttccgagtg gatcatttac ttggactccg gagaagtagt
     6421 agaattgaat gtccatcata aaatttaatg ataaaagcgt tgtgataccc aaagcggtgt
     6481 tgcaacgctt tttttctgtg tcagtcagag cttgagccaa gccggattgc atacagttgc
     6541 ttgattttgg cagcaagggc aatggctttc atagaacgga tgaatttgct aaaataagga
     6601 ttgaagtaag ggataggggt ctaagtgaga tgatgtcaga cttgaataca gaaacgatac
     6661 aggcagttac aaaaatattc aaattgatcg gcgatccaac gcgttttttg attctccatg
     6721 tactggaaaa tcgggaactg aacgtaaatg ctattgcgga ggaactggac ttggagcaat
     6781 ccgcggtgtc gcaccaactg aaaaagctga gggaagcaaa gttggtgaag agcagaagaa
     6841 acgggaaaaa catcctgtac agccaagagg atcagcatgt ctacgcaatt ctccacatgg
     6901 ctgtcgaaca tgccgaacac ggccgggagg atgcaaaaat taaaataagt acaacagcat
     6961 cagataagaa accgcattga acaggggagc gaactagtgt tcttttcagg gctggatatg
     7021 gtatactaca gtcagacagg aaatcgacga aaaccaaaga ataagtcaaa aaaggatctg
     7081 aaattatggc aaaagaccaa atcatcgtac ggggagcccg ttcgcataac ttgaaaaata
     7141 tagatgtcac catccccagg gacaaacttg tcgtcatgac cggtctttct gggtcgggta
     7201 agagctcatt agctttcgat acgctttacg cagaaggtca aaggcggtat gtggaaagcc
     7261 tctcagccta tgcgcgccag tttctgggtc agatggacaa gccggatgtg gacagtatcg
     7321 atggcttgag tccagccatt gcaatcgatc aaaagacgac gaaccgcaat ccccgttcga
     7381 cagtcggaac agtcacggaa gtcaatgatt tcctgcgcct gctttttgca cgtgtgggtc
     7441 atccgatttg tccgaatgat ggcaccccga tcagcagtca atcgcccgag cagatggtga
     7501 atcaggtgct tgagtttccg gaacgcacga aaatgcagat attggcaccg atcgtcaaag
     7561 ggaaaaaggg acagcacaaa aaagtattcg aggacatcaa aaagcaaggc tatgtccgtg
     7621 tccgcgttga tggggaactg tttgatattt ccgaagatat tgaattggaa aagaataaaa
     7681 aacatgacat cgcgattgtt gtggatcggg tagtcatcaa ggagggcatc cgttcgcgca
     7741 tcttcgattc gatcgaagcg gcgttgcatt tggctgatgg ctacgccatt gttgatgtca
     7801 tcggcagtga agaactttta ttcagcgaac accatgcttg cccgtattgc ggatttacga
     7861 ttggcgagat cgaaccgcgg ctgttttctt tcaacgcgcc ctatggagcc tgttccgaat
     7921 gtgacgggtt aggaatgaag ttggaagtcg atcttgattt gatcgttccg gataagcaac
     7981 tttctttgaa tgaaggggct ttggtaccgt ggaacccgat cagttcaaat tactatccgg
     8041 aaatgctgcg tcaattttgt gcacagaacg gcatcgatat ggatcttccg tttgccgagc
     8101 ttggccccga cacccagcaa aaactgctgt atggcgctgg aggcgacttg ttccatttcc
     8161 attaccagaa tgaattcggt ggtgtccgcg atgttgatat cccctttgaa ggggtcatca
     8221 ctaatgtcaa tcgacgctac aaagaaacat cgagcgattt tacgcgcgaa gcgatgcgcc
     8281 agtacatgac cgagttgcct tgccatacct gccacggcta tcgtctcaat gagcaagcct
     8341 tggccgtgaa ggtggcccaa cacgacatcg ggcaagtaac ggagttttcc atcgaaaatg
     8401 ccgtcgcttt cttcgaggga atttctcttt cggaacaaga gcagcagatt gcggcaccta
     8461 tactgaagga aatcttgtca cgcttgactt tcctcaagaa tgtcggcctc gattatttga
     8521 cgttgagccg gacggcgggc acactttctg gaggagaggc gcagcggatc cgcttggcta
     8581 cacaaatagg ctccaatctg tccggcgtgc tctacatctt ggatgagccg tccatcgggt
     8641 tgcatcaacg cgacaataat cggttgattg aatcgatgaa aaatatgcgc gaccttggaa
     8701 acacgctcat cgtcgtggag cacgatgagg acacgatgat gcaggcggat tatctgatcg
     8761 atatcggacc gggtgctggc gaactcggtg gtgaaattgt ggctgccgga acgcccgagc
     8821 aagtcatgaa aaataagaaa tccttgaccg gaaaatatct gtcagggaaa ttgttcatcc
     8881 cggttccgga gacgcgaagg acagaggaca aaggtgccgt gaaaatcacc ggggctactg
     8941 aaaataacct gaagaatgtg gatgctgcct ttcctttagg gaaaatcgtg gcagtcaccg
     9001 gcgtgtccgg ctcaggaaaa agttccttgg tcaacaatgt cctgaagacg gctctcgcca
     9061 aagaattgac ccgtacgaaa gaaaaaccgg gcaagttcaa aacaatcagc gggttcgaag
     9121 gtctggagaa aatcatcgat atcgaccaaa gcccgatcgg acggacgccg cgaagcaatc
     9181 cggcaaccta taccagcgtc ttcgatgaca tgcgcgacct atttgccagc accaatgaag
     9241 ccaaaatccg cggctacaaa aaaggccgct tcagcttcaa tgtcaagggc ggacgttgtg
     9301 aagcttgccg cggcgatggc attctgaaaa ttgagatgca tttcctgccg gatgtttacg
     9361 tgccttgcga agtttgccac ggcacacgct ataattccga aacgttggaa gtgcagtaca
     9421 aaggcaagaa tatcgcccaa gtattggata tgaccattga ggaagctctg acatattttg
     9481 aagctatccc taaaatcagg cgcaaactac agaccatcgt cgatgtcgga ctcggctatg
     9541 tgacattggg acaacccgca acgactttat ccggtggtga agcccagcgg atgaaattgg
     9601 ccagtgaact gcagcgcgta tcaagcggca agacattcta catactggat gaaccgacca
     9661 cagggttgca tacgcatgat atcgctagat tgttgcaggt tttggaccgt ctggtggaag
     9721 ccggcaacac gatcgtcgtg atcgaacaca atctggacgt gatcaaaaca gccgattaca
     9781 tcatcgatat gggacctgaa ggcggagccg gcgggggaac cgtgctcgct accggcactc
     9841 cggaagaaat cgctgaggta gagggcagct ataccggcca attcttgaaa cctattctcg
     9901 aacgggataa agtaagggat aaaaaaaata aatagccaaa acaggctgcc tcatttaatg
     9961 atgagacagc ctgttcgtcg ttgctgtcag ataatttttt tgagtcaatt ttctataatt
    10021 tggaaatgta gcattgttct ccggcgaact gcccctcgcc ggagcttagg acagcttata
    10081 cgcactttct tttttcagca ggcaccctac tgtgtccgct agcttctagt catttctgct
    10141 gaagagaaaa tcaaacttaa gacctatgac aagaggaacg aacttgggca taataaggac
    10201 ataagcaata gatatccaga aataggcaaa ttgagtcccc tgtcagcgaa tagataaatg
    10261 gataatcatc aaggaggaac taccatgaat gaaagagacc gaattttggc gttggtaaga
    10321 gaaggcatca tcactacaga agaagccttg gtacttttag agaacttagc gaagcgcgaa
    10381 ggaaaagatg cagtgaagtt caatcagcag caagatttca cagatgtacc cgatacttca
    10441 tcagaatcct cagagcaaac ggctccagtt tccgataagg aacgggaaga ccgcagaaat
    10501 ttggaaaaga ttttggatga gctggccagc gaaatttcct atttctcctc tcagatcgat
    10561 gcgaaaggcg aagcactcca gtcattgcaa aaacaaatca acgacaaatc cgaaaaaagg
    10621 caggaactgg ccacagcaga tgaattgggc gatttgactg gcgaacaaga aattacgttg
    10681 atgcgtttgg atgaagaact ggaggctctt cgcggacaag ttgccgcgct ggaagccgaa
    10741 aaaagcgaga tggaacaaaa aatgcgcagc ctcaagaagg agcatgtgga aaatagcgtg
    10801 aaatcttttg gtgagaaact gggcaataaa gaggaatgga aagacacagc caccaatttc
    10861 gcggaacgtc tcactaaagc aggcacccaa ctaagcaaat ttttggtcga tacggtccag
    10921 acggtcgcag agaatgtcga ttggaaagac actgatttca acatcaagat tccaggcttg
    10981 atcacaagca aattccagca tgaattcata ttcgatcaga atgccgcgac aatccttgat
    11041 tttcaattgg caaacgggaa tgtgacgttg cagagttggg ataacgaaac catttcggtc
    11101 gctgcagatg tgaagattta cggaaaaacg gaagaagcga cagcgaagga agctttcgac
    11161 gcccgaagta cgattgtgat ggatgaggat aaactcactt tccgcgttcc gaacaaacgg
    11221 gtgaaatgcg atatcgtcgt ctccttgccg aaacgcgaat acgattacgt cgcaattaag
    11281 atgttgaacg ggaatgcgac cttgaaaaac attgaaggaa aagattttta catcaaatcc
    11341 acgaacggca atatggttat ttcaggtctc aaggccatca tgctggagac ggatggagtc
    11401 aacgggaatc tggacgtttc ggaaagtacg attgttgatg tgttggcgaa aacaatcaac
    11461 ggcagtctga cgatcaaaag cgatgtcgtc agtgcaactg tttccgtggt gaatggggat
    11521 gtgaaactgt cctatccagg aacaaacgcc aaacagatcc aagctagttc cgtcaacggt
    11581 tctgtcaagc tgtctttgcc tgctgccaaa agcgcagaag tgaaagcaag cagttcgttg
    11641 gggaaaatca tgaaccggat ggagaatatc gaaatcctcc gccaaaaaaa tgaacacaca
    11701 aacaagtatc tggaattcag acgaatggac gaagaatctc cggttatggt agaattaaag
    11761 acaacaacag gaaacatatt tttaaaaaat aactaaaaat agcgataata aatggcacaa
    11821 ataaggagag aacgaacatg aaaaaattaa ccaaatctgc tacagacaga caagtc
//
LOCUS       DAOMUJ010000010        11094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1419_length_11094_cov_2.566265, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000010 DAOMUJ010000000
VERSION     DAOMUJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1419_length_11094_cov_2.566265"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLT73_00650"
     CDS             <1..313
                     /locus_tag="PLT73_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM18922.1"
                     /translation="MVQRDRMLSVYMGNFIAIPHGTDAAKEHVKKSGICVVQVPDGVD
                     FGDEQEEKVATVLFGIAGVGDDHLELIQKIALYCSDVDNVVTLADALTKDEITGSLAI
                     A"
     gene            326..1474
                     /locus_tag="PLT73_00655"
     CDS             326..1474
                     /locus_tag="PLT73_00655"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRM18923.1"
                     /translation="MKAVHFGAGNIGRGFIGEILNHNGYAITFVDVNDTIIEALKTRG
                     GYTIELADESRKQIQIENVTGLNNAKEAEKVVEAIVEADILTTAIGPNILPRIAQLIA
                     EGIKARAVRNIQQPIDIIACENMIGGSTFLAGEVKKYFTDTAYLDAYVGFPDAAVDRI
                     VPMQQHADPLFVQVEPFSEWVVQGTACKSPIRLEGVAYVNDLEPYIERKLFSVNTGHA
                     TVAYTGALQGYETIDEAMQDNLVVIQLRSVLHETGKLLIAKWGFDAAEHEKYIEKIIG
                     RFQNKYISDAISRVARTPLRKLGNHERFIRPMVELTQIDEMPFHLLETIGMVFNYFDP
                     EDEQCQELKEMILQKGLDKIITEVTGIDNQKILGSIKQNVEKYAYKVA"
     gene            1927..3728
                     /gene="glmS"
                     /locus_tag="PLT73_00660"
                     /pseudo
     CDS             1927..3728
                     /gene="glmS"
                     /locus_tag="PLT73_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
     gene            complement(4062..4328)
                     /locus_tag="PLT73_00665"
     CDS             complement(4062..4328)
                     /locus_tag="PLT73_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRM18924.1"
                     /translation="MGYSVKIAKSAMKDLKNLKSAHLDQKFIGLMEILKVNPSQNPLP
                     YEKLVGNLNDKYSRRLNIQHRVVYSVDIATKEVIIWSAWTHYER"
     gene            complement(4313..4567)
                     /locus_tag="PLT73_00670"
     CDS             complement(4313..4567)
                     /locus_tag="PLT73_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM18925.1"
                     /translation="MSIVNPSQARKIFYQLLKDVNETHEPVYISGKKEESEAVMVSKK
                     DWDAIQETLYLQSSGVADVIHQREQEHEFVALGDIDWDTL"
     gene            complement(4611..4871)
                     /locus_tag="PLT73_00675"
     CDS             complement(4611..4871)
                     /locus_tag="PLT73_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18926.1"
                     /translation="MKIIFDGLIPGKARHEWGKLIFYACGFQRSKTFSSENSELVQLL
                     LHCSAIIFELIRPCKVEKIHQENHKLRNFSAIHFGTPFYVLN"
     gene            5006..5809
                     /locus_tag="PLT73_00680"
     CDS             5006..5809
                     /locus_tag="PLT73_00680"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase subunit"
                     /protein_id="HRM18927.1"
                     /translation="MANNWLELDGKTVIVTGGNSGIGLHIADHLKEQGANVVVADLSV
                     ETGTSAYGKLNVKTDVTSIESIEAMVEKAVEKFGKIDVLVNNAGMNLPRLLVDVYSDE
                     RKYEIDEDSFNKMTAVNQKGMVFTTQAVVRNMLKNKVEGTIINISSESGMEGSVGQSI
                     YSATKGATNSYTRSWAKELGKFGIKVVGIAPGINEKTGLTTPAYNEALAYTRNIPVEQ
                     LDTGYEKSIPLGRVGKLDEIGYLVAFLSSNKSAYITGTTINITGGKSRG"
     gene            5825..7705
                     /locus_tag="PLT73_00685"
     CDS             5825..7705
                     /locus_tag="PLT73_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM18928.1"
                     /translation="MSTANDRELLLRSLLSNKETDLQSLEEASGKNKYQIKTIISELN
                     DVYEGFLKIQQKDESKVGIVRKEGANIFNTLHYNIHQDFNKVECRIAYLLYRLICTEE
                     YLNIGDLSEEMSVSRSTVNNDLKKLKQLSEKYNAKVVGIPNKGITFQCSEFGTRLVLI
                     YEVFELLQMTFLPDQEIQDALDGLVRDYKLDEQAKELLYKALAVSVHRLAHGHTIDSE
                     IPMYKNFEADSANIIAYTQLLQKKFAVALNDHEIAFLAFPINTRNSAYVKGSENSENE
                     EVLRQVVSSMLKTVRDQVMIEIDEEAFFDKVKYHLLFLINRLVFRIPNTDLYLEQIKL
                     KYPLAFELAKISLSELNRLYQLRASADDISYLAVYYALMLDERQANDSKGKSTKDVGI
                     ITNLGRGSFELICRQIKEIVGSEMNIVHINTDSLPKEKLKRFRLLFTTEEISVDTEVP
                     VIKIDRLVSPDDLAQKIYAVEKDQYNIKYLTNEDISYHLVNLKGQMGYFDYVEEIMSY
                     LSRRYRLSEDILDRFVEKEERHTMIYENKVAFPHLTDPNINKLVFVLGNITMANRNDA
                     FSLMLFLVTPEKISEQQERILMQVYDFVFKVINDPTLINDLKKVDDAQGLIEMLEKRG
                     TK"
     gene            7706..8158
                     /locus_tag="PLT73_00690"
     CDS             7706..8158
                     /locus_tag="PLT73_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HRM18929.1"
                     /translation="MNNMLALGILVVGAFLIQSALGFFQIRHFGNEYNELKANGRVAI
                     GKRAGKLTSGTIIMFQLDSNGMIVRGKKLQGTTILARFKPYNNFNGRLIEAIKAEDSD
                     VLAEIKITRKTIMNAVTNYRMFINGEIIPEKESPFKELTKKITNIKFG"
     gene            8199..8747
                     /locus_tag="PLT73_00695"
     CDS             8199..8747
                     /locus_tag="PLT73_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HRM18930.1"
                     /translation="MDFLVKGAESFIGLFQLGANTFMSWMTGIVPIVLMLMVFMNVVI
                     KLIGEERINSLAQRFSKYSLVRNCILPFLAAFMLGNPASFTLGRFLPEFYKPSFYAAL
                     AQYNHTSNGLFPHINAGELFVFLGIASGVETLGLSTTPLAIRYLLVGLVMNAVGAYVT
                     DFTTAYVCKKSGIQLSKTAKVQ"
     gene            8811..9818
                     /locus_tag="PLT73_00700"
     CDS             8811..9818
                     /locus_tag="PLT73_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol/sorbitol-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HRM18931.1"
                     /translation="MTKYNSIKVEKGSGGWGGPLIITPTEQKHKFIYIVGGGEKPAIV
                     DRIAELTGMEAVNGFKTSIPDDETALAIIDCGGTLRCGIYPQKGIPTINVVATGKSGP
                     LAKYINEDIYVSNVGLNQIQVTGETANIETVSTAVAEPVVEEKVAYTTDKKLTQQNAE
                     KGSVVAKIGLAAGKGIAIMLQSAREAVQTVLQTIIPFMAFVAMLIGIIQGSGIGNLFA
                     KMMIPLAGNVWGLMVIGFICSLPILSPLLGPGAVIAQVIGTLIGVEIGKGNIEPSLAL
                     PALFAINTQNACDFIPVGLGLQEAEAKTVEVGVPSVLYSRFLNGVPRVFVAWLASFGL
                     Y"
     assembly_gap    9859..9868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10145..10513
                     /locus_tag="PLT73_00705"
     CDS             10145..10513
                     /locus_tag="PLT73_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HRM18932.1"
                     /translation="MSIYSTVVTEIGPNAKEFLAEDMMVLFGDDAPEALRPYCFLIEQ
                     NALEQDIQLGHALVIGDDSYRVTAVGNVVNKNLRDLGHITINFSGETVAELAGSLYVE
                     HKTVNEIAEGTIIKITETVG"
     gene            10520..>11094
                     /locus_tag="PLT73_00710"
     CDS             10520..>11094
                     /locus_tag="PLT73_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRM18933.1"
                     /translation="MYIDSNDIKAIGSFLKSGFLKGVTTNPTILSTGSGSRFKQLQDL
                     LALEPTELFVQLLGTTSEEMFADYEAIKTFDANHRLSIKIPVTQAGLETIKKVKEDDP
                     ERAILGTLIYSADQGILAAAAGCDYLAPYVNRMLNNAIDPFKAIRSMRTFIDARGYKT
                     KIMAASFKNSQQVIDSLESGAHTATIPPDVL"
BASE COUNT         3345 a         2376 c         2670 g         2693 t
ORIGIN      
        1 tatggtgcaa cgcgatcgga tgttgtcagt gtatatgggg aatttcatcg cgattcccca
       61 tggtaccgat gcagccaaag agcacgtgaa aaaatcaggc atctgtgtgg tgcaagtgcc
      121 tgacggggtc gatttcggcg acgagcagga agaaaaagtg gcaaccgttt tgttcggcat
      181 cgcaggagtc ggcgatgacc acttggaatt gatccaaaaa atcgcgctgt attgcagcga
      241 tgtagataac gtcgttactt tggcggacgc tttgacaaaa gatgaaatca ctggcagctt
      301 agccattgca tagggagaga tgaaaatgaa agcagtacac tttggtgccg gaaatatcgg
      361 ccgcggcttt atcggggaaa ttttgaatca caacgggtat gcaatcactt ttgtggatgt
      421 gaacgataca atcatcgaag ctttaaaaac acgtggcggc tatacgatcg agctggcgga
      481 cgagtcacgc aaacagatcc agatcgagaa cgtcactggg ctcaataatg ccaaagaggc
      541 agaaaaagtt gtggaagcga tcgttgaagc ggatatctta acgactgcca tcggaccgaa
      601 tattctgcct agaatcgcgc aattgattgc agaagggatc aaggcacgtg cagtccggaa
      661 catccaacag cccatcgaca tcatcgcctg tgaaaacatg atcggcggat cgaccttcct
      721 ggcgggtgaa gtcaagaaat atttcacgga cacggcttac cttgatgcgt atgtcggctt
      781 ccctgatgca gcggtggacc ggatcgttcc gatgcaacaa catgctgatc cgctctttgt
      841 gcaagtggag cccttcagcg agtgggttgt acaagggaca gcctgcaaaa gcccgatccg
      901 actggaaggc gttgcctatg tgaacgatct ggagccttat atcgaacgca aattgttcag
      961 cgtcaataca ggacacgcta cggtagccta tacaggagct ctgcagggct atgaaaccat
     1021 cgatgaagcg atgcaggata atcttgtcgt catccaactg cgctcggtat tgcatgagac
     1081 aggcaaattg ttgatcgcta aatggggatt cgacgcagcc gaacacgaga aatatatcga
     1141 aaaaatcatc ggccgtttcc agaacaaata catttccgac gcgatttccc gtgtggcgcg
     1201 cacgccttta agaaaattag gcaatcatga gcgcttcatc cgtccgatgg ttgaattgac
     1261 ccaaatcgat gagatgcctt tccatttgct ggaaacgatc ggcatggtct tcaattattt
     1321 cgacccggaa gatgaacaat gccaagaact gaaagaaatg atcctgcaaa aaggattgga
     1381 caaaatcatt acggaagtaa ccggaataga caatcaaaaa atcctgggca gcatcaaaca
     1441 gaacgtcgag aaatatgctt ataaggtagc ttgatcactg cctttcaatc agcaactttc
     1501 agccatgccc gttgtgccgt tcaagccgtc attttagaca tcttgatgaa atcaaacggc
     1561 acaacgagta taagtttttc ttaaatagcg cttcaaaccg gctttgcgat tgaaaccgaa
     1621 tcaaaaacat tttgacaatg tcactgcttg taggataaaa tgcatatatt ctggaaaata
     1681 caaagctagg aggatagagg aagaagcaaa tggatagcgc cagacttcag ggagcacagg
     1741 taaattggcc ctgaagtgac gaggattggg tttatcgaaa gattcggcgg atgacccaag
     1801 gcaagtgtag tctacaggat tgaggtaaaa aagacccttt tgcggctgca gagcccagaa
     1861 cggtcttaca gagatcaatc cccacaccca gaaaaaaatc gagataaaaa ggggattttt
     1921 acgcttatgt gtggaatagt tggatatgta ggacaaggca atgtacaaga ggtattgttg
     1981 catggattag aaaaattgga ataccgtggt tatgattcag ccggtatttt tgtcgtagac
     2041 gctgaaaacc aaggtcatgt attcaaagaa aaaggccgca tcgctgattt gcgtgcaatc
     2101 gtcgacagac aggtggatgc gcatacagga atcggacaca cgagatgggc aacacatggc
     2161 gtgccgagcg cagagaacgc gcatccgcac caatctgcgg atggacgctt cactttggtt
     2221 cataacggtg tcatcgaaaa cttcaaagaa atcaaagacg agtatctgca gggcgttaat
     2281 tttgtcagcc aaacagatac ggaaatcgtt gtgcagttga tcgaaaaaat cgctgccgaa
     2341 ggaaatctgg acggaaaaga agctttacgc cgcgctttgt cgatcgtaag gggttcttac
     2401 gctttcgctt tagtcgatgc acaagctccg aatgtccttt atgcagctaa aaacaaaagc
     2461 ccgttgttga tcggcttggg tgatgacttc aacgtcgttg ccagtgatgc aatggctact
     2521 gttcagatca caaaccaata tgtcgaaatc cacgacggtg aaatgatcac attgaccgct
     2581 gatggcgtca caatcgaaaa attagacgga aaagcagtaa cacgtaatcc gtatacggct
     2641 caaatcgatg ccagcgactt ggaaaaaggg acataccctt actacatgtt gaaagagatc
     2701 gatgaacagc ctgctgttat gcgtaacatc atccaaaaat accaaaacgc agaaggtcgc
     2761 ttgactgttc cagaagaatt ggtcactgct atgttggagt ctgaccgcat ccacatcgtg
     2821 gcttgcggaa cgagctacca ttccggttgg gtaggcaaac attatctgga aaaaattgca
     2881 cagattccga cagaagtgca tgtcgccagc gaattctcct acaacccgcc gctattgaaa
     2941 ggtaatccgt tctttatctt ccttacccaa agtggggaga ctgcggatag ccgccaagta
     3001 ttggtgaaag taaaagaatg gggataccca gctttgacga tcacaaacgt ggctggttca
     3061 accttgtcgc gtgaagcgga ctacacattg ctgttgcatg caggtccaga gatcgcagtc
     3121 gcttcaacga aagcttacac ggcacaaatc gctgtcttgg ctgtcttgtc ggaggcgcga
     3181 gaacgggtca tgatatggga atcgacatgg tccatgaatt agggatcgtc gcaaatgcga
     3241 tggaagcggt catcgatgac aaagagcgca tcgctgcatt ggctgacatc tacttgccga
     3301 acacccgcaa tgctttctac atcggccgcg gtttggatta cttcgtatcg atggaagctg
     3361 ctttgaaact gaaagaaatt tcttacatcc aaacagaagg ttttgctgcc ggagaattga
     3421 agcacggcac gatcgccttg atcgaagaag gaactccggt cttggctatc attacccaag
     3481 ctgatgttgc cagccacacc cgcggaaacg tcgaagaagt gcgttcgcgc ggagcaaaca
     3541 cttgcgtatt tgcaatggaa ggtttggcta atgagaacga ccaaatcatc ttgccggctg
     3601 ttcacccagc cttggctcca ctgatgacag tcgttccgac ccaactgatg gcttactacg
     3661 caacgcttca ccgtggctat gacgtcgaca aaccaagaaa cttggctaaa tccgttacgg
     3721 ttgagtaaac agcatcttgt ttaaaaaaca tatatgagca gtatgggatc cttcagggat
     3781 tccatgctgt ttttttgcgt ttcttgtttg ggggtgcttg tgcttaccgg agcagcgcgg
     3841 aagcggtcct cagctccggt aaggtggacg gctaccggag aacaccggta atttcaaccg
     3901 caactccgac gaagctcttc ttatcggaga acgcaataaa atacgattgg aactccggcg
     3961 aagcctccgt tcaccggagg aacgtgccgg aacgtccgct aatccaatcc cggagagaag
     4021 tttcagaaaa aaagatagta gctgccttga tgcggcagct actatctttc gtaatgtgtc
     4081 caagcagacc agataatgac ttcttttgtg gcaatgtcaa cagaatagac tacccgatgt
     4141 tggatattta accgtcgtga atatttatca ttcagatttc cgaccaattt ttcataaggc
     4201 agtggattct gagaagggtt tacttttaaa atctccatta atccgatgaa tttttgatct
     4261 aaatgagcgg attttaaatt ttttaggtct ttcatagcgg attttgctat ttttacagag
     4321 tatcccaatc gatatccccc agtgcaacaa attcatgttc ttgttcacgc tggtggatga
     4381 catcagctac tccggaagat tgaaggtaca gcgtttcttg aatagcatcc caatcttttt
     4441 tgctgaccat aaccgcttcg ctttcttcct ttttgcctga tatgtagacg ggttcatggg
     4501 tttcgttgac atctttcagt aattgataaa agattttgcg ggcttggctt ggatttacga
     4561 tcgacatagg atctcccccc tttttagttt cttgcatcat tatagcgtac ttaattaagt
     4621 acgtaaaaag gcgtgccgaa atgaattgct gagaagttgc gcagtttatg gttctcctga
     4681 tgtatttttt cgactttgca gggtctgatt agttcaaaga taatagcact gcagtgaagt
     4741 aatagttgca ctaattcgct gttttcactg gaaaaagttt tgctgcgctg gaaaccgcag
     4801 gcgtaaaaaa tcaattttcc ccattcgtgg cgcgcttttc caggaatcag accatcaaat
     4861 atgattttca ccctcccctt ctaaattcca acaattgaat tagttcaaaa attggacacc
     4921 ttttccggtt cgaggactgt attaatttat tttttatttg gtctatactc tggactatag
     4981 agattgaaag gggatacaaa aaattatggc aaacaactgg ttggaattag acggaaaaac
     5041 ggttatcgtt acaggaggaa attcagggat cggcttacac atcgcggatc acttgaaaga
     5101 acaaggcgca aacgtcgttg tagctgattt aagtgttgaa acgggaacta gtgcatatgg
     5161 taaactgaat gtgaagacag atgtgacttc catcgagagc atcgaggcaa tggtcgaaaa
     5221 agctgttgag aaatttggaa aaatcgacgt tttggtcaat aatgcaggta tgaacttgcc
     5281 gcgtttattg gtggatgtct acagtgatga aagaaaatac gaaatcgatg aagactcatt
     5341 caacaaaatg acagcagtca atcaaaaagg aatggtattc actacccaag ccgtcgtcag
     5401 aaacatgctg aagaacaaag tggaaggcac gatcatcaat atctcttccg aatccggcat
     5461 ggaaggttcg gtcggccaaa gcatctattc agcaacaaaa ggcgctacga attcttacac
     5521 gcgttcatgg gcaaaagaac tgggtaaatt tggcatcaaa gtcgtcggta tcgcaccggg
     5581 gatcaacgaa aaaacaggat tgacaactcc tgcctacaat gaagcattgg cttacacaag
     5641 aaacataccg gttgagcaat tggacacagg gtatgaaaag agcattccac tgggacgcgt
     5701 cggaaaattg gatgaaatcg gctacttagt ggcattctta tcttccaata aatccgctta
     5761 tatcactgga acaacaatca acattacagg cgggaaatct agaggatagt atgggggcat
     5821 tgagatgtca acggcaaatg atagagagct tttgctgaga agtttacttt caaataaaga
     5881 aaccgatctt caaagtctcg aggaagcgtc gggaaaaaac aaataccaga tcaaaacgat
     5941 catttcggaa ttgaacgatg tctacgaggg gttcttaaaa atccaacaaa aggatgaatc
     6001 caaagtaggc atcgtcagaa aggagggggc gaacatattc aacacacttc attacaatat
     6061 ccatcaagac ttcaataaag tagaatgcag gatcgcgtat ctgttgtacc gcttgatatg
     6121 tacagaggag tacctgaata tcggagacct ctccgaagaa atgagtgtaa gcaggagcac
     6181 ggtgaacaac gacctcaaaa agctgaagca gctttcggag aaatacaatg cgaaagtcgt
     6241 cggaatcccg aacaaaggca tcaccttcca gtgcagcgaa ttcggcaccc gcttggtcct
     6301 gatttacgaa gtattcgagt tgctgcagat gacttttctg ccggaccaag aaatccagga
     6361 tgcgcttgac ggactcgtcc gggactacaa gctggatgag caagcgaaag aactgcttta
     6421 taaggcgttg gctgtaagcg tacacaggtt ggcgcatggg catacgatag attccgaaat
     6481 tccgatgtac aaaaactttg aggcggactc ggcaaatatc atcgcctata cccaactgct
     6541 gcaaaagaaa tttgcagttg cgttgaatga ccatgaaatt gcgtttcttg ctttcccgat
     6601 caacacaagg aactccgcct acgtgaaagg gagcgaaaat tccgaaaacg aagaagttct
     6661 gagacaagtc gtttccagta tgctgaaaac ggtaagggat caagtcatga tcgaaatcga
     6721 tgaagaagcc ttctttgata aagtcaaata tcacttgctt ttcctgataa acaggctggt
     6781 cttccggatt ccgaacacgg atctgtatct ggagcagatc aagctcaaat accctttggc
     6841 cttcgagctg gcgaagattt cgctgtcgga attgaatcgc ctttaccaat tgcgtgcaag
     6901 cgcggacgac atcagctatc tggctgtcta ttacgcactg atgctggacg aaaggcaagc
     6961 gaacgattcc aaagggaaaa gcacaaagga tgtaggcatc atcaccaacc ttggaagagg
     7021 gagttttgag ctgatctgca ggcagatcaa agagatcgtc ggaagtgaga tgaatatcgt
     7081 ccacatcaat accgacagtc tgccgaaaga aaaattgaag cggttccggc tgctcttcac
     7141 aaccgaagag atttccgtgg acaccgaagt ccctgtcatc aagatcgaca gattggtcag
     7201 cccggacgat ttggcccaaa agatctatgc cgtggaaaaa gatcaataca acatcaaata
     7261 cctgacaaac gaggacatca gttatcacct cgtcaatctg aagggtcaga tgggttactt
     7321 cgattatgtg gaagaaatca tgtcctacct ttccagaaga taccgtctgt ctgaagatat
     7381 cctggaccgg ttcgtggaaa aagaagaaag acacacaatg atttatgaga acaaggtagc
     7441 tttcccgcat ttgaccgacc caaacatcaa caagttggtg tttgtcctgg gaaacatcac
     7501 tatggccaac cgcaacgacg cgttttcctt gatgttattt ctagtgacac ccgagaaaat
     7561 ttctgagcag caagagagaa tcttgatgca ggtatatgat tttgtcttta aggtcatcaa
     7621 tgatccaact ctgatcaatg atctgaaaaa agtggatgat gcacaaggac ttattgagat
     7681 gctggagaaa agaggtacga aatagatgaa taatatgctt gcattgggta ttttggttgt
     7741 cggcgctttt ttgatccaaa gcgcactggg cttttttcag atcagacatt tcggcaatga
     7801 atacaacgaa ctgaaggcta acggcagagt tgcgataggc aaacgggcag ggaagctgac
     7861 ttccggaacg atcatcatgt tccaactgga cagcaacggc atgatagtca gaggcaaaaa
     7921 gctgcagggg accacaatct tggccaggtt caaaccatac aataatttca acggcagact
     7981 gatcgaagcc atcaaggcag aggattcgga tgtgctggct gaaatcaaga tcacccgcaa
     8041 aaccatcatg aatgcggtca ctaattaccg gatgttcata aacggagaaa tcattccgga
     8101 gaaagaatct ccctttaagg aattgaccaa aaaaattaca aacattaagt tcggttaaat
     8161 cgtgtcagaa ataatacata taataaggag tgttaaatat ggactttcta gttaaaggtg
     8221 cagaaagctt tatcggatta tttcaattag gtgcaaatac atttatgagc tggatgaccg
     8281 gcatcgttcc aatcgtcttg atgctgatgg tattcatgaa cgtcgtgatc aaactgatcg
     8341 gtgaggaaag aatcaacagc ctcgcacaac gcttctcgaa atattccttg gtcagaaact
     8401 gtatcctacc tttcttggca gccttcatgt tggggaatcc cgcatcattc accctaggaa
     8461 gatttttgcc ggaattctac aaaccaagtt tctatgcggc acttgcacaa tacaaccaca
     8521 cgagtaacgg ccttttccca catatcaatg caggtgaatt gttcgtattc ctgggaatcg
     8581 cgtcaggtgt tgaaacgtta ggattgagca ccacgccatt ggctatccgt tatctgcttg
     8641 tcgggcttgt gatgaatgct gtaggcgcat atgtaaccga cttcacaact gcttatgtct
     8701 gcaaaaaatc aggcatccaa ttgagcaaaa cagcgaaagt tcaataataa cgaccaagaa
     8761 gatgttgcat taaacaaata gaatgcatga gaacgagagg ggtattcatt atgacaaaat
     8821 ataacagcat caaagtcgaa aaaggttccg gcggctgggg cggaccactg atcatcacac
     8881 caacagagca aaaacataaa ttcatctaca tcgtaggcgg cggagaaaaa cctgccatcg
     8941 tcgacagaat cgctgaactt acaggtatgg aagccgtaaa cggattcaaa acatccattc
     9001 cggatgatga aacagccttg gctatcatcg actgcggcgg aacattgaga tgcggcatct
     9061 atcctcaaaa aggcatcccg acaatcaacg tcgttgccac cggcaaatca ggtcctttgg
     9121 ctaaatacat caacgaagac atttacgttt caaatgttgg cttgaatcag atccaagtaa
     9181 ctggcgagac agcaaatatt gagacagtca gcacagcagt tgcagaacca gtagtagaag
     9241 agaaagtcgc ttacactaca gataaaaaat tgacgcaaca aaacgcagaa aaaggcagcg
     9301 tagtggcaaa aatcggtttg gctgcaggta aagggatcgc aatcatgttg caatcagccc
     9361 gtgaagcggt gcagacagtc ttgcagacga tcattccgtt catggccttt gtagcgatgc
     9421 tgatcggtat catccaaggc agcggaatcg gcaatctgtt cgcgaaaatg atgattccat
     9481 tggcaggcaa tgtctggggc ttgatggtca tcggtttcat ctgctccttg ccgatccttt
     9541 ctccgctatt gggacctggt gctgtaatcg cgcaagttat cggaacattg atcggtgttg
     9601 aaatcggcaa aggcaatatc gaaccaagct tagctttacc ggctttgttc gccatcaaca
     9661 cacaaaatgc ttgcgatttc attccagtcg ggttaggctt gcaggaagca gaagccaaaa
     9721 cagtagaagt cggcgtacca tccgttcttt attctcgatt cctgaacggt gtgccccgcg
     9781 tattcgtcgc ttggttagca agcttcggac tatattaaaa aagaagaaaa gcgggacaag
     9841 cccgctttgc ctcgaaagnn nnnnnnnncg tcatcggaag acacctaaac caaatttgag
     9901 aaaagcggaa cgggttcgtt cagccctgaa agaattttag gaaatcgtgc cgactgagca
     9961 tcgcagagcg caataggtac gatttatcta atttccgaag ggcttacccg tgaagctagc
    10021 catcttttgg gaatgcagaa cattttgcaa atgtgtgaag cacctaaggt gatgcacttc
    10081 tgagattgaa tatttataaa ttactatagg cttaaaaact aataatcgga aaaggtgaag
    10141 taaaatgtca atttattcaa cggtagtcac agaaatcgga cctaacgcta aggagttttt
    10201 agctgaggat atgatggtat tgttcggaga cgatgctccg gaagcattgc ggccatattg
    10261 cttcctgatc gaacaaaatg cactggagca ggatattcaa ctaggacacg cgttggttat
    10321 cggcgacgat agttatcgcg tcacagcagt gggtaatgtg gtcaacaaaa acctcaggga
    10381 tctggggcac atcaccatca acttcagcgg agaaacagtc gctgaattgg caggctctct
    10441 gtatgttgaa cacaaaacag tgaacgaaat cgcggaaggc accatcatca aaatcactga
    10501 aactgtgggg tagtagcgta tgtacataga ttcgaacgac atcaaagcaa tcggaagttt
    10561 tttgaaaagc ggatttctga aaggagtcac caccaacccg acgattttgt cgactggttc
    10621 cggcagtcgc ttcaaacaat tacaagattt attggctttg gagccgacgg aactttttgt
    10681 acaattgctg ggtacgactt ctgaagaaat gtttgccgat tatgaagcaa taaagacatt
    10741 tgatgcaaat caccgacttt cgataaagat tcccgttaca caagcaggct tggaaacaat
    10801 caaaaaagtg aaagaggacg atccggaaag agcgatcctg gggacactga tctattctgc
    10861 ggaccagggg atcctggccg ccgcagccgg atgcgattat ctggcaccgt atgtgaacag
    10921 gatgctgaac aatgcgatcg atcccttcaa ggcgatccgt tcgatgcgga cattcatcga
    10981 tgccagaggc tacaagacca aaatcatggc agcgagcttc aaaaattccc aacaagtcat
    11041 cgattctttg gagtcgggcg cgcatactgc cacgatccct ccggatgtcc tgaa
//
LOCUS       DAOMUJ010000011        10830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1470_length_10830_cov_2.362877, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000011 DAOMUJ010000000
VERSION     DAOMUJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_10830_cov_2.362877"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLT73_00715"
     CDS             complement(<1..941)
                     /locus_tag="PLT73_00715"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRM18934.1"
                     /translation="MKKLVNSQQMKKIDAFTIDEIGIPAMVLMENAANEVVAAMEERI
                     SGEDIILVICGSGNNGGDGMAAARILLNHGFQVDVFLIGERDKLSPESRKQLDIIENL
                     EMVIWDDISEINFKGYTVLVDAIFGIGLDRPAEGKYAETIQLMNAFQGYVFAVDIPSG
                     ISADNGQVLGTAVRADVTTTFGIEKIGQLLYPGSLHTGKLIVSDIGFPPSAMDVISSN
                     VLFYEESDLFSLPRRAAYSNKGTYGRVVIIAGSANMSGAAFLAAKAAYRSGAGLVQII
                     TPDENRAILQMQLPEAILTTYQPDGLDQESERLKIIDA"
     gene            1189..1313
                     /locus_tag="PLT73_00720"
                     /pseudo
     CDS             1189..1313
                     /locus_tag="PLT73_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010827875.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     assembly_gap    1314..1323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1324..2078
                     /locus_tag="PLT73_00725"
     CDS             <1324..2078
                     /locus_tag="PLT73_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRM18935.1"
                     /translation="AQVSAAVNAIFHNDRINQVAEYLAFLREAEVDSITLGDPGVVQV
                     MREQDLFIPYRYDAQVMVTSSGQINFWAKRGAVGSVLAREVPFEEMKKLIPGALVPVE
                     VLVYGATCIHQSKRNLLENYFNFIEKEEAVNKERGLFISEPKKVDSHYSIYQDRNGTH
                     IFANNDLDLMPHLGELTAIGVSQWMLDGLFTPGENFVAIAKLFVEAREALAEGNWTEA
                     LAERLDAELHALHPANRELDSGFYSKDPNEVV"
     gene            2115..3353
                     /locus_tag="PLT73_00730"
     CDS             2115..3353
                     /locus_tag="PLT73_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM18936.1"
                     /translation="MRTITKKPEVLAPGGTLEKLKTAIHYGADAVYIGGEAYGLRSRA
                     GNFDYEEMAEAVAFAHSHGSKIYVAANMVTHEGNEEGAGEFFRTIRDIGIDAVLISDP
                     ALMEIAVTDAPGLPIHLSTQASATNYQTLNFWASEGLERVVLAREVSMAEIQEMNEKT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSNRDANRGGCAQSCRWKYGLFDMPLLGQQNPV
                     GAGEEGIEEKFSMSAVDLSMLEHLPDLIESGVDSLKIEGRMKSIHYVSTVVNVYRKAV
                     DAYCADPDHYELKQEWVDELWKVAQRELATGFFYKDPTEDEQLFGQRRKIPRYGFVGQ
                     VMAYDPETQMATIQQRNNFGVGDEIEFYGPGLRHNKLTVEAIWNEHDEAIDRAPNAMM
                     IVKMKVPFAVERFDMIRKKK"
     gene            3682..6405
                     /locus_tag="PLT73_00735"
     CDS             3682..6405
                     /locus_tag="PLT73_00735"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM18937.1"
                     /translation="MENERTTTKTIAAKKNAGYPHNNKPGGDSLAAYQKSLENIFAET
                     GSTKEGLDEQSVKTKQAEHGFNELETKERDSVLKLFVETFKDAMVLVLLAVATIQMVM
                     GHAAESLIIFAVLMINAIVSVIQTRKAEGSLDALKSLSAPMAKVKRAGMRMTVPAREL
                     VIGDIVSLDAGDYVPADGRLFEASALKVDEGMLTGESIPADKEVMDINGAVPVGDRSN
                     MVHSGTLAVYGRGEFIVTAIANDTEIGKVANLLDNALAKQTPLQKNLEHFSKKLGVGI
                     LMLSGLIFGIQFIRMMAEGTTDIGSGVLSAFMFAVAVAVAAIPEALQSIVTIVLSIGT
                     KKMAKKHAIIRKLSAVETLGSTSIIATDKTGTLTQNKMTIVDHYLVNGQSGSFNAHPQ
                     TWSSDEQRLMQVAVLANDANINMDGHEVGDPTEVAMIAFSNKMNQPYDELRKAYPRVA
                     DLPFDSERKLMSTVHTINGEHLLLTKGGPDVVFGRSTKVLIDGEVVPLTNEILENIKE
                     KNEHFSDRALRVLAFAYRPMEAAEALDFDAEHDLILVGLMAMIDPPREEVYGAVAEAK
                     KAGIKTVMITGDHKTTARAIARDIGISGEDDLALTGQELDNLSDRELDAVLERVSVYA
                     RVSPENKIRIVRAWQNKGHVTAMTGDGVNDAPALKQADIGIAMGSGTDVAKEAAAMVL
                     TDDNFVSIVSAVGVGRNVYDNIKKAIAYLFSGNLGAIIAIIAALLMDWVNPFTALQLL
                     FINLVNDSIPAIALGMEKGEPDVMSRKPRDPNEGIFSGDTLVSVIYRGILIGGAVILS
                     QFIGMVYSAEISVAMAFSTLIIARTLQTFPARSNSQTAIGAGFFSNKYVLYAVAFCST
                     LYAATLLPAVRGIFSIPADFGLVHFGIAAGLALTAVALMEATKLIIKRVRN"
     gene            complement(6479..7669)
                     /locus_tag="PLT73_00740"
     CDS             complement(6479..7669)
                     /locus_tag="PLT73_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM18938.1"
                     /translation="MGYFKERLYQQYVILFIFYFMSMAALSSLITVYMRDIGKNGSEI
                     SFILILSNIIALIIQGLIGYSNEQWGTRKTVTIYVLISAVISMGLFFFTGNWLFGLVY
                     GLANAITLGLAPLFDVLAANSPYRFGQIRLWGTIGYAAGQQISGIIYDFLSPKSIFIL
                     YLACYFASIVFLYTLPKPEQPIVSSKQPAEKKEEISGGHTIRNIFQNKLFITYLLLAF
                     IIFGTNTVNTSYLPLLLTDTGAPVAIVSTTLTVALFAEVIAMAFSHRFMDHFTNKQLL
                     LAVLFLSGTEYLINFLSDDFLVLSIATVLTKFFASGTFIMLNLKMVSTLLNRKLIATG
                     TAVVAMLSRNIGTIFFQLMAGPVIDNVGLNYLYLILFVFTVLAAVIALLFKVPAQPKE
                     RLFS"
     gene            7884..8906
                     /locus_tag="PLT73_00745"
     CDS             7884..8906
                     /locus_tag="PLT73_00745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRM18939.1"
                     /translation="MYPQTQKKIAEMLKGNIFPGVSYTFIDMDKTETRMVGSAAILPE
                     RESLREGMLYDVASLTKVIVTTTLLLQLQEEGAINFEDPVQAYLPEFPSAAVTIRHLL
                     THTSDLKGYIKNRDALTADELIAALIALTPGENLGKNVVYTDTGLLLAGFIIEKLTGK
                     SVEKNFEERIKRPLAMTESTFHPTDPMRCVPTQNHPTRGVIRGVVHDPKALSLGGHCG
                     SAGLFAPMKDLIRFSRMMLHFGEWEGQRILREESIRTLLRDWGNVPSQPRSLGWNLIQ
                     EGEDFVLWHTGYTGTFMILDLNQQTAFILLSNRVHLKDHRPEWIAVRDELIAIYRKEA
                     RKETAE"
     gene            9303..10625
                     /locus_tag="PLT73_00750"
     CDS             9303..10625
                     /locus_tag="PLT73_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HRM18940.1"
                     /translation="MAINQKPLLLDVDEKPEVLKGILLSFQHVFAMFGATILVPLILG
                     MPVSVALFASGIGTLIYQVATKAKVPVYLGSSFAYITAMAVAIEQMGGDIKAAQTGVI
                     MVGLVYVLIAGIVKMLGTKWIDKLLPPIVIGPMIMVIGLGLASSAVTNAGFVADGDIR
                     HIIVAIATFLITSFINTKGKGFFKIIPFLIGIIGGYVIALFMGLVDFQPVLDAKWFEL
                     PGFYLPFETPWFNSYDLYFGPETLAIIPVALVTISEHIGDHTVLGKICNRQFLKDPGL
                     SRTLMGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIGLSFFGK
                     FTALISTIPTSVLGGMSILLYGVIASNGLKVLIEEQIDFNHVRNLIIASSMLVLGLGG
                     AVLDIAGVVTLSGTALSAITGIALNLVLPHEEAYNPKHPIHKKEEHKH"
     gene            complement(10720..>10830)
                     /locus_tag="PLT73_00755"
     CDS             complement(10720..>10830)
                     /locus_tag="PLT73_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydrofolate--homocysteine
                     methyltransferase"
                     /protein_id="HRM18941.1"
                     /translation="PQCGFASTEEGNNLTEEQQWAKLALVKQIADEVWGD"
BASE COUNT         2856 a         2472 c         2867 g         2625 t
ORIGIN      
        1 gcgtcgatga ttttcaggcg ttcactctcc tgatccaatc catccggctg gtaagtcgtc
       61 aggatggctt caggcagctg catctgaagg atggcgcggt tctcatccgg tgtgatgatc
      121 tggacaagac ctgcgccgga acggtaggcg gcttttgccg ccaagaaggc agccccgctc
      181 atgtttgcgg atccggcgat gatcacgacc cggccgtaag tgcctttgtt cgaataggcc
      241 gcgcgtctcg gaagcgaaaa aagatcgctc tcttcataga acagcacatt cgaagaaatg
      301 acatccatgg cgcttggcgg gaaaccgatg tcgctgacga tcaattttcc ggtgtgcaaa
      361 gaacctgggt aaagcagctg gccgattttt tcgatcccga aagtggtggt cacatccgcc
      421 cggacagcgg tcccgagcac ttgtccattg tcggctgaga tgccggatgg gatgtcgacg
      481 gcaaagacat atccttggaa cgcgttcatg agttggatcg tctccgcgta ttttccttct
      541 gccggcctgt ccaacccgat gccgaaaatc gcatcgacca agacggtgta gcctttgaaa
      601 ttgatttcgc tgatgtcatc ccagatgacc atttccaggt tttcgatgat atcgagttgt
      661 tttctggact caggcgataa tttgtcacgt tcaccgatca ggaatacatc cacctggaag
      721 ccgtggttca acaaaatgcg ggctgccgcc attccgtcgc cgccgttgtt gcctgaaccg
      781 cagatcacga ggatgatatc ctctcctgat atgcgttctt ccatggctgc gacgacctca
      841 ttcgctgcat tttccatcag caccatggct gggatgccga tttcatcgat cgtaaaggcg
      901 tcgattttct tcatctgttg gctgttgaca agttttttca ttaagtattc ctcctgaacg
      961 ttgatggatg tcattccatt ttcacatttg ctattatacc attcttagtg ctggaaagaa
     1021 tacggggagc ctgatatagc tatatatagg cggaatctaa tgattgtctt aacaagccac
     1081 agcacttttg cacagtaaat ttctaaaaac ttagggaaaa cagtcattca ctttttataa
     1141 gtaatgtgat aatcttatca aggaactcga ataagggagg aatgtaggat gatcgagctg
     1201 attgcaacag ccgagtcagt agcgcaagcc aaggaattgg tggattgcgg tgtagatata
     1261 ctatatatag gagaggatga gtacggcctt cgtttgccgt attccttcac cagnnnnnnn
     1321 nnngcgcgca agtcagtgcc gcggtgaacg cgatcttcca taacgatcgg atcaaccagg
     1381 tggccgaata tctggctttc ctgcgtgaag cggaagtcga cagcatcacg ttgggcgacc
     1441 ccggcgtcgt gcaggtcatg cgggaacagg atttgttcat cccttaccgt tatgacgcac
     1501 aagtcatggt gacttcaagc ggacagatca atttctgggc gaaaagggga gcggtcggtt
     1561 ccgtgctggc ccgcgaagtg cctttcgagg aaatgaagaa actcattccg ggcgctttgg
     1621 ttccggtcga agtattggtg tacggtgcta cttgcatcca ccaatcaaaa cgtaatttgc
     1681 ttgaaaacta cttcaatttc atcgagaaag aggaagcggt gaacaaagag cgcgggttgt
     1741 tcatttccga gccgaaaaaa gtggattccc attattccat ctatcaggac cgcaacggaa
     1801 cgcacatctt cgccaacaat gatcttgatt tgatgcctca tctgggcgaa ctgacggcta
     1861 tcggtgtctc gcaatggatg ttggacgggt tgttcacacc gggagaaaat tttgtcgcaa
     1921 tcgcgaagct gttcgttgag gcgcgcgaag ccttagcgga agggaactgg acggaagcat
     1981 tggcggaacg tttggatgcc gaattgcatg ctttgcatcc cgccaaccgt gagctggata
     2041 gcggattcta ttccaaggat cctaacgaag ttgtctgata ctttgatact gaaagaaaga
     2101 gagtgtacac acacatgcga acaataacaa agaagcccga ggtcttggcc ccgggcggaa
     2161 cattagaaaa attgaagaca gccatccact acggagctga tgccgtctat atcggtggag
     2221 aagcttatgg attgcgcagc cgcgcaggca atttcgacta tgaggaaatg gctgaagcag
     2281 tcgcttttgc acatagccac ggttcgaaga tctatgtcgc cgcgaacatg gtcacccatg
     2341 aaggcaacga ggaaggtgca ggcgaattct tccgcacaat ccgcgatatc ggcatcgatg
     2401 cggtgctgat ttcggatcca gcgctgatgg aaattgcggt gaccgatgca cccggcttgc
     2461 cgatccattt gtcgacacag gcttccgcga ccaattacca gacgctcaat ttttgggcga
     2521 gcgaaggctt ggaacgcgtt gttttggccc gtgaagtatc gatggcagaa atccaggaaa
     2581 tgaatgagaa gaccgatgtt gaaatcgaag ccttcgtcca cggggcgatg tgcatctcct
     2641 attcgggccg ttgtgtcctc tccaaccaca tgtcgaaccg tgatgccaac cgcggaggct
     2701 gcgcgcagtc ttgccgttgg aaatatggct tgttcgatat gccgttgttg ggacaacaga
     2761 accctgtagg tgccggcgaa gaaggcatcg aagagaaatt ctccatgagt gccgtcgatc
     2821 tgtcgatgct ggaacacctg cccgacctga ttgagagcgg cgtagacagc ctgaagatag
     2881 aagggcgcat gaaatcaatc cactacgtgt ctacggtagt caatgtttac cgcaaagctg
     2941 tggatgctta ttgcgccgat ccagatcatt acgaattgaa gcaggaatgg gtggatgaat
     3001 tgtggaaagt cgcccaacgc gaattggcga ccggcttctt ctacaaagat cctaccgagg
     3061 atgaacagct cttcggccaa cgccgcaaaa ttccgcgcta cgggttcgtc ggccaagtga
     3121 tggcatatga tccggaaacg cagatggcca caattcagca acggaacaat ttcggtgtcg
     3181 gggacgaaat cgaattttac ggtcccggcc tgcgccacaa taagctgact gtggaagcca
     3241 tctggaacga acacgatgaa gcgatcgacc gtgcacccaa tgccatgatg atcgtgaaga
     3301 tgaaagttcc tttcgcggtt gaacgtttcg atatgatccg caaaaagaaa tagaaaaagt
     3361 gtaagcttaa gacttgcttt tttcgggctt accgtttata ttgattaaga actgaataga
     3421 agtttttccg taagggagta actagcagct ttgctgtggc aatgccaaca ataagtagat
     3481 ccgagagggt cttctggtgt tgccttaaat agtgagactt atgcctaacg tttcttgaga
     3541 gagacgttag tgcataagtc tcttttttat tagttatttg cggaaaacaa ggagggaagc
     3601 gcggcgaaaa cttgaagcag gaaacagata agggagcaac ggctggttaa aaatagatat
     3661 ccaataacag ggggatttca gatggaaaac gaaagaacaa ccacgaaaac gatagcggca
     3721 aaaaagaatg cgggctaccc gcacaataac aaacctggag gggattcctt ggcggcatac
     3781 cagaaaagct tggagaatat ttttgcggaa acgggcagca ctaaagaagg tctcgatgaa
     3841 cagtccgtca aaacgaagca agcggagcac gggttcaacg aattggaaac gaaggaacga
     3901 gattcggtcc tgaagttgtt tgttgagacg ttcaaggatg caatggttct ggtgttgctg
     3961 gctgttgcta ccattcagat ggtgatgggg catgcagcag aatcattgat catctttgca
     4021 gttctgatga tcaatgcgat tgtcagcgtc atccagacaa ggaaagcaga aggctcgctg
     4081 gatgcattga aaagtttgtc cgctccgatg gcaaaagtta aaagggccgg tatgaggatg
     4141 accgttcctg ctagggaatt ggtcatcgga gatatcgtca gcctggatgc tggtgattat
     4201 gtcccggcag acggtcgact gtttgaagcg agtgcgctca aggtggatga agggatgctg
     4261 accggggagt ccatccccgc cgataaagaa gtgatggata tcaacggagc agttccggtg
     4321 ggcgatcgct cgaatatggt ccatagcgga acgctggctg tgtacggccg gggagaattc
     4381 atcgtcaccg ccatcgccaa cgacaccgaa atcggtaagg tggcgaactt attggataat
     4441 gcgttggcaa agcaaacgcc gctgcaaaag aatctggagc atttcagtaa aaaattgggt
     4501 gtgggcatcc tgatgctttc cggacttatt ttcggaatcc agttcatccg tatgatggcg
     4561 gagggaacga cggatatcgg gtccggcgta ctgagcgcct tcatgttcgc tgtcgccgtc
     4621 gcggttgcag cgattccgga agccctgcag tcgatcgtga cgattgtcct ttcgattggg
     4681 acgaaaaaga tggccaagaa acatgcgatc atccgcaaat tgtctgcagt agaaacgttg
     4741 ggatcgacca gcatcatagc caccgataag acaggaactt tgacccagaa taagatgaca
     4801 atcgtcgacc attatttggt gaacggccaa tccggtagtt tcaatgctca tccgcagacc
     4861 tggtcatccg atgagcagcg cttgatgcag gtcgccgtct tggctaatga tgccaacatc
     4921 aatatggacg gacacgaggt gggggacccc acggaagtag ccatgatcgc cttcagcaat
     4981 aaaatgaatc agccatacga tgagctaagg aaagcttatc cgcgtgtggc ggacttgcct
     5041 ttcgattcgg aacgcaaact gatgtcgacg gtccacacaa tcaacgggga gcatctgttg
     5101 ctgacgaaag gcggcccgga tgtggttttc gggcgcagta ccaaagtttt gattgatgga
     5161 gaagtggtgc cgttgacgaa tgaaatcctt gaaaacatca aggaaaagaa tgaacatttt
     5221 tcggatcgtg ccctccgcgt attggctttc gcctatcggc cgatggaggc agcagaggcg
     5281 ctcgatttcg atgcggagca tgatctgatt ctggtcgggc ttatggcgat gattgatcca
     5341 ccgcgcgaag aggtttatgg cgctgttgcg gaagccaaga aagcaggcat caaaacggtg
     5401 atgatcaccg gcgaccataa aacgacagcg cgcgcgattg cccgcgatat cggcatttcg
     5461 ggagaggacg accttgcttt aaccggacag gagctggaca acctaagtga tcgggaattg
     5521 gatgctgttt tggaacgtgt atccgtctat gctcgggtat ctcctgagaa caaaatccgg
     5581 attgtccgcg catggcagaa taaaggtcat gtcactgcga tgacgggtga cggggtgaac
     5641 gatgctccgg ctttgaagca agcagacatc ggcatcgcca tgggcagcgg aacggatgtc
     5701 gccaaagagg cggcagcgat ggtgctgacc gacgataact tcgtttccat cgtcagcgcg
     5761 gtcggagtag gccgcaacgt ctatgacaac atcaagaaag ccatcgccta tctcttctcg
     5821 ggcaacttgg gggccatcat cgccatcatc gcagcgctgc tgatggactg ggtaaatcca
     5881 ttcacagccc tgcaactgct gttcatcaac ttggtcaatg attcgattcc cgcgattgca
     5941 ttgggtatgg agaagggcga gcctgatgtg atgagtcgca aaccaaggga tccaaacgag
     6001 ggcattttct ccggagatac gctcgtttct gtcatctacc gagggatact gatcggggga
     6061 gccgtcatcc tttcgcaatt catcggtatg gtttattctg ccgaaattag tgtggcgatg
     6121 gccttctcaa ctttgatcat cgcgcgtact ttacagacgt ttccagcgcg ttctaattcc
     6181 cagaccgcta tcggagcggg tttcttttcg aataaatatg tcctttatgc tgtagctttc
     6241 tgcagcacat tgtatgcggc gactttgttg ccagcggtgc gcggcatctt ctcgataccg
     6301 gccgacttcg gcttggtgca tttcgggata gcagcaggac tggccttgac cgccgtcgcg
     6361 ttgatggaag cgacgaaact gatcatcaaa cgagtcagaa actgatgaat tcctgaaatt
     6421 aaaaaaaacg gccggggaaa ttggattccc tggtcgtttt ttgcgattaa agctatcatc
     6481 aagaaaaaag gcgttctttc ggctgagccg gtactttgaa cagcaaggca atcaccgcag
     6541 cgagaaccgt aaagacgaaa aggatcaagt agagataatt gagccctaca ttatcgatta
     6601 ccggacccgc catcaattgg aagaagatgg tgccgatgtt cctggaaagc atggcgacga
     6661 ctgcagttcc tgtagcgatc agtttccggt tcagcaaggt agagaccatc ttgaggttca
     6721 gcatgatgaa ggttccggaa gcgaaaaact tggtcaatac ggtcgctatc gaaagcacta
     6781 aaaagtcatc cgacaggaaa ttgatcagat attccgttcc tgaaaggaac aaaacggcca
     6841 gaagcagctg tttattcgtg aaatggtcca tgaagcggtg tgaaaaagcc atcgcgatga
     6901 cttccgcaaa cagggctacg gtcaaggtcg tgctgacaat tgcaaccggg gctcccgtat
     6961 cggtcaaaag cagcggaaga taactggtgt tgaccgtgtt cgtgccgaaa atgatgaacg
     7021 ccaaaagcag ataagtgatg aaaagtttgt tctggaatat atttctgatt gtatgccccc
     7081 cggaaatctc ttctttcttc tcggctggct gttttgaaga gacaattggt tgctccggtt
     7141 ttggcaacgt ataaaggaac acgatgcttg cgaagtagca ggcgagatag agaataaaaa
     7201 tggattttgg agacagaaaa tcatagatga tccctgaaat ttgttgcccg gccgcatagc
     7261 cgattgttcc ccagaggcga atttggccga atctgtaagg actgttggcc gcgagcacat
     7321 caaacagagg tgcgagtccc aaggtgatgg cgttcgccaa tccgtaaacg agcccaaaga
     7381 gccagttgcc tgtgaagaaa aagagtccca tgctgatgac ggctgagatc aggacgtaga
     7441 tggtcaccgt tttacgcgtt ccccattgct cgtttgaata accgatcagt ccttggatga
     7501 tgagtgcgat tatgtttgaa aggatcagga tgaatgaaat ttccgacccg ttttttccta
     7561 tgtcgcgcat gtagacggtg atcaatgatg acaaagccgc catggacata aagtagaaga
     7621 tgaataatat cacatattgc tgatacaaac gctccttaaa ataccccatc gaattacatt
     7681 cccccaataa gttcactagt atacaatatt ctaacacatg atccggtgat aaagctgttt
     7741 ctttcttaat ttgaaaagtg cttcgcaaat ctcacaccaa ttctccataa aattgtaaaa
     7801 aacgttcgaa atcggccgtg catcaagcta gggatgcgct ataatgatag acaacgatac
     7861 taacggaaag gtgatgtctg ctgatgtatc cacaaaccca aaagaaaatc gctgaaatgc
     7921 tgaaaggcaa tattttccca ggagtgagct acaccttcat cgacatggac aaaacagaaa
     7981 cgcgaatggt cggatcggcg gcgatcctgc ctgaacggga atccttacgg gaaggcatgc
     8041 tctatgatgt ggcatcttta acaaaagtga tcgtcacgac gacgctgctg ctccagctac
     8101 aagaagaagg ggcaatcaac ttcgaagatc cggtgcaggc gtatctgccc gagttccctt
     8161 ctgctgcggt gacgatccgc catctgctga cgcatacttc agatctgaaa ggctatatca
     8221 aaaaccgcga tgcgttgact gccgatgaac tcattgccgc cttgatcgcc cttacgccag
     8281 gagagaacct aggcaaaaat gtggtctata ccgataccgg attgctcctt gcgggcttca
     8341 tcatcgagaa gctgaccgga aaatcggtgg aaaaaaattt tgaagaacga atcaaacgtc
     8401 ctcttgccat gacggaaagt accttccatc cgaccgatcc gatgcgctgt gttcctacgc
     8461 agaaccaccc gacaagaggc gtaatccgtg gcgttgtgca tgaccccaaa gcgctgtctt
     8521 tgggaggaca ttgcgggagt gccggcttgt tcgcaccaat gaaagatttg atccgcttct
     8581 cccggatgat gctgcatttt ggggaatggg aaggccaacg gattttgcgt gaagagagta
     8641 tccgtacatt gctgagggat tgggggaatg tcccgagcca gccacgctct ttgggatgga
     8701 atctgattca ggaaggggaa gattttgtat tgtggcacac cggttatacc ggaacgttta
     8761 tgatactgga tctgaaccag caaacagcgt tcatactgct ttcgaaccgg gtccatctca
     8821 aagaccacag acccgagtgg atcgcggtcc gcgacgagct gatcgccata tataggaagg
     8881 aagcccggaa ggaaacagca gaatagcaaa aaaacgccac ttttaggatg gcgttttttt
     8941 gctgtccttg gatggacaaa gttctttaga aagtgggcgg cgaagttgca gcttcaccga
     9001 tccggttcgg agatccaaag aaaaagagta gtttctctca gtaactaaag agtatacagt
     9061 gatactctga atatagtatg acagctgatt aacattttgg atagtatcaa atgagaaaat
     9121 tcacatgaaa actgctattg aaaattccac tgacaattga taagatagtg aaagcaaagt
     9181 cctttaatat gattcagaga gatcatgaag ggtaacaaat cacatacatt tgatacctta
     9241 ggtccttttg caaataaacg tcaaaagggt atttttttgc ccaaaaacta ggaggaaaag
     9301 gtatggcaat caaccaaaaa ccgttattat tggacgtgga cgaaaaaccg gaagttctga
     9361 aaggcattct gttgagtttt caacacgttt ttgcaatgtt cggagcaacg atattagttc
     9421 cgttaatttt agggatgccc gtctcggtcg ctttgttcgc gagcggaatc ggcaccctga
     9481 tttatcaggt ggcaacaaaa gcgaaagttc ccgtctatct gggatcttcg ttcgcctaca
     9541 ttacagcaat ggctgtggcc attgaacaaa tgggcgggga catcaaggcc gcacagacag
     9601 gcgtcatcat ggtggggctt gtctacgtcc tgatcgctgg aattgtcaaa atgctgggta
     9661 cgaaatggat cgacaaattg ctgccgccga tcgtcatcgg accgatgatc atggtcatcg
     9721 gattgggact ggcttcgtct gcagtaacca atgccggctt cgtggcggat ggggatatcc
     9781 gtcatatcat tgtggcgatt gcaactttcc tgatcacttc gttcatcaac acgaaaggga
     9841 aaggcttttt caaaatcata cctttcctga tcggaatcat cggaggatac gtcatcgcac
     9901 ttttcatggg tctggtcgat ttccaacctg tattggatgc gaaatggttc gaactgccgg
     9961 gattctatct accgtttgaa acaccttggt tcaattctta cgatctgtat ttcggaccgg
    10021 aaacattggc catcatccca gtcgcattgg ttacgatttc tgaacatatc ggtgaccaca
    10081 ctgttttggg caaaatctgt aaccgtcaat tcctgaaaga tccgggtctt tcccggacgc
    10141 ttatgggcga tggtgtagcg actgcggttt cagctttcat cggtggaccg gcgaatacga
    10201 cttacggtga aaataccggc gtcatcggga tgacgcgcat cgcatccgtt tccgtcatcc
    10261 gtaacgctgc cttcatcgca atcggtttga gcttcttcgg aaaattcact gcgctgattt
    10321 caaccatccc aacatctgta ttgggcggga tgtccattct gttgtatggt gtcatcgcca
    10381 gtaatggtct gaaagtattg atcgaagaac aaatcgattt caaccacgtc cgcaacctga
    10441 tcatcgccag ctcgatgcta gtgctgggac tgggcggcgc ggttctggat atcgcaggcg
    10501 tggttacctt gtcaggtaca gcattgagcg ctattaccgg tattgcgttg aacttggttc
    10561 tgccgcatga agaagcgtat aatccaaaac atccgattca taaaaaagaa gagcacaaac
    10621 attaatgcaa aaaaggcatc gccgctcaat tacagagcgg cgatgccttt tttctattgg
    10681 gatttttggg tgcttagacc aaaaaaacta tgcgaactat cagtcgcccc agacttcgtc
    10741 agcgatctgt ttgaccaaag ccaatttcgc ccactgttgc tcttccgtca gattgtttcc
    10801 ttcttcagtc gaagcgaagc cgcattgcgg
//
LOCUS       DAOMUJ010000012        10544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1519_length_10544_cov_2.875012, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000012 DAOMUJ010000000
VERSION     DAOMUJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_10544_cov_2.875012"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1170)
                     /locus_tag="PLT73_00760"
     CDS             complement(187..1170)
                     /locus_tag="PLT73_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM18942.1"
                     /translation="MHKNKLVVVGVGHVGSYVLADAMKLRLFAEIATIDILKDVAHGE
                     ALDQAHATALTYMSNVDVHAGDYADCADADVIIIAAGPSVLKDENDPNAIPDRASLTG
                     KNAAVIREVMTEIVKYTKEAIIILITNPLDTVTYIAASEFDYPEGRIFGTGTMLDSAR
                     LRKVISQQYGVDPKSVTGYMMGEHGFTAFPVVSRLAINGIRYEEFGTYFPEVETLEPE
                     AIGAAVVKSAYDVLNGKGWTNAGVAQSAITLAQAVLLDEKSVHPVCTILRGQYGHDGD
                     VALSMPCLIGRNGVEKQFGVALNEWETAKLNASIDYIQLAMKDAKTGPFKQ"
     gene            1329..1985
                     /locus_tag="PLT73_00765"
     CDS             1329..1985
                     /locus_tag="PLT73_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM18943.1"
                     /translation="MEKFVEALKQKIRSKEGLSPKMQDILDACVVLFSTKGFSNTSTK
                     DIAKAANVAEGTIYKHFGTKENLLYATIFPILHDIISTEALAQVQQFRSSAENAPFER
                     FVAFIVRKDVMMPEEHFQSGKIFLTEMMYDKQRCQEFITMIPKDLIEGIYAILDGYKE
                     KNEIVDWPNPVIWQFIMSILFGNHLTHYVLFTDTKRDKEAEITYLTQQIVKGLSPDKQ
                     "
     gene            2091..4112
                     /locus_tag="PLT73_00770"
     CDS             2091..4112
                     /locus_tag="PLT73_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM18944.1"
                     /translation="MAFVELKNLNKYYPISGSEDFQALKDVNLSLDKGELVSIVGESG
                     SGKSTLMNLLGGLDSQFSGEITIDGQNMSTFKEKDFVNYHKEKIGFVFQSFNLVSHLS
                     VLDNVTLAMTLSNVDKKTRVARAREVLKQLGLDDQYKKKPSQLSGGQKQRVAIARALV
                     NDPDIIIADEPTGALDSQTTDQVLDIIQDIAKTGKLVIMVTHSEKVAARSTRVVTIDD
                     GKIIGDIHQGEIALHDNAFELQQKSKSKNLTFFAAIRLALLNMKEKLARNILIALGGS
                     IGIMSIIVMLSLGEGVNDYLTETMNANVNPLVSEVRMPIVMETNNSENTDSSMMIMTP
                     AGAEIPAGSAPSGPPAGVTQSDPFGEEDLEKLRNIPHVAEVNLAYSSLTIGSDNVIYE
                     GEKYAFMNFGTTSGMTDSNILYGAFPEAGEILITEGVANQMAGTAEEAIGKEVTVDVT
                     IDGESLEKNYVISGIYSAGQAIGAFESVYMSAESFETLTAENNLEVEPNVVYLVSDDP
                     ENTQAIKDEVAALGYRGSSADALASTFTQMLDIFTYILSGVAGISLFVSAIMILTVLY
                     ISVVERTQEIGVIKAIGGRKKDIRRIFVSESFLIGLFSGILGVGIAYLITVVGNIVVE
                     NLFDTAILNMTPAFAVFGIVTSVIISMVAGLMPAQKAARLDPVEALRHD"
     gene            complement(4695..6098)
                     /locus_tag="PLT73_00775"
     CDS             complement(4695..6098)
                     /locus_tag="PLT73_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18945.1"
                     /translation="MNGDYGLFFEYYYAIYDYVLTAKTTVCERTLHDFLHTQQYYRLS
                     HEAQRFAKRIAEKDIAVDRLLLSGPEAHGWYLFYYKQNFPTGYPFSHVNLEFMEGEYE
                     LKEFSPIIGPLGHQPLHVGILRKRMGEDYLIFPYFKNQTKISHLILRIIVNKLMTEKK
                     YTVRKSIELVQLFEAEAIGSDWEPAKIKAQMEEVLRQQLHFRVNDSDIKKLWLQMIFI
                     NFRYEKDEASYIQWECHAYADTVRKLENFAAVLDGKPDIHLTAEEGALLKWVRFVVFR
                     SRGMHGKAMEEAHKLLEREELLIGASRQPLYSKLDLVPINDTYKDLVVRKLSIDFRDI
                     LVSYLLDSYIANQKYEAAFFLLQQSDPYFKHFIPRYYRSANQNEWLSGEGEISTEQLF
                     REQFQRVRIVLGDHWVDALHKEIEEEAKAGLTLKKDAFDWLLRYLYNVMSIFHEMQEN
                     ELADQMNAFYAQIRLAE"
     assembly_gap    6536..6545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7118..7930
                     /locus_tag="PLT73_00780"
     CDS             7118..7930
                     /locus_tag="PLT73_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM18946.1"
                     /translation="MYTMSNIKNLSLKIVLAEKNTITSAQPTNYQKFPYVKLFYVTTG
                     DVSLVMKETTEKLSKDQLILLNPNVEYYWDLTGDKAAEIIEVGIHGLEFSKIGDAESD
                     FSYYRHNDGEKETATFLNLLLDEMSTRSQGYEEVSKRFVEIILIHLLRLEEFSIRNSL
                     NKKNHKEIQTVKNYMKVNYHKNITLDDLVDLVHINKFYLIRIFKQEVGMSPIDYLIHI
                     RIDEAQKMLRNTNIAIADIAHLVGFQSPSHFSKTFRELSNCTPSQYRRQGNQ"
     gene            complement(7960..8526)
                     /locus_tag="PLT73_00785"
     CDS             complement(7960..8526)
                     /locus_tag="PLT73_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="In Listeria monocytogenes this protein binds a
                     specific sites on DNA, influencing the topology and
                     transcription; regulates flaA, proU and ompC; is
                     osmoregulated; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HRM18947.1"
                     /translation="MAIMKDSGSGKAMKRIVVIGCSGSGKSTFSRKLKQVTGNPLHHL
                     DKLYWLPDWNLRAAEEQDRIQKERIKDEKWIIDGNYQRSLPIRLERADTVFFFDYPRS
                     LCLYRALKRIVLNRKRTRPDMGEGCPERFDWEFMKFIWNFNKNNRPGLIALLDQAPAG
                     IEIQHFRKPREARDYLRALEEQKEEEVA"
     gene            8664..9233
                     /locus_tag="PLT73_00790"
     CDS             8664..9233
                     /locus_tag="PLT73_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM18948.1"
                     /translation="MIRKAIKEDCPELYDLIHEILVDMELPLLTLLEPDVLKAAVVRA
                     MQEDVYRFSYRNGLVYEENGEILGCCFGYPGASEPIIDAALQTAFDKMYITAARLFPD
                     SETFAGEWYLDSIVTKQSARGKGVAYQLLQALPTLASAAGETVIGLNCEKDNLQAKKL
                     YEKVGFRTVSERTLSGHVYDHMQWDFTYN"
     gene            complement(9285..>10544)
                     /locus_tag="PLT73_00795"
     CDS             complement(9285..>10544)
                     /locus_tag="PLT73_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM18949.1"
                     /translation="LAQNGATGTTGRNNVNGYNAYITAIQDIAMGEVWIVDQSAETIS
                     VGTGKKEITYGELPGAALALVDEVFEGDVAFSEGFSPLLEDTSITVGAPVLSAEGEVI
                     AAVLLHDYIANMQTAVRSGFSTLGISLALALIAAVGIAVLLAKRFIQPLKKMEETTQQ
                     LADNHYEAHTGIIQNDEMGSLAANIDILADKLAEAAQESAKLDKMRKDFITSISHELR
                     TPVTVIRGSLEALNDHVVEDPAKIDEYHQQMLTESIHLERMINDLLELSRLQNPDYQM
                     EMSSLNFVDVVENAIRSVRYIAREKGITLTFSKEPLAFMLMGDYTRLRQMLLAVMENA
                     VKFSAEKSEIRITLTGAGEGCRLAITDNGPGIPEKDQPYIFEKFFKSNEESNKQGTGL
                     GLAVAKQIADRHGIGRKHPGKRLHLSI"
BASE COUNT         2860 a         2679 c         2289 g         2706 t
ORIGIN      
        1 ttccgagacc ttcccccttc cagcctctat cctttgcaac cacattgctt ctattgttcc
       61 cgatgaaatc atccagtttg cttatcttaa ttatggatat ccaagcgcac ctttgtcaaa
      121 ttttgcggtg cctttaaggg cgcgcaccaa aatacaacta cctctgcgct agccagctcc
      181 tcattcttat tgtttgaacg gtcccgtctt cgcatccttc atcgccaatt ggatataatc
      241 gatggaagcg ttgagtttgg ctgtttccca ctcatttaaa gcaaccccga attgtttttc
      301 cacgccgttg cggccgatca ggcaaggcat gctcaaggcc acatcgccat catgaccata
      361 ctgtccgcgc agaatcgtac agacgggatg gacgctcttt tcatccaaca ggaccgcttg
      421 cgccaaggtg atggcggatt gcgcaacccc ggcattcgtc cagcctttcc cgttcaagac
      481 atcgtaggct gacttcacga ccgccgctcc gatcgcttct ggttccaacg tctcgacttc
      541 cgggaaataa gttccgaact cctcatagcg aatcccgttt atagccaacc gactcaccac
      601 cgggaaggct gtgaagccgt gttcgcccat catatagccg gtcacgctct taggatcgac
      661 accgtactgt tgcgaaatca ctttgcgcag acgggccgag tccagcatcg tgccggtccc
      721 gaaaatccgg ccttccggat agtcgaattc gctggccgcg atataagtaa ccgtgtccaa
      781 tggatttgtg atcagaatga tgatggcttc ttttgtatat tttacgattt cagtcatcac
      841 ttcacggatg accgcagcat tttttccggt caaagaagcg cgatccggga tggcattcgg
      901 atcgttttca tccttcagga cgctcggccc ggcagcgatg atgatcacgt ccgcatccgc
      961 gcaatcggca taatccccgg cgtgtacgtc cacattggac atataggtca acgcggtcgc
     1021 atgcgcctga tccaaggctt ccccatgagc aacatccttc aaaatatcga ttgtcgcgat
     1081 ttccgcaaat aacctcaatt tcatcgcatc cgccaatacg taggaaccta catgtcccac
     1141 acccaccaca acaagtttat ttttatgcat ctttctccct ccagttctca tcatactttc
     1201 atcttagcac cttttcggcg atttatcaca agtatttcgc gaaggaggct gaatcattgt
     1261 gccggcttgc aataatcggc cggtccgagt actatggaag caacatcacc aagaggagca
     1321 tacgttaaat ggaaaagttt gtggaagctt taaaacaaaa aattcgtagt aaagagggct
     1381 tgtcgcccaa gatgcaggat attctggatg cttgcgtggt actattctcg acgaaaggat
     1441 tttccaacac cagcacaaag gacatcgcca aagccgccaa tgttgctgag ggaaccatct
     1501 acaaacactt cgggacgaag gaaaacttac tttacgctac gattttcccg attctgcacg
     1561 acatcatctc tacggaagcg ctcgctcaag tgcaacagtt ccgcagttct gcagagaacg
     1621 cgccgttcga gcggttcgtg gcattcatcg tgcgcaagga tgttatgatg ccggaagagc
     1681 attttcaatc cggtaaaatt tttcttaccg aaatgatgta cgacaaacag cgctgccaag
     1741 aattcatcac catgattcca aaggacttaa ttgaaggcat ctatgcgatc ctcgacggct
     1801 ataaagagaa aaatgagatc gtcgattggc cgaatcccgt catttggcaa tttatcatga
     1861 gcatcctgtt cggtaatcac ctgacccatt acgtcctttt cacggataca aaacgcgaca
     1921 aagaagccga aatcacttat ttgacccaac agatcgtcaa aggcctcagc cccgataagc
     1981 aatagcattg acagcccttt acagaatcgc tatacttgac taatcgtacg gtgagtaaat
     2041 actcactcat cggcatttct ttcaaaataa taaggaggca tcccctaact atggcttttg
     2101 tcgaactaaa aaatctgaat aaatattacc ctatcagcgg ctcggaagat ttccaggcac
     2161 ttaaggacgt caacctttcc ttggacaaag gcgaattggt ctcaatcgtc ggtgaatccg
     2221 gcagcggtaa atcaaccttg atgaatctgt tgggcggtct cgacagccag ttctcaggcg
     2281 aaataaccat tgacggccaa aacatgagta cgttcaagga aaaagatttc gtgaattacc
     2341 acaaagagaa gatcggcttc gttttccaga gtttcaatct tgtttcccac ctcagcgtgc
     2401 tggataacgt cacgttggcg atgacgcttt cgaacgtcga taaaaaaacg cgggtcgcaa
     2461 gggcgagaga agtcctgaag caacttggcc ttgacgacca atacaaaaag aaacccagcc
     2521 agttatccgg tggacaaaag caacgtgtgg ccatcgcccg tgcgttggtc aacgacccgg
     2581 acatcatcat tgccgatgaa cctaccggag ctttggactc acaaacgact gatcaggtgc
     2641 tggacatcat ccaagacatc gccaaaaccg gtaagttggt catcatggtc acccactcgg
     2701 agaaagtagc ggcccgctcc acgcgcgtcg tcacaattga tgacggaaaa atcatcgggg
     2761 atatccatca aggcgagatc gccttgcacg acaatgcatt tgaactgcag caaaaaagta
     2821 aaagcaaaaa tctcacgttt tttgcagcaa tccgattggc tcttcttaat atgaaggaaa
     2881 aactggcgag gaatatcctg attgctcttg gcggcagcat cggcatcatg agcatcatcg
     2941 tgatgctctc ccttggcgaa ggcgtcaatg actacctgac cgaaacgatg aacgccaacg
     3001 tcaatccgct ggtaagtgaa gtgcggatgc ccatcgtgat ggaaaccaac aacagcgaaa
     3061 acactgactc ctctatgatg atcatgactc ccgccggtgc agagatacct gccggatccg
     3121 cgccatcagg tcctcctgcc ggggtaacgc agtctgaccc gtttggagaa gaagatctgg
     3181 aaaaactacg gaatattccg catgtggcag aagtcaatct ggcctactct tcgcttacga
     3241 tcggcagcga taacgtcatc tatgaaggcg aaaaatatgc cttcatgaac tttgggacaa
     3301 cctccggcat gaccgattcc aacatcctct atggtgcttt ccctgaagct ggcgaaatcc
     3361 tgatcacaga aggtgtcgcc aatcagatgg ctggcactgc cgaagaagcc atcggaaaag
     3421 aagtgaccgt ggatgttact atagacggcg aatcgctgga aaaaaattat gtcatcagcg
     3481 gcatctattc agccggccaa gctatcggtg catttgaatc cgtctatatg tcggcggaat
     3541 cattcgaaac cttgactgcg gaaaacaatc tggaagtaga accgaacgtt gtctacttgg
     3601 tatctgatga tccggaaaat acccaagcca tcaaagatga agtggccgcc ctaggttacc
     3661 gtggttcatc tgcggatgcc ctggcttcca cgttcaccca aatgctggat atcttcactt
     3721 acatcctttc cggggttgcc ggtatctcct tgttcgtgtc cgccatcatg attttgacgg
     3781 tactctacat cagcgtagtg gaacggacac aagaaatcgg tgtcatcaaa gcaatcggtg
     3841 gccgcaagaa agatatccgc cgcatcttcg tttctgagtc cttcctgatc ggtctgttca
     3901 gcggcattct gggtgttggt atcgcctacc tcatcaccgt cgtcggaaat attgttgtgg
     3961 agaacctgtt tgatactgcc atcctgaata tgacaccggc atttgcggtg ttcggcatcg
     4021 tcaccagcgt gatcatcagc atggtagccg gcctgatgcc agcccaaaaa gcagcccgcc
     4081 tagacccagt agaagcccta agacacgatt aaaaaaaaca ggatgtgatg aatcctgagc
     4141 tcagagtttg agcactaacg aggttcagga ccaagcagcc cgcttttggc tgagtgggcc
     4201 tgaacagcgt taggcggaga actctaggat tcagaactca actaccagga tgtgatgatt
     4261 cgcgtttaga tacccggacg ttggagtacc ccacgaagtg aacctctttt gttcactccg
     4321 cggggtgcga aacggtagtg gtatctgcga atcagaactc aactaccagg atacgatgaa
     4381 tcccgtttag aaatcgagca ctaagaggaa atacccaccg cgacccggtt ttggtcgagg
     4441 tgggtatttc agcttaggcg gagagttctg ggattcagag ttcaactacc aggatgtgat
     4501 gaatcctaag ctcagagttt gagcactaac gaggttcagg accaagcagc ccgcttttgg
     4561 ctgagtgggc ctgaacagcg ttaggcggag aactctagga ttcagaactc aactaccagg
     4621 atgtgatgaa tcccgtttag aaatcgagcc caaaaagaag cagtccgacc ttcaagtcgg
     4681 ctgcttcttt ttatttattc tgctaggcgg atttgcgcat aaaaggcatt catctgatcg
     4741 gccaattcat tttcctgcat ctcatggaag atgctcatca cgttgtacag gtaacgcaac
     4801 aaccagtcaa aagcatcctt cttcaaagta agcccggctt tcgcttcttc ctctatctct
     4861 ttatgcaacg catcgaccca atgatctccc agaacaatgc gtacccgttg gaactgttct
     4921 cggaaaagct gctccgtcga aatttcacct tctcccgaca accactcatt ctggtttgca
     4981 ctacggtaat accggggtat aaagtgcttg aaataagggt cggattgctg cagcaggaaa
     5041 aaagcagctt catatttctg attggctatg tagctgtcca ataaatagga aaccaaaata
     5101 tcccggaaat cgatcgatag ttttcggaca accaagtcct tataagtatc attgataggc
     5161 acaaggtcca acttgctgta taacggctgc ctgcttgcac caatcaacaa ttcttcacgt
     5221 tccagcagtt tatgcgcttc ttccattgcc ttaccatgca tgccacggga gcggaaaaca
     5281 acgaaacgga cccacttcag caacgcacct tcttcggcag tcagatggat atccggcttt
     5341 ccgtccaaaa ccgccgcaaa attctccaat tttcggacag tatccgcgta agcatgacac
     5401 tcccactgga tataactcgc ctcatctttt tcatagcgga aattaatgaa aatcatctgc
     5461 agccatagtt ttttgatgtc agaatcattg acgcgaaaat gcaactgttg ccgcagcact
     5521 tcttccatct gcgccttgat tttagcaggc tcccaatccg atccgattgc ctcagcttca
     5581 aataattgaa cgagttcaat cgacttcctg acagtatatt ttttttctgt cattaattta
     5641 tttactataa ttctgagaat gaggtgcgat atcttcgttt gattcttgaa gtaggggaaa
     5701 ataaggtagt cttcacccat tctttttcgt aaaatcccga catgcaaagg ctggtgacca
     5761 agagggccga ttatcggtga aaattccttc agttcatact ccccctccat gaattccaga
     5821 ttcacatgac tgaaaggata tcccgtagga aaattctgct tgtaatagaa cagataccac
     5881 ccatgcgcct ctggtccgct cagcaacagc cgatccacag cgatgtcctt ttcggcaatc
     5941 cgtttcgcaa aacgttgcgc ttcatgggat aatcgataat attgttgcgt atgcaaaaaa
     6001 tcatgcagcg tacgctcaca cactgtggtt ttggcagtca gtacatagtc ataaatcgcg
     6061 tagtaatact caaaaaacag accgtaatca ccgttcatct tctctgcacc tccggaccgt
     6121 aatcctttgc aattctaagt ctattttacc atatcgctca caaaacgaag aaagaaagcc
     6181 taaccaattg ataaaagtga ctgccaaata ctgaaaattg aacaattacc tgaaagctga
     6241 aacaaacatc ctacatagaa tttattaaaa tttatacaaa ttccggtcag ttgatataaa
     6301 aatattcagt tatttaatga cgttttcgcg ctagggtgct atactataca tgaaatgacg
     6361 ttcgtcctat tatcctacaa acatggcgca gtatccgaag attccgacgc tacaaggatt
     6421 tttgcgaaaa gcacccatcc cttcacgtcc ccgtccatca cttattgatg aaaagaaatc
     6481 atgatttaaa aaaacaaata tctttacatc agttatattg ccaatttaat tttctnnnnn
     6541 nnnnncctat gaaacattgc taaataaaca ctcaattgca aagcgctata gttattttgt
     6601 ttttcatcta tttttaatga acaaaagcaa gcgttatgtc cattatgttc cggtacattt
     6661 tttaattgtc ccaagaccaa aaaaatgtgt tgtaccggcc attaaattgc atgccaaaaa
     6721 tagataatga ttactctagc ccggcacttt cctgtaatcg gactcgcttg ggatgatccg
     6781 atccgattcc ttaatccagg tgttttgctg gcgaaatatt tgtcccatct gccgcatcaa
     6841 taaattggtt ttgttaacga caacatacta ctcgcaataa gtttattttt cctagcctat
     6901 catttgtaca tgtcggatta aattacagac agattacaac ccaggctttt tgtgacaagg
     6961 attaaatttt attatttttt taaaatatac tagacaaaaa aaaaaaatag tatattatta
     7021 ttgttattgg ctcgctcaga tgatatggaa tttgagtcaa ttaattttca aattgtcgaa
     7081 ccgttgctat cagatagatt acgtcaggag gattactatg tacacaatgt ccaacataaa
     7141 aaacctctcc ttgaagattg ttcttgctga aaagaataca atcacatcag cccaacctac
     7201 aaactaccaa aaattccctt atgttaaact tttctatgtt acaacagggg atgtttcttt
     7261 agtcatgaaa gaaacaacag aaaagctttc aaaagatcaa ctgatcttat tgaacccaaa
     7321 tgtagagtat tattgggacc tgacaggaga taaagcagca gagattatcg aagtcggcat
     7381 ccacgggctt gaattctcca aaatcggtga tgcagaaagc gatttctctt actaccgcca
     7441 caacgacggt gaaaaagaaa ctgcgacttt cttgaattta ttgctggatg aaatgagcac
     7501 acgcagccag ggatacgaag aggtcagcaa acgtttcgtc gagatcatcc tgatccactt
     7561 gttgcgtctg gaagaatttt cgatccggaa ctcactgaac aaaaagaacc acaaagaaat
     7621 ccaaacggtc aagaattaca tgaaagtgaa ctaccacaag aacatcacgt tggatgactt
     7681 agtggatctg gtccacatca acaaattcta cttgatccgt atcttcaagc aagaagtcgg
     7741 catgtcgccg atcgactact tgatccatat ccgtatcgac gaagcgcaaa aaatgttacg
     7801 caacaccaat atcgcaatcg ccgatatcgc gcacttggtc ggcttccagt ctccgtcaca
     7861 cttctcgaaa acattccggg aattgtcgaa ctgcacgcct tcacagtatc gtcgtcaagg
     7921 aaaccaataa tacaacaaaa agaagaagaa gccgctgttt taagcgactt cttcttcttt
     7981 ttgttcctcc aatgcccgca aatagtctct tgcttcgcgc ggtttgcgga aatgttgaat
     8041 ttcgatgccg gccggcgcct gatccagtaa agcaatcaaa cccgggcggt tgtttttgtt
     8101 gaagttccag atgaatttca taaattccca atcgaatcgc tccgggcagc cttcccccat
     8161 atcaggacgg gtccttttgc ggttcaaaac aatccgcttc aacgcgcgat agagacacaa
     8221 actccttgga tagtcgaaaa agaaaactgt atccgcacgc tccaaccgaa tcggcaggct
     8281 ccgttggtaa ttgccgtcga ttatccattt ttcgtctttg atccgttctt tttggatccg
     8341 atcctgttct tcagcagcgc ggagattcca atcaggaagc caatacagtt tatcgaggtg
     8401 atgcaacgga ttgcccgtca cttgctttaa cttgcgcgaa aaagtagact ttcccgatcc
     8461 gctgcatccg atgacgacga tccgtttcat tgccttccca ctcccgctgt ccttcattat
     8521 tgccaaagcg attcctccta tcgaacgaga cattctcttc tgttgaacag tttaccacag
     8581 cgcaattacg gtggacagta cctgctatgt ccccgcaact aatggtatac ttggactaca
     8641 gaacttgaaa ggaaagataa ataatgatac gcaaagcaat aaaagaggac tgtccggaat
     8701 tgtatgatct gatccacgaa atcttagtgg acatggaatt gccactgctc accctgttgg
     8761 aaccggatgt cctaaaagcc gcagtggtcc gggctatgca ggaggacgtc taccggttca
     8821 gttaccggaa tggccttgtc tatgaagaaa atggcgaaat tctcggctgt tgtttcggct
     8881 atccgggagc gtccgaacct atcatcgatg ccgcactcca gaccgctttc gataagatgt
     8941 acatcacggc agccaggctt ttccctgata gcgaaacatt tgccggagaa tggtatctgg
     9001 attccatcgt gacgaagcag tcagcgcgcg gaaaaggcgt cgcttaccaa ttgctgcaag
     9061 cactgccgac actcgcctcg gccgcgggtg aaaccgtcat cggcctgaat tgcgaaaaag
     9121 acaatctgca ggcaaaaaaa ctctatgaga aagtcggctt caggacagtt tccgaacgga
     9181 ctttgagcgg gcatgtctat gaccatatgc agtgggactt cacatataac taacaaaaaa
     9241 atcagccgaa acatcactgc ggctgatttt tttgcgattt caggtcaaat agaaaggtgg
     9301 agccttttcc cggggtgctt tcgaccgatg ccgtggcgat cagctatctg cttggcgacg
     9361 gccaaaccca gaccggtgcc ttgcttgttg ctttcttcgt ttgatttgaa gaacttttcg
     9421 aagatgtacg gttgatcctt ttccgggatt cctggcccgt tgtcagtgat agccaatcgg
     9481 cagccctcac ctgcgccagt caacgtgatc cggatttcgc tcttttcggc tgaaaatttg
     9541 acggcatttt ccatcacggc aagcagcatc tgccgcagcc gggtataatc ccccatcagc
     9601 atgaacgcca acggctcttt cgagaaagtc agcgtaatcc ccttttccct ggcgatatag
     9661 cgcacggaac ggatggcatt ctccacgaca tccacaaaat tcaaggaact catctccatt
     9721 tgatagtccg ggttctgcag ccgcgacagc tccagcagat cgttgatcat ccgctccaga
     9781 tggatgcttt cggtcaacat ctgttggtgg tattcatcga ttttggcggg atcctcgacg
     9841 acatgatcgt tcaaggcctc cagcgagccg cggatgaccg tgaccggcgt gcgcaattca
     9901 tgggaaatgc tcgtgatgaa atccttgcgc atcttatcca acttggcgct ttcttgcgca
     9961 gcctcagcca acttgtccgc cagaatatcg atgttggcgg caagcgaacc catttcatca
    10021 ttttggatga tccccgtatg ggcttcgtaa tggttatccg ccaactgctg tgtcgtttct
    10081 tccatctttt tcaacggctg gatgaaccgt ttcgccaaca ggaccgctat cccgacggca
    10141 gcgatcaaag ccaaagccaa gctgattccc aaggtagaaa atcctgaccg tacggcagtc
    10201 tgcatgtttg cgatatagtc gtgcaaaagg accgcagcga tgacctcccc ttctgcggag
    10261 agcaccggcg ccccgaccgt gatggaggta tcctccaata acgggctgaa gccttcgctg
    10321 aaggcgacgt ccccttcgaa cacctcatcg acaagcgcca gtgcagcgcc tggtagttcc
    10381 ccataggtga tctctttttt gccggtcccg acactgatcg tctccgcgga ttgatcgaca
    10441 atccacacct cgcccatcgc gatatcctga atggccgtga tataggcgtt gtatccgttc
    10501 acattgttgc gcccggtcgt cccagtggca ccattttgtg cgag
//
LOCUS       DAOMUJ010000013        10542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1520_length_10542_cov_2.571660, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000013 DAOMUJ010000000
VERSION     DAOMUJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_10542_cov_2.571660"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLT73_00800"
     CDS             complement(<1..270)
                     /locus_tag="PLT73_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18950.1"
                     /translation="MKDVAKEKLAEFIDRYNEQKYLLQTASEQTMRSWIDNFLQIFDW
                     NCQDVNQVQQEIMVTREEKQKLKLIGSKHSKPDYTLKNGRVRVNFL"
     gene            complement(538..1017)
                     /gene="rlmH"
                     /locus_tag="PLT73_00805"
     CDS             complement(538..1017)
                     /gene="rlmH"
                     /locus_tag="PLT73_00805"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRM18951.1"
                     /translation="MNIKIITVGKLKEKYLKEGIAEYTKRLGSYCKLQIIEVGDEKAP
                     ENLSDKEMEMIKDKEGEKILAKIPEQSYVFAMAIQGKQYDSVEFANEIDKLGTSGKSD
                     IVFIIGGSLGLSQAVLSRANQHISFGKLTYPHQLMRLVLVEQIYRAFRIIHGHAYHK"
     gene            1342..2721
                     /locus_tag="PLT73_00810"
     CDS             1342..2721
                     /locus_tag="PLT73_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM18952.1"
                     /translation="MLKKFSSWSVSQKIATSFAFVIFTGSILLTLPISQLPTSNATYF
                     DNLFTAISMVCVTGLFTESVHDSYTIFGQVVGMALMQISGLGLMTLIASVITRLKGRI
                     GLRNRLAVQEGINRGDARGFREYLSDILKYTAVIELTGFVLLSIRFVPEMGWPLGLFT
                     SLFLAISAFCNAGFDPMGNVSLVDYVHDPLVNYVITSLIILGGIGFSVWFDVSKSVKC
                     YFAKKCRPSLKVFFRGLSLHSRLAVSVSLVLILVGLVFFVAVEYNNPNSIGTFTFPQK
                     LLAGYFQTVTMRTAGFATIDYTTVYPFTLFWFVLTMFVGGSPGGTAGGLKTTTFAIIF
                     LTLYNELRGQSNVNIANHTISRPQVRQAFVIFMALLGTLILGTSVLSILNPEVDFIYI
                     LFEAVSAIATVGVSANLTPTLGELSQAVIMVLMFAGRIGPITMLDSLVRRTQKVQDVE
                     FSKGTILIG"
     gene            2746..>3234
                     /locus_tag="PLT73_00815"
     CDS             2746..>3234
                     /locus_tag="PLT73_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM18953.1"
                     /translation="MSKLVGILGLGIFGSTIAKTLSEFDSDVIAVDKDPENVNRLEPF
                     ITKGIIGDVTDLALLESIGLSDCDVVVIATGTVLEASVLAVMNCKKLGIKHIIAKAKN
                     KNYREVLEAIGADTVILPEKETGMRVAKNILRTNIEDVVNLDEQTSIIEFYPPAKWVG
                     RTL"
     assembly_gap    3235..3253
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3254..3432
                     /locus_tag="PLT73_00820"
     CDS             <3254..3432
                     /locus_tag="PLT73_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM18954.1"
                     /translation="DLDLRNKYDINIIGLRKYRGEHLNVSFSGDYIVDKDIVLVGITE
                     SDVFERYDYLNQLT"
     gene            complement(4122..5618)
                     /locus_tag="PLT73_00825"
     CDS             complement(4122..5618)
                     /locus_tag="PLT73_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM18955.1"
                     /translation="MDRDEEKKNNPFEQDAHNETETEIESTAADAASADHIQEDLSEE
                     KEIEAEYTEIEETASEEEEPIVEPAARATMNKEERARVYNNVSETEESSRRPKDRKNS
                     PIKNGIVGGLIGGGMVALIGAGLLFGSGILGNGSGTDDAGNPAINETVKDIAVNVTTD
                     TTAAVEKVQDAVVSIINMQAGNTNMFGFDLPQSSSDTTNGTLETSSEGSGVIYKIEGD
                     LAYIVTNNHVIDGADELEILMKDGTKEVASVVGSDIWTDLAVLTIPAANVTAVASFGD
                     SDAVNVGEPAIAIGSPLGTEFATSVTQGIISAKNRSVSTDVDSDGIIDWDVTALQTDA
                     AINPGNSGGALINIAGQVIGINSMKISDSNVEGMGFAIPSNDVLTIINELERNGEIVR
                     PILGVSMLDLSQISASQQSSVLKLPEDVTEGVVIAEVQALSAAEMGGLKQYDVITEMN
                     GEAITSIVDLRKILYALEVGTDVEISYYREGTLQSTTVTLTEGQTSAE"
     gene            complement(5802..6590)
                     /locus_tag="PLT73_00830"
     CDS             complement(5802..6590)
                     /locus_tag="PLT73_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM18956.1"
                     /translation="MHVSYLASGSSGNCTYIETPKRKILVDCGLSGKKIAGLMEIIGR
                     NLADVDSILVTHEHRDHVHGLGVLARKYHMDLYANEATWKAMDHIIGNVKTEQKHIFE
                     MGQVLTLGDVDIQSYGVSHDAAEPQFYSFHHEGKQMVMLTDTGYVSDRLRGLLRDADA
                     YLIESNHDLELLRMGGYPWSLKQRILGDEGHLSNEDGAAAVAEMLGDRTKRIYLGHLS
                     RDNNIKTLAHHTAESILMQKGLGVNEDFFVYDTDPDEPTELFRV"
     gene            complement(6958..8478)
                     /locus_tag="PLT73_00835"
     CDS             complement(6958..8478)
                     /locus_tag="PLT73_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM18957.1"
                     /translation="MKEKERNELLSLYEEMKALRQAVYEEGNGLYAEWKSGISRTAFE
                     LSAKNLAYYLALRRRDIRPLQNRLSKWGLSSLGRTESKVLQQLDAIVRNLALMSGAIK
                     QLSDYPKTPEKFPGRKKLATEATALLGDAPKHRTTRIMVTLPEQAATDKDFLVRLLER
                     GMDIARINCAHDDSAVWLKMIENLKEAQKTAGKSCRIYFDIAGPKIRVDAVSSEKEKP
                     RLKVGDLFFLTADASFKDLKHYPAIVFSESLNLFDELKVDSPVILDDGVLEGRVVEQK
                     SQGVVVQVGKTAKAKGIRIKPQKGLNFPESTFRMPILTDKDREDLLVAEIEADILGFS
                     FVRGSKDIVAIQNTLAEQVGSEAALAIPLVIKIETVEAVNHLPELVVAAASKNPLAIM
                     IARGDLAAEAGFLRLSELQEEILWICEAAHIPVIWSTQVLENMVKNGVPTRAEMSDAT
                     MAGQAECVMLNKGDFVEEGIALLDAILVQSQQNRSKKTAKLRALKIAKKAWRKTKK"
     gene            complement(8611..10476)
                     /locus_tag="PLT73_00840"
     CDS             complement(8611..10476)
                     /locus_tag="PLT73_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRM18958.1"
                     /translation="MKRIGSIVLVMFFFLIGFGQKEVQARSYEITNYDVLVEIQRDGS
                     AFFTESITYDFEGEFNGVLYELDISEVADPTDVKVSMQGYLSETPFPFALSDTEESGT
                     FELDNTGDYLNFTVYNKMTDEIQTVIYQYRIPEIITNYNDIAEFNRKVIGSAWEDPLN
                     DVDVTILLPEATAEEELRAWGHGGGENSTVTLEDNQKALLYVPQNPANQFVEAHVIFP
                     TRITADNPNVVNEDKFDEILAQEAALAEEEERNALLFGVLSAVFAAFAPILPILVFLW
                     VRRARKREIPQEIHLPDHIYELPEDMTPAVMNGAVFSGNVQAADISATILDLVRKGYL
                     TISPVQIPQKGIFGREKAPEDSFLLTQIRDTKNAPLLSHEKQLLEWFISDVGNGESVT
                     LDDIENIADNSAEAKRFNENREKWQERVMDVATPKRKTYRSKDNKKAVAFAALALVAN
                     IFLVIAVVFFGAMSGNFTAWAIVLAVLGAAFSFIQLMAVAVKPIMTAEGERTKQEWNG
                     FRNMLKDVGDFPMREVGSIALWDHFLVYAVSLGVADKVMKQMQVEFPVNEIQASAFGS
                     YYYMNNAIFLSSLNNSINTGVTKSMPTSSNSSGFGGGFSGGSSGGSGGGSGGGAF"
BASE COUNT         2440 a         2690 c         2374 g         3019 t
ORIGIN      
        1 caaaaaattt actcttaccc taccattctt caaagtatag tccggcttag aatgtttaga
       61 gccaattaat ttcaattttt gcttttcttc ccttgtaacc attatttctt gctgaacctg
      121 gtttacatct tggcaattcc aatcaaagat ctgtaaaaaa ttatctatcc atgatctcat
      181 agtctgttct gaagctgttt gtaataaata tttctgctca ttatacctat cgataaattc
      241 agctaatttc tcttttgcca catctttcat aatagcctcc tatgttataa taatggtaaa
      301 ttcattgtaa cataggatat cactggaaaa tttctgtgta ttaagtatta gctatatata
      361 ttttagataa ataacttata gcaaagaatc aattgattct ttcatagaac agaggatttg
      421 tccccgtaat atatgataaa caagttcact atgggtaata acaatagagc catcatcgta
      481 gtgaggcgat gatggctcta actatcgtca ataattatta atgcgaaaaa acctcattta
      541 tttatgataa gcatgcccat ggataatccg gaacgctctg tagatctgtt cgacgagcac
      601 gaggcgcatg agttggtgcg ggtaggtcag tttgccgaag gagatgtgct ggttggcgcg
      661 ggacaggacg gcttggctga ggccgagcga gccgccgatg atgaagacga tgtcgctttt
      721 gccggaggtg ccgagtttgt cgatttcgtt ggcgaattcc accgaatcgt actgtttgcc
      781 ctggatcgcc atcgcgaaga cgtagctctg ttccgggatt ttggcgagga ttttttcgcc
      841 ctctttgtcc ttgatcattt ccatctcttt atcgctcagg ttttcgggcg ctttttcgtc
      901 cccgacttcg atgatctgca gcttgcagta gctgccgagc cgctttgtgt attcggcgat
      961 gccctccttc agatatttct ccttcagttt tcccaccgta atgattttta tgttcattgc
     1021 ttttcgctcc tttttcttct caactactat cctatgggaa caagtgcgat ttcgcaaccg
     1081 tctttttttt aacggctttt tgacagaaat atatctatga ttttctgttt atcttattat
     1141 taaccaaact ggttgtacag tcttattata atatgaagga aagtgcaatt tttgtctgaa
     1201 aaaggaatga aaaccagttg cttttttccg taaatgattt aagatacatt tatgcttttg
     1261 agttgcttaa tcatgcgata gttttataat gaagtaacaa atcctatccg catgctattt
     1321 gagaggagaa ttggaaccat tgtgttaaag aaattttcga gttggtctgt ttcgcaaaag
     1381 atcgcaacga gctttgcctt tgtcatcttt accggatcga ttttacttac gctgccgatc
     1441 agtcagctac ccacttcgaa tgcaacctat ttcgacaacc tattcactgc tatatcgatg
     1501 gtttgtgtga ccggcctgtt caccgaatct gtacacgact cgtacactat cttcggacag
     1561 gtcgtcggga tggccttaat gcaaatcagt ggattgggat tgatgaccct gatcgcttcc
     1621 gtcattacgc gcctgaaagg caggattggt cttcggaacc gtttggccgt acaggaaggg
     1681 atcaaccgtg gtgatgcgcg tggattcagg gaataccttt cggatatcct gaaatatacc
     1741 gctgtcatcg aattgaccgg tttcgttttg ctttccatcc gctttgtgcc ggaaatgggt
     1801 tggccgctcg gtctttttac ttccctgttc ttggcgattt cagcattttg taatgccgga
     1861 ttcgatccga tgggaaatgt cagcctcgtg gattatgtcc atgacccatt ggtgaactat
     1921 gtcatcactt cgcttatcat tttgggcggt atcggctttt cggtctggtt cgacgtatcg
     1981 aaaagcgtaa aatgctattt tgccaaaaaa tgcagaccaa gcttgaaagt ctttttccgt
     2041 ggcctgtcgt tgcatagccg ccttgctgtc agcgtatcgc ttgtgctcat ccttgttggt
     2101 cttgtcttct ttgttgcggt tgaatacaac aatccgaatt cgatcggaac attcaccttc
     2161 ccgcagaagc tcttagccgg ttattttcag accgtcacca tgcgtactgc aggattcgcg
     2221 acgatcgact atacaaccgt ttaccccttc acacttttct ggtttgtgtt gaccatgttt
     2281 gtcggtggtt caccaggcgg gactgctggc ggtctgaaaa ctacgacatt cgcaatcatc
     2341 ttcctcacgc tctacaacga gttgcgcgga caaagcaatg tgaatatcgc aaaccataca
     2401 atcagtcgcc cacaagtccg tcaggcgttc gtcatcttta tggcactact gggaaccttg
     2461 attctcggaa caagcgtatt gagcatcttg aaccctgaag tggattttat ttacattctg
     2521 tttgaagcgg tgtctgcgat tgcgacggtg ggcgtcagcg ccaaccttac tccaactctg
     2581 ggagagttga gccaagccgt tattatggtg ctgatgtttg cagggcgtat cggtccgata
     2641 accatgttgg acagtctcgt cagacgcacg caaaaagtac aagatgtaga gttttctaaa
     2701 ggaactatcc tgatcggata atagcaaaga ggagcgtatc taaatatgag taaattggtt
     2761 ggaattttgg ggcttggtat tttcggatcg acgattgcaa aaacattgag cgaattcgat
     2821 tcggatgtta tcgctgtcga caaggatcct gagaatgtta atcgtctgga accattcatc
     2881 acaaagggaa tcataggcga cgtaaccgac ctggctttgc ttgaatcgat cggtctttca
     2941 gactgtgatg tggtcgtgat cgctaccggt acggtgttgg aagccagcgt gttggctgtc
     3001 atgaactgca agaaactcgg catcaagcat atcatcgcca aagcaaaaaa caagaattac
     3061 cgtgaagttc tggaagcgat cggcgcagac actgttatcc tgcctgagaa agaaaccggg
     3121 atgcgcgtcg ccaagaacat tctgcgcaca aatattgaag atgtagtgaa cctggatgaa
     3181 cagacttcca tcatcgagtt ctaccctcct gctaagtggg taggccgaac actcnnnnnn
     3241 nnnnnnnnnn nnngagattt ggacctgcgt aataagtacg acatcaacat tatcggtctc
     3301 cgcaaatacc gcggtgagca cctgaacgta tcgttctcgg gcgattatat tgtggataaa
     3361 gacatcgttc ttgtgggtat cactgaatcg gatgtttttg aacgttacga ctatttgaat
     3421 caactgactt aacttactgc agcatcccat tattggggtg ctgctttttg tttgataatc
     3481 agaatcggat tcagattccc attttttagt ttccagctgc tgttgtaccc acttcacaca
     3541 ctcaaatcac accttatcca cattagttat ccacatgtac acacgttccc ccgttattta
     3601 gtggtagcat tattttatgg cacatcatct tgattatctt taaaacgaat tatttcccta
     3661 tacacgtagt tatgcacacc gcctattgag caatcttata gatctgaact ggccatttaa
     3721 caacgtttaa aaacataatt tcacatgcag acataatttt atgcacaggt ttatccattt
     3781 tctgtggata acttccgttt tcagaaagca ctgttcccat aagcatttac gcgttttgca
     3841 cacaaaaact ttttttccac agaatttatc cacatccatg tggatttctg cgcttttatg
     3901 ctttattttc tggttggaag atggataact tggatttatt catcttttcg accgaatttc
     3961 agcccaagag catggattcc tccgacttat tcatctttcc ggatccaatc cccttcccag
     4021 ccccctgtca ccaacaacta cagaccacaa atccaaaaca ccccgcacga ctgctctcaa
     4081 gagccgccat acggggtgtt cgatattgct gtttattcag tttattcggc tgaagtctgt
     4141 ccttccgtca gagtgacagt cgttgactgc agcgtgcctt cacggtaata ggagatttcc
     4201 acatcggttc ccacttccag tgcatacagg atcttgcgca ggtcgacgat gctcgttatg
     4261 gcttcgccgt tcatttccgt gatgacgtcg tattgtttta agcctcccat ttcggctgcg
     4321 gataaggctt ggacttctgc aatgacgaca ccttccgtaa catcttcagg caatttgagc
     4381 acggaacttt gttgggacgc tgaaatctgg gaaagatcca gcatggaaac tcccaggatc
     4441 ggacggacga tttcaccgtt ccgttccagt tcattgatga tggtcaagac atcgttgctt
     4501 gggattgcaa agcccattcc ctcaacattc gaatcggata ttttcattga attgatgccg
     4561 atgacctggc cggcgatgtt gatcaaggca ccaccggaat tgcccgggtt gatcgccgca
     4621 tccgtctgca gtgccgtcac atcccagtca atgatgccgt cgctgtccac atccgtcgag
     4681 acggaacggt ttttggctga aatgatgcct tgcgtgaccg aggttgcgaa ctcggttccg
     4741 agtggagagc cgattgcgat ggccggttca cccacattga cagcgtccga atccccaaag
     4801 gaagccactg ccgtgacatt cgccgccgga atcgtcaaga cagccaagtc ggtccagata
     4861 tcgctgccga cgacactcgc tacttctttc gtgccgtcct tcatcaggat ttccagttcg
     4921 tcggctccgt cgatgacgtg attgttggta acgatatagg ctaggtcacc ctcgatctta
     4981 tagatgacgc cgctgccctc actggatgtt tccagagtac cgtttgtggt gtctgaggag
     5041 ctctgcggca aatcaaaacc gaacatattc gtgttgcccg cctgcatatt gatgatggag
     5101 acgaccgcat cctgcacctt ttccacagcg gcagtcgtgt cggtcgttac attgacagcg
     5161 atgtccttca cggtttcgtt aatggctgga tttccggcat cgtccgttcc ggatccgttg
     5221 ccaaggatac ccgagccaaa aagcaagccc gcaccgatca gggcgaccat cccaccaccg
     5281 atcaacccgc cgacaatgcc attcttgatc ggtgaatttt tacggtcttt cggacgtctg
     5341 gagctttctt cggtttcgct gacgttgttg tatacgcgcg cccgttcctc tttgttcatc
     5401 gtcgctcttg cggctggttc cacaattggt tcttcttctt cgctggccgt ttcttcgatt
     5461 tcagtgtatt ctgcctctat ttccttttct tcggataagt cttcttgaat gtgatcagca
     5521 gatgcagcgt cggcggcagt cgattcaatt tcagtttccg tttcattatg cgcatcttgt
     5581 tcgaacggat tgtttttctt ttcttcgtcc ctgtccatat tgattcctcc ttatcgttct
     5641 acaggctcag tatagttcct tattttgaat aaattatgaa gatagtgaga gatatttcag
     5701 aaaatcgctc ttcagtaagc ttattgtatc atatccgcgt acgcaaaagg gagcgaaatc
     5761 actcgatccg cccctcctgc acttgccatc ccgcctgtcg cctacactct gaaaagttca
     5821 gtcggctcgt ccggatcggt atcatataca aaaaaatctt cattgacacc caggcctttt
     5881 tgcatcaaaa tgctttcggc agtatggtgt gccagggtct tgatgttgtt gtcccggctc
     5941 aaatgcccaa ggtagatgcg tttcgtccgg tcgcccaaca tttcggcgac agcagcagct
     6001 ccgtcctcat tggaaagatg gccttcatcc cccaaaatac gctgtttcag tgaccatgga
     6061 tatccgccca tgcgcagtaa ttcaagatca tggttgcttt caatcaggta tgcatccgca
     6121 tcccgcaaaa ggccgcgcaa acgatcactg acatagccgg tatcggtcaa catgaccatc
     6181 tgtttgcctt catgatggaa ggagtagaac tgcggctcgg ccgcatcatg cgaaacgccg
     6241 tagctttgga tatcgacatc gcccaaagtc agcacctggc ccatctcgaa aatgtgcttc
     6301 tgctcggtct tgacgttgcc gatgatgtgg tccatcgctt tccaggtcgc ttcgttggca
     6361 taaagatcca tgtggtactt gcgcgccagg acgcccaggc cgtgcacgtg atcgcggtgt
     6421 tcatgcgtga cgaggatcga atccacatcc gcaaggttcc ggccaattat ttccatcaga
     6481 ccagctatct tcttgccgct caacccgcag tcgaccaata ttttccgttt cggcgtctcg
     6541 atgtaggtgc agttgccgga actgccgctc gccagatagc tgacgtgcat gccctctttt
     6601 tctattgttt caatcacact tagcgcctcc aatacatcca taagtttaac ttattttttg
     6661 gaaaatgcca gtaatttttc tcagtgttaa gaaaagttaa tcttcaggcg ctgcatgggg
     6721 aacattctta atttaaggac attgacaaac aggagtcgag gggaagcggt gctaaatttg
     6781 tcggataggt gcggtagatt cgacaatttg gtaccagttt gagccgagat agtcgaatgt
     6841 cgagaaactt tcgacaattt gacgtatttt cggattgaaa ttgtcgaata gctgaggaac
     6901 attcaacaat ttggcccctg tagtcaaaaa aagcgagaat gcatgatgca tcctcactta
     6961 tttcttggtt ttgcgccagg ctttcttggc gattttgagc gcgcgcagtt tggcggtctt
     7021 cttcgatcgg ttctgctggc tctgtacgag gatcgcatcc aacaaggcga tcccttcttc
     7081 cacgaaatcg cccttgttca gcatgacgca ctccgcctgc ccggccatcg tcgcatccga
     7141 catctccgcc cgcgtcggca cgccattctt caccatgttc tcgagtactt gtgtgctcca
     7201 gatgacggga atgtgggcag cttcacagat ccagagtatt tcctcctgca gttccgacag
     7261 cctcaagaag ccggcctccg cagccaggtc tccacgtgcg atcatgatcg ccaacggatt
     7321 tttgctggct gcagccacga ccaactccgg cagatggttc acggcttcta ccgtttcgat
     7381 cttgattaca agtgggatgg ccagcgctgc ttcgcttcct acttgttctg ccaatgtgtt
     7441 ttggatggcc acgatgtcct tgcttccgcg gacaaaggag aagcccagga tatcggcctc
     7501 gatttcggca accaataagt cttcgcggtc cttatccgtc aatatcggca tcctaaaggt
     7561 cgactccggg aaattgagcc ctttctgcgg ctttattctg atccctttag cttttgcagt
     7621 cttccctacc tgcacgacga cgccttgcga tttttgctcc acaacacggc cttccaggac
     7681 gccatcatcc aaaatgaccg gactgtcgac cttcagctca tcaaagaggt tcaggctttc
     7741 gctgaacaca atggccggat agtgcttcag atctttgaag gaagcatccg ccgtcaggaa
     7801 gaacaagtcc ccaactttga ggcgtggctt ctccttttca ctgctgacgg catccacccg
     7861 gattttcgga cctgcgatat caaaatagat gcggcaactc ttgccggcag tcttttgggc
     7921 ttccttcaga ttttcgatca tcttcagcca gacggcactg tcgtcatggg cgcagttgat
     7981 acgggcgatg tccatgccgc gctccagcag gcggacgagg aagtctttgt cggttgcggc
     8041 ctgctccgga agggtgacca tgatccgggt cgtccggtgc ttcggcgcat cgcccaataa
     8101 ggcggtcgcc tcggtcgcca gttttttccg gcccgggaat ttctcgggcg tcttcggata
     8161 gtcggacagc tgcttgatcg cccctgacat cagcgccaag ttgcgcacaa tcgcatccag
     8221 ttgttggagt actttcgatt ccgtccttcc caatgaggac aagccccatt tcgacagtcg
     8281 gttctgcaac gggcggatat cccttctgcg cagcgccaga tagtaggcca agtttttggc
     8341 gctcaactca aacgctgtcc gggaaatacc cgacttccat tccgcataaa ggccattgcc
     8401 ttcctcatag accgcctgac ggagcgcctt catctcctcg tataatgaca gcaattcatt
     8461 ccgttctttt tctttcattc cgcacccacc tcggcctttc atcatcttta aaacaaagga
     8521 ttgcctgctg aaatataact cggtatctga aacaaaaaca ccctgctctg cttcagaata
     8581 ggatgttttt gaaagtatgg atgacgctcg tcagaacgcc cctccgcctg aaccgccacc
     8641 ggaaccgccg gatgaaccac cgctgaaacc gccgccgaag ccgctgctgt tcgaggaagt
     8701 cggcatcgat ttggttacac ctgtgttgat gctgttgttc agcgaagaca ggaagatggc
     8761 gttgttcatg taataatagc tgccgaatgc ggaagcctgg atttcattca ccgggaattc
     8821 gacctgcatc tgtttcatga ctttgtcggc cacgcccaga gaaacagcat aaaccaagaa
     8881 atgatcccat aaagcgatgg atccgacttc acgcatcggg aaatcaccca cgtccttcag
     8941 catattgcgg aagccgttcc attcctgctt cgtccgttca ccttccgcag tcatgatcgg
     9001 cttgaccgcc actgccataa gctggatgaa gctgaaagcc gcacccaaaa cggcgagaac
     9061 gattgcccaa gcggtaaaat tgccgctcat ggcgccgaag aacacaacag cgatcacaag
     9121 gaagatattt gccaccaaag ccagtgctgc aaaggcaacg gctttcttat tatccttcga
     9181 acgataggtt ttacgttttg gcgtggcaac gtccattacg cgttcttgcc atttttcccg
     9241 attttcattg aatcgttttg cttcagccga gttatcggcg atattctcga tatcatcgag
     9301 ggtgacgctt tccccattgc cgacatcgct gatgaaccac tccaaaagtt gtttttcgtg
     9361 gctcaggagc ggagcatttt ttgtgtccct gatctgcgtc agcaggaagg agtcttccgg
     9421 cgctttttcc cgcccaaaga tgcctttttg gggaatctgc accggtgaaa tcgtcagata
     9481 gcctttgcgc acaagatcca atatagtcgc cgaaatatcg gcagcctgca cattaccgct
     9541 gaagacggca ccattcatga cggccggtgt catgtcttct ggcaattcat agatatggtc
     9601 cggcagatgg atttcctgcg gtatctctct ttttcttgct ctccttaccc agaggaacac
     9661 cagaatcggc aaaatcggcg cgaacgcggc aaaaacagca ctgaggacgc cgaaaagtag
     9721 cgcattgcgt tcttcttctt cggccaaggc ggcttcctgc gccaagattt catcgaattt
     9781 atcctcgttc actacattcg gattgtctgc agtaatgcgt gtcgggaaga tcacatgcgc
     9841 ctcaacgaac tgattggccg gattctgtgg aacgtaaagc aaggcttttt ggttgtcttc
     9901 cagcgtcacg gtactgtttt caccgccacc atggccccaa gcgcgtaatt cctcttcagc
     9961 tgtcgcttcc ggcaacagaa tggtgacatc cacatcgttc aatggatcct cccaagcgga
    10021 accgatgact ttgcggttga attcggcgat atcgttgtaa ttcgtgatta tttcgggaat
    10081 gcggtattgg tagatgactg tctggatctc atccgtcatt ttgttgtata cggtaaaatt
    10141 caggtaatct cccgtattat ccagttcgaa cgtccctgat tcctccgtgt cgctcaaagc
    10201 gaacgggaaa ggggtttccg acagataacc ttgcatcgaa accttcacgt ctgtcgggtc
    10261 cgccacctcc gaaatatcca attcatacag taccccattg aattcccctt caaaatcgta
    10321 cgtgatgctc tctgtaaaaa aagcgctgcc atcccgctgt atttcgacca acacatcata
    10381 atttgtaatc tcataggaac gggcctgtac ttccttctgg ccaaatccga tcaaaaaaaa
    10441 gaacataacc agcacaatcg agcctatccg tttcatcgtt accttcccct tccgataccc
    10501 tccatcaagc ttgtcgcttc tgataccccc attttaacaa ac
//
LOCUS       DAOMUJ010000014        10486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1539_length_10486_cov_2.883712, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000014 DAOMUJ010000000
VERSION     DAOMUJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1539_length_10486_cov_2.883712"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLT73_00845"
     CDS             <1..496
                     /locus_tag="PLT73_00845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRM18959.1"
                     /translation="TGVLAHEFAHIRNYDIRLSTVALAIAGLIAFLAQFSTRMMFWGG
                     GGRRRKSDNSGGAGIILLILSLLILVLSPFVATIIRLALSRNREYLADASAVEFTRNP
                     EGLKSALIKIATSEPMEAANAASASLYIVNPFKRMREGEAESDGLFSTHPSTANRIKR
                     LEAM"
     gene            724..1473
                     /locus_tag="PLT73_00850"
     CDS             724..1473
                     /locus_tag="PLT73_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18960.1"
                     /translation="MSEDMLYVHYETVSNYVLSKGIAVKDYTKLARKLPENLLLVDGR
                     KSIGTYDNHTGFQVIKGQENVKAFFQKGTDNQAAMGKWIDFVNVDMLHLLTPIEISEI
                     LYIAHAHTHLHSPFYYKLQNNYIYLTLPNGFIKMYYRYLEQFHELFAEAITVKLKEKV
                     NEKKRFYQKSKEIAPFPIERVKEFIPIFKEGAVISFRQMSIEGSTYCIPLFIAEDRYA
                     SIGGRFNEKDSVGTICYDMERSEWKIDIDFE"
     gene            1648..2733
                     /locus_tag="PLT73_00855"
     CDS             1648..2733
                     /locus_tag="PLT73_00855"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRM18961.1"
                     /translation="MKIYLIYGGKSAEHDISILTAHSIIKAIYFNYYEVVPVYITKAG
                     AWIQGQSLSEPVAFSENLYLEPGSEKRFADSQEGQSYGEVISPADIQSEDAVIFPVLH
                     GPNGEDGTVQGLFEVLNMPYVGCGVLASASGMDKIVSKHLFQQVGLPQVPYVAVTRND
                     WKNDREKIFIQCEGSLIYPMYVKPANMGSSVGISKATDLEELTAAIETAFRYDRRVVV
                     EQGIEAREIEIAVLGNDDVHTSVPGEIVKTKDFYDYEEKYGNEEVLLQIPAELPEEIT
                     AKLQNYATIAFRALDGSGLSRCDFFVTKNYEIYINEVNTLPGFTSRSMYPLLWEETGL
                     KYSDLVEELIQLALKRHEEKLSYQFVE"
     gene            2871..4247
                     /gene="murF"
                     /locus_tag="PLT73_00860"
     CDS             2871..4247
                     /gene="murF"
                     /locus_tag="PLT73_00860"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRM18962.1"
                     /translation="MKPLRIIDVVKAVGAIDYTSANRFAEINSVVFDSRKATSDSLFV
                     PLQGETDGHDYVQSAIKNGATATLWSRPAGEAPEEIAVILVDDTLEALQKLAEAYLNM
                     IQPKVVAITGSNGKTTTKDMTAAILSARFRVHKTEGNYNNEIGMPMTILEMPEDTQVL
                     VLEMGMSNFGEISLLSRLAKPDIAIITLIGDSHLEFLGSRLGIAKAKMEILEGLKPEG
                     TFIYPGDEPLIADELAEESHFRQLTFGTDETAAVYAYDIVPGKTRTTFHVNLDPSVDL
                     EIPVLGVYNVRNALAALAAAQVLDMPLAEVKEALATFQLTANRTQWLDGVNGSQLLND
                     AYNASPTSMTAVLRSFAKLPREGKKIAVLGDIRELGERSAELHAGLAAEISPEEFDQV
                     YLYGPEMGALYENLRTVFPEGTLYHYVGEKTPLVEKLKSELQKADQVLIKSSFGTDLL
                     AVVAALRV"
     gene            4653..6221
                     /locus_tag="PLT73_00865"
     CDS             4653..6221
                     /locus_tag="PLT73_00865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM18963.1"
                     /translation="MKFSELGLNAELLKSIEQMGFEEATPIQEQTIPLALQGLDVIGQ
                     AQTGTGKTAAFGLPMLQKIDNNNKVIQGIVIAPTRELAIQTQEELYRLSRDKKVSVQV
                     VYGGADINRQIRALKNKPQIIVGTPGRLLDHIKRKTIRLDHIETLVLDEADEMLNMGF
                     LEDIEAILSETPAERQTLLFSATMPDSIKKIGVKFMHAPEHVKIKASHTAANLIDQYF
                     VKCKDFEKFDTMTRLFDVQNPELAIVFGRTKRRVDELSKGLELRGYRAEGIHGDLTQQ
                     KRMSVLNAFKTGDLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KEGMSVTFVTPNEMDYLRTIENLTKKPITPLQPPSDEEAFRGQVKTAMDEVETLVKET
                     ETDKYKRAAVRLLEEHTAEDLVAAFLKSLSKDATDVPVKITPERPLPSRKGKGGGGYK
                     GKGGGGYKGNRSSSDRRGGGERGGERSFNKNRSSDSKPRSGGKRYDDKRGGGSSASAP
                     KKSSHSFTIRNNND"
     gene            6343..6693
                     /gene="acpS"
                     /locus_tag="PLT73_00870"
     CDS             6343..6693
                     /gene="acpS"
                     /locus_tag="PLT73_00870"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRM18964.1"
                     /translation="MIYGIGLDVTELDRITSAYERRSDFAERVLTEAELKLFLVLSGS
                     RQMEFLAGRYAAKEAFSKAYGTGIGKLSFQDIEILPGEYRKPEITKSPFEGKGFVSIT
                     HSGNIIAAQVVLEK"
     gene            6708..7826
                     /gene="alr"
                     /locus_tag="PLT73_00875"
     CDS             6708..7826
                     /gene="alr"
                     /locus_tag="PLT73_00875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM18965.1"
                     /translation="MVVGKHRPTVETIDLEAISWNIQQIRKSLSPEKKFFAVVKADAY
                     GLGAVPVAKKAKEAGVDGFCVALLDEAMELRENGLSDDFIMVVGPTESEDAALIADNG
                     ISVAVTAKEWLDDALPYLNRRKTELPIRVHLAIDTGMGRIGLRTVEEVKAFEARIAEL
                     DDFVFEGIFTHFATADGEDPTLVDRQLALFEDILNALAQRPIVHLANSAISLWHEAFQ
                     TDAVRVGIAMYGYNPSDTVLELPYELKPSVQLDTHISYVKQMHEGDTIAYGARYRAFE
                     GEWIATLPIGYADGWRRDLRHQTVIVDGQRCPIRGVICMDQCMISLPKEYPIGTKVTL
                     LGENGGLVNNPSEMAVTIDTIGYEILTGINARVPRIYK"
     regulatory      8217..8262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8269..8757
                     /locus_tag="PLT73_00880"
     CDS             8269..8757
                     /locus_tag="PLT73_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRM18966.1"
                     /translation="MNTRKMVINTIIAALYVAMTGIFAFMSFGAVQFRVSEMLNHLVG
                     YHKNYKYGVLAGVFIANLIFSTLGAWDLVFGFGQTLISFLILEKLFKPNDSEMKRMMM
                     TAFVFTMTMVLVAIELYIVLDLPFWFSFATAAFGEAVVLVVSAPLMKYLNKIIGFAEK
                     MA"
     gene            8902..9549
                     /locus_tag="PLT73_00885"
     CDS             8902..9549
                     /locus_tag="PLT73_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM18967.1"
                     /translation="MEMYAITVGPIQENCYFLIGDHENDTIIFDPGEQAEDIIAAIEE
                     NALHPIAIVLTHAHYDHIGALETIREKYDVPVYQNPIEREWLLDPQLNGSARNPNVPD
                     VRLAKEADVFFEEMGPYEIGEFRFEMQHIPGHSPGSTVFIFRENGFAIVGDVIFKGSV
                     GRSDLPGGSHETLIEGIQKYIVTLPGETVLFPGHGDPTTVREEIYSNPYLNGATR"
     gene            complement(9721..10362)
                     /gene="cbpA"
                     /locus_tag="PLT73_00890"
     CDS             complement(9721..10362)
                     /gene="cbpA"
                     /locus_tag="PLT73_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HRM18968.1"
                     /translation="MLIRSLAIPKRELTIVKESATLQDALDILEESGFRCVPILDETE
                     TIFRGNIYKMHIYRHKANGGEMNLPVTHLLKNATKFISIDSSFFKVFFTIKELPYISV
                     LNKDMTFYGILTHNALLNMLAQSWSIDVGSYVLTVIAPGQKGDLYHITKIINRYSSIA
                     SCISLDINREDALRRVLVTLPAGTPKQVLDSIVKALERKDFRVTEIENLQVDF"
BASE COUNT         2958 a         2220 c         2663 g         2645 t
ORIGIN      
        1 gacaggtgtg ttggcgcatg agtttgcgca tatccgcaac tacgacatcc gcctatccac
       61 tgtcgctttg gcgattgcgg ggttgatcgc atttttggcc caattcagca cccggatgat
      121 gttctggggt ggcggcggcc gacgacgcaa aagcgataac agcggaggag cgggcatcat
      181 attgctgatc ctttccttgc tgattcttgt cttgtcgcct tttgtagcca cgatcatccg
      241 tctggcgctg tcccgcaacc gcgaatattt ggcggatgcg agtgctgtcg agttcacgcg
      301 caatccggaa gggctgaaat ccgctttaat caaaatagcg acaagcgaac cgatggaggc
      361 ggcgaatgct gccagcgcct cgctctacat tgtgaatccc ttcaaacgga tgagggaagg
      421 tgaagcagaa agtgacggtc tgttttcgac acacccctca acggcaaacc gaatcaaacg
      481 attagaagct atgtaaatag cttctttttt tcggacgttt caatcaaatt gaaccaatat
      541 agttcgggtt ttgatttgaa aaccctttca atttgttgct gtgaaaaaaa ataggagaaa
      601 aaggcaaatt atctttgtaa aagatacgga tttgtaataa tattaacatg gtactgatac
      661 cttaatcggt tcggcaagta gtagaatgat aataagggat attttgggaa ggggggatcg
      721 ttcatgagcg aagatatgtt atatgttcat tatgaaacag tcagcaatta tgttttgagc
      781 aaagggatag cggtgaaaga ttacacaaag ttagcccgga agcttccaga aaacttgttg
      841 ttggtggacg gaagaaaaag tattggaaca tatgataatc ataccggttt tcaggtaatc
      901 aaaggccaag aaaacgtaaa agctttcttc caaaaaggga cggacaatca agccgctatg
      961 gggaagtgga ttgattttgt gaatgtagac atgttgcatc tcctcacacc gatagaaata
     1021 tccgagatcc tttatatcgc ccatgcgcac acacacttgc actcaccttt ctactataaa
     1081 ttacaaaata actatatcta tctcacgttg ccgaatggct tcattaagat gtattaccgc
     1141 tatctggagc aattccatga gttgttcgcc gaagcaatca ccgtcaagtt aaaagagaaa
     1201 gtgaatgaaa agaagcgttt ctatcagaaa agcaaagaaa ttgcgccatt cccgattgaa
     1261 cgcgtgaagg aattcattcc gatcttcaaa gaaggtgccg tgatctcttt cagacagatg
     1321 agcatcgaag ggagcacata ctgcatccca ttgttcatcg cggaagaccg ttacgccagc
     1381 atcggcggcc gtttcaacga aaaggattcg gtaggcacca tctgttatga tatggaacgc
     1441 agtgaatgga aaatagacat agacttcgaa tgagcctaag ccgaaactta ggctttttct
     1501 gttttcattg aaccgaaatc agttggttca ttctaggtaa tagcaagcct actccatttt
     1561 tcacaaaaat cgttttagct aaaaaataaa tgtggtacaa tgttccttga gttaaagcag
     1621 ttatcaaaaa attcggagga tttctcgatg aaaatttatt tgatttatgg cggaaagagc
     1681 gctgaacacg atatttcgat cctgacagca cattcgatca taaaagcgat ctatttcaac
     1741 tattatgaag ttgtgcccgt ttatatcacc aaggcaggcg catggattca ggggcaatcc
     1801 ctttctgaac cagtagcatt ctctgagaat ctttatttgg agccgggttc cgagaagcgc
     1861 tttgccgatt cgcaggaggg gcaatcatac ggcgaagtga tttcacctgc agatatccag
     1921 tctgaagatg ctgtcatttt cccggtattg catggaccga acggggaaga tggtacagtg
     1981 caagggctat ttgaagtgtt gaatatgcct tatgttggtt gtggtgttct tgccagcgcc
     2041 agcgggatgg ataaaatcgt cagcaaacat cttttccaac aggtagggtt gccgcaagta
     2101 ccatatgttg ccgttacccg taatgactgg aaaaatgacc gcgagaagat tttcattcaa
     2161 tgtgaaggta gcctgattta cccgatgtat gtaaagccag ccaatatggg ctcaagtgtc
     2221 ggcatttcca aagctacgga tcttgaagag ttgaccgctg caatcgagac ggctttccgc
     2281 tatgaccgtc gcgtggtcgt ggaacaaggc atcgaagccc gtgaaatcga aatcgctgta
     2341 ttgggaaatg acgatgtcca tacatccgta cctggtgaaa tcgttaaaac caaagatttc
     2401 tacgactacg aagagaaata cggcaatgaa gaagttttgc tgcagatccc tgccgaatta
     2461 ccggaagaaa tcaccgccaa gttgcagaat tatgcaacaa tcgctttccg agcgcttgac
     2521 ggcagcgggt tgagccgatg cgatttcttt gtgaccaaaa actatgaaat ctacataaac
     2581 gaagtcaata ctttgcctgg cttcacttcc cgcagcatgt atccgttact ttgggaagaa
     2641 acgggattga aatacagcga cttggtggaa gagctgatcc agttggcctt gaagcgccat
     2701 gaagaaaaat tatcctatca atttgtagaa tgatgaacaa aaggccgtga ttttcctgat
     2761 cgtcagcagc actgaaagca tgtactgctt gctgcgaacc cgggaaagga cggctttttt
     2821 tgagagcggc cgaaggaact gccgaaaaaa acggaaatga ggaactgagt atgaaaccat
     2881 tacgcatcat tgatgttgtg aaagctgtcg gagcgatcga ctatacgtcg gccaaccgct
     2941 tcgctgaaat aaattccgta gtcttcgact cccgaaaagc aacgtccgat agtttgtttg
     3001 tgcctttgca gggggaaacg gatgggcatg attatgtcca atcggcaatc aaaaacgggg
     3061 cgacggcaac tttatggagt cgtccagcag gagaagcgcc agaagagatt gccgtcattc
     3121 tggtggatga tacgctggaa gctttgcaaa aactggcgga agcctatctg aatatgatcc
     3181 aaccaaaagt ggtcgcgatc accggcagca acgggaaaac gaccactaag gacatgacgg
     3241 ctgccatcct gtccgcgcgt tttcgtgtcc acaagacgga aggcaattac aataatgaaa
     3301 tcggtatgcc gatgaccatt ctggagatgc cggaagacac ccaagtgctg gtgcttgaga
     3361 tgggcatgag caattttggc gaaatcagtt tgttgagccg attggcaaaa cccgatatcg
     3421 ccatcatcac cttgatcggg gacagtcatc tggagttcct cgggagccgc cttggcatcg
     3481 ccaaagccaa aatggaaatc ttggaaggat tgaaaccgga agggacattc atctatcctg
     3541 gcgacgagcc gttgattgcc gacgagctgg ctgaggaatc ccacttccgt caactgactt
     3601 tcggtacaga tgaaacggcg gccgtgtatg cctatgacat cgttccaggc aaaaccagaa
     3661 caacatttca cgtgaatctg gatccgagcg tcgatctgga gatccctgta ctgggtgtgt
     3721 acaatgtccg gaatgctttg gcagccttag ccgctgcgca ggtattggac atgccgcttg
     3781 ctgaagtgaa agaagctttg gcgaccttcc agctgaccgc caaccgtacg cagtggctgg
     3841 atggcgtaaa tggctcgcag ttgttgaatg atgcgtataa tgctagccct acttcgatga
     3901 cggctgtgct gcgctcattc gcgaagctcc cgcgggaggg aaagaaaatt gcagtgctcg
     3961 gcgatatccg tgaactgggc gaacgttcag ctgagctgca tgcaggattg gcagccgaaa
     4021 tttcccctga agagtttgat caggtctatc tttacggccc ggaaatgggc gccctttatg
     4081 aaaatttaag gactgtcttc ccggaaggca cgctttatca ttatgttggg gagaaaacac
     4141 cactggtcga aaagttgaag tcagagcttc aaaaagccga ccaagtgctg attaaatcca
     4201 gtttcggcac tgatttattg gctgttgtag cagcgttgcg tgtataatag gaaatataaa
     4261 ggatttggat gggctacgtc gtcgcgaaag caaacagacc ccttttaaag ccaatctgga
     4321 acgcatatgt caaatgtcgg taaattgtag aattacttgt cattttcatt gaaaatatac
     4381 ttacaacaag gtttttttgt aaggaacttc attattttaa gaggaaatga tttgtaataa
     4441 tctacggaac gtgttattat tgctgttggt aagaatagag aaataagagg gttttttctg
     4501 cgaagtgcag aatgtttgat ttatcaagct ttctattcaa gggattaagt aaaaaaggtt
     4561 tatcttttat ttataagatt atgaaccgca tgagatatgt gggtaaaaga aaagactctc
     4621 taaattttag tttctcaaaa ttaggaggaa acatgaaatt ttcagaatta gggttaaacg
     4681 cggagttatt gaagtcgatt gagcaaatgg gatttgagga agcaacaccg atccaagaac
     4741 aaaccattcc tttggcattg caagggttgg acgttattgg gcaagcacaa acaggtactg
     4801 gtaaaacagc tgctttcgga ttgcctatgc tgcaaaaaat cgataacaac aacaaagtta
     4861 ttcaaggtat cgttattgca cctacacgtg agttggctat ccaaacgcaa gaagaattat
     4921 accgtttgag ccgcgacaaa aaagtatccg tgcaggttgt ctatggtgga gctgacatca
     4981 accgtcaaat ccgtgctttg aaaaacaaac cgcaaattat tgtcggaaca ccaggtcgtc
     5041 ttttggacca catcaaacgc aaaacaatcc gtctggatca catcgaaaca ttagttctgg
     5101 atgaagcaga tgaaatgctg aacatgggct tcttggaaga catcgaagca atcttgagcg
     5161 aaacaccggc tgaacgtcaa acattgttgt tctcagctac aatgcctgac tcaatcaaaa
     5221 aaatcggtgt caaattcatg cacgctccag agcatgtcaa aatcaaagcg tcacacactg
     5281 cagctaactt aatcgatcag tatttcgtta aatgtaaaga ctttgaaaaa ttcgatacaa
     5341 tgactcgttt gttcgatgtg caaaatcctg aattggctat cgtattcggc cgtacaaaac
     5401 gtcgtgttga cgaattgtca aaaggtttgg aattacgcgg ttaccgtgca gaaggaatcc
     5461 acggcgacct gacgcaacaa aaacgtatga gcgtattgaa cgcattcaaa acaggcgatt
     5521 tggatatctt ggtagcgact gacgttgctg ctcgtggatt ggatatttca ggtgttacac
     5581 acgtatacaa ctacgatatc ccgcaagatc cagagagcta tgttcaccgt atcggccgta
     5641 ctggtcgtgc cggtaaagaa ggtatgtcag tgactttcgt tacaccaaac gaaatggatt
     5701 acttgcgtac catcgaaaac ctgaccaaaa aaccgatcac tccattacaa ccaccgagtg
     5761 atgaagaagc tttccgtggc caagtgaaaa cagctatgga tgaagtggaa acattggtta
     5821 aagaaacaga aacagacaaa tacaaacgtg cagctgttcg cttgcttgaa gaacacactg
     5881 ctgaagattt ggtggcagca ttcttgaaga gtctttctaa agatgctact gatgttcctg
     5941 ttaaaatcac accagaacgt ccattgcctt caagaaaagg taaaggcggc ggtggttaca
     6001 aaggtaaagg tggcggcggc tacaaaggta accgtagttc atcagacaga cgtggcggcg
     6061 gagaacgcgg cggtgagcgc agtttcaaca aaaatcgctc aagcgacagc aaaccacgct
     6121 ccggcggcaa acgctatgat gacaaacgtg gtggcggcag cagtgccagc gcacctaaaa
     6181 aaagcagcca tagcttcact atccgtaaca acaacgacta atcagtgata aaacctcttc
     6241 tccaattggc ttccgccagg agaagaggtt tttcttatgc tggaattttt ttttgttttt
     6301 ggtaaaatga gaacaatgca tgaaaaagag gcggtgaatg gaatgattta tggaatagga
     6361 ttggacgtga ccgagctgga ccgcatcaca agtgcctatg agcggcggtc agattttgcg
     6421 gaaagggttt tgaccgaagc ggaactgaaa ttgtttctgg ttttgagcgg gtcacgccaa
     6481 atggaatttc tggctggacg ctatgccgca aaagaggcct tctcgaaggc ttacggaaca
     6541 ggtatcggca aattgtcttt ccaagatatc gaaattttgc ccggagagta ccggaagcct
     6601 gaaataacca aatctccgtt cgaagggaaa gggttcgttt ccatcaccca ttcggggaac
     6661 atcattgccg cgcaagtggt actagaaaag taaaggggtc ttacaacatg gtagtaggaa
     6721 agcaccgtcc cacagtggaa accatcgatt tggaagctat ttcgtggaac atccaacaaa
     6781 tcaggaagag tctgtctcct gaaaagaaat tttttgcggt cgtcaaagcg gacgcctacg
     6841 gactgggcgc tgtcccggtc gcgaaaaagg caaaggaagc aggcgtggac ggtttttgtg
     6901 tcgcgctgtt ggatgaggca atggaattgc gggaaaacgg actgtccgat gacttcatta
     6961 tggtcgtggg cccaactgag tcagaagacg ctgcgctcat agctgataac ggaatcagcg
     7021 ttgccgtaac ggcaaaggaa tggttggatg acgccttacc gtatctgaac agacggaaaa
     7081 cggagctgcc gattcgggtg catttggcca tcgatacggg gatgggacgg attggcttgc
     7141 ggacggtcga ggaggtcaaa gcctttgagg cgcgtattgc tgaactggat gattttgttt
     7201 tcgaaggtat ctttacgcac tttgcgacgg ctgatggtga agatccgacc ttggttgatc
     7261 gccaactcgc tttgtttgaa gacatcttga acgcattggc tcagcgaccg attgtgcatc
     7321 tggcaaattc cgccatttcc ctctggcatg aagcattcca gacggatgcg gtccgcgtcg
     7381 gtatcgcaat gtacggatac aatccttctg acaccgtatt ggagcttccg tatgaattga
     7441 agccatcggt acagctggat acgcacatca gctatgtcaa gcagatgcat gaaggcgaca
     7501 caatcgcata tggcgcccgt taccgcgctt ttgaaggaga atggatcgct acgctcccaa
     7561 tcggctatgc ggacggatgg cggcgggatc ttcgccatca gacggttatc gtcgatgggc
     7621 agcgctgccc aatccgggga gtcatctgca tggatcagtg catgatcagt ctgccgaaag
     7681 aatatccaat tgggacgaag gtgacgctct tgggcgaaaa cggtgggctt gtcaacaatc
     7741 catccgaaat ggccgtaacg attgatacga tcggatatga aattttgacg gggatcaacg
     7801 cccgcgttcc gcgtatctac aaatagaagg aaagataaat ctggaaagct gtctgttgcc
     7861 tgttaggggc agacagcttt ttttgctgtg tgtgtgatag aaacgaatgt gggggagaga
     7921 gggcgaggga gagggcgctc caactccggc taagagttgt tccgccggag aagagcggct
     7981 ggactgtgtt ccacctccag ctagggttgc ctcgccggag aagagcagct cgatcgcatt
     8041 ccacctccgt ccagaagcgc ttcaccggag gagaacagtc atacaggatc atacagagtc
     8101 atacataatc cgcatctctg gcgctctcgc ctttaggtaa tgaaaatcag atgaaaatac
     8161 gtcgccagtc gttttcaaaa gcgaacatgt atggtaaact gtcctagctc aaagatgagt
     8221 gcgtggttcg tctaccatcc cacgaaaaaa aactaggagg tttttaggtt gaatacaaga
     8281 aaaatggtta tcaatacaat tattgcagcg ctatatgttg ccatgacagg gatttttgca
     8341 tttatgtcat tcggtgccgt ccaattccgt gtttcagaaa tgctgaatca tttggtgggc
     8401 taccacaaaa attataaata cggtgttttg gcgggtgttt ttatcgccaa tctgattttt
     8461 tcgacgttag gagcttggga cttggtgttc ggatttggcc agacattgat ctctttcctt
     8521 attttggaaa aattattcaa acccaacgat tctgaaatga agcgcatgat gatgaccgcg
     8581 ttcgtcttca cgatgacgat ggtccttgtc gcgatcgaac tctatatcgt tcttgatttg
     8641 cctttctggt tcagttttgc gacagcggca ttcggggaag cggtcgtgct ggttgtgtcg
     8701 gcgccgctca tgaaatacct gaacaaaatc atcggttttg ccgagaaaat ggcttgatcc
     8761 gttaacattt agtatgagaa aaactgggga acggaggctt ttgctggatg gtcagcagat
     8821 cctcctgttt tttttgctag gatatttctc atcattctct tataatagag aggtattcga
     8881 aaaagaggga ggaaataata tatggaaatg tacgcaatca cagtcggtcc tatccaagaa
     8941 aattgttatt ttttgatcgg agaccatgaa aatgacacga tcatctttga tccgggtgag
     9001 caagctgagg acatcatcgc agccatcgaa gagaatgcgt tgcatccgat agcgatcgtg
     9061 ttgacccatg cgcactacga tcatatcgga gccctcgaaa cgatccggga aaaatacgac
     9121 gttcccgttt accaaaatcc gattgaacgg gaatggctgt tggatccgca actgaacggg
     9181 tccgcgcgca atccgaacgt tcccgatgtc cgattggcaa aagaagcgga tgtcttcttc
     9241 gaggagatgg gcccttacga aatcggcgaa ttccgttttg agatgcaaca tattcctgga
     9301 cattcaccag gcagtactgt ttttattttc cgcgagaacg ggttcgcgat cgttggggat
     9361 gtcatcttca aaggcagcgt aggccgcagc gatttgccgg gcggcagcca tgaaacactg
     9421 atcgagggca tccagaaata tatcgtaacc ttgccagggg aaacggtcct gttcccggga
     9481 cacggcgatc ctacgacagt gagggaagaa atctattcaa acccttatct gaatggcgct
     9541 acgcgctgat ccgggtatgc gcaaaaaagt cggacaagca caaggcttcg tccgactttt
     9601 tgtattagtt tttagatcga gcgggcgtct agagcctcag cggaattccc gatgagggcc
     9661 tcctcgccgg gaaaacgcca ctccgggcac tcaatgtaat gtaatgtgat gggttggatt
     9721 tcagaaatcc acttgcaggt tttcgatttc cgtcacgcgg aaatccttac gctccagcgc
     9781 cttgacgatg gagtccagca cctgtttggg cgtgccggcg ggtaatgtga ccaataccct
     9841 gcgcaaagcg tcctcacggt tgatgtccag ggaaatgcag ctggcgatgg aggaatagcg
     9901 gttgatgatt ttagtgatat ggtacagatc gcctttttga ccgggcgcga tgacggtcag
     9961 cacatagctt ccgacatcga tgctccatga ttgggccagc atattcagta aggcattatg
    10021 ggtcaggatg ccgtaaaagg tcatatcctt attcaaaacg gaaatatagg gcaattcttt
    10081 gatggtgaaa aataccttaa agaatgagct gtctatcgaa atgaattttg tggcattctt
    10141 cagcaagtgc gtaacgggca ggttcatttc gccgccgttc gctttgtgcc gatagatatg
    10201 catcttgtag atgtttccac gaaaaatggt ttctgtttcg tccagaatcg gaacacaacg
    10261 gaagccggac tcttcgagga tatccagtgc atcctgaagc gtggctgact ctttgacgat
    10321 ggtcaactcc ctttttggga tggcaagtga gcggataagc atattattaa aatcctccta
    10381 aaaacgcggt tgtctaatgt gataataaca ttaaaatcaa aaaaagcaac ggttaaaaac
    10441 acatagttgg aaattcttaa acatttgata aatcaagcag ggaagc
//
LOCUS       DAOMUJ010000015        10265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1592_length_10265_cov_2.287561, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000015 DAOMUJ010000000
VERSION     DAOMUJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_10265_cov_2.287561"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /gene="secY"
                     /locus_tag="PLT73_00895"
     CDS             <1..946
                     /gene="secY"
                     /locus_tag="PLT73_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRM18969.1"
                     /translation="TVILAFVQSVGLSIGFNQLSAFGLVNDPGTTTYMIIALVMTAGS
                     MLVVFIGESISMHGIGNGTSLIIFSGIIARIPSDLYTYYNDRFVDAGSDLMNNILFSV
                     ALLIAILLVVILVVYVQQAERKIPIQYSKRASGSNQSAHLPLKINSAGVIPVIFASSF
                     MMTPQTILGFFAASQSEASWYQILSTIFNYREPIGATIYTVLIVVFTYFYAFIQINPE
                     KMAENLQKQGGYIPSVRPGKGTEDYISGMIMRLSTVGALFLGLIALLPIIASGLWDLP
                     DSLALGGTSLLIVVGTALETARQIEGRMVKRNYQGFIQ"
     gene            1014..1658
                     /locus_tag="PLT73_00900"
     CDS             1014..1658
                     /locus_tag="PLT73_00900"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRM18970.1"
                     /translation="MNLIIMGLPGAGKGTQAEKIIAKYNIPHISTGDMFRAAMKNETA
                     LGLEAKSYMDKGELVPDEVTNGIVKERLAESDTEKGFLLDGFPRTLVQAKALEAIMNE
                     LDKKIDAVINIDVNPEVLMQRLTGRIICRSCGATYHKTNNPPKIEGTCDRCGGHEFYQ
                     REDDKPETVENRIQINLEQSQPIIAFYSEKGLLHNVDGEIGIDKLFTEIQKIIE"
     gene            1794..2012
                     /gene="infA"
                     /locus_tag="PLT73_00905"
     CDS             1794..2012
                     /gene="infA"
                     /locus_tag="PLT73_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM18971.1"
                     /translation="MAKDDVIEIEGVVVETLPNAMFKVELENGHIVLAHVSGKIRMHY
                     IRILPGDKVTVELSPYDLTRGRITYRFK"
     gene            2046..2159
                     /gene="rpmJ"
                     /locus_tag="PLT73_00910"
     CDS             2046..2159
                     /gene="rpmJ"
                     /locus_tag="PLT73_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRM18972.1"
                     /translation="MKVRASVKPICEKCKVIRRNGRVMVICENPKHKQRQG"
     gene            2180..2545
                     /gene="rpsM"
                     /locus_tag="PLT73_00915"
     CDS             2180..2545
                     /gene="rpsM"
                     /locus_tag="PLT73_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRM18973.1"
                     /translation="MARIAGVDVPRDKRVVISLTYIFGIGLNTAQKVLAAAEVSEDTR
                     VRDLTNDELDRIRIEVDKLKVEGDLRREVNLNIKRLIEIGSYRGMRHRRGLPVRGQNT
                     KNNARTRKGPAKSIAGKKK"
     gene            2573..2959
                     /gene="rpsK"
                     /locus_tag="PLT73_00920"
     CDS             2573..2959
                     /gene="rpsK"
                     /locus_tag="PLT73_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRM18974.1"
                     /translation="MAKKVARKRRVKKNVEAGVAHIRSTFNNTIVMITDVHGNAISWS
                     SAGSLGFRGSRKSTPYAAQMAAEAAAKVSMEHGMKTVEVAVKGPGSGREAAIRSLQAA
                     GLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            3041..3985
                     /locus_tag="PLT73_00925"
     CDS             3041..3985
                     /locus_tag="PLT73_00925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRM18975.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGTAVTTIQIDGVLHEFSTIDGVVEDVTQIILNIKKLALKLYTDEDKTIEIDVK
                     GPAVVTAADITHDSDVEILNPDLYICTVSEGAHFHVRLDVKNGRGYVRSEYNKTEDMP
                     IGVLPVDSIYTPISKVNYQVENTRIGQKNIYDKLTLDVWTDGSISPEEAVSLAAKILT
                     EHLNIFVNLTDEARKAEIMVEKEETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQE
                     LTDKSEAEMIKVRNLGRKSLEEVKNKLNDLNLGLRQDD"
     gene            4012..4392
                     /gene="rplQ"
                     /locus_tag="PLT73_00930"
     CDS             4012..4392
                     /gene="rplQ"
                     /locus_tag="PLT73_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRM18976.1"
                     /translation="MAYRKLGRTSAQRKAMLRDLTTDLIINERITTTEARAKEIRSTT
                     EKMITLGKRGDLAARRLAAAYVRNEVADVREEDDKIVVQSALQKLFSDIAPRYAERQG
                     GYTRILKTEPRRGDAAQMVIIELV"
     gene            4788..5627
                     /locus_tag="PLT73_00935"
     CDS             4788..5627
                     /locus_tag="PLT73_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM18977.1"
                     /translation="MNEIIELRNVTFSYSEEDARPALNNVSLTIQQGEWIAIIGPNGS
                     GKSTLAKTINGLIEANSGEVIIEGVALNAETVWDVRKKIGMVFQNPDNQFVGSTVQDD
                     VAFGLENVGILREEMVKRVADAVAAVNMANFMDKEPARLSGGQKQRVAIAGIVALSPD
                     IIILDEATTMLDPEGRHEVIETIQEIKEKENLTVISITHDIDEAAKANRIFVMEAGQL
                     TRIGTPEEIFSLGKEIIDIGLDIPFPEKLKYQLKRQGLEVPENYLTEEGMVNWLWTLL
                     SKK"
     gene            5603..6514
                     /locus_tag="PLT73_00940"
     CDS             5603..6514
                     /locus_tag="PLT73_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM18978.1"
                     /translation="MDITFEKVGYSYSAGTPFESRALYDVNLNIPDGSYTALIGHTGS
                     GKSTVTQHLNALLKPTEGSVTIGARIITNKSNNKNLKDLRKKVGIVFQFPESQLFEET
                     IAKDIAFGPMNFGVSEEDAMAIVKRVLPLVGLDESFMDRSPFDLSGGQMRRVAIAGVL
                     AMEPEVLVLDEPTAGLDPAGRREIMNMFNQLHIDKGLTIVLVTHQMNDVATYADHVVI
                     LEKGTVVKMGQPDEIFQDAQWLQEKQLGLPDALAFLDRLSKRTGVDFGDERPLRTEQL
                     AAVLIAKINENKGSKNAAKEEGGNHVG"
     gene            6504..7301
                     /locus_tag="PLT73_00945"
     CDS             6504..7301
                     /locus_tag="PLT73_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM18979.1"
                     /translation="MLDKLLFGRYIQGSSLVHRLDPRAKLIGAFYFIIVIFLANNWQT
                     YLIMTLFTFLCVILSDIKLSVFLNGVKPLIWLILFTVLLQILFTRGGETYLVLGPISI
                     TSFGVINGAFIFMRFVLIIFISTLLTLTTMPLSLTDAIEKLLGPLKRFKVPVHEIALM
                     LSIALRFVPTLMDEASKIMNAQRARGVEFGEGNIVKQMKAVTPILVPLFVSSFNRADE
                     LANAMEARGYQGGEGRTKYRILDWQKRDTLSMLAFVALTGLLLFFRS"
     gene            7426..8199
                     /gene="truA"
                     /locus_tag="PLT73_00950"
     CDS             7426..8199
                     /gene="truA"
                     /locus_tag="PLT73_00950"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM18980.1"
                     /translation="MTAQRYKCIVQYDGTSYVGYQVQPNGNSVQTEIEKALKKMSNGQ
                     IIPIQASGRTDSGVHALGQVIHFDYPAAIKPDHLLRALNSLLPDDILIKSVEHAAEDF
                     HSRYHALGKKYIYRVDLDRFPNPFKRLYTTHHPYRFNMENLEQAIKKLEGEHDFTSFC
                     STKTDKTDLVRTVYEASVRKDEVNNELVFTFRGNGFLYNMIRIFVGTLLQIADGLKKV
                     EEIDRLLEVKDRRKAGPTAPSQGLYLVEVYYDEEKLRNG"
     gene            8211..8477
                     /locus_tag="PLT73_00955"
     CDS             8211..8477
                     /locus_tag="PLT73_00955"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRM18981.1"
                     /translation="MFEKERAEIDIIDRELVKLFERRMDAVTEIARIKKEHKLPILDQ
                     SREDKVLDKVRRLTENKAYEDSVEDLFRSLMTITKAFETKQNEE"
     gene            complement(8574..9686)
                     /locus_tag="PLT73_00960"
     CDS             complement(8574..9686)
                     /locus_tag="PLT73_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM18982.1"
                     /translation="MESISIQSYLAQLGNRKDPHTGAVSAPIYLSTAYGHPGLGQSTG
                     YDYTRTANPTRDILQEGLAVLENGVQGFATSSGMSAIQLAFSIFPANSHFVASRDLYG
                     GSFRYFQDMEKKGFYSFTYVDDPIDITSAIQDNTAAVYIETPTNPLMKETDIAAVAEI
                     AKKHGLLVIVDNTFYTPLLQRPLDLGADIVVHSATKYLGGHNDLLAGAVVTNNKALGE
                     QLAFQLNTAGGTLDAFDCWLLVRGMKTLPLRMKQHQANAQAVVAYLESEKLIKSVYYP
                     GKGGMLSFTLHDKATIPVVLDALNIFTFAESLGGVESLITYPTTQTHADIPIEVRESY
                     GLTDDLLRISTGIEDADDLIADLRQAFAKAAVTAIV"
     gene            9932..>10265
                     /locus_tag="PLT73_00965"
     CDS             9932..>10265
                     /locus_tag="PLT73_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18983.1"
                     /translation="MVTFTKELKRIPRGDVPDFVAAAMPQFYEAIGCPNDVILSVQAS
                     MAHYSTPKKNVPVEEYEAFEVTLTKKGAFVAVEDIVKDHAIIEAFKPYKTSGKGAYPF
                     VPAEVIEQL"
BASE COUNT         3017 a         2116 c         2483 g         2649 t
ORIGIN      
        1 gacagtcatt ttagcatttg tacaatctgt cggtctatcg atcggtttca accaactctc
       61 agcatttggg ctggtaaatg atccggggac gacaacttat atgatcatcg cgttagtgat
      121 gacggcaggt tcgatgcttg ttgtttttat cggagaatcg atttcaatgc atgggatcgg
      181 aaacggaacc tcattgatca ttttctctgg tatcatcgca agaatccctt cagatcttta
      241 cacttactat aatgatcgtt ttgtggatgc aggatccgat ttgatgaaca atattttgtt
      301 ctcggtcgcg ctattgatag caatattatt ggtggtcata ctcgttgtgt acgttcaaca
      361 agctgaacgt aaaatcccca ttcaatattc caagcgtgct tccggttcga atcaatccgc
      421 gcatttgcct ttgaaaatca attcagctgg agtcattcca gtaatctttg ccagttcttt
      481 catgatgaca ccgcagacga tccttggatt tttcgcggca agtcaaagtg aagccagctg
      541 gtatcaaatt ttgtcaacca tctttaatta ccgtgaaccc atcggtgcaa caatctatac
      601 ggtattgatc gttgtcttca cttacttcta tgccttcatt cagattaacc ctgaaaaaat
      661 ggcagagaac ttgcaaaagc aaggcggcta tattccaagt gtacgcccgg gtaaagggac
      721 agaagattac atttccggta tgattatgcg attaagtacg gtcggcgcgc tatttcttgg
      781 actgattgcg ctattgccaa tcattgcctc aggtttgtgg gatttgccgg actcgctcgc
      841 ccttggcggt acaagtcttc tgattgtagt tggtactgca ttggaaacgg caagacaaat
      901 tgaaggtcga atggttaaac gtaattacca aggatttatt caatagtaag tttgtgaggg
      961 ggaaagtgat ttcctcgtca catatgctta catctttagg aggcagtaaa atgatgaacc
     1021 taattattat gggtcttccc ggtgctggaa aaggaacaca ggctgaaaag atcatcgcga
     1081 aatacaatat tccgcatatc tctacagggg acatgttccg agctgctatg aaaaatgaga
     1141 cagccttggg cctggaagca aaatcatata tggataaagg cgaacttgtt ccagatgaag
     1201 ttacaaacgg aatcgttaaa gagagattag cagaatctga tactgaaaaa ggctttttgt
     1261 tggatggttt tccaagaacc ctcgtacaag caaaagcact tgaagctatc atgaatgaac
     1321 tcgacaaaaa aatcgatgct gttattaaca ttgatgtgaa ccctgaggtt ttgatgcaac
     1381 gtctaacggg tagaatcatt tgccgctctt gcggagcgac ttaccacaaa acaaacaatc
     1441 ctcctaaaat agaaggaacg tgcgatcgtt gtggcggaca cgaattctat caacgcgaag
     1501 atgataaacc tgaaaccgta gaaaatcgca ttcagattaa tcttgaacag tctcaaccaa
     1561 tcattgcatt ttacagcgaa aaaggattgt tgcacaatgt ggatggcgaa atcggcatcg
     1621 acaagttgtt tactgaaatc caaaagatta tagaatagtt gcaaatgtca tttgaagtta
     1681 atcgtaaatt tcttttcaat agatacttgc tatggtataa tctagcagtt agagtaaaaa
     1741 ttgccaagac atgagattaa tcagctcaac agaatgttag gaggttagca ggcgtggcga
     1801 aagacgatgt cattgagatc gaaggagttg tcgttgaaac tttgcccaat gcaatgttta
     1861 aagtcgaact tgaaaatggt cacatagtat tagctcacgt atctggtaaa attagaatgc
     1921 attacatccg cattttgcca ggcgataaag ttacggtaga attatctccg tacgatctta
     1981 ctcgtggacg cattacttac cgctttaaat aattgcactc catcactaaa taggagggaa
     2041 tttaaatgaa agttagagca tcagtaaaac ccatttgcga aaaatgcaaa gtcattcgcc
     2101 gcaatggtcg tgttatggtg atttgcgaaa atcccaaaca caaacaacgc caaggataat
     2161 aacaaggagg tgcctataaa tggctcgtat cgcaggagta gatgttccgc gtgacaaacg
     2221 tgtagttatt tcattaactt atatttttgg tatcggatta aacacagctc aaaaagtttt
     2281 agcagcagct gaggtttcag aagatactcg tgttcgtgat ttaacgaatg atgaattaga
     2341 ccgtatccgt attgaagtgg ataaattaaa ggttgaaggt gatcttcgtc gtgaagtaaa
     2401 cctaaatatt aaacgattga tcgaaatcgg atcttacaga ggaatgcgtc accgtcgtgg
     2461 tttgcctgtt cgcggacaaa acacaaagaa caatgcacgt actcgtaaag gcccagctaa
     2521 atcaatcgct ggtaagaaaa aataataatt aagtgaagga ggtcaaaact tcatggctaa
     2581 aaaagtagct cgcaaacgcc gcgtaaaaaa gaatgttgaa gctggtgtag cacatatccg
     2641 ctcaactttt aataatacta ttgttatgat tacagatgtt catggaaatg ctatttcatg
     2701 gtcatcagca ggttcattag gattcagagg ttctcgtaaa tctactcctt atgctgcaca
     2761 aatggctgca gaagcagcag ctaaagtttc tatggaacat ggcatgaaaa cagtcgaagt
     2821 agctgttaaa ggtccaggat ctggacgtga agctgctatt cgttcattac aagctgcagg
     2881 attggaagtt accgcaatcc gcgatgtaac gcccgttcct cataatggat gccgtccacc
     2941 aaaacgtcgt cgtgtttaat tgagaattgt actgttatgt aacgaaacgc aatggactca
     3001 ctaacatgaa cttcacgttt tgaaaggggt aaattgataa atgatcgaaa ttgaaaaacc
     3061 aagaattgaa acgattgaga tcagcgaaga tgccaaattc ggtaaattcg ttgtagaacc
     3121 actagaacgc ggttatggaa caacacttgg gaactcatta cgtcgaattc tattatcttc
     3181 acttcctggt acagctgtaa caactatcca aatcgatggt gttctacatg aattttcaac
     3241 gattgatggt gttgtcgagg atgttaccca aatcattctg aacatcaaaa aattagcttt
     3301 gaaattgtat acggacgaag acaaaacaat cgagattgac gtaaaaggtc cagcagtcgt
     3361 gactgcagcg gatattaccc atgacagcga tgtggaaatc ttaaaccctg atttatatat
     3421 atgtacagtt tcagagggag cacattttca tgttcgtttg gatgtaaaaa atggccgtgg
     3481 ctatgttcgt tccgaatata acaaaacgga agatatgccg atcggtgttt tgccagtcga
     3541 ctccatttat actccaatca gcaaggtgaa ttatcaggtt gaaaatactc gcatcggaca
     3601 aaaaaatatt tacgataaat taacgttgga tgtctggaca gatggttcca tcagcccaga
     3661 agaagcagtc agcttggctg ccaaaatttt aacggaacat ttgaatattt ttgttaatct
     3721 gaccgatgaa gcccgcaagg ccgagataat ggttgaaaaa gaagaaacac ataaagagaa
     3781 aatgctggaa atgactatcg aagaattgga tttatccgta cgttcttata actgtttgaa
     3841 acgcgcaggc atcaatacag ttcaagaatt gacagacaag tctgaagcag agatgatcaa
     3901 agtacgtaat ctgggacgca aatcacttga agaagtgaag aacaagctga atgatcttaa
     3961 tctaggcttg cgtcaagacg actagtactg cacaaaggag gaaaacctag gatggcttac
     4021 cgtaaattag gacgcacaag cgctcaaaga aaagcaatgc ttcgtgattt gactactgat
     4081 ttaatcatca acgaacgcat tacaacaact gaagcacgtg ctaaagaaat tcgttcaact
     4141 actgaaaaaa tgattacttt aggcaaacgt ggagatttag ctgctcgtcg tttggctgca
     4201 gcatacgttc gtaacgaagt agcagatgtt cgtgaagaag atgataaaat cgttgttcaa
     4261 tcagcgttac aaaaattatt ctctgatatc gcacctcgct atgcagaacg tcaaggcgga
     4321 tacactcgta tcttgaagac tgaacctcgc cgtggcgatg cagcacaaat ggttatcatt
     4381 gaattagtat aattcaatcg cataaagatg aaacgttcac ttttagatga aatagagtgt
     4441 tatgatgatg ggtctgctta ttgcttccct agtctagctc aaggttcttc cgaagatcgc
     4501 atgattttcg gaagggcatt ttttcatcaa aaaaagtgcc tttttttatc aaaagaaact
     4561 agaaaatgcc aatatatttt ctagtttctt ttgatatata aataaaagcg ttttatttcc
     4621 ttgctgtgtc aagacagtgg atgaaaaata cgggagagta gtttatactt actaaagtag
     4681 acatttcctt gccgtatttt tcatcttttt ttatacccgc ttgtctgcgg gcggatgatc
     4741 ggatacggcc ttatagattc aataaaagag aaagtaggac tgcaatcatg aatgaaatta
     4801 tcgagttgcg caatgtgact ttttcctatt cagaggaaga tgcaagaccg gcattgaaca
     4861 acgtttcgtt gacaatccag caaggtgaat ggattgccat catcgggcct aatggttcag
     4921 gcaagtcaac tttggccaaa acgatcaatg gcctgataga agcgaactcc ggagaagtca
     4981 tcatcgaagg agtagctctg aatgcagaga cggtttggga tgtccgtaag aaaatcggaa
     5041 tggtattcca gaatccggac aatcagtttg tcggttcaac agtgcaggac gacgttgctt
     5101 tcggcttgga gaatgtcggc attctccgtg aggaaatggt caagcgggta gccgatgcgg
     5161 ttgcggctgt caatatggcc aacttcatgg acaaggaacc ggcaagactt tcgggcgggc
     5221 agaagcaacg ggtggcgatt gctggaatcg tggctttgtc tcctgatatc atcatcctcg
     5281 atgaggcaac gaccatgctg gatccggaag gccgtcatga agtcatcgag accatccagg
     5341 aaataaaaga aaaagaaaat ctgacggtga tctcgatcac ccatgacatt gatgaggctg
     5401 caaaagcgaa tcgcatcttt gtgatggagg ctgggcaatt gacacggatc ggcacgccgg
     5461 aagaaatatt cagtttgggc aaggaaatca tcgatatcgg tttggatata ccgttcccag
     5521 aaaaactgaa ataccaattg aagagacagg gactggaagt tcctgaaaac tatttgactg
     5581 aggaagggat ggtgaactgg ttatggacat tactttcgaa aaagtaggct actcgtattc
     5641 agccggaacg cctttcgaaa gccgggctct ttatgatgtg aacctgaata tcccggacgg
     5701 cagctatacg gcattgatcg ggcataccgg cagcggaaaa tcaacagtca cgcaacatct
     5761 gaatgcttta ttgaaaccca ctgaaggcag tgtgacgatc ggagcccgca tcataacgaa
     5821 caaatcgaac aacaaaaatc tgaaggacct ccggaaaaag gtagggattg tgttccagtt
     5881 tccggagtcg caactgttcg aggagacgat cgctaaggat atcgccttcg gaccgatgaa
     5941 cttcggcgtc agcgaagagg atgcaatggc gattgtgaag cgcgtattgc ctttggttgg
     6001 cttggatgaa tccttcatgg atcgttcccc attcgacctc tcagggggtc aaatgagacg
     6061 cgtagcgata gccggcgtat tggccatgga gccggaagtg ctggttctgg atgaaccaac
     6121 ggcaggattg gatcctgcgg gcagacggga aatcatgaat atgttcaatc aactccatat
     6181 cgacaaaggt ctgacgattg tattggtcac gcatcagatg aatgatgtcg cgacttatgc
     6241 ggatcatgtt gtcattctcg aaaaagggac agtcgtcaaa atgggacagc ccgatgagat
     6301 tttccaggat gcccaatggc tgcaagaaaa gcagttgggc ttaccggatg cgctagcatt
     6361 tctggatagg ttgagtaaac ggaccggagt agacttcggc gacgaaagac cgcttcgtac
     6421 cgaacagctg gcggctgtgt tgatcgctaa aatcaacgaa aataaaggct ccaaaaatgc
     6481 cgctaaggaa gagggtggca atcatgttgg ataaattact gttcgggcgc tatatccaag
     6541 gcagctcgtt ggtccataga ctggatccgc gcgcaaagct gatcggggct ttctatttca
     6601 tcatcgtcat cttcctggcc aacaactggc agacttattt gatcatgacg ttgttcactt
     6661 tcttgtgcgt tattctgtct gatattaaac tgtcagtctt tctgaacggc gtgaaaccgc
     6721 tgatttggtt gatcttgttc acggtattgc tgcagatcct cttcacaagg ggaggggaaa
     6781 cgtacttagt gctgggaccg atcagcatca cctcattcgg ggtcatcaat ggtgccttca
     6841 tcttcatgcg tttcgttctc atcatcttca tttcgacgct gctgacgctc acgacgatgc
     6901 cgttgtcgtt gaccgatgcg attgaaaagc tgttgggacc attgaaacga tttaaagttc
     6961 ctgtccatga aattgcgctg atgctttcca tcgcgttgcg ttttgtgccg acgctgatgg
     7021 atgaggcttc aaagatcatg aatgcacaac gggcccgcgg tgtcgaattc ggcgaaggca
     7081 atatcgtcaa gcagatgaag gccgtcacac cgattttggt gccgctcttc gtcagctcct
     7141 tcaaccgggc tgatgaattg gcaaatgcca tggaggcacg tggctatcaa ggcggagaag
     7201 gccggacgaa gtaccgcatc cttgattggc agaagcgcga tacgttgagt atgctggcgt
     7261 ttgtcgcatt gacagggttg ttattgttct tccgctcatg agcgcatacg ggctgattaa
     7321 gccgctagag aacgagtctg gggaccgggt agcaattccg gtccctatta aaatggaaaa
     7381 actaccacat acatcagcaa aataagaaag gggcatacgc caaccatgac tgcacaacgg
     7441 tacaaatgca tcgtgcaata tgacggaaca agttatgtgg gctatcaggt ccaacctaac
     7501 ggcaacagtg tccagacgga aatcgaaaag gcgttgaaaa aaatgtcgaa cgggcagatc
     7561 attccgatcc aagcttccgg aaggaccgat tcgggcgtcc atgcgcttgg acaggttatc
     7621 catttcgatt acccggctgc gattaagccg gatcatctgc tgcgggcgct gaacagcctg
     7681 ctgcccgatg acattttaat caaatcggtg gaacacgctg cagaggattt ccattcgcgc
     7741 taccacgcgc tagggaaaaa atatatttac cgggtcgatc ttgatcgctt cccgaatccc
     7801 ttcaaacgac tctacacgac gcaccatcct taccgcttca atatggagaa tttggagcag
     7861 gccatcaaaa aactggaagg agaacacgac ttcaccagtt tttgctcgac aaagacggat
     7921 aaaaccgatc tggtccgcac cgtctatgaa gccagcgtca ggaaagacga agtgaataac
     7981 gagctggtct tcactttcag gggtaacggc tttctctata atatgatcag aattttcgtg
     8041 gggacacttt tgcagatcgc agacggcctg aaaaaggtcg aggaaatcga ccgccttttg
     8101 gaagtgaaag accgccgcaa agccggaccg acagccccgt cgcagggatt gtatctcgtg
     8161 gaagtctact atgatgaaga aaaattaagg aacggttagg gggaaaagcg atgtttgaaa
     8221 aagaaagagc ggagatcgat ataatcgacc gggagttggt aaaactgttc gaacgccgga
     8281 tggatgcagt gacggagatc gcgcggatca agaaagagca taagctgccg attcttgacc
     8341 aatccaggga agataaggtg ttggataaag tgcgccgtct gaccgagaac aaagcctacg
     8401 aagacagcgt ggaagacctc ttccgctcct tgatgacgat cacaaaggca tttgaaacca
     8461 aacagaacga agaataaaaa agtatgctga aaaagcggct gcggaaaaga cgaaatcttt
     8521 tccgcagccg ctttttgtgc gcttgagcaa cttttatttt tcgatctatc ttctcagact
     8581 atggctgtta cagcagcctt cgcaaaggcc tggcgcagat cggcgatcaa atcatccgca
     8641 tcttcgattc cagtcgagat gcgcagcaga tcgtcggtca aaccataaga ttcgcggact
     8701 tcaatcggaa tgtcagcatg ggtctgggtg gtcggatagg taatcagact ttcgacgcca
     8761 cccaagcttt cagcgaaagt gaaaatattc agggcatcca agacgacagg gatagttgct
     8821 ttatcatgca gcgtgaaact gagcatgccg cctttacccg gataatagac cgatttgatc
     8881 agtttttccg attcgagata agcaacgact gcctgtgcat tcgcttgatg ctgcttcatg
     8941 cgcaacggca atgtcttcat tccgcggact aagagccaac agtcgaaggc atccaaagta
     9001 ccgccggccg tattcagctg gaaagccaac tgttcaccga gtgctttatt gttcgttacg
     9061 acagcccccg ccaacaggtc attatggccg ccgaggtact tggtggcgct gtggacgacg
     9121 atatcagcgc ccaaatccaa aggacgctgc aacagaggtg tgtagaaggt gttatccaca
     9181 atcaccagca gaccgtgttt tttggcgatc tcagccaccg cggcgatatc cgtttccttc
     9241 atcaacggat tcgtcggcgt ttcgatgtag actgcggccg tattatcctg gatggcggac
     9301 gtgatatcaa tcgggtcatc gacataagtg aaggaataga agcctttttt ctccatatcc
     9361 tggaaatagc ggaagctgcc gccgtaaaga tccctggatg caacaaaatg gctgttggcc
     9421 ggaaaaatgc tgaaagcaag ctggatggcg ctcatgccgg agctggtagc aaacccttga
     9481 acgccatttt caagaacagc caaaccttcc tgaaggatgt ccctagtcgg attggcggtg
     9541 cgtgtgtagt catagccggt tgattggccc agaccaggat gtccataagc tgtggacaaa
     9601 taaatcggtg cactgacggc gccggtatgc ggatcttttc tgttgcctaa ttgtgcaaga
     9661 tagctttgaa tgctgatact ttccatcgtc gattctcctt tatgcgaaat gacatacaca
     9721 agtatattgc catcctttta tcatttaaaa ttaatgttag tgcaatggta gcacgagggt
     9781 ttgaagtggt caatattttt atgtatttta agtcagtgat ggtgagatga ataatcgatc
     9841 atgcttgaac ggttatttct ctcgaatggt gttaaaatac tgaaactcgc gtataatgga
     9901 agtataagaa cgaatgaagg agtgtgtaag aatggtaacg ttcacaaagg aactcaagcg
     9961 gatcccacgc ggcgatgtgc cggattttgt ggctgctgct atgccccaat tttatgaagc
    10021 aatcggttgt ccgaacgatg tgatcctttc cgtgcaggcc agcatggcgc attactcgac
    10081 cccgaaaaag aatgtcccgg ttgaagaata tgaagctttt gaagtgacgc tcacaaaaaa
    10141 aggcgcgttc gttgcggtgg aagacatcgt gaaagaccat gctattatcg aagcattcaa
    10201 accctacaag acatccggaa aaggggccta tccgtttgtg ccggccgagg tcatcgagca
    10261 attat
//
LOCUS       DAOMUJ010000016         9414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1818_length_9414_cov_5.866011, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000016 DAOMUJ010000000
VERSION     DAOMUJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_9414_cov_5.866011"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2896)
                     /gene="gltB"
                     /locus_tag="PLT73_00970"
     CDS             complement(<1..>2896)
                     /gene="gltB"
                     /locus_tag="PLT73_00970"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRM18984.1"
                     /translation="TGTDSVGAMGIDSPLAVLSDRPQMLYLYFKQLFAQVTNPPIDGI
                     REEIVTSDTMLVGNCGNLLDPDNADTHAIYLDSPILTDSELAKIKALQDDKFKTVTLS
                     LLYRVEDDAKGMEKALERLFSAADKAIASGANILVLSDRGVNAEWAAIPALLAISGLH
                     HHLVREETRTNVGLVLESGEPREVHHFCTLVGYGCNAVNPYLTYATIRDLAEEDLLDG
                     LDAEAAEANYIHAAVHGMSKVLTKMGISTMRSYHGAQIFEALGVNSEVVAKYFTGTPT
                     RIEGIGLQEIATENRMRHDSAYKATAPYSETLGVGGHFQYSAMGEEHLYNPSTIYRLQ
                     QATRQGDYEQYKEFSREINADTNAYTLRQLMQFKPNAQDAIPIEEVESAESIVKRFKT
                     GAMSYGSISIEAHQTLAIAMNRLGGMSNSGEGGEDPRRFELDENGDSRNSAIKQVASG
                     RFGVTSHYLVNAKEIQIKMAQGAKPGEGGQLPGPKVYPEIAKTRGSIAGVGLISPPPH
                     HDIYSIEDLKELIFDLKNANRDARINVKLVSEAGVGTIAAGVAKANADTILISGYDGG
                     TGAAPRTSIRNTGLPWELGLAEAHQTLVLNKLRDRVRVETDGKLLTGRDVAIACMLGA
                     EEFGFATTPLVAIGCIMMRVCHLNTCPVGIATQDETLRKNFQGKPEYVVNFMMFVAEE
                     LREIMAKLGFRTINEMVGRSDKLKMKENVKHWKAKTVDLSSILYQPYAAPNVGRYNMT
                     EQDHELEKTLDMRRLLRIVKPAIDAKKRIVAKSAIKNIDRVVGTIIGSEISKKYGAEG
                     LPEDTIQLTFVGSAGQSFGAFIPKGMTLTLEGDSNDYIGKGLSGGKIIVRVPNEATYA
                     PEENIITGNVAFYGATSGKAYINGIAGERFCVRNSGVDAVVEGIGDHGCEYMTGGTVL
                     IIGKTGRNFAAGMSGGVAHILDFDENNLNMEMVSATTDLNDTEHDTIKRML"
     assembly_gap    2897..2921
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2922..4358)
                     /locus_tag="PLT73_00975"
     CDS             complement(<2922..4358)
                     /locus_tag="PLT73_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRM18985.1"
                     /translation="MNSYTPPKKQGMYDPAYEHDACGMGFVVDINGKGSYKLVSDALD
                     LLENMEHRGASGADAKTGDGAGIMVQIPHHFFSRECDVLGIHLPKKGDYGVGMLFAHK
                     YEDLRHEQMRMVEEIITSEGQKVLGWREVPIDSSEIGATAKAAIPRFIQVFIGKGEPQ
                     MTEMDFERKLFVIRKLSEKQIVPMSEKRGGSFYFASLSSKTIVYKGMLTAEQLRSFYL
                     DLTDLDFTSALAMVHSRFSTNTFPSWERAHPNRYLIHNGEINTIRGNINWMRARQGSM
                     SQDLLDASEKIYPIVDETGSDSSQFDNNLEFLYLNGRSLPEAVMMMVPEPWEKNKTMS
                     KSKKDFYKFNNFLMEPWDGPAAMGFTDGEIVGAVLDRNGLRPSRYYVTKDDRVILSSE
                     VGVVDVRPEDVAYKGRLEPGKMLLIDTKQKRIISDEEIKETVAAKQPYGEWAEKHISK
                     MKDLAPAPLNEIVEPEELLHQQKAFGYTF"
     gene            complement(4672..5529)
                     /gene="speB"
                     /locus_tag="PLT73_00980"
     CDS             complement(4672..5529)
                     /gene="speB"
                     /locus_tag="PLT73_00980"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRM18986.1"
                     /translation="MRNNMNIQTFIGCESSFEEASTVLFGIPFDGTTSFRPGTRFAMQ
                     AIRQDSFGLESYSPYLDRDLEEEAIHDGGDLDLPFGNTERVLSDIATYTKEVLDNGKK
                     FLMVGGEHLVSLPTIEAAYEKYPDLHVIHIDAHTDLREDYMGEPLSHASVIRRCFDFL
                     GDGRIFQFGIRSGLKEEFEWAKEHTYMEKFTIDTLPHIVQQLKDVPVYVTIDLDVLDP
                     GTFPGTGTPEPGGITYKELLAGIAALQDLNQIVAADVVELSPQYDPSGASTAVACKTV
                     REMLLTLTK"
     gene            complement(5578..7119)
                     /locus_tag="PLT73_00985"
     CDS             complement(5578..7119)
                     /locus_tag="PLT73_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM18987.1"
                     /translation="MAEKVLFNSDTYLDGHSDKITYQISNVKAKNITHIKMPLFEALV
                     KYAKQDVTPFDVPGHKMGAQMTPFKMAVGDMTMQMDVNSMKELDLLSHPQFVIKEAQE
                     LAAKAYNADQAFFLVNGTTVGIQAMIMSVVGPNDSILVPRNCHKSVMSGLILSGAKPI
                     FIQPEMDLAFGISHGISVERIIKGLDDNPKAKALLVTYPTYFGSLNDLQTICDIAHER
                     GVVVIVDSAHGAHLPFMPGKMKDPMKAGADAVTYSMHKTGGSLTQSSLMVMQGDRISA
                     TKLQKVLNMLQSTSANYLLMSSIDAARSELAMYGKDRYKKLRPIVSEAIEAIEAFGDY
                     EVLKSDYIKDKFDQTYDWTKLVIRVNDIGLTGFEVYTILKQEYGIQMELAEGYVVMAV
                     ISTADTRESIGRLVDALMDIRRRYKKASQHFDAHVMTCSINELACSPRDAFYADNEVV
                     GIDEAVGRISADSIMIYPPGIPLIIPGEVISEAVVQHYHYYNETLGNVLTEEKMPHQI
                     KVLKD"
     gene            complement(7914..9188)
                     /locus_tag="PLT73_00990"
     CDS             complement(7914..9188)
                     /locus_tag="PLT73_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM18988.1"
                     /translation="MNRITQLSIFENNQLGDLEKLNDVLNALPDEKLIALLEEERGKG
                     RNDYPVFTMWRAFLASFVFQHPTVAALIRELKRNSQLREVCGFQTRIIRTKKGSYRYQ
                     LAPTEAAFSRFVKKLLQHQGLLDVMFHELVDEMYVSLDGFGEILALDGKIIPSYAKGP
                     NQNKQEDGRRDTEANFTIKQYTTTTPSGEKVTNKKKWFGYRLHLIIDATYELPIGYEL
                     TKASEGEPTVAKRLIKALPDAQLKSCNYLLADRGYDGGPLLDEIESRDIIPIIDIKNQ
                     WKKEGPTKQYRDTDLTYTYNGKVFYVDERGQEVALVYKGYDSATDSLRYGFKPQHQDS
                     RIFRIKREDNRRVFNKVARNSHKFKRLYKKRTAIERVNGRLDRDFLFEQHTIRGEKKM
                     NLFVTMAFLVMLAFAKRNIQKNELGHLNAWVA"
     gene            complement(9235..>9414)
                     /locus_tag="PLT73_00995"
     CDS             complement(9235..>9414)
                     /locus_tag="PLT73_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18989.1"
                     /translation="ESITLFWSPWGSKDSGCSKIKIAKVKACCYSTFTLAIYCFMDFA
                     TNVKKKKKKRPILLK"
BASE COUNT         2312 a         2318 c         2052 g         2707 t
ORIGIN      
        1 gcagcatgcg cttgattgtg tcatgctctg tatcattcaa atctgtcgtt gcgctgacca
       61 tttccatatt caggttgttt tcatcaaaat caaggatatg agctaccccg ccggacattc
      121 ccgcagcgaa gttgcgtccg gttttaccga tgatcagaac cgttccgccg gtcatgtatt
      181 cacacccgtg gtcgccgatc ccttcaacga ctgcatccac tccgctgttc cggacacaga
      241 accgttctcc agcaatgccg ttgatgtatg ctttaccgct ggttgcgcca tagaaagcaa
      301 cgttaccagt gatgatgttc tcttcaggtg cgtaggtagc ctcattcggt acgcgtacga
      361 tgattttacc accggataag cctttaccga tatagtcgtt cgaatcgcct tccagcgtca
      421 atgtcatccc tttcggaatg aatgcgccga aactttgtcc tgccgatccc acgaatgtca
      481 attggatcgt atcttccggt aagccttcag caccgtattt tttcgatatt tcgctgccga
      541 tgatggttcc gacaacacgg tcgatgtttt tgatggctga tttcgcaacg atgcgtttct
      601 tagcatcaat tgccggtttc acgattctca ataggcggcg catatccagt gttttctcca
      661 gctcatggtc ttgctccgtc atgttgtatc tgccgacatt cggtgctgca tagggttggt
      721 acaatatgct ggacagatcg actgttttcg ctttccaatg cttgacattt tctttcatct
      781 tcagcttatc gctgcgtcca accatttcat tgatcgtacg gaagcctaat ttcgccatga
      841 tttcacgcaa ttcttcggca acgaacatca tgaagttcac gacgtattcc ggtttgcctt
      901 ggaagttttt gcgcaatgtt tcatcctgcg ttgcgatacc cactggacat gtattcaggt
      961 ggcagacgcg catcatgatg cagccgatag ccactagcgg tgtcgtcgca aagccgaatt
     1021 cttcggcacc caacatacac gcgatagcaa catcgcgacc agtcaataat ttaccatccg
     1081 tttcgacgcg gacacggtca cgcaatttgt tcaacaccaa tgtttggtgc gcttcagcca
     1141 agcccagttc ccacggcaag ccggtattac ggatgctggt tcttggtgct gcacctgtac
     1201 cgccatcata cccgctgatc aggatcgtat cggcatttgc tttcgcaaca cctgcagcga
     1261 tcgtaccgac gccggcttca gaaaccaatt tcacgttgat gcgcgcatca cgattggcat
     1321 ttttcaagtc aaaaatcaat tctttcaagt cttcgataga gtagatatca tgatgcggag
     1381 gcggtgaaat caagcctaca cctgcgatgg aaccacgtgt tttggcgatt tcaggatata
     1441 ctttcggtcc tggcaattgt ccgccttcac ccggttttgc accctgggcc atcttgattt
     1501 ggatttcctt cgcattcacc aagtagtggc tcgtaacgcc gaaacgtccg gaagcaactt
     1561 gcttgatggc actgttgcgg gaatctccgt tttcatccaa ttcgaaacgg cgcggatctt
     1621 ctccaccctc accagaattg ctcatcccgc cgagacggtt catagcgatc gctaaggttt
     1681 ggtgcgcttc gatactgatc gaaccgtaag acatcgctcc ggtcttgaag cgtttcacga
     1741 tgctttcagc cgattcgact tcttcgatcg gaatcgcatc ttgagcattc ggcttgaatt
     1801 gcatcagttg acgcagcgtg taggcgttcg tgtccgcatt gatttcgcgg gagaactctt
     1861 tgtattgctc atagtctcct tgacgagtcg cctgctgcag acggtagatt gtgctcggat
     1921 tgtacaagtg ttcttcaccc atggccgagt attggaaatg accgcccact cccaacgttt
     1981 cgctgtaagg cgctgtagct ttataagcac tgtcatgacg cattctgttt tcagtggcga
     2041 tttcctgcag tccgatacct tcaatacggg tcggcgtgcc tgtgaaatat ttagcgacaa
     2101 cttcactgtt gactcccagt gcttcaaaaa tttgtgcacc gtgataactg cgcattgttg
     2161 aaatacccat tttcgtcagt actttggaca taccatgtac agccgcgtgg atgtaattcg
     2221 cttccgctgc ttcagcatcc agtccgtcca gcagatcttc ttcagcaaga tcgcggatgg
     2281 tcgcgtaagt caagtacgga ttgaccgcat tacagccgta tccgaccaat gtacagaagt
     2341 gatgcacttc tcttggttct ccggactcca gcacaaggcc gacgttcgtg cgtgtctctt
     2401 cgcggaccaa atgatgatgc aggccggaaa tagccaataa agccggaatg gcagcccatt
     2461 cagcgttcac accacggtcg gaaaggacca gaatgttggc gccggaagca atggctttgt
     2521 ccgctgcact gaacagacgt tccaaagctt tttccatccc cttcgcatcg tcttcgacac
     2581 gatataaaag agaaagcgtg acagttttga atttatcgtc ctgcagcgct ttgattttcg
     2641 ccagttcgga gtccgtcaaa atcgggctat ccaagtagat ggcatgcgtg tctgcattat
     2701 ccggatccaa taaattcccg caatttccga caagcatggt gtcggaagta acgatttcct
     2761 cacggatacc gtcaatcgga ggattcgtaa cttgcgcaaa taactgtttg aagtacagat
     2821 acagcatctg cggtctgtcc gataagactg ccaacggaga atcgatcccc atggcgccga
     2881 ctgaatcagt tcccgtnnnn nnnnnnnnnn nnnnnnnnnn ngaatgtata accaaaagct
     2941 ttctgctggt gcagcaattc ttcaggctca acaatttcgt tcaatggagc aggcgcaagg
     3001 tccttcattt tggaaatgtg cttttcagcc cattctccat aaggttgttt agccgcgacc
     3061 gtttctttga tttcctcatc cgagatgatg cgtttctgct tcgtatcgat caacagcatc
     3121 ttacctggtt ccagacgacc cttataggcg acatcttccg gacgcacatc tacgacaccc
     3181 acttcggatg aaaggatgac gcgatcatct ttcgtcacat agtaacggga cggacgcaaa
     3241 ccgtttctgt ccaataccgc tccgacgatt tccccatccg tgaagcccat tgctgcaggt
     3301 ccatcccatg gttccatcaa aaagttgttg aatttataaa aatctttttt ggatttagac
     3361 attgttttgt tcttttccca aggctcagga accatcatca tgacggcttc cggcaatgag
     3421 cggccgttca gatacaagaa ttccagattg ttgtcgaatt gggaagaatc actccctgtt
     3481 tcatccacga tcgggtagat tttttcactg gcatccagca aatcctggga catgctgcct
     3541 tgacgggcac gcatccagtt tatgttgccg cggattgtgt tgatttcgcc attatggatc
     3601 aagtaccggt tgggatgggc tctttcccag ctcggaaagg tattcgtact gaaacgggaa
     3661 tggaccatcg ccaaagcgga tgtgaagtcc agatccgtaa gatcaagata gaaactgcgt
     3721 aattgttctg cagtcaacat ccctttgtag acgatcgttt tcgaagataa actggcaaaa
     3781 tagaacgatc cgcctctttt ttcgctcatc ggaacgattt gtttttcgga tagtttgcgg
     3841 atgacgaata atttgcgttc gaaatccatt tccgtcattt gaggttctcc tttaccgatg
     3901 aagacctgga taaacctcgg aatggcagct ttagcagtcg caccgatttc cgaactgtcg
     3961 atcggaacct cacgccagcc cagcactttt tggccttcgg aagtaatgat ctcttccacc
     4021 attctcattt gttcgtgacg caaatcttca tacttatggg caaagagcat ccctacacca
     4081 tagtcgcctt ttttaggcaa atggattccg aggacgtcgc actccctgct gaaaaaatga
     4141 tgtggaatct gcaccatgat acctgctccg tcacctgttt tggcatcagc gccggaagca
     4201 cctctatgct ccatgttttc caataaatcc aacgcgtcag agactaattt ataagaaccc
     4261 ttaccattga tgtcaacgac aaatcccata ccgcatgcat catgctcgta ggcgggatca
     4321 tacattcctt gcttttttgg aggagtgtaa ctattcattt cggcgtccct actttctatt
     4381 ttgttattct gatattataa tcaaactctc atttaattac aatccatttt cattcatttt
     4441 ttcacaatta gttgcatatt ttttttgata gtaacaatat taacttctag aaaaacattc
     4501 gctataacag gatttcacta gcataaggat ataaaaagtt tgtgatactt ttccttacat
     4561 gcctacgtta gattcgggaa ttttcattac attttaatgt aaatattttc attcggaaat
     4621 cagggcaaaa aaaagacctt ggttatgtcc aaggtccaat aatcgattct tttatttagt
     4681 aagcgtcaaa agcatttctc tgacagtctt gcaggccaca gcagtagaag cgcctgaagg
     4741 atcgtattgc ggtgaaagtt cgacgacgtc ggctgcaacg atctgattca agtcctgcaa
     4801 agcagcgata cccgctaaca attccttgta ggtgatgccg cctggctcag gtgtgccggt
     4861 tcccgggaaa gtaccaggat ccaaaacgtc cagatcgatt gtcacataaa cgggaacatc
     4921 cttcaattgc tgcacgatat gcggcaacgt atcgatggtg aatttctcca tataagtatg
     4981 ctctttcgcc cattcgaact cttccttcaa accggagcgg ataccgaatt ggaaaatgcg
     5041 gccgtctccc aggaaatcga agcaacggcg gatgaccgaa gcatgcgaca acggttcgcc
     5101 catgtagtct tcacgcagat ctgtatgggc atcgatatgg atgacatgca gatccggata
     5161 tttttcatag gcagcctcga tggtgggcag gctgacgagg tgctcgccgc cgaccatcaa
     5221 aaatttcttg ccattatcca atacttcttt tgtgtaggtt gcgatatcac tcaagacgcg
     5281 ctctgtgttg ccgaaaggta gatccagatc tccgccatca tggatggcct cttcttcaag
     5341 atcgcgatcc aaataaggag aataactttc caagccaaat gaatcttggc ggatcgcctg
     5401 catcgcaaag cgcgtgcctg gcctgaaact ggtcgttcca tcgaatggaa tcccgaataa
     5461 tacggtggat gcttcttcaa aagaagattc gcagccgata aaagtctgga tgttcatatt
     5521 atttctcact gtctgccgtc ctccaactgt atgctatttg atggttgatt tgattgttta
     5581 atctttcagg actttgatct gatgaggcat tttttcctcg gtcagaacgt tgcccagtgt
     5641 ctcattatag tagtggtagt gttgcacgac cgcttcggag atcacttcac caggaatgat
     5701 caacggaatt cctggcgggt agatcatgat cgagtcggca ctgatgcggc cgactgcttc
     5761 gtcgatgccc accacttcat tgtcggcata aaaggcatca cgcggactgc atgccaattc
     5821 gttgatactg cacgtcatca cgtgggcatc gaaatgttga ctggcttttt tatagcgcct
     5881 gcggatgtcc atcaaggcat caaccagacg tccgattgat tcccgcgtat cggccgtgct
     5941 gatgacagcc atcacgacat agccttcagc caactccatt tggatgccgt actcttgctt
     6001 caagatcgtg tagacttcaa agcccgtcag gccgatgtca ttgacacgga tcaccaattt
     6061 ggtccagtca taagtctggt cgaatttgtc tttgatgtag tcggatttca atacttcata
     6121 gtcgccgaat gcttcgattg cttcaatcgc ttcactgacg atcggacgca gttttttgta
     6181 gcgatctttt ccgtacatcg ccaattcgct gcgtgcggca tcgatggaac tcatcaacaa
     6241 gtagttcgcg ctggtcgatt gcagcatgtt cagcactttt tgtaattttg ttgcggaaat
     6301 gcggtcccct tgcatcacca ttaaggaact ctgcgttaag gatccgccgg ttttatgcat
     6361 ggaatatgtc accgcatcgg ctccggcttt cattggatcc ttcatttttc cgggcatgaa
     6421 cggtaaatgc gccccgtgcg cactgtcgac gatgacaacg acaccgcgtt catgcgcgat
     6481 atcacagatc gtctgcaaat cgttcaacga tccgaaataa gtcggataag tgactaaaag
     6541 agctttcgct ttgggattat catccaaacc tttgatgatg cgttccacgg agatgccgtg
     6601 agaaataccg aaagccaaat ccatttcagg ctgaatgaag atcggctttg caccgctcag
     6661 gatcaaccca ctcatcactg acttatggca gttgcgcgga accaagatgg aatcgttcgg
     6721 cccaacaacg ctcatgatca tcgcttggat gcctacagtc gtaccgttca ccaagaaaaa
     6781 ggcctgatcg gcattgtatg cttttgcagc cagttcctgt gcttctttta tgacgaattg
     6841 cggatgactc aacaagtcca attctttcat ggagttgaca tccatctgca tcgtcatatc
     6901 accgaccgcc atcttgaatg gcgtcatctg cgcacccatt ttatgtccag ggacatcgaa
     6961 aggtgtaacg tcctgtttcg catatttaac aagcgcttcg aaaagcggca ttttgatatg
     7021 tgtaatattt tttgctttaa cgttagaaat ttggtaagta attttgtcgg aatgaccgtc
     7081 caaataagtg tctgaattga ataaaacctt ttcagccaaa tagatttccc cctttgatat
     7141 aaaaaatggg cataaaaaaa gctctccaac caaaggaaag cttcgatatc tatcagctgc
     7201 ttttcctctt attgctcata ggacttggat aatcctacgc tcaggttggg cacccaatat
     7261 caatccttga aaataaaatt agattgattg gtttgccacc gtatcttcga cccctcgtct
     7321 caacggctat taataagaga tattcaattt atgcaagcta aattctatac aaatacaacg
     7381 tatatgtcaa tgattttgga aagaaaacta aaattttttc agttggcatt tccgaacaat
     7441 gatttgacag ttgttcacga tcatccacaa aaaagctgaa attcttcaga tccagagttt
     7501 agtttattcg gataggttga aattaagatt acatcccctt ctccggtgaa cgggtgctta
     7561 gtcggagttc aggatgaatt tgccgtcacg ctccggcgag ggccagcttg ccggagttgc
     7621 gttaacattt cgcgctgtcc tccgctgaac atccggttca ccggaggaca ggcttgaata
     7681 ttgggtcatg ccttcgcccg atagtcaagc gcatcaaaaa aagaatcaat tacgctgttc
     7741 gctttttttg tagaattgaa agagaaaaaa caaagccgat ggctttttca gtcgcaaata
     7801 tgcagaatat gggcctctta ataccgatca gtatgtgtgg gcataagcag gattctgttt
     7861 ttttcatacg aaggcatgac aaagaaacgt acggatattc tattttttag aggttacgct
     7921 acccaagcat tcaaatgacc caactcattt ttttggatat tccgtttagc gaatgccaac
     7981 atgaccagaa aagccatggt tacgaaaagg ttcattttct tttcaccccg tatagtatgt
     8041 tgctcaaaca agaaatcacg atcaagacgc ccattcaccc gttcaatcgc agtccgcttc
     8101 ttgtacagcc gcttgaattt gtggctatta cgcgctactt tattgaaaac acgccgatta
     8161 tcctcccgtt tgattctgaa aatacgggaa tcctggtgtt gcggtttgaa tccgtaacga
     8221 agggaatcag tcgcactatc atagcctttg taaaccaacg ccacttcctg tcctctttca
     8281 tcgacataga aaactttccc gttataagta taggtcaaat cggtatcacg gtattgttta
     8341 gtaggtccct cttttttcca ctggttctta atatcgataa tcggaataat gtctctcgat
     8401 tcaatttcat ccagcagggg gcctccgtca taccctctgt ctgcaagaag atagttgcag
     8461 cttttcaatt gagcatccgg caatgccttt attagtcttt tcgcgaccgt aggctcgcct
     8521 tccgaagcct ttgtaagttc atagccaatc ggcagttcat aagtggcatc gatgatcaaa
     8581 tgcaatcgat agccgaacca cttcttcttg ttcgtcactt tctctcctga aggtgtagta
     8641 gtggtgtatt gtttgatggt aaagttcgcc tcagtgtccc ttctgccgtc ttcttgttta
     8701 ttctgattgg gccctttcgc gtaggaggga atgattttcc catctagagc gaggatttcg
     8761 ccaaacccat ctagggagac gtacatctcg tccactagct cgtggaacat aacatccagc
     8821 aaaccttggt gctgcaacag tttctttacg aaacgggaga aagcggcttc cgtgggagcc
     8881 aactgataac gataagaccc ttttttcgta cggatgatcc gagtttggaa tccgcatacc
     8941 tcccggagtt ggctgttgcg cttcagttcc cggatcaaag ctgcaactgt ggggtgttgg
     9001 aacacgaaac tggcaaggaa agcccgccac atggtgaaca caggataatc attccgcccc
     9061 ttcccacgtt cttcttccaa aagggcgatg agtttctcgt ccggcaatgc attcagcacg
     9121 tcatttaatt tctctaaatc cccgagctgg ttattttcaa aaatggataa ttgtgttatt
     9181 ctgttcatag aaaaagcgac ctccttaata ttttgtagtg actatatttt actgctattt
     9241 cagcagtatg gggcgctttt tctttttttt ttttacatta gtcgcaaaat ccataaaaca
     9301 atagatcgcc aaggtgaaag tgctgtaaca gcaggctttc accttggcga tttttatttt
     9361 gctacatccg ctatccttag agccccaagg gctccagaac agcgtaattg attc
//
LOCUS       DAOMUJ010000017         9184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1904_length_9184_cov_3.274072, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000017 DAOMUJ010000000
VERSION     DAOMUJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_9184_cov_3.274072"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1276
                     /locus_tag="PLT73_01000"
     CDS             179..1276
                     /locus_tag="PLT73_01000"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HRM18990.1"
                     /translation="MAYRVFRSPSRYIQGVGAIDILGKETAVYGKRAFLLTDDFVWGL
                     LQEKVERALEGFPYHYEAFTGEASIDEMVRLADAVADVTADVVIGLGGGKIIDVAKGI
                     AAACDLPVVIVPTTVSTDAPTSAISVLYTEDGLFDHYRFYAKNPDLVLVDTQIVIQAP
                     IRFFASGMADALATCAEALATKASGENALAGGLPTIAGTAIAEACEETLFREGLSAFA
                     AVQKGLVTPAVEAIVEANTLLSGLGFENGGLAAAHAIHNGFTAIKGEVHRLTHGEIVG
                     FCLLVQLILEERPQEEFKRYSRFLAAIGMPTTLAEMHLTGATREELLEIGKLAVAPSN
                     TMKNLNKNITAEQVVAAILAVDTFIGKEEKE"
     gene            1273..2385
                     /gene="ald"
                     /locus_tag="PLT73_01005"
     CDS             1273..2385
                     /gene="ald"
                     /locus_tag="PLT73_01005"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRM18991.1"
                     /translation="MIIGVPKEIKNNENRVGVVPAGVQMLVQNGHQVMVESGCGVGSG
                     FEDGQYEKAGAKVLADAGQVWAAEMVIKVKEPLESEYKYFREDLILFTYLHLAAAPKL
                     TEAMLQGGLTAIGYETMVGKKGDLPLLTPMSVIAGRLSVQIGAQFLEKPYGGKGILLS
                     GVPGVANGKVVIIGGGVAGRNALQIALGLGAHCTILDVRPEILTEIENLYGNAVITLL
                     SNEWNIAHAIKDADVVIGAVLIPGRKAPTLVTEEMVKSMQPGSVIVDIAVDQGGIFET
                     EDRTTTHDNPVYEKYGVLHYAVPNMPGAVARTATIALTNVTLPYALEMADQGAVQAAR
                     NNPTILTGFNVHNGRLTNKDVAESVGAVYTPIETLL"
     gene            2517..3590
                     /locus_tag="PLT73_01010"
     CDS             2517..3590
                     /locus_tag="PLT73_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM18992.1"
                     /translation="MKTKYQVIADEIRSKILSNEFVVGKVIPPELQLQKDYGVSRHTV
                     REAIALLVNEGFLRKEKGSGTYVDDTYQRRGDGVSSSKTIGVITTYLSDYIFPSIIRG
                     IEQKLRENGYSLLLASTNNDVDQERECLEQMLSQGVSGLIVEPTKSNQYNPNLAYYLS
                     FKERGIPVVMINANYEELSVPFICVNDTKAGYLATKHLFEQGHQHLALITKIDDLQGK
                     YRMKGFIKAHEEMRTNFDPKNVYTYTTETKAQVFQTIREKLASDQEVTGIVCYNDEVA
                     QGLIQALADMGKRVPEDYSVVGNDDSFLSTAGSVKLTTLSHPKEELGAAAAEWIMLAV
                     QQEVTGNKIFEPELIIRDSVKRI"
     gene            3756..5366
                     /locus_tag="PLT73_01015"
     CDS             3756..5366
                     /locus_tag="PLT73_01015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM18993.1"
                     /translation="MTEKLQAIVTDIESRQTSIGIEFGSTRIKAVLIDSRFAPIASGS
                     YEWENQLVEGIWTYSLDQIWKGLQTSYAELTREVKEKYGVTLTAVGSIGFSAMMHGYM
                     AFNQENELLVPFRTWRNATTADAEKELTALFKYNIPQRWSIAHLYQAVLNKEEHTNEV
                     AFFTTLAGYIHWQLTGKKVLGIGDASGMFPIDVEKKNYDERMIGQFDALVAEKGATWK
                     LADLLPEVLLAGDAAGNLTEVGAKLLDPTGTLEAGIPLCAPEGDAGTGMVATNSVAER
                     TGNVSAGTSAFAMIVLEQDLNEVHPEIDLVTTPAGSLVAMVHTNNCSSDINAWVKLFR
                     EFSEAAGFTIDTDKLFATLFNKALEGDADCGGLLSYGYYSGENITHLAEGRPLFVRTP
                     ESNFNLANFMRVHLFTAFGAMKIGMNILKQEHVAIDKIVGHGGIFKTPEVGQKVLAAV
                     MDAPVTVMETAGEGGAWGIALLAAYMDRKETSESLDAFLDKKVFGADEGITIAPDVRD
                     VEGFGTFIERYQEGLAIEQAAVEHLSLR"
     gene            5383..6081
                     /locus_tag="PLT73_01020"
     CDS             5383..6081
                     /locus_tag="PLT73_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM18994.1"
                     /translation="MLEQLKEEVFQANLELPERGLIKYTWGNVSAVDREKGLFVIKPS
                     GVGYDDMKASDMVVCDFDGNVIEGDLNPSSDMPTHAVLYKEFPEIGAVVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGTVPCARFLTQAEIDRGYEEETGNAIVETFRERGIKPM
                     EVPGVLLHGHGPFTWAKNAKGAVLNAVVLDEVCKTNLFARQLNAFAEELPQRILDKHY
                     LRKHGENAYYGQKK"
     gene            6095..7519
                     /gene="araA"
                     /locus_tag="PLT73_01025"
     CDS             6095..7519
                     /gene="araA"
                     /locus_tag="PLT73_01025"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRM18995.1"
                     /translation="MLEVGKKEFWFVVGSQHLYGEEALQTVKNNAAVIVESLNNSGKL
                     PYPLVLQELAVTPDTITKIMKEVNYRDEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQFNESIPWATIDMDFMNLNQAAHGDREYGFINARLKKNNKVVVGYWERENVQTQI
                     AEWMDVAVAYNESFSIKVARFGDNMRNVAVTEGDKVEAQIQFGWTVDYYGIRDLVDYV
                     DAVADEEVDALFNEYKDLYDFDYGDYEQGKWEAHVKVQARQEIGIRRFLEAGGYTAFT
                     SNFEDLHGLQQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLMKIMSHNIDTGFMEDYT
                     YEMAEGREAILQSHMLEVDPGLAVNKPKILIAPLSMGNREEPARLVFDGKAGDGVVVS
                     MADFGTHYKLLVNEVVAFEPTEAAPNLPVARVLWEVKPNFHDGIRAWIEAGGGHHTVV
                     SLSLSTDQILAWAKLVGLEVALIK"
     gene            7629..>9184
                     /locus_tag="PLT73_01030"
     CDS             7629..>9184
                     /locus_tag="PLT73_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRM18996.1"
                     /translation="MTAFDSKAYLDKVDAFWRAANFISVGQLYLKDNPLLQRPIEATD
                     VKLHPIGHWGTISGQNFIYAHLNRVINKYDLNMFYVEGPGHGGQVMVSNAYLDGSYTE
                     IYPDITEDLEGLTKLYKQFSFPGGVASHAAPETPGSIHEGGELGYSLSHATGAIFDNP
                     EVIAATVVGDGEAETGPLAAGWFSNVFINPVTDGAVLPILYLNGGKISNPTILDRKSN
                     EELTQYFNGMGWEPLFVEGTDPEAVHPIMAQVLDTAVEKIKAIQTEARKGSAAEAKMP
                     VWPVIIFRTPKGWTGPQTWDGEPIEGGFRAHQVPIPVDAHHMQHIDALVDWMQSYRPE
                     ELFTAEGQLVAELKEMAPKGDRRMAMNPVTNGGIDPKPLNIPDWKQYAVDTTVPGAVI
                     AQDMIEFGNFARDLVVDNPDNFRIFGPDETKSNRLNKVFEVTNRVWMEAIDPAYDEWL
                     SPSGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYEAFLRVVDSMITQHFKWLRKAKE
                     QTWRKHYPSL"
BASE COUNT         2427 a         2186 c         2567 g         2004 t
ORIGIN      
        1 gtacggtgat gcacttctga gttgaaaaaa atttataaat tcctatatgt taaaatcaga
       61 atccttcgga agcccaaaag ctccgaagga tttttttttt gaccagaaaa aaacggggcg
      121 taattgtggt aaaatcatta tatgataatt aaatcacaat tggaaaaggg gcgcgcatat
      181 ggcttatcgt gtttttcggt cgcctagccg gtatattcaa ggagtaggcg ctatagacat
      241 attagggaag gaaacggctg tctatgggaa aagggcattc ctgcttacgg acgatttcgt
      301 ttggggcttg ctccaggaaa aagtggaacg ggcgctggag ggttttcctt accattatga
      361 agccttcact ggcgaagcct cgattgatga aatggtccgt ttggctgacg cggtcgccga
      421 tgtcactgcg gatgtggtca tcggtctcgg cggcggcaaa atcatcgatg tcgcgaaagg
      481 catcgcagcg gcttgtgatt tgccggtcgt gattgtcccg accaccgttt ccacggatgc
      541 accgaccagc gccatctctg ttctctatac cgaggacggg ttgttcgatc attaccggtt
      601 ctatgcgaag aaccctgatt tggtgctcgt ggatacgcag atcgttattc aagcgccgat
      661 tcgattcttc gcaagcggta tggcggatgc cttggctacc tgtgcagaag cgctggcaac
      721 gaaagcctcg ggggagaatg ctttggccgg cggtttgcca acgattgccg gcaccgctat
      781 tgcggaagcg tgcgaagaaa ccttattccg tgaaggactc agcgcattcg ctgctgtcca
      841 aaaaggcttg gtgacgccgg ctgtcgaggc gatcgtcgag gcgaacacgc tgctgtcggg
      901 actgggtttt gaaaatggtg gcttggccgc tgcccatgcc atccacaacg gattcacggc
      961 catcaaaggc gaggtccatc gcttgacgca tggggaaata gtcggctttt gcctgttggt
     1021 ccaactcata cttgaagagc gcccgcaaga agaattcaaa aggtatagcc gtttcctggc
     1081 cgcaatcggg atgccgacga ctttggccga gatgcatctg accggagcca cgcgtgaaga
     1141 gctccttgaa atcggaaagc ttgcagtggc gccgagcaac accatgaaaa atctaaacaa
     1201 aaacataacg gctgagcaag tcgtcgccgc cattttggca gtggatacat tcatcggaaa
     1261 ggaagaaaaa gaatgatcat tggagtaccg aaggaaatca aaaataacga aaaccgcgtg
     1321 ggagtagtgc cggcaggtgt gcagatgctg gtacagaacg gccaccaggt aatggtcgaa
     1381 tccggatgcg gtgtcggatc gggattcgaa gacggacaat acgaaaaagc gggagcgaaa
     1441 gtgttggcag acgcaggcca agtctgggcc gcggaaatgg tcatcaaagt gaaggaaccg
     1501 ttggaatcgg agtacaaata cttccgcgaa gacctgattc tgttcacata cctgcacttg
     1561 gcggccgcac cgaaattgac ggaggcgatg ctgcaagggg gcctgaccgc aatcggctac
     1621 gaaacgatgg tcggcaagaa aggggacttg ccgttgctga cgccgatgag cgtcatagcg
     1681 ggccgtcttt ccgtccagat cggtgcgcag ttcctggaaa aaccatacgg cggtaaaggt
     1741 attctgttga gcggcgttcc gggtgttgcg aatgggaaag tcgtcatcat cggtggcggt
     1801 gtggccggac gcaatgctct gcagattgcg ttgggtttag gtgcccattg cacgatcctg
     1861 gacgtcagac cggaaatatt gacggaaatc gaaaatttgt acggaaacgc ggtcattacg
     1921 ttgctgtcga acgagtggaa cattgcccat gccatcaagg atgctgatgt cgtcatcggg
     1981 gctgtcctga tcccaggccg gaaagcgcct acgttagtga ccgaagaaat ggtgaaaagc
     2041 atgcagccgg gttccgtgat cgtcgacatc gccgtggatc aaggcggtat tttcgaaacc
     2101 gaagatcgca cgaccaccca cgacaatcca gtctatgaaa aatacggcgt cctgcattat
     2161 gcggtgccga acatgcctgg tgccgtcgca cgcactgcca cgattgcgtt gacaaatgtg
     2221 accttgcctt atgctttgga gatggctgat cagggagcag tccaagcggc aagaaacaat
     2281 ccgaccatcc tgaccggatt caacgtgcac aacggcaggt tgacgaataa agatgtcgcc
     2341 gaatcggtcg gtgcagtcta caccccaatc gaaactttgt tgtgaaagtt accggaatcc
     2401 ctgctatatc aagcagggat tccgtttttt gcatagtatc catcaatggg cacttgtatt
     2461 tttctgcaca gtttactata ctatttgtac ggacatatat agagggagaa catacgatga
     2521 aaacaaaata ccaggtgatt gccgatgaga tacgctcaaa aatactttcg aacgaattcg
     2581 tggtaggtaa agtcattccg cccgaactgc aattacagaa ggactatgga gtcagccgcc
     2641 ataccgtccg ggaagcgatc gccttgttgg tgaatgaagg ctttttgcgc aaggagaaag
     2701 gttccgggac ttacgtcgac gatacgtatc agagacgtgg tgacggagtg tcttcgagca
     2761 aaacgatcgg cgttatcacg acgtatctgt cggattatat tttcccgtcg atcatccgcg
     2821 gcatcgagca gaagctgcgg gaaaatggct actccttgct gttggcgagc acgaacaacg
     2881 atgtcgatca ggagcgggag tgtctggaac agatgctcag ccaaggcgtc tcggggctga
     2941 tcgtggaacc gacaaaaagt aaccaataca atccaaattt ggcttattat ctatccttca
     3001 aggaacgcgg cataccggtc gtcatgatca atgccaacta tgaggaactg tcggtcccgt
     3061 tcatttgcgt caatgacacg aaagcgggct atctcgctac gaaacatctg ttcgagcaag
     3121 gccatcagca tttggcgctg atcacgaaaa tcgatgacct gcaagggaag taccgcatga
     3181 aaggcttcat caaagcccac gaagagatgc gtacaaattt cgatccgaaa aatgtctaca
     3241 cgtacacaac agagacgaag gcgcaagttt tccaaacgat tcgggagaaa ctggcgagcg
     3301 atcaggaagt caccggaatc gtctgctaca acgatgaagt ggcgcagggc ttgatccaag
     3361 cgcttgctga catgggcaaa cgggttccgg aagattattc agtggtcggc aacgacgatt
     3421 cgttcctgag cacggcaggc agcgtcaagc tgacgacgct ttcccatccg aaagaagaat
     3481 tgggagcggc agctgctgaa tggatcatgc tggccgtaca gcaagaagtg acaggcaaca
     3541 aaatattcga gccggagctg atcatccgcg attcggtcaa acggatctga taactgaaga
     3601 atgaagctgt cccaaaaacc gggatggctt ctttttttgt gttcggacaa atattcaaat
     3661 tactccttta catgtacgga catatagaat ataatgaatc caagaagaac ggacagcatc
     3721 cgcaaaaggg tgtaccgcaa aaaggaggaa atattttgac tgagaagtta caagcgatag
     3781 tcactgatat cgaatcgcgg cagacgagca tcggaatcga attcggctcc acccgcatca
     3841 aggcggtgct gattgactcc cgcttcgctc cgatcgcatc cggcagttac gaatgggaaa
     3901 accaattggt ggaaggcatt tggacgtatt cattggacca gatttggaag ggccttcaga
     3961 caagctatgc agaactgacg cgcgaggtta aagagaaata cggcgtgacc ttgaccgcag
     4021 tcggatcgat cggtttcagc gcgatgatgc acggctacat ggcgttcaat caggaaaatg
     4081 agttattggt gccgttccgg acttggcgca acgccactac ggctgatgcc gaaaaggaat
     4141 tgaccgcact gttcaaatac aatatcccgc aacgctggag cattgctcac ctgtatcaag
     4201 cggttttgaa taaggaagaa catacaaatg aagtcgcttt ctttacgact ttggcaggct
     4261 acatccattg gcaactgaca ggcaagaaag ttttgggaat cggcgacgca tccgggatgt
     4321 tcccgatcga tgtcgagaag aaaaattacg acgaacggat gattggtcag ttcgatgcat
     4381 tggtcgcaga aaaaggcgcg acttggaaat tggcggatct gctgccggaa gtactgttgg
     4441 caggggacgc agccgggaat ctgacggaag tgggggccaa actgcttgat ccgacaggca
     4501 cactcgaagc gggcatcccg ttatgtgcgc cggaagggga cgccgggacc ggcatggtcg
     4561 ccacgaacag cgtggcggag cgcaccggaa atgtatccgc agggacttcg gcatttgcga
     4621 tgatcgtatt ggagcaggac ttgaatgaag tgcatccgga aatcgatctc gtgacgacac
     4681 cggcaggaag cctggtcgcg atggtgcata ccaataactg ttcatccgat atcaacgcct
     4741 gggtcaagct ttttcgggaa ttcagcgaag ctgccggttt tacaattgat acggacaaat
     4801 tattcgctac gttattcaac aaagcccttg aaggcgatgc cgattgcggc ggattgctct
     4861 cgtacggtta ctactcgggc gagaacatca cccatttggc ggaaggtcgg ccgttgtttg
     4921 tccgcacacc ggaaagcaac ttcaacttgg ccaacttcat gcgcgtgcac ctcttcacag
     4981 ctttcggggc aatgaagatc ggcatgaaca tcctgaagca ggaacatgtc gcaatcgaca
     5041 agatcgtcgg ccatggcggc atcttcaaaa cccctgaggt cggtcagaaa gtactggctg
     5101 ccgtcatgga cgcaccggtc accgtcatgg agacagcggg agaaggcggg gcatggggca
     5161 tcgcattgtt ggctgcctac atggaccgca aggaaacgtc agagagtttg gatgcatttt
     5221 tggacaagaa agtattcggt gccgatgaag gcatcacgat cgctcctgat gtgcgcgacg
     5281 ttgaaggttt cggaaccttc atcgaacgtt accaagaagg gctggcgatc gaacaggctg
     5341 cagtcgagca tttgagtttg agatagaagg gaaggaaaca tcttgctgga acaattaaaa
     5401 gaagaagttt ttcaagccaa tctggaattg ccggaacggg gattgatcaa atacacctgg
     5461 ggaaacgtca gtgcggttga ccgcgaaaaa ggcttgttcg tcatcaagcc cagcggtgtc
     5521 ggctatgacg acatgaaagc gagcgatatg gttgtctgcg actttgacgg caacgtcatc
     5581 gaaggggatc tgaacccgtc atccgacatg ccgacgcatg ccgtcctcta caaagaattc
     5641 ccggaaatcg gcgcggttgt gcacacacat tcgacttggg ccacgatttg ggcgcaagct
     5701 ggattggatg tgccggccat gggtacgaca catgccgata ccttctacgg cacggttccc
     5761 tgcgcgcgtt tcctgacgca agcggaaatc gaccggggtt acgaggaaga aacgggaaac
     5821 gccattgtgg aaacgttccg cgaacgcggc atcaaaccga tggaagttcc cggcgtattg
     5881 ctgcatgggc acgggccctt cacttgggcc aagaatgcga agggtgctgt actgaatgcg
     5941 gtcgtattgg acgaggtctg caaaaccaat ctgttcgccc gtcaattgaa tgccttcgcg
     6001 gaagaactgc cgcaacgcat tctggataaa cattatttgc gaaaacacgg agaaaacgcc
     6061 tattacgggc agaaaaaata gaggagctgg agatatgtta gaagtaggaa aaaaagaatt
     6121 ttggttcgta gtcggttcac aacatttata tggtgaagaa gctttacaaa cagttaaaaa
     6181 caatgcggct gttatcgtgg aaagcctgaa caacagcggc aaactgcctt atccgcttgt
     6241 gttgcaggaa ttggcggtaa cgcccgatac gatcacgaag atcatgaaag aagtgaatta
     6301 ccgtgatgaa gtggcaggtg tgatcacttg gatgcatacc ttctcgccag ccaaaatgtg
     6361 gatccgcgga acgaaactgt tgcaaaaacc gttattgcat ctggcaactc aattcaacga
     6421 aagcattcca tgggcaacaa ttgatatgga tttcatgaac ttgaaccaag cggcacatgg
     6481 cgaccgcgag tacggcttca tcaatgcgcg cctgaaaaag aacaacaaag tcgttgtcgg
     6541 ctattgggaa cgtgaaaatg tccaaaccca aatcgcggaa tggatggacg tggcggtagc
     6601 ctacaatgaa agcttctcca tcaaagtggc gcgtttcggc gataacatgc gtaacgttgc
     6661 cgtgaccgaa ggcgacaaag tggaagcaca aatccaattc ggctggacag tcgattacta
     6721 cggcatccgt gatctagtgg actatgtcga tgcggtcgca gacgaagaag tcgatgcttt
     6781 gttcaatgaa tacaaagatt tgtatgattt cgactacggc gattatgagc aaggcaaatg
     6841 ggaagcgcac gtcaaagtgc aggcgcgtca agaaatcggc atccgtcgtt tcttggaagc
     6901 gggcggctat actgccttta cctcaaactt tgaagatctg catggcctgc aacaattacc
     6961 aggtctcgca gtacaacgtt tgatggcaga aggctacgga ttcgcaggcg aaggcgactg
     7021 gaaaacagca gcaatcgacc gcttgatgaa gatcatgtcc cacaacattg acaccggctt
     7081 catggaagac tacacgtatg aaatggctga aggcagagaa gcgattctgc aatcgcatat
     7141 gttggaagtc gatccaggac tggcagtcaa caaaccgaaa attctgatcg caccattatc
     7201 gatggggaac cgtgaagagc ctgcgcgcct tgtattcgac ggcaaagcag gggacggcgt
     7261 tgtcgtttcg atggctgact tcggaacgca ctacaaattg ttggtgaatg aagttgttgc
     7321 ttttgaaccg actgaggcag ctccgaacct tcctgtcgca cgcgtccttt gggaagtgaa
     7381 accgaatttc catgacggca tccgcgcttg gatcgaagct ggcggcggtc accacactgt
     7441 tgtttcattg agtttgagca cagatcagat cctggcttgg gcaaaacttg tcggtcttga
     7501 agtagcctta atcaaatagt ttttttcgat cggtttgaca gaaaatcgtc aaaccgattt
     7561 tttggaggac atttgtgaaa gtgttcgcaa gcgttttcaa taataaaaaa tgaaaaggag
     7621 taataaaaat gactgcattc gattcaaaag cgtatctgga caaggtggat gccttctggc
     7681 gcgccgccaa tttcatctcc gtgggacaac tgtacctgaa ggacaatcct ttgctgcaac
     7741 gcccgatcga ggcaaccgat gtgaagctgc atccgatcgg ccactgggga accatctccg
     7801 gacagaactt catctatgcc cacttgaacc gcgtcatcaa caaatatgac ctgaacatgt
     7861 tctacgtcga aggaccggga catggcggcc aagtcatggt ttcgaacgcc tatctggacg
     7921 gttcctatac ggaaatctat ccggacatca ccgaggatct ggaagggttg acgaaactct
     7981 acaaacaatt ctccttcccc ggcggggtcg cttcccatgc ggcaccggaa acacccggct
     8041 cgatccatga aggcggcgaa ctcggctatt cgctttctca cgcgaccggc gctatcttcg
     8101 acaatccgga agtgatcgcg gcgactgtcg tcggtgacgg ggaagctgaa accggtccgt
     8161 tggcggctgg ttggttctcc aatgtgttca tcaatccggt cacggatggg gctgtcttgc
     8221 cgatcctgta cctgaacggc ggcaaaatct ccaatccgac gatcctcgac cgtaaatcga
     8281 acgaagaatt gacgcagtac tttaacggca tgggctggga gccgttgttc gtggaaggga
     8341 ccgatccgga agcggtgcat ccgatcatgg cgcaagtgtt ggatacggct gtcgaaaaaa
     8401 tcaaagcgat ccaaaccgaa gcccgtaaag gctccgcagc cgaagcgaag atgccggttt
     8461 ggccggtcat cattttccgc accccgaaag gctggacggg tccgcaaaca tgggacggtg
     8521 aaccgattga aggcggcttc cgcgctcacc aagtgccgat tccggtggat gcgcatcata
     8581 tgcaacacat cgatgcgctg gtggattgga tgcagtccta ccgtcccgaa gaactgttca
     8641 ctgccgaagg ccaattggtg gctgaattga aagaaatggc accgaaaggc gaccgacgga
     8701 tggctatgaa tccggtcacg aacggcggta tcgatccgaa accgctcaat attccggatt
     8761 ggaagcagta tgccgtcgac acgaccgtac cgggcgcagt cattgcccag gacatgatcg
     8821 agttcggtaa cttcgcgcgc gacctggtcg tggacaatcc ggataacttc cgcatcttcg
     8881 gaccggatga aaccaaatcg aaccgcctga acaaagtctt cgaagtgacg aaccgcgtct
     8941 ggatggaagc gatcgatcct gcgtatgatg aatggctttc tccgtccggc cgtgtcatcg
     9001 attcgcagtt gtccgaacac caggccgaag gtttcctgga aggctatgtc ctgactgggc
     9061 gccatggttt cttcgccagt tacgaagcct tcctgcgcgt ggtggactcg atgatcaccc
     9121 aacatttcaa atggttgcgc aaagccaaag aacagacgtg gcgcaaacac tatccgtcct
     9181 tgaa
//
LOCUS       DAOMUJ010000018         9146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1921_length_9146_cov_2.819492, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000018 DAOMUJ010000000
VERSION     DAOMUJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_9146_cov_2.819492"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..351
                     /locus_tag="PLT73_01035"
     CDS             97..351
                     /locus_tag="PLT73_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM18997.1"
                     /translation="MRFVVARTFKKNGSAAIAIDAVPSIFGYSEELEQRFGRKIEVLL
                     LSGDSAEALEEAWPEYAPIAVTEDKESFERMIEEKVNRKK"
     gene            655..1566
                     /locus_tag="PLT73_01040"
     CDS             655..1566
                     /locus_tag="PLT73_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM18998.1"
                     /translation="MTLKQLRYMLAVAQYKSINEAAKKCFISQPSLSNAIKDLEEEIG
                     LELFFRTAKGITLTAEGSEFLKYAQQVIEQAELLEQRYLDKKPAKQLCSISTQHYAFA
                     VSAFVNMIKKSGADEYKFTLRETRTHEIIEDVKSFQSEIGILYLDSFNRNALQKILRE
                     NQLEFHPLFEAEPHIFVSVRNPLAAKQSVTLADLEDYPYLSFEQGEHNSFYFSEEILS
                     TVFHKKSILVSDRATLFNLVIGLDGYTISTGVLSEELNGTDIVSVPLDVEDRMVIGWI
                     ANKKAGISRQAAAYIKELKAVIRTYGN"
     gene            1768..2667
                     /gene="metF"
                     /locus_tag="PLT73_01045"
     CDS             1768..2667
                     /gene="metF"
                     /locus_tag="PLT73_01045"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRM18999.1"
                     /translation="MKIREMISKGEPTVSFEIFPPKSSYALETVFETLTELKDLHPDF
                     ISVTYGAGGTAQSNTVEIASRIKKDFGIESIAHLTGCTSTKEGMKRTLTSIKDSGIEN
                     ILALRGDIPQEKLADKNWNPEYRYASELIDDIKAAGDFSIGAACYPEGHVDSINKVDD
                     LKNLKRKMDHGADFMITQLFYDNDLFYQFKEKLDLVGIEQPVIAGILPVLNIKQVKRI
                     QEISGCNLPPKFLRILDRYAHDPASLQEAGIAYAVDQIIDLLSSGVDGVHIYTMNKPD
                     ATRRIMENISCVRKAVCRKEPQR"
     gene            2664..3329
                     /locus_tag="PLT73_01050"
     CDS             2664..3329
                     /locus_tag="PLT73_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRM19000.1"
                     /translation="MKTDLKLVAKYLGFGNKQPDERTKHDMERIAAQFEQVITGKWTY
                     GHYLLDHSVEGLITLEGTAVVLEGKSITRTLKRCKEVYIMVCTLGVQGDLLIERNKMM
                     SPTLGMIADACASAFVETIADSCQQEIESQLPAGFALTFRYAPGYGDLPLATNKTLLG
                     ELQSDKRIGVHLTDSMLMTPRKSIVAILGVLEEGISKGKNHRCGNTSCSECDLNDSCT
                     ARS"
     gene            3350..5731
                     /locus_tag="PLT73_01055"
     CDS             3350..5731
                     /locus_tag="PLT73_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRM19001.1"
                     /translation="MDIRKQIGEELLLFDGAMGTMLQAYGMKAGQNPEALNLEDPELL
                     GRIHREYVEAGAQFITTNTFGANAYKLEETGYSVTEVITAAVEIAKTATAGTEAKVAL
                     DIGPVGKMMKPIGLLDFDQAYDYFREQVMIGAAAGADLILIETMSDLAEMRAAVLAAK
                     ENSDLPIFATMTFTEDGNTLTGTEPEIMALSLDALGVDVLGVNCSLGPKELIPIIKRI
                     LAFTNTPVMVQANAGIPVTEAGVAKYNVVSKDYLEVAKELAGLGVSVIGGCCGTTPEY
                     IRDLQQAKASFVKREANKLKRYICSAQKKVCLDGQITLIGERINPSGNKGLKEQFVAG
                     NPLAAIKVAFEQVQKGADVLDVNTSLPMIDETDYMTKIIDGMSGLVEAPLQFDSTKPE
                     VLEAALRRYSGVAIVNSVNGKESSMAEIYPIVQKYGAFVVALCLDEAGIPDDADGRTK
                     VAQKLIERAEAYGIGAERLLVDCLVLTAAAQQKAVMETLKALRWVKENTKALTTLGLS
                     NVSYGLPFRALINRTYFAMALTSGLDTVIINPGADGIVDTMRAFNVLSGQDEGAIDYI
                     DYNNKKAVVKSDGPKQENVVRSYREMLLEGDEAGIMKATNALLKDNTPLAVVDDHIVP
                     ALDEVGKLYEQKNIFLPQLIRAAETAGKAFETIRAKLAGGNEKLESKGTIVMATVRGD
                     IHDIGKNLAKVLLENYGYDVIDLGKDVPIEEVVAVAKQHQVKLVGLSALMTTTVTAME
                     DTISALREADLSVKVFVGGAVLTEKYAKAIGADYYCKDARAGVTVAETVFGQA"
     gene            complement(5789..7027)
                     /locus_tag="PLT73_01060"
     CDS             complement(5789..7027)
                     /locus_tag="PLT73_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004908176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRM19002.1"
                     /translation="MENKWMRAAIPALLIHCSIGTVYCWSLLKGGIAEYIGRPKSEVE
                     WAFSLAIFCLGMSAAFAGKIVERDIHKASLIAAVCFAAGMAGTGFFIQQKSLIGIFLS
                     YGVLMGIGCGIGYLSPVKTLMLWFKNNKGLATGIAVAGFGLAKVIASPIIEMLLGATN
                     SDGTLADPSRLYKLFYILAVVYFIMMFAGHLLLKKPADWVEVSAQAKGTHTLDIFKNK
                     TFIGIWLMFYLNITCGLALISQEKDLLHSIGFGAIATISSLTAIFNAGGRLGFSAFAD
                     RLKDRNTIYIWIFGLSIGATTITLLANGVDNAIAPLIILLLCVINAGYGGGFSSLPPL
                     LADRFGMETISTVHGLTLSAWALAGLSGNQLSALIIAKTGSYNNVLYAILAMYAVAFF
                     ISAFLLRAKSDQKKRHPVHH"
     gene            7272..8405
                     /locus_tag="PLT73_01065"
     CDS             7272..8405
                     /locus_tag="PLT73_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRM19003.1"
                     /translation="MKVEIRKADLQDLVALKIIEDACFPEAEAATLASLSERLKLFPE
                     SFLVAEVEGELVGFVNGAVIDEPIIRDAHYHDAGLHNPEGAYQSVFGLDVDPAFRRQG
                     IAEKLLMALIDASRKAARKGLTLCCKEEKIPYYEKFGLVNSGKSSSTHGNAVWYDMIL
                     HFEEERVEQMNPKIILASQSPRRSELLSLCIKNFAVQAADIDEKAIEERILAEAGQDP
                     FVETATELVETLAREKAAVIHRENSEAMVIGSDTVVMLDEKILGKPVDEADAYAMLRA
                     LAGKTHSVLTGVSILWGEKEETFASETKVSFFEWDDRMEAEVKAYVASGKPMDKAGAY
                     GIQEEAALWVSGIEGDYNTIVGFPVALVDKAIHRLLTEAQTEK"
     gene            complement(8480..>9146)
                     /locus_tag="PLT73_01070"
     CDS             complement(8480..>9146)
                     /locus_tag="PLT73_01070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM19004.1"
                     /translation="TISYWKNQKKREMKVKYKNDTVKYDEIQSLKIELSEKEKMIAGE
                     PYLAGDAELVALRQNARENMRAFNDEIDPRKRSELLKNWFGKTGQKIYMEPSLSLDYG
                     SNIFVGENFYANFNCTILDTCPVTFGNNVMIAPNVSFYTATHPIDPVERNSGTEYGKP
                     ITIGDNVWIGGSSVIGPGVTLGDNVVVGMGSVVTKSFPDNVVIAGNPARVIRQIELSE
                     AKS"
BASE COUNT         2445 a         2170 c         2478 g         2053 t
ORIGIN      
        1 atcagcagga tgggaacagg aatgggaatg cgaacgcatc attttctttt accaagaatg
       61 cggtctgttt gctataatgg actttgaggt gaattcatga gattcgtagt ggcaaggaca
      121 ttcaaaaaga acggcagtgc ggcaatcgca atcgatgcag tgccatccat ttttggctat
      181 tcggaggaat tggagcaacg attcggccgg aaaatcgagg tgctcctcct gagcggggac
      241 tccgcggaag ccttggaaga ggcgtggccg gagtacgcac cgattgcggt gacagaagac
      301 aaagaaagct tcgaacgcat gatagaagaa aaagtcaacc ggaaaaaata gcatcggatc
      361 ggcttggcag cacatggaaa tgctcccaag ccggtttttt tcgattttac tccggtgaac
      421 gcttgcctaa ccggagcaga gacttcagat tcgccctcaa ctgcggcgaa aactgcctgg
      481 ccggagcagc cggcccaaaa tccgacattc ctccggtgaa cgctatcttc accggagcag
      541 gcaccgtttt ttcgatgcgc atcagcccgt ccttggccgt gcgggaaccc atctggtata
      601 atcggagcag aatcaaaaaa gcatgccaaa gcaacgagag gagaggcaag aaacatgacc
      661 ttaaaacaat taagatacat gctggcagtc gcccaataca aatcgatcaa cgaggccgcc
      721 aaaaaatgct tcatttcaca accgagcctc tcgaatgcca tcaaggacct ggaggaagaa
      781 atcggcctgg aactgttctt ccggacagct aagggcatca cgctcacggc tgaaggcagc
      841 gaatttctga agtatgccca acaggtgatc gagcaagccg aattgctgga acagcgttat
      901 ctcgacaaaa aaccggccaa acagttgtgt tccatttcga cccaacatta cgcgttcgct
      961 gtcagcgcat tcgtgaacat gatcaaaaag agtggagcgg atgaatacaa gttcacgctg
     1021 cgtgagacac gcacccatga aatcatcgaa gacgtcaaga gtttccagag tgaaatcggc
     1081 atcctctatc tggattcgtt caaccgcaat gcactccaaa aaattttgcg ggagaatcaa
     1141 ttggagttcc atcctttgtt cgaagcagaa ccgcatatct ttgtgagcgt ccgcaatcca
     1201 ttggcggcca aacaaagcgt taccttggcg gatctcgagg attaccccta cctgtcgttc
     1261 gaacagggcg agcataattc cttctatttt tcggaagaga tcctcagcac cgttttccat
     1321 aaaaaaagca tcctggtcag cgaccgggca actttgttca acttggtgat cggccttgac
     1381 ggctacacta tctccaccgg tgtacttagt gaagagctga acggaacaga tatcgtttcc
     1441 gtacctttgg atgtagagga ccggatggtg atcggttgga tcgccaacaa gaaagccggg
     1501 atcagccggc aggcggccgc ctacatcaaa gagctgaagg cagtgatccg gacctatggg
     1561 aattaaaagg tccggtctat tttgggtata gtcttttgct ataactaaat atattttttt
     1621 ggtgttactc tatatcttca ttccatggca tactattgga aataaatgac ttctttcggg
     1681 aagtcatcaa ctgacatgat caatcataca caaagtaagt gcgcttgaaa aattgccact
     1741 ttgaacaaat tgagggagga catggttatg aagattagag agatgatcag caaaggggaa
     1801 ccgaccgttt catttgaaat ttttccgccg aaaagcagct atgcccttga gacggtgttc
     1861 gagaccttaa ctgaattgaa ggacctgcat cccgatttta tttccgtgac ctacggcgct
     1921 ggcgggacag cccaaagcaa cacggtcgaa atcgcatcgc gtatcaaaaa ggatttcgga
     1981 atcgaatcga tcgcccattt gaccggctgc acctcgacaa aagagggcat gaaacggaca
     2041 ctgacatcga tcaaagacag cggaatcgaa aacatcctgg ctttgcgtgg ggatatcccg
     2101 caggaaaaat tggcggacaa aaattggaat ccggaatacc gttatgcttc cgagctcatc
     2161 gacgacatca aagcagcagg cgatttttcg atcggagctg cctgctatcc ggaaggacat
     2221 gtcgattcca tcaacaaagt ggatgacctg aaaaatctga aaaggaaaat ggaccatggc
     2281 gctgacttca tgatcacgca gcttttctac gacaatgatc ttttctatca gttcaaggag
     2341 aaactggatc ttgtcggcat cgaacagccg gtcatcgccg ggatactgcc ggtgctgaac
     2401 atcaaacagg tgaaacggat ccaggaaatt tccggctgca acctgccgcc gaaatttctg
     2461 cgcatacttg atagatatgc gcacgatccg gcatcactgc aggaagccgg tattgcttat
     2521 gcagtcgatc agatcatcga cctgctatct tcaggtgtgg atggtgtgca catctacacg
     2581 atgaacaagc ccgacgcgac aagaagaatc atggagaaca tctcctgtgt acgcaaggcg
     2641 gtctgcagaa aagagccgca aagatgaaga cggatctgaa gctggtcgca aagtatttgg
     2701 gcttcggcaa caaacaaccg gacgaacgga caaagcacga tatggagagg atcgccgctc
     2761 agtttgagca ggtgatcact gggaagtgga cttatgggca ttatcttctg gaccactccg
     2821 ttgagggact gatcacgctg gaggggaccg cagttgtgct ggaagggaaa agcatcaccc
     2881 gcacgctcaa gcgatgtaaa gaggtctaca tcatggtctg tacattaggc gtgcagggag
     2941 acctcctcat cgagcgcaac aaaatgatgt cgccgacctt gggcatgatc gctgatgcct
     3001 gtgcatccgc attcgtcgag acaattgcgg acagttgcca gcaggaaatc gaaagccagc
     3061 tgccagcggg attcgcattg actttccgtt acgcgcccgg atacggggat ctgcctttgg
     3121 ccaccaacaa aacgctgcta ggggaattgc agtcagacaa acggatcggg gtccatctga
     3181 cggattcgat gctgatgacg ccaagaaaat caatcgtggc gattttaggc gttctggagg
     3241 aaggcatctc caaaggcaag aatcatcgct gcggaaacac aagttgcagc gaatgcgatc
     3301 tgaacgacag ctgcacggcc agaagttaag ctagggggga caaaaaataa tggatatcag
     3361 aaaacaaatc ggcgaagaac tgcttctctt cgatggagcg atgggtacga tgctccaggc
     3421 atacgggatg aaagcaggcc aaaatccgga agcgttgaat ctggaagatc ccgagctgct
     3481 gggccggatt catcgggagt acgtcgaagc cggtgcgcag ttcatcacga caaatacttt
     3541 tggtgcgaat gcctacaaac tggaggagac cggttactcc gttacggaag tgatcactgc
     3601 tgctgttgaa atcgccaaaa cggcaacggc agggaccgag gcgaaagtgg cgctcgatat
     3661 cggaccggtc ggaaagatga tgaaaccgat cggattgctt gatttcgacc aggcttacga
     3721 ttacttccgc gaacaagtga tgatcggggc tgcggccgga gcggatctga tcctcatcga
     3781 aacgatgtcg gatctggcgg aaatgcgtgc ggccgtgtta gcggcgaaag aaaacagcga
     3841 cctgccgatc tttgcgacga tgacctttac ggaggacggc aacactttga ccggaaccga
     3901 gccggaaatc atggcgctct ccttggatgc attgggtgta gatgtgctgg gcgtaaactg
     3961 ctcattgggg ccaaaggaat tgattcccat catcaaaagg attttggcct tcacgaatac
     4021 gccggtgatg gtgcaggcca atgccggtat ccctgtgacg gaagctgggg tcgccaaata
     4081 taacgtcgtt tccaaggatt accttgaggt ggccaaggaa ttggccggtt tgggggtctc
     4141 cgtgatcggc gggtgctgcg gcacgactcc ggaatacatc cgtgatctgc agcaggccaa
     4201 ggcgtctttt gtgaaaagag aagccaataa attgaagcgg tacatctgct cggcgcagaa
     4261 aaaagtttgc ctggacggcc agatcaccct catcggggag cggatcaacc cgtcaggcaa
     4321 caagggtttg aaggaacaat tcgtggcagg caatcccttg gcggccatca aggtcgcttt
     4381 tgagcaggtc caaaaaggcg cggatgtttt ggacgtcaac acctctttgc cgatgatcga
     4441 cgaaacggac tatatgacaa aaatcatcga tggcatgagc ggtctagtgg aagcgccgct
     4501 ccagttcgat tcgaccaaac cggaagtgct tgaagcagct ttgaggcgct acagcggtgt
     4561 cgcaatcgtc aattcggtca acgggaaaga atcctcgatg gccgaaatct atccgatcgt
     4621 ccagaaatac ggcgcctttg tggttgcctt gtgtctggat gaagcgggca tcccggatga
     4681 cgctgacggc cggaccaagg tcgcccagaa attgatcgag cgagcggaag cgtatggcat
     4741 cggtgcagag cgcttgttgg tggattgcct tgtgctcact gcggcagccc agcagaaagc
     4801 ggtcatggag acgttgaagg cattgcgttg ggtcaaggaa aacacgaagg cgttgactac
     4861 tttggggttg agcaacgttt cctatggact gcctttccga gccttgatca accgaaccta
     4921 ttttgcgatg gccctgactt cgggattgga tacggtcatc atcaacccag gggccgacgg
     4981 aatcgtcgac acgatgcgcg ccttcaatgt tctttcaggg caagacgaag gtgccatcga
     5041 ctatatcgac tacaacaaca aaaaggctgt ggtcaaatcc gatggtccca agcaggaaaa
     5101 cgtggtacgt tcctaccggg aaatgctgtt ggagggcgat gaagctggga tcatgaaggc
     5161 aaccaacgcg ctgcttaaag acaatacgcc attggctgtc gtggacgatc acatcgtgcc
     5221 ggcattggat gaagtcggca aactctatga gcagaaaaac atcttcctgc ctcagctgat
     5281 ccgcgccgcc gaaacggccg gtaaagcctt cgagacgatc cgtgcaaaac ttgctggagg
     5341 caatgagaag ctggaatcga agggaaccat cgtgatggct accgtaagag gggatatcca
     5401 cgatatcgga aagaatctgg caaaagttct gcttgaaaac tatggctacg acgtcatcga
     5461 cctgggcaaa gatgttccga tcgaagaggt tgtggcggtc gccaagcaac atcaagtcaa
     5521 attggtagga ctttctgcgt tgatgacgac gaccgtcact gcgatggagg acacaatctc
     5581 ggccctgcgc gaggcggatt tgtctgtgaa ggtcttcgtc ggaggagccg tactgacaga
     5641 aaagtacgcc aaagccatcg gcgcggatta ctactgcaaa gatgcccgag ccggtgtcac
     5701 agtagccgaa acagttttcg gacaagctta aaaaaatccg gcgacgagtc atacgtgatg
     5761 acttgtcgcc ggatttttat ttgttgtgtc agtggtggac gggatgtctc tttttttggt
     5821 ccgatttggc ccgtaacagg aaagcgctga tgaagaaagc gacagcatac atcgccaaga
     5881 tggcatacaa gacattgtta tacgagccgg tcttcgcgat gatgagggcg ctcagctggt
     5941 taccggacaa accggcaaga gcccaagccg ataaagtcag tccatggaca gtactgatgg
     6001 tctccattcc gaagcggtcc gccaatagcg gtggaagact tgaaaaacca ccgccgtaac
     6061 cggcgttgat gacacatagc aacaaaatga tcagcggcgc gatggcatta tccactccgt
     6121 tcgctaaaag cgtaatcgtc gtagctccaa tcgatagccc gaaaatccaa atataaatgg
     6181 tattccggtc ctttagccta tcggcgaacg ccgaaaatcc gagccttccg ccggcattga
     6241 aaatcgcagt caaggaactg atcgtggcaa ttgcgccaaa accaatggaa tgaagcagat
     6301 ccttttcctg ggagatgagt gccagtccgc aagtgatgtt gagataaaac atcagccaga
     6361 tgccgatgaa tgtcttattc ttgaagatat caagcgtatg ggtgcctttt gcctgtgcgc
     6421 tcacttcgac ccaatccgcc ggcttcttca aaagaagatg acccgcgaac atcatgatga
     6481 agtagacaac agccaggatg tagaatagct tgtagagacg agaaggatca gcgagtgtac
     6541 cgtctgagtt tgttgcaccc agcaacatct cgatgattgg gctggcgatt actttggcca
     6601 acccgaaccc tgcaaccgct attccagtgg cgaggccttt gttattcttg aaccaaagca
     6661 tcaaggtctt taccggcgaa agatatccga taccgcaccc gatgcccatc aaaacgccat
     6721 agctcaggaa aatcccgatc agcgactttt gctggatgaa aaagccggta ccagccattc
     6781 ctgctgcgaa gcacacagct gcaatcaagg aagccttatg gatatccctt tcgacgattt
     6841 taccggcgaa agctgccgac atcccgagac agaaaattgc cagactgaag gcccattcta
     6901 cctcgctttt cggccttccg atatattcgg cgattcctcc tttcagcaaa gaccaacagt
     6961 agactgtgcc gatgctgcaa tggatcagca gtgccgggat agcggctcgc atccatttat
     7021 tttcgatatt gttcattttg aagcccccct taggtaatga taatatggat tgcgtgaata
     7081 ttttatcaag ctatcgtaat catactctcg atccgcaggg aatgcaatcg ctttcccgaa
     7141 cagtgagaga gggcggtttg tctaatccgg acaatgtttt ttccctagga ggccgcccag
     7201 atggaattct ctggaaaaat cgaaaatttg tcggtaatat gaactatgta cagaaaggga
     7261 ggaatcagcg gatgaaagtt gagatcagga aagcggatct tcaggatttg gttgcattga
     7321 aaataattga ggatgcgtgt tttccggaag ccgaggcagc gacgctagca tcactcagcg
     7381 agcggctgaa actgttccct gaaagcttcc ttgttgccga agtcgagggc gaattggtgg
     7441 ggttcgtgaa cggggcagtc atcgatgaac cgatcatccg cgatgcccac taccatgatg
     7501 ctggcctgca taatccggag ggtgcttatc aaagcgtatt cggtttggat gtggatccgg
     7561 ctttcagaag gcagggaatt gcggaaaagt tgcttatggc actgatagat gccagcagga
     7621 aagcggccag aaaaggcttg acgctttgct gcaaagaaga aaagatccct tattatgaga
     7681 aattcgggtt ggtgaacagc ggcaaatcaa gttcaacgca tgggaatgct gtttggtatg
     7741 acatgatttt gcactttgaa gaagaaaggg tagaacaaat gaatccgaaa atcatattgg
     7801 cgagccaatc gccaagacgc agcgagttgc tgtccttgtg cataaagaat ttcgcggtcc
     7861 aggctgcgga tatcgacgaa aaagcgatag aagaacgcat cctcgccgaa gcgggacagg
     7921 atccgttcgt tgaaacggcc actgagctgg tggaaacgtt ggcgcgggaa aaagcggcgg
     7981 ttatccaccg tgagaacagc gaagcgatgg tcatcggctc cgacactgtg gtcatgttgg
     8041 acgaaaaaat cctcggcaaa ccggttgatg aggccgatgc ctatgcgatg ttgcgcgcac
     8101 tggctggtaa gacccactcg gtcctgaccg gcgtcagcat tttatggggc gaaaaggaag
     8161 agacctttgc ttctgagacg aaagtcagct tctttgagtg ggatgatcgc atggaggcgg
     8221 aagtgaaggc atatgtggca tcgggcaagc cgatggacaa agccggagca tacggcatcc
     8281 aagaggaagc tgcgctttgg gtttccggga tcgaagggga ctacaatacg attgtcgggt
     8341 ttccagtcgc cttggtcgat aaagccatcc atcggttgct gacggaagca cagacagaga
     8401 agtagttgtt gaaagcaaag aaacgatcgc gctcagctgg atgcagctta cgcgatcgtt
     8461 ttttttttta ctcgtgtatt taggacttgg cttcggaaag ttcgatttgg cggatgacgc
     8521 gcgccgggtt ccctgcaatc acgacattgt caggaaaaga tttagtcacg acactgccca
     8581 taccgacgac aacattgtcc cccagcgtga cccccggacc gatcacggaa gagccgccga
     8641 tccagacgtt gtcaccgatc gtgatcggtt tgccgtactc cgttccactg ttccgttcga
     8701 ccggatcaat cggatgggtg gccgtataaa agctgacgtt cggtgcgatc atcacgttgt
     8761 tcccgaatgt gaccgggcag gtgtccagta tcgtgcaatt gaagttcgca tagaaattct
     8821 cgccgacaaa gatattggaa ccgtagtcca atgaaagcga cggctccatg tagattttct
     8881 ggccggtctt cccgaaccag tttttcaaca gctcgctgcg cttgcggggg tctatttcgt
     8941 cgttgaatgc gcgcatgttt tcacgtgcgt tctgccttaa ggcgacgagc tccgcatcac
     9001 cggccaaata gggttctccg gcaatcattt tttctttttc tgacaattct atctttaaac
     9061 tctgtatttc atcatatttt actgtgtcat ttttatattt tactttcatt tctctttttt
     9121 tctgattctt ccaatatgat atcgta
//
LOCUS       DAOMUJ010000019         8939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_1989_length_8939_cov_2.698672, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000019 DAOMUJ010000000
VERSION     DAOMUJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_8939_cov_2.698672"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="yycI"
                     /locus_tag="PLT73_01075"
     CDS             complement(<1..860)
                     /gene="yycI"
                     /locus_tag="PLT73_01075"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HRM19005.1"
                     /translation="MDFRRIKIIFILTFAALNIYLLSVLLEKNETTLSFGSESNTLNI
                     EEAMKADDISFPTLSTNQEKIPLLKTDKSRILESDQSKLENQTTVFEDGVLFSTLSET
                     IPLEVDDQNLTIVDRTPITDFILQGNVLHGTEYSFLTYLPLRRQLVYAQVANNIPIGD
                     STGSIIFNLDPDYEVISYEQTFAGTAEVQGNSRTVISQKRAVEALYLNNQIPANATVR
                     TVQLTYYRTLSLSDMDIYSPMWYIELEIKNAPVEIRRVDALTGSIITTPSVTLPSVEA
                     QVKAKTATNSA"
     gene            complement(850..2166)
                     /gene="yycH"
                     /locus_tag="PLT73_01080"
     CDS             complement(850..2166)
                     /gene="yycH"
                     /locus_tag="PLT73_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HRM19006.1"
                     /translation="MKHKESPMLSALLYTLVAISLFLTVRILSTPSTVGLSQNSATSV
                     SANLTNTKKVEDVFAPIRMIVHTDKRMYLTQMPEIMRSVNELLSESVFNGIEGVSTYT
                     KEEYDALILSPTQLEIRFAEEVPLELLSRYFTNLQEDYYGESIGRIIFNSKEEDPVYL
                     LNDETKQVFTVARPANLLQSLAELYESQKEAYAEVESYETELAISYLPIEAITIQKLV
                     YLVEKPSNSYFIDLLFDDTTDLKDNGSDEFVSYSDNISELSIDKETGQLSYYRNILDA
                     EDLPDYRLIRDSFHEIKMLDNWTNPFYFYGFDKENDNVYYRRYVNGYPIFGEVDYGLT
                     RIRMSGSSMTELQFLTQVIQTPLTDRGEDVTLLSGQDLLAALNAGGYSSQEIQMIALG
                     YDWTFSEESNRLVNLTPKWFIKIDGVWKALDQWIGGTETEADDSGF"
     gene            complement(2163..4010)
                     /gene="walK"
                     /locus_tag="PLT73_01085"
     CDS             complement(2163..4010)
                     /gene="walK"
                     /locus_tag="PLT73_01085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005918544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HRM19007.1"
                     /translation="MNKKIRLVHSIHFKIPMLFILLLLVSLQLIGAYFIRELETKMIS
                     NFDSQMSLNAGFLEKTLQPILMADDTEKLEESVQTILSDFTGANILETQVMDEQGYIL
                     GINDQTLQSLIGTKSTDRDVQQVILLDAPSSYQYVKEDTNSRVLKNISPIYSMDNTGT
                     LIGVLVMESNIESVYSQMSQTVSIFLNASVVAIIITIVLAVIISRGITRPISEMRRQT
                     ANIADGDYSGKVTVYGADELGELAETINDLSYKVKDAQETTESERQRLDSVLRHMTDG
                     VLATDRRGKIIIINSRAMDLLSISQDKAIGQSIMKVLKLGEKFTFRQLLETQDELILN
                     IPTDDQDTILRGEFSVIQRESGFISGLVCVLSDIMEQEKVEQERRSFVSNVSHELRTP
                     LTSVKSYTESLIDGAWEDEEIAPEFLKVISTETDRMIRMITDLLNLSRMDQGKQDLNR
                     EFVSINELVAHIIDRFEMVLKSEQYRNKKYTIERDFTQRTLWVEIDQDKFIQVIDNIM
                     NNAIKYSPDGGKITCKLMETHNSVVISITDEGLGIPRKDIGHVFDRFYRVDKARARSM
                     GGTGLGLAISKEVVQLHGGKIWVTSVENKGSTFFISLPYIPMDEEDEWG"
     gene            complement(4017..4724)
                     /gene="yycF"
                     /locus_tag="PLT73_01090"
     CDS             complement(4017..4724)
                     /gene="yycF"
                     /locus_tag="PLT73_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HRM19008.1"
                     /translation="MKKVLVVDDEKPISDIIKFNLTKEGYDVFTAFDGEEALAQFAKV
                     GPDIILLDLMLPKMDGLEVCREIRKTSDVPIIMLTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRQASPVVAPPEEDDTNEIKIGALIIHEDAYIVSKRGVEIELTH
                     REFELLHYLAKHIGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDTPSHPTW
                     LITRRGVGYFLKNPDQE"
     gene            complement(4935..6311)
                     /locus_tag="PLT73_01095"
     CDS             complement(4935..6311)
                     /locus_tag="PLT73_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM19009.1"
                     /translation="MKKKPIIIGATAILLVVAGYFGAGSYYKDKFLPNTMLDGIDISN
                     DTVAQAKAETTAAIAQTQIQIVENGENIYAFSPADLGVSIDNTEKLETMKAEQNVWNW
                     PILLFKEKQAETDLSGVVVDETALADILAAMPLENEVRTPPVNATVVKATEGYEISPE
                     TTGNKVDLELLKQEMLEAIIAGETEIDLAQTYQQPTLTADDPVLTETLQKLNDLTDTV
                     IQYSISGQTETVPQTAITEWLGVDENGDVSVNREGVAAYLQGLSDRYSTYSRTRDFLA
                     TDGETVQVPSGTYGWTLAVAAETDKLVNYVLAGEDVTVTPNYNGTGYHADGADIGTTY
                     VEVDLSSQHMWYYKDGAVVLETDIVSGHPMSPTPTGVFYIWNKEEDAVLKGYNPRTEK
                     DYESPVEYWMPVDWTGIGIHDSSWQPAYGGEYWLTAGSNGCVNTPPGVMAELFGMIGI
                     GVPVVIHS"
     gene            complement(6425..6709)
                     /locus_tag="PLT73_01100"
     CDS             complement(6425..6709)
                     /locus_tag="PLT73_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19010.1"
                     /translation="MKENGPNSGKRPPAGADNVTIDRNSEEAVFIGERTQKVVKLRRS
                     LRSPEENIEVPPISARLEHCGASESGVCLFAFLPYWYYDECLRSILLSKN"
     gene            complement(6784..7512)
                     /locus_tag="PLT73_01105"
     CDS             complement(6784..7512)
                     /locus_tag="PLT73_01105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRM19011.1"
                     /translation="MALNEMMDRPVVKKELVAFMRDRLKRFPGELGELEKTANERGVP
                     IIPHETAVFLNMLLGQIKPKQILEIGAAIGFSGSLMAQHVGAEGHVTTIDRFEVMINH
                     AKANFEKLGITDKVTLLEGDAAEVLPTLEGPYDFIFMDSAKSKYYEFLPHCIRLLKKE
                     GMLVIDDVFQGGTILDDEKDVPKRVRKIHRRLNKLMDTVLDHPALETSLVPLGDGLLL
                     IVKKEDFDFGYILDEIKAEKQNQA"
     gene            complement(7583..8917)
                     /locus_tag="PLT73_01110"
     CDS             complement(7583..8917)
                     /locus_tag="PLT73_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM19012.1"
                     /translation="MNPDPEGQSLLSKIALILTLTLLNAYFASAELAFVSINRSKIEK
                     LAKDGDKKSQKVLRLLENPDDFLATIQVAITFAGFFSSASASSNFVGYIEPLLGNIAG
                     GKTIAMIIITIILSYITLVFGELYPKQIALQMPESIARHTAGPIVVVKTLFKPFVWLL
                     SFSTGILKKLTPIEFTNEEEKLTRDEMKAILAQSRGEGVIDPSEFTMMQGVLSLDTKL
                     ARELMVPRTDTLMMDLDDDLEYNIALVLNSQYSRIPVYEEDKDNVIGVLHMKNVLKAS
                     KEVGFERISFRELVNTPLFVPSTIYIDDLLVEFRKTHTHMAIIKDEYGGGEGIITLED
                     LLEEIVGDIEDEYDEIYRLSKKIDDNHYVVNGRMPLDKFNQLFKTAIASEEVDTIAGY
                     MIEELGYFPKDDEHATIQTGEYLLTTTQVESGRIRGIRVTREPVQTAVVDDE"
BASE COUNT         2158 a         2305 c         1950 g         2526 t
ORIGIN      
        1 gcactattgg tggctgtctt cgccttgact tgcgcttcga ctgacggtag cgtgacggaa
       61 ggcgtcgtga tgatactgcc ggtcaacgca tccacccgtc tgatttctac aggtgcattt
      121 tttatttcaa gctctatgta ccacattggg ctgtagatat ccatatccga tagactcagc
      181 gtacggtaat acgtcaattg taccgtccgg acagtcgcat tcgctggaat ctgattattg
      241 aggtacaagg cttcaaccgc gcgcttctgt gaaatcacag tccggctgtt gccttgtact
      301 tctgccgttc ccgcaaatgt ctgttcatag gaaatgactt cataatcggg atctaagttg
      361 aaaatgatgc tgcccgtgct gtcgccgata ggaatattgt ttgcgacttg ggcgtacaca
      421 agttgtctcc tgagcggcaa atacgtaaga aaagaatatt cagttccgtg gagtacattc
      481 ccttgcaata taaaatccgt gattggtgtt ctgtcgacaa tcgtcaaatt ttgatcgtca
      541 acctccagag gaatcgtctc agataaggta ctgaacaaca caccatcttc aaagactgtc
      601 gtctgattct ccaattttga ctgatcggac tccaaaatgc ggctcttgtc cgtcttcaac
      661 aaaggaattt tctcttgatt ggtcgataaa gtcgggaaac tgatatcatc ggccttcatt
      721 gcctcttcga tgttcagcgt gttgctctcg cttccgaagc tcaatgttgt ctcgtttttc
      781 tccaacagga cactgagcaa atagatgttc aaggctgcaa aggtgaggat aaagatgatt
      841 ttgatacgtc taaaatccac tatcatccgc ctccgtttcc gttccgccga tccattgatc
      901 cagcgctttc catacgccgt cgatcttgat aaaccattta ggcgtcagat tgacgagccg
      961 gttggattct tcactgaaag tccaatcata gcccaaagcg atcatctgaa tttcttggct
     1021 actgtagcca ccggcattca gcgcggccaa aaggtcttgc ccgctcaaca gcgtgacatc
     1081 ctctccacga tccgtcagcg gtgtctggat gacttgcgtc aggaattgca attccgtcat
     1141 ggatgatccg gacatacgga tccgcgtcaa tccataatcg acttcaccga aaatcggata
     1201 gccattcaca tatctgcgat aataaacatt gtcgttttcc ttgtcaaacc catagaaata
     1261 aaaaggattc gtccagttgt ccaacatctt gatttcatgg aaactgtcgc ggatcaaacg
     1321 ataatcaggc agatcttcgg catccaggat gttgcggtaa taactcaact gcccggtttc
     1381 cttgtcgatg ctcaattcgg agatattatc gctgtagctc acaaattcat cgcttccgtt
     1441 atccttcaga tcggttgtat catcaaaaag caagtcaatg aaataactgt tggaaggctt
     1501 ctcaaccaga tagaccaact tttggatcgt aatcgcctct atcggaagat aggagatggc
     1561 taattcggtt tcatacgatt cgacttctgc gtaggcttcc ttttggctct cgtaaagttc
     1621 cgccaacgac tgcagcagat tagccggacg tgccactgtg aacacttgct ttgtttcatc
     1681 attcaaaaga tagacgggat cttcttcctt ggaattaaag atgatccggc cgatactttc
     1741 gccgtaataa tcttcctgta gattcgtgaa gtaccgcgaa agcagctcca acggcacctc
     1801 ttcggcaaaa cggatttcca attgcgtcgg tgagagaatg agcgcatcat actcctcctt
     1861 cgtataggtg gaaacgccct cgattccgtt aaacacactt tcactcagca attcattcac
     1921 acttcgcatg atttcgggca tttgcgtcaa atacatgcgc ttatccgtgt ggacgatcat
     1981 cctgatcggt gcaaagacat cctctacttt cttcgtattg gtcagattgg cagagaccga
     2041 ggtcgctgaa ttttggctca aaccgaccgt actcggagtg gaaaggatcc ttaccgtaag
     2101 gaatagactg attgcaacca aggtgtacaa aagcgcgctc agcatcggtg attctttatg
     2161 tttcatcccc actcatcctc ctcatccatc ggaatgtaag gcaaggaaat aaagaaagtc
     2221 gatcctttat tttccacact ggtcacccag atttttccgc catgcaattg aacaacctct
     2281 tttgaaatgg ccaacccgag cccggtgccg cccatcgaac gcgcacgggc cttgtccacc
     2341 cgataaaatc ggtcaaagac atggccgata tctttgcgcg ggatgcccaa accttcatcg
     2401 gtgatactga tgacgacact gttatgcgtc tccatcaatt tacaggtgat tttcccaccg
     2461 tccggagaat atttgatggc attgttcatg atattgtcga tcacctggat aaatttatcc
     2521 tgatcgattt cgacccacag cgtgcgctgc gtaaagtccc gttcgatcgt gtatttttta
     2581 ttgcggtact gttccgactt cagcaccatc tcgaagcgat cgatgatatg tgcaaccaat
     2641 tcgttgatgc tgacgaactc ccgattgaga tcttgtttcc cttggtccat ccgggataag
     2701 ttcagcagat cggtgatcat ccggatcatc cgatctgtct ccgttgagat gaccttcagg
     2761 aattcgggag cgatttcttc atcttcccag gcaccgtcaa tcagtgattc cgtgtaactt
     2821 ttcacgctcg tcaacggtgt ccgcagttca tgggaaacat tcgaaacgaa gctgcggcgt
     2881 tcctgttcga ccttttcctg ctccataata tcggaaagca cacacaccaa cccgctgatg
     2941 aatccggatt cccgctggat gacagaaaat tccccgcgca ggattgtgtc ctgatcatct
     3001 gtcgggatgt tcaggatcaa ttcatcctgt gtctccaaca actggcggaa agtgaatttc
     3061 tcgcccagtt tcagcacctt catgatcgat tgtccgatcg ctttatcttg tgaaatcgag
     3121 agcagatcca tcgcacggct gttgatgatg atgattttcc cgcggcgatc ggtggccaac
     3181 acgccatccg tcatgtgccg cagaacgctg tccaaccttt gacgctccga ttctgttgtt
     3241 tcctgggcgt cctttacttt ataagacaga tcattgatgg tttcggccaa ttcgcccaat
     3301 tcatccgctc catagacggt cactttgccg gaataatccc catcggcaat attcgccgtc
     3361 tgccggcgca tttcggatat cggacgcgtg atcccccggg agatgataac agccaacaca
     3421 atcgtgatga tgatcgccac gaccgatgca ttcaaaaaga tgctcaccgt ctggctcatc
     3481 tgcgaataga ccgattcgat gttagactcc atcaccaaca cgccgatgag ggtacctgta
     3541 ttgtccatcg aatagatcgg actgatgttc ttcagcacac gactattcgt gtcttccttc
     3601 acatattgat aggaagaggg ggcatccaga agaatgactt gttggacatc ccgatcagtc
     3661 gacttcgttc cgatcaagga ttgtaacgtt tggtcattga ttccgagaat ataaccctgt
     3721 tcatccatga cctgcgtttc caggatgttg gcgcccgtaa aatcgctgag gatggtctgt
     3781 acggactctt ccagcttttc cgtatcatca gccatcagga tcggttgcag cgttttctcc
     3841 agaaagcctg cattcaagct catctggcta tcgaagttgc tgatcatttt cgtttccagt
     3901 tcacggataa aataagcccc gatcagttgc agggatacca acaggaggag aatgaacagc
     3961 attggtatct tgaagtgaat ggagtgaacc agacgtatct ttttgttcat atataattac
     4021 tcctgatccg gattcttcaa gaagtagcct actcctcttc gcgtgatcaa ccaagtcgga
     4081 tggctcggtg tatcttcaat tttttcacgc agtctgcgga ctgtcacatc aacagtgcgg
     4141 acatcaccga aataatcata tccccatacg gtctgcagca aatgttcccg cgtcatcact
     4201 tgaccgatgt gtttcgccaa ataatgcaat aattcgaatt cacggtgagt caactcgatc
     4261 tctactccac gcttcgacac gatgtacgca tcttcatgga tgatcaaagc gccgattttg
     4321 atttcattcg tatcgtcttc ttcaggcggt gcaacgaccg gagaggcttg tctcctcaag
     4381 tttgctttta cacgtgcgac taattcccgg ttcgaaaatg gcttcgtgac atagtcatcc
     4441 gcacccaatt caagtccgag cactttgtcg atttcagagt cttttgccgt caacatgatg
     4501 atcgggacat cgctcgtctt gcggatttcc cggcagactt ccaaaccgtc catcttcggc
     4561 agcatcagat ccagcaggat gatatcaggg cccacttttg caaactgcgc caaggcttct
     4621 tcaccatcaa atgcagtgaa gacatcataa ccttctttgg tcagattaaa cttgattata
     4681 tctgaaattg gcttttcatc gtctaccact agtacctttt tcatcttcaa acctccttat
     4741 ttcttgaaac tctctgtccc gttcggaacg tctactcttt attatacaat tgcagccggt
     4801 tatgtgcaaa gcgttcctga attactatcc gtcagaatcc cggcggttgt accaaaaagg
     4861 acaagccatc aatccttttg ctgactgatg gcttatcccc ctattcgaca ttatggcatg
     4921 catatattga tttatcagct gtggatgacg accggaaccc cgataccgat catcccaaac
     4981 agttcagcca tgacccccgg tggcgtattg acacagccat tcgatccggc agtaagccaa
     5041 tattcaccgc cgtaagccgg ctgccaagaa gaatcgtgga taccgatgcc cgtccaatcg
     5101 actggcatcc agtattcgac aggcgattcg tagtcctttt ccgttctagg attgtagcct
     5161 ttcaaaacgg catcctcttc tttgttccag atgtagaaga ccccagtcgg agttggtgac
     5221 atcggatgac cggaaacgat atccgtttcg agcacaacgg cgccatcttt gtaataccac
     5281 atatgttggc ttgaaagatc cacttcgaca taggtagtgc cgatgtccgc gccatcagca
     5341 tgatagccgg taccgttata gtttggtgtt accgtaacat cttctcctgc caaaacatag
     5401 ttgaccagtt tatccgtttc ggctgcgaca gctagtgtcc atccgtaggt accggatgga
     5461 acctggaccg tttcgccgtc ggttgccaag aaatcgcgcg tacgggaata cgtgctgtat
     5521 ctgtcgctca gcccctgaag ataggctgca acgccttcac gattgaccga cacatcaccg
     5581 ttctcgtcta cgcccaacca ttcggtgatg gctgtttgcg gaacagtttc tgtctgtccg
     5641 gaaatggaat attggatgac cgtatcagtc aaatcattca atttctgcaa tgtttccgtc
     5701 aaaactggat cgtccgcggt caaggttggt tgctgatagg tctgcgcgag atcgatttct
     5761 gtttccccgg caatgatcgc ttccagcatt tcttgcttca acaattccag atcgactttg
     5821 tttccggtag tttcaggcga aatttcataa ccttccgttg ctttgacgac agtggcgttc
     5881 acgggcggag tacgtacttc gttctccaac ggcatagccg ccaaaatgtc ggccaaagct
     5941 gtctcatcga caactacgcc gctcagatcg gtttctgcct gcttttcttt gaaaagcagg
     6001 attggccaat tccaaacgtt ttgttctgcc ttcatcgttt ccaacttctc agtgttgtcg
     6061 atggacacac ccaaatcagc gggtgagaat gcatagatat tttctccatt ttcgacaatt
     6121 tggatctgtg tttgtgcgat tgctgctgtt gtctctgctt ttgcttgggc tacggtatca
     6181 ttcgatatat caatgccatc cagcatcgta ttcggtaaga atttatcctt ataatagcta
     6241 cctgcaccaa agtatccggc cacgaccaat aaaatcgctg tagcaccaat gatgatgggt
     6301 ttctttttca ctttcagacc accttctcgt ttcattacaa tcagattacg tttttgttac
     6361 aaataatcga agcttacttt ttattacttt accatgtttt cttgaaaaat caacctattt
     6421 taccttaatt ttttgataat aggatggatc tcaggcattc atcataatac caatacggaa
     6481 gaaatgcaaa cagacacact ccgctttcac tagctccgca atgttccagg cgagctgaaa
     6541 tgggcggcac ctcaatgttt tcctccggtg aacgcaggct tcgccggagt ttgactacct
     6601 tttgcgtgcg ttctccgata aagaccgctt cctcggagtt gcggtcgatt gttacattgt
     6661 ctgctccggc gggcggccgc ttgccggagt tagggccgtt ttctttcact cgctctcctc
     6721 cggcaaccaa gcagcacgca catcaaaaag agcctacctc aattcgaggc aagccctaaa
     6781 ttcctatgct tgattctgtt tctctgcttt gatttcgtcc aaaatatagc cgaaatcaaa
     6841 gtcttctttt ttgacgatca gcaagaggcc atccccgaga ggcacgaggg aggtttccaa
     6901 tgccggatgg tccagtacgg tatccatcag cttgttcaga cggcggtgga tcttgcggac
     6961 ccttttggga acgtcttttt catcatccaa gatggtgccg ccttggaaaa catcatcgat
     7021 gaccagcatg ccctcttttt tcagaagccg gatgcagtgg ggcaaaaatt cataatattt
     7081 ggatttcgcg ctgtccataa agatgaaatc atacggtcct tccaaagtag gcaggacttc
     7141 cgccgcatcg ccttccaaaa gcgtcacctt gtccgtgatg cccagttttt caaaattcgc
     7201 tttggcgtgg ttgatcatga cctcaaaacg atcaatggtc gtgacatgac cttccgctcc
     7261 gacgtgctgc gccatcagac tgcccgagaa gccgattgcc gctccgattt ccagtatctg
     7321 cttaggtttg atctgtccca gcagcatatt caggaaaacc gctgtttcat gcgggatgat
     7381 cggcacgcct ctttcattcg ctgtcttttc cagttctcct aattctccag ggaaacgttt
     7441 cagtctgtcg cgcataaaag cgaccagttc tttctttacg accggtctgt ccatcatttc
     7501 gttcaatgcc atactctttt ctccttatcg catattctat tattaatggc aacaaggctg
     7561 ggacggaatc ccagccttgc gcttattcat cgtctacaac cgctgtttgg acgggctctc
     7621 tcgtgacacg gatgccgcgg attcggccac tttcgacttg tgtagtcgtc agcaggtact
     7681 ctcctgtctg gatcgtcgca tgctcatcat ctttcggaaa atagccgagc tcctcgatca
     7741 tgtagccagc gatcgtgtcc acttcttcgg aagcgatcgc cgtcttgaac aactggttga
     7801 acttgtccaa cggcatgcgt ccattgacga cataatgatt gtcatcgatt tttttcgaca
     7861 ggcgataaat ttcatcatat tcatcttcaa tgtcccccac aatttcctcc agcaggtcct
     7921 cgagggtgat gattccctcc cccccgccgt attcatcctt aatgatggcc atatgcgtgt
     7981 gggtcttgcg gaattcgacc aggagatcat ctatatagat agtggaaggg acaaacaaag
     8041 gcgtgttgac caactcacgg aaagaaatcc gctcgaaacc gacttccttt gatgccttca
     8101 aaacattttt catatgcaga acgccgatga cattgtcctt atcttcctca tagaccggaa
     8161 tccgtgagta ttggctgttc agaaccagcg cgatattata ttccagatca tcatccaaat
     8221 ccatcatcaa agtgtcggta cgcggcacca tcaattcgcg ggcaagcttc gtatccaacg
     8281 aaaggactcc ctgcatcatc gtaaactcgg atggatcgat gactccttcg cctcggctct
     8341 gcgccagaat ggccttcatt tcatcacggg tgagcttttc ttcctcattc gtgaattcga
     8401 tcggcgtcaa cttcttcagg ataccggtcg agaaagacaa aagccaaacg aacggcttaa
     8461 aaagtgtctt gacgacaaca atgggacctg cagtatgacg cgcaatactt tcgggcatct
     8521 gcaaggcaat ctgtttaggg tacagctcac caaacacgag cgttatatag gaaaggatga
     8581 tggtgatgat gatcatcgct atcgttttac cacctgcgat gtttcccaac aacggttcga
     8641 tataaccgac aaaattggat gaagccgacg cactgctgaa gaatccggca aaagtgatgg
     8701 caacttggat agtggccaag aaatcatccg gattctccaa gagacgcaaa accttttgcg
     8761 atttcttatc gccgtctttg gcaagcttct cgattttgga gcggttgatg gaaacaaatg
     8821 ctaattcggc tgatgcaaag taagcattca gtaaagtcag ggttaggatc aaggctattt
     8881 ttgatagtaa cgactggcct tcggggtctg ggttcatatg atttttttct cctttttga
//
LOCUS       DAOMUJ010000020         8817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2029_length_8817_cov_2.405615, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000020 DAOMUJ010000000
VERSION     DAOMUJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_8817_cov_2.405615"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLT73_01115"
     CDS             complement(<1..973)
                     /locus_tag="PLT73_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM19013.1"
                     /translation="MGNKDGMNYAPKGKINRVVEPGAFNVGVTALDHGHINGMTNGLI
                     EAGATIKYVYDPDAEKVANYLESFPDVTVAESLEQILEDPEIQLVAAAAVPNLRSALG
                     NRVMRAGKDYFTDKTGFTTLEQLEETKKVVAETGKKYWVYFSERLHVEGAVFAGQLIE
                     DGRIGRVIQVTGFGPHRLDKEKRPDWFFKKEQYGGILADIGSHQIEQFLYYTGNTDAK
                     VLHSKVGNYNNPNTPELEDYGDATLIGDNGATFIFKVDWFTPDGLGTWGDGRTFITGT
                     EGTIEIRKYINVATEQTGDHLFLVTKDGEEHYSLTGEVGFPFFGEMIL"
     gene            complement(1046..1906)
                     /locus_tag="PLT73_01120"
     CDS             complement(1046..1906)
                     /locus_tag="PLT73_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRM19014.1"
                     /translation="MAKVKIGVQASTVKKSFEEVGPYETLKKLHEIGYNSIEISQVAT
                     TPENIAEIQKATADFGMEVASMSASLKPQVKDESLMTHYDKIVADCKAVGTDLLRIGM
                     LPIDAMASLDKVLEFCHDVNEVTQKLKEDGITLYYHNHHIEFRKYDGKFLLDIIREKA
                     PLLGFELDVHWVQRGGANPLEVIKDYKGKVELIHLKDYRIAEIPQDGFDALYSGDLAK
                     FMDYFTNTIQFAELGTGSLPLKEIIEESISSGARYLLVEQDDTYGVDPFESLKISREH
                     LLELGYGNLF"
     gene            complement(1899..2948)
                     /locus_tag="PLT73_01125"
     CDS             complement(1899..2948)
                     /locus_tag="PLT73_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM19015.1"
                     /translation="MLRAAIIGLGDVSPVHKYAIDVSDNGELVAVCDVNEALKTKYPD
                     VPFYTDVEAMLEKEDLDVVHICLPHYLHYPVTKLCVEKGVHVFQEKPLALDYEEGLKT
                     LALAEESSKKIGVCFQNRYNETFLELKRLLADENVGAVTGVKGLVAWHRPETYYTIKP
                     WRGQMEFAGGGSIINQAIHTLDLMQVLGGEIDRCKASLSNITDYDIEVEDTAVANFTF
                     KNGARGFYMSTNAYVENSSVELQVITEKSKFTIKDNCLYRTNSSGEKELHLQDTPIEG
                     TKFYYGPSHSALIQRFYNAIEADSDDYVTVRDALPAMLMIDAMKLSSKEKRTIEMEEI
                     RKVARRTRPTRIING"
     gene            complement(2968..4143)
                     /locus_tag="PLT73_01130"
     CDS             complement(2968..4143)
                     /locus_tag="PLT73_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM19016.1"
                     /translation="MTEKVKLGIIGYGAEGGMYAGFFKNNDERLNDNIELAAVCDNDP
                     AKKAKVAEDFPGLPFFDNYLDLLESGIVNAIVTTVPHYDHCVMGIAALERGIHLLGEK
                     PAGVYTKDVERLIETSKKHPETTFAIFFNQRTNPLYRRVKQLMDEKAIGDLQRATWII
                     TTWWRPQGYYNQSAWRATWGGEGGGVLVNQAPHQLDLLQWICGKPEKVYAKLQYGAGR
                     DIVVENEVNALLDFGNGATGSFITCTNDIVGTDRFEIFGTKGKIIVEDSKKLIVKQLT
                     APEAELSENMDMQDVMRLFMGQVNMEDYVKVTEEEFVTPQGLQHISVLNNFADHIVNG
                     EPLLANGEEGINGVTLANAMHLSSWLDKEVDYNVDGDLYLAELNKRIAEEGKFEQKK"
     gene            complement(4149..5012)
                     /locus_tag="PLT73_01135"
     CDS             complement(4149..5012)
                     /locus_tag="PLT73_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM19017.1"
                     /translation="MTRIVEKLLIGYHVKGQAYALDFHTHPQYEIFLFHGGNCRFLVG
                     NKIYYLQPGDLLMMDGMTVHRAYVIGDKDAYERSIVHFDADWITPLLNDLNIDYLLRF
                     FTENRNGLIRTFRKKNELLLENAIREMNHLQQLDQSYANEAKRKLALVQLLLMIDSST
                     DKIVEKGQAYVDEKTQIAEKVSEYIFKHYRDSFNIDDIASALNLSKSYLSHAFKEITG
                     NTIMAYAMGYRLSQACTALLMEPTKSIKMISGECGFESDAHFSRYFKQNTGTTPSNYR
                     KNNRISIQKGE"
     gene            complement(5387..7129)
                     /locus_tag="PLT73_01140"
     CDS             complement(5387..7129)
                     /locus_tag="PLT73_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM19018.1"
                     /translation="MPKLVDLKAKPYNLSDKQVAWVNETIAGMTDEEKVGQLFTNLFF
                     FGNDAFSGNDFTNQEIVEKFHIGGARYHGNSKEDIQDLLNDLQSMSKIPLLVAANCDS
                     GGNGACSEGTYIASGAQAEAAGDLQVSYHAGLVSAREEKALGVNVNFDPCVDILYNWR
                     NTIVNTRAYGTDAETVIKYTNAYLDGLTAENGENMEDGVIQCIKHFPGDGTEERDQHL
                     VLGVNELSPEEWRASFGKVYQNHIDRGVEMIMAGHIAVPALQKELNSALEDKDIMPAT
                     LAEELIQDALKTELGFNGLVITDATHMLGMTSAMRREDYVPKSIAAGCDMFLFFNNIE
                     EDFGFMLKGYQNGVITEERLTDALRRILGLKAKLHLPEKQANGTLLRTREDLNVIGCE
                     EHLQWRAEAADKGISLIKDTQNNLPINPVDHKRIRLYMLEGEKGGITDNGSKAQQLFV
                     DELTKRGYEVTVNERGSRVKGATLKYRDEVDFALEVADIAGYGAQNNYRIQWSTAMAN
                     EVPWFVWEVPHAFVSLNFTTHLHDATMVKTFVNAYHNNEETIKQVIDKLEGKSEFKGG
                     HNDLVWTDKWQAKL"
     gene            complement(7168..8016)
                     /locus_tag="PLT73_01145"
     CDS             complement(7168..8016)
                     /locus_tag="PLT73_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRM19019.1"
                     /translation="MSTHPTQFTKQKQFLVCVDSDGCAMDTMNVKHERFFGPLAADEY
                     GIKDRETFLADWNRINLFSSTRGINRFKALVLTLIEAQEKGEDIGDISALTDWANNAP
                     SLSNASLEAEIAKASSADLEKALVWSKKVNEGIETELAGEDKPFPGVLEGLTKIHGLT
                     DVAIVSSANSEALNSEWNRHNLMPQVDVVYGQEVGSKADAIADLLTKGYAADEILMVG
                     DAPGDEQAAAVNGVFYYPILFGKEEFSWERLSNEAIGKFLNKEYAGEYQAKVLGEFHA
                     LLAQFD"
     gene            complement(8112..>8817)
                     /locus_tag="PLT73_01150"
     CDS             complement(8112..>8817)
                     /locus_tag="PLT73_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM19020.1"
                     /translation="AQGYADEIVAEGGIAKAFKANVLELPSLQAAKEAVVAELGEVDI
                     LVNGAGGNNPRATTDNEYHETDLPEGTKTFFDLEQSGIEFVFNLNFLGTVLPTQVFAK
                     DMVGRPGANIINISSMNGFTPLTKIVAYSGAKAAISNLTEWLAVHFAHVGIRVNAIAP
                     GFLVSKQNQALLFNEDGTPTPRTGKILNGTPMGRFGEPHELIGGLLFLADDKAASFVN
                     GVVLPIDGGFNAYSGV"
BASE COUNT         2103 a         2190 c         1871 g         2653 t
ORIGIN      
        1 ccaagatcat ttcgccgaag aacgggaacc cgacttcgcc tgtcaaggaa tagtgctcct
       61 cgccatcctt cgtcaccaag aagaggtgat cgccggtctg ctccgtcgct acgttgatgt
      121 atttgcggat ttcgatcgtt ccttctgttc cggtgatgaa cgtgcggcca tcgccccacg
      181 ttccgagtcc gtccggtgtg aaccagtcca ctttgaagat gaaggttgcg ccattgtccc
      241 cgatcagcgt agcgtcaccg tagtcctcca attctggtgt attcggattg ttgtagtttc
      301 cgactttgct gtgcagcact ttggcatcgg tatttcccgt atagtaaagg aattgctcaa
      361 tctgatggct gccgatgtcg gccaaaatgc cgccgtattg ttctttcttg aagaaccaat
      421 ccggacgttt ctctttatcc aatcgatgcg gcccgaaacc ggtcacttgg atgacacggc
      481 cgattctgcc gtcttcgatc aactggccgg caaatacagc accttccaca tgcagtcttt
      541 cgctgaaata gacccaatat tttttaccgg tctctgcaac tacttttttg gtctcttcga
      601 gttgttccag tgtcgtgaag ccggtcttgt ccgtgaaata gtctttccct gcccgcatga
      661 ctcgatttcc caacgcactg cgcaagttag gcacagcagc agcggcaacc aactggattt
      721 ccggatcttc caaaatttgt tccaaagact ccgcaacggt aacatccggg aatgattcca
      781 gataattcgc taccttttcc gcatccggat cgtagacata tttgattgta gcgccggctt
      841 caatcaaccc gttcgtcatg ccgttgatat gtccgtggtc cagtgccgta acgcccacgt
      901 tgaacgctcc cggttccact actctgttta ttttcccttt cggagcataa ttcattccat
      961 ctttatttcc caatcgtatg acctccttca ttattatagg aagaagtccg aagagaatcc
     1021 tctccgaact tcttcatcgc ttttatcaga acaggtttcc gtagcccaat tccaataggt
     1081 gctcacggga tattttcaag ctttcgaacg gatccactcc atacgtgtca tcctgctcca
     1141 ctaataggta gcgagcacct gatgagatgc tttcctcgat gatttctttc aaaggtaggc
     1201 tgcctgttcc caattcagcg aattggattg tatttgtgaa gtaatccata aatttggcaa
     1261 gatccccact atataaagca tcgaagccat cttgtgggat ttcagcaata cggtagtcct
     1321 tcaaatggat caattccact ttgcctttgt agtctttgat gacttccagt gggtttgcac
     1381 caccgcgttg cacccagtga acatccaact caaagcccaa caatggcgct ttttcgcgaa
     1441 tgatgtccag caggaattta ccgtcatatt tgcggaattc gatgtgatgg ttatgataat
     1501 acaaggtgat tccgtcttct ttcagctttt gcgtcacttc attgacatca tggcagaatt
     1561 ccaacacttt atccagactg gccatcgcgt cgatcggtaa catgccgata cgtaacaggt
     1621 ccgttcccac ggctttgcag tccgctacga ttttatcata gtgtgtcatc aaagattcgt
     1681 cttttacttg cggtttcaag gaggcactca tggaagctac ttccatcccg aaatcagcgg
     1741 tcgctttttg aatttcagca atgttctcag gcgttgtcgc cacttgcgaa atttcaatcg
     1801 aattgtagcc gatttcgtgc agtttcttca gtgtttcata aggtcctact tcttcaaaac
     1861 tctttttgac cgttgacgct tgtacaccta tcttaacttt agccatttat gattcgcgtt
     1921 ggccgcgtgc gcctggctac cttcctgatt tcctccattt cgattgtacg tttttctttt
     1981 gatgacaatt tcattgcatc gatcatcagc atcgccggta aagcatcacg gaccgtcaca
     2041 tagtcatccg agtctgcttc gattgcattg tagaaacgct ggatcagtgc agaatgactt
     2101 ggtccgtaat aaaattttgt tccttcaatc ggtgtgtcct gcagatgcag ttctttttca
     2161 cccgaactat tcgtgcggta aagacagtta tccttgatcg tgaatttcga cttttcggtg
     2221 atgacctgca attctacgct ggaattctcg acataggcat tggtcgacat gtaaaatccg
     2281 cgcgcgccgt tcttgaaggt gaaattcgcg acggcagtat cctcgacctc gatgtcatag
     2341 tccgtgatgt tggacagact ggctttgcat cggtcgattt cgccgcccaa aacctgcatc
     2401 agatcaagcg tatggatggc ttgattgatg atggatccac cgccggcaaa ttccatctga
     2461 ccgcgccatg gtttgatggt gtagtaggtt tcaggccgat gccatgccac caagcctttt
     2521 acccccgtca cagctccgac gttttcgtcc gcgagtaatc ttttgagttc caaaaacgtt
     2581 tcgttgtaac gattttggaa acacacgcca attttcttgc tgctttcctc tgccaaagcg
     2641 agcgtcttca agccttcctc ataatccaaa gccaaaggtt tttcctggaa aacgtgcacg
     2701 cctttttcaa cacagagttt cgtgaccgga taatgcagat agtgcggcaa gcagatatgt
     2761 acaacatcca aatcctcttt ttccaacatc gcttctacgt ctgtataaaa aggtacatca
     2821 ggatatttgg tcttcaaggc ttcatttacg tcgcaaaccg caaccaattc accgttgtca
     2881 ctgacgtcga tagcatattt gtggacaggc gatacatctc ctaatccgat tattgccgct
     2941 cttaacatcc ctgatcacgt cactttctta ttttttttgt tcgaattttc cttcttcagc
     3001 gatgcgtttg ttcaattcag ccaaatacag gtcgccatcc acattgtaat ccacttcttt
     3061 atccaaccaa gaggacaagt gcatcgcatt cgccaaggtc accccgttga tgccttcttc
     3121 accattggcc aacaaaggct ccccgtttac gatatgatct gcaaaattat tcaacacaga
     3181 aatgtgttgc agtccttgag gcgtcacaaa ttcttcttca gtgaccttta cgtagtcctc
     3241 catgttgacc tgtcccatga agagtctcat gacatcctgc atgtccatat tttcggacag
     3301 ttcagcttcc ggagcagtca attgcttcac aatcagtttt ttcgaatctt caacgatgat
     3361 ttttcctttc gtcccaaaga tttcgaagcg gtccgtgccg acgatatcgt ttgtgcaagt
     3421 gatgaacgaa ccggtagcgc cgttaccgaa gtccaagagt gcattcactt cattttcaac
     3481 aacgatgtca cggcctgcgc catattgcag tttcgcgtac actttttcag gctttccgca
     3541 gatccattgc agcaggtcca attggtgagg agcttggttc acaagaacgc cgccgccttc
     3601 tccgccccag gttgcacgcc atgcgctttg attgtagtag ccttgcggtc tccaccaagt
     3661 tgtgatgatc caagtagcac gctgcaagtc gccgatcgct ttttcatcca tcaattgttt
     3721 tactctacgg taaagtgggt ttgtacgttg attgaagaaa atcgcaaaag tagtttctgg
     3781 gtgttttttc gatgtttcga tcaaacgctc cacatctttc gtataaacac ccgccggctt
     3841 ctctcccaat aagtggatgc cacgttcaag tgcagcgatc cccatcacgc agtggtcata
     3901 gtgcggaaca gtcgttacga ttgcgtttac aataccggat tccaaaaggt ccaggtaatt
     3961 gtcgaagaaa ggcagtccag ggaagtcttc agcgactttc gcttttttag caggatcgtt
     4021 gtcgcagacc gctgccaact cgatattatc gttcaggcgc tcatcgttgt tcttgaagaa
     4081 ccctgcgtac ataccgcctt ctgcaccata accaataatt cctaatttca ctttttctgt
     4141 cattttcatc attctccttt ttgtatgctg attctattat ttttcctgta attactgggt
     4201 gtagtgccgg tgttctgttt aaaatatctg ctgaaatggg catcgctttc aaacccacat
     4261 tcgccggaaa tcattttgat cgatttggtt ggttccatca atagtgccgt gcatgcttgg
     4321 gataaccggt atcccatcgc ataagccatg atcgtatttc ctgtgatttc tttgaaagca
     4381 tgcgacaaat aggatttgct caaattaagg gcagaggcga tatcatcgat gttgaacgag
     4441 tcccggtaat gcttaaaaat atattccgaa accttttcgg ctatctgtgt cttttcatcg
     4501 acataagcct gtcctttttc tacgatctta tccgttgaag aatctatcat cagaagcaac
     4561 tggactaaag ccagtttgcg tttcgcttca tttgcatagg attggtccaa ctgctgcaaa
     4621 tgattcattt cccggatagc attttctaaa aggagttcat tcttctttcg gaaagtccga
     4681 atgaggccgt tacgattttc agtaaagaaa cgcaacaaat agtcaatgtt cagatcattc
     4741 agtaaagggg tgatccagtc ggcatcgaaa tggacgatgc tgcgttcata ggcatccttg
     4801 tctccaatta cgtaggcacg gtgaaccgtc atcccatcca tcatcagcaa gtcgccaggc
     4861 tgaagataat agattttatt accgaccagg aaccgacaat ttccgccatg gaacaagaat
     4921 atttcatatt gcggatgtgt atggaagtca agggcatatg cttgtccttt gacgtggtat
     4981 ccgatcaaca atttttcaac gatacgtgtc acctccctct ctttgacact tcaaagtata
     5041 aaataacagc gtttacaatt caatgtcctg gaatgcttag attatttaaa tcattgacca
     5101 agagtgcttt ttgttgttca aaaaaaacaa agtcgagaaa aattttctcg actttacttg
     5161 gtacaacagt gttttgggtg atttgtgaac aaaatggtac attgcttcaa tggtcacgat
     5221 tcagatgctc aaactctgag ctcaggattc atcacacact gattacagtt gaactctgaa
     5281 tcccagaaac ctgtgcctaa gctgacaacc acacacggac aaaaagcgtc cgctttgtgt
     5341 ttgtcctctt agtgctcggt ttctacccgg gattcatcgt atccttttat aatttggctt
     5401 gccatttgtc ggtccaaacg aggtcgttgt ggccgccttt gaattcggat ttgccttcca
     5461 gtttatcaat gacttgtttg atggtttctt cattgttgtg gtaagcattg acgaatgtct
     5521 tcaccatagt cgcatcatgc aggtgtgtcg tgaagttcaa tgatacgaag gcatgcggaa
     5581 cttcccaaac aaaccaagga acttcatttg ccatggcagt gctccattgg atacggtagt
     5641 tattttgtgc tccgtagcca gcgatgtcag caacttccaa agcgaaatca acttcgtcac
     5701 ggtatttcaa agttgcgcct ttaacacgag agccgcgttc atttacagtc acttcatagc
     5761 cacgtttcgt cagttcatcc acgaacagtt gttgcgcttt cgaaccgtta tccgtgatgc
     5821 cgcctttttc gccttccagc atgtaaagac ggatgcgttt gtggtcgact ggattgatcg
     5881 gcaagttgtt ttgggtatct ttgatcaaag agatcccttt atcagcagct tccgctctcc
     5941 attgcagatg ttcttcacag ccgatgacgt tcaaatcttc acgcgtgcgc aacagagtac
     6001 cgtttgcttg tttttcaggc aagtgcaatt tagctttcaa gcccaagata cgacgcaaag
     6061 catcagtcaa acgttcttcc gtgatgacgc cgttttggta acctttcagc atgaagccga
     6121 agtcctcttc gatgttgttg aagaacagga acatatcgca gccggcagcg atcgatttcg
     6181 gaacgtaatc ttcacggcgc atagcggacg tcatacccaa catgtgcgtt gcatccgtga
     6241 tgaccaagcc gttgaagcct agttcagtct tcaatgcatc ttggatcaat tcttcagcca
     6301 atgttgcagg catgatatct ttgtcttcca aagcagagtt caattctttt tgcaaagcag
     6361 ggacagcaat gtgtcctgcc atgatcattt caacgccgcg atcgatatga ttttggtaca
     6421 ctttaccgaa tgaagcgcgc cattcttcag gacttaattc attcactccc aaaaccaaat
     6481 gttggtcgcg ttcttcggtt ccatcgccag ggaagtgttt gatgcactgg atcacgccat
     6541 cttccatgtt ttcgccgttc tccgcagtca atccgtccaa ataagcattt gtgtacttga
     6601 tgacagtttc agcatccgtg ccgtaagcac gcgtattgac gattgtgttt ctccagttgt
     6661 aaaggatgtc cacacatggg tcaaagttga cgttgacgcc taaagctttt tcttcacgcg
     6721 cggaaaccaa tccggcatgg taagaaacct gaaggtcgcc tgcagcttca gcctgggcac
     6781 cggaagcgat gtatgtgcct tcactgcaag cgccgtttcc gcctgaatcg cagttcgcag
     6841 ccaccaacag aggaatcttg gacatggatt gcaggtcgtt caatagatct tggatatctt
     6901 ctttcgaatt gccgtgatag cgtgcgccac cgatgtggaa tttttcaacg atttcctggt
     6961 tggtgaagtc atttcctgag aaggcatcat ttccaaagaa gaaaaggttc gtgaacaatt
     7021 gccctacttt ttcctcatcg gtcatgccgg cgatcgtttc gtttacccaa gcgacctgtt
     7081 tgtcgctcag attatatggt tttgctttta aatcaactaa ttttggcact gtttttcctc
     7141 ctgctaacgt aatttttgga atatagatta atcgaattga gccaataaag catggaattc
     7201 tccaagcact tttgcttggt attctcccgc atattctttg ttcaggaatt ttccgattgc
     7261 ttcattgctc agacgttccc aagagaactc ttctttgccg aagaggatcg gataatagaa
     7321 tacgccattt accgctgccg cttgttcatc gccaggtgcg tcgccgacca tcaaaatttc
     7381 gtccgctgca taacctttcg tcaacaaatc agcgattgca tccgctttgc tgccgacttc
     7441 ttgtccgtaa actacgtcaa cttgcggcat cagattgtgt ctgttccatt cgctgttcaa
     7501 agcttcgctg ttggcagaac ttacgattgc gacatccgtc aagccatgga tttttgtcag
     7561 tccttcgaga acgccaggga acggtttgtc ttctccagcc aattcggttt cgatgccttc
     7621 attgactttc ttgctccata ccaaagcttt ttccagatcg gcagaagacg cttttgcgat
     7681 ttccgcttca agggatgcat tcgataacga tggcgcattg tttgcccaat cggtcagtgc
     7741 cgaaatatca ccgatatctt cgcctttttc ctgtgcttct atcaacgtca aaaccaatgc
     7801 tttgaagcga ttgatgccgc gtgtgcttga aaacaggttg atgcggttcc agtcagccaa
     7861 gaaagtttcg cgatctttga tgccatactc atccgctgcc aaaggaccga agaaacgttc
     7921 atgcttcacg ttcatcgtat ccatagcgca accgtcggaa tcgacgcaga ctaagaattg
     7981 tttttgtttc gtaaattgtg ttgggtgtgt agacatattt tttctctcct aacttgatat
     8041 caaaaaaagg acagatagag aagaactgtg caacacattt cgttctatct gccctattca
     8101 attacctaaa cttatacgcc tgaatatgcg ttgaagccgc catcgatcgg caatactacg
     8161 ccgttcacaa agcttgcagc tttatcgtca gccaagaaca gcaagccgcc gatcaattca
     8221 tgcggttcgc cgaaacggcc cattggtgtg ccgttcagga ttttacctgt acgaggagtt
     8281 ggtgtgccat cttcattgaa caatagcgct tgattctgtt ttgaaaccaa gaaaccagga
     8341 gcgatcgcat tgacacggat acctacgtga gcaaagtgaa cagccaacca ttcagtcaag
     8401 ttggagatag cagcttttgc gcctgagtaa gcaacgattt ttgtcaatgg tgtgaaaccg
     8461 ttcatgcttg agatgttgat gatgttagcg cctggacggc caaccatgtc tttagcaaaa
     8521 acttgtgtag gcaatactgt acccaagaag ttcaagttga agacgaactc gataccgctt
     8581 tgttccaagt cgaagaatgt ttttgttcct tcaggcaaat cagtttcatg gtactcgttg
     8641 tcggttgttg cgcgcgggtt gttgccgcct gctccgttga ccaagatatc cacttcacct
     8701 aattcggcta cgacagcttc tttagctgct tgcagacttg gcaattccaa tacgttggct
     8761 ttgaacgctt tagcgatgcc gccttcagca acaatttcat cagcatagcc ttgtgcg
//
LOCUS       DAOMUJ010000021         8599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2107_length_8599_cov_2.305712, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000021 DAOMUJ010000000
VERSION     DAOMUJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_8599_cov_2.305712"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1193
                     /gene="atpB"
                     /locus_tag="PLT73_01155"
     CDS             477..1193
                     /gene="atpB"
                     /locus_tag="PLT73_01155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRM19021.1"
                     /translation="MEAESKVIHFMGLGFSLNIIISVLATCIIVFLFCFIATRKLAVR
                     PTSKSQLAIEYLVDFIRNMISSSLHWKVGQQFHLLGFTLFLFVWVANMLGLVFILNID
                     GHSYWKSPTASPIVTLGLALMVILLTHYFGIKENGFKEYFMNSYIRPVAPLMPIKLLE
                     EFTNTLTLALRLYGNIYAGEILLGLIASLANSYGLLTWVVGIPLQMVWQGFSIFIGSI
                     QAFVFTTLTMVYLAHKIEAE"
     gene            1256..1468
                     /gene="atpE"
                     /locus_tag="PLT73_01160"
     CDS             1256..1468
                     /gene="atpE"
                     /locus_tag="PLT73_01160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRM19022.1"
                     /translation="MNFIGAAIAVAGAAIGASLGNGKVISTTIEAIARQPELQSRLQT
                     LMFIGVGLIEAVPIMAVVIAFLLIFQ"
     gene            1508..2011
                     /gene="atpF"
                     /locus_tag="PLT73_01165"
     CDS             1508..2011
                     /gene="atpF"
                     /locus_tag="PLT73_01165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRM19023.1"
                     /translation="MTANLVLGEITALGDTIVTLLSFLILMALIKKFAWQPLMKILTD
                     REEMIHANIDKAAAARENAEKKHAEVDAQLIEVRTTASDILNRAQAEGNEIQKNIIKE
                     AKEDALRIKQVTQKELELERKQALEGLRAEIGVISVEIAEKILGKEIKQADQQTLIEA
                     FIEGLDD"
     gene            2004..2531
                     /locus_tag="PLT73_01170"
     CDS             2004..2531
                     /locus_tag="PLT73_01170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRM19024.1"
                     /translation="MIDMVKDGKAITTVEAYVQAYYNRLTDSKTADRLYLDLSQLKEA
                     FNHADSDAGEQPNLDLAVKYLAPDTLRFLEGLQDNLDNEGILASIKAFNELYENKKVL
                     VNVKLTSAVPLTAEQKMRIMNKMEKRIGEATFFVKEVVDPTVIGGVRLESENHYFDNT
                     IATKLKEMKRHILTD"
     gene            2554..4065
                     /gene="atpA"
                     /locus_tag="PLT73_01175"
     CDS             2554..4065
                     /gene="atpA"
                     /locus_tag="PLT73_01175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM19025.1"
                     /translation="MATENEDLSSLIKNRINTFQSVSEFEEIGKVTYIGDGIARAKGL
                     ENVMSGELLEFSNGSIGMAQNLEMTDVGIIIFGTYENIHEGDIVKRTGKIMEVPVGDA
                     LIGRVVDALGSPVDGLGSIETTGTRPVENAAPGVMDRQSVRQPLQTGLKALDALVPIG
                     RGQRELIIGDRKTGKTSIAIDAILNQRGKDVICIYVAIGQKESTVRNMTETLKKHHAM
                     DYTIVVTASASQSAPMLYIAPYAATAMAEEFMHNGKHVLIVYDDLSKQAAAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKLNDELGGGSITSLPIVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFFSGIRPALSAGLSVSRVGGSAQIKAMKKVSGTLRIDLASYREL
                     EAFTQFGSDLDVSTQKRLGRGRRTVEILKQDLHKTLDVEKQVLILYALTHGFIDSIPV
                     QDIERYESELFKFVKMSYPNLFSIIKETKDLPPTEDLDEALKAFAGIFIPHVE"
     gene            4103..5023
                     /locus_tag="PLT73_01180"
     CDS             4103..5023
                     /locus_tag="PLT73_01180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRM19026.1"
                     /translation="MAESLIDIRKRIASTKKTSQITSAMQMVSASKLIRAEQKVRGYQ
                     LYAAKIREIVTHIASTQLSLLEEHGHVSASDQVLVDFHDMLIERPVRKTGYLIISSDK
                     GLAGGYNSSILKATKDMLAVDHASNDEFVVLSVGGAVADDLRKSGIEVAFEIRDLNDQ
                     PSFTEVSDIVVKAIQLYRDGVFDELYVCYKHHVNALISEYRAEKMLPLTDLDSQEAVG
                     YEVDYLFEPNKEEILEILLPQYAESLIYGAIIDAKSSEHASRMTAMKSATDNATNMIN
                     DMTIQYNRVRQTAITQEITEIVGGASALED"
     gene            5042..6475
                     /gene="atpD"
                     /locus_tag="PLT73_01185"
     CDS             5042..6475
                     /gene="atpD"
                     /locus_tag="PLT73_01185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM19027.1"
                     /translation="MKKGHIVQVIGPVVDVGFPLEEGVPDIRNALIVHKAAAAGSNEE
                     GETIVLEVSLELGNGVVRAIAMESTDGLQRGLTVVDTGAPIKVPVGLGTLGRVFNVLG
                     EAIDLKGAVPDDFRRESIHRKAPTFDQLSSNFEILETGIKVIDLLAPYLKGGKIGLFG
                     GAGVGKTVLIQELIHNVAEQHGGISVFTGVGERTREGNDLYHEMQESGVGKKTAMVFG
                     QMNEPPGARMRVALSGLTMAEHFRDEEKQDVLLFIDNIYRFTQAGSEVSALLGRMPSA
                     VGYQPTLATEMGQLQERISSTKDGSITSIQAIYVPADDYTDPAPATTFAHLDATTNLE
                     RKLTEQGIYPAVDPLASSSSALSPEIVGDEHYEVATKVQRILQRYRELQDIIAILGMD
                     ELSDSEKITVNRARRIQFFLSQNFHVAEAFTGVPGSYVPVAETVRGFKGIVEGKYDGV
                     PEEAFRNVGTIEEVLKKAKELGYKGGE"
     gene            6477..6902
                     /locus_tag="PLT73_01190"
     CDS             6477..6902
                     /locus_tag="PLT73_01190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRM19028.1"
                     /translation="MAYLQVNVVTPDGIAFQHRAKAVTATTTDGGITIMPNHTPIIVP
                     LAIADLIVTRVTDELAQNHIAVNGGIMEVRDNVVNIIANSAERARDIDIDRAEVAKER
                     AEKRMAEARDFKNEKEFQRAKISLSKAINRIGVSKNRSN"
     gene            7159..7413
                     /locus_tag="PLT73_01195"
     CDS             7159..7413
                     /locus_tag="PLT73_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HRM19029.1"
                     /translation="MGGVRHLDLTLFNGIIVLLSHMIFIFISFWSLKAIRLEKWIRKG
                     HIREARVLYFFLSIALGFTVSTFLLELVTTSRNISYLLFS"
     gene            7532..>8599
                     /gene="murA"
                     /locus_tag="PLT73_01200"
     CDS             7532..>8599
                     /gene="murA"
                     /locus_tag="PLT73_01200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003644659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM19030.1"
                     /translation="MEKIIVRGGNRLEGTVKVEGAKNAVLPILAATLLASEGQSVLTN
                     VPILSDVFTMNNVLKHLNVGVEFQEKNNTILADASNDINFEAPFAYVSKMRASIVVMG
                     PLLARLGHARIALPGGCAIGTRPIDLHLKGFEAMGADIKIENGFVEAKVDRLVGARIY
                     LDFPSVGATQNIMMGATLAEGTTIIENVAREPEIVDLANFLNRMGAKVVGAGTETIRI
                     EGVSSMKATEHSIIPDRIEAGTFMIAAAVTNGNVFIEDAIADHNKPLISKLKEMHVAF
                     QEEENGLRVIGPRSLKATDVKTMPHPGVPTDMQAQMTIAQLVSQGTSTMTETVFENRF
                     MHLEEMRRMNADFKVEGQTVLI"
BASE COUNT         2480 a         1738 c         2092 g         2289 t
ORIGIN      
        1 aagagggttt ctggctcatt tttattgttc aagttaagtt tgttatactt taattggata
       61 aggctacagt atcgaaggtt ttcaaacata gcgttaggag caataaagtt gcttttattt
      121 ccactgtaaa atcactttag cgttaatttt cggattttca gaatcctata attatattta
      181 cacttcgagt atctttggtt gtgaaggcgg tatcttttca tcattttcat aaaaaaaccg
      241 aaaatatact tataatatgt agatttgctt tgaatttcga taaacttggt gttatgatta
      301 gcgatgttga acagatgtgc aaagaaactt atgaaatagc cacttattgt gactagagta
      361 tgggaggtcc agctgaatta gttgtctact tttacgaatg tcttttaaaa taatgtagcg
      421 tggactaggt ttggcatagc tgtttttttt atttgtattg aaaagaggtg aaaatattgg
      481 aagctgaatc taaagtcatc cattttatgg gattgggttt cagtttgaat atcatcataa
      541 gtgtattggc gacatgcatc attgtttttt tattttgttt tattgctacc cggaaactgg
      601 cggtgaggcc aacatccaaa agccaattgg ctattgaata tttggttgac tttatccgga
      661 acatgatttc cagctcgctt cattggaaag ttgggcaaca attccacttg ttgggattca
      721 ctttgttcct atttgtatgg gttgccaata tgttaggact tgtgttcatt ttgaacatcg
      781 atggccattc ctattggaag agtccgacag ccagtccgat cgtcacatta ggcttagcgt
      841 taatggtgat tttgctgacg cattatttcg gtattaaaga aaatgggttc aaggaatatt
      901 tcatgaacag ttatattcgt ccggtcgcac cgctcatgcc gatcaaactt ttggaggaat
      961 tcacgaatac attgacattg gcgttgcgtc tttacggaaa tatttatgct ggtgaaattt
     1021 tgttggggtt gattgcttca ttagcgaatt catatggctt attgacttgg gtggttggga
     1081 ttcctctcca aatggtttgg caaggatttt ccatctttat cggatccatc caagctttcg
     1141 tcttcactac gttgactatg gtttacctcg cccataagat cgaagcggaa taaaactgct
     1201 gatccatgag cttaataagt atgtattaat atatgagaaa aaggagatta ttattatgaa
     1261 ctttatagga gctgctattg cggttgccgg cgcggccatc ggagcatcat taggaaatgg
     1321 aaaggttatt tctactacaa tcgaagcgat cgcaagacaa ccagaattgc aaagcagatt
     1381 acagactttg atgttcatcg gtgtcggttt gatcgaagct gttcccatca tggcggtagt
     1441 tatagctttc ttactgattt tccaataata gggaccagca agaccgataa ctacaggagg
     1501 gatgagaatg acagccaatc tcgtattggg agagattacc gcattaggtg atacgattgt
     1561 tacgctactg tcatttctga tattgatggc tcttatcaaa aaattcgcat ggcagccttt
     1621 gatgaagatc ttaacggatc gtgaagaaat gatacatgca aatattgata aagcggcagc
     1681 tgcgcgtgag aatgccgaga agaaacatgc ggaagtcgat gcgcaattga tagaagtcag
     1741 aactacagcg agcgatattt tgaacagggc tcaagcggaa ggcaatgaaa tacaaaagaa
     1801 catcattaaa gaagcaaaag aagatgcatt acggatcaaa caagtgaccc aaaaagagct
     1861 ggaactcgaa agaaaacagg ctcttgaagg tttgcgtgcc gaaatcggag tcatatcagt
     1921 agaaatcgct gaaaagatcc tcggcaagga aatcaagcaa gcagaccaac aaacattgat
     1981 tgaagctttc attgaagggc tggatgattg atatggttaa ggacggcaaa gcaatcacaa
     2041 ctgtagaagc ctatgtacaa gcctactata accgcctgac tgactcgaag actgcggaca
     2101 gactctatct tgatctttcg cagttaaaag aagccttcaa tcatgcagat tcagatgcag
     2161 gagagcagcc aaatcttgat ttggctgtga agtatcttgc tcctgatacg cttcggtttc
     2221 tagagggact ccaagataat cttgataacg aaggtatctt ggcttccatc aaagcgttca
     2281 atgagctgta tgaaaataaa aaagtactcg tgaatgtgaa gttgacttct gcggttcctt
     2341 taacagcgga acaaaaaatg cgcatcatga acaaaatgga aaaaagaatc ggggaagcga
     2401 ccttctttgt gaaagaggtg gtcgatccca ctgtgattgg cggcgtccgg ttggagtcgg
     2461 aaaaccatta tttcgacaat accatcgcta cgaagttgaa agaaatgaag agacatatcc
     2521 taacagatta atagggttga agaggtgaat aatatggcta ctgaaaacga agatttgagt
     2581 tcattgatca aaaataggat caacactttt caatctgtat cagagtttga agaaattgga
     2641 aaagttacct acatcggtga tggaatcgcc cgtgcgaaag gtttggagaa cgtaatgtcc
     2701 ggtgaattgc tggaattctc caacggttcc atcggtatgg cccaaaacct ggagatgacc
     2761 gatgtcggta tcatcatttt tggaacttat gaaaatatcc atgagggcga tatcgtcaaa
     2821 cgtacaggca aaatcatgga agtaccggtc ggtgatgctt tgatcgggcg cgttgtggat
     2881 gcattaggca gcccggtgga tggcttaggc agcatcgaaa ccaccggcac aagacccgtt
     2941 gaaaatgcag caccaggcgt tatggatcgt caatccgtca gacaacctct tcagaccggt
     3001 ctaaaagcat tggatgcttt ggttccgatc ggtcgaggcc aacgcgagct gatcatcggt
     3061 gaccgcaaaa ccgggaaaac cagtattgca atcgatgcaa ttctgaatca acgcggaaaa
     3121 gatgttatct gtatctatgt cgccatcggg caaaaggaat cgacagtccg caatatgacg
     3181 gaaacgttga agaagcatca tgcaatggac tatacgattg tcgtgaccgc aagtgcttca
     3241 caatctgcgc cgatgctcta tatcgcacct tacgccgcta ccgcaatggc ggaagagttc
     3301 atgcacaacg gcaagcacgt tcttatcgtt tatgatgatc tttccaaaca ggcggctgct
     3361 taccgtgaaa tgtccttgct tttgcgtcgt ccgccaggcc gcgaggcgta tccaggggat
     3421 gtcttctact tgcattcccg tttgttggaa cgttcagcaa aattgaacga tgaattgggc
     3481 ggtggctcca taacctcctt gccgattgtt gaaacacaag ccggggatat ctccgcttat
     3541 attccgacga acgtcatctc catcacggat ggtcagattt tcttggaaag cgacctgttc
     3601 ttctcaggaa tccgtccggc tctatctgcg ggtctctccg tttcccgtgt cggaggatct
     3661 gctcaaatca aagccatgaa aaaagtatcc ggtacattac gtatcgattt agccagctat
     3721 cgcgagctgg aagcattcac ccagttcggt tcggacttgg atgtttccac gcaaaaacgt
     3781 cttggccgcg gccgcagaac cgtggaaata ctgaagcagg acttgcacaa gaccttggat
     3841 gtcgaaaaac aagttctcat tctctatgca ttgacccacg gatttattga ttcgattccg
     3901 gtgcaggata tcgaacgata cgagtctgaa ctgttcaaat tcgtcaaaat gtcttatccg
     3961 aatctgttct ccatcatcaa ggagacaaaa gacttaccgc ctacggaaga cttggatgaa
     4021 gcgctgaaag cattcgcggg aatcttcatt ccgcacgtcg agtagaaggg tcagcatgaa
     4081 cagataatat tgaggtgata atatggcaga gtctttaata gacatccgga aacgcattgc
     4141 ttccacaaag aagacaagtc aaataacaag cgccatgcaa atggtatctg cctccaaact
     4201 gatccgcgcc gaacaaaagg tgcggggata tcagctttat gcagcaaaaa tacgcgaaat
     4261 cgtaacacat attgccagca cgcaattatc tttgcttgaa gagcatggtc atgtgtcggc
     4321 gagcgatcaa gttcttgtcg attttcacga tatgctgatc gaacggccgg tccgcaaaac
     4381 gggctatctg atcatttcct cagataaagg cttagccgga ggatacaaca gttcgatcct
     4441 gaaagcgaca aaagatatgc ttgcggtcga ccacgcttcg aacgatgagt ttgttgtgct
     4501 gtcggtcggt ggagctgtgg ctgatgatct cagaaaaagt gggatcgaag tcgcattcga
     4561 aatcagggac ttgaatgacc aaccaagttt cacggaagtc agcgatatcg tagttaaggc
     4621 aatccaactt tatcgtgatg gggtttttga tgagttgtac gtttgttaca aacatcatgt
     4681 caacgctctg atttcagaat atcgagctga aaaaatgctg ccgttgacgg acttggattc
     4741 ccaagaagcg gttggttacg aagtcgatta tctatttgaa ccgaacaaag aagaaatatt
     4801 agaaatatta ctgccgcaat atgcggaaag tttgatttac ggtgccatta tcgatgctaa
     4861 atcatccgag catgcttccc gcatgacggc catgaagagt gctactgata atgcgaccaa
     4921 tatgatcaat gatatgacga ttcagtacaa ccgagtgaga cagactgcaa tcacgcaaga
     4981 aatcaccgaa atcgtcggtg gagcttctgc tctggaagat tagaaaatgg gaggaaaagg
     5041 catgaaaaaa ggtcatattg tacaagtaat cgggccggta gtcgacgttg gcttcccatt
     5101 ggaagaaggt gtgccggaca tccgtaatgc gcttattgtg cataaggcag ccgcagctgg
     5161 aagtaatgaa gaaggggaaa ccatcgtttt ggaggtttca cttgaattgg gaaatggtgt
     5221 ggttcgcgca atcgcgatgg aatcgacaga tggcctgcaa aggggcttga ctgttgttga
     5281 taccggtgcg cccatcaaag taccagttgg tttaggcacc cttggccgtg tgttcaatgt
     5341 tttgggtgaa gccatcgatt tgaagggagc tgttcctgat gatttccgtc gtgagtccat
     5401 tcatagaaaa gcaccgacgt ttgatcagtt gagcagtaat tttgaaatcc tggaaacagg
     5461 catcaaggtc atcgatttgc tggctcctta tctgaaaggc ggaaaaatcg ggcttttcgg
     5521 tggggccggt gtcggaaaga cggtcctgat tcaagaattg atccacaacg tagcggaaca
     5581 gcatggcggc atatctgtat tcacgggtgt cggcgagcgt acgcgtgaag gaaacgacct
     5641 ctatcacgaa atgcaagaat ctggagtcgg caaaaagaca gccatggtat tcggtcagat
     5701 gaacgagcca ccaggtgcac gtatgcgcgt cgccttgtcc ggtttgacga tggcagaaca
     5761 tttccgtgat gaagaaaaac aggatgtgct gttgttcatc gataacatct accggtttac
     5821 tcaagctggt tcagaagttt ccgcactatt gggccggatg ccttcagcag taggctatca
     5881 gccgacgtta gcaactgaga tgggccaact gcaggaacga atcagttcca ctaaagatgg
     5941 ttcaatcact tcgattcaag cgatctatgt gccagcggat gactataccg atccggctcc
     6001 tgcaacaaca ttcgcccatt tggatgcaac aacgaacttg gagcgtaaac tgacggaaca
     6061 agggatttat cctgccgttg atccattggc ttcctcttcc agcgcgttat ctccggaaat
     6121 cgtcggagat gaacattacg aagtggccac caaagtacaa cgcattctgc aacgttatcg
     6181 tgaactgcag gatatcatcg ctatccttgg gatggatgaa ctgtccgatt ctgaaaaaat
     6241 tactgttaac cgcgcaagac gaatccaatt cttcttgtcc caaaacttcc acgtagccga
     6301 agcgtttacc ggagtaccgg gatcgtacgt ccctgtagcg gaaactgtcc gtggcttcaa
     6361 agggattgtt gaaggtaaat acgacggagt gccggaagaa gctttccgca acgtcggtac
     6421 gatagaagaa gttctgaaaa aagcgaaaga actaggctac aaaggagggg aatgagatgg
     6481 catacctgca agtgaatgtg gtgacgcctg atggtatcgc ttttcagcat cgtgctaagg
     6541 cggtgaccgc aacgacaacg gatggcggca ttacgatcat gccaaaccat accccgatca
     6601 ttgttcctct ggcgattgct gacctgattg taacgcgtgt gaccgatgaa ttggcgcaga
     6661 accatattgc cgtcaatggc ggcatcatgg aagtgcgtga taatgtggtg aacatcattg
     6721 ccaacagcgc ggaacgcgca cgcgacatcg atatcgaccg tgcggaagtc gcgaaagaac
     6781 gcgccgaaaa acggatggca gaagcgcgtg atttcaaaaa tgaaaaagaa ttccagcgcg
     6841 cgaagatttc gttatcgaaa gcgatcaacc gtatcggtgt ttcgaaaaat agatccaatt
     6901 gaaaaaaaga ggccatcgtt ggcctctttt tttgattccg tttatttcct gacatttaat
     6961 tagtgaagcg gataatatgc atagattatc tgcagaaata gcccgcaatt tcatttcgat
     7021 gtattgtaac atattattat acctgataaa gaatatgaat gtaaacgatt ttcttagtaa
     7081 attcttaggc gatagtctga caaaaaacag cttgttggtt tttttttcaa aatatttatg
     7141 ttagtatagt ttgagaatat gggaggagtg cgtcacttgg acttaacgct gtttaacggt
     7201 ataatagtat tgctatccca tatgatattc attttcattt ctttttggtc gttaaaagcc
     7261 atcagactgg agaaatggat cagaaaggga cacatacgcg aagcaagggt gctctatttc
     7321 tttctgtcga ttgctttagg ctttacggta agtacgtttc tgcttgagtt ggtgacaacc
     7381 tcgcgtaata tcagttactt actgttctca taatcccgct cacagttgca caatagaatg
     7441 tgcctgtaaa ggcagatcag agttccattt ttacaatagt tgcatacaaa catacaaatg
     7501 agatcacaat tgactttcgg agggattttt aatggaaaaa ataattgttc gcggcggtaa
     7561 ccgtcttgaa ggaacagtta aagttgaagg cgcaaaaaac gcagttttgc cgattttggc
     7621 tgctacactt ttggcaagcg aagggcaatc tgtattgacg aacgttccga tactgtctga
     7681 tgttttcaca atgaataatg ttctgaaaca tctgaatgtc ggcgttgaat ttcaagaaaa
     7741 aaacaacacc atcttagcgg atgcatcaaa cgacatcaat tttgaagcgc cctttgctta
     7801 tgtcagcaaa atgcgtgcgt caatcgtcgt tatgggtccg ctattagcac gtctgggtca
     7861 cgcgcgtatc gctttacctg gtggctgtgc catcggaaca cgacctatcg accttcactt
     7921 gaaaggtttc gaagcaatgg gagctgacat caaaatcgag aacggttttg ttgaagcgaa
     7981 agtcgacaga ttggtcggtg cacgcatcta tttggacttc ccgagtgtcg gtgctactca
     8041 gaacatcatg atgggcgcta ctttggcgga agggaccacc atcatcgaaa acgtcgcacg
     8101 cgaacctgaa atcgtcgatt tggctaattt cctgaaccgt atgggcgcga aagtggtcgg
     8161 agccggaaca gaaacaatcc gtatcgaagg cgtttcgtcc atgaaggcaa cagaacattc
     8221 gatcatcccg gaccgaattg aagcaggaac attcatgatt gcagcagcag tcacgaacgg
     8281 gaatgttttt atcgaagatg cgattgccga tcataacaag ccgctgattt cgaaactgaa
     8341 ggaaatgcac gttgctttcc aggaagaaga aaatggtctg cgtgtcatcg gtccacgttc
     8401 tttgaaagcg accgatgtca aaacgatgcc acatccaggg gttcctaccg acatgcaggc
     8461 acaaatgacg attgcgcaat tagtatcaca aggaaccagc acgatgaccg aaacggtatt
     8521 cgaaaaccgt tttatgcatt tggaagagat gcgccgtatg aatgctgatt tcaaagtcga
     8581 agggcagacg gtcctgatt
//
LOCUS       DAOMUJ010000022         8124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2297_length_8124_cov_2.415169, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000022 DAOMUJ010000000
VERSION     DAOMUJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_8124_cov_2.415169"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLT73_01205"
     CDS             complement(<1..785)
                     /locus_tag="PLT73_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM19031.1"
                     /translation="MGKIQIMGLGAGDLEQLPYGVYSRLLAAEFVHLRTKEHPVVENL
                     KAAGVQFESFDDIYESKDNFDDVYRQIADQLLEKAKTQDLIYTVPGHPLVAEDSVKHL
                     LENEAGIEVEIVGGKSFIDDFFQAVAIDPIEGFQLVDGLTFHQDQLSLGNHLIIMQVF
                     NEFVASDVKLKLMEKYPDEHQVALVDAAGTKMQKVTWCPLYEMDRLEGVHNLLSLYVP
                     PLEKDERTKSFELTQAYMDAIVEKDVWVQSQTHESLLPYLKEE"
     gene            complement(811..2430)
                     /locus_tag="PLT73_01210"
     CDS             complement(811..2430)
                     /locus_tag="PLT73_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRM19032.1"
                     /translation="MENNRLKQMMKGALLLSLAAFISKILSAVYRVPFQNMVGNTGFY
                     VYQQVYPIYGIGMTFALSGFPVFLSKMIAETPDSGTRRMILKRSLVILGVFGFILFMG
                     IYGFSGRLALLMGDPELKPILQSVSWMFLWMPILATLRGYFQGNYRMEPTAVSQVAEQ
                     LVRVGAILFAAYLYTQTKGDLYAMGANAMSGATIGAILGSLILLVAYHKERQNDDTLV
                     LDGRLLKQESSDAQWGRLIRRYATEGATICLLSAILVLFQLIDSFTLYNGLLDGGKAS
                     DFAKNVKGIYDRGQPLVQLGMVVGTGFSASFIPMSQAHAQNRQNEFTRSAVSLLRMTS
                     VFSAAAVTGLLAILPNVNNMLFGDREGIAVLSVYILAIFAASIMTAYHSILQSLGQYK
                     ISLVALAAGLTVKFFGNLLLVEPLGTMGASIALIAGLAAMVLVLWMNSERALRDVWFT
                     DHFVRKLLAGCVVLFVTVWALRVGLERYLFTDGDRLTDSVIASVTVCAGVAVFLWYIL
                     KVKLFTLREWVSIPFGKKLLRKLPDKTNDLK"
     gene            complement(2420..5944)
                     /gene="mfd"
                     /locus_tag="PLT73_01215"
     CDS             complement(2420..5944)
                     /gene="mfd"
                     /locus_tag="PLT73_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRM19033.1"
                     /translation="MKDIEKYLKETVNIQTLLSEINSELPQLVAGLSGSGRNLLTNIL
                     FEELQKTIVVLTPNLLQATQVYEDISQMAPEVPLYIFPVEESVAAELAFSSPEYRSQR
                     VETLDFLNSGKKGIVVIPAAGFKKILSPASIWETHCLDFSVGMELDMDSLPEKLVAMG
                     YSRERMVASPGEFSIRGGIIDIYALNEENPLRMELFDTEIDTLRYFDAYTQKSVDSIE
                     TVRILPAQDTIFTKAEIVKQASAVEKKAEKAIKGIKNQEVKESMTRVFSELTDNMKRG
                     ELSKDPQLYTSLVYPEQPKILDYISEDALLIVDDYSRILEVERTIESESSEWKMSKVS
                     EGVCLPSQDFSADIREVIKKSKQHRIYYALFHRGFGNMRFHGVYPFQYRMLNNFFSQM
                     PQVKLEMERWLKQHMTVFVMVPSFERAEKVQRIFADFGVHSYIKGNDPFIENKVNILV
                     GGMANGFELPQEKVVFVTEKELFNKVTKKQPHRQKMTNAERLKSYTELNPGDYVVHVN
                     HGIGLYTGMETIEVGGVHQDYMSIAYQEGAKLFIPVTQIHLIQKYVSSDAKMPKMHKL
                     GGTEWAKTKKKVASKIEDIADELIELYANRESEKGYAYQPDTPEQLEFENAFPYTETD
                     DQLRSTAEIKADMESIKPMDRLLVGDVGYGKTEVAMRAIFKAVQEGKQAAFLVPTTIL
                     AQQHYESLTQRFEEYPFEIGLLSRFRTKKQQDETLAGIRKGTVDIVIGTHRILSKDVV
                     FADLGLLVVDEEQRFGVKHKERLKQLKASVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPVQTYVMEQNNGAVRDAIERELARGGQAFYLYNRVETIEKKAQEIRELVPD
                     VRVAVAHGQMTEVQLENVIMDFIEGRYDVLVTTTIIETGVDIPNVNTLFIENADRMGL
                     SQLYQLRGRVGRTNRIAYAYLMYQPNKMLTEVSEKRLQAIKEFTELGAGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDSVGFDLYSEMLGEAVAAKRGLKAKSKRSIVEIDLSIDAYIP
                     ADYIEDERQKIDFYKRIQRIESVADHDEIDADLIDRFGDYPQEVSDLLEVGLIKNYAE
                     KSQIETIKRQGETVVITFSQEATVRLQGPAVFEALSNIPLKAQINLNNAKLVVSVVIG
                     KLPNRQWLNHLNAFIKACVAETDGTDGK"
     gene            complement(6059..6628)
                     /gene="pth"
                     /locus_tag="PLT73_01220"
     CDS             complement(6059..6628)
                     /gene="pth"
                     /locus_tag="PLT73_01220"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRM19034.1"
                     /translation="MKMIVGLGNPGPKYTDTKHNVGFITLDEWAFQNKEKYNKNKFEA
                     LYIETTVDGEKVLFVKPLTFMNDSGRAVRPLMDYYNIAIEDLVVVYDDMDMPVGKIRL
                     RQKGGAGGHNGIKSLIQHLGTQDFNRIRIGVGRPQHNMTVVQHVLSPFPKDEHAEMLH
                     SVKESVDAIDHFIKGNNFLETMNQFNKRG"
     gene            7018..7995
                     /locus_tag="PLT73_01225"
     CDS             7018..7995
                     /locus_tag="PLT73_01225"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM19035.1"
                     /translation="MAELSGNTGQKIILVGDGAVGSSYAFALVTQNIGHELGIIDINK
                     AKTEGDAIDLSHALAYTSPKKIYAASYADCHDADIVVLTAGAAQKPGETRLDLVSKNL
                     RIFKSIIGEIMASGFDGIFLVASNPVDILTYATWKFSGLPANRVIGSGTSLDSARFRQ
                     SIAALVGVDTRNVHAYILGEHGDTEFPVWSHANVGGLQIYEWVKQTSAVDEKALVDVF
                     FKVRDAAYEIIEKKGATFYGIAVALARITKAILNDENAIMPLSVYLDGEYNHKDVFIG
                     VPAVINRSGIRNIIEIPLSDAEQEKMDLSATTLKKVLEEGFASLEADAK"
BASE COUNT         2039 a         2092 c         1741 g         2252 t
ORIGIN      
        1 cattcttcct tcagataggg caataagctt tcgtgcgtct gcgattgcac ccaaacatct
       61 ttttctacga tggcatccat gtaagcttgg gtcaactcaa agcttttcgt tcgctcatct
      121 ttctcaagcg gtggaacata caaggagagc aagttgtgca cgccctctag cctgtccatc
      181 tcgtacaacg ggcaccaagt cactttctgc atctttgtcc ctgccgcatc gaccaatgcc
      241 acttggtgtt catccggata tttctccatc agcttcaatt ttacatcact ggccacaaat
      301 tcattgaaaa cttgcatgat gatcaggtga ttgcctaagc tcagctggtc ctggtggaag
      361 gtcaacccat ccaccaattg gaaaccctcg atcggatcga tggctaccgc ctgaaagaaa
      421 tcgtcgatga aactcttccc cccgacgatt tccacttcga tccccgcctc gttctccagc
      481 agatgcttga cggaatcctc cgcaaccaac gggtgtcccg gtactgtata aatcagatcc
      541 tgcgtttttg ccttttccaa taactgatct gcgatctgac ggtatacgtc atcaaaatta
      601 tccttcgatt catatatgtc atcaaaactt tcaaactgca cgcctgcagc cttcagattt
      661 tcaacgaccg ggtgctcttt ggtccgcaga tggacgaatt cggctgccag caagcgactg
      721 tatacgccgt acggcaattg ttccaagtct ccggctccaa gccccattat ttgaatttta
      781 cccatacatt tctccttctg taacgctttt ctattttaag tcgttcgttt tatccggcaa
      841 tttgcgcagc agttttttcc cgaacggaat acttacccac tcccgtaatg taaagagttt
      901 cacttttaga atataccata agaatacggc cacaccagca caaactgtca cagaggctat
      961 aacggaatcc gtcagccggt ctccatcagt gaagagatac ctttccagac cgacccgcaa
     1021 cgcccatacc gtaacaaaaa ggacaacgca tccagccagc agtttgcgga caaagtgatc
     1081 cgtgaaccag acatcccgca aggcgcgttc actgttcatc caaagcacca acaccatggc
     1141 agccaaaccg gctatcaaag caatgcttgc tcccatcgtc cccagcggtt ccaccaacag
     1201 caaattgccg aagaatttga ccgtaagccc cgctgccagt gcaaccaaag aaatcttgta
     1261 ctgacccaaa ctttgtagga tggaatgata ggctgtcatg atcgaagccg caaaaatggc
     1321 caaaatgtat acggacagaa cggcaattcc ttcccggtcc ccaaaaagca tgttgttgac
     1381 attcggcaaa atagccaaca atcccgttac cgcagctgct gaaaaaacgc ttgtcatccg
     1441 cagcaacgac acggctgatc gggtgaattc gttttggcga ttttgtgcgt gcgcttggct
     1501 catcggaatg aagctggccg aaaaaccagt ccccaccacc atgcccaact gcacaagcgg
     1561 ctgcccacgg tcatagattc ccttgacatt tttggcaaag tcgctggctt taccgccgtc
     1621 caacaagccg ttgtagagtg taaacgaatc aatgagctgg aacaggacca agatagcgct
     1681 caacagacaa atcgtcgccc cttcagtcgc gtaacggcgg atcagacgtc cccattgggc
     1741 atccgacgat tcctgtttca gaagtcgccc atccagcacc agcgtatcat cattctgcct
     1801 ttctttgtga taggcgacca acaggataag actgccaagg atcgcaccga tagtcgcacc
     1861 gctcatggca tttgcgccca tcgcatagag atcccctttt gtttgtgtgt acagatacgc
     1921 cgcaaaaaga atcgcaccga cacgcacgag ctgctccgct acttgcgaaa cagctgtcgg
     1981 ctccatgcgg taattgcctt ggaaataccc ccgcaatgtt gccaatatcg gcatccacaa
     2041 aaacatccag gataccgatt gcaaaatcgg cttcagctcc ggatctccca tcagtagcgc
     2101 cagtcgacct gaaaagccat aaatccccat gaacaggatg aatccgaaga cgccaagtat
     2161 gaccaggctg cgtttcaata tcatcctgcg ggtcccgcta tccggcgttt ccgcaatcat
     2221 cttcgaaaga aataccggaa aacccgatag agcgaacgtc ataccgatac catagatagg
     2281 ataaacttgc tgatagacat aaaagcctgt gtttcccacc atattctgga acggcacacg
     2341 atacaccgca ctgagtattt tgctgatgaa agcggctaaa gacagcaaca gcgcaccttt
     2401 catcatctgc ttcaagcgat tattttccat ctgttccatc cgtttcagct acgcaagctt
     2461 tgatgaaagc attcaaatga ttcagccatt ggcggttcgg taatttccca ataaccacac
     2521 tgacaaccaa tttggcatta ttcagattaa tctgcgcctt caatggtatg ttgctgaggg
     2581 cttcaaacac tgcaggcccc tgcaagcgga cagtcgcttc ttgagaaaag gtgatgacga
     2641 ccgtttcgcc ttggcgcttg attgtttcta tttgactctt ttctgcgtaa ttttttatca
     2701 gaccaacctc caacaaatcg gaaacctctt gcggataatc accgaagcgg tcaatcagat
     2761 ctgcatcgat ttcatcatga tcggcgacac tttcgatacg ctggatccgt ttgtagaagt
     2821 cgattttctg ccgttcatcc tctatataat cggctggtat ataagcatct atactcaaat
     2881 caatttcgac gatcgaacgc ttcgatttcg ctttcaatcc acgtttggcc gcaactgctt
     2941 ctcccagcat ctccgagtag aggtcgaatc caaccgagtc gatgaagccg tgctgttgcg
     3001 cacccaatag gttcccagct ccacgtatcg acaagtcacg catagcaatc ttgaagcccg
     3061 cgcccagttc cgtgaattct ttgatcgcct gcagtctttt ttcactgact tcagtcagca
     3121 tcttgttcgg ctgatacatc agataggcat aggcaatccg atttgtccta ccgacacgtc
     3181 cgcgcaattg atacagttgc gacaagccca tccgatcggc attttcgatg aagagcgtat
     3241 tgacattcgg gatgtctaca cctgtttcta taatggttgt ggtgaccagc acatcgtagc
     3301 ggccttcgat gaaatccatg atgacatttt cgagttggac ttccgtcatc tggccatgcg
     3361 ccactgcgac acggacatcg gggacgagtt cgcggatctc ctgggctttc ttctcgatgg
     3421 tttccacccg attgtagagg tagaaagcct gacctccgcg ggccaactct ctttcgatcg
     3481 catcccggac ggctccgtta ttctgttcca tgacatatgt ctgaaccgga tagcggtttg
     3541 ccggcggcgt ttcgatgacg gagagatctc tgacccctag catcgacata tgcagagtac
     3601 gcggaatcgg tgttgccgtc aacgtcaata catccacaga agctttcagt tgtttcaagc
     3661 gctctttatg tttcacacca aatcgttgtt cttcgtcgac caccaataat cccaaatcag
     3721 cgaaaacaac gtccttcgat aagatccggt gcgtgccgat aacgatatca actgttcctt
     3781 tccggatgcc ggcaagtgtt tcatcttgct gtttcttcgt gcggaagcgg ctcaacaaac
     3841 cgatttcaaa tgggtactct tcaaatcgtt gggtcaacga ttcatagtgc tgttgcgcca
     3901 aaatggtcgt agggacaagg aaagcagctt gttttccttc ctgcactgcc ttaaagatgg
     3961 cccgcatcgc aacttccgtt tttccgtaac ctacatcacc gacaagcaag cgatccatcg
     4021 gcttgatgct ctccatgtcc gccttgattt cagcagtact acgcagttgg tcatccgttt
     4081 cggtgtaagg gaaggcgttc tcgaattcga gttgctccgg cgtatccggt tgataggcgt
     4141 accctttttc tgattcgcga ttggcgtaca gttcgatcaa ttcatcagcg atatcttcga
     4201 ttttcgaagc aactttcttt ttcgttttcg cccattccgt cccacccaat ttgtgcattt
     4261 tgggcatttt ggcatcggag gagacatatt tttggatcaa atggatttgc gtcacaggaa
     4321 tgaataattt ggcgccctcc tggtatgcga tcgacatgta gtcttggtgg acacctccaa
     4381 cttcaatcgt ttccatgccc gtgtagagtc ctatcccgtg atttacgtga acgacataat
     4441 caccggggtt cagttccgtg taactcttca gtctctcggc attcgtcatt ttttggcgat
     4501 gcggctgttt cttagtgact ttgttgaaaa gttccttttc agtgacgaat accacttttt
     4561 cctgcggcaa ttcgaatccg ttcgccattc cgccgaccag gatattcacc ttattctcga
     4621 taaacggatc gttgcctttg atgtagctat ggacgccgaa atccgcgaat atccgctgca
     4681 ccttttcagc acgttcgaaa gacgggacca tgacaaaaac cgtcatatgc tgcttaagcc
     4741 aacgttccat ttccagcttg acttgcggca tctgactgaa gaagttgttc agcattcggt
     4801 attggaaagg atagactccg tggaagcgca tatttccgaa gcccctatgg aacagtgcgt
     4861 aatagatgcg atgctgtttg gattttttga tcacctcgcg gatatccgcg ctgaaatcct
     4921 gtgaaggcaa gcatacgcct tccgacactt tggacatttt ccattccgat gattcgcttt
     4981 ctattgtcct ttcaacctca agtatgcggc tgtaatcgtc cacgatcagc agcgcatctt
     5041 cggagatata gtccaaaatc ttaggctgtt ccggatagac taagcttgtg tagagctgcg
     5101 gatccttaga cagttcgcca cgtttcatat tatccgtcag ttcggagaat acacgagtca
     5161 tactttcttt gacttcttga ttcttgatac ctttgatggc cttctcagcc tttttctcta
     5221 ccgcactggc ctgcttgact atctccgctt tcgtgaaaat tgtgtcctgc gccggcaaaa
     5281 ttcggacagt ttcgatagag tcgacggatt tttgggtgta ggcatcgaaa taacgcaatg
     5341 tgtcgatttc ggtatcgaac agttccattc gcaaggggtt ctcttcgttc agggcataga
     5401 tgtcgatgat gcctccgcga atgctgaact cacctgggct ggcaaccatc cgttctcgtg
     5461 aatatcccat tgcaaccaat ttctcaggca agctgtccat atccaactcc atgccaacgg
     5521 aaaaatctag acaatgcgtt tcccagattg aagctggaga gaggattttt ttgaatccgg
     5581 cagctgggat cacaacgatc ccttttttgc cgctattcag aaaatccaac gtttcaaccc
     5641 gttgactgcg gtattcgggt gatgaaaacg ccagttccgc cgcaaccgat tcctcaaccg
     5701 ggaagatata caaaggaacc tccggcgcca tttgactaat gtcttcgtac acttgggtcg
     5761 cttggagcaa attcggggtc aagacaacaa tcgtcttctg caattcttcg aacaggatat
     5821 tcgtcagcag attgcggcct gatccggaaa gccctgctac caactgtggt agttctgaat
     5881 tgatttcaga caataacgtc tgtatattta ctgtttcctt taaatatttt tctatatctt
     5941 tcattgatat tctcctcatt aatcatatgt tctgcagaag aaaaggttat cgctttcatc
     6001 tcgattcatt attgcagatt gtacagcaga cacaaaagag gtccagctaa agctgaacct
     6061 aacctctttt attgaattga ttcattgttt ccaaaaaatt gttccctttg atgaagtggt
     6121 cgattgcatc cacactttct tttacggagt gcaacatttc ggcgtgttca tctttcggaa
     6181 aaggactcaa aacgtgctga acgaccgtca tattgtgttg aggtcttccg accccgatgc
     6241 ggattcggtt gaaatcctga gtacctaggt gctggatcaa gcttttgatg ccattatgcc
     6301 cgccagctcc acctttttgt cgaagcctta ttttccctac aggcatatcc atatcatcgt
     6361 atacgacaac cagatcttcg attgcaatgt tgtagtagtc catcaatggt ctcaccgctc
     6421 ttcccgaatc attcatgaat gtcagcggct tgacgaataa aactttttca ccgtctacgg
     6481 tggtctctat gtataacgct tcaaatttat ttttattgta tttctcttta ttctgaaagg
     6541 cccactcgtc taaagtaata aagccgacat tatgcttcgt atcggtatac ttaggacctg
     6601 ggttccccaa tccaacgatc attttcattt atattcccct atttttccag ttgttgttca
     6661 ctccgtctgg gaacaagtac cttcttccct ttacgccaaa aaaggccgca cgaatttcat
     6721 ccgcacagtt tacatgcact tagtatacca tactcctcgc atattcgtaa gaaatcggat
     6781 tggcatgggc aaaaaatatt atgaatgttg tgaacaaact cgcaaacccc cattgtaaat
     6841 agaagtgttt tattttataa tatatttata tatcatttca caggttgttc actttctatc
     6901 actgtcgcgc aatcatcgtt ggttctgaaa atctttgccg atcgggatag gcccaaagag
     6961 taatcacctt tgtttagtgt tataatagaa ccagcaaata gcgagaggag cattataatg
     7021 gcagaattga gtggaaatac gggacaaaaa attattttag tcggtgacgg cgctgttgga
     7081 tcaagctatg cgtttgcatt ggttactcaa aacatcggtc atgagttggg tatcatcgat
     7141 atcaacaaag caaaaacaga gggagatgcg atcgatcttt cccacgcttt agcttatact
     7201 tcaccgaaga aaatttacgc agcatcgtac gcagattgtc acgacgctga catcgtagta
     7261 ttgactgcgg gagctgccca aaaacctggc gaaacccgtc tcgatctagt aagcaaaaac
     7321 ttgcgcatat tcaaatcaat catcggagag atcatggcta gtggattcga cggcattttc
     7381 ttggtcgcat ccaatccagt ggatatcctt acttatgcaa cttggaaatt ttcaggactt
     7441 cccgctaacc gtgtcatcgg ttcaggcact tccctggaca gcgctcgatt ccgccaaagc
     7501 atcgctgcac ttgtaggcgt agatacccgt aatgttcacg cctatatctt aggggaacac
     7561 ggcgacaccg aattccccgt gtggtcccac gctaacgttg gcggcctgca aatttacgaa
     7621 tgggtgaagc aaacttctgc tgtcgatgaa aaagctttag tcgacgtctt cttcaaagta
     7681 cgcgacgctg cgtatgaaat catcgagaaa aaaggagcga ctttctacgg aatcgctgta
     7741 gccttagccc gtatcacaaa agcaatcctg aatgacgaaa acgcgattat gcctttatcc
     7801 gtctatttgg atggagaata caaccacaaa gatgtcttca tcggcgttcc ggcagtcatc
     7861 aaccgttccg gtatccgcaa tatcatcgaa attcctttat ctgatgcaga acaggaaaaa
     7921 atggatctat ccgctacaac actgaaaaaa gtgttggaag aaggattcgc cagcctcgaa
     7981 gcggacgcaa aataatcgtt tccgatttca aacactcgtt ggtcctttgg ggacgacggg
     8041 tgttttttgt tgtggtttcg atgatttggg gtttggtttt ttaggggctg ttgggggctg
     8101 ttgggggctg ttgggggctg ttgg
//
LOCUS       DAOMUJ010000023         8023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2334_length_8023_cov_2.803589, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000023 DAOMUJ010000000
VERSION     DAOMUJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_8023_cov_2.803589"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PLT73_01230"
     CDS             complement(<1..1489)
                     /locus_tag="PLT73_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM19036.1"
                     /translation="MKISKRLKMMKEKQKLQCKSRGNILLLHCFSLLVTVFVVHLFLQ
                     WTQNDLDAGLVVNFIFAWHTEKFLISTGVLVMLGLWLWALVGNIRLANALLVLSGGIL
                     GMATYEKMLQRNEPVYPSDLKMLTEATFLLEMLNGRTLAALSLVFLLFLAFVLYSLRQ
                     AKSKKTVKLGWKSRVLILITTSMALGYAGQFQQEGNLLKKAYDRTAYWIPYSQQMNYY
                     NTGFVAGFLYNLSAAPMELPADYSQEKIDVLKATYQQLADEINTNRTVALPETNVIYI
                     MNESFADPLELVGLDLQTDPIPFTRALMDTSYSGELLSQGYGGGTANIEFEALTGFSM
                     EPFAANITTPYTQFLSSRDNFPSIVSRLEQAGFRTTAIHPYNTTMYKRLENYEALGFD
                     TFLYEDTMANTAKLDTNPYISDAAAYAEITAILKASAEKDFIHLVTMQNHTPYQDKYT
                     AIPSAAETGIASQSIRNYLQDLQYSDQALAELLATLQEWDEPTIVV"
     gene            complement(1515..2348)
                     /gene="nadE"
                     /locus_tag="PLT73_01235"
     CDS             complement(1515..2348)
                     /gene="nadE"
                     /locus_tag="PLT73_01235"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HRM19037.1"
                     /translation="MRPLQKEIIAALRVKPEIDPKEEIRTSIDFMKDYLAAHPFLKAF
                     VLGISGGQDSTLTGRLAQLAIQEMRDETGDESYQFIAVRLPYGIQFDEHDAQAALDFI
                     SPDQKLVVNIKEMADAALDALEEAGLTITDFNKGNIKARQRMIVQFAIAGDSSGAVLG
                     TDHAAESVTGFFTKFGDGAADLLPIWRLNKRQGRQLLQELGAPKNLYEKVPTADLEEN
                     RPALPDEVALGVTYEMIDAYLEGKEIPEKDAEVIEGWYTKTEHKRHLPITVYDSFWKE
                     A"
     gene            complement(2378..3862)
                     /locus_tag="PLT73_01240"
     CDS             complement(2378..3862)
                     /locus_tag="PLT73_01240"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM19038.1"
                     /translation="MSYDYNDDSLALHTDLYQINMMKTYWDEGITEKHAIFELYFRKY
                     PFNNGYAVYAGLERFVSYLQNLRFTDTDIAYLREHVGYDEGFLEYLKNFRFSGTIRSA
                     LEGDLVFANEPIVQVEGPLAECQLIETALLNIVNFQTLIATKAARLRYVCNDEPLMEF
                     GSRRAQEMDAAIWGTRAAYIAGFNATSNVRAAKMFGIPASGTHAHSMVQVYRNDYDAF
                     MAYAHSHKDCVFLVDTYDTLKSGVPNAIKVAKEMGDKINFLGVRLDSGDMAYISKKVR
                     QQLDEAGFTDAKIYASNDLDELTILNLKMQGAKIDVWGVGTKLITAYDQPALGAVYKL
                     VSIADENGQMVDTMKISSNAEKVSTPGKKQVWRITRKRDGKSEGDYIALWEEQPDQES
                     ELYMFHPVFTYINKTITDFEARPILQDIIVEGKLVYELPSLQEVKAFSEAQMSGLWDE
                     YKRILNPEDYPVDLSQKTYDHKMATIKEIKKQIKEEASLLESTK"
     gene            complement(4102..5496)
                     /locus_tag="PLT73_01245"
     CDS             complement(4102..5496)
                     /locus_tag="PLT73_01245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM19039.1"
                     /translation="MNQTGEWNKARKIIIGIVDMLLFIGSLLLSFYLKFGTDIPWINF
                     DAFQGSIVYVSVIFIIVNILFGTYIFYNKSISDFLFITVIGEFVLSLIIMALTFAGRW
                     LAFPRSVILINFFVGTIILFIFRVMVFKMYQRVSGSKRVMIVGMEKEVFAAVDNFTNT
                     KSIRHVVTHVVLSDYYENVLRHLEEMDIVYLASQIEESEKLKIYDLLTSKEKKMFLNT
                     SFENLIMVNPNMMNIEDESIIEVSPFRIPAEDGLMKRIIDIAVALIGIVITSPIMAVT
                     AILVKKSSEGPVFYKQTRITKDGKEFEILKFRSMGVTAEKESGPVLATSNDVRVTKVG
                     KYLRSLRIDELPQLFNVLLGDMSLVGPRPERPFFVDQFKELNPHYYLRHNVRAGITGY
                     AQVYGKYASDFNSKLNFDLIYIKKYSLILDLKIMLQTVKILFDKVSSRGVDESEREIL
                     TEQEIEARGIRVIY"
     gene            complement(5730..6989)
                     /locus_tag="PLT73_01250"
     CDS             complement(5730..6989)
                     /locus_tag="PLT73_01250"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRM19040.1"
                     /translation="MAAKESIKKVVLAYSGGLDTSVIIPWLKENYNNCEVIAVVGDVG
                     QGDDYSRLEEKAINCGASKLYVADLKKEFVEEFIWPTLQAGAKYEGQYLLGTSFARPI
                     IAKRMVEIAHLENADAIVHGATGKGNDQVRFEVGIREFDPNITIIAPWREWELTSRED
                     EFAYAAAHNVPLPITLETNYSKDKNLWHLSHEGLDLEMTANEPDYDKILEMCVTPEKA
                     PDEATYVTLEFKQGVPVSLNGEAMDGIALIEKLNVIAGANGVGIIDIVENRLVGMKSR
                     GVYETPAGTVLYHAHDKLEQLCLDKDTYQYKQLLANKYAELVYNGLWYTPLRKALGAF
                     VDETQQNVEGFVKMKLYKGNITDAGVVSAKSLYSESFATFESDDVYNHTDAAGFIRLF
                     GLPTSIRVMKEQAEGQATHEELKDLLK"
     gene            complement(7228..>8023)
                     /locus_tag="PLT73_01255"
     CDS             complement(7228..>8023)
                     /locus_tag="PLT73_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM19041.1"
                     /translation="PGLLPGMAGGYLAVQANAGFLGGLIAGFLAGYIMVYLKKAFKGL
                     PRTLDGMKTILLYPVFGLLITGLLMYFLVGPVFSTINTAMITFLENLGTGNAVLLGAV
                     LGGMMAIDMGGPFNKAAYAFSIGIFTDTGDGSLMAAVMAGGMIPPLAIGIATLLFKNK
                     FTDIEQKSGLSNILLGLSFITEGAIPFAAADPMRVIGSSIVGSAIAGGLTQLWAVSIP
                     APHGGLFVITLANRPLMFLLALLIGTVVSALMLGLWKQDKTQEVVK"
BASE COUNT         2024 a         1996 c         1759 g         2244 t
ORIGIN      
        1 aaacaacgat ggttggctca tcccactcct ggagggtagc cagcaattcc gccaaggctt
       61 gatcgctgta ctgaagatcc tgcagatagt tccggatcga ctggctggct atacctgttt
      121 cagcagcaga agggatagct gtgtatttat cctggtaagg ggtatggttc tgcatcgtca
      181 ctaagtggat aaaatctttt tctgcgctgg ctttcaggat agccgttatt tcagcgtaag
      241 ctgctgcatc cgaaatatac ggattggtgt ccaactttgc cgtattcgcc atcgtgtcct
      301 cgtagaggaa ggtgtcaaaa cccaaagcct catagttctc cagccgcttg tacatggtcg
      361 tattgtaggg atggatggcg gtagtcctga agcctgcctg ttccaaacgg gatacgatgg
      421 aagggaaatt gtctcgggaa gacagaaatt gcgtatacgg cgttgtgatg tttgcggcga
      481 aaggttccat cgaaaagccc gtcaaagcct caaattcgat attggcggtc ccgccgccat
      541 agccttggga caacagctcg ccactgtagc ttgtgtccat caaagctcgc gtgaacggaa
      601 tcggatcagt ttgaagatcc aagcccacca actcaagcgg gtccgcaaaa ctttcattca
      661 tgatgtagat gacattcgtt tcaggcaaag ctaccgttct atttgtattg atttcgtcag
      721 ccagttgttg ataggttgct ttcaacacat cgattttttc ctgcgaatag tccgccggca
      781 gctccattgg cgcagccgaa aggttgtata ggaagccggc aacaaagccg gtgttgtagt
      841 aattcatctg ttggctgtac ggaatccaat aagccgtccg gtcataggct ttcttcagca
      901 ggttgccttc ctgctggaac tggccggcat agccgagcgc catgcttgtt gttatcagaa
      961 tcagcacgcg cgatttccat cccaatttaa cggttttctt ggacttggcc tgacgcaggg
     1021 aataaagcac aaatgccaga aacagcagga acaccaggct caatgccgcc aacgtccgtc
     1081 cgttcagcat ctccaaaagg aaggtggcct ccgtcagcat cttcaagtcg ctcggataaa
     1141 cgggctcgtt gcgctgcagc atcttctcat aggtcgccat cccgaggatt cctcccgaca
     1201 gcaccagcaa ggcgttcgcc aagcgtatgt tccctaccag cgcccaaagc catagaccca
     1261 gcatcaccaa tacgcccgta ctgatcaaga acttctcggt atgccaagcg aatataaaat
     1321 tcacgaccag tccggcgtcc agatcgtttt gagtccattg aagaaaaaga tggacaacga
     1381 atacggtcac caacaagctg aagcagtgca agagcaggat attccccctg cttttgcact
     1441 gcagcttttg cttttctttc atcattttaa gtcgtttgct tattttcact gcaaaacccc
     1501 actatctgag cagattaggc ttctttccaa aaactatcgt agactgttat cggcaaatga
     1561 cgtttatgtt ctgtcttcgt gtaccagcct tcgatgactt cagcgtcctt ttccggtatt
     1621 tcctttcctt ccagatacgc atcgatcatc tcgtatgtca cgcccaaagc tacctcgtca
     1681 ggcaaagcgg gacgattttc ttccaagtcc gctgtcggta ctttttcata gagatttttc
     1741 ggcgcaccca attcctgtaa tagttggcga ccttgacgct tattcaaccg ccaaatcggc
     1801 aataagtcgg ctgcgccgtc cccgaatttt gtgaagaaac cggtcacgga ttctgccgcg
     1861 tgatccgttc ccagaacagc accgctgctg tcgccggcta tcgcgaactg aacgatcata
     1921 cgttgacgtg ctttgatgtt gcctttattg aagtcggtga tcgtaaggcc cgcttcctca
     1981 agcgcatcga gtgcagcgtc agccatttcc ttgatgttga cgaccaattt ttggtcaggt
     2041 gagatgaaat caagcgcagc ctgagcatcg tgttcatcaa attgaatacc gtaagggagg
     2101 cgcacagcga tgaattggta actttcgtcg ccggtttcgt cacgcatttc ctggatcgcc
     2161 aactgggcca atctgcccgt caaagtggag tcttggcctc cgctgattcc caaaacgaat
     2221 gccttcaaga atgggtgtgc cgccaaataa tctttcatga aatcgatgct ggtgcggatt
     2281 tcctctttag gatcgatttc cggttttact cttaaagcag caataatttc tttttgtaat
     2341 ggacgcatcg ctattccccc ttgattgatc tgattggtta tttagtgctt tccaataatg
     2401 atgcttcttc ttttatttgc tttttgattt ccttgattgt agccatctta tgatcgtatg
     2461 tcttctggga caagtcgacc ggatagtctt ccggattcag gatacgtttg tattcatccc
     2521 acaagccgct catctgcgct tccgagaatg cttttacttc ttggagcgac ggcaattcat
     2581 atacaagttt accttctacg atgatatcct gcagaatagg gcgcgcttca aagtcggtga
     2641 tggttttgtt gatgtaagtg aagacaggat ggaacatata cagttccgac tcttgatcgg
     2701 gttgttcttc ccaaagcgca atgtagtctc cttccgactt accgtcgcgc ttgcgggtga
     2761 tccgccatac ttgtttcttg cccggtgtgg agactttttc ggcattgctg gagattttca
     2821 tcgtatccac catttggccg ttctcatccg cgatcgagac taatttatag accgctccaa
     2881 gcgcaggttg gtcataagcg gtgatcagtt tggtgccgac accccagaca tctatttttg
     2941 cgccttgcat cttcaggttc aagattgtca gttcatccag gtcgttcgat gcgtagattt
     3001 ttgcatcggt aaaacctgcc tcgtccaatt gttggcggac tttttttgaa atgtaggcca
     3061 tgtctccgct gtctaggcgc acgccgagga aattgatctt atcgcccatt tccttcgcca
     3121 ctttgatggc atttggcaca cctgatttca acgtatcgta ggtatccaca aggaagacac
     3181 aatccttgtg ggaatgcgca taagccataa aggcatcata atcattgcgg tagacctgca
     3241 ccatcgaatg cgcatgggta cctgaggcgg gaataccgaa cattttagca gcccggacat
     3301 tgctcgttgc attgaagccg gcgatatagg ctgcacgggt gccccaaatg gcggcatcca
     3361 tttcctgcgc tcttctggaa ccaaactcca tcagtggctc atcgttacag acatagcgca
     3421 gtcgggcggc ttttgtcgct atcagcgtct ggaaatttac gatattcaat aaggctgttt
     3481 cgatcaattg gcattccgct aatggacctt ccacctggac gatcggttca ttcgcgaata
     3541 cgagatcacc ctccaaggcg gaacggatcg tgcccgagaa acggaaattt ttcagatact
     3601 ccaaaaaacc ttcatcgtaa ccgacatgtt cacgcaaata ggcgatgtcg gtgtccgtaa
     3661 agcgcaggtt ctgaaggtag gatacgaagc gttcaagccc cgcatagacg gcatagccat
     3721 tattgaacgg atacttccgg aagtacagtt caaaaatggc atgtttttcg gtaatacctt
     3781 catcccaata agtcttcatc atgttaattt ggtacaaatc agtatgcaaa gctaaactat
     3841 catcgttata atcataactc atccaactca ctcatttcat ctttgatcgt acgcctccgc
     3901 attctgtcgc ggtacagcag aaggaagtgc tctatttacc tattgtaacg caattcctca
     3961 cacatttccg cttttttgtc caaaatccat cagattgatt atagtgatcg gcccgcattc
     4021 cggcaaatac aaccggcatt tctgcgtaaa caaaaaacca tcctggcggg cggaaaaatg
     4081 cccgccagga tggtttgcta tttaataaat gactctgatg ccgcgtgctt caatttcttg
     4141 ctcggtcaga atctcccgtt cgctttcatc gactccgcgg gatgatactt tatcaaacaa
     4201 gattttgacg gtctgcagca tgattttcag atccagtatc aaggaatatt tcttgatgta
     4261 gatcagatcg aagttcagct tgctgttgaa atcggaagcg tacttcccat acacttgcgc
     4321 atatccggta attccggcac gcacattgtg gcgcagataa tagtgcgggt tcagttcctt
     4381 gaattgatca acgaagaaag gacgttccgg ccggggaccg accagggaca tatcgcccag
     4441 caatacgttg aacaactgcg gcagctcatc gatgcgcaga gagcggagat atttaccgac
     4501 tttcgttacg cgcacatcgt tgcttgttgc caaaaccggt cctgactctt tctcggcggt
     4561 cacacccatg ctgcggaact tcagaatttc gaattccttg ccatctttgg tgatgcgtgt
     4621 ctgcttgtag aacacgggac cctcagaact cttcttcacc aagatggccg taacggccat
     4681 aatcggcgag gtaatgacga taccgatgag cgcaactgcg atatcgatga tccgcttcat
     4741 aaggccatct tctgccggaa tacggaacgg ggacacttcg atgatacttt catcctcgat
     4801 attcatcata ttcggattga ccatgatcaa gttttcgaaa ctggtattca gaaacatctt
     4861 tttctctttg ctcgtcaaca aatcatatat tttcagtttt tcggattctt caatctgact
     4921 cgctaagtag acgatatcca tttcttccag gtgccgcaga acattttcgt agtaatcaga
     4981 caaaacaaca tgtgtcacga catggcgtat gctttttgta ttcgtaaaat tgtcgactgc
     5041 cgcgaatact tccttttcca tcccgacaat catgacccgt ttgctgccgc tcacgcgttg
     5101 gtacattttg aataccatca cgcggaagat gaacagtatg attgttccca caaagaagtt
     5161 gatcaggatg accgaccgcg ggaacgccaa ccaacgcccg gcaaaagtca gcgccatgat
     5221 gatcaatgaa aggacgaatt ctccgatgac cgtgatgaac aagaagtcac tgatcgactt
     5281 gttataaaag atatacgtac cgaacaggat gttgacgatg atgaagatca ccgatacata
     5341 gacgatgctt ccctgaaagg cgtcgaagtt tatccatggt atgtcggttc cgaattttaa
     5401 ataaaaagat agcagcaatg atccgatgaa caacagcata tcaacgatgc ctatgataat
     5461 tttacgggct ttattccatt cacctgtttg attcacgctt aatctcctcc tccttagact
     5521 tgcttgatat gaatgcattc attttatcat agtttattct tttgcgatat gaaagtttag
     5581 acagacaaca cccctccaac cgtgtacaag cgcatatgat taagggatga gtatcagaca
     5641 actgaaaaag aacgtctaca tcagcttcta ttgcctgatg tggacgttcc tgagcggatc
     5701 agttgtcgac aaacactaat accggttgat tattttaata ggtccttcaa ttcttcatgt
     5761 gtcgcttggc cttcagcctg ctctttcatc acgcggatcg atgtaggcaa gccgaacagg
     5821 cggataaagc cggctgcatc ggtatggttg tatacatcgt cgctttcgaa tgttgcaaag
     5881 ctttcgctgt acaatgattt cgcgctgaca acgccagcat cagtgatgtt gcctttatac
     5941 agcttcattt ttacgaagcc ttcaacattt tgttgcgttt cgtcaacaaa ggcacccaat
     6001 gctttgcgca atggcgtata ccacaagccg ttgtaaacaa gctccgcata tttgttggca
     6061 agcaattgtt tatattggta ggtatctttg tccaggcaaa gttgttccag tttgtcgtgg
     6121 gcatggtaca gcacagttcc ggctggcgtt tcataaacgc ctcttgattt catgccgacc
     6181 aagcggtttt ccacgatatc aatgatgccg acgccgttcg caccagcgat cacgttcaat
     6241 ttctcaatca atgcgatgcc gtccattgct tccccgttca gactgacggg aacgccttgc
     6301 ttgaattcca gagtcacata agtcgcttcg tcaggcgctt tctcaggcgt cacacacatt
     6361 tccaggattt tatcgtaatc aggctcattg gctgtcattt ccagatcaag gccctcatgc
     6421 gatagatgcc acaagttttt atcttttgag tagtttgttt ccaaagtaat cggaagcggc
     6481 acgttatgtg cagcagcata cgcaaattca tcttctctgg aagtcagttc ccattctctc
     6541 caaggagcga tgatggtgat gttcggatcg aattctctga tcccgacttc gaaacggacc
     6601 tggtcattcc ctttccctgt tgccccgtgg acgattgcgt cagcgttttc caagtgcgca
     6661 atctctacca ttcttttggc aatgatcgga cgcgcaaaag aagtccccaa taaatattgt
     6721 ccttcgtact tagcgcctgc ttgcaatgtc ggccaaatga attcttcgac gaattctttt
     6781 ttcaagtctg caacatacaa tttcgatgcg ccacagttga tcgctttttc ttccaatcgg
     6841 ctgtagtcgt caccttgtcc tacgtctcct actacggcga ttacttcaca gttgttgtag
     6901 ttttctttta accaagggat gatgactgac gtatccaatc cacctgagta agccaaaacc
     6961 acttttttga ttgattcctt tgctgccatg cttttacctc cttatacaga ttcaatatcc
     7021 gttgctttta tgcatgaact gatatctaca ttttcgttta ttcaacaata tgcattatag
     7081 catatctgcc atgaaaatca tcccattttt ttacattatt ctcatttatc tgtcatactc
     7141 ttttcattta tagggcttat tttccgaaca gcaaaaagag aacagctagg gaaataccct
     7201 gtctgttctc taaatgagtg attcaattta ttttacgact tcttgtgtct tatcttgttt
     7261 ccaaagacct aacatcaagg ctgaaacgac tgtaccgatc aataaagcca acaagaacat
     7321 tagtggacgg ttggccaatg tgattacgaa caagccacca tgtggtgcag ggatcgaaac
     7381 ggcccatagc tgcgtcaaac cacctgcaat cgctgaacca acgatagatg atccgataac
     7441 gcgcatcgga tctgctgcag cgaatggaat cgcaccttca gtgatgaagg acagacccaa
     7501 caagatgttg gaaagaccgg atttttgttc gatatccgta aatttgtttt tgaaaagcaa
     7561 ggttgcgata ccgattgcca atggaggaat catcccacca gccattacag ctgccatcaa
     7621 gctgccatcg ccagtatccg tgaagatacc gattgagaag gcataagctg ctttgttgaa
     7681 aggaccaccc atatcgattg ccatcatgcc gccaagaaca gcacccaaca gtactgcgtt
     7741 acccgtaccc aagttttcca ggaaagtgat catagctgta ttgattgtgg agaagacagg
     7801 acctaccaag aagtacatca acaagccagt gatcaataga ccgaacacag ggtacaagag
     7861 aattgtcttc atgccatcca aggtacgagg caatccttta aaggctttct tcagataaac
     7921 catgatgtaa ccggccaaga aaccagcaat caatccgccc aagaatccag catttgcttg
     7981 cacagccaag taaccgcccg ccatacctgg gagtaacccc gga
//
LOCUS       DAOMUJ010000024         7914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2388_length_7914_cov_2.323196, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000024 DAOMUJ010000000
VERSION     DAOMUJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_7914_cov_2.323196"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="truA"
                     /locus_tag="PLT73_01260"
     CDS             complement(<1..557)
                     /gene="truA"
                     /locus_tag="PLT73_01260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM19042.1"
                     /translation="MRNIKMTIEYDGGRYLGWQRLGDSDKTIQGKIENILSAMTGAEI
                     EIIGSGRTDAGTHARGQVANFKTTSKMDLTAMQDHLIRYLPRDIVVKELEEVPERFHA
                     RYHAAGKTYSYHVWNNAVPSAFERHYSYHYPGQLDVDRMNEACQKLVGKHDFLGFSSL
                     KKSKKSTVRTINDITIQQEGNLLHFS"
     gene            891..2243
                     /gene="trkA"
                     /locus_tag="PLT73_01265"
     CDS             891..2243
                     /gene="trkA"
                     /locus_tag="PLT73_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRM19043.1"
                     /translation="MKIVIAGGGKVGSVLCAELATQENDIILIEKMEMRLDKLISTYD
                     ISGIVGNGASYDIQQEAGVADCDIFIAVTAQDEVNIMASILAKKLGAKYTIARVRNPE
                     YSGHFDYVRESLGINLMINPELESARSIARIIKFPTVLSIQSFADNRVELIEIEIGST
                     VSLADLSLADFRKKFGDVLVCAIRRKNETLIPSGQDRLLVGDHAYIIGNRATIRKFYR
                     TVCPQKTKINSALVIGGGRLTYYLLDFLKEYRMDIKVIEQSRQTAEELSAAYPNVEVI
                     LGDGTDQDVLQQEGIETYNAFISLIGVDEENIIASLYAIQQNVEKVITKVNRELLLKI
                     FDSLGMETIITPKRVIANTILRFVRSLGNSSVSDVEALYRLADNEAEAIQFKVKETSR
                     IIHIPLVRLNTKPNLLIAYIIRNKKLIIPSGQDVIKPNDRVIVVTKEKNYDDIDDIVY
                     "
     gene            2259..3704
                     /locus_tag="PLT73_01270"
     CDS             2259..3704
                     /locus_tag="PLT73_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM19044.1"
                     /translation="MNVSIVRYVVARLLQIEAALMIFPLAVSLLYRESWNYLFSFLSV
                     MIFLAVLGQLLSLKKPENKRMTAREGFVIVAISWILFSFFGSLPFVINSDIPSFVDAF
                     FETSSGFTTTGASILDDVESLSHSSLFWRSFTHLVGGMGILVFALAVLPQIDSQSVNI
                     MKAETPGPTFGKLVSKLSSSARILYLIYLSMTAIVTILLWIGGMDLFDSLLHAFGVAG
                     TGGFGIKNGSITPYENAYVEWVIGIGMLAFGINFNMIYLLLIGNIKQVFKSEELKWYF
                     LIVLGAVGLICINLIGYYDSISKLIRDVFFTVSSVITTTGFTTADFGSWPLFSQMVIL
                     LLMFFGACAGSTAGGLKISRVVVYVKILVAEIKRVAQPNRIVVVQYDQKPLGTNTKHG
                     VANYLIIYSVVFAAVFMLISLDAPDFLSAFSAVAATFNNIGPALGAVGPAYSYSKLHD
                     YSKILLSFTMLAGRLEIFPILILFAPRTWKK"
     gene            complement(3819..4151)
                     /locus_tag="PLT73_01275"
     CDS             complement(3819..4151)
                     /locus_tag="PLT73_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HRM19045.1"
                     /translation="MSNQPTFTEVKEALFPTLEQYVPIVARVHGKNHPEFHDVHQLFN
                     AILEKTLTADTKKPDLNEEFIKLRAVSNNYTVPDDVCESYEAVYQMLAKVDQAYQASL
                     SPRSSRSI"
     gene            4318..>5444
                     /locus_tag="PLT73_01280"
     CDS             4318..>5444
                     /locus_tag="PLT73_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRM19046.1"
                     /translation="MKSKGEEVDYKTFVAMIEEGSVESVLVEDSRIEFTALNDEGEEA
                     VFITGRMEDPALTERLLATNAKFTQEIIPEESPLLNFFLIWILPMVLLYALWNFMMRR
                     LQGRVGGLGGSEMKFGKSNVMVYAASETGKTFADVAGQDEAKEALWEVIDFLHNPQKY
                     REIGAKMPKGILLVGPPGTGKTLLAKAVAGEAKVPLFSISGSEFMEMFVGVGAARVRD
                     LFNQAQAKAPCIVFIDEIDTIGKSRNLSGITGNDERDQTLNQLLNEMDGFSSEKGVVI
                     LAATNKPEVLDKALLRPGRFDRRIPLELPDIAGREAILRVHAKTVKMTEDVDLKGIAK
                     YTPGSSGAGLANLINEGALHAIKEERSKINQSDLEYALEII"
     assembly_gap    5445..5454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5455..6065
                     /locus_tag="PLT73_01285"
     CDS             <5455..6065
                     /locus_tag="PLT73_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRM19047.1"
                     /translation="GYQRKNAILSKEDKLAIAYHEIGHAIVAAKQTDSAPVNKISIIP
                     RTSGVLGYTMQSEETDQILMSREKFLNKIATYMGGRSAEEVILNTVTSGAENDIEAAT
                     AIARAMVTRYGMSEKFDMMALETINNPYLGTDVTSLVSNETSAKIDDEVLRIITFAHE
                     KAKEIIRENVPKMHELANYLLEKETMTGEEFMEMLEEKDDGG"
     gene            6159..6509
                     /locus_tag="PLT73_01290"
     CDS             6159..6509
                     /locus_tag="PLT73_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRM19048.1"
                     /translation="MKNTKIFGMSFASVYPLYIKKAEKKGRTKEEVDEIIFWLTGYDE
                     ATLQQQLENKVDFETFFAQAPQLNPNVSLITGVICGYRVEEIEDKLMQKIRYLDKLID
                     ELAKGKAMEKILRK"
     gene            6561..6875
                     /locus_tag="PLT73_01295"
     CDS             6561..6875
                     /locus_tag="PLT73_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRM19049.1"
                     /translation="MEFKNVTVIKEANVYFDGKVTSRTVLFESGEKKTLGFMLAGEYE
                     FNTDAEEIMEVLGGDMSVMLPGEAEYTLYSKGQSYVVPANSSFKMIVEKYVDYCCSYK
                     ND"
     gene            complement(6990..7331)
                     /locus_tag="PLT73_01300"
     CDS             complement(6990..7331)
                     /locus_tag="PLT73_01300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002385138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM19050.1"
                     /translation="MNASDRYIPKKGDIVWVNFDPSSGNEIKKRRPGLVVSRYEFNRS
                     TLFAVICPITSTIKNLPTRYTLPEEMVVTGQIVISQLKSLDFHARKIEFADRLPVQDI
                     ANVDQIIEYIF"
     gene            complement(7331..7561)
                     /locus_tag="PLT73_01305"
     CDS             complement(7331..7561)
                     /locus_tag="PLT73_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010775692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRM19051.1"
                     /translation="MLITKSRLQGSSVVVTLPLHNGEKLEANKEYVVIYSPDGTITLV
                     PKIDDPFVLSEAGAFYEKDEWNNLSPEGRELF"
BASE COUNT         2211 a         1597 c         1928 g         2168 t
ORIGIN      
        1 gaaaagtgca gcagattgcc ttcctgctgg attgtgatgt cattgatggt ccggaccgtc
       61 gattttttcg atttcttgag tgaagagaac ccgaggaaat catgtttccc gacaagcttt
      121 tggcaagcct cgttcattct gtcaacatca agctggccag ggtaatggta gctgtaatgg
      181 cgctcaaatg cggaaggtac ggcattgttc cagacatgat agctgtatgt ctttccggct
      241 gcatgatagc gggcgtggaa cctttccgga acctcctcca gctctttgac gacaatatcg
      301 cgcggaagat aacggatcag atggtcctgc atcgctgtca aatccatttt ggatgtcgtc
      361 ttgaaattag ctacttgtcc ccgtgcgtgg gtgccggcat ctgtccggcc tgagccgatg
      421 atttcaatct ccgcccccgt cattgccgac aggatgttct ctattttgcc ttgaatggtt
      481 ttgtccgaat ccccgagcct ttgccagcct aggtatctgc cgccatcata ctcgattgtc
      541 atttttatgt ttctcatttt atctccctta actatcggtt tctgggcatc gttgcctgcc
      601 gtgtactcct gtattatacc gaatatgatt ccgatttggg atgttttcgt gtaatcttta
      661 taaacagtat ctcagtcttc tttttccatt cattcataac gtccaagcaa ccataggcaa
      721 tttgtgcact tcagctaaat ctatccgatt tttcttatga actaaatgcc aaaatgttgg
      781 atacttatcc agattcatct attagtatct tgtgtatcca cacgaaatgt gcgaagatac
      841 actcgttatg tcataatgga agagaagtca gaaacgaaag gacgttttga atgaaaatcg
      901 tgattgccgg tggcgggaag gttggttccg tgctctgtgc agaattggct acacaagaaa
      961 atgatattat tttgatcgaa aaaatggaaa tgcgtttgga taagctgatc agcacgtatg
     1021 atatctcagg catagtcgga aatggtgcca gctatgatat ccaacaggaa gcaggtgtcg
     1081 ctgactgcga catttttatt gcagtgacag cacaagatga agtgaatatc atggccagca
     1141 tcctggcgaa aaaactcggg gcaaaatata cgattgcacg cgtacgcaac ccggaatatt
     1201 ctgggcattt tgactatgtc cgtgaaagct tgggcatcaa tctgatgatc aatcccgaat
     1261 tggagtcggc gcggagcatt gcgcgtatca tcaagttccc aacagttctg agcatccaat
     1321 cttttgcgga taaccgcgtt gagttgatcg aaatcgaaat tgggtcgact gtttcccttg
     1381 cagacttatc gctagccgat ttcaggaaaa aattcggtga cgtactggtg tgtgctatcc
     1441 gccggaagaa tgaaacactg attccctctg gtcaggacag gctacttgtt ggggaccatg
     1501 cctatatcat aggaaacagg gcaacaatcc gtaaatttta tagaacggtc tgtccccaaa
     1561 aaacaaaaat aaactccgct ctcgtgatcg gtggaggaag gctgacctat tacttgcttg
     1621 atttcctgaa agaataccgg atggatatca aagtcatcga acagagccgg cagaccgctg
     1681 aggagctgag cgcagcctat ccaaatgttg aagtaatact gggtgacggg acggaccaag
     1741 atgttcttca acaagaaggt atcgaaacgt acaacgcctt catttctttg attggggtag
     1801 atgaagaaaa tattatcgct tcattatatg ctatccaaca aaatgtggaa aaagtgatca
     1861 ccaaagtgaa tcgggaatta ttgctgaaaa tatttgattc actaggaatg gaaacgatca
     1921 ttacgccaaa acgtgtgatt gccaatacga ttttacgctt tgttcgtagt ctgggaaatt
     1981 cctcggtgtc cgatgtcgaa gctttgtatc gtctggcgga caatgaagcg gaagcgatcc
     2041 agttcaaagt gaaagaaacg agccggatca tccatatccc gttagtaaga ttgaatacaa
     2101 aacccaatct gctgatcgca tatattatcc gcaataaaaa actgatcatc ccctccggac
     2161 aagatgtcat taagccgaat gatcgcgtta tcgttgtcac gaaagaaaaa aattatgatg
     2221 atatcgacga catcgtttat taggaagggg aatgcggaat gaatgtatcg atcgttcgtt
     2281 atgttgtggc acgtctgttg cagattgaag ccgcattgat gattttccct cttgcggtga
     2341 gcttgcttta tcgggaatct tggaactatc ttttcagttt tttgtccgtg atgatttttt
     2401 tggcggtgct cgggcaacta ttaagcttga aaaagcctga gaataaacgg atgactgccc
     2461 gggaaggctt cgtcatcgtc gcgatttcct ggattttgtt ctcctttttc ggcagtctgc
     2521 cttttgtcat taattcggat atcccttctt ttgtggatgc atttttcgag acgagcagcg
     2581 gattcacaac aacaggtgcg agtattttgg atgatgtgga atccttatcc cattccagct
     2641 tattctggcg aagttttacc cacttggtcg gaggaatggg gattttggtt tttgccttgg
     2701 cggtcttgcc gcaaatcgat tcccaatccg ttaatatcat gaaagccgaa acacccggtc
     2761 ctactttcgg caaacttgtg tcgaaattgt cttcttcggc tcggattttg tatctgattt
     2821 acctttccat gacagccatc gtcactatcc tgctctggat tggcgggatg gatctgtttg
     2881 attctctgct tcatgctttt ggtgtagctg gtacgggagg tttcggcatc aaaaatggca
     2941 gtatcacgcc ttatgagaat gcctacgttg agtgggtaat cggtataggt atgcttgcct
     3001 tcgggataaa cttcaatatg atttatttat tgctgatagg aaatatcaag caagtattca
     3061 agagtgaaga gttgaagtgg tatttcttga ttgtgctggg tgctgtgggg ctgatttgta
     3121 tcaatctgat agggtactat gattcaattt cgaaattgat cagggatgtt tttttcacgg
     3181 tatcttctgt cattacgaca accggattta cgacagcaga ctttggttcc tggccgttat
     3241 tttcccagat ggtgattttg ttgctgatgt tttttggggc atgtgcgggt tctacggctg
     3301 gaggattgaa gatttcgcgt gtcgttgtct atgttaagat tctggttgcg gaaatcaaac
     3361 gtgtcgcgca gccgaatcgg atcgtggttg ttcaatatga tcaaaagcct ttggggacaa
     3421 atacgaagca cggtgtagct aattatctga tcatctactc ggtagtcttt gcagcagtgt
     3481 tcatgctcat cagtctggat gcgccggact ttttgtcggc tttcagtgca gtagcggcaa
     3541 cgttcaataa tatcggtcct gctttgggag cagtcggacc ggcatacagc tacagcaaat
     3601 tgcatgatta ctcaaagatt ttgttgtctt tcacgatgct tgcggggcgt ttggaaattt
     3661 ttcccatttt gatccttttt gcaccgcgta catggaaaaa atagaaataa gaggcgtgga
     3721 tagcgcggcc aaccgtgctt gatcatgaaa aattcaaaaa actgatgctg acataaagtc
     3781 gacatcagtt ttttgctgta aagtgtcccg gtaagtgctc agatagagcg ggaacttctt
     3841 ggcgataatg aagcttggta agcttgatcc actttcgcca acatctgata gaccgcttcg
     3901 taactttcgc acacatcatc cggaactgtg tagttattcg aaaccgcgcg caattttatg
     3961 aattcctcgt tcaggtctgg tttttttgtg tcggctgtta gggtcttttc gaggatcgcg
     4021 ttgaatagct ggtgaacatc atggaattcc gggtgatttt ttccatgtac acgggctaca
     4081 atcggcacgt attgttccaa tgttggaaag agagcttcct tcacttctgt aaatgtgggt
     4141 tgattggaca tctgagatcc ttctttctga gttctgatgc tttcattata atgctgatcc
     4201 gccaaaagat acttgatctg tgtcaagaaa aagaaaatcg atacgacgct ctttcgaaat
     4261 tggctggaat atcctggata gcgaattgat accgccttca tatcatttgg cggtataatg
     4321 aagtcaaagg gtgaagaagt ggactacaag acgtttgttg ccatgattga ggaagggagt
     4381 gtggaatcgg tgctggtgga ggatagccgc atcgaattca cggcactgaa tgatgaaggc
     4441 gaggaagctg tcttcataac tggtcgaatg gaagatccgg cgctaactga gagactacta
     4501 gccacgaatg cgaagtttac ccaggaaatt atcccggaag aatccccact gttgaatttc
     4561 ttcctgattt ggattctgcc catggtgttg ttgtatgcgt tgtggaactt tatgatgcgt
     4621 aggctgcagg gaagagtcgg agggctcggc ggcagcgaaa tgaaattcgg caaaagcaat
     4681 gtcatggttt atgctgcatc tgaaacagga aaaacatttg cggacgtagc aggccaggac
     4741 gaagcgaaag aagcactttg ggaagtgatc gattttctgc acaatcccca aaaataccgg
     4801 gaaatcgggg cgaagatgcc gaaagggatt ctgcttgtcg gacctccggg aaccggcaaa
     4861 acgctgcttg caaaagcggt cgcgggagaa gccaaagttc cgctcttttc gatatcgggg
     4921 tcagaattta tggagatgtt tgtcggtgtc ggagcagcca gggtgcgaga tctgttcaat
     4981 caagcccagg caaaagcccc ctgcatcgtc ttcattgatg aaatcgatac gatcggcaag
     5041 agccgtaacc tgtctggaat caccggaaat gatgagcggg accaaacgct caaccaattg
     5101 ttgaatgaaa tggacggttt ttcctcggaa aaaggcgtgg tgatcctggc ggcaacgaac
     5161 aaacctgaag ttcttgacaa agcgctgttg aggccagggc gttttgacag gcgcattcca
     5221 ctcgaactgc cggatattgc cggcagagag gcgattctga gggtgcatgc aaaaacagtc
     5281 aaaatgacgg aggatgttga tttgaaagga atcgcaaagt atacacccgg cagctccgga
     5341 gctggccttg ccaatctgat aaatgaaggc gctctccatg ccataaaaga agagcgcagc
     5401 aagatcaatc aatccgattt ggagtatgcg ttggaaatca tattnnnnnn nnnnccggat
     5461 accaacggaa aaatgcgatc ctctcgaaag aagacaaatt ggcgatagcg taccatgaaa
     5521 tcggccatgc gatcgttgcc gcaaaacaga ccgattccgc accagtcaat aaaatcagca
     5581 tcatcccaag gacatccggt gtgctcggat acacgatgca gtcggaagag acagaccaaa
     5641 tactgatgag ccgggaaaaa ttcctgaata aaatcgccac ctacatgggc ggacgctctg
     5701 ccgaggaggt catcctgaat acggtcacct ccggggcgga aaatgacata gaggccgcaa
     5761 cagcgattgc gagagcgatg gtcacacgat acgggatgag tgaaaaattc gacatgatgg
     5821 ctttggaaac gatcaataat ccttatctgg gaacggatgt aacctcactc gtatcgaatg
     5881 aaacctctgc gaaaatcgat gatgaggttc tgcgcatcat cacattcgcc cacgaaaaag
     5941 ccaaagaaat catccgggag aacgtaccga aaatgcatga gcttgcaaac tacctgctcg
     6001 aaaaagaaac catgaccggc gaggaattca tggaaatgct agaggaaaaa gatgatggag
     6061 gctaatgaat cgagttttta catttttttt cgatttgtgg cataataaac atcataagta
     6121 tggaaaatag attcaggaag tttcagaaag gggtcagtat gaaaaatacg aaaatatttg
     6181 gaatgtcgtt tgccagcgtt tatccgttgt atatcaagaa agccgagaaa aagggccgca
     6241 cgaaagaaga agtcgatgaa atcattttct ggctgaccgg atatgatgag gcaactctgc
     6301 agcaacagtt ggaaaacaaa gttgatttcg aaactttttt tgcacaggca ccccagttga
     6361 atcccaacgt ttcgcttatc acaggagtca tctgcggtta ccgcgttgaa gagatcgaag
     6421 acaaacttat gcaaaagatc cgttatctgg ataagttgat cgacgaattg gcaaaaggca
     6481 aggccatgga gaaaatacta agaaaatagg ctatacgtgc aagcaaacca taaaataaac
     6541 aggctaatgg aggaaacagg atggaattca aaaatgttac agtgatcaag gaagcaaatg
     6601 tgtattttga tgggaaagtt accagcagaa cggttttgtt cgaaagcgga gagaaaaaga
     6661 cacttggctt catgttggca ggcgagtacg aattcaacac tgatgcagag gaaataatgg
     6721 aagtcctggg tggcgatatg tcggttatgt tgccgggtga agcggaatac acgctttaca
     6781 gcaaaggcca aagctatgtt gttccggcaa actcatcctt caagatgatt gtcgagaaat
     6841 atgtggacta ttgctgttca tacaaaaatg actaaatctg aaacaccacg tatacagtaa
     6901 ccgtctggca atacagccaa accaactgat tttaaaaaaa taaaagaata catgatgaaa
     6961 atccgcaatc atttgtttga ttgcggattt taaaatatat actcgattat ctgatcgacg
     7021 ttagcgatgt cctgaacagg caatctatca gcaaattcga tttttctggc gtgaaaatcc
     7081 aaagatttga gttgggagat gacgatttga cctgtgacaa ccatttcttc cggtaacgta
     7141 taccgagtag ggaggttttt tattgtcgaa gtgatggggc aaataaccgc aaacaaggtg
     7201 gaacgattga attcgtatct tgagacaacc agtcccggcc gacgtttctt gatttcattt
     7261 cctgaagatg ggtcaaaatt tacccagacg atatcgcctt tcttggggat gtacctatcg
     7321 gaagcgttca tcagaatagt tctctccctt caggagataa attattccac tcatcttttt
     7381 cgtaaaaagc tccagcttcg gacaaaacaa agggatcatc aatttttgga accaatgtga
     7441 ttgtcccatc tggtgaatag attacgacat attctttatt ggcttctaat ttttcgccat
     7501 tgtgaagcgg cagggtgaca actacggaac ttccttgcag tctggacttt gtaattagca
     7561 tgtttctcac ctcttatgaa taattatact cgacaacact cagtcacgca aagtgcacta
     7621 tcggcacatg ctaaaaaagt aaatttagga atataatcga tttatggagt cgatgaccct
     7681 agggaagtca gccgacagaa caatggaatt caggagggat acggtgatgg atatgacgaa
     7741 ttatgccatc atgaccatca tcgaactcga gaataaaaca taatgttgcg tacaatagta
     7801 tccagtgcct caaaaaagat agacgatacc agagcatcca agctctggta tcgtctatct
     7861 ttttaaatcg tgatgctgtt gatgaagcgg gcgatccttg gatcggttgt gctt
//
LOCUS       DAOMUJ010000025         7690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2494_length_7690_cov_2.372233, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000025 DAOMUJ010000000
VERSION     DAOMUJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_7690_cov_2.372233"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..532
                     /locus_tag="PLT73_01310"
     CDS             155..532
                     /locus_tag="PLT73_01310"
                     /inference="COORDINATES: protein motif:HMM:NF023305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HRM19052.1"
                     /translation="MQNFHYFEILLYVFPVLEIILINQFFRPYLRYKQIIRLSIADVV
                     LPFLLVGIHLLSVYSLTYSLLPHFLLAACIVGLVQTLYFDKRKKNHEPKRFYRYFIKI
                     LFLMALLFYYSLVLLRVYFLIRG"
     gene            566..1609
                     /locus_tag="PLT73_01315"
     CDS             566..1609
                     /locus_tag="PLT73_01315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM19053.1"
                     /translation="MKVLLYSEGMKYIGKSGLGRAIEHQKKALTLEGISFTTDYRDDF
                     DLMHINTYFGQSYRLLHKVKKQGKPIVYHAHSTKEDFQNSFIFSNAAAPLFSKWILHC
                     YSHGDVVLTPTPYSKQLLQQAGLEQPIIPISNGIDLSFYKATAEMGASFRQKYGYKET
                     DKVIMSVGLYIKRKGILDFVALAKAMPAYQFIWFGYLNLHQVPHEIREAVETKLPNLQ
                     FAGYVAPDELRNAYCGADLFFFPTYEETEGIVLLEALAMGQEILIRDIPIYEGDLVDG
                     KHVYKGKTNDDFQRLINGILEGDLPSLKAAGQERIRQKDLRCIGSELRSAYETAIQLH
                     KSDGGGMSVPGTK"
     gene            1753..2184
                     /gene="mraZ"
                     /locus_tag="PLT73_01320"
     CDS             1753..2184
                     /gene="mraZ"
                     /locus_tag="PLT73_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRM19054.1"
                     /translation="MLMGEFKHNIDAKGRLIMPAKFREALGDTFIVTRGLDGCLFGYP
                     LSQWELLQEKLKQLPLAKKDARAFTRFFYSAAMEAELDKQGRINIPQTLLDYAKIEKE
                     CRIVGVADRIEIWSNEKWEAFADEAAENFEDIAETMIDFGL"
     gene            2193..3143
                     /gene="rsmH"
                     /locus_tag="PLT73_01325"
     CDS             2193..3143
                     /gene="rsmH"
                     /locus_tag="PLT73_01325"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM19055.1"
                     /translation="MEKFEHYSVLLKESIDGLNIKPDGIYVDCTLGGAGHSSVILSKL
                     NENGHLYAFDQDRIAIENAEAMLATYIEKGMVTLIKANFRNIKEELETLGVYGVDGIL
                     YDLGVSSPQLDQAERGFSYRYDAPLDMRMDQEQELTARVIVNEWPFGELVKIFYRYGE
                     EKFSKQIARKIEKIRETQPIETTGELVEIIKDCIPAPARRKGGHPAKRIFQALRIAVN
                     DELSAIEDSIEDGLKMLNVGGRMSVISFQSLEDRIVKVLFKEASSKEDTLPLLPILPE
                     EYEADYKLISRKPILPNEAELSENSRSQSAKLRIIERIKK"
     gene            3239..3619
                     /locus_tag="PLT73_01330"
     CDS             3239..3619
                     /locus_tag="PLT73_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRM19056.1"
                     /translation="MALPEYRAAIAEPKLPKELTEPRKGQPDPKGYRAVRVKYIKIEK
                     FAITITLLSALIVLLLSLATQVTLSNQNRALQDLQNDSVAISLENQNLEQEVQELSRY
                     DRIIEIAKELGLEMNEANVRNVTR"
     gene            3616..5775
                     /locus_tag="PLT73_01335"
     CDS             3616..5775
                     /locus_tag="PLT73_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRM19057.1"
                     /translation="MKKTANPQKNRKKMTRIMVGLTGLLFLVFIFRFSMIMITKKVNG
                     ENLSEHVNNLYTRSSILAAKRGTIYDIGGNPIALDATSYSLVAVLTDEWSEDTENPNH
                     IVDKEKTAEVLAQYISMSEGEILTILNQADLKQVEFGSAGTNLNYATKSSIEAENLPG
                     ITFEETPSRLYPNGIFASHLVGFAQSSASEEEADTNEEKKLVGMMGIESLYDDDLDGS
                     DGEVTYQEDSNGYLIPGTQIQEKEPVEGDNLYLTLDSRLQIYLESLMSQVYAEANPES
                     MTAMLVDPETGAILAASQRPTFNATTKEGIDAQWQNLLVEDTYEPGSTFKILTLAAAV
                     NEGVFSPFATYTSGSVEVEGGTIRDYNKDGWGNITYLEGLARSSNVAFVKLVQAMGYD
                     VWESYMKAFGIGQSTDSGLENEATGNYSYTYPLEKANTAFGQGVTVTPFQLMQAFTSI
                     ANEGTMMKLNYLDRTEDPNTGEVTAIEPEEVGSPITAETAQTVLTYLKEVVYNENGSG
                     QAYQIEGTEIAAKTGTAEVVNSTTGGYYTGYSNYLYSVVGFAPADDPSYILYLTMKRP
                     ANLDSLNGSKYLSEIFNPFMTRAIEYDNLNTEITGDVNQASMPEVTNLSKDEALAALT
                     AVGYNDTAIIGSGSRIVQQYPYAGTQTITNQKTILMTEGAMTMPDVYGWSKDDVLKVA
                     EIAGINFTFEGEGYVVFQSLETGSMLNAATEVQIILE"
     gene            5841..6797
                     /gene="mraY"
                     /locus_tag="PLT73_01340"
     CDS             5841..6797
                     /gene="mraY"
                     /locus_tag="PLT73_01340"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010013746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM19058.1"
                     /translation="MHWTEMLLPLSISFAITISMMPIFIGYFKVKQFGQITREEGPKW
                     HQVKSGTPTVVFIIAATLTVIGTAIWKDVLTVKLAMLLFVLLFFAAIGFLDDFLKIFK
                     KQNEGLTSKQKFLSQLVGSFVFVALFFLSGSDPLITIPFFGQIHNWLVFAVFTIIWIT
                     GFSNAVNLTDGLDGLVAGTAGIAYTAYGILAYRQGQLEVLLFCIAIVGGLIGFFFFNK
                     KPAKIFMGDVGSLALGAGLAVVSLALHLEWSLLLIGLVFVIETASVMLQVGSFKLRGK
                     RIFKMSPIHHHFEMSGWSEWRVVLTFWAVGLVSAILALWLLV"
     gene            6816..>7690
                     /gene="murD"
                     /locus_tag="PLT73_01345"
     CDS             6816..>7690
                     /gene="murD"
                     /locus_tag="PLT73_01345"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRM19059.1"
                     /translation="MRTNNRFENKKVLVLGLALSGMNAAKLLLELGAMVTVNDAKELN
                     DNPDAKELISSGIKVIAGSHPIELLDESFSFMVKNPGIPYNNPMVKRALELGIPVLTE
                     VELAAEILEGRLIGVTGTNGKTTTTTMITDLLNAGRKIGKAYKAGNIGVPASAVARVA
                     TADDDVVMELSSFQLMGIEAMRPAIAVITNITEAHLDYHGDRKEYVKAKWRITENQTA
                     DDYLVLNWDQEELREMSQLSKAQIVPFSRTQVLEDGAYASEGSLYFKGEKVMAISDLR
                     VPGEHNIENALAAIAV"
BASE COUNT         2191 a         1619 c         1909 g         1971 t
ORIGIN      
        1 ctaatcatga aataccatcc gcatttccag tcagcagaat gaattttctg ttggcttttt
       61 ttgttcccat ttttcaagga ggcataattt gcttggatga ggtaaaatat atgcatgcgg
      121 taagaattcc atattttagc aaaggagcgt cgggatgcaa aacttccact attttgaaat
      181 ccttttgtat gtttttccgg ttttagaaat cattctgata aatcaatttt ttcgaccgta
      241 tctcaggtac aaacaaatta tccgattgtc gattgcggac gtcgtgttgc cgtttttgct
      301 ggtgggcatc cacctgttga gtgtctacag cctgacttat tcgctgctgc ctcatttttt
      361 actggctgcc tgcatcgtcg gtctggtgca gacgctgtat ttcgacaaac gtaaaaaaaa
      421 ccatgagccg aagcggtttt atcgttattt cattaaaata ctatttttga tggcactgct
      481 tttttattac tctttagtgc tgttgcgcgt ttatttcctt ataagaggat agtccgaaaa
      541 ttgaagaacg aggtgatacg aaagcatgaa agtattattg tactctgaag gcatgaaata
      601 catcgggaaa tccggtttag gcagagcgat cgagcatcag aaaaaagctc tcacactgga
      661 agggatctct tttacgacag attacagaga tgattttgat ctgatgcaca tcaataccta
      721 cttcgggcaa tcttatcgtc tgctgcataa agtcaaaaag caggggaaac cgatcgttta
      781 tcatgcccac tccacaaagg aggatttcca gaattccttt attttctcca atgctgctgc
      841 accgttgttc agcaagtgga ttctgcactg ctacagtcat ggcgacgtgg tcttgacgcc
      901 tactccatat tccaagcaat tgctgcagca agcaggttta gaacaaccga tcataccgat
      961 ttcgaacgga attgatttgt cgttttacaa agcgactgcg gagatggggg cgtcttttcg
     1021 gcagaagtac gggtacaagg aaacggacaa agtcatcatg tctgtcggcc tttacatcaa
     1081 acgcaaaggg attcttgatt ttgtcgcctt ggccaaagcg atgcctgctt atcagtttat
     1141 ctggttcgga tacttaaacc ttcaccaagt tccgcatgaa attcgggaag ctgtggaaac
     1201 aaaattaccc aatctgcaat ttgccggtta tgtggcccct gatgaactca gaaatgcata
     1261 ctgtggtgcg gacttgtttt tcttcccaac ttacgaagaa acggaaggaa tcgttcttct
     1321 ggaggcgctc gctatgggac aggagatcct gatccgggat ataccgatct atgagggtga
     1381 tttggtcgat ggcaaacatg tctacaaagg taagacgaat gacgatttcc aaaggctgat
     1441 caacggcatc ctggaagggg atttgccttc tctgaaagcc gcaggacagg agcgtatccg
     1501 tcagaaggat ctccgttgca ttggttctga attgaggagt gcctacgaaa cagccatcca
     1561 gctgcataag tcggatggtg ggggaatgtc ggttccaggg acaaaatgat agatgacaac
     1621 taaaaacaaa ttgatttcga aaaataatcg aaatcaattt gtttttttta tgaattgttc
     1681 gggaaaatgt ggtagaaagt ggggcgatgt ggtagactga aaacatattt gaaggtgggg
     1741 gcgagtgtct ttatgttgat gggcgaattc aaacacaata tagacgccaa aggcagattg
     1801 atcatgcccg caaaatttcg tgaggcatta ggcgatacat tcatcgtcac ccgtggattg
     1861 gacggctgtt tgttcggcta tccgttaagc caatgggagc tgctccaaga gaaattgaaa
     1921 caactgcccc ttgccaaaaa agatgcgcgt gcttttaccc gtttcttcta ttcagccgct
     1981 atggaagctg aattggataa acaagggcgc atcaatatcc ctcaaacatt attggattat
     2041 gcgaaaattg aaaaagaatg tcgtattgtc ggagtagccg accgtattga aatatggagc
     2101 aacgagaagt gggaagcatt tgctgatgaa gctgccgaaa acttcgagga tattgctgaa
     2161 acgatgatcg actttggatt gtaggtgtaa aaatggaaaa atttgaacat tatagcgtgt
     2221 tgttaaaaga gtccatcgat ggtttgaaca tcaaaccaga tggcatttat gtcgattgca
     2281 ctttaggtgg tgccggacac agcagtgtga ttttatcaaa actaaacgaa aatgggcatc
     2341 tgtatgcctt tgaccaagac cggatcgcta tcgaaaatgc tgaagcgatg ttggcgactt
     2401 atatcgaaaa aggcatggtg acactcatca aggctaattt tcggaatatc aaagaagaac
     2461 tggaaaccct tggtgtttat ggcgtagacg gcatcctgta cgatttggga gtgtcttccc
     2521 cgcaattgga ccaagctgaa agagggttca gttaccgcta tgatgcccca ctcgatatgc
     2581 gtatggacca agaacaggaa ttgacggccc gcgtcattgt gaatgagtgg ccttttggtg
     2641 aattagtgaa gattttttat cgttatggcg aagaaaaatt ctcaaaacag attgcccgca
     2701 aaatagaaaa aatcagagaa acccaaccga tcgagacgac cggcgaattg gtcgaaatca
     2761 tcaaagactg catccctgcg cctgccagaa gaaagggtgg gcacccggcc aaacgcattt
     2821 ttcaagcttt gcgaatcgcc gtcaatgatg aattgtccgc tattgaagat tcgatagaag
     2881 atggcttgaa aatgttgaac gtcggaggac gcatgagcgt catttccttc caatccttgg
     2941 aggaccggat cgttaaagtc cttttcaaag aagccagctc gaaagaggat acattacctt
     3001 tgctgcctat tcttcccgag gaatatgaag cggactacaa gctgatcagt cgaaaaccaa
     3061 tcctgcccaa tgaagcggaa ttgagtgaaa attcacgctc ccaaagtgca aaattgcgta
     3121 tcatcgaaag aatcaagaag taacaggagc gcatcagctc tgtaacgaaa gatcatcagt
     3181 cggaaccagt caattgtgca gtagctgtat aattgactgc gggggaggag aggattaagt
     3241 ggcattacct gaatataggg cggcgattgc agaaccgaag ctgccaaaag aattgacaga
     3301 acctagaaaa ggccagcctg atccgaaagg ctatagagcc gttagagtta aatatataaa
     3361 aattgaaaaa tttgccatca ccatcacttt gttaagtgct ttgatcgtat tattgttgtc
     3421 tctggctaca caagtgaccc tctccaatca gaatagagcg ttgcaggact tacaaaatga
     3481 tagcgttgcg atcagtctgg aaaatcaaaa tttggagcag gaagtccaag aactttcaag
     3541 atatgaccgc atcattgaga tagcgaaaga acttggtctg gaaatgaatg aagcaaacgt
     3601 taggaatgtt acgcgatgaa aaaaacagct aatcctcaaa aaaaccgaaa aaaaatgact
     3661 cgtatcatgg ttgggttgac aggtttacta tttcttgtat ttatttttcg tttttctatg
     3721 atcatgatca ccaagaaggt aaatggcgag aatctaagcg agcatgttaa taacttatat
     3781 accagaagta gtatccttgc cgcaaaaaga ggtaccatct atgacatagg tggtaatcct
     3841 attgccttgg atgctacttc ttattcgttg gtggcggttt tgaccgatga atggagtgag
     3901 gatacggaaa atccgaacca tatcgtcgac aaggaaaaaa cagctgaagt tctggcacaa
     3961 tacatcagca tgagcgaagg cgagattctg actatcctga accaagccga tctgaagcag
     4021 gtcgaattcg gaagcgcagg gactaacctg aactatgcta caaaatccag cattgaagcg
     4081 gaaaaccttc cggggatcac gttcgaggaa acaccttcca ggctttatcc gaatgggatt
     4141 tttgcttccc atcttgtcgg ctttgcgcaa agttcagcca gcgaggaaga agcagataca
     4201 aacgaagaaa agaaactggt cgggatgatg gggatcgaat ccctgtacga tgatgatttg
     4261 gacggcagtg atggtgaagt cacctatcaa gaagactcca atggctacct gattccaggc
     4321 actcagattc aggaaaagga acctgttgaa ggcgataatc tttatctgac gctggacagt
     4381 cgcttgcaga tctatctgga aagcctgatg tcgcaagtgt atgcagaagc caatccggag
     4441 tcgatgacag ctatgttggt cgatccggaa accggcgcca tccttgcggc ttctcaacga
     4501 cccaccttca acgctaccac caaagaaggc atagatgcgc agtggcagaa tctgttggtc
     4561 gaggatacct atgaaccggg ttccactttc aaaattttga ctttggcggc agctgtgaac
     4621 gagggcgtat tcagtccttt tgcgacctat acttccggaa gcgtggaagt ggaaggcggc
     4681 accatccgtg attacaataa ggacggttgg ggcaatatca cctatcttga aggattagcc
     4741 cgctcgagta acgtggcttt cgtaaagctg gtgcaagcga tgggttacga cgtttgggaa
     4801 tcctatatga aagctttcgg catcggccaa tcgaccgatt cgggacttga aaatgaagca
     4861 acggggaatt atagctatac gtatcctttg gaaaaggcga atacggcatt cggacaaggg
     4921 gtaaccgtta cgcctttcca attgatgcag gcttttacgt caatcgcaaa tgaaggcacc
     4981 atgatgaaac tgaactacct ggatcgcaca gaagatccga atacgggtga ggtcaccgct
     5041 atcgaacctg aagaagtggg atctccgatc acagcggaaa ccgcccagac ggtgctgacc
     5101 tatctgaaag aagtcgtcta caacgagaat ggatccggtc aagcgtacca gatcgagggt
     5161 accgagatcg ctgccaaaac cggtacggcg gaagtggtga attccacaac aggcggatat
     5221 tacactggct attccaatta tctttactcg gtagtcggat tcgcgccggc ggatgatccc
     5281 agctacatcc tgtatttgac aatgaaacgg cctgcaaact tggacagtct gaatggctca
     5341 aagtatctgt cggaaatatt caacccattc atgacaaggg caatcgaata tgacaacctg
     5401 aatacagaaa tcaccgggga cgtcaatcaa gcctcgatgc cggaagtgac aaatttgtcc
     5461 aaagatgagg ctttggcggc cctgactgct gtcggctaca atgatacagc aatcatcggc
     5521 agcggtagcc ggatcgtcca acaataccca tacgccggga cacagacaat cacgaaccag
     5581 aagacgatcc tcatgacgga aggtgcgatg acgatgcctg acgtctacgg gtggtcaaaa
     5641 gatgatgtcc tgaaagtagc agaaatcgca ggcatcaact tcacttttga aggcgaaggc
     5701 tatgtcgtat tccagagtct ggagacgggt tccatgctga acgctgctac tgaagtccag
     5761 atcattctgg agtaagcgtt cagaagcaac actcacaaaa tcaatcaaaa aaagttaagc
     5821 atctattagg aggaaatcat atgcattgga cagaaatgct gttgccttta tcgatcagct
     5881 ttgccattac aatcagcatg atgccaattt tcatcggcta ttttaaagtg aaacaattcg
     5941 gacagatcac aagggaagaa ggcccaaaat ggcatcaagt caaaagcggc acaccgacgg
     6001 tcgtcttcat catcgctgcg acccttaccg ttatcgggac agcaatctgg aaggatgtcc
     6061 tcaccgtaaa actggctatg ttgctttttg tgctgttatt ttttgcagct attggttttt
     6121 tggatgattt cctgaaaata ttcaaaaaac aaaacgaagg actgacctcc aagcagaagt
     6181 tcctttcgca attggtggga tccttcgtct ttgttgcgct gttcttcctg tcgggttcgg
     6241 atcctttgat cacaatccca ttctttggac agatccacaa ctggttggtg ttcgctgtat
     6301 ttacgatcat ctggatcacg ggtttttcga acgctgtgaa tctgaccgat ggattggacg
     6361 gcctggttgc cggtacagca ggcatcgcct ataccgcgta tggcatattg gcataccgcc
     6421 aaggacaatt ggaagtattg ctgttttgca ttgcaatcgt gggtggcctg atcggtttct
     6481 tctttttcaa taaaaaacca gctaagatct ttatgggcga cgtcggctcg ttggcattgg
     6541 gtgcaggact tgccgtcgtt tcgctggcgt tgcatctgga atggtctttg ttgctgatcg
     6601 gccttgtttt cgtgatcgaa acggccagcg tcatgctaca ggtaggatcc ttcaaattgc
     6661 gcggcaaacg catcttcaag atgtctccga tccatcatca tttcgaaatg agcggatgga
     6721 gcgaatggcg cgtcgttctg actttctggg ctgttggtct ggtttcggca attttagcct
     6781 tgtggctgct cgtctaagtg aagggagata aatacatgag aacaaataat cgattcgaaa
     6841 ataaaaaagt gttggtgctg ggcttggcgc tcagcggtat gaatgctgcc aagctgctcc
     6901 tggaacttgg cgcaatggtc acagtcaatg acgcaaagga attgaacgac aatccggatg
     6961 caaaggagtt gatttcatcc ggcatcaaag tgattgctgg atcacatccg atcgagttgt
     7021 tggatgaatc tttctcgttc atggtcaaga atccgggcat cccttacaac aatccgatgg
     7081 ttaaacgtgc tcttgaattg ggaattcctg tgctgacgga ggtcgaactg gcagcagaaa
     7141 ttctcgaagg ccgactgatc ggtgtgaccg gaacgaacgg aaaaaccact accacgacaa
     7201 tgatcacgga tcttttgaat gcaggcagaa aaatagggaa agcctacaaa gccggcaata
     7261 tcggcgtgcc tgcatcggca gtggcgcgtg tggcgactgc agacgatgat gtcgtgatgg
     7321 agctgtcgag cttccaattg atgggtatcg aagcgatgcg gcctgccatc gcagtcatca
     7381 cgaatatcac ggaagcccat ctggattatc atggggaccg caaggaatac gtcaaagcga
     7441 agtggcgcat aaccgagaat caaaccgcgg atgattatct tgttttgaat tgggatcaag
     7501 aagaactgcg tgagatgtcc caactctcaa aagcgcaaat cgttcctttt tcccgcacac
     7561 aggttctcga agatggcgcc tatgcatcag aaggttctct gtattttaag ggcgaaaaag
     7621 tgatggctat ttctgatttg agggttccag gtgagcacaa catcgaaaac gctttggccg
     7681 caatcgctgt
//
LOCUS       DAOMUJ010000026         7642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2514_length_7642_cov_3.061816, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000026 DAOMUJ010000000
VERSION     DAOMUJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_7642_cov_3.061816"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLT73_01350"
     CDS             complement(<1..940)
                     /locus_tag="PLT73_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRM19060.1"
                     /translation="MFLTPRETLILKELLHSASPVTVERLMSLLKVSKRTVYRELANL
                     ELSLESIGAKLEKESRGRFRLLAEDAAKAKILSIVSEDEPSELSATERQHAILLQLLN
                     SDEPISMQTFLDEYLISNTTFFADIKQLELRLARLPLTIARNRGYELVGSEKYRRLLL
                     ANILGIEINEYHFFHFSELEDKESFFMQFVNQEQLLFAQKLVREIVEPRFSDLSDRKL
                     EFLILMLTISMDRVPRGRQLVEESYAGQINKELLDLSKQIFAKIAAHTKQLYAVSEVV
                     FFANLLGDFFSDLDNDFFNESFDPRLAYQVKQLIENV"
     gene            complement(924..2615)
                     /locus_tag="PLT73_01355"
     CDS             complement(924..2615)
                     /locus_tag="PLT73_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM19061.1"
                     /translation="MIKPKRFYVQADLFELPKTRRVGHYLSGMIMPNVSVFITWGIIT
                     TLIQYLRGPLQSSFLEMDRLMIQFLFPVLIAYTGGRLIENRAGVVAAVAVIGMLTESE
                     DPQILGAMVIGPLIGWIVFLFDKYVLPKVEPGYEMLVRNFSAGILGMLFGYLSLVFIG
                     PIIAGITNQIGFFVGWLIQRNLLLFTNLFIEPLKVLFLNNTLNHGILTPLGIEQAGDA
                     GTSILFLIETNPGPGLGVLLAFILFSRKELKATASGAFMIHLFGGIHEIYFPFVLLNP
                     LLFAAVILGGMSGTLIFEMFQVGLKVPASPGSIVVILANAPQEMLLGVASGIAGSTLV
                     SFIIAALVIRKDQTKKEISEKVTKVTEIRTILFACDAGMGSSAMGASLMRQQLQASGI
                     SIPVDYTSIYRVNDDPHLLLITQNELKHLAEIQAPHAQLVTIGNFLDQEEYTKVITIL
                     TDEQENEMIPEAIGKKLPYQKVIFLYADGVRGSQTMAVQAFRNLAEKQNIGVDVEKQP
                     LEQLIADQHNLYIVTEAFAAENELPKGPLLVVEHLIATNKYEKLLRGDLSDVSNS"
     gene            complement(2842..4194)
                     /gene="glmM"
                     /locus_tag="PLT73_01360"
     CDS             complement(2842..4194)
                     /gene="glmM"
                     /locus_tag="PLT73_01360"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRM19062.1"
                     /translation="MGKYFGTDGVRGVANSELTPELAFKLGRFGGYVLRQHAKDIAHP
                     LVLVARDTRISGQLLEYALVSGLLSVGIEVLQLGVIPTPGVAYLTRIQGAVAGVMISA
                     SHNPAGDNGIKFFGSDGFKLSDEQEYEIEAYLDQEGDDLPRPSADGLGTVDEYPEGVL
                     KYAQYLQTTIPDDLSGITVCLDAANGAASPVVNRLFADLETDFYTMGANPDGININKG
                     VGSTHPDVLGKFVVEKKADVGLAFDGDADRCIAVDENGRIIDGDRIMFICGKYLKERN
                     KLNQNTIVSTVMSNLGFHLAVEAEGMTALKTQVGDRYVVEEMRKNGYNLGGEQSGHVV
                     FLDMNTTGDGLLTGIQLLNVMKQTGKKLSELADEVTLYPQELVNVRVSEKDGVMEIPA
                     VKAVIEEVESEMAGKGRILVRPSGTEPLLRVMAEAETDEKCHDYVMRIVNVVKAEIGI
                     "
     gene            complement(4258..5367)
                     /locus_tag="PLT73_01365"
     CDS             complement(4258..5367)
                     /locus_tag="PLT73_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRM19063.1"
                     /translation="MRKQQDDKFLENKWVVRGLSFLISLLLFTYVYSENYGWSTSTSN
                     SSISTSKRETISNVPIQVNIDTDEYFISGLPETVVLTLNGPESVLVQTISAADYRIVT
                     ENLDELGPGTHTIQLTAENLSNEIDYAITPSRVNVVIEERVAIESPVEVRFDESLVDP
                     KYLAGVPELSVDTVTLTGPASTISRVDSVYVTVAAENGLTNTVNMNTVVQVVDASGNK
                     LNVSVDPQEISVRIPISLYGKKVPLVIQQIGVPLEGKVYTIDVDGEASVTLTGDKSIL
                     ADIDEVFLPVSVTGVESNTTQTLTIPVVNDTLTATPTTIKVNIRVSNAAETPADSETT
                     ADEGESSSSADESSAVASSASDNPKSEEDQSDSSS"
     gene            complement(5364..6206)
                     /gene="cdaA"
                     /locus_tag="PLT73_01370"
     CDS             complement(5364..6206)
                     /gene="cdaA"
                     /locus_tag="PLT73_01370"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRM19064.1"
                     /translation="MSIDWGEVITWSIAFDIVDILLVWYLVYKLIMILKGTRSIQLLK
                     GIGIIVLIKLAAVTLQLQTIDWIMNRVIQWGVVAVIVVFQPEIRKGLEHLGKTGFRKK
                     AKRSINTSERLVMELDKAVQYMAKRKIGALISIEQQDLLDGYAATGIRIGGEVTNQLL
                     INIFIPNTPLHDGAVIIQDYKIATAASYLPLSESSFIPKELGTRHRAAIGLGEVSDAI
                     TIIVSEETGGVSIAHKTSLLREMSRDDFVAYLTDHLVVEEEEEKTTNFIQDFFDNTKW
                     GGSE"
     gene            6428..6850
                     /locus_tag="PLT73_01375"
     CDS             6428..6850
                     /locus_tag="PLT73_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM19065.1"
                     /translation="MSIDFKDHGKKPYVVNIEDATLQNENYRTTIWTGEKLQVTVMTI
                     QPNDDIGLEVHHGIDQFIRIEEGDGVCVMGDSEDDLYFKQKVSDDDAVFVPADKWHNI
                     TNTGDKPLKLYTIYAGPDHVPGTVHPTHEDAKNDPNEH"
     gene            complement(7186..7386)
                     /locus_tag="PLT73_01380"
     CDS             complement(7186..7386)
                     /locus_tag="PLT73_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19066.1"
                     /translation="MAKCTDCNVDLIEGLGLYSQGVYLVELAKEKTLNGHTKAALEAA
                     VCPKCGKVSFYLPEEQLAKLID"
BASE COUNT         1996 a         1969 c         1602 g         2075 t
ORIGIN      
        1 tgacgttctc gatcaattgc ttcacctggt acgccaaacg cggatcgaag ctttcgttaa
       61 aaaaatcatt atccaagtca ctgaagaaat cgcccaacag attagcgaag aaaacgactt
      121 cgctgaccgc atacaattgc ttcgtatggg ctgcgatttt agcaaaaatc tgcttcgaca
      181 gatccaaaag ttccttgttg atttgtcccg cataggactc ttccaccagt tggcggcctc
      241 gcgggacgcg atccatggaa atcgtcagca tcaaaatcaa aaactccagc ttccgatcac
      301 tcaagtcgga aaagcggggc tcgacgattt cccgcactaa tttttgagca aaaagcagct
      361 gctcctgatt gacgaattgc ataaagaaac tttctttatc ttccaattca gaaaagtgaa
      421 agaagtggta ctcattgatc tcgattccca gaatattggc caacagaagt cggcgatact
      481 tttcgctgcc taccagttca tagccgcggt ttctcgcgat cgttaagggc agacgcgcca
      541 aacgtagctc caactgtttg atatcggcaa aaaacgtggt gttgctgatc aagtattcgt
      601 ccaaaaacgt ctgcatgctg atcggctcgt cactgttcaa cagctgcagc aaaatggcat
      661 gctggcgctc cgtggcggat aattcggaag gttcatcctc agacacaatg gaaaggattt
      721 ttgcttttgc ggcatcctcc gcaagcaagc gaaagcggcc ccggctttct ttctccagct
      781 ttgcgccgat ggattccaaa gaaagctcga gatttgccaa ctcacggtag accgttcgct
      841 tgctgacttt cagcaggctc atcaagcgtt ccacggtaac cggtgaggcg gaatgcagca
      901 attcttttag aatgagggtc tctctaggag ttagaaacat ctgacaaatc tccccttaat
      961 agtttctcgt atttattcgt tgcgataaga tgttcgacga ccagcaatgg cccctttggt
     1021 agctcgttct cagcagcaaa ggcctccgtg acgatataca aattatgttg gtccgcaatc
     1081 aactgttcca atggctgctt ttcgacatca actccgatat tttgcttttc cgccagattc
     1141 cgaaacgctt gtacggccat cgtctgtgaa ccacgcacgc catccgcgta caaaaagatg
     1201 actttctggt acggcagttt ttttccaatt gcttccggaa tcatttcatt ttcttgttca
     1261 tcagtcagga tcgtgataac tttcgtgtac tcttcctggt ccaaaaaatt tccgattgta
     1321 acaagttgtg cgtgtggggc ttggatttcg gccaagtgct tcaactcatt ttgggtaatg
     1381 agcaaaagat ggggatcatc gttcacgcga taaatcgacg tgtagtccac tgggatcgaa
     1441 atcccgctcg cttgcaattg ctggcgcatc aggctggctc ccatcgcact tgaacccatt
     1501 cctgcatcgc atgcgaataa aattgtccgg atttctgtca ctttcgtcac cttttctgaa
     1561 atttcttttt ttgtctggtc tttcctgatc acgagggcag cgattataaa acttaccagt
     1621 gtgcttcctg caatgccact cgcaaccccc agtaacatct cttgcggtgc attcgccaaa
     1681 atcaccacga tcgatcccgg cgaagccgga actttcagtc ccacttgaaa catttcgaaa
     1741 atcaaagtgc cactcatccc gcccaaaatg acggctgcaa acagcagcgg attcaacaaa
     1801 acaaacggga agtaaatctc gtggatcccg ccgaacagat ggatcatgaa agcaccactg
     1861 gctgtggcct tcagttcctt tcggctgaac aggatgaacg ccaacaaaac ccccagaccc
     1921 ggtccgggat tggtctcgat caagaacaaa atcgaggtcc ccgcatcccc tgcctgttca
     1981 atccccagcg gcgtaaggat gccatggttg agcgtattgt tcaagaacaa taccttcaac
     2041 ggttctataa aaagattcgt gaacaacagc agattccgct ggatcagcca gcctacaaaa
     2101 aatccaatct gattcgtgat gcccgcaatg attgggccga taaatacaag gctcaaatag
     2161 ccgaacagca tgcctaggat tcccgccgaa aaattacgga caagcatttc ataacctggc
     2221 tctactttag gcaacacata tttgtcgaac agaaacacaa tccagccgat caacgggccg
     2281 atgaccatgg cccccagtat ttgcggatct tctgattccg tgagcattcc gatcactgcg
     2341 acagccgcca ctactccggc gcgattttcg attaatcgtc ccccggtata agcgatcagc
     2401 acagggaaga gaaactgaat catgaggcga tccatttcaa gaaaagagct ttggagcggt
     2461 ccccgaagat attggattag tgtggtaatg attccccacg tgatgaagac actcacgttc
     2521 ggcatgatca ttccgctgag ataatgaccg acccgccttg ttttcggtaa ttcgaaaaga
     2581 tctgcttgca catagaatcg ttttggtttt atcatgattg cacctaccta tcattaacct
     2641 gtcgatttcg ttacgcattg gtttcgctta cattataacc ataaacttat gattgtgtat
     2701 atttgcaatc attgttctga aggatggctt caaaccactc atgggagcta tacttcacca
     2761 ctgatcacta aacaaaaaat gccgtggaac accacctctg cagtgttcca cgaccataaa
     2821 atcgtttaca ggctctatct cttagatgcc gatttcggct tttacgacat ttacgatgcg
     2881 cataacgtag tcatggcatt tttcatccgt ttcagcttca gccataacgc gcaacaacgg
     2941 ttctgttccg cttgggcgca ccaaaatgcg tcctttgcct gccatttcgg attcaacttc
     3001 ttcgatgaca gctttcacag ccggaatctc cattacgccg tctttttcgg aaacgcgtac
     3061 gttcaccaat tcttgtgggt aaagcgtcac ttcatcagct aattcagaaa gttttttacc
     3121 ggtctgtttc atgacgttca acaattgaat acccgtcaaa agaccatccc cagtcgtgtt
     3181 catatccagg aagacaacgt gtccggattg ctcgccgccc aaattgtaac catttttgcg
     3241 catctcttcc acaacataac ggtcgccgac ttgcgtcttc aaggccgtca tgccttcagc
     3301 ttccacagcc aaatggaaac ccaggttgct catgactgtc gagacgattg tgttttgatt
     3361 caatttgttg cgctctttca aatatttacc gcagatgaac atgatgcggt cgccgtcgat
     3421 aatgcgtcca ttctcatcta cagcgataca gcgatcggcg tcgccatcaa atgccaagcc
     3481 gacatccgct tttttctcaa cgacaaattt acctaggaca tccggatgag tcgaaccgac
     3541 gcctttgttg atgttgatgc cgtccggatt tgcacccatt gtataaaagt ctgtttccaa
     3601 atccgcgaac agacggttga cgacaggcga agctgccccg tttgccgcat ccaagcagac
     3661 tgtgatgccg gaaagatcat ccggaatggt cgtctgcagg tattgtgcgt atttcaggac
     3721 gccttccgga tattcatcca ccgtgcccaa tccgtctgcg gaaggtctcg gcaaatcatc
     3781 tccttcttgg tccaaatagg cttcgatctc gtattcctgt tcgtccgaca gtttgaagcc
     3841 atccgatccg aagaatttaa taccgttatc gcctgcagga ttatgcgaag cagaaatcat
     3901 gacccctgca acggcacctt gaatgcgtgt caggtaagcc acgcccggtg tcgggatgac
     3961 tcccaattgc aatacttcga taccgactga caacaagccg gaaaccaatg catattccaa
     4021 caattgaccg gaaattctcg tatcacgcgc aaccaacaca agcggatgtg cgatatcttt
     4081 agcgtgctgt cttagaacat atcctccaaa acggcctaat ttgaaagcta attccggcgt
     4141 caattcgctg ttcgcaacgc ctctgacacc gtctgttcca aaatatttac ccattactta
     4201 taatctcctt tagatttttc atttattttc tttgcatgga ttcgtttgat ttttaattta
     4261 tgaactacta tccgactgat cctcttcgct tttcggatta tctgaagccg aagatgcgac
     4321 agcactgctt tcgtcggcgg aagaggagga ctctccttca tcagcggtcg tctccgaatc
     4381 agctggggtc tcggcagcat tcgacacacg gatgtttacc ttgattgttg tcggtgtggc
     4441 cgtcaaagtg tcattcacta ccggaatcgt gagtgtttgg gtggtgttgc tctctacgcc
     4501 tgtgacactg accggcagaa atacctcatc aatatcagca agaatagact tatcgcccgt
     4561 caaggtcaca ctggcttctc catctacgtc aatcgtatag actttacctt caagcggaac
     4621 cccgatctgt tggatgacca acggaacttt tttcccgtac agtgaaatcg gaatgcggac
     4681 ggaaatttcc tgcggatcga cggaaacatt caacttattc ccacttgcat ccactacttg
     4741 tactacagta ttcatattca cggtgtttgt caagccattt tcagctgcga ctgtcacata
     4801 aacactatcc acacggctga tggtcgaagc cggaccagtc aacgttacag tatccacact
     4861 cagttccgga acgcctgcca aatatttagg atctaccaaa gattcatcga agcggacttc
     4921 caccgggctc tcgatggcaa cccgctcttc aatgacgaca ttcactcgtg atggcgtaat
     4981 cgcataatcg atttcattcg atagattctc ggccgtcaat tggatcgtat gcgtccctgg
     5041 ccccaattca tccagattct ccgtcacgat gcggtaatcc gcggccgaaa tcgtctgcac
     5101 gaggacgctc tccggcccat tcaatgtcaa aaccaccgtt tccggcaatc ccgaaataaa
     5161 atattcgtct gtatcgatat tcacctggat cgggacattc gaaatggttt cccgcttgct
     5221 ggtgctgatg gaactattgc tggtgctcgt ggaccagccg tagttttctg agtagacata
     5281 ggtgaacaac aataaggaga tcaagaaaga caggcctctg acgacccact tgttttccag
     5341 aaacttatca tcctgctgtt ttctcattca gaaccacccc atttcgtatt gtcaaagaaa
     5401 tcttgtatga aattggtagt tttttcttcc tcttcctcca caacgaggtg gtctgtcaga
     5461 taggcgacaa aatcatctcg gctcatttcg cgcagcaggc ttgtcttatg ggctatactg
     5521 acaccacctg tttcttccga aacgatgatg gtgatggcat cactgacttc accaagtccg
     5581 attgcggcac gatgccttgt tcccaactct ttcgggatga aagaactctc cgacaagggc
     5641 agataactgg ctgcggttgc gattttgtaa tcctgaataa tgactgctcc gtcatgaaga
     5701 ggcgtattgg gaataaaaat attgatgagc agctgattag tcacttcccc accgattctt
     5761 atccccgtgg cagcgtaccc gtccaacagg tcctgctgct cgatggagat caaagctcca
     5821 atttttcgct ttgccatgta ttggaccgct ttatccagtt ccatcaccaa acgttcactc
     5881 gtattgattg aacgtttggc tttctttctg aatccagtct ttcccaaatg ctccaatcct
     5941 tttcggattt ccggctgaaa aacgacaatg accgccacga caccccactg gatgacgcga
     6001 ttcatgatcc aatctatagt ctgcaactga agcgtgaccg cagccagttt gatcagcaca
     6061 ataatcccga ttcctttcag caattgaatg gaacgtgtac cttttaaaat catgatcaac
     6121 ttgtagacca gataccatac caacaggata tccactatat caaaggcaat gctccaagtg
     6181 atcacttctc cccaatcgat tgacacatgc gcacctccat gtttaaagtt atatcatcaa
     6241 ttatatcaca aataaactta cccaatggca tcgtttcctt gagaaagcgc tttataagcg
     6301 ttacaataga aacagatggg cactcagtca cagctaaagc atatcgtttg caaatccgac
     6361 tcactttgtt atacttagtt tatagttatt acaatatgat aattaattta aattaggagg
     6421 agttcaaatg agtatcgatt ttaaagatca tggcaagaaa ccttacgtgg tgaacatcga
     6481 ggacgcaacc ctgcagaacg agaattaccg gacaacgatc tggaccggcg aaaaattaca
     6541 agtgacggta atgacgatcc aaccgaacga tgacatcggg ctggaggtac accatggcat
     6601 cgaccagttc atccgcatcg aagagggcga cggcgtgtgc gtgatgggcg actcagagga
     6661 cgatctgtac ttcaaacaga aagtttccga cgatgacgct gtctttgtcc ctgccgacaa
     6721 atggcacaac atcaccaaca caggcgacaa accactcaaa ctgtatacga tttatgcagg
     6781 ccctgaccat gtgcccggaa ccgtccaccc gacccatgaa gatgcaaaaa acgacccgaa
     6841 cgaacactaa tgaggaaaag cggaacggga ctgtctcaaa aattgaggca gtcccgttct
     6901 ttttggtttg atggcatgat tgtatagttt ggggattgcg ttcatagcgt acttccccgt
     6961 caaaatggtt ttgacgggga atctcgtgtt gtccgcgaac gtttttcccc gccaaggagg
     7021 ctttggcggg ttttccgact gtgatttgaa ttgattttcc ccgccaacaa gggtttgacg
     7081 ggttatttct gagcgtgaat tcaattccca aatgaaataa aaggggccta tcaaaccaga
     7141 acgtactttt ctggtttgat acagcccctt tattattcac aagaatcaat caatcaattt
     7201 cgctaattgc tcttcaggca agtagaacga tactttgccg cattttggac aaactgccgc
     7261 ttccaaagcc gcttttgtgt gtccgttcaa tgttttttcc ttcgccaatt caacaagata
     7321 aaccccttgc gaatacaaac ctagcccttc aatcaaatcg acgttgcaat ctgtgcattt
     7381 agccatgtat ttcccctgct ttctcttcga taattgtcta tttttttgaa acatacgcct
     7441 catccatcat atcatgtttt tctgatgaaa catttttcca aattatgaac ttttatctca
     7501 agagcgggaa agccgatcag atcgccctag gaggtttgct ggagtggatt attccgttac
     7561 ttctgtcaca tcgacgccat agtatttttc agacaaggct ttgattgtac cgtcagcaag
     7621 caggttctgt aattcttcat ca
//
LOCUS       DAOMUJ010000027         7516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2571_length_7516_cov_2.788902, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000027 DAOMUJ010000000
VERSION     DAOMUJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2571_length_7516_cov_2.788902"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..386)
                     /locus_tag="PLT73_01385"
     CDS             complement(84..386)
                     /locus_tag="PLT73_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19067.1"
                     /translation="MQKGIWRKKLDVADLLERKGCYEFTLLEDKLSVREELFEIWWYA
                     YGGNNHILAKSKRYPTRLYFILLEPGDLFAVDDFRIYLETGELKHPVATYYKRNGR"
     gene            complement(420..662)
                     /locus_tag="PLT73_01390"
     CDS             complement(420..662)
                     /locus_tag="PLT73_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19068.1"
                     /translation="MENGICLKGSVEKIKILKLEETPLIRFTLTVDKEEKQNCLIRAH
                     SLDFLYQVTEGNAIVIYGRKNKRNQVVVQKYHVNQK"
     gene            817..1668
                     /locus_tag="PLT73_01395"
     CDS             817..1668
                     /locus_tag="PLT73_01395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM19069.1"
                     /translation="MAPTGKRSHPTKGRFAMPIKLIAVDMDGTFLDSRKKFNKERFDA
                     LYKRMLEKDIRFVVASGNQYYQLRSFFPEIQDNIAFVAENGAFVVDCNKELAIGEMTQ
                     ETIDKVLRTFKELDACSRLVVCGKNSAYVDSSVSDYFFQNTQRFYHRLKRVDSFSEID
                     DTIFKFASGFAEDEVSALLEHFTREIGDVVSPVSTGHGSIDLIIPGMHKASGIQLLQK
                     LWGISDEETAAFGDSGNDIEMLEHVAYSFAMANAADDVKKAAKFCTLSNNEEGVLAAI
                     EQLLEME"
     gene            complement(1732..2025)
                     /locus_tag="PLT73_01400"
     CDS             complement(1732..2025)
                     /locus_tag="PLT73_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM19070.1"
                     /translation="MEVGSRIQELRKLNKITAKELAEQIDVSPSFISAIEHNSTKLSL
                     KTLNHICEVLGVTLAEFFNTEMSPVEKKLTAQIKKMPEEKKYELLAFLTGLIS"
     gene            2259..3698
                     /locus_tag="PLT73_01405"
     CDS             2259..3698
                     /locus_tag="PLT73_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRM19071.1"
                     /translation="MQQYKQEWFGNIRGDILAGIVVCLALFPEVIGFMIVAGVPPIVG
                     VYATFFITAIAAFFGSSKGMISAAAGSVALVLAHLVAEYGIQYLFAATILAGILQVAL
                     GLLKVGTLMRYIPKPVMTGFVNGLGIMMFTSQLDHFQGSLLLPLLGLLGIAIIFLFPR
                     ITKKIPAPIVAILVITGIVLGFGLDVKTLGDMGSISTDLPRFGIPAVPMTVETLRIIF
                     PTALSVAIVGLVESLLTAQLVDEITEEKSDKNQETLSQGLGNVVSGFFGGIAGCGMIG
                     QTIINLNYGGLGRLATFLSGFFMLLSVVLLNGSVVQIPVVALAAVMVVVSIETINWDS
                     IKRLRTNPKNESFAMILTVAIVIGTHNLAYGVVAGTLVSAVFAAFKMSHLHVATGTAD
                     DDHHYKVDGHLYFASAEKFLDFFAEEIEQEEEIVIDISAMTLWDSTAVEAIDKLITRN
                     NKRGVKTTLTGANTQSSALFKRISIHTEK"
     gene            complement(3971..4657)
                     /locus_tag="PLT73_01410"
     CDS             complement(3971..4657)
                     /locus_tag="PLT73_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM19072.1"
                     /translation="MNSNNEAFMGYFNDIESFFNKTYKPNRGKSYYSFYELVENAARY
                     DSFVKRKRDDFQILGDLRNFLSHGNQSDLVLVNEESLTMIKAIHEQLLKPKTAFDIKS
                     DDVKFFKETTPLSAVLETIRNTDLTQFPIKDGKGKVMGLLTENGITHWLSQHAHEATV
                     SIGGTLARDILVLDDNKDNFAFVKKDASIYEAEALFDNQKIDALLVTHSGKNTENLLG
                     IITRFDLVEV"
     gene            4838..5206
                     /locus_tag="PLT73_01415"
     CDS             4838..5206
                     /locus_tag="PLT73_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19073.1"
                     /translation="MTTANIMLYVEDVEANADFWKHYLNFKEVERYDFGDSISIVLAV
                     SSTCNLQLFNIDFIRKNSPEVASNKPSLLFDISDFDELYARFKENNETVGEIIEMGGA
                     RTFNFPDRDGNYFAVREAAE"
     gene            complement(5308..6030)
                     /locus_tag="PLT73_01420"
     CDS             complement(5308..6030)
                     /locus_tag="PLT73_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRM19074.1"
                     /translation="MNRPYRVICHMAMSIDGRAAGHYLSAVEFGPYGKAYEDTLQAYG
                     SDNWICGRITFEQHITLGNKVDLSIYSDEAIPREDYIHPAAFSTYAIAVDPEGKLGWQ
                     TNVIGDEYPERRGDHIVTILSEAVSDRYLAHLRKVGVSYIFTGKSKPLSMTVALDKLH
                     SHFGIETFMLVGGGFVNGSFASEGLVDEISLIIAPLIEGVSDSVSLFEFGNSVDQRIR
                     KFRLDKFERIGPDGIRLNYLSE"
     gene            complement(6142..6351)
                     /locus_tag="PLT73_01425"
     CDS             complement(6142..6351)
                     /locus_tag="PLT73_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19075.1"
                     /translation="MSINAKQIIEYNESIIEDNIFFGLKNDYTETEINQYVAEEKEEL
                     RRLKKHLKDNGLVEYDNVTQMYVRK"
     gene            complement(6936..>7516)
                     /locus_tag="PLT73_01430"
     CDS             complement(6936..>7516)
                     /locus_tag="PLT73_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM19076.1"
                     /translation="TNVSLNGLPRNHFPNKRALLDLGCYADAFMEKAWRVTKEFNEQR
                     SEYQLMGKGLILEMLGLLLRSLEDGSDNTLPPALSQTAVRQQHLVNHAVYYLENHHSE
                     EITLEQLAQQLFVSPAHLSKIFKEATGLSPIHYLIHVRLKHAKELLKQEEWTIKEVAQ
                     AVGYQDAYHFSKLFKKYYGTAPSQVAKEERTV"
BASE COUNT         1855 a         1881 c         1657 g         2123 t
ORIGIN      
        1 acggctactt tttggctatc ggtctgatgt ttggaatcgg tttggcgatg tatcgttggt
       61 tcaagaaaaa cggctggttc tgattaccgg ccgtttctct tgtaataggt ggcgacagga
      121 tgcttcaatt cccctgtttc cagataaatg cggaaatcat ccaccgcgaa gagatcgcct
      181 ggctccagca aaatgaaata gagccgggtt ggataacgtt tgctttttgc caaaatatga
      241 ttgtttccac cataggcata ccaccaaatt tcaaacagct cctccctgac ggaaagctta
      301 tcctccaaca gtgtgaactc atagcagcct tttcgttcca acagatcagc gacatccaac
      361 ttttttctcc agattccttt ttgcatcaac ctcattttcc tcaccctatc catcagccat
      421 catttctgat ttacatgata tttctgcacc acgacttgat tgcgcttgtt cttcctacca
      481 taaattacga ttgcgttccc ttcggtgacc tgatacaaaa aatccagcga gtgcgcgcga
      541 atcaggcaat tctgtttctc ttccttgtcc accgtcagcg tgaagcggat cagtggcgtc
      601 tcttccagtt tgagaatttt gatcttttcc actgagcctt tcaggcaaat cccattttcc
      661 atatcagtca catcctttaa cacttataat acgaacatgc gttctttttg tcaactgttt
      721 ccttatcgga aaaacggctc aaagcattct tttccttttg ggaataataa gggaacatgt
      781 taagatgttt taaacataag cggatgttaa aagctgatgg ctccaaccgg aaaacgctcg
      841 catccaacga aaggaagatt cgccatgccc atcaaactga tcgccgtcga tatggacgga
      901 acattcctgg acagcaggaa aaagttcaac aaggaacgct ttgatgccct ctataaacgc
      961 atgctcgaaa aggatatccg ttttgtcgtg gccagcggga accaatacta ccaactgcgt
     1021 tcttttttcc ctgaaataca ggataatatt gcttttgtag cggaaaatgg cgctttcgtc
     1081 gtggattgca acaaagaact ggccatcggt gagatgacgc aagagaccat cgataaagtg
     1141 ctccggacct tcaaggaact ggacgcctgt tcgcgattgg tcgtttgcgg caaaaatagt
     1201 gcctatgtcg acagttccgt ttccgattac tttttccaaa acacgcaacg cttctatcat
     1261 cgcctgaagc gggtggattc tttttccgaa atagatgaca ccatcttcaa gtttgcatca
     1321 ggcttcgctg aagatgaagt ctctgccctc ttggagcact tcacacgcga aataggcgat
     1381 gtcgtctccc cggtatcgac cggccatgga tccatcgacc tcatcattcc gggcatgcat
     1441 aaagcttccg gcatccaact gctgcagaag ctatggggca tctccgatga ggagaccgcc
     1501 gcattcggag acagcgggaa tgacatcgag atgttggagc atgtggccta cagtttcgcg
     1561 atggcgaacg cggccgatga cgtcaaaaag gccgccaaat tctgtaccct ttcgaacaac
     1621 gaagaaggcg tgttggctgc aatcgaacaa ctgctcgaaa tggagtaaac gaaaaaggaa
     1681 gatgtccccg cctcatattg aagcgggatg catcttccct ttctattctt atcaggaaat
     1741 caagcctgtc aaaaaggcca ataattcata ctttttctct tccggcattt ttttgatttg
     1801 agcagtcagt tttttttcca cagggctcat ttccgtattg aaaaattccg ccaaggtgac
     1861 cccaagtacc tcacagatat ggttgagcgt cttcaaggac aatttagtcg aattgtgttc
     1921 gatggctgaa atgaaggaag gcgaaacgtc gatctgctca gccaattctt tcgccgttat
     1981 tttgttcagt ttgcgtaatt cctgaatacg actgcctact tccatgcgta tctgatcctc
     2041 tttcctttga ttcaacattt ctttttacat actagcacac cccagcttaa aatttaaagc
     2101 tttttaactc ttttttaaac gtatcattca aaatatacac ctatgaataa atttttatcc
     2161 ccctttattc tgttatgtgt taacatatat ttttgtgagc acgttcaata attttttaaa
     2221 tcaaaataga cactattttt tcaagaagga aggtatttat gcaacaatac aagcaagaat
     2281 ggttcggcaa tatcagaggg gatatcctag ccggtatcgt cgtctgtctg gcgcttttcc
     2341 cagaagtcat cggcttcatg atcgtcgcag gggtgccacc gattgtcgga gtatacgcca
     2401 cttttttcat cacagccatc gcagcctttt tcggctccag caaaggcatg atttcagccg
     2461 ctgctgggtc agttgccttg gtcctggccc atctggtcgc tgaatacggc atccagtacc
     2521 tttttgctgc tacgattttg gcaggtatcc tgcaggtcgc gcttggctta ttaaaggtag
     2581 gcaccttgat gcgctacatt ccgaagcctg tcatgaccgg tttcgtaaac ggcttgggga
     2641 tcatgatgtt cacttcgcaa ttggatcatt tccaaggcag cctgcttttg ccgctcctgg
     2701 gtctgttggg aatcgcgatc attttcctgt ttcctagaat tacgaagaaa atccctgcgc
     2761 ctatcgttgc gattctcgtc atcaccggga ttgtgctggg ctttggcttg gatgtgaaaa
     2821 cgttgggaga tatgggttcc atctctacgg atttgccgcg cttcggcatc ccggctgttc
     2881 cgatgacagt ggaaactttg cggatcattt tcccaactgc actttcggtt gctatcgtcg
     2941 gattggttga gtccttgttg actgcacaat tggtcgatga aatcactgaa gagaagagcg
     3001 acaaaaatca ggaaacgttg agccaaggtc tcgggaatgt cgtttccgga ttcttcggcg
     3061 gtatcgccgg ttgtggcatg atcggtcaaa caatcatcaa cctgaattac ggcggccttg
     3121 gccgtcttgc gactttcctg tccggattct tcatgttgtt gtccgttgtt cttttaaacg
     3181 gttctgtcgt gcagattccg gttgtcgctt tggcggcggt catggtcgtt gtctctattg
     3241 aaaccatcaa ttgggattcg atcaaacgtt tgcgcacgaa ccctaaaaac gaaagcttcg
     3301 caatgatcct gaccgtcgcc atcgttatcg gaacgcacaa cttggcttac ggtgtcgtag
     3361 ccggtacttt ggtcagcgcc gtcttcgcag ctttcaaaat gtcccatctc catgttgcaa
     3421 cagggactgc tgatgatgat caccactaca aagtggacgg ccacctttac ttcgcttccg
     3481 ccgaaaaatt cctggacttt ttcgctgaag agatcgaaca ggaggaagaa atcgtcatcg
     3541 acatcagcgc catgactcta tgggattcca ctgctgtcga agcgatcgac aagctcatta
     3601 cccgcaacaa caaacggggt gtaaaaacga cgcttaccgg tgcgaacacg caaagttccg
     3661 ctcttttcaa acggatttcg atccacacgg aaaaataaga aaactcacag tctgcctctc
     3721 gaggcggcct tttatcatct tcttcctccg gcgagcgtga tcttcgccgg aggacggcta
     3781 tcattccgag ttgcgttctc cgatgaacca agcttaatcg gaggtgaggc tgattgttac
     3841 aggttgcctc cggcgaaggg gtcactcaca ggagctgcgt atacatttcg gcgctctgtt
     3901 ccaaaaaaga caggcattcc cacaaaaatg ggtagcctgt ctttgtcgta ttttctgaac
     3961 taaaatctat ctacacttcc accaggtcaa atcgtgtaat gatgccaagc agattttccg
     4021 tatttttgcc gctgtgcgtc accagcaatg catcgatttt ttggttgtcg aatagcgctt
     4081 ccgcctcata aatgctcgca tcttttttga cgaaggcaaa attatccttg ttatcgtcca
     4141 atacaaggat atcgcgtgcc agcgttccgc cgatcgacac ggttgcctca tgcgcatgtt
     4201 gggacagcca gtgcgtgatg ccattttctg tcaacaagcc catcactttt ccttttccgt
     4261 ctttaatcgg gaattgcgtc aaatcagtgt tcctgatcgt ttcgaggaca gcggatagcg
     4321 gggtagtttc cttgaagaat ttcacatcat ccgatttgat gtcgaaagcc gtctttggtt
     4381 tcaacagttg ttcatggatt gctttgatca tagtcagtga ctcctcattg acgagcacca
     4441 gatcgctttg gtttccgtgg ctcaagaaat tgcgcagatc ccctagtatc tggaagtcat
     4501 cccgcttcct tttcacaaaa ctgtcgtagc gcgcggcgtt ctccaccaac tcataaaagc
     4561 tatagtagga tttcccgcga ttgggcttgt acgtcttgtt gaagaaactc tcgatatcat
     4621 tgaaataacc cataaatgct tcgttattgc tattcaatcg ccttcaccct ctctcaaatt
     4681 gatctcgtta tgcttctatt ctaacaagtc aaagcgggaa atacgaatcc aaagcctttc
     4741 atccaaagac gcttgctgca accgttcttc acattggcct ccgaattact ccgagcgtat
     4801 aatgatatta tgatcgataa gaaacggagg aaatgatatg accacagcga atatcatgct
     4861 ttacgtagag gatgtagaag cgaatgccga tttttggaaa cactatctca atttcaagga
     4921 ggttgagcgt tatgactttg gggattccat cagcatcgtg ctggcggtga gctctacgtg
     4981 caatctgcag cttttcaata tcgatttcat cagaaagaat tcaccggaag tggcctccaa
     5041 caaaccttcc ttattgttcg acatctccga tttcgatgag ctttatgcgc gcttcaagga
     5101 gaacaacgag acagtcggtg agatcatcga aatgggcggc gccaggactt tcaacttccc
     5161 tgacagggac gggaactact tcgccgtccg ggaggcggct gaataagcag accacgcgct
     5221 ccgaacaaag tttagtcaaa gaaccaccga atccaaaggc aaaacgcctt ggatccggtg
     5281 gttctttcgt tctgcatgac cctcgtttca ctccgaaaga taattcagcc ggatgccatc
     5341 cggaccgatc cgctcgaatt tatccagcct gaacttccgg atacgctgat caacactgtt
     5401 cccgaactcg aataaagaaa cggaatcgga aacgccttcg ataagtggag cgatgatgag
     5461 gctgatttca tcgaccaagc cttcgctcgc gaacgatccg ttcacaaatc ctccgccgac
     5521 cagcatgaaa gtttcgatgc caaaatggct gtgcagtttg tccaaggcga ctgtcatcga
     5581 caatggtttg ctcttccctg taaagatgta ggaaacgccc actttcctga gatgggccag
     5641 ataccgatca gagaccgcct cgctcaaaat cgtaacgata tggtcgcctc ttctttccgg
     5701 atactcgtca ccgatcacat tcgtttgcca gccgagtttc ccttccggat cgaccgcaat
     5761 cgcgtaagtg ctgaaggcag ccggatgaat gtaatcttcc cttgggatgg cttcatccga
     5821 ataaatggac aggtccactt tgttcccgag ggtgatgtgc tgctcgaacg tgatccgccc
     5881 gcagatccaa ttgtccgaac cgtaagcttg tagcgtatcc tcataggcct ttccgtacgg
     5941 cccgaattcg accgcactta aatagtgccc cgccgcgcgc ccgtctatcg acatcgccat
     6001 gtggcaaatc actctgtaag gtctgttcat ttctttttcc tccattctga atcttagttg
     6061 atgattccat cataaaccct tgaggttagt ttaagtcaag aagaaagcgc aaatttggcc
     6121 cacatctatc aaatcacgtt tctactttcg tacatacatc tgtgttacat tgtcatattc
     6181 gaccaaacca ttatctttca ggtgtttttt taggcgtcgc aattcttcct tttcctctgc
     6241 cacatattga tttatctcgg tttccgtgta atcattcttc aatccaaaaa aaatattatc
     6301 ttcaatgatc gactcattat actcaatgat ttgttttgca tttattgaca caaaaatcac
     6361 ctctttcttt aaggctaaac atttctcttg atgcgtttcc agcaattcag cacaacggtt
     6421 tactgatttc ttgaaaaact ttattaaaac gtttatttaa agcgttgttc aagagtatca
     6481 tccgcagata tcaaggaaaa gatacaaaat aacagttggc agggttacgg ttgcttcgat
     6541 tccgcaaaga agaactccca tctaacgaac taaaccatga gctttcttac ttgaagagtg
     6601 atatcaggca cggtcaagtc aagaagggcc tgtcagtaat gattattctc gaatccgatc
     6661 cgatgctcct ctggccggag ggatcggaat ctgattatag ctccggtgag gctcccgctc
     6721 gcaggaggac aacgctaact atcggcacct tctccggcga agtcatcctc atcggagaac
     6781 aactgaaata aaatcctaaa ctccgaccaa gcctcggttc aacggaactg agggccggat
     6841 cttgcgttca ccgttatgcc atcaggtatc cggaagtttc caacaaaaag agccgcctca
     6901 acttgagtcg gctccttttc ttgttattga tactcctata ccgttcgctc ttccttagcg
     6961 acttgcgaag gcgcggttcc gtagtatttt ttgaacaatt tgctgaagtg gtacgcgtcc
     7021 tgatagccga cagcctgggc gacttctttg atggtccatt cttcctgctt cagcagttcc
     7081 ttcgcatgct tcaggcgcac gtggatcagg taatggatag ggctcaatcc ggtcgcttcc
     7141 ttgaagattt ttgaaagatg ggccgggctg acgaagagtt gttgcgccaa ttgttccaac
     7201 gtgatttctt cggaatggtg gttttccaga taatagacgg catggttcac cagatgctgt
     7261 tgacgcacgg cagtttggga aagcgccggc ggaagcgtat tatcggatcc gtcctccaga
     7321 ctgcgcagca acaaccccag catctccaat atcagccctt tgcccatcag ctgatattcg
     7381 ctccgctgct cgttgaactc cttcgtcacc cgccaggcct tctccatgaa ggcatccgca
     7441 taacagccaa gatccagcaa tgcgcgtttg ttcggaaaat gattacgggg aagtccgttc
     7501 agtgagacgt ttgtca
//
LOCUS       DAOMUJ010000028         7497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2584_length_7497_cov_2.837678, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000028 DAOMUJ010000000
VERSION     DAOMUJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2584_length_7497_cov_2.837678"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /gene="ribA"
                     /locus_tag="PLT73_01435"
     CDS             <1..977
                     /gene="ribA"
                     /locus_tag="PLT73_01435"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRM19077.1"
                     /translation="RNTDNHGTAFTVAIDHVETTTGISAFERSLTVMKAVEDDAQPED
                     FRRPGHIFPLKAVDNGVLARNGHTEATVDLARLAGLKPAGLCCEIMADDGTMMRTAEL
                     ITFAKEHDLKIGTIADLIAYRKENEQLYKREGQAKLPTKYGEFDIYTYVSKIDADEHH
                     VALVMGDVTTEEPVLVRVHSECLTGDVFGSKRCDCGQQLDAAMKQIAEAGRGVLVYLR
                     QEGRGIGLVNKIHAYALQDQGYDTVDANLMLGFPSDLREYYMADEMLQDLGVKEIRLL
                     TNNPLKIEGLKKHGLKVIERIPIQLEIFGETQRYMETKKQKMGHYLDL"
     gene            1006..1467
                     /gene="ribE"
                     /locus_tag="PLT73_01440"
     CDS             1006..1467
                     /gene="ribE"
                     /locus_tag="PLT73_01440"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRM19078.1"
                     /translation="MNIIEGQLIAQDLKIAIVVARFNEFIGSKLVGGALDGLKRHGVS
                     EDAIDLAWVPGAYEIPLVAQKMAASGKYDAVITLGAVIKGATAHFDYVCSEVSKGVAT
                     ASMNTGVPVIFGVLTTDTIEQAIERAGTKAGNKGYDCAVSAIEMANLLKNL"
     gene            1486..2139
                     /locus_tag="PLT73_01445"
     CDS             1486..2139
                     /locus_tag="PLT73_01445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM19079.1"
                     /translation="MFKGVHTIILDYDGTIHDSTRNYIYAFKQAYAFLVEAGHAAPRD
                     WADAEITKWLGYSSKAMWENFMPDLDEDIRSKASKMIGQILLEKAQNGQSILYEGALE
                     TLQALKEKGYRLIFLSNCSRAYMEAHRESFGLDRYFDGMYCTEDFSFIPKYEIFESIE
                     SVFPGGYLIVGDRFQDIEVAEHHPVRTVGCRYGFGFPGELESADVLIDDIRELNDLL"
     gene            complement(2440..3120)
                     /locus_tag="PLT73_01450"
     CDS             complement(2440..3120)
                     /locus_tag="PLT73_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRM19080.1"
                     /translation="MEIAILQHVDFEGPAEIENWCRQTGNEYKVYRLFLGEALPKAER
                     TDFLVILGGPMSVLDELPWLEEERQLIRVLISQGKPVFGVCLGGQQIAKTLGADIFQG
                     EYREAGWLPIRTMKSETLGELPEELVVFHWHGEQFGLPEGAERLFTSEVCPNQGFIYK
                     ENVIGLQFHFESTKESIESILQNDAAFLDGTAYTQTSAEIRNYPIPASNRKLLYRLLD
                     RLKATVPD"
     gene            3278..3586
                     /locus_tag="PLT73_01455"
     CDS             3278..3586
                     /locus_tag="PLT73_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19081.1"
                     /translation="MRNLDKEIAIIQKFKAEHPDKAYKLYIETSDDFDFHDETESEDY
                     IAVLIAELETETPVENPFLEGTSAYTWDAEYVIYIDKPTTADRELITKLYYDELLQYV
                     "
     gene            complement(3593..3772)
                     /locus_tag="PLT73_01460"
     CDS             complement(3593..3772)
                     /locus_tag="PLT73_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19082.1"
                     /translation="MAELDRLVKLLVEKLLRSSKKPEPSGSGTDEGKELGHKPLERVS
                     EGTAKDETDEKMPEG"
     gene            3949..4350
                     /locus_tag="PLT73_01465"
     CDS             3949..4350
                     /locus_tag="PLT73_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM19083.1"
                     /translation="MSQNFENAIRERFEQGFKNWNNGYDAWLDWCETLYEPDAHYNVY
                     SDRLTLQQYKDMMGQLFQAYDIELGAFHNMIVEGEWGAIRYNVYIIDKRTGERIEQKT
                     MEFGKFKENPEPIGVRVIEGWALSDKPLSAH"
     gene            complement(4483..4674)
                     /locus_tag="PLT73_01470"
                     /pseudo
     CDS             complement(4483..4674)
                     /locus_tag="PLT73_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011677428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(4668..6479)
                     /locus_tag="PLT73_01475"
     CDS             complement(4668..6479)
                     /locus_tag="PLT73_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM19084.1"
                     /translation="MDKFLKRNPSLFHQIEQGKAAILYPGDGMHMLLLGETGVGKSLF
                     ADLLHKYGIQSGKLAEASPFIQFNCADYTHNPQLLMSQLFGHVKGAYTGAETNKTGLL
                     EKANGGILFLDEIHRLPIEGQEMLFTYIDRGVFRRLGESEKDHHANVLIIGATTEKKD
                     ALLKTFIRRLPIIIDLPSLRERSIEERHQLFDTFVQQEVDQLGVPMYFSKNALKAFLH
                     YKCELNIGQFKADIRIAFAKSYARFLAVKEDYLRVSSKDLPDYIWAGLLSNAAHRQMW
                     SKYEVTNRQAIQYLPSTATVTEVSVPSESTIYREMNTRYRELEQNKASAEEIQKAMEH
                     DLKTYFKLTGEKEQLFCNKENLIALVPDNIIKELEKILRHVTIKTGKVVSDKVFQMMA
                     IHIYNLVKRSQQDHVLKNDMHEAIIDKYPEMYGVAKECMTILEADLGVVIPEEEASYF
                     IMFLMHDEMDAIERLAKVQVIVIAHGSGIASALCDTANQLLNHEGAIGINARLDEEPQ
                     QVFERLVRYIKNHQITDDILLLVDMGSLTTFAGELEKLFPLKAKSIPLVSTMHVLEAS
                     RKAMIGCGLREVYVETLKVNDYMAIYQDRKEVEQLQI"
     gene            6955..>7497
                     /locus_tag="PLT73_01480"
     CDS             6955..>7497
                     /locus_tag="PLT73_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM19085.1"
                     /translation="MISILLVRKIAQLFLIMILGYLLVKLRILKTEESIVLSKLSLYL
                     VMPAVILSAFQVDFKPEIQAGLMLAFVAAVAIHILLLIIGHISGSLFHFEEIDIASII
                     YSNAGNLIIPIVTAVLGTEWVIYSTAFLSVQLIFLWTHCKLMFSKEKKPHFRKIILNV
                     NMIAIFIGVLSMLSGSRLPAI"
BASE COUNT         1905 a         1957 c         1723 g         1912 t
ORIGIN      
        1 accgcaacac cgataatcat gggacagcct tcaccgtcgc catcgaccat gtcgaaacga
       61 caaccggcat ctcggccttc gaacgctctt tgaccgtcat gaaggcagtc gaggatgacg
      121 cacagcccga ggatttccgc agaccagggc acatcttccc gctgaaagca gtggacaacg
      181 gtgtactcgc gcgcaacggc cacaccgaag ccaccgtcga cttggcgcgc ttggccgggc
      241 tgaagccggc cggtctctgc tgcgaaatca tggccgatga cggcacaatg atgcgcacag
      301 ccgaactgat cactttcgcc aaagagcatg acctgaagat cggcacgatc gctgacctga
      361 tcgcttatcg caaagagaac gagcagctct acaaacgtga aggccaggcc aagctgccca
      421 caaaatacgg tgagttcgac atctacactt acgtgagcaa aatcgacgcg gatgagcatc
      481 acgttgcctt ggtcatgggc gatgtcacga cggaagaacc cgttctggtc cgcgtccatt
      541 ccgaatgctt gaccggcgac gtcttcggat cgaaacgctg cgactgtggc cagcaattgg
      601 acgcggccat gaagcagatc gccgaagcag gtcgcggagt tttggtctat ttgcgccagg
      661 aaggtcgcgg catcggcctc gtcaataaaa tccatgctta tgcgctgcag gaccaaggtt
      721 acgacacggt tgacgccaat ctgatgctcg gattccccag tgatctgcgc gaatactaca
      781 tggctgatga gatgcttcag gatttgggcg tcaaagagat ccggcttttg acgaataacc
      841 cgcttaaaat agaaggatta aaaaagcacg gcctgaaagt catcgaacga atcccgatcc
      901 agctggaaat tttcggcgaa acgcaacgct acatggaaac aaagaagcaa aagatggggc
      961 attatttaga tttataaaaa aacaaccaaa caggaggaaa acaaaatgaa catcatcgaa
     1021 ggacaattaa ttgcacagga tttaaaaatc gctatcgtgg tggcacgttt caacgaattc
     1081 atcggctcaa aattggtggg cggcgctttg gacggcctga aacgccatgg tgtgtctgaa
     1141 gatgccatcg acttggcatg ggtaccaggc gcatacgaaa tcccgttggt agcgcaaaaa
     1201 atggcagctt ccggaaaata tgatgccgtc atcactttgg gcgctgtcat caagggtgca
     1261 acggcacatt tcgattacgt ctgttccgaa gtgtccaaag gcgtagcgac agcttccatg
     1321 aataccggcg ttccggtcat cttcggtgtc ctgacgaccg acacgatcga acaagccatc
     1381 gagcgcgccg gtacgaaagc gggcaataaa ggctatgatt gtgctgtatc tgccatcgaa
     1441 atggccaacc tgctcaaaaa tttataaaaa attatgggaa aacgaatgtt caaaggtgtt
     1501 cacaccatca tccttgacta cgacggcact atccatgaca gtacccggaa ttacatctac
     1561 gctttcaaac aagcctatgc ttttcttgtt gaagccggcc acgctgctcc cagggattgg
     1621 gcggatgcag aaatcacgaa gtggctgggc tacagcagca aagcgatgtg ggaaaatttc
     1681 atgcccgact tggacgaaga catccgatcg aaggcctcaa aaatgatcgg gcagattctg
     1741 ctggaaaaag ctcaaaatgg gcagtctatt ctttacgaag gcgctctgga aaccttgcag
     1801 gcattgaagg aaaaaggcta tcgcctaata tttttgagca actgcagccg ggcctatatg
     1861 gaagcccatc gggaaagctt cggcttggac cgttacttcg acggcatgta ctgcaccgaa
     1921 gacttcagct tcatcccaaa atatgaaatc ttcgaatcga tcgaatctgt tttccctggc
     1981 ggctacctga ttgtgggcga ccgtttccag gacatcgaag tcgccgaaca tcatcccgtc
     2041 cgcacagtgg gctgccgtta cggcttcggt ttccccgggg aactggaatc ggcggatgtg
     2101 ctgatagacg atatccgcga attgaatgac ttgctttaac aatcaaaaga ggctgggatt
     2161 ttgtcccagc ctctttttgt gcttcgcgga tatgaggtgc ctaaacatgg aagagcctac
     2221 ataaacaggc ttcctcggct gtttgtaaaa ctatgattgc atatcagccg acacaaagaa
     2281 gattccttga ctgttcgcaa agtagcgact gcacatcagc cgacacaacg ggactcctcg
     2341 gctattcgca aggaagcgca tgacggacaa ccgacgaagc acacttccac cggaaattcc
     2401 gattccccca tcttatcgat ctcgccggat ttatgctgtt caatccggta ccgtagcttt
     2461 taaccgatcc agcaagcgat aaagcagctt ccggttgctc gcgggtatcg gataattccg
     2521 aatttctgct gacgtctgcg tataggcggt gccgtccaag aaggccgcat cgttctggag
     2581 aatgctctcg atgctctcct ttgtggactc gaaatggaac tgcagaccga tcacattttc
     2641 tttatagata aaaccctgat tcggacagac ttcgcttgtg aacaggcgct ccgctccttc
     2701 cggcaagccg aattgctccc cgtgccaatg gaacacgacc agttcctcag gcagttcgcc
     2761 tagcgtttcc gatttcatcg tccggatcgg cagccaacct gcttcccggt attccccctg
     2821 aaaaatgtca gcccccaaag ttttggcgat ctgctgtccg cccagacaaa ctccgaagac
     2881 cggctttccc tggctgatga gtacacggat aagctgcctt tcctcttcca gccaaggtag
     2941 ttcatccagg acgctcatgg gaccgcccaa tatcacaagg aaatccgttc tctccgcttt
     3001 cggcaaagct tcccccagaa ataggcggta taccttatat tcgtttccgg tctgccggca
     3061 ccaattctcg atctccgccg gcccttcaaa atccacgtgc tgtaaaattg cgatttccat
     3121 gctgatccct ccttaaaatt gattattcca tcataatata tgagcccgca accgtcaccc
     3181 atcgccgggt tattgagtgc agatgcaggt ggagaatgtt atgatagacc tatagaaaac
     3241 acttgatcca aagccatttc cgaagggagc aagcaccatg agaaacctgg acaaagaaat
     3301 tgcgatcatc caaaaattca aagccgagca cccggacaaa gcctacaaac tctacatcga
     3361 gacaagcgat gacttcgact tccatgacga aaccgaaagc gaagactaca tcgccgtcct
     3421 catcgccgaa ttggaaaccg aaacgcccgt agaaaatccg tttttggaag gcacatccgc
     3481 ctacacttgg gatgccgaat acgtcatcta catcgacaaa cccactaccg ctgaccggga
     3541 attgatcacg aagctttatt atgatgaact gctccaatat gtctgaagga aatcatcctt
     3601 caggcatttt ttcgtccgtt tcgtctttag cagttccctc cgaaacacgc tctaggggct
     3661 tatgccctaa ttcttttccc tcatcggtcc cactcccgct tggctccggc tttttggatg
     3721 aacgcagcag cttctccacg agcagtttga ccaagcgatc cagttcagcc atcacaatcc
     3781 ctcccctgct tttttcatta tcatacaata tccgcccgaa tatttcggga tatttttgta
     3841 ctttttggtc cccatctcaa agtttacgtt cagtttatcc cctgttgata tagtcatacc
     3901 aaggtttaca gaacacaaaa agtagaccaa atcgaaggag gaaaaataat gagtcagaat
     3961 ttcgagaatg cgatcagaga gcggtttgag caaggcttta agaattggaa caatggctac
     4021 gatgcttggt tggactggtg cgagactttg tatgaaccgg atgcgcacta taatgtctat
     4081 agtgatcgct tgaccctgca gcaatacaag gacatgatgg ggcaactttt ccaggcctat
     4141 gacatcgaac tgggcgcatt ccataatatg atcgtagaag gtgaatgggg agctatccgt
     4201 tataacgtct atatcatcga caaacgtact ggcgaacgga tcgaacagaa gacgatggag
     4261 ttcggcaaat tcaaagaaaa tcccgaaccg atcggggtac gcgtcattga aggttgggca
     4321 ttgtccgaca aaccgttgtc cgcccactaa taataaaaca tttaccgcct caagataaaa
     4381 caaaagcagc ctccagaaat ccccatcata cggactccgg aggctgttca ttttatctta
     4441 gaatcggcaa actgctcagt tgttcaagga ctacgtgtat attcagtcca gatcagcccc
     4501 gtttgaagcg atgatctttt tgtaccagtg gaacgaatct tttttgctgc gtttcagtct
     4561 gtctattcct tcatcatcct gatcaacata tatgaagccg taaagcttgg acatttcgct
     4621 cgtgctcgtg ctgatcagat cgatgcagcc cgatggcatg taactcatca gatttgcaac
     4681 tgctccactt ccttcctgtc ctgatagatg gccatatagt catttacctt cagtgtttcg
     4741 acgtagactt cacgcagacc gcaaccgatc atcgcttttc tgctggcttc gaggacatgc
     4801 atggtgctca cgagcggaat ggacttagct ttcagaggga acagtttctc caattccccc
     4861 gcaaaagtcg tcaggctgcc catgtccacc agcagcagga tgtcatcggt gatctgatgg
     4921 tttttgatgt aacggaccag cctttcgaac acttgctggg gctcctcatc caaacgcgca
     4981 ttgattccga tggccccttc gtggttcaac agctggttcg ccgtgtcgca aagtgccgag
     5041 gcaatccccg atccgtgcgc aatcacaatg acctgcactt tcgccaatct ttcgatcgca
     5101 tccatctcat cgtgcatcag gaacatgatg aaataactcg cttcctcttc cggaataacc
     5161 acaccaagat ccgcttccag aatagtcatg cactccttcg cgacgccgta catctccgga
     5221 tacttgtcga tgatcgcttc atgcatgtca ttcttcagca catggtcttg ttggcttcgc
     5281 ttcacaaggt tgtagatatg aatagccatc atctggaaaa ccttgtcgct cactacctta
     5341 cccgtcttaa tggtgacatg gcggaggatt ttctccagtt ccttgatgat gttatccggc
     5401 acaagtgcga ttaggttttc tttgttgcaa aaaagttgtt ccttttctcc ggtcaatttg
     5461 aaataagtct tcagatcatg ctccatcgct ttttggatct cttccgcgct ggctttgttc
     5521 tgctccaatt cccgatagcg tgtgttcatt tcccggtaga tggtgctttc actcggaacg
     5581 ctgacttccg taacggtagc ggtggatggc aaatattgga tcgcctgtcg gttcgtaact
     5641 tcgtatttcg accacatctg gcggtgagcc gcattgctga gcagccccgc ccagatataa
     5701 tccggcaaat ctttactgct gactctcaga taatcctctt tgactgccaa aaaccgggca
     5761 tagcttttcg cgaaagcgat ccggatatct gctttgaatt gcccaatatt caattcacac
     5821 ttgtagtgca gaaaagcttt cagcgcattc ttgctgaaat acatcggcac ccccaactga
     5881 tctacttcct gttggacgaa tgtatcgaac aactgatgtc tttcttcgat tgacctttcc
     5941 ctcaaactgg gcagatcgat gatgatcggc agtctgcgga tgaatgtctt caaaagtgcg
     6001 tccttttttt cagtcgtcgc gccgatgatc aggacattgg catgatgatc tttttcggat
     6061 tcgccgagcc gccggaaaac accgcggtcg atataggtga acagcatctc ctgtccttcg
     6121 atcggtaacc ggtggatttc atccagaaac aaaatcccgc cgttcgcctt ctccagaagt
     6181 cccgtcttgt tggtctccgc cccggtgtag gcgcctttga cgtggccaaa cagttggctc
     6241 atcaacagct gcgggttatg ggtataatca gcgcaattga actggatgaa cggcgaggcc
     6301 tccgccagtt ttccggactg gatgccgtat ttgtgcagca aatcggcgaa caaagatttc
     6361 cctacgccag tctcgcccag caacagcata tgcatgccgt cccccggata aaggatggca
     6421 gccttccctt gttcgatctg atggaacagg ctcggattac gcttcaggaa cttgtccatg
     6481 tccaccattt taggcgtttc cttcggtacc ccagtctttg gtaccctgtt gtgggtgcat
     6541 ggaaaaaagc gtaccggtct ggtcttctct ttgtagcagc gctgttcttt gtgcaaccgg
     6601 ttcaggtcgc gcgaaatatt gctgcgatca ctctgcaacc tgtaggcaat ctcttcggcc
     6661 gttatcccct gcttcttctc ttcataaagc atgcccagtg cctcaagcac tttctcgatc
     6721 ctctgcaaaa atcatcactc ctcagaaaag cgtttacatc ttcaacaatc gtaccatatt
     6781 tttatgacag acgaatacaa aaacgttcaa tctgacctat tgttttgctc tgtttgagtg
     6841 ctaaggcgaa aattggcaaa gatgaaagta aaaatggaca tattgcccca atccttttct
     6901 gatcagatac gatataatga actctaaatt cttaactaca aaagaggact gcacatgatt
     6961 agtatcttat tggttcggaa aattgcccaa ctgttcctca tcatgatttt gggttatctg
     7021 ttggtaaaac tgagaattct gaaaacggaa gagagtatcg tcttgtcgaa actatcgttg
     7081 tatctagtga tgcctgccgt gatactcagc gcttttcagg tcgacttcaa gccggaaatc
     7141 caagcaggct tgatgctggc ctttgtggcg gctgttgcca tccacatact cctgctcata
     7201 atcggacaca tctcgggtag tctgttccat tttgaggaaa ttgatattgc gtccatcatc
     7261 tattccaatg caggaaattt gatcattccc attgtaacag ccgttttggg gactgaatgg
     7321 gtcatctatt ccacggcatt tctgtccgtt caattgattt tcctgtggac gcactgcaaa
     7381 ttgatgttct caaaagaaaa gaaacctcat tttcggaaaa tcattctcaa tgtaaacatg
     7441 atcgccattt ttattggcgt gctgtccatg ttgtcgggaa gccgcttgcc ggccatc
//
LOCUS       DAOMUJ010000029         7465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2598_length_7465_cov_2.646694, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000029 DAOMUJ010000000
VERSION     DAOMUJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_7465_cov_2.646694"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLT73_01485"
     CDS             complement(<1..191)
                     /locus_tag="PLT73_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM19086.1"
                     /translation="MLKNWKKVSLATVLLSGLVLGACGNGSDGEAGSGDVTITYSIWD
                     KIQQPGMEAIAEAFEADNPG"
     gene            complement(648..1529)
                     /locus_tag="PLT73_01490"
     CDS             complement(648..1529)
                     /locus_tag="PLT73_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM19087.1"
                     /translation="MGAAATTEIRTEVMEMDEDFFKEEIYYKDLYYTTKGKEQCAPNH
                     SFGPTVREYFLIHIIMKGKGYFEIGNSRFNLKEGQFFIIYPNVMTYYQADEEDPWEYY
                     WIGMKGEHLEALLTAIGFQVSHPVGNVKNFGQVKAMLQEIMAANPFDTLSRLRLQGQL
                     YLFLSMLAHDLDGTEIKQIEKINKGVEYTKRAIEIIKDRYQDSDFLIGDISRELSLND
                     SYLTAVFHKTTNRTPHEYLIDFRIQKSREYLETTDLSIAEIAEKVGYQNPLSFTRIFK
                     SKMQLSPRVYVKKVKTK"
     gene            1716..2480
                     /locus_tag="PLT73_01495"
     CDS             1716..2480
                     /locus_tag="PLT73_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19088.1"
                     /translation="MKKIILSALLLLPLFLLSACTTALPGEEVMQNLVNRSADLDSYH
                     QFVAFRTTTTKEDLNGATTVNEQYTTADATLFPEEKTGYGKRIDKNSDYPATKSEFYV
                     TPEVGYVRTDDADWTAYATPDVPLASLQVYPLETFIELSQIIETYGTLERKGDEYVLH
                     FSGSDDALNKEINYLFQTFPAERTDYDIEIRLDAETFYLSDFSYRSIVTQADQKAVST
                     TELTGEFDLYDQAKKLKSVPVFDSASSVPETSDSSF"
     gene            2634..3005
                     /locus_tag="PLT73_01500"
     CDS             2634..3005
                     /locus_tag="PLT73_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM19089.1"
                     /translation="MIFDKRSPVYEQIIEMHKQKIVSGAFQPGQEIPSRRELATQLKV
                     NPNTVQRAYKEMEGLGLIYTDGNSLSKITESQSKIQTLRQELLDEALQGFIETVRTIG
                     LEDEAVLDLIKKRLKEVHVND"
     gene            2998..3687
                     /locus_tag="PLT73_01505"
     CDS             2998..3687
                     /locus_tag="PLT73_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19090.1"
                     /translation="MIEIKNLEKKYGKRTILEDVSFTIKKNEVTCLTGMNGTGKTTLM
                     NCIMGLVPRNNGSITIDGQDIHKQLYEKISYIPDSLTMPRWMTIAESMKFMSTYYPNW
                     NAAKADELLSFFRLESDLQLKNLSKGNSAKVSFLLGLSLDADYYLMDEPFSGIDVFAR
                     EDILEVFTSKFVTDKGVLIATHHMDEVEMLLDRIVMLRSGRVVKDFYAEDIRQNEGKA
                     IIDVMREVYQP"
     gene            3684..4511
                     /locus_tag="PLT73_01510"
     CDS             3684..4511
                     /locus_tag="PLT73_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19091.1"
                     /translation="MKKFMELWIFEWNRAKRFYGFLLAFVTALQAYAVFHEYHIWKDG
                     YENYRQQNYAALPEQYLQDYGYLTLEDVTNNSFFVFSIMTAIFALLFYAVFIWYQDWS
                     GKNRFSFRLLSLPGNRFAVYAAKFATIWLLITGLQVWQIGLLYLEELVFSGLIPADIY
                     REIPLQMASTSASPLALALPSLFSNYLLFYTIGYTALTLGYTFILMHLSGHWKGVFLA
                     ATPAIVLFAAMLLFLRTGITTRLYAQEKYWMTIAVSLMLLLSGTWANYRLLEKRISV"
     gene            4526..5695
                     /locus_tag="PLT73_01515"
     CDS             4526..5695
                     /locus_tag="PLT73_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19092.1"
                     /translation="MRKQTILLLSLLLLTVLGGFYVQPLLAKEEPLRIGIETVSGDEA
                     VLDGLLYYGTAHSQWETTTLSFDSSGLRHSETQRQDAFFQDYAESAVQVWRDEYKAFM
                     RGKRPFEGNYAETEGNLYYTAADNDGFLIATLDKADKTVAETLLAYPEKKEKTYYNVN
                     STDFLDGKLIISYSRYDEYSNDRGTDILIYDLDSGMQEEHFAFDIPADFTGYQSVTTS
                     VLTQGDQKAIFVMERLEETDSEATEYVAPVVSLSFKRYDWTTKEWSTLAAEEALVDDL
                     SYAIKDGIFYQLQHTDQEWSIQPLDLMQDQVRDKIILQNTADWEETVDMNWQILVSDS
                     RIILTENYLEPDRASQLAVFELQSGEMLYSGEINATFADTKKIDLLYFDKIIYEP"
     gene            complement(5768..6691)
                     /locus_tag="PLT73_01520"
     CDS             complement(5768..6691)
                     /locus_tag="PLT73_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM19093.1"
                     /translation="MKKINIGNSGLLASEISLGIMRMNALTHEEATVVIRTAVDNGID
                     YFDHADIYGRGTSEEIFGEAFKELGIARDSVMLQTKCGIVPGVMFDFSKEHIIASVEG
                     SLKRLQTDYVDALLLHRPDTLMEPEEVAEAFDALHKAGKVRHFGVSNQNPMQIELLKK
                     AVTQPLLINQLQLSLTHAPMIDAGFNVNMMNNPSFMHDGSVLEYSRISDMTIQPWSPF
                     QAGNGKKGLFFDHPDYPELNEKINTIAEDKGVSREAIAIAWLLRHPAKMQPIVGSMNP
                     ERIAAICKASDVALSRTEWYDLYKAAGKMLP"
     gene            complement(6832..7278)
                     /gene="nrdG"
                     /locus_tag="PLT73_01525"
                     /pseudo
     CDS             complement(6832..7278)
                     /gene="nrdG"
                     /locus_tag="PLT73_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
BASE COUNT         1977 a         1766 c         1707 g         2015 t
ORIGIN      
        1 cctgggttat ctgcttcgaa agcttctgcg atcgcttcca ttcctggttg ttggattttg
       61 tcccagatgc tataagtgat ggtcacatca ccggaaccgg cttctccatc ggaaccgttt
      121 ccgcatgcac ctaaaacaag tcctgataac aatactgtag ccaatgatac tttcttccaa
      181 ttctttaaca tctcttctcc accttttctt ttatctataa aattgtatac tcgaaaacca
      241 agaaactatt gggccataga gaaattgcac ctgaaccatt caaaatcaat cgttttgcaa
      301 acgtttactt gttttgatgt cataataata ccaaatacag gagagggctg atagatattc
      361 agccgtacaa aaacatacct ttttgttgat tttaaaaaaa tagcaataaa tactatactg
      421 gcaccgagct aaacagtaac ccttccggct ttcccactcc tttcgtctct ggtaagctga
      481 cttgttttaa attatagaca cttgaaagcg tatactatat agtgcaatgt tttaaatatc
      541 agtcaaaatg ttggatgctg aaagcacaaa gaaagccgga cctgcctgga atcgatgcaa
      601 tatcgttcca aacggcacgg cttttttatg ctacttgcct gattgcctca tttggttttt
      661 actttcttga cgtataccct tggggacagc tgcatcttgc tcttgaagat ccgcgtgaag
      721 ctcaaagggt tctgatagcc gaccttttcg gcgatttcag cgatgctgag gtccgtcgtt
      781 tccaagtatt cccggctttt ctggatgcgg aaatcgatca gatactcatg cggtgtccgg
      841 tttgtcgttt tatggaaaac ggcggtcaaa taggagtcgt tcagtgataa ttccctactg
      901 atgtcgccga tcagaaagtc actgtcctga tacctgtctt tgatgatttc gattgccctt
      961 ttcgtgtatt cgacaccttt attgattttc tcaatctgtt tgatttcggt gccatccaaa
     1021 tcatgtgcga gcatgctcaa aaagaggtac aactgtcctt gcagacgcag ccgggataac
     1081 gtatcgaacg gatttgccgc cataatctcc tgcagcatcg ccttcacctg cccaaagttc
     1141 ttcacgttcc ccaccggatg cgaaacctgg aaaccgatgg ccgtcagcaa agcttccaga
     1201 tgctcgccct tcattccgat ccaataatat tcccaaggat cctcttcatc cgcttgataa
     1261 taggtcatca cgttcggata gatgatgaag aactggcctt ctttcaggtt gaagcggcta
     1321 ttgccgattt cgaaatatcc ctttcccttc ataatgatat ggatcaaaaa atattcccgg
     1381 actgtcggcc cgaaactgtg gttgggagcg cactgctctt tgcctttggt tgtgtagtaa
     1441 agatccttgt aatagatttc ttccttaaaa aaatcttcat ccatttccat cacctcggtc
     1501 cttatttctg ttgtagccgc tgcccccatt atagaatgct ctccttgcta tggcaaaccc
     1561 gcacttttat ctgcaactct cctcccgcat gaaactccgc cctttcgcaa tcacagattc
     1621 tcttgtacaa tgagaaaaga ttgcccgcgt atcgggcaga tcccaaacaa actgactaat
     1681 cccatacctt tgctatagat ttggaggcaa tacccttgaa aaaaatcatc ctttccgcgt
     1741 tgctgcttct gccgctcttc ctgctttcag cctgcacaac cgccctgcct ggggaagaag
     1801 tcatgcagaa tctggtcaac cgttccgccg acctggacag ctatcaccag ttcgtcgctt
     1861 tcagaacaac aaccaccaaa gaggatctca atggagcaac aacagtgaac gagcagtaca
     1921 caaccgccga cgcgacgctg ttccctgaag aaaagaccgg ttacggaaag cggatagaca
     1981 aaaacagcga ttatcccgca accaaatccg aattctatgt gacccctgaa gtgggttacg
     2041 tccggaccga cgatgccgac tggaccgctt atgccacccc tgatgtaccc ttagccagcc
     2101 tgcaggtcta tccgctggaa acgttcatcg aactgagcca aatcattgaa acgtacggca
     2161 cgctcgaacg gaaaggggat gagtatgtgc tgcacttttc cggcagcgat gatgcgttga
     2221 acaaagagat caactaccta ttccagactt ttccagccga acggaccgat tacgatatcg
     2281 aaattcgtct ggatgccgaa accttctatc tgagcgactt ctcctaccgc tcgatcgtta
     2341 cgcaagccga ccagaaagcc gtctccacga cggaactgac cggcgagttc gatctgtatg
     2401 atcaagcaaa aaagttgaag tcggtgccgg tattcgattc agcaagtagt gtcccggaga
     2461 cgagtgattc ttcattttga taaaaattcg atcccaaagt caatcaatgt ctgcctctgg
     2521 gattctggtt gacttacaag tgtattatgc atataataca cttgtacata tattttttaa
     2581 cccaagtgta ttaaccgttt aatacaatcg atcggaaaag gcaggtggaa aaagtgattt
     2641 ttgacaaaag aagtccggtc tacgaacaga ttatcgaaat gcacaagcag aaaatcgtaa
     2701 gcggcgcttt ccaacccgga caagaaatac cgtcgcgccg ggaattggct acacaattaa
     2761 aagtgaatcc gaatacggtg cagcgtgctt ataaggaaat ggaggggctt ggtttgattt
     2821 atacagatgg aaattctctc agcaaaataa cggaaagcca atcaaaaatc cagacattaa
     2881 gacaagagct attggacgag gccttacagg gattcatcga aacggtacgg acaatcggac
     2941 tggaggatga agcggtcctg gacttgatca aaaagcgctt gaaggaggtg cacgtgaatg
     3001 attgaaatca agaatctgga aaaaaaatac ggcaaacgga ccattttgga agatgtcagc
     3061 tttaccatca aaaaaaatga agtgacttgt ctgaccggaa tgaacggtac aggcaaaacg
     3121 accctcatga actgtattat gggcttagtt ccacgcaaca acggctcgat tacgatcgac
     3181 gggcaagaca ttcataaaca gctatacgaa aaaatctcct atatccctga ctcactgacg
     3241 atgccccgat ggatgacaat cgcggagtcg atgaagttta tgagtaccta ctacccgaat
     3301 tggaatgccg ccaaagcgga tgaattactt tcgtttttcc gtctggaatc ggatctgcaa
     3361 ctgaaaaatc tctccaaagg gaacagcgca aaggtgagtt tcttgttggg actttctctg
     3421 gacgcggatt actatttgat ggatgagcca ttttccggca tcgatgtctt tgcccgcgag
     3481 gatattctcg aggtcttcac gagcaaattc gtgacggaca agggcgtcct catcgccact
     3541 catcatatgg atgaagtcga aatgttgttg gaccggatcg tcatgctgcg cagcgggcgt
     3601 gtcgtcaaag atttttacgc agaagacatc cgccaaaacg aagggaaggc tattattgat
     3661 gtgatgcggg aggtgtatca accatgaaaa aatttatgga actatggata tttgaatgga
     3721 atcgcgcgaa acggttctat ggcttcctgt tggcttttgt gacggcttta caggcttatg
     3781 cagtcttcca cgaataccat atctggaaag acggatatga gaactaccgg caacagaact
     3841 atgctgcact acccgaacaa tatctgcagg actatgggta tcttacgttg gaggatgtga
     3901 caaacaactc gttcttcgtt ttttcgatca tgacagccat tttcgcattg ctgttctatg
     3961 ccgtctttat ctggtatcag gattggtctg gaaaaaaccg tttctctttc cgtttgctca
     4021 gtctgccggg taaccgtttc gctgtgtatg cagcaaaatt cgcgaccatt tggctcctga
     4081 ttacagggct gcaagtttgg caaatcgggc ttttgtatct tgaggagctg gtcttttccg
     4141 gacttattcc ggcggacatc tatcgggaaa tccccttgca gatggcatca accagcgctt
     4201 ctccattggc cttggcttta ccaagtctct tttccaatta tctgcttttt tacactattg
     4261 gctatacagc gctcacttta gggtacacgt tcatcctgat gcacttgagc ggacattgga
     4321 aaggggtatt cttggcagct actcccgcaa tcgtcctctt tgccgcgatg cttctcttct
     4381 tgcgtaccgg catcacgacg cgtttgtatg cgcaagaaaa gtactggatg acgattgccg
     4441 tgagccttat gcttttgctg tccgggacat gggctaatta tcggttgctt gaaaaacgca
     4501 taagtgtata gaaggaggac aaaaaatgag aaaacaaacg attttattgc tgagtctcct
     4561 cctgcttacc gttctcggag ggttctatgt ccagccgcta ttggcaaaag aagagccttt
     4621 gcgcatcggt attgagacag tttctggtga tgaagcagtt ttggatggct tactctatta
     4681 cggaacagca cactcgcaat gggaaacgac tacactaagc tttgacagct ccggcttgcg
     4741 ccattccgaa acgcaacgtc aggatgcatt cttccaagac tacgcagaaa gcgctgttca
     4801 ggtttggcgc gatgagtata aagcatttat gcgtgggaag cggccttttg aagggaatta
     4861 tgccgaaacc gaaggaaact tatactatac agcagcggat aatgacggat tcctgatagc
     4921 gacactggat aaggctgaca aaactgttgc ggaaacgctc ctcgcttatc cggaaaaaaa
     4981 agaaaagacc tattataacg tcaatagcac ggacttcctg gacggtaagc tgattatctc
     5041 gtacagccgt tatgatgagt attcaaatga tcgtggcacg gatatcctga tttatgatct
     5101 ggacagtggt atgcaggagg aacattttgc tttcgatata cctgcggatt ttacgggtta
     5161 tcagagtgtt accacatccg tgttgacaca aggcgatcaa aaagcaatct tcgtcatgga
     5221 gcggctcgaa gaaactgatt cggaagctac agaatacgtt gcaccggttg tctcactatc
     5281 cttcaagcgc tatgattgga caacgaagga atggtcaaca ctggctgctg aagaagcatt
     5341 ggtggatgat ttatcttacg caattaagga tggtattttc tatcaattgc agcacacgga
     5401 tcaggaatgg tcaatccaac ctcttgatct gatgcaggat caagtgcgcg ataaaatcat
     5461 tctccagaat actgctgatt gggaagagac cgttgatatg aattggcaaa tcctcgtttc
     5521 cgacagccgc atcatactga cggaaaatta tctggagcct gaccgggctt ctcaactggc
     5581 tgttttcgaa ctccagtccg gggaaatgct ctacagcggg gaaatcaatg ccacgttcgc
     5641 cgatacaaaa aaaatcgatt tgctctattt tgataagatt atttacgaac cctaaagcga
     5701 aaaaagaaca agattccaat tggagtcttg ttctttttta tttctcttta actgttgctg
     5761 cctttcttta aggcaacatt ttgcctgcag ccttgtagag gtcataccac tcggtgcggc
     5821 tcagcgccac atcggaagct ttgcagatgg ctgcaatacg ctctgggttc atgctgccga
     5881 cgattggctg cattttagcc ggatgacgca acaaccacgc gattgcgatt gcttcccggg
     5941 atacgccttt atcttccgca attgtgttga ttttttcatt caattccgga taatcgggat
     6001 ggtcgaagaa cagccctttc ttgccattac cagcttggaa tggcgaccac ggctggatcg
     6061 tcatgtccga gatccggctg tattccagga cggaaccgtc gtgcatgaat gaaggattgt
     6121 tcatcatgtt cacgttgaat cctgcatcga tcatcggtgc gtgggtcagg ctgagctgca
     6181 gttgattgat caataatggc tgtgtgacag ctttcttcag cagttcgatc tgcatcggat
     6241 tttggttgct gacaccgaaa tggcgcactt tccctgcttt gtgcaacgca tcaaaggctt
     6301 ccgcaacttc ctccggttcc atcaacgtat ccggccggtg caaaagcaaa gcatcgacat
     6361 aatccgtctg gagacgtttc aggctacctt ccaccgaagc gatgatatgt tcttttgaga
     6421 aatcgaacat gacacccggc acgatgccgc atttcgtctg cagcatcacg ctgtccctcg
     6481 cgatgccgag ttccttaaag gcttctccga aaatttcctc cgaagtccct ctgccgtaga
     6541 tgtccgcatg atcgaagtaa tcgatgccgt tgtccactgc cgtgcggatg accactgtag
     6601 cctcttcatg cgtcaaagcg ttcatacgca tgatgccgag cgaaatttct gatgctaaca
     6661 gtcctgaatt accgatgttt atttttttca attgtcatac caactttctt attgggatta
     6721 tttgaaaacg tttcaataaa attagtatag catttttgat gtgtttttgg gtaatgaagc
     6781 gcagcaaaaa aatgctgcct cgaagtgagg cagcacgata tgttttaaca ttcaagcgta
     6841 agcattggcc caaagaactt tttctccggc ttccagcgat ttttgcacat cgatgatagt
     6901 ctgattggag ctgccgcgga atttcaatgt cagatcccgt ttggacagtt cgaagcggcc
     6961 gtcgacgagt acatccatca agcgcagcaa ttcctgcttg tcttcggatt ctgaagccaa
     7021 ttcgtcccaa tagtagccgg tccagcccca gatgtccttg gtccggccga actccgcacg
     7081 gatgcgtcgc gcaagccgca gcgcaacctt cgtgttgagg aacggttcgc cgcccaaaag
     7141 cgtcaagccc tgcacataag gctggcgcag atcggccatg atctgttcct ccagctcggt
     7201 cgtgtaggca atcccgtagt cgaaattttg gacaatctta ttgtagcagc cttcgcaggc
     7261 gaacaggcag ccgctcagct atgttgttgt gaaatcgcag gaaaaggtaa aagcagtgtt
     7321 tgaacatacg aaatgggcag gttatcttcc gaaagaggaa gggcagccga aagccgggga
     7381 agaaccggtc ttattcatag gaattataga aaatgagaat attaaccggg ggcaatattg
     7441 tgatacagat gccggactgg caatc
//
LOCUS       DAOMUJ010000030         7424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2614_length_7424_cov_1.986023, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000030 DAOMUJ010000000
VERSION     DAOMUJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_7424_cov_1.986023"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLT73_01530"
     CDS             complement(<1..1332)
                     /locus_tag="PLT73_01530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM19094.1"
                     /translation="MMMMEEMAMPFEKYWHEMSFQLPTPIQENVFKPLLQGKDVVGLS
                     PTGTGKTLAYAIPLLQKTDANKELQLLVLVPSQELAHQVGHVLAEWGSLKDLSAQVII
                     GGANVGRQIDKLKEKPEIVVGTPGRLLELANQKKLKLHNIKTVILDEADYLMQEEHLS
                     ALRELIKKMPGSRQMGFFSATNSENLKDIQKWFNTEPLWFDTTKEGQFMDQTIHGFIE
                     APTRKRAEMLRRLGNLEEMQALVFVNATQELDYLAQKLQFEGINVRMLHSDYGKAQRK
                     NAMDEFRKGNVTFLLTTDVSARGIDIQDLPYVIHYDLPLSKETYLHRSGRTGRMGKKG
                     IVLSLVNDRNSRDIKRFAPHPEEVKEFFIYGGELVDQLPSRESRDAIRDAQKPAVAKA
                     AKIQKKAALVASKEEKAKKEAVAKKTKKKKNEKERTYFKIKNLEFNFFFLIH"
     gene            complement(1390..2396)
                     /locus_tag="PLT73_01535"
                     /pseudo
     CDS             complement(1390..2396)
                     /locus_tag="PLT73_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(2537..3238)
                     /locus_tag="PLT73_01540"
     CDS             complement(2537..3238)
                     /locus_tag="PLT73_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19095.1"
                     /translation="MNFYYDEEAIRCLKAKDPILADVIDRIGPIERPIDADLFSALVN
                     NIVGQQISMKAQETVWKRMIAVFGAITPDRICRASIEEIQQCGLSIRKATYIREAAEK
                     VISGACDLEALKDMSDEAVIAELSQLRGIGKWTAEMLLIFSMGRQDVLSWDDLAIHRG
                     LRMVYHHRKITKQLFQKYKRRFAPYGSVASLYLWEVSVGTLPDLKDYAPLTEAEKRKR
                     LKQRQELKKAEKQQS"
     gene            complement(3337..4215)
                     /locus_tag="PLT73_01545"
     CDS             complement(3337..4215)
                     /locus_tag="PLT73_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM19096.1"
                     /translation="MVKSLMDRMPLNDGFTIPGIGYGTWNVSNDEAEEMVFMAIMRGY
                     RLIDTGSMYGNEVGVGRGIHKAINAGISRDDIFVVTKLGKDDMGFEKATDAIRASYDR
                     LGLKYIDLFLIHFPSPNEGVTAATWKALEEAKDSGIVRSIGVSNFMRGDLKELLENSR
                     IKPVVNQLEINPFNLMEEVDDFGYDNNIMTMAYSPLAKGRALEDGKVMKIAEKYNKTP
                     AQIILRWAVQRDLIPIPKTKDADRMKENLDLFDFSLTDEELERITRIKKDSNFVKGHQ
                     DHNLGKRIGVDIRNSK"
     gene            complement(4355..5236)
                     /locus_tag="PLT73_01550"
     CDS             complement(4355..5236)
                     /locus_tag="PLT73_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM19097.1"
                     /translation="MNIKNKSLLKTAVLAGTAAYLHLQNTWIQKTEYTIPVKNLAPEN
                     EGLKIVQLSDIHLPKEKVDLNKLIDMTKAADPDFIFLTGDQIQGDGTFDPSVAEKLFR
                     KLTAIAPTYAITGNHDRHSPMFTIVLDLYKQTGIRFLDDEAISVLAKGRKPVVIMGLS
                     DKSSAIQKIAKDFLKKIRLRKDWQGQTRLLLAHRPEHFLKYHIDPEKSADITFAGHAH
                     GGQIRIPRLGGLYAPGQGRMPRHTEGVYLLPTDPEKKLVVSRGIGGSSFPFRINNRPE
                     MVVVTLTNGSTSESLEI"
     assembly_gap    5548..5557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5558..>6175
                     /locus_tag="PLT73_01555"
     CDS             <5558..>6175
                     /locus_tag="PLT73_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HRM19098.1"
                     /translation="VVLLHFTLTPLETIDLQRFLFGAFLIIIGLSIFLFGVDLGITPI
                     GKFLGKGLARSNSMKYVLGMGLVLGFFISIAEPDLHILATQVSEVTSGMYPKNLLLIV
                     VSVGIAVMLTIGLFRIVYRYPLNKTFTFLYLAIFGLAYFSTNDLFAIAFDASGSTTGA
                     LTVPFMLALAVGVASLNRKTQSAEEDSFGLVGIASAGAILAVLILG"
     assembly_gap    6176..6219
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6220..6927
                     /locus_tag="PLT73_01560"
     CDS             <6220..6927
                     /locus_tag="PLT73_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HRM19099.1"
                     /translation="IAGEILLAVSPILIIFVLNHVFFADQKLSKRAFRRIFLGMAYLF
                     VGLVLFLTGVNAGFMEVGRKLGMLIAGMDSSIPVLIVGFVLGVLVILAEPAVYVLTHQ
                     IEDVTTGYVKRGIVLGFLSVGVGLAVLLSVVRVLVPWLQLWHFLVPGYLIILALSYKV
                     PKLFVGIAFDAGGVASGPMTATFILAFIQGVAEITPDANVLLEGFGMIAMVAMMPIIS
                     LQLLGAIYQRNSIKEGL"
     gene            6928..>7424
                     /locus_tag="PLT73_01565"
     CDS             6928..>7424
                     /locus_tag="PLT73_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19100.1"
                     /translation="MTEQVENKRFYDVIFVVVNIGKASRVLEEAKKCGITGGTILHGS
                     GTVSSALLRTIGFYEVKKEILFMVCARDKTQPTVEHISSLFHFELPNRGILFTSPISH
                     LLGTHGERLHMDEKDPHPTSGHEVFFTLVAGGLGDDVVEAAAEAGARGGTLFHGTGAF
                     ADKCR"
BASE COUNT         1610 a         1899 c         1772 g         2089 t
ORIGIN      
        1 atgaatcaaa aaaaaaaaat taaattccaa atttttgatt ttaaaatatg tcctctcttt
       61 ttcgtttttt ttctttttcg tttttttggc gactgcttct ttcttagcct tttcctcttt
      121 cgaagcaacc agggcagctt ttttctggat tttagcagct ttggcgacgg ccggtttctg
      181 cgcatcgcgg atggcgtcgc gggattcgcg gctcggcaac tgatcaacga gctcaccacc
      241 gtagatgaag aattccttca cttcttccgg atgcggggcg aaacgtttga tatcgcggct
      301 gttgcgatca ttcaccaatg acagcacaat cccttttttg cccatccggc ccgtacgtcc
      361 ggagcggtgc aggtaggttt ccttcgatag cggcagatcg taatggatga cgtaaggcag
      421 atcctggatg tcgatcccgc gcgcggaaac gtccgttgtc agcagaaagg tcacattgcc
      481 tttgcggaat tcatccatgg cgtttttgcg ctgggctttg ccgtaatcgc tgtgcagcat
      541 gcggacattg atgccttcga actgcagttt ctgcgccaaa taatcaagtt cctgcgtagc
      601 atttacgaaa acgagcgcct gcatctcttc cagattgccc aaacggcgca acatttcggc
      661 acgtttgcgc gtaggtgcct cgatgaagcc gtgaatcgtt tggtccatga actggccttc
      721 ttttgtcgtg tcgaaccaaa gcggctcggt gttgaaccac ttttggatgt ccttcaggtt
      781 ttcgctgttc gtagccgaaa agaaacccat ctgacgggaa cccggcattt ttttgatcaa
      841 ctcgcgcagg gcgctcaagt gctcctcctg catcagatag tccgcttcat caaggatgac
      901 cgtcttgatg ttgtgcagtt tcagtttttt ctggttggcc aattccaaca atcgtcccgg
      961 tgttccgacc acgatttccg gtttttcctt cagcttgtcg atctgacggc cgacgttcgc
     1021 gccgccgatg atcacttgcg cgctcaagtc cttcagggaa ccccactccg ccaaaacgtg
     1081 gcctacttgg tgagccaact cctgcgaagg cacaagcacc aacaattgca gctctttgtt
     1141 ggcatccgtt ttctgcagca acggaatggc ataagccaac gttttacccg ttcctgtcgg
     1201 cgatagaccg acgacatcct tgccttgcag caacggcttg aagacattct cttggattgg
     1261 ggtcggcagc tgaaaactca tctcatgcca gtatttttcg aatggcattg ccatttcttc
     1321 catcatcatc attcatttcc tcatttctta tactgcggcg catacctgtt gcaggtacaa
     1381 caatctgtct tacgcctcgt ccagatccgc tgggtaaacg atgcccgctt ttttgcgcaa
     1441 ggcctccatc gtccggttaa cgtcccggct gatctgcgtc caacgttcgt agtcttctat
     1501 agtatcagga ttttccggat cattcaagac gcgcgcgaag gcacggactt cgtcgtccat
     1561 gaaatggccg gttgcttttt cggccaattg ataccgttca tcagtctcgc cgtccaccag
     1621 ttcgaccgat tggatcaaat tgacaccatc gagaagcaag gtttttttgc cgctgtagat
     1681 ttccgaatcg gcgaaggaat tgtagatttt cgacgtatgc aaggtgacgg agaaatacgg
     1741 atatttcaac agcatcgtac cggccccgtc cacgcccgtc tggattttct tgtcggcata
     1801 atcgacgtca tcaggcacac cgaaccaagc gatagcagcg tacagcaaat atacgcccaa
     1861 atctgcaagc gccccgccgg aaaaagcgag cgagaaaata ttcggttctt cgccggctaa
     1921 gacttggtca tagcgggaag agtattttgc ataggtgaag ttgcaccgtc gatttctccc
     1981 aatttggcga tttcattccg gaccagctcg aagtttggct catgcagatg gcgagcggct
     2041 tcaaacagga agacattatt ggctttggcc gccgagaagg cagcttccca ttcttttgta
     2101 ttcgagaaga tcggcttttc gacgatgaga tgcttgccgt tggcgatggc cgacatggct
     2161 tgctggaaat ggaggctgtt cggtgatgcg atgtagatgg catcgacttc ctccaaggca
     2221 gtgaattttt ccaagtccgt gacgaaaata tccgcaccgt agccttctcc aaagctttgg
     2281 gcggtgaact ggttgcggct gtagaccgct tttatttcga attgtccgga tgctttggcc
     2341 gctgttgcga atgcatgcgc aatgctgctt gttccgatga tgcctaatct gatcatggtt
     2401 tgatcccctc actcattgtt tttttgaaac actctattcc cattctacac taatcgaagg
     2461 tggattgctt attgatagcc attattttga acccaaaaac agcatcccgg cacaaaccgg
     2521 atgctggcgg cgaagattat gactgttgct tttccgcttt tttcagttct tggcgctgct
     2581 tcaggcgttt tcgcttttcg gcttccgtca gcggcgcgta gtccttcaga tccggcagag
     2641 tcccgacgga tacttcccag agatagagac tcgcgaccga accgtatggc gcaaaacgcc
     2701 gtttgtattt ctggaacagt tgcttggtga ttttgcggtg atgataaacc atccgcaagc
     2761 cgcgatggat agcgaggtca tcccagctca gtacatcctg acggcccatc gaaaagatca
     2821 gcagcatctc ggctgtccat ttaccgatgc cgcgcagctg cgacaactcg gcgataaccg
     2881 cttcgtcact catatccttc agcgcctcca gatcacaggc accggaaatg actttctcgg
     2941 cagcctcacg gatataggtt gctttacgga tcgataggcc acactgctgt atttcttcga
     3001 tgctcgcccg gcagatgcga tccggtgtta tcgccccaaa aacggcaatc atccttttcc
     3061 agacggtttc ctgagctttc attgaaatct gctggccgac gatattgttg acgagcgcgg
     3121 aaaaaagatc cgcatcgatc ggccgctcga tcggtccgat ccggtcgatg acgtccgcca
     3181 gaatcggatc cttggccttc agacagcgga tcgcttcttc atcataataa aaattcattt
     3241 actccaccct tgctctgcat atttttcaac cataacccct ttcccccata agaaaagctg
     3301 gagactcgga agcccccagc tttgatttga ttggcatcac ttactgttac ggatgtcgac
     3361 cccgattctc ttaccgagat tgtggtcttg gtgccccttc acgaagttgc tgtctttctt
     3421 gatgcgggtg attctttcta attcttcatc cgtcaaacta aaatcgaaca gatcaagatt
     3481 ttccttcatt cggtcggcat cctttgtttt cggaatcgga atgaggtcgc gttgtacggc
     3541 ccatctcagt atgatctgtg ccggtgtttt attatatttt tcagctattt tcataacttt
     3601 gccgtcttcc aaagctcttc ccttagccaa tgggctgtag gccatcgtca taatgttatt
     3661 gtcgtagccg aagtcatcca cttcctccat gaggttgaat ggattgattt ccaattggtt
     3721 cacgactggt ttgatgcggg agttctccaa tagttctttc agatccccac gcatgaagtt
     3781 ggagacgccg atgctgcgga cgattccgga atccttggct tcctccaagg ctttccaagt
     3841 ggctgcagtt acaccttcat tagggctcgg gaagtggatc aggaacagat caatgtactt
     3901 tagtccgagc cggtcgtagc ttgcacggat agcgtctgtc gctttttcga agcccatgtc
     3961 gtctttaccg agtttggtca cgacaaaaat atcatctctg gaaatgcctg cgttgatggc
     4021 tttatggata ccgcggccta cgccgacttc gtttccgtac atgctgcctg tatcaatcag
     4081 gcgataccca cgcatgatgg ccatgaagac catctcttca gcttcatcat tgctcacgtt
     4141 ccaagttccg tatccgattc cggggattgt aaaaccatca ttcaaaggca ttctgtccat
     4201 taagctcttc accatgataa ctcacactcc ttctacttat taccttaagt gtaacgcaat
     4261 ttcctcagaa acacaaaaat tattaatgcg cttacaaaac aaaacggagg aaaaaaactt
     4321 tcctttcctc cccgaaccaa tccaatccaa acggtcatat ttccaaagat tctgaagtgg
     4381 agccgttcgt taaggtcaca acgaccattt ccggtcggtt gttgatccgg aacgggaatg
     4441 aggacccacc gatgccccgg ctaacgacca gtttcttttc cggatccgta ggcaacagat
     4501 aaacgccttc ggtatgccgt ggcatccgcc cttgtccagg agcatacagc ccacccaatc
     4561 tcgggatgcg gatctggccg ccatgagcat ggccagcgaa ggtgatatca gctgatttct
     4621 ccggatcgat atggtacttc agaaaatgtt caggtcggtg cgccaacaaa agacgtgttt
     4681 ggccttgcca atcttttctc agcctaatct ttttcagaaa gtccttcgcg attttctgaa
     4741 tggccgagct tttgtcgctc agtcccatga tcacgacagg cttgcgaccc tttgcaagga
     4801 cgctgattgc ttcatcgtcc agaaaacgga tgcctgtctg cttgtacaaa tccaacacga
     4861 tagtgaacat cggagaatgg cggtcatgat tacctgttat ggcataggtc ggtgcgatcg
     4921 ccgtcagttt ccggaacagt ttttccgcga ctgaaggatc aaaagtacca tccccttgaa
     4981 tctgatcacc agtaagaaag ataaagtccg gatccgctgc tttggtcatg tcaatcagct
     5041 tgttcagatc gactttttct ttcggcaaat ggatatcgga aagctggact atcttcagcc
     5101 cttcgttttc aggcgccaga ttctttaccg gaatggtgta ctcggtcttc tggatccagg
     5161 tgttttggag atgcagatag gccgctgtgc ccgccaaaac ggcagttttc agcaggcttt
     5221 tgttttttat gttcatattt atcatcctct atcttcaaga aaccctattg tttcctctat
     5281 tctaatgctt tggagcgctt cagtcaaaat aaaacggttg accgtcggaa gcgattatcg
     5341 gagctgggta attttcctga aaccataagg atgttgtccg atacggttca aataggtgct
     5401 ttttgcttcc tattaaataa ttagcatgtt atgtttaata tatgaatatt actcctcaac
     5461 attacaacat cactacgaag ggactgaatc tatgagtata tttacagaaa aattaaggga
     5521 agtcctgcag tccgtgctgc cgatcacnnn nnnnnnntgt tgtgctgctc catttcacct
     5581 tgactccgct cgaaaccatt gatctgcagc gtttcctgtt cggggcattc ttgatcatca
     5641 tcggcctgtc gatcttcctt ttcggtgtgg atctgggcat cacaccgatc gggaaattcc
     5701 tcggaaaagg gttggcccgc agcaacagca tgaaatatgt acttgggatg gggcttgtgc
     5761 taggattctt catctcgatt gcggagccgg atctgcatat tctggcgacg caggtttccg
     5821 aggtcacaag cggcatgtat ccgaaaaacc tgcttttgat cgtcgtttcc gttggcatcg
     5881 ccgttatgct gacaatcggt cttttccgga ttgtttaccg ttatccactg aacaagacgt
     5941 tcactttcct ctacctggcc atcttcggct tggcctactt cagcaccaac gacctgttcg
     6001 ctatcgcttt tgatgcttcc ggatcgacga ccggcgcttt gacagtgccc ttcatgttgg
     6061 ctctggcagt cggcgtcgca tccctgaacc ggaagacgca atcagccgag gaggatagtt
     6121 tcggtctggt agggatcgcc tcagccggtg ccatcctggc cgtattgatt ttgggnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tagcgggcga aatcctgttg
     6241 gccgtttcac cgattctgat cattttcgtg ctcaaccatg tcttttttgc tgatcagaaa
     6301 ctctccaaac gtgctttccg ccgtattttc ttgggaatgg cctatctgtt cgtcggtctg
     6361 gtgcttttcc tgactggcgt gaatgccggc ttcatggaag tcggccgcaa actcggcatg
     6421 ctgattgccg ggatggacag ctcgattccg gtcctgatcg tcggttttgt gctgggggta
     6481 ttggtcattt tggcggaacc tgcagtatat gtattgacgc atcaaataga agatgttaca
     6541 acagggtatg taaagcgcgg tattgtgctg ggattcctgt ccgtaggcgt cggcctggcc
     6601 gtcttgttgt cggtcgtgcg cgtgctcgta ccatggttgc aactgtggca ctttctcgtc
     6661 cccggttatc tgattattct cgccctctcc tataaagttc cgaagctgtt cgtcgggatt
     6721 gcctttgatg cgggcggcgt ggcttcaggc ccgatgacgg cgactttcat tctggcattc
     6781 attcagggtg ttgcggaaat cacaccggat gccaacgtgc tcttggaagg cttcgggatg
     6841 attgcgatgg tggcgatgat gccgatcatt tcgttgcaat tgttgggcgc catttatcag
     6901 cgcaacagta taaaggaggg tctctaattg accgaacaag ttgaaaacaa aagattctac
     6961 gatgtcattt ttgtcgtcgt caacatcggc aaagccagcc gcgtgcttga agaagcgaaa
     7021 aaatgcggca tcaccggagg gaccatcctg catggttccg gaacggtttc cagcgcttta
     7081 ttgcgcacaa tcggttttta tgaagtaaaa aaagaaatcc tctttatggt ctgcgcgcgc
     7141 gataagacgc aacctactgt cgaacacatc agctcacttt ttcatttcga gttgccgaat
     7201 cgtggcatcc tcttcacaag tccgatttcg cacttgctgg gtacgcatgg cgaacgcctg
     7261 catatggatg aaaaagatcc ccatccaact tccggtcatg aagtcttctt tacgctcgtt
     7321 gccggcggat tgggtgacga tgtcgttgaa gcagcggctg aggctggcgc acgcggcggc
     7381 acccttttcc acgggactgg cgcttttgcg gataaatgcc gaaa
//
LOCUS       DAOMUJ010000031         7035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2835_length_7035_cov_3.015759, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000031 DAOMUJ010000000
VERSION     DAOMUJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_7035_cov_3.015759"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLT73_01570"
     CDS             complement(<1..1036)
                     /locus_tag="PLT73_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRM19101.1"
                     /translation="MAATVQSKSQVHAAESDEPITFSNASVHDPSIIQDQKSGMYYVF
                     GSHIAAAKSPDLINWQNFTNGYTSTDNTIYGDLSGNLSESFAWAGEDDADSKGGYAVW
                     APDVIWNKQYRNADGSKGAYMLYYSVSSTYIRSAIGLAVSKNIEGPYTYVDTIIYSGF
                     TEEETYDANSDVNKQWESTNIDELIEEGVLEEPNPEWFTENGVYNNAEYTNAIDANVL
                     YDKQGKLWMTYGSWSGGTFILELDKKTGIPLYPGEDGVTKDGRMIDRYFGTKIAAGYG
                     RSSEGTYAVYDKKAGYYYLYLTYGGLASDGGYQMRQFRSKSITGPYVDAAGSEAVYPE
                     TFDKGVGNFPG"
     gene            complement(1447..1785)
                     /locus_tag="PLT73_01575"
     CDS             complement(1447..1785)
                     /locus_tag="PLT73_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRM19102.1"
                     /translation="MIQYQEKEFYNTKDLALSVVGGRWKIAIIWSLLHNGTLRLSEFN
                     KILPDINQRMLIRQLRELERDLIIARTVYPVVPPKVEYHLTDIGKELAPVVQSICDWG
                     DQYLKVITEE"
     gene            1909..3024
                     /locus_tag="PLT73_01580"
     CDS             1909..3024
                     /locus_tag="PLT73_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRM19103.1"
                     /translation="MKSRAAVAFGPGKPLEIVEIDVAEPKAKEVLVKILYTSVCHTDA
                     FTLSGEDPEGVFPAVLGHEGAGVVVAVGDEVTSVKPGDHVIPLYTPECGECEFCLSGK
                     TNLCSAVRETQGKGLMPDGTTRFSYNGEPIYHYMGTSTFSEYTVVNEINLVNIDKEAP
                     LDKVALFGCGVTTGLGAVENTAKVEEGAVTAVFGLGAIGLAVIQGLKKAKAKRIIAID
                     MNPDKWELAKKMGATDFVNPSEHDRPIQEVLIEMTNGGVDYSFECIGNVEVMKAALEA
                     CHKGWGESIIIGVAGAGKEIHTRPFQLVTGRVWRGSAFGGVKGRTELPGMVEDFMNGE
                     IDLDSFITHHLNFTDINEAFDLLHKGESIRTILTYGE"
     gene            3029..3859
                     /gene="fghA"
                     /locus_tag="PLT73_01585"
     CDS             3029..3859
                     /gene="fghA"
                     /locus_tag="PLT73_01585"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRM19104.1"
                     /translation="MTTTLIETHKVFDGEQRKYRHYSETLDCDMVFSLFLPQQTDNKP
                     TDLIWWLSGLTCTDDNFSQKGAFQKYANQHHMAVVMPDTSPRGTAVADDEGWDLGQGA
                     GFYLNATQEPWAKNYRMYAYLTEELPQLVYALIPNFSGKESIMGHSMGGHGALVIGLK
                     NPERFRSISAFAPILNPSQVPWGQKAFTAYLGTDETNWAEWDATSLIAESPAAPPILI
                     SQGTADNFYDVQLNEEAFLQAAEGKKVRYEKLDGYDHSYFTIATFIEDHLQFHKDCLK
                     "
     gene            complement(3958..4914)
                     /locus_tag="PLT73_01590"
     CDS             complement(3958..4914)
                     /locus_tag="PLT73_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19105.1"
                     /translation="MKETEKVLKQLNKFILNHVEDYDSVDEAVEAFTKLSNEASAKGE
                     TFVIGEMTNEEKATEELEKLEFAASEREYAAILERALKLDPDNLEARLFKLDPESLEF
                     IHELKKLETLGKAVMQKRGLDDEESIGNYWGIVETRTYLRIKALYADELQKRRMLTAS
                     VREYEDILRLNEADNMGIRYILMGIYCQLEQIEKAKELYQRFPEASAQMLLPLILLSL
                     KYDDELSAKRYYKDLQKENKSCKKVFGRREFDLESMVEAIDAVDYLADSEEELYIAIS
                     NVMTDFAVVPDMYYFNWLKKNLIKPPSSKPSKPSKKWIQKKK"
     gene            complement(4952..6244)
                     /gene="pepT"
                     /locus_tag="PLT73_01595"
     CDS             complement(4952..6244)
                     /gene="pepT"
                     /locus_tag="PLT73_01595"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM19106.1"
                     /translation="MYENLLERFIAYAKVDTRSDDSSYTVPSTHNQVTFALELAKELI
                     RIGLEEVEYNETNGFLTATLPSNTAEAVPVIGFIAHLDTADFNSVNIHPQVHCAYDGG
                     DIVLNEAEKIILSPKEFPRLRNYIGETVITTDGTTLLGADDKAGIASIVTACEQFIAH
                     PERKHGKIRLAFGPDEELGARGAKLFDVDRFAADFAYTIDGGPIGNLTYDNFNAAQAE
                     LTIRGTSVHPGSAKDTMVNALLVASQFATALPQEETPERTSGYEGYYFLTSQSGTVDE
                     VKQTYYIRDHDRERFAARKRFFTEQVAAFNSQFVEPRISLTLFDQYYNMRDIIEQHPI
                     VVDLAVQAYESCGITPVFKPMRGGTDGCIISHKGLPTPNLFSGTENGHGKYEFVTLET
                     MAKSVDVIMAIVEQSAVLSKTFQLEGIFSLTQAVKNRV"
     gene            complement(6375..>7035)
                     /locus_tag="PLT73_01600"
     CDS             complement(6375..>7035)
                     /locus_tag="PLT73_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19107.1"
                     /translation="VLNDGSVPSNGFVPSNYAFNPETDEDFREESGDLAVYDLETAQA
                     EWETAKEELGIETLSIELLTSDTETSKTTAEFLQSQWQQNLPGLTITIRNVPLKSRME
                     STTNGDYDIAYGTYTPSYADPIAFLEMYESTSGLNSSRFADEGYDALLDDTRSTYAND
                     PEQRWETLLAAEETLIAENAVNAPIYQGANANLVDPSLKDVQIQPVGAAMYFRTAYVE
                     E"
BASE COUNT         1679 a         1797 c         1701 g         1858 t
ORIGIN      
        1 ccccggggaa attgcctaca cctttgtcga aggtttcggg atagacggct tcgcttcctg
       61 ctgcatccac gtaaggtcct gtgatcgact tcgaacggaa ttgtcgcatc tggtagccgc
      121 cgtcagacgc caatccacca tatgtcaaat aaagataata atagcctgct tttttgtcgt
      181 agacggcata agtcccttca gaagaacgcc catacccggc ggcgatcttg gttccgaaat
      241 aacggtcgat cattcggccg tccttagtca cgccgtcttc gcccgggtaa agcggaatgc
      301 ccgtcttttt atccaattca agaatgaagg tcccgcccga ccacgaacca tacgtcatcc
      361 acagtttccc ttgcttgtcg tacaggacat tggcatcgat ggcgttcgtg tattccgcat
      421 tgttgtagac accgttttcc gtgaaccact ctggattagg ctcttccagt acgccctctt
      481 cgatcagctc atcgatattt gtgctctccc attgcttgtt gacgtcactg ttcgcatcat
      541 acgtttcttc ctccgtgaat ccggaataga tgatcgtatc gacatatgtg tagggtcctt
      601 cgatattctt ggacacagcc aagccgatag ccgaacgaat gtaagtagat gaaacgctgt
      661 agtacagcat gtaggcgcct ttgctaccgt ccgcatttct gtactgcttg ttccagatga
      721 catccggcgc ccagaccgca tagccgcctt tgctgtcagc atcatcttcc ccggcccaag
      781 cgaaggattc ggaaaggttt ccgctcaggt caccgtaaat cgtgttgtcc gtactggtgt
      841 agccgttggt gaaattctgc caattgatca gatccggcga tttcgcggct gcgatatgcg
      901 aaccgaatac gtaatacatc ccggatttct gatcttggat gatggaagga tcgtggaccg
      961 atgcattcga gaaagtgatg ggctcatctg attctgccgc gtgcacctgt gacttggact
     1021 ggaccgttgc agccatgctg atggccatac cgcaaagcaa caacttggat attcttgacc
     1081 tggtaatgat aaaaaacgcc tcctttataa ttgcaaccgc taacatttga ttccgcacaa
     1141 aatagatgta cttacaaatt aagatatcaa atttaatttg ttcgtacaac tattatgatg
     1201 gtcggagttt acttttttat tttgagcagg gtggcgggtt atttttcttt cgttttggct
     1261 gaaataaccc gccaacgggc ttttggtcgt ttattttgcg ttatattcgc cgagataagc
     1321 ggccaactgg tttttggcgg ggtactccac tcacctttcg atactaaaaa cccgccaaaa
     1381 agggtttggc gggtttttag tattcttgca gccttatact cttccggcta atgactcaat
     1441 cacagactat tcctccgtta ttactttcag atattggtct ccccagtcgc aaatggattg
     1501 gacgaccggt gccaattcct tgccgatgtc ggtcaggtga tactcgactt ttgggggaac
     1561 aacaggatag accgtcctgg cgatgatcag gtcgcgttcc agctcccgca gctgccggat
     1621 cagcatccgt tggttgatgt caggaaggat cttgttgaac tcgctcagcc gcagcgttcc
     1681 gttgtgcagc aaggaccaga tgatcgcaat tttccatctc ccccccacaa cagataacgc
     1741 taaatcttta gtgttgtaaa attctttctc ttggtattgg atcatcaggc agcctctttt
     1801 cataaaatta atcatagtga caattatgtc agtattcaca caacagtcat tatatagtat
     1861 tctttaacag aacgaaatac cattttttag aaagaaggaa attaaattat gaaaagcaga
     1921 gcagctgtcg cttttggacc aggcaaaccg ttggaaatcg tggaaataga tgtggcagag
     1981 ccaaaagcca aagaagtatt ggtgaagatt ctttacactt ccgtctgtca taccgatgcg
     2041 ttcactttgt cgggtgaaga tccggaaggt gttttccctg ccgtgttggg ccatgagggt
     2101 gccggtgtgg tcgttgccgt gggtgatgaa gtcacttccg tgaagcccgg ggaccatgtc
     2161 attcctcttt acacgccgga atgtggggaa tgcgaattct gtctgtccgg caaaacaaat
     2221 ctgtgttcag ccgtccgcga aacccaaggc aagggcttga tgccggatgg aacgacccgt
     2281 ttctcttaca acggagagcc gatttatcac tatatgggga caagcacttt cagtgaatac
     2341 accgttgtga atgaaatcaa cctagtcaac atcgataaag aggcgccatt ggataaagtt
     2401 gctttgttcg gttgcggcgt gacgaccggc ctcggggctg tcgaaaacac ggcaaaagtc
     2461 gaagaaggcg cagtgactgc agtcttcgga cttggagcga tcggactggc tgttatccaa
     2521 ggtttgaaaa aagccaaagc caagcgcatc atcgctattg atatgaatcc cgacaaatgg
     2581 gaactggcca aaaaaatggg cgccactgat ttcgtcaacc caagcgaaca tgatcgtccg
     2641 atccaggaag tcctgatcga aatgaccaac ggcggcgtgg attacagttt tgaatgcatc
     2701 ggaaatgtcg aagtcatgaa agccgctttg gaagcttgcc acaaaggctg gggcgaaagc
     2761 atcatcatcg gtgtcgcggg agccggcaaa gagatccata cccgtccgtt ccaattggtg
     2821 acaggacgcg tctggagagg ttctgcattc ggcggtgtca aaggccgcac ggaactgccc
     2881 ggaatggttg aggatttcat gaacggcgaa atcgacttgg acagcttcat cacgcatcac
     2941 ctgaatttca ccgacatcaa tgaagccttc gacctgttgc acaaaggcga gtcgatccgc
     3001 acgattttga cctatggaga ataaggacat gacaacaact ctgatcgaaa cgcataaagt
     3061 atttgacggc gaacaaagaa aataccgtca ctattctgaa acactggatt gcgatatggt
     3121 tttcagtcta ttcctgcccc aacaaacgga caataagccg acagatttaa tctggtggct
     3181 ttcaggcttg acgtgcaccg acgacaactt cagccaaaaa ggcgccttcc aaaaatacgc
     3241 aaaccagcac cacatggcgg tcgtcatgcc cgacacctcc ccacgcggaa cggctgtggc
     3301 ggatgatgaa ggttgggact taggccaagg cgccggtttc tatctgaatg ccacccagga
     3361 accgtgggca aaaaattatc ggatgtatgc ttatttgacg gaagaattgc ctcaacttgt
     3421 ctatgctctg attccgaatt tttcaggaaa agagagcatc atgggacact cgatgggtgg
     3481 ccacggcgcg ttggtcatcg gcttgaagaa tccggaacgc ttccgctcca tttcggcatt
     3541 tgcaccgatc ctgaatccgt cacaggttcc ctggggccaa aaagccttca ccgcctacct
     3601 ggggaccgat gaaacaaatt gggcggagtg ggacgcgacc agcttgatcg cggaaagccc
     3661 tgcagctccg cccatcctta tttcgcaagg gacagctgac aatttttatg atgtccaact
     3721 gaacgaggaa gccttcctgc aagccgctga aggcaagaaa gtgcgctacg aaaaactgga
     3781 cggttacgat cacagttact ttacgatcgc taccttcatc gaagaccatc tgcaattcca
     3841 taaagactgt ttgaagtaga aagcagcaac cataagcaca caaaaagccg ataaggtcac
     3901 ggatattccg tttccttatc ggctttttta ccgagctttt gtcaaacacc caggcaacta
     3961 ttttttcttc tggatccatt tcttagatgg cttagacggt ttagatgaag gcggtttgat
     4021 tagatttttc ttcagccagt tgaaatagta catatccggc acgaccgcaa agtcggtcat
     4081 tacattggaa atcgcgatgt agagttcttc ttcgctgtcc gcaagataat caacagcatc
     4141 gattgcttcc accatgctct ccagatcaaa ctcccgccgg ccaaaaactt ttttgcagct
     4201 tttgttttct ttctgtagat ctttgtaata gcgtttcgcg ctcaactcgt catcgtattt
     4261 caatgaaagc agaatcagcg gcaacagcat ctgagcggat gcttcaggga agcgctggta
     4321 aagctctttg gctttttcga tttgttccaa ctgacagtag atacccatca gaatgtagcg
     4381 gatccccata ttgtcagctt cgttcaaccg caaaatatct tcatactccc tcacggaagc
     4441 cgtcaacatc cggcgtttct gcaattcatc cgcatacagc gccttgatgc gcaagtatgt
     4501 cctggtttcg acgattcccc aatagtttcc gatggactcc tcatcatcca gaccgcgctt
     4561 ttgcatcact gcctttccga gtgtctccag tttcttgagt tcatgaatga attcgagtga
     4621 ttcaggatcc agtttaaata gccgggcttc caaattatcc ggatccaact tcaaggcacg
     4681 ctccaaaatc gctgcgtatt cgcgttcgga agccgcgaac tccagcttct ccagctcttc
     4741 agtcgctttt tcctcgtttg tcatttctcc gattacgaac gtttcgccct tggcactggc
     4801 ctcgttggaa agcttcgtga atgcttcaac tgcttcatcc acagagtcgt aatcctcgac
     4861 gtgattcaaa atgaatttgt tcaactgttt caatactttt tccgtttctt tcatgcaatt
     4921 catcctcccc aattgcagtt tgctaagtat attatacccg atttttgacg gcttgcgtta
     4981 aggagaagat accttccagc tgaaaagttt tactcagtac ggcactctgt tcgacgatcg
     5041 ccatgatgac gtcgacggat ttcgccatcg tttccagcgt aacgaattcg tatttgccgt
     5101 ggccgttttc ggttccggag aacaggttcg gggtcggcaa gcctttgtga gagatgatgc
     5161 agccgtccgt cccgccgcgc atcggcttga ataccggcgt gataccgcag gattcatacg
     5221 cctgcaccgc caagtcgacg acaatcggat gctgttcgat gatatcgcgc atgttgtagt
     5281 attgatcaaa caacgtcaag gaaatacgcg gttcgacaaa ctgggagttg aacgccgcca
     5341 cctgttcggt gaagaaacgc ttacgggcag cgaatcgctc acggtcatga tcgcggatgt
     5401 agtaggtctg tttgacttca tcgacggtcc cgctctggct agtcaggaag tagtagcctt
     5461 cgtagccgct ggtgcgttcc ggagtctcct cttgaggcaa agccgtcgca aattgactgg
     5521 cgaccagcaa agcgttgacc atcgtatcct ttgcggaacc gggatggacg ctcgtgccgc
     5581 ggatggtgag ttccgcttgg gccgcattga aattgtcgta ggtcagattt ccgatcggtc
     5641 cgccatcaat cgtgtaggcg aagtcggccg cgaaacggtc cacatcgaac agtttcgccc
     5701 cgcgagcccc aagttcttca tcgggtccga aagcaagacg gatcttcccg tgcttccgtt
     5761 ccggatgcgc gatgaattgc tcgcaggcgg tcacgatcga ggcaatgccg gctttgtcgt
     5821 cggcgcccaa cagggtcgtg ccgtccgtag tgatgaccgt ctccccgata tagttccgca
     5881 agcgcgggaa ttccttcggc gagaggataa ttttttcagc ctcgttcaag acgatatcgc
     5941 cgccatcata agcacaatgg acctgtggat ggatattgac tgagttgaaa tcggccgtgt
     6001 ccagatgcgc aatgaaaccg atgaccggca ctgcttctgc cgtattgctt ggcagggtcg
     6061 cggtcagaaa gccgttcgtt tcattatatt cgacctcctc cagaccgatt ctgatcagtt
     6121 cctttgccaa ctccaaggca aatgtcacct ggttatgcgt tgacggcacc gtgtagctgc
     6181 tgtcatccga acgcgtgtcg acctttgcgt atgcgatgaa tctttccaat aaattttcgt
     6241 acatatacct catccgcctt tctttgacgc ttttcatatc aatataagga aatatccgac
     6301 taacggtcgc cttctgaagg caagtccgtt aagcggatat tttctcaagt gcttgttttc
     6361 agtctattgt ccgtttactc ttccacgtaa gccgttcgga aatacatcgc tgcgcccact
     6421 ggttggatct gcacatcctt caaggacgga tcgaccaagt tggcgttggc gccttgatag
     6481 atcggcgcat tcacggcgtt ctcggcaatc aaggtttctt ccgccgccaa cagtgtttcc
     6541 caacgttgtt ccggatcgtt cgcatacgtg cttctggtgt cgtccaacaa ggcatcgtaa
     6601 ccttcatccg caaagcgcga actgttcagg ccgcttgtgg attcatacat ctccagaaag
     6661 gcaatcggat cggcgtaact cggggtatac gttccgtagg cgatgtcgta atcaccgttc
     6721 gtggtcgatt ccatccggct cttcaatggt acattgcgga tcgtgatcgt caaaccgggc
     6781 aagttctgtt gccattgtga ttgcagaaat tcggcagtcg tcttggaagt ctcggtatcg
     6841 gaagtcagca attcgatcga caatgtttcg attccgagct cttccttcgc ggtttcccac
     6901 tcggcctgtg ccgtttccag atcgtagact gccaaatcgc cgctctcttc gcggaaatct
     6961 tcatcggttt ccggattgaa ggcgtaattg gaaggcacaa aaccattcga aggaacggac
     7021 ccgtcattca aaacg
//
LOCUS       DAOMUJ010000032         7024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2842_length_7024_cov_2.672263, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000032 DAOMUJ010000000
VERSION     DAOMUJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_7024_cov_2.672263"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLT73_01605"
     CDS             <1..260
                     /locus_tag="PLT73_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM19108.1"
                     /translation="KVSRLIKRRSVAKEVADKGRILINGKQAKSSSTVKVGDEMTLQF
                     GNKTVVVRIDKIVETTKKDEAQEMYTLISETVAPRTEENRF"
     gene            368..802
                     /locus_tag="PLT73_01610"
     CDS             368..802
                     /locus_tag="PLT73_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRM19109.1"
                     /translation="MKEKTEKKTADNVTVMQNDFTKAQHSQKRIFQREKKAHMRRLSL
                     IFVVGAALIVPCLWKTVGNWLEIRKLDAAIVLAQTDKKQAEDENTQLTYEVKLLQDDE
                     YIAKLARGKYYLSRDGEIIFSLPEDNQTKMAEKQENAEDLDS"
     gene            890..1369
                     /locus_tag="PLT73_01615"
     CDS             890..1369
                     /locus_tag="PLT73_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HRM19110.1"
                     /translation="MSIEVGNKVSGKVTGITNFGAFIDLGGGKTGLVHISEISDTFVK
                     EIKDVLKVGDEVTVKVMSIADDGKIGLSIRKAVDKPAGEVSRPQRESRPYQKSTTPRP
                     GGSFSKGRKDFSAHQDKGLKNDFDSLMSSFLKDSEDRLTSIKRNTEGKRGGRGGRRG"
     gene            1458..2855
                     /gene="tilS"
                     /locus_tag="PLT73_01620"
     CDS             1458..2855
                     /gene="tilS"
                     /locus_tag="PLT73_01620"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRM19111.1"
                     /translation="MQEYILRLCKEVERHRLWEKESRLLVAVSGGVDSMALLGLIQQL
                     PREWQPQFAVVHVHHHLREESDDEFRMIKDYCRNQGIAFFAEHWSEEKHPKSNLEMAA
                     REFRYAFFADMMQKWPATHLVTAHHADDQLETILMRLVRGSTLKGISGIAFSRAFGRG
                     QLVRPLLSFQKDDLYRICGMAGIPYLEDSTNSELTFSRNRYRNSIIPLLQLENQQASR
                     HFGDFSEDLQDVLKVVQPIVADSFRSLFTRDDADWVLDLVGWQACDISLQRLVLSYFV
                     TEQLIPAGYDFRRSQLTAVMDLAKNQSPQKQISLVGGWQARKRYDKLIFLSPDSARTA
                     FSAFSEELELNKWVTLPYHGRIGLFHSDNAFSKELPKNALSVVIKDETAYMPITVRSR
                     RSGDKIVLNKDQPFTKKVSRLFVDKKIPDEERRKACIVTDGEGRILWVPGYAQSVWLS
                     ENDNEQTCYRLIYIK"
     gene            2874..3413
                     /gene="hpt"
                     /locus_tag="PLT73_01625"
     CDS             2874..3413
                     /gene="hpt"
                     /locus_tag="PLT73_01625"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRM19112.1"
                     /translation="MLHPDIEEVLYSEEEISAVVKDLGAQLTKEYEGKNPLVIGVLKG
                     AVMFMTDLSRAMDCDLELDFMDVSSYGAGMESSGDVKILKDLDTTVDGRDLLIVEDII
                     DTGRTLSYLIEIFKYRKAKSIKVVTLMDKKERRVVDLEADYIGINVPNEFVVGYGLDF
                     NEKYRNLPYIGILKTEVYE"
     gene            3536..5647
                     /gene="ftsH"
                     /locus_tag="PLT73_01630"
     CDS             3536..5647
                     /gene="ftsH"
                     /locus_tag="PLT73_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM19113.1"
                     /translation="MKKNNFLKSSGFYVLIFLAIIAVVSMITGGMETETSQEISSSEF
                     MDALNSEEVDNFTIQPGAGVYEISGEYRSEQELEVADSSSDLLLFDDTTSTTTKNFTT
                     RVLPNDETLKQITESAETTETQMVSLEEDQSGAWISILFSVLPIVIFIFFMYMMMGQA
                     GGGQGGNRNVMNFGKTKSEDATKKPIKVRFSDVAGAEEEKQELVEVVEFLKDPRRFTA
                     LGARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLF
                     DQAKKAAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFSGNEGIIVIA
                     ATNRSDVLDPALLRPGRFDRQILVGRPDVKGREAILKVHSRNKPLASNVDLKVVAQQT
                     PGFSGADLENLLNEAALVAARRNKTKIDALDVDEAHDRVIAGPAKRDRVISKREREMV
                     AYHEAGHTIVGMVLSDARIVHKVTIVPRGRAGGYAIMLPKEDRFLMTKDELLEQVVGL
                     LGGRVAEEVVFNVQTTGASNDFEQATGLIRSMVTEYGMSDKLGTVQYEGNHQVFVGRD
                     YGQTKAYSEDIAYQIDSEVRRMMNDAHIRARQIIEEHREQLNLIAEKLLELETLDEKT
                     IRSLFETGQMPSDSNTEEYPSEKAQSFEEAKRALEKKEAEKIHQEEQMLADDESQDSL
                     PAADGKAENESENQDTDTEEK"
     gene            5808..6710
                     /gene="hslO"
                     /locus_tag="PLT73_01635"
     CDS             5808..6710
                     /gene="hslO"
                     /locus_tag="PLT73_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM19114.1"
                     /translation="MSSYLIKALAYEGQFRAYAIDATEAVAEAQKRHDTWSASSAALG
                     RTMVGTLLLAAAGLKNEEKMTVIVNGDGLGGRILADANGKGDIKAYISNPNVNLALNG
                     DGKLDVRAVVGTEGTLTVIKDLGMKEPFTGQVSLVSGELGEDFTYYMANSEQIPSAIG
                     LSVLVDTDDSIKAAGGFMIQVMPDASDEAITKVEQNIAAIPLVSRLMENGETPEQILA
                     RILGEENVEVLEKMPVRFHCDCSRERFAGGLSSIGKDELHEMIVEDHGAEVVCHFCGE
                     KYHYSEADLQELVTAIDEKRAANI"
     gene            6820..>7024
                     /locus_tag="PLT73_01640"
     CDS             6820..>7024
                     /locus_tag="PLT73_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM19115.1"
                     /translation="MVKIVSSIASLIGETPIIKLNKVVPEGVADVYVKLESFNAGGSV
                     KDRIALNMIEVAEEEGLLKPGYTI"
BASE COUNT         2102 a         1365 c         1845 g         1712 t
ORIGIN      
        1 tgaaagtatc ccgcttgatc aaacgcagat cagtagctaa agaagtggcg gacaaagggc
       61 gcatcctgat caacggcaag caagcaaaat cgagttcgac tgtcaaagtc ggagatgaaa
      121 tgacgctgca gttcggaaac aaaaccgtcg tcgttcgcat cgacaaaatt gtggaaacga
      181 ctaaaaagga tgaagcgcag gaaatgtata ccctcatcag cgaaacggtc gcacccagaa
      241 cggaagaaaa tcgcttctga tgatgcgaaa ggccatggac aatccgcgcc gtttcttcta
      301 tacttttaca tgagtaaaac tattcagaga taacgggatt gtggaagttt tcaaggaggc
      361 gatcagggtg aaggaaaaga cggaaaaaaa gacagctgac aacgtaaccg tgatgcaaaa
      421 tgattttacg aaggcgcagc acagtcagaa acgtatattt cagagggaaa agaaagccca
      481 tatgagaaga ttatccctga tttttgtcgt cggcgctgcc ctgattgtgc cgtgcctgtg
      541 gaaaacagtc ggaaactggc tggaaatccg taagttggat gctgcaatcg ttttggccca
      601 aactgataaa aaacaggctg aggacgaaaa tacgcaattg acttatgaag tcaaactgtt
      661 gcaggatgac gaatacatcg ccaaattggc acgcggtaaa tattatttga gcagagacgg
      721 cgagatcatc tttagtttgc cagaggataa tcagacaaaa atggccgaga aacaagagaa
      781 tgcggaagat ttggattcat gaatacagcg atggataaag attctatttt tttctgcata
      841 atccatggta taattagttg agcaaaaatg aaggaggaac ttttttttta tgtcaataga
      901 agttgggaac aaagtatcag gaaaagttac agggattaca aattttggtg cattcattga
      961 tttaggtggc ggtaagacag gactagttca tattagtgaa atttctgata ctttcgtaaa
     1021 ggaaataaag gacgttttga aggttggcga tgaagtgacc gttaaggtga tgtccattgc
     1081 ggatgatggg aagatcggtt tatccatccg taaagctgtg gataagccag caggggaagt
     1141 ttccagacct caacgagaaa gcagaccgta tcaaaaaagt acaactcctc gcccgggagg
     1201 ctctttttct aaaggcagaa aagatttctc agcccatcag gataaaggtc ttaaaaacga
     1261 ctttgactcg ttgatgagca gcttcttgaa ggatagcgaa gaccgtttga cttccatcaa
     1321 acgcaatacg gaaggcaaac gcggaggccg tggcggcaga cgtggataga aaacagtaat
     1381 ttctagagga tgggcaaaag ggaaaccgat tgtcacaccc tcttttttgc tttacgaaga
     1441 aagaaagcag gggtgctatg caggaatata ttctgcgtct atgcaaagaa gtggaacgtc
     1501 accgactatg ggaaaaagaa agccgcttgc ttgtagcggt atccggcggc gtagattcca
     1561 tggcattatt ggggctaatc caacaattgc cgcgggaatg gcaaccacag ttcgcagtgg
     1621 tgcatgtcca ccatcatctc agggaagaat cagatgatga attccggatg attaaagact
     1681 actgcaggaa tcaaggtata gcttttttcg ccgaacattg gtcagaagaa aaacatccga
     1741 agtcaaacct ggaaatggca gcaagggaat tccgctatgc cttctttgcc gacatgatgc
     1801 aaaaatggcc agcgacccac ttggtgacag cgcatcatgc ggatgatcag ctggagacga
     1861 ttctgatgcg tttggtgagg ggaagcacgt tgaagggcat atcaggcatc gctttttcac
     1921 gcgcttttgg ccgagggcaa ctcgttcgtc cgttgctgtc gttccaaaaa gacgatctct
     1981 accggatctg cgggatggcc ggaatccctt atctggaaga cagcaccaat tcggaactga
     2041 cgttttcgcg gaacagatac cggaacagca tcatcccgtt gctgcagttg gaaaaccaac
     2101 aggcatccag gcatttcggc gatttttcgg aagatctcca ggatgtgttg aaggtcgttc
     2161 agccaattgt agcggattct tttcgctcgt tgttcaccag agatgatgcg gattgggttt
     2221 tggatctggt cgggtggcag gcctgtgaca tttccttgca gcggcttgtg ctcagctatt
     2281 ttgtgacgga acagctgata ccggcaggat atgacttccg gaggagccaa ctgactgctg
     2341 tgatggatct tgccaagaac caatctccgc aaaagcaaat atctttggtg ggagggtggc
     2401 aggccagaaa aaggtatgat aaacttattt tcttatcccc tgattcggcc agaaccgctt
     2461 tttctgcttt ttctgaagaa ttggagctaa ataaatgggt aaccttgcca tatcatggta
     2521 gaataggatt atttcatagc gataatgcgt tttcaaaaga acttccgaag aatgctctaa
     2581 gcgtcgtcat caaggacgaa acggcatata tgcctattac tgtgcgttcc cggcgttcgg
     2641 gggataagat cgttttgaac aaagaccagc cattcacaaa aaaagtgagc cggctttttg
     2701 tggataaaaa aattccggat gaagaaagac gaaaagcctg tatcgtcact gatggcgagg
     2761 gacgtatctt atgggtgccg ggctatgccc agtccgtctg gttgtccgag aatgacaacg
     2821 aacaaacgtg ttacagattg atctacataa aataattttt tgaggtgaaa gaaatgttgc
     2881 atcctgatat cgaggaagta ttgtacagtg aagaagaaat cagcgcagta gtcaaagact
     2941 tgggcgcgca attgacgaaa gaatatgaag ggaaaaatcc attagtgata ggtgtactga
     3001 aaggagccgt catgttcatg accgacttat cgcgtgctat ggactgcgac ctggagctgg
     3061 atttcatgga cgtttccagc tatggagcgg gaatggaatc aagcggcgat gtgaaaatcc
     3121 tgaaagattt ggatacgacc gtggacggac gcgacctgtt gattgtagag gacatcatcg
     3181 acactgggcg gactttaagc tacttgattg aaatattcaa ataccgcaaa gccaaatcca
     3241 taaaagtagt cacgttgatg gataaaaaag aacgccgcgt agtggatttg gaagcggatt
     3301 acattggtat caatgttccg aatgagttcg ttgttggata cggattggac ttcaacgaaa
     3361 agtaccgcaa tctcccttat atcggtattc taaaaaccga agtttatgag taaaatcatt
     3421 cgttaaaatt ggtcagatat gatatgatta atgggatact agtatattga gcacacaaaa
     3481 aagatgagac gtaagataaa gtctgcgaag aacgatatct gacgaggagg ttctaatgaa
     3541 aaaaaataat tttcttaaaa gcagcggctt ttatgttctg atcttccttg cgatcattgc
     3601 agtggtttcg atgattacag gcggcatgga aacagagacg agccaagaaa tttcatcaag
     3661 cgaatttatg gatgcattga actcggaaga ggtggataat tttacgatcc aaccgggtgc
     3721 aggcgtttat gaaatcagcg gtgagtaccg cagcgaacaa gaactggaag tggctgattc
     3781 gtcaagcgat ttactattgt ttgatgatac gaccagtaca acaacaaaga attttacaac
     3841 cagagtttta ccgaatgacg aaacactgaa acaaatcacg gaaagcgctg aaacgacgga
     3901 aacacaaatg gtgtcgttgg aagaagatca gtccggtgca tggatcagta tcctgttcag
     3961 cgtattaccg atcgtcatct tcatattctt catgtacatg atgatgggtc aagctggagg
     4021 cggacaaggc ggtaaccgca atgtgatgaa cttcggcaaa acgaagtctg aagatgccac
     4081 caaaaagcct atcaaagtac gtttttctga cgttgctggt gcagaggaag agaaacaaga
     4141 gcttgttgaa gtagttgaat ttttgaagga tccacgtcgt ttcactgcac ttggcgcaag
     4201 aattccggca ggcgttttgc ttgaaggacc tccaggtaca ggtaagacct tattggccaa
     4261 agctgtcgct ggtgaggctg gtgtgccttt cttctcgatt tccggttctg aattcgtaga
     4321 aatgtttgtc ggtgtcggtg ccagccgtgt gcgtgacttg ttcgatcaag ccaaaaaagc
     4381 tgctcctgca atcatcttca tcgatgaaat cgatgcagtt gggcgtcaac gtggcgccgg
     4441 tatgggtggc ggccatgatg aacgcgaaca aacactgaat caactgttgg ttgagatgga
     4501 cggtttctca ggtaatgaag gcatcatcgt aattgccgca acaaaccgtt ccgatgtact
     4561 cgatccggca ttgcttcgtc caggccgttt tgaccgccag attttggtgg gaagaccgga
     4621 tgtaaaaggc cgtgaagcga tattgaaagt acattcccgc aacaaaccgt tggcaagcaa
     4681 tgtcgatctg aaagttgtcg cgcaacaaac accaggcttc tctggagcag atctggaaaa
     4741 cctgttgaac gaagctgcgt tggttgcagc cagacggaac aaaacaaaaa ttgatgccct
     4801 tgatgtggat gaggcgcatg accgtgtcat cgccggccct gccaagcggg atcgtgtcat
     4861 cagcaagcgc gaacgcgaaa tggttgccta ccatgaagcc ggccacacga ttgtcggtat
     4921 ggtactgagc gatgcgcgca ttgtccacaa agttacgatc gttccacgcg gtcgcgcggg
     4981 tggatatgca atcatgcttc cgaaagaaga tcgattcctc atgacgaagg atgagttgtt
     5041 ggaacaagtt gtcggattgc ttggtggacg tgtagcggag gaagttgtct tcaacgtcca
     5101 aactacaggc gccagcaacg acttcgaaca ggctacgggc ttgatcagaa gcatggttac
     5161 cgaatacggt atgagtgata agttgggtac tgtacagtat gaaggaaatc atcaagtatt
     5221 cgtcggtcgt gactacggtc aaaccaaagc atactctgaa gatattgctt accaaatcga
     5281 ctcggaagtc cgtcgtatga tgaatgatgc gcatatcaga gcgcgtcaaa tcatcgaaga
     5341 acaccgtgaa caactgaatt tgatcgctga gaaattgttg gaactggaaa cattggatga
     5401 gaaaacaatc cgcagcctgt tcgaaaccgg ccaaatgcct tcggactcaa atacagaaga
     5461 atatccaagc gaaaaagcac agtcttttga agaggccaaa cgggcccttg aaaagaaaga
     5521 agctgaaaaa attcatcagg aagagcagat gcttgctgat gatgaatccc aagattcatt
     5581 acctgctgcc gatggaaaag cagaaaatga atcggaaaat caagacacag atactgaaga
     5641 aaaataaaac aaatagaatt ttgcgaaaag tctgggacga aaatcccgga ctttttgtca
     5701 tcaccgcgtg gtttcacagt accaagtgag caaagtgtgg ttgctttcag cttgacctat
     5761 agtagaatga tagagaaaca atttaagaat gcaatggagg attatacatg agtagttatt
     5821 taattaaggc gttagcctat gaaggacaat tccgggccta tgcgatcgac gctacagaag
     5881 cggttgctga ggctcaaaaa aggcatgata catggagtgc ctcttctgca gctttgggcc
     5941 gcacaatggt ggggactttg ttgctggctg cggctggatt gaagaatgag gaaaaaatga
     6001 ccgtgatcgt gaacggagat ggcttggggg gccgtatact cgctgatgcg aacggaaaag
     6061 gcgatatcaa agcctatatt tctaacccga atgtcaacct tgcattgaac ggtgatggca
     6121 aactggatgt acgcgcagtt gtggggactg aaggtacttt gaccgtcatc aaagatctgg
     6181 gtatgaagga accgttcacg ggacaagttt ctctggtcag cggcgaattg ggcgaggatt
     6241 tcacctacta tatggcaaat tctgagcaaa tcccttcagc tatcgggtta agcgtactgg
     6301 ttgatacgga tgattcgatc aaagcggctg gtggattcat gattcaagtg atgcctgatg
     6361 ccagcgatga agccatcaca aaagtggaac aaaatatcgc agctattccc ttagtctcca
     6421 gattgatgga aaacggtgaa acgcctgaac agatcttggc gcgtatattg ggcgaagaaa
     6481 atgtggaagt cctggaaaag atgccggttc gtttccactg cgattgttca agagaacgtt
     6541 tcgctggtgg tttgagttca atcggtaaag atgaacttca tgagatgatt gttgaggatc
     6601 acggggctga agtggtctgc catttctgtg gcgaaaaata ccattattcg gaagctgatc
     6661 ttcaggaatt ggttacggcc attgatgaga agcgtgccgc caacatctaa attagccagc
     6721 gtttcacttg taatgcggtt tcgcgtaacc tacaatgaaa ctaatctgtt cgtttgatgg
     6781 aagtgatcag taaaatttag cttagctgga ggaatcgaca tggtaaaaat agtctcttca
     6841 attgcgagtt tgatcgggga aacaccgata atcaaactga ataaagttgt gcctgagggt
     6901 gtggctgatg tttacgtcaa gttggagtct ttcaacgcgg gcggaagcgt gaaagatcgc
     6961 atcgctctga atatgattga agtggctgaa gaagaaggac ttctgaaacc cggttataca
     7021 atcg
//
LOCUS       DAOMUJ010000033         7020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2844_length_7020_cov_2.500359, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000033 DAOMUJ010000000
VERSION     DAOMUJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2844_length_7020_cov_2.500359"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..503)
                     /locus_tag="PLT73_01645"
     CDS             complement(225..503)
                     /locus_tag="PLT73_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM19116.1"
                     /translation="MENKLQKYFDTQMDTPEFAEAWRETEASYQAANILLKIRAEKQL
                     TQSELANLTGKKQSYIARVEKGTQNISVQTLNDIVESAGGKLKIEVVV"
     gene            complement(508..858)
                     /locus_tag="PLT73_01650"
     CDS             complement(508..858)
                     /locus_tag="PLT73_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM19117.1"
                     /translation="MKKYNFDVYEKENGEAPFLVYLDSLDVKSRAKVLRAITIVEDFG
                     AQSPPGYIDHLGDGIYELRVKFSSNIFRCLYFHFHNNKYIITHGFTKKTQKTPSREIT
                     KSKEYRKDYLERNE"
     gene            complement(1056..1916)
                     /locus_tag="PLT73_01655"
     CDS             complement(1056..1916)
                     /locus_tag="PLT73_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM19118.1"
                     /translation="MAKVKKTTGYKAQRRSSLATVYAVLAVLSVIWLFPIVWIVLTSF
                     RAEGGAFVPYIIPKSFTLDNYRILLQNTTGNYPFVRWFLNTLFVSVISCILSTFITIA
                     MSRLRFKMRKPFLKVALVLNMFPGFMSMIAVYYILKALNLTESLLALILVYSAGSALG
                     FYIAKGFFDTIPMALDESAMIDGANKWEIFTKITLPLSKPIIVYTSLLAFMGPWMDFI
                     FAKIIMGDNIPNYTIAIGLFTMMTRTTANSLFMAFTAGCVLIAIPITILFIFMQRFYV
                     EGVTSGSVKG"
     gene            complement(1921..3270)
                     /locus_tag="PLT73_01660"
     CDS             complement(1921..3270)
                     /locus_tag="PLT73_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM19119.1"
                     /translation="MFKKKSYGQQMTFRELFKEGDVATKLSFVVMGAANLANKQHLKG
                     LIFLFSQIAFFYWLIRNGLRALSMLATLGTQSQGLVFDERLGIEVLQEGDNSMLLLLF
                     GIAAIVICLLLVGLYVINLKSARNIHELKKAGKKIPSTMDDLASLLNERFHATLMTIP
                     LLGVLLFTVLPLLYMISIAFTNYDHTHLPPKNLFTWVGFVNFGNVISGNMADTFFPVL
                     GWTLIWATLATVTCFFFGILLALLINTKGLKFKGVWRTIFVTTMAVPQFISLLVMRNL
                     LNGAGPINATLLNLGLIDSAIPFLTDPIWAKITVIVVNMWIGIPATMLVSTGIIQNLP
                     TDQIEAARIDGANKLQIFRNITFPQILFVMMPALIQQFIGNINNFNVIFLLTGGGPSN
                     SDFYGAGSTDLLVTWLYNLTVNTMDYNLASVIGILIFILSAVFSLLAYTRTNSFKEG"
     gene            complement(3350..4624)
                     /locus_tag="PLT73_01665"
     CDS             complement(3350..4624)
                     /locus_tag="PLT73_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM19120.1"
                     /translation="MKTNWKKYFGSGLVLGASALILGACGGTADTSDTGSAENTSGEV
                     VVEGDVKLWVDTDHVEVFKGIVAEFEKEFPEVTVEVAAGSSADAKKDVSKDPKAAADV
                     FMMPHDQVGQMAEAGLLYPNTKYADEVKANNVESAVEGVTWNDELYGYPYGVESQVLY
                     YNKAKLTEDDVKSWTTLTEKGKIGTNFAEAGANYIFGPLFMSNGLYLYGETGEDPSGT
                     NFNDEKGAEVLAWIAAQKNNPGVIQSGAEALANLEAGVSDAFLSGPWSKNDVTKALGE
                     NMGVAAYPTVDFGSGEVQMKAFLGVKLFAVNQQTDAPLASMALANYLSNETSQLIEFQ
                     EMGVIPSNKVVQANAEVQEDVVAKAVMEMSQSTHSVVMPKVPEIVSFWPAMDAIINDA
                     YKGNITADEYMEKLDKLVEDTSKVTEPEEEKE"
     gene            4923..6686
                     /locus_tag="PLT73_01670"
     CDS             4923..6686
                     /locus_tag="PLT73_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002398236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRM19121.1"
                     /translation="MNTSALYHRPESEFAYLYDPETMHVRLRTARGDVREMGLLYGDP
                     YFLDKEEWFRKPLQMIKTLSTDLYDYWFVEVTAPLKRLSYAFALKGHDGISVFYGDHG
                     VYPLEEDYLRMPNNYFRMPFFHEVDRYKTPEWVKKTVWYQIFPERFANGDPSNDPEGT
                     LAWGSADPSRENFFGGDLQGVIDHLDYLEDLGINGIYFCPIFEASSNHKYDTIDYLKI
                     DPAFGDAETFKKLVEECHKRGMKVMLDAVFNHIGDSSPQWQDVLAHGEDSQYVDWFHI
                     NEFPASYKRGENFEDAYDITYDVFAANPHMPKLNTANPEVQDYLLNIARYWIEAFDID
                     AWRLDVANEVDHAFWKKFRIVCDEAKKDFYILGEIWHSSQNWLQGDEFHAVMNYAFTD
                     AIMGYFVKNELSLSKMVSEMNNQLMLYRNQTNQMQFNILDSHDTPRLLTEANEDKDVM
                     KQAMAFTYIQPGVPCLYYGDEIGLTGGMDPDCRKCMVWEEEKQDRDLHRFVKELNGIR
                     KEQQKILSEGTVYWRQVSEESGVVIFERMLGGEVIRGAFNTGEHTARVELIGEALFSN
                     LADVSDGAVELARKGFVLMKE"
     gene            6727..>7020
                     /locus_tag="PLT73_01675"
     CDS             6727..>7020
                     /locus_tag="PLT73_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010713451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM19122.1"
                     /translation="MKKHWWQEVVVYQIYPRSFRDSNGDGIGDIAGMTEKLDYLEKLG
                     IGAIWLSPVYQSPNDDNGYDIADYEAILDEFGTMEEMEHFIAEADKRNIKVVMD"
BASE COUNT         1844 a         1669 c         1596 g         1911 t
ORIGIN      
        1 ttctctgctc accatcacta acacctcacc aatgatatag gtttttgtca ctgctttttt
       61 atgtacctct agtttaccat acacaaactt tacagggcac acctgagtgg aggcgattac
      121 caaaaatgtt gaacaacaca agccaaaaag gcgtagttgt ctgtaaacga gacagcccca
      181 cctttttgtg ttttcgcatg tgcggttgca gaattcacac aattttaaac gactacttct
      241 atttttaact ttccgcctgc agattccaca atatcattca atgtttgtac actaatattt
      301 tgagtacctt tttcaactcg ggcaatgtaa gattgttttt tcccagttag attagctaat
      361 tcagattggg ttaactgttt ttctgctcta attttcaaaa gaatgtttgc cgcttgatag
      421 ctggcttcgg tttctctcca tgcttcagca aattctggcg tgtccatttg ggtatcaaag
      481 tatttttgta atttattttc catcttttta ctcattcctt tccaggtagt ctttacgata
      541 ctctttagat ttagtgattt ctcttgacgg ggttttttgt gtttttttcg tgaaaccatg
      601 ggtgataata tacttattat tgtgaaaatg aaaatataag catctaaata tattgcttga
      661 gaatttcact cgaagttcgt aaatgccatc gcctaagtga tcgatatacc ctggtggaga
      721 ctgcgctcca aaatcttcca caatagttat tgcgcgtaat actttagctc tggattttac
      781 atccaaacta tctaaataca ccaaaaaggg agcttcaccg ttttcttttt catagacatc
      841 aaaattgtat tttttcatac cctcattata acttataagt gattaaaagt aaatatcccc
      901 tcaacttcag caccttgaaa atgaactcag actctaggca acccctgtcc tattttcatt
      961 tatccagatt catcgggcag aaaacctcgg ccttacagaa aatcccaaaa ggcgctccgg
     1021 caagagcgcc ttttgggatt tttcactata tattcctatc ctttgactga tccggaagtc
     1081 acaccttcca catagaagcg ttgcatgaag atgaacagaa tcgtgatcgg gatggcaatc
     1141 agcacacagc ctgcggtgaa ggccatgaac agcgagttcg cggttgtgcg ggtcatcatc
     1201 gtgaacagcc caatcgcgat cgtgtagttc gggatgttgt cgcccatgat gatttttgcg
     1261 aagataaaat ccatccatgg tcccatgaaa gccagcaagg acgtgtacac gatgatcggt
     1321 ttggacaacg gcagtgtgat ttttgtgaag atctcccact tgttggcacc gtcgatcatg
     1381 gccgattcat ccaatgccat cgggatcgtg tcgaagaatc ctttggcgat gtagaagccc
     1441 agcgccgagc cggcggaata gaccagaatc aaagcaagca ggctttccgt caggttcagg
     1501 gctttcagga tgtagtacac ggcgatcatg ctcatgaatc cggggaacat gttcaggacc
     1561 aacgcgactt tcaggaacgg tttccgcatc ttgaagcgca ggcgcgacat tgcgattgtg
     1621 atgaaagtcg acagaatgca actgattacg gaaacaaaca acgtattcag gaaccagcgc
     1681 acaaacggat aatttccggt tgtgttctgc aaaagaattc tgtagttatc caacgtgaat
     1741 gattttggaa taatgtacgg aacgaacgcg ccgccttctg cccggaagct ggtcaaaacg
     1801 atccacacga tagggaatag ccaaatgacc gacagaacag ccaggacggc atagactgta
     1861 gccagcgaag accgccgttg cgctttgtat cctgttgttt tctttacttt tgccattttt
     1921 ttagccctcc ttgaatgaat tcgttcttgt gtaagccagc agactgaaca ctgcggacag
     1981 gatgaagatc aggataccga tgacggatgc caaattgtag tccatcgtat tgaccgtcaa
     2041 gttgtacagc cacgtaacca acaagtccgt cgaacccgcg ccgtaaaagt cggagttcga
     2101 aggtccgcct ccggtcaaca ggaaaatgac gttaaagttg ttgatgttgc cgatgaactg
     2161 ttggatcaac gcaggcatca tcacgaacag aatttgcggg aaagtgatgt tcctgaaaat
     2221 ctgcagtttg ttggcgccat cgatccgggc cgcttcgatc tgatccgtcg gcaggttttg
     2281 gatgatcccc gtcgaaacga gcatcgttgc cggaatcccg atccacatgt tgacgacaat
     2341 gacggtaatt tttgcccaaa tcggatccgt caaaaatgga atggctgaat cgatcaggcc
     2401 caagttcaac aatgtcgcgt tgatgggacc cgcgccattc aagagattac gcatcaccaa
     2461 cagtgaaatg aactgcggaa cggccatcgt ggtgacgaaa attgtgcgcc aaacgccttt
     2521 gaatttcagg cctttggtat tgatcaaaag tgccagcagg atgccgaaga aaaagcaagt
     2581 gactgttgcc agcgtcgccc aaatcagcgt ccagcccaag accgggaaga aggtgtctgc
     2641 catatttccg gaaatgacgt taccgaagtt tacaaagccg acccaggtaa aaaggttttt
     2701 cggcggcagg tgcgtgtggt catagttcgt gaaggcgatc gaaatcatgt agagcagcgg
     2761 caggaccgtg aacaaaagta cgcccaacaa cggaatcgtc atcaaggtgg catggaatcg
     2821 ttcattcaac aaactcgcca aatcatccat cgtgctcggg attttttttc ctgctttctt
     2881 cagttcatgg atattccttg cgcttttcag attgatgacg tacaaaccaa caaggagcaa
     2941 acaaatcaca atggcagcaa tcccgaataa tagcagtaac atcgagttgt cgccctcttg
     3001 cagcacttca atccctaggc gttcgtcaaa aacgagccct tgcgattggg tacccaacgt
     3061 cgccaacatg gatagcgccc gcagtccgtt gcggatcagc caatagaaga aggcgatctg
     3121 cgaaaacagg aaaatcaaac cttttaggtg ttgtttattg gcaagattgg cagctcccat
     3181 gacaacaaag gaaagttttg ttgcgacgtc cccttctttg aacaattccc gaaaggtcat
     3241 ctgctggccg taactttttt tcttgaacat ggcttcccca ctcactttca tcattttaaa
     3301 taagagggac gaaagatgcg tgctccttcg ccctcccttt taatttttgt tattcttttt
     3361 cttcttcagg ttctgttact ttggatgtgt cttctaccaa tttgtccagt ttttccatgt
     3421 attcgtctgc ggtgatgttg cctttgtatg catcattgat gatggcatcc atcgctggcc
     3481 agaacgaaac gatttccgga actttaggca tgacgactga atgagtggat tgggacattt
     3541 ccatgactgc tttcgccacg acatcttctt gcacttccgc atttgcctga acgactttgt
     3601 tcgatgggat aacgcccatt tcctggaatt caatcaattg ggaagtttca ttggacaggt
     3661 agttcgccaa agccatggat gctaaaggtg catctgtctg ttggtttacc gcaaataatt
     3721 tgacgcccaa gaaagctttc atctgaactt cgccgctgcc gaaatcgact gtcgggtaag
     3781 ccgcaacacc catattttca cccaatgctt tggtgacatc atttttcgac caaggtccgg
     3841 aaaggaatgc gtccgaaacg cctgcttcca agttggctaa ggcttcagca ccggactgga
     3901 tcacgcctgg attgttcttt tgagctgcga tccatgcaag gacttcagca cctttttcgt
     3961 cattaaagtt ggttccgctt ggatcttcgc cggtttcacc gtacaagtaa agaccgttac
     4021 tcatgaacag cggtccgaag atatagttgg ctcctgcttc agcaaagttt gtgccgattt
     4081 tgcctttttc agtcaaggtc gtccagctct tcacatcatc ttcagtcagt ttcgctttgt
     4141 tatagtagag aacttgcgat tcaacgccgt atgggtagcc gtaaagctca tcgttccacg
     4201 ttacgccttc gacagccgat tccacgttat ttgcttttac ttcatctgca tattttgtgt
     4261 tcggatataa cagacctgct tcagccattt gaccgacttg gtcgtgcggc atcatgaaaa
     4321 cgtcagccgc tgcttttgga tctttcgaga catctttctt tgcatcagct gagcttcctg
     4381 ccgccacttc aaccgtaact tcagggaatt ctttttcgaa ctctgctacg atccctttga
     4441 agacttccac gtgatctgtg tccacccaca atttgacgtc gccttccaca acgacttctc
     4501 cggatgtgtt ttcagctgag cctgtatcgc ttgtatcagc tgtgccgccg catgcgccca
     4561 agattaaagc agatgccccc aacactaaac cgcttccaaa atactttttc caattcgttt
     4621 tcacttactt gtcccccttg aaatgaattt cctttacatg tttgattatg caaccgattg
     4681 cttcaatagt caagctttat ttcttaaaaa aagaaaatgg ttcattttcc gacagtgttt
     4741 gattcgtccc aaaaagctta cttatatagt tatataaagc ttttccggtg aacaataacc
     4801 catcggacgc cattccctaa ctaaaaaaac aaaatgcaac cgattgctaa aagttcttgc
     4861 atcctactat gcgatgtgac acaatgtggg aaaacaaaga atgaaaagga gccgatacta
     4921 ctatgaacac ttccgcactt taccatcgcc ctgaaagcga atttgcctac ctatatgacc
     4981 cagaaacgat gcacgtccgt ctgcgtactg ctcgtggaga cgtccgcgag atgggtttgc
     5041 tttacgggga tccttatttt ttggacaaag aagagtggtt ccgcaaaccg cttcagatga
     5101 tcaaaaccct ttccactgat ctttacgatt actggttcgt cgaagtcacg gcccccttaa
     5161 agcgcctgtc ttatgcattc gccttgaagg gccatgatgg gatttccgta ttttacggcg
     5221 atcacggcgt ttatccatta gaagaagact atttgcggat gccgaacaac tactttcgga
     5281 tgcccttctt ccatgaagtc gatcgctaca aaacaccgga atgggtcaaa aaaacagttt
     5341 ggtatcagat tttcccggaa cgttttgcca atggggatcc gagcaacgat ccggaaggca
     5401 cgctggcttg gggttcggca gatccaagcc gcgagaactt cttcggcggc gacctgcagg
     5461 gggtcatcga tcaccttgat tatttggagg acttgggtat caacggcatc tatttctgcc
     5521 cgatttttga agcttcttcc aaccataagt acgatacgat cgattacctg aagatcgacc
     5581 cggctttcgg cgatgcagaa actttcaaaa aactcgtgga agaatgccac aagcgcggca
     5641 tgaaagtcat gctggatgcc gtcttcaatc atatcggtga ttcttcgccg caatggcagg
     5701 acgtacttgc gcatggggaa gattcgcaat acgtcgactg gttccacatc aatgagtttc
     5761 cggcttccta caaacgcggt gaaaatttcg aagatgccta cgacatcact tacgatgtct
     5821 ttgccgccaa cccgcatatg ccgaagctga acacggccaa tccggaagtg caggattatc
     5881 tgctgaacat cgcccgttac tggatcgaag ctttcgatat cgacgcttgg cgtttggatg
     5941 tcgcgaacga agtcgatcac gctttttgga aaaaattccg gattgtctgc gatgaagcca
     6001 aaaaagattt ctacattctc ggcgaaattt ggcattcttc ccaaaactgg ttgcagggcg
     6061 acgaattcca tgccgtgatg aactacgcct tcactgatgc gattatgggt tatttcgtga
     6121 agaatgaatt atcattgagt aaaatggttt cggaaatgaa caaccagctg atgctctacc
     6181 gcaatcagac aaaccagatg caattcaata tcctcgattc ccacgataca ccgcgtctat
     6241 tgacggaagc caacgaggac aaagacgtca tgaaacaagc gatggcattt acttatatcc
     6301 agcctggcgt tccatgcctg tactacgggg atgaaatcgg tttgaccggc gggatggacc
     6361 cggattgccg aaagtgcatg gtctgggaag aagaaaaaca ggatcgcgat ctgcaccgtt
     6421 tcgtgaaaga actgaacggg atccgcaaag agcaacaaaa aatcctttcg gaaggcactg
     6481 tttattggcg ccaggtatcc gaagaatccg gcgtagtcat ctttgagcgc atgctgggcg
     6541 gcgaggtcat ccgcggcgcc ttcaataccg gcgagcacac ggcacgggtc gagctgatcg
     6601 gagaggcttt gttctcgaac cttgcggacg tatcggacgg ggctgtcgaa ttggcgcgca
     6661 aaggttttgt gttgatgaaa gaatagtcac tgcctattta gaataaataa gagatgaggt
     6721 tgcagcatga aaaaacattg gtggcaagag gttgtcgtgt atcagatcta tccgcggagc
     6781 ttcagggaca gcaacggcga cggcatcggt gatatcgccg gcatgaccga aaaattggac
     6841 tatctggaaa aattgggtat cggagcgatt tggctttcgc ctgtctacca gtcgccgaac
     6901 gacgacaacg ggtatgatat tgccgactac gaagccatcc ttgacgagtt cggcacgatg
     6961 gaagagatgg agcattttat cgcggaagcc gacaagcgca acatcaaagt tgtaatggat
//
LOCUS       DAOMUJ010000034         6997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2856_length_6997_cov_2.114088, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000034 DAOMUJ010000000
VERSION     DAOMUJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_6997_cov_2.114088"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PLT73_01680"
     CDS             <1..1526
                     /locus_tag="PLT73_01680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM19123.1"
                     /translation="TYLEENKNKIAGIFLTHGHADAVGALPYLLQNIDAPVFGTGLTI
                     ALAKLAVDSTGLATGFEDYHVIDENTEIEFENTSVRFFRTTHTIPDSVGICVKTEEGS
                     VVYTGDFKFDQSASPMYRTDYGKITDIGEGKVLALLSDSGDAESTTENVSDRKIEEEM
                     LDTFINAEGRIIVSCVASNILRIQQVFDVALQANRKIFLTGPTLVETVDVAMKLGKLV
                     LPTPDLLVNVKNINDYRDGQVLILETGNSGEPLEALQRMSKGRHKQVNLKEGDLVYIT
                     TTPSTAMETTVAKTKNLIYRAGASVREISGTYKASGHASPNDLKLMINLLRPTYFVPV
                     QGEYRMQAAHAQLANEVGIPFKNIFIPGKGDVIEYAKGRMHMTGQVPAGNVLIDGIGV
                     GDIGNIVLRDRKLLSEDGIFVVVVTISRRLNKILSGPEIVSRGFVYMKASEELVKESS
                     NIVREVVEDNLHTKDFDWAKLKQEIRDSLSRYLFEKTKRKPVILPIIMEASNYQKKS"
     gene            1587..2189
                     /locus_tag="PLT73_01685"
     CDS             1587..2189
                     /locus_tag="PLT73_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRM19124.1"
                     /translation="MGSNSTKAWIEAALFAVLAIALAYVAMPFGEYTIVFALLPLLFI
                     SLRRGILLGLVSGILTGLVLFALKGEGADVAADILNQAAPFVFVGIAGFFAKFTQRTL
                     NNKRFPNAALNIVTASFFGTLVYFVWVLISDIFLSEEAVPAGVSAFAHFLPGQALSFA
                     ATFAVSAVVLVLIAKFAPKAYIPKGSRFLSRKEKSKLLND"
     gene            2310..>2613
                     /locus_tag="PLT73_01690"
     CDS             2310..>2613
                     /locus_tag="PLT73_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRM19125.1"
                     /translation="MGIWSMIFTAIIVLNTIGAIITVFKEKRDVAATWAWLLTLNLLP
                     VAGFIIYLFIGKKISKENIYDMRTQKSLGMSQLAKVQIEMLEDEDLVSELSVYAWES"
     assembly_gap    2614..2623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2624..3810
                     /gene="cls"
                     /locus_tag="PLT73_01695"
     CDS             <2624..3810
                     /gene="cls"
                     /locus_tag="PLT73_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017868314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRM19126.1"
                     /translation="DLAQGLVETDYAQKTAILFLESDESILTKGNKVEIFKTGADLFD
                     SLVQDIYKAENHVHMLYYTFRSDDLGKRILAALEDRAAAGVEVLVVYDAMGCRGNDPR
                     FFNNLEKLGGKTEVFFGSKIPFVNLRMNYRNHRKIMVVDAKVAYLGGFNIGDEYLGKG
                     ELGEWRDTHMKIEGNAVLTLQSRFFMDWNAVAKEKNRKDYAEAYFPIADKKGNTAMQI
                     VASGPDDEDQTIKMGFLKMISQAKESIYIQTPYFIPDESLHEMLKIAVLSGVKVKIMI
                     PSKPDHPFVYRATEYFAKDIVEYGAEVYMYQNGFLHSKVMVVDDEIVSVGTANMDVRS
                     FKLNFEINAFIYDRDVAEELIANFDEDQKNCIRATHDYFAKQSKWRKFKQAFSRLLSP
                     IL"
     gene            complement(4018..4362)
                     /locus_tag="PLT73_01700"
     CDS             complement(4018..4362)
                     /locus_tag="PLT73_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19127.1"
                     /translation="MQNYIYPVSTNNENPSNKSYLRPLSSVHSINEAILDKVMPIHQV
                     IRFANQAFENNNPVYVVIEEKHGPKSYTRSLVWGSFNSRVTTKRQIMFTTADKKVTYL
                     LNISQVLAIQLG"
     gene            4897..5217
                     /locus_tag="PLT73_01705"
     CDS             4897..5217
                     /locus_tag="PLT73_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19128.1"
                     /translation="MQIFDEVKKIEQEAADLELDYQNKLRKLEQNTQDKIGEMKRNIE
                     KELATFQSEELAKKSEQLAILKNASAESEKSEIEKLQTRFKAKEEELVNAVIGEVMRK
                     YGNR"
     gene            5204..>6997
                     /locus_tag="PLT73_01710"
     CDS             5204..>6997
                     /locus_tag="PLT73_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HRM19129.1"
                     /translation="MAIAKMKKVTLISFHEKKEQLLTAIQTLQSLEVIDLPAAKENLQ
                     SLSPKERESIEQNIKKTESYLENVTEALSFLKSYLKKESLIKQYTNPRQSLSLEELEK
                     AVGEFSSDKVLDKVLGLKAEQKKLEDDKKHLYEQEDFLLLWKKLSFNPRDTEAFEHIA
                     VKVGTIPQTLSNDYITNVTSNPLVYTEEIFQNKESFGAVIFYDPSHVIDVNALLDSNH
                     FQALTYEYDLPPAEALADVQSRIQDNREANKQLLADLSEMKAEEWQLMLLSEFYYAKL
                     QRIKSQQLLINEKHLFVIQGWLEEDKVNEIKLALTDTVAENEYALTVDDADTSDISVI
                     PTVLKNNKLITPFENITAMYSLPKYDEIDPTPLLTPFYLLFFGMMIADVGYGLLLLIG
                     TLLALKFLNFNKGMRTNLLFFHILSYPAILWGLVYGSFFGVQLPFVLLSTSNDANTIL
                     LISVVFGIVQIMFGLGIKAYLSLRDKNPLGAISDSIGWLGIFVGLIFILFGQMLFPSQ
                     ILVTVGAAVAITSAVAIVLATAMASDNKALGAGAGLYNLYGITGYIGDMVSYTRLMAL
                     GVAGGSIAVAFNMIIDFLPVGARFTIGAVLFV"
BASE COUNT         2017 a         1335 c         1690 g         1945 t
ORIGIN      
        1 ttacttacct ggaggaaaac aaaaataaga tagcgggtat tttcctgacg catggtcatg
       61 cggatgccgt aggagcattg ccttatttgt tgcagaatat cgatgcaccc gtgttcggta
      121 caggattgac gattgcgttg gctaaattgg cggtcgattc caccggattg gctacgggat
      181 ttgaggatta tcacgttatc gatgagaaca ccgagattga attcgaaaac accagtgtcc
      241 ggttcttcag aaccacgcat accatccctg attctgtagg gatctgtgtg aagacggaag
      301 aaggcagtgt cgtctataca ggtgatttca agtttgatca aagtgcatcc ccgatgtatc
      361 ggaccgatta cggcaaaatt acggatatcg gagaaggcaa agtattggcg ctgttgagcg
      421 attccggtga tgccgaatcg actacggaaa acgtcagtga ccgcaagatt gaagaagaaa
      481 tgctggatac tttcatcaac gctgaaggcc gtatcatcgt ctcctgtgtt gcaagcaaca
      541 ttttgcgcat ccagcaagtt tttgatgtcg ctctccaagc gaatcgtaaa atctttttga
      601 caggaccgac tttggttgaa acagtcgatg tcgccatgaa actggggaaa ctggtcttgc
      661 cgactccaga tctgcttgtg aatgtgaaga acatcaatga ttaccgtgat ggtcaagtgt
      721 tgattctgga aaccggcaac agtggggaac ccttggaagc tttgcagcgg atgtccaaag
      781 gcagacacaa gcaagtgaac cttaaagagg gtgatttggt ttacatcacg actacacctt
      841 caacagctat ggaaacgaca gtcgcaaaaa ccaaaaattt gatttatcgt gccggtgcgt
      901 ccgtgcgtga aatatccgga acatacaaag catccggaca tgcttcgccg aacgatctga
      961 aattgatgat caacctactg agacctactt atttcgtgcc tgtccaaggt gagtaccgaa
     1021 tgcaagctgc acatgcccaa ttggcgaatg aagtcggcat tccgttcaaa aatattttca
     1081 tccctggtaa aggtgacgtg atcgaatatg ccaaaggccg tatgcacatg acgggacagg
     1141 tcccagccgg gaatgttctg atcgatggga tcggggttgg cgatatcggc aacattgtct
     1201 tgcgcgaccg caaactatta tcggaggatg gcatcttcgt tgtcgttgtg acgatctcca
     1261 gaagactgaa caaaatcttg tcgggtccgg aaatcgtttc gcgtggcttc gtctatatga
     1321 aggcgagcga agagcttgtc aaagaaagtt cgaatatcgt ccgggaagta gtggaagata
     1381 acctgcacac gaaagacttt gattgggcca agttaaaaca agagatacgt gattcgttga
     1441 gccgttatct ttttgaaaag acgaagcgca aaccggtcat tttaccgatc atcatggaag
     1501 catccaatta tcagaagaag agctgatgcc cgtttgccgt aaaggcaatg aaacggatcg
     1561 ttgttgaaaa ggagagattc atctttatgg gttctaatag tacgaaggct tggatcgagg
     1621 cagctctgtt tgctgtcctc gcaatcgcct tggcatatgt ggccatgcca tttggagaat
     1681 acactattgt ttttgctttg ttgccgttgc ttttcatttc tctccgcaga ggaatattgc
     1741 ttggactggt ttctggaata ctgaccggcc tcgttttgtt tgcgctcaaa ggagagggag
     1801 cggatgttgc tgcagatatc ctgaaccaag ccgcaccgtt cgttttcgtg ggcatcgccg
     1861 gttttttcgc gaaattcacg caacgcacat tgaacaataa acgtttcccg aatgcagcct
     1921 tgaatatagt gacggcatcc tttttcggaa cgcttgtcta ttttgtctgg gtgctgatct
     1981 cggacatatt cctcagtgag gaagcagttc cagctggtgt ttctgctttt gcgcattttc
     2041 ttccgggaca agcgctatcg tttgcggcaa cttttgccgt cagcgcagtc gttctggtgt
     2101 tgattgcaaa attcgctcct aaagcgtaca ttccaaaagg cagccgtttc ctgagcagaa
     2161 aagaaaaatc gaaactatta aatgattaaa aaatagaagg cgaaggacgg atttgaatta
     2221 tcaggtcttc gcctttctgt ttttgtgggc tcgtgtgcta tgatatgtag gtaacatgtt
     2281 ttgtcatttt attgatttgg agtggtctta tgggtatttg gtcaatgata tttacggcaa
     2341 tcatcgtttt aaatacaatt ggtgcaatca tcaccgtctt taaagaaaaa agagatgtcg
     2401 ctgccacatg ggcctggctg ttgacgttaa acctcttgcc ggtggcgggt ttcatcatct
     2461 acctctttat agggaaaaag atatcaaaag aaaacatcta tgatatgcgc acgcaaaaaa
     2521 gcttgggcat gtcccagttg gcgaaggtcc agattgaaat gttggaggat gaggatctgg
     2581 tcagcgagct ctccgtgtat gcgtgggaga gcgnnnnnnn nnnaggatct ggcgcaaggt
     2641 ttggtcgaaa ctgactatgc acaaaaaaca gctatcctgt tcctggaaag tgatgaatcc
     2701 attttgacga aagggaataa agttgaaata ttcaaaactg gcgcagatct tttcgattcc
     2761 cttgtgcagg acatctataa agcagaaaat catgtgcata tgctttatta tacttttcgc
     2821 tcggacgact tggggaagcg gattctggcg gcgttggaag acagggcggc ggctggcgtt
     2881 gaggttctgg tcgtttacga tgcaatgggc tgcagaggga atgatccgag atttttcaat
     2941 aatcttgaaa aattaggcgg aaagacggaa gtattttttg gttcaaaaat acctttcgtt
     3001 aatttgcgga tgaactatcg gaatcatcgc aaaataatgg tagtggatgc aaaagttgct
     3061 tatctcggcg ggttcaatat aggcgatgag tatctaggga aaggggaact cggcgaatgg
     3121 agggatacgc atatgaaaat agaaggcaat gcagtcttga cgcttcagag ccgtttcttc
     3181 atggactgga atgcggttgc caaagaaaag aacaggaaag attatgcaga agcctatttc
     3241 ccgatcgccg ataaaaaagg taacacggcc atgcaaattg tggcaagtgg accggatgac
     3301 gaagatcaga cgatcaagat gggtttcttg aagatgatca gccaagctaa agaatcgatt
     3361 tatatacaga ctccttattt tatacctgac gagagtctgc atgaaatgct caaaatcgca
     3421 gttctatccg gtgtaaaagt caaaatcatg ataccctcga agccggatca tcctttcgtc
     3481 tatcgggcaa cggagtattt tgccaaagac atcgtcgagt acggtgcgga agtctatatg
     3541 taccagaatg gtttcctgca ttcaaaggtg atggtggtcg atgatgaaat tgtatcggta
     3601 ggcacggcaa atatggatgt gcgcagtttc aaattgaatt ttgagatcaa cgcttttatt
     3661 tacgatcgtg acgtagctga agaattaatc gcaaattttg atgaagatca gaagaattgc
     3721 atccgtgcaa cccatgatta tttcgcaaaa cagtcgaagt ggcggaaatt caagcaagct
     3781 ttttccagat tactttcacc gatactgtag accgaaagcg tatggaaata gatcgacaat
     3841 cagaattcag aagaaaggcc cacaagcagt caaaacaagc gagaatgcaa ttgtgtgaga
     3901 tgcattctcg cttgtttcag ttgtcatagt tttttacgtt aatgacttta ccttgtgctc
     3961 ctaatttcta tagaagggta tgccaccaaa gaagggtatg ccaccaactg attgaattta
     4021 accaagttgg atagccaaga cttgactgat gttcagtaga taagtcactt ttttatcggc
     4081 agttgtgaac atgatttgtc tttttgtagt tacgcgggaa ttgaaagaac cccaaacgag
     4141 tgatctggta tacgatttcg gtccatgctt ttcttcaatc acgacataca cagggttgtt
     4201 attttcaaaa gcctggttag caaaacggat aacttgatga atcggcatta ctttgtccaa
     4261 gatggcttca ttgatggaat ggacggatga gagcgggcgc aagtatgatt tatttgaagg
     4321 attctcatta ttagtggata ctgggtagat gtagttttgc atgtatgttc ctccgaacgt
     4381 gtgtttgtct tgtgtcttta ttataagaac atctgttctt gtttgcaagc tttttatttt
     4441 gcgatcgaac aaaaatttga gagtgcagat atgtaactcc acccacaatt atgtcatatc
     4501 ggccgaatat gcccctattt cttgcatttt cgagtaaagt agtagtatgg aaaagaacgt
     4561 gtcgggagca cattaatttt gcaggcgagg ggaaaattac atctattatc cggcggaatt
     4621 atcgaaaaaa ataggctata taaaagactc tccaagaagg ttctggatcg gtgcagcgtg
     4681 cttggagagt gctgttcaca aaatgttcat ttaatatttt tgaagaaatt tcagaaagtt
     4741 tcttcctatt aatgaaactg taatgattgt tgaaaccact gggaattgcg tataaataga
     4801 tttgaacggc ctttacatta actgtataat gaaaatactg accggaaagt ttttttccta
     4861 aggagaatca aatgaaggac ggaggtggaa tgaaagatgc aaatatttga tgaagtaaaa
     4921 aagattgagc aggaagcagc tgatctggaa ttggattacc agaataagct acgcaaactg
     4981 gaacagaaca cgcaagataa aataggcgaa atgaaaagga atatcgaaaa ggaattagct
     5041 acttttcaat ccgaagaact tgcgaaaaaa tcagaacaac tggcaattct taagaacgct
     5101 tccgcagaaa gtgaaaaatc ggaaatcgag aagttgcaaa ctcggtttaa agcgaaagaa
     5161 gaagaacttg tgaatgccgt aattggggag gtgatgagga aatatggcaa tcgctaaaat
     5221 gaaaaaagtt actctcattt cttttcacga aaaaaaagag cagttattga cggctatcca
     5281 gacattgcaa agcttagaag tcatcgattt gcctgctgca aaagaaaatt tacaatcctt
     5341 atcgcctaaa gaacgagagt caattgagca aaacatcaag aaaaccgaaa gttatctgga
     5401 aaatgtcacg gaagctttat ctttcctgaa gtcgtatctg aagaaagagt ctttgatcaa
     5461 acagtataca aatcctagac aatccttgtc tttggaagaa ctggaaaagg ctgtgggcga
     5521 attttcttcc gacaaagttc tggataaagt gttgggtctg aaagccgaac agaagaaact
     5581 cgaagatgat aagaaacatc tttatgaaca agaggatttc ctgcttttat ggaaaaaatt
     5641 atcattcaat ccaagagata cagaagcatt tgaacatatc gcggtcaaag tgggcacgat
     5701 tccccagacg ctttccaatg attacatcac aaacgtaact tccaatccct tggtctatac
     5761 ggaagaaatc ttccagaaca aagaatcttt tggagcggtg attttctatg atccgtcaca
     5821 tgttattgat gtaaacgctt tgttggacag caatcatttc caagctttaa catatgagta
     5881 tgatttgccg cctgcagagg cattggccga tgttcaaagc cgcatacagg ataaccgtga
     5941 agcaaacaaa cagctgttgg cggatctttc cgaaatgaaa gcagaagagt ggcagttgat
     6001 gctactgtcc gaattttatt atgcaaaact gcaacggatc aagagccaac aattattgat
     6061 caatgagaaa catctcttcg taatccaagg atggcttgag gaagataaag tcaatgaaat
     6121 caagctggcg ttgactgata ctgtcgcaga gaatgaatat gcattgacgg ttgatgatgc
     6181 tgacacttct gacatttcgg tcattccaac cgttttgaag aacaataagt tgatcactcc
     6241 ttttgagaat atcacggcca tgtacagttt gccgaaatat gacgaaatag accccacacc
     6301 actattgaca cctttctatc ttttgttctt cggaatgatg attgcggatg tcgggtacgg
     6361 tctgttgctc cttatcggaa cattgctggc attgaagttc ctgaacttca ataaaggtat
     6421 gcgcaccaat cttctgttct ttcacatcct ttcctatcca gccatactgt gggggctagt
     6481 ttacggctct ttcttcggag ttcaattgcc gtttgtactt ttatctacga gtaatgacgc
     6541 caatacgatc ctgttgatat cggtagtatt cggtattgtt cagatcatgt tcggtttggg
     6601 gataaaagcc tatctgtcct tgcgcgacaa aaatccgctg ggcgcgattt ccgacagcat
     6661 cggatggttg ggtatatttg tgggacttat cttcatcctg ttcggacaga tgcttttccc
     6721 tagtcagatt cttgtgaccg ttggtgctgc agttgccatc acaagtgcgg tcgcgatcgt
     6781 gcttgctaca gccatggcat cagacaacaa ggcgttgggc gcaggtgccg gtttgtacaa
     6841 tctctatggc attaccggtt acatcggcga catggtcagt tacacacgtt tgatggcgct
     6901 gggtgtggct ggtggcagta ttgcggttgc cttcaatatg atcatcgact tcttgccagt
     6961 gggggcaaga ttcaccattg gcgcggtttt gtttgtg
//
LOCUS       DAOMUJ010000035         6913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2912_length_6913_cov_3.129046, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000035 DAOMUJ010000000
VERSION     DAOMUJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_6913_cov_3.129046"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1633
                     /gene="gtfA"
                     /locus_tag="PLT73_01715"
     CDS             194..1633
                     /gene="gtfA"
                     /locus_tag="PLT73_01715"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HRM19130.1"
                     /translation="MKIKNEAMLITYPDSLGNNLQDLEQVLDTHLKGVVGGVHILPFF
                     PSSGDRGFSPMDYTKVDERFGGWEDIKRISEKYYMMYEFMLNHISAQSPYYLDFLEKK
                     EASPYKDYFIRYNDYWPENRPTEADIDLIYKRKPKAPFVDAHFKDGTSEKVWCTFSEE
                     QIDLNVKTEATRQFIKDTLSFLADKGASIIRLDAFAYAIKELDTNCFFVEPEIWEMLE
                     YAVEILKPYGVTVLPEIHEHYTIQQKIAEKGYPVYDFALPMLVLHALYSGKAEKLLHW
                     LEICPRNQFTTLDTHDGIGVVDVKDLLTQEEVDFAVEALYEKGANLKRIYSSEAYNNL
                     DIYQINCTYYSALGNNDAAYLLARAIQCFTPGIPQIYYVGLLAGENDLELLENSKEGR
                     NINRHYYSLDEINQEIERPVVKDLFRLLAFRNTAKAFDGDLKITMIDEGAFTLTWATA
                     EESASLTVDLPTNKFSILHRTAAGDEQIF"
     gene            1658..3865
                     /locus_tag="PLT73_01720"
     CDS             1658..3865
                     /locus_tag="PLT73_01720"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM19131.1"
                     /translation="MTVTITFDETKKYFHLQNESVSYVIALEEEKYVSHQYWGKRLNS
                     FSQVADYPRQDNAFAPNPFEVPGRVFSLATLPQEFPGNGSGDFRESAFECLYPDQTTV
                     SLLTYKSHEIVTGKQPLQGLPHTYETKEEPAETLLLTMEDTVTKVEVVLSYTLFRDYP
                     VLTRSASFRNNGAEDIHLNKALSMSIDFPDADFDMIQLPGAWGRERQIVRTPLVRGIH
                     TLDSKRGTTSHAYQPFVALADKTATEDQGAVYGFHFVYSGEFRANVEVDTYAQTRVQM
                     GINPTHFQWHLPVGEAFQTPEVVLVYSENGLNGLSQTLHPFYQKHLIRGQHQLAERPV
                     LINNWEGTYFDFTAEKIEAMADEASTLGIELFVLDDGWFGKRDDDYSSLGDWHVHTAK
                     LPSGLKQLAENIKAKGMLFGLWFEPEMISEDSELFRAHPDWCIHTPGREKSLSRSQYV
                     IDFSRKEVRDNILAQMMAILDDVPVDYIKWDYNRNMTEIGTAAPGALPGEVLHKYMLG
                     LYEVMEALVTKYPQILFESCSGGGGRFDPGILYYMPQTWTSDNTDAVARLEIQYGTSL
                     VMPISSMGSHVSAIPNHQTHRKASMKMRGDVAMAGNLGYELDVTTLSEAEKQEMKEQI
                     SFYKEHRKLVQYGVFHRILSPFETQNEAAWIFVSPEQDEALYFYYRVLDRANLKKKKV
                     LFKGLDSAKYYTVSGYEGLIGGDELMNRGLYLKDALQGDYQSVCLVLKEAQSV"
     gene            complement(3923..4912)
                     /locus_tag="PLT73_01725"
     CDS             complement(3923..4912)
                     /locus_tag="PLT73_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19132.1"
                     /translation="MNTLRGIHHVTAITSSAEKNYAFFTNILGMRLVKKTVNQDDIQT
                     YHLFFADDRGSAGTDITFFDFPGIPKAVRGTNEIFKTGLRVPSDEALVYWVKRFDKYN
                     ITHSGIYEQFGRKVIDFQDFDEQLYQLVSDEADTGVAPGIPWQKGPVPNEYGIRGLGP
                     IFVRIADFNFYRQVLETVLGFRHADSVGETHLFEVGEGGNGGRMIVEHNATLGQAQQG
                     FGSVHHMAFRVKDRKELEEWIAHMGSYRFPISGYVDRFYFESLYANIAQGILLEFATD
                     GPGFIDDEEPYETLGERLALPPKFRNNREEIEGLVRQFDTVRSTKTFEKEYLE"
     gene            5179..6669
                     /locus_tag="PLT73_01730"
     CDS             5179..6669
                     /locus_tag="PLT73_01730"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HRM19133.1"
                     /translation="MAGNGLMMQYFEWYLDDDGQLWNRLKEDAKHLKELGITAVWTPP
                     AYKATNDTGYGVYDLYDLGEFDQKGAVRTKYGTKAEYLAAIEALHTEGIAVYADVVLN
                     HKAGADETERFLAYEVNPDKRQEKESKSYEIEGWTKFTFPGRGDKYSDFKWSWEHFTG
                     TDFNNENGKEAIYMIKGFEKGWAENESVDSEYGNYDYLMYADIDYGHPAVVEEVKKWA
                     DWYIKETGVDGFRLDAVKHINDQFVKDLVETIRAQHGDDFYVVGEYWKYKYGAIKEYL
                     EATEFTFDLFDVALHQNFHVASQQGKDYDLRELFKQTLVAKNPTHAVTFVDNHDSQPD
                     QALQSFVEPWFKPLAYGVTLLREQGFPCLFYGDYYGIKGANPIDGQKTFLDKLLYLRA
                     NHAYGEQRDYFDHGNCVGWTRLGNEEHPYGLATVLSNSEEGFKDMYVGEQYAGQTFAD
                     YTGNREDKVEIGEDGNGRFPVNAGSISVWVKDAISPEEAFDADAVE"
     gene            complement(6710..>6913)
                     /locus_tag="PLT73_01735"
     CDS             complement(6710..>6913)
                     /locus_tag="PLT73_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19134.1"
                     /translation="VGRRRAAECKRSSGEGPSNRSRPAIVAFNYDEAAFFGEHKQNVG
                     RTPAKPSFTGGERSLPTMKEVPP"
BASE COUNT         1815 a         1711 c         1827 g         1560 t
ORIGIN      
        1 aatatgctga aagacttcct gcatcagcag gttagtcgaa cagtatcctt ggctcgtaga
       61 gacaaggggt tccttgatcc tttccgaagg gctaacccgt gaagctagac atcattagaa
      121 aagcggacca agcccgctag acatcaccaa taaaagacgt aacttaacca tacttgaaaa
      181 tgaggtaact gtaatgaaaa tcaaaaacga agcaatgctc atcacctatc cggacagtct
      241 gggcaataac ctgcaagatc tggaacaggt gctggatacc catctgaaag gggtcgtcgg
      301 cggcgtgcat atcctgccgt tcttcccttc atcgggcgac cgcggctttt cgccgatgga
      361 ttacacgaaa gtggatgagc gttttggtgg ttgggaagat atcaagcgca tcagcgaaaa
      421 atactacatg atgtatgagt tcatgctcaa ccacatttct gcgcaatctc cctattattt
      481 ggactttttg gagaagaaag aagcatcgcc ctacaaggac tacttcatcc gctacaacga
      541 ctattggccg gagaaccgac cgactgaagc ggacatcgat ctgatctaca aacgcaaacc
      601 caaagcgcct ttcgtggatg ctcacttcaa agacggcaca tcggaaaaag tctggtgcac
      661 gttctcggaa gagcagatcg atctgaacgt gaagaccgaa gcgacgcgcc agttcatcaa
      721 ggatacgctt agtttcttgg cggataaagg agcttcgatc atccgcctgg atgcattcgc
      781 ctatgccatc aaggaattgg atacgaactg cttcttcgta gaacccgaaa tttgggagat
      841 gctggaatac gcagtggaaa tcttgaagcc ctatggtgtg acggtccttc ccgaaatcca
      901 tgagcattat acgatccaac aaaaaatcgc agaaaaaggc tacccggtct atgatttcgc
      961 tttgccgatg ctggtgctgc acgctcttta cagcggcaaa gcggaaaaac tcttgcactg
     1021 gttggaaatc tgcccgcgca accagttcac gaccttggat acgcatgacg gcatcggtgt
     1081 cgtcgacgtg aaggatctgc ttacccagga agaagtggat ttcgcagttg aagcccttta
     1141 tgaaaagggc gccaacctga agcggatcta cagctcggaa gcctacaaca acctggacat
     1201 ctatcagatc aactgcacct actattcggc tttgggcaac aatgatgccg cctatctgtt
     1261 ggcgcgggcg atccagtgct tcacgccggg cattccgcag atctattacg tgggcttgct
     1321 ggccggcgaa aacgatctgg aactgctgga gaacagcaaa gaaggccgca acatcaaccg
     1381 tcactactat agcctggatg aaattaatca agaaatcgag cgtccggtcg taaaagatct
     1441 gttccgcttg ctggctttcc gcaatacagc caaagcgttc gatggcgact tgaaaataac
     1501 gatgatcgac gaaggggcct tcactttgac gtgggccact gccgaagaat cggccagtct
     1561 gacggtcgac ctgcctacga acaaattctc gatcctgcac cgcactgcgg caggagatga
     1621 acaaatattt tagaaaaatg atgagagcca aggagtgatg actgtgacga taacatttga
     1681 tgaaacaaaa aaatattttc atttacagaa cgaatcagtg agctatgtga tcgcactgga
     1741 agaagaaaaa tatgtgtccc atcagtattg gggaaagcgg ctcaacagct tttcgcaagt
     1801 ggctgattac ccgcgccaag acaatgcctt tgcgcccaat ccgttcgagg tgcccggccg
     1861 tgtgttttcg cttgcgacct tgccacagga atttccgggg aatggcagtg gcgatttccg
     1921 cgaatcagca ttcgagtgcc tgtatccgga tcaaacgact gtcagcctgc tgacttataa
     1981 gagccatgaa atcgtaaccg gcaaacagcc attgcaaggc ttgccgcata cttacgaaac
     2041 aaaagaagag ccggcggaaa ccttgctgct gacgatggaa gataccgtca caaaagtcga
     2101 agtcgttctg agctacacct tgttccgcga ctatccggta ctcacacgtt cagcaagttt
     2161 ccgcaacaat ggagctgaag acatccatct gaacaaagcg ttgagcatgt ccatcgattt
     2221 cccggatgct gattttgaca tgatccagtt gcctggtgct tggggaaggg aacgccaaat
     2281 tgtgcgtacg ccacttgtcc gcggcatcca cacgcttgac agtaagcgtg gcactaccag
     2341 tcacgcctat caaccatttg tggcgttggc cgataaaacg gcgaccgaag accagggagc
     2401 ggtctacggt ttccatttcg tctacagcgg cgaattccga gccaatgtcg aagtcgatac
     2461 ttacgcgcaa acacgcgtcc agatgggcat caatccaacg cacttccaat ggcatttgcc
     2521 tgttggagag gcgttccaga caccggaagt cgtattggta tacagcgaaa acgggttgaa
     2581 tggcctatcg cagacgctgc atcctttcta ccagaagcat ctgatccgcg gccagcacca
     2641 acttgcggag cgtccggtcc tgatcaacaa ctgggaaggc acctattttg atttcacagc
     2701 cgaaaagatt gaggcgatgg ccgatgaagc atccactttg gggatcgaac tgttcgtgct
     2761 ggacgatggt tggttcggca aacgtgacga tgactactct tcgttgggtg attggcatgt
     2821 ccataccgcg aaattgccgt ccggcttgaa gcagctcgcg gagaacatca aggcgaaagg
     2881 gatgctgttc gggctttggt tcgaaccgga aatgatttcc gaggatagcg aacttttccg
     2941 tgcccatccg gattggtgca tccatacgcc aggccgggaa aaatcgctca gccgcagcca
     3001 atacgtgatc gacttcagcc gcaaggaagt ccgcgacaac atcctggcgc agatgatggc
     3061 gatactggat gacgtcccgg tcgactacat caaatgggac tataaccgca acatgaccga
     3121 aatcggtacg gcggctccag gcgcattgcc gggggaagtg ctgcacaagt acatgctggg
     3181 tctttatgaa gtgatggagg cactggtgac gaaatatccg cagattctct tcgaaagctg
     3241 ctcaggcggt ggcggacgct tcgacccagg tatcctttac tatatgccgc agacttggac
     3301 cagtgacaat accgatgcgg tggcgagact ggaaatccaa tacggcacca gccttgtcat
     3361 gccgatttcg agcatgggtt cgcacgtctc agctatcccg aatcatcaga cgcaccgtaa
     3421 ggcttcgatg aagatgcgcg gggatgtggc gatggccggc aatcttggct acgaactcga
     3481 cgtcacgacg ctttccgaag ccgagaagca ggagatgaag gagcagatca gcttctacaa
     3541 ggaacaccgc aagctcgtcc aatacggtgt cttccatcgc atcctcagtc ctttcgaaac
     3601 gcagaacgaa gcggcttgga tcttcgtcag tccggaacag gatgaggcgc tttatttcta
     3661 ctaccgcgtc ctcgatcggg ccaacctgaa gaagaaaaaa gtgttgttca aaggcttgga
     3721 ttctgccaaa tactacaccg tcagcggcta cgaaggtcta atcggcggcg atgaattgat
     3781 gaaccgcgga ctctacctga aggatgccct gcaaggggac taccagagtg tctgtctggt
     3841 gctgaaggaa gcacaatccg tttaaaacgc aaaaaaagca ggagttgtct cgttgattcg
     3901 agacggctcc tgctttttgt tctcattcca aatattcttt ttcgaaagtc ttcgtgctgc
     3961 gcacggtgtc gaattggcgg accaagcctt cgatttcttc gcgattgttg cggaatttcg
     4021 gcggcaaggc caggcgttcg cccaatgtct cgtaaggctc ttcatcatcg atgaaccccg
     4081 ggccgtccgt cgcaaattct aacaggatgc cctgagcgat gttggcgtag agcgattcga
     4141 agtagaaacg gtcgacatag ccggaaatcg ggaagcggta agagcccata tgcgcgatcc
     4201 attcttccaa ttctttgcgg tccttcacgc ggaaagccat gtggtggacg ctgccgaaac
     4261 cttgctgcgc ctgacccagc gtagcgttgt gctccacgat catgcggccg ccgtttccgc
     4321 cttcgcccac ttcgaagaga tgtgtttcgc cgactgaatc agcatgacgg aaaccaagga
     4381 cagtctccaa gacttgtctg tagaaattga aatcagcgat gcggacgaaa atcggtccca
     4441 aaccgcggat gccgtactca ttcggaacag gccctttttg ccacggaatc ccgggagcca
     4501 cgccggtatc agcctcatcg gaaaccaatt ggtaaagctg ctcatcgaaa tcctggaagt
     4561 cgatcacttt gcggccgaac tgctcataga tgccggaatg tgtgatgttg tatttgtcga
     4621 aacgttttac ccaatagacc agtgcttcat ccgatgggac gcgcaggccc gtcttgaaga
     4681 tttcattggt ccctctgact gctttcggga tcccagggaa gtcgaagaag gtgatgtccg
     4741 tgccggcact cccgcggtca tcggcgaaga ataggtgata agtctggatg tcgtcctggt
     4801 tgacggtttt tttgaccaag cgcataccca gaatattggt gaagaaggca taattcttct
     4861 cggcgctgct ggtgatggcg gtgacgtggt ggattcctct taaagtgttc atggatatta
     4921 tcctcatttc gttatgtatt ctgtttcgta tattcctaat tcgagatata tagccaaaaa
     4981 aagaaatcag caatcagcta acttaaggtc agtatacagg cgtccgattc aagatgcaag
     5041 cattttgccc tgccaagaag aaacggcacg cgggaggcgt ctcaaaagga aaatggaagg
     5101 gaagcgtgta taatgaggat agcaaaaagc attttgtgga ccacaacaaa attaaacagg
     5161 atgaaaggca gtgatcgcat ggcaggaaat ggattgatga tgcagtattt tgaatggtat
     5221 ctggatgatg atggacaact atggaatcgc ctgaaggagg atgccaagca tctgaaggaa
     5281 ctgggcataa cagccgtctg gacgcctcca gcctataaag cgacgaatga tacaggctat
     5341 ggcgtctatg atctctatga tttgggcgaa ttcgatcaaa aaggtgccgt ccgcacaaaa
     5401 tacggaacca aagcagaata cttggctgcg atcgaagctt tgcatacgga aggaattgct
     5461 gtttatgccg acgtcgtcct gaaccacaag gccggtgcag acgagacaga acgcttcctt
     5521 gcttatgaag tgaatcccga caagcgtcag gaaaaggagt ccaagtcata cgaaatcgaa
     5581 ggttggacga aattcacttt tccgggccgt ggcgacaaat attctgattt caaatggtct
     5641 tgggaacatt tcaccgggac ggacttcaac aacgagaatg gcaaagaagc tatctacatg
     5701 atcaaaggct tcgaaaaagg ctgggcggaa aacgaaagtg tagacagcga gtacggtaat
     5761 tacgattact tgatgtatgc ggatatcgac tacggccacc ctgcagtcgt tgaggaagtc
     5821 aagaaatggg ccgattggta catcaaagaa accggtgtcg acggcttccg cttggatgcc
     5881 gtcaaacaca tcaatgatca gttcgtgaag gatctcgtgg agacgatccg cgcgcagcat
     5941 ggcgatgatt tttacgtggt cggtgaatat tggaagtaca aatacggcgc gatcaaggag
     6001 tatttggaag cgaccgagtt caccttcgat cttttcgatg tggccctgca tcagaacttc
     6061 catgtcgcat cccagcaggg caaggattac gatctgcgcg agttgttcaa gcagacgctg
     6121 gtcgccaaaa atccgaccca tgcggttacg tttgtcgaca accatgactc acaacccgac
     6181 caagctttgc aatcctttgt ggagccatgg ttcaaaccgt tggcatacgg cgtgacactc
     6241 ttgcgcgaac aaggcttccc gtgcttgttc tacggcgatt attacggtat taaaggtgcg
     6301 aatccaatcg acggacagaa aaccttcctg gataaattgc tctatctgcg cgcgaaccat
     6361 gcttacggtg aacagcgcga ctacttcgat catggaaact gcgtcggttg gactcgtctg
     6421 ggcaacgaag agcatccgta cggtttggcg acagtccttt ccaacagtga agagggcttc
     6481 aaggacatgt atgtcggcga acagtatgcc gggcagactt tcgcggacta tacaggcaac
     6541 cgtgaagaca aagtcgaaat cggtgaagac ggcaacggcc gcttcccggt caatgccggt
     6601 tcgatttcgg tatgggtcaa agacgcgatc tccccagagg aagcattcga cgcggatgca
     6661 gtcgaataga ttgaagcgaa aataatagct gcacaatgaa ggaggtgtct catggaggca
     6721 cctccttcat tgttggtaga gaacgctctc ctcccgtgaa cgaaggcttc gccggagttc
     6781 ggcccacatt ctgcttgtgt tctccgaaga aggcagcttc gtcgtagttg aatgcgacta
     6841 ttgccggcct gctccgattg gaaggtcctt cgccggagct gcgcttgcat tccgccgccc
     6901 tcctccggcc aac
//
LOCUS       DAOMUJ010000036         6840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_2954_length_6840_cov_2.562859, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000036 DAOMUJ010000000
VERSION     DAOMUJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_6840_cov_2.562859"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..827
                     /locus_tag="PLT73_01740"
     CDS             264..827
                     /locus_tag="PLT73_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HRM19135.1"
                     /translation="MTEKRVFRSDTFVNRSFVAGIPFILMFGGLTHFAFAWLGKTNWA
                     APFVPVNESVWEHLKMSYWTTFLWFFFIFLFFGKRYRLSSSRWLLAGIVSMLFCPLFI
                     VTGFYTLKGAFGIESLFTDGLLFIFGIISGQLLASRTYRYVEPHRIRIGMAVALWLIL
                     ALAFVLFSFQPPVLPLFLDTPTGSYGF"
     gene            complement(946..>1854)
                     /locus_tag="PLT73_01745"
     CDS             complement(946..>1854)
                     /locus_tag="PLT73_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRM19136.1"
                     /translation="EEILAFIPGISAICEVDTLQICNIDSTNIAPDIWVLMTDTIREK
                     YEQYDGFVVTHGTDTMAYTAAALSYMIQDSPKPIIITGAQKPINVEITDSKTNLFDSF
                     LYAASDSASGVQIVFNGKVILGTRARKTHSKSFQAFSSINYPYLAIIQDGRVISYITK
                     DKAPAPRFYDILDAKVALFKLTPGVDSEILSYMLERNDAIIIESYGVGGIPSAPEYGY
                     FEVINHWIRKGKTVVMTTQVPNEGSDMEIYKVGHELKSRVNILEAYDMITEAVVCKLM
                     WILGQTQDPARIKELFYTTISNDILY"
     assembly_gap    1855..1864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1991..3649)
                     /locus_tag="PLT73_01750"
     CDS             complement(1991..3649)
                     /locus_tag="PLT73_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM19137.1"
                     /translation="MRRNILFILLGLFIFAGLAPVRIAAPAYSLENGTAVEKSLLELY
                     QLAYINLDDTLAETGKDHITQQTRSVAALSGAQKIPLFSATASNAELVYTTYLQDSGW
                     QPEVQEGAISGGVALPLEAVRVRLTHLAEAGSVSYRTYRQDSGWSGYAKNGEAAGVTG
                     AGEAIEAVQFKLTGALAAQYDLYYRVQTGRFGWLDWAGNGETAGTEGFRYPVEGVEIV
                     LMNKEQAFSGKREQSFIKRVEPVLSYSSYLTDSGWQEPVLNPEQSGSPDSSQSLEGLQ
                     AKLDSQPYPGSISYRAATSENGWFEWTQDGLEAGLPGTGQRIERIEMQLTDELATYYD
                     IYYQVHVPGLGWLDWAKNGETAGTQGYDYDVSAVQMQVVQKDAAAPGETAVAFMKYVP
                     KPPPVPVVPSVPTYPTGPDWTVQDGIFRTNAGTNYYVGSSYIIISIAYQRVWAYIGSQ
                     RIVDAPVITGRPSMPTPRGLFAIQPYKESPSVLVGEDYESPVQYWIPFLGNAYGLHDA
                     SWQTQGFGDDLYLYLGSHGCVNTPYYAVQTLYNTYSVGTPVVVY"
     gene            complement(3836..6022)
                     /gene="recQ"
                     /locus_tag="PLT73_01755"
     CDS             complement(3836..6022)
                     /gene="recQ"
                     /locus_tag="PLT73_01755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRM19138.1"
                     /translation="MDKELLYGTLKKYFGYDSFREGQEELITNTLQGKDVLGIMPTSG
                     GKSLCYQLPALLLDGMTIVVSPLVSLMKDQVDSLREMGIAAAYLNSTLSREEFLQLMD
                     DMRSGQLKLLYIAPERIDNESFMNALRFVNVPLLVVDEAHCISQWGSDFRPSYQGVAR
                     LYDVFGRRPRVAAFTATATEPVQDDIMIQLRLQQPFRLVTGFDRSNLAFSVEKPADKF
                     KFLVKDLNPKEASIIYCSTRKNVESVQKKLEQKGFAVTLYHAGLPEHERQRNQDDFLF
                     DRKPIMVATNAFGMGIDKSNVRTVYHYNMPKNIESYYQEAGRAGRDGEEARVILLFST
                     QDIIINQLLNSKTNDSEATNKLNKMINYCYTNQCLRRYILEYFGETTAELNCQHCSNC
                     LHRTEQIDITEEAQKILSCVVRVRGRYGVQKIIGILTGSKQKGITDFGLDKLKTYGLM
                     SAYNDGEIREMIGVLIADEYLQVTGDQYPLIQVTPKALEVLKEGKKIGMSYAVETGRG
                     TAKQSGGGLYDTELFDRLRSVRTEMANEHGIPSYIVFSDSTLHEMARNFPTDDHAFLR
                     LSGVGETKLERYGEVFMGVISDYLKEFPDAQAVLQQKQLEQKELAGNPETAEKRPKPA
                     QKNYAGTTFEETYRLYQECKTVEEIIAIRGLARMTIENHFRHLAELSAYEMRLKDFVT
                     DEQAKAIARAIEESDDQHLKPLKEKLGDDYSYFQIGMVLAFRKREQ"
     gene            6293..>6840
                     /gene="selD"
                     /locus_tag="PLT73_01760"
     CDS             6293..>6840
                     /gene="selD"
                     /locus_tag="PLT73_01760"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRM19139.1"
                     /translation="MEQELSNELFICGGCNAKIGPGVLGNLLSQFPKQENPNFLVGFD
                     SSDDAAVFRIDAETAMIQTLDFFPAMVADASLFGEIAAANALSDVFAMGGEVLTAMNI
                     VCWPETKSTDTLGKILAGGFRKVQEAGGVLVGGHSIHDPQPKYGLSVLGRVHPDRIYR
                     NDTCLPGDVLLLTKPLGTGIITT"
BASE COUNT         1488 a         1838 c         1707 g         1797 t
ORIGIN      
        1 gagacaagag gttccttatt cttttttccg aagggctaac ccgtgaagct agccatcatt
       61 tttggatgca tggaacgctt tacaaacatg tgaacacccc acagcatggc agttccaatc
      121 aggaacagcc atgctgtggg gtgtttttgg catgcagctg agttagatga agtcagggcc
      181 aacgcgtggt aaaatgatca cgaaggatgc ttcatccacc tgccatctgc cgattactga
      241 tccgagagaa agaggtcctt gcgatgaccg aaaagagagt tttccgatcc gatacattcg
      301 tcaaccgttc ctttgttgcc ggcatcccgt tcatactgat gttcggcggc ctcacccact
      361 tcgcattcgc atggcttggg aaaaccaact gggcggcccc tttcgtcccc gtgaatgaga
      421 gtgtctggga acacctgaaa atgagttact ggaccacttt cctatggttt ttcttcattt
      481 tccttttttt cggaaagaga taccggcttt cgtcatcccg ctggttgctg gcggggatcg
      541 tgtccatgct gttctgcccg ctgttcatcg tcactggatt ctacacgctc aaaggtgctt
      601 tcggtatcga atcccttttc actgacgggc tgctgttcat tttcggcatc atcagcggtc
      661 aactgcttgc ttcccggacc tatcgatatg tcgaacctca ccgcattcgg atcggaatgg
      721 cagtcgcgct ctggcttatt ctcgcgcttg cttttgtgct gttcagtttc cagcctcccg
      781 tcctgccgct ctttctggat actccgaccg gaagctatgg gttttagatt gcttaaggag
      841 gtaggttatt cggggcaact gcgttcggac cttcccaaaa aaaataacgc ctaaaataca
      901 gaaaagagcg gaaaagccct cgcagtggat ggcttttccg ctcttttaat acaggatatc
      961 gttgctgatc gtcgtataga acagttcctt gatccgggcc ggatcttgcg tctgtcccaa
     1021 gatccacatc agcttgcata cgaccgcttc ggtgatcatg tcgtaggctt ccaaaatatt
     1081 gacccgcgat ttcagttcgt ggccgacttt gtatatttcc atgtcgctgc cctcattcgg
     1141 gacttgtgtc gtcatgacca ccgtcttgcc ttttctgatc cagtggttga tgacctcgaa
     1201 atatccgtat tccggtgctg aaggaatgcc gcccacccca taactttcga tgatgatggc
     1261 atcgttccgc tcaagcatgt acgaaagaat ctccgaatcc actcccggcg tcagcttgaa
     1321 caacgcgacc ttcgcatcca gaatgtcata aaaacggggt gctggtgctt tgtcctttgt
     1381 gatgtaggaa atcacgcgtc catcctggat gatcgccaaa taaggataat tgatgctgga
     1441 aaaagcctgg aagcttttgg aatgcgtctt ccttgcccgt gttccgagga tgaccttccc
     1501 gttgaagacg atttggacac cactggcgga atcggatgct gcgtacagga aactgtcgaa
     1561 gagattcgtc ttcgaatcgg tgatttccac gttgatcggt ttttgggcac cggtaatgat
     1621 gatcggtttt ggggaatcct ggatcatgta ggatagcgcg gcagctgtat aggccattgt
     1681 atcggttcca tgggtaacga caaacccgtc gtattgttca tacttttccc tgatggtgtc
     1741 ggtcatcagc acccagatgt caggcgcgat gttggtgctg tcgatattgc agatctgcag
     1801 cgtatcgacc tcgcatattg ctgagatacc gggaataaaa gccaatatct cttcnnnnnn
     1861 nnnnatcaag tccgttcggc gttattttgg aggcaatcgt accgcccgtt ccgatcatca
     1921 atatcttttt cttcgtcaca ccttcacctg cttgttcatt attttttcca tgcttttccg
     1981 cgtctggtgg ttaatatacc acgaccggcg tgccgacgga ataggtattg tagagcgttt
     2041 ggaccgcata gtacggggta ttgacgcagc cgtgcgatcc taaataaaga tagaggtcgt
     2101 cgccaaagcc ttgggtttgc caagaggcgt catgcaagcc gtaagcattc cccaaaaatg
     2161 ggatccaata ttggacaggt gattcgtaat cttcaccaac cagcacgcta ggtgattcct
     2221 tatacggttg gattgcgaac agccctcttg gcgtcggcat cgaaggcctt ccggttatga
     2281 ccggagcatc gacaatccgc tggctgccga tgtaggccca gacgcgctgg taggcgatgc
     2341 tgatgatgat gtagctggaa ccaacataat agttggtacc ggcgttcgtc cggaagatac
     2401 catcctggac agtccaatcg ggtcccgtcg gataggtcgg gacagatggt acaaccggaa
     2461 caggcggtgg tttcggtacg tatttcatga aagcgactgc cgtctcccct ggtgctgccg
     2521 cgtctttttg gaccacttgc atctggacgg cgctgacgtc atagtcgtag ccttgtgttc
     2581 ctgccgtctc cccgttcttg gcccaatcca gccaacctaa tcccggtaca tggacttggt
     2641 aatagatatc ataatacgta gccagttcat ccgtcagctg catttcgatc cgctcgatac
     2701 gttggcctgt tccgggcagt ccggcttcca aaccatcctg cgtccattca aaccagccgt
     2761 tttcacttgt ggcagcgcgg taggaaatcg atcctggata gggctgggag tccaattttg
     2821 cttgcaggcc ttcgagagat tgcgaggaat ccggagatcc gctttgctcc ggattgagga
     2881 ctggctcctg ccagcccgaa tccgtcagat agctgctgta ggacaagacc ggttcgacgc
     2941 gtttgatgaa gctctgctca cgtttacctg aaaaagcctg ttccttgttc atcagaacga
     3001 tttcaacccc ttcgaccgga tagcggaaac cctccgttcc ggctgtttca ccgtttccgg
     3061 cccaatcgag ccacccgaaa cggcccgttt gcactcggta ataaagatcg tattgagccg
     3121 ccaaagcacc cgtcagcttg aattgaacgg cttcgatcgc ttcacctgca ccagtcactc
     3181 cggctgcctc gccatttttg gcatagcccg accaaccgct gtcctgacga taagtccgat
     3241 atgaaacgct gcctgcttca gccaaatggg tcaagcgcac ccggacagct tcgagcggca
     3301 aagccacccc accgctgatt gcgccttcct gcacttcggg ctgccaaccg ctgtcctgta
     3361 agtaagtcgt ataaactaat tcagcgttgc tggctgttgc cgagaatagc gggattttct
     3421 gcgcccccga aagggcagcc acacttcttg tttgttgtgt gatatgatcc ttgccggttt
     3481 cggcaagcgt gtcatccaga tttatgtagg ccagctggta aagttccagc aaactttttt
     3541 cgacagctgt accgttctcc aacgaatatg ctggagccgc gatgcggaca ggggctaatc
     3601 cggcaaaaat gaacaacccc aacagaataa acaaaatgtt ccttctcata ataatccctc
     3661 ccggcaggac cgtaagcgac aggaaatgga gcggccgttt gtagccgctc tctttcctcc
     3721 attataccaa acaaagcccc gttaaaaaaa cacaagcgga caaaagacac ggataactgc
     3781 aaaaagttca ggatcgcttc aaaatgaagc gaccttgaac tttttctaag tcattctact
     3841 gttctctttt ccggaaagcc aacaccatcc cgatctggaa gtagctgtaa tcgtccccca
     3901 gcttttcctt gagcggtttc aggtgctgat cgtcggattc ctcgattgct ctggcgatgg
     3961 ccttcgcctg ctcatccgtg acgaaatcct tcaaacgcat ttcgtatgcc gacaactcag
     4021 ccaaatgccg aaaatggttt tcgatggtca tccgtgcaag gcctcggatg gcgatgatct
     4081 cttcgaccgt cttgcattcc tggtacaggc gataagtctc ctcaaaggtg gtaccggcgt
     4141 aatttttctg tgccggtttt gggcgctttt ctgcggtttc cggattgcct gccagctctt
     4201 tctgctccaa ttgtttttgc tgcagaaccg cttgggcatc aggaaactcc ttcagataat
     4261 cggaaatgac gcccatgaac acttcaccgt agcgctccaa cttcgtttcg ccgactcccg
     4321 acaggcgaag gaaggcgtga tcgtcggtcg gaaagttgcg ggccatctca tgcagcgtgc
     4381 tgtccgaaaa gacgatatag gaagggatcc catgctcatt cgccatttcc gtacggacac
     4441 tgcgcagtcg gtcgaacagc tcggtatcgt acaagccgcc tccgctctgt ttggcggtgc
     4501 ccctgccggt ctcgaccgca taggacatcc cgattttttt cccttccttc agtacttcca
     4561 gcgcttttgg agtgacttgg atcaaaggat actgatcgcc cgtcacctgc aggtattcat
     4621 cagcgatcaa taccccgatc atttcgcgga tttccccatc attataggcg ctcatgaggc
     4681 cgtacgtctt cagcttatcc agcccgaaat cggtgatgcc cttctgtttg ctgcccgtca
     4741 ggatgccgat gattttctgg acgccgtaac gtccgcgcac ccgcacaacg caggacagga
     4801 ttttttgggc ttcttcggtg atgtctatct gctccgtgcg gtgtaggcag ttggagcagt
     4861 gttggcaatt cagctcagct gtcgtttcgc cgaaatattc caggatgtac cggcgcaggc
     4921 attggttcgt gtaacagtaa ttgatcatct tgttcaattt attggtcgct tcgctgtcgt
     4981 tcgtcttgct gttcagcaac tgattgatga tgatgtcctg cgtcgaaaac agcaggatga
     5041 cacgcgcttc ctcgccgtca cgccccgccc ttccggcttc ttggtaatag ctttcgatgt
     5101 ttttgggcat gttgtagtgg taaacggtcc gcacattgct tttgtcgatc cccatcccga
     5161 aagcattcgt tgcgaccatg atcggtttgc ggtcgaacag gaaatcatcc tggttccgct
     5221 ggcgctcgtg ttccggcagc ccggcatggt acagcgtcac agcaaagcct ttttgttcca
     5281 attttttctg gaccgattcc acattttttc tggtcgagca atagatgatg ctcgcttcct
     5341 tcgggttgag gtccttcacg agaaacttga acttatcggc gggcttttcc acgctgaagg
     5401 ccaaattgct gcggtcaaat cccgtcacca agcggaacgg ctgttgtagg cgcagctgga
     5461 tcataatatc gtcctggacc ggttcggtgg cagtggcggt gaaggcggca accctcggac
     5521 gtctgccaaa aacatcgtac agtctggcca caccttggta ggaaggccgg aaatcgctgc
     5581 cccactgtga gatacagtgg gcctcatcga ccaccagtaa cggcacattc acaaagcgca
     5641 gagcattcat gaatgactca ttgtctatcc gttccggcgc gatgtagagc agcttgagct
     5701 gtccggagcg catgtcgtcc atcaattgca gaaactcttc tcttgaaagc gtgctgttca
     5761 ggtatgccgc ggcaatcccc atttcccgca ggctgtccac ctgatccttc atcaaggata
     5821 cgagcgggga aacgacgatc gtcatgccgt ccagcagtaa cgccggcaat tgatagcaga
     5881 gcgacttccc tccgctcgtc ggcatgatcc cgagcacgtc tttcccctgc agggtgttcg
     5941 tgatcagttc ctcttggccc tccctgaagc tgtcgtagcc gaaatatttt ttcagagttc
     6001 catacagtaa ttctttatcc accacttgca tccatcctta attgtttatc ttttcttatt
     6061 aaccgaatat ttctgaacgg gcttgccttc actcaaggaa gcaatggcaa accgtcagaa
     6121 atgacttctt catcatatca catctgcggc ctcttttttc gcacaaaaaa tagtacggcg
     6181 ctcccatgag tgaaacctct acttataaaa tatgcagatt atccgcctta tgttttgacg
     6241 tgtggtatat aattgtaagg tgaacacgtc atgacctaca ggagtgagaa atatggaaca
     6301 ggaattatca aatgaactat ttatctgcgg ggggtgcaat gcgaaaatcg gccccggcgt
     6361 gctgggcaat cttctcagcc agtttccgaa acaggaaaac ccgaattttt tggtcggttt
     6421 cgactcgtcc gatgatgcgg cagtgttccg gatagacgcc gagacggcca tgatccagac
     6481 gttggacttc tttcccgcaa tggtcgcgga tgcctcgctt ttcggggaaa tcgctgctgc
     6541 caatgccttg agcgatgtct ttgcgatggg cggcgaagtg ctgacggcta tgaacatcgt
     6601 ctgctggccc gaaacaaaaa gtaccgatac actcgggaaa atattggcgg gcggcttcag
     6661 gaaagtccag gaggcaggcg gcgtgctggt cggcggacat tcgatccacg acccccagcc
     6721 caaatacggt ctatcagtat tgggaagagt ccatcccgat cggatctacc ggaatgatac
     6781 ttgtctgccg ggcgatgtcc tgctgctgac gaagccattg gggaccggca tcatcacgac
//
LOCUS       DAOMUJ010000037         6765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3009_length_6765_cov_2.665127, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000037 DAOMUJ010000000
VERSION     DAOMUJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_6765_cov_2.665127"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2230
                     /gene="secA"
                     /locus_tag="PLT73_01765"
     CDS             <1..2230
                     /gene="secA"
                     /locus_tag="PLT73_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRM19140.1"
                     /translation="LTATMPVYLNALSGEGVHVVTVNEYLASRDAVEMGELYRFLGLT
                     VGLNTNGKSSEEKREAYNCDITYSTNNELGFDYLRDNMVVYKNQMVQRPLNFAVVDEV
                     DSILIDEARTPLIISGQAEKSTALYNRADFFIKGLKENEDYTIDLTSKSIALTDEGIE
                     KAEKTFHLPNLYDVDNTRLVHHLDQALRANYIMLVDIDYVVEDGKVKIVDQFTGRIME
                     GRRYSDGLHQAIEAKENVEIENESKTMATITFQNYFRMYKKLSGMTGTAKTEEEEFRE
                     IYNMNVISIPTNRPIVRHDYPDLLYPSLKSKFRAVVGDIKERHAKGQPILVGTVAVET
                     SELLSHMLRQENIPHQVLNAKNHFREAEIIIGAGQPGAVTIATNMAGRGTDIKLGKGV
                     KELGGLCVIGTERHESRRIDNQLRGRSGRQGDPGETQFYLSLEDDLMRRFGSERIQQV
                     WSKLNLDDEAEDVVIQSKMLSKQVESAQKRVEGNNYDTRKNVLEYDEVMREQREIIYG
                     QRLEVINETESLKYVVTAMIKRAIARVVETHTIGEKATWNLQGIRDFAGAALVHPDSI
                     SLAELEGKSAHEIEEYLVERAMEVYKTKESQLSPEQILEFEKVVILRVVDSKWTDHID
                     NMDELRQGIGLRSYAQNNPLTEYQTEGYDRFQEMIATIDYDVTRIVMKSEIRQNLQRQ
                     SVGQGSNIMPTRETVASQTEQQEQARRAQIAAMRMQKLMEIQKAKLAAEAAEKQESQT
                     EE"
     gene            2390..3506
                     /gene="prfB"
                     /locus_tag="PLT73_01770"
     CDS             join(2390..2461,2463..3506)
                     /gene="prfB"
                     /locus_tag="PLT73_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119093859.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRM19141.1"
                     /translation="MELSEIKAILESSETKIASFRRSLDLDGLETDIAEFDDRMLDPE
                     FWNNAEAAQEVINQSNQLKAVYNTFKSLEAQYEDLEVLYEMVKEDEDPEYLEELNEKT
                     TEFTEALEKYELTMLLNGPHDKSNAILEIHPGAGGTESQDWGSMLFRMYTRWADKKGF
                     DVETLDYQSGDEAGIKSVTILIKGMNAYGYLRSERGVHRLVRISPFDSAGRRHTSFCS
                     IDVMPEIAADDAEIEVNPDDIRVDTYRASGAGGQHINKTSSAIRITHIPTGIVVASQA
                     QRSQFQNRDTAMGMLKAKLYQLQEEEREKELAAIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRSSYETGNVQAVMDGDLDAFIDAYLKMTMDQEEL"
     gene            3689..4375
                     /gene="ftsE"
                     /locus_tag="PLT73_01775"
     CDS             3689..4375
                     /gene="ftsE"
                     /locus_tag="PLT73_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRM19142.1"
                     /translation="MIEMLNVSKKYNNGITAVNNLSVKIEQGEFVYIVGPSGAGKSTF
                     IKMMYREVKPTSGTVQVGKYNLTTMKEKEVPLLRRFVGVVFQDFKLLPKLTVYENIAY
                     AMEVVEKSPKVIQKRVLEVLDLVGLKHKVRMFPNELSGGEQQRIAIARAIANMPGVLI
                     ADEPTGNLDPDTSMEIMRILEEINNQGTTILMATHNSKIVNDIKHRVLAIENGRIVRD
                     QQEGEYGYEI"
     gene            4365..5252
                     /gene="ftsX"
                     /locus_tag="PLT73_01780"
     CDS             4365..5252
                     /gene="ftsX"
                     /locus_tag="PLT73_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRM19143.1"
                     /translation="MKFRTFMRHIRDAFKSLFRNGWMSIASISAVALTLLLVGSFTGI
                     LLNVNKLASDVENDVSVKVYIDLAADAAAQEALKTELENIANVESITFSSKDEELDKI
                     IGSYGDEFDLFGGDANPLYDAYVLNTTAPEQTKAVAEAASAMQYVTQVEYGGATADKL
                     FETIATIRTIGLVIIIGLLLVAVFLISNTIRITIFSRSTEIEIMRLVGAKNSFIRWPF
                     LIEGAIIGLIGALIPMTFLVFLYRFIFNVSGDYLAGTNFGLLTPNPFLLYISLLMVGI
                     GIFIGAFGSVFSMRRFLKV"
     gene            5607..6338
                     /locus_tag="PLT73_01785"
     CDS             5607..6338
                     /locus_tag="PLT73_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM19144.1"
                     /translation="MKKVLLVDDEPSIVTLLAFNLEKDGYEVTTATDGAEGYRLAISN
                     PFDFIILDLMLPSMDGMDICKRLRQEKFDTPIMILTAKDDELEKIIGLELGADDYMTK
                     PFSPREVLARMKAILRRTNKAVPAEPVATAQPEPTEDETEKIEVGEITIFPQLYEVHV
                     AGELIEVTPKEFELLLYMAKRANRILSREQLLNAIWNFDYAGETRIVDVHISHLREKI
                     EKDTKNPQYIRTVRGFGYKFEVAKT"
     gene            6335..>6765
                     /locus_tag="PLT73_01790"
     CDS             6335..>6765
                     /locus_tag="PLT73_01790"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRM19145.1"
                     /translation="MKQLQLRMLATFVLIFALFSVAFGIFLETVLGNYTRSLHSELLV
                     EQTQIVGTLLQNSAVGSDWTFVEKELDKIDSFTEDRITLVASDGVVIYDSQLTAVNLE
                     NHLRREEIQEVLSGEPYGTGQRKSESTNQELYYVAVPIFDES"
BASE COUNT         2051 a         1461 c         1703 g         1550 t
ORIGIN      
        1 cttgaccgca acgatgccgg tttacctgaa cgccttatct ggcgaaggcg tgcacgttgt
       61 cacagtcaat gaatatctgg caagccgtga tgcggtcgag atgggcgaat tgtaccgctt
      121 tctggggttg actgttggtt tgaacacaaa cggtaagtca tcggaagaaa aaagagaagc
      181 ctataattgc gacatcacct acagcaccaa taatgaattg ggcttcgatt atttgaggga
      241 caatatggtc gtctataaaa atcaaatggt gcagcgcccg ttgaactttg cggtcgtgga
      301 tgaagtcgat tcgatcttga tcgatgaagc ccgcacacca ttgatcattt ccggacaagc
      361 cgaaaaatct acagcactct ataaccgagc cgattttttc atcaaaggat tgaaagagaa
      421 tgaggattac acaatcgatt tgacatccaa atcgatcgcg ttgacggatg aaggtatcga
      481 gaaagctgaa aaaacattcc atctgccgaa cttgtacgat gtggataaca cgcgtctggt
      541 gcaccatttg gatcaagcct tgcgtgcaaa ctatatcatg cttgtggaca tcgattatgt
      601 ggtggaagac ggaaaagtaa aaatcgttga ccaattcact ggccggatca tggaaggccg
      661 ccgttactcg gacgggcttc accaagccat cgaagccaag gaaaacgtcg aaatcgaaaa
      721 tgaatcgaaa acgatggcca ccatcacttt ccagaactac ttccgtatgt acaaaaaact
      781 gtccggtatg accggtacag caaaaacgga agaagaagag ttccgggaaa tttacaacat
      841 gaacgtcatt tcgattccga ctaacagacc gatcgtccgt cacgactatc cggatttgtt
      901 gtatccttct ttgaaaagca aattcagagc cgttgtcggc gacattaaag aacgtcacgc
      961 gaaagggcag ccgatccttg tcggtaccgt agccgtggaa acatccgaac tgctttcgca
     1021 catgttgcgt caagaaaaca ttccgcacca agttttgaat gcaaaaaacc atttccggga
     1081 agcggaaatc atcatcggag ccggtcaacc cggagccgtc acaatcgcca ccaacatggc
     1141 tggtcgtggt accgacatca agctgggtaa aggcgtcaag gaattaggcg gtctctgcgt
     1201 catcggtacc gaacgccatg aatcacgccg gatcgacaac caattgcgtg gccgttccgg
     1261 ccgtcaaggg gatcccggcg aaacgcaatt ttacctgtca ctggaagacg acttgatgag
     1321 acgcttcgga tcggaacgca tccagcaagt ctggagcaaa ttgaatttgg atgatgaagc
     1381 ggaagatgtg gtcatccaaa gcaaaatgct ttccaaacaa gtggaatccg cccaaaaacg
     1441 agtggaaggc aataactatg atacccgtaa aaacgtactg gaatacgatg aagtcatgcg
     1501 tgaacagcgt gagatcattt atgggcaacg tttggaagtc atcaatgaaa ccgaatcgtt
     1561 gaagtatgtc gtgacagcga tgatcaagcg tgcgattgcg cgtgtggtcg aaacgcatac
     1621 catcggtgaa aaggcgactt ggaatctgca aggcatccgt gattttgccg gagctgccct
     1681 tgtgcatccc gacagcatca gtttagccga actggaagga aaatctgcgc acgaaatcga
     1741 agaatatttg gtggaacgcg caatggaagt ttacaagaca aaagagtcac aattgagtcc
     1801 agaacaaatt ttggaatttg aaaaagttgt tatcctgcgg gttgtcgaca gcaaatggac
     1861 cgatcacatc gacaatatgg atgagcttcg ccaaggaatc ggtttgcgtt catatgcaca
     1921 gaacaatccg ttgaccgagt accaaaccga aggctatgat cgtttccagg aaatgattgc
     1981 gaccatcgac tatgacgtta cccggattgt gatgaaatca gaaattcgtc aaaatctgca
     2041 acgccaatcc gtaggccaag gctcgaacat catgccgacg cgtgagacag tcgcttccca
     2101 aacggagcaa caggaacaag cacgcagagc acagatcgcg gcaatgagga tgcaaaaact
     2161 gatggaaatc caaaaagcaa aactggcggc tgaagcagcc gaaaaacaag aatcacaaac
     2221 tgaagaataa caggcagcgc atccgcaaga ggcatttcaa gcaataaaac caagtcaaat
     2281 acaacaaact aatcggatac aacgccaatc ggggagggtc gggataaaaa acagtcgctt
     2341 gtttttatcc cgacccatcc ttcttttgtc gaatctagga ggaaatcaaa tggaattaag
     2401 tgaaatcaaa gcaatattgg aaagttcaga aacaaaaatt gcaagcttca ggaggtctct
     2461 ttgacttaga tggcctcgaa acggatatcg cagaattcga cgatcgcatg ttggatccgg
     2521 aattctggaa caatgccgaa gcggcgcagg aagtcatcaa ccaatcaaac caattaaaag
     2581 cggtctacaa tacgttcaag agtctggaag cgcaatatga agacctcgaa gtcttatatg
     2641 aaatggtaaa agaggatgaa gatccggaat acctggaaga attgaacgaa aaaacgacag
     2701 aatttacaga agcacttgaa aaatatgaat tgacgatgct gttgaacgga ccgcacgaca
     2761 aatcgaacgc tattttggaa atccatccag gtgccggcgg aacggaatcc caggactggg
     2821 gcagcatgct tttccgcatg tatacgcgtt gggccgacaa aaaaggcttt gacgtggaga
     2881 cgttggatta tcaaagtggg gacgaggccg gtatcaaaag cgtaacgatc ctgatcaaag
     2941 ggatgaacgc ctacggctac ctgcgttcgg aaaggggcgt ccaccgtttg gtccgcatct
     3001 cgccgttcga ctctgccgga agaaggcaca cctcgttttg ttcgatcgat gtgatgccgg
     3061 agatagccgc cgacgatgca gagatagaag tgaatccgga tgatatccgg gtggatactt
     3121 accgcgccag cggagccggt ggacaacaca tcaataaaac atcatcagcc atccggatca
     3181 cgcatattcc gaccggtatt gttgtggcaa gccaggcgca acgttcgcaa ttccagaaca
     3241 gggataccgc tatgggaatg ctgaaggcga aactgtatca actgcaggag gaagagcgcg
     3301 aaaaagaact ggccgccatc cgcggcgagc aaaaagagat cggttggggt tcacagatcc
     3361 gttcatacgt gttccatccg tactccatgg tcaaggatca ccgaagcagc tacgaaacgg
     3421 gtaacgtaca ggccgtcatg gacggggatc tcgacgcctt tatcgatgct tatctgaaaa
     3481 tgacgatgga tcaggaagaa ctttaaaagc agcagcccaa ataaatagga tcagtcacga
     3541 gcgagaatac tgagaaacta ttgacacata cttgtaaaga aactaaaaca gacacagttg
     3601 tgcaacgtgc tataataggg aagcattctt gcagcctctg aaggtttcag agcggaagca
     3661 aggaaaaaaa gaaaagacag gtgataaaat gattgaaatg ctgaatgtct ccaagaagta
     3721 caacaacggc attacggcgg tcaataatct gagcgtgaag atcgagcaag gggaatttgt
     3781 ttatattgta ggccccagtg gagcgggcaa atcaactttc atcaaaatga tgtatcgcga
     3841 agtaaaacca acctcaggaa ctgttcaagt aggcaaatat aatttaacga ccatgaaaga
     3901 aaaagaagtc ccattattgc gccgttttgt gggtgtggtc ttccaggatt tcaaattatt
     3961 acctaaactg accgtatacg aaaacatcgc ctacgcgatg gaagtagtcg aaaaaagtcc
     4021 gaaagtcatc cagaagcgcg tattggaagt gttggatttg gtcggactga agcataaagt
     4081 gcggatgttc ccgaatgaac tttccggtgg ggaacagcaa cggatcgcga ttgcacgtgc
     4141 gattgcgaat atgccaggcg tcctgattgc ggatgagccg acaggtaatt tggatccgga
     4201 tacgtcgatg gaaatcatgc ggattctgga agaaatcaac aaccaaggaa caacaatcct
     4261 gatggccacc cataacagca aaatcgttaa tgacatcaaa caccgtgtat tagcaataga
     4321 gaatggccgt attgtccgag atcaacaaga aggagaatat ggatatgaaa tttagaacat
     4381 ttatgagaca cataagggac gcattcaaaa gtctgttccg gaatggctgg atgtcgatag
     4441 cttcaatcag tgcagtggca ctgactttgt tgcttgtggg gtcttttacc gggattcttt
     4501 tgaacgtgaa caagttggct tccgatgtcg aaaatgatgt gagcgtaaaa gtttacatcg
     4561 atctggctgc tgatgcagcc gcccaggaag ccttaaagac ggaactggag aacatcgcca
     4621 atgtcgaatc catcactttc tcaagtaagg acgaggaatt ggataagatc atcggcagct
     4681 acggtgatga attcgatctt ttcggcggag atgccaatcc gttgtatgat gcttatgtct
     4741 tgaatacaac tgcaccagag caaacgaaag cggtagctga agcagcatcc gccatgcagt
     4801 atgtcactca agtcgaatac ggtggcgcca cagcggataa attatttgag acgattgcga
     4861 cgatccgcac gataggactc gtcatcatca tcggcttact gttggttgcc gtattcctga
     4921 tttccaatac aatccggatt accatcttct cccgcagcac ggagatcgaa atcatgcgtt
     4981 tggtgggcgc aaaaaacagc ttcatcagat ggccattcct gatcgaagga gccatcatcg
     5041 gattgatcgg ggcattgatt cccatgactt tcctagtgtt cctttaccgc ttcatcttta
     5101 atgtgagcgg ggactacttg gccggcacaa actttggtct tttgactccg aatccgttcc
     5161 tcctctacat cagtcttctg atggtgggta tcggtatctt catcggggca ttcggttccg
     5221 ttttctcgat gcgccgcttc ctgaaagttt aacaaaaaat ttttgaccca taaggttcta
     5281 gtcatacaaa acgtatgggt ggaacctttt ttgtcgttcg cataagcagg ctcatcctga
     5341 aaaccgataa actgaaacta ttattttgta tgcatttatt tctcttttac cttgtggaat
     5401 ctcctgtaca gcgcactttt tagagtcttt tcggttgatg gataaaaatc ggccgggaaa
     5461 atccgaattg ccgtttttca acatttttag tatgctgaaa aggaaacgaa ccgagcagga
     5521 aaccgaggct ctcaaaaata cggccattcg taaaatacat ggggggcata cgccatcccg
     5581 gaccctaatc ggtcagagga ggacacatga aaaaggtatt gctagtggat gatgaaccat
     5641 cgatcgttac attattggct ttcaacttag aaaaagacgg ctacgaggtg acaactgcca
     5701 cagacggagc ggaaggttac cgtttggcta tctcgaatcc ttttgacttt atcattctgg
     5761 atctgatgct accgtcaatg gacggaatgg atatctgtaa acgcctgagg caggaaaaat
     5821 ttgatacacc gatcatgatt ttgacggcga aggatgatga gctggaaaag attatcggac
     5881 ttgagttggg cgcggatgat tatatgacaa agccattcag cccgcgtgaa gtattggcca
     5941 ggatgaaagc tatcctaaga aggacgaaca aagctgttcc ggctgaaccg gttgcgactg
     6001 cccaaccgga accgacggag gacgaaacag aaaaaatcga agtcggcgag atcaccattt
     6061 ttccgcaact gtatgaggtt catgtagcgg gagaactgat tgaagtgacg ccgaaagaat
     6121 ttgaattgct gctttacatg gcgaagcgcg ccaatcgtat cctgagcagg gaacagttgt
     6181 tgaatgccat ctggaatttt gattacgccg gagaaacgcg catcgtcgat gtacacatca
     6241 gccatctccg cgaaaaaata gaaaaagaca ctaaaaatcc gcaatacatc cgtacggtcc
     6301 ggggcttcgg gtataaattt gaggtagcga agacatgaag caactgcaat tgcggatgct
     6361 tgccactttt gtgttgatct ttgcgctgtt cagtgtggct ttcgggatat tcctggagac
     6421 tgtgttaggg aactacacgc ggagtctgca cagtgaactg ttggttgaac agacgcaaat
     6481 agtgggtaca ctgctccaaa acagtgctgt cggcagtgac tggacctttg ttgaaaaaga
     6541 attggataaa attgactctt tcacggaaga tagaatcact ttagtcgcca gcgatggggt
     6601 tgtgatttat gattctcagc tcactgcggt caatctggaa aatcatctgc ggcgggaaga
     6661 aattcaggaa gttctgtccg gagagcctta cggaacagga caaaggaaga gcgaaagcac
     6721 aaatcaggag ctttattatg tagctgtgcc gatttttgac gaatc
//
LOCUS       DAOMUJ010000038         6697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3056_length_6697_cov_2.338302, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000038 DAOMUJ010000000
VERSION     DAOMUJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_6697_cov_2.338302"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLT73_01795"
     CDS             complement(<1..903)
                     /locus_tag="PLT73_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM19146.1"
                     /translation="MKYLKPFAGTILVSLLILFGQAIGELSLPNLMSDIVNTGIQNGG
                     VEQEAPEALPAEGFALMTLFMDEEDKETFQESYELIEGGSAEAEAYNAEFPESQETDF
                     YVLDETDPEQLISLESIFSKASYSFVTFMQEMGGQQGGTAAVDAESGFENVDLDQLYA
                     LVPQLQQLPQEAFTASMDVADAAEPMLYSQIGILFTKLFYEDLGADMDALQNQYIMTK
                     GAQMLAIAVAVSVASVAVAYFSSRIAAKVSGRMRRDVFAKVESFSNTEFDNFSTASLI
                     TRTTNDVQQIQQLITIGIRMLCYAP"
     gene            complement(941..1426)
                     /locus_tag="PLT73_01800"
     CDS             complement(941..1426)
                     /locus_tag="PLT73_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM19147.1"
                     /translation="MISREMMGAYIPMLHNQFKEQMNLLVGKIDLTTSQIHVLFYLKS
                     RGDEEVIQKDIEETFNLTNPTVTGIIKRLEAKGFVTRTVSSKDARSKSIHLTEKSIAA
                     SEEMKRAMHEANKKVFEGFTEEELDVLEECFIRMLHNLEGGCSHQHGHGHGHVHKKEN
                     N"
     gene            1580..1957
                     /locus_tag="PLT73_01805"
     CDS             1580..1957
                     /locus_tag="PLT73_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRM19148.1"
                     /translation="MEETISVRDRLIALCQEHAHVIEDYPFGDSEWTVMRHEGNRKTF
                     AYIYERNGEVWLNVKNTPERNDFLRETIPEIMPAYHMNKTHWITLRIVSEELFPVADR
                     IITESYRLTQTKPRKKTGVQDLH"
     gene            complement(2264..>2430)
                     /locus_tag="PLT73_01810"
     CDS             complement(2264..>2430)
                     /locus_tag="PLT73_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HRM19149.1"
                     /translation="KFHQVKKMFLSVGKKVTYLKRLTMGPIELDESIPPGSYRPLNEA
                     ELEQLKPYFR"
     assembly_gap    2431..2454
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2455..2965)
                     /locus_tag="PLT73_01815"
     CDS             complement(<2455..2965)
                     /locus_tag="PLT73_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM19150.1"
                     /translation="MRLDKLLSECGYGSRGQVKKLIRSKQVSIDGEIALAENRNVDPQ
                     LQTVIVSGKTLVHRTNVYYMLHKPAGVVTAVSDEGNRTVIDLIDQSDRRPGLYPVGRL
                     DKDTEGLLLITDNGQLGYQLLLPHKKVTKRYEVVVNERVTVADQEAFAEGIVFHGGTT
                     CKPARLTILS"
     gene            3195..3488
                     /locus_tag="PLT73_01820"
     CDS             3195..3488
                     /locus_tag="PLT73_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRM19151.1"
                     /translation="MFIQARKLSIARGFSEAAVNKFKTSTLLPQQPGFDSIETYVRSV
                     NKESEEVLVLIKWGTKKDWQNWEKSDVHKAGHRIKKERPEYILGMEMSYYESF"
     assembly_gap    3493..3506
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3639..4181)
                     /locus_tag="PLT73_01825"
     CDS             complement(3639..4181)
                     /locus_tag="PLT73_01825"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRM19152.1"
                     /translation="MKKWRNGIVGGTLAVLLFSGTGVMASEDEELYGAAAVSEGETYT
                     IEEMLVYAIQDEYMAEASYLAIMDAYGTIKPFTSISKAEGTHISLLLPLFETYGFEVP
                     ENEAEARIELPASLAESFEKGVAGEIENISVYGQFLGAEDLPDDVRSVFERLMTASEK
                     HLAAFERGVDGNPDGAGRRK"
     gene            complement(4321..4656)
                     /locus_tag="PLT73_01830"
     CDS             complement(4321..4656)
                     /locus_tag="PLT73_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018369457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HRM19153.1"
                     /translation="MNLHAVLTEVIPTLASLIEGIGVLIILYGCVKSLYLFVIDRLNL
                     SNNQPKLELAKALAFSLEFKLAAEILKTVIIQTIDEFIVLSAIVILRVVLTVVIHWEI
                     KSTNSEELD"
     gene            complement(4714..5082)
                     /locus_tag="PLT73_01835"
     CDS             complement(4714..5082)
                     /locus_tag="PLT73_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRM19154.1"
                     /translation="MGETTIKNETIDAYISSYDEVVQPILKELRRVIKEEAPDATEKI
                     SYQMPTFYLNGNLVHFAVQKNHIGFYPAPSGVAAFKEELTEYKTSKGAIQFPLTKPIP
                     YELVRRIVRFRVEEAREKKK"
     gene            5320..6384
                     /gene="rlmN"
                     /locus_tag="PLT73_01840"
     CDS             5320..6384
                     /gene="rlmN"
                     /locus_tag="PLT73_01840"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRM19155.1"
                     /translation="MNTPSIYGLTLDQLEAWLLEQGQKKFRAQQIWDWLYIKRVLDFS
                     EMTNLSKDLVALLSDTFLLQPLNQVVVQESADGTTKYLFQLEDKLMIETVLMRQEYGL
                     SVCVTTQVGCDIGCTFCASGLKRKQRDLTAGEIVAQIMQIQHYLDQKGNGERVSHIVV
                     MGIGEPFDNYDNVIAFLNIVNHAKGLAIGARHITVSTSGLAPKIREFAENGLQVNLAL
                     SLHAPNDEIRSRMMRINRKHPLKEVMAAIDEYLEKTNRRITFEYIMISGVNDRPEQAH
                     ELVALLKDKRHLSYVNLIPYNPVAEHIKYERSTKKDILAFYDILKKNNINCVIRKEHG
                     TDIDAACGQLRSKHLEKATK"
     gene            complement(6498..>6697)
                     /locus_tag="PLT73_01845"
     CDS             complement(6498..>6697)
                     /locus_tag="PLT73_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19156.1"
                     /translation="IAVLFAIVYYHSRVNAYSCPQCRRKFKISFLKDLLSLNGGKQGK
                     RLTCPHCGFKGWVQETAPDEE"
BASE COUNT         1611 a         1709 c         1506 g         1833 t
ORIGIN      
        1 cggtgcatag cacagcatgc ggatacctat agtgatcagc tgctggattt gctgcacgtc
       61 attggttgtg cgcgtaatca aggatgctgt tgaaaaatta tcgaactcag tgttggaaaa
      121 actttcgact ttggcgaaga catcccttct catccgaccg gacactttcg cggcgatacg
      181 cgaagagaaa tacgcgaccg ctacggaagc aacagacacc gcaactgcga tggcgagcat
      241 ctgtgcgcct ttcgtcatga tgtattggtt ttggagcgca tccatatccg ctcctagatc
      301 ttcatagaat agtttcgtga acaggatccc gatttggctg tagagcattg gctcggcagc
      361 atcggcaaca tccatcgatg cagtgaacgc ttcctgtggc agctgttgca gttgcggcac
      421 caacgcgtac agttgatcca aatcgacatt ctcgaagccg ctctcggcat cgactgccgc
      481 ggtaccgcct tgctgtccgc ccatttcctg cataaacgtc acgaagctgt agcttgcctt
      541 gctgaagatg ctctccaacg agatcagttg ttccggatcg gtttcatcca gcacgtagaa
      601 atccgtttcc tggctttccg gaaattctgc gttgtaggcc tcggcttccg ccgatccacc
      661 ctcgatcagc tcatagcttt cctggaacgt ttccttatcc tcttcatcca tgaacaatgt
      721 cataagcgcg aaaccttccg ccggcagtgc ttcaggcgct tcctgttcga cgcctccgtt
      781 ctggatgccc gtgttgacga tgtcactcat cagattcggc aggctcaatt cgccgatcgc
      841 ctgaccgaac aaaatcagca agctgaccag tatcgtaccg gcaaaaggtt ttaagtactt
      901 cattatcact ttcatatgct ttccctattc tttcctaaat ttagttgttt tcttttttat
      961 gcacatgccc gtgtccatgt ccatgttggt ggctgcagcc gccttccagg ttgtgcagca
     1021 tccttatgaa gcattcttcc aacacgtcca gctcctcttc agtaaagcct tcgaaaactt
     1081 ttttattggc ttcatgcata gcccgcttca tctcttctga ggcggcgatg cttttttcgg
     1141 tcagatgaat gctcttggag cgggcatcct tcgaactgac cgtgcgtgtg acgaaaccct
     1201 tcgcttccag ccgtttgatg attccggtca cggtcggatt agtaagattg aaagtctcct
     1261 cgatgtcttt ttgaatcact tcctcgtcac cacggctctt cagatagaaa agcacatgaa
     1321 tctgggaagt ggtcagatcg attttaccca ccaggaggtt catctgttcc ttgaattggt
     1381 tatgaagcat cgggatatag gcccccatca tctcgcgtga tatcaaaatg tctcctcctt
     1441 tccgaacagt tatacatagc acgctatcta ttatcgcatt atccatccat atgtcaatag
     1501 attcgatccg atgcgagcca ttttcggaaa aattgaatat aatggagata gcatcaaatg
     1561 aattggaaag gagatcagca tggaagaaac aatttctgtc cgggatcggc ttattgccct
     1621 ttgccaagaa cacgcgcatg tcatcgaaga ttatccgttc ggcgactcag aatggaccgt
     1681 catgcgccac gaaggcaacc ggaaaacttt cgcttacatc tatgaacgga acggtgaagt
     1741 ttggctgaac gtaaagaata ctcccgagcg gaacgacttt ctgcgcgaga cgattccgga
     1801 aatcatgccc gcctatcata tgaacaagac ccattggatc accttgcgga tcgtctccga
     1861 agaactcttc ccggtcgctg accggatcat cacggaaagc taccggttga cgcagaccaa
     1921 accgaggaaa aaaacaggtg tacaggatct gcattgaaca ttagtcagct tcttctttct
     1981 tgtttgctga tatgattgtc tgatcttggg ttcgcgttta tgattgtaag cgagattccc
     2041 cgccaaaatg gttttgacgg ggaatctcgt gttgtccgcg gacgtttttc cccgccaagg
     2101 ttgttttggc gggttttctg actgtagttt gagttgtttt cccccgcctg accgcttttg
     2161 gcgggttatt actgagcagt cattcatttc ccaaatagaa ggggctgtat aaaaccagaa
     2221 tagtgctttc tggttttata cagccccttg atttgccttc tttctagcgg aaatacggct
     2281 tcagttgttc caattccgct tcgttgagcg gccggtagct gcccggcgga atcgattcat
     2341 ccaattcgat cggtcccatc gtcaggcgtt tcaggtaagt gacctttttg ccgaccgaaa
     2401 gaaacatctt cttcacttgg tggaatttcc nnnnnnnnnn nnnnnnnnnn nnnnggctga
     2461 ggatggtcaa ccgggcaggt ttgcaggtcg tccctccgtg gaagacgatt ccttcagcga
     2521 aggcttcctg atcggcgacg gtcacgcgct cgttcacgac gacctcgtag cgtttcgtca
     2581 cttttttatg cggcagcaac agctgatagc ccagctgccc attgtccgtg atcagcagta
     2641 gcccttccgt gtccttgtcc agccgtccca caggatagag acccggtctg cggtcggact
     2701 ggtcgatcag atcgatgacg gtgcggttgc cttcatcgga taccgccgtc acaacgccag
     2761 ccggtttgtg cagcatatag tagacattgg tccggtgcac cagcgttttc cccgaaacaa
     2821 tgaccgtctg tagttgcgga tcaacattgc ggttttccgc cagcgcgatt tccccgtcga
     2881 tgctgacttg tttgcttctg atcaattttt tcacttgtcc tctcgaaccg tagccgcatt
     2941 cgctgagcag tttgtccaat cgcatccagc ctcttccttt ctgtccttat tgcagtcctt
     3001 ttattctaag cggaaaagat acttgacgca agttctcgca aaaaaccaga acgtgaatcc
     3061 attattccca tatgctcaga tttctttcca cattattttc taatctttcc cttacacttt
     3121 ccagccagtt ctgctatact atccactata taaaaataag ctgtatttat tataaatgaa
     3181 agaaggtagc gcccatgttc atccaagcca gaaaattatc gattgcccgc ggtttctcgg
     3241 aggccgctgt aaataaattc aagaccagta ccttactgcc tcaacaaccc ggtttcgaca
     3301 gcattgagac ctatgtccga tccgtcaaca aagagagcga agaagtgctc gtcctgatca
     3361 aatggggcac aaaaaaagat tggcagaact gggaaaagag cgatgtccat aaagccggtc
     3421 atcgcattaa gaaagagcgt cctgagtata tcttgggtat ggaaatgagc tactacgaaa
     3481 gtttttgaca atnnnnnnnn nnnnnngatt gcttgtgggt tgcttggagg ttgtccgatt
     3541 cttcgttgtt atcggacaac cagcagatca aacactcata cgtaacaagc cccgcgcaag
     3601 tttattctgc gcggggcttg ttacgtatgg aattcacatc attttctcct gccagctccg
     3661 tcgggatttc catcaacgcc gcgttcaaag gctgccaaat gcttttcgga tgcggtcatc
     3721 aagcgttcaa agacgcttct gacatcatcc ggcaaatctt cggccccaag aaattgccca
     3781 taaacagaga tgttttcgat ttcaccggcc acgccttttt caaagctttc cgccaaagac
     3841 gcaggcaact caattcgagc ttccgcttca ttttccggaa cttcaaatcc ataagtttcg
     3901 aacagtggta acaataagga aatgtgtgtg ccttccgctt tggagatgct cgtgaatggc
     3961 ttgattgttc cgtatgcatc catgattgca agataggacg cttcagccat gtattcatcc
     4021 tggatggcgt aaaccaacat ttcttctatt gtatacgttt cgccttcgct caccgctgct
     4081 gccccatata actcttcgtc ctcggatgcc atgacgccgg tgccgctgaa caataagact
     4141 gccaaagtcc cgcctacaat tccgttccgc cattttttca ttttgatccg ctcccttttt
     4201 tgtatgtttc atgatcctct tttctacagg ttcagtatag aaaagaagta tgtcgaaagc
     4261 atggcaaaag cggggcgaaa agtgaacgtt gtcattttcc cgagaagatg gaccaagaaa
     4321 tcaatccaac tcttctgaat tggtgctctt gatttcccaa tggatgacga ccgtcagcac
     4381 gacccgcagg atgacgatgg ctgagagcac gatgaattcg tcgattgtct ggatgatgac
     4441 cgtcttcagt atttccgcgg ccagtttgaa ctccagactg aacgccagcg ctttggccaa
     4501 ctcgagtttc ggttggttgt tgctcagatt aagcctgtca attacgaaca gatacaacga
     4561 cttgacgcat ccatagagga tgatcaggac accgatgcct tcgatcagac tcgccaatgt
     4621 cgggatgact tcggttaaga cagcatgcaa attcatgaac atccctcctt tttccatatt
     4681 caaatttggt aacaaatccg ttctttattt ttcttatttc tttttctccc ttgcttcttc
     4741 gacccggaag cggacgatcc ggcgcacaag ctcgtatggg ataggtttcg tcagcgggaa
     4801 ttggatggcg cctttcgagg tcttgtattc cgtcaattct tctttgaaag ccgcaacgcc
     4861 gcttggtgcc ggatagaaac cgatatgatt tttctgcaca gcaaaatgca ccaggttccc
     4921 gttcaggtag aaggttggca tctgatagct gattttttct gtcgcatcgg gtgcttcttc
     4981 ctttatcacc cgacggagct ccttcaggat tggctgaaca acctcatcat agctgctgat
     5041 gtatgcatcg attgtttcat tttttatagt tgtttctccc attttaaatc acctcggtag
     5101 tctggatttt ccgttgttta tgtccctgca aatagtatat catacactga tgaaagtgtt
     5161 tgcaggctaa atggactgac gatcactttc atcgggaatt ccgggcattt gaagtgcttt
     5221 ttctctccct ttcacttctt ttacggaagt catttctttt tgaaaaaaat atgttatgat
     5281 atggattgaa aatttgtacg gtaaagaaag gaaagtaaaa tgaatacacc ttctatttat
     5341 ggattgactt tggatcagct ggaagcttgg ctgttggaac aaggccagaa aaaattccgc
     5401 gcccaacaga tctgggattg gctatatatc aaacgcgtcc tggacttttc ggaaatgaca
     5461 aacttgtcaa aagatttggt tgcccttctc tccgacacct tcttgctgca gcccctgaat
     5521 caagtggtcg tccaagaatc agcggacggc acaacgaaat atcttttcca actggaagat
     5581 aaactgatga tcgaaaccgt tctgatgcgt caggaatacg gcctgtccgt ctgcgttacg
     5641 acccaagtcg gctgcgatat cggctgcacg ttctgcgcaa gcggcctgaa acgcaaacaa
     5701 cgcgatctga ctgccggcga aatcgttgca cagatcatgc agatccagca ctacttggat
     5761 caaaaaggca atggcgaacg tgtcagccat atcgtcgtga tgggcatcgg cgaaccgttc
     5821 gacaactacg acaatgtcat cgccttcctg aacatcgtca accacgccaa aggtttggca
     5881 atcggcgctc gccacatcac cgtttccacc agcggattgg ctccgaaaat tcgcgaattc
     5941 gcagaaaacg gactgcaagt caacttggct ttgtccctac acgcgccgaa cgacgaaatc
     6001 cgcagccgca tgatgcgcat caaccgtaaa catcctctga aggaagtcat ggctgccatc
     6061 gacgaatatc tggaaaagac caaccgccgc atcactttcg aatacatcat gatttctggt
     6121 gtgaacgaca gaccggagca ggcgcacgaa ttggttgcct tgttgaagga caagcgtcac
     6181 ctttcctacg tgaacctgat cccttacaat ccggttgccg aacacatcaa atacgaacgc
     6241 agcaccaaaa aggatatttt ggcattctat gatatcctca aaaagaacaa catcaactgc
     6301 gtcatccgca aagagcacgg caccgacatc gatgctgcct gcggccaact acgcagcaag
     6361 catctggaaa aagcaaccaa ataattgacc tcagcagagg ccgtctcaaa ataaaatgag
     6421 ccggcctctt tttttttgct cgtttctgat tttattggtg ctgttgacct tagcttggtg
     6481 ggtggtcggg ctgattttta ttcctcgtca ggcgcggtct cctgcaccca acctttgaat
     6541 ccgcaatgcg ggcaagtcaa ccgtttgcct tgcttgcctc cgttcaaaga cagcaaatcc
     6601 ttcaggaagc tgattttgaa tttccggcgg cactgagggc agctgtacgc attcacgcgc
     6661 gaatggtagt agacaatcgc aaacagaact gcgattc
//
LOCUS       DAOMUJ010000039         6650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3089_length_6650_cov_2.631084, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000039 DAOMUJ010000000
VERSION     DAOMUJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_6650_cov_2.631084"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLT73_01850"
     CDS             complement(<1..1177)
                     /locus_tag="PLT73_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19157.1"
                     /translation="MNKKVWWKGFFWSINLLFLVILLGFSGVASATTMNAENLDTLAA
                     GETIEGPGFFAGNVVKVDGNVEGTTFVSGQDVRINGNINGDLFVAAQSITINGTVIGN
                     IYGAGQALTFDGQSESDVFFAGQNIAIGQDASVGRDIFVAGATILQEGVIQRNLFGGG
                     AEIVLSGQIGGDVNLQTDSLKLQDGANIAGNLNYKGGNEADIGTGATIAGDTDWEFAE
                     TMDRGTTVMRETRRPFMTVVWFLWSLASALLIWFLIRIWRPAFWQNTARPLTERPLKT
                     LGVGLLTLLVVPPLIILAMVTVIGIPIAIILGTVYPIALYLSRIIVAVFIGQWLAKRF
                     KWPELHKGVWLVLLGLIILGALRMPPYIGFLSSLLIVIAGLGSLVMAHHRVAPETASV
                     "
     gene            complement(1219..2103)
                     /locus_tag="PLT73_01855"
     CDS             complement(1219..2103)
                     /locus_tag="PLT73_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19158.1"
                     /translation="MNKIVPHFWFDTQATEAAEFYTSLFDNSKITNISQIQNPPPYGT
                     ADIVSMTLSGQDFMAIAAGPYFRFNPSISIRIDCASMKEVDFLWEKLSDGGTVLMPLD
                     AYPFSKRYGWTEDRYGLSWQVMHVGEREIEQKLTPTLMFTKKQCGKAEEAILFYEAVF
                     SDTSIDAMDYYGVGEEPDAPGTVKFASFKLEDQAFAAMDSAQAHDFEFNEAFSFIVNC
                     ENQEEIDYYWEALSYVPEAENCGWLKDKYGVSWQIVPTDMNEMMQTADLEKLARVTEA
                     TLKMKKLDLGELRRVYEG"
     gene            complement(2277..2837)
                     /locus_tag="PLT73_01860"
     CDS             complement(2277..2837)
                     /locus_tag="PLT73_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM19159.1"
                     /translation="MEIRQLLADYSPFNEQEEKDRELMLRYLDQFDDLFKRENQLAHF
                     TASSWVVNRDRTKVLMVYHNIYQSWAWMGGHMDGETDFLATAIRETKEETGIEQVTPI
                     SQELFSLEILSVDGHVKNGKQVGTHVHLNLTYLLEADETQQTSVKPDENSGVAWMGLE
                     EALTKCSEPYMRIIYAKLNDKLNRIQ"
     gene            complement(2868..3332)
                     /locus_tag="PLT73_01865"
     CDS             complement(2868..3332)
                     /locus_tag="PLT73_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19160.1"
                     /translation="MTLSFEEKKAIFDDYEELTAVPVSMNRINYHFNASAVDHKIVVR
                     FLHPNGNAFIYAGYLPKEETDKGYISVLESDEGTIRFLLEKAISFLKKTADGYVEGYS
                     EKWVDRSGDVLLLIYDNPMWSVALKNGQMEGIFKTRDAAVGYLNDEGFSRTN"
     gene            complement(3379..4242)
                     /locus_tag="PLT73_01870"
     CDS             complement(3379..4242)
                     /locus_tag="PLT73_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM19161.1"
                     /translation="MEKCDQIERSLIKKYRKPLWRPFIKAIQEYDLIQNGDKIAVCIS
                     GGKDSMLLAKLMQELQRHGPLRFELVFLAMNPGYSEQNQQQILDNAALMDIPLTVFES
                     AIFDVVENVDSSPCYLCARMRRGNLYQKAQEIGCNKIALGHHFDDVIETILMNMFYGG
                     TIETMMPKLHSRNFEGMELIRPLYHVKEADIIAWKDYHQLTFLQCACRFTEMYKNQPS
                     DLGGFKRKEVKALIGQLRETSPLIDANIFQSVQSVNLEKIISYRDGEGRHHFLEKYAD
                     GAERPQPDMVE"
     gene            complement(4306..5118)
                     /locus_tag="PLT73_01875"
     CDS             complement(4306..5118)
                     /locus_tag="PLT73_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRM19162.1"
                     /translation="MQVFLNNAYLEEDAALISPLSPGFMYGYGVFETIRVSGKQVLFL
                     DQHYTRMVQALSVLGMVCPYEENELQPIVEKLLELNGVSEGFVKVVCSKPTPKKQTQQ
                     KADILILTGTKTYKEEYAKGLKVCLADARRNEFSKIVGIKSMNYADNILEKEAAVQKG
                     FDEAIFLNTHDHVSEGCVSNVFWVKDGIVYTPSLHCGILEGTARARIIKKCAALQIPF
                     QEGAYVLEELLSADEVFVTNALMDVMPVSLLEERHLDLGAYQVVPQLQGNGR"
     gene            complement(5121..6368)
                     /gene="pabB"
                     /locus_tag="PLT73_01880"
     CDS             complement(5121..6368)
                     /gene="pabB"
                     /locus_tag="PLT73_01880"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRM19163.1"
                     /translation="MDSGMDPGKLGRYSFIGFDPERTLTAKAGVDSFEELKALLEDYQ
                     LDYTGELPFIGGAVGYFGYELRHQVERLPKEAHDDVQIPDSFFGIYDGAIVVDHLFGK
                     VHLASPGFFGNPEKWVAEVEKTIVAAEGMRFPLRALELASEARPKLTANMTKDYYLDA
                     IARIKEYIRSGDIYQVNMTQRFQCRTEASPYELYTRLRDINPAPFAAYIDFGMGQLLS
                     SSPERFLQIRNGKVQTRPIKGTRPRGATEQEDSANRQELLESEKDRAELLMIVDLMRN
                     DLGRVCKTGSVKVTEMYHIEDYSTVFQLVSTVEGELEEGLHALDCIKAAFPGGSITGA
                     PKIRAMEVIDEIEPTQRNVYTGSIGYVGFNGDADLNIVIRTILQKGDTAYFQVGGGME
                     YEETLVKAKALIEALQAELPEGE"
     assembly_gap    6409..6418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1567 a         1858 c         1489 g         1726 t
ORIGIN      
        1 gcacgcttgc cgtttcaggt gcaacccgat gatgggccat caccagcgat cccaaccctg
       61 caatcacgat cagcaaggaa ctcaggaagc cgatgtaagg cggcatccgc aaagcaccga
      121 ggatgatcaa tcccaacaat accaaccaga cgcctttatg cagctcgggc catttgaagc
      181 gtttcgccaa ccactgcccg atgaataccg ccacaatgat ccgcgacaga tataaagcaa
      241 tcggatagac ggtaccaagg ataatcgcaa tcggaatgcc gatgacggtg accatcgcca
      301 agatgatcag cggcggcacc accaacaatg tcagcagacc gactcccaac gtcttcagcg
      361 gccgttcagt caatggccgg gctgtatttt gccagaaagc ggggcgccag atccggataa
      421 ggaaccaaat cagcaaagcg ctcgccaaac tccagaggaa ccagacaact gtcatgaacg
      481 gcctgcgcgt ttcccgcatg acagttgttc ccctatccat cgtttcggca aattcccaat
      541 ccgtgtctcc tgcgatcgtt gcaccagtcc cgatgtcagc ttcgtttccg cccttgtagt
      601 tgaggttgcc ggctatgttc gcgccgtcct gaagtttcag actatccgtt tgcagattga
      661 catccccgcc aatttggccg ctgagcacaa tctcggctcc acccccgaag aggttgcgtt
      721 ggataacccc ttcctgcagg atggttgcgc ctgcaacgaa gatatccctg cctacagatg
      781 catcctgacc gatggcgata ttctgaccgg caaagaaaac atcgctttcc gactgcccat
      841 caaaggtgag cgcttgcccc gctccataaa tattgccgat caccgtcccg ttgatggtaa
      901 tcgattgggc agccacgaac agatcgccgt tgatgttgcc gttgatccgg acatcctgcc
      961 cggacacaaa cgtagtccct tcaacatttc cgtccacctt caccacattg cccgcaaaga
     1021 aaccaggccc ttcaatcgtt tcacctgcag ctaaagtatc caaattttcc gcattcatgg
     1081 tagtggccga agcaacacct gaaaatccca acaaaatcac caaaaataac aagttgatgc
     1141 tccagaagaa ccctttccac caaaccttct tattcatctt gcttcctcct ttcaaataaa
     1201 gcgcaagtga ccaattgact atccttcgta aactcttctc agttccccaa ggtctaattt
     1261 tttcattttc agagtcgcct ctgtgacgcg tgccaatttt tcaagatccg cggtctgcat
     1321 catctcgttc atgtccgttg gaacgatttg ccaagatacc ccgtatttat ccttcagcca
     1381 tccgcagttt tccgcttccg ggacataaga aagggcttcc caatagtaat cgatttcctc
     1441 ctgattttcg caattgacga tgaaagagaa ggcttcattg aattcaaaat catgggcctg
     1501 agcactgtcc atggctgcga acgcttggtc ttccaacttg aaagaagcga actttaccgt
     1561 ccctggcgca tccggctcct cacccactcc gtagtaatcc attgcatcga ttgaagtatc
     1621 tgaaaataca gcctcataga aaaggatggc ctcctctgct ttgccgcatt gtttttttgt
     1681 gaacatcaag gttggtgtca atttctgttc tatctcccgt tcgccgacat gcatgacctg
     1741 ccaggacagg ccgtagcggt cttctgtcca accatacctt ttgctgaagg gataagcgtc
     1801 caacggcatg agcaccgtgc cgccatcgga aagcttttcc caaaggaaat cgacttcctt
     1861 catcgatgcg caatcgattc ggatggaaat ggatggattg aagcggaaat acggacctgc
     1921 cgcaatcgcc atgaaatcct gaccagacaa cgtcatcgaa acaatatcgg ccgttccata
     1981 tggtggtgga ttttggattt ggctgatgtt tgtgattttg gaattatcga acagcgaggt
     2041 atagaattcg gctgcttctg tagcctgcgt atcaaaccaa aaatgcggaa caattttatt
     2101 catatataaa atcctccttg atctttttca taccgatccc gacagtgcaa atggacaaaa
     2161 aaaatagcgg ctgcaccgac ccgccgaaga agttcttcgg cgaagtcgat gctctccact
     2221 gccattatgc cacttttcgt tggcgatgga aaatgagaac catcggcgct gagcggctat
     2281 tgtattctgt tcaatttatc attcagcttg gcgtaaatga tcctcatgta aggctcgctg
     2341 cacttggtga gcgcctcttc caaccccatc caggcgacgc cgctgttttc gtccggtttg
     2401 acgctcgtct gttgcgtttc gtccgcctcc agcaagtaag tcaggttcag atgcacatgc
     2461 gtccctacct gtttgccgtt tttgacgtgc ccgtccacac tcaatatttc gagcgaaaag
     2521 agctcttgcg atatcggcgt tacctgctcg ataccggtct cttccttcgt ttcccggatg
     2581 gctgtcgcca gaaaatcggt ttcgccatcc atgtgaccac ccatccaagc ccaagactgg
     2641 tagatgttat ggtaaaccat cagcaccttg gtacgatcgc gattgacgac ccaactcgaa
     2701 gcggtgaagt gggctaactg attctcgcgc ttgaacaaat catcgaattg atccagatac
     2761 cgaagcatca gctcacgatc tttttcttcc tgttcattaa agggagaata gtccgcaagc
     2821 aattgtctga tttccatgac agccctcctt tcacatgctt ttagcggtta attggttcgc
     2881 gagaaacctt catcattcag ataaccgaca gccgcatcac gtgtcttgaa gattccttcc
     2941 atttgaccgt tcttcaacgc aaccgaccac atcggattat cgtagatcag caacagaaca
     3001 tctccggacc ggtccaccca tttttccgag taaccttcca catagccgtc tgcggttttt
     3061 ttcaggaagg aaatcgcctt ttccaaaagg aaacggatcg ttccttcgtc gctttccaat
     3121 acagaaatat agcctttatc cgtctcttct ttcggcagat agcctgcata aatgaaggca
     3181 ttgccgtttg gatgcaggaa gcggacaact atcttgtggt ccaccgcact cgcattgaaa
     3241 tgataattga tccggttcat cgaaaccggt acagccgtca attcctcgta atcatcaaat
     3301 atcgcttttt tttcttcgaa tgacagggtc attttattca ctcctcgtta atgttctccg
     3361 attttgcgga ggctcggttc attctaccat atccggctgc gggcgttcag ccccatcagc
     3421 gtatttttca agaaaatgat gccttccttc cccatcgcgg taggaaatga tcttttcgag
     3481 gttcacgctc tgcacactct gaaaaatgtt cgcatcgata agcggactcg tttcgcgcag
     3541 ttgcccgatc aatgccttca cctctttgcg cttaaaacct cccagatcgc tcggttgatt
     3601 tttgtacatt tccgtgaaac ggcaagcgca ctgtaggaag gtcagttgat gatagtcttt
     3661 ccaagcgatg atatctgctt ccttcacatg ataaagtggc cggatcagct ccatcccttc
     3721 aaagttccgg ctgtgcagtt tcggcatcat cgtctcgatc gttccgccgt aaaacatatt
     3781 catcagaatc gtctcgatga cgtcatcgaa atgatggccc aaagcgatct tgttgcagcc
     3841 gatttcctgg gctttttggt acaggttgcc cctgcgcatg cgcgcgcaaa gatagcaagg
     3901 ggacgaatcc acattctcca cgacatcgaa aatagcggac tcaaaaacag tcagcgggat
     3961 gtccatcaat gcggcattgt ccaaaatctg ttgctgattc tgttcgctgt agcccggatt
     4021 catcgccaaa aagaccaact cgaaccggag cggaccatgc cgttgcagtt cctgcatcag
     4081 tttggccagc agcatcgaat ctttcccgcc ggagatgcag acggctatct tatcaccgtt
     4141 ctggatcagg tcatattcct ggatcgcttt gatgaagggc cgccataagg gtttccggta
     4201 ttttttgatg aggcttcttt cgatttgatc gcatttttcc atttgataag ggtttccttt
     4261 cttgcaggtt atccggttga ttttaatgtg tatcgtttgc tccggttagc gcccgtttcc
     4321 ctggagctgg ggcaccactt gatatgcccc gaggtccaaa tgacgctcct ccagaagact
     4381 caccggcatg acatccatca aggcattggt gacgaatact tcatccgcac tcaaaagctc
     4441 ctccagaacg tacgctcctt cttggaacgg aatctgtaat gcagcgcatt tcttgattat
     4501 ccgcgctcta gccgttcctt ccaggatacc gcaatgcagc gacggagtgt atacgattcc
     4561 gtccttcacc caaaaaacat tcgaaacgca cccctcggaa acatggtcat gcgtgttcaa
     4621 aaagatggct tcatcaaagc ctttctgaac ggccgcttcc ttttccagaa tgttgtccgc
     4681 atagttcata cttttgatgc cgacaatttt cgagaattcg ttccttctcg catcagccag
     4741 acatactttc aagcctttcg catattcctc tttgtaagtt ttcgttcctg tcaggatcag
     4801 gatatccgct ttctgttgtg tctgtttttt aggggtcggt ttgctgcaga cgacctttac
     4861 gaagccttcg gaaacgccat tcagctccag taatttttcg acgatcggct ggagctcatt
     4921 ttcctcataa gggcaaacca tccccagaac cgacagcgcc tggaccatgc gtgtgtagtg
     4981 ctggtccaga aacaacactt gtttaccgga gacacggatc gtctcaaaaa ccccgtatcc
     5041 gtacatgaaa cccggggaca gtgggctgat caatgctgca tcttcctcaa gataggcatt
     5101 attcaaaaat acttgcattg tcattctcct tccggtaatt ccgcctgcag cgcttcgatg
     5161 agtgccttcg ccttcacaag cgtctcttcg tattccatac cgccgccgac ctgaaaatag
     5221 gcagtatctc ccttttggag gatcgtccgg atgacaatgt tcagatcggc atcgccgttg
     5281 aacccgacat agccgatcga tccggtatag acgttgcgtt gcgtcggttc gatttcatcg
     5341 atgacttcca tcgccctgat tttcggcgca cccgttatcg atccgcccgg gaaggcggct
     5401 ttgatgcaat cgagcgcgtg gaggccttcc tccaactccc cttcgactgt cgaaacaagc
     5461 tgaaaaacgg tgctgtagtc ttcgatatga tacatttccg ttaccttgac gctgccggtt
     5521 ttacagactc gacccaaatc attgcgcata agatcgacga tcatcagcaa ttcggcacgg
     5581 tccttctcgc tttccagcaa ttcttggcga ttggcgctat cctcctgctc ggtagcgccg
     5641 cgtggccggg tacctttgat cggccgcgtc tgtaccttgc cgttgcggat ttgcaggaac
     5701 cgttcaggcg aactgctcaa tagctgaccc atgccgaagt cgatgtaggc ggcaaatgga
     5761 gccgggttga tgtcgcgcaa acgcgtgtac agctcgtagg gcgatgcttc cgtccggcac
     5821 tggaagcgtt gggtcatgtt gacctgatag atgtcgccgg accggatata ttccttgata
     5881 cgcgcgattg catccaaata atagtctttt gtcatgttcg cggtgagctt tggtctggct
     5941 tcactggcca actccaaggc acggagaggg aatctcatcc cctccgcagc cacgatggtt
     6001 ttttccacct cagccaccca cttttccgga ttaccgaaga accccggcga ggcaagatgg
     6061 acttttccaa aaaggtggtc caccacaatc gccccgtcat aaatgccgaa gaagctgtca
     6121 gggatctgga catcatcgtg cgcttccttc ggcaaccgtt ccacttgatg gcgcagttca
     6181 tagccgaagt agccgacagc cccgccgatg aaagggagtt cccccgtata gtccagctga
     6241 tagtcttcca gcaatgcttt caattcttcg aacgaatcta cgcccgcttt ggcagtcagc
     6301 gtccgttcgg gatcgaatcc gatgaaggaa tagcgtccca atttccccgg atccatgccg
     6361 ctgtccagga aaaaaagcag cgattaaatg cgatcgtcga agagacgcnn nnnnnnnnaa
     6421 aaacaatgct tgcggtcgcg gaacaaagaa aagagttcga agctcgaaag cccggtgacg
     6481 atttccttga tcacgatatc agtcgctctt tttttcatcg aaaatcaccc cttcccgctt
     6541 tttttgggcg cgcccataaa agttgcgcaa catatccaaa ccgtgctccg tcagaatcgc
     6601 ctcgggatgg aactgaaccc cctcgatcag ctcttcccgg tgaagaaagc
//
LOCUS       DAOMUJ010000040         6554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3158_length_6554_cov_1.898908, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000040 DAOMUJ010000000
VERSION     DAOMUJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3158_length_6554_cov_1.898908"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="gcvPA"
                     /locus_tag="PLT73_01885"
     CDS             complement(<1..1016)
                     /gene="gcvPA"
                     /locus_tag="PLT73_01885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRM19164.1"
                     /translation="MTEERFRYLPDTAEDEKEMLAVIGVESMEDLFTDIPDEIRLKEA
                     LAIPAAVSESELLKQMKDLAEKNVSAGQMPIFMGAGTYDHYIPSVVDAVISRSEFYTA
                     YTPYQAEASQGELQAIFEFQSMISELTGMEATNSSLYDGFASLSEAVGLAAAVTKRKE
                     IVLSQAVHPNARAVVHTAAKGRGLEVIEVLMEKDVTNFGVTRCLTTPDTAAIVVQYPN
                     FFGSVEDLAVVKKIAEDKGALLIVMANPLALGILEAPGKLGADIVVGDTQPFGLPMSF
                     GGPHCGYFSVNQKHIRKVPGRIVGETVDAAGERAFVLTLQSREQHIRREKATSYMSSN
                     QALNA"
     gene            complement(1018..1413)
                     /gene="gcvH"
                     /locus_tag="PLT73_01890"
     CDS             complement(1018..1413)
                     /gene="gcvH"
                     /locus_tag="PLT73_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRM19165.1"
                     /translation="MADHKNFYYTKEHEWVEKITDETVRIGITEHAAEQLGDIVFVDF
                     VADLDAELAKGDESVTVESVKSVSEVYAPVSGIVTKQNEALADSPEIVNAEPFADGWM
                     YEMSLSDINELDELLSYDEYAAFVAEEEA"
     gene            complement(1478..>2199)
                     /gene="gcvT"
                     /locus_tag="PLT73_01895"
     CDS             complement(1478..>2199)
                     /gene="gcvT"
                     /locus_tag="PLT73_01895"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRM19166.1"
                     /translation="NKSEASISNISDSIGLLALQGPAAIKILKELTDADLDAIENYHF
                     IAEQEVAGIPHVLISRTGYTGEDGFELYLAGEQTALLWEKLLAVGEAYGALPCGLGSR
                     DTLRLESGMPLYGHELSEEITPLAAGLGFAVKTKKASDFIGKASLTAIREDGPKEKIV
                     GFEAIERGIPREGYKVFSSDGAEIGVVTSGTQSPTLKKSIGMALVKSEAAALGTEIQI
                     EIRNKRIQARIVPKPFYKRKK"
     assembly_gap    2200..2209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2210..2593)
                     /locus_tag="PLT73_01900"
     CDS             complement(<2210..2593)
                     /locus_tag="PLT73_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRM19167.1"
                     /translation="MSEPTIGKHTPLFEYYKANDVKLIDFGGWEMPIQFSGILAEHEA
                     VRERAGLFDCSHMGEIEISGDKAEDYLNGLLTNDTSVMTIGQAQYNIMCYPDGGAVDD
                     VILFKMNEERYLLVCNASNTDKVFAW"
     gene            3046..>5651
                     /locus_tag="PLT73_01905"
     CDS             3046..>5651
                     /locus_tag="PLT73_01905"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRM19168.1"
                     /translation="MKKVLVANRGEIAIRVFRALTELGIPTVGVYAQEDEGSVHRFKA
                     DEAYLVGKGKKPIDAYLDIEDLIRIAKDSGADGIHPGYGFLSENIDFARRCQEEGIKF
                     IGPSLEHLDIFGDKIKAKVAAVEAGIQSIPGSDGPVANIDEVIAFAEAYNYPIMIKAA
                     LGGGGRGMRMAFNEQEAREGFERARSEALAAFGNDEIYVEKYIQDPKHIEIQILGDEH
                     GNIVHLYERDCSVQRRHQKVVEVAPCVSMSDEVRHEICNAALQLMKHVGYVNAGTVEF
                     LLEGDQFYFIEVNPRVQVEHTITELITGIDIVQSQILIAQGQDLHKEIGIPQQENIPL
                     IGAAIQCRITTEDPLNNFFPDTGKINTYRSPGGFGIRLDAGNGFQGTVVTPFFDSLLV
                     KLCVHASTFDQAVRKTERSLIEFRIRGVKTNIPFMFNVITHPIFVSGDAKTTFIDTTP
                     ELFEFPKTRDRGNKTMQYIGNITVNGFPGIQKGHKKFYDKPRIPTDIVVPEQKIITAK
                     NILDEKGPAAVSEWIKDQNRVLLTDTTFRDAHQSLLATRIRTNEMQAIAAETQAAIPQ
                     LFSSEMWGGATFDVAYRFLSEDPWKRLKKLRSQMPDTLLQMLFRGSNAVGYQNYPDNG
                     LEEFIRLAAKNGIDVFRIFDSLNWTKQMEKSIQYVRDNDKIAEAAICYTGDINDPTQT
                     KYTIQYYKEMAKELESLGAHIIAIKDMAGLLKPQAAYRLISELKDTVNLPIHLHTHDT
                     SGNGIYTYSEAIKAGVDIVDVAMSAMSGTTSQPSISSLYYALEGSEHAPEINIHNVHK
                     INRYWEDIRDYYEEFEGGIQTTSTEVYNHQMPGGQYTNLQQQANAVGLNDEWDKVKEM
                     YADVNKMFGDIV"
     assembly_gap    5652..5673
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5674..6492
                     /locus_tag="PLT73_01910"
     CDS             <5674..6492
                     /locus_tag="PLT73_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRM19169.1"
                     /translation="PSSKVVGDMALFMVQNKLNEEDIYAKGDTIDFPESVISFFKGDL
                     GQPTGGFPDRLQEIILKGKKAITERPGSLREPIDFVALKAELEEKIQRTASDEDVISY
                     LMYPQIFLDYVAVYDKFGDVTKLDTPTFFHGMRMGEQIEVQIEKGKTLIIKLNSIGEP
                     DSDGMRILYFDLNGQGREIEVKDMSITSTKAIRKKAEPTNKEHVGATMPGSVLEVLVK
                     RGDRVSQGDPVVITEAMKMETMIRSTISGVVDQIYVIDGDQIEAGDLLIEIEAK"
BASE COUNT         1781 a         1633 c         1448 g         1660 t
ORIGIN      
        1 gcattcaagg cttggttcga actcatgtag gatgtcgctt tttcgcggcg gatatgctgc
       61 tccctgcttt ggagcgtcaa cacaaaggcg cgctccccag ccgcatccac ggtttccccg
      121 acgattctgc cgggaacttt gcggatatgc ttttgattca cggagaagta accgcaatgc
      181 ggaccgccga atgacatcgg caaaccgaac ggctgcgtgt ctccgacaac gatatccgcc
      241 cccaacttac cgggcgcttc caatattcca agcgccaacg gattggccat cacgatcaac
      301 agcgcccctt tatcttccgc aatctttttg acgacagcca aatcttcgac cgagccgaag
      361 aaattcgggt actggactac gattgcggcg gtatccggtg tggtcaggca tcgcgtgaca
      421 ccgaagttgg tgacgtcttt ttccatcaac acttcgatga cttcgaggcc acgacctttt
      481 gcggcagtgt ggacgacggc acgagcattc ggatggaccg cttgtgaaag aacgatttcc
      541 ttgcgtttcg taacggccgc tgccaaacca acagcttcgc tcaaggaagc aaaaccatca
      601 tataaggaag agtttgtggc ttccatcccg gtcaattcgc tgatcatcga ttgaaactca
      661 aaaatcgcct gcagctctcc ttgactggct tccgcctgat aaggggtata ggccgtataa
      721 aattcagagc gcgaaatcac tgcatccaca acgcttggaa tataatgatc atacgtaccg
      781 gcgcccatga aaatcggcat ctgtccggca ctgacgtttt tctcggccaa atctttcatt
      841 tgtttcaata gttccgattc ggaaacggca gccggaatcg ccaacgcttc cttcaaacgg
      901 atttcgtcag ggatatccgt gaacaggtcc tccatgcttt cgaccccgat gacagccaac
      961 atttcttttt cgtcttcggc cgtatccggt aaataacgga atcgctcctc agtcatttta
     1021 cgcttcctcc tcggccacga atgctgcata ttcatcgtag ctcaacaatt catccaattc
     1081 attgatatcg ctcaggctca tttcgtacat ccagccgtct gcaaacggtt ctgcattgac
     1141 gatttcaggg gaatcagcta aagcttcgtt ttgtttcgtc actattccag agacaggcgc
     1201 atacacttcc gagacggatt ttacggattc taccgtcaca gactcgtccc ccttagccaa
     1261 ttcagcatcc agatcagcca cgaaatccac gaacacgata tcgccaagtt gttctgctgc
     1321 atgctctgtg atgccgatgc gtaccgtttc atccgtgatc ttttctaccc attcgtgctc
     1381 tttcgtgtaa taaaaatttt tgtggtctgc cattttgatc atccttccgc gcatggcgct
     1441 ggtttatttt tgttttcatt agtagacgtg cccaaaatta tttttttctt ttgtagaatg
     1501 gtttcggaac aatccgagct tggatacgtt tgttgcgtat ttcaatttgg atctctgttc
     1561 cgagggcggc tgcttccgac ttcactaagg ccattccgat acttttcttc aaagtcggcg
     1621 actgcgtacc ggatgtgacg accccgatct ccgctccgtc cgaggaaaag actttatagc
     1681 cttcgcgtgg aatccctctt tcgatggctt cgaaaccgac gatcttttct tttggtccgt
     1741 cttcgcggat tgccgtcaag gatgctttgc cgataaaatc actcgctttt ttggttttga
     1801 ccgcaaatcc caacccagca gccagtggag tgatttcctc agataattca tgtccataca
     1861 gtggcatccc ggactccaat cgaagcgtgt cccgcgaacc gagtccacaa ggcaaagcac
     1921 cataggcttc tccgactgcc agcagttttt cccacagcaa cgcggtctgc tcacccgcca
     1981 gatataattc aaacccgtct tcaccggtat aacccgtcct cgaaatcaag acatgcggaa
     2041 tgcccgccac ttcctgttcc gcgatgaaat ggtaattttc gatggcatcc agatcagcgt
     2101 ccgtcagttc cttcagaatt ttgatggcgg ccggtccttg cagagcgagc agaccgatac
     2161 tgtcggaaat gttgctgata ctcgcttctg acttgttgtn nnnnnnnnnc catgcaaata
     2221 ctttatccgt gttggatgca ttgcagacca gcaggtagcg ctcttcgttc attttgaaaa
     2281 ggataacgtc atcgactgcc ccgccatccg ggtaacacat aatattatat tgagcctggc
     2341 cgatcgtcat cacgctggta tcattcgtca gcagtccatt caggtagtct tctgctttat
     2401 cgccggatat ttcgatctct cccatatgcg aacaatcgaa cagtccggcg cgttcacgga
     2461 ccgcctcatg ctcggcaagg ataccggaaa actggatcgg catctcccag cccccgaaat
     2521 caatcagctt cacgtcattc gctttataat attcaaataa tggcgtgtgt ttgcctattg
     2581 ttggttcact cacgtataaa tccccctttt ctttactgga ttccccattt ttgcgactat
     2641 ttttcgatta ccgtcgcatt aacgtgtaac cgcttcactt ccctttgtta tctattttag
     2701 cacataaaat gagcaaaagc ctctcacaaa atattgttcg tattagtcgc aaaatcaatc
     2761 tcataatagc acgtactatt tccaattacg aaaaataagc ttatgttatt gatattattt
     2821 tgaataatcg ttcgtatttc gttcaaaaac agcatttccg ccaattatta atggattctt
     2881 aaaaaggtag ttgtaaacaa gctaaataat gtgtaaaatg aataattaaa aagatataat
     2941 tgatttctct cgccaaattt tcatattcgg cagaaatcac gaatcatttt taatcattgt
     3001 ataagaaatt ttcctgaatt ttaataaatt agcgaggtgg aaatattgaa aaaagtttta
     3061 gttgcaaatc gtggcgaaat cgcgatacgt gtatttcgtg cactgactga actgggaatc
     3121 cctactgtgg gcgtatatgc gcaagaggat gaaggttctg tgcaccgttt taaagcagat
     3181 gaagcctatt tggtcggaaa aggcaaaaag cctatcgacg cctatctgga catcgaagac
     3241 ctgatccgca tcgctaaaga ttccggtgca gacggcattc atcctggata tggtttctta
     3301 tctgaaaata tcgacttcgc tagacgctgc caagaagaag gcatcaaatt catcggtcct
     3361 tctttggagc atttggacat ttttggcgat aaaatcaaag caaaagttgc cgctgtggaa
     3421 gccggcatcc aatcaatccc tggatctgac ggtcctgtag cgaacatcga tgaagtcatt
     3481 gcgtttgcgg aagcctacaa ctacccgatc atgatcaaag cggctttagg cggcggcgga
     3541 cgcggtatgc gtatggcctt caacgaacaa gaagcccgtg aaggtttcga aagagcccgc
     3601 agtgaagcgt tggccgcgtt cggcaacgat gaaatttatg ttgaaaagta catccaagat
     3661 cctaaacata tcgaaattca aattttaggg gatgagcacg gcaacatcgt tcacctttac
     3721 gagcgtgact gctctgtgca acgtcgtcat caaaaagtag tcgaagtggc accttgcgtt
     3781 tccatgtcgg atgaagtccg tcacgaaatc tgtaacgctg ctcttcaatt gatgaaacac
     3841 gttggctatg tgaatgctgg aaccgttgaa ttccttttgg aaggcgatca attctacttc
     3901 atcgaagtga atccgcgtgt ccaagtggag cacacaatca cagaattgat tacaggaatc
     3961 gatatcgtcc aatcacagat cctgatcgct caaggacaag atctgcataa agaaatcggt
     4021 atcccgcaac aggaaaatat cccgttgatc ggtgctgcta tccagtgccg gatcacgacg
     4081 gaagatccgt tgaataactt cttccctgat accggaaaaa tcaatacgta ccgttctcca
     4141 ggcggtttcg gtatccgatt ggatgctggt aacggattcc aaggtacggt cgtaacaccg
     4201 ttctttgact ctttactagt taaattatgc gtacatgctt cgacattcga tcaagctgtc
     4261 cgcaaaacgg agcgttcgct gatcgaattc cgaatccgtg gcgtaaaaac gaacattcca
     4321 ttcatgttca acgtcatcac gcacccgatc tttgtatcag gcgatgcgaa aacgacattc
     4381 atcgatacga cgccggaatt gttcgaattc cctaaaacgc gtgaccgtgg taacaaaacg
     4441 atgcagtaca tcggaaacat taccgttaat ggcttcccgg gtatccaaaa aggccacaag
     4501 aagttctacg ataagcctcg gattccgact gacatcgttg ttccggaaca aaaaatcatt
     4561 accgccaaaa atatcttgga tgaaaaaggc cctgcagccg tgagcgaatg gatcaaagac
     4621 caaaaccgcg ttctcttgac ggatacaact ttccgtgatg cgcaccaaag tttgctggct
     4681 acccgtatcc gcacaaatga gatgcaagcc atcgcagccg aaacgcaagc ggccattccg
     4741 caattgttct cttcggaaat gtggggagga gctaccttcg atgttgccta ccgcttcttg
     4801 agcgaagatc cttggaaacg attgaagaaa ctgcgctcgc aaatgccgga tactttattg
     4861 cagatgcttt tccgtggatc caatgccgtc ggataccaaa actatccgga caacggttta
     4921 gaagagttca tcagacttgc cgctaaaaac ggaatcgatg ttttccgtat cttcgacagt
     4981 ttgaactgga cgaaacaaat ggagaagagc atccagtatg tgcgcgacaa tgataagatt
     5041 gctgaagcag ccatctgcta cactggcgat atcaatgatc cgacacaaac aaaatacacc
     5101 atccaatact acaaagagat ggccaaagag cttgaatcac tgggtgcaca catcatcgca
     5161 atcaaggata tggctggcct tttgaaaccg caagcggctt accgtctgat cagcgaattg
     5221 aaagataccg tcaatctgcc gatccatctg catacacatg acacaagcgg taacggcatc
     5281 tacacatact cagaagcaat caaagctggc gtagacatcg tagatgtggc catgagtgcc
     5341 atgagcggca cgaccagcca accaagcatc agcagcttgt actatgccct ggaaggttct
     5401 gaacacgcgc cggaaatcaa tatccataac gtacacaaaa tcaaccgtta ttgggaagat
     5461 atccgtgatt actatgaaga attcgaaggc ggcatccaga cgacttcaac agaagtctac
     5521 aatcaccaaa tgccgggcgg acaatacacg aacctgcaac aacaggcgaa tgcggtcggc
     5581 ttgaacgacg agtgggataa agtcaaagaa atgtatgctg acgtgaataa aatgttcggc
     5641 gatatcgtta annnnnnnnn nnnnnnnnnn nnnccttcct caaaagttgt cggcgacatg
     5701 gctctcttca tggtccaaaa caaattgaat gaagaagata tctacgctaa aggcgatacc
     5761 atcgacttcc ctgaatccgt catcagcttc ttcaaaggtg acttgggcca accgacaggc
     5821 ggattccccg atagattgca ggaaatcatc ctgaaaggca aaaaagccat cacagaacga
     5881 ccaggcagcc tgcgtgagcc gatcgacttc gtggctttaa aagccgaact ggaggaaaaa
     5941 atccagcgta cagccagcga tgaggatgtc atcagctacc tgatgtatcc gcaaatcttc
     6001 ctggattacg tggccgtcta cgataagttc ggcgatgtca ctaaacttga cacccctact
     6061 ttcttccacg gcatgcgtat gggcgaacag atcgaagttc agatcgaaaa aggtaaaact
     6121 ttaatcatta aattgaactc gatcggtgaa ccggattcgg acggaatgcg tatcctttac
     6181 ttcgacctga atggccaggg ccgtgagatt gaagtcaaag atatgtccat caccagcacg
     6241 aaagcgatcc gcaaaaaagc ggaacctacc aacaaagaac acgtcggcgc aacaatgccg
     6301 ggttctgtat tggaagttct cgtcaaacgc ggcgaccgcg tttctcaagg cgatccagtc
     6361 gtgatcactg aagcgatgaa gatggaaacg atgatccgtt caacaatcag cggtgtcgtc
     6421 gatcagatct atgttatcga cggcgatcag atcgaagccg gagatctgct aatcgagatc
     6481 gaagcaaaat aaaaatatag aaaagcggag cgggttcgtt cagccctgaa agaattttag
     6541 gaaatcttac cgac
//
LOCUS       DAOMUJ010000041         6401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3274_length_6401_cov_2.199023, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000041 DAOMUJ010000000
VERSION     DAOMUJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_6401_cov_2.199023"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..975)
                     /gene="heR"
                     /locus_tag="PLT73_01915"
     CDS             complement(85..975)
                     /gene="heR"
                     /locus_tag="PLT73_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HRM19170.1"
                     /translation="MVKGILIEEQFDSTNENGQLALFSSHFRKEAIIMKEITTEKLRR
                     FNLIMGGLHLIQGITMLFLATNVIQKIGEFSPEISQFYLAFNPETRSLETASRVLFEL
                     PFGVMVASFLFISAIAHALVSIPKKLNEIYNADLVKGINKFRWFEYALSSSIMIVLIA
                     TLFGIYDIASLILIFIVNATMNLFGLVMEQLNVGRSKDNIEWGPFIWGSIAGVAPWIA
                     ILLYMFGTGNFGEVPWFVWAIVGTYFVAFNTFPINMILQYKKVGKWADYLYGERVYIV
                     LSLVAKSIIAWLVLFGAMQP"
     gene            complement(968..1597)
                     /locus_tag="PLT73_01920"
     CDS             complement(968..1597)
                     /locus_tag="PLT73_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRM19171.1"
                     /translation="MARNGKKAEPLPIILLVLFVIAYIISAIRPLDRLAWIGQMTPAV
                     LLVLLLVVTYRKFRFSTFVYVMAFLHALLLLYGAHYTYSQNPLFNQWKEQFGWERNYF
                     DRVGHFAQGFVPAFMAKEFLLRGGYVKKGKLLMLIVILSCLGFSAAYELSEFAVVKIM
                     DVPADTVMGTQGDAFDSLWDMIWALIGASLAVFVFGPFHDSQMEQMWDG"
     gene            complement(1613..>2914)
                     /locus_tag="PLT73_01925"
     CDS             complement(1613..>2914)
                     /locus_tag="PLT73_01925"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRM19172.1"
                     /translation="YWMQSSQRAEYNHALEYAIRQANRLEKPLVVYFGITDGFPEANE
                     RHYAFMLEGLKETKAALEQRGIQMLIRKISPEQGALEISELAALLVVDRGYLRIERQW
                     RATLAEGAECAVVQIESNVVVPVELASPKEEYSAATLRSKLKKILPHCLAPLEETICQ
                     HPSVAAELPFEAYDVSDTEKALAGLAIDRSVPRVSDYIGGTTVAKRWLEDFIENKLAG
                     YSERKNHPGESFTSNLSPYLHFGQISPLYIYRRLMGMDSESQQSFLEELVVRRELAVN
                     FVYYNEQYDSYAAIPDWAKKTLDKHLADEREYLYSVEELEGAKTHDDFWNAAQLEMNL
                     TGKMHGYMRMYWAKKILEWSSTPEEAYRKAIYLNNKYLLDGRDPNGFAGVSWCFGKHD
                     RPWGERAIFGNVRFMNDKGLKRKFDMQLYLEQVAERARKRN"
     assembly_gap    2915..2924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3459..3881)
                     /locus_tag="PLT73_01930"
     CDS             complement(3459..3881)
                     /locus_tag="PLT73_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM19173.1"
                     /translation="MKDENCAYCMEGEMVLAFGYPCCELPYSKVYVFKEQSHKGRVIV
                     AYKDHVSELVNITDEERNGYFADIATVANALHKAFSPEKVNYGAYGDTGKHLHFHLVP
                     KYKDDAFEWGSTFDMNPGRVSLTDDAYEALAEEIRINL"
     gene            complement(3997..>4188)
                     /locus_tag="PLT73_01935"
     CDS             complement(3997..>4188)
                     /locus_tag="PLT73_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19174.1"
                     /translation="AGILRLLGSLIAKKPFKDCFYLKGRSFAIYAVLLFFPGTPFLIL
                     VHLTAWAMVGYVEKKHHLN"
     assembly_gap    4189..4198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4235..4462)
                     /locus_tag="PLT73_01940"
     CDS             complement(4235..4462)
                     /locus_tag="PLT73_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19175.1"
                     /translation="MIKNNKRRCTFFFIMSVLLFIFSGCGSASDEAASSSDPELIRES
                     QISEDQSAGYGYVPEYQVLEKILEFFGYSEE"
     assembly_gap    4676..4685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4797..5902)
                     /locus_tag="PLT73_01945"
                     /pseudo
     CDS             complement(4797..5902)
                     /locus_tag="PLT73_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185159.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
     gene            complement(6132..>6401)
                     /locus_tag="PLT73_01950"
     CDS             complement(6132..>6401)
                     /locus_tag="PLT73_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HRM19176.1"
                     /translation="DTSLKGTQKQIEDYADTAWKTRKENIVDNQDQPGDPDKAGEVIY
                     ETMQKETLPKRLLLGSDAVKIVSAEMKERLQEIEDWSAISTQTDY"
BASE COUNT         1621 a         1713 c         1372 g         1665 t
ORIGIN      
        1 catccaatat acaccgtcat tgtaaatcgt atcaaaaaat cctgtattgt ttttgtaaat
       61 gacagcgcct gaagcctacc aaaattacgg ctgcatcgct ccgaaaagca caagccaggc
      121 aataatcgat ttcgcaacca gactgagcac gatgtagacc cgctccccgt agaggtagtc
      181 cgcccatttc ccgacttttt tgtactgcaa aatcatgttg atcgggaaag tgttgaaagc
      241 aacgaaataa gtaccgacga ttgcccagac aaaccaaggc acttcgccga aattgccggt
      301 cccgaacatg taaagcaaaa tagcgatcca tggcgcaaca ccggcaatcg agccccaaat
      361 gaacggtccc cactcaatgt tgtcttttga gcggcccaca ttcaactgtt ccatcacaag
      421 cccgaaaaga ttcatggtgg cgttgacgat gaagatcaga atcagcgaag cgatatcata
      481 gatgccgaac agcgtcgcaa taaggacaat catgattgac gagctgagcg catactcgaa
      541 ccagcggaat ttattgatac ccttcaccaa atcagcatta tagatttcat taagcttttt
      601 cggaatggaa accaaggcat gcgcgatggc tgaaatgaac aagaacgaag cgaccataac
      661 tccaaatggt aattcaaaaa gaactcgcga ggcagtctcc aacgagcgcg tttcaggatt
      721 gaaggccaag tagaattggc tgatttcagg actgaactca ccgattttct ggataacatt
      781 tgttgccaaa aagagcattg tgataccttg aatcaggtgg agtccaccca tgatcagatt
      841 gaagcgccgt agtttttctg tagtgatctc tttcatgatg atagcttcct ttctaaaatg
      901 actggaaaat aatgccaatt gaccgttctc attcgttgaa tcaaactgtt cttcaatcag
      961 aatgccttta accatcccac atctgttcca tttggctgtc gtggaacggg ccgaacacga
     1021 acaccgccag gctcgcaccg atcaaggccc aaatcatatc ccagaggctg tcgaaggcgt
     1081 ctccctgtgt ccccatcacg gtgtcggcgg gaacatccat aattttaacg accgcgaatt
     1141 cactcagttc gtaggctgcg ctgaagccga ggcaggacag aataacgata agcatcagca
     1201 gtttgccttt cttgacgtag ccgccccgca acaaaaactc cttggccata aaagccggca
     1261 cgaatccttg ggcgaagtgc cccacccggt cgaagtaatt gcgctcccaa ccaaactgct
     1321 ctttccattg attgaacagc ggattctggg agtaagtgta gtgcgcgccg tacagcaaca
     1381 acaaggcatg cagaaaagcc atcacataga cgaaagtgct gaaacggaac ttccggtaag
     1441 tcacgaccag caataatact aacagaactg ctggcgtcat ctgacctatc caagccagcc
     1501 gatccaatgg acggatagcc gaaataatgt acgctataac gaaaagaacc aaaaggatta
     1561 taggcaaggg ttctgccttt ttgccgtttc tcgccatccg ttccgcctcc ttttagttgc
     1621 gtttgcgtgc ccgttccgca acctgttcca gatacagttg catatcgaac ttgcgcttca
     1681 ggcctttatc attcatgaac cggacattgc cgaagatggc acgttccccc cacggccggt
     1741 catgcttgcc gaagcaccag ctcactccgg caaacccgtt cggatcgcgg ccgtccaaca
     1801 ggtacttatt gttcagataa atagcttttc gataggcttc ctccggcgtg ctgctccact
     1861 caagaatttt cttggcccag tacatccgca tgtacccatg cattttaccg gtcaggttca
     1921 tctccagctg tgcggcgttc caaaagtcgt cgtgggtctt tgccccttcc aattcctcca
     1981 cagaatacag atattcgcgt tcatccgcca agtgcttgtc cagtgtcttc ttggcccaat
     2041 ccggaatagc tgcataggaa tcgtactgtt cattgtagta aacgaaattg acggccaatt
     2101 cccttctgac aacaagttcc tcgaggaaac tttgctgact ttcgctgtcc atccccatca
     2161 gtctgcggta gatatagagc ggtgaaatct ggccgaaatg gagatatgga cttagatttg
     2221 aggtgaagga ctcgccggga tggttcttac gttcgctgta cccggccagt ttattctcga
     2281 tgaaatcctc caaccagcgt ttggccaccg tggttccgcc gatataatcg gaaacgcgcg
     2341 ggacactacg gtcgatagcc aagccggcca aggccttttc ggtatcggaa acgtcatagg
     2401 cctcgaacgg aagctccgct gctacggatg gatgttggca gatggtctcc tccaatggcg
     2461 ccaaacaatg cggcaggatt ttcttcagct tggagcgcag ggtcgcggcg gaatattcct
     2521 ctttcggaga agccagttcg accggaacaa cgacattgct ttcaatctga acgacggcac
     2581 attcagcacc ctcggccaag gtagcgcgcc attgccgctc gatccggaga taaccgcggt
     2641 cgaccaccaa cagggccgcc aattcggaaa tttccaatgc tccttgttcc ggcgatattt
     2701 tccggatcag catttggatg ccgcgttgct ccaaggcggc cttcgtttcc ttcagccctt
     2761 ccagcatgaa tgcatagtgg cgctcattgg cttccggaaa accgtccgta atcccgaagt
     2821 aaacgaccag cggcttctcg agcctgtttg cctggcggat cgcgtattcc aaagcatggt
     2881 tgtactccgc ccgctgcgag ctctgcatcc aatannnnnn nnnngacata gggtcgctcc
     2941 atcgctgttt tcttgtcact cagttgcttg attctttctt ccagtatcat ccggttcccc
     3001 ccaatttgtg agcgtttgtt gtcatactta ttatagaaca tcgctccgac tcctactaat
     3061 tttacgaatc aaatcaaact gaaacggtgc cctttttgag cggaacaaac aatatagtct
     3121 gcagaaaaaa tgctgaaacg cacaaacagc cgatggggcg ttctgtcggc tgtttatgcg
     3181 ttttctatac ctcttgtcgg ctgttccaca tccgctccct agagatcagc cgacgaagtg
     3241 agcacccgtt gactgttcgt ccttcatttt tccgacaata gtcgacggcc gcagcttatg
     3301 ttgactgttc cgcattcact ttttccgcaa cagtcgacgc ctgaagcgct tgtcggctat
     3361 tccgcaaata tcctgccctc aacagtcgac gagtccgcgc aaaaaagctg attccgtttt
     3421 tggaatcagc ttcttagaaa gaaaattttt acatttcctt atagattgat gcggatttct
     3481 tcagccaatg cttcatacgc atcatccgtc aggcttacgc ggcctggatt catgtcgaaa
     3541 gtcgagcccc attcgaaggc atcatctttg tattttggca ccaagtggaa gtgcaggtgt
     3601 ttgccggtat cgccgtatgc accgtagttt actttttcag ggctgaaggc tttgtgcaac
     3661 gcgttcgcaa ctgtagcgat atctgcgaag taaccgttgc gttcctcatc cgtgatgttc
     3721 accaattcgc tcacatggtc tttgtaggca acgatcacac ggcctttgtg gctttgttct
     3781 ttaaaaacgt agactttact gtatggcaat tcgcagcaag gatatccgaa agccaacacc
     3841 atttcgcctt ccatgcaata agcgcaattt tcatctttca tcaacacaga cctcctagaa
     3901 atatacttcc ggtccattat agcactttta agcgcctaca ttttgtccat aattcaacag
     3961 tattgcgtac aaaatgtgaa cacactggat ctctactcaa ttcaagtggt gcttcttttc
     4021 cacatacccg accatggccc aagctgtcag atgcaccaaa atcagaaatg gggtccccgg
     4081 aaaaaacagc agcaccgcat agatggcaaa agaccgcccc ttcaaataaa aacagtcttt
     4141 aaatggtttc ttcgcaatca aacttcccaa aagccgaagg ataccagcnn nnnnnnnntc
     4201 atctcttcac ctccaaatcc attccataaa aatatcattc ttctgaatag ccaaaaaact
     4261 ctaaaatttt ctccagcact tggtattcgg gtacataacc ataccctgcg gattgatctt
     4321 cgctgatttg gctctccctg atcaattccg gatccgaaga tgaggcagct tcatcgctcg
     4381 ctgaaccgca tcccgaaaag ataaacaaca acacggacat gatgaagaaa aacgtgcatc
     4441 ggcgtttgtt atttttgatc atctgaagtc cctccttgga ctaaaaaaac tgatgacaaa
     4501 ggaagacaat cgactataaa gcctattttg taaccgctct ttttcgactt cattataaca
     4561 tatcacgagc acctgaatcg cgtaaggctt caccctgata caatgcaaaa agaccacaca
     4621 gaacttccat tccagaaggt ctgtatggtc ttatctaaat tgtttattta cctaannnnn
     4681 nnnnnattat aacatatcac gagcgcctga atcgcttatg ccctcaggta acacacaaga
     4741 aaaagaccat acagaagtcc cttttcccag gaagtctgta tggtccaact aattgtttat
     4801 ttacccaagc ggtccggcat catgaactcc atgaagcccc agacgccgtt cgcgaggaac
     4861 agttcttcgc ggtcgtaatt ggatatttct tggcggatca gatcatcctt ttagcgaggg
     4921 ctttcgcgcc ccaatgcgga tcgatcagca aagcacgtcc aacggcaacc agctccgaat
     4981 tttccaatac ttgctccgca tcttcgctcg tgcggacatc gccgacgccg accaacggca
     5041 cgcgtccgtt aatttgttca tgaatgtacg ccatcatgct tttctcttta tgatcctcag
     5101 aaacggaaac cagttgatgg tctctcaagg aaatatgcag ataatccaag cctttatcgg
     5161 ccaatttgtc cacaaggaac agtgtatctg taaagcggat accaggattt tcatattctt
     5221 caggggaaaa gcggtagcca acgataaagt tctctgcgcc cgattcatcc acggttgctg
     5281 tcacttcatc aaccaatttg tcgatgaaac ggaaccgttt ttccagcgtg ccaccccatt
     5341 cgtcgtctct gcggttggag tgcggcgaga agaattgttg caacagataa gtattggcac
     5401 catgcagttc cacaccatca aaaccggctt cgatcgcgcg acgggtcgcg gctttgaagt
     5461 tttcgatcaa gtccagaact tcgtcaccgg ccaatgctct cggcgtttcg gcattcggtc
     5521 tgtcagcagc aatcgcactt ggcgctactg gctgaacgcc acgcaaaacg cttgaatccg
     5581 tcatgcggcc accgtggaaa atctgcagga tggctttggt gccgtttttc ttgatggctg
     5641 cagccaattt gctcaaaccc ggaatgaaca catcggaagc gacgctcaat tcgccttccc
     5701 agcctttgcc gtcattctga acatttgcag cgcctgtgat gaccgcgcct atttcaccgg
     5761 aacgaagcgc atagtaattg atctcgtcgg ttgtcactac cccatcatag aaactcattt
     5821 tcgtcgtcat cggtgccatc atgatgcggt tcttcagttc caggccgcgt ttgaaagtca
     5881 gtttactgtt cattgttgac atattttttc tctccccttg gatcgcttag ttttattcta
     5941 ccgagcttgc cggaaattcc ctaacaaaat gctcggccgc ctcaaattgc atgcgcatgc
     6001 atatatttta ttacaagcac tcgcttttgg caagttttac actgatccgt cacgcaaaaa
     6061 gacagcggcg gcaatcatgc ccgcctccgc tgtcttgcat catttatttt tttatattcc
     6121 caataatccg atcagtaatc cgtttgcgtg ctgatggcgc tccagtcctc gatttcctgc
     6181 aatctttcct tcatctcagc agacacgatt ttcaccgcat ccgaaccaag caacaaccgt
     6241 ttcggaaggg tttctttctg catggtttcg taaatgactt cgcccgcttt gtcaggatca
     6301 ccgggctggt cctggttgtc gacgatgttc tcttttctgg ttttccaagc ggtatcggca
     6361 taatcttcga tctgcttctg ggtccctttc agggaagtgt c
//
LOCUS       DAOMUJ010000042         6313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3349_length_6313_cov_2.387824, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000042 DAOMUJ010000000
VERSION     DAOMUJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_6313_cov_2.387824"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLT73_01955"
     CDS             <1..580
                     /locus_tag="PLT73_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRM19177.1"
                     /translation="DATTFELDNRLYYVWAQKDPAIPGNSNLYISEMQNPWTLCGKQT
                     LLSIPEYAWEKVGFLVNEGPAVIIRNGKVFITYSGSATDENYCMGLLWADSASDLLDG
                     YNWHKLAEPVFRSSEQNQLFGPGHNSFTRSEDGEDDVLVYHVRPKIVTEGDPLDVPDR
                     HAHAQVFTWSADGFPIFGEPGTDNNQKGRTII"
     gene            577..2028
                     /locus_tag="PLT73_01960"
     CDS             577..2028
                     /locus_tag="PLT73_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HRM19178.1"
                     /translation="MNKIQVTLNRETGTISRYIYGQFAEHLGRCIYEGVWVGTDSEIP
                     HVNGIRQDVVEALKAIQVPVIRWPGGCFADEYHWKDGIGPTEERATIVNTHWGGVTEN
                     NHFGTHEFFELCRQVGAEAYINGNVGSGTVQEMQEWVEYMTMDGLSPMSKLRRENGQD
                     EPWKVKFFGVGNESWGCGGNMRPEYYADLYRRYQTYVRQYTKEPLYKIACGPNIDDYN
                     WMDVLMKHAAPFMDGISLHHYALASVWEDKRPALGFPEKEWFSLIDSALKMDELITRH
                     TTIMDKYDPDKRVGLIVDEWGSWLAVEPGTNPGFLYQQNTIRDAMIASLTLNIFHKHA
                     DRVQMANIAQMVNVLQAMILTEGAEMIKTPTYHVFDLYKNHQDATLVDADGVISGTTS
                     YTVSKKDGVMTASFCNYSLTDTETITLSRVDGVFGETAAEGIFGNSTDDHNSFENPEA
                     VAKQTFTDFAVDGNELTITLPPMSVVSVYAKEN"
     gene            2030..2266
                     /locus_tag="PLT73_01965"
     CDS             2030..2266
                     /locus_tag="PLT73_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HRM19179.1"
                     /translation="MTCKGCERNNQMMEMDIESAIDEQLSMELNHASNELRDQRLFVC
                     SACPALSEHTCKHCGCFVRFRASLQDKRCPLGKW"
     gene            2397..4385
                     /locus_tag="PLT73_01970"
     CDS             2397..4385
                     /locus_tag="PLT73_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRM19180.1"
                     /translation="MIKPLLQQHPRVTVSDSFWLKYRQLVREEMIPFQWDVLNDRGNI
                     VIESEREDATIPTEKSHVIENFRIAAGQKEGHHYGWLFQDSDLYKWIEAAANTIALEK
                     DEALVAQVEETIELLEAAQDDDGYLSTYYQIDAPHLKFRRLFESHELYCIGHLIEAAI
                     AYKEATGSGRLLQIADKAIDCIEAHFGKSEGKIDGSDGHQEIELALVRLYESTGNRKY
                     LDLSSYFLEVRGQNPHFFAEQLEENDRLGLQQGPKPVINTVYHQAHKPVIEQDSARGH
                     AVRLVYMAQAMAGAGRHKQDEKLIGAAKKIWENIVQKRMYITGGIGSTVRGEAFTYDY
                     DLPNDLMYCETCAAIGLLNFSNELLKTETDSQYADIMERTLYNSIISGMALDGKHFFY
                     VNPLEVDPQASAENPDKSHVKATRPSWFGCACCPPNLARTLPAVGGYAFTLKEDEVLL
                     NLFIDSELAGEQAGEPYTIRQKHTVSETGQTVIAVEKASSEQLRLGIRIPYWAENPVL
                     VVDGQETVAESSNGYTYVTVISDSMAIELTYTIAINEIEAHPLVKADKGKVALQRGPF
                     IYCLEEQDNGKQLHLLALTGSVRPRFRSDKLLGDSVFLEAEGELQVMEDGWQGKLYRV
                     HQKPQTIPKMLTFIPYYNWGNRSLGEMQVWVDKEDTHV"
     gene            4378..5649
                     /locus_tag="PLT73_01975"
     CDS             4378..5649
                     /locus_tag="PLT73_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM19181.1"
                     /translation="MFRSYAETPPKGWNSWDSFGASVTEEEVLGNAEVMAKSLKDYGW
                     EYIVVDIQWYEPLADSCAYHPFTELRQDEYSRLIPAENRFPSSKGGLGFKPLADAVHA
                     MGLKFGIHTMRGIPRQAVHGNSPILGTQKTARDIADTNSICPWNTDMYGVDNTKDGAQ
                     AYYDSLLALYASWGVDFLKVDDIADSKLYGTHSGEIELIRKAIDSCGRPMVLSLSPGP
                     APIERGTFFQRNANMWRLTDDLWDNWEQLYDMFGRCGKWSAFVGTGAWPDCDMLPLGH
                     IGTRTAADGSEARMTRFTKEEQRSLMTLWSIFRSPLLFGGDLRKLDDWTHSLLSEPYL
                     IDAHQNGRDPKEVYRKEDLVIWVSESDTRKYVACFNLADRPVGLPVAAREWLQSLGGE
                     LHEVWANRAVSVADAETAFIAPHGVALYISK"
     gene            5818..>6313
                     /locus_tag="PLT73_01980"
     CDS             5818..>6313
                     /locus_tag="PLT73_01980"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRM19182.1"
                     /translation="MKTVQVKDVVFNEGRPKICVPLVGKTKQEIITEVKMLEKVAFDL
                     AELRIDFFEGVEYPEQVEALLTEINSIYKKPLLFTFRTKKEGGERELSLDHYFELNRF
                     AIRSGLVDMIDLELLSSEEDVINLIAEANEKNVKVVLSSHDFFRTPPQKDIVARLVKM
                     QQLGA"
BASE COUNT         1667 a         1506 c         1801 g         1339 t
ORIGIN      
        1 ggatgctacg actttcgagt tggacaacag actctattat gtctgggccc aaaaagaccc
       61 ggccattccg ggaaattcca atctttacat ttccgaaatg cagaatcctt ggacactgtg
      121 cggcaagcag acgcttctga gtatcccgga atacgcttgg gagaaggtcg gcttcttggt
      181 gaacgaaggg ccggcggtca tcatccgcaa tggtaaagtc ttcatcacct attccggcag
      241 cgcgaccgat gaaaattact gcatgggcct attgtgggcg gattccgcca gcgacctgtt
      301 ggacggttac aattggcaca aacttgcgga accggtcttc aggagttccg aacaaaacca
      361 gttgttcggg cccgggcaca atagcttcac ccgcagcgag gatggagagg atgatgtgct
      421 ggtttaccac gtacgcccaa aaatcgtgac agagggtgat cccttggatg tgcccgatcg
      481 ccatgcgcac gcgcaagttt tcacctggag cgcggatggt tttccgattt tcggcgaacc
      541 cggaacagac aacaatcaga aaggcaggac aatcatttga ataagataca agtaaccttg
      601 aacagagaaa caggaacaat cagtcgctat atatatggtc aatttgcgga gcatctgggc
      661 agatgcatct atgaaggggt atgggtagga accgattcag aaatcccgca cgtcaacgga
      721 atcagacaag atgtcgtgga ggcgctaaaa gccatccagg ttccggtcat cagatggccg
      781 ggcggctgct ttgcagacga gtaccactgg aaagacggca tcggtcctac cgaagagcgc
      841 gccacaatcg tgaatacgca ctggggcggc gtgacggaaa acaaccattt cggtacgcat
      901 gagttctttg aattatgcag acaggtcggg gctgaagcct acatcaacgg aaacgtcggc
      961 agcggcacgg tccaggaaat gcaggaatgg gtcgaataca tgacaatgga cggtctttcg
     1021 ccgatgtcca aattgcgccg tgaaaacggg caggacgagc cgtggaaagt caaattcttt
     1081 ggcgtcggca atgagagctg gggttgcggc ggcaacatgc gtccggaata ttacgctgat
     1141 ctgtatcgcc gttaccaaac ctacgtcaga cagtacacga aagaaccgct ttacaaaatc
     1201 gcctgcggcc cgaacatcga tgattacaac tggatggatg tgctgatgaa gcatgcagcg
     1261 ccgttcatgg acggcatcag cctgcaccat tatgcgctgg cttccgtatg ggaagacaag
     1321 cgcccggcac tggggttccc tgaaaaagaa tggttttcac tgatcgacag cgctttgaag
     1381 atggatgaat tgatcacgcg gcacaccacg atcatggaca aatatgatcc tgataaacgc
     1441 gtcggtctga tcgtggatga atggggctct tggctggctg tggagccagg cacgaacccg
     1501 ggcttcctgt accaacagaa cacaatccgg gatgcgatga tcgcaagtct gactttgaat
     1561 attttccata agcacgctga ccgcgtacag atggcgaata ttgcccaaat ggtcaatgtg
     1621 ctgcaagcta tgattctgac tgagggcgca gaaatgatca agacacctac ctaccatgtg
     1681 ttcgatctct ataaaaatca ccaggacgca acccttgtcg atgcggatgg ggtcatttcc
     1741 ggcacgacca gttacacggt atccaaaaaa gacggcgtca tgacggcatc tttctgcaac
     1801 tacagtctga ctgacacaga aaccatcacg ttgagccgtg tcgacggcgt attcggggaa
     1861 acagcagcag agggcatctt cggaaacagc acggatgatc ataactcgtt cgaaaatcct
     1921 gaagccgttg cgaaacaaac attcactgat ttcgcggtgg atggcaacga attgacgatc
     1981 acgctgcctc cgatgagtgt cgtttccgta tacgcaaagg aaaactgaca tgacctgcaa
     2041 aggctgcgaa cgtaacaatc agatgatgga gatggatatc gagagcgcca tcgatgagca
     2101 actgtcgatg gagctgaacc acgcctcaaa cgagctgcgg gaccagcggt tgtttgtctg
     2161 tagtgcgtgt ccggcgcttt cggagcatac gtgcaagcat tgtggctgct tcgttcgctt
     2221 ccgtgccagt ctgcaggaca aacgctgccc cttgggtaag tggtaacgcc atccagctta
     2281 ggcaccgctt ttgtatcagc gtttatatca acaggaatag ctgccggctt gctggtggct
     2341 tttcttgcat cttgacataa aatcaggttg aaatttggat gaaatgggga tagataatga
     2401 tcaaaccatt gctgcaacaa cacccgagag tgacagtatc agacagcttc tggctgaagt
     2461 acaggcaact ggtgagggag gaaatgatcc cattccaatg ggatgtcctc aacgacagag
     2521 ggaacatcgt catcgaaagc gaaagggagg atgcgaccat cccgacggaa aaaagccatg
     2581 tgatcgaaaa tttcaggatt gcggctggac agaaggaagg acaccattac ggctggcttt
     2641 tccaggacag cgatctgtac aaatggatcg aagcggcagc caacacaatc gcgttggaaa
     2701 aggatgaagc tctggtcgcc caagtggagg aaacgatcga actcttggaa gcggcgcagg
     2761 acgatgacgg gtatttgagt acctattatc agatcgatgc cccgcacctg aagttcagaa
     2821 ggttgttcga aagccatgaa ctgtactgca tcggtcatct gatcgaagcc gccatcgctt
     2881 ataaggaagc gaccgggtcc ggtcggctgc ttcagattgc cgacaaagcg atcgactgca
     2941 tcgaagcgca tttcggcaaa tcggaaggga aaatcgacgg tagcgatggc caccaggaaa
     3001 tcgaactcgc gcttgtgcgg ctgtacgagt cgacgggcaa ccggaaatac ttggatttga
     3061 gcagctactt cctggaggtg cgcggacaga acccacattt ctttgcggag caactggaag
     3121 aaaatgatcg cttgggactg cagcaagggc cgaagcctgt catcaatacc gtctatcatc
     3181 aagcgcataa gcccgttatc gagcaggatt cggcaagagg gcatgccgtt cggttggtct
     3241 atatggcgca ggcaatggct ggggccggtc gccacaagca agatgaaaag ctgatcggtg
     3301 ccgcgaaaaa aatctgggag aatatcgtcc aaaagcgcat gtacatcacg ggtggcatcg
     3361 gctcgacggt ccgcggggaa gctttcacct atgattatga tttgccgaac gatctgatgt
     3421 actgcgaaac atgcgcggcg atcgggctgc tgaatttttc gaatgaattg ctgaagaccg
     3481 aaaccgacag ccaatatgcg gacatcatgg agaggacact ttacaacagc atcatcagcg
     3541 gcatggcctt ggacgggaag cacttctttt acgtcaatcc gttggaagtc gatccccaag
     3601 cgagcgccga gaatccggat aagagccatg tgaaggcgac gcggccttcc tggttcggct
     3661 gcgcctgctg tccgccgaac ttggcgcgca cgctgcccgc tgtcggggga tacgccttca
     3721 cgctaaagga agatgaagtg ctgctgaatc tgttcatcga cagcgaactg gccggggagc
     3781 aagccggcga accttacaca attcggcaaa agcatacggt atcggaaacg ggccagaccg
     3841 taatcgcagt tgaaaaagca tcctccgaac agctgcgttt gggcatccgt atcccttatt
     3901 gggcggaaaa tccggtactg gttgtggacg gtcaagaaac ggttgccgaa agcagcaatg
     3961 gctacactta tgtgaccgtc atctccgatt cgatggctat cgaattaaca tacaccatcg
     4021 ccatcaacga aatcgaggcg catccacttg tcaaagcgga taagggaaaa gtcgccctgc
     4081 aacggggacc tttcatttat tgcctggaag aacaggacaa cgggaaacaa ctgcatctgc
     4141 tggcattgac cggatcggtc cgaccacgtt tccgatcgga taagctgctc ggcgatagcg
     4201 tcttcctgga agctgaaggg gagctgcaag tgatggaaga tgggtggcag ggcaagctgt
     4261 accgcgttca tcagaaaccg caaaccattc ccaaaatgct cacgttcatc ccctactaca
     4321 actggggtaa tcgctctttg ggagaaatgc aagtatgggt cgataaggag gatacgcatg
     4381 tttagaagct acgcagaaac accaccaaaa ggttggaaca gctgggattc tttcggagcc
     4441 agcgtgacgg aagaggaagt attgggcaac gccgaagtga tggcgaaaag tttgaaggac
     4501 tacggttggg aatacattgt cgtggatatc cagtggtatg aacctctcgc tgattcctgc
     4561 gcctatcatc cgttcaccga actccgtcag gatgaataca gccggctgat ccctgccgaa
     4621 aaccgattcc cttcgagcaa agggggacta gggttcaaac cattggcgga tgcggtccac
     4681 gcaatgggtc tgaaattcgg catccatacc atgcgcggca tcccgaggca ggcggtgcat
     4741 ggcaatagcc cgattttggg tacgcaaaaa acagcccggg acattgcgga tacaaattcc
     4801 atctgcccgt ggaacacaga tatgtacggc gtggacaaca cgaaggacgg tgcacaagcg
     4861 tactacgatt cgttgctggc tctgtacgct tcctggggag tcgattttct gaaagtagat
     4921 gatatcgcgg attcgaaatt gtacggaacg cacagcggag aaatcgagct gatccgcaaa
     4981 gccatcgaca gttgcggcag accgatggtg ttgagcctct caccgggacc cgccccgatc
     5041 gaacggggta cctttttcca acggaatgcc aacatgtggc ggctgacgga cgatctgtgg
     5101 gacaattggg agcagctgta cgacatgttc ggacgctgcg gaaagtggtc ggcttttgtc
     5161 ggcaccggtg cttggccgga ttgcgatatg ctaccgttgg gccatatcgg cacccggacc
     5221 gcggctgacg gttcggaggc gcgtatgaca cggttcacga aagaggagca gcgttctttg
     5281 atgacgctgt ggtcgatttt ccgttcgccg ttgctgttcg gcggcgatct gcgtaagttg
     5341 gatgattgga cgcattcgtt gttgagcgaa ccctatctga tcgatgcgca tcagaacggg
     5401 cgcgatccaa aggaagtgta ccgaaaggaa gatttggtga tctgggtttc cgaaagtgat
     5461 accaggaaat atgtggcttg cttcaatctt gcggatcgtc cagtaggctt gcctgttgcc
     5521 gccagggaat ggctgcagtc acttggtgga gaactgcatg aggtatgggc aaacagggca
     5581 gtttcggtgg cggatgcgga aacggctttt atcgctccgc acggtgtcgc tctgtatatt
     5641 tctaagtaaa gacagaacgg aaacgggaac gaatcggaca acttccgtaa agtgctccta
     5701 tgcttttggc gcaattaacg ataaaatggg aagtatcata tactcgtaag tccagctttc
     5761 tttgcccaaa aatagacaag gaagaggacg ggaatgcgcc atggagggga taaaccaatg
     5821 aaaaccgttc aagtgaaaga tgttgtcttc aacgaaggca ggccgaaaat ctgcgtgccg
     5881 ttggttggga aaacaaaaca ggaaatcatt actgaagtca aaatgctgga aaaagtcgct
     5941 ttcgatctgg cggagctgcg catcgatttt ttcgaagggg tagagtatcc ggaacaggtc
     6001 gaagcgttat tgaccgaaat caacagcatt tacaaaaagc cgttgctgtt cacgttccgg
     6061 accaaaaaag agggcggaga gcgcgagctg agcttggatc actattttga attgaaccgt
     6121 ttcgcgatcc ggagcgggct ggtggacatg atcgatctgg aactgctcag ttcggaagaa
     6181 gacgtgatca atttgattgc tgaagcgaac gaaaaaaacg tgaaagtcgt tctgtcgagc
     6241 cacgatttct tccgcacgcc tcctcaaaag gatatcgtcg cgagattggt gaagatgcag
     6301 caactcgggg cgg
//
LOCUS       DAOMUJ010000043         6222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3395_length_6222_cov_2.424355, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000043 DAOMUJ010000000
VERSION     DAOMUJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_6222_cov_2.424355"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1600
                     /gene="pyk"
                     /locus_tag="PLT73_01985"
                     /pseudo
     CDS             179..1600
                     /gene="pyk"
                     /locus_tag="PLT73_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            complement(1733..3124)
                     /gene="argH"
                     /locus_tag="PLT73_01990"
     CDS             complement(1733..3124)
                     /gene="argH"
                     /locus_tag="PLT73_01990"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRM19183.1"
                     /translation="MAIWSNNYHGSMNEKAFEFNSSIDADARLVYADIAGSIAHAKML
                     GKTEIIPREEADALVNELGKMNDELQDGDLTIDFSEEDIHSFLEAELTRRLGAIGKKV
                     HTGRSRNDQVATALKLYSVSALEHLSSLTANVALALSEKAEKHLETIMPGYTHLQRAQ
                     PITFAHHLMAYTEMFLRDKSRLDDAKERVLHYMPLGSAALATTTLPLDRAFTAEALGF
                     KNFSRNSLDGVSDRDHSLEMLADLSIIMVHISRLAEEVIIWSSQEFGFVKIRDTFSSG
                     SSIMPQKKNPDIAELLRGKSGRVFGDLMGVLTLMKGLPLAYNKDMQEEKELLFEAIDT
                     VTFCLEILPAMLNDMDVMEAAMLTASQKGYLNATDCADYLVEKGIPFRDAYQITGNIL
                     KECEEQNLTLENFPLEMYKKHSDAFDETIYKKVDLKECVIRRNVAGGPAPERVKEHIE
                     AVKTKLEDYQIIS"
     gene            complement(3199..4464)
                     /locus_tag="PLT73_01995"
     CDS             complement(3199..4464)
                     /locus_tag="PLT73_01995"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRM19184.1"
                     /translation="MFKANKHRIENHIESLARFTSTPGHGTTRLSYSKEDAAARHYIK
                     QEMNNLGLVVREDAVGNIYGRMEGALKNAPAVIIGSHFDSVPNGGSFDGPAGIVTGLE
                     VVALFRDYGFEPQYPLEVIAMIEEEGSRFGGGVLGSRMIAGQITLVDLNEMKDNTGLS
                     AAEAMEKMGFNAAEIGSAQRTGKDVKAFLELHIEQGPLLEAAAQDVGIVEMIVGMTEI
                     RITVKGKSGHAGTTPMEGRADALAGAVSILKELPGMVLDEPDRPVLTVGKLNVLPNGA
                     NVIPNQVTFTVDIRSAHSDTIQRILQKIEQLVADVKIPGISFSTEELLYAEPVEMSAD
                     IQTLLIENCEKLGLRYRKMVSGAGHDAMIFADFTETGLIFVPSKDGISHTPEEWTDYA
                     QLQKGIEVAFETIVQLTEAKEKSSNTETY"
     gene            4891..5640
                     /locus_tag="PLT73_02000"
     CDS             4891..5640
                     /locus_tag="PLT73_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRM19185.1"
                     /translation="MKPIIGIGGNHLTTELGNQMEKTYTPQGFIDGVQVAGGIPLVIP
                     ISDPMDAEHYINAVDGLILAGGQDVSPILYDEEPTHRLGVTHPARDAFEIALAREAYN
                     QCKPIFAVCRGMQLINVAFGGSLHQDISQLEKYDVQHDQKTNIQYASHSISIAPNNFL
                     RSFLGEKHLVNSFHHQGVKELAEPFEAIAWSSDGLIEAFQAKERSQNIFAVQWHPEFM
                     LASSQKMQYFFDEIVFLAGTGIHYQDNVMHY"
     gene            5787..>6222
                     /locus_tag="PLT73_02005"
     CDS             5787..>6222
                     /locus_tag="PLT73_02005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRM19186.1"
                     /translation="MKRVPNILTLIRLFLIPLYLTVFYSDTAHPVRWAMIIFLVASLT
                     DIIDGYIARKYDAITKFGQVADPFADKVMQLSVLYTLMDIGYIENWFFLIVLIKDGLQ
                     IFLGVALLNVEPKIIVPANVFGKATTVLIFATILLSLFRLPGL"
BASE COUNT         1627 a         1422 c         1534 g         1639 t
ORIGIN      
        1 gcgagattat ccaaaccgag ccgaatatct gaatatcaag gaaaacgtga ggctttttgg
       61 catcacgttt tttgcattcc atagcgtcat aatttctaaa tacaggcatt tgttctataa
      121 tgaagatagg taaatgtcag gttatgaaga gggtcaaatc agaaatgagg ggatcatagt
      181 gaaaaaaacg aagatagtct gcacaatcgg accagccagc gattccgtcg atgtgctggt
      241 aaggatgatg gacagcggca tgaatgtagc acgtttgaat ttttcgcacg gtgcacacga
      301 tacgcatatg atcagcctga ataatgtcag gggagctgca cgattggcga acaaaaatat
      361 aggtatcatg ctggatatcc aaggccagaa aatccgcaca aataagatga ccgatgacgc
      421 cgttaccttg gtttccgggg agtcggtaac catcagcatg aaacctgtgc tgggaacaaa
      481 agaaaaattt tctgtcacct atcctgagct gatcaatgat gtgacgatcg gcatgcatat
      541 tttgatcgat gatggcctgt tggttctgga ggttacggac atcgattatg aagctaaaga
      601 gatcatagcg attatccaag tgggcggtac cctgaagaat tcaaaaggta tcaatgtgcc
      661 ggaagccaag ttcagtatgc cggggctgac ggaaaaagat gctgcggata tccgcttcgg
      721 tgttcagcaa ggagtggact tcatcgctgc aagctttgtc cgaagacctg atgatatcct
      781 ggaaattatc gaaatccttg aagaaatgga tgcgacggac acgcagatca tcgccaaaat
      841 cgaaaaccag gaaggcgtcc agaatgccga agagatactc aaggtgtgca gcggcctgat
      901 ggtggcgcgc ggcgatttgg gtgtggaaat caacggaggc gacgttccaa tcgtacagaa
      961 agacctgatc cggaaatgca atctggcagg aaaacaggtc atcaccgcca cgcagatgtt
     1021 ggactccatg caatggaatc cgcgtccgac aagggcagaa gcgagcgacg tcgcgaacgc
     1081 catatttgac ggaacagacg cagtcatgct ttccggcgaa tctgctgtcg gccagtaccc
     1141 agtcgaaact gtcgcgatga tggcgcgtat cgcggaacgg acggaagaag ctcttgaggc
     1201 aaatggcggc aacctgtccg ccttgtctat gttcgacaag gcctccgtta ccgaagcgat
     1261 cagccaagct gcagcccgca cggtcatgaa tttgggcatc aagaccatcg tagcggcaac
     1321 cagctccggg catacggccc ggatgatctc aaaataccgc ccgaaagcga atattttggc
     1381 tgcaacctac agcgaaaggg tgcagagagg gctgacgctt gtctggggtg tgcaagcttt
     1441 cgtcattgca gagcctgaaa atgttgacga tatgctaaat aatgtaaccg cattggcgaa
     1501 agaaaagggc ttcgcgcaac ctggagacgc catcgtcatc actgctggga cgccgataag
     1561 agaaagcggc acgaccaatt tgatgaaaat cgaacgcata cctgattgaa cgcaaaacac
     1621 tgagcagatc ggttttttgt attgggattg gatttatact aagagattcc accgccaaac
     1681 cacagaaaga ggcagcctcg aaatgaggct gcctctttct gtcattgatt gatcaggaga
     1741 tgatctggta gtcttctaat tttgtcttga ctgcttcgat atgctctttc acgcgttccg
     1801 gtgctggtcc gccggctaca tttctgcgga tgacgcattc cttcaggtcg accttcttat
     1861 agatggtttc gtcaaaagca tccgagtgtt ttttgtacat ctccaacggg aagttttcca
     1921 gcgtaaggtt ctgttcttca cactctttca ggatgtttcc ggtgatctgg taagcgtcac
     1981 ggaaagggat acctttttcg acaaggtaat cggcgcagtc ggtggcattc agatagcctt
     2041 tttgcgaagc cgtcagcatg gctgcttcca tcacgtccat atcgttcaac attgcaggca
     2101 ggatttccaa acagaaggtc actgtgtcga tcgcttcgaa cagcaattct ttttcttcct
     2161 gcatgtcttt gttgtatgcg agcggcaaac ctttcatcaa agtcaatacg cccatcaaat
     2221 cgccgaagac acggccggat tttccacgca acaattccgc gatgtccgga tttttcttct
     2281 gcggcatgat gctggagccg gaagagaacg tatcccggat tttcacaaaa ccgaactcct
     2341 gtgatgacca gatgatgact tcttcagcca gacgggaaat gtggaccatg atgatcgaca
     2401 gatccgccaa catttctaag gaatggtcgc gatcggatac gccatccaaa ctattccggg
     2461 aaaaattctt gaatccgagt gcttcagcag tgaaggcgcg gtccaagggc aaggtagtag
     2521 tcgcaagcgc ggcactgccc agcggcatat aatgcagtac ccgttctttg gcatcatcca
     2581 gacgggactt gtccctcagg aacatctccg tgtaagccat caggtgatgg gcaaaagtga
     2641 tcggttgtgc acgttgcaga tgggtgtaac ctggcatgat cgtttccaga tgtttttcgg
     2701 ctttttcgct caatgcgagg gcaacgttgg cagtgaggct tgacagatgc tccaacgcag
     2761 atacagaata caatttcaaa gcagtcgcta cttgatcgtt acggcttctt cctgtgtgga
     2821 cctttttgcc gatagcgcca aggcgacgcg tcagttctgc ttccaggaag ctgtggatat
     2881 cttcttccga gaaatcgatg gtcagatcgc catcctgcag ttcatcattc attttgccca
     2941 gttcattgac gagggcatct gcttcttcgc ggggaatgat ttcggttttg cccaacattt
     3001 ttgcgtgagc gatacttccg gcgatatcgg catagaccag acgggcatcc gcatcgatgg
     3061 atgaattgaa ttcaaatgcc ttttcattca tggaaccgtg ataattattt gaccaaattg
     3121 ccataatttc ctcctcggca gggtgcttaa ctttaggtgg atcaatacac tgcaatccct
     3181 ggctgtttta atagtgtttt agtacgtttc tgtgttgctg cttttttctt ttgcttccgt
     3241 caattgtaca attgtttcga acgcaacttc aatccctttt tgcaattgcg cataatccgt
     3301 ccattcttcc ggtgtatggc tgatgccatc tttgctggga acgaaaatca agcctgtttc
     3361 agtgaaatcc gcgaatatca tggcgtcatg accggcaccg ctgaccattt tccggtaacg
     3421 cagacccagt ttttcgcaat tttcgatcag caaggtttgg atatccgctg acatctcgac
     3481 cggctccgca tagagtaact cctcggtact gaaagagatg ccgggtattt tcacatcagc
     3541 cacaagctgc tcgattttct gcaggatgcg ctggatagta tcgctatggg cggaacggat
     3601 atccaccgta aaagtaacct gattcgggat gacattggct ccgtttggga ggacgttcag
     3661 ttttccgaca gtcaaaaccg gcctatcagg ttcatccaat accattcccg gcagttcctt
     3721 caatatcgat accgcccccg cgagggcatc agcacgacct tccataggag tcgttcccgc
     3781 atggccggat ttccctttca ctgtgatgcg aatttcggtc ataccgacaa tcatctctac
     3841 gatacccaca tcctgagctg ctgcttccaa taaaggaccc tgttcgatat gcaattccag
     3901 aaaggctttt acatcctttc cggtgcgctg agccgagccg atttcagcag cgttgaaacc
     3961 cattttttcc attgcttcag ctgcggaaag gccggtattg tccttcattt cgttcaaatc
     4021 tacaagagtg atttggccgg ctatcatgcg ggaacccagc acgccgcctc cgaagcggga
     4081 accttcttct tcgatcatgg caatgacttc gagcgggtat tgaggttcga agccataatc
     4141 cctgaacaaa gcgactactt cgagtccggt gacgatgcca gcagggccat cgaaagagcc
     4201 tccattcggg acactgtcaa aatgtgaacc gatgatgacc gcaggcgcat ttttcaaggc
     4261 accttccatt cttccataaa tgtttccgac ggcatcttcc cgtacaacca ggccaaggtt
     4321 attcatttct tgtttgatgt aatggcgggc tgctgcatct tccttactat aggaaagtct
     4381 ggtcgttccg tgcccaggag ttgaggtaaa acgtgcaagt gactcgatgt ggttctcgat
     4441 gcggtgttta tttgccttaa acataattat ttgtaggtct cccttcttaa tgctgcctat
     4501 ttataaggct acatgcaaat gcggacattc tcattttttt ttaatttcat tttctatatt
     4561 gaatcctaga gcaaaaataa ccaaaaagca agataaatca aggtttatca taataaacgg
     4621 agcataaaaa tgaacagggc tactgaaaac tgccgcaaat cgcgattgac agagagacaa
     4681 cagcacgtta caatatcatt aatttatttt taaatgtgaa tttgatgaaa aaagtattgg
     4741 aaagtaatga aataagcagt gggatgagaa gaatctacag ctgtatgatc atatagatat
     4801 atgttgcggg aaaggaaagc ttgtacctgg aaagtgtccg tgaagcatgg ccacacttca
     4861 ttagaaaaaa tgtgacggga ggaataatgg gtgaaaccga ttataggaat tggaggcaac
     4921 catctgacta ctgaactcgg aaatcagatg gaaaaaacat atacaccgca aggatttatt
     4981 gatggtgtac aagtagcagg aggaattccg cttgtgattc cgatcagcga tccaatggat
     5041 gcagagcact atataaacgc cgtggatgga ttgattttgg cgggcggtca ggacgtatct
     5101 cctattcttt atgatgagga accgacacat cgcttgggtg tcacgcatcc ggcaagggat
     5161 gcttttgaga ttgcattggc cagagaggca tacaatcaat gcaaaccgat ttttgcggta
     5221 tgccgcggca tgcagttgat caatgttgct tttggtggca gcctgcatca ggatatcagc
     5281 cagcttgaaa agtatgatgt ccaacatgac caaaaaacga acatccagta tgcttcccat
     5341 tcgatttcga ttgccccgaa taatttcttg cgttcctttt taggggagaa acacctcgtc
     5401 aactctttcc atcatcaagg cgtcaaagaa ttggcggagc cttttgaggc catcgcttgg
     5461 agctccgacg ggttgattga ggctttccag gcaaaagaaa gaagccagaa tatcttcgcg
     5521 gtgcagtggc atccggagtt tatgctggcc tcttcccaga aaatgcaata tttctttgat
     5581 gagatcgtct tcctggcggg aacaggcatc cattatcaag acaacgtgat gcattattaa
     5641 gtattgttca aaaccctgca gctatccggt tgctgggttt ttgaactttt gtgtgggttc
     5701 gaacctgcta aaaaatagct aaagcacccg ctatagatta gaatataggt aagaagaatg
     5761 ccaatggtag ctgggaggca aatgatatga aacgtgtacc gaacatcttg acgctgattc
     5821 gcctattttt gattccactt tatttgacgg tcttctattc cgatacggcg catccggtcc
     5881 ggtgggcgat gattattttt ctggtcgcca gccttaccga catcatcgat ggctatatcg
     5941 ccaggaaata tgatgccatc acgaaattcg gacaggtggc tgacccgttt gcggataaag
     6001 tgatgcagct ttcggttctg tacactttga tggacatcgg gtatatcgaa aactggtttt
     6061 tcctgatcgt attgataaaa gatggcctgc aaatattcct gggcgtggct ttgctgaatg
     6121 tcgagccgaa gatcatcgtg ccggcgaatg tgttcggaaa agccactacc gttctgattt
     6181 ttgcaacaat cttgctttct ttgttccgcc tgccgggatt ga
//
LOCUS       DAOMUJ010000044         6217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3400_length_6217_cov_2.425511, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000044 DAOMUJ010000000
VERSION     DAOMUJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_6217_cov_2.425511"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..549
                     /locus_tag="PLT73_02010"
     CDS             211..549
                     /locus_tag="PLT73_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM19187.1"
                     /translation="MLTKEELPFCPVATTVDLIGNKWKLLIMRELLTGTKRFNEMHRL
                     VDGISQKVLTENLRKMESDGIVKREVFPEVPPRVEYSLTDLGDSLRPIINSMSDWGTD
                     YIKNNLLEQK"
     gene            989..1279
                     /locus_tag="PLT73_02015"
     CDS             989..1279
                     /locus_tag="PLT73_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19188.1"
                     /translation="MIDPLLVFAATFDTDIKIAIMYLFLCYPTMSCNDIVLMTGEKKE
                     SVVTSLWRLQMDTIVVNKIEDDRNCYYSLTSSGTASLKVFSEMADFSDRCLK"
     gene            1293..1922
                     /locus_tag="PLT73_02020"
     CDS             1293..1922
                     /locus_tag="PLT73_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19189.1"
                     /translation="MLIDDILQYFQENFSQYLIYVYQHLGLSIQAIGISCLIGIPLGY
                     ISHKHRKLSQMITLSSQALRIIPSLAVLFLLISLIGVGRAPALIALVLLGIPPILVNT
                     TVGFLEVPAVMIETGKGLGMTDRELLKRVKIPMAFPFVMTGVKLALVEIISSATLATY
                     IGAGGLGTLIFTGLGLNRMDLLLIGGVSVAMIAFTTSIFLDYIIKRSIN"
     gene            1923..2831
                     /locus_tag="PLT73_02025"
     CDS             1923..2831
                     /locus_tag="PLT73_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM19190.1"
                     /translation="MKRKNIISTIAMLAVTLMVSGCSGLTGGSDSSTEDTAIRVGSKD
                     FTENLVVSEIYALALEDAGYEVERIPNIASSVVHTSITNDEIDLYPEYTGTGLLVVLE
                     MEIETDPQKVYDTIKAEYDEQFDLTWLDYAQANDSAGLVIKTSVAEKYGIETISDLQE
                     NASELRFASQGEFDLREDGLPGLTKAYGEFNWKSSTVYDNSLKYEVLKNDEADVAPAY
                     TTEGQLTNKEEFTVLVDDKKFWPPYNLAPVIRNEVLEVNPDIAEILNNISSTLDTETM
                     TNLNAKVDVDGQEYEAVAKEYFDSIK"
     gene            2854..3627
                     /locus_tag="PLT73_02030"
     CDS             2854..3627
                     /locus_tag="PLT73_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006418298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19191.1"
                     /translation="MEQTAIEFKNVTKLYGEGETKAVDDISFEIKEGEFITVLGSSGS
                     GKTTTLKMINRLIREDAGNITFFGEDIGGMNEVDLRRKIGYVVQQIGLFPHMTVAQNI
                     ATVPELLKWDKKEIQARVKELLELVQLDPNVFADRKPKQLSGGQQQRIGVARALAANP
                     KVMLLDEPFGAVDAITRLQLQNELQKIHKELGDKTFVLVTHDINEAFKLGTRVLVMDK
                     GKICQFDAPREIMRHPKTDFVADLIATVKEQEAFWSELK"
     gene            3624..4247
                     /locus_tag="PLT73_02035"
     CDS             3624..4247
                     /locus_tag="PLT73_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19192.1"
                     /translation="MITYWNTYHEKLLTATMQHIELVGTTLMIAILIAGGVILMCMHN
                     ERVMNGLIYLTSLLYSIPSLALFAILIPLTGLGRNTAIIVLVIYCQYVLLRSFSAGIK
                     EIDSTIIEAAVGMGMTRNQMFRKIQLPLAMSSIIAGIRIAATSTIGIATIAATINAGG
                     LGTVLFDGLRTFSVVKLLWGTVLSMLLCLLVNVILYLLENALRRRFA"
     gene            4269..5144
                     /locus_tag="PLT73_02040"
     CDS             4269..5144
                     /locus_tag="PLT73_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HRM19193.1"
                     /translation="MSDIWNPWHGCKKVSPGCQNCYMFHLDSQRGKDGALIYKVQSSF
                     DRPLKRNRRGDYIIPPGSTIRVCMTSDFFLEEADEWRGEVWKMIEKRSDVLFYLLTKR
                     IERVKDCLPENWGDGWENVWMNVSAENQKMADRRIPVLLDLPFKHRGIMAAPLIGEID
                     LTKHLSSGKIERVIVGGENYDGARECHYKWVQRMHQDCVDHHVNFEFIETGNHFVKNG
                     RKYEILDKRKQQEQAKKSGLFYEGRAVSVHLTEVAQGESVPLFRTNAVTLCDSCAYKK
                     FCGTQSGRPSCMLSL"
     gene            5169..6152
                     /locus_tag="PLT73_02045"
     CDS             5169..6152
                     /locus_tag="PLT73_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM19194.1"
                     /translation="MKAVVVHEAGGPEKLVHQEVPLPEIREGWSLVKVMGFGINHSEI
                     FTREGKSPSVKFPRILGIECVGIVEQTNDEEKLPLGQKVVSIMGEMGRAFDGSYAEYV
                     LLPNDQIYPIHTDLPWAQLAAIPETYYTAFGSMKNLRIQASDQILVRGATSGVGVAFA
                     KLVKAQFPDIRIYGSTRKAEKGERLKEAGFTDYVEDRNGVLETEETFDKILELIGPAT
                     IKDSLSHIREGGVVCSTGQLGGKWFLEDFDPIMELKHNSYLTTFHSSDVSGEKVNELL
                     EYIEEKGIAVDPERVFPLEEVAQAHAFLQSQDSFGKAIVLDPSAAGEKASE"
BASE COUNT         1743 a         1285 c         1670 g         1519 t
ORIGIN      
        1 caaaatccag tgcccgcgag ggcgtgccgg ttcgaccccg gccgccggta tcacacaaaa
       61 caggctcctg acgatgacat catcgttgga agcctgtttt tttgcatagt tacacccgaa
      121 actgaaagga atatttgaaa ttgtttcatt tcaagtatat aattcgttgt aacttacaaa
      181 aagaaactta ctatctatga ggtggaaaag atgctgacca aagaagaatt gccattctgc
      241 ccggttgcga caaccgtcga tctgatcgga aataaatgga aactgctgat aatgcgcgaa
      301 ttattgacgg gaaccaagcg ttttaatgaa atgcaccgat tggttgatgg catcagccaa
      361 aaagtattga ctgaaaatct ccggaaaatg gagtctgatg ggattgttaa acgggaagta
      421 tttcctgaag tgccaccccg cgtggaatat tcattaaccg atcttggtga ctccctaaga
      481 ccgatcatca acagcatgag tgattgggga acggattata tcaaaaataa tctcttggaa
      541 caaaaataag ccagcatagg aagtaggaaa gcgggttgca aatccgacct tttctgtgat
      601 cccatacggg tgccaaaagt tgtatcattt tcatactccc ttttgaggcg tcataatgct
      661 tcaaaaggga ttttttttta caattttttg ttgtaggtat cattgtagct gtagcttaca
      721 aaaaggtaag tacctgataa aaaagtgcgt acttttgtaa gggcatcttt aaagatatag
      781 taagagtatg gaaatccatc cataagtatg gaaatccatc cataagtatg gaaatccatc
      841 cataagtatg gaaagggaga aacatcattg aggtgttaag caagtcagac acgcacgaat
      901 gcccatcatg atgatattgc ttgtgcgaag aagctggctc agcggtaatt ttcggtgcgc
      961 gtgtgttgaa ggaggcggaa tgcaggtcat gatagatcct ttattagtat ttgcggcaac
     1021 ctttgacact gatatcaaga ttgccataat gtacctgttc ctttgctatc cgacaatgag
     1081 ctgcaatgat atcgtcctaa tgacgggtga aaaaaaagaa agtgtggtca ccagtctgtg
     1141 gagactgcaa atggatacga ttgtggtcaa taagattgag gacgacagaa actgctatta
     1201 ttccttgacg agcagcggta cagcgagttt gaaggtattt agcgagatgg cagatttttc
     1261 ggatcgttgt ttaaaatgag aggaggtccg aaatgctaat tgatgacata ctgcaatact
     1321 ttcaagagaa tttcagtcaa tatttgattt acgtttacca acaccttgga ttgagtatcc
     1381 aggcaatcgg aatttcttgt ctgatcggta ttccgctggg atacatcagc cacaagcacc
     1441 ggaaactcag ccagatgatc accctcagtt cccaagcctt gcggatcatc cccagtttgg
     1501 cggtcctctt tttattgatt tccttgatag gcgtcggcag ggctcccgca ttgattgctt
     1561 tagtgttatt aggaattccg ccgattttgg tcaacacaac ggtcggattc ctggaagtcc
     1621 ccgctgtgat gatcgaaacg gggaagggtt tgggcatgac cgatagggag ctcctgaaaa
     1681 gggtgaagat tccgatggct tttccttttg tgatgaccgg cgtgaaactg gcgttggtgg
     1741 aaattatctc gagtgcgacg ctggctacat acatcggtgc gggtggtttg gggacactga
     1801 tttttacagg attgggtttg aacaggatgg atttgcttct cattggagga gtatcggtag
     1861 cgatgattgc cttcacgaca agcatctttc ttgattacat aattaaaagg agcatcaatt
     1921 aaatgaaaag aaaaaatatc atcagtacga ttgcaatgtt ggccgtaaca cttatggtct
     1981 caggttgttc aggcctgact ggcggaagcg atagcagcac ggaagataca gccatccgcg
     2041 tgggttcaaa ggacttcacc gaaaacttgg ttgtttcgga aatttatgcg ttggctttgg
     2101 aagatgccgg ctatgaggtt gaaagaattc cgaatattgc cagctcggtt gttcacacat
     2161 cgatcaccaa tgatgaaatc gacctttatc cggagtatac agggacaggc ctgttggtag
     2221 tactggaaat ggagatagaa accgatccgc agaaagtcta tgacacaatc aaagcagaat
     2281 acgatgagca gttcgatctg acctggttgg attatgcgca agccaacgac agcgctggtc
     2341 tggtcatcaa aacgagcgta gccgaaaaat acggaattga gaccatttcc gatctgcagg
     2401 aaaatgcttc agagctgcgt tttgcatccc aaggtgaatt cgatctgagg gaagatggcc
     2461 ttcctggttt gaccaaagca tacggagaat tcaattggaa gagttcgaca gtctacgaca
     2521 acagtttgaa atatgaagtc ttgaaaaatg atgaagcgga tgtcgctccg gcctatacga
     2581 cagaaggaca actgacaaat aaagaagaat ttaccgtctt ggtggatgat aaaaaattct
     2641 ggccgcctta taacttggct ccagtaatcc gaaatgaagt gttggaagtg aatcctgaca
     2701 ttgcggaaat tctgaacaac atcagcagta ctttggatac ggagactatg acgaatctga
     2761 atgccaaagt ggatgtcgat ggacaggaat atgaagcggt agcgaaagag tactttgatt
     2821 cgataaaata aactaagggg gcgttgaagc cgcatggagc aaaccgcgat agaattcaaa
     2881 aatgtgacga agctctacgg ggaaggggag acgaaggcag tcgatgatat ctcttttgag
     2941 attaaggaag gcgagttcat cactgtcctg gggtcttctg gctccggcaa aacgaccact
     3001 ttaaaaatga tcaaccgctt gatccgtgaa gatgcaggaa acataacctt cttcggggaa
     3061 gacatcggcg gaatgaacga agtggacctc aggcggaaaa tcggttatgt cgtccaacaa
     3121 atcggtctgt ttccgcacat gaccgttgcc caaaacatcg cgacagtccc ggaactgctg
     3181 aagtgggaca agaaggaaat ccaagcccgc gtcaaagaat tgctggagct tgtccaactg
     3241 gatccgaatg tgtttgccga cagaaagccg aaacaattat ccggcggtca gcaacagcgt
     3301 atcggcgttg cgagggcgct tgccgccaat ccgaaagtga tgctgctgga tgaaccattt
     3361 ggagcggtcg atgcgatcac gcgcctacaa ctgcagaatg aactgcaaaa gatccacaaa
     3421 gaattgggag acaaaacctt tgtgctggtc acccacgaca tcaatgaggc gttcaagctg
     3481 gggacgagag tgctggtaat ggacaaaggc aagatttgtc aatttgatgc accgagggaa
     3541 atcatgcgcc atccgaaaac cgattttgtt gcggatttga ttgccaccgt aaaggaacaa
     3601 gaggcatttt ggagtgagct gaaatgatta catactggaa tacttaccat gaaaaactgc
     3661 tgaccgccac gatgcagcac atcgaattag tggggacaac cctgatgatc gcgatcctca
     3721 tcgcaggcgg agtaattctg atgtgcatgc acaatgaacg tgtcatgaat ggtttgatct
     3781 atctgacatc tttgctttat tcgattccga gtcttgctct gtttgccatc ttgattccgt
     3841 tgacgggatt gggaaggaac acggccatca tcgtgttggt gatttattgc caatatgtcc
     3901 ttttgcgcag tttttccgca gggatcaagg aaatcgattc gacaatcatc gaagccgctg
     3961 tcgggatggg catgacgagg aatcaaatgt tcaggaaaat ccagcttccg ttggcaatgt
     4021 catccatcat tgcagggata cgaatcgccg cgacctcgac aatcggcatc gcaaccatcg
     4081 ccgcaacgat caatgcaggc gggttgggaa ctgtcctttt cgatggcttg cggacattca
     4141 gcgtcgtaaa actgttgtgg ggcactgtgc tgtcgatgtt gttgtgtctg cttgtcaatg
     4201 tcatcctgta cttacttgag aacgcgttgc gacggagatt tgcctgatct tttcaaagga
     4261 gtggtcgctt gagtgatatc tggaacccgt ggcacggctg caaaaaggtc agcccgggat
     4321 gccaaaattg ttatatgttt catttggaca gtcaacgagg aaaagatggc gcgctgattt
     4381 ataaagtcca gagcagcttc gatcgcccgc tgaaaaggaa ccggcgcggc gactacatca
     4441 taccgcccgg ttccacgata cgcgtctgca tgacttcgga cttttttctg gaggaagcgg
     4501 acgagtggcg cggggaagtc tggaagatga tcgagaagcg gtcggatgtg ctgttctatt
     4561 tgttgacgaa aagaatcgaa cgcgtgaaag attgcctgcc agaaaattgg ggagacggtt
     4621 gggaaaacgt gtggatgaat gtctctgccg aaaaccaaaa gatggccgat aggcggatcc
     4681 cggtactcct ggatctgcct ttcaaacacc gaggcatcat ggccgcaccg ttgatcggcg
     4741 aaatcgactt gacgaagcat ttgtcgtccg gaaaaatcga acgcgtcatc gtcggcgggg
     4801 aaaactatga cggagcaaga gaatgccatt ataaatgggt gcagaggatg caccaagatt
     4861 gcgtggacca tcatgtgaac ttcgaattca tcgaaaccgg gaatcatttt gtgaagaacg
     4921 gacggaagta tgaaattttg gacaaaagaa agcagcagga gcaggcaaag aaatccggct
     4981 tgttttatga agggcgagcg gtttcggttc acctgactga agttgcccag ggcgaatcgg
     5041 tgccgctttt tcgaaccaat gccgtgactt tgtgcgattc ctgtgcatac aagaaatttt
     5101 gcggtacgca atcgggaaga ccaagttgca tgttatcatt atgaaaaatt aagacagggg
     5161 gtggagaaat gaaggcagtc gtagtgcacg aagcgggggg accggaaaaa ctggtgcatc
     5221 aggaagttcc tcttccggaa atccgggaag gctggtcgct cgtgaaagtc atgggcttcg
     5281 gcatcaacca ttcggaaatc tttacacggg aggggaaatc cccttcggtc aagtttcccc
     5341 gcattttagg catcgaatgt gtggggatag tcgaacagac gaacgacgaa gagaaattgc
     5401 ctctcggtca aaaagtggtg tcgatcatgg gagagatggg gagggccttc gacgggagtt
     5461 atgcggaata tgtcctgttg ccgaatgacc aaatctaccc catccacacc gatttgccct
     5521 gggcccaact ggcggccatt ccggaaacct actatacggc tttcggttcc atgaagaatc
     5581 tgaggatcca agcatccgat caaattttgg ttcggggggc aacgagcgga gtaggggttg
     5641 cctttgccaa gttggtcaaa gcccaattcc cggatatcag gatttatggg agcacgcgga
     5701 aagccgaaaa gggtgagaga ttgaaagaag ccggcttcac cgactacgtc gaagaccgca
     5761 acggcgtgtt ggaaacggaa gagactttcg acaaaattct ggaattgatc ggccctgcga
     5821 cgatcaagga cagcctttcc catatccggg aaggcggcgt tgtctgcagc accggtcaac
     5881 tgggcgggaa atggttcctg gaggatttcg atcccatcat ggaattgaag cacaacagct
     5941 acttgactac tttccattcg agtgacgtct ccggcgagaa ggtgaatgaa ctgctggagt
     6001 acattgaaga aaaaggcatt gctgtggatc ccgagcgagt cttcccgctc gaagaggttg
     6061 cccaagccca tgcctttttg cagagccagg acagtttcgg aaaagccatt gtgttggatc
     6121 cgagcgctgc gggagagaaa gcttcagaat agggttgcgt tttcgcggat tattggttag
     6181 aatcaaatga gaaacactgg gggtgcgcta ttgcgct
//
LOCUS       DAOMUJ010000045         6214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3403_length_6214_cov_2.512421, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000045 DAOMUJ010000000
VERSION     DAOMUJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_6214_cov_2.512421"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLT73_02050"
     CDS             complement(<1..327)
                     /locus_tag="PLT73_02050"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRM19195.1"
                     /translation="MEMSFRWYGHEDPVTLKNIRQIPGMKGIVTAVYDIPVGQPWPLE
                     RIEKLKADVEAEGLYISVIESVPVHESIKLGRPDRDQYISAYKETLTNLGKAGIPVVC
                     YNFMPIF"
     gene            complement(360..1775)
                     /gene="uxaC"
                     /locus_tag="PLT73_02055"
     CDS             complement(360..1775)
                     /gene="uxaC"
                     /locus_tag="PLT73_02055"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRM19196.1"
                     /translation="MSFIHDDFMLQSETAKKLYHEYAENMPIFDYHCHLVPQQIAEDY
                     EFENITELWLGGDHYKWRAMRAMGIPEEKITGSASPEEKFEAWAYTAENAVGNPLFHW
                     TALELKKYFHIEETLTSANWKEIYAECNRVLKAEKLTARQLIKNSNVTFICTTDNPTD
                     TLEWHKAIAEDETFDVAVVPGFRPDEAFAIQDAAKFAGFIGKMQEATGKEMTTFAELL
                     EGMEERIQYFADHGSNVSDHGLSQIYYAEATDEEIEAIYAKAIAGDSISEAEYAKYQT
                     RLLVELGKIYAAKDFVMQLHFGAIRNNNKRMFAKLGADAGFDSIQDQPNVSYALNNLL
                     GAMDLTNELPKFIAYNLDPTYFDLVGTAITNFQANDKGIKSKVQMGSGWWFNDTKYGM
                     LKQLKSLSEAGLLMNFVGMLTDSRSFISYTRHEYFRRILCDFIGDLVERGEIPNDVKL
                     LEKLITNISYNNAVEYFDFKK"
     gene            complement(1818..2465)
                     /locus_tag="PLT73_02060"
     CDS             complement(1818..2465)
                     /locus_tag="PLT73_02060"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HRM19197.1"
                     /translation="MSVKRDTLAQLQENYLFAVVRGASEEAGYEISKAAYQGGIKNIE
                     VTFSTPGAENVMRKLSDEFAGTDMVVGAGTVLDEVAARISIMNGAKFIVSPSFNEKIS
                     RMCNLYTVPYLPGCGSVTEIQMALETGCEVVKLFPGGLLGPGFIKDVHGPIPWVDVMP
                     SGGVSIENMDKWIANGAWSVGVGSALTRNLKEGGYASVTAAAKEFADRLAEIKAQ"
     gene            complement(2721..3494)
                     /locus_tag="PLT73_02065"
     CDS             complement(2721..3494)
                     /locus_tag="PLT73_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM19198.1"
                     /translation="MVQKEAVNTSPKVQSIDRALAILETLADYPSLSLMELSEKVHLH
                     KATTHRLVNSLMENGYIDRNPDTKQYRISLKMFHLGNKRVHNIDFLNVAKSMIRQLSD
                     DTRQTVHLVVEDNDEVLYIDKYGESRGVRMQSKIGTKAPLYCTAVGKALLSTRQNAAV
                     REYWDSIVPEQKTGRTITTYEDLISELDEIRRNGYALDDEEFEEGIVCIAAAFSSARE
                     VAAGAISISLPLSDMVDKEFYTGKILEYSTKISQLLGHF"
     gene            3943..>4504
                     /locus_tag="PLT73_02070"
     CDS             3943..>4504
                     /locus_tag="PLT73_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM19199.1"
                     /translation="MAKNHIIHTAKRPFGFRDILGYFMGDFGCNMSFTLISSYMFIFY
                     TQYIGISLAHYSIIILITKIFDGINDPIIGAIVDRMGADKKGEKFKQWILRGSPFLAL
                     AATVIFIDAADWSYAAKLGLCIGGYILWDLTYTVVNVPYGALSSVMTSKPSQRAALSN
                     ARSWGYIIAGVPLGILIPLFAYEQVTK"
     assembly_gap    4505..4523
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4524..5363
                     /locus_tag="PLT73_02075"
     CDS             <4524..5363
                     /locus_tag="PLT73_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM19200.1"
                     /translation="FRGERMFPIAIILGIISIVAFALLYLNTEERIQHTESEGEKVKF
                     NYFITLKDFFRNRAILGISLASIAQILFIMGGNQLHQLTYQMYFRDGTLSSYAILTQI
                     IPLFVGTALGGSLVGKLGKKELSTWPLLGAIAVYAFLYLVEIKNPILWITMQIIANSF
                     AFGLAIYTWGMVADAIDYQEWKTGIRNEGSVYATYSMLRKIAQGFGQSFIPAAIAILI
                     PTLDVKDAFTWTSENALALRDMAVLFPLIGYIIIFICFKFIFNLDRKTVAEMQVALGR
                     DEE"
     gene            complement(5434..>6214)
                     /locus_tag="PLT73_02080"
     CDS             complement(5434..>6214)
                     /locus_tag="PLT73_02080"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM19201.1"
                     /translation="APSSVYTMHDVHEAGGVPAIMNELIKHGNLHPDRITVTSKTIRE
                     NVAGHEIKNEEVIHKYENAYSPVGGLSILNGNIAPFGGVIKVGGVDADIKKFVGKAIC
                     FSSHDEAVEAIDNRTVKKGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGRDVALIT
                     DGRFSGATRGIAIGHISPEAASGGPIGLIEDGDEIVIDLEHRTINVSVADEVLEERKK
                     KQTRFVSKVKTGWLARYAALVTSANTGGVLKTPEEIFAEYE"
BASE COUNT         1586 a         1451 c         1276 g         1882 t
ORIGIN      
        1 aaagatcggc atgaaattgt agcatacaac cggaattcct gctttaccca agttagttaa
       61 ggtttctttg taggcactga tgtattgatc acgatctgga cgacccaatt tgatggattc
      121 gtgaaccgga acagattcaa tgacagaaat gtatagacct tctgcttcta cgtccgcttt
      181 caatttttcg attctttcca atggccacgg ttgaccaacc gggatatcgt agactgcagt
      241 aacgattcct ttcatgccag gaatttgacg gatatttttc aaagtaacag ggtcctcgtg
      301 accgtaccat ctgaatgaca tttccatttc aaaaacactt cctcttcttt ttttgttttt
      361 tattttttaa aatcaaagta ttctacagca ttgttgtagc tgatgttcgt gatcagtttt
      421 tccaatagct tgacatcatt cgggatttct ccacgttcca cgagatcacc gatgaaatca
      481 caaaggatgc ggcggaaata ctcatgacgc gtataggaga tgaaacttct tgaatctgtc
      541 agcatgccga caaaattcat caacagacct gcttcagata aggatttcaa ttgtttcagc
      601 atgccgtact tggtgtcgtt gaaccaccaa ccggaaccca tctgaacttt gctcttgatg
      661 cctttatcat tcgcttggaa gttcgtgatt gccgttccca ccaagtcgaa ataagtaggg
      721 tcaaggttat aagcgatgaa tttaggcaat tcattcgtca agtccattgc accaagcaga
      781 ttgttcaaag cataggatac gttcggttga tcctggatgg aatcgaagcc ggcatccgcg
      841 cctaatttag caaacatgcg tttgttgttg ttgcggatag cgccgaagtg caattgcatc
      901 acgaagtctt tggccgcata gattttgccc agttccacca acaagcgtgt ttggtatttt
      961 gcgtattccg cttcagaaat gctgtcacca gcgatcgctt tggcgtagat ggcttcaatt
     1021 tcttcatccg tggcttcagc atagtagatt tgggacaggc cgtggtcgga aacgttcgaa
     1081 ccatgatccg caaaatattg gatccgctct tccattcctt ccaacaactc agcgaatgtc
     1141 gtcatttcct tgccggtcgc ctcttgcatt tttccgatga acccagcaaa cttggctgcg
     1201 tcttggatag cgaaagcttc atccggacgg aaaccaggaa cgactgctac atcaaatgtc
     1261 tcgtcttcag cgattgcctt gtgccactcc aacgtatccg tagggttgtc tgtcgtacaa
     1321 atgaaggtga cgttggaatt tttgatcaat tgacgcgctg taagtttttc tgccttcaac
     1381 acgcggttgc attccgcata aatttccttc cagtttgcgc tggtaagtgt ttcttctata
     1441 tggaaatact ttttcaattc caaggctgtc cagtggaaca acgggttccc taccgcattt
     1501 tcagcagtgt aggcccaagc ttcaaatttt tcttcagggg aagcactacc ggtgatcttt
     1561 tcttccggaa ttcccattgc acgcatcgcg cgccatttat aatgatctcc gcccaaccat
     1621 agttcggtaa tgttttcgaa ttcataatct tcagcgatct gctgtgggac caaatggcag
     1681 tggtaatcga agatgggcat gttttccgca tactcgtgat aaagtttttt ggctgtttcg
     1741 gactgtaaca taaagtcgtc atgaataaat gacatgtgaa gaaatcccct tccgagcttt
     1801 attttgaata ttattgatta ttgcgctttg atttctgcca aacgatcagc aaattcttta
     1861 gctgcagctg ttacactggc atagccgcct tcttttaggt ttcgtgtcaa ggcactgccg
     1921 acaccgactg accaagctcc gtttgcaatc catttgtcca tgttctcaat gcttacgcca
     1981 cctgaaggca tcacgtcaac ccaaggaata ggaccatgaa cgtctttgat gaatccagga
     2041 cccaacaaac cacccgggaa cagcttcact acttcgcagc cagtttccaa agccatctga
     2101 atttctgtta cagaaccgca tccaggcaag tatggaacag tgtataggtt gcacattctt
     2161 gaaatctttt cattgaagga agggcttacg ataaattttg ctccgttcat gatcgaaatt
     2221 cttgcagcga cttcatccaa aactgtacct gcacctacaa ccatatctgt acctgcgaat
     2281 tcatcggaca gtttacgcat aacgttctca gcaccaggtg tcgaaaaagt cacctcgatg
     2341 ttcttgatgc ctccttgata agctgctttt gaaatctcat aacctgcttc ctcggaagct
     2401 ccgcgcacta ctgcaaacag gtaattttct tgtaattgag ccagggtatc tctcttgacg
     2461 ctcatttgtc atcctccatt cgtggtttcg cattgtggaa ctcagtatca taactcgagt
     2521 tcattataga atttaccatc ttttatgtca acgaaattcc atcagaaata taaaaatgaa
     2581 tgataaattg tgaaaaattt gtggcatact gttgaatacc tccaaaagaa agagagtctg
     2641 acaaaaaaaa ctccccataa accgcattta tcatgcgttt cgtggggagt tccgctatat
     2701 cggcttgaga tcggaaaaga tcaaaagtga ccgagcaact gtgaaatttt tgtggaatat
     2761 tccaaaattt ttcctgtata gaattcttta tcaaccatgt cagacaaggg caaggatatg
     2821 ctgattgcac ctgcagcaac ttccctagcg ctcgaaaagg ctgcagcgat acagacgatt
     2881 ccctcttcaa actcctcatc atcgagggca taaccatttc ttctgatttc atccagttca
     2941 ctgatgagat cttcgtaagt ggtaatcgta cgcccggttt tttgttccgg aacgatactg
     3001 tcccaatatt ctcggacggc agcgttttga cgggtgctca agagcgcttt cccgacagcg
     3061 gtgcaataaa gcggcgcttt tgtaccgatc tttgattgca tccgtacacc gcgggattca
     3121 ccatatttat cgatatacag cacttcatca ttgtcctcca caaccagatg cacggtctgc
     3181 ctcgtatcat ccgacaattg gcggatcatg cttttagcga cattcaagaa gtcgatgttg
     3241 tggacacgct tgtttccgag atggaacatc ttcaaggaaa tgcgatactg tttcgtatca
     3301 gggttgcgat cgatatagcc gttttccatt aaggaattca ccaagcgatg cgtcgttgcc
     3361 ttgtgcagat ggactttttc gctcaattcc atcagactta gactaggata gtctgccaac
     3421 gtttccaaaa tagccaaagc gcggtcaatt gattgtactt ttggacttgt gtttactgct
     3481 tctttttgta ccattatttt gctcatcctt ttccgttttt agtcttacat ttcttgaatt
     3541 atttggataa tcattcattt ttatacgctt tagatcaaaa aaatacattt tcattcacca
     3601 aacgggtaaa cgtttcacat tgtgatatta tagcaaaaaa aatatagcct gataaccccc
     3661 ttgagcggaa ttatttgtaa aatgtttccg tttccacact gaatcgaaac aatcccctga
     3721 ataaatctca aaaaaataaa aattcgtttc atatttgttt acatttcact aaatcaaaca
     3781 tttttttaat atttactcca ctcctttgac ttgtatacgt tttctgaata tactaatatc
     3841 agattacgaa aagcagtctt acatcctgat acatcactcg cgctattatt ttcgtcctta
     3901 tgttcaaaat attaattttt ttatgggagg tatcttctaa aaatggctaa gaatcatata
     3961 atccacacag ccaaacgtcc attcggattc cgtgatatac tcggatattt tatgggcgac
     4021 ttcggttgca acatgagttt cacgttaatt tccagctata tgttcatttt ctacacacag
     4081 tatatcggga tttcattggc tcactactcc atcatcattc tgattacgaa gatttttgat
     4141 ggcatcaacg atccgattat cggcgctatc gttgatcgga tgggcgcaga taaaaagggt
     4201 gaaaaattca aacaatggat tctgcgaggc tctccattcc ttgcactggc agcaacggtc
     4261 atcttcatag atgcggctga ttggtcttat gccgcaaaac tcggcttgtg tatcggtggg
     4321 tatatcttat gggatttgac ctatacagtc gttaacgtac cttacggcgc tttgagttcc
     4381 gtcatgactt ccaagccttc ccaacgggct gctttatcca acgcgcgttc gtggggatac
     4441 atcatcgccg gcgttccact aggaatcctg attcctttgt tcgcctatga acaagtcacg
     4501 aaaannnnnn nnnnnnnnnn nnntttcgag gcgaaagaat gtttccgatc gctatcatcc
     4561 tgggcatcat ttcgattgtt gcctttgcgt tactttatct gaatacggag gaacgtatcc
     4621 agcacacaga aagtgaaggc gaaaaggtta aattcaatta tttcataaca ttaaaagact
     4681 ttttccgcaa tcgtgcgatt ttgggtattt ctcttgcctc catcgctcag atcctgttca
     4741 taatgggcgg taaccaattg catcaattga cttatcagat gtacttccga gacggaacct
     4801 tgagttctta cgccatcctg actcaaatta ttccactatt cgttggtaca gctctcggtg
     4861 gatctttggt aggcaaactg ggaaaaaaag aattgtcgac ttggccatta ctgggagcca
     4921 tcgctgttta tgcctttctt tatctggtcg aaatcaaaaa tccgattctt tggatcacga
     4981 tgcagatcat cgcaaacagc ttcgccttcg gtttggcgat ctacacttgg gggatggttg
     5041 cggatgcaat cgactaccaa gaatggaaga ccggcatccg aaatgaaggt tctgtgtacg
     5101 caacttactc tatgctgcgt aagatcgcac agggcttcgg ccagtcgttc attcctgcag
     5161 ccattgctat cttgattccg acactggatg tgaaagatgc cttcacctgg acctcagaaa
     5221 acgcgttggc cttacgcgat atggctgttc tattcccgtt gatcggttac attatcatct
     5281 tcatctgctt caagttcatc ttcaacttgg acagaaaaac agttgcagag atgcaagtcg
     5341 ctttaggccg tgacgaagag taatcactca gcagcattgc gcacgcaaaa agaaggtgag
     5401 ctatctccaa ttttgagaca gctcaccttc tttttattcg tattctgcaa agatctcttc
     5461 cggtgtcttc agaacgccac cggtattggc tgatgtaacc aaagcggcgt aacgtgccaa
     5521 ccaacctgtc ttcactttcg atacaaagcg tgtttgcttt ttctttcttt cttcaagtac
     5581 ttcgtcagca acgcttacgt tgatcgtacg gtgctcaaga tcaatgacga tttcatcccc
     5641 atcttcaatc aagccgatcg ggccgcctga agctgcttca ggagagatgt gtccgattgc
     5701 gataccgcga gtggcacctg agaaacggcc atccgtgatc agtgcaacat cacggcctaa
     5761 gccgcgtcct acgatggaag atgtcggtgc cagcatttcc ggcattccgg gtccaccttt
     5821 aggtccttca tagcggatga cgacaacatg gccttttttg acggtgcggt tgtcgattgc
     5881 ttccaccgct tcatcatggc tactgaagca gatcgctttt ccgacgaatt ttttgatgtc
     5941 tgcatccacg ccgccgactt tgatgacgcc gccgaaagga gcgatattac cgttcaggat
     6001 ggacaaccca ccgaccggag aataggcatt ttcgtattta tggatgactt cttcgttctt
     6061 gatttcgtgt cctgcgacat tttcgcggat cgtcttgctg gtgacagtga tacgatccgg
     6121 atgcaggttt ccgtgtttga tcaattcatt catgattgca gggacaccgc ctgcttcatg
     6181 cacatcatgc atggtgtaaa cacttgaagg tgca
//
LOCUS       DAOMUJ010000046         6116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3480_length_6116_cov_2.874443, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000046 DAOMUJ010000000
VERSION     DAOMUJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3480_length_6116_cov_2.874443"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLT73_02085"
     CDS             <1..555
                     /locus_tag="PLT73_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19202.1"
                     /translation="DEATTTIGISLLGERGGSIILSFVLIAILGVTNGVIMGMLRLPQ
                     ALAEKNMLPNSAKVKRIDPKSQLSPAAAQIAFAISFVWMVLHYLTQKSAILGGGDVSE
                     IAIVFSYLTYVILYLKVISMKKEGVITSPFFGWIMPVFAIMGSAIILIGGIVSNPTYV
                     PVFILFCFVVCFIGYKYYEKKITV"
     gene            complement(874..2232)
                     /locus_tag="PLT73_02090"
     CDS             complement(874..2232)
                     /locus_tag="PLT73_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRM19203.1"
                     /translation="MLRTDNILDTIRMFEEDNLDIRTITMGISLLDCIDSDGEKARER
                     IYKKITTKAKNLVSVAQEIEDTYGVPITNKRISVTPIALIAGASDDTDYVAYAKTLDA
                     AAKEVGVDFIGGFSALVEKGYNKGDKILIDSIPEALAVTDKVCSSVNIGSTKAGINMD
                     AVKLMGEIVLATAEKTADRNGFGCAKLVVFANSVSDNPFMAGAYHGIEEADCEIHVGI
                     SGPGVVKRALEKVKGEPMDIVADTIKKAAFQITRMGQLVGNIASQKLGVPFGIVDLAL
                     APTPAVGDSVGEILEEFGLGVVGTHGTVAALAVLNDAVKKGGVMACSHVGGLSGSFIP
                     VSEDHRMIEAAAANQISLDMLLAMTAICSVGLDMIAIPGNTPATTIAAMIADEAAIGV
                     QNNKTTAVRVIPAIGCGVGDFVEFGGLLGRAPVQPVHEESSADFINRGGRSPAPIHSF
                     KN"
     gene            complement(2245..2514)
                     /locus_tag="PLT73_02095"
     CDS             complement(2245..2514)
                     /locus_tag="PLT73_02095"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM19204.1"
                     /translation="MRAIMTVTGKDHTGIVASVSVELARLGVNILDISQTIMDEYFTM
                     ILMVELNEANNSVKEVKEAMKKVEEEQGLVIRLQAEDTFHAMHRI"
     gene            2805..3683
                     /locus_tag="PLT73_02100"
     CDS             2805..3683
                     /locus_tag="PLT73_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRM19205.1"
                     /translation="MQLIHFSEAGQSRIGIKTERGFLNAAKAAAALSLPVPSYIDDVL
                     KDPVQAAQLDAILAQADTLTDPVYYVSLDEVTFLPVLSRPGKILCIGKNYAAHVAETK
                     SEAPKKPLVFSKFTSALAAHKENIPIPAGTDKVDYEVELVAVIGKKAYCVSPEEALEH
                     VAGYTIGNDVTARDWQKGSPQWVLGKSPDKFAPLGPVYVTADELDPTHLDISLKLNGE
                     VRQNSNTKHLIFDIATIISYISQHFALEPGDIIFTGTPDGVILGYPEDEQVWLKPGDV
                     IESTIEGIGTLVNSFC"
     gene            complement(4010..>6116)
                     /gene="carB"
                     /locus_tag="PLT73_02105"
     CDS             complement(4010..>6116)
                     /gene="carB"
                     /locus_tag="PLT73_02105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM19206.1"
                     /translation="EKGDRSLGTQMKATGEVMAMGHTFEESMLKAVRSLEYGVNHLAL
                     PKDQGQTVTMAEIEQNIRVACDERLFYLGEALRRGVTPETIHEWSEIDYFFLYKFKHI
                     IDLEKEVAANVKDLETLREAKKYGFSDEIIAELWGMASEDIYALRKANGIVPVFKMVD
                     TCAGEFESFTPYFYSTYEMEQESFPSDREKVIVLGSGPIRIGQGVEFDYATVHAVWAI
                     QEAGYEAIVVNNNPETVSTDFSISDKLYFEPLTEEDVMAIIDLEQPLGVVVQFGGQTA
                     INLADKLVKHGVKILGTSLEDLDRAEDRKLFEQLLRDLDIPQAPGKTAVTVEEAVAVA
                     DEIGYPVLVRPSYVLGGRAMQIVYNQADLEKYMREAVVASPERPVLVDHYLIGQELEV
                     DAICDGETVLIPGIMEHIERAGVHSGDSIAVYPPQNLSAELIQTIEDYTIRVAQGLNT
                     IGLVNIQFVISNGVVYIIEVNPRASRTIPFLSKVTGIPMANLAMQGILGIKLKDQGYR
                     TGLVPSKSGVYVKMPVFSFNKLKKVDTVLGPEMKSTGEIIGKDAILSKALYKAFRAAN
                     IQIPEYGTALLTIADKDKHEILPLAKRLVAVGYRLLATQGTGETLLEAYVPVTILRNG
                     EAKRENILKSMHEGKIQFVINTMTEGKTEETDGYFIRREAADNNLPCLTSLDTTEALL
                     QAMEMMHFQLQAVGTEPVF"
BASE COUNT         1387 a         1674 c         1473 g         1582 t
ORIGIN      
        1 gatgaggcga ccacaaccat cggcatttcc ttgctgggcg agcgcggcgg cagcatcatt
       61 ctttcctttg tattgatcgc tattttgggt gtaacaaacg gcgtaatcat gggcatgctg
      121 cgtttgcctc aagcgctcgc tgaaaaaaat atgctgccta acagcgctaa ggtaaaacgg
      181 atcgatccaa agagccaact gtccccggca gccgcacaaa ttgcctttgc aatctccttt
      241 gtgtggatgg tgttgcacta tttgacccaa aaatccgcga ttctgggtgg cggagatgtc
      301 agcgaaatcg cgattgtctt cagttatctg acctatgtca tcctttatct gaaagtcatc
      361 agcatgaaaa aagaaggggt catcaccagt cctttcttcg gttggatcat gccggtattc
      421 gcgatcatgg gctccgccat cattctgatc ggcggcatcg tgtccaatcc gacttatgtg
      481 ccggttttca tcctgttctg cttcgtggtt tgcttcatcg gatataaata ctacgagaaa
      541 aaaattacgg tataacatta atgaagggct gctcatgccg ggcagccctt cttcgttttt
      601 ttatgtctgg acagtatccg ttcctggttg ccgccaaagg agtgtgccgg aatcgaacac
      661 ccagctccgg ccaaggtgcc gctccccgga gaagagagac aatccccacg atgtcctcct
      721 gcgaagcctt gctcgcagga ggatgcagac taaatgttgg ccgaactccg gggaaacctc
      781 ccctcaccgg agctcacgct cacgtccttc ggtcgcactc aaaaaaaatg gagccgtccc
      841 gcgaagggac aactccattt ttttgactga catttagttt ttgaatgagt ggatcggtgc
      901 agggctacgt ccaccgcggt tgatgaagtc agccgaactt tcttcatgga caggttgtac
      961 gggagcgcgt ccgagcaaac caccgaattc aacaaaatca cccacaccac agccgattgc
     1021 cggaatcacg cggacagctg tggttttgtt gttttggacc ccgatggccg cttcatccgc
     1081 gatcatcgcc gcgatcgttg tcgccggcgt attgccaggg attgcgatca tatccaaccc
     1141 aaccgaacag atagccgtca tcgccaacaa catatccaac gagatttggt tggcagcagc
     1201 cgcttcgatc atgcgatgat cctcagatac tgggatgaac gagccggata agccaccaac
     1261 gtgactgcaa gccattacgc cgcctttttt gactgcgtca ttcagtacag ccaaagcagc
     1321 aaccgttcca tgggtaccga ctacgcccaa cccaaactct tcaaggattt cccctaccga
     1381 gtcgccgact gcaggagtcg gggccaaagc caagtcgacg ataccgaatg gcacgcccaa
     1441 tttttgcgat gcgatattcc cgaccaattg ccccattcgg gtgatttgga acgccgcttt
     1501 tttgattgtg tcggcaacga tatccatcgg ttcgcctttc actttttcca aagcacgttt
     1561 gacgacacca ggtccggaaa tgccgacatg aatttcgcaa tccgcttctt cgataccatg
     1621 gtaagcgccg gccatgaatg gattatcact tacggagttt gcaaaaacaa ccagttttgc
     1681 gcaaccgaag ccgtttctgt ccgctgtttt ttccgctgta gcaagaacga tttcgcccat
     1741 caatttgaca gcatccatgt tgatgcccgc tttagtggat ccgatgttca cgctgctgca
     1801 gaccttgtcc gtcactgcta aagcttccgg aatcgagtcg atcaatatct tgtcgccttt
     1861 gttgtagcct ttttcaacta gagctgaaaa gccgccgatg aagtcgacgc cgacttcctt
     1921 cgccgccgca tccaaggttt ttgcgtaggc tacatagtcc gtgtcatcgc ttgcgccggc
     1981 aatcaaggcg atcggtgtca ccgaaatccg cttgttcgtg atcggaacgc catatgtatc
     2041 ttcgatttcc tgcgctacgc tgaccaagtt cttcgcttta gtggtgattt ttttgtatat
     2101 tctttcacga gctttttcac catcgctgtc gatgcagtcc aataaagaga tccccatggt
     2161 gatggtacgg atatccagat tgtcctcttc gaacatgcgg atggtgtcca agatattgtc
     2221 agttcttaac attcaaatgt ccccctagat ccgatgcatt gcatggaatg tgtcttctgc
     2281 ctgtaaacgg atgaccaagc cttgttcttc ttcaactttt ttcatcgctt ctttgacttc
     2341 tttgaccgaa ttattcgcct cgttcaattc aaccatcaaa atcatggtga agtactcatc
     2401 cataatcgtc tgtgaaatat ccaggatatt tacgcccaga cgcgccaatt ccaccgatac
     2461 gcttgctacg atcccagtat ggtctttacc tgttacggtc atgattgctc tcatcttttt
     2521 tcgcccctgc ttctttagtt ttttgtcgag tctattatac cattttacag caatatgaaa
     2581 atccaattaa aattttatgt ttcctattct atcacatcta aagggaatga ctactgtttt
     2641 tttgggaaac ggaaaccggt aaccgctatc cagcggaatt tttttgtgtt ctttcccgaa
     2701 acacgaaaat tgttgcagat tccgcattaa atctatcgct ttttgagatt aattgctaca
     2761 ctggatataa gcaaattcta atctaatctc aagggggttt tccaatgcaa ttgattcatt
     2821 tctcagaagc tgggcagagc cgcatcggca tcaaaacgga gcgtggtttc ctgaacgcag
     2881 caaaagcggc tgcagcttta tccttgcctg ttccttccta tatagatgac gtattgaagg
     2941 atccggtaca ggccgcccaa ctggatgcta ttctggccca agcggacacg ctgacggatc
     3001 cagtctacta tgtttccttg gatgaggtca cattcctgcc cgtcctttcc cgtccgggca
     3061 aaatcctctg catcggcaag aactatgccg cccatgttgc ggaaacaaaa agtgaggccc
     3121 cgaaaaaacc gcttgtcttc agcaagttca cgagcgctct ggctgcccat aaagaaaaca
     3181 tcccgattcc ggccggcacg gacaaggtcg attatgaagt cgagttggtg gctgtgatcg
     3241 gcaaaaaagc ttattgcgtc tccccggaag aagccctaga gcacgtagcg ggctacacca
     3301 tcggcaacga cgtgactgcg cgcgactggc aaaaaggatc tccgcaatgg gtgctgggga
     3361 aatcacctga caaatttgcg ccgctcggac cggtctatgt gactgccgat gagctcgatc
     3421 cgacccatct tgatatctcc ctcaagctaa acggagaggt ccgccagaac agcaacacaa
     3481 agcatctcat tttcgatatc gcgacgatca tttcctacat ttcccaacac tttgccttgg
     3541 aacccggcga cattatcttc accggaacgc cggatggggt catcctcggt tatccggaag
     3601 acgaacaagt ctggctgaaa ccaggtgatg tcattgaatc caccattgaa ggcatcggga
     3661 cattggtgaa tagtttctgc taaaaaacat ccaaagaggc tctctcgtaa tgagaaagcc
     3721 tcttcttttt gcgcctgctg agcggaggcg tgctgataat aaataagagc taatccgtcc
     3781 cagctccggc gaagaacctc ctcaccggag cacatcggta acaatcggac tcccctccca
     3841 cgaagccacc ttcatcgaag aacgtaaacg aaatgaatcc tgaactccgg cgaagcctcc
     3901 gttcgccgga gaagaagaca gccgatatgc gcgcaaaaaa tgaccgaata acgacgataa
     3961 agggccttcc tcaaagtgac gaggtagccc tttatttaaa ctgtcctatt taaaaaactg
     4021 gttcggtccc gactgcctgc agttggaaat gcatcatttc catcgcctgc agcagcgcct
     4081 cggttgtatc cagcgaggtc aggcacggca ggttgttgtc cgcggcttcg cggcggatga
     4141 agtagccgtc ggtttcctcg gtcttgcctt cggtcatcgt attgatgacg aactggattt
     4201 tcccttcgtg catgcttttc aggatatttt cccgtttggc ttcgccattt cggagaatcg
     4261 tgaccggcac ataggcctcc agcaaggttt cccctgtccc ctgtgtagca agcagacggt
     4321 acccgaccgc aaccaagcgt ttcgccagcg gcaggatttc gtgtttgtcc ttgtcggcaa
     4381 tcgtcagcag cgccgtcccg tactcgggaa tctggatatt ggcggcacga aatgctttgt
     4441 acagcgcttt ggaaaggatg gcgtcctttc cgatgatctc ccctgtggac ttcatttccg
     4501 gccccaatac ggtatcgact ttcttcagtt tattgaaact gaaaaccggc atcttcacgt
     4561 atacgcctga ttttgacgga accagaccag tccgatagcc ttgatctttc agtttgatgc
     4621 caaggatgcc ttgcatcgcc aaattcgcca tcgggatgcc ggtcactttg ctaaggaatg
     4681 ggattgtccg gctcgcgcgc ggattcactt cgatgatgta gacgacaccg ttgctgatga
     4741 cgaactggat gttcaccaaa ccgattgtgt tcaacccttg cgccacgcgg attgtgtaat
     4801 cttcaatcgt ttggatcagt tcagccgaca gattctgcgg cgggtaaaca gcgatcgagt
     4861 cgcccgagtg gacgccggcg cgttcgatgt gttccatgat gcccgggatc aggacggttt
     4921 cgccgtcgca gatcgcatcg acttccagct cttgtccgat caggtaatga tcgaccagga
     4981 ccggccgttc gggactggcc acgaccgctt cgcgcatgta tttctccaaa tcggcttggt
     5041 tgtagacgat ctgcatcgcc cggccgccca atacatagga cggacgcact aagaccggat
     5101 agccgatttc gtcggcaact gcgaccgctt cttcaacggt cacagctgtt ttgccgggtg
     5161 cttgcgggat atccaaatcg cgcaacaact gttcgaacag tttacggtct tcggcacggt
     5221 ccagatcttc caggctcgtg cccaggatct tcacgccgtg tttgaccaat ttgtcggcca
     5281 gattgatggc cgtttggcca ccgaactgca caacgacgcc caacggttgt tccaggtcga
     5341 tgatcgccat cacgtcttct tcggtcaagg gttcgaaata cagcttgtcg gagatcgaga
     5401 agtccgtcga aacggtttcc gggttgttgt tgacgacgat cgcttcgtag ccggcttcct
     5461 ggatcgccca gaccgcatgc acggtcgcgt agtcgaactc gaccccttgg ccgatacgga
     5521 tcggaccgga gcccaagacg atgacttttt cgcggtcgct cgggaatgat tcctgttcca
     5581 tttcatacgt gctgtagaag tacggtgtga aggattcgaa ttcgcccgca cacgtatcca
     5641 ccatcttgaa gacagggacg atgccgttgg ctttacgcaa cgcgtagatg tcttccgaag
     5701 ccatgcccca cagttccgcg atgatttcat cgctgaagcc gtatttcttc gcttcgcgca
     5761 acgtctccag atccttcaca ttggctgcca cttctttttc aagatcgatg atgtgcttga
     5821 atttgtacag gaagaagtag tcgatttcgc tccattcgtg gatcgtttcc ggtgtcacgc
     5881 cgcggcgcaa cgcttctccc agatagaaca gacgctcgtc gcaagcaacg cgaatattct
     5941 gttcgatttc tgccatcgtc acggtctggc cttggtcttt cggcaaggcc aaatggttga
     6001 cgccatactc caaagagcgg acggctttca gcatcgattc ctcgaacgta tggcccatgg
     6061 ccatgacttc gcctgtcgct ttcatctgcg tgccgaggct gcggtcgccc ttttcg
//
LOCUS       DAOMUJ010000047         6115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3482_length_6115_cov_2.841419, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000047 DAOMUJ010000000
VERSION     DAOMUJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3482_length_6115_cov_2.841419"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PLT73_02110"
     CDS             complement(<1..1152)
                     /locus_tag="PLT73_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM19207.1"
                     /translation="MQELKGSRMGTEKIPKLMMELAIPSIIAQVINILYNIVDRMYIG
                     HIPDVGAVALTGLGISAPLVLIISAFSSFAGGGGAPLAAIALGKNDKNEAEKILGNAF
                     SMLTLMAVVLTIVFTLIKEPLLYLFGASDVTFNYANDYTSIYIMGTIFVQYALGLNLF
                     ITSQGKTKNAMFSVLIGAIANIILDPIFIFGFDMGVQGAAIATVISQTLSAIYVLRFL
                     TSEHSIIRIRKKNMGLEIGLIKRIVSLGISPFIMQATESAIVIVFNTGLLKYGGDLYV
                     GSMTIMQSIMQLLTVPVQGFTQGVQPIISYNYGAGNYKRVRETVRHSMKVTVGLTVSY
                     FLLVLFVPGLFARIFTTNSDLLALVTRVLPIYMGGMWLFGVQMSAQMFFV"
     gene            complement(1155..1589)
                     /locus_tag="PLT73_02115"
     CDS             complement(1155..1589)
                     /locus_tag="PLT73_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM19208.1"
                     /translation="MKEKKNLLISLSKLSKVYRAEVKVEMSASDFSPNELDLITFLSN
                     NEMDTSKEIADFLGLSKSLIARSVDSLVAKGYLETNVDEGDRRYIHLVLTDRAKPIAD
                     RLRNIRKQFIASMTEGINQEQFTQFEAMLEKMVANVERKKEE"
     gene            complement(1847..2647)
                     /locus_tag="PLT73_02120"
     CDS             complement(1847..2647)
                     /locus_tag="PLT73_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19209.1"
                     /translation="MNKLEIEKLHFAYDKTTPVIKDINLVLDDRKTAIIGQNGSGKTT
                     FVKLLKGLLRPDSGRILLDGIDLKTLTVAQISKKIGLVFQNPDDQIFKNTVLDEVMVG
                     PLNIGLSLDEARASSRAALAQVELVDVEKVNPYDLNLAQRKMVALASILAMDTPIVIF
                     DEPTMGQDATGKAIIKKVIENLQAEGKAVLCILHDMDFAAAVFERVVVFSQGQLLLEG
                     GAREVFSKKDLLQQAYLDQPQAMKIAQALGMQGYLLSVEEVISALEKP"
     gene            complement(2640..3491)
                     /locus_tag="PLT73_02125"
     CDS             complement(2640..3491)
                     /locus_tag="PLT73_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19210.1"
                     /translation="MSIIEVKDLKYRYPDTTKLALDGISFSVEEGEFIGLIGRNTAGK
                     STLCYALSGLVPHFFKGAYGGSVMIAGLDVRTTDVSEVTAAAGLVFENPFSQITGSRF
                     TVYEEIAFGLENMGLPRDEIIKRIEESLDLLDIRKVKDKNPFDLSGGQMQRVAIAGVV
                     AMKPKVLILDEPTSQLDPQGSDEVFKVVENLTKEGITIIMAEQKMEKIAQYADRVLLL
                     DDAKLIAYDTPEAIFSREDLASLGVQPPAVTAIARHLNKRKANGHYPVELDELKGLLA
                     EEGVPHE"
     gene            complement(3488..4255)
                     /locus_tag="PLT73_02130"
     CDS             complement(3488..4255)
                     /locus_tag="PLT73_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM19211.1"
                     /translation="MDAMTLYVPRNSPIHHLDPLTKMLYVVVSIAAAYLLDDLWQVAA
                     VMLTSFGILLVGKVFRNILPVIALSFLLILSIVIVQGFFNPANETLLYELGPIKFYKE
                     GLIIAFRLTMRVINMVSAFGVLVLTTSPTELVERLQQKGMSPKIGYVILSVLQIIPQL
                     RATYGKIQDAQRSRGMETEGSLFVRVKAFFPLLGPVILNALNDTRERAIALDVRGFDR
                     DTPKTYLNEAKSYRYSTFLNILLLVILAGLIVWRVMG"
     gene            complement(4268..4876)
                     /locus_tag="PLT73_02135"
     CDS             complement(4268..4876)
                     /locus_tag="PLT73_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM19212.1"
                     /translation="MNAETKNKLNYKLSTASIVLIPIAIGINYLGKYIAGVLRLPLWL
                     DSIGTVLSGMLAGPVIGAASGIINNVIYGVTADPISTVYAVTSAVIGLMAGLFAAKGW
                     FKDIKTVLLAGLIIGLVAATVSTPLNILFWGGQTGNVWGDALYALLISNGQPQWLASF
                     LDSIVVDVPDKLVTVLISYFIFRGLPKKLTNTFLKDGAIEEL"
     gene            complement(4878..5633)
                     /locus_tag="PLT73_02140"
     CDS             complement(4878..5633)
                     /locus_tag="PLT73_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRM19213.1"
                     /translation="MEQTLQAHIQLSADLDVKYALLPGDPARVERAKLLLEDAVDLAF
                     NREYKSVLGTYQGMKVLVISSGIGGPSTAIAIEELARIGIEGIIRIGSCGSLHPAVHL
                     GDVIIATAAVRDDGASKAYVDSAYPAVADLDLLIHLRAIAETERIPHRLGIVRSHDSF
                     YTAREAQLTAYWSGNGIIGSDMETATLFVVGALRGLKTASLLNVVVGPEEGMAEGVRQ
                     FVSGEAATKQGEQNQIKLALSAFHAWHQAKGGE"
     gene            complement(5845..>6115)
                     /locus_tag="PLT73_02145"
     CDS             complement(5845..>6115)
                     /locus_tag="PLT73_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRM19214.1"
                     /translation="LIGGVAGIIVAGVIFAGIFGTVVSGLIFRIFRIEEPIAKGLALG
                     AASHAMGTAESLKASELQGAMSSLAMVVSGVVMVGLIPLAALFVQ"
BASE COUNT         1570 a         1691 c         1312 g         1542 t
ORIGIN      
        1 gacaaaaaac atttgcgcgc tcatctgcac tccgaacagc cacattccgc ccatataaat
       61 cggcagaaca cgggtgacta aagccaacaa atccgaattt gtcgtaaaga tgcgtgcgaa
      121 cagacccggc acgaacagca ccaataaaaa gtaggagact gtaagtccaa ccgttacctt
      181 catggaatgg cgcacagttt ctcgcacacg cttgtaattc cctgctccat aattataact
      241 gatgattggt tgaacacctt gcgtaaaacc ttgtacaggc actgtcagta attgcatgat
      301 gctttgcatg attgtcatgg aaccaacata aagatcgccg ccgtatttca gcagtcctgt
      361 attgaagaca atcacaatcg cactttcggt cgcttgcata atgaatggcg aaattcccaa
      421 ggaaacaatt ctctttatca gcccaatctc tagccccata ttcttcttgc ggatccggat
      481 aatcgaatgc tcggacgtca agaatcggag gacgtagatc gcactcaggg tttgggaaat
      541 gactgttgca atcgcagcgc cctgaactcc catatcaaaa ccgaaaataa aaatcggatc
      601 caggataata ttggcaatag ctccaatcag tactgaaaac atagcgtttt ttgtttttcc
      661 ttgactggtt ataaatagat tcaggcctaa tgcatattgt acaaaaattg tgcccattat
      721 atagatggaa gtatagtcat tcgcataatt gaaagtaaca tcactggcac cgaataaata
      781 taataatggc tctttgatca aggtaaagac aatagtcaaa acgactgcca tgagcgtgag
      841 catggaaaaa gcattgccga ggattttttc ggcctcattt ttgtcgttct tgcctaacgc
      901 aatggccgcc aaaggagccc cgccacctcc ggcaaaactg ctgaacgcag aaatgatcag
      961 cacaagcggt gcgctgattc ccaacccagt caatgcaaca gcccctacat cgggtatatg
     1021 tccgatgtac atcctatcca caatgttgta aagaatattg atgacttggg caataatgct
     1081 cggtatggcc agttccatca tcagtttagg gattttctcc gtccccatgc gtgatccttt
     1141 taattcttgc acgattactc ctccttcttc cgttccacat tcgctaccat tttttcaagc
     1201 atcgcttcaa actgcgtgaa ctgttcttga ttgattcctt ctgtcatcga cgcgataaac
     1261 tgtttccgga tattccgtaa tcggtccgct atcggctttg cccgatcagt caatacaaga
     1321 tgaatatagc gacggtctcc ctcatcaacg tttgtctcca agtacccttt tgcgacaagc
     1381 gaatcgaccg atctggcaat caaagacttt gatagtccca ggaaatctgc tatttctttg
     1441 gatgtgtcca tttcgttatt tgacaaaaag gtaatcagat ccaactcgtt cggagaaaaa
     1501 tccgaagcgg acatctctac cttcacttct gcccgataca ctttcgacag cttggacaag
     1561 ctgatcagca gatttttctt ttctttcata aatcttacgc ccccatgtat tcaaactaaa
     1621 actattttac cgctaaatag ttcacttgta taccatccgt ttatgagctg cttttcacgg
     1681 cagaacaacc ttcccatcaa aaaaaacaca cgcaacaata cgaactcgca aagagcccta
     1741 ttgctgtatg tgttttattg atagctgaca gagtttccgc agatgattcc gtctcatctt
     1801 tacaatccgc aaggtacgat tccactattt cggtcctttg ctgaaatcat ggtttctcca
     1861 aagcggatat cacttcttca acgctcaaaa gatacccttg cattcccaaa gcttgcgcga
     1921 ttttcatggc ttggggttga tccagatagg cttgctgcag aaggtctttt ttgctgaaga
     1981 cttcccttgc accgccttcg agcagcaatt gcccttggct gaagacgacg acgcgttcaa
     2041 aaactgccgc cgcaaaatcc atgtcatgca gaatgcacag tacggctttc ccttctgcct
     2101 gcaggttttc gatcactttt ttgatgattg ctttcccggt agcgtcctgc cccatcgtgg
     2161 gttcatcgaa aatgacgatg ggcgtgtcca tcgctaaaat cgaagccaac gccaccatct
     2221 ttctttgcgc cagattgagg tcgtatggat tcactttctc gacatccacc aattctactt
     2281 gagccaatgc cgcgcgtgaa ctggctcttg cctcatccaa agaaagtccg atgttcaacg
     2341 gaccgaccat cacttcatcc aaaaccgtat tcttgaaaat ctggtcatcg gggttctgaa
     2401 aaaccagacc aatcttcttg gatatctggg caaccgtcaa agtttttaag tcgatcccgt
     2461 ccagcaggat cctgccggaa tcgggacgca aaagtccctt cagcagtttt acgaaggtgg
     2521 tttttcccga gccgttctgt ccgatgatgg cggttttgcg gtcgtccagc accagattaa
     2581 tgtcttttat gacgggtgtt gttttgtcgt atgcaaaatg cagcttctct atttccagtt
     2641 tattcatgag ggacaccttc ttccgcaagc aagcctttca gctcatccaa ttccacagga
     2701 taatgcccat tagccttccg cttgttcaaa tggcgggcga tcgcggtcac ggcgggcggc
     2761 tgtacgccca gagacgccaa gtcctcgcgc gaaaaaatag cttcgggcgt atcatacgcg
     2821 atcaacttgg catcgtccaa aagcaggacc ctgtctgcgt actgcgcgat tttttccatc
     2881 ttttgctcgg ccatgatgat ggtgatgcct tctttcgtaa ggttttccac aactttgaag
     2941 acctcatccg acccttgcgg atccagttgg gaagtgggtt catccaatat cagcactttc
     3001 ggcttcatcg cgaccacacc ggcgatcgca acccgctgca tttgcccgcc ggaaagatcg
     3061 aacgggttct tgtccttcac tttgcggata tccagcaagt ccaaactttc ttcgatccgc
     3121 ttgatgattt cgtcgcgcgg cagtcccatg ttttccagcc cgaatgcgat ttcttcatat
     3181 actgtaaaac gcgagccggt gatctgggaa aacgggtttt cgaacaccaa tccggctgct
     3241 gcagtgactt cgctcacatc cgtcgtgcgc acatccaaac cggctatcat caccgatccg
     3301 ccgtaggccc ctttgaagaa gtgcggcacc aagccgctca gcgcatagca caaagtcgat
     3361 ttgccggcgg tgtttcttcc gatcaggccg atgaattcac cttcctcgac cgaaaagctg
     3421 atgccgtcca acgccaattt ggtcgtgtcg ggataacgat atttcagatc cttcacttct
     3481 atgatactca tcccatcacc ctccaaacga tcaaaccggc cagaatcacc aacagcaaaa
     3541 tattcaaaaa agtactgtaa cgataggact tcgcttcatt cagataggtt ttcggagtgt
     3601 ccctgtcgaa gccgcggaca tcgagcgcaa tcgctctctc cctagtatcg ttcagggcgt
     3661 tcaggatgac cggtcccaac aatgggaaga atgcctttac ccgaacaaac aggctgccct
     3721 cagtctccat cccgcgcgac cgttgtgcat cctgaatttt gccgtaagtg gcccgaagct
     3781 gcgggatgat ctgcagaacc gaaaggatga cgtagccgat tttcggggac atgcctttct
     3841 gctgcagccg ttcaaccagt tccgtcggtg aagtcgtcag caccaacact ccaaaggcgc
     3901 tgaccatatt gatgacgcgc atggtcagac gaaaggctat aatcaggccc tctttgtaga
     3961 attttatcgg gcccaattca taaagtaacg tttcgttggc aggattgaaa aaaccttgca
     4021 caatgacgat cgacagaatc aataaaaaac tgagtgcaat caccggcaaa atattacgga
     4081 ataccttccc taccaacaag ataccaaaac tggtcagcat gactgccgca acttgccaca
     4141 gatcatccaa taaataggcc gcagcgatgc tcacgacgac ataaagcatc ttcgtcaacg
     4201 gatccaaatg atggatcggc gagttgcgcg gtacatacaa agtcattgca tccattcaaa
     4261 tcagccccta taattcttct atcgcgccat ccttcaggaa agtgttcgtg agtttcttcg
     4321 gcaagcccct gaagataaag taactgatga gaaccgtcac tagtttatcc ggcacatcca
     4381 caacgatgct gtccaggaaa gaagccagcc attgcggttg cccattcgaa atcagcaaag
     4441 cgtagagggc atccccccag acattaccgg tctgtccgcc ccaaaagagg atgttcaaag
     4501 gcgtcgagac tgtggcggcc accaagccga tgattaaacc tgccaacagc acggtcttga
     4561 tgtccttgaa ccagccttta gcggcaaaca gacccgccat caaaccgatg actgcactgg
     4621 tgactgcata cactgtcgaa atcggatcgg ccgtcacgcc gtagatgacg ttattgatga
     4681 tcccggaagc ggcaccgatt accggtcccg ccaacatacc ggacaatacc gttccgatcg
     4741 agtccaacca aagcggcaga cgcaatacgc ccgcgatgta tttgccgaga taattgatcc
     4801 cgattgcgat cgggatcaag acgatggaag ccgtagacaa cttgtagttc agcttgtttt
     4861 ttgtttctgc attcatttca ttctcctcct tttgcctgat gccatgcatg gaaagcggat
     4921 aatgccagtt tgatttgatt ctgttcccct tgcttggtcg ccgcttctcc tgaaacgaat
     4981 tgacggacgc cttccgccat cccttcttcc ggaccgacaa cgacattcag cagcgatgcg
     5041 gtcttcaatc ccctcagcgc cccaacaaca aacaacgtgg ctgtttccat atccgacccg
     5101 atgatgccgt tgccgctcca ataagcagta agttgcgctt cacgggcggt gtagaaactg
     5161 tcgtggctgc ggacaatccc caaacgatga gggatccgct ccgtttccgc aattgcccgc
     5221 agatgaatca acaaatccag atcggcaaca gcgggatatg cggaatcgac ataggccttg
     5281 ctggcgccat catccctgac cgctgccgtc gcgatgatca catcgcccag atgcactgct
     5341 ggatgcaaag acccgcagct gccgatgcgg atgatgcctt ctattccgat ccttgccaac
     5401 tcttcgatgg ctatcgctgt ggatggtcca ccgatcccgg aggaaatgac caataccttc
     5461 attccttgat aagtccccag aacactcttg tactcacggt tgaacgccaa atcgacagcg
     5521 tcctccaaca gtaatttcgc tctttcaacg cgggctggat cacccggcaa cagcgcatat
     5581 ttcacatcca aatcggctga taattggata tgtgcctgca gtgtttgttc catttctcca
     5641 ccgtcctttt cgcaacgttc gactccggaa tagcccgaca aaacttcaag atactgatac
     5701 ccattatgag ttcagtatag catagagccc aacgtctggc accggataac ccttgatgga
     5761 aagacggtct tcccggcgca caaaaagaag cagtctctta gattaacaga ggctgcttct
     5821 tatgttttac cttagttgtt gcccttactg gacgaacagc gccgcaagcg ggatcaaccc
     5881 caccatcacg acgcccgata cgaccatcgc taggctgctc atggcaccct gcagttcgct
     5941 tgctttcaac gattcagctg tccccatggc gtgggaagcc gcacccaaag ccaacccttt
     6001 cgcgatcggt tcttcgattc tgaagattct gaaaatcagc cccgaaacca ccgttccgaa
     6061 aatgcccgcg aagatgaccc ctgccactat gatccctgcg acaccgccga tcagt
//
LOCUS       DAOMUJ010000048         6058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3523_length_6058_cov_2.240546, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000048 DAOMUJ010000000
VERSION     DAOMUJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_6058_cov_2.240546"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..540
                     /gene="infC"
                     /locus_tag="PLT73_02150"
     CDS             37..540
                     /gene="infC"
                     /locus_tag="PLT73_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRM19215.1"
                     /translation="MMVNDGIRARELRIIGSDGEQLGVKSKSDALQIAEAANLDLVLV
                     SPNANPPVARIMDYGKFRFDQQKREREARKNQKVVSLKEVRLSPSIDENDFQTKLRNA
                     RKFLEKGDKVKASIRFKGRAITHKEIGQRVLDRLASELTDVATIESHPKMDGRSMFLM
                     LAPKADK"
     gene            571..771
                     /gene="rpmI"
                     /locus_tag="PLT73_02155"
     CDS             571..771
                     /gene="rpmI"
                     /locus_tag="PLT73_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003646917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRM19216.1"
                     /translation="MPKQKTHRGSAKRFKRTGNGGLKRWSAFTSHRFHGKTKKQRRQL
                     RQASMVSASDIKRISQQLSQMK"
     gene            828..1187
                     /gene="rplT"
                     /locus_tag="PLT73_02160"
     CDS             828..1187
                     /gene="rplT"
                     /locus_tag="PLT73_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRM19217.1"
                     /translation="MPRVKGGTVTRKRRKKIIKLSKGYYGSKHTLFKTAKEQVMKSYT
                     YAYRDRRQKKRDFRRLWIARINAAARINGMSYSTLIHGLKLAGIEMNRKMLADLAVTD
                     AAAFAAVAEQAKAALAK"
     gene            1299..2384
                     /locus_tag="PLT73_02165"
     CDS             1299..2384
                     /locus_tag="PLT73_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRM19218.1"
                     /translation="MEEKELQMLIDLTDARGVPGNEGEVREVFRRYAEPFAESFSQDG
                     LGGLFAKHTGAEEGPTILLAGHLDEVGFMVTSITEKGFVKFQTLGGWWNQVMLAQQVE
                     ITNSKGERCHGVIGCKPPHVLTPEARKKPYEIKDMFIDIGASSKEQVAEWGIKPGDMI
                     TPYISFKRMNNSKFLLAKAWDNRIGLAVALKVLENLANTAHPNIIYSGGNAQEEVGLR
                     GAKTATHMIRPDIAFALDTGTAGDTPGMTPQEADSELGKGPQLLIYDASMIPHRGLRD
                     FIVGVAEECEIPFQYTVITGGGTDAGAQHQSLDGIPSFAITVPVRYLHSHTSMIHEDD
                     YLNAVKLVTEIVKRLDAETVRQIRENV"
     gene            2503..3930
                     /gene="hemG"
                     /locus_tag="PLT73_02170"
     CDS             2503..3930
                     /gene="hemG"
                     /locus_tag="PLT73_02170"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRM19219.1"
                     /translation="MEKLKRRIVIIGGGLRGLTAAYEIDKAIRKQNLPFEYVVLEDRS
                     AVGGMIHTIEMDGYAFDVGASAFDSRRTDISGFFRELGLESEIQFSKGGKLVLFDGNA
                     IIDDVMSTYHGIPLFPNGIWQANGLTFDAKLRAFMNSLFLSKNLHENPEYSTDNFLEF
                     RLGREVVDYMAEPYYPDSVYGSMELMPVKLFDDNLVTIFGRAHIGKNKKEQLLRFADG
                     SGQEYTFKGGLTTLIKSLAQHSEGHLYVNQKVKSLSSVAEDLLLIELNGRESMRAGSV
                     LVTTNPQESLSFLEGLTSEVAIPKAESTSVGTVFFKINKTAVKNPPEGQGFVIPRKSS
                     YHTSKVVVLNNKWPLLEQAEHYYILVEFGRRLEETLIELADALVMEIIKNEVKEILTL
                     AEEPLQVIFYRWEKAVPHFSLQQRQEMLDDDYPVDREYAKRGVFVGGNGMTGYGMENA
                     IIEGKRLADEAIRYMKEMEKNNSPN"
     gene            4172..>4882
                     /locus_tag="PLT73_02175"
     CDS             4172..>4882
                     /locus_tag="PLT73_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM19220.1"
                     /translation="MELTILGCMGGYPTKDVGTTAYLLTSDDFKLLIDVGSNALLSLE
                     HHLDPLDLDAVILTHYHADHIADLGVLQYTFQLKEPAVGKSKKILPIYGHVESEFFRL
                     LEMPGVSEGIAYAPEETLELGPFRIRFLKTVHPVPCYALRIEEIATGKVFVFGADSAY
                     LPAFVPFVKDADLFLADANLFNGNERHHAHMTAGEVGTLAEEAAVKQLILTHLPQKGT
                     LSVLLDQAKEAAPSVSVTL"
     assembly_gap    4883..4892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4957..5964
                     /locus_tag="PLT73_02180"
     CDS             4957..5964
                     /locus_tag="PLT73_02180"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRM19221.1"
                     /translation="MYFVDNQGQLDPSVNIALETFLLKEKILDEPILLFYINEPSIII
                     GRNQNTIEEINADYVEANKIHIVRRMSGGGAVYHDLGNFSFCFITKDDGDSFRDFGKF
                     TKPVISFLHSVGVKEAELKGRNDLVIGEKKFSGNAMYATNGRMTAHGTILFDSDLDAV
                     TSALKPRKEKIESKGIKSIRSRVTNIKPFVDEAYQNLTTEQFRDLMLLSIFDVETREE
                     VKEYHLTDEDWKRVYEIREEYFGNWDWNYGKSPKFEIQKHHRFPIGSIEVRLNVEEGH
                     IQQLKIFGDFFGLGEIKDVEEAFIGVKYAKQDILDKLNELDIKKYFGNVTAEELLEEL
                     Y"
BASE COUNT         1813 a         1181 c         1488 g         1566 t
ORIGIN      
        1 caaatatcat ggaggtgaac gaccatagca aaagatatga tggtaaacga cggcatccgt
       61 gcccgcgaac tacgcatcat cggtagtgac ggcgagcaac taggagtcaa atcgaaaagt
      121 gatgcattgc agattgcaga agcagctaat ttagatttag ttttggtatc gccaaatgca
      181 aacccgcccg ttgcccgtat tatggattac ggcaagttcc gtttcgatca acaaaaacgt
      241 gaacgtgaag cccgtaaaaa ccaaaaagtg gtaagcctta aagaagtacg cttgagtcct
      301 tccatcgacg aaaatgattt tcaaacgaaa ttgcggaatg cacgcaaatt cttggaaaaa
      361 ggtgacaaag tgaaagcatc gattcgattc aaaggacgtg cgatcaccca caaagaaatc
      421 ggacaaaggg tccttgatcg ccttgcttct gagcttactg acgttgcgac gattgaatct
      481 catcctaaaa tggacggaag aagcatgttc ttgatgttgg cacccaaagc cgataaataa
      541 atttgtaact taatttagga ggaaagacaa atgccaaaac aaaaaactca ccgtggttca
      601 gctaaacgtt tcaaaagaac tggtaacggc ggactaaaac gttggagcgc ttttacaagc
      661 catagattcc acggaaaaac gaagaaacaa agacgtcaat tgcgtcaagc ttcaatggtt
      721 agcgcatctg atatcaaacg tatcagccaa caattatcac aaatgaaata attctaaata
      781 acaacaataa gagatagaac catggttcta ataaggagga aatcactatg ccacgtgtta
      841 aaggtgggac agtaacccgt aaacgccgca aaaagattat taaattatct aaaggttatt
      901 acggttcaaa acatacatta ttcaaaacag ctaaagaaca agtaatgaaa tcttatactt
      961 atgcttacag agaccgtcgt caaaagaaac gcgacttccg tagattatgg attgcccgta
     1021 tcaacgccgc tgctcgtatc aacggcatga gctacagcac tttgatccac ggattgaaac
     1081 ttgctggtat cgaaatgaac cgtaaaatgt tggctgattt ggctgttaca gacgctgcgg
     1141 cattcgctgc agtagctgag caagctaaag ctgctttggc taaataattt ttgaatcgct
     1201 gcctccccgg tcattgactg ggattgcggc gattttttgt ttttcttgca gtaaatgcta
     1261 tacttatttt atgtcactaa atcaatgggg gtatcatttt ggaagagaaa gaattacaaa
     1321 tgctgatcga tttgacggat gcgagaggcg ttccaggcaa tgaaggggag gtccgagaag
     1381 tttttagacg ctacgcggaa cctttcgccg aaagtttttc gcaggacgga ttgggcgggc
     1441 tctttgccaa acatacaggg gcggaagaag ggccaaccat tttattggcg gggcacttgg
     1501 atgaagtcgg gttcatggtc acaagtatca ccgagaaggg attcgtcaaa ttccaaacat
     1561 taggtggatg gtggaatcag gtcatgctgg cccaacaagt ggaaatcacc aactctaaag
     1621 gcgaacgttg ccacggagtc atcggttgta aaccgccaca tgtactgacg ccggaagcgc
     1681 gcaaaaaacc ttatgaaatt aaagatatgt tcatcgatat cggtgcttct tcgaaggagc
     1741 aagtagcgga atggggcatc aaacccggcg atatgatcac gccatacatt tctttcaaac
     1801 gcatgaacaa ttccaaattt cttttagcga aagcttggga taaccgcatc ggacttgccg
     1861 ttgcactgaa agtattggaa aatcttgcaa atactgcgca tccgaatata atttacagtg
     1921 gcggcaatgc tcaggaggaa gtggggctgc gtggtgcaaa gacagccaca catatgatcc
     1981 gtccggacat cgcctttgct ttggatacag gaactgctgg agatacgccg ggaatgacgc
     2041 ctcaagaagc ggattccgaa ttaggcaaag ggccgcaatt attgatttat gatgcatcga
     2101 tgatcccgca tcgtggttta cgcgatttta ttgttggtgt tgccgaagaa tgtgaaattc
     2161 cgttccagta cacggtcatc acaggtggcg gaacagatgc tggtgctcag catcagagcc
     2221 tggatggtat tccatctttt gctattactg ttccggtacg atacctgcat tcacacactt
     2281 ccatgatcca tgaggatgac tatttgaacg cagtcaaatt agtcactgaa atagtcaaac
     2341 gtttggatgc ggaaacagtc agacagatcc gcgaaaacgt ctgacaatat gcaaaacatg
     2401 gacatctgtc aaaaaatatc cacatatttt tgcggagtga tagagtaaga tatagatagt
     2461 gataaagtag gtgtcaagaa agcaagagga ggggatggaa ggatggagaa gttgaagcga
     2521 cgtatcgtaa tcatcggcgg tggacttaga ggattgacag ctgcttatga aatcgataaa
     2581 gccatccgga aacaaaacct tccgtttgag tatgtcgttc tggaggatcg atccgcagtg
     2641 ggcggtatga tccatacgat tgagatggac gggtatgcgt ttgatgtagg cgcatcagct
     2701 ttcgattcaa gacgaacgga tatttccggg ttttttcggg aattaggctt ggaaagcgaa
     2761 attcaattca gcaaaggtgg gaaactggtg ttgttcgatg ggaatgcaat catcgatgac
     2821 gttatgtcta cctaccacgg cataccactt tttccgaatg gtatttggca agccaatgga
     2881 ttgacctttg atgcaaagct ccgagcgttc atgaatagtc tgtttttgtc caaaaacttg
     2941 catgagaacc ctgagtattc aaccgacaac tttttggaat ttcgtttggg acgggaagtg
     3001 gtcgactata tggctgagcc ctattatcct gatagcgtct acggctctat ggaattaatg
     3061 ccggtaaaat tgttcgatga caatttggtg accattttcg gaagggcaca tatcggaaaa
     3121 aataaaaaag aacagttgtt gcgttttgcg gatggatccg gtcaggagta taccttcaaa
     3181 ggcggactga caaccttaat caaatctttg gcccagcact cggaaggcca tctgtatgtg
     3241 aatcagaaag tgaaatcatt gagcagtgtt gcggaagact tgctgctgat tgaattgaat
     3301 ggacgtgaat ccatgcgtgc cggaagtgtt ctcgtcacaa caaaccctca ggaatcctta
     3361 agttttttgg aaggtcttac cagtgaggtt gctatcccga aagccgaaag taccagtgtc
     3421 ggaaccgttt ttttcaaaat aaacaaaaca gcagtcaaaa atccgcctga gggacaagga
     3481 tttgtgattc cgcgaaaaag ttcctatcat acttccaagg ttgttgtctt gaacaacaaa
     3541 tggccgttgc ttgagcaggc tgaacactat tatatacttg tggaattcgg cagacgtttg
     3601 gaagaaacct tgatagaatt agctgatgct ctcgtgatgg agatcatcaa gaatgaagtg
     3661 aaggaaattt tgactttggc tgaagaacct ctacaggtca ttttttatcg ctgggaaaaa
     3721 gccgtaccgc atttcagtct gcagcaaaga caagagatgc tggatgatga ttatccagtc
     3781 gatagagagt atgcaaaaag aggcgttttt gtcggaggga acggaatgac gggttatgga
     3841 atggaaaatg ccatcataga gggaaaacgc ttggctgatg aggcgatccg ctacatgaaa
     3901 gaaatggaaa agaacaacag tccgaattaa aaatgaaaca gttcgcctat ctgtatgatt
     3961 acaaaggagg gtggactgtt ttttaaattc atgtagtgtt tatttttgtt tggtgctata
     4021 ataaattcaa tgatgtttaa ctgttaccta tttaccggaa aactgtttct ttctacaggg
     4081 gtgaaatgat tttcgattgt cgaaagctga aggcatgtta taatctgaat aagattcctt
     4141 gcttatcaga tattggattg gaggatgttt aatggaattg actattttgg gctgcatggg
     4201 tggctatcct acaaaagatg tcggtacgac agcttatctg ctgacatccg atgactttaa
     4261 actattgatc gatgtcggca gcaatgcttt attgtctctg gaacatcatc tggatccgtt
     4321 ggatttggat gcggtcattt tgacccatta tcacgcggat cacattgcgg atttgggtgt
     4381 tctgcagtac actttccagc tgaaagagcc tgcagtcggt aaatctaaaa aaatattgcc
     4441 gatatacggt catgtcgaat cggaattttt ccgcttgttg gagatgccgg gggtgagcga
     4501 agggattgcc tatgctccgg aagaaacgtt ggagttggga cctttccgga tccgtttttt
     4561 gaaaaccgtc catccggttc cgtgctatgc gttgcggatc gaggaaatcg caaccggaaa
     4621 agtcttcgtt ttcggggcgg attcagccta tttaccggca tttgtgcctt tcgtgaagga
     4681 tgctgacctg ttcttggctg atgccaatct gttcaacgga aacgagcgtc accatgcaca
     4741 tatgactgca ggcgaagtcg ggacgttagc tgaagaagcc gcggtcaaac aattgatcct
     4801 gactcatttg ccgcaaaaag gtacgctgtc tgtgctcctg gaccaagcga aagaggctgc
     4861 gccatccgtg tccgtgacgt tgnnnnnnnn nngaccggat tcagaatccg aaatgaaaca
     4921 acaaatcata atgaagaatg ggagaggaac aggcatatgt attttgtaga taatcaagga
     4981 cagttggatc ctagcgttaa tattgcgttg gagacatttt tattgaagga aaagatcttg
     5041 gacgagccga ttctgttgtt ctatataaat gaaccgtcga tcatcatcgg acgcaatcag
     5101 aatacgatcg aagaaatcaa tgctgactat gtcgaagcaa ataaaatcca tatcgtccgc
     5161 cgcatgtccg gagggggcgc agtataccac gacctaggta acttttcatt ctgcttcatc
     5221 acgaaagatg atggcgattc tttccgcgat ttcggaaaat tcactaagcc ggtcatttcc
     5281 tttttgcaca gcgtaggcgt caaggaagct gaattaaaag gaagaaacga cttggttatc
     5341 ggagaaaaaa aattctcagg gaatgccatg tacgcgacaa acggaagaat gacggcccat
     5401 gggacgatcc tattcgactc tgatttggat gccgtaacga gtgcgttgaa accgcgcaaa
     5461 gagaaaatcg aatcgaaagg gatcaagtcg atccgcagcc gcgtgaccaa catcaaacca
     5521 tttgttgatg aggcttatca aaatctgact acggaacaat tccgtgactt gatgctgctg
     5581 tcgatttttg atgtcgaaac gcgtgaagaa gtaaaagagt accacttgac cgatgaagat
     5641 tggaaacgcg tatatgaaat ccgggaagag tactttggca actgggattg gaattacggt
     5701 aaatcgccta aattcgaaat ccaaaaacat catcgcttcc ccatcggctc cattgaagtc
     5761 cgcttgaatg ttgaagaggg gcatatccaa cagttgaaga tttttggtga tttcttcgga
     5821 ttgggtgaaa tcaaagatgt tgaagaagcg tttattggcg tcaaatatgc caaacaggac
     5881 atcctggaca aactgaatga acttgacatc aagaaatact tcggcaatgt gacagccgaa
     5941 gagttgttag aagagttgta ctgataaaga aagagaggcc ccgcaatgtt tgcggagctt
     6001 ctcttttaga tctttatggc taaacgaacc cgttcagcat ttcttgtcca gcttcacg
//
LOCUS       DAOMUJ010000049         5970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3591_length_5970_cov_2.373288, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000049 DAOMUJ010000000
VERSION     DAOMUJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3591_length_5970_cov_2.373288"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1220
                     /gene="bsh"
                     /locus_tag="PLT73_02185"
     CDS             246..1220
                     /gene="bsh"
                     /locus_tag="PLT73_02185"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HRM19222.1"
                     /translation="MCTAVTYEGMNFYFGRNLDLDVSFNEKVTITPRQFEFRFRNEAA
                     IKSHWAMIGMAAVIDDYPLYYDAMNEKGLCMAGLNFPGNACYLSRKEGMENIAPFEFI
                     PFILGNCSSVEEAVAVLKNTILINEPFNDFLPLAPLHWIISDQKRSIVVEPMSHGLKI
                     FDNPVGVLTNNPTFDYHLTNLNNYLNLTSAPPNNRFSGKVDLSVSSIGMGSIGLPGDL
                     SSPSRFVRAAFTKANATEETDEGSEVAQFFHILDAVAFTKGCAVNEDGREELTIYSAC
                     MNTQKGIYYYKTYSNNQISAVYLNRADLDGSKLETFELITSQQIRDIN"
     gene            1435..1848
                     /locus_tag="PLT73_02190"
     CDS             1435..1848
                     /locus_tag="PLT73_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM19223.1"
                     /translation="MNLREISRLLYQIKLTNQEINTLFEKETGFSLTRYEMLMFLKDK
                     GTCSQNQIQTELKIDSAAITRHLKILEQKGYVIRERNAENNREVFVQLSDKAIKDLEA
                     CGKEHDQGKSSLSLSLSDEEAEQLSILLSKLYLQS"
     gene            1868..2506
                     /locus_tag="PLT73_02195"
     CDS             1868..2506
                     /locus_tag="PLT73_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM19224.1"
                     /translation="MTNKFINNNFSDIAFGRKSIRLYDENFKISQEEMLEMIQEATTT
                     PSSVNMQPWRFVVVESEEAKAKLKPLIRFNTRQNDTSSAMVLIFGDMQCYEYGEEIYD
                     TAVAQGKMSAEVRDQQLAAIIPYYKNFSKEQMNDVVKIDSSLAAMQFMLVARAHGYDT
                     NPIGGFEADQLADAFDLDKERYVPVMILSIGKAMEEGYESVRFAPEKITNFK"
     gene            2515..2802
                     /locus_tag="PLT73_02200"
     CDS             2515..2802
                     /locus_tag="PLT73_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRM19225.1"
                     /translation="MIVINAEFYIVPEQKKQFLNEISELIQASRKEEGCLSYQLYEAV
                     TDENNFVMVEKWENQQAIELHNATSHLQNFAKKVPDYSRDAPKITVSFVKE"
     gene            complement(2928..4124)
                     /locus_tag="PLT73_02205"
     CDS             complement(2928..4124)
                     /locus_tag="PLT73_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM19226.1"
                     /translation="MKLVGIVGSNAEVSYNRILLKYIKKQFSHLFDLEILEIKDIPLF
                     NQSDDQTNSAPVQKLTQKILQADGVIIATPEHNHTIPASLKSVLEWLSFKIHPLENKP
                     VMIVGASYYDQGSSRAQLHLRQILDAPGVNAIVLPGNEFLLGKVKEAFDEQENLKDKR
                     TIDFLGTCLEKFTQFITLVSAVEGPKKPVLEPEDLYATGKIATTIEGVDKEADDWLEQ
                     AAELVGAVEGNTYVKLERGLLTVDQLNYFLASMPMELTYADSNNQFLYYNHTKPAEEM
                     LAARVPGQVGNPLSKCHPERVHKGVEWVLQQLRSGTMDAFRVNVPTHGPDKYVVHNYQ
                     ALHDKDGNYAGVNEYILDFKPIIDWYLAQTGQKLIGDVDAVSSASVKDHHSDDVDAGT
                     SASVKA"
     gene            complement(4143..4751)
                     /locus_tag="PLT73_02210"
     CDS             complement(4143..4751)
                     /locus_tag="PLT73_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRM19227.1"
                     /translation="MNKLIGLVGTNSDHSTNRMLLQYIQKHFSDKADIEIVEIKDLPI
                     FNKPADMQIPAEAKALAEKIEAADGVIISTPEYDHAVPASLMSALSWLSYGIYPFVDK
                     PVMITGASYGVLGSSRAQAHLRQILDAPELKARIMPSSEFLLSHSLQAFDADNNLKNP
                     EQIQELEGLFEDFQMFINIMKQLSHTHAAHVKAAENFSWENN"
     gene            complement(4793..5749)
                     /locus_tag="PLT73_02215"
     CDS             complement(4793..5749)
                     /locus_tag="PLT73_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM19228.1"
                     /translation="MEQKKATVRLMGTIIDLLVTHTHPDAILEEAIRRLRIYEQRFSA
                     NDPKSELMQINHLAGKAPLAVHPDLFHLIKVGKYHSLAPNSRLNIAIGPLVQTWRIGF
                     GDAKVPDAEEIQRLLEITDPNKILLSEKDQTVFLQQEGMSIDLGALAKGYIADLLTDY
                     FKKAGVASALINLGGNVVVFGPSQNNEDPYWRIGIQNPMLPRAHYSAVLKIQNQSAVT
                     SGVYERQLEAQGKHYHHILDPQTGYPAETDVISLTVVADDSVDGEIWTTRLFGQPADV
                     ILAALDHSEAIDGILITKDGSLYCSKGLENRLQRKQLSQSRV"
     gene            complement(5774..>5970)
                     /locus_tag="PLT73_02220"
     CDS             complement(5774..>5970)
                     /locus_tag="PLT73_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM19229.1"
                     /translation="VTYYLARAAIQLAKNAIIQGKPQQEVLELIHDARAYSKINKNTI
                     ALKELAQLESAILSGNPATE"
BASE COUNT         1629 a         1238 c         1421 g         1682 t
ORIGIN      
        1 ttcctctaca ctaaattggc taccaacctt tagttgtatt gtaaggtctt ctttttgact
       61 atttcaacaa aagaatagtt ttgcatagaa aaattataca cttaggaatt aagtgtaaga
      121 tcatttgaaa aagagaaaac actctcgaga gctttgtgat tgcacctcac caaatgtagg
      181 aatatagtaa gggaaacaat acaaaagaat agcctgtatt ctgcaaagga ctggaggaat
      241 tttttatgtg tacggcagtg acatatgaag ggatgaattt ttattttggc agaaatctgg
      301 acttagatgt ttcgttcaac gagaaagtaa cgattactcc acggcagttt gaatttcggt
      361 tcaggaatga agcagcaatc aagtctcact gggcgatgat cgggatggct gcggtaatcg
      421 atgattatcc gctgtattat gatgcaatga atgaaaaagg gttatgcatg gcgggactaa
      481 attttcctgg gaatgcctgt tatttgtcca gaaaagaagg aatggaaaat attgctcctt
      541 tcgaattcat tccctttatt ctcggcaact gcagcagtgt ggaagaagcc gtggctgtat
      601 tgaaaaatac gattctgata aatgaaccat tcaatgattt tctgcccttg gcaccgctgc
      661 attggatcat ctcagatcag aagcgctcga ttgttgtgga acctatgtcg catggattga
      721 agatatttga taatccagtg ggtgtcctga cgaataaccc aacgtttgat tatcatttga
      781 cgaatctgaa taattacctg aatcttacat cagcccctcc gaacaatcga ttctccggaa
      841 aagtggattt gtcggtatct agcatcggta tgggcagtat cggcttacca ggagatcttt
      901 cttctccatc gcgatttgtg cgtgctgctt ttacgaaagc gaatgcgacg gaagaaacag
      961 atgaaggctc agaggtggct caatttttcc atattttgga tgctgtggcc tttacaaaag
     1021 ggtgtgccgt caacgaagat ggcagagaag aactaacgat ctattcggca tgcatgaata
     1081 cccaaaaagg catttattat tacaaaactt acagtaacaa tcaaatcagt gcagtctatt
     1141 tgaatcgtgc agatttggac ggaagtaagc ttgaaacctt tgaattgatt acttctcagc
     1201 aaatccgaga catcaattaa tagctgtaac ggttcaaatt agaaatcgat tgatcagtcc
     1261 atctattttc attgcaaggc cttgtcccaa agacaaggct ctttttggat agcgggtaat
     1321 tttaagcaag ttactttaca aaagttagtt cctgcgttat gattgacagg tcaacagttg
     1381 acaagtcaat tgtttgatca gtcaggataa tccgcaaaat cgaggtgaat tggtatgaac
     1441 ttgagagaaa tcagcaggct gctttatcag atcaagctca caaatcagga aatcaatacg
     1501 ctgttcgaaa aagaaacagg tttcagcttg acgcgctatg agatgctgat gttcctgaaa
     1561 gacaagggga cgtgttccca aaatcagatt cagacagaac tgaagataga cagcgcggct
     1621 attacacgcc atctgaaaat attggaacaa aagggctatg tcatccggga acggaatgct
     1681 gagaataacc gagaggtctt tgttcaatta tccgacaagg caatcaaaga tttggaggcg
     1741 tgcggaaaag aacatgatca ggggaaatct tccctatcgc tttcattaag cgacgaggaa
     1801 gcagagcaat tatctatttt gctgagtaaa ctatacttac aaagttgaaa aggaagaggc
     1861 tataaacatg accaataaat ttatcaacaa caatttttcg gatatcgcat tcggaagaaa
     1921 atcgatccgt ctttatgatg aaaattttaa aatcagtcaa gaagaaatgc tggaaatgat
     1981 tcaggaagct acgactacac cttcatccgt gaatatgcaa ccttggcgtt ttgtggttgt
     2041 tgagagtgag gaagcaaaag ccaaattgaa accgctgatc cgtttcaata ccagacaaaa
     2101 tgatacttcg tcagcaatgg ttttgatatt cggcgatatg caatgctacg agtacggaga
     2161 agaaatctac gatacagctg tcgcacaagg caaaatgtcc gccgaagtcc gagaccaaca
     2221 attagctgca atcattcctt actacaagaa cttttctaaa gagcagatga atgatgttgt
     2281 caaaatcgat tcgagtttgg cagcgatgca attcatgctg gttgcccgcg cccatggcta
     2341 tgacacgaac cctatcggcg gctttgaagc agatcagttg gcggatgcgt tcgatctcga
     2401 caaggaacgg tatgtgccgg ttatgatctt gtcaatcggt aaagcaatgg aagaaggcta
     2461 tgaatccgta cggtttgcgc cagagaaaat cactaatttt aagtaggtgg agaaatgata
     2521 gtaatcaatg cagaatttta tattgtacct gagcaaaaaa aacaattttt aaatgaaatt
     2581 tcggaattaa ttcaggcatc cagaaaagaa gaaggttgct tgtcttatca actttacgaa
     2641 gcagttacag atgaaaataa ttttgtaatg gttgaaaaat gggaaaatca acaagcaatt
     2701 gagttacata acgcaacgtc tcatttgcaa aacttcgcta agaaagttcc ggattatagt
     2761 cgcgatgcac ctaaaataac ggttagtttt gtaaaagagt aataaaatat cgttccgtta
     2821 aaatagttat actaaactct atccaaaaag acctcattac tttatagtat gaggtctttt
     2881 tgatataggt tttgcttttg gagatgaaaa gcatctctgc gcagtgctta tgccttaacc
     2941 gacgcgcttg taccagcatc cacatcatcg ctgtggtggt ctttgacgga agcgctggaa
     3001 acggcatcga catcgccaat cagtttttgg ccggtttggg ccagatacca gtcaatgatc
     3061 ggtttgaagt cgaggatata ttcgttgacg ccggcatagt tgccgtcttt gtcatgcagc
     3121 gcttgataat tatgcacgac atacttgtcc ggtccatgcg tcggaacgtt gacacggaag
     3181 gcatccattg tgccggaacg caattgttga agtacccatt ccactccttt atgcactctt
     3241 tccgggtggc atttggaaag cgggttgccg acttggccag gtacacgcgc agcgagcatt
     3301 tcttcggcag gtttcgtatg gttgtagtaa aggaattggt tgttgctgtc tgcatacgtc
     3361 aattccatcg gcatcgacgc caggaagtaa ttcagctgat caacagtcaa caagccgcgt
     3421 tccagtttga cgtaggtatt gccttccaca gcgcccacaa gctcagccgc ttgttccaac
     3481 cagtcatcgg cttccttatc gacgccttca atcgttgttg cgatttttcc ggtagcgtat
     3541 aggtcttccg gttcgagcac cggttttttc ggtccttcaa cagcagagac caacgtgatg
     3601 aactgagtga atttttccag acaagtgccc agaaaatcga tggtgcgctt gtctttcaga
     3661 ttttcctgtt catcgaaggc ttctttgact tttcccagca aaaattcatt acccggaagg
     3721 acgatggcat tgacgcctgg tgcatcgaga atttgacgca ggtgcagttg ggcccgggaa
     3781 gatccttgat catagtagga agctcccaca atcatgaccg gtttgttttc caatggatgg
     3841 attttgaagg acaaccattc cagtacactc ttcaagctgg caggaattgt atggttatgt
     3901 tcaggagtgg caatgatgac gccatccgcc tgcaggattt tttgggtcag tttttggaca
     3961 ggggcgctgt ttgtttggtc atcactctga ttgaacaaag ggatgtcctt aatttccaaa
     4021 atctccaggt cgaacaagtg gctgaactgc tttttgatgt atttcaacaa gatacggtta
     4081 tatgatactt ccgcgttgga acctactata ccgactaatt tcataatgtt tgtctccgat
     4141 ctctagttat tttcccaaga aaagttttca gctgctttta cgtgtgcagc gtgggtgtgc
     4201 gatagttgtt tcatgatgtt gatgaacatt tggaagtcct cgaataagcc ttccagctcc
     4261 tggatctgtt ctggattttt caggttgttg tctgcgtcga aagcctgcaa agaatggcta
     4321 agcaggaact cagaacttgg catgatgcgg gctttcaatt ccggcgcatc caagatctga
     4381 cgtaaatgcg cttgcgcccg tgacgaaccc agtacgccat aggaagcccc tgtgatcatg
     4441 acgggcttgt cgacgaaggg gtagatgccg taggataacc agctcaaagc gctcatcagg
     4501 gaagcaggaa ctgcgtgatc gtattctggt gtgctgatga tgacgccgtc agctgcttcg
     4561 atcttttccg ccaaggcttt cgcctcagcc gggatctgca tgtctgccgg tttgttgaag
     4621 atcggcaggt ccttgatttc gacaatctcg atgtctgcct tatcggaaaa atgcttttgg
     4681 atgtattgca gcagcatacg gttagtggag tggtccgagt ttgtgccgac gaggccgatt
     4741 aatttattca tgtgaatact tccttccaaa ttatagtttt tttatgattc atttaaacac
     4801 gtgattgaga aagttgtttg cgttgcagcc ggttttccaa gcctttggag cagtagaggc
     4861 taccgtcttt cgtgatcagg atgccgtcga tggcttccga gtgatctaat gcggctagga
     4921 tcacgtcggc aggttggccg aataaacgcg tcgtccagat ttccccatcg acagagtcat
     4981 cggcaaccac agtcaagctg atgacatccg tttctgccgg atagccggtc tgtggatcca
     5041 aaatatggtg atagtgcttg ccttgggctt ccagctgccg ttcatacaca ccagaggtaa
     5101 ccgctgattg gttctggatt ttcaataccg cgctataatg cgcgcggggg agcatcggat
     5161 tttggatgcc gatgcgccaa tagggatctt cattgttttg ggatggtccg aacacaacga
     5221 catttccacc caaattgatg agagctgagg ctacaccggc tttcttgaag tagtccgtca
     5281 ggagatcggc aatatagcct tttgccaaag cccccaagtc tatggacata ccttcttgct
     5341 gaaggaaaac ggtctgatcc ttctcggaca agaggatttt attgggatcg gtgatttcaa
     5401 gaagccgctg aatctcttcg gcatccggga ctttggcatc accgaaaccg atcctccaag
     5461 tctgcaccag cggtccgatg gcgatattca gacgactgtt gggggctaag ctatggtatt
     5521 tacccacctt gatcaagtgg aacaaatcag gatggacagc aagaggagct ttgcctgcca
     5581 aatggttgat ctgcatcagc tccgactttg gatcattggc gctgaaacgt tgttcgtata
     5641 ttcgtaggcg tcggatcgcc tcctccaaaa tagcgtccgg gtgcgtgtgc gtcacaagca
     5701 gatcgatgat tgttcccatc agacggaccg ttgctttttt ttgttccata ttttccacca
     5761 caggataacc gtgtcattct gttgcgggat tccccgaaag gatcgcgctt tccaactgcg
     5821 ccaattcttt cagcgcaatc gtgtttttgt tgatttttga ataggctctg gcatcatgga
     5881 tcaattccag cacttcttgc tgcggcttcc cctggatgat ggcatttttg gccaactgga
     5941 tggctgcacg ggcaagatag taggtcacgt
//
LOCUS       DAOMUJ010000050         5959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3600_length_5959_cov_2.283875, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000050 DAOMUJ010000000
VERSION     DAOMUJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_5959_cov_2.283875"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLT73_02225"
     CDS             <1..790
                     /locus_tag="PLT73_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HRM19230.1"
                     /translation="IIDEPLTVSIHFRGPAKRIKGEILFKYGETVFNQNRAAKTASQF
                     LIRDTIAEERILQVLEALEFNIIENQISFLAAKDIDLFSFLYRKVPTLRRYAEVELSP
                     DLQNLVVEDTVVSTSVQRRTRDSFLDIRFDVDGISDNEIDKLLNSLKKNDAYYKTEDG
                     RILSLETETMREMNRFLSLIKDESQVREGTLSMPMIKSLTLEKEINDLNPEVGSQAFL
                     NELYHFIKHPDEFPAELPKSLQADLRPYQMAKVPCQLWFGWYFG"
     gene            783..>2032
                     /locus_tag="PLT73_02230"
     CDS             783..>2032
                     /locus_tag="PLT73_02230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM19231.1"
                     /translation="MADEMGLGKTLQLITFLLSEVEEGKNENKPALVIAPASLIYNWK
                     YEFQKFAPLLQISVISGVKKEREEQLSQLPESGIAITSYQSFRQDIESYQKCDFYAMI
                     LDESQYVNNYNTKTFRAIAKMNARVNIALSGTPIENSVTEFWAIFQLILPGLFPKLQE
                     FKKMPLEDISKTAKPFMLRRLKADVLGDLPEKSEADIYSSLNREQKVMYLAYLQQLQE
                     KVSDYDSEDFQRNRMEILAGLTRLRQICCDPRLVDPVYEGGSGKLEQLVEILRNAQEN
                     NQHVLVFSQFTSMLKLIEAELAKENLSHLYLSGKTPPAERITLVNQFNEGYTDVFLIS
                     LKAGGTGLNLTGADTVILFDLWWNPAVEEQAAGRAHRIGQKKNVQVYRFIAEGTIEEK
                     INQLQQDKKMLFDQLVVSEGEEAL"
     assembly_gap    2033..2042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2266..>2876
                     /gene="rpsB"
                     /locus_tag="PLT73_02235"
     CDS             2266..>2876
                     /gene="rpsB"
                     /locus_tag="PLT73_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRM19232.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKRYIFTERNGIYIIDLQ
                     KTVKLVDEAYKYMLNVAEEGGVALFVGTKKQAQDSIKDEAIRSGQFFVNHRWLGGTLT
                     NWDTIQKRIKRLKSIEAMSEDGTFELLPKKEVSILKKEQERLEKFLGGIKDMPRIPDV
                     MFIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSN"
     assembly_gap    2877..2886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2887..3082
                     /locus_tag="PLT73_02240"
     CDS             <2887..3082
                     /locus_tag="PLT73_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRM19233.1"
                     /translation="VIIPANDDAIRAVKLITAKMADAFIEGNQGNDQAEISVEETFAA
                     EADEATSLEEIVEVVEGDNE"
     gene            3218..4099
                     /gene="tsf"
                     /locus_tag="PLT73_02245"
     CDS             3218..4099
                     /gene="tsf"
                     /locus_tag="PLT73_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRM19234.1"
                     /translation="MAQVTAKMVKELRDMTGVGMMDAKRALVEVDGDMDKAVDYLREN
                     GMAKAAKKADRIAAEGLANVYVDGNTAVIAEINAETDFVAKNDKFQNLVADITAIIAK
                     NKPETLEAALAIETADGSLNDVILNATTVIGEKITLRRFAIVEKTDADAFGAYLHQGG
                     RIAVLTVLEGSTDADAAKDISMHIAAINPKYVSRDEVSAEELEHEKKVLTEQALNEGK
                     PANIVEKMIVGRLNKFLSEISLNDQPFVKDPDQTVSQFAASKGGVVKSFVRYAVGEGI
                     EKRSVDFAEEVMSQVKK"
     gene            4187..4909
                     /gene="pyrH"
                     /locus_tag="PLT73_02250"
     CDS             4187..4909
                     /gene="pyrH"
                     /locus_tag="PLT73_02250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRM19235.1"
                     /translation="MSEPKYKRVVLKLSGEALAGDTGFGINPPTIEEIVKEIKEVHDL
                     GVEIAIVVGGGNIWRGTTGAEMGMERAQADYMGMLATVMNSLALQDALENEGVPTRVQ
                     TSIEMRQIAEPYIRRRAVRHLEKGRVVIFAAGTGNPYFSTDTTAALRAAEINADVILM
                     AKNNVDGVYSSDPMKDLNATKFSELTHLDVISKGLQVMDTTASSLSMDNDIPLVVFNL
                     NRQGNIRRVIMGEDIGTTVRGK"
     gene            4912..5469
                     /gene="frr"
                     /locus_tag="PLT73_02255"
     CDS             4912..5469
                     /gene="frr"
                     /locus_tag="PLT73_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016626710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRM19236.1"
                     /translation="MVKEILDNAIDRMKKTEAALIRELGSIRAGRANASLLDRIEVEY
                     YGTSTPVNQLASITIPEGRMLLVTPYDKSSIGDIERAIYQSDLGINPANDGNVIRLVI
                     PALTSERRKELAKTVGKENEAAKISIRNIRRDAIEALKKAEKNKEITEDELRTYEKKV
                     QEFTDKSSKEIDKITADKEKEILDV"
     gene            5625..>5959
                     /gene="uppS"
                     /locus_tag="PLT73_02260"
     CDS             5625..>5959
                     /gene="uppS"
                     /locus_tag="PLT73_02260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRM19237.1"
                     /translation="MALFKSENPKTDLQFDENGIIPAHVAIIMDGNGRWAKKKLMPRV
                     YGHKEGMNTVKKITIEASRLNVKVLTLYAFSTENWKRPTDEVNFLMGLPIDFFDVFMP
                     ELMQNNIKVT"
BASE COUNT         1923 a         1165 c         1374 g         1477 t
ORIGIN      
        1 catcattgat gaaccattga ctgtctcaat ccatttccgc ggcccggcaa aaaggataaa
       61 aggggaaatt ttattcaaat acggagaaac agtcttcaat caaaaccgcg ccgccaaaac
      121 ggcttcccaa ttcttgatca gagacacaat agcggaagag agaatcctgc aggtgttgga
      181 agctttggaa ttcaacatca tcgaaaacca aatatctttc ctggctgcaa aagatatcga
      241 cctattcagc tttctttaca gaaaagtacc aacattgcgc cgctatgctg aagtggaatt
      301 aagtccggat ctgcagaacc tggtagtcga ggacacagtc gtgtcaactt cagtccaacg
      361 cagaacaagg gattcttttt tggatattcg ttttgatgtt gacggaattt cggataatga
      421 aatagacaaa ttactgaaca gtttaaaaaa aaatgacgcc tattacaaga cggaggacgg
      481 gcgaatattg tctttggaga cggaaacaat gagggagatg aatcgcttcc tttcgttgat
      541 caaagatgag tcacaagttc gtgaggggac tctgtccatg ccgatgataa aaagcctgac
      601 gcttgaaaaa gaaatcaatg acttgaaccc tgaggtaggc agtcaagctt tccttaatga
      661 actttatcat ttcatcaagc acccggatga gtttcccgct gaactaccga aatcgttgca
      721 agcggatttg cgtccctatc aaatggctaa agttccttgc caactatggt ttgggtggta
      781 ttttggctga tgaaatggga ctcggtaaga cgctacagct gatcaccttc ttgctcagtg
      841 aagtagaaga aggcaaaaac gaaaataaac ctgctctcgt gatcgctccg gcttcgctga
      901 tatacaactg gaagtacgaa ttccagaaat tcgcaccttt gcttcagatc agcgttatca
      961 gcggcgtgaa aaaggaacgg gaagagcaat tgtcccaact gccggagagc ggtatcgcaa
     1021 tcacatctta tcaaagtttc cgtcaggaca tcgaatctta tcaaaagtgt gatttctatg
     1081 cgatgattct ggatgaatcc cagtacgtca ataactataa caccaagacg ttccgtgcca
     1141 ttgcgaaaat gaacgcccga gtcaacatag cattgagcgg tacgccgatc gagaacagcg
     1201 taacggagtt ttgggcgata ttccaattga tcctgcctgg gttatttcca aaattgcagg
     1261 aattcaaaaa aatgccactc gaagatattt cgaaaacagc caaaccattt atgttgcggc
     1321 ggttgaaggc ggatgtgttg ggcgacctgc cggagaaatc ggaagcggac atatacagca
     1381 gtctaaaccg tgaacagaaa gtcatgtatt tggcttatct gcagcaactg caggaaaagg
     1441 tatctgatta tgacagtgag gatttccaac ggaaccggat ggaaatcttg gccgggttga
     1501 ctcgtttgcg tcaaatttgc tgtgatcccc gtttggtgga tcctgtttat gagggaggat
     1561 ccggaaaatt ggagcaactg gtcgaaatat tgaggaatgc tcaagaaaac aaccagcatg
     1621 ttttggtttt ttcacagttc acttccatgc tcaagttgat tgaagcggaa ttggccaagg
     1681 aaaacttaag tcatctgtac ctcagcggaa aaaccccacc tgcagaaaga attacgctcg
     1741 taaatcagtt caacgaaggc tatacggacg tctttttgat ttcactgaag gcgggcggca
     1801 ccgggctgaa cctgactggc gctgacactg tcatcctttt cgatctgtgg tggaacccgg
     1861 cagtcgaaga gcaagccgca ggcagagcgc atcgcatcgg acaaaagaaa aacgtgcaag
     1921 tgtatcgttt cattgcagag ggaaccatcg aagagaaaat caatcaactc cagcaagata
     1981 aaaaaatgct ctttgatcag ttggtcgtat cggaagggga agaagcgctc aannnnnnnn
     2041 nntacaaacc tgatatatcc tataaaaaag tttccaaaaa gtttctgtta ttcattttca
     2101 gaaaaaagac ttgcttcttt cagcgtaatt cgatataatt atcaacggtg caaaaccaca
     2161 tttcacacct tgatcgatac ggcggacgtt gcttatcgta agatgagttt ctgctgtaga
     2221 agaaatcggg ggaggaaaat aaaaacaaac ttggaggaaa taaacatggc agtaatttca
     2281 atgaaacaat tattggaagc aggcgtacac ttcggtcacc agactcgtcg ttggaaccca
     2341 aaaatgaaga gatacatctt tacagagaga aacggtatct acatcatcga cttgcaaaaa
     2401 accgtaaaat tagtagacga agcatacaaa tatatgctta atgttgctga agaaggcggc
     2461 gtagctttat tcgtagggac taaaaaacaa gcgcaagatt caattaaaga cgaagctatc
     2521 cgttcaggtc agttcttcgt aaatcaccgt tggttaggtg gtactttgac taactgggat
     2581 actatccaaa aacgtatcaa acgtttgaaa tcaatcgaag ctatgtctga agacggcact
     2641 ttcgaactac ttcctaaaaa agaagtttca atcttgaaaa aagaacaaga acgtttggaa
     2701 aaattcttgg gcggtatcaa agatatgcct agaatcccgg atgtaatgtt catcgttgac
     2761 ccaagaaaag aaagaatcgc tgttcaagaa gcacacaaat tgaacattcc tatcgtcgct
     2821 atggttgaca ctaactgtga tccagatgaa atcgacgtag taatcccttc aaatgannnn
     2881 nnnnnntgtt atcatcccag cgaacgacga tgcaatccgt gcagttaaat tgatcactgc
     2941 taaaatggct gatgctttca tcgaaggcaa ccaaggaaat gatcaagctg aaatttctgt
     3001 tgaagaaact tttgcggctg aagctgacga agcaacttca ttagaagaaa tcgttgaagt
     3061 cgttgaaggc gacaacgaat aatatttcct atttagtttg aagcaatgag ctgtctcaga
     3121 aaagaagaaa gatatagcca attaggtagc cgacatcttt tctatctctt tggggcagct
     3181 ttttttatga aaaagaatat tcaggaggaa tcataaaatg gcacaagtaa cagcaaaaat
     3241 ggttaaagaa ttacgcgaca tgacaggcgt aggcatgatg gatgctaaaa gagctctagt
     3301 agaagtcgat ggcgatatgg acaaagcggt tgactaccta agagaaaatg gtatggcaaa
     3361 agcagccaaa aaagctgacc gtatcgctgc tgaaggactt gcaaacgtat atgttgacgg
     3421 aaatacagct gttatcgctg aaatcaatgc cgaaactgac tttgttgcga aaaacgacaa
     3481 attccaaaac ttggtagctg atattacagc aatcatcgct aaaaacaaac cagaaacgtt
     3541 ggaagctgct ttagcgatcg aaactgcaga cggctcattg aacgatgtca tcttgaatgc
     3601 tactactgta atcggcgaaa aaattacttt gcgtcgcttt gctatcgttg aaaaaacaga
     3661 tgctgatgct ttcggcgcat acctgcacca aggcggacgt atcgctgtat tgacagtttt
     3721 ggaaggctct acggatgctg atgctgctaa agatatttct atgcacatcg ctgccatcaa
     3781 tcctaaatat gtttctcgcg acgaagtttc cgcagaagaa cttgagcatg aaaagaaagt
     3841 tctgactgaa caagctttga acgaaggcaa accagctaac atcgttgaaa aaatgatcgt
     3901 aggccgtttg aacaaattct tgagcgaaat ttcattgaac gaccaaccat tcgttaaaga
     3961 tccagatcaa acagtttcac aatttgctgc ttcaaaaggc ggcgttgtta aatcattcgt
     4021 tcgctacgct gtaggcgaag gtatcgagaa acgctctgtg gactttgcag aagaagtaat
     4081 gagccaagtt aaaaaataat gaaaattgcc ccttaattgg ggctttttta tcatagtcat
     4141 aagcctgatt aatcaggggt cagccaatta cggagggaaa tatagaatga gtgaaccaaa
     4201 atacaaacgc gttgttttaa aactaagtgg tgaagcttta gcgggcgaca ccggtttcgg
     4261 aattaaccca ccgacgattg aagaaatcgt aaaagaaatc aaagaagtac atgacttggg
     4321 tgtggagata gctatcgttg tgggcggcgg caatatttgg cgcggcacga caggcgctga
     4381 aatgggtatg gagagagcgc aagccgacta tatgggcatg cttgcgactg tcatgaattc
     4441 tctggctctt caggatgcct tggagaatga aggggttcct acccgtgttc agacttccat
     4501 cgaaatgaga caaattgccg aaccgtatat ccgcagaaga gctgtccgtc accttgaaaa
     4561 aggacgcgta gtgatatttg ctgccggtac cggcaatcca tacttctcta ctgacacgac
     4621 agcagccctt cgtgccgctg aaatcaatgc tgatgttatt ttgatggcta agaacaatgt
     4681 tgatggggtc tattcatccg atccgatgaa ggacttgaat gcaactaaat tctctgaatt
     4741 gactcatttg gatgttattt ccaaaggatt gcaagtaatg gatactacag caagctcatt
     4801 aagcatggat aacgacatcc cgctggttgt atttaactta aacagacaag ggaatattcg
     4861 ccgtgttata atgggagaag atatcggaac gactgttagg gggaaataat tatggttaaa
     4921 gaaatccttg ataatgcgat cgacagaatg aaaaaaacgg aagctgcact gatccgcgaa
     4981 ctaggctcga ttcgagcagg ccgcgcgaat gcgagtttat tggatcgtat cgaagttgaa
     5041 tactacggaa cgagcacacc agtgaatcaa ttagcatcca tcacgattcc tgaaggacgc
     5101 atgttgttgg tcacacctta cgacaaatcc agtatcggtg atatcgaacg cgcgatttac
     5161 caaagcgact tgggcatcaa cccagccaat gacggcaacg tcattcgttt ggtgattccg
     5221 gcattgactt cagagcgtcg taaagagttg gcaaaaactg ttggcaaaga aaacgaggca
     5281 gctaagattt ctatccgcaa tattcgccgt gatgccatcg aggcgttgaa aaaagcagaa
     5341 aagaacaaag aaatcacaga agacgagtta cggacttatg aaaagaaagt acaagagttc
     5401 accgacaaat cttccaaaga aatagacaaa ataacggcgg acaaagaaaa agaaattttg
     5461 gacgtttaac attccattcg ttggaaagca gccagggcac aaatcatttc gaatgatttg
     5521 tgccctggct ctttttaaaa attatataaa ttccggttaa tcataggaat tcaggcgggt
     5581 ttttgttaaa ataaaaagag ttatgaaaaa gtgagggata aagcatggct ctattcaaga
     5641 gtgagaatcc aaaaacagat ttgcagtttg acgaaaatgg catcattcca gctcatgttg
     5701 ccatcattat ggatgggaac ggccgttggg caaagaaaaa actgatgcca cgggtttacg
     5761 gacacaaaga gggtatgaat acggttaaaa agataaccat agaggcaagc cgccttaatg
     5821 tgaaggtatt gacgctgtat gccttttcga ctgaaaactg gaaacgtccg actgacgaag
     5881 tcaatttttt gatgggattg cccatcgatt tttttgatgt ctttatgccg gagttgatgc
     5941 agaacaacat caaagtgac
//
LOCUS       DAOMUJ010000051         5896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3662_length_5896_cov_2.527821, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000051 DAOMUJ010000000
VERSION     DAOMUJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_5896_cov_2.527821"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..567)
                     /locus_tag="PLT73_02265"
     CDS             complement(52..567)
                     /locus_tag="PLT73_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRM19238.1"
                     /translation="MKKEILIVDGYNMIGAWPELVKLKNQDLIEEARDQLLQALSDYQ
                     NYEGNEVWCVFDAQFVPGITKEYSKYRVKVIFTAQGETADTYIEGVVKKLRNVLTEVY
                     VATSDLAEQQLVFSKGAQRISAMELYKDIKRSKKALETESRRFRDQRQRGTWSDDQLE
                     VLREIYKDMLE"
     gene            complement(616..1332)
                     /gene="rlmB"
                     /locus_tag="PLT73_02270"
     CDS             complement(616..1332)
                     /gene="rlmB"
                     /locus_tag="PLT73_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRM19239.1"
                     /translation="MGRHPVLELLRSDRDVNKLFIQDGLGGEKLGDIIKLAKDRKIQI
                     QAVPKSKLDTLSDNAVHQGIIAATAAFQYAVLDDLFEAAAAKNEDPFFIILDGVEDPH
                     NLGSVLRTADACGAHGVIIPKRRAVGLTATVAKASTGAIEHVPVVRVTNLHRTIEELK
                     ERGVWIYATDMSGQDYRQWDTTLPLAMIIGNEGKGISRLLKESADGLLTIPMVGHVQS
                     LNAGVAAGLMMYEVFRKRHV"
     gene            complement(1448..1873)
                     /locus_tag="PLT73_02275"
     CDS             complement(1448..1873)
                     /locus_tag="PLT73_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HRM19240.1"
                     /translation="MEKSIVTEQNWSLLNGLALAYVGDAAYETYIRTHLLEKGHTKPN
                     QLHRRATFFVSAKAQAKLMHAMLEKEGFLSEAEMDMYRRGRNSKSHTIAKNADVTTYR
                     IATGFESLMGYLHLSGQKVRLEELIRWCIQEVEENNYEK"
     gene            complement(1858..3270)
                     /gene="cysS"
                     /locus_tag="PLT73_02280"
     CDS             complement(1858..3270)
                     /gene="cysS"
                     /locus_tag="PLT73_02280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM19241.1"
                     /translation="MLKIYNTLTREKEEFQPIQKNKVGMYVCGPTVYNYIHIGNARST
                     VAFDTVRRYLEYRGYDVNFVSNFTDVDDKIIRAAKELGISAKEVAEKFIAAFYEDTAA
                     LHVEKATLNPRVMENIPDIIAFIGDLIDKGYAYEADGDVYYRTGKFKDYGKLSDQSIK
                     DLIVGASERLDAEENVKKEDQLDFALWKAAKPDEVSWESPWGPGRPGWHIECSVMATK
                     YLGDTIDIHGGGHDLTFPHHENEIAQSEAKTGKTFANYWMHNGFVTIGETAEKMSKSL
                     GNFVTVHDILKEVDPQVLRFFLGTAHYRRPVKYSEKAIEEAKINLEKVKIAYQNAHYR
                     LQDAVAALPDDAEQLALFQNLQAQFVAEMDDDFQADNAMSIVYQFAKELNIYAEGEAV
                     SEAVITAALALYKAMVGVFGVVLGEEALLDDDIQTLIDERTQARTDKNFARSDEIRDY
                     LKDQGIILDDTAQGTRWRRA"
     gene            complement(3326..3865)
                     /gene="cysE"
                     /locus_tag="PLT73_02285"
     CDS             complement(3326..3865)
                     /gene="cysE"
                     /locus_tag="PLT73_02285"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRM19242.1"
                     /translation="MNRLRETIQTIKDHDPAARSTLEVVLTYPGLHAILIYDIAHFFY
                     KRKWYTFSRVLSHLGRFLTGIEIHPGATIGHRLFIDHGMGIVIGETAEVGDDVMIYHG
                     VTLGGTGKDTGKRHPTIGNNVLLSAHVQVLGPIKIGDNAKIGASAVVVSEIPPDVTAV
                     GIPAKIVRYHKRSEEKMTE"
     gene            complement(3944..5413)
                     /gene="gltX"
                     /locus_tag="PLT73_02290"
     CDS             complement(3944..5413)
                     /gene="gltX"
                     /locus_tag="PLT73_02290"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRM19243.1"
                     /translation="MTKKIRVRYAPSPTGNLHIGNARTALFNYLFARHNDGEFIIRIE
                     DTDQKRNLEHGEESQLQNLKWLGMDWDEGPDKPGKFGPYRQSERQHIYDPLIDQLLLS
                     NRAYKCYCTEDELETEREHQRARGEMPHYGGKCANLTPSQQAEKEAQGITPVIRFRVP
                     IENTYTFDDIVKGPITFEASSVGGDYIIRKRDGFPTYNFAVAVDDHMMEITHVLRGDD
                     HIANTPKQMMIYEAFEWKIPTFGHMTLIINSETGKKLSKRDETILQFIEQYKDLGYLP
                     EAMFNFITLLGWSPVGEDEIFSKDELIKMFDPARLSKSPAAFDQKKLEWINNQYMKSA
                     DLDTIVTLAAPHLVAAGKLPENPSEADSEYAKKLIGLYHEQMSYAAEIVNLSEMFFAD
                     ELSLDAEAQEILAAETAPVVLAAFKEQLLAIEPFEEAEILGAIKAVQKETKIKGKNLF
                     MAIRVAVSGQMHGPEIGKTIEVLGRDKSLAHLNSVLEKL"
     gene            complement(5462..>5896)
                     /locus_tag="PLT73_02295"
     CDS             complement(5462..>5896)
                     /locus_tag="PLT73_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRM19244.1"
                     /translation="ILNKLQKEEDIIVESYDGEFEEIPEVDSKLIRLAKLIDGIVITN
                     DYNLNKVCEFQNVPVLNINALANAVKPVVIPGEYMVVTVIKVGTERNQGVAYLDDGTM
                     IVVEEGQHYMNETIEVVVTSALQTAAGRMIFAKPAHSQKGIK"
BASE COUNT         1169 a         1536 c         1498 g         1693 t
ORIGIN      
        1 acaatgaaac gaagcggcct cttctttgta ttgttcacta tcagattgcc ttcactccag
       61 catatcctta tagatctcgc gcaggacctc cagttggtca tccgaccagg tgccgcgttg
      121 gcgttgatca cggaagcggc ggctttcggt ctccaaggct tttttggagc gtttgatgtc
      181 cttgtagagt tccatggcgg agatgcgctg ggcgcctttc gaaaagacga gctgctgttc
      241 ggccaagtcg ctggtggcga catagacttc ggtcagcacg ttgcgcagtt ttttgacgac
      301 gccttcgatg taggtgtcgg cggtctcccc ttgtgcggta aagatgacct tcacccggta
      361 tttagaatac tctttcgtga tgccgggaac gaactgggca tcaaaaacgc accagacttc
      421 gttgccttca taattttgat aatcggacag cgcctgcagc agttgatccc tggcctcttc
      481 gatgaggtcc tggttcttca gcttcaccag ttccggccag gcgccgatca tattgtagcc
      541 gtccactatc aggatttctt tcttcaacag gatcttcacc tcgctttttc tggtacgcag
      601 atgctacgaa tcggatcaaa cgtggcgttt gcggaaaact tcgtacatca tcagtccggc
      661 tgcaacgccg gcattcaggc tctgcacatg gccgaccatc gggatggtca acaggccatc
      721 agcggattcc ttcaacaggc gggaaatgcc tttaccttcg ttgccgatga tcatcgccaa
      781 cggcaacgtt gtgtcccact ggcggtaatc ctgcccggac atgtcggtgg cgtagatcca
      841 gacaccgcgt tccttcaatt cttcgatggt gcggtgcagg ttcgtgacgc gcacgaccgg
      901 tacgtgctcg atggcgcctg tcgatgcttt ggcgacggtc gccgtcaaac cgactgcgcg
      961 gcgcttggga atgatgactc catgggcacc gcaagcatct gcggtacgca gcacggaccc
     1021 caggttatgc ggatcctcga caccatccag gatgatgaag aaaggatctt cgttcttggc
     1081 ggcagccgct tcgaacagat catcgagtac ggcatactgg aaagctgccg tagctgcgat
     1141 gatgccctgg tggacggcgt tatcggacaa cgtgtcgagc ttgcttttcg gtaccgcttg
     1201 gatttggatc ttgcggtcct tcgccagctt gatgatgtca cccaattttt ctccgcccag
     1261 tccatcctgg atgaacagtt tgttgacatc gcgatcggag cgcagcaatt ccaataccgg
     1321 gtggcgtccc atcacgaagt cttcgttttc gggtgtttct ttttctgcgt catcgcgtcg
     1381 tcttggtttt ggttccggtc tgcccttttt cggtgcattg cggctgtcct tgaacgcgcg
     1441 gttcggttta tttttcataa ttgttttcct cgacttcttg gatacaccaa cggatcaact
     1501 cttcaagccg aactttttgc ccggaaagat gcagatagcc catcagcgac tcgaagccgg
     1561 tcgcgatgcg gtaggtcgtt acgtccgcat ttttggcgat cgtatggctc ttgctgttgc
     1621 ggccgcgacg gtacatatcc atttctgctt cgctcaggaa gccttctttt tccagcatcg
     1681 catgcatcag tttcgcttgg gccttggccg aaacgaaaaa agtcgcgcgc cggtgaagct
     1741 ggttgggttt ggtgtgtcct ttttctagta aatgcgttct gatataagtc tcataagcgg
     1801 catcgccgac ataggccaag gcaagaccgt tcaataagct ccaattctgt tctgtcacta
     1861 tgctcttctc catctggtgc cttgggcggt gtcatccaga atgatgccct gatccttcag
     1921 gtaatcgcgg atttcatcac tgcgggcgaa atttttgtct gttcgggctt gtgtccgttc
     1981 atcaatcaaa gtctggatgt cgtcatccag cagtgcttct tcaccgagta cgacgccgaa
     2041 gacgccgacc attgccttgt acaaagccaa tgctgcagtg atgactgctt cagaaacggc
     2101 ttcgccttcc gcgtagatgt tcaattcctt ggcgaactgg taaacgatag acatggcgtt
     2161 atccgcttgg aaatcgtcat ccatttcggc aacgaactgt gcctgcagat tctggaacaa
     2221 agccaattgc tcggcatcat cgggcagtgc cgcaacggca tcttggaggc ggtagtgggc
     2281 attctgatag gcgattttta ctttctccag attgattttg gcttcttcga tggctttttc
     2341 gctgtatttt accggacggc ggtaatgcgc agttccgagg aagaaacgca gcacctgcgg
     2401 atcgacttcc ttcaggatat catgcaccgt cacgaagttc ccgagcgact tgctcatctt
     2461 ctcggctgtt tcaccgattg tgacgaagcc gttgtgcatc cagtagttcg cgaaagtttt
     2521 gccggttttg gcttcgctct gagcgatttc gttttcgtga tgcgggaaag tcaggtcatg
     2581 gccgccaccg tggatatcga tggtgtcgcc caagtatttg gtcgccatca ccgagcattc
     2641 gatgtgccag cccgggcgtc ccggtcccca aggtgattcc caggagactt catccggctt
     2701 cgctgctttc caaagtgcga aatcaagctg atcttccttt ttcacgttct cttccgcatc
     2761 caggcgttcg ctggcgccga cgatcaggtc cttgatcgat tgatcgctca atttgccgta
     2821 gtccttgaac ttaccggtac ggtaatagac gtcgccgtca gcttcatagg catagccttt
     2881 atcgatcaga tcgccgatga aggcgatgat gtccgggata ttttccatca cgcgcggatt
     2941 cagcgtcgct ttttcgacgt gcagagccgc ggtatcttca tagaaggccg cgatgaactt
     3001 ttcagcgact tccttcgcgg aaatgcccaa ctctttggcg gcacggatga tcttgtcgtc
     3061 cacgtcggtg aagttggaga cgaaattcac gtcatagccg cggtattcca agtagcggcg
     3121 gacagtatcg aaagccactg tgctgcgggc attgccgata tggatgtagt tgtagacggt
     3181 cggaccacag acgtacatgc ccaccttatt cttttggatc ggttggaact cttctttttc
     3241 gcgcgtcagg gtattgtaga tttttaacat tgcgcactcc tttccagctt cgaaaaaagg
     3301 gctgacgccc tttttcgttt gctaatcatt cggtcatttt ttcctcactc cgtttatggt
     3361 aacggacgat ttttgccggg atgccgacag ctgtgacatc aggcggaatt tcgctgacga
     3421 cgacagccga tgccccgatt ttggcattgt cgccgatttt gatcggcccc aatacttgca
     3481 catgggcaga caacagcaca ttattcccga tcgtgggatg gcgtttgccg gtatctttcc
     3541 cggtgccgcc cagcgtcacg ccatggtaaa tcatgacatc atcgccgact tcggcggtct
     3601 ccccgatgac gatccccata ccatggtcaa tgaacagacg atgcccgatc gttgcccccg
     3661 gatggatctc gataccggtc aagaaacgcc ccagatgcga aagcacccga ctgaaggtat
     3721 accattttcg cttataaaaa aaatgagcga tatcatatat aaggatggca tgtaaccccg
     3781 ggtacgtcag aacgacttcc aacgtactcc ttgcagccgg atcatgatct tttatggttt
     3841 gaattgtttc gcgtaatcta ttcaagtcat ttcatgctcc tttttggatt atccatccat
     3901 tatatagaat cagctcattt ttactatcct ttttttgtcc gttctatagc ttttccaata
     3961 cgctgttcag gtgagccaag ctcttgtctc tgcccagcac ttcgatggtc ttgccgattt
     4021 caggtccgtg catttggccg ctgacggcga cacggatagc catgaacagg ttcttgcctt
     4081 tgatcttggt ttctttttgg actgctttga tggcacccag aatttcagct tcttcgaaag
     4141 gctcgatggc aagcaattgt tctttgaatg cggcaagcac gaccggcgct gtttcggcag
     4201 ccaggatttc ctgcgcttct gcgtccaggc tgagttcatc agcgaagaac atttccgata
     4261 ggttaacgat ttccgcggca tagctcattt gctcatggta caaaccgatc agctttttcg
     4321 cgtactcact gtcggcttca ctcggatttt ccggcaattt tccggcagcc accaagtgcg
     4381 gcgcggctaa cgttacgatt gtgtccaaat cggcactctt catgtattgg ttgttgatcc
     4441 attccagttt cttctgatcg aaagcggcag gcgatttgct caaacgtgcc ggatcgaaca
     4501 tcttgatcag ctcgtctttc gagaagatct cgtcttcccc gaccggtgac cagcccagca
     4561 gcgtaatgaa gttgaacatc gcttccggca ggtagcccaa gtctttgtat tgttcgatga
     4621 actgcaggat cgtttcatcg cgtttgctca attttttgcc ggtttcgcta ttgatgatca
     4681 acgtcatgtg tccgaaagta ggtattttcc actcgaacgc ttcataaatc atcatttgtt
     4741 tcggtgtatt ggcgatgtgg tcatccccgc gcagaacatg ggtgatttcc atcatgtgat
     4801 catccacggc aaccgcgaag ttataggtcg ggaaaccgtc gcgtttgcgg atgatgtagt
     4861 ccccaccgac gctgctggct tcgaaggtga tcggtccttt gacgatatca tcgaacgtat
     4921 aggtgttttc gatcggtacc cggaaacgga tgactggagt aatgccttgc gcttcttttt
     4981 cagcttgttg ggaaggcgtc aggtttgcgc atttaccgcc gtaatgcggc atttcgccgc
     5041 gtgctctttg gtgttcgcgt tccgtttcca attcatcctc ggtgcaatag cacttgtagg
     5101 cacggttgga aagcaacaat tgatcgatca atggatcgta gatatgctgt ctttcggatt
     5161 gacggtacgg tccgaatttg cctggtttgt ccggaccttc gtcccagtcc atccctaacc
     5221 atttcaagtt ttgcagttgg ctttcttcgc cgtgttcaag gttacgtttt tggtccgtgt
     5281 cttcgatacg gataatgaac tcgccgtcgt tgtggcgagc aaacaggtag ttgaacaatg
     5341 cggtacgagc gtttccgata tgcaagttac cggttgggct tggtgcataa cgtacgcgga
     5401 ttttctttgt catatgaagt atcccctttt ctgtgaagct gtttatttcg tacttgttta
     5461 tttatttgat gcctttttgt gaatgcgcag gcttcgcaaa gatcatgcgg ccggcagctg
     5521 tctgcagggc gcttgtgacg acgacttcga tcgtttcgtt catgtagtgc tgtccctctt
     5581 cgacgacgat catcgtgccg tcatccaggt aggccacccc ttgattgcgt tccgttccga
     5641 ctttgatgac ggtgacgacc atgtattcgc caggtatgac gaccggcttc acagcatttg
     5701 ccaaagcatt gatgttcagc accggcacat tctggaattc gcagactttg ttcaggttat
     5761 agtcgttcgt gatgacgatg ccgtcgatca gtttcgccaa acggatcagc ttgctgtcga
     5821 cttcaggaat ctcttcgaat tcaccgtcat agctctccac gatgatgtct tcttcttttt
     5881 gaagtttgtt caggat
//
LOCUS       DAOMUJ010000052         5892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3668_length_5892_cov_1.977043, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000052 DAOMUJ010000000
VERSION     DAOMUJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_5892_cov_1.977043"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PLT73_02300"
     CDS             complement(<1..152)
                     /locus_tag="PLT73_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19245.1"
                     /translation="MNLDSIWKRRQQLHLKKFSRYSKYIFNDHFVLVLLFLLGALAYQ
                     YSEFVKT"
     gene            complement(149..886)
                     /locus_tag="PLT73_02305"
     CDS             complement(149..886)
                     /locus_tag="PLT73_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19246.1"
                     /translation="MSLLLENVTGGYTQLPVIKNINFEVKAGELTGLIGLNGAGKSTT
                     IKHIIGLMMPFSGKIAIDGRTLREDTERYRQSFAFIPETPVLYEELTLREHLEITAMA
                     YGITEAVAMEKAMRYIRDFRLEDKLDWFPAYFSKGMKQKVMIVCAFMLDVPVYVIDEP
                     FLGLDPLGIHSFLEMVREKKAQGAAILMSTHVLASAEKECDNFVLLHHGEVKVTGTMV
                     DLQHEMGMPGASLDELYISLTKEVAQP"
     gene            1061..1495
                     /locus_tag="PLT73_02310"
     CDS             1061..1495
                     /locus_tag="PLT73_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM19247.1"
                     /translation="MTDCIFCKIANHEIPSSIVYEDDVVIAFLDLSQVTKGHTLLVPK
                     RHVADIFEYDEELAKEVFSRVPKIARAIERSSDDILGLNILNNNRSIAYQSVFHSHIH
                     FLPRYEDSDSDGFGLKWKTHEGKYSQEELAKVVASIKESLEE"
     gene            1498..1878
                     /locus_tag="PLT73_02315"
     CDS             1498..1878
                     /locus_tag="PLT73_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRM19248.1"
                     /translation="MRSFMEGIVFGTVVGGLLGLLNTPRSGKENRAKAKAYLEDNTLA
                     VEDLNESVQGLRKAIQSLTNEGLASVNAVSEEVTKSIESFTQQAQPRINRINDKLAIL
                     TDDMEKVAASMESQMPATTDAPSQ"
     gene            complement(1982..2410)
                     /locus_tag="PLT73_02320"
     CDS             complement(1982..2410)
                     /locus_tag="PLT73_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM19249.1"
                     /translation="MKCEMSSAFCQWLEINNFHLKVESALEKSLQMNHNLSLKEFYVL
                     YFLSQEPEQKLKLQDLEGKVGLSQSAVSRLVSKFEAKGCGALERNVCEHDRRSIYTSL
                     TETGAAKLKAAAATVEQVLTESFSEMPIQILFKQDDIQNK"
     gene            2654..3640
                     /locus_tag="PLT73_02325"
     CDS             2654..3640
                     /locus_tag="PLT73_02325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM19250.1"
                     /translation="MKKKLALTSVAFLTAITLAGCATGETVATTPAGDVTKDELYEAM
                     KASIGETVLQRLILIDVLNDAIGKNELEAETEAELLTTIQQYGGEETFNYILSQSGFA
                     NKEEYQDVLYLNKLIEAAVRAETTFTDEEVEAYYEAYQPQINVQHILVADEATAVDLI
                     NQINEGADFAELAKANSTDTGTASNGGETGLFGKGEMVAAFEEAAYALEVGEVTQTPV
                     ATEYGYHIIKMIEKTEKGTLEEERENIEKVMMDEKLADNDYLTSVMSTIVQAANVEIK
                     DEDLAAAIDQFLPVEETSSSDATSEATTSEETSSEATSATEEAESATSESSN"
     gene            complement(3702..>4508)
                     /locus_tag="PLT73_02330"
     CDS             complement(3702..>4508)
                     /locus_tag="PLT73_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM19251.1"
                     /translation="EEDIQQFQAGQVVAVTGKRELYQNNPQLRITKLRLAREGEPSRP
                     ELYMERAPLKSEEMVEEVSQILFEITSAPINRIVRHILKKYQREFFEYPAAKRHHHAI
                     VGGLGFHTVSILRMAKTVIQHYPSVNKSLLYGGVILHDIGKTIELSGSMSTEYTLKGN
                     LIGHIVLIDEEITKACLSLNIDEDSEEVILLKHIVLAHHGKQEFGSPVTPHLLEAEII
                     HHLDNLDASINMIDTALLRTTPGTFSERIFGLENRSFYKPLFVQPSEEGQ"
     assembly_gap    4509..4518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4519..4669)
                     /locus_tag="PLT73_02335"
                     /pseudo
     CDS             complement(4519..4669)
                     /locus_tag="PLT73_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075929.1"
                     /note="catalyzes the exonucleic cleavage of mRNA yielding
                     nucleioside 5'-phosphates; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(4691..>5892)
                     /locus_tag="PLT73_02340"
     CDS             complement(4691..>5892)
                     /locus_tag="PLT73_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19252.1"
                     /translation="LNDREELEERGKILKTEWEIFLQQKGYPARMTAPELLQLDPGAV
                     LADKSTLIIKQEAEMDDLSLAVDKWNEQTAFIRSRFHFEHLTVTEMAARFPEILNAVV
                     LEESMAANVQDKINELQQELELIQRDLAESHRQRQQLLDQANAKTEAEFYQLLALKEK
                     QEQNLKRKEFLDEQVNGREQLLDRYGDAKKSMSLYEKNDLEMQQLKAELSRLQRTEVE
                     LKHQLEQLEEGGTYSALLQEFALAETELRDLAVEWAGLVTAGEWIERALRYGKEDRLP
                     LMLDDTITFFSRLTEGAYTGIAFQKNGMKVQRKDGTTFFPNELSQGTVEQLYIALRLA
                     FVKNTADIVSMPILIDDGFVNFDEDRKNIMFTLLEELSEDVQVLFFTFDDACVQRFSQ
                     KQVYMLK"
BASE COUNT         1581 a         1444 c         1290 g         1567 t
ORIGIN      
        1 gttttcacga attcggaata ttgataagcc aaagcgccca acagaaagag gagcacgagg
       61 acgaaatgat cattgaagat atatttgctg tagcggctga attttttcag gtgcagttgc
      121 tgccttcttt tccagatact gtccaagttc atggttgcgc cacttctttc gtcaagctga
      181 tgtaaagttc atcgagactt gctcccggca tacccatttc atgctgcagg tctaccatcg
      241 tgcctgtcac tttgacctca ccatggtgca gcaggacgaa attatcacat tctttctcgg
      301 cagacgccaa tacatgcgtg gacatcagaa tagctgcacc ttgcgctttt ttctccctga
      361 ccatctccag gaacgagtgg atgccaagcg gatccagacc caggaatggt tcatcgatga
      421 cgtataccgg cacatccaac atgaacgcgc aaacgatcat cactttttgc ttcatgcctt
      481 ttgaaaaata agccggaaac cagtccagct tgtcctccag acgaaaatct cggatgtagc
      541 gcatcgcctt ttccatggcg actgcttccg tgattccgta ggccatcgcc gttatttcca
      601 gatgttccct taaggtgagc tcttcataga gcaccggcgt ctccggaata aaagcgaagg
      661 actgacgata tcgttctgta tcctcgcgca atgtccggcc atcgatcgca atctttccgc
      721 tgaaaggcat catcagaccg atgatgtgtt tgatggtcgt actcttgcct gctccgttca
      781 atccgatcaa tccagtcagt tcaccagctt tgacctcaaa atttatattt tttatgacgg
      841 gcaattgtgt atagccgccc gtgacattct ccaataatag tgacatttaa ccgtcccttt
      901 cacatgcgtt tgtgccttat attataacac agcctcgccc caacccaaat atcttgctga
      961 gcgaagcgcc attttgccct caggatatca aaaaaaacgc aaatatgata aagttatgct
     1021 atagaataaa gtagataatg gacagaaaga ggtcgagcct atgacagatt gtattttttg
     1081 caaaatcgcg aaccacgaaa tcccgagcag cattgtctat gaagatgatg tcgtcattgc
     1141 gtttctggat ttatcccaag tgacgaaagg tcatacactg ctggtaccca agaggcatgt
     1201 cgctgatatt tttgaatatg atgaagaatt ggcgaaggaa gtcttttcgc gcgtccctaa
     1261 aatcgcacgc gcaatcgaac gttccagcga tgacatttta gggctgaata ttctgaacaa
     1321 taaccgttcg atagcttatc aatccgtttt tcattcgcat atccatttct taccgcgtta
     1381 tgaagattcg gactcggatg gtttcggttt gaaatggaaa acgcacgaag gaaaatactc
     1441 acaggaagaa ttggcgaaag tagtagcatc catcaaagag agtctggagg aatagatatg
     1501 cgctcattta tggaaggaat agtattcggt acggtagtcg gcggtctgct ggggttgctg
     1561 aacaccccgc gcagcggaaa agaaaaccgc gcgaaagcga aagcttacct tgaggacaac
     1621 acacttgcgg tagaggatct caatgagagc gttcaaggtt tgcgcaaagc gatccaatcc
     1681 ttgaccaacg aaggattggc ttccgtcaat gccgtttcag aggaagtcac caaatcgatt
     1741 gagagcttta cgcaacaggc ccaaccaaga atcaaccgca tcaacgataa acttgccatt
     1801 ttgactgatg atatggaaaa agtcgcagca agcatggaat cgcaaatgcc tgcaacgaca
     1861 gatgccccat cacaataatg tacattaaaa atacgacttg ccaaatgcac accggatcat
     1921 cggtcatttg gcaagtcgta ttttttttac ttcctatcaa aaagactcca atcaagaagc
     1981 tctacttatt ttgaatatca tcttgtttaa acagtatttg gattggcatt tccgaaaaag
     2041 actccgtgag tacctgttcc accgtcgcag ccgctgcctt cagttttgcc gcacccgttt
     2101 cggttaagga cgtatagatg ctccgccgat catgctcgca gacatttcgc tccaacgcac
     2161 cacagccctt agcctcaaac ttgctgacca gtctcgatac cgcactttgg cttaagccaa
     2221 cctttccctc caaatcctgg agtttcagtt tttgctccgg ttcctgtgat aagaaataaa
     2281 ggacatagaa ctctttcaga gaaagattat ggttcatctg caaacttttt tccaatgcgc
     2341 tctcgacttt cagatgaaaa ttattgatct ccagccattg gcaaaacgca gaactcattt
     2401 cacatttcac aaagtcccct ccatcccttt cgaattatca acttatgata ccaaatattg
     2461 gcattttgag caatctgccg cgactcgacg cggatacttt gtatactttc ggttttatct
     2521 gcagatatga attttctgag aagtttgcta gcgtttttaa ggaatatttt ccataatcac
     2581 ggcgtttatg atatatttat agatggttat tcatgggata gataaataga aatagattgg
     2641 aagtgccaca ttcatgaaga agaaattagc attgacgtcc gttgctttcc tgacggccat
     2701 cacgttagca ggctgtgcga ctggcgaaac tgtcgcaacg actccagcag gcgatgtcac
     2761 gaaagatgaa ctatatgaag caatgaaagc ctcgatcggt gaaaccgtct tgcaacgtct
     2821 gatcttgatc gacgtattga acgatgcgat cggcaagaat gagttggaag cggagacgga
     2881 agctgaactg ttgacgacca tccaacaata cggcggtgaa gaaacattca actatatctt
     2941 gtcacaatca ggcttcgcaa ataaggaaga ataccaagat gttctatatt tgaacaaact
     3001 gatcgaagca gcagtaagag ctgaaacaac tttcacggat gaagaagtgg aggcttatta
     3061 tgaagcttac caaccacaaa tcaacgtaca gcatatcttg gttgcggatg aagcgactgc
     3121 tgtagacttg atcaaccaga tcaatgaagg cgcggatttc gctgaattgg caaaagccaa
     3181 ctcgactgat accggaacag cttcaaatgg cggagaaaca ggattgttcg gaaaaggcga
     3241 aatggtcgca gcattcgaag aagctgctta tgcgcttgaa gtcggtgaag tgacacaaac
     3301 accagtcgct accgaatacg gctaccacat catcaaaatg atcgagaaga cagaaaaagg
     3361 aacattggaa gaagaacgcg aaaacatcga gaaagtgatg atggacgaaa aacttgccga
     3421 caatgattat ctgacatccg tcatgtccac aatcgtccaa gccgcgaacg ttgaaatcaa
     3481 agacgaagac ctggcagcag cgatcgatca attcttacct gttgaagaaa ctagttcatc
     3541 agatgcaaca tccgaagcaa caacttcaga agagactagc tcagaagcaa cttctgcaac
     3601 tgaagaagcg gaatcagcta catccgaatc ttcaaactaa taccaataac gtaaaacacg
     3661 caagctaccg gctcttgatt ccggtaactt gcgtgttttt gttattgccc ttcttcagaa
     3721 ggttggacaa agagcggctt atagaagctg cggttctcca acccgaaaat gcgctcgctg
     3781 aacgttcctg gcgtcgtgcg caacaaagcc gtatcgatca tattgatgga agcatccaaa
     3841 ttgtccagat gatggatgat ttcggcttcc aggagatgcg gtgttaccgg cgagccgaat
     3901 tcctgtttgc cgtgatgcgc cagaacaatg tgtttcaaca gaatgacttc ttcactatcc
     3961 tcatcgatgt tcaaagagag acaggctttg gtgatttctt catcgataag gacgatgtgt
     4021 ccgatcaggt tccctttcag ggtatactct gtcgacatac taccactcaa ctcaatcgtt
     4081 tttccgatgt catggagaat aacgccaccg tacaacaaag acttattgac tgatggatag
     4141 tgttgaatga ccgttttcgc catccgcaaa atcgaaaccg tatggaaacc cagtccgccc
     4201 acgatggcat gatgatgacg cttcgcagcg ggatactcga aaaattcccg ttgatatttt
     4261 ttgagtatat gccggacaat ccggttgatc ggcgcgctcg tgatctcaaa caaaatttgg
     4321 ctgacttcct ccaccatttc ttccgatttc aacggagcac gttccatata aagttcaggt
     4381 cggcttggtt ccccttccct agcgagcctt agctttgtga tgcgcagttg gggattgttc
     4441 tgatacagtt cccgtttgcc ggttacggca acgacttgac cagcttggaa ttgctggatg
     4501 tcctcctcnn nnnnnnnnag tatttcccat cgatctgacc gctgcggtcc tggaaagtaa
     4561 aggcaatgaa tttcttgccg tttttcgcca gacggatatc cgcacttttg atcagtacat
     4621 aaatttcaaa attatcatcg atttcatgtt cgtatatttt tttggccacc cctaccagcc
     4681 cctttcactc ctattttaac atgtatactt gtttctgtga aaaacgttga acgcaagcgt
     4741 catcgaatgt gaaaaagagt acctgcacgt cttcgctcag ctcctccagc aaagtaaaca
     4801 tgatgttctt cctgtcctcg tcgaagttca caaaaccatc atcgatcaag atcggcatgc
     4861 tgacgatatc ggcagtgttc ttcacaaacg ccaaccgcag cgcaatatac aactgttcca
     4921 ctgtcccctg cgacaattca ttcgggaaaa aggttgttcc gtccttgcgc tgcaccttca
     4981 taccgttctt ttggaacgcg atgccggtgt atgcaccttc cgtcaaacga ctgaagaagg
     5041 ttatcgtatc gtcgagcatc agcggcaggc gatcctcttt tccataccgc agtgcccgtt
     5101 ctatccattc tccggcagtg accaagccag cccactcgac ggcaagatcc ctcagctcgg
     5161 tttcggccaa agcaaattcc tgcagcaagg cgctgtatgt cccgccttct tccaattgtt
     5221 ccagctgatg cttcagttct acttccgttc tttgcagtct cgacaattcg gccttcagct
     5281 gctgcatttc caaatcattc ttttcgtaaa gggacatgct tttcttcgca tcgccgtatc
     5341 gatccaggag ttgctcgcgg ccattgacct gttcatccag aaattccttg cgcttgaggt
     5401 tttgttcttg cttctccttc aacgccagca actggtagaa ctcggcttcg gtctttgcgt
     5461 tggcctgatc caagagctgc tgccgctgtc gatgagactc agcaaggtcg cgttggatca
     5521 gttccaactc ttgctgcagt tcgttgattt tatcctgaac atttgcagcc atgctttctt
     5581 ccaaaacgac ggcattcagt atctcgggga agcgtgcggc catttcagtg accgtcagat
     5641 gttcaaaatg gaaacggctg cggatgaacg cagtctgctc gttccatttg tccacggcga
     5701 gacttagatc gtccatttcg gcttcctgtt tgatgatcaa ggtggattta tccgccaaca
     5761 cggcaccggg atccaattgc aggagttccg gtgccgtcat gcgggccgga tagcctttct
     5821 gctgcaggaa gatctcccat tcggttttga gtatctttcc gcgttcctcc agctcctcgc
     5881 ggtcgttcaa aa
//
LOCUS       DAOMUJ010000053         5832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3722_length_5832_cov_2.499221, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000053 DAOMUJ010000000
VERSION     DAOMUJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_5832_cov_2.499221"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..199)
                     /locus_tag="PLT73_02345"
     CDS             complement(59..199)
                     /locus_tag="PLT73_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19253.1"
                     /translation="MGLSNNREESDNASKHTTSASHLVRYLSDNRIVSDIRIAFGAVL
                     VG"
     gene            complement(324..1277)
                     /locus_tag="PLT73_02350"
     CDS             complement(324..1277)
                     /locus_tag="PLT73_02350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRM19254.1"
                     /translation="MRRVYTIGEILIDFFPSEIGVPLKEVSGFTKQPGGAPANVAVTV
                     SRLGGNASFIGKVGQDAFGDYLTDVLKQNQVDTRHLFRTTEANTALAFVSLQENGERE
                     FSFYRNPSADMLLNKEELNELVFTAADILHFCSVDLIEAPVKYAHLAALEKMQQAGGT
                     IVFDPNVRLNLWPDPEMLKQTIQEFLPYADILKISDDELAFITGMDSEADGIAALLKT
                     EPKLLIFTKGSKGADLYFKGEKASVEGISVKAVDTTGAGDSFIGSFLYQIAGKDAWED
                     CTLDEIREMGLFANRVAALVTTRPGGMQSIPTLQEVLRSEG"
     gene            complement(1329..2828)
                     /locus_tag="PLT73_02355"
     CDS             complement(1329..2828)
                     /locus_tag="PLT73_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM19255.1"
                     /translation="MSIQSQTAYSTDRANQYIAENKGTVNPLYRNRYHASPPVGWMND
                     PNGFVQFQGAYHLFYQFHPYDAKWGPMHWGHMKSTDLIHWEDLPVALAPDQAYDRSGC
                     FSGSAIERDGKLYLLYTGVTEANEEGVHHQVQCLAVSEDGVNFEKVQQNPVIPVAALP
                     ADASQIDFRDPKVFEHEGMYYTVVASKAGDETGQILLYQSADLVEWEFASVFLKGNKE
                     QGIMWECPDFFRLNGKDCLIFSPMQWPQDGDDYQNLNSSVLAVGEVDWATKQFHPESY
                     QELDHGLDYYAPQSLEDDKGRRICIGWMQMWGRNFPTDTLGHNWTGSMTIPRELKWED
                     GKLKQYPIGIEAIADNQGENSPVITMVDTKESIPGIAGDCGLLTLKVDTSGLEKLSIH
                     LRENENERTVVYYDKAKNELGFDRSASGIELKGEEAHPPVSRKVTLSGKSDHLELAIY
                     LDSSSVEVFEKNGEVVMTSNVYPTAEAKGISFECSGKAVFEEATFTPFI"
     gene            complement(2870..4483)
                     /locus_tag="PLT73_02360"
     CDS             complement(2870..4483)
                     /locus_tag="PLT73_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19256.1"
                     /translation="MRAKYLGTALLSTATVLTLAACGSSGGASSPDYELTDVSFPLEE
                     TVSLKMSTSSSPLAPADPNEKLIFQRLEEQSGVNIEWKNYSSDYIEKRNLDISSGDLP
                     DAMWNAGASDYDLLSWAEDGIIIPLEDLINEHMPNFKKVLDENPEYLAMITAPDGHIY
                     SLPWIEELGQDKESIHTVNDIPWINVDWLEALGLEMPQTTDELMVVLEAFKTQDPNGN
                     GEADEIPISFINDGGNEDMKFLFGAFGIGDNDDHLVVNDDGTIDFTADNEEFKNGVAY
                     FNEMYNKELIDVEAFEQDWNAYVAKGKEQLFGVYFTWDKANVSGANDSYEPLPALAGP
                     SGEKHVTRTNGFGFSRDRFVITSANKNLELTAKWADQMYVPIQSVQNNWGTYGDETQQ
                     NIFEYVEATNSLKHLPLEGTAPGELRQKTEAGGPLAILDDYYGTVTTMPDDAQWRLDI
                     FKDTYLADVHNQFNYPRVFLSLEQADRIAQIEADLMPFVSRKRAEWIANGKVAEEWDA
                     YLEELNRLGLEEWMQIKQEAYDTYLSNNK"
     gene            complement(4530..5411)
                     /locus_tag="PLT73_02365"
     CDS             complement(4530..5411)
                     /locus_tag="PLT73_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM19257.1"
                     /translation="MITLTKFDRGVLLANRILIGFLVLITLSPLLYVLIASFMDPFIL
                     RSQGLSLNPANWSLDGYKRVLQDPAIIRGFVNSIFYSFTYSALTVAVSILTAYPLSKQ
                     KLVGKRPITIFLMITMFIGGGLVPTYLLVKNLGMLNTVWAIILPGSINVWNIILARTY
                     FKQLPAELEEAAIIDGANEMQIFFKIMLPLAKPIIFVLFLYAFVGQWNSYFDAMIYLN
                     DADLQPLQLVLRKILIQNQPQQDMIGSATQMAEMAQLAELIKYSTIVISSLPLLVMYP
                     FFQKYFDKGMMAGSLKG"
     gene            complement(5426..>5832)
                     /locus_tag="PLT73_02370"
     CDS             complement(5426..>5832)
                     /locus_tag="PLT73_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM19258.1"
                     /translation="GIWQGMGWASVLYTATLANVSQDLIDAAHMDGANILYRIWYIDL
                     PAIRPIMVIQFILSVGGIMNVGYEKAFLMQTSMNLPVSEIISTYVYKVGLQMGDYGYS
                     TAVGLFNTVINIILLLAVNALANKVSNDDAAL"
BASE COUNT         1390 a         1519 c         1327 g         1596 t
ORIGIN      
        1 ttgcagtaat ccgacaagca gtgcaccaaa tgcggtgagg ttgtcggata cattgcggtt
       61 atccgacaag cactgcacca aatgcgatgc ggatgtcgga tactatgcgg ttatccgaca
      121 agtagcgcac caaatgcgat gcggatgtcg tatgcttgct cgcgttgtcc gattcttccc
      181 ggttattgga caaccccaac aacccagcca tctccgatcg aacccagata tgaaaaaaca
      241 cattcaagta cccgtaagca ccaagtgaac atgcaaaaaa cagggctatc cacataggaa
      301 taatgccctg tttttttgat taattatcct tccgaacgta atacttcctg caaggtcggt
      361 atggattgca tgccgcccgg tcgcgtcgtc accaatgcgg ccacacgatt ggcgaacagg
      421 cccatctcgc gtatttcatc caacgtgcag tcttcccaag catccttgcc ggcgatctga
      481 tagaggaagg acccgatgaa tgagtcgcct gcccctgtcg tatcgactgc tttaacgctg
      541 attccttcaa cactggcttt ctcgcctttg aagtacagat ccgctccctt gctgcctttg
      601 gtgaagatca gcagtttcgg ttccgtcttc agcaatgctg ctatcccatc cgcttccgaa
      661 tccatgcccg taatgaaggc caattcatca tcgctgattt tgaggatgtc ggcatatggc
      721 aggaattctt ggatcgtctg cttcagcatt tccggatccg gccagagatt caagcgcaca
      781 ttcgggtcaa aaacaatcgt tccgccggcc tgctgcattt tttccaaagc ggcaagatga
      841 gcatacttta cgggtgcttc gatcagatcg accgagcaaa aatgcaggat gtctgcagcg
      901 gtgaaaacaa gctcattcag ttcttccttg ttcaacaaca tatccgcgct gggattgcgg
      961 tagaaactga attcgcgctc cccattttcc tggagcgaca caaacgccag cgccgtgttt
     1021 gcctcagtgg tccggaacag gtgacgggta tccacctgat tctgtttgag cacatcagtc
     1081 agataatccc cgaaggcatc ctgacccact ttaccgatga agctcgcgtt cccgcctagg
     1141 cggctaaccg tcaccgcaac attagcaggc gcgcctcccg gttgcttcgt aaaaccggaa
     1201 acctccttca agggcacccc gatttcactc gggaaaaaat caatcagtat ttctccaata
     1261 gtataaactc tccgcataac gtctcctttc ggttgcagtt atttgttcca aaaaacctga
     1321 attcccgatc agataaaggg cgtaaaagtc gcttcctcaa ataccgcttt gccgctgcat
     1381 tcaaaggaga tgcctttcgc ttccgcagtt ggatatacgt ttgaagtcat gactacttca
     1441 ccatttttct caaacacttc aaccgacgaa ctgtccaggt aaatggcgag ttcaagatga
     1501 tcgctctttc cggacaacgt gactttccgg ctgacgggag gatgtgcttc ctcccctttc
     1561 agttcgatgc cgcttgcaga tcgatcgaat cctaattcat tctttgcttt atcatagtag
     1621 actacggttc tttcgttctc gttctcacgc agatggatgg acagtttttc caatccggac
     1681 gtatccactt tcagcgtcag caatccgcag tcccctgcga tgcctgggat tgattcttta
     1741 gtatccacca ttgtgatcac cgggctgttt tctccctgat tgtcggcaat ggcttcgata
     1801 ccgatcggat attgcttcaa cttgccgtct tcccatttca actcccgcgg aatcgtcatt
     1861 gatcctgtcc agttgtggcc cagcgtatct gtcgggaaat ttcttcccca catctgcatc
     1921 cagccgatac agattcttct gcctttgtca tcctccaggc tttgaggagc ataatagtcc
     1981 aaaccgtgat ccaattcctg ataactttcc gggtggaact gtttcgtcgc ccaatccact
     2041 tcgccgacag ccaatacaga cgaattcaga ttctgataat catccccatc ttgcggccat
     2101 tgcatcggcg aaaagatcaa gcaatctttc ccattcaagc ggaagaaatc cgggcattcc
     2161 cacatgatcc cttgctcttt gttccccttc aggaagacgg aggcaaactc ccattccacc
     2221 aagtcagcag attggtaaag caaaatttgc cctgtctcgt cgccagcctt ggaggcgact
     2281 accgtgtagt acatgccttc gtgttcaaaa actttagggt cccggaaatc gatctgggaa
     2341 gcgtctgccg gcaaagccgc aaccgggatg accggatttt gctgcacttt ctcgaagttc
     2401 accccgtcct ccgataccgc caagcactgc acctggtgat gcacgccttc ttcattggct
     2461 tcggttacgc ctgtataaag cagatacaat ttcccgtccc gctcgatggc gcttcctgag
     2521 aaacacccgg accggtcata cgcttgatcc ggtgccaagg cgacaggcaa gtcttcccaa
     2581 tggatcaagt ccgtcgactt catgtggccc cagtgcatcg ggccccattt cgcatcatac
     2641 gggtggaatt gataaaacag gtgatacgcc ccttggaact ggacgaagcc gttcggatcg
     2701 ttcatccagc cgactggcgg tgatgcatga tagcgattcc ggtaaagagg attcactgtt
     2761 cctttatttt cagctatata ttggttggca cgatctgttg aataggctgt ttgactttgt
     2821 atactcacgc tacccctcat ttcttggatt tgttttattt tctatgtggt tatttattgt
     2881 ttgataaata tgtatcataa gcttcttgtt tgatctgcat ccattcttcc agtcccagac
     2941 ggttcagttc ttccaggtaa gcatcccact cttcggcaac tttgccgttt gcgatccatt
     3001 ccgcacgttt tctgcttacg aacggcatca aatcagcttc gatctgtgcg atccggtctg
     3061 cctgttccaa tgacaagaat acgcgaggat aattgaattg attatgaaca tcagccaagt
     3121 aagtgtcttt gaagatgtcc aaacgccatt gtgcatcatc cggcattgtc gtcacagttc
     3181 cgtagtagtc gtccaaaatt gccaacggtc cgccagcttc ggttttttga cgcaattcgc
     3241 ccggtgctgt gccttccaaa ggcaagtgtt tcaacgaatt ggttgcttct acatattcaa
     3301 agatattttg ttgcgtttca tccccatatg taccccagtt attctgtaca gattggatcg
     3361 gcacatacat ctgatctgcc catttggcag tcaactccag atttttgttg gcgctggtga
     3421 tgacgaaacg gtcacggctg aatccgaagc cgttcgtacg cgtaacatgt ttttctccac
     3481 ttggaccagc caatgcaggc agtggctcat aggaatcgtt cgctccggaa acattcgctt
     3541 tatcccatgt gaagtacaca ccgaacaatt gctctttccc tttcgccacg taagcattcc
     3601 aatcctgttc gaaagcttcc acatcgatca gctctttgtt gtacatttca ttgaaataag
     3661 cgacaccgtt tttgaattct tcgttgtcgg ccgtgaagtc gatcgtgccg tcatcattta
     3721 cgaccaagtg atcatcattg tcgccgattc cgaatgcgcc gaagaggaac ttcatgtctt
     3781 cattcccgcc atcgttgatg aaagaaatcg ggatttcatc ggcttcgccg ttgccgttcg
     3841 ggtcttgagt tttgaatgct tccaatacaa ccatcaattc atccgtcgtt tgcggcatct
     3901 ccaagcctaa agcttccagc cagtcgacat tgatccaagg gatatcgttt accgtatgga
     3961 tggactcttt atcctggccc aactcttcga tccatggcag tgagtagata tggccgtccg
     4021 gtgcagtgat catggccaaa tattccggat tttcgtccaa tacttttttg aagttcggca
     4081 tgtgttcatt gatcagatct tccaaaggaa tgatgatgcc gtcttctgcc catgagagca
     4141 gatcatagtc actcgcgcca gcgttccaca tggcatccgg caagtcgccg ctggaaatgt
     4201 ccagattccg tttttcgata tagtcagaag aataattttt ccattcgata ttgacgccgg
     4261 attgttcttc caaacgttgg aaaatcagct tttcgttcgg atcggccgga gccaacggag
     4321 aactggaagt ggacattttc aagctgacgg tctcttccaa tgggaaggat acatccgtca
     4381 attcgtagtc tggagaagag gcgccaccgg atgaaccgca ggctgctaaa gtcaggactg
     4441 ttgctgtgga taatagtgct gtgccaaggt attttgctct cattttgtgt ttctccttta
     4501 taatgtattt attttgtgtt gcgtattgct taacctttga gtgaacctgc catcatccct
     4561 ttatcgaagt acttctggaa aaatggatac atgaccagaa gcggcaagct ggagatgacg
     4621 attgtcgagt atttgatcag ttcggccaat tgcgccattt ctgccatctg agtggcggat
     4681 ccgatcatgt cttgttgcgg ttgattttgg atcagtattt tccgcagcac caattgcagc
     4741 ggctgcagat ctgcatcgtt caagtagatc atggcgtcga agtaggaatt ccactggccc
     4801 acaaaagcat agaggaacaa tacgaagatg atcggtttcg ccagcggcag catgattttg
     4861 aagaatatct gcatttcatt ggcgccgtcg ataatcgcag cttcttccaa ctcggcaggc
     4921 agttgtttga agtacgttcg cgccaggatg atgttccaga cgttgatcga gccgggaagg
     4981 atgatggccc agaccgtatt cagcatgccc aggtttttca ccaataggta agttggaacc
     5041 aagccgcctc cgatgaacat cgtaatcatc aggaaaatcg tgatcggccg ttttccgacc
     5101 aatttctgtt tggacaacgg ataggcggtc agaatggaga ccgcgacagt caacgcgctg
     5161 tatgtgaatg aatagaaaat cgaattcacg aaacctcgga tgatggccgg atcctgtaag
     5221 accctcttat agccgtccag actccagttc gctgggttca gactcaatcc ttgcgagcgg
     5281 aggatgaagg gatccatgaa agaagcgatc aatacgtaca acaaagggga tagggttatc
     5341 aagaccagaa agcctatcag aatgcgattc gccagcagca cgccgcggtc aaattttgtc
     5401 aatgttatca tttttgttct cctctctata aagctgcatc atcattgctt actttgttcg
     5461 ctaacgcatt tacggccagc aacaagatga tattgatgac tgtattgaat agtccgactg
     5521 ccgttgaata cccgtaatca cccatctgca gaccgacttt gtagacgtag gtcgagatga
     5581 tttccgaaac aggcaggttc atcgatgtct gcatcaggaa agctttctcg tagccgacat
     5641 tcatgatgcc ccctacagaa aggatgaact ggatgaccat gattggtcgg atcgcaggca
     5701 gatcgatgta ccagatacga tagaggatgt tagcgccgtc catgtgggct gcatcgatca
     5761 aatcttgact cacgttggcc aatgtcgccg tgtagagcac cgatgcccaa cccatacctt
     5821 gccaaatacc gg
//
LOCUS       DAOMUJ010000054         5802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3751_length_5802_cov_1.859231, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000054 DAOMUJ010000000
VERSION     DAOMUJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_5802_cov_1.859231"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="uvrC"
                     /locus_tag="PLT73_02375"
     CDS             complement(<1..752)
                     /gene="uvrC"
                     /locus_tag="PLT73_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRM19259.1"
                     /translation="MSREEINQKLALLPDLPGCYIMRDKDDEIIYIGKAKNLKNRVKS
                     YFHSKHEGKTQLLVADIDRFETIVTKTNKESLLLEINLIKRYQPKYNIKLKQGTMYPY
                     LKVTNEKDPQLIITSTVEDDGGIYFGPYPHVYAATETQEFIQKIYPLRKCAKNSKRAC
                     LYYHMGQCIGCCDHEVSKEEYDAQIKRIAAFLNGDVKEIKKDLREKMHRAAESLNFEQ
                     AADYRDQISYIEATVEKQTVMSRDYTNRDVFA"
     gene            883..2136
                     /locus_tag="PLT73_02380"
     CDS             883..2136
                     /locus_tag="PLT73_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM19260.1"
                     /translation="MIKQIKYEKWQRQTALFLGSQTISLFGSSLVQYAIFWYLTLETQ
                     SGVIMTLSTIFGFLPTFFISPFAGVWADRYNRKRLIVLSDGIIALSTLVLVLLFLAGQ
                     RSIWILLATSAIRALGAGIQMPAVGAILPQIAPEKELTRINGLNGSIQAVIMLVSPMI
                     SGALYQFAPMEGIFLIDIVTAALAIAILVFFLKVPTHEKASQEQVSNYLQDMKLGFSY
                     IQNHTFIKRLFLYFSLAFFMAAPVSFLSPLQVARSFGDDVWRLTAIEIAFSIGMIGGG
                     LWIASWGGFKNRIHSIAAAIAVMGLCSFGMGVIPNFWIYLFLMGLVGLFIPLLNAPSM
                     TLLQEKVEEDFLGRVFGVQSMVASAMMPLGMLVFGPLADRMAIEILMAVSGLFLMVVA
                     FFAIRDRVLLEAGKPEPSDTGKESN"
     gene            complement(2306..4879)
                     /locus_tag="PLT73_02385"
     CDS             complement(2306..4879)
                     /locus_tag="PLT73_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRM19261.1"
                     /translation="MDATKTTFKAGFEKLNKDIERFPHVFPITEDMHITYEGVSRLVM
                     LDRYSYKDSTKETLSEGDLVILTVKEDPKYPARGTGTILSINLKDQTARIRVSAEYQH
                     NIDDFEVEEGGIVTRRILTLDKPLELFYEQIAMRNAHGLAEVEITPELRHEAFLKFYE
                     EQKALNFIPAGRVLYGAGSGTDVTYFNCYVMPFVPDSRGGISDHRKKVMEIMSRGGGV
                     GSNGSTLRPRHTIVKGVNRRSSGSVSWMDDIAKLTHLVEQGGSRRGAQMIMLADWHPD
                     IFEFIISKMQNPRILRYIIENFEDEQIRMLAKEKLHFKPFSPKEINMYTGIVNYKQIP
                     GHGGFDASVIHEAEKKLRDGGTYSVNNPEFLTGANISVCITDDFMDAVMRGEEYALRF
                     PDVEHYDADAMAHYDAEWTNCGDVREWEATGNAVRTYRTVKARELWRLINVCATYAAE
                     PGIFFIDNANKMTNATAYGQKVVATNPCGEQPLAAYSVCNLAAVNLAEMVNKDLQMVD
                     FAKLEQTVRTGIHMQDNVIDSTPYFLEENKKQALGERRIGLGIMGLADMLIYCGVRYG
                     SLESLQLIDQVFETIAVAAYEESIELAKTRGSFPFLVGQSGKETQILRERFINTGYMK
                     KMPEHIREGVLKYGIRNSHLLTVAPTGSTGTMAGVSTGLEPYFSFTYYRSGRLGKFIE
                     VKADIVQEYLDRHPDADPMNLPDYFVAAMTLAPEEHVDVQTTIQRWVDSSISKTVNAP
                     KGYTVDQVEKIYERLYLGGAKGGTVYVDGSRDSQVLTLKAEENVWDEEGKLEEEKEHV
                     KINKDKTFLVDSIANLEATDVTIGNEIGDTCPICRQGTVEDLGGCNTCTNCGAQLKCG
                     L"
     gene            5303..5758
                     /locus_tag="PLT73_02390"
     CDS             5303..5758
                     /locus_tag="PLT73_02390"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRM19262.1"
                     /translation="MNDKERFADMIRKSKRIVAFTGAGISTESGIPDFRSAGGLFDRL
                     SGRHFTGEETLSIGFLETYPQLFFRNYAEHFNFSQARPNAGHRFFARLEHAGKEVTVV
                     TQNIDGLHQKAGNSPANVIEVHGTVREFVSEFRGIAGTARVAGALQAAP"
BASE COUNT         1240 a         1510 c         1564 g         1488 t
ORIGIN      
        1 aaggcgaaga cgtcgcggtt cgtgtagtcg cgcgacatga ccgtctgttt ttccacagtc
       61 gcctcgatgt agctgatctg atcgcggtaa tcggcagcct gttcgaaatt caggctttct
      121 gcggcgcggt gcattttttc gcgcagatct ttcttgatct ccttgacgtc gccattcagg
      181 aaagcggcga tgcgtttgat ttgggcatcg tattcctctt ttgacacctc atgatcgcag
      241 cagccgatgc attggcccat atggtaatag aggcaggcgc gtttcgaatt cttggcgcac
      301 ttgcgcaacg gatagatttt ttggatgaat tcctgcgtct ccgtcgccgc gtagacgtgc
      361 ggatagggtc caaagtagat gccgccgtca tcctcgaccg tcgaagtgat gatgagctgc
      421 ggatcttttt cgttcgtgac cttcagataa ggatacattg tcccttgttt cagtttgatg
      481 ttgtatttgg gctggtaacg cttgatcagg ttgatctcca gcagcagcga ttccttgttc
      541 gttttggtta cgatcgtttc gaaacggtca atgtccgcga ccaacagctg cgtcttgcct
      601 tcgtgcttgc tgtggaagta cgatttaact cgattcttca gatttttggc cttgccgatg
      661 tagatgattt cgtcatcctt gtcccgcatg atgtagcagc cgggcaagtc gggcagcagc
      721 gctaattttt ggttgatttc ttctctgctc atccgtttca atccttttac ttttcgttga
      781 gaatagtata ccatgaatgg aaacatacgt tcagtcgttt gattctgaac gcagtcattt
      841 tggggacgat aaccgcctct ccagcaagga agtgcaaaca atgtgatcaa gcaaataaaa
      901 tatgaaaagt ggcagcgtca aaccgctctt tttctgggca gccagacgat ttccctgttc
      961 ggctcgtcgc ttgtccaata cgcgatattc tggtacctga ccttggaaac acaatccggc
     1021 gtcatcatga ccttatcgac catcttcggc tttctaccaa ctttcttcat ctcgccattc
     1081 gccggggtgt gggccgaccg ctataaccgg aagcgcctga tcgtcctttc cgacggcatc
     1141 attgcgctct cgactttggt gctggtcctg ctgttcttgg cggggcagcg ttccatctgg
     1201 attcttttgg cgacatcggc catccgcgca ttgggtgcgg gcatccagat gccggcggtc
     1261 ggcgccatcc tgccgcagat cgcccctgaa aaggagctga cccggatcaa cgggctcaac
     1321 ggcagcatcc aggctgtcat catgctggtt tcgccgatga tcagtggcgc gctttaccaa
     1381 tttgcgccga tggaaggtat cttcctgatc gatatcgtga cagctgcttt ggcgatcgcc
     1441 atcctcgtgt tcttcctgaa agtccctacg catgaaaaag ccagccagga acaagtcagc
     1501 aattacctgc aggacatgaa gctaggcttc agctatatcc aaaatcatac ttttatcaaa
     1561 aggttgttcc tctatttttc gcttgctttc ttcatggcag caccggtgtc gttcctgtca
     1621 ccgctgcagg ttgcgcgctc attcggcgat gacgtctggc gcttgacggc gatcgaaatc
     1681 gccttttcga tcgggatgat cggcggcggc ctctggatcg cgtcatgggg cggattcaag
     1741 aaccggatcc attccatcgc ggccgcgatt gcagtaatgg gtttgtgctc tttcgggatg
     1801 ggcgtcatcc cgaacttctg gatttatctc ttcctgatgg gcttggtcgg acttttcatt
     1861 ccgctcctga acgctccgag catgacgctc ttgcaggaaa aggtcgaaga agatttcctc
     1921 ggacgcgtct tcggcgtcca aagcatggtc gcaagcgcaa tgatgccttt agggatgctg
     1981 gtattcgggc cgctggcgga ccggatggca atcgaaatcc tgatggccgt atcgggccta
     2041 ttcctgatgg tcgtggcctt cttcgccatc cgcgaccgtg tcctgcttga agcaggcaaa
     2101 cccgaacctt cggacaccgg aaaagaatca aactaaagag tggagctggc gtattcggca
     2161 gctctttttt gtggttgtcc gatactttgc gttaatcgga caagcagcat ccgcagcgca
     2221 agacgagcag caaagcatga atgtcggcaa accacaaaga agaacccgtc ctaaaatcgg
     2281 acgggttctt cttgagttaa tcagtttaca aaccgcattt cagctgtgcg ccgcagttgg
     2341 tacaggtgtt gcagccgccc agatcttcga ccgtcccttg acggcagatc gggcaagtgt
     2401 cgccgatttc gttgccgatt gtgacgtcgg tagcttcgag attggctatc gaatcaacca
     2461 ggaaagtctt gtccttgttg attttgacgt gctctttctc ttcctcgagc ttgccttcct
     2521 catcccagac attttcttcg gctttcagcg tcagtacctg ggaatcacgg ctgccgtcca
     2581 catagactgt tccgcctttg gcaccgccca agtaaaggcg ctcgtagatc ttttcgacct
     2641 gatcgacagt gtagcctttc ggtgcgttca ccgtttttga aatggagctg tcgacccagc
     2701 gttgaatcgt tgtctgcaca tcgacgtgct cttcgggcgc aagtgtcatg gcagccacga
     2761 agtaatcagg caagttcatc ggatcggcat ccggatgtcg atccagatat tcttggacga
     2821 tatccgcctt cacttcgatg aatttgccga gacgcccgct gcggtaatac gtgaacgaga
     2881 agtaaggttc gagtccggtg gaaacaccgg ccatcgtccc ggtcgatccg gtaggcgcca
     2941 ctgtcaacaa gtgcgagtta cggatgccat atttcaggac gccttcgcgg atgtgttcag
     3001 gcattttttt catgtagcct gtattgatga aacgttcgcg caggatctgc gtctctttac
     3061 cgctctgtcc gaccaggaac gggaagctgc cgcgcgtttt cgccaactcg atgctttcct
     3121 cgtaggccgc cacagcaatc gtttcgaata cttgatcgat cagctgcagg ctttccagtg
     3181 aaccgtaacg gacgccgcaa tagatcagca tgtcggccaa gcccatgatc cccaagccga
     3241 tccgacgttc gccaagcgct tgttttttgt tttcctccag gaagtaaggg gtggagtcga
     3301 tgacattgtc ctgcatatgg ataccggtgc ggacggtctg ctccaatttg gcgaaatcga
     3361 ccatctgcag gtccttgttc accatttcgg ccaagttgac tgccgctaag ttgcagacgg
     3421 aataggctgc caatggctgt tcgccgcaag gattggtcgc aacgactttt tgaccataag
     3481 ctgttgcgtt cgtcatcttg ttggcgttgt cgatgaagaa gatgccaggc tccgccgcat
     3541 aagtggcgca gacattgatc agccgccaca attcacgggc tttgacggtc cggtaggtgc
     3601 ggacagcatt gccggtagct tcccattcgc ggacatcgcc gcagttggtc cattcggcat
     3661 catagtgcgc catcgcgtcc gcgtcgtaat gttcgacatc cgggaaacgc aacgcatatt
     3721 cctcaccccg catcacggcg tccatgaagt cgtcggtgat gcagacggag atgttggcgc
     3781 cggtcaggaa ttcaggattg ttgacgctgt aggtgccccc atcgcgcaat ttcttctcgg
     3841 cttcatggat gaccgatgca tcaaatccgc catgccccgg gatttgtttg tagttgacga
     3901 tacctgtgta catgttgatt tctttaggtg aaaatggttt gaaatgcagc ttctccttcg
     3961 ccaacatgcg gatctgttca tcttcgaagt tttcgataat gtagcgaagg atgcgcggat
     4021 tctgcatttt cgaaatgatg aactcaaaga tatccggatg ccagtcggcc aacataatca
     4081 tctgggcacc acgacgggaa ccgccttgtt cgaccaaatg cgtcagcttg gcgatgtcat
     4141 ccatccagga gacggatccg gaagagcggc gattgacgcc ttttacgatt gtatggcgcg
     4201 ggcgcaacgt cgaaccgttc gaaccgacgc ctccgccacg gctcatgatt tccatgactt
     4261 ttttgcggtg atcggaaatg ccaccgcgcg agtcgggcac gaaaggcatg acgtaacagt
     4321 tgaaataggt gacatcggta cccgatccgg caccgtagag cacgcgtccg gccgggatga
     4381 aattcaaggc tttttgttct tcatagaatt tcaggaaagc ttcatggcgc agttccggcg
     4441 tgatttctac ctcagccaag ccatgagcat tacgcatcgc gatctgttcg tagaaaagct
     4501 ccagtggttt atccagcgtc aggatccggc gcgtcacgat gccgccttcc tcaacttcga
     4561 aatcatcgat gttgtgctgg tactccgcgg aaactctgat gcgtgccgtc tggtccttaa
     4621 ggttgatgga gagaatggtg ccggttcctc gagctgggta tttaggatcc tctttgaccg
     4681 ttaagataac cagatcccct tcggaaaggg tctcctttgt ggaatccttg taggagtaac
     4741 gatccagcat gacgaggcgg gacacgccct cataggtaat gtgcatatcc tccgtgatcg
     4801 gaaacacatg cgggaaccgc tcgatgtcct tgttcaattt ttcgaatcct gctttaaatg
     4861 tggtctttgt tgcatccaca ataatcactc cttcatttat ggcgaaaaca ctagatgttg
     4921 tgctgtcggt ttgtttctca taccatatat gttaattctg ttcgccagat aaatcaaggg
     4981 gaacaatcgg agagttgtat tttgaaaatg gatgaaaacg ttgtttgggt ggacgaggat
     5041 gggtttgtaa taatatatcg gccaataaga agaggacatt gtgcgtcgga aggggatgtc
     5101 ggctgatgca gagagaatga atgccgaaca gccgagacag ggcgcggatg tcgactgatc
     5161 gaataccgaa aatcgcaaaa aacccgacag ttcaccaatg tgtcggacgt cagcccaaga
     5221 ctgaatgcgg aatagtaccc ggtcagccat cccgaaagct ataataaaga cagaaagaac
     5281 aacatccgaa aggagcccaa gcatgaacga taaagaacgc ttcgcggata tgatccgcaa
     5341 atcaaaacgc atcgtcgcct tcacaggggc gggtatcagc acggagagcg ggatccctga
     5401 tttccgttcg gcgggcgggc tgttcgacag gctgagtggt cgccatttca ctggcgaaga
     5461 gacgctcagc atcggttttc tggagacgta tccgcaactt tttttccgta actatgcgga
     5521 gcacttcaat ttttcgcagg cgcgcccgaa cgcagggcat cgcttttttg cgcggcttga
     5581 gcatgcaggc aaagaagtca ccgtcgtcac gcagaacatc gacgggctgc accagaaggc
     5641 cgggaattca ccggcgaatg tgatcgaggt gcacggcacg gtgcgcgagt tcgtctcgga
     5701 gttccgcggc atcgccggca ccgcccgggt cgccggcgcg ctgcaggccg cgccctgagt
     5761 cacacagtga cacgatcggc gcggggtggg ggagtcgatg gc
//
LOCUS       DAOMUJ010000055         5796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3758_length_5796_cov_2.525692, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000055 DAOMUJ010000000
VERSION     DAOMUJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3758_length_5796_cov_2.525692"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..332)
                     /locus_tag="PLT73_02395"
     CDS             complement(117..332)
                     /locus_tag="PLT73_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19263.1"
                     /translation="MQKIYNYIEILTATLGSRFAKQPFRIRAKECALAFVTLLDGLDV
                     QLVYEDSQRYEELQAIVWDIFWKGISL"
     gene            620..862
                     /locus_tag="PLT73_02400"
     CDS             620..862
                     /locus_tag="PLT73_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRM19264.1"
                     /translation="MLQLPVKQWGNSHAIRLPKSVLEALEVEKDDELNVEIVNHSIVL
                     TKAEKDLTFQELFKGYDGEKFTSELQNLSPVGNEKW"
     gene            856..1194
                     /locus_tag="PLT73_02405"
     CDS             856..1194
                     /locus_tag="PLT73_02405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM19265.1"
                     /translation="MVKQGDIVKINLDPKQGHEQQGYRPYICLSYHGVSDYANVAVFA
                     PISNTERNYPLYVPLRGTESSGKVLLDQLVTIDYNARKYSYVETVPEKLIDELLMKVK
                     VIFQKNEKIV"
     gene            complement(1348..2199)
                     /locus_tag="PLT73_02410"
     CDS             complement(1348..2199)
                     /locus_tag="PLT73_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19266.1"
                     /translation="MNMTAFWDLGKVALKVNDLKKMTQFYQEAIGLDLMEEKENSVVL
                     GVAADNTPLVELIQLAAPSNRKRTTGLFHLAILLPTRADLGSILYHLLTKKYAIDGAS
                     DHGYSEAIYLNDPEGNGIEIYRDKEMSDWDIRPDGKIEGVTEAMDADGVIGAMVKIYD
                     GIPSGSIMGHVHLTVHDLVETQQFYQNVLGISLKSDFFGQAKFFAAGNYHHHIGSNMW
                     AGKQLPAAEKSQPGLAYFTWVVADNTAYEALKSRLVQAEVSFEEADNTLIFKDNSGIE
                     IHAVIHQ"
     gene            2481..4430
                     /gene="dnaX"
                     /locus_tag="PLT73_02415"
     CDS             2481..4430
                     /gene="dnaX"
                     /locus_tag="PLT73_02415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRM19267.1"
                     /translation="MSYQALYRVWRPQRFGDIAGQEAVTQTLKNALIQGKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCHHRVDGEPCNECETCRAITEGRLNDVIEIDAASNNGVE
                     EIRDIRDKARYAPTQADYKVYIIDEVHMLSTGAFNALLKTLEEPPENVIFILATTEPH
                     KIPLTIISRTQRFDFKRISYQDIINRMAYILQEEKIGYDEQALHVIARSANGGMRDAL
                     SLLDQIISYGSDAVTFENAVKVSGSLTEEMMISFSSALLHEETESALQLLQEILSAGK
                     EAGRFLEEMILFARDVLVYQQTKGKAFADNTQQYSEAFQTFSQEAPATFLYGMIEAFN
                     ETQGEMRFSTQPDIYLEVLAVKLSQSKAHAHATAAVPSAQAAPNGEVQRLAHELELVK
                     KELAKLQELIASGNLTVSQEGAKAAPAKKETARQSNTSAGFKPNMGRTYQILGEATKQ
                     DLARVRDFWTDILDVLSVTQRAVLKQANPVAASPTSFILSFQYDILCQKATEDAELQA
                     AVDAATQRMLQRPGELVCLTEEQWTSVRRNYIQNRNGSPAAESNGELKQAAPKQAPVL
                     SAAPKQPASATGDKDKPVDLDLPPEPPMPEMEDGYRGDYHPGNFADDFSVETDPIVIE
                     EQKEQEVVDQALSLFGGEVVTVVED"
     gene            4498..4812
                     /locus_tag="PLT73_02420"
     CDS             4498..4812
                     /locus_tag="PLT73_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRM19268.1"
                     /translation="MRGGMGNMQGMMKQMQKMQKELEQTQAQLNVTEFVGAASNDLVK
                     ITMTGDKKVTDVVIAPEVVDPEDVEMLQDLILMATNDALSKIDEATKAKLGKFANAIP
                     GF"
     gene            4931..5527
                     /gene="recR"
                     /locus_tag="PLT73_02425"
     CDS             4931..5527
                     /gene="recR"
                     /locus_tag="PLT73_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRM19269.1"
                     /translation="MQYPEPIAKLIESFMKLPGIGQKTAARLAFFCLDMDEEAVLAFA
                     DALIRSKRDLHQCAICGNITETDPCNICSDTTRDASIILVVENPRDVMSMEKIREYHG
                     RYHVLNGVLSPMEGTGPEDLNIASLIKRLQDETVKEIIIATNATAEGEATAMYLSRLI
                     KPAGIKVTRLAYGLAVGSDIEYADEMTLFRAIDGRREL"
     gene            5563..>5796
                     /locus_tag="PLT73_02430"
     CDS             5563..>5796
                     /locus_tag="PLT73_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HRM19270.1"
                     /translation="MAFHLNSASKKQGHLKQQYDEKLIDLIRDIHQHYTTLAQLQQNA
                     YDLPEELRIFEKIAEAKYLFLLREAKERQIQADI"
BASE COUNT         1642 a         1316 c         1449 g         1389 t
ORIGIN      
        1 taagacgcta atcctgtagg gcagggttag cgtcttagtt tttttagatg atcgcgttca
       61 tagcttagga gtatcagtgg aatatctgct gattggagtg gctgttttcc ccagtatcac
      121 agactgatac ctttccagaa gatatcccag actattgctt gtaattcttc atacctttgg
      181 ctatcttcat agactaattg aacgtccagg ccatccaaca atgtgacaaa agctagcgca
      241 cactctttcg ctctgatacg aaatggttgt tttgcaaagc gagatcctaa cgttgcagtc
      301 aatatctcta tataattata gattttttgc ataacatctt catgcatctc atccggtaca
      361 agaaaagaaa tagtcagcat cagttttgtg ttcggattcg tcagaaagcg ttccttatgt
      421 tccgttaaga actgataaag ctgctgctca atcgattcct ctccatgttg ctgaaattac
      481 tgctgaataa attgtagctc gtcgtctacc gcttctgtat aaacttcttc cattgctcgt
      541 aattaaaaaa ggcaagtaga tgtgtataca aagtagatac tttggtggta agatgaactc
      601 aaagaaaggg ggatttatga tgctgcaatt acctgtcaaa caatggggga acagtcacgc
      661 aatacgcttg ccaaaaagtg tattggaagc actggaagta gagaaagatg atgaactgaa
      721 tgtagaaatt gtcaatcatt cgattgtgtt gaccaaagcc gaaaaggacc tgacttttca
      781 agaactgttc aaagggtacg acggagaaaa attcacttca gaattacaaa acctttcgcc
      841 agtggggaat gagaaatggt aaaacaagga gatattgtta agattaattt agacccaaaa
      901 caagggcacg agcagcaagg atacagaccg tatatctgtt tgagctatca tggggtcagt
      961 gactatgcta atgtggctgt attcgctccc atttcaaata cagaacgcaa ttatcctttg
     1021 tatgtgccat taagaggcac agagtcttca ggaaaagttt tgctggatca actagtaaca
     1081 atcgattaca acgcgaggaa gtatagctat gttgaaaccg taccagaaaa actaatcgat
     1141 gaattgctga tgaaagtaaa agtaatcttt caaaaaaacg aaaaaattgt ttgatgaatc
     1201 tcaatgcgta cctctcacac ttcccgtaac ctagggtata ctcgaaaaac tttttgattt
     1261 ggtatttttt tcgactatat ccaaaaaaag cgcaaccggc aggaatatcc gccggtcgcg
     1321 cttttttgga tgataaataa atgactatta ttgatgaatg accgcatgga tctcgatgcc
     1381 gctgttgtct ttgaaaataa gggtgttgtc cgcttcttca aacgaaactt ctgcctgaac
     1441 cagtctgctt ttcagcgctt cataggcagt gttgtcggca actacccaag tgaagtaggc
     1501 caaaccgggt tggctctttt cagcggcggg taactgtttt ccagcccaca tattcgaacc
     1561 gatgtgatgg tgatagtttc cggcggcgaa gaattttgct tgcccgaaga agtctgactt
     1621 caatgaaata ccgagtacgt tttggtaaaa ttgttgcgtt tcgaccagat cgtgaacggt
     1681 caaatggacg tgtcccatta tggagccgga agggatgcca tcataaattt tgaccatcgc
     1741 gccgattacc ccatcagcgt ccatcgcttc ggtaacgcct tcgattttgc catccggacg
     1801 gatatcccaa tccgacatct ctttgtcgcg gtagatttcg atgccattgc cttctggatc
     1861 gtttaagtaa atcgcttcgc tgtagccgtg gtcactcgct ccatcaatgg catatttttt
     1921 cgtcaacaaa tgataaagga tggatcccaa atccgcgcga gtcggtaaaa gaatcgccaa
     1981 gtggaaaagt ccggttgtcc gtttgcggtt ggagggggca gcgagttgga tcagctccac
     2041 taaaggcgtg ttgtccgcag caacccccaa cacaaccgaa ttttcctttt cttccattaa
     2101 atccaagcca attgcttcct gataaaattg cgtcattttc ttaagatcat tgactttcaa
     2161 ggcaaccttg cccaaatccc aaaatgctgt catgttcatt ttccccttta ttatcgtttt
     2221 tatttgaatt ttattttttc cggaataata tcacaaattt aattaatacg aattcgaaat
     2281 acttgatgga tttactataa tgcctaactt actttttgtc aatcattatt atttaattct
     2341 tttatttgag ctgaatcgcc ttctaaagaa agcaaaatac aagcttatat tccacccgaa
     2401 agccatagag ccgcgccttc ctattttcct gcttttccct tataatagat ggacgaagca
     2461 aaagtgaaag agggaaaagg atgagctatc aagcactata tcgggtttgg cgcccgcagc
     2521 ggttcggaga catcgcaggt caggaagcgg tcacgcagac gttgaagaat gccttaatac
     2581 aggggaaaac gagccatgct taccttttta ccgggccgcg gggaacgggt aagacgagtg
     2641 cagccaagat ttttgcgaaa gccatcaact gccatcatcg cgtcgacggg gagccgtgca
     2701 acgaatgcga aacgtgccgt gccatcaccg agggacgctt gaacgatgtc atcgaaatcg
     2761 atgccgccag caacaacggt gtggaggaaa tccgggatat ccgcgacaaa gcgcgttatg
     2821 ccccgaccca agccgactat aaagtctaca ttatcgatga ggtgcacatg ctctcgactg
     2881 gtgccttcaa cgcgcttctg aagacgctgg aggagccgcc ggaaaatgtc atcttcattt
     2941 tggcgacgac ggagccgcac aagatcccgt tgacgatcat ttccagaacg caacggtttg
     3001 acttcaaacg tatttcctat caggacatca tcaaccggat ggcctacatc ctgcaggagg
     3061 agaagatcgg ctacgatgag caggcgctgc atgtcatcgc gcgctccgcg aacggcggga
     3121 tgcgggatgc actcagcttg ctcgatcaga tcatttctta cggatcggat gcggtcactt
     3181 tcgaaaatgc ggtgaaagtt tccggcagtc tgacagaaga aatgatgatt tccttctcaa
     3241 gcgcgttgct gcatgaagag acggaatcgg cactgcaact gctgcaggaa atcctgagtg
     3301 caggcaaaga ggctggccgt ttccttgagg agatgatcct gtttgcgcgg gatgtgctgg
     3361 tctaccagca aacaaaaggc aaggcctttg ccgacaatac gcaacaatac agtgaagctt
     3421 tccagacttt ttcgcaagag gcgccggcaa cgttcctcta cgggatgatt gaggccttca
     3481 acgagaccca aggcgagatg cgcttctcga cgcaaccgga tatctatctg gaagtgttgg
     3541 cggtgaagct gtctcaatcc aaggcgcatg ctcatgcaac ggccgcagtt ccgagcgcac
     3601 aagccgcgcc gaacggagaa gttcagcggc tggcgcacga gttggagctc gtcaaaaagg
     3661 aactcgcgaa gctgcaggaa ctgatcgcct ccgggaatct gactgtttcg caggaaggtg
     3721 ccaaagcggc gcctgccaag aaagaaacgg ctcgtcagtc gaacacgtct gccggattca
     3781 agcccaacat ggggcggacg taccagattt tgggtgaggc gacaaagcaa gatcttgccc
     3841 gcgtccgtga tttctggacg gatatcctgg atgttctctc ggttacccag cgtgctgtcc
     3901 tgaagcaagc caatccggtg gcagcgagtc cgaccagttt catcctgtcg ttccagtatg
     3961 atatcctttg ccaaaaagcg acggaagatg cggaactgca ggcggctgtg gatgcagcaa
     4021 cgcagcggat gctgcagcgt cccggcgaat tggtgtgttt gacggaagag caatggacat
     4081 cggtgcgacg caactacatc cagaaccgga acggcagccc tgctgccgaa tccaatggcg
     4141 aactgaaaca agccgcgccg aagcaggctc cggtactatc ggctgctccg aaacagcctg
     4201 cttcggcaac tggcgataaa gataaacctg tcgatctgga tttgccgccg gaaccaccga
     4261 tgccggaaat ggaagatggc tatcgcggtg actatcaccc cggaaatttc gccgatgatt
     4321 tttcggtcga gaccgatcca atcgtgatag aggaacaaaa ggaacaagaa gtcgtcgatc
     4381 aggcgttgag ccttttcgga ggcgaagttg taacggtcgt cgaggactga cggcgttcga
     4441 aataaacaca agctatgaac acaaactaaa atacacaaac agagagggag tcatacaatg
     4501 cgaggcggaa tgggaaatat gcaaggcatg atgaaacaaa tgcaaaaaat gcaaaaagaa
     4561 ttggagcaga cgcaagcaca actgaacgta acggagtttg tcggcgcggc atccaatgat
     4621 ctggtcaaaa tcacgatgac aggggacaaa aaagtcactg atgttgtcat cgcacccgaa
     4681 gtggtcgacc cggaagatgt ggaaatgctg caggatctga tcctgatggc gacgaatgac
     4741 gcattaagca agattgatga agcaacgaaa gcgaaattgg gcaaatttgc caatgcgatc
     4801 ccaggcttct aagccaggcc gcaagtaata accaaaatga atgaggggag cgagggtgag
     4861 acaagtcggg cagtgttgac tttagtccca gcctcgtttc ttttgagaga agagaagggg
     4921 atttttcgag atgcaatatc cagaacccat agctaaatta atcgaaagtt tcatgaaatt
     4981 gcctggcatc ggacaaaaaa cggccgcccg attggctttt ttctgtctgg acatggatga
     5041 ggaggctgtg ctcgcgttcg cggacgcgct gatccgttca aaaagggatt tacaccaatg
     5101 tgccatctgc ggtaatatta ccgaaacaga tccgtgcaat atctgttcgg acacgacgcg
     5161 agacgcgtcc atcatcctgg tggtcgaaaa tccccgcgat gtcatgtcta tggagaaaat
     5221 ccgtgagtac cacggtcgat accatgtgtt gaacggggtg ctttcgccga tggaggggac
     5281 tggacctgaa gatctgaaca tcgccagcct gataaaacgg ctacaggacg aaacagtcaa
     5341 ggagatcatc atcgcgacaa atgcgacagc cgaaggggaa gcgacggcga tgtacctttc
     5401 gcgcttgatc aaaccggcgg gcatcaaagt gacgcgtctt gcctacggac tggctgtcgg
     5461 cagcgatatc gaatatgcgg atgagatgac gctctttcgt gccatcgatg gacgccgaga
     5521 actgtaacaa aggcagcaaa aacgggggga acaaggaaaa agatggcatt ccatttaaac
     5581 agcgcaagca agaagcaggg acacttaaag cagcaatacg acgaaaagtt gattgatttg
     5641 atccgcgaca tccatcaaca ttacacgacc ttggcccaac tgcaacagaa tgcctatgat
     5701 ttgcctgaag agttgcgtat ttttgagaag attgcggaag cgaaatatct gttcctgctg
     5761 cgggaagcca aagaaagaca gatccaagcg gatatt
//
LOCUS       DAOMUJ010000056         5741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3813_length_5741_cov_2.136124, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000056 DAOMUJ010000000
VERSION     DAOMUJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_5741_cov_2.136124"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLT73_02435"
     CDS             complement(<1..471)
                     /locus_tag="PLT73_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRM19271.1"
                     /translation="MNSKKKLYGSAALLSLLLLVGCSADEDTATNDSSAVGSSEMIES
                     SSEQDESMMEDSAEGDMEESESLETPYIYGAVGALADNNLTMEEMFTYAIQDEHLAHE
                     EYAYVLETFGDQAPFNNIISSEAQHITEMTVLFEKYNLAIPADESADHIQRAADV"
     gene            807..1628
                     /locus_tag="PLT73_02440"
     CDS             807..1628
                     /locus_tag="PLT73_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRM19272.1"
                     /translation="METKAETRQLETPIKITVAVTYLIMVIVNALANILPINGIDTGA
                     ISDSYPNLFAPAGLTFSIWGVIYLLLLGYTLYQFGLFQGDKSKVKTELLRKIGIVFSA
                     SSVVNAAWIFSWHYRMIGLSVILMLVILLSLIYINQLILKEKLDQKEKLFIRLPFSVY
                     FGWITVATIANITTLLVDIGWNGFGISESIWTILIIVTGLVIGAVTMIRNHDIAYGLV
                     IIWAYAGILIKHMSQDGFAGQYTGIIMTVIACIVLMGVAIGYVLFAKKQVPTSLK"
     gene            1823..2281
                     /locus_tag="PLT73_02445"
     CDS             1823..2281
                     /locus_tag="PLT73_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRM19273.1"
                     /translation="MSFVIEMVETQEQPTLVLKTVTPVSELPKILGKAFMDIVTHIIE
                     LGEQPVGPAFVGYFNMDMERLELEIGFPVSKPLPGKGDILAGNIPAGKQVSCIYKGPY
                     MEMPSAYEEIEKWIKDNGYKPLGPVYEHYYNSPEEVPESELLTKIVFLVE"
     gene            2322..2456
                     /locus_tag="PLT73_02450"
     CDS             2322..2456
                     /locus_tag="PLT73_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19274.1"
                     /translation="MTDVMINKEVEVFFTGKPETRALFRAVERKIRAIGPAIITVTKT
                     "
     gene            2457..2732
                     /locus_tag="PLT73_02455"
     CDS             2457..2732
                     /locus_tag="PLT73_02455"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRM19275.1"
                     /translation="MSFATRTQFAWIWMPQPTDRKRPLHSLVLSFGCGRQIVHDQIVE
                     AVEPYPGRWTHHVIIAEEADLTDVLDTWLQEAYRFSETRGRQQSSKI"
     gene            2860..3606
                     /locus_tag="PLT73_02460"
     CDS             2860..3606
                     /locus_tag="PLT73_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19276.1"
                     /translation="MASGRRSSHYTLVDLRNLCIPTTPEIKKALALILATSIGPDGGI
                     NPSKDIEQSDVCINGMFLNYGCYFGTDEEKLRSVVDFIVSQQLADGGFNCRLNRSGAR
                     HSSMHSTISVLEGIREYIAAGYAFRAEELNRIEGEAQEFLLRHRLFKSDHTGAVIHPS
                     FLKLVYPPRWRYDILRALDYFQSVGYPYDERLQDALDILKEKRLSDGRWKTQAHHPGE
                     VHFQMEQAGKPSRWNTLRCLRVLEYYDRDA"
     gene            3656..4249
                     /locus_tag="PLT73_02465"
     CDS             3656..4249
                     /locus_tag="PLT73_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRM19277.1"
                     /translation="MTESGFPEEWLSLRNPQEWHDWLVQKHDQKDQIWLKIKKSHTKG
                     EGVLLAEAVEEALCFGWIDGKMYSLDKESFIIRMTPRRPGSVWSLVNRKRAEALMAAG
                     RMTEAGLAAIQAAKTNGKWQAAYSSKEVPELPEELEQAFKDDPLARACFEGWPTGEKA
                     HYLFWIAHAKRPDTRKKRIAETLERAQAKKKPSPLRG"
     gene            complement(4273..5268)
                     /locus_tag="PLT73_02470"
     CDS             complement(4273..5268)
                     /locus_tag="PLT73_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HRM19278.1"
                     /translation="MKLSILDQVHITAGGNAEQSLQNAKELARLGDSLGYERIWFAEH
                     HGSNLMASAAPEIIAAFIAAATDRIRVGTGGVMMMHYSPLKIAEVFKTLSGFAPGRID
                     LGVGRAPGGDRQSMYALAQGNPPQLENHYEKLRTILDLLEDRKPQDPLYRDTPAAPVD
                     VTVPQTWLLGSSGNSAVQAGRMGVGYSFAQFFTGTLDKSIFDLYKKNFAPSAAMPEKQ
                     ISVAFHAIVADTREEALYEELPYAIFKMQLFRGMMRNHLMTPEQAAAYPLTEIEKQLI
                     NDIRKTHIIGTAKEAGETLLNLQEQYGFDEAMIISMPHSQKARLKTYSLLAQELL"
     gene            5424..>5741
                     /locus_tag="PLT73_02475"
     CDS             5424..>5741
                     /locus_tag="PLT73_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19279.1"
                     /translation="MSLIHHIEINVSDLTASKQLWSWLLEKLGFSKYQEWEQGFSYKE
                     DDTYIVFVQTAERFLDGSYHRGRTGLNHLAFAVDTKHQVDELYKELQSRGIPLLYTER
                     HPFA"
BASE COUNT         1482 a         1316 c         1531 g         1412 t
ORIGIN      
        1 tacatccgcc gcacgttgga tgtgatcggc tgattcgtca gccggaatcg ccaaattgta
       61 tttttcgaac agaacagtca tttcggtgat gtgctgagct tcagaagaaa tgatgttgtt
      121 gaacggagct tgatctccga aggtttccag tacgtaggcg tattcttcgt gggccaagtg
      181 ttcatcttgg atggcgtacg tgaacatctc ttccatcgtc agattgttgt ccgccaaggc
      241 gccaactgca ccatagatat agggtgtttc aagcgattcg ctttcttcca tgtcgccttc
      301 tgcgctgtct tccatcatgg attcatcttg ttcggaacta ctttcgatca tttccgatga
      361 gccgactgct gaagaatcat ttgtcgcggt atcttcgtcc gcggaacaac cgaccaacag
      421 taatagactc aacaacgcgg cggaaccgta gagtttcttc ttgctgttca ttttttttcg
      481 tcctttcata caaacgctgc acgattgaat ggggaagggg atttgagaag ttttccgacg
      541 ttgttgaatg atgaatggga aggatgaacg aagaatgaag aacttgtcac cattaaggta
      601 cgcctcactc aaaaaaatgt caaaaaattg gacctgttcg gaagaaaaat taacctctcc
      661 cacgaaaccg caatatttgc ataggttcac tattttaagt attattgatt tagaataata
      721 atttatttat agtgaataat tttgtgcatc aagctatcta catgcaatta tttttatcag
      781 gaaaaatgga agaggaggaa acaaatatgg aaacaaaagc agaaacgaga caactggaaa
      841 cacccatcaa aatcactgtc gctgtgacgt atctgattat ggttatcgtc aacgcattgg
      901 caaacatcct tccgatcaac gggatcgata caggtgcgat atcggattcc tatccgaacc
      961 tgtttgcgcc tgctggattg acgttttcga tttggggcgt catctatttg ttgctgttgg
     1021 gctatacgct ttaccaattc ggtttgttcc aaggggataa gagcaaggtg aagacggaat
     1081 tgttgcgtaa aatcggtatc gtcttttcgg ccagttcagt agtcaacgcc gcttggatct
     1141 tcagttggca ttaccgcatg atcggcctgt cggtgatctt gatgctggtg attctgctta
     1201 gcctgatcta catcaaccag ttgatcctga aggaaaaact cgatcagaaa gaaaaactgt
     1261 tcatccgtct tccgttcagc gtctatttcg gctggatcac ggtggcaacg attgcgaaca
     1321 tcacgacgct gctggtcgac atcggctgga atggcttcgg aatttctgag tcgatttgga
     1381 caatactgat catcgtaacc gggcttgtga tcggagccgt cacgatgatc cgcaaccatg
     1441 acatcgccta cgggctggtg atcatctggg cttatgcggg catcctgatc aagcacatgt
     1501 cccaggacgg ctttgccggg caatacaccg gcatcatcat gaccgtcatc gcttgtatcg
     1561 tactgatggg cgtggcgatc ggctacgtgc ttttcgcgaa gaaacaagtg ccgaccagct
     1621 tgaaatgatg acaattttga taaagttata gtttgattga cttgaaacag gtcccggcaa
     1681 cgattcgact gaattggcgc cgggacttgt ttttgtgcca tttcagaccc tttatgcggc
     1741 tatcgtattg atggtcgtgc ggatgcggaa gtgataaaat aagcatgaga caatgggctt
     1801 caaaaaagga gacgtgtata aaatgagctt tgtgattgaa atggtggaaa cacaggaaca
     1861 accgactttg gttttgaaaa cggttactcc ggtaagtgag cttccgaaaa tattggggaa
     1921 ggccttcatg gacatcgtca cgcacatcat tgaattgggt gaacaaccgg ttggaccagc
     1981 tttcgtcggc tatttcaaca tggatatgga aaggctcgaa ctggaaatcg gttttcctgt
     2041 atccaaacca ctgcctggca aaggcgatat ccttgcagga aacatccctg ccggcaaaca
     2101 ggtcagctgc atatacaaag gtccttatat ggagatgccg tctgcttatg aggaaattga
     2161 gaaatggatc aaagataatg gctacaaacc gcttggtccc gtctatgagc attattacaa
     2221 ttcgcctgag gaagtacccg aaagcgaact gctgacgaaa attgtattcc tggtggagtg
     2281 agcatacgcc aattcctatt tcaaaaaatg aggcgatacc tatgacagat gtaatgatca
     2341 acaaagaagt ggaagtattt tttaccggaa aacctgaaac gagagcattg ttcagggctg
     2401 tggaaaggaa aatccgggca atcggacccg ccatcatcac ggtgacaaag acctagattt
     2461 ccttcgctac aagaacgcaa ttcgcctgga tctggatgcc gcagcctaca gacagaaagc
     2521 gaccgctgca cagtttggtg ctgagcttcg gctgcgggcg ccaaattgtg catgatcaga
     2581 ttgtcgaagc ggtcgaaccg taccccggca gatggacgca ccacgtcatc attgcggagg
     2641 aagctgatct gacggatgtc ttggatactt ggctacagga agcgtaccgg ttttcggaaa
     2701 cacgggggcg acagcaatct tcaaagatat aggcaactga acagcaaccg ctgtggatca
     2761 gttctgtcga caaaataagt gaagttgatc gaacggaaac tgcggaagta ggttgaaaaa
     2821 ggaagggccg atggaaatga atagggcgga aatcatcgcg tggcttctgg aaggcgatcc
     2881 agccattaca ccttggtgga tctgcgcaac ctctgcatcc cgacaacacc ggaaatcaag
     2941 aaggcgttgg cgctcatcct tgccacttca atcgggcctg atggtggcat taatccgtca
     3001 aaggatatcg agcagagtga tgtctgcatc aacgggatgt tcctgaacta tggttgttat
     3061 ttcgggacgg atgaggaaaa actcaggtcg gtggtcgatt tcatcgttag tcagcagctg
     3121 gccgatggcg gcttcaattg tcgcctgaac cgatctggtg cccgtcacag ttcgatgcat
     3181 tccacgatca gcgtcctgga gggcatccgt gagtacatcg ctgccggtta tgccttcagg
     3241 gcggaagagt tgaatcggat tgaaggagag gcacaggaat tccttctgcg gcatcggctg
     3301 tttaaatccg accataccgg cgcagtcatc catccgagct ttttgaaatt ggtatacccg
     3361 ccgcgttggc gctatgatat tttgcgcgcg ctcgattatt ttcaaagtgt cggttatccg
     3421 tatgatgaaa ggctgcagga tgctttggac attctgaagg aaaagcgact gtcggacgga
     3481 cgctggaaaa cgcaggccca tcatcccggc gaagtgcatt ttcagatgga acaggccgga
     3541 aagccaagcc gctggaatac gctcagatgc ctgcgagtac tggaatatta tgacagggat
     3601 gcatgatgct tttaaagagc atcatgcatc ccctgaaatg gaaggagagt tagttatgac
     3661 ggaatccgga tttcccgagg agtggctcag tttgcgtaat cctcaggaat ggcatgactg
     3721 gctggtgcaa aagcatgatc aaaaggatca gatttggcta aaaatcaaaa aatcccacac
     3781 taaaggcgag ggtgtactgc tcgcagaagc ggtcgaagag gcgctctgtt tcggctggat
     3841 cgacggtaag atgtacagtc tggataagga aagcttcatc atccgcatga ctcctcgcag
     3901 gccagggagc gtgtggtcgc tggtcaaccg caaacgtgcc gaagctttga tggcggcagg
     3961 aaggatgact gaagccggtt tggcggcaat ccaagcagcg aagaccaacg gaaaatggca
     4021 agcagcgtat tcctctaagg aagtcccgga gctgcccgag gaattggagc aggcattcaa
     4081 ggatgatccg ttagcccgcg cgtgtttcga aggctggccg accggtgaaa aggcccacta
     4141 tcttttctgg atcgcacacg caaaacgtcc cgatacccgg aaaaaaagga ttgccgaaac
     4201 gttggaacgg gcacaagcca aaaaaaagcc atccccttta agaggatgac cgagcttgat
     4261 gcggtagaca gtctacagca gttcctgcgc caacaaggaa taggttttga gccgcgcctt
     4321 ttgggaatgc ggcatgctga tgatcatcgc ttcgtcgaaa ccgtactgtt cctgcagatt
     4381 cagcaatgtt tctccggctt ctttcgcggt cccgataata tgcgtcttgc ggatgtcgtt
     4441 gataagctgc ttctcgattt ccgtcagcgg gtaagcggcg gcttgttccg gggtcatcag
     4501 gtggttgcgc atcattccgc ggaaaagctg catcttgaag attgcatacg gcaactcttc
     4561 gtagagcgct tcttcgcgcg tatccgcgac gatggcatgg aaagcgacgc taatctgttt
     4621 ttcgggcatg gctgcggatg gggcaaaatt cttcttgtag aggtcgaaaa tacttttatc
     4681 gagcgtcccg gtgaagaact gcgcgaatga atagccgacg cccattctgc cggcctggac
     4741 ggcactgttt ccgctcgaac ccaataacca ggtctgcgga acggtcacgt ctacaggcgc
     4801 cgcgggagtg tcccggtaaa gtggatcctg gggtttccgg tcttccaaaa gatccaatat
     4861 cgtccgcagt ttttcgtaat gattctccaa ttggggcggg ttgccttgcg ctaatgcgta
     4921 catggactga cgatcgcctc cgggggcacg gcctacgccc aaatcgatcc gaccgggtgc
     4981 aaagccggac agtgttttga agacttccgc gattttcaaa ggggagtaat gcatcatcat
     5041 gacgcctccg gttccgacac ggatccgatc cgtcgcggcc gcgatgaagg cggcgatgat
     5101 ttcgggcgcg gcgctcgcca tcaaattgct gccgtgatgc tcggcgaacc aaatgcgttc
     5161 gtacccgagg ctatcgccta aacgggcgag ttctttggcg ttctgcaggg actgctcggc
     5221 gttgccgcct gctgtgatgt ggacttggtc caagatgctg agtttcataa tttcctccga
     5281 ttctttattc tgaatacatt tgtgcaaatg atttccaaat tattgataga ttgactataa
     5341 caaacccagc cgcttgggac aaagaattgg cttttgaatt gtaagcggga tagggtggtc
     5401 acaaaagaaa aggaagtggg taaatgagcc tcattcatca tatcgaaatc aatgtcagcg
     5461 atctgacggc cagcaagcaa ttgtggtcgt ggctgctgga aaagctgggc ttcagcaaat
     5521 accaggaatg ggagcaaggc ttcagctaca aggaagatga tacatacatc gtcttcgtcc
     5581 agacagccga acgtttcctg gatggatcct atcaccgcgg acggaccgga ttgaaccacc
     5641 tggccttcgc tgtagacaca aagcatcaag tggacgaact gtacaaagaa ctgcaaagtc
     5701 gcggcatccc gttgctgtat accgaaagac atcctttcgc a
//
LOCUS       DAOMUJ010000057         5740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3815_length_5740_cov_2.916095, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000057 DAOMUJ010000000
VERSION     DAOMUJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_5740_cov_2.916095"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="PLT73_02480"
     CDS             <1..1776
                     /locus_tag="PLT73_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HRM19280.1"
                     /translation="KGMGRSISRYPLTVVFLIAVAVLNSFMIENPREDYARYLFTFLV
                     GAMLSIVGQMIYERFFTQMNARYLLMGGAVLLATGYYFAVGPQTLYNIAISVKTGVAL
                     FALMIAFIWIPSIENIKVPFHRSFLSALKAFFTTILFSGVLAAGISAIFYATDYLLFN
                     IDYKMLTHLLNIVGSLFAPIYFLSMTPLYPGKREEMIPDEAWAKRGETLERQFMVPRF
                     LEVLISYIIIPLTAIYTLILLVYVVMNIRGEFWTDNLLEPLLVSYAIIVIIVYILACN
                     LENKFADLFRKIFPKVLIPIVVFQTIASFLKIREMGITHGRYYVILFGIFATIAGVIF
                     SFMKPKHNGLIAIALLVLSAISIIPPIDAFTVSKNNQIGFLERKLIENDMLVDNAIVP
                     KSDVPLGDKIVITKVASYIDNMGYNKEVAYLPSDFNVYSDFTDTYGFDMTYSETDYPQ
                     GEGEFVYLNWDADPVVGIAGFDVMLHQYLYYSEVETSPVETTDFEVDGQPYQLEKRLD
                     GTYYILTVKDAAGEELIAYNTREVYDTIFEQSETSGFDKGNLTIEDATITTENDQIRM
                     NILVNSLERTPDFTGGDLYLFIEFK"
     gene            1944..2834
                     /locus_tag="PLT73_02485"
     CDS             1944..2834
                     /locus_tag="PLT73_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM19281.1"
                     /translation="MSKGKGKVMLSMLIFGTISIFVKNIDLASNQVAFFRGALGSLFL
                     WGTLMVRKEKIPLALVRKNAKFLLLSGFAVGMNWILLFEAFRYTTVSNATLSYYFQPI
                     FMTLLAPFVFKESLTAKKIACVLLAMLGMFLIIGVSPNAGNYNHPVGIGFGLAAAFFY
                     AVAIMSNKKIVGLGGIPLTALTLVIATGILTPYVALTSGFGLGQLTGGSITSLLVLGI
                     IHTGIAYVLMFAGAQVVESQTFAVLSYVDPVTAIVVSAVFLKEDLSPVQLAGGVLILA
                     AAFLNEFLGNRDKQIALSKE"
     gene            complement(3204..4058)
                     /locus_tag="PLT73_02490"
     CDS             complement(3204..4058)
                     /locus_tag="PLT73_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM19282.1"
                     /translation="MNIFSKMDNLVSLTENEKILVSYIKENPEAFVKMTSAEISEASF
                     ISNATIYRLCKKLDLAGLSELKILVSASVGDYLKERKTLDFNYPIHPNETQYQITRTM
                     QELYEQTVVSSLNNLDLEQLRQAASLMKKVPSIDLYTSAGNLFFAENFKFQMQEIGRF
                     VNVPIEEYHQRLTAAASDPSHLSIVVSYGGRGTVIPEIVKILKNNRSPILLITSTDAI
                     PFAGAATHHLYMSSNESHYNKISSFSTRLSLLFLLDCLYTSYFKLDYDHNVAMKMDYY
                     KKISRITW"
     gene            complement(4070..4993)
                     /locus_tag="PLT73_02495"
     CDS             complement(4070..4993)
                     /locus_tag="PLT73_02495"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRM19283.1"
                     /translation="MDYVAIDFETANSRGDSVCSIGLSRFSDGKEVDRYYALINPRQY
                     FSRGNIQIHGITEDMVAREPDFAGLYPDIRSFIGTDPLVAHFAQFDMSCLQQTIETYK
                     LPKMQNEFFCSCKMAQRMLDLHNNRLPTVLDYFGMTIDNHHNAVEDAVACGLITSKML
                     AQYDYDIQGFLDEYQYRMGKLFSHAFGVAKGGKSTPARRTKTAAPLKPKSLLFDREHV
                     FYDKHVCFTGRFQKLKRNDLAQLVVDVGGHFDANLSYETLYLVVADSDWRKIGTPSES
                     RKIQAVRELQGSGHNLTMLSESDFLRNFLKE"
     gene            complement(5043..5333)
                     /locus_tag="PLT73_02500"
     CDS             complement(5043..5333)
                     /locus_tag="PLT73_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19284.1"
                     /translation="MTEKMKQRLLLVFATVVGFVIGYLNPATSQALLSGIGWIAGIGM
                     FILFRRSNKNPEHDYSESWAYLLIRMLLFFIIGAALGSMIPYYQQIMALQQQ"
BASE COUNT         1398 a         1361 c         1440 g         1541 t
ORIGIN      
        1 aaaggaatgg ggcgttccat cagtcgttat ccactgaccg tcgttttttt gattgcggtc
       61 gcagtattga acagcttcat gatcgaaaat ccgcgcgagg attatgcccg ctatctgttc
      121 acttttttgg tgggggccat gctgagcatc gttggccaaa tgatttacga aaggttcttt
      181 acgcaaatga acgcccgcta tctgctgatg ggcggagcgg tattacttgc aaccggctat
      241 tacttcgcgg taggaccgca gacactgtat aacatcgcca tcagcgtcaa aaccggtgtg
      301 gcgctgttcg cccttatgat tgccttcatt tggatacctt cgattgaaaa tatcaaagtg
      361 cctttccacc ggagtttttt gtcagcgctg aaagccttct ttacgaccat tcttttttca
      421 ggagtgttgg cagcaggtat cagcgccatt ttttacgcta cggattacct tctcttcaat
      481 atcgattata aaatgctcac acatcttctg aacatagtcg gttcattgtt cgcgccgatc
      541 tatttcttgt cgatgacacc actgtacccg ggtaaacgtg aagaaatgat accggatgag
      601 gcgtgggcga aacgcggcga aacgctggaa cgtcaattta tggtaccgcg cttcctggaa
      661 gttctgattt cctacatcat cattcctttg acggccattt acacgttgat tctcttggtc
      721 tatgtcgtca tgaacatccg cggagaattc tggacagaca atcttttgga gccgctgctt
      781 gtttcctatg cgatcatcgt catcatcgtc tatatcctcg cctgcaatct cgagaataaa
      841 ttcgctgatc tgttccgcaa aattttccct aaggtcctga ttccgattgt cgtcttccag
      901 accatcgctt ccttcttgaa aatcagggag atgggtatca cgcatggccg ttattacgtc
      961 atcctgttcg ggattttcgc tacgatagcc ggggtaatct tcagtttcat gaaaccgaag
     1021 cacaacggat tgatcgccat cgccttactg gtattgtcgg ctatttccat cattccgcca
     1081 atcgatgcct ttaccgtcag caagaataac cagatcggtt ttttggaacg gaaattgatc
     1141 gaaaatgata tgttggtgga taacgcaatc gtgccaaaaa gcgacgttcc cctcggtgac
     1201 aagattgtca tcacaaaggt cgcttcctac atcgacaaca tgggctacaa taaagaggtg
     1261 gcctacctgc cgagcgactt caatgtctac agtgatttca ctgataccta cggatttgac
     1321 atgacctact ccgaaacgga ttacccgcaa ggcgaaggcg agttcgtcta cctgaactgg
     1381 gatgccgatc ccgtagtcgg aatcgcaggc tttgatgtca tgcttcacca gtatctttac
     1441 tactccgaag tcgaaacaag tccagttgaa actaccgatt ttgaagtgga tggacaacca
     1501 taccaactgg aaaaaaggtt ggatggcacg tattatatct tgacggtaaa ggatgcggca
     1561 ggagaagaat tgattgccta caatacccgg gaagtgtacg atactatttt cgagcagtct
     1621 gaaacgtcag gattcgataa ggggaatctg acaatcgagg atgcgacgat aaccactgaa
     1681 aatgaccaga tcagaatgaa tattttggtc aattccttgg aacgcacacc ggatttcaca
     1741 ggtggagatc tttatctctt tattgaattc aaataacaag aggtcagtaa gcatactctc
     1801 ctagccccgg atgcgcaaat gcagcgcatc cggggcttct ttattcatca gtggacttta
     1861 ttgtgcggaa atcatccacg gccggcaaac tctgtgatat gatggatgta ctattttcat
     1921 caatcaaggg aaggtgtggg gttgtgtcga aaggaaaagg gaaagtgatg ctgtcgatgc
     1981 tgatattcgg gacaatcagc atcttcgtta agaatatcga tttggcgtcc aatcaggttg
     2041 ccttcttcag gggagcgttg gggagtctgt tcctctgggg gacattgatg gtgcgcaagg
     2101 aaaagatccc tttggcgttg gtcaggaaaa atgccaagtt cctgttgctt tcgggttttg
     2161 ctgtcggcat gaactggatt ttgctgtttg aagctttccg gtacacgacg gtatcgaatg
     2221 cgacgttgag ctattatttc cagccgattt tcatgacgct gttggcgccg ttcgttttca
     2281 aggaatcctt gaccgcgaaa aagatcgcgt gcgtcctttt ggcgatgctg gggatgttcc
     2341 tgattatcgg cgtgagcccg aatgcgggga attataatca tccggtcggg atcggcttcg
     2401 gtttggctgc agcatttttc tatgcggtgg ccattatgtc caacaaaaaa attgtaggtc
     2461 ttggcgggat cccgctgact gctttaacgc tcgtcatcgc aacaggaatc ctgactcctt
     2521 atgtcgctct gacttccggt ttcggattag gacaattgac tggcggctcc atcaccagtc
     2581 tgctggtttt gggaatcatc cacacgggaa ttgcctatgt gcttatgttt gcaggcgccc
     2641 aagttgtcga gagccagaca ttcgctgtcc tcagttatgt cgatccggtg acggcgattg
     2701 tggtttcggc cgtattcctg aaagaagact tatcacccgt gcaattggcg ggaggcgtgc
     2761 tgatactggc tgcggccttc ctgaacgaat ttctgggcaa ccgggacaaa cagatagccc
     2821 tcagcaaaga atagcgaaaa agaaaaacgg aaacgaggca gcctcgtttc cgttttttgc
     2881 cgaagaacca caaaaacccg gacagatctg tccgggtttc gcagagaagg acgcctcatt
     2941 gcgtatggca gtacgcagat aaggcctttc ttctcaatga tggagtcatt tttttccgac
     3001 gcttactgac tgagcgatct cacgtcggga ggatttcatt aagaaatgaa aggccctctt
     3061 ttatggatgc actcagtata agccataact atttgcgaaa aaagatgccg agccccgaat
     3121 gaaagaaatt tctcagaact tgcatccata cggtttgaac actgagagga ttctttcaga
     3181 agaccttgtt gacttagtga acctcaccag gtgattcgac tgattttttt gtaataatcc
     3241 attttcattg cgacattgtg atcgtagtcg agtttgaaat agctggtgta gaggcaatcc
     3301 agcaaaaaca gtaatgaaag cctggtcgaa aacgaagaga tcttattgta atggctttca
     3361 tttgaactca tgtacaggtg gtgcgtggca gcacccgcga aagggatcgc atcggtggat
     3421 gtgatcaaca gaatgggtga tcggttattc ttcaagattt tcacgatttc gggaatgacc
     3481 gtcccccgtc cgccgtatga aacaacaatc gacaggtgcg agggatcact ggctgccgct
     3541 gtcagacgct gatgatattc ttcgatcggg acattgacga atctgccgat ttcctgcatc
     3601 tgaaacttga agttctcggc gaaaaacaga ttgccggcag aggtatacag gtcgatggat
     3661 ggtacctttt tcatcaagga agccgcctgc cgcagctgct ccagatcgag attgttgagc
     3721 gaggacacaa ccgtctgttc gtagagttcc tgcatcgtcc gcgtaatctg atattgggtt
     3781 tcgttggggt ggatcggata gttgaaatcc agcgttttcc gttctttcag atagtctccg
     3841 accgaggccg aaaccagaat cttcaattcg gaaagtccgg ccaaatccaa ctttttgcat
     3901 aagcggtaaa tcgttgcgtt cgaaataaaa ctggcttcgc tgatttccgc tgaagtcatt
     3961 ttcacaaaag cctcgggatt ttcttttata taggaaacca aaattttctc attttcggtc
     4021 aggctcacta aattgtccat tttgctgaag atattcatct gtcttcacct cattccttca
     4081 gaaagttgcg caggaaatcc gattccgaaa gcatcgtcaa attgtggccg ctgccttgca
     4141 gctcgcggac tgcctggatt ttgcggctct cgctcggtgt gccgatcttg cgccaatcgc
     4201 tgtcggcgac gaccaaataa agggtctcgt aacttagatt ggcatcgaaa tggccgccga
     4261 cgtcgacgac gagctgggcc aaatcgttgc gcttcagttt ttggaagcga ccggtaaagc
     4321 agacgtgctt gtcgtaaaag acgtgttccc tatcgaacag caggctcttc ggcttcagcg
     4381 gtgccgccgt ttttgttcgt ctggcaggcg tacttttgcc gcctttcgcc actccgaaag
     4441 catgcgagaa caatttgccc atccggtatt ggtattcgtc cagaaaacct tggatatcat
     4501 agtcgtattg cgccagcatt ttgctggtga tcagcccgca ggcgaccgca tcctccacgg
     4561 cattatggtg attatcgatc gtcatgccga agtagtccaa cacggtcggc aagcggttat
     4621 tgtgcagatc cagcatacgc tgcgccatct tgcaggaaca gaaaaactca ttctgcatct
     4681 tcggcagttt gtaggtttca atcgtttgtt gcagacaact catgtcgaat tgggcaaagt
     4741 gggccaccag cggatcggtt ccgatgaagg aacggatgtc tgggtatagg ccggcaaagt
     4801 cgggttcacg agcgaccatg tcctcggtga tgccgtggat ttgaatattc ccgcggctga
     4861 aatattggcg cggattgatg agcgcatagt agcggtcgac ttccttaccg tcggaaaagc
     4921 ggctcaagcc gatggaacag acgctgtcac cacggctgtt tgcagtttca aagtcaatgg
     4981 cgacgtaatc catgtgcata catactcctt tttggttgtt ttttgaataa tgaatagatg
     5041 gcctattgct gctgcaaggc cataatctgt tggtaataag ggatcatgct gcccaaagcc
     5101 gcgccgatga tgaagaacaa tagcatgcgg atcagcaggt aagcccagct ttccgagtaa
     5161 tcgtgctccg gatttttgtt cgagcggcgg aagaggatga acatgccgat ccccgcaatc
     5221 cagccgatgc ccgacaaaag cgcctgcgaa gtcgcaggat tcaggtagcc gatcacgaag
     5281 ccgacaaccg tggcaaagac caacaaaagc cgctgcttca ttttttctgt catcaaaatt
     5341 tccccctttt tcgtaaactg tccagtgatt caagcctgcc cctcggaaat tagataaatc
     5401 gtacctattg cgctctacga tgctcagtcg gtacgatttc cgatttccta aatttctttc
     5461 gaggcagatc gggcttgtca cacttttcta gcctattcta ccacaaagcg cgcttccgga
     5521 atacggttgt gatccggcaa aaagtgagcg gattgtatgt ctgtcgggtg aaccgctcgc
     5581 ttcgcgacgc aaacaaccga gagacagcgc atctgtcggg tgaacggctc gctgcgcgat
     5641 gcaaacaacc gagagacagc gcatctgtcg ggtgaacggc tcgctgcgca tcgcaaacaa
     5701 ccgagagaca gcgcatctgt cgggtgaacc gctcgcttcg
//
LOCUS       DAOMUJ010000058         5708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3839_length_5708_cov_2.617194, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000058 DAOMUJ010000000
VERSION     DAOMUJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_5708_cov_2.617194"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1152)
                     /locus_tag="PLT73_02505"
     CDS             complement(172..1152)
                     /locus_tag="PLT73_02505"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRM19285.1"
                     /translation="MTEHYKDPKLKIFSLSSNRPLAEKIAADVGVELGTLSSSQFSDG
                     EIKINIDESIRGCHVYVIQSTSSPVNDHLMELLIMIDALKRASAKTINIVLPYYGYAR
                     QDRKAQSREPITAKLVANMITNAGADRLLTLDMHAPQIQGFFDIPVDHLLGAPLLASY
                     FLDHEVLHDDVVVVSPDHGGVTRARKLAEFIKAPIAIIDKRRPKANVAEVMNIIGNVE
                     GKKCILIDDMIDTAGTITLAAQALQDAGALDVYACCTHAVLSGPAMDRINNSVIKELI
                     VTDSINIPAEKVSDKIVMVSVSQLIAEAIKRIHENRSVSPLFQEKFVIID"
     gene            complement(1249..2625)
                     /gene="glmU"
                     /locus_tag="PLT73_02510"
     CDS             complement(1249..2625)
                     /gene="glmU"
                     /locus_tag="PLT73_02510"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRM19286.1"
                     /translation="MANRFAIILAAGQGTRMKSKLYKVLHAVCGKPMVEHVVDQVEAA
                     GIEKVVTIVGHGADTVKGCLGDRSEYVLQAQQLGTGHAVLQAQAVLENEEGTTLVICG
                     DTPLLTAETLESLLQYHEEKKAKATILTAHAADPNGYGRIIRNASDNVEKIVEQKDAS
                     PEEVLVQEINTGTYAFDNKFLFAALQSVGNNNAQGEYYLPDVIEILKNQGEIVTAYQM
                     SDMGEALGVNDRVALAEASVYMKRRINEKHMRNGVTLIDPDNTYIESGVVIGSDTVIE
                     PNVFLKGKTIIGEDCFIGSGSELSNTTIGDRVQVRSSNLEDSVMESDSNIGPFSHLRP
                     NSHIGKRVHIGNFVEVKNAILNEDTKVGHLTYVGDADLGKNINVGCGTVFVNYDGKNK
                     HRSSIGDNTFIGCNVNIVAPVTVEANTLLAAGSTITKDVPEGAMGIARSRQENKPGYW
                     EKLPPAKK"
     gene            2832..3365
                     /locus_tag="PLT73_02515"
     CDS             2832..3365
                     /locus_tag="PLT73_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM19287.1"
                     /translation="MTKPTASFWIHELLLQPHAEGGYFRETGHSPELIVTDDRRERYL
                     YSNILFLLTADNPSHFHRLKADEVWYFHEGSALTIHLLHPDGRYEAVSMGRHPEKGEL
                     FQFTVPKGVIFGSSVEGSSGDYALVSCMVSPGFDYNDFELLTYDALIKTYPDYGSILE
                     KLAYKKIPETSFRKDHV"
     gene            complement(3472..4299)
                     /gene="purR"
                     /locus_tag="PLT73_02520"
     CDS             complement(3472..4299)
                     /gene="purR"
                     /locus_tag="PLT73_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HRM19288.1"
                     /translation="MKIKRSERLVDMTRYLLERPHRLISLTYFAKRYESAKSSISEDL
                     SIIKKTFEDRGTGIVETLPGAAGGVKYIPTINKENAREIIEEMCEIMNSKDRLLPGGY
                     VYLSDMLGNPEILQKIGRVIAAQYVDKKVDAVMTVATKGVPIAQAVATYLNVPFVIVR
                     RDSKITEGSTVSINYVSGSSDRVEKMELSKRSLPRGSKVLIVDDFLKGGGTINGMRSM
                     ISEFESETVGVTVFAEAGYNSKREVFDYTSLIRVNNIDLKDHSISVGLGNYFDPSTD"
     gene            complement(4458..5267)
                     /locus_tag="PLT73_02525"
     CDS             complement(4458..5267)
                     /locus_tag="PLT73_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRM19289.1"
                     /translation="MEMFLYGFMQRAFQASTLIAIMAPVLGLLLILRRQSLMADTLSH
                     VSLAGVALGMLLNINPTLTTLGIVILAAIGIEYLRVIFKGYSEISVAILMATGMAVAL
                     VLMSFVSGKQNASVEQFLFGSIITINDDQILILLALAIIVVLLYLIFRKPLYVLTFDE
                     DTAHTAGLPVRMMSLSVSVITGVAISVVMPITGSLLVSSIMILPAAISMRLVHTFNGV
                     ILLGIGIGLTGMYGGLFASYQYGTPPGATITLVFVAMFILTSIYKVLVEKK"
     gene            complement(5213..>5708)
                     /locus_tag="PLT73_02530"
     CDS             complement(5213..>5708)
                     /locus_tag="PLT73_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRM19290.1"
                     /translation="LTIGYAPQQISSFNIGFPSSVLEFVESGRYQKGRWFKKLDEEDH
                     THVKKALESVGMWEMRNKKIGELSGGQKQRIALARIFATDPDLFVLDEPTTGMDKESR
                     ERFYRLLKHNSQVHNKAIIMVTHEHEDIKEFVDTHIQLVRKEDSPWRCFSMDLCKEPS
                     KHQH"
BASE COUNT         1467 a         1379 c         1186 g         1676 t
ORIGIN      
        1 ccctgatttg ggctcgaaaa catggattct tttgagttat tcatcttttc cggcaccact
       61 atccacacct gcctacgcct gctctctcca cataactcct aaccaacgga caaccgcacc
      121 ttcaaacaaa aaagccctcc caagggagga cttttttcaa caatcgcatt cttagtcgat
      181 gattacaaat ttttcttgga acaatgggct tactgagcgg ttttcgtgga tacgtttgat
      241 tgcttcagcg attaattgtg acacagaaac cattacgatc ttatcgctga ctttttcagc
      301 agggatattg atcgaatcag taacaatcaa ttctttgatt acggagttgt tgatgcggtc
      361 catggctgga cctgacaata cagcatgtgt gcagcaagca tagacatcca acgcgcctgc
      421 atcctgcagt gcttgggctg ccaatgtgat tgtacctgca gtatcgatca tgtcgtcaat
      481 caggatacat tttttgcctt cgacattacc gatgatattc atgacttcag caacgttcgc
      541 tttcggcctt ctcttgtcga tgatggcgat tggagctttg atgaattcag ccagtttgcg
      601 ggctctggtc acgccaccat gatcaggcga aacaacgact acatcatcat gcaaaacttc
      661 atggtccaag aagtagcttg ccaatagcgg agctcccaac aaatgatcga caggaatatc
      721 gaagaaacct tggatttgag gagcatgcat atctagggtc aacaatctgt cagcgccagc
      781 attggtgatc atgttcgcca ctaatttcgc tgtgatcggc tcacgtgatt gcgctttgcg
      841 atcttggcgt gcatagccat aataaggcaa cacgatattg atcgttttgg cactcgcgcg
      901 cttcaaagca tcgatcatga tcaaaagttc catcaggtgg tcgtttaccg gagaacttgt
      961 cgattggatg acgtaaacgt ggcaaccgcg gatactttca tcgatgttga tcttaatttc
     1021 accgtcgctg aattgagatg aagaaagggt tcctaactcg acaccaacat cagccgcaat
     1081 tttctcagcc aaagggcgat ttgaacttaa ggaaaaaatc ttaagttttg gatctttgta
     1141 atgttctgtc atgataatcc tccgttgatc ttttgttttt accaaattta acacatgttg
     1201 atattataat ttcaaaatta ttctaccata catacataag gttttgtttt attttttggc
     1261 tggcggcaat ttttcccagt agcctggctt gttctcctgg cggctgcggg caatgcccat
     1321 cgcgccttca ggcacatctt tcgtgatggt cgagcctgca gcaagtaatg tattggcttc
     1381 aacagtgact ggcgccacaa tattcacgtt gcagccgatg aatgtattgt caccgatgct
     1441 ggaacggtgt ttgttttttc cgtcatagtt cacgaagaca gtgccgcaac cgacgttgat
     1501 atttttaccg agatcagcat ctcccacata agtcaaatgt ccaacttttg tgtcttcgtt
     1561 caaaatcgca ttcttcactt ccacaaaatt tccgatgtgg acgcgttttc caatgtggct
     1621 gttcggtcgc aagtggctga atggaccgat gttgctgtcc gattccatca cggagtcttc
     1681 caagtttgaa cttctgactt gtacgcggtc accgattgtt gtgttgctca gttctgagcc
     1741 cgatccgatg aagcaatctt ccccgatgat cgttttccct ttcaggaata cattcggttc
     1801 aataactgtg tcgcttccga tgaccacacc cgactcgatg taggtattat ccggatcgat
     1861 caaggtaacg ccgttgcgca tatgtttttc attgatgcgt ctcttcatgt agacggaggc
     1921 ttcagccaac gccacacgat cgttcactcc caaagcttcg cccatgtcgc tcatctggta
     1981 agctgtcacg atttcacctt ggttcttcag gatttcgatg acatccggca agtaatactc
     2041 gccttgcgca ttgttgttgc ctactgattg cagcgcggca aacaaaaact tgttatcgaa
     2101 cgcatatgtg ccggtattga tttcttgcac gagcacttct tccggagaag catctttttg
     2161 ttccacaatt ttttctacat tatctgacgc atttctgatg atgcggccat agccgttcgg
     2221 atcagcagca tgcgcagtca ggatagttgc ttttgctttt ttctcttcat ggtattgcaa
     2281 caaggattcc aaagtttcag ccgtcaggag cggtgtatct ccgcagatga ccaacgtagt
     2341 gccttcttcg ttctccagca cagcttgcgc ttgcaggaca gcgtgccctg ttcccaattg
     2401 ttgcgcctgc aatacatatt ccgaacgatc ccccaagcat cctttaacag tatccgcacc
     2461 gtgccctaca attgtcacta ccttttcaat gccagcagcc tctacttgat ccacgacgtg
     2521 ctctaccatt ggttttccgc aaactgcatg cagtactttg taaagcttac ttttcatgcg
     2581 cgttccttga ccagcagcaa gtatgattgc aaaacgatta gccataagca actcctatct
     2641 tttcatttta ttccttcaac taaattatct tagcgtaaaa tcttgctttt ttcaatatga
     2701 tgccgtatat aaatacagac ttcttttcat tttctaatgg aaatacgaag aatgcgtata
     2761 cattattact tattgaccgg ttttttgttt ataatgatga acagaaagat acattgaaag
     2821 gattggttcc aatgacaaaa ccgactgcat cattctggat acacgagttg ttgcttcaac
     2881 cgcacgcgga aggtggctat tttcgcgaaa ccggccattc tcctgaactg atcgtcaccg
     2941 acgaccggag agaacgctac ctatacagta atatactttt tttgctgact gcagataacc
     3001 catcccattt tcaccgtttg aaggccgatg aggtctggta tttccatgag ggcagcgcct
     3061 tgacgatcca cctgttgcat cccgatggcc gttatgaagc ggtcagtatg gggcgccatc
     3121 cagaaaaagg tgaactcttc caattcacag ttccgaaagg ggtcatcttc ggaagcagtg
     3181 tagaaggctc gtccggagac tatgctcttg tgagctgtat ggtttcccca ggattcgatt
     3241 ataatgattt tgaattattg acttatgatg cattaatcaa aacctatcct gattacggga
     3301 gcattctgga aaaattagcc tacaaaaaaa ttccggagac ttctttccgg aaagatcatg
     3361 tgtgatttct tccgaaattt aactcggtga atagcgctgt tttgtcaatc cgcaacaaag
     3421 agccgaccct tgatgcgttc aaggagccag ctcttttttt catagttatt ttcaatcggt
     3481 gcttggatcg aaataattgc ccaagcccac actgatggaa tggtctttca aatcaatatt
     3541 gtttacgcgg ataagcgatg tataatcgaa tacttcacgt ttggaattgt agcccgcttc
     3601 tgcgaatacg gttacgccga ctgtttccga ctcaaattca gaaatcatgc tgcgcatacc
     3661 attgatggtg cctccacctt tgaggaaatc atcgacgatc agaactttgg aaccgcgggg
     3721 cagactgcgt ttggacagtt ccattttttc tactcgatct gatgaacctg agacataatt
     3781 gatgctcaca gttgagcctt ctgtaatttt cgaatcacgg cgtacgatga cgaacggcac
     3841 atttagatac gtagcgactg cttgcgcaat cggaacccct ttggtggcga cagtcatgac
     3901 agcatccact tttttgtcaa catattgagc cgcgatgaca cgtccgattt tctggagaat
     3961 ttccggattg cccaacatat cagaaagata aacgtatcct cccggtaaca gacgatcttt
     4021 actgttcatg atttcgcaca tttcttctat aatttcgcgg gcattttctt tgttgattgt
     4081 cggaatatac ttgacgcctc ctgctgcacc cggcagcgtt tcaacaatcc ccgttccacg
     4141 gtcttcaaat gtttttttga tgatggatag atcttcactg atggaagatt tcgctgattc
     4201 atatcgttta gcgaaatagg tcaatgaaat caaccgatgc ggtctttcta gtaagtaacg
     4261 ggtcatatca actaaccgtt cactgcgctt tattttcact ccaaatctct cctcatattg
     4321 cgttctttcc tctattatat agttaattgc tgaaaaacaa aagcttttaa ttgtttatcg
     4381 ttcgttttta atcgaatttt agcaaaaaaa acaagagcaa tgttctgctc ttgtccatat
     4441 atcttattgt taagattcta cttcttttcg actaggacct tgtagatact ggtcaaaatg
     4501 aacatggcca cgaaaaccag ggtgattgtg gctcccggag gcgtcccgta ctgataggat
     4561 gcgaacaatc ccccgtacat tcccgtcaaa ccgatgccta tcccaagcag gatgaccccg
     4621 ttgaaggtat gcactaacct catcgagatg gctgcaggca gaatcatgat cgaagaaacc
     4681 aacaatgatc cggtaatcgg catcaccacg ctgatggcaa cccctgttat tacagacacc
     4741 gacagagaca tcatgcggac cggcaagccc gctgtatggg ccgtgtcttc atcaaacgtc
     4801 aatacgtaca atggcttgcg gaaaatcaga tacaataaaa cgactatgat tgccaaagcc
     4861 aacaagataa gaatctggtc atcatttatc gtgatgatcg atccgaacag gaactgttcg
     4921 acacttgcgt tctgtttccc tgacacgaag ctcatcaaca ccaaagcgac tgccatcccc
     4981 gtagccatca gaatcgcaac cgatatttcc gaataccctt tgaagatgac gcgcaaatat
     5041 tcgattccga ttgccgccaa aataacgatg cctaaagtgg tgagcgtcgg attgatgttc
     5101 agaagcattc ctaaagcgac tcccgctaag gatacatggg ataatgtgtc cgccatcagg
     5161 gactgccgtc gtaaaatcag cagcaagccc aaaacaggcg ccatgattgc aattaatgtt
     5221 gatgcttgga aggctctttg cataaatcca tagagaaaca tctccaaggc gaatcctcct
     5281 ttctgaccag ttggatatga gtatccacaa attctttgat atcttcgtgc tcatgcgtaa
     5341 ccatgatgat cgctttgttg tgtacctgcg aattgtgctt cagcaaacgg tagaaacgct
     5401 cacgcgattc tttgtccatt ccagttgtcg gctcatcgag cacaaacaaa tccggatcgg
     5461 ttgcgaaaat acgtgccaag gcaatgcgtt gtttttgccc accggaaagc tccccgattt
     5521 ttttgttgcg catctcccac atacccacgg attccaaagc tttttttaca tgggtatgat
     5581 cttcttcgtc caattttttg aaccatctac ctttttggta tctgccggat tccacaaact
     5641 ccaagacact gcttggaaag ccgatattga acgacgaaat ttgctgcggg gcataaccaa
     5701 tcgtcagc
//
LOCUS       DAOMUJ010000059         5691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3857_length_5691_cov_2.555358, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000059 DAOMUJ010000000
VERSION     DAOMUJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_5691_cov_2.555358"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLT73_02535"
     CDS             complement(<1..622)
                     /locus_tag="PLT73_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM19291.1"
                     /translation="MRYKPNYAPGPTEVRENVRLARAKRTNNSDFDKTFVRYYREVSQ
                     KMGKVMGTTNEVLLLAGEGILALEAACASLTEPGDRVLVLDNGIYGEGFKDFVSIYGG
                     VPVVLSFDHHSGIPAEAVRAYLEKDSDFKYATLVHCDTPTSVLNNVAELCPLLKEYGI
                     LTVVDSVAGMVGEPINVDANRIDICCGGTQKAISAPVGLAIVSVSAD"
     gene            927..1640
                     /locus_tag="PLT73_02540"
     CDS             927..1640
                     /locus_tag="PLT73_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRM19292.1"
                     /translation="MQGISEIQKDLSSYTLKNLAIFAMFFDHIFAVFVPQDSLEGVAL
                     RILGRIAAPIMCYMIAEGYHYTSNLKKYIVRLALFAAVSHFPYVWYFGLPWWQATSVI
                     WGLALGLVALAAAKREDWSHPLKVFIVLICCLLAVPADWNYVAVLWILFFGLFRGQIE
                     KQLLSFAIIGILFHIIPSIKEIGWTQSYQVGIFLAVPLLLFYKGRQGKKSNLMKWGFY
                     VFYPFHLLLLELVKMIVSA"
     gene            1660..2838
                     /locus_tag="PLT73_02545"
     CDS             1660..2838
                     /locus_tag="PLT73_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRM19293.1"
                     /translation="MLIRQIWRRVFIGILTGMVLSACSGSNEQTETNESKTASSSSTA
                     EAGTSEEAAVVRPEESEPLLYEGDLELPVNGATGYASVLMDLKATADAGSETISELEA
                     GTAFEVLEEVGDWWYVRTATESGWVQHLYCFINLPDVVPSIIYDNTNTYASKFASSGK
                     TIPGITGEALYDGKAYNMRLGKVSDIVPVLYSMSKKIHLAQQAALEEGNTLVIYEGYR
                     PFFAQKLTVDALTTLAAADPEVMAGINTHPWDTNWFIATSISNHQMGYAIDVTLAKIT
                     EQQEFVIGDYTATAVTGYTEYTMPTTIHELSMASATFTGPVKSSSPTAWLQANLADTM
                     NEAAILLQRYCTDAGLTPLASEWWHFNDLDARFATEDNSSKGEYLLDATMSEAPEAAE
                     "
     gene            complement(2927..2998)
                     /locus_tag="PLT73_02550"
     tRNA            complement(2927..2998)
                     /locus_tag="PLT73_02550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2964..2966),aa:Val,seq:gac)
     gene            3162..3686
                     /locus_tag="PLT73_02555"
     CDS             3162..3686
                     /locus_tag="PLT73_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HRM19294.1"
                     /translation="MDNNDILIRLRYALDIKDADMVEVFRLGGLEVTKETVQQMLKNP
                     KKDEATDAAGAAAPSGDDQLACDNATLESFLNGLITSQRGKPQVKPGEPEPKPEFLLN
                     NGNVNNLLLKKVKIALTLTNEDMLEILESAGVTVSKSELSAVLRKEGHRNYKPCGDRY
                     ARNFLKGLALRYRG"
     gene            complement(4037..4996)
                     /locus_tag="PLT73_02560"
     CDS             complement(4037..4996)
                     /locus_tag="PLT73_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM19295.1"
                     /translation="MKKTNQKNTNNNKRNRQVKATEPQKSPSTKGVTQYPVTEPSELL
                     PFLLQILSKQSRNSVKSILTRGQVTVDGKTITKHNHPLEPGQTVGILANKEALKKTSL
                     SGLKILFEDEAIIVINKDAGLLSMATDDPKEPTAYRQLSQYVKEDNKANRIFVVHRLD
                     RDTSGVMLFAKSEELKEKLQNNWKEIVKERIYTALVEGEIKKDAGTISSWLTESKAMK
                     VHSSPKDNGGKHAVTHYRKIRANKEYSLLEVQLETGRKNQIRVHMESLGHPVAGDKKY
                     GARTNPMKRLGLHATTLAFIHPATGELVRFSAPVPKAFLLYSK"
     gene            5160..>5691
                     /locus_tag="PLT73_02565"
     CDS             5160..>5691
                     /locus_tag="PLT73_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19296.1"
                     /translation="MEYISISGAKEGNLKNISVDLPRNKWIVLTGVSGSGKTTLAMDV
                     LYQECQRQYLEAIGYQGIRKPDIDAIRNVSPAIQIQQQAYNKNPRSTVGTVTDIYTDL
                     RMLYEKLSTRDCPACGKEFSSADCREETEKTADDFKVFQYCPHCAQRMEKLTPTHFSY
                     NTREGACATCQGLGEVM"
BASE COUNT         1387 a         1359 c         1509 g         1436 t
ORIGIN      
        1 cgtccgcact gacggaaacg atggccagtc cgactggggc tgaaatggct ttttgggttc
       61 cgccgcagca gatgtcgatc cggtttgcgt cgacgttgat cggctcgccg accatgcctg
      121 ctacggagtc gaccaccgtc aatatgccgt attcctttag caacggacaa agctcggcta
      181 cgttgttcag taccgatgtc ggtgtgtcgc aatggaccag ggtggcatat ttgaagtcgg
      241 aatccttctc cagataggcg cgtactgctt cagcggggat gccgctgtgg tgatcgaaac
      301 tcaggacgac cggtacgccg ccataaatgc tgacgaaatc cttgaagcct tcgccataga
      361 tgccgttgtc cagcacgagt acgcggtcgc caggttccgt caggcttgcg caagcggcct
      421 caagagccag gatcccttca ccagcaagta acaggacctc atttgtggtg cccatgactt
      481 tgcccatttt ttggctgacc tcacggtaat agcggacgaa tgttttgtca aagtcgctgt
      541 tgttggttct ttttgcgcgg gccaaacgta cattttcgcg tacctcagtc ggacccgggg
      601 cgtaattcgg tttgtatctc atcaggaagt cctccttctc gtttcgtata ttgctgataa
      661 ttttagcaaa tgcaaaggac tatgggaccg tccaatccga gagaaagtga acggtccaat
      721 ttgcccaaaa atggcttctc gcatgcgttt tgcaaatgtt aacgtatcca atttcggatt
      781 gcgtaagttt ttccgcaaat ctcttccttt ttttcgggga ttggcggact tcaggaagct
      841 tatactgtgg caaagggcag gcaaactggc ctgtcgatca gcttacagca aacgctgcga
      901 aaccaagtac agtgaggagc acaccgatgc aaggcatcag cgagattcaa aaagatctat
      961 catcctatac attgaagaac cttgcgatat ttgcgatgtt cttcgatcat atttttgcgg
     1021 tatttgtgcc gcaggacagc cttgaaggtg ttgcactgcg catattaggg cgtattgccg
     1081 ccccgatcat gtgctatatg attgcggaag gctatcatta cacatccaat ctgaagaaat
     1141 atatcgtgcg gttagcgctg ttcgccgccg tttcgcattt tccgtatgtc tggtatttcg
     1201 gtctcccgtg gtggcaggca acgagtgtca tctggggttt ggcgttggga ttggtggcat
     1261 tggcggcggc caaacgggag gactggtccc atccgctgaa agtcttcatc gttttaatct
     1321 gctgtctatt ggcggtcccg gctgattgga attatgtagc ggtcttgtgg atcctgtttt
     1381 tcggattgtt caggggacaa atcgaaaagc agctgctgag ttttgcaatc atagggattc
     1441 tgttccacat catcccttca atcaaagaaa tcggttggac gcaatcctac caagtcggca
     1501 tttttctggc ggtaccgcta ctgctgttct acaaaggccg tcaaggcaag aaatccaacc
     1561 tgatgaaatg gggcttctat gttttttatc cgttccatct gttgctgttg gagctcgtga
     1621 aaatgattgt atccgcttaa tggaggactg aatatacata tgttaataag acaaatttgg
     1681 cgccgcgttt tcatcggcat cctgacaggg atggtgctgt ccgcttgctc cggctcgaat
     1741 gaacaaacgg aaacgaatga gtctaagacc gcttcgtcta gttcgactgc cgaagcggga
     1801 acttccgaag aagcagcggt ggtccggccg gaagagtcag agccattgct gtatgaaggt
     1861 gatctggaac ttccggtcaa cggagcgact ggttacgctt cggtactgat ggatctgaag
     1921 gcgactgcgg atgccggatc cgaaacgatt tcggaattgg aggcgggtac cgcttttgag
     1981 gtgctggagg aggttggcga ttggtggtat gtccggaccg ctactgaatc aggatgggtg
     2041 cagcatcttt attgttttat caatcttccg gatgtggtcc cctccatcat ctatgacaat
     2101 accaacacgt acgcatccaa gttcgcttct tccggaaaaa cgatcccagg cataaccggg
     2161 gaggcgcttt acgacgggaa agcatacaat atgcggctgg gaaaagtgtc cgatatcgtg
     2221 ccggtgcttt acagtatgtc gaagaaaatc catttggcgc agcaagcagc tttggaagag
     2281 gggaatactt tggtcatcta tgaggggtac cgtccatttt ttgcccaaaa gctgacagtg
     2341 gatgctttga caaccttggc tgcggccgat ccggaagtga tggcgggcat caatacccat
     2401 ccttgggata ccaactggtt tatcgcgaca agcatttcga atcaccaaat gggctatgcc
     2461 atcgatgtca ctttggcgaa aattacggaa cagcaggaat tcgtcatcgg cgactatacc
     2521 gcgaccgccg taacgggcta tacggaatat accatgccga caaccatcca tgaattgagc
     2581 atggcttcgg ctacgttcac cggaccggtc aaatccagct cgccgacagc gtggctccaa
     2641 gccaacttgg ctgatacgat gaatgaggcg gcgattctcc tgcagcgcta ttgcaccgat
     2701 gccggactga cccctttggc ttcggaatgg tggcatttca atgatctgga cgcgcgcttc
     2761 gctaccgaag acaacagcag caaaggcgag tacttattgg atgcaacgat gagcgaggct
     2821 ccggaagcgg ccgaatgagg aataaacaag gctcaaaatg gacataaaaa gaggcacaat
     2881 cgaatttctc gatcgtgcct ctttcctatt ttgtactgaa gcttgatggt ccctactggg
     2941 attgaaccag cgacccctac catgtcaagg tagtgctctc ccgctgagct aaggaaccgt
     3001 taaacagatg aatcacctgc tcgtatatct gattataaca tatttgcgcg agaagtgaag
     3061 aggtaaatta aaaaaatgtg gagcgaataa ggcatgcatt ccacatgaca acaaggtata
     3121 ctggaaaagg ataattttac aggaaaattt ggaggtttaa tatggacaac aatgatatat
     3181 tgatccgctt gcgctatgcg ctggatatca aggatgcgga tatggtggaa gtgttccgat
     3241 tgggtggact cgaagtgacg aaggaaaccg tacagcagat gctcaagaat ccaaagaaag
     3301 atgaagcaac tgacgctgcg ggcgcagctg caccgtcagg cgatgatcaa ctggcttgcg
     3361 ataatgcgac actggaatct ttcctgaacg gactcatcac ttcccaacgc ggcaaaccgc
     3421 aagtgaagcc cggcgaaccg gaaccaaagc cggaatttct gctgaacaac ggaaacgtca
     3481 acaatctgct gctgaagaaa gtcaaaatcg cgctgacttt gacaaacgaa gacatgctgg
     3541 aaattctgga atcggcaggc gtcaccgttt cgaaaagcga actgagcgcg gtcttgcgca
     3601 aagaagggca ccgcaactac aagccttgcg gcgatcgtta tgcgcgtaat ttcctaaaag
     3661 ggctggcgtt gcgttaccgc ggataggctg gaattgaatt tatgttgaaa taaaccgcca
     3721 aaatggtttt ggcggtttat tttagttcaa accacagtct gaaaacccgc aaaagccact
     3781 ttggcgggga atgccactat gaacgctacg aactcgaacc ccattccaga caaacatacc
     3841 cccaaaccac aaagaagagg ctgggacaaa actccagcct caaattatat attcgaatat
     3901 tatttgcgta aacgtgcgcc aaaaggcagg ccctggctcc acttcacgga gaatgatcag
     3961 atggtctgcg accatcctgc gcttcttcgc tccagtgatc cgggcttaaa cgcctattga
     4021 cccactctct ttcttcttat ttcgaataca gtagaaaggc tttcggcacc ggtgcgctga
     4081 agcggacgag ctcaccggtc gcaggatgga tgaaagcgag tgtggtagcg tgcagcccca
     4141 aacgcttcat cggattggta cgggcaccgt atttcttatc tccggcaacc ggatggccca
     4201 aagattccat gtggacgcgg atctgattct tgcggccggt ttccagttga acttccagga
     4261 gcgagtattc cttgtttgcg cggatcttcc ggtaatgggt cacggcatgc ttgccgccgt
     4321 tgtctttcgg actggaatga accttcatgg ctttgctttc ggtcagccac gaggaaatcg
     4381 tgcctgcatc cttcttgatt tcgccctcga ccagagcagt atagatgcgt tctttgacga
     4441 tttctttcca attattttgc agcttttctt tcagctcttc ggattttgcg aacagcatga
     4501 cgccggaggt atcccggtcc aggcgatgca cgacgaatat ccggtttgct ttgttgtctt
     4561 ctttgacgta ttggcttaat tggcggtaag cggttggttc tttaggatcg tcggttgcca
     4621 tcgataacag gccggcatcc ttattgatca caatgatggc ttcatcctca aacaggatct
     4681 tgaggcctga cagggacgtt tttttgaggg cttccttgtt cgccaaaatg ccgacagtct
     4741 gcccgggttc caagggatgg ttgtgcttgg tgatggtctt cccgtctaca gtcacttgtc
     4801 cgcgggtaag tattgatttg actgagttgc ggctttgctt gctgaggatc tgcagcaaga
     4861 agggcaacaa ttcgctcggt tcggtcacag ggtattgggt gacccctttg gtggacgggc
     4921 ttttttgggg ttctgtcgcc ttgacctgac gattgcgttt attgttattt gtattctttt
     4981 ggtttgtttt tttcatctga tttcgctcct gactctgttc gccgcctgag tatcgcggtc
     5041 gtctctcttc tgaagtataa cagaaaactg agcatccatc ccgcctaatc aggacaaaaa
     5101 tgcagcaagt ggcatataat tgaaacaaaa ggatatcaaa aggaagcgag ggagcagtca
     5161 tggaatacat aagcatcagc ggtgccaagg aaggcaatct gaagaacatc agcgtggact
     5221 tgccgcggaa caaatggatc gtcctcacag gggtatccgg ttccgggaaa acgactttgg
     5281 cgatggatgt gctttatcag gagtgccaac gtcaatatct ggaagcaatc ggctaccaag
     5341 gtatacgcaa gccggatatc gatgcgatcc gaaatgtgtc ccccgctatc caaatccagc
     5401 agcaagccta caataaaaat ccgcgttcga ctgtgggaac ggtcacggac atctataccg
     5461 atctgcggat gctgtacgag aagttgagca caagggactg cccggcttgc gggaaggaat
     5521 tcagttcggc ggattgccgt gaggaaaccg aaaagaccgc tgatgacttc aaggtgttcc
     5581 agtattgtcc gcattgcgct cagcggatgg aaaagctgac ccccacccat ttttcctaca
     5641 acacccgaga aggcgcctgc gctacctgcc agggattggg agaggtaatg a
//
LOCUS       DAOMUJ010000060         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3863_length_5688_cov_2.242855, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000060 DAOMUJ010000000
VERSION     DAOMUJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3863_length_5688_cov_2.242855"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..974
                     /locus_tag="PLT73_02570"
     CDS             447..974
                     /locus_tag="PLT73_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HRM19297.1"
                     /translation="MLNKFKPTWMVESIYHITPEQIEKNGIKGVLTDLDNTLIAWNNP
                     EGTQELKDWIALMKKNAIPVVIISNNSDKRIKIIADKLQLAYVPRSMKPSRRGYIKAA
                     EQLQLPLDQCLMVGDQLLTDVFGANRAGVKSVWVMPIINSDGWNTRINRFFERKLLKR
                     LLKNDPGMIWRKSLD"
     gene            970..>1805
                     /gene="yqeH"
                     /locus_tag="PLT73_02575"
     CDS             970..>1805
                     /gene="yqeH"
                     /locus_tag="PLT73_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HRM19298.1"
                     /translation="MKEELTEELYCIGCGAKIQTDDPTERGYTPAAALQKGLDSGQVY
                     CQRCFRLRHYNQMEKVSVTDDEFLAMLNTISMEDALIVNVIDLFDVYGSMIPGLHRFA
                     GNNKVLVVGNKVDVLPKSVKLSRVKQWLTEQVQSVGLRPVDVMLVSGKKATSIDDLLE
                     TIEEHRDGKDVYVVGVTNVGKSTVMNQIIRAATGNKEDVITTSQFPGTTLDQIRIPLD
                     DGHFLIDTPGIIHHHQMAHVLSPKELKLVAPQHEIKPITYQLNPEQTLFLGGASRFDF
                     ISG"
     assembly_gap    1806..1815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1816..2100
                     /locus_tag="PLT73_02580"
     CDS             <1816..2100
                     /locus_tag="PLT73_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="in Bacillus subtilis this enzyme appears to be
                     involved in 30S ribosomal RNA subunit biogenesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HRM19299.1"
                     /translation="KSSFTCYFANDLKIHRTKLEKADEFYAKHLGTLLQPPSAEDAEN
                     FPPLVRREFNVKVPSDIVFSGLGWITVRKPGKVAAWVPQGVDTVMRKPII"
     gene            2122..2433
                     /gene="yhbY"
                     /locus_tag="PLT73_02585"
     CDS             2122..2433
                     /gene="yhbY"
                     /locus_tag="PLT73_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRM19300.1"
                     /translation="MELRGKQKQYLKKEAHHMNPLFQVGKGGLNEEMLYQIGEALEKR
                     ELLKIALLQNTDEETDAVAAVIEEKLRATVVQQIGRTLVLFKPSSKEKNRRYSTVVEK
                     I"
     gene            2511..3137
                     /locus_tag="PLT73_02590"
     CDS             2511..3137
                     /locus_tag="PLT73_02590"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRM19301.1"
                     /translation="MIIEETEVETQIAFQDRKRIGIMGGTFSPPHLGHLIVAQQVGEQ
                     LGLDKIYFMPDAEPPHIDEKESISAKHRERMVRLSISGNPLFDIETIELERGGKSYTI
                     DTMKLLTAMHPDTDYYFIIGGDMVEYLPKWKDIDALVQLVQFVGVARPGYGRESVYPI
                     IWVDAPLIDISSTEIRKMVKTGRSIRYLVKEDVKDYILEEGLYLEDED"
     gene            3121..3741
                     /gene="yqeK"
                     /locus_tag="PLT73_02595"
     CDS             3121..3741
                     /gene="yqeK"
                     /locus_tag="PLT73_02595"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRM19302.1"
                     /translation="MRTKTELVYTGRYTDWTREAILAEVEKQMKPSRFQHVLRVEDCS
                     IALAKKYGASLEACSLAALLHDFAKEHDAKSMKKIVLSQALDSEMTGYGSEIMHGPVG
                     AYYAEKVFGIMDRAILDAIRQHTIGGEVMTLIGKVLFIADYIEPGRTFTGVDEARRLA
                     EISLDEAAYFKIEKTIIHLVKKELPIYPGTIHVYNSWIQRKVGNSE"
     gene            3738..4103
                     /gene="rsfS"
                     /locus_tag="PLT73_02600"
     CDS             3738..4103
                     /gene="rsfS"
                     /locus_tag="PLT73_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRM19303.1"
                     /translation="MKMTSEELLEVVVKAADDKLAQDIMALDVRGLTSIGDYFIVMNG
                     RNEKQVAAIVESIVENVHKNKGEIKNVEGKDSGKWTLIDLNDVIVHVFNHEERGYYNI
                     EKLWSDAPMVDISGMVSEQ"
     gene            4100..4843
                     /locus_tag="PLT73_02605"
     CDS             4100..4843
                     /locus_tag="PLT73_02605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM19304.1"
                     /translation="MSYDIFAHYYDEIMDQSVYDSWLLYVEKYTAGKSGLDIMELACG
                     TGKIAVDLAKKGHQVTGVDLSDEMLSLAYNRMQEEGVTLSLAVGDMRSLEPMGDFDVV
                     TCFSDSLCYMSDEAEVAEVFQSVSRNLGPGGVFLFDVHSMHQVNDVFPGYQYIYQDEA
                     GAFLWESFAGDAANSVEHDLTFFVGSQSNPELFERHSEYHRERTYPLEVYKRLLEEAG
                     FTDVTATAEFGEADVRDDSPRWFFACKKA"
     gene            4901..>5688
                     /locus_tag="PLT73_02610"
     CDS             4901..>5688
                     /locus_tag="PLT73_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM19305.1"
                     /translation="MKACGVIAEYNPFHNGHRYQLSEARRMTKSDVMVVAMSGNFVQR
                     GAPALLDKWTRAEIALWNGADIVVEMPILGSVQSADLFARAGIRLLQAMQCDAFAFGA
                     EEGTAEDFISHSRFLREHEAEINRIFQTYRNDGRTYAAQLETAIAEILSPQGSAISFS
                     TPNNQLGLAYVKENEQFPEPMETAIVLRRGAGHNDSEAFGEEFASGTAIREWALHHGK
                     QSEGNDMGRFVPEETLEALERLPLLDWAPYWGMLQYQLMLLSHA"
BASE COUNT         1559 a         1207 c         1518 g         1394 t
ORIGIN      
        1 tgcgatttca atcgcataga gacatgatac acttgggcgc ttgcgaccac agtgctaccc
       61 gatactctcc cgcatagcat cgcgccttct taaacgcgct ctgattcgca gacaccactc
      121 ccgtttcgca agtggctgtc gtccacaaaa gaggtgaacg tcagccgctt gctccaacgt
      181 ccggatgtct aggcgcgaat catcgcgctc ttgtattttt tggtttatgc tttataatgt
      241 tattggaatg gggttataat atacaattgg atagtagtga ggctgggtca aaactttttt
      301 gcggggtaca gggtgcttgt ttcggattgt gtgccgtgca gttattcggt tttggggcgc
      361 gacttggggg agttctctca ggttctgctt gaaaagtgac gttgttgctt tttcccatcc
      421 tcttattttt ttaatgagga gacattatgc taaataaatt caaaccgaca tggatggttg
      481 agtccatcta tcacattact ccggaacaaa ttgaaaagaa tggcatcaaa ggcgttctga
      541 ccgatttgga caataccttg attgcctgga acaatccgga ggggacgcag gaattgaaag
      601 attggatcgc cttgatgaaa aaaaatgcga tacctgtcgt catcatttcc aacaacagcg
      661 ataagcgtat caagataatc gcggataagc tgcaattggc ttatgtgcca aggtcgatga
      721 agccttcccg ccgtgggtac atcaaggcgg ctgagcagct tcagcttccg ttggatcaat
      781 gcctgatggt tggggatcaa ttgcttacgg atgtttttgg cgcaaatcga gcgggagtca
      841 agagtgtgtg ggtaatgccg atcatcaatt cggacgggtg gaatacgcgc ataaatcgat
      901 tttttgaaag aaaattgctg aaacgtttgt tgaaaaatga tccaggaatg atatggagga
      961 aatcacttga ttaaagaaga attaacagaa gaactctatt gcatcggctg cggggctaaa
     1021 atccagaccg atgatccgac cgaacgaggg tataccccag cggccgctct gcaaaaggga
     1081 ttggattccg ggcaagtata ctgccaacgc tgtttcagat tgcgccatta caatcagatg
     1141 gaaaaagttt ccgtcacgga tgatgaattt ttggcgatgc tgaacaccat ctcgatggaa
     1201 gatgctttga tcgtcaacgt tatcgatctg ttcgatgtat acggcagcat gatccctggc
     1261 ttgcatcgct ttgcgggcaa taataaagta ttggttgtcg gaaacaaagt ggatgttttg
     1321 ccgaagtcgg tcaaactatc ccgtgtgaag caatggctga cggagcaggt gcagtcagta
     1381 ggcttgcgcc cggttgatgt catgcttgtg agcggcaaga aagcaacttc catcgatgat
     1441 ctgttggaaa ccatcgaaga acaccgggac ggcaaagatg tttatgtcgt gggtgttacg
     1501 aacgtgggta aatccactgt catgaaccaa atcatccgtg cggctacagg caacaaggaa
     1561 gatgtcatca cgacttccca gttcccgggc acaacgttgg atcagatccg tattcctttg
     1621 gatgatgggc atttcctcat cgatacaccg gggattatcc atcaccatca aatggcgcat
     1681 gttctgagcc cgaaagaatt gaaacttgta gctccgcaac acgaaatcaa accgatcact
     1741 tatcaattga acccggagca gacgcttttc ttgggtggcg catcgcgttt tgatttcatc
     1801 agtggnnnnn nnnnnaaatc atccttcact tgctatttcg caaacgactt gaaaattcac
     1861 cgcaccaaat tggaaaaagc ggatgaattc tatgcaaaac atctgggtac gctgctgcaa
     1921 ccgccatctg cggaagatgc ggaaaatttc ccgccattgg tcagacgaga attcaatgtc
     1981 aaagtaccgt ccgatatcgt attttccgga ttgggatgga tcactgtccg caaaccgggt
     2041 aaagtcgcag catgggtccc gcaaggcgtg gacacagtaa tgcgcaaacc gatcatttaa
     2101 taaaaaagac tgaggtgtcg aatggaatta agaggcaaac aaaaacaata tttgaaaaaa
     2161 gaagcacacc atatgaatcc gctcttccaa gtgggtaagg gtgggttgaa cgaagagatg
     2221 ctttaccaaa tcggggaagc actggagaaa agggaattgc tgaaaatcgc tcttctgcag
     2281 aacacggatg aggaaacgga tgctgttgct gcagtcatcg aagaaaaact gcgagcgacc
     2341 gtcgtccaac agatcggacg cacacttgta ttgttcaaac catcatctaa agaaaaaaat
     2401 cgtcgttatt cgactgtcgt tgaaaaaatc taaaggcagg ccaattccta ttttattgag
     2461 gtgagaaaaa attgatcaca actttaaatt tgatcggaca aacggattac atgatcatcg
     2521 aagaaaccga ggttgagaca cagattgctt tccaggaccg gaaacgcatc ggcatcatgg
     2581 gtggaacctt cagtccaccc catttgggcc atctgatcgt tgcgcagcag gttggggaac
     2641 agctcggttt ggataaaatc tatttcatgc ccgatgccga accgcctcat atcgatgaaa
     2701 aagaatcgat ttcggcgaag catcgtgagc ggatggtgcg attgtccatt tcaggcaatc
     2761 ctttgttcga tatcgaaacg atcgagttgg agcgcggcgg caaaagctat acgatcgata
     2821 cgatgaaatt attgacggcc atgcatccgg acaccgatta ttatttcatc atcggcgggg
     2881 atatggttga atacttgccg aagtggaaag acatcgatgc attggtgcaa cttgtgcagt
     2941 tcgttggcgt tgctcggcca ggatatggaa gggaaagcgt ttatccgatc atctgggtcg
     3001 acgcaccgtt gatcgacatc agttcgacgg aaatccgtaa gatggtcaaa acgggccgtt
     3061 cgatccggta tttggttaaa gaggatgtca aagattacat tttggaagaa ggactctatc
     3121 ttgaggacga agactgagct tgtatacaca ggacgctata cggattggac acgggaagcc
     3181 attttggcgg aagtggaaaa acagatgaag ccatcgcgct tccagcatgt actgcgggtt
     3241 gaagactgtt ccatcgcgtt ggcaaaaaaa tatggtgcat ctttggaagc atgctcgttg
     3301 gccgctctcc tgcacgactt tgcaaaagag catgacgcaa aaagtatgaa gaaaatcgtc
     3361 ctgtcccaag cgttggacag cgagatgacc ggttacggca gcgaaatcat gcatgggcct
     3421 gtcggtgctt attatgctga aaaggtattc ggaatcatgg atagggctat ccttgacgcg
     3481 attcgtcagc atacaatcgg tggtgaggtg atgaccctga tcggaaaagt cctattcatt
     3541 gccgattaca tcgaaccggg ccgtacgttc acgggcgtgg atgaagccag aagactggcg
     3601 gaaatcagcc tggacgaggc agcttatttt aaaatagaaa aaacaatcat tcatctggtg
     3661 aagaaggaac tgccgattta tcccggcacg atccatgtct acaacagttg gatccaaaga
     3721 aaagtgggga attcagaatg aaaatgacaa gtgaagaatt attggaagta gtggttaaag
     3781 ctgcagatga caaattggct caggatatca tggcattgga tgtacgcggt ttgacatcaa
     3841 tcggtgatta ttttatcgtt atgaacggcc gtaatgagaa gcaagtggct gcaatcgttg
     3901 aatctattgt ggagaacgtg cacaaaaaca aaggcgaaat caaaaacgtc gaagggaaag
     3961 attctggcaa gtggacattg atcgacctga atgacgttat cgtgcatgta ttcaaccatg
     4021 aggaacgcgg ttattacaac atcgaaaaat tatggagtga tgcgccaatg gtcgacatct
     4081 cggggatggt ctctgagcaa tgagctatga catctttgcc cattactatg atgaaataat
     4141 ggaccagtct gtctacgaca gctggcttct ttatgttgaa aaatacacag ccgggaaatc
     4201 tggcctggac atcatggaac tggcttgcgg aactggtaaa attgcagtgg atttggcgaa
     4261 aaaagggcat caggtaaccg gggtcgatct atccgatgaa atgctctcgc ttgcctacaa
     4321 tcgcatgcaa gaagaaggcg taacgctttc cttggcggta ggggatatgc gttccctgga
     4381 gccgatgggg gattttgacg tcgtgacgtg cttctctgat tctctttgct acatgtctga
     4441 tgaagctgaa gtggcggaag tctttcaaag cgtgagccgg aatctcggac ctggcggtgt
     4501 ttttttgttc gacgtccact cgatgcatca agtgaatgac gtttttccgg gatatcaata
     4561 catctatcag gatgaagcgg gagccttcct ctgggaaagt tttgccggcg atgctgcgaa
     4621 cagtgtcgag catgatttga ctttcttcgt gggatcccag agtaatccgg aattgtttga
     4681 acgtcattcg gaataccatc gcgaacgcac gtatccgctg gaagtctaca agcggctcct
     4741 ggaagaagcc ggatttaccg atgtgacggc cacagccgaa tttggtgaag ctgatgtgcg
     4801 cgatgacagt ccgcgctggt tttttgcctg caaaaaagct taaacaagcc aaggcaaaaa
     4861 aaccgcgcga ccatacaatg aaaccaaaag gggcgcttcg atgaaagctt gtggtgtaat
     4921 tgcagaatac aatcctttcc ataacggtca ccgctaccaa ctgtcggaag ccaggcggat
     4981 gacgaagagc gatgtcatgg ttgtcgcaat gagcggcaat tttgtccaaa ggggcgcgcc
     5041 ggctttgttg gataaatgga cgcgtgcgga aatcgctttg tggaacggtg ccgacatcgt
     5101 cgtggaaatg ccgattttgg ggagtgtgca gtccgcggat ctgttcgcaa gagcaggcat
     5161 ccgtttattg caggcgatgc agtgcgatgc ctttgcgttc ggtgctgaag aagggacggc
     5221 ggaagatttc atttcgcaca gccgtttctt gcgcgaacac gaagcggaaa tcaatcggat
     5281 ttttcagacg taccgaaatg atggcagaac ctatgctgcg caactggaga cggccattgc
     5341 cgagatcctg agcccacaag gttctgctat ttccttttca acgcccaata atcagctcgg
     5401 attggcgtat gtcaaagaaa atgaacaatt ccccgaaccg atggaaacgg ccatcgtgct
     5461 taggcgcggt gccggacata acgacagtga agctttcggc gaagaatttg caagtgggac
     5521 cgccattcgc gaatgggcgc tccaccacgg aaagcaaagc gaagggaatg acatgggcag
     5581 gtttgtgccg gaagagacgt tagaggcgct ggagaggctt cctctgcttg attgggctcc
     5641 atactggggg atgctgcaat atcagctcat gctgctttcg catgcgga
//
LOCUS       DAOMUJ010000061         5611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_3954_length_5611_cov_2.313355, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000061 DAOMUJ010000000
VERSION     DAOMUJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3954_length_5611_cov_2.313355"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLT73_02615"
     CDS             <1..732
                     /locus_tag="PLT73_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM19306.1"
                     /translation="PSNSQQYSKISAHSEESQDSSSAIVAESESKAIVTPPASSADWN
                     LVLVNRENQLAEEIPMEFYLTESGYQIDSRIQEPYSQLMEAGKAAGMDLTMVSGYRSI
                     EQQQTNYDVNYQNYLASGLSEEEARTKTEEYIALPNASEHITGLAVDITSTALANQEG
                     NSGLFPDLENYPEGLWLKENAPKFGFVLRYPKEKEAITGINFEPWHFRYVGIENAMYM
                     TEKNLTLEEYIAILKQNEALGNNNQ"
     gene            770..1390
                     /locus_tag="PLT73_02620"
     CDS             770..1390
                     /locus_tag="PLT73_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HRM19307.1"
                     /translation="MVRKRKRTRKQPQSAPAKAVKILFFLLISFMASLSLIGDFSPIV
                     PEIPQYGNNQGSDFIADIAPAAQQMQANYGVHASISIAQAILESDWGESELSAVYNNL
                     YGMKGDNPENTVRLMTSEYYNGEWVTIQANFRVYGSWAESVQDHALLFVNGTTWDPDQ
                     YAPVLQAATYQEAAQALQDCGYATDPDYAEKLIAVIEQHALYEYDI"
     gene            1606..2736
                     /gene="purK"
                     /locus_tag="PLT73_02625"
     CDS             1606..2736
                     /gene="purK"
                     /locus_tag="PLT73_02625"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRM19308.1"
                     /translation="MPNQILPGKTIGIIGGGHVARMIALEAKKMGYQIGILDADPACP
                     AGQIADWQITKKYTDFDALTDFAAKSDVLTFESEAVDTMLLTQINPYIAIPQDPDSLS
                     ITQDRLIEKAFLESLNINVTPYATITAIEDMEEAVKSIGFPCVLKPLRIEMATPVQHL
                     LYSEEDFERAAALLKKGTCILEAWIPFEMELAITVAQDANNVFTSFPVTETIYREQKL
                     VKTITPARVGGNIQKELEHIGKLAAKAMNLTGILTIETFMTSSGTLYVNRLVVRPHHS
                     CNYSLDGCSISQYEVLVRCICGLPIPEIQLYDTSVTFNIMEEKLDEALILLLTKPDWH
                     FHFYGKKEHRAKRKMGHVTLLGDSPDTLINELEENGLLESAE"
     gene            2780..3436
                     /locus_tag="PLT73_02630"
     CDS             2780..3436
                     /locus_tag="PLT73_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM19309.1"
                     /translation="MEDRLFNKAYDYIKDKIDSNSWPPGTKITEQLISEALFMSRTPI
                     RTALLLLEEEGLVKSIPYKGYIVAEKKISNEGLLERLELIAGLIMNYLQYLKENDIAL
                     DYQLFETILEKQTEYLDYRDATGYFKNEYRIFETFIAPSENQFLKKVIMTTARDIHST
                     YSDNTDLMDEERYQSEAQLLELYKEIAVCMRDKDYEKMGEYLLFFFETLKDSNDSLAQ
                     "
     gene            3727..>5611
                     /gene="pcrA"
                     /locus_tag="PLT73_02635"
     CDS             3727..>5611
                     /gene="pcrA"
                     /locus_tag="PLT73_02635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HRM19310.1"
                     /translation="MQQKHDLIKGMNPRQAEAVLATEGPLLIMAGAGSGKTRVLTHRM
                     AYLLEEKSVNPWNILAITFTNKAAKEMKERVIRLVGTVGNDMWVSTFHSMCVRILRRE
                     SEQLGYARSFTICDQSETETLMKRIIREKNLDAKKFDHRMILGRISQAKNELQTPKEF
                     GDLAGGYIDNIVYECYKDYQKALENSQSMDFDDLIMLTVKLFQEYPETLSYYQNKFHY
                     IHVDEYQDTNYAQYQLVQLLANKFKNICVVGDADQSIYGWRGADMENILNFEKDYPDA
                     KVVLLEQNYRSTKKILRAANDVIQNNVNRKEKELWTDNPDGENIVYYRGQSEHDEARY
                     VISKMQQEVRENKRQYGDFAVLYRTNAQSRVIEENLLKSNIPYKMVGGRKFYDRKEIK
                     DILAYLRLIVNPSDDLSFGRIVNVPKRGIGDASVEKLREFANMHDLTLLQAAHDVAMS
                     SVSGKAAKELKKFATIMDNLHKMQEFLPITELVQELLSQTTYLEALEMEKTLESEARI
                     ENINEFLSVTQEFDKMNPEDFDLTSFLTDLALVSDLDQIEEEPQSEVTLMTMHAAKGL
                     EFPIVFIIGMEDGIFPSSRSFMDEDQMEEERRLAYVGITRAEQRLFLTNAYSRMLYGR
                     TMSN"
BASE COUNT         1688 a         1327 c         1384 g         1212 t
ORIGIN      
        1 ccgtcaaaca gtcagcagta ctcgaaaata agcgcccatt ccgaagaatc acaagactct
       61 tcctcagcca tagttgctga atcggagtcc aaagcgatcg tgacaccacc agcttcatca
      121 gctgattgga atctggtatt ggtgaaccga gaaaatcaac ttgctgaaga aatcccaatg
      181 gagttttacc tgaccgaaag tggctatcag atcgacagca gaatccagga accttattcg
      241 caactgatgg aagccggcaa agccgccgga atggatctca cgatggtttc aggttaccga
      301 tccatcgagc aacagcaaac aaactatgac gtcaattatc agaattatct agcttcgggc
      361 ttgtcggaag aagaagcacg cacgaaaaca gaggaatata tcgccttgcc gaatgccagt
      421 gaacatataa ccggacttgc agtagacatc acgtcgaccg ccttggccaa tcaagaaggc
      481 aattcaggat tatttccgga tctggagaat tatcctgagg gactgtggct gaaagaaaat
      541 gcacccaaat ttggttttgt gttgcgttat ccaaaggaaa aagaagccat aaccggcatc
      601 aattttgaac cttggcactt tcgctatgta ggcatcgaaa acgccatgta catgaccgaa
      661 aaaaatctga cattggaaga atacatagcg atcctgaagc aaaacgaagc actgggcaac
      721 aacaatcaat agcagctaaa gcaacccaaa caaacagacg gaggaacata tggtacgaaa
      781 gaggaagcgg actcgaaaac aaccgcaatc tgcacccgct aaagccgtca agatattatt
      841 ttttctgctg atcagtttta tggccagcct gtcgctgatc ggcgattttt ccccaatcgt
      901 tcccgaaatc ccgcagtacg gcaataatca ggggtccgac ttcatcgctg atatcgcccc
      961 tgccgcacag cagatgcaag cgaattacgg tgtccacgcc agcatcagca tcgctcaggc
     1021 gatccttgaa tcggattggg gagagagcga actgtccgct gtctacaaca atctgtacgg
     1081 catgaaggga gataacccgg aaaacacggt ccgcttgatg accagtgagt actacaacgg
     1141 cgagtgggtc accatccaag cgaacttcag ggtatacggc agctgggcgg aatcggtcca
     1201 ggaccacgcg ctgctgttcg tcaacggtac cacttgggat ccagatcaat acgctcctgt
     1261 tctgcaggca gccacttacc aggaagcggc ccaggctctc caggattgcg gttatgcgac
     1321 tgatccggat tatgccgaaa aattgatagc ggtcatcgaa cagcatgcgc tttatgaata
     1381 tgatatttga aggatgaaaa cagaaacgcg cggacaggct ggactctttt gaagaggcag
     1441 cctttatccg tgcgtttttt gcgccatgtg cccgaaattt ctacgaaaat tcgacaagag
     1501 ggcagttttt gcatggcgac attcgtcgaa tatagtgata aaatggtttg gtaaagactt
     1561 ttttaacaaa ttctaatgaa taatccaaga gagtagtgtg atcacatgcc gaatcaaatt
     1621 ttacccggga aaacaatcgg tattattggc ggaggacatg tcgcccgtat gattgctttg
     1681 gaagccaaaa aaatgggcta ccaaataggc atcttggacg ccgatcctgc ctgtccggct
     1741 gggcaaatag ctgattggca gataaccaag aaatatacgg acttcgatgc gctgacggat
     1801 tttgcggcaa aatcggacgt ccttaccttt gaatctgaag ccgtggatac gatgttgttg
     1861 acgcaaatca atccgtacat cgccataccg caggatccgg acagcctttc gatcacccaa
     1921 gaccggttga tcgaaaaagc gtttctggaa tccttgaaca tcaacgtgac gccttacgcg
     1981 acgataaccg ccatcgaaga tatggaagaa gcggtcaaga gtatcggctt tccgtgtgtg
     2041 ctgaagccgc tccgaattga aatggccaca ccggtacagc atcttttgta cagtgaggag
     2101 gactttgaaa gggcggcagc cttgctgaaa aaagggacgt gcattttgga ggcatggatc
     2161 ccattcgaga tggaactggc catcacggtc gcacaggacg cgaacaatgt gttcacgtca
     2221 ttccctgtca cggaaacaat ctaccgggag caaaaactgg tgaagaccat cacgcctgca
     2281 cgcgtaggcg gaaatattca gaaagagctg gagcatatcg gcaagctggc tgcgaaggcc
     2341 atgaatctga ccggaatttt gacgatcgag acattcatga cttcatcagg aacgctgtat
     2401 gtgaaccgtt tggtcgtccg cccgcaccat tcgtgcaatt attcgctgga tggctgcagc
     2461 atctctcaat atgaagtcct ggtccgctgc atctgcggcc tgcccattcc ggaaatccag
     2521 ctgtacgata ctagcgtgac gttcaatatc atggaagaaa aacttgatga agcgctgatt
     2581 ctcttgctga ccaaaccgga ttggcatttc catttttatg gaaaaaaaga acaccgcgcg
     2641 aaacggaaaa tgggacacgt caccttattg ggtgacagcc cagataccct catcaacgaa
     2701 ctggaagaaa acggcttgtt agaatctgca gagtaacggg aacggtgtcc gcgctttgat
     2761 atgaaggaag ggtatagcga tggaggatag actattcaat aaagcatatg actatattaa
     2821 agacaaaatc gacagcaaca gctggccgcc gggaaccaaa ataactgaac aactcatatc
     2881 ggaagccttg ttcatgagcc ggactccgat tcgtaccgcg ctgctgttgt tggaggaaga
     2941 aggcctggtg aaatcgattc cctacaaagg ctatatcgta gctgagaaga aaatttcaaa
     3001 cgagggtttg ctggaacgcc tggaactgat tgctggcctg atcatgaact acctgcagta
     3061 tctgaaagaa aacgacatcg ctttggatta tcagcttttt gaaacaatcc tcgaaaaaca
     3121 aacggagtac ctcgactatc gggatgcgac cggctacttc aagaatgagt acaggatttt
     3181 tgaaacgttc atcgctccta gcgaaaatca gttcctgaag aaagtgatca tgacgacggc
     3241 ccgtgacatc catagcactt attccgacaa cactgatctg atggatgaag agcgttacca
     3301 aagcgaagcg cagttactcg aattatacaa ggaaatagct gtttgtatgc gcgacaagga
     3361 ttacgagaag atgggggaat acctcctctt cttcttcgaa accttgaagg actccaatga
     3421 tagcttggcg cagtagactg actaaaaaga aagtcctgcc gctgaatcaa ctaaacgatt
     3481 cagcggcagg acctttttgt tggacggcat aatagggaaa atgaacctga accgcatcaa
     3541 ggggaacaag gggtaaaaac tatttaaaat atccggggat tcattgatag ccacgctcta
     3601 aaatgataga ctaaaccatg atgcaaatca gaagcgatga ggctgcacca tggccttgaa
     3661 cctaacaaaa atgaacgtac ccatatactc tccgaaacaa aggcaacaaa agaaaggatg
     3721 gcaaatgtgc aacaaaaaca cgacctcatc aaaggaatga atccaaggca agcagaagcc
     3781 gttttggcga cagaaggccc attattgatc atggcggggg caggcagcgg caaaacacgt
     3841 gtgctgaccc accgcatggc ttatttactg gaagaaaaat cggtaaaccc ttggaacatc
     3901 ttagccatca cctttacgaa caaagcggca aaagaaatga aagagcgggt tatccgcttg
     3961 gtcggaacgg tcgggaacga catgtgggta tccactttcc actccatgtg cgtacggatc
     4021 ctcagaagag agagcgaaca gctcggctat gcgcgctcct tcacgatctg cgaccagagc
     4081 gaaaccgaaa cattgatgaa acgcatcatc cgcgaaaaga atctggatgc caaaaaattc
     4141 gatcatcgta tgattttggg acggatcagc caagcgaaaa acgaactgca gacaccgaag
     4201 gaattcggcg atctggccgg cggatacatc gataatatcg tctacgaatg ctacaaagat
     4261 taccaaaaag cgctcgaaaa cagccaatca atggattttg acgacctgat catgctgacg
     4321 gtcaaactgt tccaggaata tccagaaacg ttgtcctact accaaaataa attccattac
     4381 atccatgtgg acgaatacca ggataccaac tacgcccaat accaattggt gcagctgttg
     4441 gccaacaaat tcaaaaacat ctgcgtcgtc ggcgatgcgg accaaagcat ctacggttgg
     4501 cgcggtgctg acatggagaa catcctcaac ttcgaaaagg attatccgga tgcaaaagtg
     4561 gtcttgctgg agcagaacta tcgctccacc aaaaaaatct tgcgtgcagc caatgatgtg
     4621 atccaaaaca acgtcaatcg gaaagaaaaa gagttgtgga ctgataatcc ggacggcgag
     4681 aatatcgttt actaccgggg ccaatccgaa catgacgaag cgcgctacgt catctcgaag
     4741 atgcaacagg aagtccgcga aaacaaacgc cagtacggcg atttcgcagt gctctaccgg
     4801 acgaacgccc agtcgcgtgt catcgaggag aatctcctga agtcgaatat cccttacaag
     4861 atggttggcg gacgcaagtt ctacgaccgc aaagagatca aagacatctt ggcttatctg
     4921 cgcttgatcg tgaatccgtc cgatgatttg agcttcggcc ggattgtgaa tgtgccgaaa
     4981 cgcggcatcg gtgacgcgag tgtcgaaaaa ctgcgtgaat tcgccaatat gcatgacctg
     5041 accttattgc aggctgccca tgatgtggca atgtccagcg tatccggaaa agctgcgaag
     5101 gaattgaaga aattcgcgac tattatggat aatctgcata aaatgcagga attcctgccg
     5161 atcacagaat tggtccagga gttgctttcc cagacgactt atctggaagc cctcgaaatg
     5221 gagaagacgc tggagtcgga agcccggatc gaaaacatca acgaattcct gtcggtaacc
     5281 caggagttcg ataaaatgaa tccagaagac tttgatctga cctccttcct gaccgatttg
     5341 gcgctggttt ccgatttgga tcagatcgag gaagaaccgc agagcgaagt gacgctgatg
     5401 acgatgcacg cagccaaagg actggaattc ccgatcgtct tcatcatcgg gatggaagac
     5461 ggcattttcc catcgtcccg ctccttcatg gatgaggacc agatggagga agaacggcgc
     5521 ctggcctatg tcggcatcac acgggcggag caaagactgt tcctgacgaa tgcctattcc
     5581 cgcatgcttt atggacgtac gatgtccaat c
//
LOCUS       DAOMUJ010000062         5564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4011_length_5564_cov_2.086223, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000062 DAOMUJ010000000
VERSION     DAOMUJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_5564_cov_2.086223"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>627)
                     /locus_tag="PLT73_02640"
     CDS             complement(<1..>627)
                     /locus_tag="PLT73_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19311.1"
                     /translation="VIVAVSTTAAIFIGTEDTLTVRLPYENQRTYYETVDLEEQLEAI
                     EKETETNGLNVTGTEMYVYYNLFSIIEDNKIMIGAADFAKEIPDTIQLLIREDYERIV
                     GETVPLSSDEVLLFDSKNTFGFSTVIIGNTTYQVKSKIDNPLMMDKSQSPNMIMIVDS
                     RETLESIMETDFRLREIDLPIVADAVISWNTTGTQAQKKDYSEKTAAVV"
     assembly_gap    628..637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<638..>857)
                     /locus_tag="PLT73_02645"
     CDS             complement(<638..>857)
                     /locus_tag="PLT73_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19312.1"
                     /translation="MSLTIDNPLDAMIYFFLAVLLVIAGTYFLFISGSIFILKKLKKN
                     KPFYYRPSPFISISGMLYRMKQNAVGLAN"
     assembly_gap    858..867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<868..1508)
                     /locus_tag="PLT73_02650"
     CDS             complement(<868..1508)
                     /locus_tag="PLT73_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM19313.1"
                     /translation="MNKFFFSKLALRNLKSNKQIYFPYIFASIATVAMFYMMVALRGN
                     KFIQTRSDTLSLLFALGAIVVGVFSFIFILYTNSFLIKRRKKEIGLYAILGMKKSHVS
                     KILTIESMVTSCFSIVVGVVVGHLLGELAFLVLNYALKFGIEMSFPFTIQAMLVTIGL
                     FALIFLTTLGYNITQVTFSNPIQLIKGKQTGEREPKSSLILFILSLLLIGSGY"
     gene            complement(1501..2268)
                     /locus_tag="PLT73_02655"
     CDS             complement(1501..2268)
                     /locus_tag="PLT73_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19314.1"
                     /translation="MTLLEVTHLKKTYTGIFSTQAVQALKDINFSVEKGEFVSIMGES
                     GSGKTSLLNILATLDTATAGEVMLEGKDLTQIKDSEISNFRRDYLGFVFQDFNLLDNF
                     SIKDNILLPLVLSNTPITEMENRLMPIIQKLKIDGLINHYPYEVSGGQKQRAAVARAL
                     ITNPKLLLADEPTGALDSKSSQSLLETFESINDAGQTIIMVTHSTRAAAYSNRVLFIQ
                     DGEVYHEIYRGEQTPDQFMDKISQALVVLANRGEQHE"
     gene            complement(2389..3417)
                     /locus_tag="PLT73_02660"
     CDS             complement(2389..3417)
                     /locus_tag="PLT73_02660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM19315.1"
                     /translation="MMKQNKKWADILIAFIKAERSLMILYMLNVMVFSLVYILGRLPL
                     YFLLFAIELSLFLGSCFFAFQFSRYYKRFRLLERLDKDPIHALKQLAASQDPSEAFLR
                     LKIVDLIKEIQQIERNNVQRSNSQMDYFTLWLHQIKTPISAISLLMQRDSQMNYRNQI
                     EQELLRIENYTHMALNYVRIEQSGTDLDFADVSLDRVIKETVKKFSILFIYNHIQLDY
                     QETAAIILSDEKWLRVLVEQILSNSLKYTPEGGTIKIYMSPTQEQQLIIEDTGIGIRS
                     EDLPRIFERGYSGFNGRIHEKSTGLGLFLSQEITKRLGHDLRIESTLGKGTKVTIDLA
                     REPLRRDH"
     gene            complement(3401..4096)
                     /locus_tag="PLT73_02665"
     CDS             complement(3401..4096)
                     /locus_tag="PLT73_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM19316.1"
                     /translation="MMKIMIIEDDLVIASSLQEELLKWQYSAFYVTDFNDVMNTFTQE
                     APQLVLIDIKLPYFNGYYWCTEIRKISQVPIIFVSSQSERMDIVMAIQMGADDFISKP
                     FDLTVALAKIQALLRRTYDFTGNDIYLSHRQVHLKTSELKVQYQDQEVELTKNEMKIL
                     EVLFLQKGAFVSREAIMMKLWENDSFIDDNTLAVNINRLRKKLAEINVTSFIVTKKGF
                     GYGLQKDEHDETK"
     gene            complement(4541..>5564)
                     /locus_tag="PLT73_02670"
     CDS             complement(4541..>5564)
                     /locus_tag="PLT73_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HRM19317.1"
                     /translation="FEIPKKYGLQQTIGDTTGIGGIFRGLRTLPVMFDIAKEMEEVCP
                     DAWLLNYTNPMAILTMGMLKATKIKTVGLCHSVQVCVPELFEHLGIKDQYNLDEFQWK
                     IAGINHMAWLLEINRNGEDFYPEIRRLASEIANPHKDSVRFELMKHFGYYITESSEHN
                     AEYHPYFIKKNYPELIDQLQIPIDEYLRRCVDQIAGWETQRDEIVNDGSLEHTRSREY
                     ASYIMDAITTGTPTMIAGNVLNKGLITNLPEDCCVEVPCLVDKNGVQPTYVGKLPTQL
                     AALNRTNINVQELTVEAAMTLEKDKIYQAALMDPHASSELSISEIKAMVDELIAAHGD
                     YLPAYK"
BASE COUNT         1584 a         1198 c         1026 g         1736 t
ORIGIN      
        1 acgacagcag ccgttttttc agaatagtcc ttcttctgcg cttgcgttcc agtcgtgttc
       61 caagaaataa ctgcatctgc aacaatcgga aggtcaatct ctcttaatct aaaatcagtt
      121 tccataatgg attctaaggt ctctctagaa tccactatca taatcatatt aggactctgt
      181 gatttatcca tcatcaaggg gttatcgatt tttgatttga cttgatacgt tgtatttccg
      241 attatcactg tggaaaaacc aaatgtattt tttgaatcaa acagtaatac ttcatcagaa
      301 cttaacggga cggtttcgcc aactatccgt tcataatctt ctctgataag cagctgaata
      361 gtatccggta tctctttagc aaaatcagcc gcaccaatca taattttgtt atcttcaatg
      421 atactgaaca gattatagta cacatacatt tctgtccccg taacgtttaa tccgttcgtt
      481 tctgtttcct tttcgatggc ttcaagttgt tcttccaaat cgacggtttc atagtaggtt
      541 cgttgattct cataaggtaa acgaaccgtc aaagtatctt cggtcccaat aaaaatagct
      601 gctgtcgttg ataccgcaac aatgaccnnn nnnnnnnatt ggctaaccct actgcatttt
      661 gtttcatccg ataaagcatc ccggagattg aaataaatgg actcgggcga taatagaacg
      721 gcttattttt cttcagtttt ttcaggatga aaatagagcc ggaaatgaat aagaaatacg
      781 tacccgcaat aaccaacagt actgcaagga aaaaataaat catggcatca agcgggttat
      841 caatggtcag cgacatcnnn nnnnnnncag tatcctgatc caatcaataa caatgaaaga
      901 ataaacagta taagcgaact tttcggctcc cgttctcctg tctgcttgcc tttaatcagt
      961 tggataggat tagaaaacgt aacttgagtg atattgtaac ccaacgttgt taaaaagatg
     1021 agggcaaaca gtccgatcgt aaccagcatt gcctgtatcg taaaaggaaa agacatttct
     1081 atcccgaact ttaaagcata gttcaaaaca agaaaagcaa gttctcccaa tagatgcccc
     1141 actaccacac ctacaacaat actaaaacag cttgttacca ttgattcaat cgtcaatatt
     1201 ttggaaacgt ggctcttctt cattcccaat atggcatata ggccaatttc tttttttcga
     1261 cgtttaatca ggaaactgtt ggtatacaaa ataaagataa aggaaaacac cccaacgact
     1321 atagccccta aagcgaaaag caaagacaac gtatccgatc tggtttgaat aaatttattt
     1381 ccccttaacg cgaccatcat gtaaaacatc gctaccgttg caattgaagc aaaaatataa
     1441 ggaaaataaa tttgcttatt ggatttaagg ttacgcaggg ccagctttga aaaaaaaaat
     1501 ttattcatgt tgctcacctc tgtttgccaa gacaaccaac gcttgcgaga ttttgtccat
     1561 aaactgatcc ggtgtctgtt cgccacggta aatttcatga tacacttcgc catcttgaat
     1621 aaacaataca cggttagagt aagctgcagc ccttgtactg tgggtaacca tgataatcgt
     1681 ctgccccgca tcatttattg attcgaaagt ctccaataga ctttggctgg atttcgaatc
     1741 caatgctcca gtgggctcat ctgcaagcaa taattttgga tttgtgatca aagctcgggc
     1801 tacggctgcg cgctgttttt gtcccccaga aacctcatac ggataatgat tgatcagccc
     1861 atcaattttc aatttttgaa tgatgggcat caaacgattc tccatttcag ttattggcgt
     1921 attagataag acaagcggca ataaaatatt gtcttttatc gaaaaattat ccaataagtt
     1981 gaaatcttgg aaaacaaacc ctaggtaatc tctacggaag ttagaaatct cactatcttt
     2041 gatctgtgtc aggtcttttc cttccagcat tacttcaccc gcagtagctg tatcaagtgt
     2101 tgccaaaata ttcaacaagc ttgtttttcc tgaacccgat tctcccataa tcgagacaaa
     2161 ttctcctttt tcaactgaaa aatttatgtc ttttaaggct tggactgcct gtgttgaaaa
     2221 tattcccgta taggtctttt ttaaatgcgt cacttctaat aatgtcatgt ttgttgtctc
     2281 ctgttcattt ctttgtccct atcactagtt taatgaaaat gaacatcggc tgcctttatc
     2341 ttttcttaca aagggttgct taaccttaca gttttgtaag attacccatc aatggtccct
     2401 ccgtaacggt tctctggcta aatcaatcgt gactttggtg ccttttccta aagttgattc
     2461 gatccttagg tcatgtccca aacgttttgt aatttcctga ctcagaaaca atcccagacc
     2521 cgttgacttc tcatgtatcc ttccattaaa tcctgaataa ccacgttcaa atatgcgcgg
     2581 taagtcttca gaacggatac caatacctgt atcttcaata atgagctgct gctcttgtgt
     2641 ggggctcatg taaatcttga tggtgccacc ttcgggcgta tatttcaaac tattggaaag
     2701 aatttgttca accagaacac gcagccattt ctcatctgac aagataattg ctgctgtctc
     2761 ttgatagtct aattggatat gattgtaaat aaacaaaata gaaaattttt ttaccgtctc
     2821 tttgattacg cgatcgagcg aaacatctgc aaaatccaaa tctgtaccac tttgttcaat
     2881 tctcacataa ttcaatgcca tatgtgtata attttcaatc cgtagcagct cttgttcgat
     2941 ttggttccga taattcattt gactatctcg ttgcatcaat aagctgatgg ctgaaatggg
     3001 tgttttgatc tgatgcagcc acaaggtaaa ataatccatc tgagaattgc ttctctgcac
     3061 attatttctt tcgatttgtt ggatctcttt gattaagtct acgattttaa gacgaagaaa
     3121 agcttcactt gggtcttggc tagcagctaa ttgtttcagc gcatggattg gatctttatc
     3181 caggcgttcc agtaatcgaa accgcttgta atacctcgaa aattgaaatg cgaaaaaaca
     3241 actgcctaaa aacaaagaaa gctcgatagc gaatagcaaa aaatagagtg gcaatcgtcc
     3301 caatatatag acgagactga acaccatgac gttaagcata tataggatca tcaaagagcg
     3361 ttcggcttta ataaaagcta tcaaaatgtc tgcccatttc ttattttgtt tcatcatgct
     3421 cgtccttctg aagtccatat ccaaatcctt ttttcgtgac aataaaggaa gttacattta
     3481 tttctgcaag ttttttgcgt aagcgattga tgttgacagc taatgtattg tcatcaataa
     3541 aggaatcatt ttcccatagt ttcatcatga tggcctctcg ggaaacaaag gccccttttt
     3601 gcagaaagag tacttctaaa attttcattt catttttggt taattctact tcctgatcct
     3661 ggtattgaac ctttaattca cttgtcttca gatgtacctg ccgatgactc aaataaatgt
     3721 cattgccggt aaagtcatag gttctccgca acaatgcctg tatttttgct aaagcaacag
     3781 ttaaatcaaa gggcttactg ataaaatcat ctgctcccat ttgtatcgcc attacaatat
     3841 ccattcgttc gctctgtgaa gaaacaaaaa taatgggtac ttgagagatt ttacgaattt
     3901 ccgtacacca ataatacccg ttaaagtacg gaagtttgat gtctattaag accaattggg
     3961 gtgcctcttg cgtaaatgtg ttcattacgt cattgaaatc agtgacataa aaggcactgt
     4021 attgccactt cagcaactct tcctgcaaac ttgaggcaat gactaaatca tcttcaataa
     4081 tcataatctt catcaaccta ttcacctact catccttcat tcgttgcttc tactatacag
     4141 gattaattcc cgttagccta ttggaccttg gacttagtaa ccattttgac tgttcaatta
     4201 tttcacaaga taagcatccc actaacaaaa tatggctgta taatttttag tgtggatgag
     4261 tacagagaaa aagctgtctc gttgacgagg cagctttggt tgggcgttat tctttttggg
     4321 gggtgtccga taattgcgaa gaatcggaca tttaggtagc ttattgtgac gcggatgtcc
     4381 gataacttca tagaatcgga catccaggat gactataccg gcatgcttgt ccgataactc
     4441 tatagaatcg gacatctacg cagactacgc tgtcctgctt gtccgataat cgcaaaatat
     4501 cggacaacct gaagcaggag agcgattcgc aggcactgtt ttatttataa gccggcagat
     4561 aatcgccatg ggcagcgatc aattcatcca ccattgcttt tatttcggaa atggacagct
     4621 cggaactagc atgcgggtcc atcaacgccg cttgatagat cttgtctttt tcaagtgtca
     4681 ttgcggcctc aaccgtcaac tcctggacat tgatgtttgt ccggttcaat gcagccaatt
     4741 gcgtagggag tttgcctaca taggtaggtt gaacgccatt tttgtccacc aaacacggaa
     4801 cttccacaca gcaatcttcc ggtaagttgg tgatcaggcc tttgttcagg acattcccgg
     4861 caatcatcgt tggcgttcca gttgtaatcg catccatgat ataagacgca tattcgcgac
     4921 ttcttgtgtg ttccaacgaa ccgtcattga cgatctcgtc tctttgagtt tcccagcctg
     4981 cgatttgatc aacgcatctt ctcaagtatt catcgatcgg gatctgcaat tgatcgatca
     5041 attccggata attctttttg atgaagtaag gatggtactc tgcgttatgc tcgctggatt
     5101 ctgtgatgta gtagccgaag tgtttcatca attcaaagcg cacagaatcc ttatgcggat
     5161 tggctatttc tgaagccagt ctgcggattt cagggtagaa atcttctccg ttccggttaa
     5221 tttccagcag ccaagccatg tggttgatgc cggcgatttt ccattggaat tcatccagat
     5281 tgtattgatc tttaatgccc agatgttcga ataattccgg cacacagact tggacactgt
     5341 ggcacaagcc gaccgttttg atcttcgtcg ctttcagcat gcccatcgtc aaaatggcca
     5401 tcggattggt gtagttcaat aaccatgcat ccggacaaac ttcttccatt tctttggcga
     5461 tgtcaaacat caccggaagt gtgcgcaatc ctctgaagat cccgccgata ccggtagtgt
     5521 ccccgattgt ctgttgcaag ccgtattttt taggaatttc aaaa
//
LOCUS       DAOMUJ010000063         5528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4049_length_5528_cov_1.796821, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000063 DAOMUJ010000000
VERSION     DAOMUJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_5528_cov_1.796821"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>469
                     /locus_tag="PLT73_02675"
     CDS             <1..>469
                     /locus_tag="PLT73_02675"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214116.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRM19318.1"
                     /translation="HYGSPESIEFTDIYFMLLNYYTLAASNKIAKERGQSFVNFEKSK
                     YYTGEYFDAYTDTDVVFQSEKVKQIFEGIKVPTKEDWLQLKQAIHESGLYHQNRLAIA
                     PTGSISYVNETSASLHPITRLIEERQEKKTGKTYYPAPQLSNETMPYYRSAYDI"
     assembly_gap    470..479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <480..743
                     /locus_tag="PLT73_02680"
     CDS             <480..743
                     /locus_tag="PLT73_02680"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214116.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRM19319.1"
                     /translation="MRRVIDIYVAAQKHIDQGMSLTLFMRSELPEGMYEWKEGRTNKM
                     TTRDLNILRNYAWKKGAKSIYYVRTFTENNDEIGANACESCTI"
     gene            810..1775
                     /gene="nrdF"
                     /locus_tag="PLT73_02685"
     CDS             810..1775
                     /gene="nrdF"
                     /locus_tag="PLT73_02685"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HRM19320.1"
                     /translation="MANKPYIAINWNSIEDMLDKLTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSKAEQDMLGKVFGGLTLLDTLQSQDGIAAMKEDMRTQHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKAEIEDIFNWTNSNEFIQRKASLINDIYKSGNQLEKKAASVLLESF
                     LFYSGFYAPLWYLGNNKLPNVAEIIKLILRDESVHGTYIGYKFQIAYNQLSETEQEEV
                     KNWVYMLTYELYNNEVKYTQYIYDELGWTERVKVFIRYNANKALQNLGFDPLFPDTAD
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGAVEAMEDEDYVKWID"
     gene            2167..3231
                     /locus_tag="PLT73_02690"
     CDS             2167..3231
                     /locus_tag="PLT73_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM19321.1"
                     /translation="MRLSNKRLFAGLALSSMLVLSACGTGAATTAEAEVDLNALTLSE
                     IETQAKEEGHVESVGMPDTWANWGETWDELETTYNLTHADTDMSSAEELALFKAEQKN
                     PTKDIGDVGQSFGPVAEADGLTLAYKTSYWDDIPEWAKDDDGDWIVGYYGTLAFITNS
                     EKVTDIPTSFADIMEGDYKVTIGDVNAATQAQNAVLAAAIANGGDETNLDPGIAFFAQ
                     LAEQGRLDLGDTSLARLESGEIEVGLFWDFNALNYANQVSETNPNASFEVTVPLDGTI
                     QSGYATVINSTSPNPHAAALAREYILSDEGQINLAKGYARPIRDNVELPQEVQDILLP
                     EEQYVKGLQPAWAKDGKKKY"
     gene            3261..4109
                     /locus_tag="PLT73_02695"
     CDS             3261..4109
                     /locus_tag="PLT73_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRM19322.1"
                     /translation="MDKVIFVVLDGLNAKTAHNHMGFLEHLVEKGIMAAYTIKSEMPA
                     QSRPLYEVLQTGVPAAENGITSNRTVRRSKEQSVFEIAKDNGLKTGAAAYFWVSELYN
                     RAPFQMMEDRIQLDTNALIEHGIFYHEDHYPDNHLIADGDYLIRKKATDYTLIHSMNI
                     DDAGHKFGADSKEYVQAAVRVDAELSQYIWRWMSEGYQIVVTSDHGMNDYHTHNGISD
                     EDRLVPLYLFSDKVIVKDFRKEEAVVPQLEIAPFLCNLLGIPAGDRMQAVQGILMKRG
                     SGDAAE"
     gene            4096..4941
                     /locus_tag="PLT73_02700"
     CDS             4096..4941
                     /locus_tag="PLT73_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM19323.1"
                     /translation="MLQNEKRKYLILLLPFALLILLFELMPLANIFINSFRNPATGSV
                     TFANFTTLFTSEYYLMSIKNSLFVSLLSAFIGIALALLGAYAIHTAGDRMKKFYITTL
                     NMTSNFQGIVLAFAFILLLGNSGILTTLGEKWNLAVLNNYDLYTTSGILITFIYFQIP
                     LATLLLYPSFDGIKTEYKEAASLMNATAWQFWTKIGLPLVLPSIFGTFSVLFSNALAA
                     YATPYALLGNNYALLPIRISSMFTGDVVQQVELGSALSIVMLVLMSTMTVLSNSLLKK
                     FRKGV"
     gene            4945..>5528
                     /locus_tag="PLT73_02705"
     CDS             4945..>5528
                     /locus_tag="PLT73_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19324.1"
                     /translation="MKKRSTPFSSFIIVILSVILLLPLFVTLLNSLFRDLSNIIPEGF
                     TFEFYSGIFVESGGMAGAIGRSLLIAIIPTLVLLLLLLLAMYVVQIHYPKWDKYLDLL
                     SKIPYGIQGVILAVSIISLYASSSTFLSNRIFLLSCAYGIVILPYMYQGIKNALTTLE
                     VMPILEAAETLGSSRLYAYFRIIVPSVLKGLVAT"
BASE COUNT         1514 a         1239 c         1362 g         1403 t
ORIGIN      
        1 gcattacggt tcgcctgaat ccatcgaatt cacggacatc tactttatgc ttttgaacta
       61 ttacactttg gcagccagca acaagattgc taaggaacgc ggacaatcat tcgtcaattt
      121 cgagaagtcc aaatattata cgggcgaata ttttgatgcc tatactgata ctgatgtcgt
      181 tttccaatcc gaaaaagtca aacaaatctt tgaagggatc aaggttccga cgaaagaaga
      241 ctggttgcag ttgaaacaag caatccatga atccggcttg tatcaccaaa accgtttggc
      301 gattgcgccg accggttcga tcagttatgt aaatgaaacg agtgcaagtc tgcacccgat
      361 cacgcgtctg attgaagaac gtcaagagaa gaaaaccggt aagacttatt atccggctcc
      421 gcagctttcc aacgaaacaa tgccgtatta tcgttcggcc tacgatatcn nnnnnnnnna
      481 tgcgccgtgt catcgatatt tatgtcgctg ctcaaaagca catcgaccaa ggcatgagcc
      541 tgacgttgtt catgcgctca gaattgccgg aaggtatgta tgaatggaaa gaagggcgca
      601 caaacaaaat gacgactcgc gacctgaaca tcctgcgcaa ttatgcctgg aaaaaaggcg
      661 caaaatcgat ttactatgtg cgtactttta cggaaaacaa tgacgaaatc ggagcgaacg
      721 cttgtgaatc atgcaccatc tgatgattca acgcactgta tttgattact cttatgcaat
      781 aatcggaaag tctaacggag gaatgaagga tggcaaacaa gccttatata gcaatcaact
      841 ggaacagcat cgaagatatg ctggacaagt tgacatggga aaaactgacg gaacaatttt
      901 ggctggatac gcggatcccg ttgtccaatg atttggacga ctggcgcacg ctcagcaaag
      961 cggagcagga tatgttgggg aaagtgtttg gaggattgac gcttttggac acgctgcaat
     1021 cgcaggacgg catcgccgct atgaaggaag atatgcgcac gcaacacgag gaagcggtat
     1081 tgaacaatat ccaattcatg gaatcggtgc acgcgaaaag ttattcctcg atcttcagca
     1141 ccttgaatac gaaggctgaa atcgaggaca tcttcaattg gaccaacagc aacgaattta
     1201 tccaacgcaa agccagtctg atcaacgata tctacaaatc cggcaatcag ttggaaaaga
     1261 aagctgccag cgtgctgttg gaatctttct tgttctattc cggattttat gcaccgcttt
     1321 ggtacctcgg caacaacaaa ttgccgaacg tcgcggagat catcaaactg atcctgcgcg
     1381 acgaatcggt ccacggcacg tatatcggct ataaattcca gatcgcttac aaccaactgt
     1441 ccgaaacgga gcaggaagaa gtcaagaact gggtctatat gctgacgtat gaactctaca
     1501 ataatgaagt gaaatacacg caatacatct acgacgagtt gggctggacg gagcgcgtca
     1561 aagttttcat tcgctacaat gcaaacaaag ccctgcagaa cctgggcttc gatccgttgt
     1621 tcccggacac ggccgacgat gtgaatccga tcgtcatgaa cggtatctcg accggcacca
     1681 gcaaccacga cttcttctcg caagtcggga atggctacct gttgggtgcc gtcgaggcga
     1741 tggaagacga agattacgtc aaatggattg actaaactga aaagcaagga gagcagcgga
     1801 tcgattcgcc gctctccttg ctttttttgc atatgtgcct cgacaatttt agtgttgctt
     1861 gctgaggcag gttccggaac catggataac tttctattat tcatgtttta gctcgtggtt
     1921 gggtctgaaa gcatgaatta atttgcgtta ttcatgtttt atctcgtgat tgcgtctgaa
     1981 tgaacagaag ctagtgctat ccaatttttt cgaaaggaat gctgcaaccg catggtttca
     2041 ttgccgttta caaaaggtta acaacgaatc caactgttct ttacatccgt ctgttagtat
     2101 ttatttgtag tcaaatacgt aacgaaaaag aatgaaaaat tcattcataa ataaggagag
     2161 aataaaatga gattgtccaa caaaagattg tttgccggtt tggcattgag cagcatgctt
     2221 gttttgagcg catgtggaac gggagcagcg acaactgctg aggcggaagt cgatctgaac
     2281 gccttgacat tgagtgaaat tgaaacacaa gccaaagaag agggccatgt ggaatcggta
     2341 gggatgccgg atacatgggc caactggggc gagacttggg acgaactcga aacaacttac
     2401 aacttgacgc atgcggacac ggatatgagt tcagctgaag aattggcttt gttcaaagcg
     2461 gaacagaaaa atccgacgaa ggatatcggc gatgtcggcc aatctttcgg acctgttgcc
     2521 gaagcggacg gcctgacttt ggcctacaaa acatcttact gggatgatat tccggagtgg
     2581 gcgaaggatg atgatggcga ttggatcgtc ggttattacg gaacattagc gttcatcact
     2641 aattctgaaa aagtaacgga tatcccgacc agcttcgcgg atattatgga aggcgattat
     2701 aaagttacga tcggtgatgt aaatgccgca acccaagcac aaaatgcggt actggctgca
     2761 gccatcgcaa atggcggaga tgaaaccaac ttggatccag gcattgcctt ttttgcgcaa
     2821 ctggctgaac aaggcagatt ggacctgggc gacacttcat tggcccgttt agaatcaggc
     2881 gaaatcgaag tcggcttgtt ctgggacttc aacgcattga attatgcgaa ccaagtttcc
     2941 gaaacaaatc cgaatgccag ttttgaagtg actgttccgt tggatggaac gatccaaagc
     3001 ggatatgcaa cagtcatcaa ttcgacgtct cccaatccgc atgctgcagc cttagcccgc
     3061 gaatatatcc tttctgatga aggacagatc aatttggcga aaggctacgc acgtcctatc
     3121 cgtgataatg tcgaattgcc gcaagaagtg caggatatct tgcttccgga agagcaatac
     3181 gtgaaggggc tgcagccggc ctgggccaaa gatggcaaga agaagtactg acaaaagtaa
     3241 aataatcgag gttgaataga gtggataaag taatttttgt agtgttggac ggattgaatg
     3301 ccaagacagc ccataaccat atgggcttct tggaacatct ggtcgaaaaa ggaatcatgg
     3361 cggcttatac catcaaatca gaaatgcccg cacaatcaag gcccttgtat gaagttctgc
     3421 agactggtgt gccggctgcc gaaaatggca tcacctcgaa tcgcacagtc cgtcgttcca
     3481 aagaacagag tgtttttgaa atcgccaagg ataacggact caagacgggg gcggccgctt
     3541 acttctgggt aagcgagctg tataaccggg caccctttca aatgatggag gatcgcattc
     3601 aactggatac gaatgctctc atcgagcatg gcatttttta tcatgaagat cattacccgg
     3661 acaatcatct gattgcggat ggcgattact tgatccggaa gaaagcgacg gattatacgc
     3721 tgatccattc gatgaacatc gatgatgccg gccataaatt cggcgccgac agcaaggaat
     3781 atgtacaggc ggccgtccgc gttgatgctg aactttctca gtacatctgg agatggatga
     3841 gcgaaggcta tcagatcgtg gtcacttccg atcatggcat gaatgactac catacacata
     3901 acggtatttc tgacgaagat cggttggttc cgctctattt gttttcggac aaggtaatcg
     3961 tcaaagactt ccgcaaagaa gaggcagtcg tcccgcagct cgagattgcg ccatttttat
     4021 gcaatttgct gggcattccg gccggagatc gcatgcaggc ggttcaggga atccttatga
     4081 aaagagggtc tggcgatgct gcagaatgaa aaacgcaagt atctcatatt gctgctgccg
     4141 ttcgcgctgc tcatcctcct gttcgaattg atgccgttag ccaacatctt catcaacagc
     4201 ttcaggaatc cggcaacagg cagcgttacg ttcgccaatt tcacgacctt gtttacgagc
     4261 gaatactact tgatgtcgat caagaacagc ctgttcgttt ccttgctttc cgcattcatc
     4321 gggatcgcgt tggcgctcct gggtgcctat gccatccata cagcgggcga caggatgaaa
     4381 aagttctaca tcaccacttt gaacatgact tcaaacttcc aaggaatcgt attggcgttt
     4441 gcgttcatcc tgttgttggg gaattcaggg atactgacga cattgggtga gaaatggaac
     4501 ttggctgtat tgaacaacta cgacctgtac acaacctcag ggatcctcat caccttcatt
     4561 tatttccaga ttcctttggc gaccttgttg ctgtatcctt cgtttgacgg catcaaaacc
     4621 gaatacaagg aagcggcgag cctgatgaat gcgacggcat ggcaattctg gacaaaaatc
     4681 ggcctcccgt tggtgctccc cagcattttc ggaacctttt ccgtcctctt ctccaatgcg
     4741 ctggcagcct atgcgacacc gtatgctctg ctcgggaaca attatgcctt attgccgatc
     4801 cggatttcat ccatgttcac aggtgatgtc gtccaacagg tggagttggg aagtgccttg
     4861 tcgatcgtga tgctcgtatt gatgagcacg atgacggttt taagcaacag cctgttaaag
     4921 aaattcagaa agggtgtgta aaaaatgaag aaacgaagca cgcccttttc gtcattcatc
     4981 atcgtcatcc tgtccgtgat tttactgctg ccactgtttg tgacgctatt gaattctttg
     5041 ttcagggatt tgagcaacat catcccggaa gggttcactt ttgaatttta tagcggaatt
     5101 tttgtggagt caggcgggat ggcgggcgcg ataggcaggt ctttgctgat cgcgatcatt
     5161 ccgacgttgg ttctattgct gctgcttttg ttggcgatgt atgtcgtgca gattcattac
     5221 ccgaaatggg ataagtattt ggacctattg tcaaaaatcc cttacggcat ccaaggggtc
     5281 atccttgcgg tatcaatcat ttcgctctat gcctcttcca gcacctttct gtcgaatcgc
     5341 atttttttat tgagctgtgc ctacggaatc gtcattcttc cctacatgta ccaaggcatc
     5401 aaaaacgctt tgaccacact ggaagtcatg ccgattctgg aagccgcgga aacgttgggc
     5461 tccagtaggc tttatgccta tttccggatc atcgtaccta gcgtgttgaa gggactggtt
     5521 gcgacgat
//
LOCUS       DAOMUJ010000064         5521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4055_length_5521_cov_3.088547, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000064 DAOMUJ010000000
VERSION     DAOMUJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_5521_cov_3.088547"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLT73_02710"
     CDS             complement(<1..1129)
                     /locus_tag="PLT73_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRM19325.1"
                     /translation="MTQQIFEINPWKVTTKQLDKENRRLQESLTSLGNGYMGMRGNFE
                     EAYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINAMNFLYMHIFVDGEEIDL
                     YTDEVKDFDLTLDMEKGRLERFFTVVKNGKEITVHFTRFLSIDIKELCAIKVEITASE
                     KAAIRIESALDGNVQNEDANYDEMFWEWVEQTDDTLVVETIPNNFGIERFSVAAAMHH
                     KATGFNQKGNNSKELFVSQVFEGEAGNGQVLSLEKYVTLTTSRDHAKDQLAATAEEIY
                     ATKVAGQSFEELEAKQANLWTKRWDLADVEIGGDDEAQQGIRFNLFQLFSTYYGEDER
                     LNIGPKGFTGEKYGGATYWDTEAYGVPFYLAVTEPEVTRNLL"
     gene            1389..3050
                     /locus_tag="PLT73_02715"
                     /pseudo
     CDS             1389..3050
                     /locus_tag="PLT73_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
     gene            3493..4311
                     /locus_tag="PLT73_02720"
     CDS             3493..4311
                     /locus_tag="PLT73_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRM19326.1"
                     /translation="MKLLTLNTYSWVQTADPETYAALIADILENEYDAIALQEVNQLS
                     SGTSVNEPEGYLPTQNEIPIKSDNFAYFLVKSLAEKGLSYHWSWVPCHQGYFRFDEGV
                     AILSKAPIQSVEQVQLSVVNDFESIHTRRALVLETEAAAFVSVHLSWWKTEEENPFLL
                     EWTSVETAVKKLRGIKPIYVMGDVNNPADVRNEGYDLITNAGWQDAYAVAATRIGSAT
                     VPPAIDGWEGNEAPLRIDYIFSDQPQAVETYEVKFDGKKLPCVSDHYGVAVTYA"
     gene            complement(4486..>5118)
                     /locus_tag="PLT73_02725"
     CDS             complement(4486..>5118)
                     /locus_tag="PLT73_02725"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HRM19327.1"
                     /translation="KLFGDAMTDYAGRIMASICGNDVASIKQLVEDEEVDKYIKVEAL
                     TALAILTLNGELERQELMAYYKELLPTIDNPTILTLLINLCTDIYPGEVYDEIKEAYK
                     NDKVDSFLIGMGSVDQAMVEGQSMVLYRAERDRNLQKIDDTIGEMRNWAYFENEEDSA
                     EENDFEQLTNNNLNFVHQPKVTPIVNEPKIGRNDPCPCGSGKKYKKCCGK"
     assembly_gap    5119..5128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5129..5481)
                     /locus_tag="PLT73_02730"
     CDS             complement(<5129..5481)
                     /locus_tag="PLT73_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HRM19328.1"
                     /translation="MVSRELKETVLEAVDNQLNDNDPKCTTGTFGHSEKTMAELIDRI
                     KCDPDRIFPEEELAEIIARKEEAIPLLMAFLEEVRDNAEQFSNNFDYLGHIYAVLLLA
                     QFRVKEAYPIVLELF"
BASE COUNT         1304 a         1415 c         1260 g         1532 t
ORIGIN      
        1 tcaagagatt gcgggtaact tccggctcgg taaccgccaa gtagaacggc acgccgtaag
       61 cttccgtatc ccagtaggta gcgccgccgt atttctcgcc ggtgaagcct ttcggtccga
      121 tgttcaggcg ctcatcttcg ccgtaataag tagagaacag ttggaacagg ttgaaacgga
      181 ttccttgttg cgcttcgtca tctccgccga tttcgacatc cgccagatcc caacgcttag
      241 tccacagatt cgcttgtttt gcttccaatt cttcgaaact ttggccagct actttggtgg
      301 cgtagatttc ttcggcagtt gctgccagct ggtccttcgc atgatcgcgg ctcgtcgtca
      361 gcgttacgta tttctccaag gaaaggactt ggccgtttcc agcttcccct tcgaacactt
      421 gggagacgaa caattctttg ctgttgttgc ctttttgatt gaagcctgtc gctttgtggt
      481 gcatcgctgc agccacactg aaacgttcaa tgccgaagtt gttcgggatc gtttcgacga
      541 ccagcgtatc gtccgtttgt tcgacccatt cccagaacat ttcatcatag ttggcatctt
      601 cgttttggac attcccgtcc agagcacttt cgatacggat cgcggccttt tccgaggctg
      661 tgatttccac tttgatagcg caaagttcct tgatgtcgat gctcaggaag cgggtgaaat
      721 ggacggtgat ttctttaccg ttcttcacta ccgtaaagaa gcgttccaag cggccttttt
      781 ccatatccaa cgtcaagtcg aagtctttca cttcatcggt gtaaagatcg atttcttctc
      841 cgtccacaaa gatgtgcata tacagaaaat tcatcgcatt gatcactttt ccgaaatatt
      901 ccgggtagcc gtttttccac cagccgacgc gtgttttgtc gggataccag acgcccgcca
      961 gatacgtgcc ttgatggtga tcgcccgaat aggcttcctc gaagtttcca cgcatgccca
     1021 tgtagccgtt gccgagactg gtcagacttt cctgcaggcg gcgattttct ttgtctaatt
     1081 gtttggttgt tactttccaa ggattgattt caaaaatttg ttgcgtcatt attggttccc
     1141 ccttatttgt tcttccccct tagtttacat gcaatcggtt gcattgtcaa tgcaaaagtt
     1201 gcattattat ttccgacgca tgatttcctt ggcattgaaa cgttttcttc atctatccgt
     1261 ttaaatatcc gtttaaaaac caatttattc tgaaatagac cgcttgcaaa atttctggat
     1321 atggtctata cttcgaatcg taaacgttgc aatcgattgc accgataaat ttgagaggag
     1381 ttttaaaaat gaagaaaata tttacattcg atttttggca aaaatttggc aaggctttga
     1441 tggtcgttat tgcggtcatg ccggcagctg gtttgatgat cagtatcggc aagatgatcg
     1501 ctatgttctc tggagatgtc caattgttac tgacaacggc tggggtcatc gaagatcttg
     1561 gctgggctgt catcgtcaac ctgcatatcc tgttcgctgt cgccatcggg ggttcctggg
     1621 cgaaagaacg tgccggcgga gccttcgctg ctttgcttgc cttcatcctg atcaaccgca
     1681 cgaccggtaa acttttcgca gtgaccggag atatgttggc tacggacgga gcgactacca
     1741 aaaccttgtt cggccaggac atcctggtga acggctactt taccaatgta ttgggcgcac
     1801 ctgctttgaa tatgggtgtc ttcgtcggga tcatcgccgg gtttgtcggc gccgtcatct
     1861 tcaacaagta ctacaactac cgcaaattgc ctgatgcatt atctttcttc aacggcaaac
     1921 gtttcgtgcc tttcgtcgtt atcggctggt ccgtactcat ttcccttgcc ttggcaatca
     1981 tctggccagt cgttcagacc ggcatcaata atttcgggat ttggatcgcg gactccgcgg
     2041 ataccgcacc gattttggcg ccgttcgtat acggtacact ggaacgtctg ttgttgccgt
     2101 tcggtctgca ccatatgctg acgattccga tgaactacac ttctttgggt ggaacttaca
     2161 ccattttgac aggcgctgct gcaggttctc aagtgttcgg tcaagatccg ctttggttgg
     2221 cttgggccag cgatttggtg aacttgaaca acaccggcga tacagcagcc tacaatgagt
     2281 tgcttaacgc attcactcct gcccgcttta aagttggtca aatgatcggc gcagccggca
     2341 gcttgatggg cttggcttat gctatgtacc gcaacgtcga tgtcgacaaa cgtccgaaat
     2401 acaaatccat gttcttctcc gcagccatcg ctgttttcct gacaggggta accgaaccgc
     2461 ttgagtacat gttcatgttc gctgcaccgc tgctttacgg cgtttacgca gttctgcaag
     2521 gggtcgcatt tgcaatggcc gacatcatca gcctgcgcgt ccactccttc ggtacactgg
     2581 aattcctgac acgcatcccg atgtctttga aagccggctt atggttggat gtcgtcaact
     2641 acatctgggt gacggtcgcg tttgctgtca tcatgtactt cgtcgccaac ttcatgatca
     2701 aacgcttcaa attcgcaacg ccaggccgtt tgggcaacta cacggacttc gacggcgaag
     2761 cggaagaagc tgctcctgcc actgcaacac cggaaggcaa cgctggtgca tccgataacg
     2821 ataaactagt ttggaacatc atcgacgcat tcggcggcca agaaaacatc actgaagtcg
     2881 atgcttgcat gacccgtctg cgcgtcaccg tcaaagacga aacgaaagtg gccgaagaca
     2941 aaaaatggtc cacattgggt gcacgcggcg tcatccgcaa aggcaaaggt atccaagtcg
     3001 tgtacggtcc gcaagcagat gtcctaaaat ctgatataat agatctatta ggaagatagg
     3061 aaaagcggaa cgggttcgtt cagccctgat taaggaaaca ctgtgaccta atcggtcaag
     3121 tgtatcatgc ctctaagcga ttgaaatcgc aagagtcatg acaatttagg aaatctgtcc
     3181 acgcaacgtt ccctacggtc accttgtact gggactctaa gggatgaatc cctaagagtc
     3241 ccatgcaact gagcatcgta gagcgcaata tgctgagaga cttcctgcgt cagcaggtta
     3301 gtcgaacagt atccttggct cgaagagaca aggggttcct tcataaataa tttccgaagg
     3361 gcttacccgt gtagctagcc atcattttgg atgcacgaaa cactttaaaa gtctgtaaaa
     3421 catcccaaat gatgcacttt ctattttaaa agattatctt aataactata tatttagaat
     3481 ggagaggacc tcatgaaact actgacattg aatacgtaca gctgggttca aacagcagac
     3541 cccgaaactt acgccgccct gattgcggac attctggaaa acgaatacga cgctatcgcc
     3601 ctgcaggaag tgaaccagct ttccagcggc acaagcgtca acgaaccgga gggctatctg
     3661 cccacccaga acgaaatccc gatcaaatcg gataacttcg cctacttctt ggtgaagtcc
     3721 ttggcggaga aaggactttc ctatcattgg tcctgggtac cttgccacca aggctacttc
     3781 cgtttcgatg aaggagtcgc catcctcagc aaagcgccga ttcagtcagt cgaacaggtc
     3841 caactttctg tagtcaatga tttcgaaagc atccacaccc gccgcgcttt ggtcctcgag
     3901 accgaagcag ccgctttcgt ctccgtccac ctgtcctggt ggaaaacgga agaagaaaat
     3961 ccgttcttgt tggagtggac ttccgttgag acggctgtca aaaaactgcg cggcatcaag
     4021 cccatctatg tcatgggtga cgtcaacaac ccagccgacg tccgcaacga aggctacgac
     4081 ctgatcacga acgccggctg gcaggatgcc tacgccgtcg ctgcaacgcg tattggatct
     4141 gcgactgttc cgccggcgat cgacggttgg gaaggaaacg aagccccgct gcgcatcgac
     4201 tacatcttct cagaccagcc gcaagcggtc gaaacgtacg aagtcaaatt cgacgggaag
     4261 aaattgcctt gcgtgtctga tcattatggg gttgcggtga catacgcctg agattgatac
     4321 atttgaaaga aagaagatct acctcgcgcg aggtagatct tctttttaca tttagttttg
     4381 ttggacccag tggcgggggt gccgcgaaat gcggaattag cccgccaaaa gcgttttgac
     4441 gggctaattc acttcgtcac ctacattctt atatcgtttt tccacttact tcccgcagca
     4501 tttcttgtat ttcttgccgc tcccgcaagg acaagggtca ttgcggccga tttttggttc
     4561 attgacgatt ggcgtcacct tcggttggtg cacgaaattc agattgttgt tggtcagctg
     4621 ctcaaaatcg ttctcttcgg cagaatcttc ttcattttca aaatacgccc agttcctcat
     4681 ttcaccgatc gtatcatcga ttttttgtaa gtttctatct ctctcggctc tgtacagtac
     4741 catcgactgg ccttccacca tggcctgatc tacggatccc ataccaatca ggaaagaatc
     4801 caccttatca ttcttatagg cttcctttat ttcatcatac acctcgcccg ggtagatatc
     4861 cgtacagaga tttatcagca acgtgagaat ggttggatta tcgatcgtgg gaagcagttc
     4921 tttgtaataa gccataagct cctgccgttc gagctccccg ttcaatgtca aaatagccaa
     4981 ggcggttagc gcttcaactt tgatatactt atcgacttcc tcgtcctcaa ccagttgctt
     5041 tatggatgcc acatcatttc cgcaaatgga tgccattatc ctcccggcat aatctgtcat
     5101 cgcgtctcca aacaacttnn nnnnnnnnct gaacagttcc agcacaatag gataagcttc
     5161 cttgactctg aactgagcca acagaagaac cgcataaata tgtcctaaat aatcaaagtt
     5221 gttgctgaat tgttccgcat tatccctcac ctcttcaagg aaagccatca ataaaggaat
     5281 cgcttcttct tttctggcaa taatttccgc caactcttcc tcagggaaaa tcctgtcggg
     5341 atcacatttg atgcgatcaa ttagttcagc catcgtcttt tcggaatggc caaaagtacc
     5401 tgtcgtgcat tttggatcat tgtcgtttaa ttgattatcc actgcttcca acacggtttc
     5461 ttttaattct ctgctcacca tcactaacac ctcaccaatg atataggttt ttgtcactgc
     5521 t
//
LOCUS       DAOMUJ010000065         5392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4183_length_5392_cov_2.313285, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000065 DAOMUJ010000000
VERSION     DAOMUJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4183_length_5392_cov_2.313285"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLT73_02735"
     CDS             <1..184
                     /locus_tag="PLT73_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19329.1"
                     /translation="GEYQTVCSLNTYELIRPKNAPTVSTIQYHWDGKVKTLLLPDEGD
                     LFENVGPESAGGGQAG"
     gene            144..1436
                     /locus_tag="PLT73_02740"
     CDS             144..1436
                     /locus_tag="PLT73_02740"
                     /inference="COORDINATES: protein motif:HMM:NF023224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HRM19330.1"
                     /translation="MSVRKVLGVAKLDENLKEEAIRYFAGKPVMVICIKQIHKKYESF
                     GRFTGTISKSLFKKVDAEPLLMFMGIAQWEWEKSKSIKISDFKRNFELVVESISGRQL
                     RTKQELADDAEAEYAAFLAEIDGIDPLFREVFGRNRNAVYSGWFLKEPVACLAAFRNV
                     AEALHNLPQEYTKLPVFAHRITGNPHSFDSNGLTGQLLFMMLYHQYAEADSAQAMNGL
                     LSKAELENEIYGLFKIIKDDIMNFTAVNGLVAFRGEEPVAMWQDACLDRIPWNVPVRQ
                     LLGISRIRPCKGNQIFLIENSGVYSILLDAFPDCPMVCTNGQFRYAVWLLLERIPDDG
                     ITLYYSSDFDPEGLLMADTLKRRYGEKLQLLGMTIDHYSASKPAAEIDEKRMQKLKRI
                     QSGELRGLADLMAEKKFAGYQEGILEELLDEIRLLRED"
     gene            1598..1810
                     /locus_tag="PLT73_02745"
     CDS             1598..1810
                     /locus_tag="PLT73_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19331.1"
                     /translation="MIIWLTSDKTNKEYTEYNVYKRGILMGGISHHKDDDFWQWWVEG
                     VGMKKTRKMYKEATEDAAKRAVEYEI"
     gene            complement(2121..3140)
                     /locus_tag="PLT73_02750"
     CDS             complement(2121..3140)
                     /locus_tag="PLT73_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM19332.1"
                     /translation="MNEVFDVTIIGGGPSGLYSAFYSGLRNLKTKLIESQPVLGGKVN
                     LYPDKILWDVGGLTPITGKQFLSNCITQGLTFHPTVCLNTEVSLITKVAGVFDIRASN
                     GAHHYSKAVILAYGSGIITPNKLEVDGAEQFERTNLHYIPSSLDAFRNKEVFLSGGGN
                     TAIEQANELLSIANGVTLAYRSNELRAHEHSVESFCNNGGTLLLETQIRSFHSNRQGE
                     IEAVSLSVGGEHRTVVSNAVIVNHGFASKQAITFGDVVELTFNENNFIEGSPDSTIGH
                     PGIFAAGDAIAYTGKVNLLVGTFQDAVNAVNSVKQYLNPTAEKHAMVSSHNEAFVEKN
                     KQFSL"
     gene            3308..4102
                     /locus_tag="PLT73_02755"
     CDS             3308..4102
                     /locus_tag="PLT73_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19333.1"
                     /translation="MCSLKVEEVQIKYDKQIIVKNISVTIAPQKITTIIGPNGCGKST
                     LLKAMSRILTCSSGDILLDGVSIFTMDTKRLAKRIAILLQRQENLSGITVEEIVSYGR
                     FPHRKGLRGLGSEDKEIIDWAIKKTQIDELRHRYIDELSGGQRQRVWLAMALAQKTDI
                     IFLDEPTTYLDISYQLEILELLKKLNEEEGYTIVMVLHDINQASLYSDHIVALADGKV
                     VCQGAADKVITAANIASIFNIRPTIQPDAKTNKPVIIGYELINRGE"
     gene            4105..4560
                     /locus_tag="PLT73_02760"
     CDS             4105..4560
                     /locus_tag="PLT73_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19334.1"
                     /translation="MKKINKLSLTMLLSAGLILGACSATDTSDETSESAAADTVTYTT
                     VDGEEIAVPANPERVVVLSSYAGDLINFDINIVGVDAWSAGNPNFSEGLSGVAVVSNA
                     DVEKILELEPDLIIGLDNIENADQLSEIAPSCSHTETFLTWIKSWKSGK"
     gene            4542..5030
                     /locus_tag="PLT73_02765"
     CDS             4542..5030
                     /locus_tag="PLT73_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19335.1"
                     /translation="MEIGKVVNKEEEAVAWVEEFQTEAQTVGEEIKAVIGEDATVTVA
                     ENFEKQMYIFGDNWARGTEILYQEMGLNMPETVKENALDAGYYAISEEVLGDYIGDYL
                     ILSLAATDPETSFLDAEWFQNIPAVQNNQVFVADAETFYFNDSLSLDYQLDFFEESFL
                     AE"
     gene            5046..>5392
                     /locus_tag="PLT73_02770"
     CDS             5046..>5392
                     /locus_tag="PLT73_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM19336.1"
                     /translation="MIKRKRKAFGIYLAVTVLLLIVAAVLALYTGAADTDWSDLFEAL
                     IGEGGGDYYLVLRGIRFPRILGAFFVGSSLAVAGAIMQALTRNPLADPGLLGLTSGAN
                     IAVALVYALAPQSS"
BASE COUNT         1410 a         1234 c         1491 g         1257 t
ORIGIN      
        1 cggggaatat caaacggtct gcagcctgaa cacatacgaa ctgatccggc ccaagaatgc
       61 gccgaccgtt tcgacaatcc agtaccattg ggacgggaaa gtgaaaacgt tgctgctgcc
      121 agatgaaggt gatctttttg aaaatgtcgg tccggaaagt gctgggggtg gccaagctgg
      181 atgagaatct gaaagaagaa gccatccgct actttgcggg caaaccggtg atggtcatct
      241 gcatcaaaca gatccataag aaatacgaga gcttcgggcg cttcaccggc acgatcagca
      301 agtcgctttt caaaaaagtc gatgctgagc cgttgctgat gttcatgggc atagcgcagt
      361 gggaatggga gaaaagcaaa agcatcaaaa tcagcgattt caagcgcaat ttcgagctgg
      421 ttgtggagag cataagcggg aggcaactca gaactaaaca ggaactggcg gatgatgccg
      481 aggcagaata tgctgcattc ctggcggaga tcgatgggat tgatccactg ttcagggagg
      541 tcttcggccg caaccgcaac gccgtttatt cgggctggtt cctgaaggag ccggttgctt
      601 gcttggctgc cttcaggaac gttgcggagg ctctgcacaa tcttccgcag gagtatacga
      661 aattgcctgt cttcgcgcac cgcatcaccg gcaatccgca ttcttttgac tcaaacggct
      721 tgaccggaca gttgcttttc atgatgctgt atcatcagta tgccgaagct gactcagctc
      781 aagcgatgaa cggcctcttg tcaaaagcgg aactggaaaa tgaaatctat ggactgttca
      841 agatcatcaa ggacgacatc atgaatttca ccgccgtgaa cgggctggtc gcctttcgcg
      901 gagaggagcc tgtcgctatg tggcaggatg cctgcctgga tcggataccg tggaatgtgc
      961 ccgttcgaca gcttttgggc atctcgcgga tccggccttg caaaggcaac cagattttcc
     1021 tgatcgaaaa ttcaggcgtg tattccatct tgctggatgc tttcccggac tgtccgatgg
     1081 tctgtacgaa cggccagttc cgctatgcgg tctggctgct gctggaacgg attccggatg
     1141 acgggatcac gctgtattat tccagcgact tcgatcctga gggcttgctg atggcggata
     1201 cgctgaaaag acggtacggt gaaaaactcc agttgctcgg gatgaccatc gaccactatt
     1261 cggcctcaaa accggcagcg gaaatcgatg agaaacggat gcagaagctg aaacggatcc
     1321 aatccgggga actacgcggt ctggcggatc tgatggccga aaagaaattc gccggttatc
     1381 aggaagggat tttggaggag ttgctggatg aaatcaggct tcttcgcgaa gattaataat
     1441 gatgttttgt gagctgcaag gaagcttgtt cacaatgtgt aacgcgtaaa gttcatgttg
     1501 aaaccttttt gatttagggg tagagttgac ttgttgaaaa ttttcaagca atcattcata
     1561 agattagaga caaaatcgaa cgagagaagg cgtgcagatg atcatttggc tgacatcgga
     1621 caaaacaaat aaagagtata ccgaatataa tgtctataaa aggggcatac tgatgggcgg
     1681 aatttcccat cacaaggacg acgatttctg gcaatggtgg gtggaaggcg tcggcatgaa
     1741 gaaaacccgc aaaatgtaca aagaagcaac cgaggacgca gccaaacggg ctgtcgaata
     1801 cgaaatctga cagaggaaaa gtggcgcagg gcagggatca ttcttggaat gagacttggt
     1861 ctgcgtttgt tttatttttg ggttgtcgac tgtttaggga agaataaatg cggaacagcc
     1921 gacaaacggt tcaggcgtcg actgttgtgg gaagaatgaa tgatgaatag tcaacaggta
     1981 ctcacttcgt cggctgatct gtaaggaacg aatgctgaac agccgacgaa aggtgccaga
     2041 aacgtaaaag cagccaaggg gactcccctc ggctgctttt ctacaaatct acaaatctac
     2101 aaatctacaa atctacaaat ctacaaacta aactgtttgt ttttttcaac gaaggcttcg
     2161 ttgtgcgagg acaccatcgc gtgcttttca gcggtggggt tcaggtattg tttgacactg
     2221 ttcacggcat tcacggcatc ctggaaggtg ccgacgagca gattgacttt gccggtatag
     2281 gcgatcgcgt cgcctgccgc gaagatgccg ggatggccga tcgtgctgtc gggactgcct
     2341 tcgataaaat tgttttcgtt gaacgtgagc tccactacat ccccaaaagt aatcgcctgt
     2401 ttgctggcga agccgtggtt gacgatcact gcgttggaaa ctactgtccg atgctcaccg
     2461 ccgaccgaga gggaaacggc ttcgatttcg ccttggcgat tagaatggaa ggagcgaatc
     2521 tgcgtttcca gaagcaatgt gccgccgttg ttgcagaagc tttctacgct gtgttcatgg
     2581 gcacgcagtt cattactgcg gtaagccaat gtcacgccgt ttgcgatgga cagcaattcg
     2641 ttcgcctgct cgattgctgt gtttccgccc cctgaaagaa aaacttcctt gttgcggaat
     2701 gcatccaggc tgctcggaat gtaatgcaga ttggtccttt cgaactgctc tgcaccgtcc
     2761 acctccaact tgttcggcgt gatgatgccg ctgccgtatg caaggatcac tgctttcgaa
     2821 taatgatggg caccattgct ggcgcggata tcgaaaacac ccgcgacttt ggtgatcagg
     2881 ctgacttccg tattcagaca gacggtcgga tggaacgtca ggccttgcgt gatgcaattg
     2941 gaaaggaact gtttgccggt gatcggtgtc agaccgccca catcccataa gattttgtct
     3001 gggtacaggt tgactttgcc gccaaggacg ggctgggatt cgatcagctt cgtcttcaga
     3061 ttgcgcaaac cgctgtagaa agcactgtac agacccgaag gcccgccacc gattatcgtt
     3121 acgtcaaata cttcgttcaa aagaccacct cattttctgt tgactaattg gatgttatga
     3181 atgataatag aataaattat aaatgagaat gattctcatt atcaattaaa taatttttat
     3241 tcgaaaatgt caagaacgat ccggaaatga atcgcttctg caaggataaa gaaaggggaa
     3301 tgaaggcatg tgcagcctga aagttgagga ggtgcaaatc aagtacgaca agcagatcat
     3361 tgtgaaaaat atctcagtca ctatcgcacc acagaaaatc acaacaatca tcgggccgaa
     3421 cggttgcgga aaatcgacct tattgaaggc gatgtcgcgt atcctgactt gctcgagcgg
     3481 cgacattctg ctggacggtg tttcgatttt tacgatggat acgaaacggt tggcgaaacg
     3541 gatcgcaatc ctgctgcagc gtcaggaaaa cctttccggc attacggttg aagagatcgt
     3601 ttcgtacggc aggttcccgc accggaaagg cttgagggga ttgggcagcg aggacaagga
     3661 aatcatcgac tgggccatca agaagacgca aatcgacgag ctgcgtcacc gctacatcga
     3721 tgaactgtca ggcgggcagc ggcaacgggt ctggctggcg atggcgttgg cgcaaaaaac
     3781 cgacatcatc tttttggatg aaccgacgac ctatctggac atcagttacc aactggaaat
     3841 tttggaactg ctcaagaaac tgaatgaaga ggaaggttat acgatcgtga tggtgctgca
     3901 cgacatcaat caggcttcgc tctattccga ccatatcgtg gcgctagcgg acggcaaagt
     3961 cgtctgtcaa ggtgcggcgg ataaagtgat caccgccgcg aatatagcgt ccatcttcaa
     4021 catccgtccg accatccagc cggacgcaaa gacaaataaa cccgtcatca tcggatacga
     4081 attgataaat agaggagaat gaacatgaaa aaaataaaca aactatcact gacaatgtta
     4141 ttgagtgccg gcttgatttt gggggcctgt tccgctacgg acacatccga tgagaccagc
     4201 gaaagtgcag cagccgacac agtgacgtac acaacggtcg acggcgaaga aatcgcggtt
     4261 cccgcaaacc cggaacgggt agtggtgctg tcgtcctatg ctggggatct catcaacttc
     4321 gacatcaaca tcgtcggcgt ggatgcttgg tcagccggta acccgaattt cagcgaaggt
     4381 ctttcaggcg tcgcagtcgt ttcgaatgcc gacgtcgaaa aaatcctcga attggaaccg
     4441 gatttgatca tcggcttgga caatatcgaa aatgccgatc aattgtcgga aatcgcgccg
     4501 tcctgttcac atacggagac ctttcttacc tggatcaaat cgtggaaatc gggaaagtag
     4561 tcaacaagga agaggaagct gtcgcgtggg tagaggaatt ccagaccgaa gcacagactg
     4621 ttggagaaga aatcaaggca gtaatcggcg aagatgcaac agtaacggtt gccgagaact
     4681 tcgaaaaaca gatgtacatt ttcggcgata actgggcacg cggcacggag atcctttatc
     4741 aggaaatggg cctgaatatg ccggaaacgg tcaaagagaa cgccttggat gcaggttatt
     4801 atgccatttc cgaagaggtg ctcggcgatt acatcggcga ctacctgatc ttgagcttgg
     4861 ccgcaaccga tccggaaacg agcttcttgg atgctgaatg gttccagaac ataccggccg
     4921 tgcagaacaa ccaagtgttt gtggcggatg ccgagacatt ttattttaat gattcgctat
     4981 ctttggatta ccaactggac ttcttcgagg aatccttttt agcggaataa gaggggacga
     5041 ttgcagtgat taaacggaaa cgaaaggcct tcggaatcta tctggcagtg actgtcttgt
     5101 tgctgatcgt ggcagcggtc ctagcgcttt acacaggagc ggcggatacg gattggtcgg
     5161 acctgttcga agcgctcatc ggggaaggcg gcggcgatta ttatcttgtc ctgcggggca
     5221 tccgcttccc gcgcatcctg ggtgccttct tcgtgggaag ttccttggct gttgccggag
     5281 cgatcatgca ggcgctgacg cggaatccgt tggccgatcc cgggcttttg gggttgacct
     5341 ccggagcgaa catcgcggtg gccttggtct atgccttggc cccgcagagt tc
//
LOCUS       DAOMUJ010000066         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4202_length_5372_cov_2.510062, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000066 DAOMUJ010000000
VERSION     DAOMUJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4202_length_5372_cov_2.510062"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLT73_02775"
     CDS             complement(<1..873)
                     /locus_tag="PLT73_02775"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRM19337.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKITIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFIDINLNQYSNVTTGKVYSEVIRKE
                     RKGDYLGATVQVIPHITNEIKEKIMRAGETTDSDIVITEVGGTVGDIESLPFLEALRQ
                     MRMEAGAENVLYIHTTLIPYLRAAGELKTKPTQHSVKELRSLGIQPNILVVRTEMPLP
                     QHIKNKLASFCDVKAEAVIESRDVETLYEIPLMLQEQNMDQIVCDYLGLTDLPQADMT
                     DWKALVEKVQSLEKT"
     gene            complement(1087..1695)
                     /gene="rpoE"
                     /locus_tag="PLT73_02780"
     CDS             complement(1087..1695)
                     /gene="rpoE"
                     /locus_tag="PLT73_02780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HRM19338.1"
                     /translation="MKLKQLDGTNKNELSMIEVAHAIFEENGDVLDFNQLLVQIQDYL
                     EMSEEELEGKMTRFYTDLNIDGSFISLGENRWGLRSWYPIDSIDEEILSSIDEDEVKV
                     RRKKRKKLNAFATSEDDDDVIDYNDDDPEDIDVADEDEEIDVDEDEVDDVDAVDITDD
                     DGDITSGIEEDLTLIIEDEEFEDEEELEGEEEFVAEETDDEE"
     gene            complement(1740..2165)
                     /locus_tag="PLT73_02785"
     CDS             complement(1740..2165)
                     /locus_tag="PLT73_02785"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HRM19339.1"
                     /translation="MSLKKGMPIEFQLETLVKQEGEQESFLYEGMGQIVEMGDWLYLR
                     YIEAETAIPVTFKLSREGKMTIIRRMEQTSRMTFDAEGKGMAMVPTPAGLMEIETITH
                     KMLLEFKEKPFAGKVSLHYELQMNEQKLGDYRIELQFTT"
     gene            2381..3232
                     /locus_tag="PLT73_02790"
     CDS             2381..3232
                     /locus_tag="PLT73_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRM19340.1"
                     /translation="MTNYLKNNIYHIYDTSNTAFGADPLAHFAMGDALIRLVGNAEAP
                     AALHFWPMEKLVILGMMDTRLPHLEDGLRYLRNVPTDIVVRNAGGLAVVADEGILNFS
                     LVLPETEKAKLTIHDGYVLMKELVEQAFSDSEQVIEAFEISNSYCPGDFDLSINGKKF
                     AGIAQRRFKNGVAIMIYMSVNGKQEQRGEMIRRFYEESLQGEETRWHFPAVDPGSMAN
                     LAELLDKPLTVADVKARILSVFTKNGNTLLPGAFNQRLMEEYEKAYTKMKKRNEKINQ
                     DHYPEAD"
     gene            3252..4637
                     /locus_tag="PLT73_02795"
     CDS             3252..4637
                     /locus_tag="PLT73_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM19341.1"
                     /translation="MSTHYTAELLPKEKVFRDPVHDYIHIQYRIIMDLINAPEFQRLR
                     RIKQLGTSSYTFHGAEHSRFNHSLGVYEIARRICDKFVRNYPSKAHGDGLWDDGERLV
                     TLCAALLHDIGHGPFSHTFEKIFDTDHEAITIEIITSPDTEINRILQRVGPDFPNQVA
                     AVIAKTYDNPQVVQLISSQIDADRMDYLLRDAYYSGVTYGNFDLTRILRVIRPYEHGI
                     AFDISGMHAVEDYIVSRYQMYMQIYFHPVSRGMEVVLSHLLKRAKDVYLDEQAEFRHT
                     ATFLTPFFKQTWTLKDYLRLDDGVLTTYFNHWLDEKDPILNDLAYRFVNRHPFISVRY
                     TKGRDDATLERMSAIIERMGFNPDYYTAVNNSYDLPYDLYRPHQATTKTQIELVQKNG
                     SFLELSQASSIVSALTGKVRGDERFYFPREITHGDEEAGINLFESLSKEFSTFFENDE
                     ILPTQQTGGTL"
     gene            4634..>5372
                     /gene="yidA"
                     /locus_tag="PLT73_02800"
     CDS             4634..>5372
                     /gene="yidA"
                     /locus_tag="PLT73_02800"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: protein motif:HMM:NF007806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HRM19342.1"
                     /translation="MSIKLIAIDIDGTLVDKEFKITPEVKAAITEAREQGVKIVLCTG
                     RPLPGVKRYIKELELDQDEDYVITYNGSLVLSTATNEVLVSHTLDYSDFVRINELADK
                     FHVHTHGIDSEAIYTANKDISIFTTRESFITTMPIRYRSLAELPEDKLFTKIMFIDQP
                     ELLDILIPAIPAQFHEDYVIVRSEPHFLEVLNKDAGKASALAELAALLNIDSENIMAI
                     GDNENDLSMIEYAGVGVAMGNAVDKVKE"
BASE COUNT         1387 a         1381 c         1185 g         1419 t
ORIGIN      
        1 agtcttttca agactttgga ctttttcaac caacgctttc cagtcggtca tgtcagcttg
       61 cggcagatcg gtcagaccaa gataatcgca aacgatctgg tccatgttct gttcctgaag
      121 catcaaagga atttcataca acgtttccac gtcccgagat tcgatgaccg cttcggcttt
      181 cacatcacag aaggaggcca gtttgttttt gatgtgttgc ggcaatggca tttctgtacg
      241 cactaccaga atattcggtt gaatccctaa gctacgcaat tctttgacgc tgtgttgtgt
      301 tggctttgtt ttcaattcgc cggctgcgcg caaataaggg atcaatgtcg tgtggatgta
      361 caatacattt tcagcaccgg cttccatgcg catttggcgc aatgcttcca ggaacggcaa
      421 agattcgata tccccaacgg ttcctccgac ttcggtgatg acgatgtccg aatctgttgt
      481 ttcgccggca cgcataattt tttctttgat ttcattcgtg atatgcggga tgacttggac
      541 agttgcccct aaataatccc ctttgcgttc tttacggatg acttcagaat aaactttacc
      601 ggtcgtcaca tttgaatatt gattgaggtt gatatctatg aaacgctcat agtggcctaa
      661 gtccaaatcg gtttccgtac cgtcatcagt gacgaaaact tctccgtgtt ggtaaggact
      721 catcgtaccc ggatccacat tgatgtaagg gtcgaatttt tggatcgtga ttttcaatcc
      781 acggttcttc agcaaacgcc ccaaagacgc cgcaacaata cctttcccga ttgaagatac
      841 taccccgcct gttacaaaaa tatactttgt cattttttct tccccctatt tttaaatgtg
      901 catcattata tctgaaggaa tctccaacgc tgcaataaaa aaataaagct ccctgttctt
      961 aaaagaacag ggagctgtaa aaattcaacg tatgctttcc aaaaagaatt tctttttggg
     1021 agcccaagga agataataca tccaattaca taaggtgtca agaaagaatt agataccgga
     1081 aaaggactat tcctcgtcgt ccgtttcttc cgccacgaac tcttcttccc cttccaactc
     1141 ttcttcatcc tcaaactctt catcttcgat gatcaaggtc aagtcttctt cgatgccact
     1201 tgtgatgtcg ccgtcatcat ctgtgatatc cacagcatca acatcatcca cttcatcctc
     1261 atccacatcg atttcttcat cttcatcagc aacatcgatg tcttccggat catcatcgtt
     1321 gtagtcaatc acatcgtcat catcttctga tgtagcgaag gcgttcaact tcttgcgttt
     1381 cttgcggcgg actttcactt cgtcctcatc gatgctggaa agaatctctt catcaattga
     1441 atcaatcgga taccacgaac gcaatcccca acgattttct cctaaagaaa taaagctgcc
     1501 atcaatgttc aaatccgtgt agaaacgtgt catcttgcct tccaattctt cttccgacat
     1561 ttcaagatag tcctgtatct gaaccaataa ttggttaaag tccagtacgt caccgttttc
     1621 ttcgaaaatt gcgtgtgcaa cttctatcat tgacaattcg tttttgttcg tgccatctaa
     1681 ttgcttcaat ttcaatggtg tcacgtcctt tcaaaagttt actcttaatc atacaatact
     1741 caggtagtaa attgcaattc aattcgataa tctcccaatt tttgttcatt catttggagt
     1801 tcataatgca aggatacttt cccggcaaac ggtttttcct tgaactccag cagcatttta
     1861 tgggtgatcg tctcgatttc catcagtccg gccggcgtcg gaaccatagc catgcctttt
     1921 ccttctgcat cgaaggtcat acggcttgtc tgctccattc tgcggatgat cgtcattttc
     1981 ccttcgcggg acagtttgaa tgtgaccggg atagccgttt cggcttcgat atagcgcaga
     2041 tagagccaat cacccatttc cacgatttgg cccatccctt catataggaa gctttcctgt
     2101 tccccttctt gcttcaccaa cgtttccaat tggaactcga tgggcattcc tttttttaaa
     2161 gacacgcttt tccacctcat tgaccatcgt gcggaagctt cgcggccgca cgtgtttttc
     2221 aagtacagta tagcggtaca aataagcgct ggtcaatcat ttacgggctg attttccaaa
     2281 atgcgaagaa atcctgcctt ccgctgcaat tcacactcaa atcttgcctg aactttgcta
     2341 taatgggagc agaaacaacc caaaagataa ggaagcgcct atgacaaact atttaaaaaa
     2401 caacatttat catatctacg atacttcaaa tacagcattc ggcgccgatc ctctcgcgca
     2461 cttcgcgatg ggcgatgcgt tgatccgctt ggtgggaaac gctgaagctc cggctgcctt
     2521 gcatttttgg ccgatggaaa agctggtcat cctgggcatg atggacacgc gcctgcccca
     2581 tctggaagac ggcctgcgct acttgaggaa cgttccgacg gatatcgtcg tccgcaatgc
     2641 cggcggattg gcagtcgtag ccgatgaggg tatcctgaac ttctccctgg tgcttccgga
     2701 aaccgaaaag gccaaactca ccatccatga cggttatgtg ctgatgaaag aattggttga
     2761 gcaggctttt tctgattccg aacaggtgat tgaagccttc gaaatcagca actcctattg
     2821 tccgggtgac ttcgacctga gcatcaacgg caagaaattc gccggcatcg cgcagcgccg
     2881 tttcaaaaac ggtgtggcca tcatgatcta catgagcgta aacggcaagc aagagcagcg
     2941 cggagaaatg atccgccggt tctacgaaga aagtctgcaa ggcgaagaga cccgctggca
     3001 tttcccggcc gtcgatcccg gatccatggc gaacttggcg gaattgctgg acaaaccgtt
     3061 gaccgtagcc gatgtgaaag cacggatcct gtccgtcttc acgaagaacg gcaacacgct
     3121 cctgcccgga gcattcaacc agcgtttgat ggaagagtat gaaaaagcct atacaaaaat
     3181 gaagaaacgc aatgaaaaaa tcaatcagga ccactatccg gaagcggact gacagaaaga
     3241 ggcgatggga tttgagcaca cactataccg cggaattatt gccgaaagaa aaagtattcc
     3301 gggacccggt ccatgattac atccacatcc agtaccgcat catcatggac ctgatcaatg
     3361 cacccgaatt ccagcgtttg cgtcggatca agcaactggg gacttcttcc tatacttttc
     3421 acggcgccga gcactcccgc ttcaaccatt cattgggcgt ctatgaaatc gcccgccgga
     3481 tttgcgataa gttcgtccgg aactatccca gcaaagcgca tggggatggc ctttgggacg
     3541 atggtgagcg gctcgtgacg ctgtgcgcag ctttgttgca tgacatcggc catggcccgt
     3601 tctcgcatac cttcgaaaag atattcgata cagaccatga agccatcaca atcgagatca
     3661 tcacctcacc ggataccgaa atcaatcgga ttctccaacg ggtcggtccg gatttcccga
     3721 atcaggttgc cgctgtcatc gccaagacct acgacaatcc gcaggtggtg caactgatct
     3781 ccagccagat cgatgccgac aggatggatt acttgctgcg cgacgcctat tattcgggcg
     3841 tcacttacgg caattttgac ctgacgagga ttttgcgcgt catccgtcct tatgagcatg
     3901 gtatcgcctt tgatatttcc ggcatgcatg cggtggagga ttatatcgtc agccgctatc
     3961 agatgtatat gcagatttac tttcatccgg tttcccgcgg catggaggtc gttctcagcc
     4021 atctgttgaa acgggcgaag gatgtctatt tggatgaaca ggccgaattc cggcataccg
     4081 ccactttcct gacgccgttc ttcaaacaga cttggacact gaaggattac ttgcgtttgg
     4141 atgatggcgt cttgacgacc tacttcaacc attggcttga cgaaaaggat ccgattctga
     4201 acgacttggc ctatcgcttc gtcaatcgtc atcctttcat atcggtccgc tacacaaaag
     4261 gacgcgatga tgctacactg gaacgcatga gcgccatcat tgagcgcatg ggcttcaatc
     4321 cggattacta tacggctgtc aacaacagct atgacctgcc ttatgatctt taccgaccgc
     4381 atcaggccac aaccaagacc cagattgaac ttgtccagaa aaacggcagt ttcctggagc
     4441 tttcgcaggc aagttcgatc gtttcggctt tgacgggcaa ggtccgcggg gatgaacgtt
     4501 tctacttccc gcgcgaaatt acccacggcg acgaggaagc tggcatcaat cttttcgagt
     4561 cgctatcgaa ggagttttca actttttttg agaatgatga aattttacca acccaacaaa
     4621 caggaggtac actatgagca tcaaattaat cgccatcgac atcgatggca cattggtcga
     4681 caaggagttc aaaatcaccc cggaagtaaa agcagccatt acggaagcgc gcgaacaagg
     4741 cgtaaaaatc gtactttgca ccggcagacc acttccgggc gtcaaacgct acatcaaaga
     4801 attggaattg gatcaggacg aagattacgt catcacttac aacggctctt tggtgctgtc
     4861 caccgcaaca aacgaagtac tcgtttcaca tacactggat tacagcgact tcgtccggat
     4921 caacgaattg gccgataagt tccatgtgca cacgcacgga atcgacagcg aagccatcta
     4981 taccgcgaac aaagacatca gcatcttcac aacacgcgag tccttcatta cgacgatgcc
     5041 gattcgttat cgctcattgg ctgaattgcc tgaagataaa ctgttcacaa aaatcatgtt
     5101 catcgaccag cccgaactgt tggatatcct gattccggca attccagcgc agttccatga
     5161 ggattatgtg attgtccgca gcgaaccgca tttcctggaa gtgctgaaca aagatgccgg
     5221 taaagccagc gccttggccg aattggctgc gttgctgaat attgattccg aaaatatcat
     5281 ggccatcggc gataacgaga acgacctgag catgatcgaa tacgcaggcg tcggcgtcgc
     5341 gatgggcaac gcagtcgata aagtgaagga ag
//
LOCUS       DAOMUJ010000067         5338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4238_length_5338_cov_2.603824, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000067 DAOMUJ010000000
VERSION     DAOMUJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_5338_cov_2.603824"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1364
                     /locus_tag="PLT73_02805"
     CDS             324..1364
                     /locus_tag="PLT73_02805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRM19343.1"
                     /translation="MVLARARAVDGPDKPFRFAEIKRRELDEQDVLIEIKFAGICHSD
                     IHTAHGEWGHVHYPLVPGHEIAGLVTAVGPGVTKYKIGDRVGVGCMVDSCGECENCLR
                     GEEQYCMKGNVPTYAGVDKYGEPTQGGYSTHIVVTEDFVVRIPDNIGLDAAAPLLCAG
                     ITTYSPLNRWQAGPGKKVAVIGLGGLGHMGVKIAHAMGAEVTVLSQTLSKKEDGLKFG
                     AQHYYATKDPETFKELAGSFDLIINTVSAVINLDAYLGLLKLDGTLVNVGAPGEPLSV
                     NVMSLIGHRRSFAGSMIGGIRETQEMLDFCAEHSIVPNIEVISADQIDEAYERVLASD
                     VRYRFVIDTSTI"
     gene            1563..2180
                     /locus_tag="PLT73_02810"
     CDS             1563..2180
                     /locus_tag="PLT73_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRM19344.1"
                     /translation="MEFISIVEIIGTIAFAMSGALTAIEKDLDYYGIGIFAITTSVGG
                     GIVRDLLIDRPLPASLENPLYALISLLSAGFVILFYTHINKLAKKLQFFDAIGLGAFT
                     AIGAEVAVRLGFQQWYVVVTLAVLTGTGGGLIRDVFAREIPYIFRKEVYAMASILGAI
                     LYIIVFRLAGSQMALYSCFLATTLIRLYCMEKDVHLLKVGKVTLE"
     gene            2454..3617
                     /locus_tag="PLT73_02815"
     CDS             2454..3617
                     /locus_tag="PLT73_02815"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004048395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRM19345.1"
                     /translation="MELTGLKQKFEELFGKKDYTAFFAPGRINLIGEHTDYNGGNVFP
                     CAITLGTYAIAAKNDLKQVRLYSENFPEAGVISFDLADLDHKKADSWANYPKGMLRYL
                     KEAGHAIPEGMDMVIFGNIPNGSGLSSSASLELLMGVVLDNLFDLNVDRLDLIKTGKR
                     VENEFIGVNSGIMDQFAVGMGEENKAILLDCNTLEYEMVPVELGNHAIVIMNTKKRRE
                     LVDSKYNERRSECEEALAKLQKVVSVGSLGELDEETFENAKAVLESDVLYRRARHAVT
                     ENQRTLKAKAALQAGELEAFSQLMNQSHISLRDDYEVTGIELDTLVQAAWDQPGVLGA
                     RMTGAGFGGCAIAIVEKDAIPTFIENVGKTYEAAIGYPAEFYIAEISDGAKRL"
     gene            3717..>4560
                     /locus_tag="PLT73_02820"
     CDS             3717..>4560
                     /locus_tag="PLT73_02820"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252477.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM19346.1"
                     /translation="MDRIAQRNLLLALIGKEDLDTKLEPSQILPDRLDLLDRLVDWAV
                     ANGKIADYQYARETLEAQIMSLITPMPSVINKHFWAVYAENPEAATDYFYELSQNNDY
                     IKTRSIAKNIAFQHRTDYGELEITINLSKPEKDPKQIALAKNVPASAYPACQLCMENE
                     GYAGRIDHPARANHRIVRMPLNGEKWGLQYSPYAYYEEHCIFLAEEHKPMKLDKHTFE
                     KLLGIITQFPHYFVGSNADLPIVGGSILSHDHYQGGRHTFAMAEAPVEQPFEMDRFAS
                     VQAGI"
     assembly_gap    4561..4570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4571..5234
                     /locus_tag="PLT73_02825"
     CDS             <4571..5234
                     /locus_tag="PLT73_02825"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660376.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM19347.1"
                     /translation="GGHYTFAMEKAPVIQEFTVKGFEDVTAGIVKWPMSVIRLAGKDV
                     HELAEAASFILDAWRGYSDEAAGILHVTEGIPHNTITPIARMKDGLFELDLVLRNNRT
                     SPEFPDGIFHPHPDVQNIKKENIGLIEVMGLAILPPRLKDELSEIEKYLNGETAEIAA
                     YHQEWVAGLVDKKARTGADAAEVVQDAVSEAFLRVLEDAGVFKRTPEGQQAFGRFVEQ
                     LR"
     gene            5263..>5338
                     /locus_tag="PLT73_02830"
     CDS             5263..>5338
                     /locus_tag="PLT73_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRM19348.1"
                     /translation="MSVLVTGGAGYIGSHTVVELLKAGQ"
BASE COUNT         1409 a         1179 c         1470 g         1270 t
ORIGIN      
        1 gattgaggat tgagtgactt cggtttgggt agtttcgttt gattcattcg aacaggccat
       61 aagcaataat gcaaccggaa gagcaaaata ggctttcgtt ttcaagttga ttcctcctat
      121 tttcgaattg tttcaatatt aagtataaac tagttatact gagaatagaa ataatgttaa
      181 taataaggaa caaaaaaagc gggagtaatt ttagcgcttc ttagcggtta tcgcttgact
      241 taaccttaac tttaaggagt atgctctttc tacaagagcg agaatgctgc ttttgaatta
      301 aaagcataat ggagggaaaa tagatggtac tagcgagagc aagagcagtc gacggtccgg
      361 acaaaccgtt ccgatttgct gaaatcaaga gacgggaatt ggatgaacag gacgttctga
      421 tcgaaatcaa gtttgcgggt atctgccatt cggatatcca tacagcccat ggtgaatggg
      481 gacatgtgca ctatccgctc gtgcctgggc atgaaatcgc gggattggtc accgcagtcg
      541 gtcctggcgt aacgaaatat aaaattgggg atcgcgttgg tgtcggctgc atggttgatt
      601 cctgcggaga atgcgaaaat tgtttgcgcg gggaagagca gtactgcatg aaaggcaatg
      661 tcccgactta tgccggagtc gataaatacg gcgaaccgac acagggcggc tactcgacgc
      721 atatcgtcgt gaccgaagat tttgttgtgc gcattccgga caacatcggt ctggacgccg
      781 cagcgccgct gttgtgcgcc ggcatcacaa cctactcacc attgaaccgt tggcaggccg
      841 gccccggcaa gaaagtcgcc gtgatcggac tgggcggatt ggggcacatg ggcgtcaaaa
      901 tcgcgcatgc gatgggggct gaagtcaccg tcctttcgca aacgttgagc aaaaaagaag
      961 acggactgaa gttcggggcg cagcattatt acgccaccaa ggatccggaa acattcaagg
     1021 aattggccgg cagctttgat ttgatcatca atacggtgag tgccgtgatc aatctggacg
     1081 cttacctggg cttgttgaaa ctcgacggca cgctggtgaa cgtcggggct ccaggagaac
     1141 cgctgtcagt gaatgtgatg tccttgatcg ggcatcgccg ttcctttgca ggttccatga
     1201 tcggcggcat ccgcgaaacc caggaaatgc tggacttctg cgccgaacac agcatcgttc
     1261 cgaatatcga agtcatttca gccgaccaga tcgacgaggc ctatgaacgc gtcttggctt
     1321 ccgacgtcag ataccgtttc gtgatcgaca ccagcacaat ctgatgaaaa attagttctc
     1381 tgatgttcaa tatttttggg cgcaagggta tatggaaact acagaataaa taggtttgtg
     1441 caaacaggct aaaaaagaat gaaaaagaat gcaaagttta tggcgacttt gcattctttt
     1501 gcgttatact ttttgcaaaa cctaatattt tcagagaact atcatcaggg gggacacatt
     1561 gaatggaatt catttctata gttgaaataa tcggaacgat cgcctttgcg atgtcagggg
     1621 cattgaccgc gatcgagaag gatcttgatt attacgggat cgggatattt gcgatcacga
     1681 cttcggtagg cggcgggatc gtcagggatc tgctgattga tcgccctttg ccggcatcat
     1741 tggaaaaccc gctctatgca ctcatcagct tgctttccgc cggctttgtc attcttttct
     1801 atacgcacat caacaaattg gcgaagaaac tgcaattttt tgatgcgatc ggtcttgggg
     1861 catttactgc tatcggagcg gaggtagctg tgcggctggg ctttcaacag tggtacgtgg
     1921 tggtgacgtt ggcggtattg accggaaccg gtggcggact tatcagggat gtgttcgctc
     1981 gggagattcc ttatatattc cgcaaagagg tgtatgcgat ggcgtccatc cttggggcca
     2041 tcctgtacat tatcgtgttt cgcttagccg gaagtcaaat ggcgttatac agctgttttt
     2101 tggcaaccac tttgatcagg ctctactgca tggagaagga cgttcatctg ctgaaagtcg
     2161 ggaaggtcac acttgaatag tcatccgttc caaagctgcg tgcattattc gatacaatta
     2221 cccaaatatt ttttttacct ggaaataagc taattgaatg ttaaaccgca atgaagggct
     2281 tgacaatgcc taacattgcg gttttttgtg ttaaatgaat caaaatccgc acggggtgaa
     2341 agcgcggcaa aatagcttga cacttttttg ttgtggggtt ataattcatt tagttaatat
     2401 ttagtaaaaa taacaagtaa aaatttatca aaaacaagaa ggagcggatg aagatggaat
     2461 tgacagggtt gaagcaaaaa tttgaggaat tattcggcaa aaaggattac acagcatttt
     2521 tcgcaccagg acggatcaat ctgatcggcg agcacacgga ttacaacggc gggaatgtct
     2581 ttccttgcgc catcacattg gggacatacg ctatcgcggc aaaaaatgat ctgaagcaag
     2641 tccgtcttta ctcggaaaac ttcccggaag ccggcgtcat cagcttcgat ttggccgatc
     2701 ttgatcacaa aaaagctgac agctgggcga actacccgaa aggcatgctg cgctatctga
     2761 aggaagccgg tcatgccatc ccggaaggca tggatatggt cattttcgga aatatcccga
     2821 acggctctgg tctgtcctca tccgcatccc tcgagctatt gatgggtgtc gttctggata
     2881 atctgttcga tttgaacgtg gaccgcttgg acctcatcaa aacaggcaaa cgcgtggaga
     2941 acgagttcat cggcgtaaac agcggcatca tggatcagtt cgctgtcggc atgggcgagg
     3001 aaaacaaagc aattttgctg gactgcaata cgcttgaata tgaaatggtt cctgtagaat
     3061 tgggcaatca cgcaatcgtt atcatgaata caaaaaaacg tcgcgaattg gtcgactcca
     3121 agtacaacga acgccgcagc gaatgcgaag aagccttggc gaaactgcag aaggtcgtat
     3181 cggtaggcag cctaggcgaa ctcgatgaag aaacgttcga aaatgcgaaa gcagttctgg
     3241 agagcgatgt cttgtaccgc cgtgcgcgcc atgccgtaac cgagaaccaa cgcaccttga
     3301 aagccaaagc ggctttgcag gcaggcgaat tggaggcatt cagccaattg atgaaccagt
     3361 cgcatatttc tttacgggat gattatgaag tgaccggcat cgaactggat accttggttc
     3421 aagctgcatg ggatcaaccg ggtgtcttgg gagcgcgcat gaccggagcg ggattcggtg
     3481 gctgcgcaat cgccatcgtc gaaaaagatg cgattccgac ctttatcgaa aatgtcggta
     3541 aaacctatga agcagcgatc ggttatccgg cagaattcta catcgccgaa atcagcgacg
     3601 gcgcaaaacg gttataagga gcggttggta tgacgataaa tgagacagcg tacatgatcg
     3661 atcaagctgt tgtcgatttc gtggaaacgg gcatcgcaaa cggcgaaacg gaagcgatgg
     3721 acaggattgc gcagcgcaat ctgctgttgg ccctgatcgg caaggaagat ttggacacaa
     3781 aattggagcc ttcgcaaatt ttgccggatc gcttggatct gctggatcgc ttggtggatt
     3841 gggcggtcgc aaacggtaaa atcgcggact accaatacgc gagggagacg ctggaggcgc
     3901 agatcatgtc tttgatcacg ccgatgcctt ccgtcatcaa taagcatttc tgggcagtct
     3961 atgcagaaaa tccggaagcg gcaactgact atttttacga gctcagccaa aacaatgatt
     4021 acatcaaaac ccgcagcatc gccaaaaata tcgcgtttca gcaccgtact gattacgggg
     4081 agctggagat tacgataaac ttgtccaaac cggagaagga tccgaagcag atcgctttgg
     4141 cgaaaaacgt acccgcatca gcttatccgg cttgccaact ctgcatggaa aacgaaggct
     4201 atgccgggcg catcgaccat ccggcccgcg ccaaccaccg catcgtccgc atgccgttga
     4261 acggtgaaaa gtgggggctg cagtattcgc cctatgctta ctatgaggag cactgcatct
     4321 ttttggccga ggagcacaag ccgatgaaat tggataagca tactttcgag aagttgcttg
     4381 ggatcatcac gcaattcccg cactatttcg tcggatcgaa tgcggacctg ccgatcgtcg
     4441 gtggatcgat tctatcgcac gatcattatc agggcggacg gcacaccttc gcgatggcgg
     4501 aagcgccggt tgaacagcca tttgaaatgg atcgattcgc ttcggttcag gctggcatcg
     4561 nnnnnnnnnn ggggggacat tatacctttg ccatggaaaa agcgcctgtc atccaggagt
     4621 ttactgtcaa gggctttgaa gatgtcacag caggcatcgt aaaatggccg atgtccgtca
     4681 tccggcttgc cggtaaggac gtgcatgaat tggcggaggc ggcatccttt attctggatg
     4741 catggcgggg ctattcggat gaagcggccg gcatcctgca tgtcaccgag ggcattccgc
     4801 acaacacgat cacgccgatt gcgcgcatga aggacggatt gttcgaactg gatctggtgc
     4861 tgcgcaacaa ccggacttcg ccggagttcc cggacggtat tttccatccg catccggacg
     4921 tgcagaacat caagaaagag aatatcggcc tgatcgaagt gatggggctg gcgattttgc
     4981 cgccgcgctt gaaggatgaa ttgagcgaaa tcgaaaagta tctcaatgga gaaacagcag
     5041 aaatcgctgc ttaccatcag gagtgggttg caggcttggt ggacaaaaag gcgaggaccg
     5101 gtgccgatgc ggcagaagtg gtgcaggatg cggtaagcga agcctttttg cgggtgctgg
     5161 aggatgccgg tgtgttcaaa cgtacccccg aaggccagca agcgtttggc cgtttcgtcg
     5221 aacagttgcg ttgatttttg aaaggaaagt ggaggggata aaatgtcagt attagtgact
     5281 ggaggcgccg gctacatcgg cagccatacc gtagtggaat tattgaaagc aggtcagg
//
LOCUS       DAOMUJ010000068         5337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4241_length_5337_cov_2.661113, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000068 DAOMUJ010000000
VERSION     DAOMUJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4241_length_5337_cov_2.661113"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLT73_02835"
     CDS             <1..837
                     /locus_tag="PLT73_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM19349.1"
                     /translation="EPYVIPEACPACASHLVHLEDEVALRCINPKCPAQIKEGLSHFV
                     SRNAMNISGLGVRVVSQMFEKGLVKDVADLYKLTEEDLYQLDKIKEKSANNIVTAIDQ
                     SRGNSFERLLFGLGIRHVGAKAAASLAAEFETIDRLMQATKEEIMAVEGMGDIIADSV
                     VAYFQLPEVKDLIEEFRANGVNLTYLGKKKAEVAAVDSIWNGKTVVLTGKLTHYNRTE
                     ATEMIEALGGKVTGSVSKKTDWIVAGEEAGSKLAKAQSLGIPVWTEEDMVKNLERSDS
                     SE"
     gene            830..1963
                     /locus_tag="PLT73_02840"
     CDS             830..1963
                     /locus_tag="PLT73_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HRM19350.1"
                     /translation="MNKAKSVILMTVSGAAMFLLASCGQLSETQTTENTTNTGPEKVT
                     VQTTQNQLSTDYYPALIVDGKYQFSQNRGVSLSLNSTANIKDFEADLLDVAKNVFPTD
                     QYFFQEGQVIDYDTTRLWLGRYSDSNPDGLNPTDNGSTDAATREPIYLEQILEQDYMI
                     QNENGFELAGMAIGLAMNSVDYYKVNDVPMEQAISRDKLEEQAKAYANTIITRLRQTE
                     GLESIPIVIGIYEQSAQDSPVGGSYLFEGVSTEGSAIGEWITRNESKVVFPLQSGTQT
                     EESSNFDNFKNEVQDFFPNLNGIVGEGKYVDGQLMSLEIDITTQFYGETEIIAFTQHV
                     TDAAGRFLPQNIPVEITIESINGIESFLTRENDSQGFSYHIFD"
     gene            2092..2397
                     /gene="gatC"
                     /locus_tag="PLT73_02845"
     CDS             2092..2397
                     /gene="gatC"
                     /locus_tag="PLT73_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRM19351.1"
                     /translation="MAITEEQVRHVAKLAKLEFAPHEIKHFTEQLGDIIDMVEQLEAV
                     DTTDVPVTSHGYALKNVMREDVAEPGMDRDLLFKNVKTAEDGMIQVPAILDNEVEGA"
     gene            2394..3848
                     /gene="gatA"
                     /locus_tag="PLT73_02850"
     CDS             2394..3848
                     /gene="gatA"
                     /locus_tag="PLT73_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRM19352.1"
                     /translation="MSIFNETLTSLHEGLVNKEFSSVELTQAAFKRIAETDGKVEAFL
                     ALNEEGALKQAQAADEKGYADGNVLNGIPLGIKDNIVTEGVTTTAASRMLGDFMPIYD
                     ATVVTKLKEAGAVNVGKLNMDEFAMGGSTENSYYKKTKNAWDLTKVPGGSSGGSAAAV
                     ASGEVVASLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRYGLIAFASSLDQIGPMTRNV
                     TDNALLLEAISGYDKRDSTSLNMEVPKFSANLNGKVAGMKIALPKEYFQDGVSAEIQE
                     AVRKAAAQFEEMGATVEEVSMPTLAYGIPAYYIIASSEASSNLQRFDGVRYGYRAENV
                     NSLEELYIKSRSEGFGMEVKRRIMLGSFSLSAGFYDAYFKKAGQVRTLIKRDFANIFA
                     GYDLILGPTTTTTAFGIGEKNDDPLAMYMDDLLTVTINLAGVPAISVPGGFAADGLPI
                     GIQLIGNYFEEAKIYQAAFALEQANDYLDQHPNL"
     gene            3861..>4802
                     /gene="gatB"
                     /locus_tag="PLT73_02855"
     CDS             3861..>4802
                     /gene="gatB"
                     /locus_tag="PLT73_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRM19353.1"
                     /translation="MNYETVIGLEVHVELKTESKMFSPSPAHFGAEPNTNTNVIDWGY
                     PGVLPVINKGAVEFCMKAALALNCEISTETHFDRKNYFYPDNPKAYQISQLDNPIGHD
                     GWVEIEVEGVKKKIRIERVHLEEDAGKNMHGSDGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLDAVRQIIMYTDVSDVKMEEGSMRCDANISIRPFGQEKFGTKTELKNLNSF
                     NFVRRGLAHEEKRQAQVLNAGGVIQQETRRFDETNGETILMRVKEGESDYRYFPEPDL
                     PGLTISQEWIDRVKASIPEMPAKRRARYISEYELPEYD"
     assembly_gap    4803..4812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4813..5287
                     /locus_tag="PLT73_02860"
     CDS             <4813..5287
                     /locus_tag="PLT73_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRM19354.1"
                     /translation="LSKEMSDFFEGAIAAGADAKLASNWLMGEVSAYLNSEKVELADT
                     KLTPANLAGMITLIEDGTISTKIAKKVFRLLATKGGDAKSVVEAEGLIQMSDPAQLLP
                     IINAVLDNSQQSVDDFKAGKDRAKGFLVGQIMKQTKGQANPGMVNQLLAQELEKR"
BASE COUNT         1584 a         1292 c         1347 g         1104 t
ORIGIN      
        1 gaaccctatg tcattccgga agcgtgccct gcctgcgcta gccacttggt gcatctggag
       61 gacgaagtag ctctgcgctg catcaatccg aagtgccctg cccagatcaa agagggcctg
      121 tcccacttcg tttcgcgcaa tgccatgaac atcagcggtc tgggcgttcg cgtcgtcagc
      181 cagatgttcg aaaaagggct cgtcaaggat gtagccgacc tgtataaact gacggaagaa
      241 gacctttacc aattggataa aatcaaagaa aagtccgcca acaatattgt gacggccatc
      301 gatcagagcc gcgggaactc ttttgaacgc ctgctgttcg gcttgggcat ccgtcatgtc
      361 ggagccaaag cggcagccag cctggcagcg gaattcgaaa ccatcgatcg gctgatgcag
      421 gcgacaaaag aagaaatcat ggccgtcgaa ggcatgggtg atatcatcgc ggatagtgtt
      481 gtggcctatt tccaactgcc tgaagtgaag gacctgattg aagaattccg agcgaacggc
      541 gtgaatctga cctatctcgg caagaaaaag gctgaggtgg ctgctgtcga ttccatctgg
      601 aacggcaaaa cggtcgtatt gacgggcaaa ctgacgcact ataaccgaac cgaagcgacg
      661 gaaatgatcg aggctctcgg tggaaaagtg accggcagtg tctccaaaaa gaccgactgg
      721 atcgtggccg gagaagaagc aggaagcaaa ttggcaaaag cccaaagtct gggcatcccc
      781 gtctggacag aagaagacat ggtgaaaaac ttggaaagaa gtgacagcag tgaataaagc
      841 gaaaagcgtc atccttatga ctgtctccgg agcagcgatg ttcctgttgg ccagttgcgg
      901 acagctatca gagacgcaaa cgacggagaa cacaaccaat accggaccag aaaaagtgac
      961 tgtccaaacg acacaaaatc agttatcgac cgattattat ccggccttga tcgtggacgg
     1021 caaataccaa ttcagccaga atcgcggcgt cagcctctcg ctgaactcga cagccaacat
     1081 caaagacttt gaggcggatc tgttggatgt agcgaaaaat gttttcccga ctgaccaata
     1141 cttttttcaa gaaggccaag tcatcgatta tgatacgaca aggttatggt tggggcgtta
     1201 cagcgactcc aatccggacg gtttgaatcc gaccgataac ggcagcaccg atgcggccac
     1261 cagagagccg atctatctgg agcaaatttt ggaacaggat tatatgatcc agaatgaaaa
     1321 tggctttgag ttggccggaa tggccatcgg attggcgatg aattcggtcg actattacaa
     1381 agtgaatgat gtgccgatgg aacaagctat ttcccgcgat aaactggaag aacaggcaaa
     1441 agcttatgca aatacaatca ttacccgttt gcgccagaca gagggattgg aaagcattcc
     1501 gatcgtcatc ggcatatatg agcaatcagc acaggacagc ccggtcggcg gctcttatct
     1561 gttcgaaggc gtctccacgg aagggtctgc gatcggtgaa tggatcaccc gcaatgaaag
     1621 caaagtggtt ttcccgttgc aaagcggaac gcagacggaa gagtcttcca actttgacaa
     1681 cttcaagaat gaagtacagg atttcttccc gaacctgaac ggaatcgtcg gtgaaggcaa
     1741 gtacgtcgat ggacaactga tgtcgctcga aatcgacatc acgactcagt tctatgggga
     1801 aacggaaatc atcgccttca cccaacacgt aaccgatgca gcaggacggt tcctgcccca
     1861 aaacattccc gttgagatca cgattgaatc gatcaacggc atcgaatcgt tcctgacaag
     1921 agaaaacgac agccagggct tcagttacca tatcttcgat taggtaacgg tttccttaat
     1981 aaagggctgg aaacgtgaga aaactcccac aaaccgatga tttttatgct aaaatgaaca
     2041 ggtatgtaaa aaaacgaaac ctacacaaaa cgacagaaag aagggtatta catggcaatc
     2101 actgaagagc aagtgcgtca cgtagcaaaa ctggccaagc tggaattcgc tcctcacgaa
     2161 attaaacatt ttacagaaca attgggcgac atcatcgaca tggtggaaca attggaagca
     2221 gtcgatacaa ccgatgtacc cgtgacttcc catggctatg cattgaagaa tgtcatgcgt
     2281 gaagatgtag cggaaccagg aatggatcgc gacctcttat tcaaaaacgt caagacagcc
     2341 gaagacggca tgatccaagt gcccgcaatt ttagataacg aggtggaagg cgcatgagta
     2401 ttttcaatga aacgctaacg agcctgcatg aaggtttagt caacaaagaa ttttcttccg
     2461 tggaactgac acaagcagcc tttaaacgca tcgctgaaac agacggaaaa gtggaagcat
     2521 tcttggcttt gaacgaagaa ggcgccttga aacaagcgca agcggctgac gaaaaaggct
     2581 acgcagacgg caatgtcctg aacggtattc ctttagggat caaagacaac atcgtcacag
     2641 aaggcgtgac aacgacagcg gcgagccgca tgttgggcga cttcatgccg atctacgacg
     2701 caaccgtcgt cacaaaattg aaggaagccg gcgcagtcaa cgtcggaaaa ctgaacatgg
     2761 acgaatttgc catgggcgga agtacggaaa actcctacta caaaaaaacc aaaaatgctt
     2821 gggatctgac gaaagttccc ggcggttcat ccggtggttc tgctgctgcg gttgccagcg
     2881 gtgaagttgt cgcttccttg ggttctgata caggtggaag tattcgccaa cctgctgctt
     2941 tcaatggtat cgtcggcatg aaaccgacat acggccgcgt atcccgttac ggtttgatcg
     3001 cttttgcgtc cagccttgac caaatcggtc cgatgacgcg caacgtaacc gacaacgcct
     3061 tgttgttgga agcaatcagc ggctatgata agagagactc cacgagctta aatatggaag
     3121 tgccgaaatt cagcgcgaac ctgaacggaa aagttgcggg catgaaaatc gcattgccta
     3181 aagaatattt ccaagatggc gtatccgctg aaatccaaga ggctgtcaga aaagcagctg
     3241 ctcaattcga agaaatgggc gctaccgtag aagaagtcag catgccaact ttggcttacg
     3301 gaatcccggc ttactacatc atcgcttcat cagaagcttc atccaacctg caacgttttg
     3361 acggagtgcg ttacggctac cgtgcagaaa atgtgaattc gttggaagaa ttgtacatca
     3421 agagccgttc cgaaggtttc gggatggaag tgaaacgccg catcatgttg ggatccttct
     3481 cattgagtgc cggtttctat gatgcttact tcaaaaaggc tggtcaggtc cgcacgctga
     3541 tcaaacgcga ttttgcgaac atcttcgcag gctacgatct gattctggga cctaccacta
     3601 caacgactgc tttcggaatc ggcgaaaaga acgacgatcc attggctatg tacatggacg
     3661 acttgttgac cgtaacgatc aacttggctg gcgttccggc catttccgtt cctggcggct
     3721 tcgcggctga cggcttgccg atcggtatcc aattgatcgg taactatttc gaagaagcaa
     3781 aaatctatca agccgcgttt gcgttggaac aagcaaacga ctatctagac caacacccaa
     3841 acctgtagga ggaaaacatc atgaattatg aaacagtcat cggactggaa gtccatgtgg
     3901 aattgaaaac tgaatcaaaa atgttctccc catctccagc acactttggg gcagaaccga
     3961 acacaaacac aaacgtcatc gactggggtt acccgggcgt cttgcctgtc atcaacaagg
     4021 gcgcagtcga attctgcatg aaggccgctt tggctttgaa ctgtgaaatc tctactgaaa
     4081 cgcacttcga ccgcaaaaac tacttctatc cggacaatcc gaaagcgtac caaatttcgc
     4141 aattggataa cccgatcggc catgacggct gggtcgaaat cgaagtggaa ggcgtcaaga
     4201 aaaagatccg catcgaacgt gtccatcttg aagaagatgc cggcaaaaac atgcacggat
     4261 cggacggcta ttcttatgtc gacttgaacc gtcaaggaac accactgatc gaaatcgtat
     4321 cggaagcgga tatgcgctct cctgaagaag cttatgctta tctggatgcc gtacgccaaa
     4381 tcatcatgta cacagacgta tccgacgtga agatggaaga aggctccatg cgttgcgatg
     4441 ccaacatctc catccgccca ttcggccaag aaaaattcgg aacgaagaca gaattgaaga
     4501 acttgaactc cttcaacttc gtccgcagag gcttggctca tgaagaaaaa cgccaagcgc
     4561 aagtattgaa tgctggcggg gtcatccaac aagaaacacg ccgtttcgat gaaacaaatg
     4621 gcgaaacgat cctgatgcgc gtcaaagaag gcgaaagtga ttaccgttac ttcccggaac
     4681 cggacctgcc aggcttaacc atttcccaag aatggatcga ccgcgtaaaa gcaagtattc
     4741 cggaaatgcc agcaaaacgc cgcgcaagat acatttcgga atacgagctg cctgagtacg
     4801 atnnnnnnnn nncctttcga aagaaatgtc tgacttcttc gaaggcgcta tcgctgccgg
     4861 tgccgatgcc aaattggctt ccaactggtt gatgggcgaa gtttccgctt atctgaacag
     4921 cgaaaaagtg gaactggctg acacaaaact gacgccagct aaccttgccg gcatgatcac
     4981 attgatcgaa gacggcacca tcagcacgaa gatcgccaag aaagtcttcc gcctgttggc
     5041 aacaaaaggc ggcgacgcca aatccgtcgt tgaagcagaa ggcctgatcc aaatgagcga
     5101 tccagcccaa ttgttgccga tcatcaacgc cgttttggac aacagccaac aatccgttga
     5161 cgacttcaaa gccggaaaag accgcgccaa aggcttcctc gtaggccaaa tcatgaaaca
     5221 aaccaaaggc caagccaacc caggcatggt caaccaacta ctagcgcagg aattagaaaa
     5281 aagataggat acgatgaatt gcgggtaggg ggcgagtact aacgagattt aggaaca
//
LOCUS       DAOMUJ010000069         5325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4254_length_5325_cov_2.666983, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000069 DAOMUJ010000000
VERSION     DAOMUJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_5325_cov_2.666983"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLT73_02865"
     CDS             complement(<1..726)
                     /locus_tag="PLT73_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRM19355.1"
                     /translation="MITVIGITADNLVHTDIDLDATDLSAYKWIWADFNEPSPSEVKY
                     LEKTFHFHPLAIEDCLQILQRPKLDYYDDYTFYVTHHVREEGRDLFKEELDFFVSEKL
                     IVTFQLAHSKEVAYVQKQLLAQHNAENWDPYFIFYRILDQIVDNYFPLIHDIEDRLAQ
                     LEEDNLNKTTAAFIPELMESRHLLLGLLQTVNPMRDLLYRMLNSQHLEGVRKRRSYFS
                     DIHDDLLKVSEMITSNREVTTDMR"
     gene            873..1676
                     /locus_tag="PLT73_02870"
     CDS             873..1676
                     /locus_tag="PLT73_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM19356.1"
                     /translation="MIKLILTDMDGTFLNSQGDFNRELFKQVKQMMEEKGVVFAPCTG
                     KQSERVEEIFGEDAADFWILGDSASRIKRNGTVVYESLLENTTGLAILRELEEMQLGH
                     TLIACTKEAAMIKTDLSPEESAAVRRSYKKVVTVADFGSIEDDFIKITVRDMQERCIE
                     TAEKLAAFRNRAHIVASEKAWLDITDLNVHKGSTVAHLQELLQVTPEETMVFGDGMND
                     IEMMKSGRYSFAVRNAYPIIKDTAAFTTGRTNDEDAVSHTILQLLALQG"
     gene            1808..2191
                     /locus_tag="PLT73_02875"
     CDS             1808..2191
                     /locus_tag="PLT73_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM19357.1"
                     /translation="MNIKKASEETGVSADTIRYYERIGLIPPIKRNENGVREFDEEDL
                     KWIAFSRQMRNAGLSIESLIEYLALFRSGEETVPARMDLLVEQQHELQERIDVMQQAM
                     ERLTFKIENYTSHMVPSENKLREFK"
     gene            complement(2266..2721)
                     /locus_tag="PLT73_02880"
     CDS             complement(2266..2721)
                     /locus_tag="PLT73_02880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRM19358.1"
                     /translation="MTETKLSKLRITGQSHNGITTTVKVRNLPEFFVDESERMGGDNK
                     GPNPLEYLLGSLAGCTSIIAYYVSEQQGFSYRGLSFTVEGTFDPRGFAGEDDIRTYFQ
                     EVTLVNHIETDETDEAIEKLATEVERRCPVYNLMLDAGVDMSTQWIRTN"
     gene            2925..3272
                     /locus_tag="PLT73_02885"
     CDS             2925..3272
                     /locus_tag="PLT73_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19359.1"
                     /translation="MEKKDLDLILANFGPEKEFIGDGYFRIRDKGNEHYEIAYLVSAC
                     CGTTNYFPQITVHVEGDRVVPDSLLDLVSNPAKMLSYSPETSAVMEEELDKLCQKFLD
                     IKGLKTANAGTPD"
     gene            complement(3365..3598)
                     /locus_tag="PLT73_02890"
     CDS             complement(3365..3598)
                     /locus_tag="PLT73_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19360.1"
                     /translation="MDKLPKDNHDSIDATWLMEEPYLDIIPEDPPQETEILYCQCKKP
                     SSVYQESNDLGHWLKCGDCNRVIEGSFEAAKPY"
     gene            complement(3755..4312)
                     /locus_tag="PLT73_02895"
     CDS             complement(3755..4312)
                     /locus_tag="PLT73_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRM19361.1"
                     /translation="MLKEPNAKTTEMDLLENKVDLYLGMLPIHNEQLSYTMLADDKWC
                     VIVPDTSPLYQPGLKEIEKFPYPLNLLKNEKYVLTTSQSGIRKQANEFLKHLDIKADV
                     VMESQNISTAAALARKGMGLTFLPKSALNNSELMDSYNIFYIETSIVRTRYFIAYSKE
                     KTLSSVDLAMIDMAKELMKAYSDSI"
     gene            4347..4583
                     /locus_tag="PLT73_02900"
                     /pseudo
     CDS             4347..4583
                     /locus_tag="PLT73_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163894.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4879..>5325)
                     /locus_tag="PLT73_02905"
     CDS             complement(4879..>5325)
                     /locus_tag="PLT73_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19362.1"
                     /translation="SAFNWSDLHPHTFRSAQPHKDTLAEADRLRDHIQGKDDKIIYTY
                     NMEENREHEIVLQKAFEAKDDVLYPRCVRAENLAPEEDHTRLDITDGTLDLAYKDSGE
                     IAKAINEELAYLNVSGLGEGLPGLDEDEWVALDDDEDEWEDDGGHI"
BASE COUNT         1355 a         1156 c         1358 g         1456 t
ORIGIN      
        1 gcgcatatct gtcgtcacct cgcggtttga agtaatcatc tcggatacct tcagcaaatc
       61 atcgtggata tccgaaaaat aactccgcct tttgcggacg ccttccaggt gttgggaatt
      121 cagcatgcga taaagcagat cccgcattgg attgacggtc tgcagcagac ccagcagaag
      181 atgccgagat tccatcaatt ctggtatgaa tgcggcagtc gttttgttca gattatcctc
      241 ttcaagttga gccaacctgt cttcgatgtc gtggattaaa gggaaataat tatccacaat
      301 ctgatcaagg attctgtaaa aaataaagta cgggtcccag ttttcggcat tatgttgggc
      361 caacaattgt ttctgtacat aggctacttc ttttgagtga gcgagttgaa aagtgacgat
      421 gagtttttca ctgacaaaga agtccaactc ttctttgaag aggtctctac cttcttcgcg
      481 aacgtgatgc gtcacataaa atgtgtaatc gtcataataa tccagcttcg gacgctgcag
      541 tatctgcagg cagtcttcga tagcgagtgg gtggaaatgg aaggtcttct cgagatattt
      601 tacttcggag gggctaggtt cattgaagtc ggcccagatc catttgtagg ctgaaaggtc
      661 ggtcgcatcc aaatcgatgt ctgtgtggac gagattgtca gctgtgatgc cgataactgt
      721 tatcatataa ttgcaccttc tttacgagtg atttcaatat ccattatacg ggaaatgcat
      781 tgccgatgcc atttttgggt cattcagcat tccagtagca gagaaatgtg cgataatggg
      841 agcaagaaga caaacggaat ggaggacatc ggatgatcaa gttgattttg actgatatgg
      901 atggtacttt tttgaacagc caaggggatt tcaacaggga acttttcaag caggtcaagc
      961 agatgatgga ggaaaaagga gtcgttttcg cgccttgtac gggcaagcag agtgagcgtg
     1021 tcgaagaaat attcggtgag gatgctgctg atttttggat tctcggggac agtgcttccc
     1081 ggatcaaacg caacggcaca gtcgtctacg aatcgttgtt ggagaatacg acaggcctgg
     1141 ccatcctgcg cgaattggag gagatgcagc ttgggcatac gttgattgct tgtacgaaag
     1201 aagccgccat gatcaaaact gatctgtcgc ccgaagaatc tgcagctgtc aggagatcct
     1261 acaaaaaagt agttacggtg gcggatttcg ggagcatcga ggatgatttc atcaaaatca
     1321 cggtgcgcga tatgcaagag cgctgcatcg agactgctga aaaactggct gcttttcgca
     1381 atcgggcgca tatcgttgct tcggaaaagg cctggctgga catcaccgat ttgaacgtgc
     1441 ataaagggag cacggtcgcc catctgcagg agctgctgca ggtcaccccg gaagaaacga
     1501 tggtgttcgg agacggaatg aacgacatcg aaatgatgaa gagcggaaga tacagtttcg
     1561 ctgtgcggaa tgcttaccca atcatcaagg acacggcggc cttcaccacg ggcaggacaa
     1621 atgatgaaga tgcggtgtcg cacacgatcc ttcagttgct tgcgctgcaa gggtagcggt
     1681 ttattgaaga gggttagtcc cccgttgccc gaagattcca tttgacttgg agtccactcc
     1741 aaggtgtagg atacaattga agctaactgt caggataagt tttgatgccg aaaggatgga
     1801 taagatgata aacataaaga aagccagcga ggaaacgggt gtatcggccg atacgatccg
     1861 ctactatgag cggatcggtc tgattccgcc gatcaaaaga aacgaaaacg gggtccgtga
     1921 atttgatgaa gaagatctga agtggatcgc cttcagtcgc cagatgcgca atgcgggctt
     1981 atcgatcgaa tctttgatcg aatatctcgc actcttccgc agcggtgaag agactgttcc
     2041 ggcaaggatg gatcttttgg tcgaacaaca gcatgaactg caggaacgga ttgatgtgat
     2101 gcagcaggca atggaacggc tgacattcaa aatcgaaaat tatacaagtc atatggttcc
     2161 gagcgaaaac aaattgaggg aattcaagtg attcaagcct aaaaagctcc cgtcaaaaca
     2221 ttgcagctat acagcgatgt tttgacggga gcttttgcat ttcttttagt tggttcggat
     2281 ccattgggtc gacatgtcga cgcccgcatc cagcatgaga ttatagacgg gacaccttct
     2341 ttcaacttca gtcgccagct tctcgatggc ttcgtcggtt tcatcggtct cgatatggtt
     2401 cacgagcgtg acttcctgga agtaggtccg tatatcatct tctccggcaa aaccgcgcgg
     2461 gtcaaaagtg ccttcgacgg tgaacgacag gcctctgtag gagaaacctt gttgttcgga
     2521 aacataatag gcgatgatcg aggtgcagcc tgccagcgaa cccaaaagat actccagcgg
     2581 attcggacct ttgttgtcgc cgcccatccg ttccgattca tcgacaaaaa attcgggcag
     2641 attgcggact tttaccgtag tggtgatgcc attgtgcgat tgaccagtga tcctcagttt
     2701 cgataatttt gtttctgtca tctggataac cccgtttttg ttttttggta gtgcttactt
     2761 atttatttta tcacagcagc tcggtatagg cttaccagaa gctctgatcc gccccgggca
     2821 ggatatccaa aatagcccgc gagtggtata ctattcctga agagaaagtt gctgacaggc
     2881 aaatgtttgc tcacgtttat tcatgaagaa aaggggtttt ctcaatggaa aaaaaggatt
     2941 tggatcttat attggctaat tttggaccgg aaaaggaatt catcggcgat ggctatttcc
     3001 gtatccgcga caagggcaat gagcattatg agatcgctta tcttgtatcc gcctgttgtg
     3061 gaacaacgaa ttacttccca cagattacgg ttcatgtcga aggcgataga gtggttcccg
     3121 actccttact ggatctggta tccaatccgg caaaaatgct cagttattca cctgagacga
     3181 gtgctgtgat ggaagaggaa ttggataaac tctgccaaaa gtttttggat atcaaagggt
     3241 tgaaaacagc aaacgccgga acaccagact gatctctggt gttttttatt aggatgcaaa
     3301 aaggaacaaa caaagtgcga cactgtgcac ttgtttgctc ctttttgttt tttttacgat
     3361 tggattaata aggctttgcc gcttcaaacg agccttcgat gacgcggttg cagtctccgc
     3421 acttcagcca gtggccaaga tcgttgcttt cctgataaac actcgagggc ttcttgcatt
     3481 ggcagtaaag gatttcggtt tcttgagggg gatcttcggg aatgatatcc agatagggtt
     3541 cttccattaa ccaagtggca tcgatagaat cgtggttatc ttttggtagt ttatccatga
     3601 taatccctcc tatgggtaag ttgtacgttc ttgtacgcaa agtttaagga gcatctttgc
     3661 tcactttgat gatacaactt ctgctgccga aggccaaatg atatggttcc gaatggaata
     3721 agcggagccc attttcttat gcagatgcta attttcaaat ggaatcgctg taggctttca
     3781 tcaattcctt cgccatatcg atcatggcca agtcgactga ggaaagagtc ttttctttgc
     3841 tgtaggcgat gaaataacgg gtgcggacga tgctggtttc gatataaaaa atattgtagc
     3901 tgtccatcaa ttcgctgtta tttagcgcac tcttcggcag aaaagtcagt cccatccctt
     3961 ttctcgcaag cgctgctgcg gttgaaatgt tctgcgattc catgacgaca tccgctttga
     4021 tgtccagatg cttcaggaat tcgtttgcct gtttgcggat cccggattgg gaagtggtca
     4081 gcacatactt ttcgttcttc aacaggttca aaggataagg gaatttttcg atttccttca
     4141 agccgggctg atacagcggg ctcgtgtccg gaacgatgac gcaccatttg tcatccgcaa
     4201 gcatagtgta ggaaagctgt tcattatgga tcggcagcat gcctaagtag aggtcgactt
     4261 tgttttccag caggtccatt tccgtcgttt tggcgttggg ttctttgagc aaagctgtat
     4321 tcgaaggcga tgtcgagaac ggatcggtca tggataactt cggaccgaaa ttccaaggcg
     4381 tcaactacgg tatcgtgttc atcggttact cgacagccgc ttttgttgca cctaaagtaa
     4441 cagcgggaat cgctgctgcg aacaacggtg acttcactaa agctttttat gtcgctatcg
     4501 tggttgcggt tgcaggtttg atcctggatc ttgtctacaa gaaaaaaaca gcaacgaaag
     4561 caatcacaga aacagctcaa taaacaaaaa caccttctcg aacgcgagaa ggtgttttta
     4621 ttgaaatgat ttgtctgata accgggagtt atccgacatt gcgtgagaat gttccacaga
     4681 agttgtcgga ttcttccggg ttatccgaca actaggcagt ccccggtgtt gattatgtcg
     4741 gattcttccg agttatcgga caacccgaca gccctcggga aggattatgt cgaataatta
     4801 cggattatcc aacaaataga gactggtcac gcggttgatt ttgattactt gcagatattt
     4861 ccttttgcaa caatgccttc aaatgtggcc gccgtcgtct tcccattcat cttcgtcatc
     4921 atccagagct acccattcat cttcatcgag ccccggcagg ccttcgccaa ggccgctgac
     4981 gttcagatag gccaattctt cattgatcgc tttcgcgatt tccccggaat ctttgtatgc
     5041 gaggtcgagt gtgccgtccg tgatgtccag gcgggtatgg tcttcctcag gcgccagatt
     5101 ctcggcgcgc acgcaacggg gatagagcac gtcatccttc gcctcgaagg ctttttgcag
     5161 gacgatttca tgttcccggt tctcttccat gttgtacgtg taaatgattt tatcgtcttt
     5221 tccttggatg tggtcccgca gacgatcggc ttctgctaat gtatccttat gcggctgggc
     5281 agaccggaaa gtgtgcgggt ggagatcgga ccaattgaat gcact
//
LOCUS       DAOMUJ010000070         5324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4255_length_5324_cov_2.918011, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000070 DAOMUJ010000000
VERSION     DAOMUJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_5324_cov_2.918011"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="PLT73_02910"
     CDS             complement(<1..2081)
                     /locus_tag="PLT73_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19363.1"
                     /translation="MKKKALWKDIWIEIGKSKARFLALLAIITLGVAFFAGIKAAGPD
                     MLDTANQYYEDNNLYDLKVLSTYGLEEADIAILDETADLSVHPMRTVDIEMEGSDFLV
                     KVFPLQSGADPVNAYALVDGRLPETSGEVALDAKQNLSSTYQIGDTITFRHESDEDLS
                     DEEKENRISLKEQEYTVVGFVDSPMYIETIMRGSTNVGKGSLDAFVVVPESDILGSIY
                     TEAYLTMAPTVAETGYTDAYDEQVDRLADEVELALNGRPLERINEIRAEGQRKIRDAE
                     DELQEGKDKLAEAEQELQDARQQLDDGTALYEENRQLFLDEIAQAESTLTASQAEIDA
                     GMAEYRAGLATYLENKALYDEAKVAWEAQKKALQGQNDSDASLDALLASLPDTPEGRE
                     IAELAQMLLDGQAEVEATQSSLEMKALELQERAAALASKGELLLAEQMSLEADAAAYQ
                     KELAIFTADMTAFQTEKAIKQAEFSTRQAAIDSERAALEELDPETDPSVAERLTALDE
                     EQAALDAERAALAQAETELTVRQSELEQEGASLSTRQEALTAAAAAFQAERASEEAAL
                     MEEQRLLESAGNELAARGEELLDQQQLLEEKIAAFMADAEAQIAAADAQFAEQGIALE
                     NGRLELASAFSQLEDGQIQINNGWSELESQRVSGEQALGEAWKEIQQGEAAYQEGLVT
                     FTAERVDAEIE"
     gene            complement(2097..2798)
                     /locus_tag="PLT73_02915"
     CDS             complement(2097..2798)
                     /locus_tag="PLT73_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19364.1"
                     /translation="MAYISLKNVSKFYQMGETRITANDKISFDINEGEFVVILGPSGA
                     GKSTVLNILGGMDDADEGHISIDGVDISQFTSKALTTYRRRDVGFVFQFYNLVPNLTA
                     KENVELASQISPHARDAAEVMREVGLGERMDNFPAQLSGGEQQRVAIARALAKQPKLL
                     LCDEPTGALDYETGKQVLKLLQDTCLDTGTTVIVITHNQAIAAMANRIIEINNAKVRA
                     IHENPNPKPVSEIEW"
     gene            complement(3022..3549)
                     /locus_tag="PLT73_02920"
     CDS             complement(3022..3549)
                     /locus_tag="PLT73_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM19365.1"
                     /translation="MDFMKVMHTRQTTRSFQKFALSQGQVELMLQAGQNAPISRGRFE
                     DYHITVIKNPEILAAITSAAKEVTGSTDKDPLYGAPAFFVVSAKEPAKPGSIASAATI
                     VENMHLQATAELLGSCFLMGIMANGTLDAEGIREKMQIPDGFTPVAGMVAGYPHGYLH
                     DRPRTLEKIAVNIVE"
     gene            3813..5078
                     /locus_tag="PLT73_02925"
     CDS             3813..5078
                     /locus_tag="PLT73_02925"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM19366.1"
                     /translation="MKTRIIIVGGGSSGLMAACTAAEAGAEVTLLEQNPSLGKKLLLT
                     GGGRCNVTNNRPEEEIIRHIPGNGKFLHSSFTQFSQYDIMTFFIERGVALKEEDHGRM
                     FPVTDKARTILEAFIEELKRLQVQIRTNIKVERILIENEQVTGVLLEDGEILPADRII
                     LATGGKALPRTGSKGDGYKFAKAAGHTITELYPTEAPITSDADFIRASELKGLSLRNV
                     ALSIRNKKGKTVVSHQMDMIFTHFGVSGPAALRCSMFVHQTMKRDKTETVTMSLDVLP
                     ELSLGAVEQQLQKLIKAQPEKSVKNGWKDLMPERYLLFGCTQAAIDPQKSLKELTPKE
                     IKAFADFCKNFSFEVNGTHPLDKAFVTGGGVSTKEINPKSMESKLTKGLYFCGELIDY
                     NGYTGGYNITGAFVTGHTAGQHAAAGLHT"
BASE COUNT         1238 a         1451 c         1251 g         1384 t
ORIGIN      
        1 atttcgattt ctgcatcgac gcgttcggct gtaaaagtga ccaaaccttc ctgataagcc
       61 gcttcgccct gctggatttc cttccaagct tccccgaggg cctgttcacc gctcacgcgt
      121 tgactttcca actctgacca accattattg atctgtatct ggccatcctc cagttgcgag
      181 aaggccgatg ccaattccaa tcgaccattc tccaacgcaa tcccttgttc agcaaattga
      241 gcgtcagcag ctgcgatttg ggcttcggca tctgccatga atgccgcaat cttttcttcc
      301 agcagttgct gttgatccag caactcttcc cctctggcgg ctaattcgtt gccggccgac
      361 tcaagaaggc gctgttcctc catgagggcg gcctcttcac tggctcgttc tgcctgaaaa
      421 gcagcggctg cggcggtcaa ggcttcttgg cgggtggaca aggaggcacc ctcttgctcc
      481 agctcacttt ggcggacagt caattccgtt tcggcttgcg ccagcgcggc ccgttccgca
      541 tccaaagctg cctgctcttc atccagagca gttaagcgtt ctgccacgct cgggtccgtc
      601 tccggatcca gctcttccaa ggctgccctt tctgagtcga tagcggcttg tcgtgtggag
      661 aattccgctt gtttgattgc tttttccgtt tggaaagccg tcatgtcagc agtgaaaata
      721 gccagctctt tctggtaggc tgctgcatcc gcctccaaac tcatctgttc cgccaaaagc
      781 agctcgcctt ttgatgctaa ggccgctgcc cgttcctgca gctctagagc tttcatttcc
      841 agcgaacttt gggtcgcttc aacttcagcc tgcccgtcga gcagcatttg cgccagttca
      901 gcgatttcgc gtccttccgg tgtatcgggc aggcttgcca gcagcgcatc caatgaagcg
      961 tcggaatcgt tctgtccttg cagggctttt ttctgcgcct cccaggcaac ttttgcttca
     1021 tcgtagagcg ctttattttc cagataggtc gccaggcctg ctcggtactc cgccatgccc
     1081 gcatcgattt cagcttggct tgcagtcaaa gtgctctcgg cttgggcgat ttcgtccaga
     1141 aacaactggc gattctcctc atagagtgct gtgccgtcat ccaattgttg gcgggcatcc
     1201 tgcagttcct gctcggcttc cgcaagtttg tctttgccct cctgcagttc atcttccgca
     1261 tctctgatct tccgttgacc ctccgcacgg atttcattga tgcgctccaa cggtctcccg
     1321 ttcaaagcaa gctccacttc atcggccaat ctgtccactt gttcatcata ggcgtccgtg
     1381 tagcctgttt ccgccacagt cggagccatc gtcagataag cttccgtata gatgctgccg
     1441 agaatatccg attcgggtac gacaacgaaa gcatctaggc tgcccttccc gacattggtg
     1501 gaaccacgca ttatcgtttc gatgtacatc ggcgaatcaa cgaagccgac gaccgtgtat
     1561 tcttgttcct tcaaggagat gcggttttct ttttcttcat cactaaggtc ttcgtcggac
     1621 tcatggcgga aagtgatggt atcgccgatc tgataagtac tgctcagatt ctgtttcgca
     1681 tccaaggcga cttcgccgct tgtttccggc agtctgccgt ctactagcgc atatgcgttg
     1741 accggatccg caccgctttg cagcgggaat acttttacga gaaagtcact gccttccatt
     1801 tcgatgtcca ctgtccgcat cggatggaca gacagatcgg ccgtttcatc caaaatggcg
     1861 atgtccgctt cttccaatcc gtaggtagac aaaactttca ggtcatacag gttattgtct
     1921 tcataatatt ggttggcggt gtccagcata tccggacccg ccgctttgat gccggcaaag
     1981 aaagccacgc ccaaagtgat gatggccaat aaagcgagga aacgtgcctt acttttgcca
     2041 atctctatcc agatgtcttt ccatagcgcc ttcttcttca tcgtcttctc cccctcctac
     2101 cattcgattt ctgatactgg tttagggttt ggattttcat ggattgccct tactttagca
     2161 ttgttgattt cgatgatgcg gttcgccatg gctgcgatag cttggttgtg ggtgatgacg
     2221 atgacggttg ttcccgtatc aaggcatgta tcctgcagca gcttcagcac ttgtttgccc
     2281 gtttcgtaat ccagtgcccc agtcggctca tcacaaagca gcaacttggg ctgtttagcc
     2341 aacgcacggg cgatggcgac gcgttgctgt tcgcctccgg atagttgggc cggaaagtta
     2401 tccatgcgct cgcccaagcc tacttcccgc atgacctcgg cagcatcgcg ggcatgtggg
     2461 gatatttgcg aagcaagctc cacattctcc ttagcggtca ggttaggcac cagattatag
     2521 aattggaaga caaaaccgac atccctccgc ctgtaggtcg tcaacgcttt tgaagtgaac
     2581 tgtgagatat caaccccgtc gatgctgatg tgcccttcat cggcatcatc cattcctcca
     2641 aggatattca gaacagtgga ttttccggct ccactggggc ccagtatcac gacaaactcc
     2701 ccttcgttga tgtcgaagct gatcttgtcg ttggctgtga tccgagtttc gcccatctga
     2761 tagaatttgc tgacattctt taaggaaatg tacgccatta tgtatccctt catttcttga
     2821 gtgattttta ttttgaagca tcccggaaaa cttatccgta atacacttac ctatcgtaat
     2881 agtactatgg tatacaaaag cgcgcggata tgcaatcggc acctccacct ctggataaga
     2941 ccgccccgga cgagtatgca aaaaacgacc cgggaaactt gaacgtttct cctgcagtcg
     3001 tttttcattg tccaaactgt cctattcgac gatgttgacg gcgatctttt ccaatgtgcg
     3061 gggccgatca tgcagatagc cgtgcggata gcccgccacc attccggcga caggcgtaaa
     3121 accgtcggga atctgcattt tttcacggat cccttcagcg tccaacgtgc cgttcgccat
     3181 gattcccatc aggaagcagc tacctaaaag ttctgctgtt gcttgcagat gcatattttc
     3241 gacgatggtt gctgcgcttg cgatacttcc aggtttggcc ggttccttag cggaaacaac
     3301 gaaaaaggcc ggcgctccat acaaaggatc cttgtccgta ctgcccgtca cttcctttgc
     3361 cgccgatgta atcgctgcca atatttctgg attcttaatg actgtgatat ggtaatcctc
     3421 aaagcgaccg cggctgatcg gtgcattttg cccggcttgc agcatcagtt ccacttgccc
     3481 ttgggaaagt gcaaacttct ggaagctgcg tgttgtttgt ctcgtatgca ttactttcat
     3541 aaaatccatt tcaattccct cacttcttca aaaaacaact ctatactcat tatactcttt
     3601 cgggcataaa aatagaaagg aaatcctgca tagcagaaaa aaacgattat ctttgggtgt
     3661 agatacagta taggcgcaat ctctgaatat tttctgaata tttaatgaaa gaaagcatct
     3721 tttttcgtga cccttccgga aaaaaagcca ttttcagttg gacatatctg tgataaagta
     3781 agtgcagcaa aagaacggag aaaatacata acatgaaaac acgcatcatt attgtcggtg
     3841 gcggttcgag cggtctgatg gcggcttgca cggctgccga agcgggagcg gaagtgacgc
     3901 tcctcgaaca aaaccccagt ctcggaaaaa aattactctt gacaggcggg ggacgctgca
     3961 acgtcaccaa caaccgtccc gaagaagaaa tcatccggca cattcccgga aacggaaaat
     4021 tcctgcacag cagctttacc caattcagtc aatatgacat catgaccttt ttcatcgaac
     4081 gcggcgtcgc cctgaaggaa gaggatcacg gtcggatgtt ccctgtcaca gataaagcca
     4141 gaacaattct cgaagctttc atcgaggaac tgaagcggct gcaggtccag attcggacca
     4201 acataaaagt cgagcgtatc ctgatcgaaa atgaacaggt gaccggtgtc cttttggaag
     4261 atggcgagat tctcccggcc gatcggatta tcctggcaac tggaggaaaa gccctgccgc
     4321 gaactggatc caaaggcgac ggctataagt ttgccaaagc tgctggacat accatcacgg
     4381 aactataccc gaccgaagcg ccgataactt ccgatgcgga cttcatccgt gcaagcgaac
     4441 tgaagggact ttccttgcgc aacgttgcac tcagcatcag aaataaaaaa ggcaaaacag
     4501 tcgtgtccca ccagatggac atgatcttca cccacttcgg tgtgtccggt cccgcagctt
     4561 tgcgttgttc gatgtttgtc caccagacaa tgaagcgcga taaaaccgag acggtcacga
     4621 tgtccttgga tgtcctgccg gaactttcat tgggcgctgt cgaacaacaa ttgcaaaagc
     4681 tcatcaaggc ccaaccggaa aagagcgtca aaaacggctg gaaagacctc atgccggaac
     4741 gctacctgtt gttcggttgc acgcaggcgg ccatcgatcc gcagaagtca ttaaaggaat
     4801 tgactccgaa agaaatcaaa gctttcgcgg acttctgcaa aaatttctca ttcgaagtga
     4861 acggcacgca tccgttggat aaagctttcg tcactggcgg gggcgtctca acgaaggaaa
     4921 tcaatccgaa gtcgatggaa agcaaactga caaaaggact ttatttctgc ggtgaactga
     4981 tcgactacaa cggctacaca ggcggatata acatcaccgg cgcctttgtt acagggcaca
     5041 ctgccggaca acatgccgct gcgggtctgc atacataaaa atatcccata caaaaagaga
     5101 acggggcaac ctcccaatga ggcagctccg ttctcttttt tctatattgt aggaaaaatt
     5161 taaatttctc tgtactcaaa agtgcgtcac cgttggtgct tcaaagaact gcaaaatttt
     5221 ccatgcaatc caaagcgttg gctagcttca cgggttagcc cctcggaaag gatcaaggaa
     5281 ccccttgcct ctgcgagcca aggatactat tcgactaacc tgct
//
LOCUS       DAOMUJ010000071         5275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4320_length_5275_cov_2.622222, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000071 DAOMUJ010000000
VERSION     DAOMUJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4320_length_5275_cov_2.622222"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLT73_02930"
     CDS             <1..200
                     /locus_tag="PLT73_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRM19367.1"
                     /translation="ALAIIFSLTAILFPISSFWGTVVLGVGLIFGVVLFIASFNLLDS
                     NRSKLRKMLYRLLREKDEKNK"
     gene            complement(257..928)
                     /locus_tag="PLT73_02935"
     CDS             complement(257..928)
                     /locus_tag="PLT73_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRM19368.1"
                     /translation="MLTTYHTIAKSGVSEIVIKGSRFICSLKRVHSEDEAKAFIQAVK
                     KEHWKATHNCSAYLIGDQNEIQRAHDDGEPSGTAGVPMLEVLKKNDLRYVAAVVTRYF
                     GGTKLGAGGLIRAYSRSVSTALKDIGIVVRSLQTKVGCIVSYSASGKLENYLAQSAYT
                     LIEAQYTDQVCFLCGIPSEDISQFQSSLTDVMNGRVIFEIGEEDYVERPISPAYNDAD
                     EGEIE"
     gene            1090..2583
                     /locus_tag="PLT73_02940"
     CDS             1090..2583
                     /locus_tag="PLT73_02940"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM19369.1"
                     /translation="MKVTDLIDVLKMKKLIGSLDPELNIGKISQDSRDIQSGDLFICI
                     DGTQVDGHNYVETAVANGAGVIVAHKDIQKAAANVPVVYVPDTGKAMSLLANHFYGYP
                     SESMKVIGVTGTNGKTTVTYLVEAILKAMDKKTGLMGTIEMHIGDEVHKTKNTTPDSI
                     TMQKALHLMTERETEYFTLELSSIALEMGRAWGLDLDVAVMTNLTHEHMEFHHTMAAY
                     AEAKKLLFSQLGNGRKNGRTKAAILNADDETIHSYRIATAAEVISYSVKDETADFFAT
                     DITYSRTQTRFNLVVNGERYPVVTNLVGLYNVSNTLAALAAVYALGIPLEDATKAVTS
                     LAGVIGRLEIVPGANDIGVYVDFAHSPDAMEKVLGVVREFTQGRVISVFGGAGEREHE
                     KRPIMARIGTELSDYVILTTDDTGKEPQEQIIAMMLAGIEKDNYEYIENRKEAIQHAI
                     AIAEPGDTVILLGRGHEADYNDRGTMIRLLDSEVAAEAIELRNEQKV"
     gene            2591..3457
                     /locus_tag="PLT73_02945"
     CDS             2591..3457
                     /locus_tag="PLT73_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM19370.1"
                     /translation="MKIAVVTDSTAYLTAETRQQHHIHMLPLVVNIGAKSYQEEIDLA
                     AEDFYSLMKSAEDFPKSSQPAVGAMHQLYEELAKEHDAIISIHLSSGISGTYQNAAAL
                     SREYPEFNIHPFDSEISCYVQARFVLEAARLAAVGIGPEQIMARLEHMKKRSRAYFMV
                     DDLMNLQRGGRLSGGAAVIGSIMKIKPVLHFSDKKIVVFEKIRTNARALRRIEELLGQ
                     AASKADYPIIATVIHGNIPEKGQAWLEHLQQEFPDIRFELSYFGPVIGTHLGEGALGL
                     TWTEDTELSYPI"
     gene            3558..4157
                     /gene="thpR"
                     /locus_tag="PLT73_02950"
     CDS             3558..4157
                     /gene="thpR"
                     /locus_tag="PLT73_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRM19371.1"
                     /translation="MKVFIGVKLPSAVKKQLVDFQKEVIRASIKGSFTDPNNFHLTMR
                     FIGEVDPDQRSAIETGLARCAESQLPFLVETNGLGYFSKRNKWIVWLGTKENSRLQQL
                     YEQLNDSLLGQKVAMADEVAFIPHLTLGRGIVLWQEWEELSKVPLPPDQKITVAALTL
                     FESVRLDGKLVYRPIADFPFAGQAAATDRIDSREETEKS"
     gene            4238..>5015
                     /locus_tag="PLT73_02955"
     CDS             4238..>5015
                     /locus_tag="PLT73_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRM19372.1"
                     /translation="MDGKELYGRELTRSECRNAEEALLILAEKRPGLTSANGKRICNR
                     CGNQDRKKMLAAPCACGTTCFYCLSCLNMGKIKSCTVLHHLPEINAFERPIEPILQWQ
                     GQLSSEQQRASEEIVETVQAEETRLIWAVAGAGKTEMIFEGIAACLRKGGRVCLASPR
                     VDVCLELAPRIKQAFPAVPMALLYGGNEDGYSYTPLVIATTHQLLRFREAFDLLIIDE
                     IDSFPYHDDLGLQFGAQKSRKKASALIYLTATPSQMMQNDI"
     assembly_gap    5016..5025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5026..>5275
                     /locus_tag="PLT73_02960"
     CDS             <5026..>5275
                     /locus_tag="PLT73_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRM19373.1"
                     /translation="RDKLAATVLPARYHGFALPEPECLWIGDWRKAISKKKMAPFLKL
                     IRRQLQTDRRFLLFLPHIQLMEELDGWLRELFPDKQFTC"
BASE COUNT         1367 a         1234 c         1437 g         1227 t
ORIGIN      
        1 atgctttggc gatcatcttt tctttgaccg ccatcctttt tccgatttct tctttctggg
       61 gaacggtggt tttgggagtc ggtctgattt ttggtgtagt gctcttcatc gcctccttca
      121 atttgttgga cagcaaccgt tccaaattgc gaaaaatgtt gtatcgcctg ctgagggaaa
      181 aggacgaaaa aaacaaatga acaagctatg agagcctagg tggctgtcat gacttgttca
      241 ttttttcgtt cagattctat tcgatttcgc cttcatcagc atcattgtag gctggagaaa
      301 tcggacgctc cacataatcc tcttcgccga tttcaaagat aacacggccg ttcatcacgt
      361 ccgtaaggga gctctgaaat tgactgatgt cttcagaagg gattccgcac aagaaacaaa
      421 cttggtcggt gtattgtgcc tcaatcagcg tgtacgccga ttgcgccaag taattctcta
      481 attttccgga tgcggaatag gaaacgatgc agccgacttt agtctggagg ctgcggacaa
      541 cgataccgat gtccttcaag gccgtcgata ccgatcggct gtaggcacgg atcaatccgc
      601 ccgcacccaa ttttgtcccg ccgaaataac gggtgacgac cgcagcgacg taacgcaaat
      661 catttttctt gagtacttcc agcatcggga caccagctgt tccggatggc tcgccgtcgt
      721 catgggcgcg ttggatttcg ttctggtcgc cgatcaggta tgcggaacag ttatgcgttg
      781 ctttccaatg ctcttttttg acggcttgga tgaatgcttt ggcctcgtcc tcggaatgga
      841 cgcgtttaag gctgcagatg aagcgtgaac ccttgatgac gatctcggaa acgccgctct
      901 ttgcgatagt gtgatacgtt gtcagcatag ctattatcct ccaataagtt attcagtccc
      961 attttaccac agcagcggta ggatgacagc gcacgaacaa ggaaagggac tttccgaaca
     1021 tttccatttg tggtattgtt aaaaggaata gaccaaacgg ccccagaggt cgatggaaag
     1081 gtgatctgcg tgaaagttac agatttaatc gatgtactca aaatgaagaa acttatcggg
     1141 agcttggatc ccgaattgaa tattggaaaa atcagtcagg actcccgtga tatccaatca
     1201 ggggatctgt ttatctgcat cgacgggacg caagtggatg gccacaatta cgtagagaca
     1261 gcggtcgcca acggtgcggg tgtgattgtt gcccacaagg atattcaaaa agcggcagcg
     1321 aatgtcccgg ttgtctatgt tccggatacg ggtaaagcga tgagtctgct tgcgaatcac
     1381 ttttatggtt atccgagtga atcgatgaaa gtcatcggcg tcacagggac aaacggaaag
     1441 acgacagtca cgtaccttgt ggaagccatc cttaaggcga tggacaaaaa aaccgggttg
     1501 atgggcacca tcgaaatgca tatcggtgac gaggtgcaca aaacgaaaaa tacgacgccc
     1561 gacagcatca cgatgcaaaa agccctgcac ctcatgacag agcgggaaac ggaatacttc
     1621 actctggaac tgtcctcgat cgctttggaa atgggcagag catgggggct ggacctggat
     1681 gtcgccgtca tgaccaacct gacgcatgaa catatggaat tccatcacac gatggcagct
     1741 tatgccgaag cgaaaaagtt gctcttctcg caactgggca acggccgcaa aaacggacgg
     1801 acgaaagcgg cgatactgaa tgcagatgat gaaacgatcc acagctatcg catcgctacg
     1861 gcggcggaag tcatttctta tagcgtgaag gatgagacag ctgatttctt cgcgacggat
     1921 atcacctaca gccgcaccca gacccgcttt aatctggtcg tcaatgggga gcgctatccg
     1981 gtcgtaacga atctggtggg actttataat gtctccaaca ccttggctgc gttggcggcg
     2041 gtctatgctt taggcattcc tttggaggat gctacaaaag cggtgacgtc attggctggc
     2101 gtcataggca ggctggaaat cgttcctggc gcgaatgaca tcggtgtcta tgtcgatttt
     2161 gcccattcgc ccgacgcgat ggaaaaagta ttgggcgtgg tgcgcgaatt cacgcaagga
     2221 agggtcattt ccgttttcgg cggtgcaggg gaacgggagc atgaaaaacg tccgattatg
     2281 gctcgcatcg gtacggaact ctcggactat gtgatcctga cgacggacga taccgggaaa
     2341 gaaccgcagg aacaaatcat cgcgatgatg ctggccggca tcgaaaagga caattatgaa
     2401 tacatcgaaa accgcaagga agccatccaa catgccatcg ccatcgcaga accgggagat
     2461 acggtcatcc tgttgggacg tggacatgag gctgactaca acgatagagg cacaatgatc
     2521 cgcctgttgg acagtgaagt ggcggctgaa gcgattgaat tgcgcaatga gcagaaagtt
     2581 tgaggattct atgaaaattg cagtagttac ggacagtacc gcatatttga cggctgaaac
     2641 acgccaacag caccatattc acatgctgcc gttagtcgtg aatattggag caaaatcgta
     2701 tcaggaagaa atagatttgg ctgcagagga cttctactcc ttgatgaaat ctgctgaaga
     2761 tttcccgaaa agttctcaac ccgcagtggg tgccatgcat cagctgtatg aagaattggc
     2821 aaaggaacat gatgcaatca tcagcatcca tctttcaagc ggcatcagtg gcacatacca
     2881 gaatgctgct gctttaagca gggaataccc tgaattcaac attcaccctt ttgattctga
     2941 aatcagttgc tatgtgcagg caagattcgt tttggaggca gcaaggctgg cggctgtcgg
     3001 tatcggaccg gagcagatca tggcgcgttt agaacatatg aagaaacgtt cccgtgctta
     3061 ttttatggtg gacgatctga tgaacctgca acgtggtggc aggctttcgg gtggagcggc
     3121 cgtcatcggt tccatcatga aaatcaagcc agtgctgcat ttttctgaca aaaagatagt
     3181 tgttttcgaa aaaatcagaa ccaatgcacg ggcgttgcga aggatagaag aacttttggg
     3241 tcaagcggct tcaaaggctg attatccgat tatcgcaact gtcatccatg ggaatattcc
     3301 cgaaaagggg caggcttggt tggaacacct ccagcaagag tttccggata tccggtttga
     3361 attgagttat ttcggtccag tcatcgggac gcacttaggt gaaggcgcgc tggggttgac
     3421 ctggacggaa gataccgaat tgagctatcc gatataagca acaaataggt gcggcaatca
     3481 cacattcatt cgggaggaag ggccaataag gttctgtcct tcctgttttt ttacaaagcg
     3541 gatagcgggg tgcagtcgtg aaggtattca tcggtgtcaa attgcctagt gccgtcaaaa
     3601 aacagttggt tgatttccag aaggaagtga tccgcgccag catcaaaggg tcattcacgg
     3661 atccaaacaa cttccacttg acgatgcggt tcatcgggga agtggacccg gatcagagaa
     3721 gtgcaatcga aacggggctg gcccgctgcg ctgaaagtca gctgccattc ttggttgaaa
     3781 cgaatggtct gggttacttt tccaaaagaa acaagtggat cgtctggctg ggcaccaaag
     3841 aaaacagccg actccagcag ctctacgaac agctgaacga ttccttgctg gggcaaaaag
     3901 tagcaatggc tgatgaggtg gcgttcattc cgcacctcac gctcggccgg gggattgtat
     3961 tgtggcaaga gtgggaagag ctcagcaaag ttccgctgcc cccggatcaa aaaataaccg
     4021 tcgctgcgct gacgctattc gaaagtgttc ggctggacgg aaaacttgtt tatcgcccaa
     4081 tcgctgattt tcctttcgcc ggacaagctg cagcaaccga ccggatcgat tcccgagagg
     4141 aaactgaaaa atcctaagcc gtcccatttc gccgatcagc ggaagcggag cggctttttc
     4201 tgcgtatgtt aataaggaaa gcgaaaggga ggatgatatg gacgggaagg aactgtacgg
     4261 cagggaattg acgaggagcg agtgccgcaa cgccgaagaa gcgctgttga tcctggctga
     4321 gaaacgcccc ggattgacca gcgcgaacgg gaaacggata tgcaatcgct gcggaaacca
     4381 ggaccggaaa aaaatgctgg ccgcgccttg cgcttgcggc actacttgtt tttactgctt
     4441 gagctgcctg aacatgggaa aaatcaaaag ctgcacagtg ctccatcatc tgccggaaat
     4501 caatgctttt gaacggccca ttgagccgat tctgcaatgg cagggccaat tgtcatcgga
     4561 acagcaacgt gcatcggaag aaatcgtaga aacggtccaa gctgaagaga cacgactgat
     4621 ctgggccgtc gctggtgccg gaaagactga gatgattttt gaggggattg cagcctgcct
     4681 gcgcaaaggt ggtagagtct gtcttgcctc cccgcgcgtg gatgtttgtt tggaactggc
     4741 accccggatc aagcaggcat tcccggccgt tccgatggcc ttgctgtacg gaggcaatga
     4801 ggatggttac agctatacgc cgctcgtcat cgccacgacc catcaactgc tgcgcttccg
     4861 cgaagcgttc gacctactga tcatcgatga gatcgattcc ttcccgtatc acgatgattt
     4921 gggcctgcag ttcggggcgc aaaagtccag aaaaaaagcg agtgcgctga tttatttgac
     4981 tgctacgcct tcacagatga tgcaaaacga catcannnnn nnnnncgaga caagttggcg
     5041 gcgacagtct tgccggcgcg ttatcacggc tttgcattgc ccgaaccgga gtgtctgtgg
     5101 atcggagatt ggcgcaaggc catcagtaag aagaaaatgg cccctttcct taagcttatc
     5161 cgcaggcaac tccaaacgga caggcgcttt ctgctgtttt tgccgcacat ccagctgatg
     5221 gaagaactgg acggttggct gcgggaactt tttccggaca agcaattcac ctgcg
//
LOCUS       DAOMUJ010000072         5245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4354_length_5245_cov_2.316378, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000072 DAOMUJ010000000
VERSION     DAOMUJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4354_length_5245_cov_2.316378"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>322)
                     /locus_tag="PLT73_02965"
     CDS             complement(<1..>322)
                     /locus_tag="PLT73_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRM19374.1"
                     /translation="NAKVAQTMAKMVDFGGNTEDAVVALCAALFAIVVWATAAWYFGI
                     PTSESHALIAGVSGAAIALQGGIDGINGAEWIKVLYGLLLSTVLGLSLGFLVVKLVGA
                     LFKDI"
     assembly_gap    323..353
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<354..558)
                     /locus_tag="PLT73_02970"
     CDS             complement(<354..558)
                     /locus_tag="PLT73_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006729164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRM19375.1"
                     /translation="MAISFGSFIQLLLTSPAIAVTVILTLGVVLVNGWTDAPNAIATC
                     VSTRSMTPRNAILMAAVFNFFGVF"
     gene            complement(601..>1926)
                     /locus_tag="PLT73_02975"
     CDS             complement(601..>1926)
                     /locus_tag="PLT73_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HRM19376.1"
                     /translation="AAFVCAGNALEQNGATLTADDLRPYLTHPTVIGLAEVMDYPAVL
                     DADPDMLEKIRMTTEAGMMVDGHAAGLTDTALNVYSTTGIRNDHEAVTAEEAMARVRR
                     GIHVLVREGSAAKDLLELLPAINERNSRRFSFCTDDKHLDELAEEGTINYAAQLAIQQ
                     GLDPLIAIQMATLNNAVCHGIQDKGAIAPGYVADILITDSLERLSPETIIKDGRVLNL
                     DELRNVHAVVPQAVRSSLHLKKVTKVDLQIPLLEGQKAWVIGIVPGSIITQKNARDVQ
                     TEDGFFVADPQNDQLKMVVCERHHQTGSIGAGIVSGLGLKRGAIASTVAHDSHNLVVA
                     GTNDEDMLLAIEEAERMQGGLVIVDGGKVLASVPLRVGGIMSEKPYEEVIKELHQLHG
                     QLATLAEEGQNIFMILSFLCLPVIPRLKLTDKGLFDVDSFQHIEVGIPM"
     assembly_gap    1927..1936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1999..2496)
                     /locus_tag="PLT73_02980"
     CDS             complement(1999..2496)
                     /locus_tag="PLT73_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM19377.1"
                     /translation="MPRLRCLSAPTRIVYNIRKNKRFADQESEMEPGGICMGDGLKRL
                     IQAAQQLEEADLIIQNGTVVDVFSLETFEADVAIRDGRIVGIGTYPNGKQVVDATGKF
                     ILPGFIDGHIHIESTMVTPSQFSRALIKHGITTVVTDPHEIANVAGTIGIDFMLEDAA
                     NADTA"
     gene            2727..3572
                     /locus_tag="PLT73_02985"
     CDS             2727..3572
                     /locus_tag="PLT73_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM19378.1"
                     /translation="MKKLLSKILFVGTAGLALAACGATTTDSSSDSASTASSTAATAN
                     VLKTIEENKQLRIGVEGTYPPFSYHDTETGDLVGFEVEIAEVIAEDLGVEPVFVETKW
                     DSLIAGLDVNKYDIVINNVGVTEERQAVYDFTDAYFESYGQLAVSADSDIQTLDDYSG
                     KKSAQSTTSNYAQNARDLGAEIVPVDGFNQAVELITSGRADGTINDYITFLTYFEEYP
                     DSTLRLIDEKLPTNDKVGIILQKENTDFQTKLNEIIAARTEDGTFKAIFEKYLGEDIS
                     VGANN"
     gene            3805..4473
                     /locus_tag="PLT73_02990"
     CDS             3805..4473
                     /locus_tag="PLT73_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM19379.1"
                     /translation="MQHTLSIIENALWPILEAGFTVTIPLALISFALSLVLAVLTAVM
                     RISKSRLLKGMAWFYVWVFRGTPLLIQLFIVFFGLPKLNITLDAWTAAILTFALNSGA
                     YSAESIRGAILSIPRGQWEAADSLGMTHGQVLKRIIFPQTVRIALPAVMNDFISLVKS
                     TSLASSITIIEMTMVGQQFASVTYEPLILYMEVAVIYLLISTVLTWIQGKLEIRTSKY
                     LKKA"
     gene            4478..5215
                     /locus_tag="PLT73_02995"
     CDS             4478..5215
                     /locus_tag="PLT73_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM19380.1"
                     /translation="MIEIKHIQKSYGDFHALKDISLTFKTGETTVIVGPSGSGKSTLL
                     RTLNLLEVPESGILKNGTLEVDFSSKISKETMMAIRKETAMVFQSFNLFPHLSVLENV
                     IEGPITVLKLSKSEATARAKNLLTKVGLADKLDHYPDQLSGGQQQRVAIARALAMEPD
                     FVLFDEPTSALDPELEAEVLRVLKELASEKLSMIIVTHNLDFAREAADRMLFLEDGKV
                     GFDGPTKDFFASTTDPRIARFINSITI"
BASE COUNT         1342 a         1418 c         1188 g         1256 t
ORIGIN      
        1 gatgtctttg aacagcgcgc cgacaagctt cacgaccaga aagcccagag acaacccaag
       61 aacggtggaa agcagcaatc catacagaac cttaatccat tccgcaccat tgattccatc
      121 aataccgcct tgcaacgcaa tcgcagcccc agacacccca gcaatcaagg catggctttc
      181 acttgtcgga atgccaaaat accaagcggc agtggcccat accacaatcg caaagagcgc
      241 cgcacacagc gccacgaccg catcttccgt atttccgcca aaatcaacca tcttcgccat
      301 cgtctgggca actttcgcat tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaagac
      361 accaaaaaaa ttgaataccg ccgccatcaa gatggcattg cgcggggtca tacttcgtgt
      421 cgaaacgcag gtggcgatgg cgttcggcgc atccgtccac ccgttcacca aaaccacgcc
      481 caacgtcaag atcaccgtca ccgcaatagc cgggctcgtc agcagcaact gaatgaatga
      541 accaaatgaa atcgccataa aggaccaccg cctttccgga ccgcactgaa gtaagtgacc
      601 ttacatcgga atccccactt cgatatgctg gaaggaatcc acatcaaaca atcctttatc
      661 cgtcaatttc agccgcggaa tgaccgggag acacagaaac gaaagaatca tgaaaatgtt
      721 ctgtccttcc tccgccagcg tagccaactg cccgtgcaac tgatgcaatt ccttgatcac
      781 ttcttcatac ggtttttccg acataatacc tccgacccgc agcgggactg aagccaatac
      841 tttgccgcca tctacgatga cgagtccgcc ttgcatccgt tccgcttcct cgatcgccag
      901 cagcatgtct tcatcgttgg ttccggccac gaccagatta tgcgaatcat gggctactgt
      961 cgaagcgatt gcgccccgtt tcagacctaa accactgacg atgccagcac cgatgctgcc
     1021 ggtttggtga tggcgctcgc acacaaccat cttcagttga tcattttgcg gatccgcaac
     1081 aaaaaatccg tcttccgtct gcacatccct tgcgtttttc tgtgtaatga tgcttcccgg
     1141 cacaatgccg ataacccaag ctttttggcc ttccaagagt ggaatctgca agtcgacttt
     1201 tgttactttc tttaaatgaa gcgatgatcg tacagcttgc gggacaacag catggacatt
     1261 tctcagctca tccaaattca gcacgcgccc gtctttgatg atcgtttccg ggctcagcct
     1321 ctctaaactg tccgtaatca agatgtccgc aacataacct ggtgcgatgg cgcctttgtc
     1381 ctggataccg tgacacaccg cattgttgag cgtagccatc tggatcgcga tcagcggatc
     1441 taagccttgt tggatcgcaa gctgggcggc ataattgatg gttccctctt cggctaactc
     1501 gtctagatgc ttatcatccg tacagaatga aaaacgacgg ctgttccttt cgttgatggc
     1561 cggcaacaac tccagcaaat ctttcgcggc tgaaccttcg cgcaccaata cgtgtatgcc
     1621 acggcgtacg cgtgccattg cttcttccgc cgtcaccgcc tcgtggtcat tgcggattcc
     1681 ggttgtgctg tacacattca gcgcagtatc agtcaaaccg gccgcatgtc catcgaccat
     1741 catcccagct tctgtcgtca tgcggatttt ttccagcatg tccgggtctg catccaagac
     1801 agctggataa tccatcactt ccgccaggcc aatgacggtc ggatgggtca aataagggcg
     1861 gagatcatcc gcagtcagcg tcgcgccgtt ctgctcgagg gcgttgccgg cacacacgaa
     1921 ggcagcnnnn nnnnnncaat ggtcgatacg tgggcgacgg tggggtcatg tgcccaaatc
     1981 ggtaaaaacg tgcatttatc aggcggtgtc ggcattggcg gcgtcctcga gcataaagtc
     2041 gattccgatt gtccctgcga cgttcgcaat ttcatgagga tcggtcacaa cggtcgtaat
     2101 accgtgtttg atgagcgccc gtgaaaattg cgacggtgtc accatcgtcg attcaatatg
     2161 aatatgccca tcgataaaac ccggcagaat gaacttgcct gtagcatcca ccacttgctt
     2221 tccgttcggg taggtaccga tgccgacgat acgaccgtcc cggatcgcga cgtcagcctc
     2281 aaaggtctca agcgaaaaca catccactac ggttccgttt tgaatgataa gatccgcttc
     2341 ctccagttgt tgcgccgctt ggatcaacct tttcaatcca tctcccatac aaattccccc
     2401 tggttccatt tcgctctctt gatcagcgaa tctcttgttt tttcttatat tatacacgat
     2461 tctcgtgggg gccgataagc atcttaatcg cggcatgatt ttcatgatgc ataatactta
     2521 ggtgatcggt tttacctatc aatactgttc gtttttaact atttgcttct tacatacgct
     2581 ggtgataaga tttcctcatg ccttcgagcg acaaaaagta aggaatttcg gtttacggtc
     2641 caccatcacc agcaataacc atgtggggcg ttttttcggt cgaatgaccg gcaactattc
     2701 aaaaaaaaca acaggagtga atattaatga agaaactatt atcaaaaatc ttattcgtgg
     2761 gtacagccgg cttggctttg gcggcttgcg gcgcaacaac aaccgactca agttcggaca
     2821 gtgcctctac agcatccagc actgccgcaa cagcaaatgt cttgaagaca atcgaagaaa
     2881 acaaacaatt gcgcatcggg gtggaaggta cttaccctcc gttcagctat catgatacag
     2941 aaaccggaga cttagtcggt ttcgaagtgg aaatcgcaga agtcattgcg gaagatttag
     3001 gtgtggaacc tgtctttgtc gaaacgaaat gggattccct gattgccgga ttggacgtca
     3061 ataaatacga tatcgtcatc aacaacgtcg gcgtgaccga agaacgacaa gcggtctacg
     3121 acttcaccga tgcttatttc gagtcctatg gccaattggc ggtcagtgca gactccgaca
     3181 ttcaaacttt ggatgactac agcggcaaaa aatcagccca atccactaca agcaattacg
     3241 cacagaacgc ccgcgacctt ggtgctgaaa tcgtccctgt cgacggcttc aatcaagcag
     3301 tcgaattgat cacttcaggc cgtgcagacg gaacgatcaa tgattacatc actttcttga
     3361 cttactttga agaatatcca gactccactt tgcgtctgat tgatgaaaaa ctgcctacaa
     3421 acgataaagt cggcatcatc ctgcaaaagg aaaacacaga tttccagact aaactgaatg
     3481 aaatcatcgc agcccgcaca gaagacggca cttttaaagc aatcttcgaa aaatacctag
     3541 gcgaagacat cagcgtcggc gcgaacaact aagaaaagct gaacgggctc gttccgtctc
     3601 acatatacga aatcgtaccg attgcgcaga ttagagctca atcggtacga tttatctgat
     3661 ttatggatgg ttaatccttg aagctagcca tcatcgttgg atgcgtgtta caatttggta
     3721 tctcgtgaac cacttgcggt gatgcgcttt tggacattga atagtttttt aatttttaat
     3781 ataaaaagga acggtgagta aattttgcag catacattat ccataatcga aaacgcccta
     3841 tggccaattc tggaagcagg gtttaccgtc accatcccct tggcgctgat ttcctttgct
     3901 ttgtcccttg tactggcagt ccttactgca gtcatgcgga tttcgaaaag caggttgttg
     3961 aaagggatgg cttggttcta cgtttgggta ttccgcggca caccgctttt gatccaattg
     4021 ttcatcgtct tcttcggttt gccgaagctg aacatcacct tggatgcgtg gaccgccgct
     4081 atcctgactt ttgccttaaa cagcggcgcc tattcggccg aatccatccg cggcgcgatc
     4141 ctgtccatcc cgagagggca atgggaagcg gctgactcct tgggaatgac gcacggacag
     4201 gtcctgaaaa ggatcatctt cccgcagacc gtccggattg ccttgccggc tgtcatgaac
     4261 gacttcattt ctctggtgaa atcgacttcg ctggcatcca gcatcacgat cattgaaatg
     4321 acgatggtcg ggcagcaatt cgcttccgtt acctatgaac cactgattct gtatatggaa
     4381 gtggctgtga tctacctcct tatcagtacc gtgttgactt ggatccaggg caaattggaa
     4441 atcagaactt ccaaatatct gaaaaaggcg tgacgacatg atcgaaatca aacatatcca
     4501 aaaatcctac ggtgactttc atgccctcaa ggatatctca ttaactttca aaactggcga
     4561 aacgaccgtc atcgtcgggc catccggttc aggcaagtcc accttgctcc gcaccttgaa
     4621 cctgttggaa gtgccagaaa gcggcatcct gaagaacggc actctcgagg tcgatttcag
     4681 ctccaaaatt tcgaaagaaa caatgatggc aatccgcaaa gaaacggcga tggtgttcca
     4741 gagtttcaac ctcttcccgc atctgtcggt attggaaaat gtcatcgaag gcccgattac
     4801 ggttctgaaa ctttcgaaat cagaagcgac ggccagagcc aaaaacctgc tgaccaaagt
     4861 cggcttggcc gacaaactgg accattaccc ggaccaactg tcgggcggtc aacaacaacg
     4921 cgtcgccatc gcgcgcgcct tggctatgga accggatttc gtcctgttcg atgaacccac
     4981 ttccgccctt gatcccgagt tggaagcgga agtgctgcgc gtcttaaagg agctcgcttc
     5041 cgaaaagcta tccatgatca tcgtgacgca caaccttgac ttcgcacggg aagcagcgga
     5101 ccgcatgctc ttcctggagg acggcaaagt cggcttcgac ggcccgacca aggacttctt
     5161 cgcaagcaca accgacccaa ggattgcacg tttcatcaac agcatcacga tttaaaaaga
     5221 tagacgatac cagagcttgg atgct
//
LOCUS       DAOMUJ010000073         5217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4385_length_5217_cov_1.818287, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000073 DAOMUJ010000000
VERSION     DAOMUJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_5217_cov_1.818287"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    236..245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<246..1094)
                     /gene="aroF"
                     /locus_tag="PLT73_03000"
     CDS             complement(<246..1094)
                     /gene="aroF"
                     /locus_tag="PLT73_03000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM19381.1"
                     /translation="MTKRLTDKGVIVSPVIGETMTILGLVGDTSKVSMDTIQQHHIVE
                     RVLKVQEPYKKANRKFHPDDTVVEIDGQRIGNGYFGVIAGPCSVEGEEQIISIAQRAK
                     AAGANFLRGGAFKPRTSPYSFQGLELEGLRLLKLAKEATGLPIVTELMSTKFVDAFEA
                     DVDMIQIGARNMQNFDLLKEVGKTKTPVLLKRGLSATYDEWLMSAEYIMSEGNENVIL
                     CERGIRTFETETRNTLDIQAVPVIQKLSHLPIVIDPSHAGGSAYLVPSMSKSAVMSGA
                     NGLMVEI"
     assembly_gap    1099..1139
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1601..>2249
                     /locus_tag="PLT73_03005"
     CDS             1601..>2249
                     /locus_tag="PLT73_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRM19382.1"
                     /translation="MQFFGLFGEHLSHSYSERIHSTFFDLIGMNAGYKHIELSPIELE
                     AAIEGIRVLQFVGVNVTIPYKQTVMPYLDEITPEATRIGAVNTIKVKEGKLYGANTDY
                     YGFGTMLASHSIAVENQTAMVLGFGGSAKAVVLYLLDNGINNIIIVSRKKGALTDIPD
                     DPRISLMSYDEITDISGDLIINTTPLGMFPDKIGISAVPLEVICHFSAAVDLIYNP"
     assembly_gap    2250..2259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2420..2950
                     /locus_tag="PLT73_03010"
     CDS             2420..2950
                     /locus_tag="PLT73_03010"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM19383.1"
                     /translation="MGKNIILIGMPGAGKTTLGKLLSNITGFRFIDSDAFIEEKTGES
                     ITDLFQIGEDYFRNIESDAYKEIADSEDTIVSTGGGVVKRSENIVALKKSGTVYYIDR
                     AVEDITHDIEVSHRPLLKDGLERLHALKAEREALYQDAADFVVKNDKPLEEIAAEIVA
                     CHFGVGKEGSAHDNPQ"
     gene            2934..3794
                     /locus_tag="PLT73_03015"
     CDS             2934..3794
                     /locus_tag="PLT73_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRM19384.1"
                     /translation="MTTLNKELNITIVGLGVIGGSFAMALHDAGYRNLYGVDIDQATL
                     DKAKAQRLIKEGYQDGKDILPQSDLVIFSLYPRLVHDFIVTHKQYFKPGSILTDATGV
                     KKFFIEDILSVLPDSIDFVFGHPMAGREKKGIDFASSSVYKNANYILTPLPSNKEENL
                     AFMEALIMEMGFGRITRISADYHDEMIAFTSQLPHAIAVALVNSDVEGRETEKFTGDS
                     YRELTRIAKINEELWSELFLENKEYLLKSIQTFEDQLDLIKAAIKNDDSVALKQLFIQ
                     STKRREKFDR"
     gene            3957..4679
                     /locus_tag="PLT73_03020"
     CDS             3957..4679
                     /locus_tag="PLT73_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19385.1"
                     /translation="MREKLKKFSYLVILFIFASFILITGYEFVRFLQTRGTEKQTEHF
                     LRLVQAGFGLVALLFPSLLRKHTRILLPQRITFIYAVFLYLALLLGSLGGFYDTVAEW
                     DTLQHALSSALFAVLGFSVIANLQEGGIERLNLTPVLSSLFSFCLATTLGVLWEFYEY
                     FADSVMGQNTQRTATVDGIPLIGQAALSDTMGDLIVNCSAAAIVAILGYFALKSSGNW
                     MESYLITFDTEPENNKRIRGKK"
     gene            4676..5119
                     /locus_tag="PLT73_03025"
     CDS             4676..5119
                     /locus_tag="PLT73_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19386.1"
                     /translation="MITIRNLSIDNIEDALALSVDDWQRKYVRPNAEMIALAYVGPQE
                     LFPIALYAGEELVGLAFVKKEQKAAIMTLEQIMIGHPFQSKSFGSESLREIAKWIETQ
                     FSCSLLQASVQIGNQWGRETLENAGFMKRATDLEKREIEMIYIIK"
BASE COUNT         1389 a         1229 c         1219 g         1319 t
ORIGIN      
        1 aattcagcca ttgaatcact ccttattcag attaggttaa aagaaaaacg gaacgggttc
       61 tttccaggct tacccgtgaa gctagccaac gcattgaaat gcgccgaaag tgtttccttt
      121 attttaccgc atccaaatgc tttccttcaa tggcaatcaa ctgttttacg acagccatca
      181 actcatcgaa ctcattcggg ttcagacact gttggccatc actccatgcg ttctcnnnnn
      241 nnnnnaattt cgaccatcag accgttcgcg ccggacatga ctgccgattt ggacatggat
      301 ggcacgaggt aagcagaacc gcccgcgtgg cttggatcga tgacgatcgg caagtgggat
      361 aatttctgga tgaccggaac ggcttggata tccaaggtat tgcgtgtttc cgtttcaaat
      421 gtgcggattc cacgttcgca aaggatgacg ttctcgttcc cttcggacat gatgtattca
      481 gcagacatca accattcatc gtaagttgca gacaaaccac gcttcaacag gacaggcgtc
      541 ttcgtcttgc cgacttcttt caacagatcg aaattctgca tgttacgggc gccgatttgg
      601 atcatatcga catccgcctc aaatgcatct acgaatttcg tggacatcaa ttcggttacg
      661 atcggcaagc ccgtcgcttc cttcgccaat ttcaataaac gcaagccttc taattccaaa
      721 ccttggaagc tgtatgggga tgtgcgcggc ttgaaggctc caccgcggag gaagttagcg
      781 cctgccgctt ttgcgcgttg cgcgatgctg atgatctgtt cttcaccttc aacggaacaa
      841 ggccctgcga tcactccgaa gtatccatta ccgatacgct gtccgtcaat ttcgacgact
      901 gtatcgtccg gatggaattt acggtttgct tttttgtaag gctcttgaac tttcaacacg
      961 cgttcgacga tatgatgttg ctggatggtg tccatcgata ctttcgaagt gtcccctacc
     1021 aaacccaata tcgtcattgt ttcgccgatt accggactca cgatgacccc tttgtctgtt
     1081 aagcgttttg tcagttcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1141 tgatcatgtc ttgttcctcc tagtatgttt ttgctttaaa aaaataaaaa gcggtgccca
     1201 tatgatatga gcaccgcgtt aagcttcgtt atccaaaaat gctgttgcct gacgatccgt
     1261 cctgcaaact attttccgca tcccctgatg tttctcatat tcgctctatg caattttttc
     1321 caaacaaaat atccagcgct aaaatagaag ccccagaatg taaagtatgt aaagtaaaag
     1381 tttgtttgta aaagattgat agcatgcgtg gaacccatcg gatgacctct tctcttgtgt
     1441 atcatttaac cagctgaact cataatgaaa acgactgatt tccatcatta taacaaacgc
     1501 tctaatcatt gcaagagttt tttaattttt ttttaattta atctgggtat tgaaatttag
     1561 atggcgataa tctacacttt cctaaaagga gtgaaacctg atgcaatttt tcggactatt
     1621 cggcgaacat ttgagccaca gctattcaga aaggatccat tcgacttttt ttgatctgat
     1681 cggaatgaat gccggttata aacatattga attatcccct atcgaactcg aagcagcgat
     1741 agaaggaatc cgcgtgcttc agttcgtcgg agtcaacgtg acgattccct acaaacagac
     1801 ggtgatgcct tacttggatg agatcacccc cgaagcgacg cgcatcggtg ctgtcaacac
     1861 gatcaaggta aaagaaggta aactgtacgg tgctaatacc gattattatg gattcggaac
     1921 tatgttggct tcgcacagca tcgcagtcga aaaccagacg gcaatggtat tgggtttcgg
     1981 aggttcggca aaagctgtcg ttctatactt attggataat ggcatcaata acattatcat
     2041 cgtttcacgg aaaaaaggcg ctctcacgga tattccggac gatccgcgca tctccttgat
     2101 gagttatgat gagataacgg acatttcagg cgatctgatc atcaacacga caccgctcgg
     2161 catgttccct gacaaaatcg gcatcagtgc ggtaccattg gaagtgatct gtcatttcag
     2221 tgcggcagta gatctgatct acaacccacn nnnnnnnnnt ttggcaaaaa cagctggcct
     2281 gaagacctgc aacggattgg aaatgttggt gcaccaggga atcaaatccc aggaaatctg
     2341 gctggaacgg cctttcgaac ttgcagtgaa ccaagaaatc tatgacaaaa tcacacaata
     2401 acgggaatag gtgaaggatt tgggaaaaaa tatcatcctg atcggaatgc ctggtgcagg
     2461 caagacgact ttaggtaaat tattaagcaa tataaccggt ttccgtttca tcgactcgga
     2521 tgccttcatt gaggagaaga ccggtgagag catcaccgac ctcttccaga ttggagagga
     2581 ctatttccgt aatatcgaat cggatgccta taaggaaatt gccgattccg aagatacgat
     2641 cgtgagcaca ggcggcggtg tcgtcaaacg cagtgaaaat atcgtggctt tgaaaaagag
     2701 cggaacagtc tattatatcg accgcgcagt cgaagacatc acgcatgaca tcgaagtttc
     2761 tcatcgtcca ctcttaaagg acggcctgga acggctccat gcgctgaagg ccgaacgcga
     2821 ggccctgtat caagacgctg cggatttcgt cgtcaagaac gacaaaccgt tggaagagat
     2881 cgccgctgaa attgttgcgt gccactttgg agtaggaaag gaaggatcag cccatgacaa
     2941 ccctcaataa ggaactaaac atcacgatcg tcggtctggg cgtaatcggc ggatctttcg
     3001 cgatggccct tcacgatgcc ggctaccgca atctttacgg ggtcgacatc gaccaagcga
     3061 cgctggacaa ggccaaagcg cagcgactca tcaaggaggg ctatcaggat ggcaaagaca
     3121 tcctaccgca atcggatttg gtgatttttt cgctctatcc ccgtctggtg cacgacttca
     3181 tcgttaccca taagcagtac ttcaagccgg gctcgattct aacggatgcg acgggtgtca
     3241 aaaagttctt catcgaagat atcctgtcgg ttttgcctga ctcgatcgac tttgtcttcg
     3301 gtcatccgat ggcaggacgt gaaaaaaaag gcatcgattt cgccagtagt tccgtctaca
     3361 aaaatgcgaa ttacatcttg acaccgctcc cttccaacaa agaggagaac cttgccttca
     3421 tggaagcttt gatcatggag atggggttcg ggaggatcac gcgcatctct gccgattacc
     3481 atgacgaaat gatagccttc accagccagt tgccgcacgc gatcgccgtg gcgttggtca
     3541 acagcgatgt cgaaggccgc gaaacggaga aatttaccgg cgacagttac cgcgaattga
     3601 cccggatcgc caaaatcaac gaagaactgt ggagtgaact ttttcttgag aacaaagagt
     3661 atttgctgaa gagcatccag acgttcgagg atcagctcga cctgatcaag gcggctatca
     3721 agaacgacga cagcgtcgcc ctgaaacagc ttttcatcca gtccacgaag cgacgggaaa
     3781 agttcgacag atgatggttt gctttcatta tacaagcaat gacaaaaagg atgccgtaat
     3841 ggtatccttt ttacttagca tccaagggct tcggcaagtt gcgtattgcc ctgaagtcga
     3901 ctaaaataaa agtagtgtat tgatatttcg aataaccgaa agaaggctga aaaaccatgc
     3961 gtgagaagct gaaaaaattc agttatttag tcatcctgtt catttttgcg agttttatcc
     4021 tgatcactgg atatgaattt gtaaggttcc tccaaacgag aggcacagaa aagcaaacgg
     4081 agcatttctt gcggctcgtt caagcaggct ttggattggt tgcgctgctc ttcccttcgc
     4141 tcctgcggaa gcatacccga atccttttac ctcaaagaat caccttcatc tatgccgtgt
     4201 ttctctattt ggcgctcttg ctgggcagtc ttggcgggtt ctatgacacg gttgccgaat
     4261 gggataccct ccagcatgcc ttgagttccg ctctgttcgc ggtgcttgga ttttccgtca
     4321 tcgccaacct gcaggagggc ggcatcgaaa gattgaatct gacaccggtt ttgtcttcgc
     4381 ttttttcttt ctgtctggct acgacactgg gagtgctctg ggaattctat gagtatttcg
     4441 ccgacagcgt catgggtcag aatacccaac ggactgccac agtcgatggt atccctctga
     4501 tcgggcaagc agctttgtcg gatacgatgg gtgacctgat cgtgaactgc agcgccgcag
     4561 caatcgtcgc tatcctgggc tacttcgcct tgaaaagcag cggcaattgg atggaatcgt
     4621 atctcatcac ctttgataca gaaccagaaa ataacaaacg aataaggggt aaaaaatgat
     4681 tactatacgg aatctttcca tagataatat cgaagacgcg cttgccttat ccgtcgatga
     4741 ttggcagcgc aaatacgtcc ggccgaacgc tgaaatgatc gcgttggcct atgtcggacc
     4801 gcaagaatta ttcccgatcg ccctatacgc gggggaagag ctggtcggct tggcgttcgt
     4861 caaaaaagaa caaaaggcgg cgatcatgac gctcgaacag attatgatcg gccacccttt
     4921 ccaatccaaa agtttcggtt ccgaaagtct ccgggaaatc gccaaatgga ttgaaacgca
     4981 attcagctgc tcgctgctgc aggcatccgt ccagattggt aaccaatggg gacgcgaaac
     5041 gcttgaaaac gcaggcttca tgaagcgcgc caccgatctc gaaaaacgcg aaatcgaaat
     5101 gatctatatc atcaaatgaa aacaatgggg gatacaaaat gaaacctgga caatacgaag
     5161 cttatatcaa atggcgcgac agtatggtcg atttttcgga ggacctcggc gaggacg
//
LOCUS       DAOMUJ010000074         5182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4430_length_5182_cov_2.042130, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000074 DAOMUJ010000000
VERSION     DAOMUJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_5182_cov_2.042130"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1215)
                     /locus_tag="PLT73_03030"
     CDS             complement(46..1215)
                     /locus_tag="PLT73_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRM19387.1"
                     /translation="MFEIKTYNAIAPEGLNLLNADQFVLNSGANPDGIILRSEKLHGM
                     EFPESLKAIARAGAGVNNVPVEECSEAGVVVFNTPGANANAVKELVLAAMIMAARPIK
                     EGLDWVQTLSGDDVEKQVEKGKSQFAGTEISGKTLGVIGLGAIGAMLANDAFRLGMEV
                     IGYDPYVSVETAWNINRRVQKAASIAEVLAKADYISIHVPVNAETKGMVDAAFLAQMK
                     DNAVLFNFARGELVNTADMLAALKEKSIRKYVTDFADEALLHNDDIIVMPHLGASTEE
                     AEVNCAIMAAQTLKYFLETGNIVNSVNFPGVDIAMHSPIRITVINKNIPNMVGQISSG
                     LAKYFINIEDILNRSRGNYAYTVIDIAETDQAVLDEVVENFKKIDGIVNVRVIRR"
     gene            complement(1215..2300)
                     /gene="serC"
                     /locus_tag="PLT73_03035"
     CDS             complement(1215..2300)
                     /gene="serC"
                     /locus_tag="PLT73_03035"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM19388.1"
                     /translation="MKRARNFSAGPAILPESVLEKVQSEMLSYKDSGMSVMEMSHRSS
                     LFEEIIQDAEQLLRELMNIPDGYKVLFLQGGASLQFSMIPMNLGKGGKVGYINSGSWA
                     KKAISEAKILKVPNIEVLASSEADNFTYIPEIPETDEAYDYIHITTNNTIEGTAFQTI
                     PRFVNAPIVADMSSNILSNRYDVNDFGLIYAGAQKNIGPAGLTIVIVKEDLIQTENAL
                     PSMLDYRTHIKNDSLYNTPPSFSIYVAKLVFEWLKDLGGVEAMVKMNEEKAGLLYRTI
                     EESDLFSSPVAVNSRSLTNIPFVTGDKELDAKFVKEATALGFLNLKGHRSVGGMRASI
                     YNAFPYEGVKELTEFMKAFEAKEGKFK"
     gene            complement(2625..3926)
                     /gene="eno"
                     /locus_tag="PLT73_03040"
     CDS             complement(2625..3926)
                     /gene="eno"
                     /locus_tag="PLT73_03040"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM19389.1"
                     /translation="MPYITDILAREVLDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EHEAIELRDEDKTRYLGKGVLKAVANVNDIIADAILGFDVRDQLAIDKTMIDLDGTPN
                     KEKLGANAILAVSIAVAHAAADYLDIPLYQYLGGFNAKTLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEALRWGAEIFHELKSILKKRGLETSVGDEGGFAPRFKGTEDG
                     VETILEAIKSVGLEPGKDVYLGFDCASSEFYENGVYNYAKFEGEGGAKRTAAEQVDYL
                     EELVNKYPIISIEDGMDENDWDGWKLLTERLGERVQLVGDDLFVTNTEILSNGIEKGI
                     GNSILIKVNQIGSLTETFEAIEMAKKAGYTAVISHRSGETEDATIADIAVATNAGQIK
                     TGSLSRTDRIAKYNQLLRIEDQLGELAVYDGLKSFYNLKNK"
     gene            complement(4021..>5182)
                     /gene="gpmI"
                     /locus_tag="PLT73_03045"
     CDS             complement(4021..>5182)
                     /gene="gpmI"
                     /locus_tag="PLT73_03045"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRM19390.1"
                     /translation="HINHVIALIKSAKEKGVKKLYLHAFLDGRDVDPHAAPGYIETVE
                     KAMEEIGLGEIATVSGRYYAMDRDNRWERVELAYNAIAHGEGEKFESAQDVVKASYAD
                     GKTDEFVLPTVIVKDGKPVGPLQDNDAIIFFNFRPDRAIQLSNAFTNEEWTNFDRKER
                     ATNVKFVTMTSYQDTVIADIAFKPIPMTNVLGEVLAQHNLSQLRIAETEKYPHVTFFM
                     NGGRHEPFEGEGRILIPSPKVATYDLKPEMSAYEVGEALVKEINDDKYDAIILNFANP
                     DMVGHSGMLEPTIKAIEAVDENLGAVVDAIHAKGGYAIIFADHGNADTMSTEDGQPHT
                     AHTTVPVPVIVTKEGVTLRTDGKLADVAPTMLDLLNVEKPAEMTGESLIVKA"
BASE COUNT         1294 a         1164 c         1115 g         1609 t
ORIGIN      
        1 tggtttctcc aacgtacggg tgtctaaacg cgaatcatca cactcttatc ttctgatgac
       61 acggacgttt acgatgccgt cgattttttt gaaattctcg acaacttcat ccagtactgc
      121 ttggtcagtt tcggcgatat cgatgacagt gtaggcatag ttgccgcggc tgcggttcag
      181 gatatcttcg atgttgatga agtatttagc caaaccggat gaaatctgtc cgaccatgtt
      241 cgggatgttc ttgttgatga ccgttatgcg gataggagaa tgcatcgcga tatcgactcc
      301 cgggaagttc acggaattca cgatgttccc tgtttccagg aagtatttta atgtttgggc
      361 tgccatgatt gcgcagttca cctcagcttc ttcagtagaa gcgcccaagt gaggcattac
      421 aatgatgtca tcattatgaa gcaaagcttc gtcagcgaaa tctgtaacat atttgcggat
      481 gctcttttct ttcaatgctg ccagcatatc agctgtgttg accaattcgc cacgggcaaa
      541 attaaaaaga acagcattgt ctttcatttg agccaggaaa gctgcatcaa ccataccttt
      601 tgtttcggca ttgacgggca catggatgga aatgtaatca gctttagcca atacttcagc
      661 gattgaagct gccttttgga cacgacggtt gatgttccat gctgtttcca ctgaaacata
      721 tgggtcataa ccaatgactt ccatgcctaa acggaatgca tcgttggcca acatcgcgcc
      781 gatagcacca aggccgatga cacccaatgt tttaccggag atttccgtcc ccgcaaattg
      841 gcttttgcct ttttcaactt gcttctcaac atcgtcacca cttaatgttt ggacccaatc
      901 cagtccttct ttgattggac gggcagccat gatcatagct gccaacacaa gctctttgac
      961 cgcattggca ttcgcgcctg gtgtgttgaa gacaacgacg cctgcttcgg aacattcttc
     1021 aacgggtacg ttgttgacgc cggcgccggc acgggcaatg gctttcaagc tctcagggaa
     1081 ttccatgcca tgcagttttt cactgcggag gatgatgccg tcaggatttg cgcctgaatt
     1141 aaggacaaat tgatctgcat tcagtaaatt taaaccttct ggggcaatcg cgttgtaagt
     1201 tttaatctcg aacattattt gaatttccct tccttagctt caaaagcttt cataaattct
     1261 gttaattctt tgacaccttc gtacgggaat gcgttgtaga tacttgcgcg cataccgccg
     1321 acagaacggt ggcctttcag gttaaggaag cctaacgctg tagcttcttt gacgaatttc
     1381 gcatccaatt ctttatctcc ggtaacgaat gggatattcg tcaatgatcg ggaattgaca
     1441 gcaactggag aagagaataa atctgattct tcaatcgtac ggtacagtaa gccagctttt
     1501 tcttcgttca tttttaccat agcttccacg ccgcccaaat ccttcaacca ttcgaagact
     1561 aatttagcta catagataga gaaacttggt ggtgtattgt atagagaatc gtttttgatg
     1621 tgtgtacggt aatcaagcat gcttggtaat gcattctcgg tttggatcag gtcttctttc
     1681 acgatcacaa tcgtcaatcc tgcaggcccg atattttttt gtgctccagc gtagatcaat
     1741 ccgaagtcgt tgacatcgta acgattggat aaaatgttgg aagacatatc ggcaacgatc
     1801 ggtgcattca cgaaacgagg gattgtttgg aatgctgttc cttcgattgt gttgttggtt
     1861 gtgatatgga tgtagtcata tgcttcgtct gtttccggaa tttcaggaat gtaagtgaaa
     1921 ttatccgctt cggaacttgc caatacttca atattgggca cttttaaaat cttagcttca
     1981 gagatggctt tcttagccca tgatccggag ttgatgtaac cgactttgcc gccttttcca
     2041 agattcatcg gaatcatgga gaactgcaga cttgcaccac cttgcaggaa caaaactttg
     2101 tatccatccg gaatattcat taattccctt aataattgtt ctgcatcctg aatgatttct
     2161 tcgaacaagg aggagcggtg actcatctcc ataactgaca tgccactatc tttgtaggat
     2221 agcatttcac tctgtacttt ctctaagact gattctggta atattgctgg accagcggaa
     2281 aaatttcttg ctcttttcat ccttttgtct cccccttatc aattgtcctc attatttatt
     2341 aataataatt taatcttatt atagcagatt atgcgacgaa tgaaagcgga aatagctaat
     2401 tttcaaaaaa atttacatgc agatgttagg aacggcaatt aaaaatatga tcatattgga
     2461 gtttgagtta attttattac ctaaaatccg aacgatacag gcataaacaa caaaaaaagt
     2521 ccggcgttcc aagcgccgga cttaatctcg tgtctggtca tcccagactg tttttatgtg
     2581 aaggcagtgt tccaagcaac tgacttcctg ttagtgaata aaaattattt gttttttaag
     2641 ttgtagaaag atttcaagcc atcgtataca gccaattcgc ctaattgatc ttcgatgcgt
     2701 aacaattggt tgtatttagc gatacggtca gtacggctca atgaaccagt tttgatttgt
     2761 ccagcgttag ttgcaacagc gatgtcagcg attgtagcat cttctgtttc gcctgaacgg
     2821 tgagaaataa ctgcagtgta accagctttt ttagccattt cgatagcttc gaaagtttca
     2881 gtcaatgaac cgatttggtt aactttgatc aggattgagt taccgattcc tttttcgata
     2941 ccgttagaca agatttcagt gttagttacg aacaagtcgt caccaactaa ttgaacgcgt
     3001 tcgcccaaac gttctgtcaa aagtttccag ccatcccagt cattttcatc cataccgtct
     3061 tcaattgaga tgattgggta tttgttgact aattcttcca agtaatctac ttgttctgca
     3121 gcagtgcgtt tcgctccgcc ttcaccttcg aatttagcat agttgtaaac gccgttttcg
     3181 tagaattcag atgatgcaca gtcgaatcct aggtatacat ctttgcctgg ttccaaacca
     3241 actgatttga ttgcttccaa aatagtttct acgccatctt cagttccttt gaagcgagga
     3301 gcaaatccgc cttcatcccc aacagaagtt tccaatccgc gttttttcag gattgatttc
     3361 aattcgtgga agatttcagc accccaacgc aatgcttctt tgaaagaagg cgcgccgact
     3421 ggcaagatca tgaactcttg gaaagcgata ggagcatctg agtgagatcc gccgttgatg
     3481 atgttcatca ttggtgttgg taaagtttta gcgttgaatc cgcctaggta ttggtataaa
     3541 ggaatatcca agtaatctgc tgcagcgtga gctactgcga tagatactgc caagattgcg
     3601 ttagcgccta atttttcttt gttaggagtg ccatctaagt cgatcattgt tttgtcgata
     3661 gctaattggt cacgtacgtc gaaacctaag atagcatcag caatgatgtc gtttacgttc
     3721 gctacagctt tcaaaacacc ttttccaagg taacgcgttt tgtcttcatc acgtaattcg
     3781 attgcttcgt gttcgccagt agaagctcct gatggaacca ttccgcgtcc gaaagcgccg
     3841 ctctctgtgt aaacttcaac ttcgatagtt gggtttccgc gtgaatctaa tacttctcta
     3901 gctaaaatat ctgtaatgta tggcatggtt ttctctcctt ttagttggct gaattacttg
     3961 tgttaccaag tatcatttta gacaattatc ttactttatg caatagctga agcgttttgc
     4021 ttacgcttta acgatcagcg attcgcctgt catttctgct ggtttttcta cgttcaacaa
     4081 atcaagcata gttggagcaa cgtcggccaa tttgccgtcg gtgcgcaacg taacgccttc
     4141 tttggttacg atcacaggaa ccggtacggt tgtgtgcgca gtatgcggtt gtccgtcttc
     4201 agtggacata gtatccgcgt tgccgtggtc agcgaagatg atggcatagc caccttttgc
     4261 atggatagcg tcaacgacag cacccaagtt ctcatccact gcttcaatcg ctttgattgt
     4321 aggttccaac atacctgaat ggcctaccat gtcagggttc gcaaagttaa gaataatggc
     4381 gtcgtatttg tcgtcattga tctctttcac taatgcttcg cccacttcat aagcactcat
     4441 ttcaggtttc aaatcatagg ttgcaacttt cggtgaaggg atcaggatac gtccttcgcc
     4501 ttcgaaaggc tcgtgacgtc cgccgttcat gaagaaagtt acatgcggat atttttcagt
     4561 ttccgcgata cgcagttgtg aaaggttgtg ttgtgccaac acttcaccca gcacattcgt
     4621 catcgggatc ggcttgaagg cgatatccgc aataactgtg tcttggtaag aagtcattgt
     4681 cacaaatttc acgtttgttg cgcgctcttt gcgatcgaaa ttcgtccact cttcgttagt
     4741 gaaggcattc gacaattgaa tcgcgcggtc cggacggaag ttgaagaaaa tgatcgcatc
     4801 attgtcttgc agcggtccta ctggcttgcc gtctttaacg attacagtcg gcagtacgaa
     4861 ctcatccgtt ttaccgtctg catagcttgc ttttacgaca tcttgtgcag actcaaactt
     4921 ctcgccttcg ccgtgtgcga tggcgttgta tgccaattcc acacgttccc aacggttgtc
     4981 acggtccatt gcgtagtagc gaccggaaac agtcgcaatt tcgcccagtc cgatctcttc
     5041 catcgctttt tccactgttt cgatgtagcc tggtgcagcg tgcggatcca cgtcgcgacc
     5101 atcaaggaaa gcatgcagat aaagtttttt gacgcctttt tctttagcgc tcttgatcaa
     5161 agcgatgaca tgattgatgt ga
//
LOCUS       DAOMUJ010000075         5118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4500_length_5118_cov_2.338929, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000075 DAOMUJ010000000
VERSION     DAOMUJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_5118_cov_2.338929"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLT73_03050"
     CDS             <1..267
                     /locus_tag="PLT73_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HRM19391.1"
                     /translation="GPDKPELSHWASTVTFKYVGAPMSDADRAVNPLGYQVTDYRTDP
                     ESLTAVERQAYKPPEPSVSPLASLSDLATEVAAPATPASAAITN"
     gene            276..>698
                     /locus_tag="PLT73_03055"
     CDS             276..>698
                     /locus_tag="PLT73_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HRM19392.1"
                     /translation="MKELLIACLSATLLCGAIAETIPEPGAADARIRVVTYNPRNVVR
                     LNTFFGVSTHIKFSETEQIKDVAVGDDLAWKVIPRGNNMFIKPTAKEGDTNITVVTNK
                     RIYQFVAVVLNEKNQKAAWANRDLIYSLSFRYSDDDDAN"
     assembly_gap    699..708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <709..1115
                     /locus_tag="PLT73_03060"
     CDS             <709..1115
                     /locus_tag="PLT73_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HRM19393.1"
                     /translation="KAEADKVKREDIKNRLTRAPSRTGHNMDYWVAGSQEISPNGAYD
                     DGRFVYLIFNNNAEMPAVYETDETGKESLVNTHVTSGNTIVLHRLAEKFTLRKGDFVA
                     SVWNKAYDGSGGANNLTGTVAPDVERVIKEVN"
     gene            1116..2402
                     /gene="virB10"
                     /locus_tag="PLT73_03065"
     CDS             1116..2402
                     /gene="virB10"
                     /locus_tag="PLT73_03065"
                     /inference="COORDINATES: protein motif:HMM:NF038091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HRM19394.1"
                     /translation="MSQDQDKHVAAGDDRKDAWRGQDKGFRGRENGQGQPSDDFDDDA
                     DLPSLNRKRGGNKLITAAGYVFMALLAVAAIVAVNRNPSAPVKKGDDGKVANRLPALI
                     VPAPPEPPASLAPKAEVAQANEEDDIWKRKMSGNLVAQGGSSGGGAGSRASGPMSRQQ
                     IEQERMAQLMGQGAGMHGMGQGGGASFIGPDGNPIDQAGPEGGIVTSSARADSLGQRL
                     KPTVTEAVSATVLPRRDYLLAKGATLDCALETAIDSSVPGMTTCRLTRDVYSDNGRVL
                     LLDRGTQLVGEYAGSLQRGQARMFLLWTRAKTPAGVVVSLNSPSTDSLGRSGISGYVD
                     NHFWERFGAAIMTSFLKDTVNIVANNRTKKPGDGGDQTNIYSGTVQSGEQIVSAMLRE
                     QSAIPSTLIVNQGQHIQVLMARDLDFSDVYGLRAVH"
     gene            2484..3461
                     /gene="virB11"
                     /locus_tag="PLT73_03070"
     CDS             2484..3461
                     /gene="virB11"
                     /locus_tag="PLT73_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HRM19395.1"
                     /translation="MLDTYLKPIYQHLQGDVTEVAINKPGEIWTEGPSGWVRHAAPEM
                     SYQSCMHIGQLIATYNEKDISIDRPILSAALPDGERVQVIIPPACLANTVSMTIRKPS
                     MTDKTLEELEASGAFSKVEDANDGLRPFETDLLELKKQRRIREFLHRVVLTKRNLLIV
                     GKTGSGKTTLTRSLIHSIPKTERLVTIEDVHELFMDDVPNKVHLLYAREDEGGSKVTA
                     KQSLASCLRMKPDRILLAELRGDEAWEFVKSINTGHPGSISTMHANGAWETFEQLTAF
                     IKDSRTGAHLETDDIKRRLYTTIDVVVYMDRWKVKEIFYDPEFKRSQMV"
     gene            3427..>5118
                     /locus_tag="PLT73_03075"
     CDS             3427..>5118
                     /locus_tag="PLT73_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM19396.1"
                     /translation="MTLNLSAHKWFKPALWIFVFIVITLAWLHLAGAVVLAGVGRDYN
                     KVNAYTFIQYAVYFGEQKRINAWLWSGAGFSTVALLLPLVLFLKPSRRSLHGDARFAS
                     AREIRKAGLTGNRGIIVGKKGSGYLMFDGSQHVLLSAPTRSGKGVSVVIPNLLTWPES
                     CVVLDIKRENWDITSGYRRKYGQRCYLFNPGASDGRTHRYNPLGYISEDPGKRIDDIQ
                     KIANMIFPDVQGTDPIWTATPRSLFLGVVLFLLESPGKPVTLGQVLRETLTDGDGKDY
                     FDKAAKDRRDCGNGLSGACVRGLQSYTSIASENTRSGIMTSFRSRLELWMNPAIDAAT
                     SENDFDLRDLRKRKMSIFIGITPDNLERMAPLINLFFQQLVDLNTRELPSQNPDLKYT
                     CLLLMDEFTSMGKVNILSKGISFIAGYGLRMMPIIQSPAQLTDVYGREAAETFRTNHA
                     LNIVFPPKASETESARDISEWLGYTTTKSASKSRNTEIFKRRNSSESVSDQRRALLLP
                     QEITSLGEEAELIVLENVLPIQAKKVRYFADAKFMDRLKEISPSLSAIGGRLPNKK"
BASE COUNT         1320 a         1295 c         1372 g         1121 t
ORIGIN      
        1 ggcccggata agccggaact ctcgcactgg gcatccacag tcacattcaa atatgtagga
       61 gcacctatgt ctgatgctga ccgggcggtt aatccgcttg gctatcaagt cacagactat
      121 cggactgacc ctgaatccct tacagccgtt gagcgccagg cctataaacc gcctgagcca
      181 agcgtgtcgc cattggcgtc tctctctgat ctggctaccg aagtggcggc gcctgcgact
      241 cccgcgagcg cagctatcac aaattgaggg attgaatgaa agaactactg atcgcttgcc
      301 tatcggcaac gctgctatgc ggtgcaattg cagaaacgat accggaacct ggggcggcgg
      361 acgctcgcat tcgggtcgtc acctacaacc caagaaatgt tgtgcgcttg aataccttct
      421 ttggggtttc aacgcatatc aaatttagcg aaactgaaca gatcaaagac gtggcagttg
      481 gtgatgatct tgcctggaaa gtcattccac gtggaaacaa tatgtttata aagcccacag
      541 caaaggaggg cgacaccaac attaccgtcg tcacgaataa gcgaatttat caatttgttg
      601 ccgtagtttt gaacgaaaaa aatcaaaagg cggcatgggc gaatcgcgat ctgatttatt
      661 ctctttcttt ccgttattca gacgacgacg atgcaaatnn nnnnnnnncc aaagcagaag
      721 cggataaagt aaaacgagaa gatattaaaa atcgactcac acgcgcaccg tcccgcacgg
      781 gtcacaacat ggattattgg gtggctggaa gtcaggagat cagcccgaat ggcgcatacg
      841 atgacggtcg ctttgtttat ctaattttca acaacaatgc ggaaatgcct gcagtttacg
      901 aaaccgatga aaccggcaag gaatccttgg taaacaccca tgtgactagc ggaaatacga
      961 tcgtgttgca tcgactggcc gagaagttca cgcttcggaa aggtgatttt gtggctagcg
     1021 tttggaataa agcctatgac ggtagcggag gggcaaataa tttaacgggg actgtcgctc
     1081 ctgatgtgga acgtgtaatc aaggaggtca actaaatgag ccaggatcaa gacaagcatg
     1141 tggccgctgg cgatgaccgc aaggatgcgt ggcgcggtca ggacaagggg tttcgcgggc
     1201 gtgaaaacgg ccagggacag ccatctgatg actttgatga cgatgctgat ctgccgtcgc
     1261 tcaaccgaaa gcgtggcggt aacaagctca ttacagcggc aggatatgta ttcatggctt
     1321 tgctcgctgt cgcggcgatt gtggccgtga atcgaaaccc gtcggctcca gtcaaaaagg
     1381 gggatgacgg caaggttgcg aaccgcttgc cagcactcat cgtaccagcg cctcctgaac
     1441 ccccggcatc gcttgcacca aaagcagagg tagcccaagc gaacgaggaa gacgatattt
     1501 ggaaacgcaa aatgtctggc aatctggtgg ctcaaggcgg cagctcaggt ggcggcgcgg
     1561 gctcaagggc ttcggggccg atgagcagac agcagattga acaagagcgc atggcccagt
     1621 tgatggggca gggcgccggg atgcatggga tgggtcaagg gggcggcgca tcgttcatag
     1681 gccctgatgg caaccccatt gatcaagcag gccccgaggg cggcattgtg acgtccagcg
     1741 cccgcgcgga ctcccttggg cagcgcctca aacccacggt caccgaagcc gtttcagcca
     1801 ctgtgcttcc gcgtcgtgac tacctgcttg caaagggcgc aactctcgat tgcgccctgg
     1861 aaacggccat tgattccagc gttccaggga tgacaacttg ccgcctgaca cgggacgtgt
     1921 attccgacaa cggccgtgtt ctcttactgg atcgcggcac ccaactggtc ggtgaatacg
     1981 ccggatctct gcaacgtggc caggcccgca tgttcctgct ttggacgagg gcaaaaacgc
     2041 ccgctggtgt ggtggtcagc ctcaactctc cgagcactga ttctttgggc cgtagcggta
     2101 tcagtggcta tgtggacaac cacttttggg aacgatttgg cgcggcgatc atgaccagct
     2161 tcctcaagga cacggtgaac atcgttgcca acaaccgaac caaaaaacca ggcgatggcg
     2221 gtgatcagac caacatctat agcggcacgg ttcaatccgg ggagcagatc gtcagcgcaa
     2281 tgctgcgcga acagtcggca attccatcga ccttgattgt caaccaaggt cagcacattc
     2341 aagtgctcat ggcgcgcgac ttggatttca gcgacgtgta cggcctgcga gctgttcact
     2401 agcccatgct cgactcatac aacacgagcg cagcggtcgc gcagcccacg ccccatacag
     2461 gggaaaggtt ggtgcgccgg gccatgctcg acacgtacct gaagcccatc taccaacact
     2521 tacagggtga cgtcaccgaa gttgcaatca acaagccggg tgagatttgg acagaagggc
     2581 cttcgggctg ggtgcggcat gcagcacctg aaatgtcgta ccaaagttgc atgcacattg
     2641 ggcaactgat agcgacctac aacgaaaaag acatttcgat tgatcgaccg attttgtctg
     2701 ccgccttgcc cgatggggaa cgggttcaag tcattattcc tccggcgtgt cttgcgaata
     2761 cggtgtccat gacgatccgc aagccaagca tgaccgacaa aactttagag gagctggaag
     2821 caagcggcgc attctcgaaa gtcgaagatg caaacgacgg gcttcgtccg tttgagacag
     2881 atttattgga attaaagaag caacgtcgca tacgggaatt cctgcatcgt gtggttttga
     2941 ctaagcgcaa tttgctcatt gttggcaaaa ctggttctgg aaaaaccaca ttaacgcgca
     3001 gtctaattca ctccattcct aaaactgagc gactggttac tatcgaagat gtgcatgaat
     3061 tgtttatgga cgatgtgcca aacaaagtgc acttgcttta cgcccgtgaa gatgaaggcg
     3121 gatcaaaagt aaccgccaaa caatcactag cttcgtgtct gcggatgaaa cccgatagga
     3181 ttttactcgc tgaattacgt ggcgatgaag catgggaatt cgtgaagtcg atcaacactg
     3241 ggcacccagg cagcattagc accatgcacg caaacggtgc gtgggaaacc tttgaacaat
     3301 tgacggcttt catcaaggat tcccgcactg gcgcccacct cgaaactgac gacattaaga
     3361 gacgccttta cacgacgatt gacgttgtgg tgtatatgga ccgctggaaa gtcaaagaaa
     3421 tattctatga ccctgaattt aagcgctcac aaatggttta aaccggcact atggatattt
     3481 gtattcatag ttataacttt ggcatggctg catcttgcag gcgccgtggt tctggctggc
     3541 gttgggagag attacaacaa agttaatgcg tatacattta tacaatatgc ggtgtatttc
     3601 ggcgagcaaa agcgaattaa tgcatggcta tggtcaggag ctggatttag caccgtcgcg
     3661 ctgcttctac cactggtact atttcttaag ccctcacgac gaagcctgca cggagatgct
     3721 cgtttcgcat cggcccgaga aattcggaaa gcagggttaa cgggaaatag gggaatcatc
     3781 gtaggcaaaa aaggctccgg ctacttgatg ttcgatggct cacaacatgt tttgctctct
     3841 gcgccaactc gctcaggaaa gggggtttcg gtcgtcatcc cgaaccttct cacttggcct
     3901 gaaagctgtg tggttctgga cataaagcgg gaaaactggg atatcaccag cggctatcga
     3961 cgcaagtacg ggcagcgctg ctacttgttc aaccctgggg caagcgatgg tcgaacgcat
     4021 cgatataacc ctttgggcta catcagcgaa gaccccggta aacgcatcga cgatatccag
     4081 aagatcgcaa acatgatttt tccggatgtg cagggcactg acccgatttg gacagcaact
     4141 ccgcgcagtc tgtttcttgg ggtcgttctg ttcttgctgg agtcacctgg aaagcccgta
     4201 acgctgggcc aggtgcttcg agagacattg accgatggcg atggaaagga ctactttgac
     4261 aaggctgcga aagaccgcag ggattgcggc aatggactgt cgggtgcatg cgttcgtggc
     4321 ctgcaaagct atacatccat cgccagcgag aacacgcgca gcgggatcat gacctcgttc
     4381 cggtcgaggc ttgaactgtg gatgaacccg gcgattgacg ctgccaccag cgaaaacgat
     4441 ttcgacttgc gagaccttcg caagcgcaag atgtcaattt tcattggcat cacccctgac
     4501 aacctagagc gtatggcacc gctcatcaat ctgttctttc aacagcttgt agatttaaat
     4561 actcgggaac tcccctcaca aaatcccgat ttgaaatata cgtgccttct cttgatggac
     4621 gaattcacgt ccatgggcaa agtcaacatt ttgagtaagg gaattagctt tattgcaggc
     4681 tacggattgc gaatgatgcc catcattcaa agcccggcgc aactcacgga cgtttatggc
     4741 cgcgaagccg ctgaaacttt ccgcaccaat cacgccctca acattgtatt tccgccaaaa
     4801 gcatccgaaa ccgaatctgc acgcgatatt tccgaatggt taggctatac aactactaag
     4861 agcgcatcga agtcccgaaa cactgaaata ttcaagcgcc gcaattcttc ggagagcgtc
     4921 agcgatcaaa ggcgcgctct actgctgccg caggaaatca cttctcttgg agaagaggct
     4981 gaattgatcg ttttggaaaa cgtcctccct attcaagcca aaaaggttcg atatttcgcg
     5041 gacgcgaaat tcatggatcg actcaaggaa atttcaccat cattgagtgc tattggggga
     5101 agattgccca ataagaaa
//
LOCUS       DAOMUJ010000076         5093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4526_length_5093_cov_1.814609, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000076 DAOMUJ010000000
VERSION     DAOMUJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_5093_cov_1.814609"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLT73_03080"
     CDS             <1..249
                     /locus_tag="PLT73_03080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758230.1"
                     /note="catalyzes the interconversion of D-ribulose
                     5-phosphate to xylulose 5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM19397.1"
                     /translation="NPGFGGQSFIESTLGKIEELATLREKHGYHYEIEVDGGITHETI
                     VKCIKAGADVFVAGSYIYDDENPSAAISRLKDACHNAV"
     gene            239..901
                     /locus_tag="PLT73_03085"
     CDS             239..901
                     /locus_tag="PLT73_03085"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRM19398.1"
                     /translation="MQSEKVVIVVGGDLTDVAALVRRYATSHRMVGADRGALKLAELL
                     PHFDAAIGDFDSVTPSEMEDIKKKSLVCRVLPAQKDETDTEAAIAYAIEMFNPEEIIL
                     LGAFGGRLDHLMSNLWLAFHPLVKQMTGKVSLMDRHNTLRFYLPGNYVLKKEEGKKYL
                     SFIGMTPIKELKLTGVAYPLNGKDYEYPIALVSNEFKDTEMTFSFGSGLLAVIQSSDK
                     RE"
     gene            complement(1005..1193)
                     /gene="rpmB"
                     /locus_tag="PLT73_03090"
     CDS             complement(1005..1193)
                     /gene="rpmB"
                     /locus_tag="PLT73_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRM19399.1"
                     /translation="MSKECFVTGRKSRSGNNRSHAMNYTKRTFKPNLQKVRILVDGKP
                     KKVWVSARALKSGKVERV"
     assembly_gap    1284..1293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1549..1911
                     /locus_tag="PLT73_03095"
     CDS             1549..1911
                     /locus_tag="PLT73_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM19400.1"
                     /translation="MAVKIQTQYGMVDLSNDVIATVVGGAATDNYGVVGMASKNQIRD
                     NISEILKKENYSRGVVIRQEDNGVAVDVYIIVLYGTKISEICRNVQTQVKYNLEMMLG
                     FSANTVNVFVQGVRVLKD"
     gene            1957..3627
                     /locus_tag="PLT73_03100"
     CDS             1957..3627
                     /locus_tag="PLT73_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM19401.1"
                     /translation="MKRTELTAEDFKAMVAIGAERLSENAEYVNSLNVFPVPDGDTGT
                     NMNLSFTSGAERVRLSNAATIDQLGADLAKGLLMGARGNSGVILSQLFRGFSKAIEGK
                     KTLSTIDFAEAFSHGVETAYKAVMKPVEGTILTVARESAKAGMKKAEHTDDIIELMGT
                     VLKVAQIALDKTPDLLPILKEVGVVDSGGQGLVYIYEGFMESLTGEKVKSAEKVVSQA
                     DVTEIAHQENYHNVSHAIASEDIQFGYCTEIMVKLGEGETVTDTFDYDTFRNHLNELG
                     DSLLVVADEEIVKVHVHTEYPGEVMNYGQKFGSLMKIKVDNMRMQHDAVLEKADTPAK
                     KLEKIPYGVIAVAAGEGVHALFKSMGVTTIINGGQTMNPSTEDILKAIESTNAEKTII
                     LPNNKNIFMAADQAAEVSDADVVVVASKTISQGLTAMLAFNESEDLETNKANMTAELE
                     NVVSGQITNAVRDTVIDGLAITKDDFMGIVDGKILTADKDRKAATIATLKKMITDDCE
                     IVTIIFGADSDENESKEIAAVIEAEYDFLEVEVQEGNQPVYSYLLSVE"
     gene            3726..>5093
                     /locus_tag="PLT73_03105"
     CDS             3726..>5093
                     /locus_tag="PLT73_03105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRM19402.1"
                     /translation="MEAEDNKQLDIYDPVGELPHVGPKRVEALHSLGIDTIYDLITHF
                     PFRYEDIKVKTLDEIEDSDKVTLKGQVVTAPVVHHYGFKKSRLSFKLAIEDAIISVTF
                     FNQPYLKQKIMLEDEVAIFGKWERARQTLAGMKILGSSNGQDDYAAIYHVNKTIKQQT
                     ILNLIKQAIELYSSVIPEILPQHLQQTYHLMTHREAIRAMHFPKNDEESEQARRQIIY
                     QEFFLYQVRLQMLQRSRKTNNRGIPILYDVDKLKGFIATLPFELTDGQKKVVNEICRD
                     LRKPYEMNRLLQGDVGSGKTIVAVIALMATVLGGYQGAFMVPTEILAEQHYRTLQSLF
                     EYTDVSVALLTGSTKAKDRKELLNRLASGDLHILIGTHALIQEDVVFADLGLVITDEQ
                     HRFGVQQRHKLNEKGNNPNVLYMTATPIPRTLAITTMGEMDVSVLDQMPDGRKPIKTI
                     WNKSDK"
BASE COUNT         1542 a         1042 c         1246 g         1253 t
ORIGIN      
        1 aacccgggct tcggtggcca aagcttcatc gaaagcacct tgggtaaaat tgaagagtta
       61 gctaccttgc gcgaaaaaca tggctatcat tacgaaatcg aagtcgacgg cgggattacc
      121 cacgagacga tcgtcaaatg catcaaagca ggggctgatg tattcgtagc gggttcttac
      181 atctatgacg atgaaaatcc atccgctgcc atcagtagac tgaaggatgc ctgccacaat
      241 gcagtctgag aaagttgtga tcgtggtcgg tggtgacctg acggatgtcg cagctttggt
      301 ccgccgttat gcgacaagtc atcggatggt gggcgcagac cgcggagctt tgaagcttgc
      361 cgaactgctc ccgcattttg atgcggcaat cggtgatttt gattctgtca ccccttcaga
      421 aatggaagac atcaaaaaga aaagtcttgt ctgccgtgta ctcccggcac aaaaagatga
      481 gacggatact gaggcggcta ttgcttatgc gattgaaatg ttcaatccgg aggaaatcat
      541 tcttttggga gctttcggag ggcgtctgga ccatctgatg agtaatttgt ggttggcatt
      601 ccatccactt gtgaaacaga tgacgggtaa ggtcagttta atggaccgcc ataatacgct
      661 gcgattttat ctgccgggta attatgtgct aaagaaagaa gaggggaaaa aatacttgtc
      721 gttcatcggc atgactccga tcaaagaact gaagctgact ggagttgcct atccgttgaa
      781 cggaaaagat tacgagtacc cgatagcttt ggtcagtaat gaattcaagg acacagagat
      841 gacgttttct tttggatctg gcctattggc ggtcatccaa agctctgata agcgcgaata
      901 aggaaagtgg ccacaacaat tgcggccact ttccttaatt ttaggatggg aaataaaaaa
      961 agatgagctg gaatcatgac atggtcccgg gctcatctat atttttatac tctttcaact
     1021 ttaccagatt tcaaagctcg agcagaaacc caaacttttt taggttttcc atcgactaag
     1081 atacgaactt tttgtaagtt tggtttgaaa gtacgttttg tgtagttcat agcgtgggaa
     1141 cggttattac ctgaacggct tttacgtcct gttacgaaac attcttttga cattttcttt
     1201 cctcctttgc tgttacagaa gcaaagcttc tttccaattc atacacttga ttaatttatc
     1261 ataatttaaa agtgaatgca actnnnnnnn nnnattgatt ctttaaagga aaaatacttg
     1321 acagctgttt ttttaatgat gtcatcacca aatgtctgta ctatgttgtc attgaaaagg
     1381 cactagtgct ggataagaaa actggaaaac agtgggaaat caataagaat tgccgcaaat
     1441 cgaccgtaaa tgagaaattt aatcacaaaa agttatttct tttgtataaa atatgctatc
     1501 ataatttata attacgattg tgtgcataca ttaaggaggc tattataaat ggcagttaaa
     1561 atccaaacac aatatggaat ggttgactta tcgaacgacg tgatcgctac ggtagtgggc
     1621 ggagccgcga cagataatta tggagttgtc ggcatggcga gtaaaaatca aatcagagat
     1681 aatatcagtg aaatattgaa aaaagagaac tactcacgcg gtgtcgttat tcgtcaagaa
     1741 gataatggag ttgctgttga tgtttatatc attgtactct acggcactaa aatttcagaa
     1801 atttgccgga atgttcaaac gcaggtcaag tacaaccttg aaatgatgtt gggattttcc
     1861 gcaaatacag tgaatgtgtt cgtccaaggc gtaagagttt taaaggacta aagattacag
     1921 atcaccattt gttatatggt gaaggaggat tataaggtga agagaactga attaacagcg
     1981 gaagacttta aggcgatggt cgcaatcggc gccgaacgcc ttagcgagaa tgcagaatat
     2041 gtgaattctt tgaatgtgtt ccctgttccg gacggagaca caggcacaaa catgaatcta
     2101 tcattcacat ctggagctga acgcgtaagg ctgtcgaatg ctgcgacaat tgaccaatta
     2161 ggagcggact tggctaaagg tttattaatg ggggcgagag ggaactcagg cgtcatttta
     2221 tctcagctat tccgaggatt ttcaaaagcg atcgaaggca aaaaaacttt gagtacaatt
     2281 gattttgcgg aggccttttc ccacggcgtc gagacagctt ataaagcggt catgaaacct
     2341 gtggaaggca ccattttgac ggttgcgcgc gaatccgcaa aagccggtat gaaaaaagca
     2401 gaacatacgg atgacatcat cgaattgatg ggaacagtcc tcaaggtggc gcagattgcc
     2461 ttggataaga cacccgatct gttgcctata ctgaaagaag taggcgtggt cgacagtggc
     2521 ggacaagggc tggtgtacat ttacgaagga tttatggaat cgttgaccgg agaaaaagtg
     2581 aagtcagctg aaaaggttgt ctcacaagcg gatgtcaccg aaatcgctca ccaagaaaat
     2641 tatcataacg tttcgcacgc aattgcgtca gaagatattc aattcggtta ttgtacagaa
     2701 atcatggtca aattaggcga aggtgaaacc gttacggaca cttttgatta tgacacattc
     2761 cgtaatcatc tgaacgaact tggagattcc ttattggtcg tagcagacga agaaatcgtg
     2821 aaggtccatg tacataccga ataccctggg gaagtcatga actacggcca aaaattcggc
     2881 tcactgatga aaataaaagt ggacaatatg cgcatgcaac atgatgcagt ccttgaaaaa
     2941 gccgatacgc ctgccaaaaa acttgaaaaa attccttatg gcgtgatagc tgttgctgcc
     3001 ggcgaaggtg tgcatgccct gttcaagagc atgggtgtca ccacaatcat caatggcgga
     3061 caaacgatga acccaagcac ggaagatatc ctcaaggcga tcgaaagtac aaatgccgaa
     3121 aaaacaatca ttttgccgaa caataaaaat attttcatgg ctgcagatca ggctgccgaa
     3181 gtttctgatg cggatgttgt agtagtggct tcaaaaacga tctcgcaagg tctgacagct
     3241 atgcttgcct tcaatgaatc agaagacttg gaaaccaata aagccaacat gactgccgaa
     3301 ctggaaaatg ttgtcagcgg gcagatcacg aatgccgtgc gcgacacagt catagatgga
     3361 ttggccatca ccaaggacga cttcatggga atcgtcgacg ggaaaatcct gacggccgac
     3421 aaggacagaa aagccgcaac gatagcaacc ctgaaaaaaa tgatcacgga tgactgcgaa
     3481 atcgtgacca tcattttcgg ggcagacagc gatgagaacg aaagcaaaga gattgcagcg
     3541 gtcatcgaag cggaatatga cttcttggaa gtcgaagtgc aagaaggaaa tcaaccggta
     3601 tacagttatc ttctttctgt agaataatct gaaaaagctg aacgagtttc tctgttcggc
     3661 ttttcttttt caaaaaacgg ttgatctgag aaatgtcctg ctcccagaag aaaggaggcg
     3721 aacggatgga agcggaagac aacaagcaat tggatattta tgatccagtc ggggagttgc
     3781 cccatgtagg accgaagagg gttgaggcgc tacattcgtt gggcattgat acgatctatg
     3841 atctgatcac acactttcct ttccgttatg aggacataaa agtcaaaaca ttggacgaaa
     3901 tcgaagattc ggacaaagtg acgctgaaag gacaagtcgt gacggctccg gtcgtccacc
     3961 attacggttt taaaaaaagc aggctatcat ttaaattagc catcgaagat gccatcatat
     4021 cggttacctt ctttaatcag ccttacctga aacaaaaaat catgcttgag gatgaggttg
     4081 cgattttcgg taaatgggaa cgcgcacgcc aaactttggc agggatgaag atcctcggca
     4141 gttcgaacgg acaggatgat tatgccgcca tttatcatgt caacaagacg atcaaacagc
     4201 agaccatcct gaatctgatc aaacaggcga tcgaacttta ttcctcggtc attcccgaga
     4261 tacttccgca gcatttgcag cagacttatc acctgatgac ccacagggaa gccatccggg
     4321 cgatgcactt tcccaaaaat gatgaagaat ccgagcaagc gagacggcaa atcatctatc
     4381 aagagttttt cctttaccaa gtgcgcctgc agatgctgca gcggagccgc aaaacgaata
     4441 accgaggcat tccgatcctt tatgacgtgg acaagctcaa gggattcata gcgactttgc
     4501 cgttcgaact tacggatggg caaaagaagg tcgtcaacga aatatgccgg gatctgcgca
     4561 aaccttatga aatgaaccgt ctgttgcaag gggatgtcgg cagcggcaag acgatcgtag
     4621 cggtaatcgc cctgatggct accgtcctgg gcggatacca aggggctttc atggttccca
     4681 ctgagatttt ggcggaacag cattatcgca ccttgcagag tctgttcgaa tatactgatg
     4741 tctccgtcgc cctgcttacc ggcagcacga aggcaaaaga ccgaaaagaa ttgttgaacc
     4801 ggcttgcatc aggggacttg catatcctca tcgggacgca cgcactgatc caagaagatg
     4861 tcgtttttgc cgacctgggc ctggtcatca ccgatgagca gcaccgtttc ggtgtccagc
     4921 aaaggcacaa gctgaatgaa aaagggaaca acccgaatgt tttgtatatg actgctacgc
     4981 cgatcccgag gacgttggcg ataaccacga tgggcgaaat ggatgtttca gtgcttgacc
     5041 agatgcctga cggcagaaaa ccgatcaaaa cgatctggaa caaatcggac aag
//
LOCUS       DAOMUJ010000077         5062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4569_length_5062_cov_2.589774, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000077 DAOMUJ010000000
VERSION     DAOMUJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_5062_cov_2.589774"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..911)
                     /locus_tag="PLT73_03110"
     CDS             complement(36..911)
                     /locus_tag="PLT73_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM19403.1"
                     /translation="MAENYIQKLLKPHYPGFSATETKIADYFVSLGMNIVNKTLANLS
                     EETKFSEATIFKFVKKIGFKGFQDFKISVASNYPTAEERTRQLAVFTDIKPSDTPTDI
                     SKKVIHSSQIMLQSLMSTLSGETLQQALDIIIHSKALHFFGLGASAVTALDAYHKFLR
                     TAIPVNYVSDYHMQLMHTSKLTENDCVFLFSHSGKTAETIRLAEVAAQNNAKIICLTG
                     NPPGILNQLSDVSIVVETEESIFQSEALSARLLYLTIVDIIYLSYIYHDEHTNLRSID
                     TIRQVLSTTKKEVDR"
     gene            1169..2173
                     /locus_tag="PLT73_03115"
     CDS             1169..2173
                     /locus_tag="PLT73_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HRM19404.1"
                     /translation="MTEKNKIAIVNSSSFGKMFDAHLARVGKLGPVDRFDFDGEIPGK
                     ELAEALQGYNIIIASVTPFFTREFFEHKDELKLIARHGIGFNNVDLEAAKEHGTVVTI
                     VPALIERDAVAENNVTNLLALMRRTIESAAEVKKDRWENRAKFIGNGLCGKTVGVIGV
                     GNIGSRVVEILHYGFRCEVLGVDPNKTALEIDIFGGKKVELDELLERAEVICLCASLN
                     DTNYHLISKETIAKMKDGIYISNSARGALVEESDIIEAIESGKLRGYATDVLEVEPGR
                     SDHPFLDFANIIVTPHTSAYTMECLEGMGDKCVSDCEAIVEGRMPQRAVQPISPYITK
                     "
     gene            2189..3292
                     /locus_tag="PLT73_03120"
     CDS             2189..3292
                     /locus_tag="PLT73_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008380572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRM19405.1"
                     /translation="MNTAVNVKVGPQFYKYGQGAMEVIPDILNEYGVKRVLLVHGTVS
                     WEKARPYLDFLDEEFTIQYEKYNGECSYNEANRLAKIVEEGNFDFIIGVGGGKLCDLV
                     YYVGALAKKPFGVVPTLASNCAPWAPLSVMYKDNGLAEGKTEHYKRQAAFLITDPALV
                     VDAPIKFFIAGLADTLAKWYESDMILEQPQFEANNFLMLARHSARICKDVIAEDGLKA
                     IEDMRTGVASAEFIKVSEIIFGVAGLVGGFGDKYARNTAAHAIHDAISAYLPGVHDYL
                     HGEKVAYGIFYQLALEGKWQVIDELIPMYESLHLPKSLTEMGEYPLSEEALDNIVALI
                     NKKEKVHLLPIPINETILKKAIIDLEKYINMEV"
     gene            3296..4120
                     /locus_tag="PLT73_03125"
     CDS             3296..4120
                     /locus_tag="PLT73_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM19406.1"
                     /translation="MENITAIQSLLIALWVGAVMSRAFLGGATLTLRFSPLMTGLIAG
                     IVMGDVQQAMIITAAIQLIYMGVFSPGGTMPSEPAIAAAIAVPVALMGNLQPEAAIAV
                     AVPVGLLGAYLYQFRFFLNTFLGKYTDKAVEDLNDGGIVRSIILYPTIASFLLFVPLV
                     FIALYFGAPVIADVITALEGTVVFHILTVVGGGLAAIGIATTIYVIGRKDYMVFFLLA
                     YLMSVVLASLSITMVTYAIIGALIAAIFVLAKGQGAKAAPASAGSASLDDDDDDDF"
     gene            4152..5021
                     /locus_tag="PLT73_03130"
     CDS             4152..5021
                     /locus_tag="PLT73_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM19407.1"
                     /translation="MINANLDAKEKNILAPEEITTKDVTKTYLRWHFANEIPHSFDRY
                     LAPSLLYAMMPILRKLYKDEDQLRAAYKRQLLFFNTQLSWGGGVITGLMSSMEQERAK
                     EVVNGEEVTMTDDLMYNTKAGLMGALAGIGDSIDSGTVQYIFIAIAVPWAQMGSPIGA
                     LFPFVAFALYQVLLGVFFARSAFKTGKNATGVMHSAGIQTVIEMLSILGMFMMGILAG
                     NYVKVSSSLEFAISGRPFVLQEMLDKIMPGMLPLAVVLGVYFYYIKKGLKVTRALVGL
                     TLILIVLAGIGIL"
BASE COUNT         1409 a          999 c         1297 g         1357 t
ORIGIN      
        1 gccgtccata tcgcaaacga ttgctttgat gggcattatc tatccacttc cttttttgtt
       61 gtcgaaagaa cctggcgtat cgtatcaatg gagcggagat ttgtgtgctc gtcatgatag
      121 atataggaca ggtaaatgat atcaacaatc gtgaggtaaa gcaaccgagc tgaaagagct
      181 tcggactgaa aaatggattc ttccgtttcc actacgatgc ttacatcact caattgattc
      241 aaaatgcctg gcggattccc ggtcagacaa attattttcg cgttgttttg ggcagccact
      301 tcggccagcc tgattgtttc ggctgtcttg cccgagtgtg aaaaaaggaa gacgcaatca
      361 ttctccgtca atttggaagt gtgcatcagt tgcatgtgat aatctgaaac ataatttaca
      421 gggatagccg tccgcaagaa tttgtgatag gcatccaaag cagtgacggc agaggcgccc
      481 aggccaaaaa agtggagtgc ttttgaatgg atgatgatat caagcgcttg ttgcagagtt
      541 tcgccagaca gtgtgctcat aagactttgc agcatgatct gagaggaatg aatcactttt
      601 ttagaaatat ccgttggcgt atccgaaggc ttgatatccg taaaaactgc caattgacgg
      661 gtgcgctctt cagccgtagg gtagtttgaa gcaacactga ttttgaaatc ctgaaatcct
      721 ttgaagccga ttttttttac gaatttaaaa attgtcgctt cggaaaattt tgtttcctcg
      781 gaaaggttgg ctaatgtttt gttcactatg ttcatgccca aactcacaaa atagtcggca
      841 atttttgttt cggtcgctga aaaacccgga taatgcggct ttaagagttt ttggatataa
      901 ttttctgcca tgatgagcac ctcctttttc tatagaatag cataaataca aaaataaata
      961 aataaataaa tattattttt taagccttta caaagtaaaa attatttact ataatcagag
     1021 tgtaaagaaa gaaaccgatc acagtcgagg gtttgaactg agcatcggta tttgaggttg
     1081 gtaagatgag tcttaatatt gtgtgggtac ttcgatccgg aatggatttg ttggacataa
     1141 agtcttgcga gtggaggagc aaaaaaggat gactgaaaaa aacaaaatcg caatcgtaaa
     1201 ttcaagcagc ttcgggaaaa tgtttgacgc gcatctggca agggtgggaa aattgggacc
     1261 agtcgatcgt ttcgatttcg acggcgagat accgggaaag gaactggcgg aggctctgca
     1321 aggatacaac atcatcatcg cgagtgtgac gccattcttc acaagggaat ttttcgaaca
     1381 taaagatgag ctgaagctga tcgcgcgtca cggaatcggg ttcaacaatg tcgatctgga
     1441 agctgccaaa gagcatggaa ccgtagtgac gatcgttccg gcattgatag agcgcgatgc
     1501 tgtagccgaa aacaatgtca ccaacttgct ggcactgatg cggcgaacga tcgagtcggc
     1561 agcggaagtc aagaaagacc gctgggaaaa ccgggctaag ttcatcggca acggtctttg
     1621 cggcaaaacg gtcggcgtaa tcggtgtcgg taatatcggt agccgagtag ttgaaattct
     1681 acattatgga ttcagatgtg aagtgctggg agtcgatccg aacaagaccg cgttggaaat
     1741 cgacattttc ggcggtaaga aagtcgagtt ggatgaactg ctggagcggg ccgaggtcat
     1801 ttgcctctgt gcaagcctga atgacacgaa ttaccacctt atttcaaaag aaaccatcgc
     1861 gaaaatgaag gacggtatct acatctccaa ctcagctcgc ggggcgcttg ttgaagaatc
     1921 ggacatcatc gaagcgatcg agagcggaaa actgcgcgga tacgcgaccg atgttctgga
     1981 ggtggagccg ggccgtagtg accatccgtt cctggatttt gcgaacatca ttgtgacacc
     2041 gcatacatct gcttatacga tggaatgctt ggaggggatg ggcgacaaat gtgtttccga
     2101 ttgtgaagcc atcgtggaag ggaggatgcc gcaacgagca gtgcaaccga tcagtcctta
     2161 catcacaaaa tgagaggtgg aacgatagat gaatacagca gtaaatgtca aagtcggccc
     2221 gcaattttat aaatacgggc agggtgccat ggaagtgatc ccggacatac tgaatgaata
     2281 cggtgtgaaa cgggtgttgt tggtccacgg aacggtttca tgggagaagg caagaccgta
     2341 tctggatttt ttggatgagg agtttacaat ccaatacgaa aaatacaacg gggaatgcag
     2401 ctacaatgaa gcaaacaggt tggcaaagat agtcgaagag gggaactttg atttcatcat
     2461 cggtgtcggt ggaggaaagt tatgcgattt agtgtactac gttggagcgc ttgcgaaaaa
     2521 accttttggg gtcgttccga cattggcgag caattgtgcg ccatgggcac cgttatctgt
     2581 catgtacaaa gacaacgggc ttgctgaagg caaaacagaa cattataaaa gacaagcagc
     2641 ctttctgatc actgatcctg cgctggttgt cgatgccccg atcaaattct ttatcgccgg
     2701 acttgccgat accttagcga agtggtacga atccgacatg atattggaac agccgcagtt
     2761 tgaagcgaat aattttttga tgcttgcccg gcattcggca aggatttgca aagatgtcat
     2821 tgctgaagac gggttaaagg caatcgaaga catgcgtact ggcgttgcat cggcagagtt
     2881 tatcaaagta tctgaaatca tcttcggtgt tgcaggtttg gtcggcggat tcggtgacaa
     2941 atatgcccgt aatactgccg ctcatgccat ccatgatgcg atttcggctt acttgcccgg
     3001 agtccatgac tatttgcatg gcgaaaaagt ggcttatggc attttttatc aattggcctt
     3061 ggaagggaaa tggcaagtga ttgatgagct gatcccgatg tacgagagct tgcatctacc
     3121 aaagtctttg acggaaatgg gcgagtaccc attatcggaa gaagcgttgg acaacatagt
     3181 ggcattgatc aacaaaaaag aaaaagtgca tttgctgccg atcccgatca atgaaacaat
     3241 cttgaaaaaa gcaataatag atttagaaaa atacataaat atggaggtat aggcaatgga
     3301 aaatattacc gcaatacaaa gtttgctgat tgcactttgg gtaggggcag tcatgtcacg
     3361 tgcattttta ggtggagcga cattaacgtt acgtttttcc ccgttgatga cgggtttgat
     3421 cgcaggtatc gtaatggggg atgttcaaca agcaatgatt atcacagcag cgatccaact
     3481 aatttatatg ggggttttct ctccaggggg aacgatgcct tctgaaccag caatcgcagc
     3541 agcgatagcg gttccggtcg ctttgatggg taacctgcag cctgaagccg cgatagcggt
     3601 agctgttccg gtaggtttac tgggtgctta tctgtaccaa ttcagatttt tcctcaacac
     3661 gtttctgggt aaatacaccg ataaagccgt agaagacttg aatgacggcg gaatcgtgag
     3721 atcgatcatc ctttatccga caatcgcttc tttccttttg tttgtgccgt tagtattcat
     3781 tgctttgtac ttcggagcac ctgttatcgc tgatgtcatc accgctcttg aaggtacggt
     3841 tgtgttccac atccttacag ttgtcggcgg cggtttagcg gctatcggta ttgcaacaac
     3901 gatttatgtc atcggccgta aagactatat ggttttcttc ctgttagctt acttgatgag
     3961 cgttgtgttg gcttccttgt caatcacaat ggtaacttat gccattatcg gcgctttgat
     4021 tgcagccatt ttcgtactgg ctaaaggaca gggtgcaaaa gcagctcctg cttccgcggg
     4081 cagtgcctct cttgatgatg acgatgatga cgatttctaa aaagcaggca aattgaaagg
     4141 aagatgaaaa aatgataaac gctaatctag atgctaaaga aaaaaacata ttagcaccag
     4201 aagaaataac cactaaagat gttacgaaaa cctatttacg ctggcacttt gcgaacgaaa
     4261 ttccccactc attcgatcgt tatctggcgc cttcgctgct ttatgcgatg atgccgattc
     4321 tgcgcaaact gtacaaagat gaggatcaat tgagagctgc ctacaaacgt caattgctgt
     4381 tcttcaatac gcaactttca tggggtggcg gcgtcatcac agggctgatg tcttcgatgg
     4441 aacaagaacg cgccaaagaa gttgtgaacg gcgaagaagt taccatgact gacgatctga
     4501 tgtacaatac gaaagctggt ttgatgggcg ctttggcagg gatcggtgat tcgatcgact
     4561 cagggaccgt gcaatacatt ttcatcgcca tcgctgttcc ttgggcacaa atgggtagcc
     4621 caatcggcgc cttattccca ttcgtcgctt ttgctcttta ccaagtattg ttaggtgtat
     4681 tctttgcacg aagcgcattc aagacaggta aaaatgcgac tggtgtgatg cacagtgctg
     4741 gaatccagac tgtcatcgaa atgctgtcta tcctgggtat gttcatgatg gggattttag
     4801 ccggaaatta cgttaaagtt tcgtcaagtt tagaatttgc aatctccgga cggccatttg
     4861 tccttcagga aatgctggat aagatcatgc ctggcatgtt gccgttggct gttgttctgg
     4921 gtgtatactt ctactacatc aaaaaaggtc tgaaagtaac acgtgcgtta gtagggttga
     4981 cgctgattct gattgttctg gcaggcatag gtattctata aaaggttcat taagaaaggg
     5041 aagtgtgaaa aatgggagca gt
//
LOCUS       DAOMUJ010000078         5043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4599_length_5043_cov_2.685044, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000078 DAOMUJ010000000
VERSION     DAOMUJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_5043_cov_2.685044"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLT73_03135"
     CDS             <1..382
                     /locus_tag="PLT73_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HRM19408.1"
                     /translation="MIHDLGQAVIDTKAEKVYICNIMTQLGETEGFTDAEHIKVLHDH
                     LKAKFIDTVLVNTEKVPDEYLNQQADEEYLLQVRHDFKGLREENCRVISADFLDMKNG
                     GVYHDGKKVVSELINLISNIKTIC"
     gene            425..1366
                     /gene="whiA"
                     /locus_tag="PLT73_03140"
     CDS             425..1366
                     /gene="whiA"
                     /locus_tag="PLT73_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRM19409.1"
                     /translation="MSFASEVKKELTTLEVHREHAKAGLASLIRMSGSVSIYNQAFVL
                     NVQTENAAIARRMYVLLKDHYSFESELLVRRKMKLKKNNVYIVRLKQGVRELLSDLNI
                     FDGLSFKTQVSEDIMNNNQKERSYLRGAFMAGGSVNSPETSRYHLEIYSNYEDHNQDI
                     CDMMNHFDLNARTIERRNGFITYLKEAEKIADFLALIGAHNAMMKFEDVRIIRDMRNS
                     VNRLVNCENANMNKTIDAAAKQVANIEFIEATVGLEKLPDKLKEIAVIRLENPDISLK
                     ELGEMIPSGAISKSGINHRLRKINDFADSLRMRKTIR"
     gene            1484..1819
                     /locus_tag="PLT73_03145"
     CDS             1484..1819
                     /locus_tag="PLT73_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM19410.1"
                     /translation="MNQVSLIGRLVRPIQVQQVNGERQVCNNTLAVQRLRKADEGQPQ
                     ADFIPVVFWGATANLVEQYCAKGNQIGVNGHLVSRSYVNKQEQHVYVIELVVEELYFV
                     EAKREQEAV"
     gene            1984..2697
                     /locus_tag="PLT73_03150"
     CDS             1984..2697
                     /locus_tag="PLT73_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM19411.1"
                     /translation="MEILMIEDNEAVCEMMAMFFENEGWQAVFKHDGKEGLETFAAAD
                     RKWDMIILDLNLPSMDGMQVCREVRKISQFVPIIMLTARDSESDQVIGLEIGADEYVT
                     KPFSPLTLIARIKAMHRRAKVDHAQAQATDDFDVLTDHVKISTITREAYLDGKQIESL
                     TPKEFELFALLAEHPKQVFSREHLLTRIWEDPYYGDERTIDAHIKKLRQKIEAVGPQV
                     IQTVWGVGYKFDDSGVEDK"
     gene            2694..4115
                     /locus_tag="PLT73_03155"
     CDS             2694..4115
                     /locus_tag="PLT73_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM19412.1"
                     /translation="MKYVYQQILAFFALILITVSTTGILIIQYVTSNIYDEKEAQLYG
                     YAESIIDQNMTIQQLENGSTLVSNQDVSFAIFDAEDRMVYPQATDLYTSGISAEDFER
                     LSEGDRISLTERDRGFLNEKKRFLTVYLPLFNATTNEFTGFIAVGSPVKGIEDEINEI
                     EHNLLVAVLTSGGIAIVLSLGIANYLTRRIKRMRRATHEIASGNFDISLENHHKDEFD
                     ELSEDFNQMARSLKASNEEVERQENLRRQFMMDVAHEMRTPLTTINGILEGIQHHMIP
                     EGSRDRSMQLMQKETKRLIRLVNENLDYEKIRSNQIILVKQEFSAKEALENVAEQMRT
                     KAQAKDDLIIVQVDAADRIYADYDRFIQIMVNLTQNAIQFTKNGTITLSSFPDGDQQI
                     IQVKDTGIGIEKKDITSIWERFYKVDVSRKNTKFGESGIGLAVVQSLVMNHKGTIDVE
                     SNPGEGTTFTITLPTKAAIEKNS"
     gene            complement(4159..>5043)
                     /locus_tag="PLT73_03160"
     CDS             complement(4159..>5043)
                     /locus_tag="PLT73_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM19413.1"
                     /translation="EDEMNNTFTLDTGDETIDGYFNTAHYADEALAEFFQYMKDAGLY
                     DNTLFVLYGDHFGISSSRNETLAPLIGANPDLWGAYDDAMMQRVPFIIHNPGSGTGQI
                     SDVYGGQIDILPTVMHLLGVDTSAYVQLGQDLMSAQNEGIVVFRNGSIVTSEYTILGN
                     TVYHTQTGTLAYQTEEVVEKVAQIRAQAELQLAISDQIINGDLLRFYTPDGFVPVDKS
                     LHGYVDSPSRLEEDIAELGDLNTSLYYTNNGVSTVPLYQTDAPEFPANQAIAEENAMQ
                     EQPAAEEVPAEGQTETVE"
BASE COUNT         1465 a         1104 c         1283 g         1191 t
ORIGIN      
        1 gatgatccat gacctcggcc aagcggtcat cgataccaaa gccgagaaag tctacatctg
       61 taacatcatg acgcaattgg gcgaaacaga gggcttcact gatgctgagc atatcaaggt
      121 attgcatgat catctgaaag cgaaattcat tgacaccgtc ctggtgaaca ctgaaaaagt
      181 tccggatgaa tacctgaatc agcaagctga cgaagaatat ttgctgcagg tccgccatga
      241 tttcaaagga ttgcgcgaag aaaactgccg ggtcatttct gccgatttcc tggatatgaa
      301 gaacggcggc gtataccacg acggcaaaaa agtcgtttcg gaactgatca atctcatttc
      361 caatatcaaa actatctgtt gacctttttt ctgatcgaaa gaagctgtcg gaaaggaggc
      421 gtatatgtct tttgcatctg aagtaaagaa agaattgacg acattggaag tgcaccgcga
      481 gcatgcgaaa gccgggttgg cctcattgat ccggatgagc ggttcggtca gcatctataa
      541 tcaggctttc gtcctgaatg tccagacgga aaatgcggca atcgcaagac ggatgtatgt
      601 actcttgaag gaccactatt cattcgaaag cgaattgctg gtccgtcgga aaatgaaact
      661 gaagaaaaac aacgtctata ttgtgcgcct gaaacaaggc gtccgggaac tgctgagtga
      721 tctgaatatt ttcgatggac tgtcgttcaa gacgcaagtt tccgaagata tcatgaacaa
      781 taaccagaag gagcgctcct acctacgcgg agcttttatg gccgggggat cggtgaacag
      841 cccggaaacg agccgttacc atctggaaat ctattccaat tatgaggacc ataatcagga
      901 catctgcgac atgatgaatc acttcgacct gaatgcgcgg acgatcgagc ggcgcaacgg
      961 tttcatcacg tatctgaagg aagccgagaa gattgctgat tttctggcat tgatcggtgc
     1021 ccataacgcg atgatgaaat tcgaggacgt ccggattatc cgcgatatgc gtaattctgt
     1081 caatcgtttg gtgaattgcg agaacgccaa tatgaacaaa acaatcgatg cggcagcaaa
     1141 gcaagtagcc aacatcgaat tcatcgaagc aacagtaggt ctggagaaat taccggataa
     1201 gctgaaggaa atcgcagtca tccgtttgga gaatcccgac atcagcctta aagagctggg
     1261 cgaaatgatc ccaagtggag ccatatcaaa gtccggcatc aatcaccgtt tacgaaaaat
     1321 caatgatttt gcagattcgt tgcggatgag aaaaaccatc cgctgataaa aaaataaact
     1381 cggagacaaa aaagtctttg ggtttatttt atttattaaa atgcacacct catttttggc
     1441 ggatattaca aacagtaagt caagctgaag gaggaatgga aggatgaatc aagtatcatt
     1501 gatcgggcgt ttggtgcggc ccatccaagt gcaacaggtg aatggggaaa ggcaagtttg
     1561 caacaacaca ctggcggtgc agcgtctcag gaaagcggat gagggtcaac cgcaagcgga
     1621 ctttatacct gtcgtgtttt ggggagcaac cgccaatctg gtcgaacagt attgtgctaa
     1681 gggcaatcag atcggtgtca acggccattt ggtttcgcgt tcctatgtca acaagcagga
     1741 gcagcacgtc tatgtcatcg aattggtcgt agaagaattg tattttgttg aagcgaaaag
     1801 agaacaggaa gcagtctgat gctcccgccc aaaagccccg gattctgggg ctttttttga
     1861 ttggaaccgg cgatttccca attatttcac agaaacttca aacgtatgta gtatcgtaga
     1921 ctggaaatga tgccgcttat gggaaaaaaa gatataatgg aggaaatgaa aaggggaatc
     1981 gctatggaaa ttttgatgat agaagacaat gaagccgtct gcgaaatgat ggcgatgttt
     2041 tttgagaatg agggctggca agctgtattc aagcatgatg gaaaagaagg tctggagacg
     2101 ttcgcggcag cagacagaaa atgggacatg attatcctcg atctgaacct gccgagcatg
     2161 gatgggatgc aggtctgtcg cgaagtaagg aaaatttccc agttcgtacc gatcatcatg
     2221 ctgacagcgc gcgattctga aagtgatcag gttatcggcc tggaaatcgg ggcggatgaa
     2281 tatgttacga agccgttcag tccgttgacg ttgatagccc gcatcaaagc gatgcaccgt
     2341 cgcgctaagg tcgatcacgc gcaagcgcag gccactgatg attttgacgt actcaccgat
     2401 catgtcaaaa tcagcacgat cacgcgcgaa gcctaccttg acgggaaaca aatcgaaagc
     2461 ttgacgccga aagaatttga actgttcgcg cttttggccg aacacccgaa acaagtattt
     2521 tcgagagagc accttttgac ccggatctgg gaagaccctt actacgggga tgagcgcaca
     2581 atcgatgccc acatcaagaa attacggcaa aaaatagaag cggtcggacc gcaagtgatc
     2641 cagacggttt ggggagtcgg ctacaaattc gatgatagtg gagtagagga taaatgaagt
     2701 atgtctatca acaaatcctg gccttttttg ctctgattct gataaccgtg agtacgacag
     2761 gcatcctgat catccaatac gtcaccagta atatctacga cgaaaaagag gcacagcttt
     2821 acggctatgc cgaatcgatc atcgatcaga acatgacgat ccaacaattg gaaaatggat
     2881 ctaccttggt ttccaaccag gatgtctcgt tcgccatctt cgatgccgaa gaccgcatgg
     2941 tttatccgca agcaacggat ctgtatacga gcggaatcag cgcggaggat tttgagaggc
     3001 tatcggaagg cgaccggatt tctttgactg aacgggatcg tggcttcctg aacgaaaaga
     3061 agcgcttctt gacggtctat cttcctctgt tcaatgcaac tacgaatgaa ttcacaggct
     3121 tcatagcagt cggttcgccg gtaaaaggca tcgaggacga aatcaatgag attgaacaca
     3181 acctgcttgt ggctgtgctg acttccgggg gcatcgcgat tgtattgagt ctcggcatcg
     3241 ccaactatct gacaaggcgc attaaacgca tgcgcagggc cacccatgag atcgcttcag
     3301 gcaatttcga tatctcactg gagaatcatc acaaagatga gtttgatgag ctgtcggagg
     3361 atttcaacca aatggcaagg tctttgaaag cctccaatga ggaagtcgaa cgccaagaga
     3421 atttgcggcg ccagtttatg atggatgttg cccatgaaat gcgtacgccg ctcacgacaa
     3481 tcaacggcat tctcgaggga attcagcatc atatgatccc cgaaggcagt cgggaccgga
     3541 gtatgcaact gatgcagaaa gaaaccaaac ggctgattcg tttggttaat gagaatcttg
     3601 attatgaaaa gatccgctcc aaccagatta ttttagtgaa acaggaattt tcggctaaag
     3661 aagcattgga gaatgttgcg gagcagatgc ggacaaaagc gcaggcgaag gacgatctga
     3721 tcatcgttca ggtggatgca gcggaccgga tttacgcgga ctatgatcgc tttatccaga
     3781 tcatggtgaa cctcacccag aacgctatcc agttcacgaa aaacggaacc atcaccctgt
     3841 cgtctttccc ggatggcgat cagcaaatca ttcaggtcaa agacacgggc atcggcatcg
     3901 agaagaagga tatcacgagc atctgggaaa ggttctacaa agtggatgtt tccagaaaga
     3961 acacgaaatt tggcgaatca ggaatcggtt tggcggtggt ccaatcatta gtgatgaacc
     4021 ataagggaac cattgatgtg gagagcaatc cgggcgaggg aacaacattc acgattacat
     4081 taccgacgaa agcagcaatc gaaaaaaata gttaaaaaaa tagctgccgg aatttttaaa
     4141 ttccggcagc tattttggtt attcaactgt ttctgtctgt ccttctgctg ggacttcctc
     4201 agcagcgggt tgttcttgca ttgcattttc ttcagcaatg gcttggttgg ccgggaactc
     4261 aggagcgtcc gtctgataaa gcggcacggt tgaaacgccg ttgttcgtat agtacaagga
     4321 agtgttcagg tcacccaatt ctgcgatgtc ctcttccaat cttgaagggg aatccacata
     4381 gccgtgcagg cttttatcaa ccggaacgaa tccatccggt gtgtagaagc gcaacaaatc
     4441 accgttgatg atctgatcgg agatggccaa ctgcagttct gcttgcgccc ggatctgagc
     4501 aactttttcg actacttctt ctgtctgata agccagcgtg cctgtttggg tatgataaac
     4561 ggtatttcct aagatggtat attcgctcgt cacgatgctg ccattacgga aaacaacgat
     4621 cccctcattt tgcgctgaca tcagatcctg tccaagttgg acataggctg atgtgtcaac
     4681 acccaaaaga tgcatcacgg taggcaagat atcgatctgg ccaccataaa catcactgat
     4741 ctggccggta ccggaaccag gattatggat gatgaaagga acacgctgca tcatggcatc
     4801 gtcataggca ccccacaaat ccggattggc tccgatgagc ggcgccaacg tttcgttgcg
     4861 ggaactggaa ataccgaagt ggtcgccgta caatacgaaa agcgtattat cataaaggcc
     4921 tgcgtccttc atatattgga aaaattcagc caaggcttca tcggcataat gagcagtatt
     4981 gaaataaccg tcaatcgttt cgtcaccggt atccagagtg aaggtgttat tcatctcgtc
     5041 ttc
//
LOCUS       DAOMUJ010000079         5038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4608_length_5038_cov_2.795505, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000079 DAOMUJ010000000
VERSION     DAOMUJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_5038_cov_2.795505"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /gene="tuf"
                     /locus_tag="PLT73_03165"
     CDS             <1..234
                     /gene="tuf"
                     /locus_tag="PLT73_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761693.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRM19414.1"
                     /translation="GRHTPFFTNYRPQFYFRTTDVTGVCNLPEGVEMVMPGDNVTMEV
                     ELIHPIAIEEGTKFSIREGGRTVGAGVVASISK"
     gene            complement(399..824)
                     /locus_tag="PLT73_03170"
     CDS             complement(399..824)
                     /locus_tag="PLT73_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM19415.1"
                     /translation="MNQSVVAEYQASGTTTASSGANNKITKIEIITNESKFEGLKKAL
                     NNIGITGMTVFDVFGYGMQKGHSTYYRGVETEADLLPKIRVEVVVSTVPVHEVVAAAR
                     KALYTGNIGDGKIFIYNVENVIRVSTGDEGKKALEYPTE"
     gene            complement(821..2185)
                     /locus_tag="PLT73_03175"
     CDS             complement(821..2185)
                     /locus_tag="PLT73_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM19416.1"
                     /translation="MENINYADTTFVFLATILVLLMTPALSLFYGGMVRKKNILSTTM
                     HSYAAIAVVSIQWILFGYSFVFGGYGPLIGDGSFALLNNVSFAPIDYAPTIPHQLFLM
                     FQLAFAVITPALISGSIAERMKFPAFLAFILLWTTFVYDPIAHWIWGNGGWIRELDAL
                     DFAGGSVIHISSGVSALVAAIYIGKRSNHDSIKPHNIPMTMIGAGLLLFGWYGFNAGS
                     ALAINDIALNAFIATNTSAAAAGLSWMFCEWLIHKKPTALGFVSGIVAGLVSITPGAG
                     FVSPFASLMIGLIGGIVCFYGVTVLKKKFAYDDALDAFGCHGIGGIWGGIATGIFATS
                     SINPAIEGGLLDGNIRLLVAEVISILAVVTYAGIVSYLLLKLISQFMQLRVSAQEEEI
                     GLDYAIHGETAYGSIAVALSEPNHSNSDGSVVLGELRMAFQKFQERSFTGNGPELAFA
                     NQKT"
     assembly_gap    2425..2434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2918..3226
                     /gene="rpsJ"
                     /locus_tag="PLT73_03180"
     CDS             2918..3226
                     /gene="rpsJ"
                     /locus_tag="PLT73_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRM19417.1"
                     /translation="MAKQKIRIRLKAYEHRALDQSADKIVETAKRTGATVSGPIPLPT
                     ERSLYTVIRATHKYKDSREQFEMLTHKRLIDIINPTTKTVDALMKLDLPSGVDIEIKL
                     "
     gene            3265..3903
                     /gene="rplC"
                     /locus_tag="PLT73_03185"
     CDS             3265..3903
                     /gene="rplC"
                     /locus_tag="PLT73_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRM19418.1"
                     /translation="MTKGILGKKVGMTQFFTANGELVPVTVIEATPNVVLQVKTNETD
                     GYEAVQLGYQEMREVLSNKPAKGHAAKAKTAPKRFIREFNNVELGEYEVGKEIKVDIF
                     AAGDIVDVTGTTKGHGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGAASFPSRVFKGKK
                     LAGRMGNDRVTIQNLEVVMVDVEKNVILIKGNVPGSKKSLIEIKVARKAANK"
     gene            3930..4553
                     /gene="rplD"
                     /locus_tag="PLT73_03190"
     CDS             3930..4553
                     /gene="rplD"
                     /locus_tag="PLT73_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRM19419.1"
                     /translation="MPNVALFKQDGTQNGEVTLNDEIFGIEPNENVVYDAIIMQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGSIRSPQWVGGGVVFGPTPRSYSYKLN
                     KKVRRLAIKSVLSTKVAEGDLIVVETLNFDAPKTKEFANVLKNLNVDTKVLVVVENDN
                     DFAALSARNLPGVTVVNETGINVLDVVSNNKLVLTQAALSKVEEALI"
     gene            4553..4837
                     /gene="rplW"
                     /locus_tag="PLT73_03195"
     CDS             4553..4837
                     /gene="rplW"
                     /locus_tag="PLT73_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRM19420.1"
                     /translation="MEATDVIKRPIITEASMLAMDEKKYTFEVDVRANKTLVEQSIES
                     LFGVDVKNVNIMNVRGKLKRMGKYKGYTKKRRKAIITLTENSKTIELFEN"
     gene            4872..>5038
                     /gene="rplB"
                     /locus_tag="PLT73_03200"
     CDS             4872..>5038
                     /gene="rplB"
                     /locus_tag="PLT73_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356204.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRM19421.1"
                     /translation="MAIKKYKPTTNGRRNMTGSDFAEITKTTPEKSLIEPIKRKAGRN
                     NQGKITVRHQAG"
BASE COUNT         1588 a         1047 c         1105 g         1288 t
ORIGIN      
        1 ggacgtcaca ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
       61 gttacaggtg tatgtaactt accagaaggc gttgaaatgg taatgcctgg cgataacgtt
      121 actatggaag ttgaactgat ccacccaatc gctatcgaag aaggaactaa gttctctatt
      181 cgtgaaggcg gccgtactgt aggcgctggc gttgtagctt caatctctaa ataattttat
      241 ttcaaaataa aatttaatgt gattaaaaaa atcccgagag cttctgctct tgggattttt
      301 tttgattcag acaaatggat attgacgtga accgggactg atacaagaca aaagaagatc
      361 atcggatgcg actccggatg atcttctttt atttggactt attcggttgg atattccaaa
      421 gctttcttgc cctcgtcgcc tgtgctgact cggatgacat tttctacatt atagatgaat
      481 attttcccat ctccgatgtt gcctgtgtac aatgcttttc tggcagcagc tactacctcg
      541 tggactggca cggtgctgac gacaacttcg acacgaattt tcggcagcag atcggcttcg
      601 gtctcgacgc ctcggtaata agttgaatgg cctttttgca taccatagcc gaatacgtcg
      661 aaaactgtca tgccagtgat gccgatgtta ttgagggctt ttttcagacc ttcgaatttg
      721 ctttcgttcg tgatgatttc aattttggtg attttattat tcgcgccact gctggctgtt
      781 gtagttccag atgcttggta ctccgcaact accgattgat tcaagtcttc tggtttgcga
      841 aagccagttc ggggccgtta cccgtgaaag agcgctcctg aaatttttgg aaggccattc
      901 tcaattcgcc cagtacgaca gatccatcgg aattcgaatg gttcggttca cttaatgcta
      961 cagcgatgct gccgtaagca gtttctccat gtatggcata gtccaatccg atttcttcct
     1021 cttgtgcaga gacgcgtagt tgcatgaatt ggctgatcag ttttaataag agataagata
     1081 caataccggc atatgtaact accgcaagga tgctgattac ttctgcaacc aaaagtctaa
     1141 tatttccgtc caataagccg ccttcgatag cagggttgat tgaacttgtt gcgaagattc
     1201 ctgtcgcaat cccgccccag attccgccga taccgtggca accgaatgca tcaagcgcat
     1261 catcataagc aaattttttc ttcagaacgg tgacaccgta gaaacaaacg ataccgccga
     1321 tcaatccgat cataagggaa gcgaacggac tgacgaaacc tgcaccagga gtgatggaaa
     1381 cgagccctgc tacaattccg ctgacaaatc cgagcgctgt cggcttcttg tggatcaacc
     1441 attcgcagaa catccaactt agacctgcag ctgctgcgga agtgttcgtt gcgatgaagg
     1501 cattcaaggc gatgtcgttg attgccagtg cgcttccagc gttgaaaccg taccaaccga
     1561 atagaagaag accggcacca atcattgtca tcggaatatt atgtggcttg atcgaatcat
     1621 ggttgcttcg tttgccgatg tagatggctg ccaccaaagc cgatacgcct gaactaatgt
     1681 gtatgacact gccgcctgca aaatcaagtg catccaattc gcggatccaa ccgccatttc
     1741 cccagatcca atgagcgata ggatcataga cgaatgtcgt ccataacagg atgaacgcta
     1801 agaaggctgg gaacttcatg cgttccgcga tacttccaga tatcaaagcc ggcgtgatga
     1861 cagcaaatgc caattggaac atcaagaata gttggtgggg aatcgtgggt gcatagtcga
     1921 tcggtgcaaa actaacgttg ttgagtaggg cgaaagaacc atcaccgatc aacggtccgt
     1981 aaccaccgaa aacaaatgaa taaccgaaga gaatccattg gatagatact acggcaatgg
     2041 ctgcatagct atgcatcgtt gtgctgagga tatttttttt ccgtaccatc cctccataaa
     2101 aaagggaaag cgctggtgtc attaataaaa ccaagattgt tgcaaggaat acgaaagttg
     2161 tgtcagcgta atttatattc tccattgata atctccttcc gctggcatct gacgagtgac
     2221 cagctgcttg tgatgttgtt aactgtacac gctgaacaaa agaaaatcat ctaaatgatg
     2281 acgggcattg tgatattaat tgacacggga aggtaccgaa atgctttatg aaaaagaatg
     2341 tatctctgtt tcgcaattgg tgtgcggact ctcatcggat aagggctgtg gctgcatgaa
     2401 aaaagtaaaa aagatgtaag tggcnnnnnn nnnnaaactg acatgaactg gctgctgcaa
     2461 caaaatatta ttcttgcatg agcctcccga aaaaattgtg tttcttctat ataaatataa
     2521 agggctctga taatccgaga aaagcagtcc gcgataaaaa aaggcgcggt aatagttgcg
     2581 gggcagaaat tcaaattgtt tgctatcccg atcggtttct gtataatagt gaaagggcaa
     2641 aaaagtattc tgagagcaag agcagaagat tttgaaaaaa tcggcactaa ttttattatt
     2701 attttcagaa aagacttgct ctgcttaatg cgattatgta taatatattg gtacggcatt
     2761 catggttatg aataatgtat tggttaccgt actatcggga accgcagcta agagacgaga
     2821 ggttgcgaca cacccggtcg ctttgtcacg gcgggtgtgc cggggaattt tcgtggagct
     2881 agtcttatga attacatctg acgaaggagg gaacaacatg gcaaaacaaa aaatacgtat
     2941 tcgtttaaaa gcatatgaac accgtgctct tgatcaatca gcggataaaa tcgtggaaac
     3001 agcaaaaaga acgggagcta ccgtttctgg tccgattcca ttgccgactg aaagatcact
     3061 atacacagtg atccgtgcta ctcacaagta caaagattct cgtgagcagt tcgaaatgct
     3121 tacacacaaa cgtctgatcg acatcatcaa cccaacaaca aaaactgttg acgctttgat
     3181 gaagcttgat ttaccaagcg gcgtagacat cgaaatcaaa ttataatcaa acctatcata
     3241 taaaaaacaa acggaggtgt taccatgacc aaaggaatct taggcaaaaa agtaggaatg
     3301 actcaattct ttactgcaaa cggtgagtta gtaccagtta cagttatcga agctactcca
     3361 aacgttgttt tacaagttaa aactaacgaa acagacggct acgaagcggt acaactaggc
     3421 taccaagaaa tgcgtgaagt attgtcaaac aaacctgcta agggtcatgc agcaaaagca
     3481 aaaactgctc ctaagcgctt cattcgtgaa tttaacaatg ttgagctagg agaatacgaa
     3541 gtaggaaaag aaatcaaagt tgatatcttt gcagccggcg acattgttga tgttactggt
     3601 accactaaag gacatggctt ccaaggcgta atcaaacgtc acggacaaag ccgcggacca
     3661 atggcccacg gatctcgtta ccatcgtcgt ccgggttcaa tgggtgctgc ttcattccca
     3721 tcccgcgtat tcaaaggcaa aaaattagca ggacgtatgg gtaacgatcg cgttactatt
     3781 caaaaccttg aagtcgttat ggttgacgtt gagaaaaacg taatcctgat caaagggaac
     3841 gtacctggtt ctaaaaaatc attaatcgaa atcaaagtag cccgcaaagc tgcaaacaaa
     3901 taatataaaa gagaggagga acgagaaaaa tgccaaacgt agccttattt aaacaagatg
     3961 gaacacaaaa cggtgaagta actttgaatg atgaaatctt tgggatcgag cctaacgaaa
     4021 acgttgtata cgatgcaatc atcatgcaac gcgcttcatt aagacaagga actcacgcag
     4081 tgaaaaaccg cagcgctgtc agcggtggtg gtcgtaaacc atggaaacaa aaaggaacag
     4141 gccgtgcacg tcaaggttca atccgttcac cacaatgggt tggcggcgga gttgtctttg
     4201 gaccaacacc tcgttcttac agctataaat taaacaagaa agttcgtcgc ttagctatca
     4261 aatcagttct ttcaacgaaa gttgctgaag gagacttgat cgtagttgaa acattgaact
     4321 tcgatgcacc aaaaacaaaa gaatttgcaa atgtgttgaa aaaccttaac gttgatacga
     4381 aagtattggt tgttgtagaa aacgacaacg attttgcagc attatcagca cgtaaccttc
     4441 ctggtgtaac agtcgttaac gaaacaggaa tcaacgtatt ggatgttgtt tcaaacaaca
     4501 aattagtctt gacacaagct gctctttcta aggtagagga ggctcttata taatggaagc
     4561 aacagacgtt atcaaacgcc caatcatcac ggaagcttct atgcttgcaa tggatgaaaa
     4621 gaaatatacc tttgaagtag atgtacgtgc taacaaaaca ttggttgaac aatctatcga
     4681 atcattgttc ggtgtagatg ttaaaaacgt aaacatcatg aatgttcgcg gcaaacttaa
     4741 acgtatgggt aaatacaaag gctatacaaa aaaacgccgt aaagcaatca tcactttgac
     4801 tgaaaattct aaaacaatcg aattattcga aaattaatct tttgaaataa tcaaatagga
     4861 ggggaaaaaa cgtggcgatt aagaaataca aacctaccac aaacggccgt cgtaatatga
     4921 ctggttctga tttcgcagag atcacaaaaa caacacctga gaaatctttg atcgaaccaa
     4981 tcaaaagaaa agccggtcgt aacaaccaag gtaaaattac cgttcgtcat caagcggg
//
LOCUS       DAOMUJ010000080         4967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4715_length_4967_cov_2.475977, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000080 DAOMUJ010000000
VERSION     DAOMUJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4715_length_4967_cov_2.475977"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1377)
                     /locus_tag="PLT73_03205"
     CDS             complement(160..1377)
                     /locus_tag="PLT73_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19422.1"
                     /translation="MPEKHNKILSSKNWAPHTHQALNAVISSYGNQSSSFDPAAPPYV
                     VFDFDNTSAIMDIEDTLMLYMLLHLDYRLTPDQFHAILTDGLENVGATVDTLLDKTNP
                     LATIGNIADDIKVAYAWLYKQYEGFMQGGTLSLEEAKKSSYYEEFAAKIRLFYTVING
                     DFKRKAGYPWMTYLFAQRSSEELRQLAYQSISYWLQYGKFERVTLTSPADLTSKAGVI
                     VSHYDTGLAFPNELKELYHVLQANGIVVYVISASPVDVVQVAATHPDFGYGLDNEHVI
                     GMYYNKDENGLIQPSMQPGKNITKGPGKTAVITEQLMPKHNEKQPLMLFGDSTGDYDM
                     MMELKDTKLCVLFNRYMNDDTQKLAREAFRTIEDENPRIVLQGRDENKGSLRPSEKTI
                     LLGTQEEVLIHEA"
     gene            1846..2568
                     /locus_tag="PLT73_03210"
     CDS             1846..2568
                     /locus_tag="PLT73_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM19423.1"
                     /translation="MIKLEHVNKYFGANHVLKDINLEVAEGEKVVIIGPSGSGKSTTV
                     RCMNFLEEPTDGKVFIDGTEVTPKNKLDIVRHSSAMVFQQFNLYPHMTVLGNLTIGPM
                     KLQGKTKKEAEEMAYEYLDIVGLREKANVYPSTLSGGQQQRVAIARALCSQRKIILFD
                     EPTSALDPETVQEVLDVMIKLSSMNITMVVVTHEMGFARQVADRVIFMADGMIVEEGT
                     PEHFFDNPTNERTKAFLGKILR"
     gene            2609..3442
                     /locus_tag="PLT73_03215"
     CDS             2609..3442
                     /locus_tag="PLT73_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM19424.1"
                     /translation="MKKKNLWVSLCLSAALFLGACGSDATDTADSAAGSATDASGQVT
                     AETIKEAGVLKVGVKEDVPNFGLRNTETNEIEGFEIDITKKIAEEILGDAEAIELVPV
                     TAKTRGPLLDNGEVDMVIATFTVTEERKETYNFSEAYYVDAVGLLVKKDKGYTGLADM
                     DGATIGVAQSSTTADAINAEAEQYGISLSYSEYATYPEIKAALDSGRVDAFSVDRSIL
                     AGYLDDSTQILKDRFATQDYGVAIKKSNTELATTVNDLITAWGEDGTLDAMITEWGLG
                     E"
     gene            3442..4164
                     /locus_tag="PLT73_03220"
     CDS             3442..4164
                     /locus_tag="PLT73_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM19425.1"
                     /translation="MRSTNPFALWRWESLLENSSDIGAGFIRTLQVAVLALLLALVIG
                     VVVGTMSTAKGRLPRAVARIYVEMFQNIPLVIQIFFMYNGLAMAGLVLSEFTIGVVGV
                     GMYHGAYIAEVVRAGILAVPKGQEEAAYSEGFNYIQTMRYVILPQMVKIILPPLTNQA
                     VNLIKNTSVLAIIAGADLMYVADSYASYSLNYGPAYAVAGLLYFLLCFPLATFARKYE
                     QKLKDNDTVVVNVGVDIPEVME"
     gene            4161..4820
                     /locus_tag="PLT73_03225"
     CDS             4161..4820
                     /locus_tag="PLT73_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM19426.1"
                     /translation="MMESIRTVFTPSNVSFMMEGLKLALLISVGVVILSILFGTVLGL
                     LRNYEKKVIGKLAGAYIELFRNTPLLLWMLSCSFLIPGSTITLKGSFALFLYTSAVVA
                     EIVRGGLNSIPKGQFEAAHSQGFSFMQSLWYIVLPQAFKKIIPSLLSQVITTIKDTSF
                     LAGLGIMEFTRSGQVILGKVTKTQEVFMIYAFLALVYFIICFTLSAVVRSWHKRNAQN
                     A"
BASE COUNT         1373 a         1020 c         1204 g         1370 t
ORIGIN      
        1 atttttatta tggtttgcct ttggggcaac tgtgggatgt ccgataacca cgaagaatcg
       61 gacaagtagc aagcaagcat atcggcctac acgaaaaagc acaaaatact caaaaaagaa
      121 gccccacaca ataaaaaatt gtgcgtaggc ttcttttgtt tacgcttcgt ggatgagtac
      181 ttcttcttgg gttcccaaaa ggatcgtctt ctcggaaggg cgcaggctgc ctttattttc
      241 gtcacgaccc tgcagaacga tgcgcggatt ttcgtcctca attgtgcgga aagcttcgcg
      301 cgccaatttt tgagtgtcat cattcatgta acggttgaag agaacgcaga gtttggtgtc
      361 cttcagttcc atcatcatat cgtagtcgcc ggtgctgtct ccgaagagca tcagcggttg
      421 tttttcgttg tgcttcggca tcagctgttc tgtgatgacg gctgttttac cgggaccttt
      481 cgtaatattt ttgcctggtt gcatgcttgg ctggatcagg ccgttttcat ccttgttgta
      541 atacatgccg atgacgtgtt cgttgtccaa tccgtaaccg aagtccggat gcgttgcggc
      601 gacttggacg acatctacag gtgaagccga aatgacatag acgacgatgc cgtttgcctg
      661 cagaacatga tagagttcct tcaactcatt cgggaaggct aaaccagtat cgtaatgcga
      721 tacgatgacg ccggctttgc tcgtcaaatc agccgggctc gtcaaggtca cgcgttcgaa
      781 ttttccgtac tgcaaccaat aagaaatcga ttggtaagcc aactggcgta actcttcgct
      841 gctgcgttgc gcaaaaaggt aagtcatcca aggatagccg gcttttcttt tgaagtcacc
      901 gttgatgacc gtgtagaaca aacgaatttt tgcggcgaat tcttcataat aagaagactt
      961 ttttgcttct tccaacgaga gggtcccgcc ttgcataaaa ccttcatatt gcttgtacaa
     1021 ccaagcatag gctactttga tatcgtcagc gatattgccg atcgtagcaa gcggattggt
     1081 cttatcgagg agcgtgtcca ctgttgcgcc gacattctcg aggccatccg tcaaaatcgc
     1141 atggaactga tcaggcgtca aacggtaatc caaatgcagc aacatataaa gcatcaaagt
     1201 atcttctata tccatgatgg cggaagtgtt gtcgaaatca aagacaacat aaggaggggc
     1261 agccggatcg aatgaactgc tttggttccc gtaagaagag atgacagcat tcagcgcttg
     1321 gtgtgtatgt ggtgcccagt ttttggaaga aagaatttta ttatgttttt ctggcaaagt
     1381 aagtacccct ttatcatgga attttcatac gcagtcattt tatcaaaaaa cggctgaaag
     1441 atatactgaa atgtgctcct ttagttcaat caaacaagaa cattgaaatg aaatgagaat
     1501 tatcgataag tttctatgtc ggttatttat tttaaatgaa gctttttgcc tcaggatgct
     1561 acattaaagc agaaagattt atataaaagc gagaatcgtg gaaggctttc tcataaaaca
     1621 ggcataaaat aggcggtttt taacaattaa gtaagaatac gagaaattcg tgttagaaaa
     1681 tctaacattc tcgcggaaga atttaattga tttttaatca tgctttcatt ataattttgc
     1741 ttaacggtat aaatataaga agaggtcctt tcttgacaag gatccaatcc atgctcttga
     1801 aattaaagca gcgtgatggc gagcataaaa ggggttgagg caaaattgat taagttagag
     1861 cacgtaaaca agtatttcgg cgccaaccat gtgttgaagg atatcaacct tgaagttgcc
     1921 gaaggggaaa aagtggttat tatcgggcct tccggttcag gtaaaagtac gacagtccgt
     1981 tgtatgaatt tcctcgaaga gcctacagac ggaaaagttt ttatagacgg cacagaagtc
     2041 acacctaaga acaagttgga cattgtccgt cattcctctg caatggtttt tcaacaattt
     2101 aatttgtatc cgcatatgac cgtgttaggc aacctgacaa tcggccctat gaagctgcaa
     2161 gggaaaacca aaaaagaagc ggaagagatg gcttacgaat atttggatat cgtcggattg
     2221 cgagaaaagg cgaacgtcta tccttcaaca ctctcaggcg gacaacaaca aagagtagca
     2281 atcgcaagag cactttgttc acagcgaaag atcattcttt ttgatgaacc cacttcggca
     2341 ttggaccctg aaacggttca ggaagtattg gatgtcatga tcaaactttc cagcatgaac
     2401 atcacgatgg ttgtggtgac gcacgaaatg ggctttgcga gacaagttgc cgatcgggta
     2461 atcttcatgg ctgacggcat gattgttgaa gaaggcactc cggagcactt tttcgacaat
     2521 ccgacaaacg agcggacgaa agccttcttg ggtaaaattt taagataacc gatcttttcg
     2581 gaatacaaca ttataggagg aattgggtat gaaaaagaaa aatctatggg tttcgttatg
     2641 tttatcggct gcattgttct tgggtgcttg cggtagtgat gctacggata cagcagattc
     2701 tgcggctggt tccgcaacag atgcttccgg tcaagtgacc gcagaaacaa tcaaagaagc
     2761 aggcgtattg aaagtcggcg ttaaagaaga tgtgccgaat tttggattaa gaaacactga
     2821 aacaaacgaa atcgaaggtt ttgaaatcga catcacaaaa aaaattgctg aagaaattct
     2881 gggcgatgct gaagcgattg aattggttcc ggtaactgca aaaacgcgtg gtccattatt
     2941 ggataacggc gaagtggata tggtcatcgc aacattcaca gttacagaag aacgtaaaga
     3001 aacctataac ttctctgaag cttattatgt ggatgcagtc ggcctgttgg ttaagaaaga
     3061 caaaggctac accggcttgg ctgacatgga cggtgctaca atcggggttg cccaaagttc
     3121 aacaactgct gatgcaatca acgctgaagc tgagcaatac ggcatttcct tgagctactc
     3181 agaatatgct acttatccag aaatcaaagc tgctttggat tccggccgtg tggatgcatt
     3241 cagcgtggac agatccatct tggctggcta cttggatgat tcaactcaaa tcctgaaaga
     3301 ccgcttcgct acacaagatt acggtgttgc gatcaagaaa tcaaacacag aattggcaac
     3361 gactgttaat gacttaatca ctgcatgggg agaagacggc acccttgatg caatgattac
     3421 tgagtgggga ttgggcgagt aatgagaagt accaatccat tcgctctctg gcgttgggaa
     3481 agccttcttg aaaacagcag cgacattgga gccggattta tccggacact ccaagtcgct
     3541 gtgttggcgt tgttgctggc tttggtcatc ggggttgtcg tgggaaccat gtccacggca
     3601 aaaggccgat tgcctcgtgc tgttgccaga atatacgtgg aaatgtttca gaatatcccg
     3661 ttggttatcc aaatcttctt catgtacaac gggcttgcga tggcaggctt ggtgctgagc
     3721 gaatttacga tcggggtagt cggtgtaggg atgtatcacg gtgcctacat cgctgaagtc
     3781 gttcgtgcag gtatcctcgc tgttccgaaa ggccaagaag aggcagctta ctcagagggc
     3841 ttcaactata tccaaacaat gcgttacgtc atcttgccgc agatggtcaa aatcattttg
     3901 ccgcctttga ccaatcaagc ggtcaacttg atcaagaata cttccgtatt ggcgatcatc
     3961 gccggagcgg acctcatgta tgtagctgat tcttatgctt cctacagttt gaactacgga
     4021 cctgcttatg cggttgccgg attgctttac ttcctgctat gcttcccgct tgcaacattt
     4081 gcgcggaaat acgagcaaaa actgaaagac aatgatacgg ttgttgtcaa cgttggcgta
     4141 gatattccgg aggtgatgga atgatggaat cgatcagaac agtcttcaca ccatcgaatg
     4201 tgtcgttcat gatggaagga ttgaaactgg cattactgat ttctgtcggt gtcgtcatcc
     4261 tttcgatact cttcggtacg gtattgggtc tgttgcgtaa ttatgagaaa aaagtaatcg
     4321 gaaaactggc tggtgcctac atcgagcttt tccggaatac accgttactt ttgtggatgc
     4381 tttcgtgttc tttcttgata cctggtagca caataacctt gaaaggatct tttgccttgt
     4441 tcctgtacac atcggccgtt gtagccgaaa tcgtgcgcgg aggattgaat tcgataccga
     4501 aagggcaatt cgaagctgcc cattctcaag gtttttcctt catgcagtct ttgtggtaca
     4561 tcgttttgcc gcaagcattc aagaagatta tcccttcact gctgtcgcaa gtgatcacta
     4621 ccatcaagga tacttctttc ttggctggtc tggggatcat ggaattcaca agaagtgggc
     4681 aagttattct cggaaaagtg acgaaaacac aggaagtgtt catgatttat gccttcctgg
     4741 cgttggtgta tttcatcatc tgctttacgc tttcggcagt cgtccgaagc tggcacaagc
     4801 gcaatgcgca gaatgcttaa aatttgaaaa gctcccgaat tacagcgatg tattatgctg
     4861 taattcggga gctttttgtt tgattgaaaa agttctgtac aggtggtttc ctttgggctg
     4921 tcggataaac cagaagaatc cgacaaccgg agagcaaacg ctctgtg
//
LOCUS       DAOMUJ010000081         4966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4717_length_4966_cov_2.137854, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000081 DAOMUJ010000000
VERSION     DAOMUJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_4966_cov_2.137854"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLT73_03230"
     CDS             complement(<1..1016)
                     /locus_tag="PLT73_03230"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM19427.1"
                     /translation="MKVIKFGGSSLANGTQLKKVLQIVKDDADRRIVVVSAPGKRNDS
                     DEKVTDLLIKLADEVKNKEDHKKTLKTILKRYQDIVEELEIDPSVMEIISQNYEELIQ
                     AKFYNELYALDAYKASGENNSAILVAAFFNKEGIPAKYINPKDAGLLVTDNPGNARVL
                     PESYKNLYALRDSKEVIIFPGFFGYTKEGQLLTFSRGGSDISGAIVANGVKASLYENF
                     TDVDAIYVANPKMVKHPKKIKQLTYREMRELSYSGFSVFHDEALQPAFSAGIPVVVKN
                     TNNPSAPGTSITRTKPENKQIVSGIASSTGFMSIYIGKYLMNREVGFGRRVLQIFEDF
                     NLNF"
     gene            1229..1591
                     /locus_tag="PLT73_03235"
     CDS             1229..1591
                     /locus_tag="PLT73_03235"
                     /inference="COORDINATES: protein motif:HMM:NF027436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HRM19428.1"
                     /translation="MKKNNPWRIVVGLSVIASISSALFKSNRNAAKSPSTTYTPYIGT
                     WKNDAQSLKVEITDNLEILLNNKKLKATLEEAKPNELVFRDHFGYYLILKKDTSPTLT
                     IYDEAEEQEFYFKREIDK"
     gene            complement(1715..4111)
                     /locus_tag="PLT73_03240"
     CDS             complement(1715..4111)
                     /locus_tag="PLT73_03240"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRM19429.1"
                     /translation="MAFDVQEFKKEFQKKFEELFAYGYESGSKTEQYIALGDLVRGLY
                     SDDWIQTVTDYNKKKEKQVFYFSMEFLPGRMLKSNLLNMGIMDCVKQGLEELGLDLDE
                     VARAEVDPALGNGGLGRLASCFMDSIASTGLAGNGNGIRYRYGLFKQKFINGYQIELP
                     ENWLRNRNVWEVRKENKAVIVRFGGDVSFTQDGDGKLRVHHENTRDILAVPYDTAQIG
                     YNNGIINNLRLWSAEIPYGDEARFSTEEQREEVKRITEVLYPDDSNYEGRLLRVQQEY
                     FFSSAGVQSIVRYFKQLKLDWSLFPDKIAIHINDTHPTLAIPELMRILLDEEELTWDQ
                     AWEITKKTVSYTNHTILQEAMERWPETMIADLLPRIYQIIQEINRRHIDKKMPLYGEE
                     LTQRTAIIANGQIKMANLAIIGSHSVNGVAKLHSDILMADTLHDFYVMYPQKFNNKTN
                     GITQRRWVQIANENLTEMLDETIGTEWKSDPEEIKVLKAHRDNKIVLDKLEQVKLANK
                     KRFADYVLKEMHIEIDPNALFDVQIKRLHAYKRQLLNVLHILDRYLQLKDDPKANLQK
                     RVFIFGAKAAPSYYYAKQIIKLINAIADLVNNDPAINDKIKIVFVENYGVSLAELIIP
                     AADISEQISLAGKEASGTSNMKLMSNGAVTMATLDGANVEIRDNVGDENIFIFGLKSD
                     EVQEYYRNGVYNSREIYEKNPRLKRILNLLIDGSIPGVETEGRDIFDSLVMYNDEYFL
                     LKDFDGYLQAQYEADVAFSDRDRWNRMALMNIASSGPFSSDYTILRYADEIWKIKPQS
                     "
     gene            complement(4130..>4966)
                     /locus_tag="PLT73_03245"
     CDS             complement(4130..>4966)
                     /locus_tag="PLT73_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM19430.1"
                     /translation="KGGINFSDVVTTVSPSYANEIQTQEFGEGLEGTLGYNSWKIRGI
                     INGIDYDVNDPETDPKLEYHFTADDLTGKAKNKAALQERLGLEVNPDIPLITSVGRLT
                     DQKGYQLVQEKTEELLNARDVQIAILGTGEEQYENSFRYFAAKYPDRFAAVIDFDISL
                     AQQMYAGADLFLMPSAFEPCGLSQMISLRYGTLPIVHETGGLKDTVIPYNSYTGEGTG
                     FTFIDFSGYALLGTIYRALDVYENQPKAWAKMVREAMSQDFSWKEPAKDYLDVYQSLV
                     NE"
BASE COUNT         1206 a         1217 c         1068 g         1475 t
ORIGIN      
        1 tcgaagttca agttgaaatc ttcgaatatc tgcagcactc tgcgtccgaa tccgacttcg
       61 cggttcatca ggtatttgcc gatgtagatg ctcatgaatc cagtcgagct ggcaataccg
      121 gaaacaatct gcttgttttc aggcttcgtt ctcgtgatgc ttgtgcctgg agctgatgga
      181 ttattggtat ttttgacaac aaccgggatt ccggcgctga aggccggttg taaagcttcg
      241 tcatggaata cggaaaatcc gctgtaggat aattcacgca tttcacgata agtcagttgt
      301 ttgatttttt tcggatgttt gaccattttc gggtttgcaa cataaatggc atcaacgtcc
      361 gtgaagtttt catacaggct ggctttgacc ccgttcgcga cgattgcgcc agagatgtcc
      421 gatccgcctc tcgagaaagt caacagttgg ccttctttcg tgtaaccgaa gaatccaggg
      481 aaaatgatga cttcttttga atcccgtaat gcgtaaaggt tcttgtatga ctcaggtaat
      541 acccgtgcgt ttcctgggtt gtcggtgacc aaaagtccgg catccttcgg gttgatgtat
      601 ttggccggaa tgccttcttt gttgaaaaat gcagccacaa gaatggcact gttgttttca
      661 ccgcttgctt tgtaagcatc caacgcatac agctcgttgt aaaacttcgc ctggatcaat
      721 tcttcatagt tttgggaaat gatttccatg acactcggat cgatctccaa ttcttcgacg
      781 atatcttgat atcttttcag gatcgtcttt agtgtttttt tgtgatcttc tttgtttttc
      841 acctcatcag ccagcttgat caacagatcc gtaacttttt cgtccgagtc attgcgcttc
      901 cctggtgctg aaacgaccac gattctcctg tctgcatcat cttttacaat ctgcaatact
      961 tttttcaatt gagtcccatt cgcaagggaa ctcccgccaa atttaataac cttcaaaccg
     1021 aacacttcct cgcttgattt ctttaatgaa tttttaattt tgtaattgta ttttcattta
     1081 tgctttagaa tagcatcata tcacggctat ggcaattacc ttttcaaact ttacataatt
     1141 tgtaacatat ttgtattgtt aatactatgg ccatttgcaa aaaaaggaat atactatgag
     1201 ttaacttaat gtgggaggtt tcaaatcagt gaaaaaaaat aatccttgga ggatcgtcgt
     1261 cggattgtcc gtaatcgcaa gcatcagctc tgctttgttc aagtcaaatc gcaatgccgc
     1321 taaaagccca tcaacaacgt atacccctta catcggcacc tggaaaaacg acgcacagtc
     1381 cttgaaggtt gaaattactg ataatctgga aattctcctg aacaacaaaa aattaaaggc
     1441 gactctcgaa gaagccaagc ccaacgaatt ggtcttccgc gaccatttcg gctattattt
     1501 gattcttaaa aaagacacat cccctaccct gaccatctat gatgaggcgg aagaacaaga
     1561 attctacttc aaacgtgaaa tagataaata acgaatcgtt gacgcaacac cacgcccctg
     1621 atcagggctt tttttgtcgg gaattacacc gctaaaccac aagcaagggc taccccattt
     1681 ttcggggcag cccttgcttt tagttaatgt ttgatcagga ctgcggtttg atcttccaga
     1741 tttcgtctgc gtaacgcaga atcgtgtaat ccgatgaaaa cggccctgag cttgcgatat
     1801 tcatcaatgc catcctgttc catctgtctc tgtcggaaaa ggccacatcc gcctcatatt
     1861 gcgcttgtag gtaaccatcg aagtccttca aaaggaaata ctcatcattg tacatgacca
     1921 gcgaatcgaa gatgtcgcga ccttccgttt caacgccagg aatcgacccg tcaatgagca
     1981 gattcaggat ccgtttcaga cgcggattct tttcatagat ttcccttgaa ttgtatacgc
     2041 cattgcggta atactcttgc acctcatcgc tcttcaggcc aaagatgaag atattttcat
     2101 caccaacatt gtcccggatt tcgacgttgg ccccgtccaa agtagccatc gttacagcac
     2161 cgttcgacat cagtttcata ttgcttgtac cggaagcttc cttgccggca agagaaatct
     2221 gttcactgat atcagcagcc ggaatgatca attccgccaa ggataccccg tagttctcga
     2281 cgaaaacgat cttgatcttg tcattgatgg cagggtcgtt gttgacaaga tccgcaatcg
     2341 cgttgatcag tttgatgatt tgttttgcgt agtaataact tggggcagct ttagcaccga
     2401 aaatgaagac gcgcttctgc agatttgcct tcggatcatc cttcagttgc agatatctgt
     2461 ccagtatatg cagcacattc agtaattggc gcttataagc gtgcaatcgt ttgatctgca
     2521 cgtcgaacaa agcattcggg tcgatttcga tatgcatctc tttcagcaca taatccgcga
     2581 aacgtttttt gtttgccagt ttgacttgct ccagtttgtc cagaacaatt ttgttatcac
     2641 ggtgggcctt caaaactttg atttcttccg gatcgctctt ccattccgta ccgatcgtct
     2701 catcaagcat ttcggtcagg ttttcatttg cgatctgaac ccaacgcctt tgcgtgatgc
     2761 cgttcgtttt gttgttgaat ttttgcggat acatcacata gaaatcgtgc agcgtgtcag
     2821 ccatcagaat atccgagtgc aattttgcga cgccgttgac gctatggctg ccgatgatgg
     2881 cgagattggc catcttgatc tggccatttg cgatgatggc ggtgcgctgg gttaattctt
     2941 caccgtacaa tggcatcttt ttgtcgatat gacggcggtt gatttcctgg ataatctgat
     3001 agatacgcgg caacagatcc gcaatcatcg tttccggcca acgctccatg gcttcctgga
     3061 ggatcgtgtg gttggtataa ctgacagttt tcttcgtgat ttcccacgcc tgatcccaag
     3121 tcaactcttc ctcatccaac aggatccgca tcaattcagg gatcgctaaa gtcggatgcg
     3181 tgtcattgat atggatggcg attttatccg ggaaaaggct ccagtccagc tttaattgtt
     3241 tgaaatagcg gacaatgctt tggacgccgg cagaagagaa gaagtattcc tgttggacgc
     3301 gcaaaagtcg gccctcataa ttggaatcgt ccggatacag tacctcagtg atgcgtttga
     3361 cttcctcgcg ctgttcttct gtagaaaaac gtgcctcatc gccgtatgga atttccgctg
     3421 accaaaggcg cagattgttg atgattccgt tgttgtagcc tatttgagca gtatcgtaag
     3481 gaaccgccag gatatcccta gtgttttcgt gatgcacacg caacttgccg tccccatcct
     3541 gcgtaaaact gacatctccg ccgaaacgga cgatgacggc tttgttctct ttccgtactt
     3601 cccatacgtt ccgattgcgc agccagtttt ccggcaattc gatttggtaa ccgtttatga
     3661 atttttgctt gaaaagtcca taccggtagc ggataccgtt cccgttgccg gcaagtccgg
     3721 tcgatgcgat cgaatccatg aagcaggaag ccaatcttcc gaggccgccg ttccctaaag
     3781 ccggatcgac ttcagcgcgc gccacctcat ccaaatccaa cccaagctct tccaagcctt
     3841 gtttgacgca gtccattatc cccatgttca ggagattgct tttcagcatt cttccaggaa
     3901 ggaattccat ggaaaagtaa aagacttgtt tttctttttt cttgttgtaa tccgtcacgg
     3961 tttgaatcca gtcgtcagag tagaggcccc gcaccaaatc ccccagagcg atgtactgct
     4021 ccgttttcga acccgactca taaccgtaag cgaataattc ctcgaatttc ttctggaatt
     4081 ctttcttgaa ttcttgaaca tcaaatgcca tttacgtttg caactcctct tattcgttta
     4141 ctaatgactg ataaacatcc agataatctt tggccggttc tttccagctg aaatcctggc
     4201 tcatcgcttc gcgcaccatt tttgcccagg ctttcggctg attttcatat acgtccagcg
     4261 ctcggtagat ggttcccagc agtgcatacc cgctgaaatc aatgaaggtg aatccggttc
     4321 cttctcctgt gtaggaattg taagggatca ccgtgtcttt caaaccgccc gtttcatgta
     4381 cgatcggcaa agttccgtag cgcaacgaaa tcatctgcga taacccgcat ggttcgaaag
     4441 cggaaggcat caggaacaga tctgctcctg cgtacatctg ctgcgccaag gagatgtcaa
     4501 aatcgatgac cgccgcgaag cggtcaggat attttgcggc aaaataacgg aacgagttct
     4561 cgtattgctc ttcgccggtc ccgagaatgg caatctgcac atcgcgcgca ttcaacagtt
     4621 cctcggtttt ttcctgcaca agctgatagc ctttttggtc agtcaagcgt cctacactcg
     4681 tgattaatgg aatatccgga ttcacttcca gacccaaacg ttcctgcagg gctgctttat
     4741 tttttgcctt accggtcaaa tcatcggctg tgaaatggta ttcaagtttc ggatcggtct
     4801 ccggatcatt gacatcgtaa tcgattccgt tgatgatccc tctgattttc cagctgttgt
     4861 accccaaagt cccctccaag ccttcaccga attcctgtgt ctggatttca ttggcatagg
     4921 atgggctgac agttgtgaca acgtcagaga agttgatgcc accttt
//
LOCUS       DAOMUJ010000082         4957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4729_length_4957_cov_2.655039, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000082 DAOMUJ010000000
VERSION     DAOMUJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4729_length_4957_cov_2.655039"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..323
                     /locus_tag="PLT73_03250"
     CDS             78..323
                     /locus_tag="PLT73_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19431.1"
                     /translation="MTVSQRIEQSLYVFILLALAVAFVLTYALTKRIGRPIDAVMDVA
                     KKLSDHQYDARYKAKASVISKNWAKPSMNLPKVWIIK"
     gene            275..1360
                     /locus_tag="PLT73_03255"
     CDS             275..1360
                     /locus_tag="PLT73_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM19432.1"
                     /translation="MGETINELAESLDNQMNEIKQNDERLTGLINHLVIGVMLLDENK
                     EITMVNPVMQEILSESEESLIGQSYVEAVSSYGLSHMIERAYTYREPQNDEIYFYYPK
                     DRIVDVNIVPITSKKPGEMNLIVLLYDISEIRRLEKVRTDFVANASHELRTPVTALKG
                     FAETLLDGAMEDPVILKQFLEIILAESTRLNLLVNDILELSKLEQRQVPLALQQVNIS
                     EAVLATFQLVDQKVKEKNMTLELVEKDFVTLHTDPNRLKQILANLINNAVVYTQAGGH
                     ITVTVEKTADQVHLHVADNGIGIPEADLGRVFERFYRVDKARSRNSGGTGLGLSIVKY
                     LVENMNGTVTVASKQGNGTTFTVTLPI"
     gene            1579..2544
                     /locus_tag="PLT73_03260"
     CDS             1579..2544
                     /locus_tag="PLT73_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM19433.1"
                     /translation="MNFGKLSKFAMALTIGATLAACGNGDGTTAESGAETGSTTATGS
                     GTVLVDGSSTVFPIMEGVAEEFSIANPDVKATIGVSGTGGGFEKFIAGETDISNASRP
                     IKDEEIALLEEAGIEYTEFQLALDGLTVVVNSENDWVDQLTIEELAMIWTEGTGVETW
                     ADVREGWPEEKIELFSPGTDSGTYDYFDEVILDGEQINKAASLSEDDNVLVQGVSGSA
                     NAIGYFGYAYFLENSDIVKAVPIVNGEGVAVTPNDVTVQDGSYEPLSRPLFIYVKNEA
                     LENESVYEFTKYTLEMAAEMAAEVGYVALPAESYEAALTTLEGLK"
     gene            2631..>3295
                     /locus_tag="PLT73_03265"
     CDS             2631..>3295
                     /locus_tag="PLT73_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HRM19434.1"
                     /translation="MSKSVKDLMKEKSSRQKSLQFTEKAMPLFFLACAIISILTTFGI
                     VFTLAKETVTFFDEVPIREFLTGKEWYPFFKNDPSFGILPLLSGTFLIAGVAMLVAVP
                     IGLSSAIYLSEYATERTRKIVKPILEVLAGVPTVVYGLFALTFVTPLLQTVIPNLPIF
                     NALSPGIVVGIMIIPQITSLSEEAMGAVPNAMREGALALGATRLEVALKVILPGSLSG
                     IISS"
     assembly_gap    3296..3305
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3306..3561
                     /locus_tag="PLT73_03270"
     CDS             <3306..3561
                     /locus_tag="PLT73_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19435.1"
                     /translation="GISRAVGETMIVSTAAGSTPKFTLDVTESIQTMTSYIVQVSMGD
                     ATYGSTIYYSIYAVGMTLFLFTLLMNLLANYMSRRFREEY"
     gene            3561..4433
                     /gene="pstA"
                     /locus_tag="PLT73_03275"
     CDS             3561..4433
                     /gene="pstA"
                     /locus_tag="PLT73_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM19436.1"
                     /translation="MKLVEKNNSRINKRLLLNSVVKGLFFLATFFGIVVLAILVMRIV
                     SQGAAYLDWDFLSNYASRRPEDAGIKAAIYGTVWIMAIIAPLSLLLGVGTAIYLEEYA
                     PDNKFTHFIELNISNLSGVPSIVFGLLGLTVFVRLLEMGRSVLAGGLTMSLMILPVIV
                     VSSQEAIRAIPREQYEASYAMGATKWQTIRTVVLPAAVPGILTGSILALSRAVGETAP
                     LLMIGAMTFIAYIPESIWSGFTAMPIQIFNWAGRPQEEFQAVAAAGSIVLMLMLVIMN
                     SLAIYIRNKFSKRY"
     gene            4451..>4957
                     /locus_tag="PLT73_03280"
     CDS             4451..>4957
                     /locus_tag="PLT73_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19437.1"
                     /translation="MNPEVSLKDKKMIYSVKDFNLWYGNTHALKNIQLDIPTKEVTAI
                     IGPSGCGKSTFIKTLNRMVELVPIVKMTGSIIYNEQNIFDPRYKVEQLRTDVGMVFQK
                     PNPFPKSIYENIAYGPKIHGVKDKSVLDEVVEKSLKGAALWDEVKDRLKENAFGLSGG
                     QQQRLCIAR"
BASE COUNT         1370 a         1062 c         1264 g         1251 t
ORIGIN      
        1 ccgcggattg cagttcgtcc gatcgttcgc gttcctgatc cgcttcggcg cgcgccgcgg
       61 cgcgctcgcc cagcgccttg acggtcagcc agcgcatcga acagtccctt tacgttttta
      121 tcctgttggc tctggcagta gccttcgtac ttacttatgc tttgacgaaa cgcatcggca
      181 gaccgatcga tgcggtgatg gatgtcgcca aaaaactttc ggatcaccag tatgatgcgc
      241 gatataaggc aaaggcttcg gtgatttcca aaaattgggc gaaaccatca atgaacttgc
      301 cgaaagtctg gataatcaaa tgaatgaaat caagcagaat gatgaacggc ttactggctt
      361 gatcaatcat ctggtcattg gcgtgatgct tctggatgag aacaaggaaa ttacaatggt
      421 caatcccgtc atgcaagaaa tattgagtga aagtgaagaa tcgctgatcg gtcaatccta
      481 tgtcgaggct gtaagcagtt atggactcag ccatatgatt gagcgagcgt acacgtacag
      541 agagccgcag aatgacgaaa tctattttta ttatccaaaa gacagaatcg tcgatgtcaa
      601 tattgtcccg atcacaagca agaaaccggg tgaaatgaat ctgattgtgc tcctctatga
      661 cataagtgaa atccgacgct tggagaaagt ccgtaccgac tttgtcgcca atgcatccca
      721 tgaacttcgg acgcctgtta cagccctgaa aggattcgct gaaacattat tggacggtgc
      781 aatggaagat cccgttatcc tgaaacagtt tttggaaatc attctggccg aaagcacccg
      841 gttgaatctt ttggtgaatg atattttgga actgtcgaag ttggagcagc ggcaagtccc
      901 gctcgctctg cagcaagtga atatttccga agcggtgttg gcgacctttc agttggtcga
      961 tcagaaagtg aaagagaaaa acatgacact ggaattagtg gaaaaggatt tcgtaacgtt
     1021 gcatacagat ccgaatcgct tgaagcaaat actggctaat ctgatcaaca atgccgtcgt
     1081 ctatacgcaa gccggagggc atatcacggt cactgtcgag aagactgcgg atcaagtcca
     1141 tctgcacgtt gccgataacg gaatcgggat accggaagcc gatctgggaa gagtattcga
     1201 gcggttctat cgcgtcgata aagcgcggag cagaaactcc ggaggtaccg gcttaggatt
     1261 atcgattgtg aagtatttgg ttgaaaacat gaacggaacg gtcactgtag ccagcaaaca
     1321 gggcaatgga acaactttta cagtgacatt gcctatataa gaatggattc gaatgcaaaa
     1381 gctttacagg tggaaacgta cctgtagagc tttttttctt gtattctaaa gccccgttta
     1441 caaaacattt acaaaaaaag aggtaaatca acggaaaatt tacatgaaac cgcaaggaaa
     1501 ttaacaatcg accgttaata ttaaggttgt gaacaggcaa tggcgttcaa ataacctcaa
     1561 cataaaggat ggtaaaaaat gaactttggc aaattatcta aattcgcaat ggctttaaca
     1621 atcggcgcta ccttggcggc ttgtggtaac ggagacggca caactgcaga gagcggtgca
     1681 gaaacgggaa gcacgactgc aaccggaagc ggaacagttt tagtcgatgg atcttctacc
     1741 gtattcccga taatggaggg agtagcagaa gaattttcaa tcgctaatcc ggacgtaaaa
     1801 gcgactatcg gtgtatccgg tacgggtggc ggattcgaga aattcatcgc aggcgaaaca
     1861 gacatcagca acgcttcacg tccgatcaaa gacgaagaaa tcgcattgtt ggaagaagcc
     1921 ggcattgaat atacagagtt ccaacttgcc ttggacggtt tgacagtcgt tgtgaacagc
     1981 gagaacgact gggtggatca attgaccatc gaagaattag cgatgatctg gacagaagga
     2041 accggcgtag aaacatgggc ggatgtccgt gaaggctggc cggaagaaaa gatcgagttg
     2101 ttcagcccag gtaccgactc aggcacatac gattactttg atgaagtgat cttggacggc
     2161 gaacaaatca acaaagcagc ttctttatcc gaagatgaca acgtattggt acaaggtgtt
     2221 tccggttccg caaatgccat cggctacttc ggttacgcat acttcctgga aaacagtgac
     2281 atcgtcaagg ctgttccgat cgtgaacggt gagggcgttg cagttacgcc taacgatgtg
     2341 accgttcaag atggatcgta tgagccgctt tctcgcccgt tgttcatcta cgtgaagaac
     2401 gaagcgttgg aaaacgagag cgtctacgaa ttcacaaaat acacattgga aatggcagcc
     2461 gaaatggcag ctgaagtcgg ttatgtagca ctaccggctg aatcttatga agcagcattg
     2521 actacgcttg aaggtttgaa ataagcaaaa tcaaataaat gactaaggga tgtgagggaa
     2581 gagagcgttt tgcttccctc gcatctcatc tttattagag gagaatcaat atgtccaaat
     2641 ctgtcaaaga cttgatgaaa gaaaaaagtt cacgacaaaa atctttgcaa ttcacagaaa
     2701 aagccatgcc gctttttttt ctggcttgtg cgatcatttc gatcctgacc acgttcggaa
     2761 tcgtgttcac actggccaaa gaaacagtca catttttcga tgaggttccg attcgggaat
     2821 tcctgacagg aaaagagtgg tatcctttct tcaaaaacga tcccagtttt gggattttgc
     2881 cgctattgag tggaaccttc ctgatagccg gcgttgcgat gcttgttgcc gtaccgatcg
     2941 gcttgagctc agccatctat ttgagcgaat atgcgaccga acggacaaga aaaatcgtca
     3001 agccgattct ggaggtattg gccggagttc ctacggtggt atacggactg ttcgccttga
     3061 cgttcgtcac tccgttgctg cagacggtta tcccgaacct gccgatattc aatgcgctta
     3121 gccctggtat tgttgtggga atcatgatca tcccgcagat cacgtcctta tcggaagagg
     3181 cgatgggtgc agtgccgaac gcgatgcgtg agggggcttt agctttaggg gcaacccgtt
     3241 tggaagtggc gctgaaagtc attttgccgg gatccttgtc tggtatcatt tcatcnnnnn
     3301 nnnnngggta tttctcgtgc agtgggagaa acgatgatcg tatccacagc cgctggatcc
     3361 acccctaaat ttacgcttga tgtaacagaa tcgatccaga cgatgacatc ctatatcgtt
     3421 caggtcagca tgggggatgc cacctatggt tctacgattt attacagtat ctatgcggta
     3481 gggatgacgt tgttcctctt caccttgttg atgaacttgt tggcgaatta catgtcccgt
     3541 cgttttaggg aggaatattg atgaaattag tggaaaagaa caacagcaga atcaataagc
     3601 gactattgct gaacagcgtg gtcaaaggac tgttttttct ggcaaccttc ttcggaatcg
     3661 ttgttttggc cattctggtc atgcggatcg tgtcgcaggg cgcggcttat cttgattggg
     3721 atttcttgag caactatgct tcgagaagac cggaagatgc cggcatcaag gctgccatct
     3781 atggaacggt ctggatcatg gccatcatcg ctcccttgtc actccttttg ggtgtcggaa
     3841 cggcgattta ccttgaagaa tacgcgcccg acaataaatt tacgcatttc atcgagttga
     3901 acatttcgaa tctttccggt gtaccatcga ttgttttcgg gctgttggga ctgactgtgt
     3961 ttgtccgttt gttggaaatg ggccggagtg tcttggctgg tggtctgacc atgagcttga
     4021 tgatattgcc tgtcatcgtg gtttccagcc aagaagccat ccgtgccatc ccgagagagc
     4081 aatatgaagc ctcttatgcg atgggtgcca cgaaatggca gacgattcgg acggtcgttc
     4141 tgccggcagc agtgcctggc atcctgaccg gttcgatatt ggcgctatca agggctgtcg
     4201 gagagactgc accgttgttg atgatcggag cgatgacctt tatcgcttat atcccggaga
     4261 gtatctggtc cgggtttacg gcaatgccga tccaaatctt caactgggca ggtcgtcccc
     4321 aagaagagtt ccaggctgtc gctgcagccg gaagtattgt cctaatgctt atgttggtca
     4381 tcatgaactc attggcgatc tacatccgca acaagttttc gaagcgttat taaattagga
     4441 ggttactcat ttgaacccag aagtgagctt aaaagataaa aaaatgatct atagtgtgaa
     4501 agatttcaat ctatggtacg gaaataccca tgccttgaag aacatccaac tagatattcc
     4561 cactaaggaa gtcacagcga tcatcggacc atcaggctgc ggaaagtcca ctttcatcaa
     4621 gaccttaaat cggatggtag aattggttcc aatcgtaaaa atgacgggat caatcattta
     4681 caatgaacag aatatttttg atccgcggta caaagtggag cagttgcgta cagatgtggg
     4741 catggttttc caaaagccga accctttccc gaaatcaatc tatgaaaata tcgcctacgg
     4801 accaaaaatc catggcgtaa aagacaaaag cgtactggat gaagtcgttg agaaaagctt
     4861 gaaaggcgca gcgttgtggg atgaagtaaa agatcgtctg aaagaaaatg ccttcgggtt
     4921 atctggcgga caacaacagc gtttgtgcat cgccaga
//
LOCUS       DAOMUJ010000083         4940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4746_length_4940_cov_1.700102, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000083 DAOMUJ010000000
VERSION     DAOMUJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4746_length_4940_cov_1.700102"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2443)
                     /locus_tag="PLT73_03285"
     CDS             complement(<1..2443)
                     /locus_tag="PLT73_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRM19438.1"
                     /translation="MDTEQGYIVGEIKAIFFENASNFYKVMLISVNESNLPQKQDEIV
                     VTGSFGQITEDTAYRFFGEVVEHPRYGVQFQATSYQQEKPTSKNGLIAFLSGERFPGI
                     GKRTAEKIVETFGEEAVDVILDDPEALKSISGMTPKKREMMRDVLMQTQGTEKILIAL
                     GNYGFSNNQAANIFHFYRTETLTVLNENPYRLLEDIEGIGFKKADQLAEELNFAPDHS
                     SRLKGGLIATLQELCLSNGDTYVDGTVLLERTIHLLEQSRRFIIEDAPVIEALMEMVM
                     DMKVVEDHSRFAIPSLYFAEEGIASSIDRLLKRKTKIAYPGVNLDLEIENLQSNLQIR
                     YGASQIEAIKAALLSPFFILTGGPGTGKTTVLKGIVRMFSELNDLPEDPKDYKDGLFP
                     ILLAAPTGRAAKRMQETTGLPGSTIHRLLGLTGQEKESEELYTQELEGKLLIIDEVSM
                     VDTWLMHRLLKSVPPGMQVLFVGDKDQLPSVGPGQVLFDLLNCKPLPQVELNEIFRQS
                     GDSSIIPLAHEIKNGILPRDFRNNQADRSFLPCQTHQIEPVIRQVVEKAKSKGFTAKD
                     IQILAPMYKGAAGIDAINTMMQEIFNPKGNKKRREVAFFDVVYRVGDKVLQLVNQPEN
                     NVFNGDMGEITAIQFAKETEEKVDQITILFDTVEVTYNRNNWNKFVLAYCCSIHKSQG
                     SEFTMVILPMVKQYGRMLRRNLLYTAITRSKSKLILCGDYEAFETAVVSTGDIRKTML
                     HEKLERNLNNDKVFTAEESAKDKEAKAPGTGIEVAEPITETTAEPATQKPEKNASPNI
                     LPVYQLTIEQISNGSI"
     gene            complement(2572..3696)
                     /gene="mnmA"
                     /locus_tag="PLT73_03290"
     CDS             complement(2572..3696)
                     /gene="mnmA"
                     /locus_tag="PLT73_03290"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRM19439.1"
                     /translation="MQDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DEFGFCTATEDYKDVQAVAQQIGIPYYSVNFEKEYWDKVFQYFLDEYKKGRTPNPDVM
                     CNKEIKFKAFLDYAMELGADYVATGHYAQVVRDEDGTAHLLRGVDDNKDQTYFLNQLS
                     QEQLSKTMFPLGGIKKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKEFLTNFLPAI
                     PGKMVTLSGDVMGEHAGLMYYTIGQRKGLGIGGGGESDDPWFVIGKDLATNTLYVGQG
                     YHHENLYADSLDATDIHFTSEGKKPSVFTCTAKFRYRQQDTAVTVYLNEDQTTARVEF
                     AEPVRAITPGQAVVFYDGMECLGGGTIDKAYQADRVLQYI"
     gene            complement(3834..4424)
                     /locus_tag="PLT73_03295"
     CDS             complement(3834..4424)
                     /locus_tag="PLT73_03295"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRM19440.1"
                     /translation="MYNNKTALIKWIAILTMTIDHIGYYLFPSLLFLRVVGRIAFPCF
                     LYTTVQGVRETHDFRKYLLRLVVTGLVSMPITAQTGNVFNILFTLALFALSIKDYRFI
                     LPAIFLVQFTEYGLYGFLMGWTIYVLSEKNVQAGIVLFLLVNALQFPSLQGFAALAFI
                     PLLLPVTFPVRPLPKWAGYAYYPIHQVVLIFIAKLM"
     assembly_gap    4668..4717
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1178 a         1255 c         1097 g         1360 t
ORIGIN      
        1 cgatgctccc gttggagatc tgttcgatcg tcaattggta gacgggtaat atatttgggg
       61 acgcattctt ttctggcttt tgggttgcgg gttctgcagt tgtttcggtg atcggttcag
      121 caacttcaat cccggtaccg ggcgccttcg cttccttgtc ctttgcagac tcttccgcag
      181 tgaagacctt gtcattgttc aggttgcgct ccagtttttc atgcagcatc gttttccgga
      241 tatcgccagt gcttacgact gccgtttcga aagcttcata atccccgcac aaaatcaatt
      301 tgcttttgct cctggtgatg gctgtgtaaa gcagattgcg gcgcagcatc cgcccgtact
      361 gtttcaccat cggcagaatg accatcgtaa attcgctgcc ctgcgattta tggatggaac
      421 aacagtaagc caaaacaaac ttattccaat tgtttcgatt gtaagtgact tccacggtat
      481 cgaacaggat cgtgatctgg tcgaccttct cttccgtttc cttggcgaat tggatggcag
      541 tgatttcacc catatcccca ttaaagacat tattttccgg ttggttcacc aactgaagca
      601 ccttgtcgcc tacccgatag acgacatcaa aaaaagcgac ttcccgtctt ttcttgttcc
      661 cttttggatt gaagatctcc tgcatcatcg tattgatggc atcaatgccg gcagctcctt
      721 tgtacatcgg cgctagaatc tggatatcct ttgcagtgaa cccttttgac ttcgcctttt
      781 cgacgacttg ccggatcacc ggctcgatct gatgcgtttg gcaaggcaga aacgagcggt
      841 ccgcttgatt gttgcggaaa tcgcgcggga ggatgccgtt cttgatttcg tgcgccaacg
      901 ggatgatcga tgaatcgccg gattgcctga atatctcatt cagctcgacc tgcggtaacg
      961 gtttgcagtt cagaagatcg aacaacacct ggcctggacc gactgaaggc aattgatcct
     1021 tatcccccac aaacagcact tgcataccag gcggaacgga tttcaagagt cgatgcatca
     1081 gccacgtatc caccatcgaa acttcgtcga tgatcaacag cttgccttcc aactcttgcg
     1141 tataaagttc ttcggattcc ttttcctggc ccgtcaaacc cagcaagcga tggatcgtgc
     1201 tgccgggcag acctgtcgtc tcctgcatcc tttttgcggc acggccggtc ggagcggcaa
     1261 gcaaaatcgg gaaaagtccg tctttgtaat ccttagggtc ttccggcaga tcgttcaatt
     1321 cggagaacat gcgcacgatc cctttgagaa cggtcgtttt accagtaccg ggtccacctg
     1381 tcaggatgaa gaacggtgaa agcaaagctg cttttatcgc ttcgatttgg gaggccccat
     1441 agcggatctg cagattggat tgcagattct cgatttccag atccaaattc actcccggat
     1501 aagcaatttt tgttttccgt ttcagcagtc tgtcgatcga tgatgcgatg ccttcttcgg
     1561 cgaaataaag ggacgggatg gcaaagcggc tgtgatcctc gaccactttc atgtccatga
     1621 ccatttccat caacgcttcg attactggtg catcttcgat aatgaagcgc cggctttgtt
     1681 caagcaagtg gattgttcgc tcaagcaaga cggtcccatc gacataggta tctccgttcg
     1741 acaggcaaag ttcctgcagc gtcgctatca ggccaccttt cagacggctg ctgtgatccg
     1801 gagcgaagtt caactcctcc gccagctgat cggctttctt gaagccgatg ccttcgatat
     1861 cctcgagcag tcggtaagga ttttcattta ggacagtcag cgtttcagtg cggtagaaat
     1921 gaaagatatt cgctgcctga ttattggaga agccatagtt acccaatgcg atcaggatct
     1981 tctcggttcc ttgcgtctgc atcagtacat cgcgcatcat ttcccttttt ttgggcgtca
     2041 tgccgctgat gcttttcaga gcttccgggt catcaaggat gacgtcgacc gcttcctcgc
     2101 caaaagtctc gacgattttt tcagctgtcc gtttaccgat tcctgggaaa cgttcccccg
     2161 ataagaatgc gatcaacccg ttcttggagg ttggtttttc ctgctgatag gaagtggctt
     2221 ggaattggac accgtatctt gggtgctcca cgacctcacc gaaaaagcgg taagctgtat
     2281 cttcggttat ctgcccgaag cttcccgtca caacaatttc atcctgcttt tgcgggagat
     2341 tcgattcatt gactgaaatc agcatgactt tgtagaaatt actcgcgttt tcaaagaaaa
     2401 tcgctttgat ttctccgacg atatagcctt gttctgtatc catccggatg atcccctccc
     2461 ttttttgact attcagattt gttataagat tccttaagaa cgataccaca attcgcggcc
     2521 aaattaaaga gaggccggag aaaaaaccgg cctctcgctt attcttttcc tctaaatata
     2581 ctgcaggaca cggtcggctt gatatgcctt gtcgattgtg cctccaccta agcattccat
     2641 cccatcgtaa aacacaacag cttggccagg tgtgatggca cggaccggtt ccgcaaattc
     2701 tacacgagcg gtcgtttggt cttcgttcag atagacagtg actgccgtgt cttgttggcg
     2761 gtagcggaat ttagccgtac acgtgaagac ggatggcttc ttgccttcgg atgtgaaatg
     2821 gatatcggtc gcatcaaggc tgtccgcata cagattctca tgatggtaac cttggcccac
     2881 atagagcgtg ttggtcgcca gatccttgcc gataacaaac catggatcat cggattctcc
     2941 gcccccgcca attcccagac cttttctttg cccgattgta taatacatca atcccgcatg
     3001 ctcacccatc acatcgccgc tcaatgtgac cattttcccc ggaattgccg gcagaaaatt
     3061 cgtcaagaat tctttgaaat tcttctcgcc gatgaaacag atgcctgtag aatctttttt
     3121 cttcgctgtc gccaagcctg cttcttcagc aatgcggcgc acttccggct ttttgattcc
     3181 gcccagcgga aacatcgttt tggatagttg ctcttgtgat agctggttca ggaaataggt
     3241 ttggtccttg ttgtcgtcca cgcctctgag cagatgagcg gtgccgtctt catcacggac
     3301 gacttgcgca tagtgccctg ttgcgacata atccgcaccc agttccatcg cataatccaa
     3361 gaaagctttg aatttgattt ccttgttgca catgacatcc ggattcggcg tccggccctt
     3421 cttatattcg tccaggaaat attggaagac cttatcccag tattcctttt cgaaattgac
     3481 ggaataataa ggaatgccga tctgttgtgc gacagcctga acatccttgt aatcttccgt
     3541 tgccgtacag aatccgaatt catccgtatc atcccaattc ttcatgaaaa tgccgatgac
     3601 gtcgtaacct tgttccttca aaagcaatgc tgtgacagaa gagtcaacac caccgctcat
     3661 tccgaccacc acgcgtattt ttgaattgtc ttgcatgcct atcacaccct tacttgattt
     3721 gtgtattttc tcacttctta cattatataa gtaacatccg atacaaagaa acagtttaca
     3781 ccatcctggc gcatatttca agctttcttg cctatccgta aacaagctga ctgtcacatc
     3841 agcttagcga taaatatcag tacaacttga tggatgggat agtaggcata cccggcccat
     3901 ttcggcaacg gtctgactgg gaaagtgacc ggcagaagca acggaatgaa cgccaaggca
     3961 gcgaacccct gcaatgatgg gaattgcaga gcattcacca ataaaaacaa aacaattcct
     4021 gcttgtacat ttttttccga cagcacatag attgtccagc ccatcaaaaa gccatacaag
     4081 ccgtattccg taaactggac caaaaaaatg gccggcaaga tgaaacggta gtctttgatc
     4141 gataaggcaa aaagtgccaa agtgaacagg atattgaaga cattccctgt ttgggcagtt
     4201 atcggcatac tgacgagccc cgtcacgaca agccgcagca gatacttacg gaaatcatgc
     4261 gtttccctta ccccttgcac ggttgtgtac aaaaaacaag ggaaagcgat acgtccgaca
     4321 acccgcagaa aaagcagtga ggggaacaga tagtagccga tatggtcgat cgtcattgtg
     4381 aggatcgcaa tccacttgat cagtgccgtt ttgttattgt acactattcc gtcacttcct
     4441 ttttattcat tttcatcaag ctgcaaaatt actttcgaaa acaaagaaaa caaagcagaa
     4501 aaccttggaa ggatttctgc ttcatcgtta ggagcgccat cgatcaaact gtataaggtt
     4561 ttgcatccag attcagatgg gccaatgctt gctcgatgtc ttcgatgatg tcatccacgt
     4621 tttcaagccc taccgaaaaa cggatcaggc ctggcgtgat gcctgctnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact tgcaggatga agcacacaag
     4741 tccggatatc ggccacatgc acttcattgg acgccaactt cagactgtcc atgaatttga
     4801 tggcattttc tctgctgcct tcgatggaga gcgaaatgac gccgcacgtt ccgagcggca
     4861 aatatttatt cgccaattcg tggtacttgt ctcctctaag cccggataat tcacaaattc
     4921 aactttttcg gattttttga
//
LOCUS       DAOMUJ010000084         4925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4770_length_4925_cov_1.997331, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000084 DAOMUJ010000000
VERSION     DAOMUJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4770_length_4925_cov_1.997331"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..451)
                     /locus_tag="PLT73_03300"
     CDS             complement(146..451)
                     /locus_tag="PLT73_03300"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM19441.1"
                     /translation="MSKLDVKTKSAGVKFDDIKAQMMEDAEFHEEYDKLQPRYELISQ
                     IIEARKKMKMTQEELAKRAGTRKSNISRLESGSYNPSLDFLIKIAKGLGKDVHIEIR"
     assembly_gap    1068..1081
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1334..2038
                     /locus_tag="PLT73_03305"
     CDS             1334..2038
                     /locus_tag="PLT73_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM19442.1"
                     /translation="MDKVQELLDDLYLVCQPIVLCSRANVIDGYEILLRSTKLRAFPE
                     KMFLWFIEKDERNARLMAYYFRELKKLVDRHSNTNLALNLHPKQLQHPSTWTFLDNIS
                     SMKGNVSIELTEHCCEFDPADGADALQTYISNMKSKGFSLAIDDIGSGQNNFELISKN
                     VQNIHTIKISLLDFRNTNEDSMFFSLNNWLNFSNRYNLKMVVEGVESECISNKLKNLG
                     MVYQQGYYHGKGQVLC"
     gene            2602..3882
                     /locus_tag="PLT73_03310"
     CDS             2602..3882
                     /locus_tag="PLT73_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM19443.1"
                     /translation="MTMLHQFTFKNFKSFKEETTLDMLASSIKEHPHDVVTDVMEEKV
                     LKVAAIYGANASGKSNVIEAFATMKNLVLYSFRQNVFSTKNDIEPYWFEDKNVPTEFN
                     VIFSTEGDIYQYGFSVLEGVILEEYLYQRDPKVKKETYIELFSKNGETLSGELLGNIG
                     TQNILELIEEDTLLISVISKLKIPFAQNVFDWFKEVRIIDYGNPRKELGLPHFTRGNV
                     IGNPLLKLLENEAEKKKLEKFIQAIDVGIDKLGIVQDILNEDSLSERSTRELKRIVTY
                     HKNPKTKELLMTPLESESSGTLKMLTLYVDLKNILDSGGTIFVDELDAKLHPLLIRYI
                     IIMFHDEKMNPNHAQLIFSTQEIFTLDKDNFRRDEIWFADKNDEGVSELYSLADYVDD
                     EEKKVRNDASYGKDYILGRYKSIPTLRRIEDIDG"
     gene            3875..4555
                     /locus_tag="PLT73_03315"
     CDS             3875..4555
                     /locus_tag="PLT73_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRM19444.1"
                     /translation="MAKKIGKKQRKAKRIPEPASYFIGSEGTKTEVLYFKWIADRLEK
                     QYSGFQGRIEVPSFHIKGIGSSNLRLLDDIEEYLKNDPMLYENVWAVFDKDDVPLAYF
                     DNAITVAANKGWHTAWSNDSFELWYLLHFEYLQSGISREQYKQKLTAHCNKVGIAKYE
                     KKDPQLIEALYPLSSVAIRNATRLEKNYAVWMPSSQRLPCTTVHHLVQELINLEANVE
                     QLRQQNNS"
BASE COUNT         1375 a         1082 c         1118 g         1336 t
ORIGIN      
        1 caactccggc tagcgtttgc tagtcggagt tgagcttgtt atgcgggtgc ttagcttcgg
       61 ttggagtagg cctcgtcggt gttgggatgc caccgattta attcttttat ttttttgctg
      121 tagactacgc aatcaaaaac aatcatcaac gaatttcgat gtgcacatct tttcccaatc
      181 ccttagctat tttgatgagg aagtctaatg aagggttgta agaaccactc tcgagtctgg
      241 aaatgttgga ctttctggtt ccagctcttt tagccagctc ttcttgagtc atcttcatct
      301 tttttcttgc ttcgatgatt tgtgaaatca actcgtatct tggctgtagc ttgtcgtatt
      361 cctcatgaaa ttcagcatct tccatcattt gcgctttaat gtcgtcaaac tttactcctg
      421 cagactttgt tttaacatcc aatttactca tcactacacc ttctttcata atcttcttta
      481 gaaactcctc agtaaaatat tatcatatac gataacattt tactcaaatg atgaattgtg
      541 tttacagtag cgcaacaaaa tgggcagaca tcacataaat acctctaact cttcttaaac
      601 agatgaatgt agctactgac gacacctgac actttcgaac gacgagcaga ttctctgccg
      661 gaggagaaca tcaaaaaaga tgattacctc cgaccaacgc ggcttcgcag gagttcggcc
      721 gaccgccaca catgctcctc cggccaacat gcgctcaccg gtgttgagat cgccagcaca
      781 gtctgtcttc cgacgagcgg ggcgctcgtc ggagctgacg gtttcctccg gttagggggt
      841 tccttgccgg aggaaatcgc ccaaaaagat catcacctcc gaccacggcg gcttcgccgg
      901 agttcggcct ccgcaccacg cagcctcctc cggcgaacat tcgctcacag gagttgagtt
      961 ggccggcgca gtctgtcttc cgacgagcgg ggcgctcgtc ggagctgacg atttcctccg
     1021 attaaggttc tttcggttat tctttttgga ctattttgat gcaacggnnn nnnnnnnnnn
     1081 ncacaggatc aacgcacaat cccaaagatg cggtccaaat caggttcgcc gcatgggagc
     1141 gcagagggct ttcttcaaac aaaggaaagc acaggtgcga tgaggttgtg tcttcgagcg
     1201 tgatgatttt tggcacgatt gatttttttg cacggatata ttcgaataat cagccgatat
     1261 gatttatttc aaaaggattc ggctccatat agtgtgacag tttggtttgc agtgatcgga
     1321 aggggaaaac gggatggata aggtgcagga gttgttggat gatttatact tggtgtgtca
     1381 gccgatcgtg ctgtgttcgc gcgcgaatgt tatcgatggg tacgaaattt tattgagatc
     1441 cacaaaattg cgggctttcc ctgaaaaaat gttcctgtgg ttcattgaaa aggacgaacg
     1501 caatgccagg ttgatggctt attattttcg ggaactgaaa aaattagtgg acaggcacag
     1561 caacaccaat ctggcgctga atctgcatcc aaagcagttg cagcatcctt ccacgtggac
     1621 ttttttggat aatatttctt cgatgaaagg gaatgtctcg atcgaattga cggagcattg
     1681 ctgcgaattt gacccggccg atggagctga tgccctccaa acctatatct ccaatatgaa
     1741 gagcaagggt ttttcattgg cgatcgatga tatcggcagt ggccaaaata actttgaatt
     1801 gatctcaaaa aatgttcaaa atattcatac catcaaaatt tcgctgctgg atttcagaaa
     1861 caccaacgaa gacagcatgt tcttttcctt gaataattgg ctcaactttt ccaaccgcta
     1921 caatctgaaa atggttgtcg aaggcgtcga gtcggaatgc atcagcaaca agctgaaaaa
     1981 cctgggtatg gtctatcagc aaggctatta ccacgggaaa ggccaagttt tgtgctgaac
     2041 ttttgcggcc ggagtagccg gccaaacatt tttcatcgtt agtccaatcc ggatgcgccg
     2101 ggttgggcta tttttttgtg cccgttcctc cggttaaggg gttgtctgcc ggaggaggag
     2161 atggataaag atgagcacct ccgatcaccg cggcttcgcc ggagttcagc cacccgcaat
     2221 gcatcctcct ccggcgaaca ttcgctcaca ggagttgaga tcgccggcac agtctgtctt
     2281 ccgatgagcg cctggctcat cggagctggc agtttcctcc ggttaggggg ttccttgccg
     2341 gaggaaatcg cccaaaaaaa tcatcacctc cgaccacaac ggcttcgccg gagttcggct
     2401 gtccgccgcg attcctcctc cggcgaacat tctctcgcag gagctgagct cgctggcaca
     2461 ccaccacttc cgatgagcgg accgctcatc ggagctgagg tgctgtcatc tactgattga
     2521 ttcaccaatg gttgcgaatt tgagaaattt tctcaaatat ttgctcggcg catatattta
     2581 ggttatgata tggatgaggt gatgacgatg ctacaccaat ttacgttcaa aaattttaaa
     2641 tctttcaaag aagaaactac actggatatg ctggcttctt ccatcaagga gcatccacat
     2701 gatgtcgtga cggatgtgat ggaagaaaaa gtattgaaag ttgcagcgat ttatggtgca
     2761 aatgcttccg gtaagagcaa tgttattgaa gcgtttgcca ctatgaaaaa tctggtgctt
     2821 tattccttca gacaaaatgt tttttcgaca aagaatgata ttgagcctta ttggtttgaa
     2881 gataagaatg tgcctacgga gttcaatgtc attttttcta cggaaggcga tatctatcaa
     2941 tatggatttt ctgtattaga gggcgttatt cttgaagaat atctttatca gagggatccg
     3001 aaagtaaaaa aagaaaccta tattgaacta ttcagtaaaa acggtgagac tttatcgggg
     3061 gaactgctcg gtaatatagg aacccagaat atcctggagc tcattgaaga agacactttg
     3121 ctgatttctg tgatatccaa attaaagatt ccatttgctc aaaatgtttt tgattggttc
     3181 aaagaggtga gaatcattga ttatggaaat cctagaaagg aactaggact tccgcacttt
     3241 actcgaggaa atgtaatcgg aaatccactg ctcaaactat tggagaatga agcagagaaa
     3301 aagaaactgg aaaaatttat ccaagccatt gatgtgggca ttgataaact ggggattgtc
     3361 caagatatat taaatgagga ttctctatct gagcgaagca ccagagaatt gaaacgcatc
     3421 gtgacctacc ataaaaatcc caaaacgaag gaattactta tgaccccgct tgagagtgaa
     3481 tccagtggga cattgaaaat gctaacgtta tatgtggatt taaaaaatat tcttgatagc
     3541 ggtggtacta tttttgttga tgaattggat gcgaagctgc atccattgtt gatccgttat
     3601 atcatcatca tgtttcatga tgaaaagatg aatccaaacc acgcacaact gatcttttca
     3661 acgcaggaaa tatttaccct tgataaggat aatttccgaa gagacgaaat ttggttcgct
     3721 gacaaaaatg acgaaggagt ttctgaactc tactcattgg ctgattatgt ggacgatgag
     3781 gaaaagaaag tccgcaatga tgccagttac gggaaagatt atattttagg aagatataaa
     3841 tccattccga ccttgcgtcg aatcgaggat atcgatggct aagaaaatcg gaaagaagca
     3901 acggaaagca aaacgaattc cggaacccgc cagttatttc atcggatcgg aaggaacgaa
     3961 aacagaagtc ctttatttta aatggattgc cgatagattg gaaaaacaat attcagggtt
     4021 ccaaggcaga atcgaagtcc cctcttttca catcaaagga atcgggtcca gcaatctacg
     4081 cctgttggat gatatagaag aatacctgaa aaacgatccc atgctttacg aaaatgtctg
     4141 ggcggtgttc gataaagatg atgtcccgct cgcctatttc gataacgcca ttactgttgc
     4201 cgcgaataag ggttggcata cagcttggtc taacgactct ttcgaactat ggtatctctt
     4261 gcattttgaa tacctgcagt ctgggatatc gagggaacaa tataagcaaa aactcactgc
     4321 tcattgcaac aaagttggca tcgctaaata cgaaaaaaaa gacccacagc tgattgaggc
     4381 tttataccca ttaagcagtg tggccatcag aaatgctact cgcttggaaa aaaattatgc
     4441 ggtctggatg ccatcttcac aacgtctccc ctgtacaacc gttcaccatt tagttcaaga
     4501 attgataaat ttggaagcaa acgttgaaca attaagacag caaaataatt catagacttc
     4561 aaatcatcta gcgtattctg aaatttgaat agcccactac tatatcagtt aaatagtagt
     4621 gggctattta ctgtcaaata aatagtagtt tccgctgccg gaggaggaga taaaaaagat
     4681 cgtcacctcc ggcaaccgcg gcttcgccga acttcagcca ccagccacga ttcctcctcc
     4741 ggcgaacatt cgctcacagg agttaatctc gctggcacat ccccacttcc gatgagcgca
     4801 ctgctcgtcg gagctggcag tttcctcctg taaaggggta ctctgccgga ggagcacatc
     4861 cgaaaaaact atcacctccg accactgcgg cttcgccgga gttcacccgc ccaccacgcc
     4921 ctctc
//
LOCUS       DAOMUJ010000085         4892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4817_length_4892_cov_2.497002, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000085 DAOMUJ010000000
VERSION     DAOMUJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4817_length_4892_cov_2.497002"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLT73_03320"
     CDS             complement(<1..960)
                     /locus_tag="PLT73_03320"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM19445.1"
                     /translation="MSKKNIVVVGAGFAGVAAAKKLSRHFKKNPDVLITLIDRHSYQT
                     YMTELHEVAAGRVQPDAIQYDLQRLFSRNRNVDIVTDEVTHVDREKKVVTTSSHSFNY
                     DYLILAMGGEPNTFNVPGVDEHAFTMWSWEDANKIRRHIKDTVEAAAKEHDDAKRKAM
                     LTAVVSGAGFTGVELVGELMEWKDKLAKDNKLDPAEFSLYLVEAAPQILGVVTEKEQQ
                     KAEKYMLKKGIQIIKGNGVSNVKEDSVELSDGTVIPTHTLIWTAGVKANSDAAAYGIE
                     QARAGRLVANKYMEAKDSDGVYLAGDLVYYEEPDKNNAPVPQIV"
     gene            complement(1189..1776)
                     /locus_tag="PLT73_03325"
                     /pseudo
     CDS             complement(1189..1776)
                     /locus_tag="PLT73_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
     gene            complement(2159..2764)
                     /locus_tag="PLT73_03330"
     CDS             complement(2159..2764)
                     /locus_tag="PLT73_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM19446.1"
                     /translation="MNEEVELSEYSPEQLVALIQQGHSMHFEELFYRFLPLVKKFNRA
                     YYLKSLEQDDFWQEARMVLHKAVQSYVPEKGLQFASFYKLTLKHHIFSLIRKESAVKR
                     RIDKGAVSLDAILENQYSNHTEHSFEGVVSMSFSPEEIVMVKESASGYFESLSDFEQA
                     VFIRFLNGSDFLSIADELDCEVTSIKNAYDRCHRKMKRLLE"
     gene            3511..4365
                     /locus_tag="PLT73_03335"
     CDS             3511..4365
                     /locus_tag="PLT73_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM19447.1"
                     /translation="MKYTTLNDNAIMPTLGIGTYRIRPTDAERSVEHALKNGYQLIDT
                     ANIYLNEKAVGRAIKKSGIDRSEIFLTSKIWPTEYKYAKAKQAIEVTLKRLGVDYIDL
                     MLLHQPTGAYLGAWRAMEEAVKTGKIKSIGLSNFTGLELDKVLAEGTIKPAVVQVECH
                     PYFQQKDLKAKLAKDNIALEAWYPLGSADKDLLAEPIFAELASKYNKSVVQIILHWHV
                     QTGNIVIPGSKNPTHIDSNLAIFDFELSSADLEQIAQLDKDKRFLNLPKWMHRLFLLA
                     RLNYDKQV"
     assembly_gap    4438..4447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4672..>4892
                     /locus_tag="PLT73_03340"
     CDS             4672..>4892
                     /locus_tag="PLT73_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19448.1"
                     /translation="MKKPNLMLLVFTLMSFVLAMTAFVFSGILDKVAVSLDISVAQSG
                     LLNTMYSYGAAFGVPITLILFRKVERSKML"
BASE COUNT         1334 a         1202 c         1009 g         1337 t
ORIGIN      
        1 cacgatttgc ggtaccggcg cattgttttt gtcaggctct tcgtagtaga ccaagtcccc
       61 cgccaagtaa acaccatcgg aatctttcgc ttccatgtat ttattggcta ccaaacgacc
      121 cgctcttgct tgctcgatgc cgtaagctgc tgcatcagag ttggctttta cccctgccgt
      181 ccagatcaac gtgtgtgtcg gaatgaccgt tccatcagac aactcgacgc tgtcctcctt
      241 gacgtttgag accccattgc ctttgatgat ctggatgcct tttttcagca tgtatttttc
      301 ggctttttgt tgttcctttt cggtaacgac acctaaaatc tgtggtgcgg cttccactaa
      361 ataaagactg aactcagccg gatccagctt attgtcttta gccagtttgt ctttccattc
      421 catcagttcg ccaaccaatt cgacaccggt gaagccggca ccactgacta cagcagtcaa
      481 catggccttg cgcttcgcgt catcatgttc cttcgctgcc gcttcgactg tatctttgat
      541 gtggcggcga attttgttcg catcttccca agaccacatc gtgaaggcat gctcatcgac
      601 acctgggacg ttgaatgtat tcggctcgcc acccattgcc aagatcaggt agtcatagtt
      661 gaagctatgg ctgctggtcg tcacgacttt cttctcacgg tccacgtgcg tcacttcatc
      721 tgtgacgata tccacattcc ggttacggct gaataggcgt tgcagatcgt attggattgc
      781 atcgggctgt acgcgtcccg ccgccacctc atgtaactcg gtcatatagg tttggtagga
      841 atgtctgtcg atcaaagtga tcaagacatc cggattcttc ttgaaatgtc gggataattt
      901 cttcgcggcg gccacaccgg cgaaacctgc tcccacaacc acaatattct ttttactcat
      961 ttggcagtct cctttttatc tatgttcagc gcatccctgc gcctaatcac ttatttaaaa
     1021 taaccacatt tataagtata gtggatttgt gaataattgt ccatgcaaaa tctgaaagta
     1081 gtatgaaaaa gttctccagc tagcacaatt tcatcctttt ttgcttgttt cctatctcac
     1141 gcgtcgtctt aaaatctttc gtaatcgaaa tatcaaaaca ataaaatgct agtcttgctc
     1201 tttccgataa accaatgaag cctcatacca tgctttcgtt aatttgcttt tagcggcctc
     1261 ctccgactgg aactgacgga ttgtcgagac gtgctccagc aaatagttcg ccgccacccc
     1321 gacagcgacc cctgagaaga taccggttat ggataggatc ggcaagtaga gcatgacgct
     1381 gaacgattac gcgatcagac tcgccatcgc caactgcccg acattgtgca gaatcccacc
     1441 ggtcgcactg atgccgatca tgctgacccg cttcggaccc aaaagtcgga ccaaaagcat
     1501 gccaccataa ctcaaaaaag cccccgcaaa actgtacaag aaggtcgata acgtcccacc
     1561 caataatgtc gaaatcaaca aacgcatcca cacaaccttg aaactatctt tgtacggtag
     1621 cgtaaaaatc gccagtaatg taatgatgtt cgccaacccc agcttcgcac ccggcgcaaa
     1681 cgcgaacgga aacggaatcg cccgctccag catcgtgatg accacaccct gcgcagccaa
     1741 taatgaaata tagatcaaac gtttgttttt attcatgagt atgtccttac ttattaaaat
     1801 tcgtagtttt agtgtagcat aattgagcca aggaaatgaa ctacggacct atatttacta
     1861 aaagaaaaaa acaggaatgc ctcacaaaga gccagccccg ttcttcctct cctccggcga
     1921 acggaagctt cgccgcagtt cgtccaacat ttcctcttcg ttctccgata ggctcggcct
     1981 ctccggagtt gaggttgaaa gttacctctg tcctccgacg agcgaccgct gcccggagct
     2041 acgcattcat ccgagcgctg tcctccgtct agcagcccgg tcctttcttc gctccactgc
     2101 tgcttgccac gaagaaaaga ccgcctccaa agggagacgg ccccgcatta tttggatatc
     2161 attccaacaa acgcttcatt tttcggtggc atctatcata agcgttcttg atggatgtca
     2221 cctcgcagtc caattcatcc gcgatgctca ggaaatccga tccattgaga aaacggatga
     2281 acacagcttg ttcgaaatca gaaagacttt cgaagtaacc cgatgcactt tccttcacca
     2341 taacgatttc ctcaggagag aagctcatcg acacaacacc ttcaaacgaa tgttccgtat
     2401 ggttactgta ctgattttcc aggatcgcat ccaaagatac cgcgcctttg tcgatccttc
     2461 tcttgactgc actctctttc ctgatcaagc tgaaaatgtg atgtttgaga gttagtttat
     2521 aaaaactcgc aaactgcaag cctttctcgg gaacataaga ctgaacagcc ttgtgcaata
     2581 ccatccgagc ctcttgccag aagtcatctt gttccaaaga cttcaagtag taggctctgt
     2641 tgaacttctt gactagtggt aaaaacctat agaatagttc ttcaaagtgc atagagtgcc
     2701 cttgctggat tagtgccacc aattgttccg gactgtattc cgatagttcc acttcttcgt
     2761 tcattttcaa tcccccaagc taatttttgg caaatatata atacatctct agaaaatgcg
     2821 ttacatattg cctaatacct attataccga taaagaaagc gctgtcaaac attagtttga
     2881 atttttattc attaaacttt ccctatatct aaattctcgg tgcagcatta gacgctcata
     2941 gtgataatcg ttacttgcca gctcttgaag gcaattgagc tgtaaccgcg tgtagatcgc
     3001 tccaatacat gcagcgatgt aagaataaat catcttcgcc tcgagattca gcttccaatc
     3061 acttgtcacc gttccccttt tataggttag acctcatcgc tgctggggtc tcttcttaca
     3121 gagatgagga gatcacatgc aacttttgga gcctgattac acttattgat ggtagaggag
     3181 gtaatatgag aacatgcgct atccaatgca tttctgaaca aatccgtgga aatgaagtac
     3241 aaaatccttt tgtcctcgga agtcttcata aagactgaag acagctggaa ttagcttccg
     3301 aaacctatat acggatagta attaatttgg atacgcacag aatatgaccg gctcagatgg
     3361 actgccaatg ctagttatta gtgcgaaagt gttacactat gtggaggaga tgcgcaaaag
     3421 accaattttt actttcaaga atacagttac aatatgctcc cgctattttt tggaaaaata
     3481 gcggcgcttt taccatataa ggaggaacgc atgaaataca caacattaaa tgataatgcg
     3541 attatgccaa cccttgggat cgggacttat cggatccgtc ccacagatgc agaacgttcc
     3601 gtggaacatg ctttgaaaaa cggatatcaa ttgattgata cggcaaatat ttatctgaat
     3661 gaaaaagctg ttggacgagc catcaaaaaa tccggcatcg atcgaagtga gatttttctg
     3721 acctcaaaaa tctggccgac agaatataaa tatgcaaaag cgaagcaagc cattgaggtt
     3781 accctaaagc ggttgggcgt cgattacatt gatctcatgc tgttgcatca accaacaggc
     3841 gcttatctag gcgcttggag agctatggaa gaggcagtaa aaacgggcaa gattaaatca
     3901 attggtctgt ctaactttac aggacttgaa ttggataagg tgctggctga aggaacgatc
     3961 aaacctgctg ttgtgcaggt ggaatgccac ccttatttcc aacaaaaaga tttgaaagca
     4021 aaacttgcta aggacaatat tgcgcttgaa gcctggtacc cccttggctc agctgacaaa
     4081 gacttgcttg ccgaacccat ctttgcagaa ttggcaagta agtacaataa atctgttgtg
     4141 caaatcatct tgcattggca tgtccaaaca ggaaatattg tcattccagg ctcgaaaaat
     4201 ccaactcaca tagacagtaa tctggctatt tttgattttg aactatcatc agctgacttg
     4261 gagcaaatcg cccaactcga caaagacaaa cgcttcttaa atctgccaaa atggatgcac
     4321 cgtctgtttt tgctggcacg cttgaactac gataagcaag tctaagcggc tgcgacaaac
     4381 ggaacgatgc gcacaaagat gtatgaacgt taccgaaaga gcgtcgtaaa gccccttnnn
     4441 nnnnnnnaac agcccttgga acctcctgga tttatccatg gggagcagtc agcattgcaa
     4501 aaagaggaat cccctacctt cctgttgccg taacagaaat cttcacgaat ctccttgact
     4561 tgaagtccag tttaacctgt atactggttt tatatgcgga aaagctaatc ggataatccg
     4621 ggcacagcct tttgtaaccg caatcaataa ccatttttaa aggagaataa tatgaaaaaa
     4681 ccaaacctta tgttactggt cttcactttg atgagctttg tgctggccat gacggctttt
     4741 gtcttcagcg gcattttgga caaagtggct gtttcgctgg acatctcggt ggcccagtcc
     4801 ggtctgttga acacgatgta ttcttatggg gctgcatttg gagtgccgat tactttgatc
     4861 ctgttcagga aggtcgaacg cagcaagatg ct
//
LOCUS       DAOMUJ010000086         4884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4836_length_4884_cov_1.816939, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000086 DAOMUJ010000000
VERSION     DAOMUJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4836_length_4884_cov_1.816939"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /gene="mutS"
                     /locus_tag="PLT73_03345"
     CDS             complement(<1..1778)
                     /gene="mutS"
                     /locus_tag="PLT73_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM19449.1"
                     /translation="MPQKTKNTPMMEQYLSIKKQYPDAFLFYRLGDFYELFHDDAMKA
                     AKLLEITLTSRNKNADEPIPMCGVPYHAAAEYIRRLVEMGHKVAVCEQMEDAKLTKGM
                     VRREVVRVITPGTFLNENGSENKANNYLASVFRNEAGGYALGYVDIGTGELKVTLLTN
                     EDGVFNELQTLPFKEIVLDAKTAEVLTEKLEKQFGILVSIQEKLIPETDVHDLVGELP
                     QQSEKDVLVLLLSYLSDTQKRSLSHLQKAVAYQTDAFLKMDTYARRNLELSASIRTQQ
                     KKGSLLWTLDETKTAMGGRMLKQWLEKPLINLSDILERQRKVESLVNHYFERMDINDA
                     LTSVYDLERLVARVSLGNINGRDLIQLKTSLQQIPGLVQAIATIDEPDVWQDVIGKLE
                     SYPEIIELIERAITDNPPIQITEGNIIRDGYHEQLDRYRDAMNNGKLWIAMLQKEERE
                     KTGIKTLKIGYNRIFGYYIEVTKANLAALPEGTYERKQTLANAERFVTPALKEKESLI
                     LEAEEKSVELEHELFVALREEIKGYSRQLQALAKLVAEIDVLQSFAHVSEAYHFSKPE
                     LSNSDRNLSIKEGRHPVVERVIGKDK"
     gene            complement(1812..2186)
                     /locus_tag="PLT73_03350"
     CDS             complement(1812..2186)
                     /locus_tag="PLT73_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRM19450.1"
                     /translation="MTQELPIEGPTEVELNSLIEILKKNEVITRYQEVKENIKENRAL
                     EQLVEAIKSKQKEAVAFEHYAKPAAAEKTAAELADLNKQLKDSIAVQQYREALWDANE
                     LLENVIGIIQRGIDDAVEKDEV"
     gene            complement(2183..2989)
                     /locus_tag="PLT73_03355"
     CDS             complement(2183..2989)
                     /locus_tag="PLT73_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRM19451.1"
                     /translation="MKVLFIGDVVGSIGRQMLQDTLPQLKKHYRPQVTIVNGENAAGG
                     RGITEKIYKEFLQTGVDVITMGNHTWDNRDIFEFIGTANKMIRPANFPEGTPGIGWTI
                     IKVNQLKLAVVNLHGRVFMNSLDDPFRKADDILEQVRKETNCIFVDFHAETTSEKQAM
                     GWYLDGRVSAVVGTHTHVQTNDARILPDGTGYLTDAGMTGAYDSILGVEKEIIIQKFL
                     TQLPIRHEVPEKGRKLLSGCYVEINDQTGKCVKIENIVINEDRPFGGEFY"
     gene            complement(3132..4688)
                     /gene="rny"
                     /locus_tag="PLT73_03360"
     CDS             complement(3132..4688)
                     /gene="rny"
                     /locus_tag="PLT73_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRM19452.1"
                     /translation="MDIWTLAFAIIALIVGVVVGYLYRKSNHEKEIAGAKNTASQILE
                     DARKEAETTKKEAMLEAKDENHKYRSEIEDELRERRGEVQKQENRLIQREENLDRKDA
                     SLSKREQSIESKEDNLVKRLNALVSEENRIQALVEEQQHELERIATLSREEARHIIMD
                     ETENQLSHEIAVMIRESDQKAKDEADRNAKNIILQAIQRSAADLVSEATVSVVTLPND
                     DMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIAKMALEKLIQDG
                     RIHPARIEEAVEKARKDMDERIREIGEQATFEVGIHSLHPDLIKILGRLHFRTSYGQN
                     VLNHSIEVAKLCGVMAGELGEDINLAKRAGLLHDIGKALDHEIEGSHVEIGSEIAQKY
                     KENPVVINAIASHHGDVEANSIISVLVASADALSAARPGARSESLENYIRRLEKLEGI
                     ANSFEGVASSFAIQAGREIRVMVKPDQLDDSQAIRVAREIRKIEEELDYPGHIKVTVI
                     RETRVIEYAK"
BASE COUNT         1121 a         1226 c          985 g         1552 t
ORIGIN      
        1 aatttgtctt tgccgatgac gcgttccaca accggatggc gcccttcttt gatggacaga
       61 ttgcggtcgc tgttgctcaa ctcgggcttg gaaaaatgat aggcttcgct gacatgagcg
      121 aacgactgca ggacatcgat ctccgccacc aatttggcaa gcgcctgcaa ctgtcggctg
      181 tatcctttta tttcctcccg caaggcgaca aacaactcat gctccaattc gacagacttt
      241 tcctccgcct ccaaaataag cgactctttc tctttcaatg caggtgtgac gaatcgttcg
      301 gcattggcga gcgtctgttt gcgctcatac gtaccttctg gcaacgccgc aaggttcgcc
      361 ttcgttactt ctatgtaata gccaaaaatg cggttgtagc caattttcag cgttttgatg
      421 ccggtctttt ctctttcctc tttctgcagc atcgctatcc aaagtttccc attgttcatc
      481 gcatcgcggt aacgatccaa ttgctcatgg tagccatcgc ggataatgtt gccttcggtg
      541 atttggatag gtggattatc cgtgattgcc cgttcaatca gttcgatgat ttctgggtaa
      601 gattccaatt tgccgatgac atcctgccaa acatccggtt catcgattgt tgcgatggct
      661 tggaccaacc ctggaatctg ctgcagagag gttttcagct ggatcagatc acgaccgttg
      721 atattaccta gcgatactct ggcgaccaaa cgttccagat catacaccga tgtcaaagca
      781 tcgttgatgt ccatccgctc aaaatagtgg ttgaccagcg actccacttt ccgttgcctt
      841 tcgagtatgt cgctcaggtt gatcagcggc ttttcaagcc attgtttcag catccgcccg
      901 cccatagccg tttttgtttc atccagcgtc cacagcaggc tgcctttctt ctgctgcgtc
      961 cggatggatg cggacaactc gaggttgcgc cgcgcatagg tatccatctt cagaaaagcg
     1021 tcggtttgat aagcaacggc cttttgaaga tgtgacaggc tgcgcttttg ggtatccgaa
     1081 agatagctca gcaacaaaac tagcacatct ttttccgact gttgcggcaa ttccccaacc
     1141 agatcgtgga catccgtttc cggaatcagc ttctcctgga tagaaaccag gattccaaac
     1201 tgtttttcca acttttccgt caggacttca gctgtttttg cgtccaggac aatctctttg
     1261 aacggcaacg tctggagctc gttgaatacg ccatcttcgt tggtcaggag ggtgactttc
     1321 agctcccccg tcccgatatc gacatatccg agcgcatacc cgccggcttc atttcgaaag
     1381 acacttgcca agtaattatt tgccttgttc tcgctgccat tctcattcaa aaatgtaccc
     1441 ggggtaataa cccggacgac ttcccgcctc accatgcctt ttgtcagctt ggcatcctcc
     1501 atctgttcgc aaacggcgac cttgtgcccc atttccacga gtctccggat gtattccgcc
     1561 gctgcgtgat agggaacccc gcacatcgga atcggctcat cagcattttt gttgcggctc
     1621 gtcagcgtta tttccagtaa ttttgcggcc ttcatggcat catcgtggaa caattcgtag
     1681 aaatcgccca aacgatagaa taaaaacgca tccggatact gtttttttat ggatagatat
     1741 tgttccatca tcggcgtatt tttagtcttc tgtggcattt tttcacctct atcaatatat
     1801 ctcttcaatc ctcatacttc atctttttcg acagcgtcat cgatgccgcg ttggatgatg
     1861 ccgatgacat tctccaatag ttcgttggca tcccataaag cttctcggta ttgttggact
     1921 gctatgctgt ccttcaattg cttgttgagg tcggctaatt cagcggctgt tttttcagcc
     1981 gctgctggct tagcataatg ttcaaaagcc acggcttctt tttgtttgct tttgatggcc
     2041 tcaaccaatt gttccagcgc ccgattctct tttatatttt ctttgacctc ttggtaacgg
     2101 gtgattactt catttttttt cagtatctcg atcagactat ttaattcaac ttctgtcgga
     2161 ccttcgatag gtagttcttg ggtcaataga attcccctcc aaatggacga tcttcattga
     2221 tcacgatgtt ttcgattttt acgcatttgc cggtctggtc attgatttca acgtagcagc
     2281 cggagagcag ttttcggcct ttttcaggta cttcgtggcg gatcggcaat tgcgtcagaa
     2341 atttctggat gatgatttct ttttcgacac ccaaaatgct gtcataagcc ccggtcattc
     2401 cagcgtctgt cagatagcct gtcccgtcag gcaaaatgcg cgcatcattt gtctgcacat
     2461 gcgtgtgtgt gccgaccaca gccgatacgc gaccgtccaa ataccagccc atcgcttgtt
     2521 tttcgctggt tgtttcggca tgaaaatcga caaagatgca attcgtttct ttgcggacct
     2581 gctcaaggat gtcatccgct tttcggaaag gatcatccag actattcatg aagacgcgtc
     2641 cgtgcaggtt caccacagct aatttcaatt gattcacttt tatgatcgtc caaccgatgc
     2701 caggagtccc ttccgggaaa ttagccggac ggatcatctt gttggctgtt ccgatgaatt
     2761 cgaatatgtc acggttatcc cacgtatggt tccccatggt gatgacatcg acaccggtct
     2821 gcaaaaattc tttatagatt ttttccgtta tgcctctgcc gccagctgca ttctctccat
     2881 ttacgatggt cacttgcgga cgataatgtt tcttcagttg tggaagtgta tcctgcagca
     2941 tctgtctgcc gattgaacct acgacatctc caataaataa tactttcatc ttcagcctct
     3001 tctctcaatt ctagtctttt tatagtaaca tattttgact gtttcgataa gccgttgcac
     3061 ccttgtccag caaacagtat ggtcataaaa agcaaaaaac gggacaaaaa gctgtcccgt
     3121 ttggtttttc attatttggc atattcaatt actcttgttt cgcgtataac cgtaaccttg
     3181 atgtgtcccg gataatctag ttcttcttcg atttttctaa tctcacgagc tacacgtata
     3241 gcttgagaat cgtcaagttg gtctggtttg accatcacgc ggatttctct gcccgcttgg
     3301 atagcaaaac tgctggcaac gccttcgaaa ctgtttgcaa ttccttccag tttctccaaa
     3361 cggcggatgt agttttccag agattcactt cttgcgcccg gacgagcagc agataaagca
     3421 tcagcagatg caaccaatac tgatatgatt gagttcgctt caacatcgcc atgatgggag
     3481 gcaattgcat tgatcacaac cggattttct ttgtatttct gagcgatttc tgagccgatt
     3541 tcaacgtgtg aaccctctat ttcatgatcg agagcttttc cgatatcatg caataaccct
     3601 gcacgtttgg ccaaattgat gtcttcgcct agttctccag ccattacacc gcaaagtttt
     3661 gcgacttcga tgctgtgatt caggacattt tgcccataac tagtgcggaa atgcagacga
     3721 cccaaaattt taatcagatc aggatgcaaa gaatggatac caacttcaaa agttgcttgt
     3781 tcgccgattt ctcttatgcg ctcatccata tccttacggg ctttctcaac tgcttcctca
     3841 attcttgcag gatggatacg tccatcttgg attaatttct ccaaagccat tttcgcaatt
     3901 tctcttcgga taggatcaaa tccgctcaat acgaccgctt cgggcgtatc gtcgatgatc
     3961 aggtcaatac ctgtcaaggt ctccaaagtc cgaatgtttc ttccttcgcg gccaatgatc
     4021 cggcccttca tatcatcgtt aggcaaagta actactgaaa ccgttgcttc cgaaaccaaa
     4081 tcggccgcac ttcgctgaat tgcctgcagg ataatgtttt tcgcattacg atctgcttca
     4141 tctttcgctt tttggtcaga ttctctgatc atgaccgcaa tttcatgcga cagttgattt
     4201 tctgtttcgt ccatgatgat gtgtcttgct tcctcacggg acaatgtagc gattctctcc
     4261 agttcatgct gttgctcttc caccaatgct tgaatgcgat tttcctcaga aactaaagcg
     4321 tttagtcttt tgacaagatt atcctctttt gactcgatag attgctcacg tttcgataaa
     4381 ctggcatctt ttctatccaa attttcttct ctttggatca atcgattttc ttgtttttga
     4441 acctcaccgc gtctttcacg caattcatct tctatttcag agcggtattt gtggttctca
     4501 tccttcgcct ctaacatagc ttcttttttc gttgtttctg cttctttacg agcgtcttca
     4561 agaatttggg aagcggtatt tttggcacca gctatttctt tttcgtgatt cgattttcga
     4621 tataaatatc cgacaacgac accaacaatt aaagctatga tagcgaaggc taaagtccat
     4681 atatccatag tttcacctcc gtatctattt aattttgttg tcaaaaaaat gaataaaaat
     4741 tgattttaca atgtgcagaa tgaagcgata cacataaatt cattctaaac gtagaatact
     4801 gacatgtcaa cgataaaacg gtaacatcaa caaaatacat gtttttctgt tccaatgttg
     4861 tcctttttcg tcttaaaaaa gaaa
//
LOCUS       DAOMUJ010000087         4856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4873_length_4856_cov_2.743803, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000087 DAOMUJ010000000
VERSION     DAOMUJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4873_length_4856_cov_2.743803"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PLT73_03365"
     CDS             <1..1474
                     /locus_tag="PLT73_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM19453.1"
                     /translation="PVYQSPNDDNGYDISDYEAILDEFGTMEEMEHFIAEAEKRNIKV
                     VMDLVVNHTSDEHAWFVEAKKGKDNPYRDYYVWADPAADGGVPNGLGSAFSGPAWEWD
                     AASGQYYLHLFSKKQPDLNWQNPKMRQEIYAMMNRWIAKGIGGFRMDVIDLIGKIPEK
                     GITGNGPQLHEFLQEMQQATFGNHDLLTVGETWGATPEIAKLYSAPERNELSMVFQFE
                     HIGLDQKSNGEKWDLQPMKVAELKRILAKWQTELGHDGWNSLFWNNHDLPRMLSRWGN
                     DQEYRSESAKMLAILLHMMKGTPYIYQGEEIGMTNTPITDIAEARDIETLNMYNERLA
                     KGYSEADILASINAKGRDNARRPMQWNASSHGGFTTGTPWIAVNKNYPAINAEAALAD
                     PDSIFYTYQKLIRLRKENPIIIWGEFELVDTVDEVISYYREYEGERWLVVANFSDAAQ
                     PFSAEDEIAEVIIQNDGNEITSLKDIQLKPWQAFVVKVSA"
     gene            1471..3099
                     /locus_tag="PLT73_03370"
     CDS             1471..3099
                     /locus_tag="PLT73_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM19454.1"
                     /translation="MSRWWKNAVGYQIYPRSFRDANHDGIGDLQGIIEKLPYLQSLGV
                     DFIWLNPVYASPNVDNGYDISDYQDIHPDFGTMADFEELLGKAHAAGIKIIMDLVVNH
                     TSDQHPWFLEAKKGKDNPYRDYYLWADATENKLPNAWQSFFGGSTWTYDDNSGQAYFH
                     VFAPEQPDLNWKNPQVREEIYRMIRWWLDLGIDGFRLDAISHIQKEPWDFRITDNPWA
                     PFMNVAGIEVYMQELKEIFDAYPIMTVGEASGVSSKKAVHWTKEDGYLNMIFELEHNV
                     REGQPGAERLNLLGFKKVLSRWQKDLGANGWNALYVENHDNPRIASILGDESPKSAKA
                     IATMYMLLKGTPFIYQGQEIGMTNFAFESMEQVDAQDSHNLYRILLDSGASPEDALRN
                     VTHWTRDHSRTPLQWEGGMFGGFSELLPWMAVNANTAQVNIFDQEKDEQSVLSHYKHL
                     IRLRKAQPIYAEGAFELILKNHPQVFAYLRKSDTKQVLVLTNLSRNPCQIDLPTRIAN
                     KKWQLLSTNTTDVPAVAKRLQMQPYDAWVFEEIG"
     gene            complement(3383..4480)
                     /locus_tag="PLT73_03375"
     CDS             complement(3383..4480)
                     /locus_tag="PLT73_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HRM19455.1"
                     /translation="MTVSYEKFDLKEVINASGRMTILGVSKVSQAVLAAQQYGGEHFF
                     EMEDLSVKVGAYLADLLKAEDAQVVSSASAGIAQSVAGVIGKGSLFHAYHPYAETITQ
                     REIILPKGHNVDYGTPVEVMVQQGGGKVVEAGYANVCTPEHVEMMITKETAALLYVKS
                     HHTVQKSMLTVDEMVAVARKYDLPVIVDAAAEEDLFGYYAAGADLVIYSGAKAIEGPS
                     AGLVVGKKSAIEWVRLQGKGIGRAMKIGKDNILGFAQAVEDYLANGSESGESMKARLV
                     PFIEAVNQIPALQATVVQDSAGREIYRGSVKVSGEIDAKEVIRQLKTQNPAIYTREYQ
                     ANNGIIEFDIRSVNQTEMMKIVQRLREILGE"
BASE COUNT         1198 a         1312 c         1247 g         1099 t
ORIGIN      
        1 gcctgtctac cagtcgccga acgacgacaa cgggtatgat atttccgact acgaagccat
       61 tctcgacgag ttcggcacga tggaagagat ggagcatttt atcgcggaag ccgaaaagcg
      121 caacatcaaa gtggtgatgg atctggtggt caaccatact tcggatgagc acgcctggtt
      181 tgtcgaagca aaaaaaggca aagacaatcc gtaccgcgac tattacgtct gggctgatcc
      241 tgccgcagac ggcggcgtgc cgaacggatt gggctccgcc ttctccggtc cggcttggga
      301 atgggatgcg gcgagcggtc aatattactt gcatcttttc agcaaaaaac agccggacct
      361 gaactggcag aatccaaaaa tgcgccagga aatctacgcg atgatgaatc gctggatcgc
      421 taaaggcatc ggcggcttcc gcatggatgt catcgacctg atcggaaaaa tccccgagaa
      481 aggcatcacc ggcaacggac cgcagctgca tgaattcctg caggaaatgc agcaagcgac
      541 tttcggcaac cacgacctgc tgaccgtcgg cgaaacctgg ggagcgaccc cggaaatcgc
      601 caaactgtac tctgctcctg agcgcaacga actgtcgatg gtcttccaat tcgaacatat
      661 cggcctggac caaaaatcga acggcgagaa atgggatctg cagccgatga aagtcgccga
      721 actgaaacgg attctggcga aatggcagac cgaactcggg cacgacggct ggaattccct
      781 gttctggaac aaccacgatt tgccgcggat gctttcgcgc tggggaaatg accaggaata
      841 ccgttcggag agcgcgaaaa tgctagcgat cctgttgcac atgatgaaag gcacgcccta
      901 catctatcaa ggcgaagaga tcggcatgac caatacgccg atcactgata ttgccgaagc
      961 aagggacatc gaaacgctca atatgtacaa cgagcgcttg gcgaaaggct attccgaagc
     1021 agatatcctg gcttccatca atgccaaagg ccgcgacaat gcccgccgcc cgatgcagtg
     1081 gaacgcttct tcccacggcg gatttacgac aggcacccct tggattgccg tgaacaaaaa
     1141 ttatccggcc atcaatgcgg aagctgcgtt ggccgatccg gattcgatct tttatacgta
     1201 ccaaaaactg atccgcttgc gcaaagaaaa cccaattata atttggggcg aattcgagct
     1261 ggtcgatacg gtcgacgaag tcatcagcta ttaccgggaa tacgaaggcg aacgctggct
     1321 agtcgtggcc aacttctctg atgccgcgca accgttctcg gctgaagacg agatagccga
     1381 agtgatcatc cagaatgacg ggaatgagat taccagtttg aaggacatcc aactgaagcc
     1441 ttggcaagct ttcgtggtga aggtgagcgc atgagcagat ggtggaaaaa tgcagtaggc
     1501 tatcagattt atccccggag tttccgggac gccaaccacg acggcatcgg cgacttgcag
     1561 ggaatcatcg aaaaactacc ctatctgcaa tcattgggcg tggatttcat ctggctgaac
     1621 cctgtttacg cttcgccgaa tgtcgacaac ggctatgaca tttccgatta tcaggacatc
     1681 catcccgatt tcgggacgat ggccgatttc gaggaactgt tgggcaaagc ccatgctgca
     1741 ggcatcaaaa tcatcatgga tctggtggtg aaccatacga gcgaccagca tccttggttt
     1801 ctggaagcaa aaaaaggcaa agacaatcct taccgcgact attatttgtg ggcggatgcc
     1861 acggaaaaca agctgccgaa cgcttggcaa agcttcttcg gcggctcgac ctggacttac
     1921 gatgacaact cgggccaggc ctatttccat gtctttgcgc cggaacagcc cgatctgaac
     1981 tggaaaaatc cgcaagtgcg ggaagaaatc taccggatga tccgctggtg gctggacttg
     2041 gggatcgacg gcttccgttt ggatgcaatc agccatatcc agaaagaacc ctgggatttc
     2101 agaatcaccg ataatccttg ggctcctttc atgaacgtcg cggggatcga agtctatatg
     2161 caggagctga aggaaatttt tgatgcctac ccgatcatga cagtcggcga agcaagcggc
     2221 gtttccagca aaaaagcggt acattggact aaagaggacg gctatttgaa catgatcttc
     2281 gagctggagc ataacgtgcg cgaaggccag ccgggcgcgg aacggctcaa tttgttgggg
     2341 ttcaaaaagg tgctttcgcg ttggcaaaag gatttggggg cgaacggctg gaacgccctg
     2401 tacgtggaga atcatgacaa tccgcggatc gcttcgattc tgggggacga atcgcccaaa
     2461 tctgccaaag cgatcgcgac gatgtacatg ctgctgaaag ggacgccctt catctatcaa
     2521 ggtcaggaaa tcggcatgac caatttcgcg ttcgaatcga tggagcaagt cgacgcccag
     2581 gattcgcaca atctttaccg gattctcttg gacagcggtg cctccccgga agatgccttg
     2641 cgcaacgtca cccactggac gcgggaccat tcgcgcaccc cgctgcagtg ggaaggcggc
     2701 atgttcggcg gattttccga gttgctgcca tggatggccg tcaatgcgaa caccgcgcag
     2761 gtgaacattt ttgatcagga aaaggacgag cagtccgttt tgagccacta caaacacctg
     2821 atccggttgc ggaaggcaca gcccatctat gccgaagggg cgttcgaact gattctgaaa
     2881 aatcatccgc aggtctttgc ttatctgcgg aaatcggaca caaaacaggt gctcgtgctc
     2941 acgaacctga gccgcaaccc ttgccaaatc gatctgccga cacggattgc caacaaaaaa
     3001 tggcagttgc tgtcgacgaa tacaacggac gtgcctgctg ttgcaaagcg gctgcagatg
     3061 cagccctacg atgcttgggt gtttgaggaa atcggctgat tgcctgctta tcggagttgc
     3121 gcgacggatt gggtgccctg ctccggtgag ggttcccaca ccggcggaca gcggtaattt
     3181 gcaggcgctt ctcctgagag cctgtcctag tcggagcaca gtcccctgaa tgtgggccga
     3241 cctccggcaa agcctccgtt cgccggagga ggacaatcga gtgccacaaa aaacaaacag
     3301 cgaatggaac gcctacaata ggttgtccat tcgctgtttg tttttgtctt tctggtaata
     3361 aacggtggga atcacttttt catcattctc ccaaaatttc ccgcaaccgc tgcacaatct
     3421 tcatcatttc cgtttggttg acggaacgga tatcaaactc gatgatgccg ttattggctt
     3481 gatattcgcg cgtatagatc gccggatttt gcgtttttaa ctgacggatg acttctttgg
     3541 catctatttc tccagacacc ttgacactgc cccgataaat ctccctgccg gcactgtcct
     3601 gcacgacagt ggcttgcaaa gcgggaattt gattgaccgc ttcgataaag ggcactagac
     3661 gcgccttcat cgattcgccg ctttcgctgc cattagccag ataatcttcc acggcttggg
     3721 caaaccccag aatattgtct ttgccgatct tcatggctcg gccaatcccc ttaccttgca
     3781 gccgcaccca ttcaatcgcg ctttttttgc cgacgaccaa gccggcgctc gggccctcga
     3841 ttgctttggc tcctgaatag atcaccaagt ccgcccccgc tgcgtaatag ccgaataaat
     3901 cttcttccgc tgcggcgtcg acgatcaccg gcaagtcgta ctttcgggcc actgccacca
     3961 tttcgtccac cgtcagcatg cttttctgca cggtatggtg gcttttgaca taaagcaggg
     4021 ccgccgtttc cttggtgatc atcatttcca catgctccgg agtacacaca tttgcatatc
     4081 cggcttctac gactttgccg cccccttgct gaaccatgac ttcaaccggc gtgccgtaat
     4141 ccacattatg gcctttgggc aaaatgattt cccgctgcgt gattgtttct gcataaggat
     4201 ggtaggcatg aaacaaactg cctttgccga tgactcctgc cacggattga gcgatgcccg
     4261 ccgaagcaga agacacaact tgggcgtctt ccgctttgag caaatcagcc aaataagcgc
     4321 cgactttcac cgacaaatct tccatctcaa aaaagtgttc tcccccatat tgttgggcag
     4381 ccagaacagc ttgcgatact ttcgatacac ccaaaatcgt catccgaccg gaagcattga
     4441 taacttcctt caaatcaaac ttctcataac tgaccgtcat agcattgtcc tccaataatg
     4501 atagggattc ttggatattc gttgcagcta tcggatttcc ttcaccgacc ctaaaagtag
     4561 gctgtcctat tctgattccc tggataatgt tgtataacat catcatacat cagccattcg
     4621 ttgctgtaca atcattttca aaatttcgtt tgttggagtt gcccatttga atcgatgccc
     4681 tcctcctgtg aggctcccct cgccggagtt cagcggtatc ttacgggttc tcctcctgcg
     4741 agtccgtcct cttcggagca caactcaaaa tgcgggccgt gctccggcaa agcctccgtt
     4801 caccggagga gaacaacgaa aggccgcctc gaaatgagac agcctgcatt cgttcc
//
LOCUS       DAOMUJ010000088         4840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4896_length_4840_cov_2.278370, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000088 DAOMUJ010000000
VERSION     DAOMUJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4896_length_4840_cov_2.278370"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>399
                     /locus_tag="PLT73_03380"
     CDS             <1..>399
                     /locus_tag="PLT73_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM19456.1"
                     /translation="LFGHFLEQRTLNQTRSAIKELTDMAPESALRQLANGEFVEVDVD
                     DVDEGDILLVKTGAKVPVDGTILTGEGHINEASITGESVPASKTIDSAVFAGTILENG
                     TIQIRADRVGEDTTFGRIIELVEEAQDSKS"
     assembly_gap    400..409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <410..1626
                     /locus_tag="PLT73_03385"
     CDS             <410..1626
                     /locus_tag="PLT73_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM19457.1"
                     /translation="FIDKFSKYYTPAVLVLAFVVWMISQNVELAITVLVLGCPGALVI
                     GVPVSNVAGIGNGARNGVLLKGSEVIHDFSRVDTIVFDKTGTLTIGNPEVAEKEIYAE
                     NVNEVLGYLASVERESDHPLAKAVLTNIGTTTFSTVEETEVVKGGGIVAKVNGHRIAV
                     GNVALMEKENVKLSKKAQADVKRFEQNGNSLVLTAVDGNLQVLMGIRDQIRPGVKNDL
                     EGLRKLGVKNLVVLSGDNQGTVDLVARELGLTDAHGHMLPEDKSAHIEKLQKEGQIVA
                     FVGDGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSDFSRLPHALGLTKATAANM
                     KQNIIIALGVVVVLVASLFFSDWMNMSIGMLVHEGSILVVIFNAMRLLKFRLKGNRNN
                     VKTGVKEIVTAK"
     gene            1755..2024
                     /locus_tag="PLT73_03390"
     CDS             1755..2024
                     /locus_tag="PLT73_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRM19458.1"
                     /translation="MDAVLIPAKKGLFKDDIFEIADSLLLDKDDLVQKGYGWMLKSLC
                     TKYEDEVYQYVLSKRDTMPRTALRYAIEKMPKPMKQEAMKREKKK"
     assembly_gap    2213..2222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2572..2919
                     /locus_tag="PLT73_03395"
     CDS             2572..2919
                     /locus_tag="PLT73_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HRM19459.1"
                     /translation="MQVGELLTAKGESNYKSGLKMNHIYFTAVISGAGLAAALAKGDG
                     SERIYIVEPTGDFENDPNVTDKKFPGNLTRSYRSQAPLKIVGEATEWLRQTPEDLRRW
                     QEKLADNKGEIIN"
     gene            3066..4778
                     /gene="ilvD"
                     /locus_tag="PLT73_03400"
     CDS             3066..4778
                     /gene="ilvD"
                     /locus_tag="PLT73_03400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM19460.1"
                     /translation="MENEKNKKDLRINSFVYDGVVKSPNRAMLRATGMHDEDFKKPMV
                     GVISTWAENTPCNIHLNDLAAIAKDGIKKQRGWPIQFTTVTVSDGIAMGTPGMNYSLP
                     SRDLIADSIEVAVGGQNLDAFISIGGCDKNMPGSVIAMANANIPSIFVYGGTIAPGQD
                     EDGNDIDLVSVFEAIGKWNNGEMTEEEVRAIECNACPGPGACGGMYTANTMASAIETM
                     GLSLPGSSSNPAATGDKLKDVFEAGEAIIGLIEKNIRPRDIITRKALENAVVITMALG
                     GSTNAILHLLAIAHAAEVDFAIEDFNEIQKRVPHLADLKPSGKYVFQDLYNVGGVPAV
                     QKYLLDNGLLHGDCLTVTGKTLAENLENVAPLAEGQDVIMSLENPKRKDGPLIVLKGN
                     LAPDGAVVKVSGLKQRRHEGPAKVFNTEEAAIEATLNGEIVDGDVVVVRFVGPKGGPG
                     MPEMLSLSSIISGKGIKVCLITDGRFSGGSHGFVVGHIAPEAQDGGPIALLETDDTVI
                     IDQDTRELTMKVSDEELAKRREKLVLPPLKSRGVLGKYAHIVSSASKGAVTDFFNRNP
                     FEEK"
BASE COUNT         1410 a         1018 c         1262 g         1130 t
ORIGIN      
        1 cttgttcggc catttcttgg aacaacgcac cttgaatcaa acccgttccg ccatcaaaga
       61 gttgaccgac atggcgccgg aaagtgccct cagacaactg gcaaacggcg aatttgtaga
      121 agttgacgta gatgacgtgg acgaaggaga tatcctgttg gttaaaaccg gtgcgaaagt
      181 tcctgtagac ggcaccatcc tcactggtga aggacacatc aatgaagcca gtattaccgg
      241 agaatctgtc ccagcaagca aaacaatcga ttcagcagtt tttgccggta ccattttgga
      301 aaatggaacc atccaaatcc gcgcagaccg ggtcggggaa gacaccactt tcggccgcat
      361 cattgaactc gtagaagaag cacaagattc caaatcggan nnnnnnnnng cttcatcgat
      421 aagttttcca aatactacac gccagctgtt ttagtcttgg cctttgttgt ctggatgatc
      481 agtcaaaacg ttgaactggc catcacagtg ttggtcctag gatgtccagg tgccttggtg
      541 atcggcgttc cggtttcaaa cgttgccggt attggtaacg gagcgcgcaa tggcgtccta
      601 ctaaaaggta gcgaggtcat ccatgacttc agccgcgtag acaccattgt ctttgataaa
      661 acgggaacct taaccattgg taatccagaa gttgctgaaa aagaaattta tgcagaaaac
      721 gtgaacgagg tgcttggcta cttggcaagt gtggaacgcg aatcggacca ccctttggct
      781 aaagcagttc taacgaatat cggaacgacc actttttcca ccgttgaaga aaccgaagtg
      841 gttaaaggcg gtgggattgt agccaaagta aacggccacc gcatcgcagt cggaaacgta
      901 gccttgatgg aaaaagaaaa tgtcaaacta agcaaaaaag cacaagcaga tgtcaaacgc
      961 tttgaacaaa acggcaactc actagttctg accgctgttg acggtaacct gcaagtgttg
     1021 atgggcatcc gcgaccaaat ccgtccaggc gttaaaaatg acctggaagg tttgagaaaa
     1081 ctaggcgtga aaaacttggt cgtcctttcc ggtgacaacc aaggaacggt tgacttggtc
     1141 gcccgtgaat taggactaac tgatgcacac ggccatatgt tgccggaaga caaatcagcc
     1201 catatcgaaa aattacaaaa agaaggtcaa atcgtggcct ttgtcggtga cggtgtcaac
     1261 gacagtcctt ctctagcttt agcagatatc gggattgcga tgggtagcgg aacggacgtc
     1321 gccatcgaaa catctgatgt ggtcttgatg aattctgact tcagccgctt gccacacgca
     1381 ttaggcttga cgaaagcaac tgctgcaaac atgaaacaaa acatcatcat tgcgttaggt
     1441 gttgtagtgg tactggtagc cagtctcttc ttcagtgatt ggatgaacat gtcaatcggg
     1501 atgttggtac acgaaggtag catcttggtc gttatcttca acgccatgag attgttgaag
     1561 ttccgcttga aaggcaacag aaataatgtg aaaacgggcg tgaaagaaat tgtaacagcg
     1621 aaataagaat aaatgacaaa gaaaaatcag gatgaacaag tatactgaaa tgcaccaata
     1681 cgcgaactaa ccaatcacta caccatcttt tttagaaccg gaaagaatcc ctattctttc
     1741 tggttctttt tttattggat gctgtattaa taccagccaa aaaaggtctg ttcaaagacg
     1801 atatatttga aattgccgac agcttattgt tggataagga tgatttagtg caaaaagggt
     1861 atggctggat gctgaagtct ttatgcacca aatatgaaga tgaggtatac cagtatgtat
     1921 taagcaagcg ggacacaatg cctagaactg cattaaggta tgctattgaa aaaatgccaa
     1981 agccaatgaa gcaagaggcc atgaaaagag agaagaaaaa gtgagtaaga gtcagcattc
     2041 tcactttcaa agcttttgta atctacattt atgtgctgca aaaagcaggg gctttttgag
     2101 gagcagagta tcattatagt tcagttggcg agtcaataat taagaggtga aatcagatgg
     2161 ataatcaatt caatccgaac aacaagctca ttcaactgct tcttcagggg atnnnnnnnn
     2221 nngatgggca tggaagacag cggcaaacct gaagaggcaa gcctgctgtt ttctaaggcc
     2281 tggagtgaag cgacagccga ttttgaaaaa ttcattgcag cttattatgt ggcccggcat
     2341 caaaaaaatg tatcagacaa attaaaatgg ttcgaaacct ctttgcagtt tgcgctaaaa
     2401 gtgaatgacg aggcggttaa gagcgctttc ccatcgttgt atttgaacat cgcgaaatgc
     2461 tatgaggcgt tatccgatcc ggacaaggca aaaaagtatg ttgcattatc gcattcgtat
     2521 aagggtacgc cttctgatcc aggtcctttt tttcatggga caagagcaga tctgcaggtc
     2581 ggcgagctgc tgacagcaaa aggagaatcg aattacaaat ccggacttaa aatgaaccac
     2641 atttatttca cggctgtgat cagtggggcg ggacttgcgg ccgcattggc gaaaggtgat
     2701 ggatcggaac ggatttatat tgtggagccg acaggggatt tcgaaaatga cccaaatgtt
     2761 acagacaaga aatttccagg gaatctgacc cgttcctacc gttcgcaagc acctttaaag
     2821 attgtcggcg aagcaacgga gtggttgcga cagacgcctg aggaccttcg tcggtggcaa
     2881 gaaaaattgg ccgataataa aggcgaaatc attaattgag tacgcaactt tcgccgcgta
     2941 aatatggagc gttttccgcc atgtcctgat ttagggaggg cccctgatag ttttttgtgg
     3001 gcagaaatgt taaaatctga ttagtagtaa gtgtttgtgc atgaaaatac gggagggatt
     3061 ttggaatgga aaacgaaaag aacaaaaaag atttacgcat caacagcttc gtttatgatg
     3121 gagtcgtgaa atcgccgaac cgtgcgatgt tgcgtgcgac aggcatgcat gacgaagact
     3181 ttaaaaagcc gatggtaggg gtaatcagca cttgggctga gaacacgcct tgcaatatac
     3241 atttgaacga cttggctgca atcgcaaaag acggcatcaa gaagcaacgc ggttggccga
     3301 tccagtttac gacagtgacc gtttcagatg gtatcgccat gggtacgccg gggatgaact
     3361 attcattgcc ttcgcgcgat ctgattgccg attcgatcga agttgcggtg ggcggccaga
     3421 atctggacgc attcatcagt atcggtggct gtgacaagaa tatgccgggc tctgtcatcg
     3481 cgatggcaaa tgcgaatatt ccatccattt ttgtttatgg gggtacaatc gcaccggggc
     3541 aagatgaaga tggcaacgac atcgatctcg tatccgtttt tgaagccatc ggaaagtgga
     3601 acaacggtga aatgacggaa gaagaagttc gggcaatcga atgcaacgcc tgtcctggac
     3661 caggggcttg cggagggatg tatacagcca acacgatggc atcggcaatc gaaacgatgg
     3721 ggctgagttt gccgggatca tcgtcaaatc ctgcagcaac cggtgataaa ttgaaggatg
     3781 ttttcgaagc aggcgaagcc atcatcggct tgatcgaaaa aaatatccga ccgcgcgaca
     3841 tcatcacgcg caaagctctg gaaaatgccg tagtgatcac gatggcattg ggcggatcga
     3901 cgaacgccat tttgcatctg ttggcgatcg cccatgccgc tgaggtggac tttgcaatcg
     3961 aagacttcaa tgaaatccaa aaacgggtac cgcatttggc tgacctgaaa ccttcgggaa
     4021 aatatgtctt ccaagatcta tacaatgtcg gcggggtgcc tgccgttcaa aaatacctgc
     4081 ttgacaacgg tttgctacac ggcgattgtt tgaccgtaac cggaaagaca ctggcggaaa
     4141 atcttgaaaa tgtggcacct ttggcagaag gccaggacgt gatcatgtcg cttgagaatc
     4201 cgaaacgcaa agatggtccg ctgattgtcc tgaaaggcaa tcttgccccg gatggcgctg
     4261 ttgtaaaagt ttccggactg aagcaacgac gccatgaagg acctgcgaag gtgttcaaca
     4321 ccgaagaagc agccatcgaa gcgaccctga acggggaaat cgtggacggc gatgtggtgg
     4381 ttgtccgctt tgtcgggccg aagggtggac cgggaatgcc ggaaatgctc tccttatcaa
     4441 gcatcatttc aggaaaaggc atcaaagtat gtctgattac cgatggtcgt ttttccggcg
     4501 gttcccacgg tttcgtggtg ggacatatcg caccggaggc acaagacggt ggaccgatcg
     4561 cgttattgga aacggatgat acggtcatca tcgatcagga tacgcgtgag ttaacgatga
     4621 aagtatctga tgaagaattg gctaaacgac gcgaaaaact ggtgttgccg cctttgaagt
     4681 cgcgcggtgt cctgggcaaa tacgcacaca tcgtatcctc tgcatcgaaa ggtgccgtaa
     4741 ccgacttctt caaccgcaac ccatttgagg aaaaataaag gagggtgccg catagtggaa
     4801 aagggcatca cgtacactgg tctggctggc cagcacaagg
//
LOCUS       DAOMUJ010000089         4837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_4901_length_4837_cov_2.248013, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000089 DAOMUJ010000000
VERSION     DAOMUJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4901_length_4837_cov_2.248013"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLT73_03405"
     CDS             complement(<1..387)
                     /locus_tag="PLT73_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19461.1"
                     /translation="MSRYDGPSFFRHDHHSGMDGSHPATNKFDFPNYLKKEDEEHGML
                     EEKGDSAQPSSYISQDLLHPMDTVPYIRHNKEAFVSFRDKVVKPAEDVFAVEKKRLKE
                     EKQKERRTELGMHPFASYKSRRPFQLT"
     gene            complement(489..1103)
                     /locus_tag="PLT73_03410"
     CDS             complement(489..1103)
                     /locus_tag="PLT73_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HRM19462.1"
                     /translation="MWLSFYNREAVGDTLMLTKGDIAVRQEQACESKGNVTRIFNNRT
                     NETMGYNLFSISDTLALDANGQVLLSDEEAEKVNALIQAAGFSDAIVVDRSPKFVVGY
                     VKECVPHEDSDHLSVTQTEVDNGEVLQIVCGAANIKKGQRVVVAKPGAVMPSGLIIWP
                     GELRGVASQGMICSAKELQLENASPKKGILVLDDSYAIGAAFFK"
     gene            complement(1157..1468)
                     /locus_tag="PLT73_03415"
     CDS             complement(1157..1468)
                     /locus_tag="PLT73_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM19463.1"
                     /translation="MDKLKDLDQFKELRDSGKTVFVFMTGWCPDCHYIRPFMPEVENK
                     FTDFRFVEIDRDDFGTLGEALQISGIPSFIVYAEGELLGRFVSRNRKTQKEIEAFLEQ
                     L"
     gene            complement(1483..2559)
                     /gene="pepA"
                     /locus_tag="PLT73_03420"
     CDS             complement(1483..2559)
                     /gene="pepA"
                     /locus_tag="PLT73_03420"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HRM19464.1"
                     /translation="MDDKTFAMIKELTELQGTSGNERNIRNYMRERMAPLVDRIETDG
                     LGGIFGIREHEDETAPRIMVAAHMDEVGFMVSGITERGLFRVVPLGGWNAYVVSAQRY
                     TLQTSKGDIPVISSSVPPHLLRGEGEGKKVKVTDILFDAGFDTKEEAMAFGVRPGDTI
                     VPQVETIWTANKKKIISKAWDNRYGNTVVLETLEALKNEKLPNTLIAGSNVQEEVGLR
                     GTKGAVHKFKPDLFFAVDCSAADDLTTTKDTFGHLGEGFLLRIQDPGMITLKGMREFL
                     LDTAETHDIPYQYFVSKGGTDAGAAHVMNDGVPSAVIGVCARYIHTHQTMFHIDDYAA
                     AKEMVAQVIKALDKSTYETIMAMN"
     gene            2814..3164
                     /locus_tag="PLT73_03425"
     CDS             2814..3164
                     /locus_tag="PLT73_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM19465.1"
                     /translation="MNFLEKCMCKKHKEEILGGILFGAGLVLGSVVTALCYEQKRTVN
                     GDKILENVKKMFLAEAPIEGSWIELHPVPLSRYSSKTDVYYGGISRKEDGVLVQYEFI
                     ADAYTGTILDLYKL"
     gene            complement(3227..3892)
                     /gene="trmB"
                     /locus_tag="PLT73_03430"
     CDS             complement(3227..3892)
                     /gene="trmB"
                     /locus_tag="PLT73_03430"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRM19466.1"
                     /translation="MRVRNKPWAAEKLAESTQYVVMEPADWKGKWQERFGNSQPIHVE
                     IGSGKGQFIVEMARMHPEVNYIGIERQTSVAVMTLDKMIESGLKNVQLLNTDGENVAD
                     FFEAGEVDRVYLNFSDPWPKSKHDKRRLTYKKFLQNYEQILVSGGEIHFKTDNQGLFE
                     YSLASFSQYGMTLKQVWLDLHKSDFEGNIMTEYEAKFSSRGDRIYRVEAAFPKKATAD
                     SEN"
     gene            complement(3965..4762)
                     /locus_tag="PLT73_03435"
     CDS             complement(3965..4762)
                     /locus_tag="PLT73_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRM19467.1"
                     /translation="MDYKMDSGWRLHPIGGDTGQAYMGTRAEERVFLKRNSSPFLAAL
                     SVEGITPRLIWTKRVGNGDVLTAQEWLNGRELNPDEMRGQSVRDILHRIHHSENLLKM
                     LQKVKGEIFNPINFYNLYVADLPDDLKSNSVLQQAAEYVKNSITSIDDSQKTVCHGDV
                     NRKNFLLDEEDRLYLVDWEMVKIADPFSDISTLLVQYVPHEEWRVWLEQYGLQMNDNM
                     ERRLEWYSMMVCLCLIKKSHSQERNVEMNQLILLLKQLYQNRLNSYL"
BASE COUNT         1143 a         1254 c         1039 g         1401 t
ORIGIN      
        1 ggtcagttgg aaaggtcttc tgctcttata ggatgcgaaa ggatgcatgc ccaattccgt
       61 gcggcgctcc ttctgctttt cttcctttag tctttttttc tcgactgcaa aaacatcttc
      121 ggcaggcttc acaactttgt ccctgaagga aacgaatgcc tctttgttgt ggcggatgta
      181 agggacagta tccatcggat gcagcaaatc ttgtgagata taggaggacg gttgtgcaga
      241 atcgcctttt tcttccagca tgccatgctc ttcatcctct tttttcaggt aattcggaaa
      301 atcaaactta tttgttgcag gatgacttcc atccatccct gaatgatggt catgccgaaa
      361 aaaactgggt ccatcatagc gactcatcca gtcaactcct ctcttgtttt gcttcaggca
      421 atgcgctgcc tggtttcaat ggaattcctt tattatagta cttctttttc cgacaaaatt
      481 ccatagggtt atttgaaaaa cgctgcgcct attgcatagc tgtcatccaa cacaaggatg
      541 ccttttttag ggcttgcgtt ctccaattgc aattctttgg cggaacagat catgccctgg
      601 ctggctacgc cgcgcaattc acctggccag atgatcaaac cactaggcat gacagcgcct
      661 ggtttggcca cgacgacgcg ttggcctttt ttgatgttgg ctgcaccgca gacgatctgc
      721 agcacttcac cgttgtccac ttccgtttgc gtaacggaca ggtggtcgga gtcttcatgc
      781 ggcacgcatt ccttgacgta acccacaaca aatttaggcg aacgatcgac gacgatggcg
      841 tcactgaaac ctgctgcttg gatcaaagca ttcacttttt ccgcttcttc atcggataac
      901 agcacttgtc cgtttgcatc caacgccagc gtgtctgaaa tcgaaaatag attgtagccc
      961 atcgtttcat ttgtacggtt gttgaaaatg cgcgtcacat tgccttttga ttcgcatgct
     1021 tgttcttggc ggacagcgat atcccctttc gtcaacatta atgtatcccc gactgcttca
     1081 cgattataaa aacttaacca catgttatgt ttgctccttt atgcttcttc atttattttt
     1141 cccaaccgaa atagtatcac aattgttcca aaaaggcttc aatctctttt tgcgtcttcc
     1201 ggttgcgtga gacgaaacga cccaatagtt ctccctcagc atagacgatg aagctcggga
     1261 ttccggagat ctgtaacgct tcacccagcg tgccgaaatc atcgcggtct atctcgacga
     1321 aacgaaaatc cgtaaattta ttttccacct caggcatgaa tgggcggatg taatggcaat
     1381 ccgggcacca accggtcatg aagacgaaga ccgtttttcc cgaatcacgc agttctttga
     1441 attgatccag atctttcagt ttgtccataa tcaagttcct ccttagttca tggccatgat
     1501 cgtttcataa gtgctcttgt ccaatgcttt gatgacttgc gcaaccattt ccttggctgc
     1561 cgcgtaatcg tcgatgtgga acattgtctg atgtgtgtga atatagcggg cgcagacgcc
     1621 gatgaccgcg ctaggcaccc cgtcattcat gacatgggcg gcacccgcat ccgttccgcc
     1681 tttggaaaca aaatactgat aagggatatc atgcgtttcc gctgtatcca acagaaattc
     1741 gcgcatccct ttcaaggtga tcatgcctgg atcctggatc cgcaaaagga aaccttcccc
     1801 cagatggccg aacgtatcct tggtcgtcgt caaatcatcc gcagccgagc aatccaccgc
     1861 gaagaacaga tccggcttga acttgtgcac ggcccctttt gttccacgca gtccgacctc
     1921 ttcttgcacg ttggagccgg ctatcaaagt gttcggcaac ttttcatttt tcaatgcttc
     1981 cagcgtctcc aaaacgaccg tattcccgta gcggttgtcc cacgcttttg aaatgatttt
     2041 cttcttgttg gcggtccaaa ttgtttccac ctggggaaca atcgtatctc cgggacggac
     2101 tccgaatgcc atcgcctctt cttttgtatc gaaccccgca tcgaacagga tgtccgtcac
     2161 tttgactttt ttgccttcac cctccccgcg caataagtgc ggcggaaccg aagaggaaat
     2221 cacaggaata tcgcctttgg acgtctgcag ggtatagcgc tgtgccgaaa cgacataagc
     2281 attccaaccg ccgagcggca cgacccggaa caatccgcgc tccgtgatgc cggacaccat
     2341 gaaacccact tcatccatgt gtgccgcgac catgatgcgc ggtgccgttt catcttcatg
     2401 ctctctgatt ccgaaaatcc cgcccaagcc atccgtttcg atgcgatcca ccaaaggcgc
     2461 cattctctcg cgcatatagt tacggatgtt gcgttcgttt ccgcttgttc cctgcaattc
     2521 cgtcaattct ttgatcatag caaatgtttt atcatccata tttgaatgga gcagggcata
     2581 aatttagcct tatgccgctc ccgatccccc ttaagtgtct gattatccta tccattatag
     2641 cgcagcatcc ttgtcatagc aacgattcca tttgttaaga aagcttgccc agtacaggaa
     2701 acgtttcctt tgcgcatcct ggtgcttctg acatttccct attatttttt tcgtattatt
     2761 gctatactga aggagaaaat gagtaagcgc attactttga ggaggaacat aagatgaact
     2821 ttttggaaaa atgcatgtgt aaaaaacaca aagaagaaat cttgggcggt attctgttcg
     2881 gtgccggact cgtactcggt tcagtcgtga cagccctttg ctatgagcag aaacggaccg
     2941 tcaacggcga taaaatcctg gaaaacgtca aaaagatgtt cttggctgaa gctccgatcg
     3001 aaggatcttg gatcgaactg caccccgtcc cgctcagtcg ttattcttca aagacggatg
     3061 tgtattacgg tggtatatcc cgcaaagagg acggcgtgtt ggtgcaatat gaattcattg
     3121 ctgacgccta cacgggaact atcttagatc tttataaatt gtaaaaaaac agaaaaagga
     3181 aggaacaaag ctgcgagtgc ttcgttcctt cctttttgtc tgggttctaa ttttcagaat
     3241 cagccgttgc ttttttcggg aacgctgctt cgacgcggta aatgcggtct cctcgggaag
     3301 agaacttcgc ttcgtactcc gtcatgatgt ttccctcaaa gtcgctcttg tgcagatcca
     3361 accacacttg cttcaaagtc attccatatt gcgagaagct cgccaacgaa tattcgaata
     3421 agccttggtt atccgtcttg aaatggatct caccaccgga aaccaggatc tgttcatagt
     3481 tctgcaggaa ttttttgtag gtcaagcggc gtttgtcatg tttggacttt ggccacggat
     3541 cagaaaagtt caggtaaacg cgatccactt cgcccgcctc aaagaaatct gctacattct
     3601 ctccatccgt attcagcaat tggacatttt tcagacctga ttcaatcatt ttatcgagtg
     3661 tcatgaccgc cacgctagtc tggcgttcga ttccgatata attcacttcc gggtgcatgc
     3721 gggccatctc gacaatgaac tgtcccttgc ctgagccgat ttccacatgg attggttggc
     3781 tattgccgaa acgttcctgc catttccctt tccaatccgc tggttccatc acaacatatt
     3841 gggtactttc agccaatttt tcggcagccc atggtttatt tcttacgcgc ataattctcc
     3901 tctttcttat ctgttttttt caaaaaaaga aatcctttcc gttcgtatga tgacgaagag
     3961 gatttcataa gtaactattc aagcgattct gatagagctg tttcagcaac agaatcagtt
     4021 gattcatttc cacattgcgt tcctgggagt ggctcttttt gatcaggcag aggcacacca
     4081 tcatgctgta ccactccaaa cggcgttcca tattgtcatt catctgcaag ccatactgct
     4141 ccaaccatac tctccattct tcatggggaa cgtattgtac cagcaaggta ctgatgtcag
     4201 agaacggatc ggcaattttg accatttccc aatccaccag atacaaccgg tcttcctcat
     4261 ccagcaggaa atttttccga ttcacgtctc cgtggcagac cgttttttgc gagtcgtcga
     4321 tgctggtgat gctgttcttc acatattcag cagcttgttg cagtactgaa ttggatttaa
     4381 ggtcatccgg cagatccgcc acatacaggt tgtagaaatt gatcgggttg aaaatttcgc
     4441 cttttacctt ctgaagcatc ttcaggaggt tttcggaatg atggatgcga tgcaggatat
     4501 cccgaacgct ctgccctctc atttcatccg gattcaactc cctgccgttg agccattctt
     4561 gagctgtcag gacgtcccca ttgccgaccc gtttggtcca gatcaaccgc ggcgtgatcc
     4621 cctcaacaga aagcgcggct aagaaaggtg aagagtttct tttcaaaaag actctttcct
     4681 cggctctggt gcccatataa gcttgtcctg tgtctcctcc gatgggatgt agccgccacc
     4741 ctgagtccat tttataatcc atcgagctca ttccttactc tgtcattaat tttgaaaagc
     4801 ggtacaagcc cgctctgcct cgaaagaaat ttaggaa
//
LOCUS       DAOMUJ010000090         4739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5032_length_4739_cov_2.214347, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000090 DAOMUJ010000000
VERSION     DAOMUJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_4739_cov_2.214347"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..784)
                     /locus_tag="PLT73_03440"
     CDS             complement(158..784)
                     /locus_tag="PLT73_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 family protein"
                     /protein_id="HRM19468.1"
                     /translation="MAGYHFDKADFNVFDIQGLDERMAGIRDRIQPKFRHFAEAGAHL
                     IVSEAGPESVPVHVAKHLRRTKYAPENTWCAIGGDARGYKKYPHFQIGIAKEGVSFYL
                     CLIDQPVKEKEMAAALLERIPELERLPHDYVVSVDHTVPDVLPIEAVNWESILIRLRD
                     VKKAELLIGRKLAPTDPRLATEAGTLAVLEDTFRELLPIYQACMEHYR"
     gene            complement(854..1801)
                     /locus_tag="PLT73_03445"
     CDS             complement(854..1801)
                     /locus_tag="PLT73_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19469.1"
                     /translation="MKKWQKTIISLASIFTLAACGAQEGAADAADSTTVTSNAETETT
                     VTIEDARGSVEVPKDPENVAVLDFGHLDTLIALGKQDAVTGTATDNMPAYLADKADQF
                     ENVGTLKEPNVEALANLAPELIIISNRLLDFAEQLEEIAPVVVLSVDYTDYWGSVQKN
                     ITTLGVIFDEEEAAEEAIVTLNEEIESVKAKTAGISEKTLTLLLNDGSMSAFSTGSRF
                     GFIYVDAAIEDSTHGQSVGYEGLLEINPQILFVIDRTAALGTASDENAALLENDFVYQ
                     TDAYKNNKIINLSSDLWYLSGGGIESIHLMIEEIAVGYQ"
     gene            complement(1820..2581)
                     /locus_tag="PLT73_03450"
     CDS             complement(1820..2581)
                     /locus_tag="PLT73_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19470.1"
                     /translation="MKISHVSKAYEDEKVLDDISLAIERGKITAFIGPNGAGKSTLLS
                     VISRLLTKDEGILTIHGKEIEAWQSDELARELSILKQQNAYQVRMTVRELVSFGRFPY
                     TKGRLNEEDHAMIDKVLGYLNLVEFSNRYLDTLSGGQLQRAAIAMILVQDTEYILLDE
                     PLNNLDLKQSIVTMQTIRNLADELGKTILVVIHDVNFASVFSDNIIAMKNGKVFRSGT
                     VEEVIRTQVLQELYEVPLEVITTADGKKHCTYQAI"
     gene            complement(2578..>2927)
                     /locus_tag="PLT73_03455"
     CDS             complement(2578..>2927)
                     /locus_tag="PLT73_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM19471.1"
                     /translation="MGLGTQTAKNLGISVEKETKLTFMHVILLMTVSAALVGPMMFLG
                     FVAANIAYRLFRTYCLNYLLPGASLLSFVMVVAGQFLAEEVFKLQTNLSILVELFGGI
                     YFFWLLWKERSKL"
     assembly_gap    2928..2937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2938..3339)
                     /locus_tag="PLT73_03460"
     CDS             complement(<2938..3339)
                     /locus_tag="PLT73_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM19472.1"
                     /translation="MTPSILGVESFYRLLQTVLMFFSFSLLGSQLNAEWQFVFLVFLM
                     LGSYFLLYRLSWNRKNYDMQIILMIGLVMGTFFNSISSFMQVLMDPNEYDKLQTKLYA
                     SFQNINDSVLVLAVPIAMFSIISLWRKRKVLE"
     assembly_gap    3349..3358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3359..3553)
                     /locus_tag="PLT73_03465"
     CDS             complement(<3359..3553)
                     /locus_tag="PLT73_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19473.1"
                     /translation="MKNVLKNRIIMKRLAVMAVAVALVYFFWNQGIGNLFIMKLRSTK
                     LLTFAVISISTAFATVSFQTI"
     gene            complement(3550..4521)
                     /locus_tag="PLT73_03470"
     CDS             complement(3550..4521)
                     /locus_tag="PLT73_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM19474.1"
                     /translation="MEDGEEMLKKLLLTLALAGVIVLSLSVGTAAFSVEQFLVGSNTD
                     RLILLSSRLPRTVTVLLAGAGLSLSGLIMQSLTQNRFAAPDTVGTVDAARVGMLCGMI
                     LFPGLSVVGKMALSFFFAAAGTFLFLVVVNRLPFKSQMTIPLLGLMYGNILGGVANFF
                     AFRFNVTQNLSAWMQGDFSLVIKGQYEGMYLIFLLFLALYFFADHFTIARFGKDAAKN
                     LGLPFEAVLFIGTLFVSLTVAVILSTVGNLPFLGVIIPNLVSLRFGDNIRNTHGKVAL
                     FGAVFLLLCDIFSRAVIPPYEIPVETVLGVFGGGLFLWLLLKGGRTA"
BASE COUNT         1316 a         1240 c          989 g         1174 t
ORIGIN      
        1 tcgcagagac aagaggttcc ttattctttt ttccgaaggg ctaacccgtg aagctagcca
       61 acgttttgga tgctcaaaat atttttcaag ccaaaaagga ctgaacagca atcaatctcg
      121 ctgttcagtc ctttttagtt tgtattgggt tacacgttta gcggtagtgt tccatgcagg
      181 cctggtagat cggcagcagt tcacggaaag tatcctccaa cactgccagc gtacctgctt
      241 ccgtcgctaa gcgcggatcg gttggcgcca acttgcggcc gatcaacagc tcagctttct
      301 tcacatcccg caaccggatc aggatagact cccaattgac tgcctcaatc ggaagcacat
      361 ccggcacggt atgatcgacc gaaacgacgt aatcatgcgg caatctttcc agttccggaa
      421 tccgttccaa taaagcggcc gccatttctt tctctttgac gggttggtcg atcagacaaa
      481 ggtagaagct gaccccttcc ttcgcaatcc cgatttggaa gtgaggatat ttcttgtagc
      541 ccctcgcatc cccaccgatg gcgcaccagg tattctcggg cgcatatttt gtgcggcgca
      601 gatgcttggc gacatgtaca ggcaccgact cgggacccgc ttccgaaacg atgaggtggg
      661 cacccgcttc cgcaaaatgc ctgaacttgg gctggatccg gtcgcggatg cctgccatcc
      721 tttcatcgag tccctgaata tcaaaaacgt tgaagtctgc cttatcgaaa tgataacctg
      781 ccaaattgtg cacctctttc tgatttgctt ccatcattat agagaaaaaa tcagaagctg
      841 gacaattttt tgcttactgg tagcccactg cgatctcttc gatcatcaaa tggattgatt
      901 ctatcccacc acctgaaaga taccacaagt cggaagacag gttgatgatt ttattgtttt
      961 tgtaagcatc cgtttgataa acgaaatcat tctccaacaa cgccgcattt tcatcagagg
     1021 ctgtcccaag agctgccgta cggtcgatga caaacaaaat ctgcggattg atttccaaca
     1081 acccttcgta cccgacggac tgcccatgtg tggaatcctc aatcgctgca tcgacataga
     1141 tgaagccgaa acgcgagcct gtgctgaaag cggacatgct gccgtcattc aggagtaacg
     1201 tcaatgtttt ctcgctgatt cctgctgtct tcgctttgac ggactcaatc tcctcattca
     1261 aagttacaat ggcttcttca gcggcttcct cttcgtcaaa gatgactcct aaagttgtga
     1321 tgtttttttg cacagatccc caatagtccg tgtaatcgac gctgagcacg actacagggg
     1381 cgatttcctc caactgttcc gcgaagtcca acaagcggtt ggaaatgatg atgagctccg
     1441 gcgccaaatt cgccaatgct tccacattcg gttccttaag tgtccctaca ttctcgaatt
     1501 gatctgcctt atccgccaaa taagccggca tgttgtctgt cgcagttcct gtgactgcat
     1561 cttgcttgcc caaggcgatc aatgtgtcca aatggccaaa atccaaaaca gctacatttt
     1621 caggatcttt cggtacttct acggagccac gcgcatcctc gatggtcact gtcgtttcag
     1681 tttccgcatt cgaagtcact gttgttgagt ctgccgcatc ggcagcccct tcttgggcgc
     1741 cgcaagccgc caaggtaaag attgaagcca aactgatgat tgtcttttgc cattttttca
     1801 ttttccattc tcctctatgt taaattgctt gatacgtgca atgttttttt ccatctgcag
     1861 tcgtgatgac ttccaatggc acttcatata attcctgcag aacttgagtc cggatcactt
     1921 cctcgaccgt gccgctccgg aagactttcc catttttcat ggcgatgatg ttgtccgaaa
     1981 aaacggaggc gaaattgacg tcgtggatga cgaccagaat ggttttaccc aattcatccg
     2041 ccaaattccg gatggtctgc atcgtcacaa tgctctgctt caaatcgaga ttgttcagcg
     2101 gttcatccaa taagatatac tcggtatcct gcaccaagat catcgcgatg gccgcccgct
     2161 ggagttgccc cccagacaac gtatccaaat agcggttgga gaattccacc aggttcagat
     2221 agcccaaaac cttgtcgatc atagcatgat cttcttcatt cagccttccc ttcgtatagg
     2281 ggaacctgcc gaacgagact agttcacgga ccgtcatacg cacttgataa gcattctgtt
     2341 gtttcaggat cgaaagctcc ctcgccagtt catcggattg ccaggcctca atttctttcc
     2401 cgtgtatcgt caatatgcct tcgtcctttg tcaacagacg gctgatgact gaaaggagcg
     2461 tacttttgcc tgccccgttt ggaccgatga aagccgttat tttacctctc tcgatagcca
     2521 aagagatatc atccaatact ttttcatcct cataggcttt gctgacatga ctgattttca
     2581 taacttactc ctttccttcc ataataacca aaagaaatag attcccccga acagttcgac
     2641 cagaatgctt aaattcgtct gcaacttgaa aacttcttct gccagaaatt gtccggccac
     2701 gaccatcaca aaactgagta gactcgcacc aggcagcaga tagttcagac aatacgtcct
     2761 gaacagacga taagcgatat tcgccgccac aaaaccgaga aacatcatcg gcccgacgag
     2821 cgctgcagag acagtcatca acaggatgac atgcatgaag gtgagcttgg tctccttttc
     2881 gacggagatc cctaaatttt tggctgtttg tgtacccagt cccataannn nnnnnnnttc
     2941 caacaccttc cgcttgcgcc acagactgat gatcgaaaac atggcgatcg gaacagccaa
     3001 aacaagtaca gaatcattga tgttctgaaa actggcatac agcttcgtct gcagtttgtc
     3061 gtactcattc ggatccatca gcacctgcat gaaggaactg atactgttga aaaaagtccc
     3121 catgacaagc ccgatcatca aaatgatttg catatcgtaa tttttccggt tccacgaaag
     3181 ccggtacagc aagaaataac tgcccaacat caagaatacc aggaaaacga actgccattc
     3241 cgcattcaat tggctgccca gcaacgagaa gctgaagaac atcaacactg tctgcagaag
     3301 gcggtagaag gattcgactc ctagaatgct aggggtcaaa aagttactnn nnnnnnnnaa
     3361 tcgtctgaaa gctgactgtc gcaaaagcag tgctgatact gatgaccgca aatgtcaaaa
     3421 gtttcgtgct ccgcaacttc ataatgaaca aattaccgat accctggttc caaaaaaaat
     3481 aaaccaaagc cacagccact gccatcaccg ccagccgctt cataatgatg cgatttttca
     3541 acacgttttt catgcagtcc gccctccttt cagcaacagc cacaagaaca ggccaccgcc
     3601 gaaaacaccc agtaccgttt ctaccggtat ttcgtatgga gggataaccg cgcgcgaaaa
     3661 gatatcgcac agcaacagga aaaccgctcc aaaaagcgca accttgccgt gggtattgcg
     3721 gatgttgtcg ccgaagcgca atgatacaag attcgggatg atgactccca aaaacggcag
     3781 atttccgacc gtactcaaga tgacagcaac cgtcaaggat acgaatagtg tcccgatgaa
     3841 caggaccgct tcaaaaggca aaccaagatt tttggccgcg tctttcccaa aacgcgcaat
     3901 cgtaaaatgg tcggcaaaaa aataaagtgc caaaaataac aggaaaatga gatacattcc
     3961 ctcgtattgt cctttgatga ccaatgagaa atctccttgc atccaggccg aaagattttg
     4021 cgtgacgttg aagcggaaag caaagaaatt agctacgcct cccaaaatat ttccgtacat
     4081 caaccctaat aaagggattg tcatctggct cttgaaaggt aagcgattca ccaccaccaa
     4141 aaaaaggaag gttcccgctg ccgcaaagaa gaaagagagg gccatctttc ccacaaccga
     4201 taaacccgga aagagaatca tgccgcagag catcccaact ctcgctgcat ccaccgttcc
     4261 gactgtatca ggggcagcaa aacggttctg tgtcaaggat tgcatgatca acccggataa
     4321 agacaatccc gcacctgcca agagaaccgt aaccgtcctc ggcagccttg acgacaatag
     4381 gatcagacga tccgtgttac tcccgaccag aaactgttca accgaaaacg cagccgttcc
     4441 cacactcagg gaaagcacga ttactcccgc caatgcgaga gtaagcagta atttcttcaa
     4501 catctcttca ccatcttcca aaaacaactc cttaatgaga atgattctaa ttgagaatca
     4561 ttctcaatta atggtataga tgttattcta atggatgagg aaataaaaca caataccaaa
     4621 tatgagattt tcatgaaaaa aagtaagtta ttttaaggaa agcgtcgcca atatccagaa
     4681 aaatagtaaa gaccgaaaaa gtaagggcgc gtcatcaatg actggctttt actttttcg
//
LOCUS       DAOMUJ010000091         4723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5056_length_4723_cov_1.854970, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000091 DAOMUJ010000000
VERSION     DAOMUJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5056_length_4723_cov_1.854970"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLT73_03475"
     CDS             complement(<1..329)
                     /locus_tag="PLT73_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRM19475.1"
                     /translation="MILDEIIAATKIRVEKAKQAIPLEEMKQAALAMPINQDFPFEKA
                     LTGEGIRFICECKKASPSKGVIVEDFPYLEIAKAYEEAGASALSVLTAPDFFQGENRF
                     LTEIKQAV"
     gene            complement(326..1393)
                     /gene="trpD"
                     /locus_tag="PLT73_03480"
     CDS             complement(326..1393)
                     /gene="trpD"
                     /locus_tag="PLT73_03480"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010619307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRM19476.1"
                     /translation="MLEIKETKQTEEILPQDSMKAAIDKLVKKEELSPQLTKTIMHQI
                     MSGQASPVQIASYLTAMRMKGETAGEISASAEVMRDFSNKVQTKTTALDIVGTGGDQA
                     FTFNISTVSSFVIAAAGIPVAKHGNRSSSSKCGSADVLEELGVNIEASAQRSEAMLEE
                     IGMCFMYAQKYHPSMKHAAPVRKEMGVRTIFNVLGPLANPANANMQLLGVYDVALVDT
                     MAEVLNQLQMERALVFGGNDGLDEITLTTTSEVAELRDGKVTHFTFDPKDYGFEYCTN
                     EDLVGGEPAENAAIALAILSGEKGPKRDTVLLNAGMAIYLANDDYTIADGIKRAAELI
                     DSGAALGKLKQFITASQEATK"
     misc_binding    complement(1444..1686)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1848..2366
                     /locus_tag="PLT73_03485"
     CDS             1848..2366
                     /locus_tag="PLT73_03485"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRM19477.1"
                     /translation="MFRLKQSDKLYSMAPVIDPDAKVLILGSMPGAKSLEAQHYYGNP
                     RNHFWEIIFSLLEKEDPVTYEDRLSLLKKHHIALWDVIHSCYREGSLDSAIKEEQPND
                     LKTLLREHPQIKAIAFNGGKAYRSYKKHFGLDEHSGLVYLPLPSTSPMRGKHVKSLPE
                     KLEAWSVLNEYL"
     gene            complement(2513..>2585)
                     /locus_tag="PLT73_03490"
     CDS             complement(2513..>2585)
                     /locus_tag="PLT73_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003642732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM19478.1"
                     /translation="RLSIGIENADDIIADLDQAFEQI"
     assembly_gap    2586..2618
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2619..>2974)
                     /locus_tag="PLT73_03495"
     CDS             complement(<2619..>2974)
                     /locus_tag="PLT73_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003642732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM19479.1"
                     /translation="TGACIGPIDSFLLLQGIETLSLRVERHVENTRKVVAYLANHPKV
                     SWVNYPELPDSKYKALSDKYFPKGAGSIFTFGIKGGKQAGIEWIDKLELFSLLANVDD
                     AKSLVIHPASTTHAQL"
     assembly_gap    2975..2995
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2996..3795)
                     /locus_tag="PLT73_03500"
     CDS             complement(<2996..3795)
                     /locus_tag="PLT73_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003642732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM19480.1"
                     /translation="MTEKNYSFETLQVHAGQVPDPVTGARAVPIYQTTAFVFDSAEQA
                     AGRFALTDAGNVYTRLTNPTTAVVDARVAALEGGTSAVTVASGSAAITYAILNVAHAG
                     DEIVAASTLYGGTYNLFSATLPNLGIKTTFIDPEDTANFEAAITEKTKAIFIESIGNP
                     STNLIDIEKVAEIAHKHGIILIVDNTFGTPYLIRPFEFGADVVVHSATKFLGGHGTTM
                     GGVIVESGKFDFAASGRYPGFTTPDAHYNGLVYTDLGPGAFTTKIRVQ"
     misc_binding    complement(3949..4192)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4314..4646
                     /locus_tag="PLT73_03505"
     CDS             4314..4646
                     /locus_tag="PLT73_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HRM19481.1"
                     /translation="MKLTFTENAIQRLEKLGMIEDLSLHMSTIYGCGGPDSSMFTIRA
                     YDAENLNYDTFLDSNFGKIKATEDSLQQLDEDNIIDFNDSSFSFVLKSNRGLLNPHMN
                     YENMKLKD"
BASE COUNT         1096 a         1213 c         1093 g         1267 t
ORIGIN      
        1 actgcctgtt ttatctccgt caggaagcga ttttcgcctt ggaaaaaatc gggcgccgtc
       61 aataccgata gcgcggatgc gcctgcttct tcgtaggcct tggcgatttc gaggtatggg
      121 aaatcctcca caatcacgcc tttcgaaggg gacgccttct tgcattcgca gatgaaacgg
      181 atcccttcac cggtcagcgc cttttcgaat gggaaatcct gattgatcgg catcgccaag
      241 gcggcttgct tcatttcttc caatgggatt gcctgtttgg ctttttccac gcgtattttt
      301 gtcgctgcta tgatctcgtc caatatcatt ttgtcgcctc ctgtgatgca gtgatgaatt
      361 gctttaattt ccccagtgcc gctccgctgt cgatgagttc ggctgcacgt ttgatgccgt
      421 cagcgatcgt ataatcgtcg ttggccagat aaatggccat gccggcgttc aacagcactg
      481 tatcgcgctt cggtcctttt tcgccactta ggatagccaa ggcgattgcg gcattttcag
      541 ccggttcccc gccaaccaga tcttcgttgg tgcagtactc gaagccgtaa tctttcggat
      601 cgaatgtgaa gtgggtgacc ttgccgtcgc ggagctccgc tacttccgat gttgtggtca
      661 aagttatttc atccagaccg tcattgccac cgaacaccaa agctcgttcc atctgcaatt
      721 ggttcagcac ttcagccatc gtgtccacca gcgcgacatc atacacgccg agcaactgca
      781 tattcgcatt ggctgggttc gccaatggcc ccaggacgtt gaagattgtc cggacaccca
      841 tttctttcct gacgggtgcc gcgtgtttca ttgaaggatg gtatttttgg gcgtacatga
      901 agcacatccc gatttcttcc agcatcgctt cgcttcgctg tgcgctggct tcgatgttca
      961 cacctaattc ttccagcaca tctgcgctgc cgcatttgct tgaggagctg cggttgccgt
     1021 gtttcgccac cggaatccct gcggctgcga tgacaaaaga agagacggtt gagatattga
     1081 aagtgaaggc ttgatcgccg ccggttccga cgatgtccaa tgccgttgtt ttcgtctgca
     1141 ccttatttga aaaatcacgc atcacttcag cggaagcact gatttctcca gccgtctcgc
     1201 ctttcatccg catagcagtc aggtaagatg caatctggac cggactggct tgaccgctca
     1261 taatctggtg catgatggtc ttcgtcaact gcggggaaag ctcttctttt ttcactagtt
     1321 tatcgatagc ggctttcatg ctgtcttgcg ggagaatttc ttcggtctgt ttcgtctctt
     1381 ttatctctag catttcattc acctcatcat tttttgtgta caaaaaaagc ccttgtcccc
     1441 ctttaaaggg acaagagctt gcattctcct gcgataccac ccaaattgac gataataatc
     1501 gcccactcgt tcacatacca acatatgtgc ctgtctgtta acggatcagg ttccccgttt
     1561 gcccctactc ttttcaaggc aaccctctca agtccattcg gcatccgctt cgctatcgca
     1621 ctctcactgg cggcgactct ctggaaacaa ggtatgatgc gtactcctct tgattatcgg
     1681 ttttattaga tggaaatgat ttttctaacg atttcttaat gttgggatta ttttaacgca
     1741 tcgctatgct tccgtcaact gttttattta tataatttgg caagctcgaa tgtttcctgc
     1801 tccagcacgg ttttggtaga atggatgcac aataaccttg aaagggtgtg ttccgactga
     1861 aacaatccga caaactttac tcgatggcac ccgtcatcga tccggacgcc aaggtcctga
     1921 ttctgggatc gatgcccggc gcgaagtcgc tggaagcaca gcactactac gggaatccca
     1981 ggaaccattt ctgggaaatc attttctccc tgctagagaa agaggatccg gtcacctacg
     2041 aggatcgcct ttcgcttctg aaaaagcatc atatcgccct ttgggatgtc atccactcct
     2101 gttaccgtga aggcagcctc gattcggcca tcaaagagga acagccgaac gacttgaaga
     2161 ccctgctgcg cgaacatccc cagatcaaag ccatcgcctt caacggcgga aaagcttacc
     2221 gcagctacaa aaagcacttc ggactggatg agcactccgg acttgtttac ctgccgctcc
     2281 cctcgaccag ccccatgcgc ggcaagcatg ttaaatcgtt gccggaaaaa ttggaagctt
     2341 ggtcggtgct gaatgagtat ctttgaggca tacggaattc agaagatgtc cgatattact
     2401 gatgaatcgg acaaccgccg tttggtttgg gcactcgttg tccgattaat gtcccccaga
     2461 aaaaagccgg tctgtcagaa cccaaattct gacagaccgg ctttcgcatg ttttagattt
     2521 gttcgaaagc ttgatccaag tcggcgatga tgtcatcggc attttcgatc ccgatggaca
     2581 gacggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct caattgcgcg tgggtcgtgc
     2641 tggccggatg gatgaccagc gatttcgcat cgtcaacatt cgcaagcagc gagaacaatt
     2701 ccaacttgtc gatccactcg atgccggctt gcttgccgcc cttgatgccg aaagtgaaga
     2761 tcgagccggc gcctttaggg aagtatttgt cagaaagcgc cttgtatttg ctatccggca
     2821 gttccggata attcacccag gaaactttcg ggtggttcgc cagataagcg acaaccttgc
     2881 gggtattttc gacatggcgt tcgacgcgca atgacaaagt ttcgattccc tgcagcaaca
     2941 ggaaggaatc gatcgggccg atacaggctc ccgtnnnnnn nnnnnnnnnn nnnnnaattg
     3001 cacacggatt ttggtcgtga acgcgcccgg tcccagatcc gtatagacca atccgttgta
     3061 atgcgcatcc ggtgttgtga agccagggta tcttccgctg gccgcaaaat cgaatttacc
     3121 ggattcgacg atgacaccgc ccatagttgt gccatgccca cccaggaatt tagtggcgga
     3181 gtggaccacc acatcggcac cgaattcgaa tggacggatc aggtaaggcg tgccgaacgt
     3241 gttatccacg atcaagatga tgccatgctt atgcgcaatt tcagcgactt tttcgatatc
     3301 aatcagattg gtgcttgggt ttccgatcga ttcgatgaag atggctttcg tcttttccgt
     3361 gatggctgct tcgaagttag ccgtatcttc aggatcaatg aaagtagttt tgatgcccaa
     3421 atttggcaaa gtcgcgctga aaagattgta ggtgccgccg taaagcgtgc tggcagcgac
     3481 gatctcgtca cccgcatgcg caacattcag gatagcgtac gtgatcgcag cagaacctga
     3541 agcgaccgta accgctgatg ttccgccctc aagcgccgca acgcgtgcat ccacgactgc
     3601 ggtcgtagga ttcgtcaaac gggtatagac attcccggca tccgtcaaag cgaaacggcc
     3661 ggcagcctgt tcggcgctat caaaaacgaa agcggtagtt tggtaaatcg gcactgcacg
     3721 ggctccggta acggggtccg gtacttggcc tgcatgtact tgcaacgttt caaaactgta
     3781 atttttctct gtcatttgtc atctttccct tctggttttg gatatattct tgcatggcag
     3841 ttgctcttct gggtcatttc cgatttgccg aggttgcttc ttctgttcat ttctctgatt
     3901 ttgcaaaaag taaaaaagcc ctcgtcccta tattccaact gattgaaata cagggacgaa
     3961 agctcttcgt gttaccaccc atgttcgtag ggacctcgcg atccatacct taaaaagtac
     4021 gcaagctccc gcttgttata ctcttgtgcg ctatcgtgca caaaccgaaa caacactatc
     4081 cgcattgctt tgctccgaga ccatcttcgc tttttttcat catacccctt ttcacctacc
     4141 ggggctctct ggaatgacga tcgaaaggta ctcttctctt catcgctctt atattaaaat
     4201 ttaattaaat ataacatccg attctcacct tgtcaaacat tttgttgatg tgagcatatc
     4261 caatctgatt cacgttgtca tgctctataa tgaaaggaaa gaggtgattt aaattgaaat
     4321 tgacattcac agaaaatgcc atacaacgac tggaaaaatt gggaatgatc gaggaccttt
     4381 ctttgcatat gagtaccatc tacggctgcg gcggaccgga cagcagcatg ttcacgatcc
     4441 gagcgtatga tgcggaaaac ctgaactatg acacttttct ggactctaac tttggcaaga
     4501 taaaagccac cgaggacagc ctgcaacaac tggatgagga caacatcatc gacttcaacg
     4561 acagcagctt ctctttcgtc ctgaaaagca atcgcggact tctgaacccg cacatgaact
     4621 acgaaaatat gaagttgaaa gattaaaaaa acggcaacag attaagtgcg cattccttct
     4681 ggcagaaatt ctgtcagtgc taaactaaat tgaacagttt tcg
//
LOCUS       DAOMUJ010000092         4720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5060_length_4720_cov_2.227010, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000092 DAOMUJ010000000
VERSION     DAOMUJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_4720_cov_2.227010"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1113)
                     /gene="chrA"
                     /locus_tag="PLT73_03510"
     CDS             complement(22..1113)
                     /gene="chrA"
                     /locus_tag="PLT73_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRM19482.1"
                     /translation="MTYTKEIYKEICFVFARLGLFAFGGPAAHIAMMEDELVTKREWL
                     KKTEFMDLLGFTNLIPGPNSTELAIALGYKRGGKIGLFLAGICFIFPAMTIVLLLAMF
                     YKQYGEVPLIEALFSGIKPVILAVIIQALFRLGQTTIKDSKSLLLFLAAYLLSLMGVG
                     EITILILTGLTMLVTQQVSKWRSHHLAIEPMSLMLLFLTFLKIGSVLYGSGYVLLAFL
                     QAEFIERYAALTSTQLLDAVAIGQFTPGPVFTTATFVGYLIQGVPGAILATVGIFLPS
                     FLLILLLHPLFNRLRSSEIFSVILDGITIASLALMASVSWQLGMATFVSWVSVVIFAA
                     SFIGLTRFKVNSSYLIIMGGFIGWLVTMF"
     gene            complement(1150..1926)
                     /locus_tag="PLT73_03515"
     CDS             complement(1150..1926)
                     /locus_tag="PLT73_03515"
                     /inference="COORDINATES: protein motif:HMM:NF005597.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM19483.1"
                     /translation="MGYYDQHMHTHFSPDSAESFENYLEQTDGLLVTTEHLDFHDAYN
                     GGVDTVLDYATYRDKIDALNTIHDGRIRCGIEVGYTPESHAQITAYLEGKAFDVILLS
                     VHQNGRYDYLQPIIDEMDPKAVMKEYFELCTQAVRQVDGANVFAHFDYGIRRLPVTVD
                     DLREFEPLLKGLLDEIMAKEMALELNTRSMYEYNNADLYRYMIGLYLEMGGTRFSLGS
                     DAHSIQKYRYHFDDAIALLKDLGVHELTLFKNQVGYTEPI"
     gene            2131..3630
                     /locus_tag="PLT73_03520"
     CDS             2131..3630
                     /locus_tag="PLT73_03520"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRM19484.1"
                     /translation="MAARYVMGVDVGTTSTKAVLYETDGSAYESAYAHYPLIKENPEM
                     AEQDLDEIFSAVLKTIKAVIQKAELGPTDIAGIGFSSAMHSLILMDAEGTPLTRSITW
                     ADNRSKKYASMLKHSGMGQRFYEKTGTPLHPMSPISKILWLKAEKPEVFQAARWFIGI
                     KEYILWRLFGEFVVDYSVASATGLFNIREFRWDEEILEFCGITSQMLPLPVNPGKQLS
                     GLSEEVAELLGLAADTPFIVGGNDGCLANLGMGAIRAGTATITIGTSGAVRMTSEKPY
                     VSPEGRTFSYILDESHYVNGGAVNNGGNIFDWAIKQFLPANVADEDLYDKAMEMIANV
                     APGADGLVFHPYLNGERAPLWNADARGNFSGINMLHTKEHFLRSVLEGICLNLKDVLA
                     TVISMNGEPTKIMASGGFSRSLAWRQILTDIIGMPIEFPETFESSCTGAALITLKSLG
                     LVESVDDAEKLMGASIEHQPNEDSKSVYAAMFPRYQQMSRLLQEFYTEQ"
     gene            complement(3696..4178)
                     /locus_tag="PLT73_03525"
     CDS             complement(3696..4178)
                     /locus_tag="PLT73_03525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013727140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRM19485.1"
                     /translation="MSIYEYSATKTDQSSVSLEQYKGKVICIVNTASKCGLVGQLDEL
                     EELYEKYKDKDFIILGFPSNQFNNQEPLNGVEAAEFCRLNYGVTFPIFDKIEVNGENA
                     DPLYKYLVEQTGGGAIKWNFTKFLIGRDGEIIKRFAPITAPKKMTKLIESALEQPNPL
                     "
     gene            4408..>4720
                     /locus_tag="PLT73_03530"
     CDS             4408..>4720
                     /locus_tag="PLT73_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRM19486.1"
                     /translation="MFEGLSQKIVGKKIKIVFPEGLEPRILGAVVRLKAEDLVEPIVI
                     GNVDAVKEAAKGRGFRLNGIQIIDPTDYAELDEMVASFVARRNGKVTEEQAKKLLLDE
                     NY"
BASE COUNT         1309 a         1061 c         1180 g         1170 t
ORIGIN      
        1 ctttttgtct ggatgataca ttcaaaacat cgtgaccagc caaccgatga atcctcccat
       61 gattatcaga taggaagaat tcaccttgaa tcgagtgagg ccgataaaac tggcggcaaa
      121 aatgacgaca ctgacccaag agacgaaggt ggccatcccc aattgccaag atacggatgc
      181 catcaatgcc agcgaagcaa tcgtgattcc gtccaaaata acggagaaga tttcggatga
      241 tcgtagtcgg ttgaacagcg gatgcaacaa aagaatcagc aggaaggaag gcaaaaagat
      301 gccgacagtc gccaagatgg cacccgggac gccctgaatc agatagccaa caaaagtcgc
      361 cgtcgtgaag accgggcctg gggtgaactg gccgatcgca acggcatcca gcaactgtgt
      421 gctggtcagc gcagcatagc gctcgatgaa ttcagcctgt aaaaaagcca atagcacata
      481 gccgctcccg taaaggaccg atccgatttt caagaaagtc aggaacagca gcatcagcga
      541 catcggttcg atcgccagat gatgcgaccg ccacttggac acttgttgcg tcaccagcat
      601 cgtcagtccg gtcaggatga ggatcgtgat ttcgccgaca cccatcagcg acagcagata
      661 ggccgccaag aacaaaagca acgatttgct gtccttgatg gtcgtctgtc ccagcctaaa
      721 aagggcctgg atgatgactg caaggatgac cggtttgatg ccgctgaaaa gcgcttcgat
      781 gagcggcact tcgccgtact gtttatagaa catagccagc agcaacacga ttgtcatggc
      841 cggaaagatg aaacaaatgc ccgccaaaaa caatccgatt ttcccgccgc gcttgtagcc
      901 caacgcgata gccagttccg tagagttcgg accgggaatc agattcgtga atccgagcag
      961 atccatgaat tccgtctttt taagccattc ccgcttcgtc accaattcgt cctccatcat
     1021 ggcgatgtgt gcggctggac ccccaaaagc gaaaagaccc agtcgggcaa aaacgaagca
     1081 aatttccttg tagatttctt tggtataggt catgaggttc ctccatcaaa ggtatagttt
     1141 acagccggtt cagatgggtt cggtatagcc aacttgattt ttgaaaaggg tcaattcgtg
     1201 cacacccaaa tcctttaaca acgcgatggc atcgtcgaag tgatagcggt atttctggat
     1261 gctgtgcgca tctgaaccaa gactgaatcg ggtcccgccc atttccagat agaggccgat
     1321 catatagcgg tacaagtcgg cgttgttgta ttcatacatg ctgcgcgtgt tcaattccag
     1381 tgccatttct ttcgccatga tttcatcgag cagccccttc agcaaaggct cgaattcccg
     1441 aagatcgtct accgtgacgg gcaagcgacg gattccgtaa tcaaagtgcg cgaaaacatt
     1501 tgcgccatcg acttgccgga cagcttgcgt acagagctcg aaatattcct tcatgaccgc
     1561 tttggggtcc atctcatcga tgattggctg caagtaatca tagcgcccgt tttggtgaac
     1621 actcagcagg atgacatcaa aggctttgcc ttccagataa gctgtaattt gcgcgtggga
     1681 ctcaggcgta tagccgactt cgatgccaca gcggatgcgt ccatcgtgga ttgtgttcag
     1741 tgcgtctatt ttatccctgt aggtggcata atccaagaca gtatccacac cgccgttgta
     1801 ggcatcgtgg aaatccaggt gctccgttgt gacgagcagt ccgtctgttt gctcgagata
     1861 attttcaaat gattccgcag aatcgggcga aaaatgcgta tgcatatgtt gatcgtagta
     1921 acccaaaatc attcctcctt gtgcatggat gtcaccagta tagcagacag cgatggattt
     1981 ccagaaaaga ttccgttaag aaaatgtaaa ccatcgaagc cagaatatta aacattatca
     2041 aaaaatttgc gccaatgctt gtggaatttt gctataatga ttaaggtcgt ggaaaaataa
     2101 gataaatttt ttggattggt ggttttttat ttggcagcac gatatgtcat gggagtggat
     2161 gtcggtacca caagcacaaa agccgtactg tacgaaacgg acggctcagc ttatgaatca
     2221 gcgtacgcac attatccatt aattaaagag aatcccgaaa tggcggaaca agacttggat
     2281 gagatctttt cagccgtatt gaaaacaata aaagcagtca tacagaaagc tgaactgggc
     2341 ccgacggata ttgccgggat cggcttttcg agcgccatgc acagcttgat cctcatggac
     2401 gcggaaggaa cgccgttgac tcggagcatc acctgggccg ataatcgttc aaaaaaatac
     2461 gcaagcatgc tgaagcattc cggaatgggc cagcgttttt acgaaaaaac aggcacaccg
     2521 ctgcatccga tgtccccgat aagcaagatt ctttggctga aagcagaaaa gcccgaagtg
     2581 ttccaagcag cgcgttggtt catcggcatc aaggaataca ttctctggcg gctgttcggt
     2641 gaattcgtcg tggactattc cgttgcctcg gctaccggat tgttcaatat ccgggaattc
     2701 cgctgggatg aggaaattct ggaattctgt ggcatcacga gtcaaatgct gccgttgccg
     2761 gtaaatcctg gtaaacaact gagcggcctc tcggaggaag tggctgagtt gctggggctg
     2821 gctgcggata cgcccttcat agttggaggg aatgacggct gcttagccaa cttgggtatg
     2881 ggagcgattc gcgccggtac ggcaacaatc acgatcggta cgagtggtgc ggtccggatg
     2941 acgagtgaaa aaccttatgt aagcccggaa ggacgtacat tcagttatat cctggatgaa
     3001 agccattatg tgaacggcgg cgccgtcaac aatggcggaa acatctttga ttgggccatc
     3061 aaacaatttt tgccggccaa tgttgctgat gaggatctct acgacaaagc gatggagatg
     3121 attgcgaacg tcgcacctgg cgctgatggg ttggtttttc atccctatct gaacggagaa
     3181 cgggcgcctt tgtggaatgc ggatgccaga gggaatttca gcggcatcaa catgctgcat
     3241 acgaaagaac attttttgcg ctccgttttg gaggggatct gcctgaatct gaaggatgtg
     3301 ctggcaacgg tcatctctat gaacggcgaa ccaacaaaaa ttatggccag cgggggcttt
     3361 tcgcgctcgc tggcttggcg ccaaatcttg acggatatca tcggtatgcc gatcgaattc
     3421 cctgaaactt ttgaaagttc ctgtacgggt gccgctttga tcacgctgaa gagcttgggc
     3481 ttggtggaat ccgtggatga cgcagaaaag ttgatgggtg cttcaatcga acatcaaccg
     3541 aacgaagaca gcaagtccgt ctatgcggcg atgttcccgc gttaccagca gatgagcagg
     3601 ctcttgcagg aattttatac agaacaatga aatagaacca ataaacaaag gcaaggccac
     3661 tgagtgaaac tcagtggcct tgcctttttg tgcactcaaa gagggtttgg ttgttccagt
     3721 gcgctttcga tcagcttcgt cattttcttc ggtgccgtga tcggggcaaa acgcttgatg
     3781 atttcgccgt cgcggccgat gagaaatttc gtgaaattcc acttgatcgc accgcctccg
     3841 gtctgttcga ccagatattt gtaaagagga tctgcattct caccgttcac ttcgatttta
     3901 tcgaaaatcg gaaaagtgac gccataattg agtcggcaga attcagcagc ttcgactcca
     3961 ttcaaaggtt cttggttatt gaactgattc gatggaaatc ccaaaatgat gaaatccttg
     4021 tctttgtatt tttcgtaaag ctcctccagt tcatccaatt gccccaccaa accgcacttc
     4081 gaagctgtgt tgacgatgca gatgactttt cctttatact gttccaatga tacagatgac
     4141 tgatctgttt tagttgctga atattcataa atggacatat tgaaggactc ctttattatg
     4201 ctacttttga atgctaaaac atagtataaa caacaatgta aaggattgca aaagataagg
     4261 cccgcaaaaa agtgaaggag atatgacagt ttcatgagaa atgatgaaat tgtaacagta
     4321 aattccataa atgtctgttt tttttttcgt atttgctgta tgatagttga gtattataaa
     4381 tcgaatctgg agggattttt tttggacatg tttgaaggct taagccaaaa aatcgtagga
     4441 aaaaaaataa aaatcgtttt cccggaaggc ttagaaccaa gaatcttagg tgcagtcgta
     4501 cgtcttaaag cggaagactt agtggaacca atcgttatcg gaaatgtgga tgcagttaaa
     4561 gaagctgcaa aaggccgcgg attcaggcta aatggtatcc aaatcatcga tccaactgat
     4621 tatgctgaac tcgatgaaat ggttgcgtca tttgtcgctc gccgtaacgg aaaagtcact
     4681 gaagaacaag caaaaaaatt attgttggat gagaattatt
//
LOCUS       DAOMUJ010000093         4716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5064_length_4716_cov_1.872774, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000093 DAOMUJ010000000
VERSION     DAOMUJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5064_length_4716_cov_1.872774"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..>632)
                     /locus_tag="PLT73_03535"
     CDS             complement(101..>632)
                     /locus_tag="PLT73_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRM19487.1"
                     /translation="YLDTLERDKELLKKYTQLPYERISYDDAVALLNANGFDDIVWGD
                     DFGSPHETFIANQSDRPVFILNYPKAIKPFYMKEHPDRPEVVLCADMIAPEGYGEIIG
                     GSEREVDYQRLSEQIEKCGLSKEDYSWYLDLRKYGSVPHSGFGLGLERMVTWISGTEH
                     IRESIPFPRLLNRIYP"
     assembly_gap    633..642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<643..1381)
                     /locus_tag="PLT73_03540"
     CDS             complement(<643..1381)
                     /locus_tag="PLT73_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRM19488.1"
                     /translation="MDQAKAYVGQEISIGAWVTNKRSSGKIAFLQLRDGSHYFQGIVL
                     KNDVGQELFDVAKSLTQETSLIVKGIIQEDTRSKLGYELLVTGIEVLGESQDYPITPK
                     EHGTDFLMDHRHLWLRSSRQHAIMKIRNEIIRATYEFFNQEGFIKVDPPILTGSAPEG
                     TTELFHTKYFDEEAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMM
                     EPEMAFMHQDQSLEVQEKYVAYLVQSVL"
     gene            complement(1425..2615)
                     /locus_tag="PLT73_03545"
     CDS             complement(1425..2615)
                     /locus_tag="PLT73_03545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM19489.1"
                     /translation="MVKISERMKKISESPTLATSAKVNVMKAQGKDILNLTVGEPDFD
                     THASILNAAVEAIQSNKANHYTPTAGILPLRQAIVDYHQKYDGVTYAVNQVIVTEGAK
                     NALFALFQVILNPGDEVIVPAPYWVSYTEQIKLAGGVNVLVETAPKKGFKLTVEDLEK
                     HRTEKTVALLLNSPSNPTGMIYTPEELKAIGEWAVEHDVLIIADEIYYRLTYNGNNAI
                     SIASLSEEIKNQTIIINGISKTYAMTGWRIGYAIGNAEIISKMIELSSHSTSNPAGPS
                     QYGALAALTGPQDFVEEMRAAFEARLNFFYPLVASIPGFKVTKPQGAFYLFPNVKEAA
                     EMCGFKEVKDFTLALIEEANVALVSGDGFGYPDYARISYTVREELLAEAVDRIKKFIE
                     THKK"
     gene            complement(2631..3107)
                     /locus_tag="PLT73_03550"
     CDS             complement(2631..3107)
                     /locus_tag="PLT73_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HRM19490.1"
                     /translation="MKKNIIVGAIVILFLMIVSSYTIFYRSQQPMLQAQKEATAIAEE
                     NANIQKVQDFYWYNGSETYFTLAGIDGNNEELYVIIKKDGGETTVLNTAEVVTESEAK
                     SITQADKKPERILEARLGIENEEPVWEVTYKNANDTIGYYLISAISGKWLKDIENI"
     gene            complement(3205..4176)
                     /locus_tag="PLT73_03555"
     CDS             complement(3205..4176)
                     /locus_tag="PLT73_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRM19491.1"
                     /translation="MWPRAKALLDDLDTVQVNLQYFFQERAGTYHVLHFAWDKDLLSL
                     EIETNQIDYSGEIHSKIKDRFKRTVYLSSTLKVDKSLDYFKNKIGEAELQEGHFDSPY
                     NLSERLRIFLPDDITAITCLDDKRAASQIVLTLREIVLPQQRKALVLFTSNRLLEEVH
                     NQLKNVVSAEFAGTEILAQGFSGSRRRMHNRFMSADAAILLGSASYWEGIDFSDQAVE
                     ILVITRLPFDPPHRPENVAFQAYHQDQGKKAFYEEFLPRAMIRLKQGIGRLVRSRNAR
                     GVVICLDDRLIKSNYSKRMQLMLPEGVGIKVADHDEIKTEIQEFLKK"
     gene            complement(4184..>4716)
                     /locus_tag="PLT73_03560"
     CDS             complement(4184..>4716)
                     /locus_tag="PLT73_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19492.1"
                     /translation="LTFESHEAPLKGKWANDDFFAFNNKKAEQSSLIVTNHSFLSHHI
                     DDLAIFDQTESIDLILDEAHQFPRVYQDAHKKTVALSILKRRLKRFGYFLRDFREAIE
                     KDANGDSLDYDLFNLEFAIDQLYQECAYSEEVLMRSLIHEVGEKGEVYLDENFQLENG
                     MKNIYKKMPGQILLEE"
BASE COUNT         1143 a         1126 c         1018 g         1419 t
ORIGIN      
        1 gctaactcct ttaggaaaac agaaagcgga acaaatagct gctatttgcc ccactcccat
       61 tctgttttga ttgcattcag actcgtgaca tcccctgtta ttatgggtag attctgttca
      121 gtagacgagg gaacggaatc gactcacgga tatgttcggt tccggagatc caggtaacca
      181 ttctttcgag tcccaatccg aatccggaat gcggtactga accgtatttg cgcagatcca
      241 ggtaccatga atagtcttcc ttgctcaggc cgcacttttc gatctgttcg cttaagcgct
      301 gatagtccac ttcacgttcg ctgccgccga ttatttcacc gtacccttcc ggagcgatca
      361 tatccgcgca cagaacgact tccggacgat ccggatgttc tttcatgtaa aaaggcttga
      421 ttgctttcgg gtaatttagg atgaatacag gtctatcaga ctgatttgcg ataaaggttt
      481 cgtgaggcga gccgaaatcg tcaccccaga cgatatcatc aaatccgttt gcgttcaata
      541 aagctacagc atcgtcgtag ctgatgcgct cataaggtaa ttgtgtatat tttttcagca
      601 attctttgtc gcgctccagc gtatccaagt agnnnnnnnn nnccagcacc gactggacca
      661 gataagcgac atatttttct tggacttcca aactttggtc ctgatgcatg aaggccattt
      721 ccggctccat catccagaat tcgatcagat ggcggcgtgt cttcgatttt tcggcccgga
      781 aagtcggccc gaatgaaaat acttttccga aggccattgc agctgcttcc atatacaact
      841 gcccactctg ggacaagtaa gcttcctcat caaaatactt tgtgtggaac agctcggtcg
      901 tcccttccgg cgcactgccg gtcagaatcg gcgggtccac cttgatgaat ccttcttgat
      961 taaagaattc ataagttgcg cggatgattt cattacggat cttcatgatg gcgtgctgtc
     1021 tggatgaacg cagccataga tgacgatggt ccatcaggaa atctgttccg tgctctttag
     1081 gcgtgatcgg ataatcttgg ctttctccca gcacttcgat gccggttacg agcaattcgt
     1141 atccaagctt cgagcgggta tcttcctgga taatgccttt gacgatcaaa gaagtttctt
     1201 gcgtcagtga ttttgctacg tcgaacaact cttggccgac atcgtttttc aaaacgatgc
     1261 cttggaaata atgggagccg tcacgaagtt gcaggaaagc gattttcccg ctgcttcttt
     1321 tgttggttac ccaagcaccg atggaaatct cttgccctac atatgctttc gcttggtcca
     1381 ttgtgataag tgtcaatatg gtctactcct tttcccaaaa aaggttactt tttatgggtt
     1441 tcgataaatt ttttgatgcg atctactgct tctgcaagta gttcttccct gacagtgtaa
     1501 cttattctgg cgtaatcagg gtatccaaaa ccatcacctg aaaccaacgc gacatttgct
     1561 tcctcgatca gagccaatgt aaagtctttg acttctttaa acccgcacat ttcggcggct
     1621 tccttcacgt tcgggaacag gtaaaaagct ccttgaggtt tggttacctt aaaaccaggg
     1681 atgctcgcta cgagcggata gaagaagttc aggcgtgctt cgaacgctgc gcgcatttcc
     1741 tctacaaagt cttgcggtcc ggttaatgca gccaacgcgc catattggct cggtccggca
     1801 ggattgcttg tcgaatgact ggatagctca atcattttac tgatgatttc cgcatttccg
     1861 attgcatatc cgattctcca gccagtcatg gcgtaggttt tggatatacc gttgatgatg
     1921 atggtctggt tcttgatttc ttctgaaagt gaagcgattg agatggcgtt gttcccgtta
     1981 taagtcagtc ggtagtatat ttcatcggca atgatcaaga cgtcgtgttc aaccgcccac
     2041 tcgccgatcg ccttcaattc ttcaggcgtg tagatcatac cggtcgggtt cgacggcgaa
     2101 ttcaaaagca aagcaaccgt tttttcggtg cgatgttttt ccagatcttc cacagtgagt
     2161 ttaaagcctt ttttaggtgc tgtttcgaca agaacgttga cgccgccagc cagtttgatc
     2221 tgttcggtat aacttaccca gtatggcgca ggaacgatga cttcatctcc gggattaaga
     2281 atgacttgga ataaagcaaa caaagcattt ttggcccctt cagtcacgat gacctgattg
     2341 acggcgtaag tgactccgtc atatttctga tgataatcca caatcgcttg acgcaatggc
     2401 aggataccgg cagtcggggt gtagtggttt gctttgtttg attggattgc ttctactgcg
     2461 gcattcagaa tggaagcgtg cgtatcgaag tctggttctc ctacggtcaa gttaaggata
     2521 tcctttcctt gcgctttcat aacgttgact ttagctgacg tcgccaatgt aggggattcg
     2581 cttatttttt tcattctttc agaaattttt accatctcaa tcactccaat ttatatgttt
     2641 tctatatctt tgagccattt accagaaata gcggatatca aatagtaccc aatcgtatca
     2701 tttgcatttt tgtaggtgac ttcccaaacc ggttcttcgt tctctatccc cagtcttgcc
     2761 tccagaatcc gttcaggctt cttatccgcc tgagtgattg atttagcttc actttcggtg
     2821 acgacttccg cagtgttcag gacagtggtt tcaccgccgt ctttcttaat gatgacatat
     2881 aattcttcat tgttgccatc gatgccagcc agtgtaaaat acgtctctga tccattgtac
     2941 caataaaaat cttggacttt ttggatgttg gcgttctcct cggcaatggc ggttgcttct
     3001 ttttgcgcct gcagcatcgg ttgctggctt cggtaaaata tcgtgtagga actgacgatc
     3061 atcaggaaaa gaatcacaat cgccccaaca atgatattct tcttcacatc ttccacttcc
     3121 ttatctttgt ttgaaacctt attgcaatct cacctaatta ttaatgatac cttaaactgc
     3181 aagctgaggg aatatcccac cgcattattt tttcagaaat tcctgaattt ccgtctttat
     3241 ttcatcatga tcggctactt tgatccctac gccctccgga agcattagtt gcatacgttt
     3301 ggaataatta cttttgatca atctgtcgtc caggcaaatg acgacacctc tggcattcct
     3361 ggagcgcacg agtctgccga taccctgttt cagacggatc atcgctcgcg gcagaaattc
     3421 ttcatagaac gccttctttc cttgatcctg atggtaggct tgaaacgcaa cattttcagg
     3481 gcgatgcggg ggatcgaacg gcagtctggt gatcaccaga atttcgactg cctgatctga
     3541 aaaatctatg ccttcccagt acgaagccga gcccagcaag attgccgcat cggctgacat
     3601 gaatcgattg tgcatccttc ttcggctacc ggaaaatcct tgagccagta tttccgtccc
     3661 agcaaattca gcagatacaa catttttgag ttggttatgg acctcctcga gcaagcggtt
     3721 ggatgtaaac agaactaaag ctttgcgctg ttgcgggagt acgatttccc gcaacgttaa
     3781 aacaatctgg ctggctgctc gtttatcatc caagcacgta atggctgtaa tgtcatccgg
     3841 aaggaaaatc cgcaaccgct cacttaagtt atagggtgaa tcaaaatgcc cttcctgcaa
     3901 ttcagcttca ccgattttgt tcttgaaata atcaagcgat ttatccactt taagtgtcga
     3961 acttaaatat actgtccttt tgaatcggtc cttgatttta gaatggattt ctccggaata
     4021 atcaatctgg tttgtttcga tttccagtga aagcagatcc ttatcccaag caaagtgcag
     4081 cacatgatac gtacctgcgc gctcttggaa aaaatattgc agattgacct gtaccgtgtc
     4141 caagtcatcc agcaacgcct ttgcgcgcgg ccacatgacc ggatcattcc tcaagcagga
     4201 tttgtccagg cattttcttg tatatattct tcattccgtt ctcaagttga aaattctcat
     4261 caagataaac ttcgcctttt tctccaacct cgtggataag cgaacgcatc aatacttctt
     4321 ctgaataggc acattcctgg tacaattgat cgatcgcgaa ttccagattg aacaaatcat
     4381 agtccaaaga atcgccattg gcatcctttt ctatcgcctc tcgaaagtcc ctcaggaagt
     4441 agccgaatcg cttcaaacgg cgtttcagaa tggacaaggc taccgttttt ttgtgcgcat
     4501 cttggtaaac gcgcggaaac tgatgcgctt cgtcgagaat caagtctatg gactcagttt
     4561 ggtcaaaaat agccaaatca tcgatgtgat gactcaaaaa ggaatggttc gtgacgatca
     4621 gactggattg ttctgctttt ttgttattga aggcgaagaa atcatcattg gcccatttcc
     4681 cttttaaagg ggcctcgtgg ctctcaaatg tcagtt
//
LOCUS       DAOMUJ010000094         4699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5094_length_4699_cov_2.181525, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000094 DAOMUJ010000000
VERSION     DAOMUJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_4699_cov_2.181525"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1405)
                     /gene="fni"
                     /locus_tag="PLT73_03565"
     CDS             complement(338..1405)
                     /gene="fni"
                     /locus_tag="PLT73_03565"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRM19493.1"
                     /translation="MTLDSNRKDQHVDLAEKQYQLADLSDFGKMRFVHHSMPNLKVAD
                     ISLQTEMAGMSLAAPFYINGMTGGSERTKQINADLAAVAKMTGLAMASGSVSAALKDP
                     SVSDSFSVIRKVNPNGQIFANIGAHHSLENAKRAVDILEADALQIHINAPQEMIMPEG
                     DRDFTMWLRQIEEISAHVGVPVIVKEVGFGMSRETIGQLIDVGVHTIDVSGKGGTNFA
                     AIENARRTAITYDDLENWGQSTVISLLEASSHRGKAEFLASGGIKTPLDIVKALSLGA
                     KAAGLSGQFLHMVLSEGPEKTAETIEAWKQQITTVMAMLGKTSVADLARTDLIFQRDI
                     LDWCDMRGIDYRQYANRSVQS"
     gene            complement(1402..2517)
                     /locus_tag="PLT73_03570"
     CDS             complement(1402..2517)
                     /locus_tag="PLT73_03570"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HRM19494.1"
                     /translation="MTVTEASAPGKLFIAGEYAVVTPGHPSILVALDQFITVSLEEAD
                     TTGTIRSAQYGGLPIPWTREEDQLVIDQRENPFLYITEAVKVTERFIKESGIPLSYFH
                     LSVKSELDNASGKKYGLGSSGAVTVATIKALLKFYAIDPEFELVYKLASLAHLSVKSN
                     GSFGDIAASTYGGWIAYACFDREWIMEKATELTLTELLKTEWPQLMIRPLTPPANLRL
                     VIGWTGSPASTTHLVDRVNDVRGGIDDFYKTFLGASKTCVNAMIAAFEKGDLQAIQAG
                     IRENRLLLQQLTAHTGVVIETPPLTQLCDLAEKNLGAAKSSGAGGGDCGIVIIDRQSG
                     IIPLISEWEKVGIMNLPLHVFKYVRAAAPIEWKGASE"
     gene            complement(2541..3548)
                     /gene="mvaD"
                     /locus_tag="PLT73_03575"
     CDS             complement(2541..3548)
                     /gene="mvaD"
                     /locus_tag="PLT73_03575"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRM19495.1"
                     /translation="MSNQAYVRAHTNIALIKYWGKRDEIDFLPMNSSLSMTLDKLYTD
                     TMVVFDDSFAQDSFYLNGEKQGEKETKKISKFLDLFREEKKTAMRARVESINHVPTAA
                     GLASSASAFAALAGAANLALGLDLDPQRLSTFARRGSGSATRSIYGGFVEWDMGTCSD
                     DSFAVPVDDASWDIGMVIVAVNQKAKEVSSRIGMKRTVETSPFYPAWVESAKKDIQDI
                     KAAIAAKDFIRLGEITEANGMKMHGTMLGAEPPFSYWEPDSIVAIKTAQTLRKQGIPC
                     YVTMDAGPNVKVLCRLSQAETIKQALLDHFTEDKLIITKPGKGIRELTAAERAVYNWN
                     D"
     gene            complement(3541..4494)
                     /gene="mvk"
                     /locus_tag="PLT73_03580"
     CDS             complement(3541..4494)
                     /gene="mvk"
                     /locus_tag="PLT73_03580"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRM19496.1"
                     /translation="MTNTRCSTGTSHGKIILMGEHAVVHGEPSIALPFPAVEMTATVC
                     EAKGPLTIDCSYYNGIAAEMPEILESMRTAIHSALAELQVSDENLAISLASTIPAERG
                     MGSSAAVSVALTRALFAYFDKPLNEATLLRLVNISEKVAHGNPSGLDAATASGDAPLF
                     YIKGQPFEAFPLNLKAYLIVGDTGVTGQTKEAVSSIAAKLNGKNPQPYRDAISRLGTF
                     ALTAKSAIEANDPSQLGELMNQAHAVLSFLDVSSPDLDRLVSAARFAGALGAKLTGGG
                     RGGCMIALARNRSEAEKIEEAIRQAGAIRTWIYQMGGTEDV"
BASE COUNT         1034 a         1227 c         1207 g         1231 t
ORIGIN      
        1 gtttagggga aaggctcatc caactgcgga tgggccttat cagtcgggtt ttttgtgatt
       61 tctggtaatc attttgtcga cagatgcgga atctctcgat agtttggaaa cccatcccgg
      121 gcgtacgccc gacagaagtg ccatctgtcg ggtttcggaa ctgaaatgca ggtcgtacac
      181 ctgacgggtg gtcaaactgt cgacttaacg ttccccaatg cttacgtaac acctgacatt
      241 ataaggattc tgtccagaaa atcggctata caaataaaat aaaaacgcgc cagcaaacgg
      301 tatcactcta ccggtcgctg gcgcattcgt attatattca actttgcact gatcgattgg
      361 cgtactgtcg gtagtcgatc ccgcgcatgt cgcaccagtc taggatatcc cgttggaaaa
      421 taaggtctgt ccgagccaga tcggcaaccg acgttttgcc cagcatggcc atcactgtcg
      481 tgatctgttg cttccaagct tcgatcgttt cggcagtctt ctccggacct tcgctcagta
      541 ccatatgcag gaactgaccg gaaagtccag ctgcttttgc acccaagctc agcgctttga
      601 cgatatccaa cggtgttttg atgccgccgg atgcgagaaa ttccgctttc cctcgatgcg
      661 aagaggcttc cagcaacgaa ataacagtcg actggcccca attttccaaa tcgtcatagg
      721 taatcgctgt acgtcttgcg ttttcaatcg ctgcaaagtt cgtgccgcct ttgccggaga
      781 catcaatggt atggacacca acgtcaatca gctgtccgat cgtttcccgg ctcataccga
      841 aaccgacttc cttcacgatg acggggacac cgacatgggc gctgatttcc tcgatctggc
      901 gcagccacat cgtgaaatcg cggtcgcctt ccggcatgat catttcctgc ggtgcattga
      961 tgtggatctg caaagcatcg gcttccagaa tatcgacggc gcgcttggca ttttcgagcg
     1021 aatggtgggc gccgatattc gcgaatatct gtccgttcgg atttaccttg cggatgaccg
     1081 aaaagctgtc gcttacgctg ggatccttca aggctgcgct gacggaaccg gaagccatcg
     1141 ccaagccggt catttttgcg accgcggcca gatcggcatt gatctgtttt gtccgttcgc
     1201 tgccgccggt catgccgttg atgtagaagg gcgcagccag gctcataccg gccatctcgg
     1261 tctgaaggga gatatccgcc actttcagat tgggcataga gtgatgaacg aagcgcatct
     1321 tcccgaaatc ggaaagatcg gctagttgat attgtttttc ggccagatcg acgtgctggt
     1381 cttttctgtt tgaatcaagc gtcattcgga tgctcctttc cattcgatgg gtgcagcggc
     1441 gcgtacgtac ttgaatacat gcagcggcaa gttcatgatt ccgacttttt cccattccga
     1501 aatcaaaggg atgatgcccg attgtctgtc gatgatgacg ataccgcagt caccgccacc
     1561 ggcaccggat gattttgccg cacccaggtt tttctcggcc aaatcgcata attgcgtcaa
     1621 ggggggcgtc tcaatgacga caccggtgtg cgcagtcaat tgctgcaaca gcaagcggtt
     1681 ctcccggatg cctgcctgaa tcgcctgcag atcgcccttt tcgaaagcgg cgatcatagc
     1741 gttcacgcac gttttactgg cacccaggaa tgttttgtaa aaatcgtcaa tgccgccgcg
     1801 gacatcgttg acgcgatcga caaggtgggt tgtggaagcc ggcgagccgg tccagccgat
     1861 caccaaccgc agattggctg gtggtgtcaa tggccggatc atcaactgcg gccattctgt
     1921 cttcaacaac tcggttaacg tcagctcggt tgccttttcc attatccatt ccctgtcgaa
     1981 acaagcgtag gcaatccagc cgccgtaggt acttgccgcg atgtcgccga aggaaccgtt
     2041 actcttgacg gacaaatgcg ccaaggatgc cagcttgtag accaattcga attccgggtc
     2101 gatcgcataa aatttcagca atgctttgat ggtcgcgacc gtcactgcgc cgctcgatcc
     2161 aagtccgtat tttttcccac tggcgttatc caattcgctc ttcacagaga gatggaagta
     2221 agataacgga atcccggatt ctttgatgaa gcgttccgtc accttgaccg cctcggtaat
     2281 gtagaggaac ggattttcgc gctggtcgat gaccaactgg tcttcttcac gggtccaagg
     2341 tatcggcaac ccgccgtatt gtgccgacct gatcgtcccg gtcgtatcgg cttcctccaa
     2401 agaaaccgta atgaactgat ccaaagcgac caaaatcgat gggtgccccg gcgtcactac
     2461 agcatattct ccggcaatga acaatttacc cggagcactt gcttctgtta cagtcatgaa
     2521 ttcaaccctc tctgtttctg ttagtcgttc caattgtaga cagcgcgttc ggctgcagtc
     2581 aattcccgga tgcctttgcc gggcttggtg atgatcaatt tatcttctgt gaaatgatcc
     2641 agcaacgctt gttttatcgt ttcggcttgc gaaaggcggc acagcacttt cacattcgga
     2701 ccggcatcca tcgtgacata gcaagggatg ccttgtttgc gcaaagtttg tgctgtcttg
     2761 atggcgacga tgctatcagg ttcccaatag gaaaatggcg gttctgcccc caacatggtt
     2821 ccatgcattt tcatgccgtt cgcttcggtg atttcgccta agcggatgaa atctttcgca
     2881 gcgatggctg ctttgatgtc ctggatatct tttttcgccg attcgaccca agccggatag
     2941 aagggagaag tttcgaccgt acgtttcatg ccgatgcggc tggagacttc tttcgctttc
     3001 tggttgaccg ccacaatgac catgccgata tcccagctgg catcgtcgac cgggactgcg
     3061 aaggaatcgt cggaacaagt ccccatgtcc cattccacaa aaccgccgta gatgctgcgg
     3121 gtcgcactgc cgctgccgcg tcttgcgaaa gtcgagaggc gttggggatc cagatcaagt
     3181 cccaaagcta agttggcagc tcctgccaaa gcagcgaagg cggacgcgga ggatgccaag
     3241 ccggctgcgg tagggacatg gttgatgctc tctacgcgcg cacgcatggc tgtctttttt
     3301 tcttcacgga acagatcaag gaacttggag atttttttgg tttctttttc gccttgcttc
     3361 tcgccgttca gatagaagga atcctgcgcg aacgaatcat cgaagacaac catcgtatcc
     3421 gtgtatagtt tgtccaaggt catggaaagg ctgctgttca taggcaaaaa atcgatttcg
     3481 tcccgctttc cccaatattt gatcaacgcg atgttagtat gcgctcggac ataagcttga
     3541 ttagacatct tcagttcctc ccatttgata gatccaagtc cggatcgcac cggcttgacg
     3601 gatagcttct tcaatttttt ccgcttcgga gcggtttctg gccaaagcga tcatgcaacc
     3661 gccgcggccg ccgccagtta atttggcgcc taacgctcct gcaaatctgg ctgctgaaac
     3721 gagtctgtcc agatcagggc tggatacgtc cagaaaagac aagactgcat gagcctggtt
     3781 catcaattcc cctaattgtg acggatcatt tgcctcgatc gcggattttg ctgttagagc
     3841 aaaagtgccc aatcgggaga tggcgtcccg gtatggttgc ggattttttc cattcagttt
     3901 cgctgcgatg ctggatactg cttcctttgt ttggccggtc acaccggtat cgccgactat
     3961 caaataagcc ttcaggttca aagggaaagc ttcgaaaggc tgacctttta tgtaaaagag
     4021 cggtgcgtcg ccgcttgccg tggcggcatc cagaccgctg gggtttccgt gtgcaacttt
     4081 ttcggatata ttgacgagcc ggagcagggt cgcttcattc agcggcttat cgaagtaggc
     4141 gaaaagggcg cgggtcaagg ccactgatac ggcagcgctg gaacccatgc ctctttcggc
     4201 cgggatggtg ctcgcgagcg aaatcgctag gttttcatcc gaaacctgca gttcggccaa
     4261 tgccgagtgg atagctgtcc gcatgctttc caggatttca ggcatttctg cagcgatgcc
     4321 gttataatag gaacaatcaa tggtcaaggg acctttcgct tcgcatacgg tcgcggtcat
     4381 ttcgactgcc ggaaagggta gtgcgatgga aggctctccg tgtacgacag cgtgttcacc
     4441 catcaggata attttgccgt gcgatgtgcc ggttgagcaa cgtgtatttg tcatagagta
     4501 tcgtccttta ctgattctta cgcgtcttct attatgcttg ctgtacatga atttggcaaa
     4561 tcgccattat ttagtcgaaa cttttggatt tgtgaaggtg ccgcatgtgt aatgacgaca
     4621 aattcagcac atatttttcg ctattttcta tcatcgtttt cattgtatcg gagcaaaaat
     4681 gcaaagtaaa tcaaatctt
//
LOCUS       DAOMUJ010000095         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5123_length_4674_cov_2.244858, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000095 DAOMUJ010000000
VERSION     DAOMUJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_4674_cov_2.244858"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLT73_03585"
     CDS             <1..760
                     /locus_tag="PLT73_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19497.1"
                     /translation="TQGYQRFGYEVWWICGTHYRPGGIKITQSICQFLNHSDALGNWI
                     CILDTAANEIAIHHHLKLSSDDRFHFMKIAIPVDDLKVVALESLYAKGCLPGYKDLLL
                     PSAGKIVDRKHMQAKDINLLRYRTDPEHRKFLLAVYLDRKDLYHLPAFLFELPCRTLG
                     IRTPAYIWRYYLLKDFSNPETAKLLTKKHLLEWLSTCIRTNRIRTRVAMEANMELLWE
                     PLLQFMMRLVEDGHAAAIGREAWVLLSERIIIRV"
     gene            849..2666
                     /gene="pepF"
                     /locus_tag="PLT73_03590"
     CDS             849..2666
                     /gene="pepF"
                     /locus_tag="PLT73_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRM19498.1"
                     /translation="MTNTEKHLKRAEVPEASTWDLTAIFPTDAAFEKELDELAGAVPF
                     IRALQGTIGSSSQALLKGIEEVLEINQKLERVYVYSHLKNDQDTADSLYQNLHDKALS
                     ILTSVSEATSWFEPEIMEIPEDELEKYLAENDKLKPYAHLLDSLTSAREHVLTAREEE
                     LLAGAGEIFAAPSKTFSVLNNADMTFPKVKNEEGQEVQLTHGLYGKLMESPDREVREG
                     AFKALYKTYDGLKNTFATTLSTNVKAHNYKAKVHKYESARAAALANNHIPESVYDTLL
                     DVVNEHLPLLHRYVDLRKDLLGVEELHMYDMYTPLTGEAPIKYSIEAAMEETLKGLQP
                     LGEDYLAILQEAFDNRWIDWLENEGKRSGAYSSGAYDTNPYILMNWQDSLNQLYTLVH
                     ELGHSVHSYLTRKNQPYVYGDYSIFLAEIASTTNENILTDYLLKTQTDPKVRIYILNH
                     YLDGFKGTIFRQTQFAEFEHYIHQQALAGVPLTQDFMTSYYAELNAKYYGPSVEKDPE
                     IGIEWTRIPHFYYNYYVYQYATGFSAATALAKRIVEGEEGALEKYLSYLKAGNSDYPI
                     EVMKKAGVDMTQSAYIEDAMKVFEERLNELEQLIAAQQA"
     gene            complement(2744..3442)
                     /locus_tag="PLT73_03595"
     CDS             complement(2744..3442)
                     /locus_tag="PLT73_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRM19499.1"
                     /translation="MYRSKQNTADSISSPQQIFELYLFVNPIGHKCYLTEQEVLKFMS
                     SLEGPKVHFRFITFHNFNTVTQYMKRLNLPEKDLTLRNQIYSSIYDASLAYKAALMQG
                     KKRGRNFLLHLQKSLADGKTAYSKELLTVVAIKANLDVDMFFEDKESPFVKSSYEADQ
                     HIAQEMNIKTNPSLVIFDNLNYQYGLLLEDNITAEVLHEVIKDCSVACDQKNANLKKT
                     SITTIGKNYQRITH"
     gene            complement(3809..4402)
                     /locus_tag="PLT73_03600"
     CDS             complement(3809..4402)
                     /locus_tag="PLT73_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRM19500.1"
                     /translation="MSQNIEKEFKNLLNKEEYEALIVAFDLDETEPTKQTNIYFDTPD
                     FKLKGLNSGLRIRMYEDKGEITLKTPIQENEKLETNDDLTLDEAKTLVEAHLMKATGN
                     VADKLKGLGIAPGDLVIIGQLSTIRYDFPGDKGTFFLDKSFYQDQMDYELEFESESLE
                     EGALIFQNFLKLHDIKVRKAKQKIERMLAYPNSTTHH"
BASE COUNT         1273 a          940 c         1082 g         1379 t
ORIGIN      
        1 tacacagggg taccaaagat tcggctacga agtgtggtgg atttgtggaa cgcattatcg
       61 accggggggc atcaaaataa cgcagtcgat ctgccaattc ttgaatcatt ccgatgcact
      121 cgggaattgg atttgtattc tggatacagc agcgaatgaa atagccatcc atcaccatct
      181 aaagttgagc agcgatgatc gctttcattt tatgaagatt gcgattccgg tggatgatct
      241 taaagttgtt gctttagaaa gtctttacgc aaaaggttgc ttgccagggt acaaagattt
      301 gctgctgccg tctgccggca aaatcgttga ccgcaaacat atgcaggcga aggatatcaa
      361 tctgttgcgg tatcgaaccg atccggagca tcggaaattt cttttggcgg tctatctcga
      421 tcggaaggat ttgtatcatt tgccggcatt cctgtttgag ttaccttgtc gaacgttagg
      481 catccgaacg ccggcctaca tctggcgtta ctatcttctt aaagacttca gtaatccgga
      541 aacggcaaag cttctcacaa aaaaacacct tctcgagtgg ctgtcaactt gtatacgcac
      601 gaacagaatc cggacacgtg ttgcgatgga ggccaacatg gaacttctat gggaaccctt
      661 gctccaattc atgatgcggt tggtcgagga tggccacgcc gcagccattg gcagggaagc
      721 atgggtcctg ctgtccgagc ggatcatcat tcgtgtttga gttagaatct gtatattctt
      781 gccagtaccc tttcggccct aaggtatgct aaaattatac tattgaaata attttagggg
      841 gaaagtggat gacaaacaca gagaaacatt taaagagagc ggaagtccct gaggcttcca
      901 cgtgggattt gacagcgatc tttccgacgg atgcagcttt cgagaaagag ttggatgaat
      961 tagcgggagc ggttcctttc atacgggctt tgcagggaac catcggctcg agcagtcaag
     1021 ctctattgaa agggattgaa gaagtcctcg aaatcaatca aaagttggaa cgggtctatg
     1081 tctattccca tctgaagaat gatcaggata cggcggacag tctgtatcag aatttgcacg
     1141 ataaagcctt gtcgattttg acgagcgtca gtgaagctac ctcttggttc gagccagaaa
     1201 tcatggagat tccggaggat gaactggaga agtacttagc agaaaacgat aagctgaaac
     1261 cttacgcgca tttattggat agccttacct ctgctcgtga gcacgtcctt accgccagag
     1321 aagaagagtt attggcgggt gcgggagaaa tttttgctgc gccaagcaag actttttccg
     1381 ttctcaataa tgctgacatg acattcccga aagtgaaaaa tgaagagggc caggaggtcc
     1441 aattgactca tggtttgtat ggaaagttga tggaaagccc ggatcgggaa gtccgtgaag
     1501 gagccttcaa agcactatac aagacatatg atggtctgaa aaatacgttt gcgactacct
     1561 tatcgactaa cgtgaaggcg cacaattaca aagccaaagt acataaatat gaatccgccc
     1621 gtgcagccgc tctggcgaat aatcacattc cggaatccgt ttacgacacg ttgttggatg
     1681 tggtgaatga acatctacct ttgttgcatc gctatgtgga cttgcgtaaa gacttgttgg
     1741 gggtcgagga gttgcatatg tacgatatgt atacgccatt gacaggggaa gccccaatca
     1801 aatacagcat cgaggcggcg atggaagaaa ccttgaaagg tcttcagccg cttggagagg
     1861 actatttggc tatcctccag gaagcatttg acaatcgttg gatcgactgg cttgagaatg
     1921 aggggaaacg cagcggagct tattcttccg gtgcctacga cacgaatccg tatatcctga
     1981 tgaattggca agattcgttg aatcaattgt atacattggt ccatgaattg ggacacagcg
     2041 tgcacagcta tctgacaaga aagaaccagc cttatgtgta cggagattat tcgattttcc
     2101 tggccgaaat cgcttcgaca acgaatgaaa atattttgac cgattatttg ttgaagacgc
     2161 aaaccgatcc taaagtccgc atctatattt tgaatcatta cctggatggt ttcaaaggaa
     2221 cgatattccg ccaaacccaa tttgccgaat ttgaacacta tatccatcag caagccttgg
     2281 caggtgttcc gttgacgcaa gatttcatga cttcttatta tgcggaattg aatgccaagt
     2341 attatggtcc gagcgtcgaa aaggatcctg aaatcggcat cgaatggacc cgtattccgc
     2401 atttctacta taattattac gtctaccaat acgctaccgg cttttcggct gcgacggcat
     2461 tggctaaacg catcgtggag ggtgaagagg gcgcgttaga gaagtacctt tcctatctga
     2521 aagcaggaaa cagcgactat ccgattgaag ttatgaaaaa agcaggcgtg gacatgactc
     2581 agtcagctta catcgaggat gctatgaaag tgttcgagga acgtctgaat gaactcgaac
     2641 aattgattgc agcgcaacag gcttaatcaa atgagtcctt ctaataatag tcttcattag
     2701 aaaaagcttc gcggccctcc gagggtcgcg aagcttttct taatcagtgg gtgatgcgtt
     2761 ggtagttttt tccgattgtc gtgatgcttg ttttcttcag gttggcattc ttctggtcgc
     2821 acgctaccga gcaatccttg attacttcat gcaatacttc ggcagtgatg ttgtcttcca
     2881 acaagaggcc gtattgataa ttcagattat caaagatgac aagcgaagga ttcgttttga
     2941 tgttcatctc ttgggcgata tgctgatctg cttcatagct gctttttacg aaaggcgatt
     3001 ctttgtcctc aaaaaacatg tcgacatcca gattagcttt gatagccact accgttaata
     3061 gttctttaga gtaggcggtc ttaccgtccg caagtgattt ctgcaggtga agcaaaaagt
     3121 tgcggccgcg ttttttgcct tgcatcaagg cagccttata tgccaaagag gcatcataga
     3181 tggaactgta tatttgattg cgtaaggtta aatctttttc cggcaaattt aatctcttca
     3241 tgtactgcgt cacggtattg aaattatgga aagtgatgaa gcggaagtga actttcgggc
     3301 cttccaatga actcatgaat ttcagaactt cttgttcggt aaggtagcac ttgtggccta
     3361 tcgggttcac gaatagataa agctcaaaaa tctgttgagg agaagagata gaatcagctg
     3421 tattctgttt tgacctatac attttaatcc tccctctttc ctgttttgct tacttttagt
     3481 ttaccaaaaa aaatcccaaa caagtagcaa taaggcttcc cgaagagttg cctgttgaag
     3541 accggcaata gttcttcctt atgaccttct tttttcttgt tcagtatcgg ctgtttcatt
     3601 gtcaaggtgt attttacgag acaatgaact aatttgcaac taaatcgatt gattttttta
     3661 tcttgtatag cttgaaattc gattcaaaca gaagaatttt gcgcttttgt ctgttttacg
     3721 cgatgaaata agcaaaaaga tccattcttc gttcgattct gcggagaatg gatcttttta
     3781 tatagaatgg atcggcggaa attaactact aatgatgtgt cgtcgaattt ggataggcca
     3841 gcatacgttc gattttttgt ttcgcttttc ggactttgat gtcatggagc ttcagaaaat
     3901 tctggaatat gagggctcct tcttccaaag actcggactc gaattctaat tcgtaatcca
     3961 tctgatcctg gtagaaactt ttatccaaga agaaggtacc tttgtcaccc gggaaatcat
     4021 agcggatggt cgagagttgt ccgattatga caaggtcccc tggcgcgata cctaatccct
     4081 tcaatttgtc ggctacattc cctgttgctt tcatcagatg agcttcgacc aatgtttttg
     4141 cttcatcgag agtcaaatca tcattcgttt ccaatttttc attttcttgg ataggcgtct
     4201 tcagtgtaat ttcaccttta tcttcataca tccggatgcg cagtccactg ttcagaccct
     4261 tcagtttaaa gtcaggtgta tcgaaataga tattggtttg tttggtcggt tcagtttcat
     4321 caaggtcgaa ggcaacgatc aacgcttcgt attcttcttt atttaatagg tttttgaatt
     4381 ctttttcaat attttggctc attgtcggta cttcctttct gttaagaggg tcgttttgat
     4441 cgcggtagct gatcaaaatt cgaatgtgcg ttaaatccat tcatcagcac ataatcgagt
     4501 aggtgatgca aaatcttaat catattgtat cataagtttt gcattggtta tcatttagcg
     4561 atactgatgt aaagtgcgta ttgtttatat ttgaataaaa aatgaattcg tcccttttcg
     4621 acaagaattg tatagaatca tgcctagaga acctagaaaa tagatttgaa gata
//
LOCUS       DAOMUJ010000096         4668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5134_length_4668_cov_1.817472, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000096 DAOMUJ010000000
VERSION     DAOMUJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5134_length_4668_cov_1.817472"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="PLT73_03605"
     CDS             complement(<1..2226)
                     /locus_tag="PLT73_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRM19501.1"
                     /translation="MAKRTTTTRKKKKATGQKISYELIGVLFLFSAALGIGQLGFAGI
                     ALANFFRFFVGETYPVSLALFGAYGLYLILRGKEPKIRKNWLISGILLYSAALLYLHS
                     QAFMTVVAEGASVVSLTLARFLTDVRQADTASEMGGGLIGAAFYSGTYFLVSQWGTYI
                     IIGLLVFFGVAVLFGFTTHDVMEIVRKVALIFGHNSKRAVLYTKDKVASSIIDSKTVK
                     KPTRSNSVPVDKKPSIVEKAKNQIKSESAAKQLMKEEAEALKASEKETLSAKEHEPVQ
                     LKIDSFQQQLEKAAEHKHADNEKSASSQQASLAKTTAAGGEEDNNSELEFEITAEQEN
                     RDYHLPPVTLLNEIKAIDQSNEYATIEKNVKKLEETFASFGVEAKVTKANLGPAVTKY
                     EIQPAVGVKVSKIVSLSDDLALALAAKDIRIEAPIPGKSFIGIEVPNSEVSVVSFRDV
                     MEGQKHTDKLLEVPLGRDISGVVQSANLTKMPHLLIAGATGSGKSVCINGIITSILMK
                     AKPNEVKMMMIDPKMVELNVYNGIPHLLTPVVTNPRKAAQALHKVVTEMERRYELFAG
                     TGMRNIDGYNNLIVEYNLENGENNPTLPYIVVIVDELADLMMVASNEVEDAITRLAQM
                     ARAAGIHMILATQRPSVDVITGIIKANVPSRIAFAVSSSIDSRTIIDGSGAEKLLGRG
                     DMLFRPMGENKPIRVQGAFISDEEVEHIVTFVKNQQEANYVEEMMPTETVATASDEPE
                     DD"
     gene            complement(2436..2732)
                     /locus_tag="PLT73_03610"
     CDS             complement(2436..2732)
                     /locus_tag="PLT73_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HRM19502.1"
                     /translation="MYQVIRTEGEYEPWWFFEDWMDFIVESTDYADFQEAVAAYERAA
                     EKLSVVYPFQKTKETYLTAYWSDGEIRFCENCDDDVQLYHGLMLLQDGKKLLQE"
     assembly_gap    2931..2940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2983..3258
                     /locus_tag="PLT73_03615"
     CDS             2983..3258
                     /locus_tag="PLT73_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRM19503.1"
                     /translation="MQRVRIRVAGVVQGVGFRYMTKMVADQIGITGIVRNEADGSVYI
                     EAVGPGDKIDTFIAAVKRSPSPSGKVDSCSILEDANIENYISFRVTN"
     gene            3321..4253
                     /gene="yidC"
                     /locus_tag="PLT73_03620"
     CDS             3321..4253
                     /gene="yidC"
                     /locus_tag="PLT73_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRM19504.1"
                     /translation="MKLKKKLLLSAEMMALLVVLTGCMQYDENKNPTGFIYDYLVVPT
                     GNLIVMLAEMLNGNYGLAIIAITIIVRLAIMPLNFSQIKKTMVQQEKMKYIKPELEDI
                     QFRQKNAQSPEEKAAVSQEMMALYKENNISMTGGVGCLPILIQMPIFTAMYQAVNLTP
                     EISQSTFLGINLGVSSPLLAILAGVAYVIQGYVSTIGMPQETKSQMKSMMLMNPIMIL
                     MFSWSSPAGLALYWLAGGIFAAAQTALQNHMIKPKIQKQVEEEMKDRPIKKNVKMAKP
                     ASQSTSTQSVKALPAKNQQTGGKKGRNAGKQTRS"
     gene            complement(4363..>4668)
                     /locus_tag="PLT73_03625"
     CDS             complement(4363..>4668)
                     /locus_tag="PLT73_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013727998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM19505.1"
                     /translation="STDRHEIHLSISESMSYVQIAIQDFGEGMSQEDQQKIFSRFYRV
                     DKARSRNKGGNGLGLSIAKELLEGYKGDITVESVLGHGSVFRIQLPILKNYVPDEDL"
BASE COUNT         1205 a         1163 c         1016 g         1274 t
ORIGIN      
        1 gtcatcttcc ggttcatcgc ttgcagttgc caccgtttca gtcggcatca tttcctcgac
       61 atagtttgct tcttgctgat ttttgacgaa agtgacaatg tgctccactt cttcgtcgga
      121 tatgaacgcc ccttgcacac gaatcggttt attttccccc atcggacgga agagcatatc
      181 gcctctgccc aataatttct ctgccccgga gccatcgatt atcgttctcg aatcaataga
      241 actggacacg gcaaatgcga tccgggaagg aacgtttgct ttgatgatcc cggtgatgac
      301 atcgacactt ggtcgttgcg tagccaaaat catatggatt cccgccgctc gcgccatctg
      361 agccaatcgg gtgatcgcat cttcaacctc attacttgct accatcatca aatcggctaa
      421 ttcatccacg atgaccacta tatatggcaa tgtgggatta ttttctccgt tttctaaatt
      481 atattcgaca atcagattgt tgtatccgtc gatgttgcgc ataccagtac cggcgaatag
      541 ttcatatctt cgttccattt cggtcacaac tttatggagc gcttgcgccg ctttgcgcgg
      601 atttgtgaca actggtgtca gcaaatgagg aattccgtta tagacattca gttcgaccat
      661 tttcggatca atcatcatca tcttcacttc gtttggcttg gctttcatca atatgcttgt
      721 gatgatgcca ttgatgcaga cggattttcc gcttccggtc gcacccgcaa tcagcagatg
      781 gggcatcttc gtcagattcg cactttgtac gacgcccgag atatcccggc ctaatggtac
      841 ttccaacagc ttatcggtgt gcttctgacc ttccatgaca tcacggaagg agacgacgct
      901 gacctcactg ttcggtactt ctataccaat gaaggatttt ccgggtatcg gcgcttcgat
      961 acggatatct tttgctgcca aagctaaagc caaatcatca ctgaggctta caattttgct
     1021 gaccttcacc cctacagctg gttggatttc atatttggtc acggcaggcc ccaggttcgc
     1081 tttggtgact ttggcctcca ccccgaaact cgcgaacgtc tcttccagct ttttgacatt
     1141 tttttcgatt gtcgcatact cattcgattg atcgattgcc ttgatttcat tcaacagggt
     1201 gaccggtggc aaatggtaat ccctattctc ctgctcggca gtgatttcaa actccaactc
     1261 actattgttg tcttcctcac cgcctgcagc cgttgtcttt gccagactag cctgctggga
     1321 agacgcgctc ttttcattat ccgcatgttt gtgttcggct gctttttcaa gttgctgctg
     1381 gaaactgtcg attttcagct gaacgggttc gtgttctttc gcggagagtg tttccttttc
     1441 tgaagctttc agagcttccg cttcttcctt catcaattgc ttcgcggccg attcgctttt
     1501 gatctgattt ttagcttttt cgactatcga aggcttcttg tcgaccggaa cactatttga
     1561 tctggtcggt ttcttcactg ttttcgaatc gatgatcgag ctggccactt tatctttcgt
     1621 gtaaagcacc gctcgtttgc tgttgtggcc gaagatcaaa gcaactttgc gcacaatttc
     1681 catgacatca tgcgtcgtaa agccgaatag gaccgccaca ccaaaaaata caagcaggcc
     1741 gataatgatg taggtgcccc attgtgaaac caaaaaatag gttccgctat aaaaggctgc
     1801 cccgattaag cccccgccca tctccgaagc cgtatcagct tgcctgacat cggtcaaaaa
     1861 acgtgcaagc gtaagggaca cgaccgacgc gccctccgca acgactgtca taaaagcctg
     1921 tgaatggaga taaagcagcg ctgctgaata aagcaggatg ccactgatca gccagttctt
     1981 tcttattttt ggttctttcc ctctcaaaat caggtacaga ccataggcac cgaagagcgc
     2041 caagctgacc ggataggttt caccgacaaa aaaacggaaa aaattcgcca gcgctatacc
     2101 ggcaaaaccg agttgcccga tccctaaggc tgcactaaaa agaaacagca cgccgatgag
     2161 ctcgtaagat atcttttgcc cggtggcctt ctttttcttt cttgttgtcg tcgttcgttt
     2221 cgccaaacat gccaactcct tttttatctt tactcaaaac cactgcccgt ttgcataaaa
     2281 cagcggtcca tagattgttc gtttgattac acgtatcggt tcattatact acaaaatgaa
     2341 cccttttcga ggctattttt taatcaatcc acgcggacaa aaagcttctg ttttccgtca
     2401 atgcgggcaa cagaagcttt ttggaatttg gtcgatcatt cttgaagaag ttttttccca
     2461 tcctgcagaa gcatcagtcc atgatatagt tgcacatcgt catcgcaatt ttcacaaaaa
     2521 cggatttctc cgtctgacca gtaggcagtc aggtaggttt cttttgtttt ttggaatggg
     2581 tagacgaccg ataatttttc cgcagcccgc tcataggcag cgactgcctc ctggaaatcg
     2641 gcgtagtctg tcgattccac aatgaaatcc atccagtctt caaaaaacca ccaaggctca
     2701 tattctccct cagtccgtat cacctggtac acgctatcac cttctttgtt gcaaaaagta
     2761 tagcacagct cgaaaaaaaa ttcccgcgca aggtatcgtg attgaaactt cttggcgaag
     2821 acggtaaact gaaattacaa gttgcaccgt atgcttatcc tagaatctgg aggtaatctg
     2881 aatgaaaaaa atcaagatga ccgtaaacgg tcgggtacag ggcgtcgggt nnnnnnnnnn
     2941 gcgctggcgc cgctataggt cagactgtcg ttgacggcgt cgatccagcg cgtgcgcatc
     3001 cgcgtcgcgg gcgtcgtaca gggcgtcggg ttccgttaca tgacaaaaat ggtggctgac
     3061 cagatcggca ttaccggcat cgtcaggaac gaggccgacg gaagcgtcta tatcgaagct
     3121 gtcggacctg gcgataagat cgataccttc atcgcggcag tcaaacgcag tccctcgcca
     3181 agcggcaaag tagatagctg ctccatactt gaagacgcca acattgagaa ttatataagt
     3241 tttcgcgtaa ccaattgaat atatttcccc cttcaggtat agtggtaatg ttatcgaaaa
     3301 attgtaaagg aacgtgaacg atgaaactta aaaagaaatt attgctttcc gcagaaatga
     3361 tggcattatt agtggtcctg acaggctgca tgcaatatga tgagaataaa aatccaaccg
     3421 gcttcattta cgactatttg gtagtaccta caggcaattt gatcgttatg ctggcagaaa
     3481 tgcttaacgg caattatggg ctggccatca tcgccatcac aatcattgtc cgcttggcca
     3541 tcatgccctt gaactttagc caaatcaaaa aaacgatggt acaacaagag aaaatgaagt
     3601 atatcaaacc agaattggaa gatatccaat tccgtcaaaa aaatgcacaa tcgcctgaag
     3661 aaaaagcggc ggtttcgcag gaaatgatgg cgttgtacaa ggaaaacaac atcagcatga
     3721 caggcggcgt tggttgcttg ccgattctta tccagatgcc gatttttaca gctatgtatc
     3781 aggccgtcaa cctgacgccg gaaatctccc aaagcacatt ccttggcatc aaccttggtg
     3841 tcagcagtcc gttgttggca atcctggcgg gtgtggccta tgtgatccaa ggctacgttt
     3901 ccacaatcgg catgcctcag gaaaccaagt cacagatgaa atcaatgatg ctgatgaatc
     3961 cgatcatgat tctcatgttc tcatggtctt caccagcagg attggctctt tattggttag
     4021 ctggcggtat tttcgctgca gcccagactg cgcttcagaa tcacatgatc aaacctaaaa
     4081 tccagaagca agtcgaagaa gaaatgaaag atcgtccaat caagaagaat gtgaagatgg
     4141 ccaaaccagc gtcacagtca acctcaacgc aatctgttaa ggctctgccg gctaagaatc
     4201 aacaaactgg tggcaaaaaa ggtcgcaacg caggcaagca gaccaggtct taagcactgc
     4261 acaactataa aagaaaagca ggaagcggct cacacatgag ctggcttcct gcttttttgt
     4321 agtttatttt tggcacgagg caaatgtttt actatccaac gtctacagat cctcgtccgg
     4381 tacataattc ttcagaatcg gcaattggat tcggaaaaca gatccatggc ccaatacgct
     4441 ttcgacagtg atatcgcctt tatagccttc caataattct tttgcaatcg acaagcccaa
     4501 gccgttcccg cctttattcc gagacctggc tttatcgaca cggtaaaaac gcgaaaaaat
     4561 cttttgctga tcttcctggg acatcccctc gccaaaatct tggatagcaa tctggacgta
     4621 ggacatcgat tcggaaatcg aaagatggat ttcatgccgg tcggtcga
//
LOCUS       DAOMUJ010000097         4564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5300_length_4564_cov_2.213573, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000097 DAOMUJ010000000
VERSION     DAOMUJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_4564_cov_2.213573"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLT73_03630"
     CDS             <1..626
                     /locus_tag="PLT73_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM19506.1"
                     /translation="NQEKINLLLSELQTAYPGQEFYPICLDLTDEAEVTKLADHIFSL
                     QAIIFAHGRTEYGLLQDMTSMQMDDLWEMHVKMPILIIQTLYEKINRSGNGRIVFISS
                     IYGEVGSGLEVLYSTVKGAQIAFVKAYSKEVASMGVTVNAICPGAIDTQMNTHLQKDE
                     KEALLEEIPLGRMGKPDEISFWVSQLLKDDSRYMTGQAIYVSGGWLR"
     gene            713..1588
                     /locus_tag="PLT73_03635"
     CDS             713..1588
                     /locus_tag="PLT73_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM19507.1"
                     /translation="MVQIGEILKSARLSKGYTLDDLQQMTKIQKRYLIAIEEGDYEIM
                     PGNFYVRAFIKQYADTVGLDGDRLLGDHASVIPEIHGGVQVEDSGSFSPTRSETKRAS
                     KKTNFNYGGSVQSQLPTLLLAVAVIAIVLVIWKATLSDDGEQIQTEVASIISQTTLAS
                     QETGSSNTEQTDSAVVAEQTAQVTLVSSADGYAEYDITSLSLPSELKISAVAGGSSWI
                     SVSVNGIVQEPSGIVDSENPLTVALPLNTSEIEVVVGVMPATIIELDGAVVAMPADYQ
                     EIQTQTLSFTVKSVE"
     gene            1618..2196
                     /gene="pgsA"
                     /locus_tag="PLT73_03640"
     CDS             1618..2196
                     /gene="pgsA"
                     /locus_tag="PLT73_03640"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRM19508.1"
                     /translation="MNLPNKLTALRILMIPLFMIITLFPFDWGTLAVAQSQIAVNQLI
                     GAIIFAIASFTDWLDGYIARRDGLVTNFGKFADPLADKMLVATAMIVLVGQGLAPSWV
                     VSIIISRELAVTGLRLLLVKEGEVMAAAWPGKIKTATQMVAIILLFLDNFPFQAIGIP
                     VADIMLYLCLVFTIYSGVDYFIKNKHVFVGSM"
     gene            2351..>2858
                     /locus_tag="PLT73_03645"
     CDS             2351..>2858
                     /locus_tag="PLT73_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRM19509.1"
                     /translation="MNAEIISVGTELLMGQIVNSDAAFIAKQLTGLGIDSYFQTVVGD
                     NPGKIKEVIKIAESRSDLLIFTGGLGPTQDDLTKQTVADHLGEELVMDRTGLMHIRQW
                     FERSGRSMTQNNERQALVFRDGIVFPNPIGQALGTYIEKNEKGYLLLPGPPRELEQMF
                     RQSVTPFLS"
     assembly_gap    2859..2868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2869..3599
                     /locus_tag="PLT73_03650"
                     /pseudo
     CDS             2869..3599
                     /locus_tag="PLT73_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773215.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
     gene            3705..>4564
                     /gene="recA"
                     /locus_tag="PLT73_03655"
     CDS             3705..>4564
                     /gene="recA"
                     /locus_tag="PLT73_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRM19510.1"
                     /translation="MANLNDNRQKALDEALKKIERNFGKGSVMKLGEKVDTKISTVPS
                     GSLALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKKGGVAAFIDAEHALD
                     PKYAAALGVDIDELLLSQPDTGEQGLEIADALVSSGAVDIVVIDSVAALVPRAEIEGE
                     MGDSHMGLQARLMSQALRKLSGSINKTKTIAIFINQVREKIGIMFGNPETTPGGRALK
                     FYATIRLEVRRAEQIKSGTEIMGNRTKIKVVKNKVAPPFRTAEVDIMYGEGISQVGEI
                     IDMASEKDIV"
BASE COUNT         1266 a          935 c         1205 g         1148 t
ORIGIN      
        1 ctaatcaaga aaagatcaat ctgctgctgt ctgaacttca aactgcctat cctgggcagg
       61 aattttatcc gatttgcctg gatttgaccg atgaagctga agtaacaaag cttgctgacc
      121 atatattttc gctccaagcg attatttttg cacatggccg gaccgaatat ggcttgcttc
      181 aggatatgac ttccatgcaa atggatgact tgtgggaaat gcatgtcaag atgcctatcc
      241 tgatcatcca gacgctgtac gaaaaaatca atcgttccgg taacggccgc attgtcttca
      301 tcagctcaat ctatggagaa gtcggcagcg gtctggaagt gctctacagt accgtcaaag
      361 gggcgcagat tgcattcgtc aaagcataca gcaaagaagt cgcctcaatg ggagtaacag
      421 tcaatgccat ctgtccggga gcaatcgata cgcaaatgaa cacgcatctg caaaaagatg
      481 aaaaagaggc tctgcttgag gaaatccctt tggggaggat gggaaaaccg gatgaaatca
      541 gtttctgggt atcccaactt ctgaaggatg atagtcgcta catgactgga caggccattt
      601 atgtatcggg tggatggctc aggtaataaa gtattggaac tcctagtgaa ggaattttat
      661 ggtataatat ccagggtaac tcggggattt gcaagtgaag gcaggtgaag aaatggttca
      721 aattggtgaa atcctaaaaa gtgctagact ttccaaaggg tacacattgg atgatctaca
      781 gcaaatgact aaaatacaga agcggtatct gattgctatt gaagaaggcg attacgagat
      841 tatgccgggt aatttctatg tgcgggcctt catcaagcaa tatgcggaca ccgttggatt
      901 ggatggcgat cgcttgttgg gggaccatgc aagtgtgatt ccggaaatac atggcggtgt
      961 tcaagtggag gattccggct cattttctcc cactcgttcc gagactaaac gagcctcaaa
     1021 aaagacgaat tttaattatg gaggctccgt ccagagccag ttgccgacat tattgttggc
     1081 tgttgccgta atcgccatcg ttctagtgat ctggaaggct accttgagcg atgacggtga
     1141 gcagattcag accgaagtgg catccattat cagtcagaca accttggcat cgcaagaaac
     1201 gggttcaagc aacacagagc agacagacag tgccgttgtc gcagaacaaa cagcgcaggt
     1261 gaccctggta tcgtccgccg atggttatgc ggaatatgac atcaccagct tgtcgctgcc
     1321 ctcagagttg aaaatctcgg cggtggcagg aggcagttct tggatcagcg taagcgtaaa
     1381 tgggattgta caagagcctt cgggcatcgt ggacagtgaa aatccgttga cggttgcact
     1441 cccgttgaac acaagcgaga tagaagtggt cgtcggcgtg atgccggcaa caatcatcga
     1501 actggatggc gcagttgttg cgatgccagc cgattatcaa gagattcaga cccaaacctt
     1561 atcctttacc gtcaaaagtg tggaataagt cagggtacat taaaggagtt gcagaagttg
     1621 aatttaccta ataaattgac agcgttgcgg attttgatga ttccgctgtt catgatcatc
     1681 accttgtttc cattcgattg gggtacgctt gcggttgccc aatcacagat tgccgtaaat
     1741 caactgatag gggcaatcat ttttgccatc gccagtttta cggattggtt ggatggttac
     1801 atcgccagaa gggatgggtt ggtcaccaac ttcgggaaat ttgctgatcc attggcggac
     1861 aagatgcttg tcgccactgc catgattgtg cttgttggac aaggattggc tccttcatgg
     1921 gtcgtcagca tcatcatcag tcgggaattg gcggttacag gcttgcgtct cttgcttgtg
     1981 aaggaaggcg aagtcatggc agcagcctgg ccgggaaaaa tcaaaacagc aacgcaaatg
     2041 gtagccatca ttttgctgtt cttggataat ttcccatttc aggcaatcgg tatcccggtt
     2101 gcggatatca tgctgtatct ttgcttagtt ttcaccatct actcaggggt ggattatttc
     2161 atcaagaata agcatgtttt tgtcggctcc atgtagtttt tattaaatta agaagggcgg
     2221 ggttgcatca ggttgcgatc cgaacaaaag gaaacgtaat tcttttgtgc ccttcttttt
     2281 ctttatcttt tttatgcaat tgaatttttt gttgtattta agcgaatact atggatgtga
     2341 gggatcagat atgaatgcag aaatcatttc agtcggaacg gaattgttga tggggcaaat
     2401 agtgaattcg gatgcggcat tcatcgccaa gcaattgacc ggtttgggca tcgactcgta
     2461 tttccagaca gttgtcggag ataatccggg gaaaataaaa gaagtgatca aaattgctga
     2521 atcgcgaagc gacttgttga tcttcacagg aggactcggc cccactcaag acgacttgac
     2581 taaacagacg gtggcggacc atcttgggga agagcttgtc atggatagaa ctggattgat
     2641 gcatatccgg caatggtttg aacgctctgg aagaagcatg acgcagaaca atgagcgtca
     2701 agcattggta ttcagggacg gtatagtctt cccaaaccca atcggacagg cgctcggtac
     2761 ctatattgaa aaaaatgaga agggttacct gctgttgccg ggcccgccaa gagaacttga
     2821 gcaaatgttc cggcaatccg tgacgccatt cctgtccgnn nnnnnnnnaa gtcatcttat
     2881 cgaagaccct ccgctttttt ggaatcggtg aatctacctt gacaacgcta ttggatgaaa
     2941 tgatccgcaa tcagaccaat cctacgatag caccctatgc cgggaaatac gaagtgacct
     3001 tgagaatcac cgcaaatgcc gcaacagagg cggtatgcga aaagttagtg gatgaaacaa
     3061 aagcggaaat attggcgatc gtcggtgaac accattacgg tgacggcgac gaatcaagct
     3121 tggctgaagt ggtaggaaag aaactcttgg agagcaaacg ctccatcagt gcagcagaaa
     3181 gtttgaccgg agggcttttt caatcggaac ttgtcaaagt tcctggcata agcgaggtct
     3241 tcgcgggagg catcgtatct tatcaagctg aggcgaaaca acgggttctt ggcgttccgg
     3301 agcatgtcat agaaacttat ggaatggtga gcccggagtg cgcggttgcg atggcggaat
     3361 gcgcgcgcga attgtttgac accgatctgg ccatttcgtt cacaggcgta gccggacctg
     3421 atcaaatgga gaataagccg gccgggacgg tgtggattgc cttaagcaaa aaaaatggac
     3481 cgaccgccgt ccaaggtttt cgattttccc gcgatcgtct cgggaacagg gagcaaaccg
     3541 tcatgcaggg gtttgatatg atcaggcggc atttgatgga ttctgattta gaaagataaa
     3601 aaaacgaaca tctgttcact ttattcttgc ttttttacac agaaaaggat agtattacgt
     3661 tgtggtgtta atgcatgcaa atgaaagctt ggaggaaaca attgatggct aatttaaatg
     3721 ataatagaca aaaagccttg gatgaggctc tgaaaaagat agaaagaaac tttgggaaag
     3781 gttctgtcat gaaacttggg gaaaaagtgg acacaaaaat ttccactgta ccaagtggct
     3841 cattggcgct tgatgttgcg ttgggtgtag gcggatatcc gcgaggccgt atcatcgaag
     3901 tgtacggtcc tgaatcatca ggtaagacaa cagtcgccct ccacgcgatt gctgaggtac
     3961 aaaaaaaggg cggcgttgct gcatttattg atgctgagca cgctttggat ccaaaatatg
     4021 ctgcggcttt gggtgtggat attgacgaat tgctgctttc ccaaccggac acaggtgaac
     4081 aaggtctgga aatcgctgat gcattggttt catcaggtgc tgtcgacatt gtggtcatcg
     4141 attcggttgc cgcattggtt ccgcgtgctg aaattgaagg cgaaatggga gactcccata
     4201 tgggtctgca agcacgtctg atgtcccagg ctttacggaa actgtccgga tcgatcaaca
     4261 aaacaaaaac gattgccatc ttcatcaatc aggtccgcga gaaaatcggt atcatgttcg
     4321 gtaatccgga aacgactccc gggggtagag cgcttaaatt ttatgctact atccgactgg
     4381 aagtcagaag agcggaacag atcaagagcg gaacagagat catgggtaac cgcacaaaaa
     4441 ttaaggtagt caaaaataaa gttgcgccgc cattcagaac tgctgaggtg gacatcatgt
     4501 acggcgaagg catttctcaa gtcggcgaaa tcatcgatat ggcttcagag aaagatatcg
     4561 tcaa
//
LOCUS       DAOMUJ010000098         4559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5307_length_4559_cov_2.283304, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000098 DAOMUJ010000000
VERSION     DAOMUJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5307_length_4559_cov_2.283304"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..922
                     /locus_tag="PLT73_03660"
     CDS             71..922
                     /locus_tag="PLT73_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19511.1"
                     /translation="MNKRFGLLLVVCVGVFAACQNESETEDSLVSEQSSVSAEAEKSV
                     TKTILFSSGVEDGITRGDKYNYFALEGNAEGYDQVTTIIEGTAVDYRETNNVWEFDYP
                     YQGPSIETEITFTTDTSVIYGDTGIKLADLAPESYVTITFIPNENPTITPPAFTVNEG
                     EAQNFLSDDSGINELVTVTEIREIDALEELNPLGEKLLEVEVQYVNNGSVDTYIAPNY
                     FSALDAEGKILPLRYTHFWLHTVAPGESFTDKIYFDITSEGPYAIQFFDGAWIDSEEV
                     GETVKDI"
     gene            complement(1049..1483)
                     /locus_tag="PLT73_03665"
     CDS             complement(1049..1483)
                     /locus_tag="PLT73_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM19512.1"
                     /translation="MLAEYKNILVPVDGSGQSEDSFKKAVAIAKRNNAALHLIYVKDT
                     RNVPLSPEYESRLTEAYKDMEYEFLDEMMTFALNEGIEVKKSVTNGNPMTLIAEAFPK
                     EYNIDLIVIGATGKGAITRAFVGSVSNYVVRHAPCDVLVVRT"
     gene            1630..2196
                     /locus_tag="PLT73_03670"
     CDS             1630..2196
                     /locus_tag="PLT73_03670"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRM19513.1"
                     /translation="MNLRNKRGLKTDTNHEQEIRYCRQTKVIQTHHVFPFDSNYHGTL
                     FGGKLMSMIDDCAAISGERFGRTTNVTASVDTLNFIKPLPTGHSVCIDTFVSGAGKTS
                     MEIFTKVTGENLRTGERYLAATCFLTFVALPDGNGQKVSLPKIVPETVEEKFINSGYE
                     ERRKKRRADLDYQKQLHEHLTIEIPWAD"
     gene            2285..3916
                     /locus_tag="PLT73_03675"
     CDS             2285..3916
                     /locus_tag="PLT73_03675"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRM19514.1"
                     /translation="MSKTNGKDLLGQPFLNKGTAFTLEERATLGLEGLLPTAVRTLEE
                     QGEIVYAQLGQLTDAYSKQKHLMNIYAQNRVLFYHVIGKHVTELLPVIYTPTIADTVM
                     NYSRDYQIPQDAVYLDVNRPEAIRTALLNGCKGMDEEIKMMVITDGEGVLGIGDWGIQ
                     GIAISIGKLAVYTVASGLNPQQVLPIVIDAGTNNEQLLEDPFYLGNKHKRVTGEAYYP
                     FIEKFVEEASALFPDVLFHWEDFGRDNAANILEQYRDKICTFNDDIQGTGVMMAAAMD
                     SVVRITDKPITEHKILVFGGGTAGVGVSDQILMEMVLQGADSQEARKQFYMVDRYGLV
                     TDDMADLTPGQQKYARAAAEFSAPLNDLAEIIEAVKPTVLIGTSGQAGAFTQAVVEKM
                     AAYNERPAIMPISNPTRLCEAKASDVIAWSEGRALVVTGSPSDPIAYNGVDYKIGQAN
                     NALLYPGLGFGIVIAKAKTVTDRMLSAAAHGITSLQNLDEAGAAILPPVSQLREASKL
                     VAAAVIQAAIEDGVNGVEITDPMEAVEAAIWKAEY"
     gene            3968..>4559
                     /locus_tag="PLT73_03680"
     CDS             3968..>4559
                     /locus_tag="PLT73_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM19515.1"
                     /translation="MEYRIEHDSLGEVKVAADKLWGAQTQRSLENFKIGIEKIPMEVI
                     YALVEIKRAAAASNHEVGLLDETVTKGILLAADEVLEGKWDDQFPLSVWQTGSGTQSN
                     MNVNEVLANRGNQLVDGGIHPNDHVNKGQSSNDTFPAAMHVAGVLYIKNKLYPAIEQL
                     IGTLTRLEQENAAIVKLGRTHLQDATPLTLGQEISAW"
BASE COUNT         1294 a          997 c         1194 g         1074 t
ORIGIN      
        1 atttgctaaa atgggggtaa gaatcgaaag atggaaacct gttgtgcgga ttgcgggagg
       61 gggaattcct atgaataaaa ggtttggatt gctgcttgtt gtctgtgtcg gtgtttttgc
      121 tgcctgtcaa aatgaatctg aaaccgaaga ttctttggtg agcgaacaat cttcggtttc
      181 tgccgaagct gagaaaagtg taactaaaac gatccttttt tcaagtggtg ttgaggatgg
      241 aatcaccaga ggcgataaat ataactattt tgcgttagaa ggcaatgctg aaggttatga
      301 tcaagtgacc accatcatcg aagggacggc tgtcgactat cgcgagacga acaacgtatg
      361 ggaattcgat tatccttatc aaggacccag tattgaaaca gaaatcacct tcacgacgga
      421 tacatccgtc atatatggcg atactgggat taaactggca gaccttgcgc ctgaaagtta
      481 tgtcaccata actttcatac ctaatgagaa cccaacgata actccgcctg cttttacggt
      541 caatgagggt gaagcgcaaa actttttgag cgacgattcc gggataaatg agttggtgac
      601 ggtaacggaa atccgggaaa tagatgcgct ggaggaactc aacccgcttg gggaaaaact
      661 attggaagta gaagtacaat acgtcaacaa tggcagtgtc gatacctaca tcgctcccaa
      721 ctatttttcg gcgcttgatg ccgaggggaa aattctccct ttgcgctaca cccatttctg
      781 gttgcatact gtcgcacccg gggagtcctt tacagataag atttattttg acattaccag
      841 tgaagggcct tacgcgatcc aattttttga tggcgcttgg atcgacagcg aggaagttgg
      901 cgagacagta aaggatatct agctgactga aggataccaa aaactacggt aaaacgtcct
      961 atacaaggaa acagccttgt gcagcttaag ctgtacaggg ctgttcttac gttttctgag
     1021 gtacggctgc aaaaatagtg cgctactttt aggtacggac aaccaaaaca tcacacggtg
     1081 catgacggac gacatagtta gaaaccgatc caacaaaagc cctggtgata gcacctttgc
     1141 cggttgcacc gatgacgata agatcgatgt tgtattcttt cggaaatgct tcagcgatga
     1201 gcgtcattgg attgccgttg gttacagact tcttgacttc aatcccttca tttagggcga
     1261 atgtcatcat ttcatcgagg aattcatatt ccatgtcctt gtatgcttcc gtcaatctgg
     1321 attcatattc agggctaaga ggaacatttc ttgtgtcctt aacgtagata aggtgcaacg
     1381 cggcgttatt acgtttggca atggcaacag cttttttgaa cgaatcttct gactgacctg
     1441 aaccgtcaac gggaaccaaa atatttttgt attctgctaa catttctacc cgctcctttt
     1501 ttgaatttgt tcagaaaacg cttcctatac tcattttaca acaaccctgt ttgaaaagga
     1561 agccgggaag cggtgacaaa aattagaatt atttggcaga actgttaatc agaaatgaag
     1621 catgctaaaa tgaatttacg aaacaagaga ggattgaaga cggatacgaa ccatgaacag
     1681 gagatcagat attgcaggca gacgaaggtg attcagacac atcacgtctt tccgttcgat
     1741 tcaaattacc acggtacttt gttcggaggc aaactgatga gcatgatcga tgattgtgcg
     1801 gcaatttccg gagagcgttt cggtcgtacg accaatgtga ccgcatcggt ggacacattg
     1861 aacttcatca agccacttcc tactgggcac tcggtctgca tcgatacgtt tgtttcggga
     1921 gctgggaaga catcaatgga aatcttcaca aaagttaccg gagaaaatct tcggacgggt
     1981 gaacggtatt tggcagcgac ctgctttttg acctttgtgg cgcttcctga tgggaatggg
     2041 caaaaggttt ctcttccgaa aatcgtaccg gaaacggtcg aggaaaaatt catcaacagc
     2101 ggatatgaag aaagacggaa aaagcgaaga gccgatctgg actaccaaaa acagctacat
     2161 gaacatctca cgatagaaat accttgggcg gattagcact tgtagacaaa ataagcgcgg
     2221 caagccttga tttgaaaagc atggaaagag atacatacaa atgatggagc aggagtgtgt
     2281 caacgtgtcg aaaactaacg gaaaagattt attgggtcag ccatttttga ataaagggac
     2341 cgcattcact ttggaagaac gagcaacatt agggctggaa ggtttattgc cgaccgcagt
     2401 gcgtaccttg gaagaacaag gggagattgt ctatgcgcaa ctgggccaac tgactgatgc
     2461 ttattccaaa cagaagcatc tgatgaatat ctacgcccag aaccgcgtgc ttttttacca
     2521 tgtcatcggg aaacatgtga cggagttgtt gccggtaatc tatacgccga ccatcgccga
     2581 cacagtcatg aattattcgc gggattacca aattccccag gatgcggtct acttggatgt
     2641 gaatcgtccg gaagcgatcc gcaccgcttt gctgaacggc tgcaaaggca tggatgaaga
     2701 aatcaaaatg atggtcatca cagatggcga aggggtgctg ggcatcggcg attggggcat
     2761 ccaaggcatc gcgatttcaa tcggaaaact ggctgtatac accgtcgctt cgggattgaa
     2821 tccgcaacag gtcttgccga tcgtgattga tgcgggcaca aacaacgagc agctgctgga
     2881 ggatccgttc tatctcggca ataagcacaa acgtgtgaca ggagaggcgt attacccatt
     2941 catcgaaaag ttcgttgaag aggcatcagc gctgttcccg gatgtgttgt tccactggga
     3001 agacttcggc cgcgacaatg ccgccaatat actggagcag taccgcgata agatctgtac
     3061 gttcaatgat gacatccagg ggaccggcgt gatgatggcg gctgcgatgg attcagtggt
     3121 ccggatcacc gataagccga tcacggaaca caaaatcctt gttttcggag gcgggactgc
     3181 cggtgtcggt gtatccgacc aaatcctcat ggagatggtg ctgcaaggtg ccgacagcca
     3241 ggaagcccgc aaacagtttt acatggtcga ccgctatggg ctggtcactg atgacatggc
     3301 ggatcttacg ccgggtcagc aaaaatatgc gcgcgctgcg gctgagttct ccgcaccatt
     3361 gaacgatttg gctgaaatca tcgaagcggt caaaccgacc gtgctgattg gtacatcagg
     3421 ccaagccggt gcctttacgc aagcggtcgt cgaaaagatg gcagcataca atgaacgtcc
     3481 ggccatcatg ccgatctcga atccgaccag attatgcgaa gcaaaggctt cggatgtcat
     3541 tgcttggtcc gaggggagag cgttggtcgt taccggcagc ccatccgatc cgatcgcata
     3601 caatggcgtg gactataaaa tcggccaggc gaacaatgcc ttattgtacc caggtttagg
     3661 cttcgggatc gtcatcgcaa aagccaagac agtgacggac agaatgctgt ctgctgccgc
     3721 gcacggcatc acttccctac agaacttgga tgaagcggga gcagcgattt tgccgccggt
     3781 ttcccagttg cgcgaagcct cgaaattggt tgcggcagcc gtcatccaag cagctatcga
     3841 agatggagta aatggtgttg aaataacgga tccgatggaa gcagtcgaag ctgccatctg
     3901 gaaagcggaa tattaggaca aaaagcacgt ccaaaaacgg aagagccgac aggaaggaac
     3961 gggatgaatg gaatatagga ttgaacatga ctcattaggg gaagtaaaag ttgcagcgga
     4021 taaattatgg ggtgcacaga cgcaacgcag tctggaaaat ttcaagatcg gaatcgaaaa
     4081 aattccgatg gaagtcatct atgcattggt cgaaatcaag cgtgctgccg cggcatcaaa
     4141 ccatgaagtc ggcttattgg atgagaccgt tacaaaaggc atccttttgg cggctgatga
     4201 agtgcttgaa ggcaaatggg acgatcaatt tccgttatct gtatggcaga ccggaagcgg
     4261 cacgcaatcc aatatgaatg tgaacgaagt gctggcgaac cgaggcaacc agctagttga
     4321 cggcggtatc caccccaacg accatgtgaa taaaggtcag agctccaatg acacattccc
     4381 ggctgccatg catgttgccg gtgtgctgta catcaaaaat aaactgtatc cggcgattga
     4441 gcaattgatc ggcacgctga cccgattgga acaggaaaac gcggcgatcg tcaaattggg
     4501 ccgtacccat ctgcaggatg cgacaccatt gacattaggc caggaaatca gcgcatggc
//
LOCUS       DAOMUJ010000099         4537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5352_length_4537_cov_2.686078, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000099 DAOMUJ010000000
VERSION     DAOMUJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_4537_cov_2.686078"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLT73_03685"
     CDS             complement(<1..337)
                     /locus_tag="PLT73_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM19516.1"
                     /translation="MGKSLVLAEKPSVARDIARVLQCEKQGQGYLEGKDYIVTWALGH
                     LVTLADPEAYDVKYKNWNLADLPMLPQDLKLTVIKQSGKQFNAVKAQLVRNDVSDIII
                     ATDAGREGEL"
     gene            complement(458..1009)
                     /locus_tag="PLT73_03690"
     CDS             complement(458..1009)
                     /locus_tag="PLT73_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM19517.1"
                     /translation="MEPDRNVKKTVNFGVRRLALLSILTALCYVGRMMFQFIPNVQPV
                     TAVLIILTLTLGMTDGFIVAILSIVLTNFLLGMGPWTIAQIASFGVLIVVTGLVMKPF
                     YSTSQKRGLRIVFALFAFFGGLLYGFVISVISVKLMGINSFWAYYIAGIPFDLMHGLG
                     NASFYLILEPILAPLLLAQKKKT"
     gene            complement(988..1410)
                     /locus_tag="PLT73_03695"
     CDS             complement(988..1410)
                     /locus_tag="PLT73_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRM19518.1"
                     /translation="MKKLLLALSLSLVLFGCAPADTVDSASVAESSSVAISSEVAETV
                     SVSISLTDGGTVIAGSEKTIEVEAGANLLAVMKENYTIEEADGFITAIDGQTQVEQTA
                     DTKAKYWLFDVNGEPSMVGAADVALQEGDVIVWNLTEM"
     regulatory      complement(1498..1654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            1945..4077
                     /locus_tag="PLT73_03700"
     CDS             1945..4077
                     /locus_tag="PLT73_03700"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRM19519.1"
                     /translation="MDEHKHTQHAHTDHTLEAPKTSDPHAGHDHSGHHMPPPEPKQTA
                     SNGHEQHEKHMAHGSTGHADHHEQMVADYRKRFWIVLLLTIPVTVLSPMIMMLFGYHF
                     EFPGANFVVFGLSTVIFFYGGKPFLEGAWEEWKNRSLGMMMLISLAIVSAYIYSSFTA
                     FFIEGSDFYFELATLILIMLLGHWIEMKSQMGASKALEELIKLMPKVAHRLDAAGNIE
                     EVSIEALVPGERILVKPGEKIPLDGKIYEGLSTVDESMLTGESVPVEKNPGMQAIGGS
                     INGSGVLKLTVEKVGNETYLAQVINLVQAAQQQKSKSQGLADKAAGWLFYIAVTAGLL
                     TFLYWIFAANLAFALERMVTVLVIACPHALGLAVPLVNAVSTSIAARNGLLIRNRTQF
                     EEARKVDRIVFDKTGTLTEGNFGVTDILPAAGVSEKELLTLAYSAESQSDHPIAKGIV
                     RKGEAEGVTLLPATDYQNLTGQGLQVTVDGMQVAVVSPGTMDERGIAYDKETFASLAQ
                     QGKTVVFVLKEGSLQGMIANADIIRSSSQEVVAALKEQGIEAIMLTGDNSRAAQYVGD
                     QLGLDQVFAEVLPAEKSKHIAALKSGNKKVAMVGDGVNDAPALAEADLGIAIGAGTDV
                     AIETADVILVDSNPKDVLTIITLSKATYRKMVENLIWAVGYNVIAIPLAAGVLANQGF
                     LITPAIGAAVMSISTIICAVNARLLKIT"
     assembly_gap    4162..4171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4222..4416
                     /locus_tag="PLT73_03705"
     CDS             4222..4416
                     /locus_tag="PLT73_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRM19520.1"
                     /translation="MGGMMFMWIFWIALIILVIYLATRLLNDRSGKVSSDESALDILK
                     KELAKGNITEEEFDRKKKLL"
BASE COUNT         1152 a         1205 c         1099 g         1071 t
ORIGIN      
        1 ccagttcgcc ctcgcggccg gcatccgtcg cgatgatgat gtcggaaacg tcattgcgca
       61 ccagctgggc cttgacggca ttgaactgct tgccggactg cttgatgaca gtcagcttca
      121 gatcctgcgg aagcatcggc agatcagcca aattccaatt tttatatttc acatcatagg
      181 cttccggatc ggccagcgtc acaagatgcc ccaaggccca agtgacgata tagtctttcc
      241 cttccaggta cccttgaccc tgtttctcgc actgcagcac gcgggcgata tcgcgcgcca
      301 ccgatggttt ttcagctaat actagactct ttcccataat ttatatttgc tcccttatct
      361 ctcgattatc gtgtcctact atatcacaaa tggcgccaaa acagaaaaaa ctatagcgga
      421 tacccatgcc gggagtccgt tacagtcttc ttaatttcta tgtttttttc ttttgcgcca
      481 gcagtaaggg cgccaaaatc ggctccagta tcagatagaa actggcattc cccaggccgt
      541 gcatgagatc gaaaggaatc ccggcgatgt aataagccca aaaactgttg atacccatca
      601 acttcaccga aatgacggag atgacgaatc cgtaaagcag tcccccgaaa aaggcgaaca
      661 gcgcaaaaac aattctgagg cccctttttt ggctcgtgct gtagaacggc ttcatgacca
      721 atccagttac cacgatcaga acgccgaaac tggcgatctg cgcaatcgtc cacggcccca
      781 tccccagcag aaagttggtc aaaacaatcg acaggatggc cacaatgaag ccgtccgtca
      841 tgcccagcgt cagcgtcagg atgatcagca cagccgtcac cggttggaca ttcgggatga
      901 actggaacat catcctccca acatagcaca aagccgtcaa aatcgacagc aacgccagtc
      961 gtctcacacc gaagttcaca gtcttcttta catttctgtc aggttccaca cgattacgtc
     1021 tccttcttgg agcgccacat cagctgctcc gaccatcgaa ggctcaccgt tcacatcaaa
     1081 caaccaatat ttcgctttcg tatcggcagt ctgttcgact tgtgtttgtc catcgatggc
     1141 tgtgatgaat ccgtccgctt cttcgattgt gtagttttct ttcatgacag ccaacaaatt
     1201 ggcgcctgct tcaacctcaa tcgttttttc agaacccgcg atgacagtac cgccatccgt
     1261 caaagaaatc gatacgctga ctgtttctgc aacttcggag ctgattgcaa ccgatgaaga
     1321 ttccgcgact gaagccgaat ctaccgtgtc cgcaggagcg cacccgaata ataccaaact
     1381 caatgataag gccaacaata attttttcat tttcccactc ctaatctgta ttcgacaggg
     1441 gttgcatcgc caacggccgg aatcatgcaa aaaaagccta tacgccatcg catagactag
     1501 taacgggacc gttttacctc gaaacggttc gcttgcaaaa aatagcagtt tggaatgaag
     1561 acctgactta gacgttccgg gacgtcatca cagtggcggg accgcgttgg attttcacca
     1621 aacttccaaa ttccaagtgt ctttgaatgg ttttcctatc cctttcattt tacccaaagc
     1681 agaaaaaaat acaagaccag ttgcgctttt cggtgcgccc tggtcctgca attgataaga
     1741 agtctgtcaa gtcaatcttt ggttcccggt tacgcctgca agcttccgcc gttggttgcg
     1801 atgactaaat cggtgcagac ccaagcgggg aggcgaaatc attaccacta cagcgctgaa
     1861 ttctgctgtg ataagcatga gaagctgcag tcatttttat aaaccttatt gtttggacgt
     1921 tttgaagcaa ggaggaatac tcagatggat gaacacaaac atacgcagca cgcgcacaca
     1981 gaccacactc tggaagcccc aaagaccagc gatccgcacg ccgggcacga ccattccggt
     2041 catcatatgc ccccacccga accgaaacag actgcttcca atggtcacga acaacatgaa
     2101 aagcacatgg cacacggttc gactgggcat gccgaccacc acgagcagat ggtggccgat
     2161 taccggaaac gtttttggat cgtcttgctg ctgaccattc ccgtcaccgt gctatccccc
     2221 atgatcatga tgctcttcgg ttaccatttc gaatttccgg gagcaaattt tgtcgtcttt
     2281 gggttatcca ccgtcatttt cttctacggc ggcaaaccgt ttctggaggg cgcttgggag
     2341 gaatggaaga accggtcgct cggtatgatg atgctgattt ccttggcgat cgtttcggcc
     2401 tatatctaca gcagcttcac ggccttcttc atagaaggat cggactttta tttcgaactg
     2461 gcaaccttga tcctgatcat gttgctgggt cactggatcg aaatgaaatc ccagatgggg
     2521 gcatccaaag cgctggaaga actcatcaaa ctgatgccta aagttgccca tcgtttggat
     2581 gcggccggca atattgaaga agtgtcgatc gaagcccttg ttccgggtga gcgcattctc
     2641 gtcaagcccg gtgaaaagat ccctttggac ggaaaaatct acgaaggcct ctcaacggtt
     2701 gatgaatcga tgctgacggg cgaatctgtg cctgtcgaaa aaaatccggg catgcaggcc
     2761 atcggcggct ccatcaacgg gtcaggcgtc ctcaagctga ccgtggaaaa agtcgggaat
     2821 gaaacctact tggcccaagt gatcaatttg gtccaggcgg cccagcaaca aaaatccaag
     2881 tcgcagggat tggccgacaa agcggccggt tggctgttct atatcgccgt cacagccgga
     2941 ctcctgactt tcctttattg gatttttgca gcgaacttgg ccttcgcttt ggagcggatg
     3001 gtgacggtac tcgtcatcgc ttgtccccat gcactcggac tggctgtccc tttggtgaat
     3061 gcggtgtcca cttcaatcgc agctcgaaac ggcttgctga tccgcaaccg gacgcaattc
     3121 gaagaggcgc gcaaagtgga tcggatcgtc ttcgacaaga ccgggaccct gacggaaggc
     3181 aacttcggcg taaccgacat ccttcccgct gccggggtct ccgagaagga attactgacg
     3241 ctggcctatt ctgccgaaag ccaatctgat cacccgatcg caaaaggaat cgtccgcaaa
     3301 ggagaagcag aaggcgtcac ccttttgccg gcaaccgatt atcaaaacct taccggccaa
     3361 ggtctgcagg tcactgtgga cggcatgcag gtcgctgtcg tcagccccgg caccatggac
     3421 gagcgcggca ttgcatacga caaggagaca ttcgccagtt tggcccagca aggaaaaacc
     3481 gttgtcttcg tcctgaagga agggagcctg caggggatga tcgccaacgc tgatatcatc
     3541 cgttcgtcat cgcaagaagt cgtggctgcc ttgaaagagc aaggcatcga agctatcatg
     3601 ctgactggcg acaatagccg cgcagcgcaa tacgtgggcg atcagttagg gctggatcaa
     3661 gtatttgcgg aggtgctgcc tgccgagaaa tcgaaacaca tcgccgcact gaaatccgga
     3721 aacaaaaaag tcgcgatggt cggggatggc gtcaatgatg cgcccgctct ggcggaagct
     3781 gatcttggta ttgccatcgg cgccggaacg gatgtcgcca tcgaaacagc cgacgtcatt
     3841 ctggtggaca gcaatccgaa agacgtattg accatcatca ccctttcgaa agccacttac
     3901 cgcaagatgg tcgagaatct gatttgggct gtcggctata acgttatcgc catcccgttg
     3961 gctgcaggtg tattggccaa tcagggcttc cttatcaccc cagccatcgg ggcagccgtc
     4021 atgtcgatca gcacaatcat ctgcgcggtc aacgcacggc ttttgaagat aacctgagca
     4081 tgtatttcaa ataatcaccg caacagccag aaaaactaca tccaatctac acaatatgac
     4141 tgtatactgc atatatagag annnnnnnnn nggggccttt gcatgtttgg cagatgctta
     4201 caatattttg gaggttttgg catgggcggc atgatgttta tgtggatttt ctggattgct
     4261 ttgatcatac tcgtcattta cttggcaacc aggcttttga acgatagatc aggcaaagtt
     4321 tcatcggacg aatccgcttt ggatatcctg aagaaggaat tggctaaagg gaacatcacc
     4381 gaagaagagt tcgatcgcaa gaagaaactt ctgtgatcct gaaagaaaca caaaagacct
     4441 ggataccaat caaaatgatt ggtatccggg tctttttcgt tcatgttctg atgtgttcca
     4501 atggaagtgt aaccgtgaac gcggctccct tgccgat
//
LOCUS       DAOMUJ010000100         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5440_length_4492_cov_2.317332, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000100 DAOMUJ010000000
VERSION     DAOMUJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_4492_cov_2.317332"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLT73_03710"
     CDS             <1..309
                     /locus_tag="PLT73_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRM19521.1"
                     /translation="DLLIYLDGSFETILDHIRKRGREFEQIEDDSELLSYYELLFKNY
                     EQWYQEYDQSPKIKINIDTFDIVNNSGDEEKVMSIIENALLEVRGEEVELTCAGSNIV
                     "
     gene            480..1073
                     /locus_tag="PLT73_03715"
     CDS             480..1073
                     /locus_tag="PLT73_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRM19522.1"
                     /translation="MMAADKAEQAVNVEQWQAINERDGHFDGQFYYADRNTQLYCKPS
                     CPTQIPKFNHVCIFSSVEAAEAHGYSPCRKCRPNGKAVTDLEWAGQAERFIHNYYPNP
                     LSVNRIAEACHGPSSELQQIFIRIYGVSLLDYLDRVRMDQARYLLIHSRLFIKQIAEL
                     VGMPAAADFSRKFKEKEGVAPTEYRKRARKLDTDQHS"
     gene            1237..1713
                     /locus_tag="PLT73_03720"
     CDS             1237..1713
                     /locus_tag="PLT73_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HRM19523.1"
                     /translation="MTELAQKKITDAEWEVMRVVWTNGKATSREIISVLQEKMDWKQA
                     TIKTLIGRLVDKGLLATEPIGNKFIYSANVSEQESVGGATEDLLAHVCTKKVGRTIAE
                     LIDRATLSHADVAILEKAIAIKKRTAVDEVKCMCTPGQCNCKQTGCVHKEESPDGK"
     gene            1703..4168
                     /locus_tag="PLT73_03725"
     CDS             1703..4168
                     /locus_tag="PLT73_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM19524.1"
                     /translation="MENKSYDIEGMSCASCSQAVEKAAAKVAGVKEATVNLATEKLTV
                     TVDESQFSENLLKNAVDAAGYVLVANKKEKTFLIEGMSCASCSQTVEKVTRAVAGVSD
                     ASVNLATEKMFVSYDPTMLHPGDIERVVAEAGYKAIEESSTIEESDQNQAKKEQHIKN
                     MWRRFWLSAVFTIPLLYLAMGHMLGLPLPMSLHPDMHPVTFALTQLVLTIPVMLLGTK
                     FFTVGFKSLFKGHPNMDSLVALGTSAAFLYSLYGTVAAIGGNNELVMNLYYESAAVIL
                     TLITLGKYFEAVSKGKTSEAIKKLMGLAPKTARVIRDGNEQEIKLDAVMVGDILVVRP
                     GEKMAVDGKVTEGLTSVDESMLTGESMPVEKKAGDAIIGGSINKNGTIQYRAEKVGKD
                     TALAQIIKLVEDAQGSKAPIAKLADTISGYFVPIVIVLAVLAGLAWYFMGQESWIFAL
                     TITISVLVIACPCALGLATPTAIMVGTGKGAENGVLIKSGTALEVTHKIQTIVFDKTG
                     TITEGKPKVTDVLVREGLDPNELLLLAASAEKGSEHPLGEAIVRDAEEKQLSFLKPSA
                     FNALPGFGIAVTIDGKELLLGNEKLMLNHAIGLGSFAETSHTLAEQGKTPMYIAMDGE
                     LAGIIAVADTVKASSATAIKKLRDMGIEIAMITGDNKRTAQAIANQVGIDRVLSEVLP
                     EDKAEEVKKLQQGGRKVAMVGDGVNDAPALAQADIGIAIGSGTDVAIESADIVLMKSD
                     LMAVATAVELSKATIKNIKENLFWAFAYNVLGIPVAMGILYVFGGPLLNPMIAGAAMS
                     FSSVSVVLNALRLKGFKPSVSKR"
     gene            4203..4412
                     /locus_tag="PLT73_03730"
     CDS             4203..4412
                     /locus_tag="PLT73_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRM19525.1"
                     /translation="MKKELLLEGMKCDGCVATVKEKFSAIAGVEEVIVDLEEKTATVK
                     MNQDISETTFNEALSGTKFKVTGSK"
BASE COUNT         1285 a          999 c         1215 g          993 t
ORIGIN      
        1 gatttgctga tctatctgga tggttcattc gagacgattt tggatcacat ccgcaagcgc
       61 ggccgtgaat ttgagcagat cgaggacgat tccgagttat tatcctacta tgaattactt
      121 ttcaaaaatt atgagcaatg gtatcaggaa tacgatcaaa gtcccaaaat caaaatcaac
      181 atcgacacat tcgatatcgt caacaacagc ggcgatgaag agaaggtcat gagcatcatc
      241 gaaaatgctc tcttggaagt ccgcggcgaa gaggtcgaat tgacttgtgc cggaagcaat
      301 atcgtataag tgacagatcc gttcaggtcg tcatcaaaca gcagcagtgc cattcggtgc
      361 gactgctgtt tttttggtga gtaggcctaa atttttcgcg acggactgaa gcatatccgt
      421 ggtttatata ctataattag gaaacagaca taaatgacag ttgaagagga tggaaccaga
      481 tgatggcggc ggataaggca gaacaagcgg tgaatgtgga acaatggcag gcaatcaatg
      541 aacgggatgg gcattttgat ggacaattct attacgcgga caggaataca caactgtact
      601 gcaagccgtc ctgccctacc cagattccga aattcaacca tgtctgcatc ttttcctcgg
      661 tggaggctgc cgaggcgcac ggctacagtc cttgccgcaa atgcaggccg aacgggaagg
      721 ccgttacgga tctggaatgg gccggtcaag ctgaacgctt catccacaac tattatccaa
      781 acccgttgag cgtgaatcgg attgccgaag cttgtcatgg gccttcgtcg gaactgcagc
      841 agatttttat ccggatatac ggcgtttcgt tgctggacta tctcgaccgt gtccggatgg
      901 atcaagcacg ctacttgctg attcactcga gactcttcat caagcaaata gctgagctgg
      961 ttgggatgcc cgccgcagcg gatttttccc gcaaattcaa ggaaaaggaa ggcgtggctc
     1021 cgacagaata ccgtaaacgt gcgcgtaagc ttgacacaga ccaacactcc tgaagagtta
     1081 cagcacaaat aaaaaaatgt agagctaaac agcaggctga ccgatcaatg gtaaccagct
     1141 gtttttttat tttccgatga aatcatttga cacaaatcgt ttacaagtgt aaacttgaat
     1201 tcataagtaa gaataaacaa cagaagggtg aaaaaaatga ccgaactggc acagaagaaa
     1261 atcaccgacg ctgaatggga agtcatgcgc gttgtctgga cgaacggaaa agcaaccagc
     1321 cgggaaatca tttcggttct gcaggaaaaa atggattgga aacaagccac catcaaaacg
     1381 ttgatcggaa gactcgtcga caaaggcttg ttggcaacgg aacctatcgg aaataaattc
     1441 atctacagcg caaacgtatc cgagcaggaa agcgtcggcg gtgctaccga agaccttttg
     1501 gcacacgtct gcacgaaaaa agtcggccgc acgatagcgg aactgattga tagagcaaca
     1561 ttgagtcacg cagatgtggc gatattggaa aaggcgatcg ccatcaaaaa acggacggca
     1621 gtcgatgaag taaagtgtat gtgcacgcca ggacaatgca actgcaaaca aaccggttgc
     1681 gtacacaagg aggaatcacc agatggaaaa taaaagttat gatatagaag gcatgagttg
     1741 tgcatcgtgc tcccaagccg tggaaaaagc ggcagctaaa gtcgcaggcg taaaggaagc
     1801 caccgtcaat ttggcgaccg agaaattgac ggttactgtc gatgagagcc agttttccga
     1861 aaatcttctg aagaatgcag tcgatgcagc aggttatgtg ttggtggcaa acaagaaaga
     1921 aaagacgttt ctgatcgaag gcatgagttg cgcctcgtgc tcccaaaccg tggaaaaagt
     1981 gacccgggca gtggctggtg tgtcggatgc gtccgtgaac ttggctaccg aaaaaatgtt
     2041 cgtctcttat gatccgacaa tgcttcatcc aggggatatc gaaagagttg tggccgaagc
     2101 gggctataag gcgattgaag agagcagtac tatagaagaa agcgatcaaa atcaagcgaa
     2161 gaaagaacaa cacatcaaaa atatgtggcg gcgcttttgg ctctcggcgg tatttacgat
     2221 tccacttctg tatctcgcga tgggtcacat gttgggattg ccgttgccga tgagcctcca
     2281 tccggatatg catcccgtca cattcgcgct cacacagctc gtgttgacga tcccggtgat
     2341 gctgctcggg acgaaatttt tcacagtcgg cttcaagtcc ttgttcaaag gccacccgaa
     2401 tatggattcg ctcgtggctt tgggaacaag cgcagccttt ctgtatagcc tgtatggcac
     2461 agttgcggca atcggtggga acaacgagct tgtcatgaac ttgtactacg aatcagcagc
     2521 cgtcattttg acgctgatta cgctcggcaa gtacttcgag gccgtttcaa aaggcaagac
     2581 gtcggaggcc atcaaaaaac tgatgggctt ggcgcctaag acagcccgcg tcatccgtga
     2641 cggcaatgag caggaaatta agctggatgc cgttatggtt ggggacattc tggttgtccg
     2701 tcccggcgaa aaaatggctg tcgatgggaa agtcacggaa ggcctgacat ccgtcgatga
     2761 atcgatgctg acaggcgaga gtatgccggt ggagaaaaaa gcgggcgatg ccatcatcgg
     2821 cggcagcatc aataaaaacg gaacgatcca ataccgtgcc gaaaaagtcg ggaaagatac
     2881 ggcgcttgcg cagatcatca agttggtgga agatgcccaa ggttcaaaag caccgatcgc
     2941 aaaactggcc gatacgattt cgggctactt cgttccgatc gtcatcgtgc tcgctgttct
     3001 ggccgggctg gcgtggtact tcatgggaca agaatcctgg atttttgcac tgaccatcac
     3061 gatttccgta ttggtcatcg cttgtccttg cgcgttgggc ttggcgactc cgaccgccat
     3121 catggtgggt accggtaagg gcgccgagaa cggtgtgctg atcaaaagcg ggacggctct
     3181 ggaggtaacg cataaaattc agactatcgt tttcgacaag acggggacga tcacggaagg
     3241 taagccgaaa gtgacggatg ttctggtccg tgaagggctg gatccgaatg aactgttgct
     3301 gttggcagca tcggccgaaa aagggtcaga gcatccgctc ggggaagcca tcgtccgcga
     3361 tgccgaggag aaacagctgt ctttcttgaa accatccgcc ttcaatgcgt tgcctggctt
     3421 cggtatcgct gttacaatcg atgggaaaga gcttctgttg ggaaatgaaa agttgatgct
     3481 gaaccatgca atcggactcg gttcatttgc cgaaacctcc cacacgctgg cggaacaagg
     3541 caaaacaccg atgtacattg caatggatgg ggaattggcc ggcatcatcg ccgttgccga
     3601 tacggtgaaa gcaagcagtg cgaccgcaat caaaaaactc cgtgatatgg gcatcgaaat
     3661 cgcgatgatc accggtgaca ataaacgtac ggcgcaagcc atcgccaacc aggtgggcat
     3721 cgaccgggtc ttgagtgaag tattgcctga agacaaagcg gaagaagtca agaagctgca
     3781 acagggcggc cgcaaagtcg ccatggtcgg agacggtgtc aatgatgcac cggcattggc
     3841 tcaagccgac atcggcatcg ccatcggatc agggactgat gttgccatcg aatcagcaga
     3901 tatcgtactg atgaagagcg acctgatggc ggttgccacg gccgtcgaac tcagcaaagc
     3961 caccatcaaa aacatcaagg agaatctgtt ctgggcattc gcctataatg tcctgggtat
     4021 tcccgtcgcg atgggaatcc tgtatgtctt cggaggacct ttgttgaatc cgatgatcgc
     4081 cggagcagcg atgagtttca gctcggtgtc ggttgtgttg aatgcattac gtctgaaagg
     4141 attcaaacct tccgtctcaa aacggtaaag gatcaataaa tagaagaaaa gaggaaataa
     4201 tgatgaaaaa agaattacta cttgaaggaa tgaaatgcga tggttgcgta gcgacggtca
     4261 aagagaaatt ctctgcgatt gcaggtgtgg aagaggtaat cgttgatctt gaagaaaaaa
     4321 cagcaacggt caaaatgaac caagacattt ccgaaacgac tttcaatgaa gcgttgtccg
     4381 gcacgaagtt taaagtaacc ggcagcaaat aagcaaaaaa taatggcaca ggctccctct
     4441 atgattgctg agggagcttt tttcgcataa atttgtacat tttttctgtt tg
//
LOCUS       DAOMUJ010000101         4447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5510_length_4447_cov_2.846767, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000101 DAOMUJ010000000
VERSION     DAOMUJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_4447_cov_2.846767"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="PLT73_03735"
     CDS             <1..1728
                     /locus_tag="PLT73_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19526.1"
                     /translation="GERQVAEAKQALADLIEPVYYVTDRSGNPGYQEYRDNADRISAI
                     AEIFPVFFFLIAALVSFTTMARMVDEQRQQMGTLKGLGYSDFDIAKKYLIYAAIACIV
                     GTSLGLVAGYNIFPAVIFDAYGSMYSLPSVKITYYLSYALISIAIALLCTIGPAAWAA
                     HASLRENPAMMMRPKAPKNGKRVLLERVTFIWDRLSFNSKITVRNLMRYKARNMMTIL
                     GVAGCTALILTGYGIKNSISGLADTQFNDVMRYNAITAMRPEASAEEIASYDELVAAT
                     PEITDHLKVVQESYKLDKKGVNLQNVTVFAPLESENLPDFVSLRDRITQEPIALTDEG
                     AVISEKLANLADVGPGDSIEIRNDEMQTYQIPIQAVTENYVNHYIYLTPSLYEEIFIQ
                     AAEPTTDLLLFDEPESWERSFGSEVMGEQAVALVTFINSVDRSFAETLGSLDVVTLVL
                     IVSAASLAFVVLYSLTNINVSERIRELSTIKVLGFYDVEVSMYIYRESLVLTLLGILF
                     GFVLGKILSTVVLKMVEIDFMMFPPTIMPISYLYAGLLSLLFSSVVMLIMHRKLKQVD
                     MIEALKSVE"
     gene            complement(1798..2307)
                     /locus_tag="PLT73_03740"
     CDS             complement(1798..2307)
                     /locus_tag="PLT73_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HRM19527.1"
                     /translation="MIHLDKEKFRVGMRTTKSGVAVGFCVLLFKLLDRGSPMLAGLAA
                     VFALREDCSQSLHHGIRRVGGNTVAGVLGVTLIAVKDFLGLDFSVDFFGTIIAIIILI
                     TVCNVLDMSTAIVGATATFLVVFYNIDDAEKITYAIQRVLDTVIGTSIAIGVNYLLPG
                     SPQTPADNC"
     gene            2709..3857
                     /locus_tag="PLT73_03745"
     CDS             2709..3857
                     /locus_tag="PLT73_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19528.1"
                     /translation="MKKKFLMGLASVFVLAACGNGATTEESDTIKLGGNFELSGGASA
                     YGTLMDEGIKMAVAEQNAADGLLGKEIEYVSYDNKSEPSESTSVATRLATEDNVVAIL
                     GPATTGAANAQSPAVTRAKVPAILPAATGDGVTMDGDSVLEYVFRVCFQDSFQGKALA
                     EFAQTDLQATKAVILVDNSTDYAIGLADAFKETFTGDIVAEENFTAGDTDFKAILTNI
                     ANLDYDVIFLPGYYEEGGLIIKQAREMGIVQPILGPDGFANSTLVELAGADNVDNVFY
                     ASHFTPNSEDPKVQDFLAAFEAEYGKPADSFAALAYDAAQLVFDAIANADSTDPQAIT
                     DALAATEDFEGVTGTFSFDENHNPIKSAFVIELQDGVEVGNSIVEPAK"
     gene            4080..>4447
                     /locus_tag="PLT73_03750"
     CDS             4080..>4447
                     /locus_tag="PLT73_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM19529.1"
                     /translation="MENLVQQLINGISLGSIYALIALGYTMVYGVIKLINFAHGDIYM
                     VGAYLGYALIQNVGLGLFPSILISMIFCAILGVVIEKIAYKPLRGATRVATLITAIGV
                     SYLLQNVMIYIMGPEVKAFPT"
BASE COUNT         1231 a          964 c         1100 g         1152 t
ORIGIN      
        1 ggggaacgtc aggtcgcaga agcgaagcaa gccttggccg atctgattga gccggtctac
       61 tacgtgacgg atcggtccgg gaaccctggt taccaggaat accgcgacaa tgcggaccgc
      121 atctcggcca tcgcagagat tttcccggtg tttttcttcc tgattgcggc tttggtcagt
      181 ttcacaacga tggcccggat ggtggatgag cagcgccagc agatggggac gctgaaaggc
      241 ctcggttaca gtgatttcga tatcgccaaa aaatatctga tctatgcagc gatcgcctgc
      301 atcgtaggga cctcactcgg tttggtggcc ggatacaata ttttccctgc ggtcatcttt
      361 gatgcctatg gatccatgta cagtctgcct tccgtaaaga ttacctacta cttgtcctat
      421 gcactgattt ccattgctat cgcgctgctg tgcaccatcg gaccggctgc gtgggcggca
      481 catgcctcgt tgcgggaaaa cccggctatg atgatgcgtc cgaaagcgcc gaagaatggc
      541 aaacgtgtgc ttttggaacg cgtgacgttc atttgggacc gtctcagctt caactcgaag
      601 attacggtac gtaatttgat gcgctacaaa gcgcgcaaca tgatgacgat cttgggcgtt
      661 gccggctgta ccgccttgat attgaccgga tacggtatca aaaattccat ctccggattg
      721 gcggataccc agttcaacga tgtgatgcgc tataacgcaa tcacggcgat gcgtccggaa
      781 gcgagcgcag aagaaatcgc ctcctatgat gaactggtcg ctgcaactcc tgaaataacg
      841 gatcatctca aggtagtgca agaaagctac aaactggata aaaaaggagt aaacctgcag
      901 aacgtcacgg tctttgcgcc gctggaaagc gaaaatctcc cggattttgt cagcttacgc
      961 gatcgtataa cccaagaacc gatcgcgttg acggatgagg gagccgtcat ttcggaaaaa
     1021 ttagcgaacc tggcagatgt cggaccgggc gacagcatcg aaatccgcaa tgatgaaatg
     1081 cagacctatc agattccgat ccaggctgtc accgagaact acgtcaacca ttacatttac
     1141 ttgacgccat ctctttatga agagattttt atccaagctg cggaaccgac aactgatctt
     1201 ttgctgtttg acgaaccgga aagttgggaa cggagcttcg gatctgaagt gatgggagaa
     1261 caggcggtgg cgctggtgac attcattaat tccgtcgatc gcagcttcgc tgaaacctta
     1321 gggagcctgg atgtcgtcac cttggtattg attgtgtcgg cagcctcatt ggctttcgtt
     1381 gtgctgtaca gcctgacgaa catcaatgtt tcggagcgga tcagagaact gtcgacaatt
     1441 aaagttttgg ggttctatga tgtggaagtg agcatgtaca tctacagaga gtcattggtc
     1501 ttaactttgt tgggtatttt gttcggtttt gtcctcggaa aaattttgag tacagtcgtt
     1561 cttaaaatgg tggaaattga cttcatgatg ttcccaccga ccattatgcc gatcagctac
     1621 ctgtatgcag ggttactttc gttgttgttc tcaagtgttg tcatgttgat tatgcaccgc
     1681 aaactgaaac aagtggacat gatcgaagca ctcaagtcag ttgaataaaa gtaagaatag
     1741 aaaaacgcgc aagagccatc aaataggccg cttgcgcgtt tttcttcttt agagacatca
     1801 acaattatcg gctggtgttt gaggtgaacc aggcaatagg tagttgactc cgatggcgat
     1861 ggaagtgccg atgacggtat ccagtaccct ttggatggca taggtgattt tctctgcatc
     1921 gtctatgttg tagaaaacga caagaaaagt cgcggtggct ccgacgatag ctgtggacat
     1981 gtccaggacg ttgcagactg tgatcaggat gatgatggcg atgatcgtac cgaagaaatc
     2041 cacagaaaaa tccagtccga ggaaatcttt gactgcgatc aaggttaccc ccaagacacc
     2101 ggcgacagta ttacctccga cacggcggat gccatgatgc agtgattgtg agcaatcttc
     2161 acgcaaagca aagactgccg ccaagccggc caacataggc gaacctcggt ccaataattt
     2221 gaacagcagc acacaaaagc cgacagcaac gccggatttg gtcgtacgca tgcctacgcg
     2281 gaatttctcc ttatccaaat gaatcatagt aagtacgata cttcctctcg aaatgaaata
     2341 atatctcaat cataagcatt ttttgatttt tatcaaggca ttttcacatt attgtgcaaa
     2401 taatacacct gcatttactt cattgttcag agtagctttt cgaaaaatga gaaaaaaacg
     2461 gctatttttg gataagaaat gtcctttttg gggcaagtca ttttattgga attgttgcaa
     2521 cggcggcttt ttcaggtagg gtgaaaaaag aacattcgct ttttgccaaa aaaggccaaa
     2581 attgggttgc ttaaattgtc agaatattct atactaatga cattaaattt taaggagaag
     2641 gatgagggcc ttttctttga aattgcaacg aattattaat atatagtgag atggggagga
     2701 ttatttagat gaaaaagaaa tttttgatgg gtctagcatc tgtattcgta cttgcagcat
     2761 gcggcaatgg cgcaacgaca gaagagagcg atacaatcaa attgggtggt aacttcgaat
     2821 tatcaggcgg tgcgagtgca tacggcacgc tgatggacga aggaatcaaa atggcggttg
     2881 cagaacagaa cgctgcagat ggcttattgg gtaaagagat tgagtacgta tcttacgata
     2941 ataaatctga gccttcagaa tccacatcgg ttgcgactcg tttagctacc gaagataatg
     3001 ttgtcgcaat cttaggacct gctacaacgg gtgcagcgaa tgcacaatct ccagcagtca
     3061 caagagcaaa agtgccagca atccttccgg ctgctacagg cgatggcgta acgatggatg
     3121 gcgattccgt tctggaatac gtattccgcg tatgtttcca ggattctttc caaggtaaag
     3181 ctttggcaga attcgctcaa acagacctgc aagccacaaa agctgtcatc ttagtggata
     3241 attcgactga ctacgcgatc ggcttggctg atgcgttcaa agaaacattc actggagaca
     3301 tcgtagcgga agaaaacttc acagccggcg atacagactt caaagcaata ctgacgaata
     3361 tcgcaaatct ggactatgat gtcatcttcc tgcctggata ctatgaagag ggcggattga
     3421 tcatcaaaca agcccgcgaa atgggtattg tgcaaccgat cctaggaccg gatggcttcg
     3481 ctaactcaac cttggttgaa ttggcaggtg cagacaatgt ggacaatgta ttctacgcga
     3541 gccactttac tccgaacagt gaagatccta aagtacaaga cttcttggct gcttttgaag
     3601 cagagtacgg caagcctgca gattcattcg cagctttggc ttacgatgct gcgcaattgg
     3661 tattcgatgc aattgcgaat gctgacagca cagatcctca agcaatcaca gatgctttag
     3721 cggcaactga agatttcgaa ggcgtaacgg gtaccttctc atttgatgag aaccacaacc
     3781 ccatcaaatc cgctttcgta atcgaattgc aagatggtgt agaagtcggc aattctattg
     3841 tggaaccagc taaataaaaa cactggtgcg cgagattgtg gcattgaaac ctgaatagac
     3901 cgtttgattc caaagtaaca caatgaggtg ctggcagaat ttgcttagca cctttcttta
     3961 tgttttacct catcgtaaag taatccctca tcgtgcgtga ataatagtaa actatatccg
     4021 aacaatgcag tataataaat aaaagacttc tgtccagcag aagaaaagag aggtgtaaaa
     4081 tggaaaattt agttcaacag ctgataaacg gtatatcgtt gggaagtatc tacgccctga
     4141 ttgcgctagg gtacaccatg gtatacggtg tcatcaaatt gattaacttt gcccacgggg
     4201 acatttacat ggtgggcgcg taccttggat atgctttgat ccaaaacgtc ggcctcggcc
     4261 ttttcccatc cattctgatt tcaatgattt tttgtgccat tttaggggtg gtcatcgaga
     4321 agatcgccta caaaccattg cggggcgcga ccagggtagc gacactgatt acagccatcg
     4381 gggtttctta tttattgcaa aatgtcatga tctatataat ggggcctgaa gtgaaggcct
     4441 tcccgac
//
LOCUS       DAOMUJ010000102         4438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5521_length_4438_cov_1.861967, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000102 DAOMUJ010000000
VERSION     DAOMUJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5521_length_4438_cov_1.861967"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>486)
                     /locus_tag="PLT73_03755"
     CDS             complement(<1..>486)
                     /locus_tag="PLT73_03755"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM19530.1"
                     /translation="DYQPDYISIRGKGDLIKELKKYGHKAEKIYLASDPDREGEAISW
                     HLAHLLGLDPADNIRVVYNEVTKDAVKEALKNPRPINMDLVSAQQARRILDRLVGYSI
                     SPILWKKVKKGLSAGRVQSVALKLIINREEEIAGFEPVESWTIDGTFLKDKHVFQAAF
                     YG"
     assembly_gap    487..496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<497..635)
                     /locus_tag="PLT73_03760"
     CDS             complement(<497..635)
                     /locus_tag="PLT73_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM19531.1"
                     /translation="MAYKYLVIVESPTKAKTIEKYLGRNYKVVASKGHLRDLPKSRMA
                     VD"
     gene            complement(751..1659)
                     /gene="dprA"
                     /locus_tag="PLT73_03765"
     CDS             complement(751..1659)
                     /gene="dprA"
                     /locus_tag="PLT73_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRM19532.1"
                     /translation="MELNHEDLTVRDKLIYHAMTGVCTPHFMKHLYLSWMRDPDYSIE
                     ENIKGYVPMANESKQQKLKSAIQTAYSRMSYEELLELYRSNDIQILTILDNGYPEGWF
                     NSYLPPIVVFCAGNIGLLEQPCLSVVGSRSPSAYGKAVLNEMIPPLVVEGITMVSGLA
                     KGIDKMVHAYAIENGGATIGVIGTGLDVTYPRENASLQRKMMAEQLVISEYPLGSKPE
                     RYHFPMRNRLIANLSSATLVIEATEKSGSLITANLALQENRDVFAVPGNITSHLSVGT
                     NQLIKAGAACVLNATDVLEEMRTQWN"
     gene            complement(1755..2514)
                     /locus_tag="PLT73_03770"
                     /pseudo
     CDS             complement(1755..2514)
                     /locus_tag="PLT73_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315203.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            complement(2511..3389)
                     /gene="ylqF"
                     /locus_tag="PLT73_03775"
     CDS             complement(2511..3389)
                     /gene="ylqF"
                     /locus_tag="PLT73_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRM19533.1"
                     /translation="MLIQWFPGHMAKAKREALEKLKMVDLVIELLDARLPESSRNPLI
                     DDIIGNKPRLIVLNKADLADKTATKEWVEYFKQDNKAQRAVSISTLNNKDVENIKREL
                     KAMMAHKTQAMIAKGVKPRAIRLMILGIPNVGKSTLINKLMQKNRAQTGNRPGVTKGQ
                     QWLKIGGDFELLDTPGILWPKFEDQLIGQKLALTGAIKDTVFHKDDVALFALRHFMQY
                     YPGRLQAFYKVDEKEFEGAVPDLLMELTTKLRFGEDYSRASERIIFDIRGGKLGPYTL
                     DQVPVKLAEAGEDLAE"
     gene            complement(3476..4138)
                     /gene="lepB"
                     /locus_tag="PLT73_03780"
     CDS             complement(3476..4138)
                     /gene="lepB"
                     /locus_tag="PLT73_03780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM19534.1"
                     /translation="MSFGNKNHDYQDSGFVSRNERFGVEQKKDIQPEKRGGFFREALS
                     LLLSVAIAFVLFVVIRTYLFYPFQVVGDSMVPTLETGDRLILNKLAEVDRFDIVVFPA
                     PDGTDEEYVKRVIGLPGDEITYFQDELYINGEKVTEHYLEALKEGSNQTLTGDFTLFS
                     LTGEMAVPEDMYFVLGDNRVVSKDSRVFGFIPAAQIEGTADLRLWPLNKIGIIADNGA
                     EE"
     gene            complement(4172..>4438)
                     /gene="lepB"
                     /locus_tag="PLT73_03785"
     CDS             complement(4172..>4438)
                     /gene="lepB"
                     /locus_tag="PLT73_03785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM19535.1"
                     /translation="QLYLNGEVIEEPFLGTEQQQGMDVFTSDFDLFSLTGVEIVPEDS
                     YFVLGDNRVRSRDSRIFGFVPQADIAGKAAMVYFPFEDFGFIEN"
BASE COUNT         1105 a         1138 c          913 g         1272 t
ORIGIN      
        1 cccgtaaaat gccgcctgaa aaacatgttt gtccttcagg aaagtgccgt ctattgtcca
       61 agactccaca ggctcgaaac cggcaatctc ttcctcacgg ttgatgatca gtttcagcgc
      121 aacggactga acccgtccgg cactcaaacc ttttttgact ttcttccata gtatgggtga
      181 aatggagtac cctaccagac gatccaagat tcttcgggcc tgctgggctg agacaagatc
      241 catattgatc ggtcttgggt ttttaagtgc ttctttaact gcatctttgg ttacttcatt
      301 ataaacgaca cggatattgt ctgctgggtc caagcccaac aaatgtgcaa gatgccatga
      361 gatggcctcc ccttctctat ccgggtcgga agccaagtaa attttttctg ccttgtgtcc
      421 atattttttc aattctttta taaggtcgcc tttaccacgg atggaaatat agtccggttg
      481 gtagtcnnnn nnnnnntatc aaccgccatt cgactctttg gtaaatcgcg taagtggcct
      541 ttgctcgcta ccactttata gtttctgcca agatactttt ctatcgtttt ggctttagta
      601 ggtgattcca cgatgactaa atatttataa gccaaagaaa cgactcctct caacgaaata
      661 ttttgtatgt atatttctgt catttaaatc gtttcccatc ttatgccata aactaggtgt
      721 ttgtcaaatg gaagttattt cgaagatgct tcagttccac tgcgttctca tttcctccag
      781 cacgtcagtc gcattcaaaa cgcaagcagc accggccttt atcaattgat tggtaccgac
      841 gctgaggtgg ctggtaatat ttcctgggac cgcaaaaaca tctcgatttt cctgcaaggc
      901 tagattcgca gttataaggc ttccactctt ttctgtcgcc tcgatcacca gtgttgctga
      961 tgagagattg gcgatcaagc gatttcgcat cggaaaatga tagcgttccg gctttgagcc
     1021 caaaggatac tcactgatga caagctgttc cgccatcatt ttccgctgaa gggaagcatt
     1081 ttctcttgga taggtgacat ccaaccctgt tccgatgacc ccgatcgttg ctccaccgtt
     1141 ttcgattgca taagcatgaa ccatcttatc gattcccttg gcgagcccgc tcaccatcgt
     1201 gatgccctcc acaaccaacg gcggtatcat ttcattcagg actgcctttc cataggcgga
     1261 cggactgcgg ctgcctacga cacttaaaca cggctgctcc aataaaccga tattacccgc
     1321 gcagaacacc acaattggag gcagatagct attaaaccag ccctccggat aaccattatc
     1381 cagaatggtc aaaatctgaa tatcattgct ccggtaaagt tccagtaatt cctcataaga
     1441 catcctgctg taggctgttt gtatggctga ctttagtttc tgctgttttg attcattggc
     1501 catcggaaca taccctttga tgttctcttc tattgaataa tccggatccc tcatccaaga
     1561 caaatagaga tgcttcatga aatgtggcgt gcacacgcca gtcattgcat gataaatcaa
     1621 cttatcgcgg accgtcagat cctcgtggtt caattccata aaaaaactcc tttgcgtaca
     1681 atctgataag ataatacgca aggagttgca ttaagcttta ttcaattgtt tgcatttgga
     1741 gtacagaatg tttactaatc tctcagcatg cttttgatag gatcaaacgt cttccggtgg
     1801 ataggggtaa taccatgcga ttccaaaccg gataaatgca gttgggtgcc atagccggcg
     1861 ttcttctcaa aaccatagta aggatattga atcgcatatt cggacatcag gcgatcacgg
     1921 tatactttcg cgacaatgct cgccgcagcg atggagaccg atttggcatc acctttaatg
     1981 atcgattgct gctcgatcgg caaaggaagc ttcatggcat cgatcagcag gctgtccggt
     2041 tcttgtttca gattggcgat cgcctgcatc atcgccagtt tcgtggcttc ataaatattc
     2101 acggcatcaa tcgtttccgg ggaaatcaat ccgatcccga catcggcaat ggccatgatc
     2161 tgatcgtata gttgctccct tttttttgca gagagttgtt tcgaatcgtt gaccggaagg
     2221 aaagggagct ctgccggcag aatgacggcg gcagcgacca ccgggccggc taacgggcct
     2281 ctgccgactt catcgatacc tgcgatggca agccggccat ttgccttcag ttcctcttcg
     2341 aaggccatca tctggatttg ccgttctttc aaggcgaggt cgagttgata attccgctcc
     2401 cacgatttga tcgcggcttg gacgcctttg cgggagtcgg ctcggatcgt tgtccaacga
     2461 tcggatttcc tgcaactgct ccttgatggc agcgatgctc aatggtttgt tcattctgct
     2521 aagtcctctc cagcctccgc cagtttaacg ggaacctgat ccaaagtata aggcccaagt
     2581 ttgccgccgc ggatatcaaa aatgattctc tcgctggcac ggctgtaatc ctcgccaaag
     2641 cgcagtttcg tggtcaattc catcaacagg tccggaaccg ctccttcgaa ttctttttcg
     2701 tccaccttat agaatgcctg caatctgcct ggataatact gcataaaatg acgcaacgcg
     2761 aataaagcga catcatcctt gtggaaaacg gtatccttga ttgctccggt tagggcaagt
     2821 ttttgaccga tcagctgatc ctcgaattta ggccacagta tccccggagt gtccaacaac
     2881 tcgaaatcac cgccgatttt cagccattgt tgcccttttg tcacaccagg tcggtttccg
     2941 gtctgggctc gattcttctg catcaattta ttgatcaaag ttgacttccc gacgttaggg
     3001 atgccaagga tcatcaaacg gatggcgcga ggtttgacgc ctttagcgat catcgcctgc
     3061 gttttatgcg ccatcatcgc tttcagttct cttttgatgt tctctacatc tttattgtta
     3121 agcgtcgaaa tcgaaacggc tcgctgcgct ttgttatcct gtttgaagta ttccacccat
     3181 tcttttgtgg ctgtcttatc cgcaaggtca gccttattga gcacaatcaa tcttggtttg
     3241 tttccgatga tatcgtcgat caatgggttc cgactcgatt ccggaaggcg cgcatccagc
     3301 aactcaatca ccaaatcgac catttttaat ttttctaacg cttcacgttt cgcttttgcc
     3361 atgtggcctg gaaaccattg tattaacata ttgacacttc cttttctaag taaatccttc
     3421 gcttccattt caagacaaaa cacaagtgca aatacacttg tgtgcggttg tctctttatt
     3481 cttctgcccc attatcagca atgatcccta ttttgtttaa aggccacagt cttaaatcag
     3541 ctgtcccttc gatttgcgct gcggggataa atccgaagac acgactatct ttcgaaacaa
     3601 cgcggttgtc cccaagaaca aaatacatgt cttcagggac tgccatttcc cccgtcaggc
     3661 taaagagggt gaaatcaccg gtcaatgtct gatttgatcc ttcttttaaa gcctccagat
     3721 aatgttccgt tactttttcg ccgttgatat acagttcatc ctggaaataa gtgatttcgt
     3781 caccaggaag cccgatgacg cgtttcacat attcttcatc cgtcccgtca ggagcaggaa
     3841 acacgacgat atcaaaacga tcaacttcgg ccaatttgtt caggatcagc cgatctcccg
     3901 tctccaaagt gggtaccatc gaatcaccga caacctggaa cggataaaac agataagtcc
     3961 ggatgacaac gaacagtaca aaggcaatcg ccaccgaaag aagcaggctc agcgcttccc
     4021 gaaaaaagcc acctcgtttt tccggttgaa tatctttctt ttgttcgact ccgaatcttt
     4081 cgttccttga aacaaaacca gagtcttgat aatcatgatt tttatttcca aatgacatcg
     4141 aatcaccctt tcttataaac ccagtcggtt cttaattttc aataaagcca aaatcctcaa
     4201 acggaaaata caccattgca gcttttccgg ctatatcagc ctgtggcaca aaaccgaata
     4261 tccggctgtc ccggctacgc actcgattgt ccccaagcac aaaataacta tcctccggga
     4321 ctatctcaac tcctgtcaag gaaaacaaat caaaatcaga agtgaaaaca tccattccct
     4381 gttgctgctc cgtcccaaga aaaggctctt cgatgacctc tccattcaga tacagctg
//
LOCUS       DAOMUJ010000103         4425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5547_length_4425_cov_2.723341, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000103 DAOMUJ010000000
VERSION     DAOMUJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_4425_cov_2.723341"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..623)
                     /gene="msrA"
                     /locus_tag="PLT73_03790"
     CDS             complement(75..623)
                     /gene="msrA"
                     /locus_tag="PLT73_03790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRM19536.1"
                     /translation="MSKEIATLAGGCFWCMLEPFDERPGIESVVSGYTGGFKENPTYE
                     EVKAGGTGHTEAIQITFDNSVISYGELLDIYWQQTDPTDAMGQFMDRGSSYRPEIFYH
                     SETQKEIAERSKEELKDKLAIAATIVTQISPASIFYPAEEYHQDFYKKEGNHERMIPL
                     EEDRQNRLNEVWGEADKHEKNI"
     gene            872..1738
                     /locus_tag="PLT73_03795"
     CDS             872..1738
                     /locus_tag="PLT73_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM19537.1"
                     /translation="MQKTKNQTQIYAFVTVLLWASAFVFTKIALTYYTAEAIGVLRYV
                     IASLLFIVIGVFQKIGLPEKKDIPKFLLAGALGFSLYMITFNQASKSLTAATGSVVIA
                     SAPILTALFAWMIFKERIRSLGWVAIIIEFIGILILTLWNGILSVNAGIFWMFGAAVC
                     ISGYNLLQRSLLKKYSALQSTAYSVLAGTLFLLWYLPDAAVQFGNAPLQQLLVMIFLG
                     VFPSALAYMLWAKALMLAQKTSEVTNYMFVTPLLAALMGFLIMGEIPALSTWLGGLMI
                     FAGLLLFKKANT"
     gene            complement(1964..3352)
                     /locus_tag="PLT73_03800"
     CDS             complement(1964..3352)
                     /locus_tag="PLT73_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRM19538.1"
                     /translation="MLNKKIILESIPEPQVFEHVAPTFLNAETIAERKQKLLQRMSEE
                     NFEALIIYADKEHASNFEYLTGFIPRFEEGLLVVENPEKFTLILGNENLKLCAHARVP
                     ADLIHYPQFSLPNQPMENEKTLESIFLDLGIAKKKRVGVIGWKMFTTKQSDPSTLFDV
                     PYFIVDALKNTIPTECELVNGAYVMIGDNGVRTTNNANEIAHYEYGANLSSRCMLRAM
                     NAIEPGCKETEIGNLLNADGQYNSVVTIAAAGQRFELANIYPTHKEIQLGEPMSLTTG
                     FKGGLSSRTGFVVEQAEQLPDTQRDYLEAVAKPYFAAVVSWLENIKVGMAGNELYQLI
                     EDVFPKSKYHWHLNPGHLTADEEWMSSPIYADSSEKLKSGMILQIDIIPSVPGYTGVS
                     AEECVAIADENLQHEIQKSYPELWERISVRRDYLTNVLNINLSADVLPLSNTVAYLRP
                     FYLAKEKAFKIE"
     gene            complement(3433..4176)
                     /gene="map"
                     /locus_tag="PLT73_03805"
     CDS             complement(3433..4176)
                     /gene="map"
                     /locus_tag="PLT73_03805"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRM19539.1"
                     /translation="MIAQTEEDFNGLKEIGGICGAIRDEMVKATKPGITTKELDDIAK
                     ELFEKAGAQSAPKGEYDFPGYTCISVNEEVAHGIPSDRIIEEGDLVNIDVSGSKNGYF
                     ADTGISFVVGEGHVMLQKICEVAKEAFDAGLAKVKPGAKTSNLGKAVHNVAKRHGLTV
                     IKNLTGHGVGRAIHEAPDHIFNYYSRWDDEILKEGMVIAFEPFISTFEEEVFQVEDDD
                     WTFFTEESMVAQYEHTIIVTKEGPIVITL"
BASE COUNT         1109 a         1056 c          900 g         1360 t
ORIGIN      
        1 ggatatcttc ttgagccgtt tcctggccgc atgcagcgag aaacaatgcc gctgccccaa
       61 ccgttactaa acttttaaat atttttttca tgtttgtccg cctctcccca tacttcgttt
      121 aagcgatttt gcctgtcttc ttccaaaggg atcattcgct catgatttcc ctctttttta
      181 taaaaatcct gatggtactc ttccgcagga taaaagatgc ttgcaggtga aatttgcgtg
      241 acgatcgttg cagcgattgc cagcttatct ttcagctcct cttttgagcg ttccgcaatt
      301 tctttctgtg tttccgagtg ataaaagatt tccggacggt acgacgagcc gcggtccatg
      361 aactgtccca tggcatctgt cggatccgtt tgttgccaat aaatatccag caactcccca
      421 taggaaatca cactattatc aaaagtgatc tggatcgcct ctgtgtgccc ggttcctcct
      481 gccttcactt cctcatacgt cggattttct ttgaaaccgc cagtatagcc ggaaacaacg
      541 gactcgattc caggtctttc atcaaacggt tcaagcatgc accagaaaca tcctccggca
      601 agcgttgcaa tttctttgga catagtctta ttctccccca aaaatggcgt tccgccaatt
      661 cataacattt tttcattata tagcaggaag ttacttttag atagtgaaac aaaatcgatc
      721 gttttttccg aacaccacag aatgtccctt ctgaaaatca ttgacttttc ctgtaattaa
      781 attatcctat aaacatacca gaaatgagtg ttatgaaaat cgatgcgttt tcatcggaag
      841 cgtattgatg attcaggata gcgagggaaa catgcaaaag acaaaaaatc agacacaaat
      901 ctatgccttt gttacagtgc tgctgtgggc atcggcattt gttttcacaa aaatcgcgct
      961 gacttactac acggcagaag cgataggcgt tctccgttat gtgatcgctt cgctattatt
     1021 cattgtaatc ggggttttcc aaaaaattgg attgccggag aaaaaagata tccctaaatt
     1081 cctgctggcc ggtgctttag ggttttcgct ctatatgatc accttcaacc aggcttcaaa
     1141 atcgctgacg gccgcaacag gcagtgttgt catcgcctcc gcacctatct tgacggccct
     1201 gtttgcttgg atgatattca aggaacgaat ccgctcgctg ggatgggtcg caatcatcat
     1261 tgaattcatc ggaatactga ttttgacatt atggaatggt atcctttcgg tgaacgcagg
     1321 tattttctgg atgtttggcg cagccgtctg catcagtggc tataatctgc tgcaacgttc
     1381 cttgttgaaa aaatactctg ccttacaatc aacagcttac agcgttttgg ctggaaccct
     1441 gtttctgctg tggtatttac cggatgcggc ggtccaattc ggcaatgccc cacttcagca
     1501 gttgctggtg atgatttttc tgggcgtatt ccccagtgca cttgcgtata tgttgtgggc
     1561 aaaagcgctg atgctcgcac aaaaaacaag cgaggtgacg aattacatgt ttgttacgcc
     1621 attgctggcc gctttgatgg gcttcctcat aatgggagag atccctgcat tgtcgacatg
     1681 gttgggcggc ctgatgatct ttgccggctt gttgctgttc aaaaaagcca atacgtgata
     1741 ggcacatcgt caacaacaac gttatcttag aggatatgtt cgcaaagatt cgctatcatt
     1801 tcggcgactg attcgttgaa tggatttttg ttaaagcata tgaaaagcgc cttccgatac
     1861 ggactttcgt atcgagaggc gcttttgcta tcttcacttt tgaatccaaa cttagcttct
     1921 gctgacatcc cttggaaaaa agaatgctag tgcttgaagt tagttattct atcttaaaag
     1981 ctttttcctt cgctaaatag aatgggcgta aatacgcgac tgtgttggat aatggcaaca
     2041 catccgcact tagattgata ttcaatacat ttgttaaata gtcccttctg acagaaatcc
     2101 gttcccataa ttccggatag gatttttgta tttcatgctg gaggttttca tcagctatag
     2161 caacgcactc ttcggcactc actccggtat acccaggtac cgatgggatg atatcgatct
     2221 gcagaatcat gccgcttttc agtttctcag aagaatctgc atagattggg gaggacatcc
     2281 attcttcatc cgctgtcaga tgccctggat tcaaatgcca atgatatttg cttttaggga
     2341 atacatcctc aataagctgg tacaattcat ttccggccat tccaactttt atgttttcca
     2401 gccaactgac tacagctgca aaatacggtt ttgctacggc ttccaaataa tctctttgcg
     2461 tatccggaag ttgctctgct tgctcaacca caaacccggt tcgactggat aaaccacctt
     2521 tgaatccggt ggtcagggac ataggctcgc ccaactgaat ttctttatga gtgggatata
     2581 tattggcaag ttcaaagcgc tgccctgctg ccgcaatcgt gacgactgaa ttgtactgcc
     2641 catcggcgtt caacaaattt ccgatttctg tttctttgca gcccggttca atagcattca
     2701 tggcacgtaa catacagcgc gaagacaggt tagcgccata ttcgtaatgc gcgatttcgt
     2761 tggcgttatt cgtggtccgg actccgttat ccccaatcat cacatacgca ccattaacta
     2821 actcacactc tgttgggatg gtatttttga gggcatctac aataaaatac ggtacatcaa
     2881 acagtgtgga cggatcgctt tgcttcgtcg taaacatttt ccagccgatc acaccgaccc
     2941 gtttcttttt ggcgataccc aaatcaagga atatggattc aagtgttttt tcattctcca
     3001 tcggctggtt aggcaatgag aactgtgggt aatggatcaa atcggccgga acacgtgcat
     3061 gcgcacatag ttttaagttc tcattgccaa gaatcaaagt aaatttctct ggattttcga
     3121 caactagcag accttcttca aaacggggaa taaaaccggt taaatattca aaattgcttg
     3181 catgctcttt atccgcataa ataatcaaag cttcaaagtt ttcttcactc attctctgca
     3241 acagtttttg tttccgttca gcaatcgttt cagcatttaa aaaagtaggg gcaacatgct
     3301 cgaacacttg cggctcgggt atactctcta atataatttt tttgttcagc atactattct
     3361 cctctttcgt ctttgataat agcttttacg tcactttaaa atcaacttcc gcactcttat
     3421 gttttgattg ccttacaacg tgataacgat cggaccttcc ttcgtcacaa taatcgtatg
     3481 ttcgtattgg gcgaccatgc tttcttctgt aaagaaggtc cagtcgtcat cttcaacttg
     3541 gaaaacttcc tcttcgaatg tggaaatgaa aggctcgaag gcgatgacca tgccctcttt
     3601 cagaatttcg tcatcccagc gcgagtagta attgaagata tgatccggtg cttcgtggat
     3661 tgcgcgtccg acaccgtgtc cggtcagatt tttgatgacc gtcaagccat ggcgtttcgc
     3721 cacgttatgc accgcttttc ctaagttgct tgtttttgcg cccggtttta ccttcgcaag
     3781 tcccgcgtcg aatgcttcct tggcgacttc gcagattttc tgcagcatga catgcccctc
     3841 accgactaca aaggaaatgc cggtatccgc aaaatagccg tttttggaac ctgaaacatc
     3901 gatattcaca aggtcccctt cttcaatgat ccgatcgctc ggaatcccgt gcgccacttc
     3961 ttcattcacg ctgatgcacg tatatccagg gaaatcgtat tccccttttg gtgccgattg
     4021 cgctcctgct ttttcaaaaa gctccttcgc gatatcatca agctccttag tggtgatgcc
     4081 gggttttgtg gctttcacca tttcatcccg gatcgctccg caaatgccgc caatttcttt
     4141 taatccgtta aaatcctctt ccgtctgtgc aatcattttg tatcctcact tcttaaagca
     4201 tttttgatta tctttccagt tagattctat cacaaacgga agaaatattt cttcagcgat
     4261 accaaataaa gctatctctt tttggatggc aaaaattttt taacaatcca ttttgcgcag
     4321 aaaaatccta tcgccaacga gccgagtaat agcagcagcg aagggagtaa cttgttgagt
     4381 ccctcgccat agaagtaatc gtgaaaaatc tgcatgcctg cgaat
//
LOCUS       DAOMUJ010000104         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5558_length_4419_cov_2.023602, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000104 DAOMUJ010000000
VERSION     DAOMUJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_4419_cov_2.023602"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLT73_03810"
     CDS             complement(<1..336)
                     /locus_tag="PLT73_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRM19540.1"
                     /translation="MLVMAGMIGAGKSTYTEMISRRLGTEAFFESVDYNPILDKFYDN
                     PQKWAFSLQIYFLNTRFRSIKAALTDDNNVLDRSIYEDALFTRVNHLQGNISQEEMDI
                     YNDLLANMME"
     gene            575..1546
                     /locus_tag="PLT73_03815"
     CDS             575..1546
                     /locus_tag="PLT73_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HRM19541.1"
                     /translation="MTLSHRTIKIAFATVIAILIAQFLNLNYSVSAGIIAILSVLDTK
                     KSSVLTALQRIASTVLALTIATVLFRIFGFHIIVFGVYLLFYVPLAYRFTLQSGIAPC
                     SVLVTHLLLEKQTSLPWILNELALMMIGAGVAILFNLYMPSKASQIILLREQVETQMK
                     EVLHSFSVTLREGDDRNQKRLLNELDDQLEKYRQVVYAEFDNQLLDQSTYNIRYFDMR
                     SEQVTILKYMATNLGLCALPTSENKILAGLFFLTAAQLHEQNTGIYLMEDIDSLLQAF
                     RESELPATRAEFENRAILFQLLNDFRRFIQTKKIFYEEYAAEIKTSK"
     gene            1958..3058
                     /locus_tag="PLT73_03820"
     CDS             1958..3058
                     /locus_tag="PLT73_03820"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020282014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM19542.1"
                     /translation="MTTAQKTTTLPFRADHVGSFLRTEPLKDARLKFAGGEIDAAALD
                     QIETEEITKLVKDQKENGLKGFTDGEFRRSWWHIDFIENLNGFEGYVPEHGYDFGDVE
                     VRKYAFRNVGKISFNPNHPFLAAFKSLNEIVGDQGVAKFTIPSPNEVFYPGYRNEEIY
                     PSVEAYQADVQQAYIDTVQALYNLGCRYLQLDDVHWGFLCNFADDSEEYKASKRVAAE
                     NVQAIIEAKPADLVLTTHVCRGNYKSHHSLEGAYDPIAEELFGQTSFDGYFLEYDTDR
                     SGGFEPLAYFKGKGRIVLGLVTSKTAELEDKEEIKARIKEASKYVPLDQLALSTQCGF
                     ASTEEGNLLTEEEQWAKVRHVVEISKEVWPEN"
     gene            complement(3291..>3492)
                     /locus_tag="PLT73_03825"
     CDS             complement(3291..>3492)
                     /locus_tag="PLT73_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19543.1"
                     /translation="IQSLDILIRQMDRQFTKQADIQVNFPIEPQLVFKVGAGQDVIAI
                     ANEMLRNNPIDYSLLENHRNTD"
     assembly_gap    3493..3502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3503..4209)
                     /locus_tag="PLT73_03830"
     CDS             complement(<3503..4209)
                     /locus_tag="PLT73_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM19544.1"
                     /translation="MESPEKSEKGKGLFGISLAGGGLKSFAQLAVLHDLEKNHVKIDA
                     AAGTSMGAVIAALVACGLTATEIEKTLLDAEREFADRGVFSRPAYLLLQHVREHNNGF
                     VELQNLQQMACQLFDKIGCRMLSDCRLPIAITTVDIHTSELIVFTNEPDFFTSPTGDW
                     LIYPHDIELGIAVAASCAFPMIFSTATLDGRQLVDGGVMMNLPIPLFDRSRFSVLMSV
                     SMIYDAVDRQLGSPFNVA"
BASE COUNT         1236 a         1076 c          984 g         1113 t
ORIGIN      
        1 ttccatcata ttggccaaca ggtcgttgta gatatccatc tcttcttggg agatgttgcc
       61 ttgcaaatga ttgaccctcg tgaacagcgc gtcttcatat atggaacggt ccaagacatt
      121 attgtcatca gtcaaagcag ctttgatgct gcggaaccgc gtattcaaga aatatatttg
      181 aaggctgaat gcccattttt gcggattatc ataaaatttg tccaagatcg ggttgtaatc
      241 gacgctttcg aaaaaggcct ccgtacccaa tcttcttgaa atcatttcag tataagtcga
      301 cttcccagca cctatcattc ctgccattac cagcacgctc aatcatatcc cttcctttgg
      361 tgttatctga ttatttccgt aatttaatgt aatttccgat ccgctggttc gcggaacaac
      421 atctagtatc ataccatgcc agcaaaacga gatatagtat ttttttgatt agaaaatcca
      481 cttccagacc gaaaaaggcg cactcttact gtatccgaaa acactggtca atcgaactga
      541 aatgcactat gctagaaata taggagggaa gcccatgaca ttgagccacc gcaccatcaa
      601 aatcgcattt gcgactgtca ttgcgattct gattgcgcaa tttctaaatt tgaattattc
      661 cgtttcagct gggatcatcg cgatccttag tgtgctggac accaaaaaat catccgttct
      721 gacagccttg cagcggattg cttccacagt gctggcgttg acgatcgcaa cggtcctgtt
      781 ccggattttt ggattccaca tcatcgtgtt cggagtttat ctgcttttct atgtaccatt
      841 ggcctaccgc ttcactcttc aatccgggat cgcgccttgt tcggtgcttg tgacgcatct
      901 attactggaa aagcaaacaa gccttccttg gattctgaac gaattagcct tgatgatgat
      961 cggagccggc gtggcgattc tgttcaatct ctatatgcca tccaaagcga gccagatcat
     1021 cttgcttcgc gaacaagtcg agacgcagat gaaggaagtg ctgcacagtt tcagcgtcac
     1081 ccttcgtgag ggagatgacc gcaatcaaaa gcggttgttg aatgagttgg atgatcaatt
     1141 ggaaaaatac aggcaagttg tctatgccga attcgataat cagttgctgg accaatcgac
     1201 ctataacatc cgctacttcg atatgcgcag cgagcaggtc accatcctga aatatatggc
     1261 caccaatctc ggcttatgcg cgctgccgac aagcgaaaac aagattttgg ccgggctgtt
     1321 cttcctgact gccgctcagc tgcacgaaca gaacaccgga atctatctga tggaggacat
     1381 cgattcattg ctgcaagcat tccgtgagag cgagttgccg gccacccggg cggagttcga
     1441 aaatcgcgcc attttgtttc agttgctgaa cgacttcaga cggttcatcc agactaaaaa
     1501 aatattctac gaggaatacg cagcggaaat caaaacaagt aaatgaattt atcgtgagaa
     1561 aatatttgac acttactaat catattgtta ttatatatat aaattcaaac aacctcacga
     1621 tgaaaagaag agtatggata acggaaatgc acagagaacc tcggcagctg agaagaggac
     1681 aggaaagtca tccagaaaat ggtcttagag tgattggttt gtgaacgcca tccggcctca
     1741 gcgagccacg tccgcatacg ttacaatgcc gcagtataac aagactatcg atcgtcttgc
     1801 gtactgactg agggggcatg cgtgagcgta ccccgaacca aggtggtatc acgatgagca
     1861 atcatacgtc cttgcacagc aatgtgcagg gacgtatttt tttttactgc attatcttgt
     1921 ttgataaaaa aataataacg gaaagaagga aaataatatg acaacagcac aaaaaacaac
     1981 aactttacca ttcagagccg atcacgtagg gagcttttta cgcacggaac cattgaagga
     2041 cgcgcgcttg aaatttgcag gcggcgaaat cgacgctgct gctttggacc aaatcgaaac
     2101 ggaagaaatc acgaaactgg tcaaagatca aaaggaaaac ggattgaagg gattcacgga
     2161 tggcgaattc cgtcgctcat ggtggcatat cgatttcatc gaaaacctga acggatttga
     2221 aggctatgtg ccggaacacg gttatgattt cggggatgtg gaagtccgca agtacgcttt
     2281 ccgcaacgtc ggcaaaatct ccttcaatcc taaccaccct ttcctggctg ctttcaaatc
     2341 attaaatgaa atcgtcggag accaaggcgt agcgaagttc acaatcccta gcccgaacga
     2401 agttttctac ccaggctatc gcaacgaaga aatctaccct tcggttgaag cgtaccaagc
     2461 ggacgtgcaa caagcctaca tcgacactgt ccaagcttta tacaacttgg gctgtcgcta
     2521 tctgcaattg gacgatgtac actggggctt cctttgcaac ttcgcggatg attcggaaga
     2581 atacaaagct tccaaacgcg ttgcagctga gaacgttcaa gccatcatcg aagcaaaacc
     2641 tgctgatctt gtcttgacga cacatgtctg tcgcggcaac tataaatctc accactcatt
     2701 ggaaggcgct tatgatccga tcgcagaaga gcttttcgga caaacaagct tcgacggtta
     2761 tttcctggag tatgataccg atcgttccgg cggtttcgaa ccacttgcct acttcaaagg
     2821 caaaggccgt atcgtattgg gcctggttac ttcgaaaacg gcggagctgg aagacaaaga
     2881 agaaatcaaa gcccgcataa aagaagccag taaatatgtt cctttggatc aattggcgtt
     2941 gagcacgcaa tgcggattcg cctccacaga agaaggtaac ttgctgacgg aagaggaaca
     3001 atgggcgaaa gtacgccacg ttgtagaaat ctccaaagaa gtttggccgg aaaactaagc
     3061 attatatgca aaaccgcaca atcagctgat gattgtgcgg ttttttttat tgtagcgtca
     3121 gctgcaaggc tgacctccgg tgagacgctg ttcgccggag gtcaggctgt ttttcatccg
     3181 ttttctccga tgagacgttg ttcaccggag ttcaggagct ctcactatcc tgaactccga
     3241 taagagctct ctcgccggag ttgcggtctt tctcccggct ggctacgcat ctagtctgta
     3301 tttcgatgat tttccaacag gctatagtcg atcggattat tccgcaacat ctcattggcg
     3361 attgcgatga catcctgacc ggcacccact ttaaagacaa gctgcggttc aatcgggaag
     3421 ttcacctgga tatctgcctg tttcgtaaat tgacgatcca tctgccggat caggatatcc
     3481 aaactttgga tcnnnnnnnn nngcaacatt aaaaggtgac cccagttgac ggtcaaccgc
     3541 atcataaatc atcgacacag acatgagcac actgaaacgt gaacgatcaa aaagagggat
     3601 aggcaaattc atcatcaccc caccatcgac taactgtcta ccgtctaagg ttgcagtgga
     3661 gaaaatcatc gggaacgcac aggacgccgc cacggcaatg cccaattcga tatcatgtgg
     3721 atagatgagc caatctcccg tcggacttgt gaaaaaatcg ggttcattgg tgaaaacaat
     3781 caattcactt gtatggatat caacagtcgt gatcgcgata ggcaaacgac aatcggacag
     3841 catccggcaa cctattttat caaataactg acaggccatc tgctgcagat tctgcagttc
     3901 aacgaaaccg ttattgtgtt cccgaacatg ctgaagaagc agataggcag gcctgctgaa
     3961 gacaccacga tcggcaaatt ccctttccgc atccaataac gttttctcga tttccgttgc
     4021 agtcaaccca caggccacca aagccgctat gacagctccc atagaagttc ctgctgcggc
     4081 atcgattttg acatgattct tttcgagatc atgaaggacg gcaagctgtg caaaggactt
     4141 caggcctcct ccagccaaac tgattccaaa cagccccttg cccttttccg atttttccgg
     4201 cgattccata taagttccct tccttcaaaa gtagttctct actataatta tcgcttattt
     4261 cagtagaaac tacaaaaaag aagcgtgtta tagagcgcca cctcctgcaa acgtgccctt
     4321 cgcaggaggt gacgggtata tccaggtggt ttctccggcc agattaccgc tccccgttgt
     4381 tgaagcccaa tccttatccc gttctccgac aaagccacc
//
LOCUS       DAOMUJ010000105         4397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5604_length_4397_cov_2.537080, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000105 DAOMUJ010000000
VERSION     DAOMUJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_4397_cov_2.537080"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1235)
                     /locus_tag="PLT73_03835"
     CDS             complement(165..1235)
                     /locus_tag="PLT73_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19545.1"
                     /translation="MKKLMGMAAVILFACAAIAFGINALNRAQGFSDGNTLTIYNWGD
                     YIEPELITKFEEETGYKVSYETFDSNEAMYTKVKQGGTAYDLSIPSEYMIDKMAEEDL
                     LIALDHAKIEGLENIDPRFLDLPFDEGNKYSIPYFWGTLGIIYNDKMVTEEVTSWSDL
                     WDPGYANSLLLIDGAREVMGLGLQSEGYSLNETDPIILGEIAEKLKGITPNVKAIVAD
                     EIKMYMIQEEAAIAVTFSGEASEMLWENENLHYVIPEEGSNLWFDNIVIPKTAKNIEG
                     AYAFINFMLEPENAAINAEYVGYATPNQNAIELMDPEITSDEQFYPSDEIMDKLEVYE
                     NLGAKTLGTYNDLFLEVKMFRK"
     gene            complement(1232..2083)
                     /locus_tag="PLT73_03840"
     CDS             complement(1232..2083)
                     /locus_tag="PLT73_03840"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19546.1"
                     /translation="MKGTANTKWNNAFLGLVFTILYLPIFYLIFYSFNAGGTMNDFTG
                     FTWEHYQSVFEDTRLIGIVLNTFLVALLSALIATAIGTMGAIFIYYTKKRNIKNTLLS
                     LNNVLMVSPDVIIGASLLILFTMLGFSLGFSSVLLSHIAFSIPIVVLMVLPKLYEMND
                     SMISAAQDLGASSGQVFSRILIPSITPGILSGFFMAFTYSLDDFAVTFFVTGNGFTTL
                     AVEIYSRARRGVSLEINALSALMFLFTLLLVLGYYFIQQYGHNKHAQQMKAIGKSADS
                     ERMVKRG"
     gene            complement(2080..2895)
                     /locus_tag="PLT73_03845"
     CDS             complement(2080..2895)
                     /locus_tag="PLT73_03845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19547.1"
                     /translation="MTKNSRAFYSVPYVLWLGLFVISPMLLILYQSFFDITGAFTFSN
                     YANYFTSGNYLRMTLNSFLYAFIITLVTFLVSYPTALFLSRTKHKQLWLSLIILPTWI
                     NLLLKAYAFIGLFSQSGTVNQFLGFMGISPQQILFTDFSFIFVAAYIELPFMILPIFN
                     SIEELKPSLIVASEDLGATRMQTLTRVIFPLTLSGVRSGFQAVFIPSLSLFMLTRLIG
                     GNRVITLGTAVEQHFLVTQNWGMGSTIGVILIISMIAIMSLTSDKKKKGRGSK"
     gene            complement(2907..3995)
                     /locus_tag="PLT73_03850"
     CDS             complement(2907..3995)
                     /locus_tag="PLT73_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19548.1"
                     /translation="MTDKPIITLENVKKVYDDETVLHDINFELEKGKFYTLLGPSGCG
                     KTTILRLIAGFADATEGVIKINGETVNDIPANRRKVNTVFQDYALFPHMNVFDNIAFG
                     LTIKKMPKDQIIQKVKEALKMVQLEGYENREISEMSGGQQQRVAIARAIVNEPDVLLL
                     DEPLSALDLKLRTDMQYELRELQQRLGITFIFVTHDQEEALAMSDWIFVMNKGAIVQS
                     GTPVDIYDEPIDRFVADFIGESNIVPGTMVEDYKVRFVGKEFECSDAGITPGEKIEIV
                     IRPEDLELTTVENGKLVVNIDTQLFRGVHYEIIGYDKDGNEWMIHSTKSAEIGKDVGL
                     HFGPQDIHVMRLGESEEEFDARLESYEE"
     gene            complement(4199..>4397)
                     /locus_tag="PLT73_03855"
     CDS             complement(4199..>4397)
                     /locus_tag="PLT73_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HRM19549.1"
                     /translation="RAVVMAKGKIVADTSSAFVLINPRLIQVASLKETTLFTFANQIG
                     MTDPYSFTEKFVYYDRAARLF"
BASE COUNT         1169 a         1100 c          958 g         1170 t
ORIGIN      
        1 ataccactct aattttgatg cagccccttt catttggatt tgaatttaag ctcaaaaata
       61 ccccgccaaa cccatttttg gcggggtgaa tcagttcaag tcacaatcaa atactccgtc
      121 gtgaattcat gttatttcca ctattgtata caaaagtgta tgtatcattt ccgaaacatt
      181 ttgacttcca gaaacaaatc gttatacgtc cctagggttt tggctcccag attttcgtag
      241 acttcgagtt tgtccatgat ttcgtcggac gggtagaatt gctcgtcgct ggtaatttcg
      301 ggatccatca gctcgatggc attttggttc ggtgtggcgt agccgacata ttccgcgttg
      361 atggccgcat tttcaggttc cagcatgaaa ttgatgaaag cgtacgcccc ttcgatattt
      421 ttggcggtct tggggatgac gatgttgtcg aaccagagat tggagccttc ctcagggatt
      481 acgtaatgaa gattttcatt ttcccaaagc atctcgctgg cctcaccaga gaaagtcacg
      541 gcgatggccg cttcctcctg gatcatatac atttttattt catcagcaac gatcgctttg
      601 acattcgggg tgataccttt tagtttctca gcgatttctc ccaaaatgat cgggtctgtc
      661 tcgttcaatg agtagccttc actttggagg cccagcccca tcacttctcg cgctccatcg
      721 atcaaaagca gactgttcgc atagcccgga tcccaaagat ccgaccagga agtgacttct
      781 tccgtcacca ttttgtcatt atagatgatg cccaaggttc cccagaaata agggatggag
      841 tacttattgc cttcatcgaa cggcaaatcc aggaaacgcg ggtcaatgtt ttccagacct
      901 tcgatcttcg cgtggtccag cgcaatcaga aggtcttctt cagccatctt gtcgatcatg
      961 tactctgaag ggatactcag atcataggcc gttccacctt gttttacttt tgtgtacatc
     1021 gcctcgttcg aatcgaacgt ttcataagaa actttataac ctgtctcctc ctcgaatttc
     1081 gtgatcagtt ccggttcgat ataatcaccc cagttgtaga tcgtcagcgt attcccgtcc
     1141 gaaaagcctt gcgctctgtt caaagcgttt atcccgaaag cgattgccgc acaagcaaac
     1201 aagataacag ccgccattcc cattaatttt ttcatcctct tttcaccatc cgttccgaat
     1261 cggcgctttt tccgatcgcc ttcatttgct gcgcatgctt attgtgcccg tactgttgaa
     1321 tgaaatagta gccaaggacc aaaagcagcg taaacaggaa catcagcgca ctgagggcat
     1381 tgatttccaa gctgactcca cggcgcgccc gcgaatagat ttccaccgct aaagtggtga
     1441 agccattccc cgtcacgaaa aaagtcaccg caaaatcatc caaggaatag gtaaaggcca
     1501 taaagaagcc actcaggatc cccggagtga tactagggat caggattctg ctgaaaacct
     1561 gaccgctgct ggcacccaga tcctgagccg cgctgatcat cgaatcattc atctcataaa
     1621 gtttcggcag taccatcagc acgacgatcg ggatcgaaaa cgcgatatgc gagagcagta
     1681 cagaagaaaa tcccaacgaa aagccgagca tcgtgaacaa gatcagtagg ctggcaccga
     1741 tgatgacgtc cggagaaacc atcagcacgt tgttcagact caagagcgta ttctttatat
     1801 tacgcttttt cgtgtagtat atgaagattg cacccatcgt accgattgca gtcgcgataa
     1861 gggccgacag caaggccact aaaaaggtat tcaaaacgat gccgatcaaa cgcgtatcct
     1921 caaatacgga ctggtaatgt tcccaggtga agcccgtgaa atcgttcatg gtcccgccgg
     1981 cattgaacga ataaaaaatc agataaaaga tcggcagata cagaatcgta aaaacgagtc
     2041 caagaaaggc attgttccac ttcgtgttgg ctgttccctt catttagatc ccctgccctt
     2101 cttctttttg tcgctcgtca gggacatgat ggcgatcatg ctgatgatca ggatgacgcc
     2161 gattgtggag cccatccccc agttctgggt aaccaggaaa tgctgttcca cagctgttcc
     2221 cagcgtaatt acccggtttc ccccgatcag ccgcgtcagc atgaacaacg acagcgacgg
     2281 aatgaagacg gcctgaaagc cgctgcgcac accgctcagc gtcaacggaa agatgacgcg
     2341 cgtcaaagtc tgcatccttg tcgcacccaa gtcttcgctg gctacgataa gtgacggctt
     2401 cagttcttcg atcgaattga agatcggcag gatcatgaac ggcagctcga tataagccgc
     2461 cacaaaaatg aaactgaagt ctgtgaacag gatctgttgc ggacttatcc ccatgaagcc
     2521 aaggaattga ttgaccgtcc cgctctgact gaacaaaccg atgaaggcat aggccttcag
     2581 cagcagattg atccaggtag gcagaatgat cagggacagc caaagttgct tgtgcttcgt
     2641 ccgactcagg aacaacgcgg tcggataact gaccaggaac gtaacgagtg tgatgatgaa
     2701 cgcataaagg aacgaattca aggtcatccg caaataattt cccgaagtga aataatttgc
     2761 gtagttggaa aaagtgaagg ccccggtgat atcgaaaaag gactggtaca ggatcaacaa
     2821 catcggtgaa ataacaaaca gccccaacca aagtacgtaa ggaacagaat agaacgctct
     2881 agaatttttc gtcatgtctc ttccccctac tcttcatagc tttcgagacg ggcatcgaac
     2941 tcttcttcgg attcgcccag acgcatgacg tggatgtctt gtggcccgaa atgaagcccg
     3001 acatctttgc cgatttctgc gctcttggtg gaatggatca tccactcgtt tccatccttg
     3061 tcataaccga taatctcata gtggacgccg cggaacaatt gtgtatcaat attcacgacc
     3121 agtttaccat tttcgacagt cgtcagttcc agatcttccg gacgtatgac aatttcgatt
     3181 ttttcacccg gcgtgatgcc ggcatcggaa cattcgaact ctttgcccac gaagcggact
     3241 ttataatcct caaccatcgt acccggaacg atattgcttt caccgatgaa atctgccacg
     3301 aatcggtcga tcggctcgtc gtaaatgtca acaggcgttc cgctctggac aattgcgccc
     3361 ttattcatta caaaaatcca atcgctcatg gccaacgctt cttcctgatc atgcgtgaca
     3421 aaaatgaaag tgatgccaag acgttgctgc aattccctca gttcgtactg catgtccgtg
     3481 cgcagcttca agtccaacgc cgacaagggt tcatccagga gcaacacatc cggttcgttg
     3541 actatagctc tggcgattgc aacccgctgt tgttggccac ccgacatttc actgatttca
     3601 cgattttcat agccttcaag ttggaccatt ttcaaggctt ctttcacttt ttgtatgatt
     3661 tggtccttag gcattttctt gatcgtcaat ccgaatgcaa tgttatcgaa aacattcatg
     3721 tggggaaaca aggcatagtc ctggaaaacg gtattgactt tacgtcggtt cgctggaatg
     3781 tcatttacgg tttcgccatt tatttttatg acgccttcgg tagcatccgc gaatccggct
     3841 atcaaacgca ggattgttgt ttttccacag ccggaggggc ccaataaggt atagaatttg
     3901 cccttttcaa gttcaaagtt aatatcgtgc aacacagttt catcatcgta cacttttttt
     3961 acattctcca aagtgatgat cggtttatca gtcattctct cacacctttt caatttaatt
     4021 taaggaagct taaaacggca gccttccagt gtcttctcgc actttccaac ggcattcagt
     4081 gctttcatgt taacatataa cactatcatt atataagaag taggctcatt tacaatgaaa
     4141 cctgaacaaa aaacgccaga aatgatcatt ctgacgtttg ggactaatat gaattttatt
     4201 agaacagacg cgcagcccga tcgtagtaga cgaatttttc cgtaaaggag taaggatccg
     4261 tcataccgat ttgattcgcg aaggtgaaca atgtggtctc tttcagtgaa gctacttgaa
     4321 tcagccttgg attgatgagg acaaaagcgg aactcgtatc agccacaatt ttccctttgg
     4381 ccataacgac cgctctg
//
LOCUS       DAOMUJ010000106         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5606_length_4396_cov_2.144897, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000106 DAOMUJ010000000
VERSION     DAOMUJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5606_length_4396_cov_2.144897"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>573
                     /locus_tag="PLT73_03860"
     CDS             253..>573
                     /locus_tag="PLT73_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19550.1"
                     /translation="MANYPFDKERSLLKTDESTRITMLLLNVFLIVTAVSLTISFYIL
                     ANLEINEEPVVSDIIIVPEGAPERGVKASELLEAGYSESGKIIVSPLLVDAELLHLQP
                     YGELG"
     assembly_gap    574..583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <584..898
                     /locus_tag="PLT73_03865"
     CDS             <584..898
                     /locus_tag="PLT73_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM19551.1"
                     /translation="ILVDYQATSTWTNAIYSIALMEENGFDTAIVVSSDYHMNRVKYS
                     FEKAAKGKDMTFIYVSAGGPYGLPWIETQLGRRLAQHEIFKTIGYWLGLYHFIDIGEG
                     GS"
     gene            1254..2483
                     /locus_tag="PLT73_03870"
     CDS             1254..2483
                     /locus_tag="PLT73_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM19552.1"
                     /translation="MLVEIVMENFFSFKDEATFSMVASPIGEHPESVYGKNNIAVTKL
                     ATIYGANASGKSNLLKAMKMIQEGLLLQKCSSSEWLDHIEPFHLDARTEKKDTMLEVS
                     FLLDGKLYRYGFFVNRKRVTEEFLYLEGDEQKDLFFRNATGQIAGGEWAEMFRDIILD
                     DTRLALSSLLSAPESAEHPELKLFIEWFTEMRIILDTSKIGLSISMAKMHLSKYKHNL
                     LALIRVADPSIQDIVVHELENSRNGRPELAFFVVKTRRNEAGESELHCENFRFQDTES
                     AGTVKLLALAGPIIEALEKGGLLMVDEMDTQFHTLMTRYVLELFSGDVNDNGAQMIYS
                     THDITNLSSNLFRRDQVWFVEKDTYSASHLTSLVEYRFDDEERKNSFEFYRNYLNGKY
                     GAIPFPRPLDWWVDNGK"
     gene            2473..3087
                     /locus_tag="PLT73_03875"
     CDS             2473..3087
                     /locus_tag="PLT73_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRM19553.1"
                     /translation="MENRRREHKKKFERPVGTKRPEKTFLILCEGTKTEPNYFRSFHV
                     ISAQVEIVGTAMNTMSLVNHAREVVKDRPDEYDEIWLVFDKDSFDRDIFNEAVFFCET
                     HYRQGFRAAYSNEAFEIWYLLHFELIKKSISRFHYPDMLSKRLGFFYKKNHPHMYNVL
                     LSRQPAAIQNAKKLYSQRSPSPARDNPSTTVFMLVEELNKHLRK"
     gene            3202..3456
                     /locus_tag="PLT73_03880"
     CDS             3202..3456
                     /locus_tag="PLT73_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19554.1"
                     /translation="MFMTPVLGMDFLEDKKGVVIHFVKDDTLAEEYLFETTDEAAAFF
                     RSCQNLCEEVKEEPLEVQYALIREFLDLDIGKFNYERAYY"
     gene            complement(3690..>4396)
                     /locus_tag="PLT73_03885"
     CDS             complement(3690..>4396)
                     /locus_tag="PLT73_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM19555.1"
                     /translation="YTIEKRIFSIYQNPLTASNLIIAHESGNPNNVGKNSLENEVSYM
                     LRNWQNAFVSHWVGGGGKIIQIANAGKVQWGVGPKANGYAYAQVELARTNNRSIFEQD
                     YKAYVWLLQKLALEAGIPCTLNSGASVHDKGIKTHSWVSKNVGGTDHTDPDGYLASWG
                     ISQARFRQDIEAGLSALPPLASAPGTFLLHRVVKGDTLWGLSRKYGTTPATLKQLNQL
                     SGNLILIGQQLKVRQY"
BASE COUNT         1129 a         1009 c         1155 g         1093 t
ORIGIN      
        1 tctttagcaa acaggcgaca gatggcttcg cccatacccg aagcgcctcc agtcacaaca
       61 gcgactttgt tcatcagttt tatgtcgttc tcctccttat gccttgctgg tctaattata
      121 tcacgacaac gctgacaagc ttgaggtgac gatccactca aaaataaaaa atataaaaag
      181 cgatggcaaa tttccacatt tcagataaaa tagagtgata ataaggtgag gaaagggtgt
      241 ggagagcaga agatggcaaa ttatccattc gacaaggagc gcagcctcct gaagacggac
      301 gaatcgacca gaataacgat gttgctgctg aatgtatttc tgatcgtgac tgccgtatct
      361 ttgacaatca gcttctatat cctcgccaat ctggaaatca atgaggaacc ggtcgtttcc
      421 gacatcatca tcgtacctga aggcgcgccg gaacgaggag ttaaggcctc agaactgttg
      481 gaagcaggtt attccgaatc cggcaaaatc atcgtttctc cgctgcttgt ggatgccgaa
      541 ctattgcatc tgcagcccta cggcgaactt gggnnnnnnn nnnatactgg tggattacca
      601 agcgaccagc acttggacca atgccatata cagtatcgcg ttgatggaag aaaatggttt
      661 cgacacagcg attgttgtgt cgagcgatta tcacatgaac cgggtcaagt acagcttcga
      721 aaaagctgca aaaggaaagg atatgacatt catctatgtg tcagctggcg gtccgtacgg
      781 cttgccatgg atcgaaaccc agctcgggag acgcctagcg cagcatgaaa tctttaaaac
      841 aatcggttat tggctaggtt tgtatcattt catcgatatc ggcgaaggcg gctcttaatg
      901 gggagtgcgt ctttttttat ggttcggggt acctgatttc cggtaagatg gtggctcgcc
      961 ggagattggg tgtggattca ccagctcaat tccggcgagg aaggcttggg cggagaacaa
     1021 cgcataatca tagttgtttc ctccggcgag gcaaacgttc cccggagctg gagagcccgg
     1081 atttgttcgc cgcagtgtga tattcgctga tgatgcaaag atccacaaat tccctacaaa
     1141 tccgcgcttt tgtatccgtt tgcaataatg ttatccgctt ttgcattctt gacggttatt
     1201 cttagaagaa aagccgccag ggtgctatac tttttggtat aggaggtgtc gagatgcttg
     1261 tagaaatagt gatggagaat tttttttcct ttaaagatga ggctaccttt tcgatggtcg
     1321 ccagcccgat cggcgaacac ccggagtccg tctatggcaa aaacaatatc gctgtgacaa
     1381 aattggcgac gatctatggg gcgaatgcca gcgggaaaag caatctgctg aaggccatga
     1441 agatgatcca ggaaggccta ctgttgcaga agtgctcatc cagtgaatgg ctcgaccata
     1501 tcgaaccgtt ccatctggat gcgcgcaccg agaaaaagga tacgatgttg gaagtaagtt
     1561 ttcttttgga cgggaagctt tatcgctatg gttttttcgt caaccgcaaa cgggttaccg
     1621 aagaattcct ttacctggaa ggcgacgagc agaaggatct gtttttccgg aatgcgaccg
     1681 gacagatcgc cggtggggaa tgggctgaaa tgttccgcga catcattttg gacgatacgc
     1741 gtttggcctt gtcttcgttg ctttccgctc ctgaatcagc tgaacatccg gaactgaaac
     1801 tgttcataga atggttcacg gagatgcgca tcatcctcga cacttcgaaa atcggattgt
     1861 cgatttcgat ggcgaagatg cacctgtcaa aatacaagca taatctcttg gccttgattc
     1921 gggtggccga tccgtccatt caggatattg ttgttcacga attggaaaat tcgcgcaatg
     1981 ggaggccgga acttgccttt tttgtcgtca agacacggcg caatgaagcc ggggaatcgg
     2041 agctccattg cgaaaatttc cgtttccagg ataccgaatc ggcaggtacg gtcaagcttt
     2101 tggctttggc cggtccgatc atcgaggcgc tcgaaaaagg tggactgttg atggtcgatg
     2161 aaatggatac gcaattccac actttgatga cgcgctacgt gctggaactt ttttccggcg
     2221 acgtcaatga taatggtgcg cagatgatat actcgacgca cgatatcacg aacctttcca
     2281 gcaacctttt ccgcagggat caagtctggt tcgtggaaaa ggacacgtac agcgccagcc
     2341 acctgacttc cttggttgaa tatcgttttg atgacgagga aaggaagaat agttttgaat
     2401 tttaccgcaa ctacctgaac ggcaaatatg gggcaatccc atttcctcgg ccgttggatt
     2461 ggtgggtcga caatggaaaa tagaagaaga gaacacaaga aaaaatttga acggccggtc
     2521 ggaacgaaaa ggccggaaaa aactttcctg attctttgcg aaggcactaa aaccgaaccg
     2581 aactacttcc gcagttttca cgtcatctcg gcgcaggtcg aaatcgttgg tacagcgatg
     2641 aatacgatgt ctttagtcaa ccatgcgcga gaggtcgtca aggatcgacc ggacgaatat
     2701 gatgaaattt ggctggtatt cgataaggat tcatttgacc gggatatctt caatgaagcc
     2761 gtctttttct gtgaaacgca ttaccggcaa ggcttccgtg ccgcctacag caacgaagct
     2821 ttcgaaatct ggtatctgct gcattttgag ctgatcaaaa aatcgatttc acgtttccat
     2881 tatcctgata tgctgtcgaa acgactggga tttttctaca aaaaaaatca tccgcatatg
     2941 tataacgtac tattatcaag gcagccggca gccatccaga acgccaagaa actctacagt
     3001 cagcgctcgc cgtcgcctgc ccgcgacaat ccatccacga ccgttttcat gctggtcgag
     3061 gagctgaaca agcatctgag gaaataattt aaaaaagttc aaaaataaaa aaagctggaa
     3121 cgattttttg cgaataatac cagtgtaggt cttatcaagc agctaaggcc acttaaaaag
     3181 acaaaagggg attgattgca tatgttcatg acaccggtat tgggaatgga tttcttggag
     3241 gataagaaag gggtcgtcat tcattttgtc aaagacgaca cattagcgga ggagtatctg
     3301 ttcgaaacaa ccgatgaggc agccgcgttt ttcagaagct gccaaaactt gtgcgaggag
     3361 gtcaaagagg aaccgctaga ggtccaatat gccctcatca gagagttttt ggatctggac
     3421 atcggaaaat tcaactacga acgcgcttat tattgataaa cataagaaaa agaaggccag
     3481 ccccggaatg attcagggct ggccttcttt ttggcgccgg aattggcgcc ctcagctccg
     3541 gcgaggggtc ggatcgccgg aggtcaccgg taatgatggg cttcatcttc gacgaagcct
     3601 gcctcatcgg agcaggaatc aaaaatgtga actgaactgc ggcgaagcct gcgttccccg
     3661 gagcagacca ttcccggcgg taggcaggct cagtactggc gcaccttcaa ttgctgtcca
     3721 atcaagatca gattgcccga cagctggttc agctgcttta gcgtagctgg ggtcgttcca
     3781 tatttgcgcg acaagcccca gagcgtgtcg cctttcacga cacggtgcag caggaacgtg
     3841 ccgggtgcgc tggcaagggg aggcagcgcg gagaggccgg cctcgatatc ctgacgaaag
     3901 cgggcctgcg atatacccca actcgcgagg tagccgtccg gatcggtatg atcggtcccg
     3961 ccgacatttt tggaaaccca ggaatgggtt ttgatgcctt tgtcatgtac gctcgctccg
     4021 ctgttcagcg tgcagggaat gcctgcttcc agcgctaatt tctgcagcag ccatacgtag
     4081 gctttgtagt cctgttcaaa gatggagcga ttgttcgtcc gcgccagctc gacttgggca
     4141 taggcataac cgttcgcttt cgggccgaca ccccattgga ctttgccggc gttggcgatc
     4201 tggattattt tcccgcctcc gccgacccaa tgggaaacga aggcgttttg ccagttgcgc
     4261 agcatgtaac tgacttcgtt ctcgaggcta tttttgccga cgttgttcgg attgccggac
     4321 tcgtgggcga tgatcaggtt gcttgcggtg agtggattct ggtagatgga aaagatgcgt
     4381 ttttcgatcg tgtagt
//
LOCUS       DAOMUJ010000107         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5630_length_4385_cov_3.289838, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000107 DAOMUJ010000000
VERSION     DAOMUJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_4385_cov_3.289838"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1061
                     /locus_tag="PLT73_03890"
     CDS             375..1061
                     /locus_tag="PLT73_03890"
                     /inference="COORDINATES: protein motif:HMM:NF018296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HRM19556.1"
                     /translation="MEETKSVAQMKVENKALWQSLTKRNEQYMMGLDRTLRAANLEEE
                     RREEIYNKMMRELLEAQKTGKTARQLYGTVSECASNILVNPSDNEVKVRSSDWLIALD
                     GGLLLGSLFAMISGISLLTNSGEDVIGMGLISLILNFVVGGIAMLVISKYTPNPDAPK
                     GEKGFGKYIFATTGAMLLWMLIMTVSMMLIPASINIALPAWIYLGIAGIGFAAKVYLK
                     KKYHIMGGIL"
     gene            1336..2820
                     /gene="guaB"
                     /locus_tag="PLT73_03895"
     CDS             1336..2820
                     /gene="guaB"
                     /locus_tag="PLT73_03895"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRM19557.1"
                     /translation="MSNWETKFAREGFTFDDVLLVPAESHVLPNDVDLSIDLAKNLHL
                     NIPIISASMDTVTDSAMAIAMARQGGLGVIHKNMTIEAQAEEVHKVKRSESGVIIDPF
                     FLTPDHLVSEAENLMSKYRISGVPVVNDMEDRKLVGILTNRDLRFIADYQVPIGDVMT
                     KEHLVTAPVGTSLKDAESILQKYKIEKLPLVDDSGRLAGLITIKDIEKVIEFPNSAKD
                     AHGRLIVAAAVGVTSDTFERAQALLDAGADAIVIDTAHGHSAGVIRKIKEIRDQFPDA
                     TLIAGNVATAEGTRALFDVGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAASVAR
                     EYGKAIIADGGIKYSGDIVKAMAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLAAMEKGSSDRYFQGGVNEAKKMVPEGIEGRVAYKGAASDIIFQLLGGVRSGMGY
                     VGAGNLQELRENAQFIKMSGAGLIESHPHDIQITKEAPNYSIHR"
     gene            3001..3693
                     /locus_tag="PLT73_03900"
     CDS             3001..3693
                     /locus_tag="PLT73_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM19558.1"
                     /translation="MSKILIVDDDREIVDLLSIYCKNEGYDPIKAYDGVDAFHKLEEN
                     ADIQLIILDVMMPKKDGISVVRELREQNNPIPILMLSAKSADMDKIQGLTTGADDYVS
                     KPFNPLEVMARIKSLLRRTTMQADAAEVDSIEIGPLIIKKDSHEVKTLNGKSIQLTAL
                     EFGILHLLASHPNRVFSADEIFERVWQQESIVSAKTVMVHVSHLRDKIEEATGGEKVI
                     QTVWGVGYKIGE"
     gene            3743..>4385
                     /locus_tag="PLT73_03905"
     CDS             3743..>4385
                     /locus_tag="PLT73_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRM19559.1"
                     /translation="MFNKLKKKELSELVFEGALTLGIVYLLYIGMELMFNRLVANPPM
                     ILREVPSLRRFVMVVDAFLTIYDDVFQWIAAIFAAIFTGWRLVRRYKQMQLTHILDEL
                     HYIAAGNFDHRIDANNAGSYVDVVDSINTLVESTVQAMEEERKIEQSKDELITNVSHD
                     IRTPLTSIIGYLGLIEDKQFTSEEDALRYIHIAYTKALQMKVLVDDLFEYTKVR"
BASE COUNT         1250 a          885 c         1161 g         1089 t
ORIGIN      
        1 gtcagcttag gcggaatccc ctgcatttca gagttcaact aaaggatacg atgaaatgcg
       61 ggaggggatg agcactaaga ggatattacg agaacggccg cagtttggcc gtgacgtaat
      121 gtcagcttag gcggaatccc ctgcatttca gagttcaact aaaggatacg atgaatcccg
      181 ggtagaaacc gagcactaag gggccaagca caaagcggat gctttttgtc cgtgtgtgtt
      241 tggcaactta ggcggaagtt tctggaattc agagttcaac taagagcgtg tgatgaatct
      301 ctctttattt cagattcagg gattcacaac acgtttgaca agggtgccgc atgtaatcaa
      361 gggaggattt atcattggaa gaaacgaaga gtgttgcaca aatgaaagtc gaaaacaaag
      421 ctttatggca gagtttgaca aaaagaaatg aacaatacat gatggggctg gacaggacct
      481 tgagagctgc aaacctggaa gaggaacgca gagaggaaat ctacaataaa atgatgcggg
      541 agttgctgga agcccaaaaa accggtaaaa ccgcccgcca attatatggc acagtatctg
      601 agtgcgcctc gaatatcctg gtgaatccgt cagacaacga agtgaaagta cgttcgtctg
      661 attggctgat cgctttggat ggcggtttat tgctagggtc tcttttcgcg atgatttcgg
      721 ggatttcctt gctgaccaac agtggcgaag atgtcatcgg aatgggtctg atcagcttga
      781 ttctgaactt tgttgtgggc ggtatcgcca tgctcgtcat ctctaaatac acgccgaatc
      841 cagacgctcc aaaaggcgaa aaaggctttg ggaaatatat ctttgcgacg acaggtgcta
      901 tgctgctttg gatgctcatc atgactgttt ccatgatgct tattccagca agcattaata
      961 tcgcgctacc tgcgtggata taccttggaa tcgctgggat aggttttgct gcaaaagtat
     1021 atttgaagaa aaaatatcat atcatgggcg gcatattgta gcccgtcaat tggtggaggc
     1081 cgagatgcaa aggaatactt gtatctcggc tattttcgta tattgataga gaaaaaactg
     1141 aaaatgaatc aaaggatata accgaacgcg ttaattccga acgttatgtc agatttaaat
     1201 aaatagcatt cgataaaaag cgctgctata tgagaattgt atgataaagg gggatttgcg
     1261 cttgacgtgg gttgttgagg gtggtatttt agatgataaa aaatcagcat aaataaaaag
     1321 gagtgttcat cgaaaatgtc aaattgggaa acaaaattcg ctagagaagg ttttactttt
     1381 gatgatgtat tattagtccc ggctgaaagc catgtattac ctaacgatgt ggatttatcg
     1441 attgatttag ctaaaaattt acacttgaat attcctatca taagcgcaag tatggacaca
     1501 gttaccgatt cggcgatggc aatcgcaatg gcccgccaag gcgggttggg cgtcatccat
     1561 aaaaacatga ccatcgaagc gcaggctgaa gaagtgcata aagtaaaacg ttccgaaagt
     1621 ggcgtcatca tcgacccatt cttcctgacc ccggatcact tggtctctga agcagagaat
     1681 ctcatgagta aataccgtat cagcggtgtg cctgttgtca atgatatgga agaccgcaag
     1741 ttggtaggga ttttgacgaa ccgcgatctg cgattcattg cagactacca agttccgatc
     1801 ggcgatgtga tgacaaaaga acacttggtt accgctcctg tagggacttc tttgaaggat
     1861 gccgaatcca ttctgcaaaa atataaaatc gaaaaattac ctctagttga tgacagtggc
     1921 cgtctggctg gtctgatcac aatcaaggat atcgaaaaag taattgagtt tcccaactcc
     1981 gcaaaagatg cacacggccg tttgatcgtt gcagctgcag taggtgtgac aagcgataca
     2041 ttcgaacgtg cccaagcctt attggatgcc ggtgcagatg caatcgtcat cgacacagcg
     2101 catggccata gcgccggcgt tatccgcaaa attaaagaaa tccgcgatca gttccctgac
     2161 gcaaccttga ttgccggaaa tgtagcgact gcagaaggaa cccgcgcttt gtttgacgtc
     2221 ggagtcgatg tagtcaaagt cggaatcgga ccgggctcga tctgtacgac ccgtgtcgta
     2281 gccggtgttg gtgttccgca aatcacagcc atctatgatg ctgcaagtgt ggcgagagaa
     2341 tacggcaaag caattatcgc tgacggcgga atcaagtatt cgggagacat cgtcaaagcg
     2401 atggctgccg gtggacatgc cgtcatgttg ggcagtatgt tggctggtac ggatgagtct
     2461 ccgggcgaat tcgaaatctt ccaaggccgt cgtttcaaga cataccgtgg catgggcagt
     2521 ctggcggcaa tggaaaaagg ctccagcgac cgctacttcc aaggcggggt gaatgaagcg
     2581 aagaaaatgg ttccggaagg tatcgaaggc cgtgtcgctt ataaaggggc agccagcgat
     2641 atcatcttcc aactgctcgg cggtgtccgt tccggtatgg gttatgttgg tgccggcaat
     2701 ctgcaggagc ttcgcgaaaa tgcacaattc atcaagatgt ccggtgccgg attgatcgaa
     2761 tcccatccgc atgacatcca aatcactaaa gaagcaccaa actactccat ccatagataa
     2821 acaggcaaga aaccaaaacg cccgggacac tgtttccggg cgttttttct tatgttcgca
     2881 gtttgctggg gctttctgat ataattaaaa tggaatttct ttatgcttcc ttaagcaaaa
     2941 atttagaaca ctttggtacg atggggaaaa atacatattt tggggaaagg gttgtccgat
     3001 atgagtaaaa ttttgatcgt cgatgacgac agggaaatcg tggatttgtt gagcatttat
     3061 tgcaaaaatg aaggatacga tccgataaaa gcctatgacg gcgtggacgc tttccacaag
     3121 cttgaggaga atgcagatat ccaactgatc attttggatg tgatgatgcc gaagaaggat
     3181 ggcatctcgg tagtgaggga gctgcgggaa cagaacaatc cgatcccgat tctgatgttg
     3241 agcgcaaaat ccgccgatat ggataaaatc caagggttga cgacaggtgc ggacgactat
     3301 gtttccaagc cgttcaatcc actggaagtg atggctcgca tcaaatcgct cttgcgcagg
     3361 accactatgc aggcagatgc agcagaggtg gattcgatcg aaataggtcc gctgatcatc
     3421 aagaaagatt cccatgaggt aaagacgctg aacgggaaaa gtatccagtt gaccgcgctg
     3481 gaatttggta ttttgcacct attggcgagc catccgaatc gcgtcttcag tgccgatgaa
     3541 atttttgaac gtgtttggca acaagagagt atcgtatcgg ccaagaccgt tatggtgcat
     3601 gtgagtcatc tccgcgataa aattgaagag gctacagggg gcgagaaggt catccagacc
     3661 gtttggggtg taggctataa gatcggcgaa taagcaagcc tgtaggcaca ttggcaaaaa
     3721 aaacagctgg gaggctgcag ctttgtttaa caagttgaaa aaaaaagaac tgagtgaact
     3781 cgtttttgaa ggggcgttga cgctgggtat cgtctatctg ctctacatcg ggatggagct
     3841 gatgttcaac cgactggtcg caaaccctcc gatgattttg agggaagttc cctccttgag
     3901 gcgattcgtg atggtagtcg atgcgttctt gactatttac gatgatgttt ttcaatggat
     3961 agcggccatt tttgcggcca tcttcactgg ttggcgtttg gtcaggagat acaagcagat
     4021 gcagttgacg catatcctgg acgaactgca ttacatcgcc gcggggaact ttgaccatcg
     4081 gatcgacgca aacaatgccg gaagctatgt tgatgttgtg gacagtatca atacgctcgt
     4141 ggaaagtacg gtccaagcga tggaagagga acgaaagatt gaacagtcga aggatgagtt
     4201 gatcacgaac gtcagtcacg acatccgtac tcctttgaca tccatcatcg gatacttggg
     4261 attgatagag gataagcaat tcacaagtga agaggatgct ttgcgatata tccatatcgc
     4321 ttacacaaaa gcgctgcaga tgaaggtgct tgtcgatgat ctcttcgaat acacgaaagt
     4381 gcgac
//
LOCUS       DAOMUJ010000108         4373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5657_length_4373_cov_1.984484, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000108 DAOMUJ010000000
VERSION     DAOMUJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_4373_cov_1.984484"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLT73_03910"
     CDS             complement(<1..857)
                     /locus_tag="PLT73_03910"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRM19560.1"
                     /translation="MTDLEDYRKELDALDRELVALFEKRMQLSQSIGNYKRAHDLPIF
                     DESREQRVVEQNLTHLKSEEFAVQTHHFLDTIMDLSKEVQKDIRAAANDYEQIFAIHE
                     PKQQAQIGYFGETGSFCEEAMLDYFKEAPVDPKNYHTFESLFIGIQNGEIDYGVLPVE
                     NSSTGAISQVLDLLYKYGLSIVGEQYVKIEQHLIGLEETSLDQIEEVYSHPQGFQQST
                     DYFKDHEQWRQIPFHSTSDSAKMVKDSGDVHKGAIASRRAAAIHGLSILAESIQNQSE
                     NTTRFIIVG"
     gene            complement(881..1978)
                     /gene="aroC"
                     /locus_tag="PLT73_03915"
     CDS             complement(881..1978)
                     /gene="aroC"
                     /locus_tag="PLT73_03915"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRM19561.1"
                     /translation="MSGVFGNKLKVSIFGESHGSAIGVTIDGLPPGHEIDMDKVLEEM
                     KRRAPGQGALTTPRKEKDQPEILSGYFNDMTTGSPLAAIIRNSDTRSKDYSQMKKLMR
                     PGQADYPGYVRYDGFNDYRGSGHFSGRITAPLVFAGAIAKQLLEKQGITIGGHVKSVA
                     KIQDDSFLNTEVTPEMLKSFAGKELPLLNESLEEEMRNLIREAKASGDSVGGTAEICV
                     LGIPAGVGNPFFDSVESVLAHILFSVPAIKGLEFGSGFGIAEMLGSEANDSYYYDGDQ
                     IKTRTNHNGGILGGITDGMPIIYKVAIKPPASITKVQQTINIEDKTAAELSVEGRHDP
                     IIVPRAIPVLEAVTAIAILDLLLDSKFYKYD"
     gene            complement(1975..3270)
                     /gene="aroA"
                     /locus_tag="PLT73_03920"
     CDS             complement(1975..3270)
                     /gene="aroA"
                     /locus_tag="PLT73_03920"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRM19562.1"
                     /translation="MDLTIQPHKLSGTVTVPPSKSLAHRAVICAALSDGTCKIDNIAL
                     SDDIIATIEAMKAFGAIIEQEGSALTITGAGQYVTEAKVAESATTAEHLIDCNESGST
                     LRFVVPISTLFQGASRFVGRGNLGKRPLTIFYDIFEEQGITYKPTEGELDLVVDGNLK
                     PGHFSFPGNVSSQFITGLLFTLPLLDGDSVIKITTPLESIGYIDLTLDVMSAFGVTIE
                     NVGHQLFRISGGQSYKATDYRVEGDYSQAAFFLCADALGNDLLVDDLSMDSLQGDRAV
                     VSILEAMGVSFEKQSNGLIGTATNGLHGTLIDASQCPDIIPVVALTACLSEGKTEIIN
                     AGRLRIKECDRLQATASELKKLGADIEELPEGLLIHGVKSLKGKETVWSYKDHRIAMM
                     LAIAATVCEQPITITDAECVSKSYPDFWEDYKAIGGKIQ"
     gene            complement(3331..>4373)
                     /gene="aroB"
                     /locus_tag="PLT73_03925"
     CDS             complement(3331..>4373)
                     /gene="aroB"
                     /locus_tag="PLT73_03925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRM19563.1"
                     /translation="SYTILIENGLRHRLPQEIKKIYSGKKIAVITDHNVNAHYGAQLE
                     EGLQAVGYETLVVVLPAGEQTKDFQMLPQIYDQLLDFQLNRSELIIALGGGVIGDLAG
                     FAAASFLRGVPFIQIPTSLLAQVDSSIGGKVGVDLARGKNLVGAFYQPKKVFIDPEML
                     NTLSDHFFRDGLGEVIKYGCIKDAEFFSFLQTLHTREELMAHIEKILYTCCDIKRRVV
                     EEDEKDTGERMLLNFGHTIAHALETYTHYSKYSHGEAVAIGMVAITRLSEKKGFTKPG
                     TAAAIKALAKQVGLPTELNIGEFDLDSLLAIMTLDKKNLDNHLKVILLKEIGNSYVQN
                     ETIAFFDGLENM"
BASE COUNT          968 a         1172 c         1042 g         1191 t
ORIGIN      
        1 ttgccgacga tgatgaagcg cgttgtattt tcactttgat tctggatgct ctccgccaaa
       61 atggagagcc cgtggatcgc tgcggctctt ctgctggcga tggcaccctt gtgcacatcg
      121 ccggaatcct tcaccatttt ggcgctgtcg gatgtgctgt ggaatggaat ctgacgccac
      181 tgttcatggt ccttgaaata atcggttgat tgctggaagc cttgtgggtg cgaatagact
      241 tcctctatct gatcaaggct ggtctcttcc aggccgatca gatgctgttc gattttgacg
      301 tattgctcgc ccactatgga aagtccatat ttgtacaaca ggtcgagcac ttgtgaaatc
      361 gcaccagtag aggaattttc cacaggcaga acaccataat cgatttctcc gttctggata
      421 cctataaata atgattcgaa ggtatggtag tttttcggat ccacaggcgc ttctttaaaa
      481 taatccagca tcgcttcttc gcagaaggat cccgtttccc cgaaataacc gatctgcgcc
      541 tgctgctttg gttcgtggat tgcaaaaatc tgctcataat cgttcgcggc tgcccggata
      601 tccttctgga cctccttcga aagatccatg atcgtatcca ggaaatgatg ggtctgcacc
      661 gcaaactcct cactcttcag atgggtaaga ttctgttcca ctacacgctg ctcccggctt
      721 tcatcaaaaa tgggcaaatc gtgggcccgc ttgtaattac cgatagactg ggaaagctgc
      781 atccgtttct cgaacagcgc cactagctcc cggtcaagag catccaactc tttacgatag
      841 tcttctaaat ccgtcatact cgccttccac tccgttcatc ctagtcgtat ttgtagaatt
      901 ttgaatccaa cagcagatcc aggattgcga tcgccgtcac ggcctccaat acagggatgg
      961 cgcgcggtac gatgattgga tcgtgtctgc cctcaaccga taattcagca gccgttttgt
     1021 cttcgatatt gatggtttgc tgcacctttg tgatggatgc tggcggcttg atggcaactt
     1081 tgtagatgat cggcatgccg tccgtgatgc cccccaaaat accaccgtta tggttcgtac
     1141 gggtcttgat ctgatcgccg tcatagtagt aggaatcatt cgcttccgat cccagcatct
     1201 ctgcgatacc gaagccgctt ccgaactcca atcctttgat ggcaggcacg gagaatagaa
     1261 tgtgcgccag aacggactca acggaatcaa agaatgggtt gccgactccg gctgggatgc
     1321 ccaatacgca gatttccgca gtaccgccga cggaatcgcc gctggctttc gcttcacgga
     1381 tcagattacg catctcttct tcaagcgact cgttcaataa aggcagttct ttaccggcaa
     1441 agctcttcag catttcaggt gtgacttccg tattaaggaa gctgtcgtcc tggattttgg
     1501 caacgctctt gacgtggccg ccgatggtga tgccttgttt ttcaagcaat tgcttcgcaa
     1561 tcgcaccagc aaatactaac ggtgccgtga ttcggcccga gaaatggccg ctgccgcggt
     1621 aatcattgaa gccgtcgtaa cggacatatc ctggatagtc ggcctgtccg ggacgcatca
     1681 acttcttcat ctggctgtag tccttggagc gcgtatcgct gttgcggatg atcgccgcaa
     1741 gcgggctgcc tgtcgtcatg tcgttgaagt aaccgctcaa gatttcaggc tgatcttttt
     1801 ctttccttgg cgtcgtcaaa gctccttggc ctggcgctct ccgtttcatt tcttccagaa
     1861 ctttatccat atctatttca tggcctggcg gcagaccgtc gatggtgaca ccgattgcgg
     1921 acccatgtga ttctccgaaa atggatacct ttaatttatt cccaaataca ccactcattg
     1981 tatcttgcct cctattgctt tatagtcttc ccaaaaatcc ggatacgatt ttgaaacgca
     2041 ctcggcatcc gtgatcgtga tcggctgttc gcacactgtc gcagcgatcg ccaacatcat
     2101 ggcgatgcga tggtctttgt agctccagac cgtctctttg cccttcagcg atttaacgcc
     2161 atggatcagc aggccttccg gcaactcttc gatgtcagcg cccagtttct tcaattcgct
     2221 ggctgttgct tgcagacgat cacattcttt gatgcgcagt ctgccggcat tgatgatttc
     2281 cgttttgcct tcgctcagac atgctgtcaa cgcgacgacc ggaatgatgt cggggcattg
     2341 cgacgcatcg atcagcgtac cgtgcagccc atttgtcgcg gtgccgatca ggccattgct
     2401 ttgcttttcg aaagaaacgc ccatcgcttc cagaatggag acgacagcgc gatcgccctg
     2461 cagcgaatcc atcgagagat cgtccaccaa caagtcgttg cccaatgcat cagcgcaaag
     2521 gaagaaggcc gcttgggaat aatccccttc gacgcggtaa tccgtcgctt tgtaggactg
     2581 tcctcccgaa atccggaaga gttgatggcc aacgttttcg atggtcacac cgaaagcact
     2641 catgacatcc agcgttaaat cgatatagcc gattgattca agcggcgtcg tgattttgat
     2701 gaccgagtct ccatccaaca acggtaaggt gaacaagaga cccgttatga attgtgagct
     2761 gacattccca ggaaatgaga aatgacccgg tttcaggttg ccgtccacta ccaaatcaag
     2821 ttcgccttca gtcggtttgt aagtgatgcc ttgttcctcg aaaatatcgt agaagatcgt
     2881 caatggacgt ttgcccagat tccctctgcc gacgaagcgg ctcgcgcctt ggaaaagcgt
     2941 tgaaatcggc acaacaaaac gcagcgtcga accggattca ttgcagtcga tcaggtgctc
     3001 agcggtcgtt gcactctcag caacttttgc ttctgtgacg tattggcccg cgccggtgat
     3061 cgtcaaggcg ctgccttcct gctcaatgat tgccccgaat gccttcatgg cctctatagt
     3121 tgcgatgatg tcatccgaaa gcgcgatatt gtcgattttg caggtgccgt cggaaagcgc
     3181 ggcacagatg acagctcggt gcgccaaact ctttgaaggc gggacagtca ctgttcccga
     3241 tagcttatgt ggctgtatcg tcaaatccat ggtaatcctc ctgtttgttt cttcgatttt
     3301 atttctgctg tttcttctat agtattcgtt ctacatgttc tccagaccat caaagaaggc
     3361 gatggtttcg ttctgtacgt agctgtttcc gatttccttc aacaggatca ctttcagatg
     3421 gttgtccaaa ttctttttgt ccaatgtcat gatcgccaac aagctgtcca agtcgaattc
     3481 ccctatgttg agctccgtag gcaatccgac ttgtttcgcc aatgctttga tggcagctgc
     3541 cgttcccggt ttggtgaagc cttttttctc ggaaagacgt gtgatggcga ccatgccgat
     3601 cgcgaccgct tctccgtgcg agtatttcga ataatgggtg tacgtttcca aagcatgggc
     3661 aattgtgtgg ccaaagttca gcagcatccg ttcgccggta tccttttcat cctcttccac
     3721 gactctgcgc ttgatgtcgc agcacgtgta gaggattttt tcgatgtgcg ccatcaactc
     3781 ttcacgggta tgcagtgtct gcaagaaact gaagaattca gcgtccttga tgcagccgta
     3841 cttgatgact tcgcctagac cgtcacggaa aaagtgatcc gacaacgtgt tcagcatttc
     3901 cggatcgatg aatactttct tcggttgata gaaagctccg accagattct tacctcttgc
     3961 caagtctacg ccgaccttgc ctccgatgct gctgtccact tgagccaata acgaagtcgg
     4021 aatctggatg aatgggacac ctcgcaggaa acttgcagct gcaaatcccg caagatcccc
     4081 gatgacgccg ccacccaagg caataatcag ttcactccga ttcagttgaa aatccaataa
     4141 ttggtcataa atctgcggca gcatctgaaa gtccttcgtc tgctcaccag ctggcaagac
     4201 aaccacaagc gtctcgtaac cgacagcttg aagcccttcc tccaactgtg ctccgtaatg
     4261 cgcattgacg ttgtgatccg tgatgacggc aatcttctta ccgctgtata tcttcttgat
     4321 ttcttgcgga agacggtggc gcaagccatt ctcaatcaag atcgtatagc tgc
//
LOCUS       DAOMUJ010000109         4317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5749_length_4317_cov_2.444158, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000109 DAOMUJ010000000
VERSION     DAOMUJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_4317_cov_2.444158"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1074)
                     /gene="dapA"
                     /locus_tag="PLT73_03930"
     CDS             complement(187..1074)
                     /gene="dapA"
                     /locus_tag="PLT73_03930"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRM19564.1"
                     /translation="MAIFEGSGVAIVTPFKDTENQEINYEVLEELIEFQIANGTDSII
                     IAGTTGEASTLTDEEQLDLIRFTVEKVNGRVPVIGGAGSNDTRHGVGLTRAVEATGVD
                     AILSVTPYYNKTSQKGLIEHYKAIANSVKVPIVLYNVPGRTGQNITPESLVELAKIDN
                     IVSLKDATGNFGQAVDNLRLLGDRLDIYSGNDDTIIPLMSLGAKGVISVLANIAPKAT
                     AEMTHAWLDGDIKKAAAMQAEYKELIDCLFVEPNPIPVKAGMQLLGFNVGPMRLPLTD
                     ADQAVIDRLAAAMKKVGLI"
     regulatory      complement(1215..1361)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1652..2032
                     /locus_tag="PLT73_03935"
     CDS             1652..2032
                     /locus_tag="PLT73_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014571892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19565.1"
                     /translation="MKIEHVAVWVDDLELMRDFYVRFFDGKANKLYRNPEKDFQSYFI
                     TFREGARLELMKRMNVTERYPFDMLGWAHLAVSVGSKQNVDFFTDRLIKAGYTCKSQP
                     RVTGDGYYESMFLDPEKNVIEITI"
     gene            2067..2729
                     /locus_tag="PLT73_03940"
     CDS             2067..2729
                     /locus_tag="PLT73_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HRM19566.1"
                     /translation="MMKRNHLFADQTKAGRDMRKKRTRFLIAGAIGTSIGLTAGFLLR
                     WELTPLIGWDSAAIILLYLLWKDFHPHDGTETERIAQEEDIQYSTIDFFVLLACLISI
                     GAVALMLTTQERSFETIGFGLFSIVLSWTTVHGLYMLRYAELYYRGVNGGVVFEGTEK
                     PNFWDFAYLAFTIGMTYQVSDTTFTTPEFRKAALGHALLSFIFGTAIIATTINFVASL
                     SR"
     gene            2745..4109
                     /locus_tag="PLT73_03945"
     CDS             2745..4109
                     /locus_tag="PLT73_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM19567.1"
                     /translation="MFFKSFFDPHIAQFSYLVGCQKTGEAIIIDPLRALDDYIQAAED
                     EGLVITAATETHIHADYASGLQESGRRLGAKLYVSDLGGDDWRYQNLPEGSVLLQDGD
                     IISVGKVKLEVLHTPGHTPESVSFVLTDIGGGSDIPMGVFTGDFIFVGDVGRPDLLEE
                     AAHMQGTTEIGAKAMFRSLQKMADYPDHLQIWPGHGAGSACGKSLGAVSMTTLGYEKY
                     NNWAFQYDEETSFIEALTQDQPEPPSYFAQMKKINKRDSGAYVPYPIFPLQQAGPNDR
                     MIDLRAKEIYQAGHIERTLNIPLNKKFLTYVGWFLDYDEQVTLIGTKEDAETATRQLQ
                     LIGFDQVRGYLDAGQIAGGKMTETITAAAFIALRQEKDLQILDVRSQSEWNEGHLSDA
                     KRVLLGKLLEAPLPFKRDEPLYVHCQSGVRSAVAIGALEERGFKKIINILGGYTAIEN
                     SLNG"
BASE COUNT         1106 a          981 c         1127 g         1103 t
ORIGIN      
        1 ggtcatggaa ggggatttcc atgaagttga gggagttcta tgtgaagcga gccatcggtt
       61 ttgtcattgt tatgcttctt gtactgatta ttttattggt aaataaatat ctgctgcatc
      121 ttttctgaca aatacaaaac ccctttcgga aataaaaatt attccggaag gggtttttgg
      181 gttgttctag atcaaaccaa cttttttcat ggctgcggcc aaacggtcga tgactgcttg
      241 atcggcatcc gtcaaaggca gacgcatcgg tccgacattg aagcccaaca attgcattcc
      301 tgctttgact ggaatcggat tcggctccac gaacaggcag tcgatcagtt ctttgtactc
      361 ggcctgcatt gctgcagctt ttttgatgtc gccatctagc caagcgtggg tcatttcggc
      421 tgtagctttc ggtgcgatat tggcaaggac ggagatgacg ccttttgcgc ccaagctcat
      481 cagcgggatg atcgtgtcat cgttacccga gtagatgtcc aaacgatcgc ccaaaagacg
      541 caggttgtcc actgcctggc cgaagttgcc ggtagcatcc ttcaacgaaa cgatgttgtc
      601 gattttcgcc aactccacca aagattctgg ggtaatattc tgaccggtac ggccaggcac
      661 attgtaaagg acgatcggca ctttcacgct gttggcgata gctttgtaat gttcaatcaa
      721 gcctttttgc gatgttttgt tgtagtaagg ggtgacgctg aggattgcat ccacacccgt
      781 tgcttcgaca gcgcgcgtca atccgacgcc gtggcgcgta tcgttgcttc cggcaccgcc
      841 gatgacgggt acgcggccat tgactttttc gacagtgaag cggatcagat ccagctgttc
      901 ctcatccgtc agcgtggaag cctctccggt tgttcctgcg ataatgatcg agtcggttcc
      961 gttcgcaatc tggaactcga tcagttcttc caatacctcg tagttgattt cttggttctc
     1021 cgtatcttta aacggcgtaa caatagcgac gcctgagcct tcaaaaatag ccatgcttat
     1081 cttcctcctg aattctgtgt tttatttatg aatagacaaa aaacgactat gagcaaatgg
     1141 tctcatagtc gttcaaaaca ggctgcttaa gctgaaacat tgcatccaga tagtgaattc
     1201 caccaatttg ccccaacgtg agaagctcac cgaacaaagc tgggcggctt tgtccgacag
     1261 tgccagattt attcaacctt ggccccaata cagagtattt cggcagcaac acctttcagg
     1321 gaacccctta ctgattgtta accgcgacct cttcatcaca tcgaatcgta ttttgttaac
     1381 tccacactat caaatacaaa tcagtctgtc aatcatattc tgagtggatg ctgagaaaat
     1441 gctttgaaaa aaatggcaaa aaagacacat tcggtgacag tgcatccgga tgctccggga
     1501 aagcatatgc gccagcaatg tgataaaatc aggttgaggg aacgctttcg agggaagtcc
     1561 agaaagggat tccgggatgt tttgttgcag gccatccgcg cagagtttgt ctccagcgca
     1621 ccggcaagga tttgagagga cgtgaaaact tatgaagata gaacatgttg cagtctgggt
     1681 ggatgatttg gagctgatgc gcgattttta tgttcgtttt tttgacggaa aggctaataa
     1741 attatatcgg aatcctgaaa aagacttcca atcttacttc attaccttcc gtgaaggagc
     1801 ccgtttggaa cttatgaaga ggatgaatgt cacggaacgc tatccttttg atatgctggg
     1861 ctgggcgcat ctggctgtct ccgtcggcag caagcagaac gtcgactttt tcaccgacag
     1921 gctcatcaaa gcggggtaca catgcaaaag ccaaccgcgc gtgacggggg atggctacta
     1981 cgaaagcatg ttccttgatc cagaaaagaa tgtaatcgaa atcaccatct gaaagaacta
     2041 tgaggccgaa aatcaggggg gctgccatga tgaaaagaaa ccatctattt gccgatcaaa
     2101 cgaaagcagg aagggatatg cgcaaaaaga ggacgcgctt tctgattgct ggagcgatcg
     2161 gtaccagtat cggcttgaca gccggtttcc tgttacgttg ggaattgacc cctttgatcg
     2221 gttgggacag tgccgcaatc attttattgt atttgctgtg gaaggatttt catcctcatg
     2281 atgggacgga aacggaacgg attgcgcaag aagaggatat ccaatattcg acgatcgatt
     2341 tttttgtcct gttggcatgt ttgatcagta tcggggctgt cgcgttgatg ttgaccaccc
     2401 aagaacgcag tttcgagaca atcggatttg gcctgttcag catcgtactc tcctggacga
     2461 cggtgcatgg actctatatg ttacggtatg cggaactgta ttatcgcggt gtgaacggcg
     2521 gcgtggtttt tgaagggacc gagaaaccta atttttggga tttcgcttat ctggcgttca
     2581 caatcgggat gacttatcag gtctccgaca cgacttttac gacaccggaa tttcgtaagg
     2641 ctgcgctggg gcacgccctg ttgtccttca ttttcgggac agccatcatt gccacgacca
     2701 tcaattttgt cgctagtttg agcagatgaa ggaggagttt atgcatgttt tttaaatcat
     2761 tttttgatcc gcacatagcg caattttcct atttagtggg gtgtcaaaaa acgggagaag
     2821 ccattattat cgaccccctg cgggccttgg atgattatat ccaggcagcc gaggatgaag
     2881 gattggtcat cactgcagcc acggaaacgc atatccatgc cgattatgca tccggtcttc
     2941 aggaaagcgg aaggcgctta ggtgccaaac tgtatgtatc ggacttgggc ggggatgact
     3001 ggcgctatca gaatttgcct gaaggtagtg tgctgctgca ggatggggac atcatttctg
     3061 tagggaaagt caaactggaa gtgttgcaca cgccgggaca tacgccagag agcgtgtcat
     3121 ttgtattgac ggacatcggc ggcggttcgg acattccaat gggagtgttc accggcgact
     3181 tcatctttgt gggcgacgtt ggacggccgg acttgctgga agaagctgcg catatgcaag
     3241 ggaccactga aatcggagca aaagcgatgt tccgttcctt gcagaaaatg gctgactatc
     3301 cggaccatct gcagatttgg ccgggacatg gcgccggcag cgcctgcgga aagtcgctgg
     3361 gagccgtttc gatgaccact ttgggctacg aaaaatacaa caactgggct ttccaatatg
     3421 atgaagaaac gagctttatc gaggcgttga cgcaggacca gcctgagccg cccagctact
     3481 ttgcacaaat gaagaaaatc aacaaacggg acagcggcgc ctatgtaccg tatccgattt
     3541 tcccgctgca acaggctggg ccgaatgata ggatgatcga tctgcgcgcg aaagagatat
     3601 atcaagccgg ccatatcgaa agaacgctga atattccgtt gaacaaaaag tttttgactt
     3661 atgtggggtg gttcctggat tacgacgagc aagtgacgct catcggaacc aaagaagatg
     3721 ctgaaacagc tacccgtcaa ctgcagctga tcgggttcga ccaagtcaga ggctatctgg
     3781 atgccgggca aatcgctggc ggtaagatga ctgaaacgat caccgcagca gccttcatcg
     3841 ctttgcgcca agaaaaggat ctgcagatat tggatgtgcg ctcgcagagc gaatggaatg
     3901 aagggcatct ttccgacgcc aaacgcgtgc tcctcggaaa acttttggag gccccgttgc
     3961 cgtttaaaag ggatgaacct ctttacgtcc attgtcaatc cggtgtccgt tcggcagttg
     4021 ccatcggcgc cttggaggag cggggattca aaaaaatcat caatatcctg ggaggataca
     4081 ctgcgatcga aaacagcctc aatggataga cacaaatgcg cgaggacctg caaaaaggta
     4141 atcagcacaa caaaatgagc cggaaggaag agctgcaaaa ggcccttcct tccggctgct
     4201 ttttgattta aatcgtttct atcaagacgc ggggaataaa tagccagaat ccctgtacga
     4261 tggtatacta ctaattgtcg catttagtga agtagaccga atagtccatg agttttg
//
LOCUS       DAOMUJ010000110         4291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5804_length_4291_cov_3.370633, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000110 DAOMUJ010000000
VERSION     DAOMUJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5804_length_4291_cov_3.370633"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1276)
                     /gene="ugpC"
                     /locus_tag="PLT73_03950"
     CDS             complement(167..1276)
                     /gene="ugpC"
                     /locus_tag="PLT73_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRM19568.1"
                     /translation="MVEIQLNSIHKKYDNAANYSVTDFNLHIQDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELIIGDTVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVDNAADILGLTEYLDRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHRRLNTTTIYVTHDQTEAMTMADRIVIMKDGFVQQIG
                     SPKEVYDTPVNVFVAGFIGSPAMNFFNVTLQDGVITNGEGLKLTLPAGKRRVLEERGY
                     NGKELIFGIRPEDIQSEQIVIDTNPTWAVKAEVVVSELLGAETMLYSKVGGTEFISRV
                     DARDFHSPGEVIDLAFNMNKSHFFDMQSQDVIAIP"
     gene            1557..2102
                     /locus_tag="PLT73_03955"
     CDS             1557..2102
                     /locus_tag="PLT73_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRM19569.1"
                     /translation="MSKRGFEVISSYADKNIQLPKRATKHAAGYDFEAAEEIVLPAIW
                     KTIVKSAGLQLGELFGSDNDEAIEKKEELLRPLLVPTGIKAYMQEDEYLQLTNRSSNP
                     LKNFIVLPNGVGIVDADYYNNEGNEGHIYFQFLNFGLRDKVIHKGDRIGQGIFLQFLK
                     ADQDEAEGSERTGGFGSSGQA"
     gene            2206..3588
                     /gene="radA"
                     /locus_tag="PLT73_03960"
     CDS             2206..3588
                     /gene="radA"
                     /locus_tag="PLT73_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRM19570.1"
                     /translation="MAKKNAVKYVCQACGYESPKWLGRCPNCNTWNQMEEEKEATKAV
                     KQQQRSNFSGNVPEAIAIQDIKTENLPRVKTQMEEVNHVLGGGIVPGSLILIGGDPGI
                     GKSTLLLQVSAQINQGGHRVLYISGEESASQIKLRAERLGVKGTDFYIYPETDIDAIR
                     NTIERIKPEFVIVDSIQTMIHPDNTSTAGSVSQVRECTAEFMRIAKSNDIAIFIVGHV
                     TKEGAIAGPRMLEHMVDTVLYFEGDRHHAFRILRAVKNRFGSTDEIGVFEMIEGGLRE
                     VRNPSELFLEERLAGGSGSAVVASLEGTRPILAEIQCLITPTVFGNARRTASGLDYNR
                     VSLIMAVLEKRAGLLLQNQDAYFKSTGGVKLDEPAIDLAVAVSIASSYWDSETSATEC
                     FIGEIGLTGEIRRVSRIEQRVNEAEKLGFTKVYLPKNNMAGWKHPKGIQIIPVATLQE
                     TLRKVFPNKN"
     gene            3615..>4291
                     /locus_tag="PLT73_03965"
     CDS             3615..>4291
                     /locus_tag="PLT73_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRM19571.1"
                     /translation="MKRKIITAVFLMVGGSAGITALPYLWELAGIRNNLFNNVFVNFG
                     VGALIFYLLSFLLMKLILDIIQKIENYFSTLSVSYMLFGSIGTIIGLVLAWLIGISLT
                     SLRIPVINDVLPAILSLILAYFGFRVGTTRIEEFRKLFAPKPKKQEETQMLDRKADDT
                     FRKYKILDTSVIIDGRIYDIAKTGFLEGVIVIPNFVLRELQYIADSSDSLKRVRGRRG
                     LDILNKL"
BASE COUNT         1102 a         1010 c         1139 g         1040 t
ORIGIN      
        1 tggaaggtcc ttcgccggag ctgcgcttgc attccgccgc cctcctccgg ccaactaaac
       61 cccataagaa aaaggctgtg ctcaatgaac gacagccttt ttctatgggg ggggagggta
      121 ggttagaact ggtaagcgta attagggtga tgcctccatc ctctggttaa gggatggcga
      181 tcacatcttg ggattgcatg tcgaagaagt gggacttgtt catgttgaag gccaaatcaa
      241 tgacctcgcc tggcgaatgg aaatcgcgcg cgtccacacg ggaaatgaat tctgttccgc
      301 cgactttact gtaaagcatc gtttcagcac ccaacaattc ggatacgact acttccgctt
      361 tgacggccca agtcgggttg gtatcaataa cgatctgctc gctctgaatg tcttccgggc
      421 ggatgccgaa aatcaattct ttgccgttgt atccacgttc ttccagaact ctgcgttttc
      481 cggccggcaa ggtcaattta agcccttcgc cgttcgtgat gacaccatcc tgcaaagtaa
      541 cgttgaagaa gttcatggca ggcgatccga tgaatccagc tacgaaaacg ttaaccggtg
      601 tatcgtaaac ttctttcggg ctgccgatct gttgcacaaa tccatccttc atgatgacga
      661 tgcggtctgc catggtcatc gcttcggttt ggtcatgggt tacgtagatg gttgtcgtat
      721 tcaggcgacg gtgcaatttt gcgatttcag cacgcatggc cacacgcaat tttgcatcca
      781 agtttgataa cggctcatcc atcaggaaga cttttgcatc acgcacgatt gcgcggccca
      841 aagcgacacg ctgtctttgc ccaccggaca aagctgccgg tttgcggtcc aagtattccg
      901 tcaagcccaa gatgtctgct gcgttatcga cgcgcttctt gatttcatct ttgctgtact
      961 ttctcaattt cagaccgaat gccatattgt cgaatacagt catatgcgga tatagggcat
     1021 agttctggaa taccatcgcg atatcgcggt cttttggtgc cacatcgttc atgacggtat
     1081 cgccgatgat caattcacct tcggaaatat cttccaaacc ggcaatcata cgcagtgttg
     1141 ttgactttcc gcaaccggaa ggaccaacaa agacgatgaa ttcgcgatct tggatatgca
     1201 agttgaaatc tgtaaccgaa taattagcgg cgttgtcgta ttttttgtgg atactgttca
     1261 attggatctc taccatttat ttgtacctgc ctttttggtt tttttggctt ttctttatgt
     1321 ccttattcta tttgaaagcg gattccgcga ccacgggaaa ctgaccaaaa aaagcacctg
     1381 ttttttgtgc gttttgccga aggcgctttc caatcgcaaa aaatgttcag aaaatcctta
     1441 ggaattgaca ggtgcacaac taaaccgtga aaaacaaccg ttgttttggt aaaatggttc
     1501 tttgatgcat gaaaaatcgc tgaagcgata ctagagagaa aaaggggttt tgatccatga
     1561 gcaaaagagg ttttgaagtc atcagcagtt atgccgataa aaacatccaa ttacccaagc
     1621 gtgcaacgaa acacgcagcg gggtatgatt ttgaagcagc cgaagaaatc gtgctgccgg
     1681 cgatctggaa aacgatcgta aaaagtgcgg ggttgcagct tggagaactg ttcgggagcg
     1741 acaacgatga ggcaatcgaa aagaaagagg agcttttgcg tccgcttttg gtgcctaccg
     1801 gaatcaaagc ctacatgcag gaagatgaat accttcaatt gacgaaccgc tccagcaatc
     1861 cactgaaaaa tttcattgtg ctgccgaacg gtgttggcat agtcgatgcg gattattaca
     1921 acaacgaggg caatgaaggg cacatctact tccagtttct taattttggc ttgcgcgaca
     1981 aagtcatcca taagggtgat cgtatcggcc agggcatctt cctccagttc ctgaaagcgg
     2041 accaggatga agcggaagga tcagaacgca caggcggttt cggctcgtca ggacaggctt
     2101 aaaaagaaaa gccaaatcag tgggtatttg cgtattgtta tacgtagagt aggctttttg
     2161 tgataaaata agataagttg attcatactt gaaggagaga ttcatttggc aaagaaaaat
     2221 gcagtgaaat acgtttgtca ggcttgcgga tacgagtcgc cgaaatggct gggacgttgc
     2281 ccgaactgca acacttggaa ccaaatggag gaagaaaagg aagcgacgaa agccgtgaag
     2341 caacagcaac gttccaattt cagcgggaac gtacctgaag cgatcgccat ccaggacatc
     2401 aagaccgaaa acctgccgcg tgtcaaaacg cagatggaag aagtcaacca tgtgctgggc
     2461 ggcggcatcg tgcccggttc gttgattctg atcgggggcg acccgggaat cggcaagtcc
     2521 accttgctgc tgcaggtttc tgcgcagatc aatcaaggcg gtcaccgcgt tctgtacatc
     2581 tccggagagg aaagcgcgag tcagatcaag ttgcgggcgg aacggctagg ggtcaagggc
     2641 accgacttct acatctaccc ggaaacggat atcgacgcga tccgaaacac gatcgagcgg
     2701 atcaagccgg aattcgtcat cgttgactcg atccagacga tgatccatcc ggacaacacg
     2761 agcacggccg gcagcgtgtc gcaagtgcgc gaatgcacgg ctgaattcat gcggatcgcg
     2821 aagagcaacg acatcgcgat cttcatcgtc gggcatgtga cgaaggaagg cgcgattgcc
     2881 ggtccacgga tgctggagca catggtggat acggtgctgt attttgaagg cgatcggcat
     2941 catgctttcc ggatcttgcg cgccgtcaaa aatcgcttcg gatcgaccga cgaaatcggc
     3001 gttttcgaaa tgatcgaggg cggtctgcgt gaggtgcgga atccttccga gctgttcctg
     3061 gaagaacggc tcgccggggg atccggttcg gcagtcgttg cctcactcga gggcacgcgt
     3121 ccgattttgg ccgaaatcca gtgtctgatt acgccgacag tcttcgggaa tgcccgccgg
     3181 actgcaagcg gcttggacta caaccgggtt tcgctgatca tggccgtgct ggaaaagcgg
     3241 gcgggcttgc tgctgcagaa ccaggatgcc tacttcaaat cgacaggtgg cgtgaagctg
     3301 gatgagccgg ccatcgacct ggcagtagcc gtcagcatcg cctccagtta ttgggatagt
     3361 gagacttccg cgaccgagtg ttttatcggt gaaatcggcc tgaccgggga aatccgacgg
     3421 gtgagccgga tcgagcaacg cgtcaacgaa gctgaaaaat tgggcttcac gaaggtgtat
     3481 ctgccgaaga acaatatggc tggctggaag cacccgaaag ggatccagat cattcctgtc
     3541 gcaacgcttc aagaaacgct gcgcaaagta tttccgaata aaaattgatg acagaaggaa
     3601 agagagggaa gcctttgaaa cggaaaatca ttacggccgt cttcctgatg gtgggcggca
     3661 gcgctggtat cacagcactg ccttacctgt gggaattagc gggcatacga aacaatcttt
     3721 tcaataacgt ttttgttaat tttggggttg gagcacttat attttattta ttgtcattct
     3781 tattgatgaa attgattttg gacatcatcc aaaagataga aaattatttc agtaccttat
     3841 cggtcagcta catgttgttc ggttcgatcg gaacgatcat cgggcttgta ttggcttggt
     3901 tgatcggaat ctcgttgaca tcactgagga taccggtcat caacgatgtg ctgccggcta
     3961 ttttgtcctt gatcttggct tacttcggct tccgcgtggg gactacccgg attgaagaat
     4021 tccgtaaact gttcgcaccg aaaccgaaaa agcaggaaga aacacagatg cttgaccgca
     4081 aagcggatga cacattccga aaatacaaga ttttggatac gagcgtcatc atcgacggcc
     4141 gcatctacga catcgccaaa accggctttc tggaaggcgt catcgtcatc ccgaacttcg
     4201 tattgcgcga actgcagtat atcgcggatt cctccgacag cctgaagcgc gtcaggggca
     4261 gacggggatt ggacatcctg aacaaactcc a
//
LOCUS       DAOMUJ010000111         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5814_length_4285_cov_1.953664, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000111 DAOMUJ010000000
VERSION     DAOMUJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5814_length_4285_cov_1.953664"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /gene="pheT"
                     /locus_tag="PLT73_03970"
     CDS             <1..1410
                     /gene="pheT"
                     /locus_tag="PLT73_03970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM19572.1"
                     /translation="ETVTVALEAALFNPVLIRKTAGKFNLRSESSSRFEKGINVATIR
                     TAGQHAAELIHELAGGTIVAGTASVDTVEVKDTEVVITLEKINRSLGTAISSGEVTAI
                     FNQLGFASTFDGEIFTVAVPPRRWDISIYADILEEVARIYGYDNLPETLPITPALPTA
                     LTPKQHTMRITRRFMEGAGLTQNISYVLTTAEKAREYAVEDKEGIRLAWPMSEDRSTL
                     RMNLLSTLLDNAAYNVARKNTDIQFYEIGRVFFPSADSVLPIEAERLAGVMTGMAYQK
                     DWQMAAEPVNFYHAKGVLDGYFETMGLSDQIRFEAAKDLKWMHPGRTAAVYLGDAYIG
                     YVGQVHPATANAYDLKETYAFEIDFEAIIAAPKEVITQQPIPKFPGVTRDVALLVDET
                     VTHQQIVKTIKENGGKFLKDVHLFDIYQGKGIEDGKKSVAYSMSFLNPEATLVDEDIN
                     KAFTKLVAALETECGAAIR"
     gene            complement(1590..>2226)
                     /gene="rnhC"
                     /locus_tag="PLT73_03975"
     CDS             complement(1590..>2226)
                     /gene="rnhC"
                     /locus_tag="PLT73_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRM19573.1"
                     /translation="DEVGNGSYFGPLTVCAVYASADQLPLLKKMGVQDSKALTDARII
                     TIAKQLLEHVPHSVINLMPEKYNEVQPTMSQGKMKALLHNQVLDQLLKKIEPEKPKAI
                     LVDQFELPSTYFKHIADQKVQVKENIYFQTKGESHHLAVAAASIIARYYFLKTLADMT
                     AASGYPIPSGAGANVDLIAAEMYREGGLELLDKYTKLHFANTQKAIRLANR"
     assembly_gap    2227..2236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2237..2519)
                     /locus_tag="PLT73_03980"
     CDS             complement(<2237..2519)
                     /locus_tag="PLT73_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3378 domain-containing protein"
                     /protein_id="HRM19574.1"
                     /translation="MSNVVLKVTMGQLKKLAVHYQDYAVKPVPHSLFTAKKNQTTITG
                     YNSGKVLFQGQNAESEAAQWQASGDQVVATKSHSAKNGLKKEIPNKTGLP"
     gene            2741..>3290
                     /gene="zapA"
                     /locus_tag="PLT73_03985"
     CDS             2741..>3290
                     /gene="zapA"
                     /locus_tag="PLT73_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRM19575.1"
                     /translation="MTENKRRFKTIIAGKTYTIVGNKAPEHLTAVSELVNAQLEQIKQ
                     AAPALGREERGILVAVNAISDQITKQLEMEELLLKVAKLEKALQESQSVTHSASVEET
                     EQTEPENTLAETPKPETIADVEQKTEAVPLAEEKVAIQTSESKTAVVETPEKEHNAEK
                     MADSAAAKKALPEFSRKKRDDKS"
     assembly_gap    3291..3304
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3305..3527
                     /locus_tag="PLT73_03990"
     CDS             <3305..3527
                     /locus_tag="PLT73_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19576.1"
                     /translation="ATISELQQAAREANELPARKSNSKLTKTTTASEATLKRVQHRNQ
                     VRSALGVNRNAENTIPPYTKNHEHDGKKK"
     gene            3554..4099
                     /locus_tag="PLT73_03995"
     CDS             3554..4099
                     /locus_tag="PLT73_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRM19577.1"
                     /translation="MLTFIIVLTLLWGIYTGVRRGLILQIVYTVGYFISFLVAREYYT
                     VIAAKIDLLVPYPSIEFGKELIFYTEEVSFVLDQAFYNGLAFILLLFAGWLVTRFVGS
                     MLNSLAFFPIIKQLNQLGGGVLGFLMHYIGIFLLLTLASMIPMDFIQQLYVESDLAAW
                     MVSETPYFSRLIYDWWIGIIQ"
BASE COUNT         1146 a          967 c         1085 g         1063 t
ORIGIN      
        1 gaaaccgtga ctgtcgcttt ggaagcggct ttgttcaatc cggttttgat ccgcaaaaca
       61 gctgggaagt tcaacttgcg cagcgaatcc agttcccgtt tcgaaaaagg catcaacgta
      121 gcgacaatcc gcacagccgg acaacatgcc gctgaactga tccatgaatt ggcagggggc
      181 actatcgttg ccggcacggc ttcggtcgat actgtggaag tgaaggatac ggaagtagtc
      241 atcactttgg aaaaaatcaa ccgttcgttg gggaccgcca tttccagcgg tgaagtgact
      301 gccatcttta accagttggg ttttgcctcg acgtttgacg gcgaaatatt caccgttgct
      361 gttccaccga gaagatggga tatctccatc tatgcggata tcctggaaga agtcgcacgc
      421 atctacggat acgacaacct tccggaaaca ttgccgatca caccggcttt gccgactgca
      481 ttgacgccta aacaacacac gatgcgcatc accagaagat tcatggaagg tgcgggactt
      541 actcagaaca tcagctatgt cttgactacc gccgaaaaag cgcgggaata cgcagtcgaa
      601 gacaaggaag gcatccgttt ggcttggccg atgagcgaag atcgcagcac gctgcggatg
      661 aacttgttga gcacgctttt ggacaacgcc gcttacaacg ttgcccgcaa gaacacggat
      721 atccagtttt acgaaatcgg tcgcgtcttc ttcccgtctg ctgacagcgt tttgccgatc
      781 gaagcggaac gtttggccgg cgtgatgaca ggaatggcct accaaaaaga ttggcaaatg
      841 gctgccgaac ccgtcaattt ctaccacgcc aaaggggtat tggatgggta tttcgagacg
      901 atgggcctga gcgatcagat ccgttttgaa gctgccaaag acctgaaatg gatgcatcca
      961 ggcagaacgg ctgccgtcta tctgggcgat gcctatatcg gttatgtcgg ccaagtccat
     1021 ccggcaacgg caaatgccta tgacctgaag gaaacgtatg ctttcgaaat cgatttcgaa
     1081 gcgatcatcg ccgcaccgaa ggaagtgatc acgcaacagc cgatccctaa attccctggc
     1141 gtgacccgcg atgtggcttt attggtggat gaaaccgtaa cacaccaaca gatcgtcaag
     1201 accatcaagg aaaacggcgg gaaattcctg aaggatgtcc acctgttcga tatctatcaa
     1261 ggcaaaggca tcgaagacgg caagaagtcg gttgcttatt ccatgtcatt cctgaatcct
     1321 gaagcgacgc ttgtcgatga agacatcaac aaagccttca cgaaattagt cgcagctttg
     1381 gaaaccgaat gtggagctgc catccgttaa tttagaagaa tgatgtacaa acaaagagtg
     1441 gaccgtctcg ggatgagacg gtccactctt tgttttgcga agattaagat gtggctagta
     1501 atcagttgtc cgattcattc ggattgtcgg acaacctact cagcatcgga cccagctgaa
     1561 gggaattctc cctacgattc gccggtaagc tatctgttcg ccaatctaat ggctttttgg
     1621 gtgttggcga agtgtagttt cgtgtatttg tccagcagtt ccaagccgcc ttcacgatac
     1681 atttccgcag cgatcagatc gacgttggcg ccggcgcctg aagggatcgg ataaccggag
     1741 gctgcggtca tgtccgccaa agtcttcagg aagtagtagc gggcgatgat ggaagccgcc
     1801 gccaccgcca gatggtggga ttcgcctttt gtctggaaat aaatattttc cttcacttgc
     1861 actttttgat cggcgatatg tttgaaatac gtggaaggca attcaaactg atcgaccagg
     1921 atcgctttgg gcttttccgg ctcgattttc ttcaacagct gatcgaggac ttggttatgc
     1981 agcaatgcct tcattttacc ttgagacatc gttggttgga cctcattgta tttttcgggc
     2041 atcaagttga tgacgctgtg cggaacatgc tccaacaatt gcttcgcaat ggtgatgatg
     2101 cgggcatcgg tcaaagcttt tgagtcttga acgcccatct ttttcagcaa cggtaattgg
     2161 tcggcggatg catagacggc acagaccgta agtggtccga aatagctgcc gttgcccact
     2221 tcatcgnnnn nnnnnncagg gagcccggtt ttgttaggaa tctctttttt cagtccgttt
     2281 tttgcggaat gggatttagt ggccacaacc tggtcgccgg atgcttgcca ctgcgctgct
     2341 tcgctttcgg cgttctggcc ttgaaacagc acttttccgg agttgtaacc agtgatggtg
     2401 gtctgattct ttttggcggt gaaaaggctg tgcggcactg gcttcacagc ataatcctga
     2461 taatggacgg ccaatttttt tagttgaccc atcgttactt tcaatacgac attcgacata
     2521 ctctcatcct tgctctattt aatttctctg tactgcttat ttattatagt attccttatt
     2581 ctatcacatg aagttgtctt cttcatgggg aattgctgca gagtggatca gctattttag
     2641 gacagcagga caggattggc aacataaaga ggtttatact gcaattcgtc ggatttatgt
     2701 taaaatatat tagacagttt tttaatggag gcatttgaat atgacggaaa acaagagaag
     2761 attcaaaacg atcatagcag gaaagaccta tacgattgtg ggaaataaag cacctgagca
     2821 tttgactgct gtatcggagt tggtcaatgc gcagttggaa cagatcaaac aagcagcacc
     2881 ggcgcttggc cgggaggagc gcggcatcct agtggctgtg aacgccattt ccgatcagat
     2941 cacgaaacaa cttgagatgg aagagctgtt gctgaaggtg gcgaagctag agaaggcttt
     3001 gcaggaaagc caaagcgtta cacactccgc ttccgttgag gaaacagaac agactgagcc
     3061 ggagaatact ttagccgaga cacctaaacc tgaaacgatt gccgacgttg agcaaaaaac
     3121 tgaggctgtt cccctggcag aggagaaagt agctatacaa acaagtgaat ccaagacagc
     3181 ggtggttgaa acgcctgaaa aggaacacaa tgcagaaaag atggccgata gtgcggccgc
     3241 taagaaggcc ttgcctgaat tttcccggaa aaagcgcgac gacaagagtc nnnnnnnnnn
     3301 nnnnagcgac aatcagtgaa ctgcagcaag cggcccgcga agcaaatgag ttgcctgcca
     3361 ggaaatccaa ttcgaaattg acgaagacga caaccgcttc cgaggcgacg ttgaaacggg
     3421 tacaacacag aaatcaagta cgttcggctt tgggtgtcaa cagaaatgca gaaaatacca
     3481 tcccgccata cacaaagaat cacgagcacg acggaaaaaa gaagtaaaaa gactgtagaa
     3541 atcaggtgaa aaaatgctta cgtttatcat agtgcttacg ttgctgtggg gcatctatac
     3601 gggcgtcaga agaggtctta ttctgcagat tgtctataca gtggggtatt tcatatcttt
     3661 cttggttgcc agagaatact ataccgtgat cgcagcaaaa atcgatctgc tggtcccata
     3721 tccatccatc gagttcggta aagaattgat tttttacaca gaagaggtat cgttcgtcct
     3781 ggatcaggcg ttttataacg gattggcgtt catcttgttg ttgttcgcag gctggctggt
     3841 cacgagattt gtcgggagca tgctgaactc attggcattc ttcccgatca tcaaacaatt
     3901 gaaccaactg ggaggcggcg tccttggatt cctgatgcac tacatcggaa tcttcctgtt
     3961 gctgacgctc gcatccatga ttccgatgga tttcattcaa cagctctatg tcgaaagcga
     4021 tcttgcggct tggatggtgt ccgaaacgcc ttacttttct cgcttgatct acgactggtg
     4081 gatcggcatc attcagtaaa gacagttggt taaacagaaa gggaccgtat agcgttacta
     4141 tatcgtcccg cttttttgga gaaagcactt ttaggagaat cagatgaata ataaaataag
     4201 caaaacgtta gagttcgaaa aaataaaaca acaactccag aattttgcgg caacagatca
     4261 agggatagat aaaattcagg cattg
//
LOCUS       DAOMUJ010000112         4258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5864_length_4258_cov_2.159648, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000112 DAOMUJ010000000
VERSION     DAOMUJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5864_length_4258_cov_2.159648"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLT73_04000"
     CDS             <1..772
                     /locus_tag="PLT73_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRM19578.1"
                     /translation="DIFLFNKDLEEDIRFMKEGIENGILKLERIDEAVTRILAMKAAL
                     KLPEKQADALLVPAKEELAVLQAEQHVQWATEAADNGITLVKDTAGNLPLRPDATPRL
                     LLQLLGPYEESNTQIFSKVLPLLEKEGFQVTLYQPEDFTTGFESVSQFRSKYDAVLYL
                     ANVENRSNATTTRINWYTFFGLGNNIPWFVDEVPTVFASLGNPYHLMDVPMVKTYINT
                     YDNHQAVIEALVAKILGKSDFKGVSPIDPYCGLMDARR"
     gene            856..>1639
                     /locus_tag="PLT73_04005"
     CDS             856..>1639
                     /locus_tag="PLT73_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRM19579.1"
                     /translation="MFELDRILKEETKVEKEQRKSGFNVTDPDILDELILSGNKFRLN
                     GNFLLSEEMFFKKGDVHIRKHNRYSDMPLHHHQFLELNYMYSGSCTQEVSGEKIVLNE
                     GDIIMLDRGSSHSFQALGENDILINVLLKVETINTKILQQLVRKRGVLTEFLVLASQR
                     IHEEDQFLVFRTGENAKVQTFMQYILEEYYGGTEPNTEVIHLYLPLLFMELARTFEEE
                     TKGQQGHQNQVVTEALAIIEKDYRTLSLEMLAQQLGFNKNYLG"
     assembly_gap    1640..1672
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1673..1853
                     /locus_tag="PLT73_04010"
     CDS             <1673..1853
                     /locus_tag="PLT73_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM19580.1"
                     /translation="LQRQRMLHAHHLLVFSNYSMEEISEEIGYLDTSYFFRSFKKTHG
                     KTPGQIREEALHRDL"
     gene            1950..3326
                     /locus_tag="PLT73_04015"
     CDS             1950..3326
                     /locus_tag="PLT73_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM19581.1"
                     /translation="MATETRILDQTDENKLPLSEKLTFGFGNLAANLMLTTASSFITY
                     YYTDVVGLSAVIVGNILLWARLFDGASDLAMGAIVDKTRSKHGKARPWILWMAIPYAL
                     AMILLFTSPDFLGSTGKAVYAFVTYVIALAGVYTATMVPYNAMIGTTTSNPVDRGNLS
                     TSRTLAGFVGAMGVTAAVLPIVNFFGGDKQAWTWMAALFGGISTVLLLILFKNSKERV
                     IESAVAVEKVPLKKNLKALLQNKYWVIMILYMLISFISSGLGGINIFYAQWILGDPAK
                     VAVIGILSFMPIAVGAVFMPVLLSKFSKKTITLIGGIVMMIGLLIIAVFPENFTMIMV
                     GLIIRGLGIAPGAVAGFAMLGDVADYGEWKTGIRSEGLIFSAGTFAEKVGSGVGGLIL
                     GVVLGLGGYVSQGATQSTEALFAIKAIFAYLPLAFTAICILLILFYDLDKKLPEIAED
                     LKAKRVNG"
     gene            3530..>4258
                     /locus_tag="PLT73_04020"
     CDS             3530..>4258
                     /locus_tag="PLT73_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HRM19582.1"
                     /translation="MNIYNLKTNYLMNPIGFLMDTVALSWIIEAPFEEELQGIRVLIA
                     LDENFEEVIHDSRMQADLDSLCYQPEIKLQPRTRYFWKVIAKNVSGDESSSEIHYFET
                     AKEEELWTGKWIEPARQLQRMPLARKLFRLPAKPIRKARVYATGLGIYELYLNGEKIG
                     DEYLAPGFHSYDFWQQYQTYDVTEALQAGGNAIGFMLGDGWFKGRFGFDGGYEDLYGD
                     QFRLIAECIVTYEDGTEEKITTDET"
BASE COUNT         1127 a          949 c         1091 g         1058 t
ORIGIN      
        1 tgatatcttc ctgttcaaca aagacttgga ggaggatatc cgcttcatga aggaagggat
       61 tgaaaatggc atcctgaagt tggaacgcat cgatgaagcc gtcacccgca ttctggcgat
      121 gaaagcagca ttgaagttgc ctgaaaaaca ggcggatgca ttgttggttc cggcaaagga
      181 agaactggcc gtcctgcagg cggaacaaca cgtccaatgg gctaccgaag cagccgataa
      241 tggcataacg ctcgtcaaag atacagccgg taacctaccg ctacggccgg acgcgacgcc
      301 tcgtctgtta ctgcaactgc tcgggcctta cgaggaatcg aatacccaaa tcttttccaa
      361 ggtgctgccg ctgttggaga aggaaggctt ccaggtgacg ttgtatcagc cggaagattt
      421 cacgaccgga ttcgaatccg tttcccagtt ccgttccaaa tatgatgccg tcctctatct
      481 ggccaatgtc gaaaaccgca gcaacgccac aacgacaaga atcaactggt acaccttctt
      541 cggtctaggg aacaatatcc cgtggttcgt cgatgaagtg ccgaccgtat ttgcgagtct
      601 ggggaatccg taccatctca tggatgttcc aatggtgaag acctacatca atacctacga
      661 caatcaccaa gcagtcatcg aagcgcttgt agctaaaatt cttggcaaga gcgatttcaa
      721 gggcgtgagt ccgattgatc cttattgtgg gttgatggat gcacgcagat agattttatg
      781 cacccaaccc gatgagtccg gttgggtttt tctgtataat ggtggaggag acaatgagag
      841 agggtgagcc gaaaaatgtt tgaactggac cgtattttaa aggaagaaac caaagttgaa
      901 aaagagcaac ggaaatccgg gtttaatgtg acggatccgg acattctcga tgaattaatt
      961 ttgagtggga ataagttccg cttgaatggc aatttcctac tgagtgagga aatgttcttc
     1021 aaaaaagggg acgtccatat ccgtaagcac aatcgttatt cggatatgcc cttgcaccac
     1081 catcagtttt tggaattgaa ctatatgtat tccggttcct gcactcaaga agtgagcggt
     1141 gaaaaaatcg tcctcaatga aggtgacatc atcatgctgg atcggggcag ctcgcattcc
     1201 ttccaggcac tgggcgaaaa cgacatcctc atcaatgttc tgttgaaagt ggagacgatc
     1261 aataccaaaa tacttcagca actcgtgcgc aaacgcgggg tattgactga atttctggta
     1321 ttggcaagcc aacgcattca cgaagaagac cagtttttgg tattccggac gggagaaaat
     1381 gcaaaagtcc agactttcat gcagtacatc ctggaggaat actacggcgg aactgaaccg
     1441 aataccgaag tgatccacct ctacctgcca ttgttgttca tggaactggc gcgcacgttc
     1501 gaagaggaaa cgaaagggca acaaggccac cagaatcagg ttgtcacgga agctttggcc
     1561 atcatcgaaa aagactatcg cacgctttct ttggaaatgt tggcgcagca attgggtttc
     1621 aacaagaatt atttagggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctgcagc
     1681 gccaacgcat gctgcacgcg catcatctcc tggtcttttc aaattattcg atggaggaaa
     1741 tatccgagga aatcggctac ctggacacga gttatttctt ccggtccttc aaaaaaacgc
     1801 acggcaagac gccagggcag atccgcgagg aagctttgca cagggacctg tagtttgacc
     1861 actttgttct gttcgaacga cattgtaagc ctttaccgaa aacggtttaa taagtgtgta
     1921 aacaaaacag aacaaaggag agaataatta tggcaactga aacaagaatt ttggaccaaa
     1981 cagacgaaaa taagctgccg ctgagcgaga agctgacgtt cggcttcggg aacttggcgg
     2041 ccaatctgat gcttacgacc gccagttcct tcatcactta ttactatacc gatgtggtcg
     2101 gcctgagcgc agtcatcgtc ggcaacatcc tcttgtgggc acgacttttt gatggcgctt
     2161 ccgacttggc aatgggtgcg atcgtcgaca agacccgttc gaaacacgga aaggctagac
     2221 cttggatttt atggatggcg atcccttatg cattggcaat gatactctta ttcacttctc
     2281 cggatttcct gggatcgact ggcaaagccg tctatgcttt cgtgacttat gtgattgctt
     2341 tggctggtgt atacacagcg acgatggttc catataacgc aatgatcggt actacgacat
     2401 cgaacccagt cgatcgcggc aacctatcga catcacgcac cttagctgga tttgtggggg
     2461 cgatgggagt gactgcagcg gttttgccga tcgtaaactt cttcggtggc gataaacagg
     2521 cctggacatg gatggccgct cttttcgggg gtatctcgac agtgctgttg ttgattctgt
     2581 tcaaaaattc taaggaacgc gtcatcgaat ctgctgttgc tgtcgaaaag gttcctttga
     2641 aaaagaatct taaggcgctg ttgcagaata aatactgggt catcatgatt ctatatatgc
     2701 taatcagttt catcagctct ggtttgggtg gcatcaacat tttttatgcc caatggattt
     2761 tgggggatcc tgcaaaagtg gcagtaatcg gtattctttc ttttatgccg attgcggtag
     2821 gtgcagtgtt tatgcctgtt ctgttgagca aattcagcaa aaaaacgatc accttgatcg
     2881 gaggcatcgt aatgatgata ggtctgctga tcatcgcggt gttcccggaa aactttacga
     2941 tgattatggt cggtttgatc atccgcggac tcggtatcgc gccgggagct gtcgcaggct
     3001 tcgctatgtt gggtgacgtt gccgattacg gtgaatggaa aacgggcatc cgcagcgaag
     3061 gtttgatttt ctcggcagga acttttgccg aaaaagtcgg ctccggagtg ggcgggctga
     3121 ttctgggtgt cgttttagga cttggcggct atgtgtcgca aggggcaacg caatcaacag
     3181 aagctttatt cgcgatcaag gcaatcttcg cctatctgcc actcgctttc acagcaatct
     3241 gcattttgtt gattctgttc tatgacttgg acaaaaaact tcctgaaatt gcggaagact
     3301 tgaaagcgaa acgagtgaac ggataatgtt acaggctggg acaaaaaccc agcctgaaat
     3361 tatatatccg aatattattt gcgcaaacgt gcgccaaaag gcaggccctg gttccacttc
     3421 acgaaaaatg ctcagatggt ctgcgaccat cttgcgctta ttcgctccag tggtccgggc
     3481 ctaaacgcct tttgtcccac tctcattttt taagacagga gcacacacaa tgaatatata
     3541 taatctgaaa accaattacc taatgaatcc tatcggcttt ttgatggaca cggtggcttt
     3601 atcgtggata atcgaggcgc cgtttgagga ggaattgcag ggaatccgtg tcctcatcgc
     3661 actggatgaa aactttgaag aggtcatcca tgacagcagg atgcaagccg atttggattc
     3721 gctgtgctat cagccggaaa tcaagctgca gccgcgcacg cgctacttct ggaaagtgat
     3781 tgcgaagaat gttagtggag acgagtcttc ttccgaaatc cactacttcg agacggcgaa
     3841 ggaagaggaa ctgtggaccg gaaaatggat cgaacctgcc cgacaattgc aaaggatgcc
     3901 gcttgcgcgc aaactgttcc gcctgcctgc aaaacctatc cgtaaggcac gcgtttatgc
     3961 gacaggactg ggtatttatg aactgtatct gaacggagaa aaaatcggtg acgaatattt
     4021 ggctcccggc ttccattctt atgatttttg gcagcaatac cagacgtacg atgtcacaga
     4081 agcccttcaa gcaggtggaa atgcgatcgg cttcatgctg ggggacggct ggttcaaagg
     4141 ccgcttcgga ttcgacggtg gatatgaaga tctgtacggg gatcagttcc gcctgatcgc
     4201 ggaatgcatc gttacttatg aggacggaac ggaagaaaag ataacgaccg atgaaaca
//
LOCUS       DAOMUJ010000113         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5906_length_4227_cov_2.120566, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000113 DAOMUJ010000000
VERSION     DAOMUJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_4227_cov_2.120566"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..877
                     /locus_tag="PLT73_04025"
                     /pseudo
     CDS             1..877
                     /locus_tag="PLT73_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176932.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxic anion resistance protein"
     gene            1124..1372
                     /locus_tag="PLT73_04030"
     CDS             1124..1372
                     /locus_tag="PLT73_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRM19583.1"
                     /translation="MGFIWSLIVGGVLGALAGSFMGKDIPGGIIGNIIAGFVGSWLGT
                     TLFGAWGPEIGGFYIVPALVGAVILIFIVSFAMRSMKK"
     gene            complement(1535..1735)
                     /locus_tag="PLT73_04035"
     CDS             complement(1535..1735)
                     /locus_tag="PLT73_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRM19584.1"
                     /translation="MEQGKVKWFNGEKGFGFIEVDGKDDVFVHFSAIQGEGFKTLEEG
                     QDVEFEIVDGNRGPQAANVVKL"
     gene            complement(2014..>2655)
                     /locus_tag="PLT73_04040"
     CDS             complement(2014..>2655)
                     /locus_tag="PLT73_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM19585.1"
                     /translation="MRVNKYTYETEVVYIEKEDNLERRAMQTVFYEVLVKLAKLLTPI
                     IPHTTEEIWSFLKEEEQYAQLAELPESSIREDQAAILGQWGKFMDIRDAALKALEVAR
                     NEKTIGKSFEAHLSLFVDEETKDLFASLDANLEQLFIVSQLDINELTAAPAEAMQFDN
                     LAILVEHAHGETCERCRAIKEEVGTIEDAATLCGRCADIVKSEYPEALVPEEA"
     assembly_gap    2656..2665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2666..4227)
                     /gene="ileS"
                     /locus_tag="PLT73_04045"
                     /pseudo
     CDS             complement(2666..4227)
                     /gene="ileS"
                     /locus_tag="PLT73_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377345.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
BASE COUNT         1169 a          975 c          899 g         1174 t
ORIGIN      
        1 ccttgaacga tctgatgtat cgtttgaatg agccaaaccg gaagacctcc gggcagaaga
       61 caacaatgtc ttccgcaaga tcttcggcaa ggtaaaacaa tccgcttacg aaatgaccgc
      121 aaaataccaa aagatcggtg cccaaatcga taagatcgca atcaaactgg ataaagaaaa
      181 aaatagcttg ctcaatgaca acatcacgtt ggagcagcta tatcaaaaaa ataaggatta
      241 ttttgaagcc ttgaatatct atatcgcggc cggtgaggtg aaaatggagg atcttcagaa
      301 aaacgcaatc ccgaaagcga tccagactgc tgaggcttcc caaagccaaa tggatgtgca
      361 gatcgtcaac gacctgaaac agttccttga ccgacttgaa aaacgtaccc atgacttgcg
      421 tttaaccaga caaatgacga tccagcaagc gccacagatc cggttgatcc aaaacaccaa
      481 tcaagcgttg gctgagaaaa tccagtcctc gatccacact gccatcccgt tatggaaaaa
      541 ccaagtggcc atcgccctta cgctgctgcg ccaaaaggat gccgtaacgg cgcaaaggca
      601 agtatcgcag accacgaatg atctgatgcg caagaattcc gaaatgctga aaatatcagc
      661 catcgaaact gccaaagaaa atgaacgggg tgtgatcgat atcgaaacgc tcaaacagac
      721 acagcaggat ctgatcgaaa ctttggaaga gacgctgaaa atccagcaag aaggtcggat
      781 caaacgcaaa gaggctgaaa aagaactctc tatgatggag gacgatttga aagttaaact
      841 gttgaatatc caccaacagg aacaacaact ccactgatac cttagggctg cctcgtcttt
      901 tcggggcagc tctttattta tcctcttatc ctgatttttg ttctttgtga aactgagctt
      961 ttctcgcatc agatggctga ctcccacttc ctgttttcag aaaaactacc atctcgaggg
     1021 cttattattt gtaatgtgac tcattttata tataataaga gtatagttaa ttggaaataa
     1081 ctatattgtt actgcactcg tattagaaaa aggaggaaga gaaatgggct ttatttggtc
     1141 tttaattgtt ggtggcgtct taggagcatt agcaggatcg ttcatgggta aggatattcc
     1201 cggtggtatt atcggtaaca tcattgctgg tttcgtcggt tcatggttag gaacaactct
     1261 gtttggagca tggggtccag aaatcggagg cttctacatt gttcctgcat tagtcggagc
     1321 agtcatcctg attttcatcg tatcctttgc tatgagaagt atgaagaagt aacctgtcac
     1381 tacataaact aagcagacag ccgtctgttt tctcataaaa ccgttgtttg gcaatcctga
     1441 tatggattgc cgaacaacgg ttttctttgc atgtaaaaag cgccacagat aaactctggg
     1501 cgctttgaat tcgctaaggt taaaaagaat aggattataa ttttacaacg ttagctgctt
     1561 gagggccacg gttgccgtca acgatttcga attcaacatc ttggccttct tctaaagttt
     1621 tgaaaccttc gccttggata gctgagaaat gtacgaatac atcgtctttt ccgtcaactt
     1681 cgataaatcc aaaacctttt tcgccgttaa accattttac tttgccttgt tccataatcg
     1741 tgtaatacct ccaaaatttt cattggtaaa atgctaatac aaataaatac tatcatatta
     1801 gtacaaatta ctaatataat tataacgaat atctcatcta aattcaagag gaatttatgc
     1861 gctttcctta aaaactttaa cagtaatgga accatctttg atttacgctt aaaatcaaaa
     1921 ggagcacgca ggaatgccgt cagaagacgt cttcctgcgt actctttact tttcattcac
     1981 atgcccttac caattttgtt ccgagcgata aggttatgcc tcttctggca cgagtgcttc
     2041 tgggtattca gatttcacaa tatctgcaca acgtccgcaa agtgtagctg catcttcgat
     2101 cgttccgact tcttctttaa tggcacggca tctttcgcag gtttccccat gggcatgctc
     2161 tacaagaatg gcaagattat cgaactgcat cgcttcagct ggagcagccg tcaattcatt
     2221 gatgtcaagc tgtgaaacga tgaataactg ttccagattc gcatcaaggg acgcgaacaa
     2281 gtcttttgtt tcttcatcaa cgaataagga tagatgcgcc tcaaaggatt tcccgattgt
     2341 tttttcatta cgtgcaactt ccaatgcttt caaagctgca tcgcggatat ccatgaattt
     2401 tccccattga cccagaatcg ctgcctggtc ttcacggatg ctgctttccg gcaattcagc
     2461 cagttgggcg tattgttcct cttccttcag gaaactccag atttcttctg ttgtgtgtgg
     2521 gatgatcggt gtcaaaagtt tagccaactt aaccaatact tcgtaaaata cggtctgcat
     2581 ggcgcgtctt tccagattat cctctttttc gatgtagaca acttctgtct cataggtgta
     2641 tttgttcaca cgcatnnnnn nnnnngtaga caacatcttt cgcaaaatca agatagaatg
     2701 ccgacaaatc aactgtacag aagttcatga ttgtttgata gatggtcgag aactcatatt
     2761 cattataagc tgtcacacat gtttcaacca cttgatccaa gcgcgccatc aggtattgat
     2821 cgacactgcg cagctcttcg taggctacgg catgttcctt cggttggaaa tcttccgtat
     2881 tcccgatcaa gaaacgcatg gtgttgcgga ttttgcggta agtttcagaa acttgcttca
     2941 gaatatcgtc actgattctc acatcggatt cgtaatcgac actggacacc cataaacgga
     3001 tgatatcggc gcccatattc ttgacgactt tttcaggcag gacagtattg cctaatgatt
     3061 tgctcatttt gcgcccttct ccatccaata cgaaaccttg ggaaagaact gtcttgtatg
     3121 gcgcaacccc attgatcgca acgctggtcg tgatgcttga attgaaccaa ccacggtatt
     3181 gatcggagcc ttccaagtac atatcggcag ggaatgtcaa atcttctctt gcccgaagca
     3241 cagcttcatg cgaagatccg gaatcgaacc agacatccat gatgtccatt tctttggtaa
     3301 attccccgtt cgggctgcca ggatgcgtga acccttcagg cagcaggtct ttcgcttctc
     3361 tttcgaacca gacgttcgat ccgaattcgc cgatcaattt cgcagcgtgg tcgatcgttt
     3421 ccggcgtgat gacaggttct ccattttccg catagaagat cggcaacgga acgccccaga
     3481 cacgttgacg ggatcatgtt gaacaagcgg actttgccgg atgggtgcag ccactctaca
     3541 ttttcgattt cgtccagaat gtcttgacgg aatttatcga tggatgcgaa ccattgcggc
     3601 gttgcgcgat agatgaccgg tttctttgtg cgccagtcat gcggatagct gtggacgaag
     3661 aaatccagtt tcagcaaagc gcctttttcc gccaacaggt cggtgatcgg tttattggct
     3721 ttgtcgtaga acatgccctc gaaaccaggg gcttcagccg tatagcaacc tttgttgtcg
     3781 accggagata atacgtctag accatatttt ttgctgacaa ggtagtcatc ttcaccatgt
     3841 cctggagcgg tatggactaa cccggtaccc gcttcaagtg tgacatggtc gcccaccatg
     3901 accaaggact cacgatcata aaacggatgc tgaacggtca tgtactccaa atctgatccc
     3961 ttcagttctt gcaggacttc cacagattcc catccgatgg cttccttgac tgtgtccaac
     4021 aattcttttg cgacaacaaa ttttcttccg tcagcggaaa caaccacata atcgaaggca
     4081 gcatttgcgg aaatgcctaa gtttgccggc aaagtccacg gtgttgtcgt ccagatgatg
     4141 aaggcagtat cgttagcaag aaggcctttg ccatctttga cttggaaagc cacgtagatt
     4201 gaaggagatt ttacatcttt atattcg
//
LOCUS       DAOMUJ010000114         4195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5957_length_4195_cov_2.104589, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000114 DAOMUJ010000000
VERSION     DAOMUJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5957_length_4195_cov_2.104589"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT73_04050"
     CDS             <1..283
                     /locus_tag="PLT73_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM19586.1"
                     /translation="AGAAGVLVGIYYNSISPLMGVTPGLKAFVAAVFGGIGIIPGAMV
                     GGYVIGIIETLVSAYGGSMFKDAVVYLILIIILIVKPSGIFGKNTKEKV"
     gene            293..1279
                     /locus_tag="PLT73_04055"
     CDS             293..1279
                     /locus_tag="PLT73_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM19587.1"
                     /translation="MKQLNKTNLGWLILIALMVVVLFVGVSSNVISPFYQITLVTILI
                     NVILAIGLNLVIGFSGQFSLGHAGFMAIGAYSGAIIGDKIEGLPGFFAGLFVGMFISI
                     VVALIVGIPTLRLKGDYLAIATLGVAEIIRILILNMGDLTNGARGISGISFPFESNMS
                     FIMVLFLIVIVTSIITINYIHSSPGRATIAIREDEIAAESMGVNATTYKVIAFMIGAA
                     TASIAGSLYGTYFSVITPGDFTFQKSIDVLIIVVFGGIGSVTGTIVAAVVLGVLNTYL
                     QSFGELRMIFYSLAIIAIMVFRPGGLLGNREFTMSSLMKRFAKKDTAKKEVQ"
     gene            1279..2052
                     /locus_tag="PLT73_04060"
     CDS             1279..2052
                     /locus_tag="PLT73_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19588.1"
                     /translation="MSLLEVKELTKNFGGLAAVSNVDFAIEENELVGLIGPNGAGKTT
                     FFNLLTGVYVPTEGTIELEIDNKKVLLNGKAPYKITDLGLARTFQNIRLFKELTVIDN
                     VLIAMHSTMGESTFHSLLRTPTYYRKEAEMREKALELLAIFELEKKYDVLAKNLPYGE
                     QRRLEIVRALATDPKILFLDEPAAGMNPQETAELTALIRKIQKDFKITVVLIEHDMSL
                     VMNVCERIYVLEYGRLLAKGTPEEIQKNPAVIKAYLGGD"
     gene            2090..2654
                     /locus_tag="PLT73_04065"
                     /pseudo
     CDS             2090..2654
                     /locus_tag="PLT73_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2658..3311
                     /locus_tag="PLT73_04070"
     CDS             2658..3311
                     /locus_tag="PLT73_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HRM19589.1"
                     /translation="MDVQSYMSTDLVTIQPSTKIIEALDLMKEHKIHRLPVVQGETIV
                     GLITEGIIERNTPSSMTSLDMHEVNYLLNKTTVADIMEKRVVTINKEALLEEAAVTMR
                     ENGISVLPVVEPGNRLIGIITEKDIFSAFIDVLGYYTPGVRVVVNIHEDKKGVLEDLT
                     DIFSEENINIQQIATYRKMGFVQVVIQLESKDTDGIRARLEEHGYEIGSIIYKPERV"
     gene            3553..4089
                     /locus_tag="PLT73_04075"
     CDS             3553..4089
                     /locus_tag="PLT73_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19590.1"
                     /translation="MNNALITIVLYTIKEQYVSEKAFYANQLGISPQSWDRWKKGEHG
                     LKPENMQIISKLFTDYEWMLVQKVCRNAEILPEVAENPVREYQFLKYQVAKKWIATDL
                     ADFKWYTTDETVHDSEIHKPAITTLRLECNYDFWSYKDIIDLRLPSIIRHQIDSEKIN
                     LLEWFNENTPDTITKITE"
BASE COUNT         1190 a          933 c         1022 g         1050 t
ORIGIN      
        1 agccggagcg gcaggggtat tggtcggtat ctactataat tcgatttcgc cattgatggg
       61 tgtgacgccg gggctgaaag ccttcgttgc cgcagtattc ggcggcatcg gtatcatccc
      121 aggcgccatg gtcggcggct acgttatcgg catcatcgag acgctggtca gtgcttatgg
      181 cggatcgatg ttcaaagatg cagtcgtgta cttgatcctg atcatcatcc tgatcgtaaa
      241 gccttccgga atcttcggaa aaaatacaaa agagaaagtg taggtgataa tgatgaaaca
      301 attgaacaaa acaaatctag gttggctcat tcttatcgct ttgatggttg tcgttctgtt
      361 tgtcggcgtc agctcaaatg tcatttctcc cttctatcaa attacgttag tgacaattct
      421 gatcaatgtc atccttgcga tcggattaaa tctggtcatc ggattttctg gccagttttc
      481 tctgggacat gctggtttta tggcaatcgg agcctatagt ggcgccatca tcggtgataa
      541 gatcgaaggc ttgccaggtt tttttgcagg gttattcgtg gggatgttca tttccatcgt
      601 tgtggcgctg atcgtcggca ttccgacact ccgtctgaaa ggtgattatt tggctatcgc
      661 gactctggga gtggcggaaa tcatccgcat cctgattctg aacatgggcg atcttacgaa
      721 cggcgcacgc ggaatcagcg gcatctcttt cccgtttgaa tcaaatatga gttttattat
      781 ggtcctgttc cttattgtga tcgtaacgag catcatcacc atcaactaca tccacagcag
      841 tccggggcgt gcgaccattg ccatccgtga agatgagatt gcggctgaat cgatgggtgt
      901 caacgcaacg acttacaaag tcatcgcctt tatgatcggt gcggcaactg ccagtatcgc
      961 aggttctttg tacggcacat acttcagcgt aatcacgcct ggcgatttca ctttccagaa
     1021 atctatcgat gtgctgatca tcgtcgtgtt cggcggtatc ggcagcgtaa caggcacgat
     1081 cgttgcggcg gttgtgttgg gtgtcctgaa tacgtatctg caatcgttcg gagagctgcg
     1141 gatgatcttc tactcgcttg ccatcatcgc catcatggta ttccgtccag gcggattgtt
     1201 gggcaaccgc gaatttacga tgtccagttt gatgaagcgt ttcgcgaaaa aagatactgc
     1261 taagaaggag gtccaataat gagcctatta gaagtaaaag aattgaccaa aaacttcggc
     1321 ggtttggccg ctgtatccaa tgtcgatttt gcgatcgaag aaaatgaatt ggtcggtctg
     1381 attggtccca atggcgctgg taaaacgacc ttcttcaatc tgctgacggg cgtttacgtt
     1441 ccgacagaag gtacgatcga attggagatc gacaacaaaa aagtgttgtt gaacggaaaa
     1501 gcgccttaca aaattacgga ccttggctta gcgcgtacat tccagaatat ccgtctcttc
     1561 aaggaattga cggttatcga taatgtattg atcgcgatgc acagcacgat gggcgagagt
     1621 acgttccaca gcttgctgcg cacgccgacc tactaccgca aggaagccga aatgcgtgaa
     1681 aaagctttgg aattgttggc cattttcgaa ctggaaaaga aatatgatgt tctggcgaaa
     1741 aatcttcctt acggcgagca acgtcgattg gaaatcgtcc gcgccttggc gacggatcca
     1801 aaaatcctct ttttggatga gccggcagcc ggtatgaatc cacaagagac tgccgaattg
     1861 accgcgctga tccgcaaaat acagaaagat ttcaaaatca ctgtcgttct gatcgagcat
     1921 gatatgtcct tggtcatgaa tgtgtgcgaa agaatctatg tgttggaata cggccgtctg
     1981 cttgccaaag gaacaccgga agaaatccaa aaaaatccag ccgtaatcaa ggcttatcta
     2041 ggaggcgact aacgtgctga aagtaaaaaa tctatccgtg cactatggca tgatccaagc
     2101 catcaaagat atcagctttg aagtgaatga gggcgaaatc gtgacgttga tcggagccaa
     2161 cggagcaggg aagtccacca ttttgcgtac gatttccggg ccgccatgta ttcaaaggtt
     2221 tgaccgtaca ggaaaacctt gatttgggaa gcttcacccg caaagacaaa cagaaccttg
     2281 ccggtgatct ggaagcggta ttcgaacgtt tcccgattct gcgcgaaaga aaaaatcagg
     2341 atgcggccac tttgtccggc ggggaacaac agatgctggc gatgggccgc gctttgatga
     2401 caaagccgaa gctgttgctt ttggatgagc cttcgatggg attggcgccg attttcatca
     2461 aggaaatttt ccacatcatc caggatatcc agaaacaagg cacaacgatc ctcttgatcg
     2521 agcagaacgc caacgttgca ttgaagatcg cgaatcgcgg atatgtattg gagacgggaa
     2581 atatcgtatt gaccggaacc ggggaagaat tattagcaag tgacgaagtt cagaaagctt
     2641 acttaggagg ctaaacaatg gatgtacaaa gctatatgtc aacggattta gtaacgattc
     2701 aaccttcaac aaaaatcatc gaagctttgg atttgatgaa agagcacaag atccaccgtc
     2761 tgccggtcgt tcaaggcgag actatcgtag gattgatcac ggaaggcatc atcgaacgca
     2821 acaccccttc cagcatgacc agcctggata tgcatgaggt caactacctg ttgaacaaaa
     2881 cgacagtggc tgacatcatg gaaaaacgcg ttgtgaccat caacaaagaa gcccttttgg
     2941 aagaagcggc cgtaacgatg cgcgaaaatg gcatcagcgt tctgccagta gttgagccgg
     3001 gcaaccgtct gatcggaatc atcaccgaaa aggatatctt ctctgccttc atcgatgtgc
     3061 tgggctatta tacacccggt gtccgcgttg tcgtcaatat ccatgaagac aaaaaaggag
     3121 tcctcgaaga cctgacggat atcttctccg aggaaaacat caacatccaa cagatcgcca
     3181 cgtatcgcaa aatgggcttt gtccaagtcg ttatccaatt ggaaagcaaa gataccgacg
     3241 gcatccgcgc ccgactcgaa gaacacggct acgaaatcgg atccatcatc tacaaaccag
     3301 aaagagtata ggatgtgatg attcgcgttt agatacccgt tcgttggaac aaacgactac
     3361 cgttcagggt acctgcgatt cagaaccgtt ctaaatagag tctgatgaat cccgttcagc
     3421 aagcgaaacc atccaaataa tcaaaccgct cactccaacc aaaacggagc gagcgttttt
     3481 tttcaggcaa atcatttctt ttatcacgta ataagtgtat aattagaaca tgaaagattg
     3541 gaggttacat aaatgaataa tgcactaata acaattgtct tgtatacgat caaagagcaa
     3601 tatgtcagtg aaaaagcttt ttatgccaat caactaggga tctcgccgca gagctgggat
     3661 cgctggaaaa aaggagagca tggtttaaaa ccagagaaca tgcagatcat ttccaaattg
     3721 ttcaccgatt atgaatggat gcttgtccag aaagtttgcc gcaatgccga aattcttccc
     3781 gaggtagcag agaacccagt aagagaatat cagttcctga agtatcaagt cgctaaaaaa
     3841 tggatagcga cggatttggc cgatttcaaa tggtacacca ccgatgagac cgtccatgat
     3901 tcagaaatcc ataaaccggc gatcacgaca ctccggctcg agtgcaacta tgatttctgg
     3961 agctacaaag atatcatcga tctacgcttg cccagcatca tccgtcatca gatcgattcg
     4021 gaaaagatca atctattgga atggttcaat gaaaatacac cggataccat cacaaaaatt
     4081 actgaatgat agaacataaa acggaaagct gagatgatat tatcttggct ttccgtgtgt
     4141 ttcagcgtta agacttactt ttcagctgtg gattgacggc cagttgtgat aaaat
//
LOCUS       DAOMUJ010000115         4188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5965_length_4188_cov_2.474474, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000115 DAOMUJ010000000
VERSION     DAOMUJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5965_length_4188_cov_2.474474"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLT73_04080"
     CDS             <1..336
                     /locus_tag="PLT73_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRM19591.1"
                     /translation="GPYSQGTRKNGFIFSSGQLPIDMETKELVSDPAAATTASLNNVL
                     TIVKAGGGSLESIVKVNVFVKDINDFDAINGAYTAFFGEHKPARSLVQVAALPKDAVI
                     EIEAIAVKD"
     gene            complement(657..1595)
                     /gene="sdaAA"
                     /locus_tag="PLT73_04085"
     CDS             complement(657..1595)
                     /gene="sdaAA"
                     /locus_tag="PLT73_04085"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM19592.1"
                     /translation="MYNKIAELVKAAEESGKPISELMLLQEMAKSHRTREDIWEQMRN
                     NYHVMKAAVEKGLAGDGVFSPTGLTGGEAVKLRNYRQRGNTLSGDDLLIAVESAIATN
                     EVNASLGIICATPTAGSSGTLPGVMFAIKDKLGLTEEQMVQMLFCASGFGMVIANNAM
                     IAGAAGGCQAEVGSASGMAAAAAVEIAGGTPEQASEAMAMAISNLLGLVCDPVAGLVE
                     IPCVKRNAIGAVNALISADMALAGLTNKIPADDVIEAMRKVGKNLPASLRETGLGGLA
                     ATREGVRLKMMIFGQQLEIKPEWVDDQPPIKANPFE"
     gene            complement(1614..2294)
                     /locus_tag="PLT73_04090"
     CDS             complement(1614..2294)
                     /locus_tag="PLT73_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HRM19593.1"
                     /translation="MSHTFRSIFDIIGPVMVGPSSSHTAGALALGKAAAQLFEVKPKK
                     VTIHYYESFADTHLGHGTDFAILGGILGFAADDPMIPQSLKMAESQKIAVTFIEEIGD
                     SPVGHPNTATMVLERDKKKISVTGSSIGGGSIVLNHVAMDNFSMRLDAHLPIYFIHQN
                     TLQYHLYSEAEWQNYFAFKGYPLNDIRIFRESDTEWVVLYLDTIPTKEMIYEINELPF
                     IEKAILIS"
     gene            2494..3171
                     /gene="rpiA"
                     /locus_tag="PLT73_04095"
     CDS             2494..3171
                     /gene="rpiA"
                     /locus_tag="PLT73_04095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRM19594.1"
                     /translation="MELKELVGLKAAEYIKDGMTVGLGTGSTAYYFVKEIGRRVNEEG
                     LAITGVTTSIQTKELSESLGIPLKSVDEVTHIDITVDGADEISEDFQGIKGGGGALLF
                     EKIVADNSKEVIWIVDESKMVKHLGAFPLPVEVVRYGSIQLFNKFNEKGYNPEFRKTA
                     TGENYLTDEKNNIIDLHLGEILDPRALAEDLIHLTGVVEHGLFLDCVDTVLVGTKDGV
                     KVIEANR"
     gene            3252..>3592
                     /gene="deoA"
                     /locus_tag="PLT73_04100"
     CDS             3252..>3592
                     /gene="deoA"
                     /locus_tag="PLT73_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HRM19595.1"
                     /translation="MRMVDLIIKKQEGKALTKEEIRFIIAGYTDGSIPDYQMSAWAMA
                     VFFQDMTDEERMELTMAMAESGDQIDLSAIEGIKVDKHSTGGVGDTTTLVLAPLVASC
                     GVPVAKMSGRGL"
     assembly_gap    3593..3602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3603..>4188
                     /gene="deoA"
                     /locus_tag="PLT73_04105"
     CDS             <3603..>4188
                     /gene="deoA"
                     /locus_tag="PLT73_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HRM19596.1"
                     /translation="SIPGFHIEIDEKEFAAIVNENKVAVIGQSGNLCPADKKLYALRD
                     VTGTVNSLPLIASSIMCKKIAAGADAIVLDVKTGAGAFMKTEEDAKALAHAMVKIGNL
                     AGRKTMAIISDMSQPLGVAVGNALEVEVAIETLKGNGPKDLEALCIELGSQMVYLGGK
                     SADLAEARELVTENLRNGKAFEKFKVFVASQGGNP"
BASE COUNT         1074 a          983 c         1104 g         1017 t
ORIGIN      
        1 gggccttatt cgcaaggaac aagaaagaac ggattcatct tttcatccgg gcaattaccg
       61 atcgatatgg aaacgaaaga acttgtatcc gatcctgcag cagcgacaac ggccagcttg
      121 aacaatgtgc tgacgatcgt caaagcgggc ggcggatcgt tggaatcgat cgtgaaggtc
      181 aacgtgttcg tgaaggacat caatgatttt gacgcaatca acggcgcgta cactgcattc
      241 ttcggcgaac ataaaccagc ccgctctttg gtgcaggttg ccgcgttacc gaaagatgcc
      301 gtcatcgaaa tcgaagctat cgcagtcaaa gactaatact aataaagaag aacgggtgtc
      361 tcacttggag acacccgttc ttcttttaga taggatttag ttcaggatgt ccgattcttg
      421 cgcattatcg gacatcccgc aggacagtcg cgctctagtt gtccgattca tacgagatat
      481 cggacaacga tcgatctgaa cgtgtgcgag atgtccgatt catacgcatt atcggacatc
      541 ctgcagggca ttcgagttct agttgtccga ttcttacggg ttattagaca ttcttagcat
      601 ggccatagtt gccgcatgat gaatcgcagc tcaatcaatt agaaaagaaa cgtctgctat
      661 tcaaacggat tggccttgat cggtggctgg tcatcgaccc attccggctt gatttccagt
      721 tgctggccga agatcatcat tttgaggcgg acgccttcgc gggtggctgc gaggccgccc
      781 aagccggttt cgcgcaggga ggcgggaagg ttttttccga ctttgcgcat cgcttcgatc
      841 acatcgtcag ccgggatttt gttcgtcagt ccggccaagg ccatatcggc gctgatcagg
      901 gcattgaccg caccgattgc attgcgcttg acgcagggaa tctcgaccag tccggcaacc
      961 ggatcgcaga cgaggccgag cagattgctg atagccatcg ccatcgcttc cgatgcctgc
     1021 tccggcgtgc cacctgcgat ttcgacggcc gcagcggcag ccatcccgga tgcgctcccg
     1081 acttcggcct ggcaccctcc ggcagcaccg gcaatcatgg cattgtttgc gatcaccatg
     1141 ccaaaaccac tggcacagaa cagcatctgg accatctgct cttccgtcag acccagtttg
     1201 tccttgatgg cgaacatcac accgggcaaa gtccctgatg aaccggcggt aggggtggcg
     1261 cagatgattc ccaatgaagc attgacttcg ttggtggcga tggcgctttc tactgcaatc
     1321 agcaggtcat ctcctgaaag cgtattgccg cgttggcggt agttccgcag ctttacggct
     1381 tcgccgcctg tcaacccagt gggggaaaat acgccatcac cagccaaacc tttttcgact
     1441 gcggctttca tgacgtggta attgttgcgc atctgttccc aaatatcctc gcgcgtccgg
     1501 tggctcttgg ccatttcctg cagcagcatc aattcggaaa tcggcttacc gctttcctct
     1561 gcggccttca ccaattctgc aatcttatta tacaaagcgc tattccttct ttcttaactg
     1621 atcagtatcg ccttttcgat gaaaggcagt tcgttgattt cgtagatcat ttctttggtg
     1681 gggatcgtat ccagatagag gacgacccat tcggtatcac tctccctaaa gatgcggata
     1741 tcgttcagtg gatagccttt gaatgcgaaa taattctgcc attccgcttc gctgtaaaga
     1801 tgatattgga gcgtattctg atggatgaaa tagataggca gatgcgcatc cagacgcatc
     1861 gaaaagttat ccatggcgac atggttcaga actatcgaac cgcctccgat ggaactgccc
     1921 gtgacggaaa tttttttctt gtcccgctcc aacaccattg tggctgtgtt cgggtggcca
     1981 acaggagagt caccgatttc ttcgatgaag gtcacggcta tcttttggga ttcggccatc
     2041 ttcagggatt gcggaatcat cggatcatcc gccgcaaaac caagaatacc gccgaggatc
     2101 gcaaaatcag tgccgtgacc gagatgggta tccgcaaacg attcgtaata atggatcgtg
     2161 acttttttcg gtttgacctc gaacagttgc gctgccgctt ttcccaaggc cagggcacct
     2221 gcagtatgcg aactggaagg accgaccatg accggaccga tgatatcgaa aatgcttctg
     2281 aatgtatggc tcatgacgat ctcctcctcg ttatcggttg ataaaagctt gcttctcatt
     2341 atagcgaaaa ggctgtcgga atgacagaac accgcttcgc ttttttagcg gattctaatg
     2401 gctttccaac ggagtttccg ctggctaaga tagtaacgct tcctttccgt atgatataat
     2461 gatggcatca tcctgaaagg aacgcgtgag aaaatggaac taaaagaact ggttggatta
     2521 aaagctgcag aatacatcaa agacggtatg acagtaggtc tgggaacggg ctcgactgct
     2581 tattattttg tcaaagaaat cggtagacgt gtaaacgaag aaggactggc aatcaccggg
     2641 gtgacgactt caatccaaac gaaggaattg tctgaatcgt tagggattcc attaaaaagt
     2701 gtggatgaag tgacccacat cgacatcaca gtggacggag ctgatgaaat cagcgaagat
     2761 ttccaaggga tcaagggtgg cggcggagcc ttattattcg aaaaaatcgt tgccgacaat
     2821 tcaaaagaag tcatttggat cgtcgatgaa agcaaaatgg tgaaacatct tggcgcattt
     2881 cccctgccgg tggaagtcgt ccgctacgga tcgatccaac tgttcaataa attcaatgaa
     2941 aaaggctata atccggaatt ccgcaaaact gcaaccgggg agaattattt gactgatgag
     3001 aaaaacaata tcattgatct ccatctgggc gaaattctgg atccgagagc gcttgccgaa
     3061 gacttgatcc atcttaccgg tgtcgtggaa catgggttgt tccttgactg tgtggatacc
     3121 gttttggtcg ggacaaaaga cggcgtgaaa gtcatcgagg caaatagata agcttcagtt
     3181 taaggctata aaaaaacgat ttgcgttgcg tttgatattc gaaaagtcag actcagatgg
     3241 aggaaaagtc tatgagaatg gtagatttaa ttatcaagaa acaagaaggc aaagccttga
     3301 cgaaagaaga aatccgtttc atcatcgctg gctacacaga tggatcgatc cctgattatc
     3361 agatgagcgc atgggcgatg gctgtctttt ttcaggatat gacggatgag gaacgaatgg
     3421 aattgacgat ggctatggct gaatccggag accagatcga tctttccgct attgaaggca
     3481 tcaaagtcga taagcattca acgggcggtg tcggcgatac gacgacgctg gttttggctc
     3541 cattggtggc cagttgcggt gtgccggtag cgaaaatgag cggacgcgga ctnnnnnnnn
     3601 nnatcgatcc ccggtttcca cattgaaatt gatgaaaagg aatttgctgc catcgtgaat
     3661 gaaaataagg tcgccgttat cggacaatcc ggcaatcttt gcccagcgga caagaaattg
     3721 tatgcgctcc gggatgtgac cggaaccgtt aactcgttac cgctgattgc aagttcgatc
     3781 atgtgcaaaa aaatcgcagc cggggcggat gcgattgtgc tggatgtgaa aacgggagcc
     3841 ggtgcattca tgaagacgga agaagatgct aaggcacttg cccacgccat ggtcaaaatc
     3901 ggcaatttgg ccggccgcaa aacgatggcg atcatttccg acatgagcca gccgctgggg
     3961 gtagcggtcg ggaacgcctt ggaagtcgaa gtggctatcg aaacattgaa gggcaacggg
     4021 ccgaaagatt tggaggcgct ctgcatcgaa ttgggaagcc agatggtcta tctgggcgga
     4081 aaatcggctg atttggctga agcccgcgaa ttggtaaccg agaacctccg caatggcaaa
     4141 gcgtttgaga aattcaaagt ttttgtcgcc tctcaaggag ggaatcca
//
LOCUS       DAOMUJ010000116         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_5988_length_4171_cov_2.110544, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000116 DAOMUJ010000000
VERSION     DAOMUJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_4171_cov_2.110544"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLT73_04110"
     CDS             complement(<1..863)
                     /locus_tag="PLT73_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRM19597.1"
                     /translation="MKKLKLLILSLLSFSLVGCSFITTEKTSVSQQDAVGVDNVTTEI
                     SKMDGEVGAGLLIGEKVVTTVHLSYETLKYDDSIAYLKEIVAKYGAYVEYSYESSGGD
                     MFYTTSSLSQSYRQGNYTIRIPKDSVTAFLDDLEGGLGTKVSEQQGNEDVTQYYEDTT
                     TRISVLQRKEERLLALLDQAETVEEILAIEDSLSAAISEREVLQAELDNIDDLIDYTA
                     LYLTVSERSRISNNQGGSTPFWGRVKDAFTDSLYSFYYWLQDAAIWFIYALPFFVAVL
                     LLLLLLWVIKKL"
     gene            complement(1476..2123)
                     /locus_tag="PLT73_04115"
     CDS             complement(1476..2123)
                     /locus_tag="PLT73_04115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM19598.1"
                     /translation="MEKTTKRKLLFIGDSITDVGRNRNNPDSLGKGYVALIAKALADR
                     DVSERYQLINRGISGNRILDIAERWHADCVSLEPDVVTLLIGINDTWHNVGDEAVFAT
                     EAGAEQFEMHYRHLLASLRKKSDARLILMEPFVFPYPEDRKTWRVDLDPKQEIVKRLA
                     EEFDAEWIGLDAYLNAIGAIDGYETLSNDGVHPTKKGHQLIADAWLKQFDQGGKK"
     gene            complement(2133..2507)
                     /locus_tag="PLT73_04120"
     CDS             complement(2133..2507)
                     /locus_tag="PLT73_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19599.1"
                     /translation="MKMLHTCIRVQHLDKSIAFYKEAFDFREKKKLDYPEHKFSIVYL
                     GLEGDDYQLELTYNYDHEPYNIGDGYGHIAIGVDEFEALHDKQVAAGFNVTDYKGLPD
                     SSVRYYFITDPDGYKVEVIQNN"
     gene            2655..3395
                     /locus_tag="PLT73_04125"
     CDS             2655..3395
                     /locus_tag="PLT73_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19600.1"
                     /translation="MKICIYCHNRIPKEALICPMCGSDVSGSEAIPQDAFYQSTLFEE
                     STEHILDVTADDEAYSEATSRAEKQSFTFNEAKHKATRYIRFLWKKMKDPAETRKSRR
                     ESHNLYGYFTFVLSALLSAGLVTRIAVALTKQYDLLSQISLLPSVTFQIEPVVWFMKN
                     FLLFLTFYYLYPLLAYLIKTFFLHRRHALHNWMTQYAGMNAFGLLLLSAAFVISLIAP
                     ILMAIPVLLLFLVHLSGDIHRKPVPNNQ"
     gene            complement(3567..3965)
                     /locus_tag="PLT73_04130"
     CDS             complement(3567..3965)
                     /locus_tag="PLT73_04130"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRM19601.1"
                     /translation="MNSEELVSRARVAMQHAYSPYSHFPVGAAVLYKDGAVIEGVNVE
                     NVSFGATNCAERTALFTGVTRGYRKEDVAAIAIAGDTDTFLPPCSICRQVMVELCGYE
                     TPVYLSNKHGKIKDLTIKELVPYAFTELNM"
BASE COUNT         1035 a         1067 c          919 g         1150 t
ORIGIN      
        1 agttttttga ttacccataa aagcaacaac aacaatagaa cggctacaaa gaagggcagt
       61 gcatagatga accagattgc ggcatcctgc aaccaatagt agaaggaata aagcgaatcg
      121 gtgaacgcat ccttgacgcg accccagaaa ggcgtcgatc cgccttggtt attcgagatg
      181 cgcgaacgct cggaaacggt cagatacaaa gccgtatagt cgatcaagtc atcgatgttg
      241 tccaactcag cctgaagtac ttccctttcg gatatggcag cactgagact gtcttcgata
      301 gccaggatct cctcgactgt ttctgcttga tcgagcagcg ccaacaaacg ctcttctttc
      361 ctttgcagta cgctgatgcg cgttgttgtg tcctcgtaat attgcgtcac gtcctcattg
      421 ccctgctgct cgctgacttt tgtgcccaag ccgccctcca gatcatccaa gaaggccgtc
      481 acggaatctt tcgggatcct tatcgtgtaa ttgccttggc gatagctttg actcaatgaa
      541 gaggtagtat agaacatgtc gccgccgctg gattcgtagg agtactcaac ataggccccg
      601 tacttagcga caatttcctt cagataagcg atggaatcat catatttcaa cgtttcatag
      661 gaaaggtgga cggtggtcac taccttttcg ccgataagca aacccgctcc tacttctcca
      721 tccattttgg atatttcggt cgtcacgttg tctactccga cagcgtcctg ttgcgaaaca
      781 ctcgtttttt ccgtcgttat gaatgagcaa cccaccagcg agaatgacag cagcgatagt
      841 atcaataatt tcagtttttt caattgaggc gcctccaatc tttcctgaat ttcctactta
      901 gattataccg caatgaggcc gcaccgccta ataaagccct tgcaggtttg gggtccggct
      961 gtggttgatt tagttggctt ggctggagaa catctgaaga aatcgaagga ccgagcgctt
     1021 ctccggtaaa gcggccttta tcggaggtgg taagactttg acgttccctc ttcctgcgaa
     1081 gcgatcttcg cagaaggagc ggttctttgg cggtcgtaag ctccggtgaa ccactcctcg
     1141 ccggagctga cggcccccgc cccactactg aaaccacaaa aagaaagggc cggaaaggca
     1201 aacacctaag tgttcatctt tccgacccat tcttctgata cggcatgggt aaacccatgg
     1261 atgggactgt tcacagcagt caccatcaaa catccacatc ctgtggttgt gcaagcacgt
     1321 atgtcccgtt tgatttgcaa tcacttcgcc gcttgcgcgg aatgggcgaa cccacctgat
     1381 tgctcgtcgc ctcatcgcat gtaaaacatc ttatcatgca gaaaccaaga aagcaagtct
     1441 tttcttttag ttgcatacac ctgacgaact ggcccttact ttttgccccc ctggtcaaat
     1501 tgcttcagcc aagcatcggc gatgagctgg tggccttttt tggtcggatg gacgccgtca
     1561 ttcgataatg tttcatagcc atcgatagca ccaattgcat tcagataagc atccaaccca
     1621 atccattcag catcgaattc ttcggctagg cgtttgacga tttcctgttt cggatccaaa
     1681 tcaacccgcc aagttttgcg gtcttccgga taagggaaga caaagggctc catcagaatg
     1741 agccgtgcat ccgacttttt ccgcaaggag gccagcaagt ggcggtaatg catctcgaac
     1801 tgctcagccc ccgcctcggt cgcgaacacc gcttcatcgc cgacattgtg ccacgtatca
     1861 ttaatgccaa tcaacaaagt gacgacatcg ggttcgagcg aaacacagtc tgcgtgccat
     1921 ctttccgcta tgtccaagat tcggttcccg ctgatgccgc ggttgatcag ctggtagcgt
     1981 tcggaaacat ccctatccgc aagagccttc gctatcaaag ctacatagcc ttttcccaac
     2041 gaatcaggat tgtttcggtt gcggccgacg tcggtgatgc tgtcgccgat gaaaagcaat
     2101 ttccgcttgg tcgttttctc cataaggccc tcctaattgt tctggatgac ttcgacctta
     2161 tagccatccg gatcggtgat gaagtaatat cttactgaag aatcaggcaa acctttatag
     2221 tcggtcacat tgaatccggc tgccacctgt ttgtcatgaa gggcttcaaa ttcgtccacc
     2281 ccgattgcga tatgtccata accgtcaccg atgttgtaag gttcgtgatc gtaattgtag
     2341 gtcagctcca actgataatc atcgccttcc aagcctaaat agacgataga aaatttgtgc
     2401 tcagggtaat ccagcttctt tttctcgcgg aaatcaaaag cctctttgta gaatgcaatc
     2461 gatttgtcca agtgctgcac gcgaatgcaa gtatgtaaca ttttcattat gctgtcattc
     2521 ctctcattta tactgtcttt atcataccga attattgcct ccaatacaaa atgtttgtcg
     2581 gaaaaataac cttgctatat ccaacaaact gaattattct agaagttagt tactagcgag
     2641 aaggagaaaa tccaatgaag atatgtatct attgccataa tcgtatcccg aaagaagctt
     2701 taatctgtcc catgtgcggg tcggatgttt ccggttcgga agcgatccct caagatgcgt
     2761 tttatcagtc cactttgttc gaggagtcta cagaacatat tttagatgtt acggctgatg
     2821 atgaagcgta ctcagaagcg acttcgcgcg ctgaaaaaca atccttcact tttaatgagg
     2881 ccaaacataa agccacccgc tacatccgtt ttctgtggaa aaaaatgaaa gaccccgcgg
     2941 agaccagaaa atcacgcaga gaaagtcaca acctttacgg ttacttcacc ttcgtccttt
     3001 cagcattgct ttcggccgga ttagtgacgc gcattgcagt tgcattgaca aaacaatatg
     3061 acctgctttc gcagatttcc ttgttgccat ccgtcacttt tcaaatcgaa ccagttgttt
     3121 ggttcatgaa gaatttcctg ctgttcctga cattctatta tctctatccc ctgctggcgt
     3181 atctgatcaa aacattcttt ttgcacagac gtcatgcctt acacaactgg atgacgcaat
     3241 atgcgggaat gaacgctttt ggcttgttgc tgttaagcgc tgcgtttgtg attagcctga
     3301 ttgccccaat tttgatggcg ataccggtgc ttttactgtt tttggttcat ctatctggtg
     3361 acattcatcg caagcctgta ccaaacaatc aatgaaacca aacgggatac cctttacctt
     3421 tgcctcggcg gcatcagcgc tcatttgctg ctggtgatga cgttcgccta cttgctgttt
     3481 ctgaaaatgt aggatcacaa agcaacgcat ccgctccaaa acaaaacaac tgctgcccac
     3541 cttggactgg tgggcagcag ttgtttttac atatttaatt ctgtaaaggc atacggaacc
     3601 agctctttga tcgtcagatc cttgattttc ccgtgcttgt tggatagata gactggtgtt
     3661 tcatagccgc acaactccac catcacctgt cggcagatac tgcacggtgg aaggaatgta
     3721 tctgtgtcgc ccgctatggc gatggcagct acatcctctt tacggtagcc tcgtgtcaca
     3781 cctgtgaata aggcagtccg ttctgcgcag ttggtggcgc cgaaggaaac attttccaca
     3841 tttacgcctt caatcactgc cccgtccttg tagaggactg ccgcccctac cggaaaatgc
     3901 gaatacgggc tgtatgcatg ctgcatggcg actcttgctc tggacactaa ttcttcactg
     3961 ttcatcgacg ttccctcact tgtcatatat gggtattttt ctgacggtaa atgttcgtgt
     4021 ttacagtata ccgattattt cagaatttgc caaatcgaaa agaccagcaa cccggttaaa
     4081 acagtggacg ttcggatact gagcgcttgg atcaaagttt cggcaaattc gccatgtact
     4141 tatcaagtgc ttgaattgct tccgccatca a
//
LOCUS       DAOMUJ010000117         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6026_length_4149_cov_2.221788, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000117 DAOMUJ010000000
VERSION     DAOMUJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6026_length_4149_cov_2.221788"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..>1645)
                     /locus_tag="PLT73_04135"
     CDS             complement(65..>1645)
                     /locus_tag="PLT73_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19602.1"
                     /translation="SISQGIQKKIRDELFAHMQTLPIKYFDTHAHGDVMSRYTNDIDT
                     LRQMLSQSIPMAFSSLVTIVSVFAAMLAVSLPLTLIVIVMVALSITVTKTIGGKSSVN
                     FVKQQQTLGKVNGYIEEMMHGQKEVKVFGRETESKERFDNLNDELRESATNANIYANI
                     LMPILGNLGYLQYALIAMFGGAMAVSGIGGLTLGAIASFLQLSRSFTMPVNQISQQVS
                     SIVMALAGAERIFELMDEKPELDEGTVTLVNVTMVGNELMEAENHTGLWAWKVPESDG
                     SVRYTQLTGDVRFFDVDFSYNPDKQILHDISLYAHPGEKVAFVGSTGAGKTTITNLIN
                     RFYDIQDGKIVYDGIDIADIKKNDLRRSLGIVLQDTNLFTGTILENIRYGNLHASDED
                     VYAAARLANADDFISRLPLGYQTVISGDGEGLSQGQKQLLSIARAAVADPPVMILDEA
                     TSSIDTRTESIVQRGMDALMQGRTVFVIAHRLSTIQNADVIMLMEQGRIIERGDHEKL
                     IGEQGKYYQLYTGAFELE"
     assembly_gap    1646..1655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1656..1949)
                     /locus_tag="PLT73_04140"
     CDS             complement(<1656..1949)
                     /locus_tag="PLT73_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19603.1"
                     /translation="MKPNQPKRRGAKDAGKTTKRLLSYISKGHKFTFSLVLICIIISA
                     LANVAGSLFLETLIDDYITPLLGVSNPVFTSMYRSIALMAGIYLIGVVATYVYN"
     gene            complement(1939..3678)
                     /locus_tag="PLT73_04145"
     CDS             complement(1939..3678)
                     /locus_tag="PLT73_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM19604.1"
                     /translation="MIKKLLGSIREYKKDSILAPIYVTLEVVLEVLIPFLMSMIIDRG
                     VGEGDMPFIIRIGLILIVSTLFSLIFGALSGLHAAKASAGFAKNLRKDMYYNIQDFSF
                     SNIDQFSTSSLITRLTTDITNVQNSYQMIIRIMVRAPLMLVFSLMMALNINSELGMVF
                     LGAIPFLGIGLYLIMSQAFPIFQRVFRTYDKLNRVVQENLYGIRVVKSYVREEHETEK
                     FKSVSKKIYTDFTKAEKILAFNSPLMQFTMYASILTLSLLGARMIVSGSMTTGQLMSL
                     ITYASQILMSLMMISMVFVMVTISRASAERIVEVLSEESDLKNSDNPVMVIPDGSVSF
                     ENVSFSYSKDSNKLALKDVSFSVKAGETVGIIGGTGSAKTSMVHLIPRLYDVTGGTVK
                     VGGIDVRDYDMPALRSAVAMVLQKNILFSGTIKDNLRWGNPFATDEELMIAARQAQAD
                     EFIQRLPDGYDTYIEEGGTNVSGGQRQRLCIARALVKQPKILILDDSTSAVDTRTDSL
                     IRTAFREELPNTTKFIIAQRVSSVQDADKIIVMESGSINGIGTHDELLASNTIYQEVY
                     DSQTKGGQLADEA"
     gene            complement(3735..>4149)
                     /locus_tag="PLT73_04150"
     CDS             complement(3735..>4149)
                     /locus_tag="PLT73_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM19605.1"
                     /translation="RIRSVWQQVKRLMNRHLTENDGYGLTGMQFAIVSFIAKESTERD
                     VFQKDLEQKFDIRKSTVTGILNTMERDGLLLRETVPYDARLRKMILTDKALQAKKNSE
                     QVIDTVENQLSKGLTEEEIATFLTILEKISKNAEG"
BASE COUNT         1030 a          976 c         1013 g         1120 t
ORIGIN      
        1 cgattaggtc acagtgtttc cttaatcagg gctgaacgaa cccgttccgc ttttcttaaa
       61 agatttattc gagctcgaat gctcctgtat aaagctgata atatttccct tgctcgccga
      121 tcaatttctc gtgatcgccg cgttcgatga tccggccttg ttccatcagc atgattacat
      181 ctgcattctg gatcgtggac aggcggtggg cgatgacgaa aacagtccgt ccttgcatca
      241 acgcatccat gccgcgctga acaatcgatt ccgtccgcgt atcgatgctg gaggttgctt
      301 catccaagat catgacggga ggatcggcaa cggctgcacg agcaatcgaa agcaattgct
      361 tttggccttg agaaaggcct tcgccgtcgc cggaaatgac ggtctggtat cccagcggca
      421 gacgggagat gaagtcatcg gcattcgcca aacgcgctgc cgcataaacg tcttcatcgg
      481 atgcgtgcag attgccgtaa cggatatttt ccagaatcgt tccggtaaag aggttggtgt
      541 cctgtaacac gataccaagg gaacggcgca ggtcattttt cttgatgtcg gcgatgtcga
      601 tgccatcata aacaattttt ccgtcctgga tgtcatagaa acggttgatc aggttcgtga
      661 tggtcgtttt gccggctccc gtcgatccca caaaagcgac tttctcgccg ggatgggcat
      721 aaaggctgat gtcgtgcaga atctgcttgt cgggattgta ggagaagtcg acgtcaaaga
      781 atcggacatc cccggtcagc tgggtatagc ggaccgaccc gtcggactca ggcactttcc
      841 atgcccaaag tccggtatga ttttcggctt ccatcagttc attaccgacc atggtcacat
      901 ttactaaggt tacggtgcct tcatctaatt caggcttttc atccatcaat tcaaagatcc
      961 gctcagcccc tgccaatgcc atcacgatgg atgaaacttg ttgggaaatc tgattgaccg
     1021 gcatcgtgaa ggaacggctc aactgcagga aggaagcgat cgcacccaat gtcaggccgc
     1081 cgatgcccga gacagccatg gcgcctccga acatggcgat cagagcatat tgcaagtatc
     1141 ctagatttcc caggataggc atcagaatat tcgcatagat atttgcattc gtcgcactct
     1201 cgcgcaactc gtcgttgagg ttatcgaaac gttccttgga ttccgtttcg cgtccaaaaa
     1261 cttttacttc tttttgccca tgcatcattt cctcgatata gccgttcact ttaccgagtg
     1321 tctgttgctg cttcacgaag ttgacggagc ttttgccgcc gattgtcttc gtgacggtga
     1381 tcgataaggc gaccatcacg atgacgatga gcgtcaacgg aagactgact gcgagcatcg
     1441 cagcgaatac gctgacgatc gtaaccagag aagaaaaggc cattgggata ctttgtgaaa
     1501 gcatctggcg taaagtatcg atatcgttgg tatagcggct catgacatcc ccgtgcgcat
     1561 gcgtatcaaa atatttgata ggcaaggtct gcatgtgcgc gaacagctca tcgcggattt
     1621 ttttctggat accttgtgaa atggannnnn nnnnnattgt agacataggt tgccacaaca
     1681 ccaatcagat atattccagc catcagcgcg attgaacggt acatactagt gaagaccgga
     1741 ttggatacgc ccaataatgg cgttatataa tcatcgatca gcgtctcaag gaaaagcgag
     1801 cctgctacgt tagcgagggc gctgatgatg atacagatca atacaagact aaatgtgaat
     1861 ttatgacctt tggaaatata ggacaggagg cgttttgtag ttttgccggc atctttggcc
     1921 ccacgccgtt ttggttgatt aggcttcatc agcaagttga cctccttttg tttgtgaatc
     1981 gtacacttct tggtaaatgg tgttcgaggc aagcagttcg tcatgcgttc cgatgccgtt
     2041 gatactgccg ctttccatca cgataatttt gtcggcatcc tgcacggaag agacgcgttg
     2101 ggcaatgatg aatttggttg tgttcgggag ttcctccctg aaagcagtcc ggatcaaaga
     2161 atccgttcgg gtatccactg cgctggtcga atcatccaga atcaggatct tcggttgctt
     2221 caccaatgca cgcgcaatgc agagcctttg tctttggccg ccggaaacat ttgtaccacc
     2281 ttcttctata taggtgtcgt agccatccgg taggcgttgg atgaattcat cggcttgtgc
     2341 ctgtctggct gcaatcatca gctcttcatc ggtagcgaaa gggtttcccc aacgcagatt
     2401 gtctttgatc gttccggaaa acaggatatt tttttgaagc accatagcga ctgcgctgcg
     2461 caaagcaggc atatcataat cacgtacatc gataccgccg actttcaccg taccgccggt
     2521 gacatcatat agacgcggaa taaggtggac catagaggtt ttggcactac cggttcctcc
     2581 gatgatgcct acggtttctc ctgctttgac ggaaaaactg acatccttca aagcaagttt
     2641 attcgaatct ttgctataac tgaaactcac attttcgaag gagacagatc catcgggaat
     2701 gaccataaca ggattatcgc tgtttttcag atcgctctct tcgctcagca cttccacaat
     2761 ccgttccgct gaagcacggg aaatcgtgac catgacgaat accatcgaaa tcatcatcag
     2821 actcatcaga atttgggaag cgtaagtgat gaggctcatc aactggcctg tcgtcatcga
     2881 accggagacg atcatacgcg caccgagtag agacaatgtc aaaatgcttg catacatcgt
     2941 gaactgcatc aaaggactgt tgaacgccaa tattttttca gcctttgtga aatcagtgta
     3001 gattttcttg gagacggatt tgaatttttc agtttcgtgc tcttcacgga cataggactt
     3061 gacgacccgg atgccataaa gattttcctg gacgacacga ttcagtttgt cataggtccg
     3121 gaacaccctt tggaaaatcg ggaaggcttg tgacatgatc aggtagagac cgataccaag
     3181 gaacgggatc gctccaagga agaccatccc aagttcacta ttgatgttca aagccatcat
     3241 caatgagaac acgagcatca gcggcgctcg gaccatgatc ctgataatca tctggtaaga
     3301 gttctggaca ttggtgatgt ctgtcgtcaa acgggtgatc agactggatg tcgaaaactg
     3361 atcgatattc gaaaatgaaa aatcttggat gttgtagtac atgtccttgc ggagattttt
     3421 tgcgaatccg gcggaagctt tggccgcatg caatccggat agggctccaa aaattagtga
     3481 gaagagggtt gagacgatca aaataagtcc gatgcggata atgaagggca tatccccttc
     3541 gccgacgcct cggtcgatga tcatagacat cagaaatggg atgagcactt caaggacgac
     3601 ttccagggtg acatatatag gggcgaggat tgaatccttt ttatactcgc gtatgctgcc
     3661 caaaagtttt tttatcatag cgttaatcct ttccgtaaat aatgattggg tgttcttgga
     3721 aattgttgag gcaatcagcc ttctgcattt tttgagattt tttcgagaat cgtcaaaaaa
     3781 gtggcgattt cttcttcagt taaacctttg gataattggt tctccacggt atcaatcact
     3841 tgttctgaat ttttttttgc ttgcaaagcc ttatccgtca gtatcatttt ccgcagtctg
     3901 gcgtcgtatg gaacggtttc ccgcagcagc aagccatccc tttccatcgt attcaggatg
     3961 cctgtgacgg tcgatttgcg gatgtcgaat ttctgctcga gatctttctg aaagacgtct
     4021 cgctctgtgg attctttagc gatgaaggaa acgatcgcga attgcatgcc ggtcaggcca
     4081 taaccatcat tttcggtcaa gtgcctgttc atgaggcgct tgacttgttg ccaaaccgac
     4141 ctgatccgg
//
LOCUS       DAOMUJ010000118         4139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6047_length_4139_cov_1.955926, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000118 DAOMUJ010000000
VERSION     DAOMUJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_4139_cov_1.955926"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLT73_04155"
     CDS             complement(<1..816)
                     /locus_tag="PLT73_04155"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HRM19606.1"
                     /translation="MNKLLVFGHQNPDTDAITSAIAYAYYLNHNGTEAEAVALGLPSE
                     ETIYALNQFKIDAPRVIETAANETDTVALVDHNEAQQSVSDIKDVEVYAVVDHHRIAN
                     FETANPLYYRAEPVGCTNTILYKMFQEKNIAIPADIAGLMLSAIISDTLLFKSPTCTP
                     EDEAAASALTEIAGVSLQEYGLAMLKAGTNLDDKSIAELLTLDAKSFPMGAKNVRIAQ
                     VNTVDVQDVLNRKDELVAAMETENAVQGYDVFLLVVTNIIDSDSALLAVGSHLD"
     gene            complement(917..1678)
                     /gene="pflA"
                     /locus_tag="PLT73_04160"
     CDS             complement(917..1678)
                     /gene="pflA"
                     /locus_tag="PLT73_04160"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRM19607.1"
                     /translation="MTEPLKGFVHSTESFGSVDGPGIRFIIFMQGCRMRCQFCHNPDT
                     WNIGGGNEMTSDELLDMALQYKPFWGQKGGITVSGGEPLLQIDFLIELFTKAKAQGVH
                     TTLDTCGLPFTYDEPFFSQFNKLLAVTDLILLDIKHIDSQKHKELTMWPNESIIELAK
                     YLSEINKPIWVRHVLIPERTDYDEFLIRLDAFLKTLKNIVKVEVLPYHKMGVYKWQTL
                     GIPYQLEGIDPPTAERVENARKLLHTENYKGYLDL"
     assembly_gap    1776..1785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1812..>2887)
                     /locus_tag="PLT73_04165"
     CDS             complement(1812..>2887)
                     /locus_tag="PLT73_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM19608.1"
                     /translation="IQYENDDIMSLEWGDDYGIACCVSAMRIGKQMQFFGARANLAKT
                     LLYAINGGVDETKKKQVGPKLQPITSEYLDYDEVAEKFDVMMDWIVALYVNTLNVIHY
                     MHDKYSYESLEMALHDTHVVRTLATGVAGFSVTIDSLSAIKYAKVKPIRDENGIAIDF
                     EIEGDFPKYGNNDDRADQIGIDLLKQYMGKVKKHQTYRNSIPTTSILTITSNVVYGKK
                     TGSTPDGRKAGEPFAPGANPMHGRDTHGALASLSSVAKIPYKYSLDGISNTFSIIPKA
                     LGREEEVQKDNLATMLDGYIAKGGHHLNVNVFNRETLLDAMDHPENYPQLTIRVSGYA
                     VNFIKLTREQQMDVINRTMHEMM"
     assembly_gap    2888..2897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2898..4045)
                     /locus_tag="PLT73_04170"
     CDS             complement(<2898..4045)
                     /locus_tag="PLT73_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM19609.1"
                     /translation="MEQWQGFKGKTWKEEVDVRDFIINNFSEYTGDESFLAGPTETTT
                     QLWDQVMDLTKQEREAGGVLDMDTKVVSSITSHAAGYLNKEKEQIVGFQTEKPLKRSL
                     MPFGGIRMSEQACEEAGYKVDPEVSMIFRDYRKTHNQGVFDAYTPEMRAARTTGVITG
                     LPDAYGRGRIIGDYRRVALYGVDYLMQDKLKEFAKIGDGTMTEEIIRLREEFSEQYRA
                     LKELKELGNIYGFDISKPATNAKEAFQWLYFGYLAAVKQQNGAAMSLGRTSTFLDIYI
                     QRDLENGTLTEEEAQEIVDHFVMKLRMIKFARTPEYQELYSGDPQWVTEAIAGMAHDG
                     RSMVTKSSFRFLNTLTNLGAAPEPNLTVLWSTKLPEGFKKYCAKMSIET"
BASE COUNT         1033 a          921 c          877 g         1288 t
ORIGIN      
        1 gtccaaatgc gagcctacag ccaataaggc cgaatcgctg tcgatgatgt ttgtcaccac
       61 caacaagaag acgtcgtaac cttgaacagc gttttctgtt tccatggcag cgaccaactc
      121 atcttttcgg ttcagcacat cttggacgtc gaccgtgtta acctgtgcga tgcgcacgtt
      181 tttagcgccc atcgggaatg attttgcgtc caaagtcaac aattctgcga tcgacttgtc
      241 gtccaaattc gttccggctt tcagcatcgc caagccatat tcctgcaaag agacaccggc
      301 gatttcagtc aaggctgatg ccgctgcttc atcttccggc gtgcaagtcg gagatttgaa
      361 caatagcgta tcggaaatga tggcagaaag catcaagcca gcgatatcgg ctgggatcgc
      421 gatattcttt tcctggaaca ttttatacag gattgtattt gtgcaaccga caggctctgc
      481 acggtaatac aacgggttgg ctgtttcgaa gttcgcgatg cggtggtgat ccactacggc
      541 atagacttca acatccttga tgtcactgac gctttgctgc gcttcgttat gatcgaccaa
      601 agcgaccgta tccgtttcgt ttgcagcagt ctcgatgaca cgtggggcgt ctatcttgaa
      661 ttgattcagc gcatagattg tttcttcgct tggcagacct agagccacag cttctgcctc
      721 ggttccattg tgattcagat agtaggcgta tgcgatagcg gatgtaatcg catccgtatc
      781 cggattctgg tgtccaaaaa ctaatagttt gttcatttaa ttcactctct cttccattat
      841 cttaatttat cataataaaa aaggaagaaa agagcaatgg caattctctt ttctcccttg
      901 aaaaaatagt attattttac agatccaaat aacctttgta attttccgta tgcaacagtt
      961 ttcttgcatt ctcgactcgt tctgcggttg gcggatcgat tccctccaac tgataaggga
     1021 tgcccaatgt ttgccactta tagacgccca ttttatggta aggcagaacc tctactttga
     1081 cgatgttttt caatgttttc agaaaagcat ccaaacggat caagaattca tcgtaatcgg
     1141 tacgttccgg aatcagaaca tgacggaccc aaatcggttt gttgatttcc gacaaatatt
     1201 ttgccagttc gatgatgctt tcattcggcc acattgtcag ttctttgtgt ttctgactat
     1261 cgatgtgttt gatgtccagc agaatcaaat ccgtcacagc caataattta ttgaattggc
     1321 tgaagaatgg ttcatcataa gtgaacggca agccacaagt atccagtgtt gtatgtacac
     1381 cttgcgcttt ggctttcgtg aataattcaa tcaagaagtc gatctgcagc aacggttcgc
     1441 ctccgctgac ggtaatgccg cctttttgcc cccagaatgg cttgtattgc agagccatat
     1501 cgagcaactc atcacttgtc atttcattgc cgccaccgat gttccatgtg tccgggttgt
     1561 ggcagaattg gcaacgcatg cggcagcctt gcataaatat aataaaacgg attccgggtc
     1621 catcaacgga accgaaactt tctgttgaat gcacaaatcc ttttaaaggt tcagtcattt
     1681 cgaaaactcc tctatcattt atttcttaac ctcattgtat cttttataag gaatattttc
     1741 tatcaaaaag agcgattttt cgctcccttt gatagnnnnn nnnnnacgat taatatgcag
     1801 ggtgaaacaa attacatcat ttcgtgcatt gtacggttga ttacgtccat ttgttgttca
     1861 cgagtcaact tgatgaagtt aacagcgtat ccggaaacac ggatcgtcaa ttgcggatag
     1921 ttttcaggat gatccattgc atcaagcaat gtttcacggt tgaatacgtt gacgttcaag
     1981 tgatggccac ctttagcgat gtatccatcc aacattgttg caaggttgtc tttttgcact
     2041 tcctcttcgc gtcccaatgc tttagggatg atcgagaatg tattggagat accgtccaat
     2101 gaatatttgt aagggatctt agcaactgaa gacaagcttg ccaaagcgcc gtgtgtatct
     2161 cttccgtgca ttgggttagc accaggtgcg aatggttcgc cggctttacg tccgtctggc
     2221 gtgctaccag ttttcttacc atataccacg tttgaagtga tcgttaggat agaagttgtc
     2281 gggattgagt tgcggtatgt ttggtgtttt ttaactttgc ccatgtattg tttcaacaag
     2341 tcgataccga tttggtcagc gcgatcatca ttgttaccgt atttagggaa gtcgccttcg
     2401 atttcgaagt caatcgcaat gccattttcg tcgcggatag gtttaacttt cgcgtatttg
     2461 atagctgaaa gtgaatcgat cgtcactgag aaaccagcga caccagtcgc caatgtgcgg
     2521 acaacgtgtg tatcatgtaa cgccatttct aatgattcgt aagaatattt atcgtgcata
     2581 tagtggataa cgttcaatgt attcacatac aaagctacga tccagtccat cataacatcg
     2641 aatttctcgg caacttcatc gtaatcaagg tactctgaag tgattggttg caatttaggt
     2701 ccgacttgtt ttttcttcgt ttcgtcaacc ccaccgttga tagcgtaaag caatgttttg
     2761 gccaagttag cacgcgctcc gaagaattgc atttgtttcc cgattctcat cgcggaaacg
     2821 cagcatgcga tgccgtagtc gtcgccccat tcaaggctca tgatgtcatc attttcgtat
     2881 tggattgnnn nnnnnnngtt tcgatagaca ttttagcaca gtatttcttg aatccttcag
     2941 gcaatttagt tgaccaaagg actgtcaagt taggttctgg agcagctccc aagttagtca
     3001 atgtgttcaa gaaacggaag cttgattttg taaccattga gcgtccgtca tgtgccatac
     3061 ctgcgattgc ttcagtaacc cattgaggat cccctgagta caattcttga tattctggcg
     3121 tacgtgcaaa tttgatcata cgtaatttca ttacgaagtg gtcaacgatt tcttgtgctt
     3181 cttcttcagt caatgttccg ttttccaaat cgcgttggat atagatatcc aagaaagtgg
     3241 aagtacggcc taatgacatt gcagcaccgt tttgttgttt aacagctgcc aagtaaccga
     3301 agtataacca ttggaaagct tctttagcgt ttgtggctgg tttggaaata tcgaatccat
     3361 agatgtttcc caattctttc aattctttca aagcgcggta ttgttcgctg aattcttctc
     3421 tcaaacggat gatttcttca gtcattgtgc cgtcgccgat cttagcgaat tctttcagtt
     3481 tgtcttgcat caggtagtcc acgccataca aagcaacacg acggtaatcg ccgatgatgc
     3541 gtccgcggcc ataagcatcc ggcaagcctg tgatgacgcc ggttgttctt gctgcgcgca
     3601 tttctggagt gtacgcgtca aatacgcctt ggttatgtgt ttttctgtaa tcacggaaga
     3661 tcattgaaac ttctggatca actttgtagc cagcttcttc acaagcctgt tcgctcatac
     3721 ggataccgcc gaaaggcatc aagctgcgtt taagtggttt ttcagtctgg aaaccaacaa
     3781 tttgctcttt ttctttattc aagtaacccg ctgcatgtga tgtgatggaa gagacaactt
     3841 ttgtatccat atccaatacg ccgccagctt ctctttcttg ttttgtaaga tccattactt
     3901 gatcccataa ttgagtagtg gtttcagtag gaccagccaa gaaactttcg tcgcctgtat
     3961 attcagagaa gttgttgatg atgaagtctc ttacgtcgac ttcttctttc caagttttac
     4021 ctttaaaacc ttgccattgt tccattatat gttcctccta taaagtgccc tcacattgtg
     4081 atgtatgtaa caggtttcta cacgaacatt ataacacgta cagataaaag tgcaacaac
//
LOCUS       DAOMUJ010000119         4091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6149_length_4091_cov_1.890981, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000119 DAOMUJ010000000
VERSION     DAOMUJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_4091_cov_1.890981"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLT73_04175"
     CDS             complement(<1..243)
                     /locus_tag="PLT73_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM19610.1"
                     /translation="MAVNYPNGKKFHAESRLPKTAKKKEFIYSNRGMTLEEEINSSNE
                     HYLSSGKAVIHKKPTPIQVVKVDNPKRSTAVIKEAYY"
     gene            469..1749
                     /gene="tig"
                     /locus_tag="PLT73_04180"
     CDS             469..1749
                     /gene="tig"
                     /locus_tag="PLT73_04180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002413702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRM19611.1"
                     /translation="MTANWEKTGTNDGVLTFEISEEIIKQEMDKVFNKVKKNVSVPGF
                     RKGKVPRHVFNNIYGEESLFEDALNGALPAAYSQAIEEAGLDPVSQPKIDIKSMEKGQ
                     PWVIAAEVTVKPEVKLGEYKNLKVEKQDRVVTDEDVENNLKEKQERQAELVLKEEGAE
                     LGDTAVIDYEGFVDGVAFEGGKDQNHSLELGSKQFIPGFEEQLVGVKAGEEKEVVVTF
                     PEDYQAEDLKGKEATFKVVVHEIKTKQLPALDDEFAKDVDEEVESLDELKAKIRKELE
                     ESKNAAADEAVQDLALRQAVENAEIVELPHVMVHDEVHRQMDLFLNDMQRQGISPELY
                     YQITGTTEHDLHVQMEADADVRTKTSLVLEQIIKDENIVVSEEEIAAEIEELAKQYNM
                     DADRVRGLVSSDMLTNDIQMKKAMSIITDSAIEE"
     gene            1998..3251
                     /gene="clpX"
                     /locus_tag="PLT73_04185"
     CDS             1998..3251
                     /gene="clpX"
                     /locus_tag="PLT73_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRM19612.1"
                     /translation="MIVFHDESVGTIHCSFCGKSQDQVKKIIAGPDVYICNECVDLCK
                     EIIEEEIYAPSEAVFTEVPKPQEIQKILGEYVIGQEKAKRTLSVAVYNHYKRIGQMSM
                     MSEDDNVELQKSNICLIGPTGSGKTFLGQTLARILDVPFAIADATSLTEAGYVGEDVE
                     NILLKLLQAADFDVERAERGIIYIDEIDKIARKGENVSITRDVSGEGVQQALLKILEG
                     TVANVPPQGGRKHPQQEFIQIDTTNILFIVGGAFDGIETIVKERLGEKVIGFGSPQAK
                     HLEDKSIMQQIIPEDLLKFGLIPEFIGRLPIMAALEKLTEDDLVHILTQPKNALVKQY
                     KKLLELDGVELVFEDDALRSIAKKALERNTGARGLRSIIENIMLDIMFDIPSRDEIVK
                     CVITDNTVNGIGAPLLFDEDGKQIN"
     gene            3397..4005
                     /gene="yihA"
                     /locus_tag="PLT73_04190"
     CDS             3397..4005
                     /gene="yihA"
                     /locus_tag="PLT73_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRM19613.1"
                     /translation="MHVKEAEIVMSAVDPKQYPDTGFAEIALAGRSNVGKSSFINKLI
                     NRKALARTSSKPGKTQTLNFYLINNSFYFVDVPGYGYAKVSKTERAKWGQMLETYFTQ
                     RDTLRHVFLVVDFRHEPTEDDIQMKDFIEYYNLPYSVIATKCDKIPRGKWNQHFYRIK
                     KALNLPSDESLIMFSKETGEGFEKAWTLIEGILAEYDEAAQD"
BASE COUNT         1277 a          764 c          989 g         1061 t
ORIGIN      
        1 atagtaggct tctttgatga cggcggtact tcttttgggg ttatccactt ttacgacttg
       61 gataggcgtc ggttttttat ggatgacagc ttttcctgat gaaaggtaat gctcattgct
      121 gctgttgatt tcttcttcaa gcgtcatacc ccggttgctg tatatgaatt cttttttctt
      181 tgcagttttc ggtaaccgcg actcggcatg gaactttttg ccgttcggat aatttacggc
      241 cactggcaat cacccccatt gaatgattat accaaaaaca cacggtgaaa gaaatctaag
      301 aaagcgttaa aaatcattgg ttgaagcgtg gataacaggt gtcttttttg gacgaaaacg
      361 gaataattaa gcactttatg tttacaacat cacttttcta gtgtagtatt ggagagtgtg
      421 caatatcaaa ttaattgcaa taaaatcaat atttttggag gaaagtaaat gactgcaaac
      481 tgggaaaaaa caggcactaa cgatggtgtc ttaacatttg aaatttcaga agaaatcatc
      541 aaacaagaaa tggataaagt cttcaacaaa gtgaaaaaga acgtttctgt tcctggattc
      601 cgtaaaggta aagttcctcg tcacgttttc aacaatattt atggtgaaga gtctttattc
      661 gaagacgcat tgaacggcgc tttgccggct gcttattcgc aagctatcga agaagctgga
      721 ctggatcctg tttctcaacc aaaaattgac atcaagagca tggaaaaagg ccagccttgg
      781 gttatcgctg ctgaagttac agtgaaacct gaagtgaagc ttggagaata caaaaatctt
      841 aaagttgaaa aacaagaccg cgtcgttacc gatgaggatg ttgaaaacaa cttgaaagaa
      901 aaacaagaac gtcaagctga attggtgctt aaagaagaag gcgcagaact tggcgacaca
      961 gctgtgatcg actacgaagg attcgttgac ggtgtggctt ttgaaggcgg caaagatcaa
     1021 aaccattctt tagagttggg ttctaagcaa ttcatcccag gctttgaaga gcaattggtc
     1081 ggcgtaaaag ctggagaaga aaaagaggtt gtcgtgactt tccctgaaga ctatcaagct
     1141 gaagatctta aaggaaaaga agctactttc aaagtagttg tacacgaaat caaaacaaaa
     1201 caattaccgg ctttggacga cgaattcgca aaagatgtcg acgaagaagt agagtcattg
     1261 gacgaattga aagcaaaaat ccgcaaggaa ttggaagaaa gcaaaaatgc tgcagctgac
     1321 gaagctgttc aggacttagc tttgagacaa gcagtggaaa acgctgaaat cgttgaattg
     1381 ccgcacgtaa tggttcacga tgaagtacac cgtcaaatgg atcttttcct gaatgatatg
     1441 caacgtcaag gaatctctcc tgaattgtac taccaaatca caggtactac agaacacgac
     1501 ttgcacgttc aaatggaagc tgatgcagat gtacgcacga aaacatctct agtgttggaa
     1561 caaatcatca aagatgaaaa catcgtcgtt tctgaagaag aaatcgctgc agaaatcgaa
     1621 gagttggcga aacaatacaa catggatgca gaccgtgtcc gcggattggt cagctctgat
     1681 atgttgacta acgatatcca aatgaagaaa gcgatgagca tcatcactga ttcagcaatc
     1741 gaagaataag aaaagttgaa acagaataaa gagatcggga cattgtcttc catagtcagt
     1801 gtcccatctg tttatattca gggatatatt cgggctgtta cccccactag gtaaacgaat
     1861 catattcttt ttcaattttt cctttctgtg ttaagatgta aaccgttcga aagcggtcaa
     1921 ccggattgcc gcgctactga aaatggcgga ttctggtaag ttctgctgga tcggaaagta
     1981 agtatttagg gaggagaatg attgtgttcc atgatgaaag tgtcgggaca atacattgtt
     2041 cattctgcgg aaaatctcag gaccaagtga aaaagatcat tgcggggccg gatgtatata
     2101 tttgtaatga gtgtgtagat ctgtgcaaag agatcatcga agaagaaatc tatgcaccaa
     2161 gcgaagcggt attcaccgag gtaccaaaac cgcaggaaat ccaaaagatt ttgggcgaat
     2221 atgtgattgg ccaagaaaaa gccaagcgta cgctatctgt agcggtctat aatcactaca
     2281 aacgcatcgg ccaaatgagc atgatgtcag aagacgataa tgtagagtta caaaaaagta
     2341 atatttgttt gataggtccg actggttcgg gcaaaacatt ccttggccaa acattggcgc
     2401 gcatcctgga tgtcccattt gcgattgcag atgcgacaag tttgacggag gctggctatg
     2461 ttggtgagga tgtcgagaat atcctgttga agctgcttca ggctgccgat tttgatgtcg
     2521 agcgtgctga acggggaatc atctacatcg acgagattga taaaatcgcc cgcaaaggtg
     2581 aaaatgtatc gatcacgaga gatgtgagtg gtgaaggggt tcaacaggcg ttgcttaaaa
     2641 ttttggaagg cactgttgcg aacgttccac cgcaaggcgg cagaaaacac ccgcaacaag
     2701 agttcatcca aatcgacacg acgaacattc ttttcattgt cggtggtgcc ttcgatggca
     2761 tcgaaaccat cgtgaaagag cgtcttggcg aaaaagtcat cggtttcggc tctcctcaag
     2821 caaaacactt ggaagacaag agcatcatgc agcagatcat accggaagat ctgttgaaat
     2881 tcggattgat tccggaattc atcggccgtt taccgattat ggctgcattg gaaaagttga
     2941 cggaagatga tttggttcat attctgacac aaccaaagaa cgctctggtt aaacaataca
     3001 agaaattgct tgagttggat ggcgtcgaat tggtgttcga agatgacgca ttacggtcaa
     3061 tcgccaagaa agcacttgaa agaaatacag gtgcccgcgg attgcgctcc atcatcgaaa
     3121 acatcatgct ggacatcatg tttgatatcc caagcagaga tgaaatcgtg aaatgcgtca
     3181 taacggacaa cactgtcaat ggcatcggtg cgccgttgct gttcgatgaa gacggcaaac
     3241 agatcaactg aacatgagct tgcgtttgaa gtccggcgaa tcagacaatt tttaatctga
     3301 tttccggatt ttttcgccta gtcatggcag tcgattgcaa gaagggtttt aagcgtcggt
     3361 tttattgcag acggctttga gaaggagtga aaacaaatgc atgtaaaaga agcagaaatc
     3421 gtaatgagtg cggtcgatcc gaaacaatac ccagacaccg gttttgctga aattgctttg
     3481 gcaggaaggt ccaacgtggg taagtcttcg ttcatcaaca agttgatcaa tcgtaaagcg
     3541 ttagcgcgga catccagcaa accgggaaag acccagactt tgaacttcta tttgatcaat
     3601 aattcatttt attttgtgga tgttccgggt tatggttacg cgaaagtttc gaagacggaa
     3661 cgggcgaaat ggggccagat gctggagacg tactttacac aacgggacac attgcgtcat
     3721 gtctttttgg ttgtcgattt tcgtcatgaa ccaaccgaag acgatattca gatgaaggat
     3781 tttatcgagt attacaatct tccttattcg gtcatcgcga ccaaatgcga taaaataccg
     3841 cgcgggaaat ggaatcaaca tttttatcgc atcaagaaag cgttgaatct tccgagcgat
     3901 gagtccttga tcatgttctc aaaagaaacg ggagagggtt ttgaaaaggc ctggacgttg
     3961 attgaaggaa tattggctga gtatgatgaa gctgcgcagg actaacaaaa aaacaatggg
     4021 caacatccta ctattggatg ttgcccattg ttcgtccatt cgttatgatg ggttaaacaa
     4081 ggtgcttgtc g
//
LOCUS       DAOMUJ010000120         4088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6156_length_4088_cov_1.862881, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000120 DAOMUJ010000000
VERSION     DAOMUJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_4088_cov_1.862881"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLT73_04195"
     CDS             complement(<1..450)
                     /locus_tag="PLT73_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRM19614.1"
                     /translation="MTNSEHTKIFKAAGIEMWPAEKIQAFRKALLDWYDKEKRDLPWR
                     RTEIMLQQTRVDTVIPYYHNFLDKFPDIAALAAAPEDAILKAWEGLGYYSRVKNMQKA
                     AQQIVADYNGVFPKNPQEIAKLKGIGPYTAGAVSSIAFQIPEPAVDRN"
     gene            complement(450..1286)
                     /gene="recX"
                     /locus_tag="PLT73_04200"
     CDS             complement(450..1286)
                     /gene="recX"
                     /locus_tag="PLT73_04200"
                     /inference="COORDINATES: protein motif:HMM:NF010733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HRM19615.1"
                     /translation="MDHTEEQAEKLPVVTKITVQKKRKDRFNVFIDGEYAFPISEAVL
                     IKVGLHKGMTLTKERSAEIEKENYDYMAYTTAVNYLSYSLRSEKEVRKKLYDEEISPD
                     AIERALTRLIEQNYVNDRIYGESYTRTAANLNLKGPQLIANELKGKGLSEEDIAISLQ
                     QYPHALQLENAQTIAEKQLRKQSRVSSHEAASKIRLHLHQKGYPSDIVLEVMGEIETE
                     KEEEDEMAALRLQGDKLWRRYERKAKGRELQQKVRSGLYQKGYQGDMISAYIEEKEQE
                     AE"
     gene            complement(1433..2245)
                     /locus_tag="PLT73_04205"
     CDS             complement(1433..2245)
                     /locus_tag="PLT73_04205"
                     /inference="COORDINATES: protein motif:HMM:NF022629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HRM19616.1"
                     /translation="MDKRIEGTFANQEMLLDEITHLIKHEGYAPEQLLVITRNGKSNF
                     ITEEMAVQVIVTGEDREEDSLWDKIVKFFTVDLEEEEEEAIFERYGIDEDTYERFEDA
                     LDDGELLLLIDDAAPINDEHAEFLVRDGILPDEKAVPVAAITATKPDWASADSEEAGD
                     VPAHSIEAEKQLEVTEVAAASPTQPADVTDDITGQRIEAETVADPAMAEDSHRLPEMQ
                     FDKDDSLPELEEEKEQFSKDPFGGDTVAAETGEDAEDIEPDYEETDKPKPAL"
     gene            complement(2363..3409)
                     /locus_tag="PLT73_04210"
     CDS             complement(2363..3409)
                     /locus_tag="PLT73_04210"
                     /inference="COORDINATES: protein motif:HMM:NF018087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HRM19617.1"
                     /translation="MEKKTGKNYSNNQKRQADEQPMMEQRGKNNVGKSTAELLAQAFT
                     NGATDEKNAGPSFDIELWETILSGLRTSLQESTSAQSQKETEEPFFVLLDPVSTQPYL
                     DRAWALDRFVLDDSFLYIMSLDPQLHQTDFRTLIFFKDGRLIKTRENSNNVMFSLFEL
                     IGFDYDFIRGISTRVSGKDTHCVPYIFGRYRFLPLSGPTRKNSSWINLSKVLHSRTLK
                     GEKGVEVHFVNQHVFHLPVRPQFFTEKVKQASQTVHRQNHLLHSVLTNFDYADGIKEE
                     RKHNLLGNALHANAARLSTIPMDEYIQIVQFSLAETALRHPSLRDNPVADEALHLLRE
                     NLYGGLPHLRNAPI"
     gene            3681..>4088
                     /locus_tag="PLT73_04215"
     CDS             3681..>4088
                     /locus_tag="PLT73_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19618.1"
                     /translation="MNMKKRICTDEAATQLCDLYAGVIHHCLKKINRYPDHNDYDDFY
                     QLGLITLFDTYETCLKDALVTENSFLFVSYAKQKIHWTFLDQIRKEQKKVSREAYLCE
                     EELEEIPSTTSFEDQLEQADLLQRLCACLTAEEN"
BASE COUNT          951 a         1076 c          914 g         1147 t
ORIGIN      
        1 gttgcgatcg actgccggct caggtatctg gaaagcgatg ctggaaacgg cgcctgctgt
       61 atacggtccg atgcccttca gcttagcgat ttcctgcgga ttcttaggga aaacaccatt
      121 gtaatcggcg acgatctgtt gcgccgcttt ctgcatgttc ttgacacgag aataataacc
      181 cagcccttcc cacgccttca ggatcgcgtc ttccggcgct gccgccagcg ctgcgatgtc
      241 cgggaattta tccaggaaat tatgataata cgggatgacc gtatccaccc gtgtctgctg
      301 cagcatgatc tccgtccggc gccaaggtag atcgcgtttc tctttgtcgt accagtccaa
      361 cagcgctttg cggaaagcct gtattttctc ggccggccac atttcgatgc cggctgcttt
      421 gaatattttt gtatgttccg aatttgtcat tattctgctt cctgttcctt ttcttcgata
      481 taggcggaga tcatgtctcc ctggtagcct ttttggtaaa gacccgatct cactttttgc
      541 tgcagctcac ggcctttcgc tttgcgctca taacggcgcc aaagcttgtc cccttgcaga
      601 cgcaatgcgg ccatttcatc ctcttcctct ttctccgttt cgatctctcc cataacttcc
      661 aggacgatgt cggaaggata gcctttctga tgcagatgga gacggatttt tgaagctgct
      721 tcatgcgaag aaacccggct ttgtttgcgc agttgcttct cagcaatcgt ttgggcattt
      781 tccaactgca acgcatgcgg atattgttgc agggaaatgg cgatgtcttc ttccgacaag
      841 ccctttcctt tcagttcgtt ggcgatgagt tgcggtccct tcaggttcag gttagccgcc
      901 gtgcgcgtgt agctttcgcc atagatacgg tcattgacgt aattctgttc gatcagccgc
      961 gtcaaagcac gttcgattgc gtccgggctg atttcttcgt catacagttt tttgcggact
     1021 tctttctcac tgcgcaggct gtaggacaga taattgactg ctgtcgtata agccatgtaa
     1081 tcgtaatttt ccttctcgat ttcagcgctg cgctctttcg tgagcgtcat ccctttatgc
     1141 aagccgacct tgatcagtac ggcttccgat atcggaaagg cgtactcccc gtcaatgaag
     1201 acgttgaaac ggtccttgcg ttttttctgg acagttattt ttgttacgac aggcaatttt
     1261 tcagcctgtt cctcggtatg gtccattcta tacatccttt cggcgaatct cacttgctat
     1321 tatacacaca aaaaacgaaa ggaacaaaac gtatgttcga aatttccgcc gcgtgctaaa
     1381 aacacccagg gctgcctcat tctttcgagg cagccctggg tgtttctgtc aatcacagag
     1441 ccggtttcgg tttatccgtc tcttcataat caggctcgat atcttccgca tcttcaccgg
     1501 tttctgcagc aaccgtgtcg cctccaaatg ggtctttgga aaactgttcc ttctcttctt
     1561 ccagttccgg caaggagtca tctttgtcaa actgcatttc cggtagacgg tgactgtctt
     1621 ctgccattgc aggatccgcc accgtttcag cttcgatgcg ttgtccggtg atgtcgtcgg
     1681 tcacatcggc tggttgtgtc ggcgacgcag ctgcaacttc ggtgacttcc agctgtttct
     1741 cggcttcgat tgaatgagcc ggaacatcgc cggcttcttc gctatcggcg gatgcccaat
     1801 ccggtttcgt ggcagtgatg gctgcaacag ggacggcttt ttcgtcaggc aaaatgccat
     1861 cgcgcacgag aaattcggca tgttcatcat tgatcggagc ggcatcatcg atcagcagca
     1921 acaattcccc atcatccaaa gcatcctcga accgttcgta cgtatcctcg tcaatgccgt
     1981 agcgctcgaa aatggcttcc tcctcttcct cttccaaatc caccgtaaag aacttaacga
     2041 ttttgtccca taaggagtcc tcctcacggt cttcgccggt tacgataact tgtacagcca
     2101 tctcttccgt gatgaaattg ctttttccgt tgcgggtgat gaccagcagt tgctcaggcg
     2161 catagccctc atgcttaatc agatgcgtaa tttcatcaag caacatttcc tgattagcaa
     2221 aggtaccttc gattctcttg tccataacga ttcctcccat gactgaagat ttattctttg
     2281 aactcattgt aaaatacttc aacctgaatc acaaatgata gcctatttcc ccatcatact
     2341 aaactttact cggcaccgct gatcagatgg gtgcattcct taaatgggga agtcccccat
     2401 acagattctc ccgcagcaga tgcaacgcct cgtccgcaac cgggttgtca cgcaacgaag
     2461 gatggcgcaa agccgtttca gccaaagaaa actgaacaat ttggatatat tcatccatcg
     2521 gaatcgtcga aagccgagcc gcattggcgt gtaacgcgtt cccaagcaaa ttgtgcttgc
     2581 gctcttcttt tattccatca gcgtaatcga aatttgtcag gacgctgtgc aaaaggtgat
     2641 tttggcgatg aacggtttgg ctggcttgtt tgactttttc ggtgaaaaat tgcggacgca
     2701 ccggtaagtg gaaaacatgc tgattcacga agtggacttc cactcccttt tcccctttta
     2761 aagtccggga atgcagcacc tttgaaagat tgatccagga actgtttttg cgagtcggac
     2821 cgctcaacgg caaaaatcgg tacctcccaa aaatataggg aacacaatgg gtatccttgc
     2881 cgctgacccg tgtggagatc ccgcgaatga aatcataatc gaaaccaatc agttcaaaca
     2941 aagagaacat cacattgttg ctgttttcgc gcgttttgat gaggcgaccg tctttgaaaa
     3001 aaatgagcgt gcggaagtcg gtctggtgca gttgcgggtc caagctcata atgtacagaa
     3061 atgagtcatc cagcacaaac cgatccaaag cccaagcccg atccaaataa ggctgggtac
     3121 ttactggatc gagcaaaacg aagaaaggct cctccgtttc tttctgactc tgggccgatg
     3181 tggattcctg caaagacgtc cgcagacccg aaaggatcgt ttcccacagt tcgatgtcaa
     3241 aagaggggcc tgcatttttt tcatctgttg ctccattcgt gaatgcttgc gccaataatt
     3301 ctgctgttga ttttccgaca ttatttttac cgcgttgctc catcatcggt tgctcatccg
     3361 cttgccgttt ctgattattc gaatagtttt ttcccgtctt cttttccata gaatccccca
     3421 aattaaatgt ttaataaaaa tatatactac tttcaacccg aatgatatag aagcgcttac
     3481 attttatcgc atatccgtta aattcgtctg caaatagatg accagcaccg catttttctt
     3541 cttttttgcc gcatttgcag ttttgacctt gccgcctccg gaccgataac atagacatca
     3601 gcaagcttgc cgaggccgtg atgagcatgc acagggctct cccctgcatc acgccatcca
     3661 ctacatacgg aggcaacgcc atgaatatga aaaaaagaat ctgcaccgat gaagccgcca
     3721 cccaattgtg cgacctgtac gcgggtgtca tccatcattg tctgaaaaaa atcaaccggt
     3781 atccggacca caacgactac gacgacttct atcaattagg gttgatcacc ctttttgaca
     3841 cgtacgaaac gtgcctgaag gatgcgctcg tgaccgagaa cagctttttg ttcgtcagct
     3901 atgccaaaca aaaaatccat tggacttttc tggaccaaat ccgcaaggaa cagaaaaaag
     3961 taagccgtga agcctacctt tgcgaagaag agctggagga aatccccagc acaacttcat
     4021 ttgaagacca attggagcaa gcggatttgc tgcaacgcct ttgcgcttgt ctgacagccg
     4081 aggagaat
//
LOCUS       DAOMUJ010000121         4070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6195_length_4070_cov_2.211955, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000121 DAOMUJ010000000
VERSION     DAOMUJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6195_length_4070_cov_2.211955"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..483
                     /locus_tag="PLT73_04220"
     CDS             214..483
                     /locus_tag="PLT73_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRM19619.1"
                     /translation="MSSKDETMLFNFDDNLKKNVQETLALVYDALEEKGYNPINQIVG
                     YLLSGDPAYIPRHNDARNLIRRHERDEIMEELVKSYLSGTGRVIQ"
     gene            480..896
                     /gene="ruvX"
                     /locus_tag="PLT73_04225"
     CDS             480..896
                     /gene="ruvX"
                     /locus_tag="PLT73_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRM19620.1"
                     /translation="MRLMGLDVGSKTVGVAISDPMGWTAQGIEIVKIDEANGEFGLAR
                     IAELVKQYEVEKFVLGLPKNMDNSIGFRAEASIAYGKLLEKELGLSVDYVDERLTTVQ
                     AEKMLINEGNVSRKKRKKVIDKLAAVILLQNYLDSQ"
     gene            981..1307
                     /locus_tag="PLT73_04230"
     CDS             981..1307
                     /locus_tag="PLT73_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM19621.1"
                     /translation="MTDHNHDHDHDHNHDHDHDHEHITIVDENGNEELFEILFTFESE
                     DFGKSYVLVYPAGTPEGEEIELQAYSYVETEDGGEGNLEPIESDAEWDMIEEVLNTFM
                     EDEDLQ"
     gene            1581..2753
                     /gene="mltG"
                     /locus_tag="PLT73_04235"
     CDS             1581..2753
                     /gene="mltG"
                     /locus_tag="PLT73_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRM19622.1"
                     /translation="MSQNDQQEPQGQQKLIRPTLEKEAVDKMKIRRKERSLVRKIVFA
                     IVTIGILLGLIVGFAGYKYITDALQPLEPESTEVIEVEIPIGTSTKGITQLLEEGNVI
                     KNATIFNYYIKTQNVSDFQAGFYELSPSMSLDDIIASLQAGGSPVPQSSDHKIIVREG
                     NTIEEIAAEVEEKSDFTAEEFLAKVNDPDFLANATARYPDILTEVSQRTDTRYRLEGY
                     LYPATYDYLNGSGLDEVIMQMLQKTDDVIRPYAEQIASSGYTLHEILTIASLVEKEGV
                     TPEDRANIAGVFFNRLEIDMPIQSDISILYALNEHKELVTFKDLEIDSPYNLYKNTGM
                     GPGPFNSPSEGSIQATLSPADTEYLYFVADTETGIVYFSETYEEHLQLQNQYVDSE"
     gene            2796..3425
                     /gene="udk"
                     /locus_tag="PLT73_04240"
     CDS             2796..3425
                     /gene="udk"
                     /locus_tag="PLT73_04240"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRM19623.1"
                     /translation="MRRPIVIGVTGGSGSGKTSVSRAILDKFTEVSILLLEQDFYYKD
                     QSELPFEDRLKTNYDHPFAFDTDLFIKDLQKLIQYESIEQPVYDYSKHTRSDQVIHRE
                     PKEVIIVEGILILEDKRLRDLMDIKVYVDTDDDIRIIRRIKRDMESRGRTLDSVIHQY
                     LTVVKPMHQQFIEPTKKFADIIIPEGGQNQVAIDLMTTKIASILSEKAD"
     gene            3617..>4070
                     /gene="greA"
                     /locus_tag="PLT73_04245"
     CDS             3617..>4070
                     /gene="greA"
                     /locus_tag="PLT73_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRM19624.1"
                     /translation="MIEKAYPMTAEGKAKLEAELEDLKVNKRKEIVERIKIARSYGDL
                     SENSEYESAKDEQAFVEGRITTLEKMIRFAEIIEVQNVDTDMVSLGRKVTFVELPDGD
                     EETYIIVGSAEADPLEGKISNDSPIAKALLGKHIGEEVVIGTPGGDMNV"
BASE COUNT         1335 a          739 c          932 g         1064 t
ORIGIN      
        1 gacagatttc ctaaatttct ttcagggctg aacgaacccg ttcagctttt cttaaaacaa
       61 tctgtctgca acaaaatgcg gaatatactg acaggagggg gagcagaaag gtcatgaagc
      121 cgatctgagg aatgacaacg gcatttcttt gttgtagttt tttgtgattt cttgtaaaat
      181 ggtaaatata ctaaagaaaa ctggggtgtc gctatgagtt cgaaagatga aacgatgttg
      241 ttcaattttg atgacaatct taaaaagaat gtacaagaga cattagccct tgtttatgac
      301 gcattggaag aaaaaggata caaccctatc aaccagattg tgggatatct gctttctgga
      361 gatccagctt atatcccgcg tcataacgat gccagaaacc tgatccgtcg tcacgaacgt
      421 gatgaaataa tggaagaatt agtaaaaagc tatctttctg gaacaggcag agtcatccaa
      481 tgaggcttat gggcttggat gtcggatcca aaacggtagg agtggctatc agtgatccga
      541 tgggatggac ggcgcaaggg atagaaatcg tcaaaatcga tgaagctaat ggagagtttg
      601 gccttgctcg tattgccgaa ttggttaagc agtacgaagt cgagaagttt gtgctcggct
      661 tgccgaagaa tatggataac agcatcgggt ttcgggcgga agcttccatt gcctacggaa
      721 agttgcttga aaaagagtta ggcttatccg tcgattacgt tgatgagaga ttgactactg
      781 tccaagcaga aaaaatgttg atcaatgaag gaaacgtttc ccgaaaaaaa aggaaaaaag
      841 taattgacaa acttgctgct gtcattcttt tgcagaatta tctggacagc cagtaaagag
      901 agtggttttt ttgacctgtt tgcgttacaa tatcctttgt ttgaatgaaa aaacaatact
      961 tagataaaga ggtgcatacc atgactgacc ataatcacga tcacgatcat gaccataatc
     1021 acgaccacga ccatgaccac gagcacatca ctatcgtgga tgagaacgga aatgaagagt
     1081 tattcgaaat cttattcact ttcgaatcag aagatttcgg aaagtcctat gttctggttt
     1141 accctgcagg aactcctgaa ggggaagaaa tcgagttgca agcctattct tatgttgaga
     1201 cagaagatgg cggagaaggc aatttggaac caatcgaatc ggatgcagaa tgggatatga
     1261 tcgaagaagt cctgaacaca tttatggaag acgaagatct tcaataataa tgtaagtgac
     1321 ttgtaaggaa aacaaggaag gatcgggaca aatcatattg tctggatcct ttttttgttg
     1381 cgggatcgct tttttgaatt tttggaatcc gaatggacac gaactgaaaa tctgatgaga
     1441 aacctgtcca agatgttccg gatgtctatg caaatagcga aaaagattca atttagattg
     1501 gttttcgtgt ataattacgg aagtcgaatg gctagtagtg cataaaatag ccatatactc
     1561 aagtaaggag ggccttatct ttgtcacaaa atgatcagca agaacctcaa gggcaacaaa
     1621 aactcattcg tcctacgttg gaaaaagaag ccgtagacaa aatgaaaatt cgcagaaaag
     1681 aacggtcact agtgcggaaa attgtctttg caatcgtaac gataggaatc cttttagggc
     1741 tcatcgttgg ctttgcaggc tacaaatata taacagatgc acttcagcca ctggagccag
     1801 aaagtacgga agttatagaa gtggaaattc cgatagggac atccacaaaa gggattacac
     1861 aattgttgga agaaggcaac gtcataaaaa atgctaccat cttcaactac tacatcaaga
     1921 cgcaaaatgt ttctgatttc caggcaggct tttatgaatt atcgccttct atgagtctgg
     1981 atgacatcat tgcgtcgctt caagcaggtg gcagccctgt cccgcaatcg agcgatcata
     2041 aaataatcgt aagagagggt aatacaatcg aagaaatcgc agcggaagtc gaagagaagt
     2101 cggacttcac tgctgaggaa ttcttggcga aagtaaatga tccggatttt ttggcgaatg
     2161 cgactgcacg ataccctgat attttgacgg aagtctcgca acgcactgat acgcgttatc
     2221 gtttggaagg atacctctac ccggctactt atgattatct gaacggaagc ggtttggacg
     2281 aagtcattat gcagatgctt caaaaaacgg atgatgtgat taggccatat gcagagcaaa
     2341 tcgcttcttc aggctatacg cttcatgaaa ttctgacaat tgcctctctt gtcgaaaaag
     2401 aaggtgtcac tccggaagat cgtgcgaata ttgccggcgt attctttaat cgtttggaaa
     2461 tcgatatgcc gatacagtcg gacatcagta ttctctatgc tctgaatgag cataaggaac
     2521 tagtgacttt caaagacctt gaaatagatt cgccatacaa cctgtataaa aatacaggaa
     2581 tgggaccagg tccattcaat agtccgagcg aagggtccat tcaagccacg ttgtccccag
     2641 cggatacaga atatctgtat tttgttgcag acacagaaac cggtattgtt tatttttccg
     2701 aaacatatga agaacattta cagttgcaaa atcaatacgt agatagcgaa taagccgaat
     2761 ataaggcgtt tcaaaaagag aggaagtata caccagtgag aagacctatc gttattgggg
     2821 tgacaggggg atcaggaagc gggaaaacca gtgtcagcag ggccatctta gacaagttca
     2881 ccgaagtttc cattttgctg ttggaacagg atttttatta taaagatcaa agcgagctac
     2941 cgtttgagga tcgtttaaaa acaaactatg atcatccgtt tgcttttgat acggatttgt
     3001 tcatcaaaga tcttcaaaaa ttaatccaat acgagagcat tgaacaacca gtctacgatt
     3061 actccaaaca cacgcgaagt gatcaagtaa tccatcgcga gccgaaagaa gtcataatcg
     3121 ttgagggaat cctcatctta gaggataagc gactgcgcga tctgatggat ataaaagtct
     3181 atgtggatac ggacgatgat atccgtatta tccgcagaat caagcgcgac atggagtcaa
     3241 gaggccggac tttagactct gtcatccacc aatatctgac agtggtcaag ccaatgcatc
     3301 aacaattcat cgaaccaacc aaaaaatttg ccgatatcat cattcctgaa ggtgggcaaa
     3361 atcaagtcgc gattgatctg atgactacaa aaattgcttc aattttgtct gaaaaagcag
     3421 actaaaaata aatcacagta atattttaaa aatataattg cttttactgg aattaagtgg
     3481 tattttatat tcactattaa ttaggcagcc aatcaacgat atagagttac aggaaatgat
     3541 tatttctctt tggatgaaat gctgcctgtt ctgtatattg tgaattgaat cattacttaa
     3601 aaggggcata tttcttatga tcgaaaaagc atatccgatg acagctgaag gtaaagctaa
     3661 attagaagct gaacttgaag atttaaaagt gaataaaaga aaagaaatcg ttgaacgcat
     3721 caagattgcc agaagttatg gggatctatc cgaaaattcg gaatatgaat ccgcaaaaga
     3781 cgagcaagct tttgttgaag gccgcatcac tacgctagaa aaaatgatcc gttttgcgga
     3841 aatcatcgaa gtgcaaaacg tcgacactga tatggtttcg cttggacgca aagtgacatt
     3901 cgtggaatta ccggatggcg atgaagaaac gtacattatt gtcggtagtg cagaagcaga
     3961 tccgctggaa ggcaagattt caaacgattc tccgatcgct aaagctttgt tgggcaagca
     4021 tatcggtgaa gaggttgtca tcggtacacc aggcggcgat atgaacgtga
//
LOCUS       DAOMUJ010000122         4060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6217_length_4060_cov_2.533833, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000122 DAOMUJ010000000
VERSION     DAOMUJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6217_length_4060_cov_2.533833"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1085)
                     /locus_tag="PLT73_04250"
     CDS             complement(90..1085)
                     /locus_tag="PLT73_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRM19625.1"
                     /translation="MQLLLEQMNGYVLVAVLVLCILVLSKATDILVDEAISLSLEWGM
                     SELVIGATIVSLGTSLPELAVSISSSLKGNGILSLGNVIGSTIVNTSFILGIGALYGS
                     IPVNRKTAYKHTVLSALSLLLILSSLPIFHADGQGKIPQWLGIVFLLLTPVYFYWTVR
                     EGKKSDQEAIIEEDKEKDLSILVKLIKLLISAGFVIASASVLVSTVEIGAFRIGIPES
                     VIASTVVAFGTGLPEISTTIVSVKKGYGALAMGNIMGSNLLNTLFVLGVALGLTPGGM
                     VVSSDFYQIHFPALAILLAVLGYFSYNNKREVISRKEGRILIFFYLVYLIWNYFL"
     gene            complement(1225..1848)
                     /locus_tag="PLT73_04255"
     CDS             complement(1225..1848)
                     /locus_tag="PLT73_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19626.1"
                     /translation="MLKDRKKYWAVLVASLFLASCNQTQETAEDESSTQATAQSSSAA
                     DTTSSDKENASAESTEIGDRLGKLSKSYIEIMESGNYYMAFRSTTTFEGEMMESETMM
                     TVSGDRTAMQSKSADTETAMVMMDDNIYMIDHVSKTVIVMPQTTAEGDETLPEMPESS
                     EPVEVDDIEYIGSGEEDGLVYEEYRTEGGTQIFYYFDGSNLKKNQDD"
     gene            complement(2242..2928)
                     /gene="gpmA"
                     /locus_tag="PLT73_04260"
     CDS             complement(2242..2928)
                     /gene="gpmA"
                     /locus_tag="PLT73_04260"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRM19627.1"
                     /translation="MRKIILIRHGASEWNLLNLFTGWTDVDLSEQGVREAREAGRKLK
                     EAGIEFDMAFTSVLQRAIKTCHIVLDEMDQLWIPEVKSWRLNERHYGDLQGLNKQETA
                     EKYGEDQVLKWRRSYNTLPPLMDPDNPLSPVRDRRYANLQKRVIPMGENLETTLDRVI
                     PFWEDQIAPAVLDRKTILVAAHGNSLRALIKYIEEIPDDEIMALEIPTGQPLVYELND
                     DLTIIRKYYL"
     gene            complement(3003..>3613)
                     /gene="trpA"
                     /locus_tag="PLT73_04265"
     CDS             complement(3003..>3613)
                     /gene="trpA"
                     /locus_tag="PLT73_04265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM19628.1"
                     /translation="AIQKADERALANGINTDDIFQMVTELRTEIDTPFVFMTYINPVF
                     VYGIDKFMARCKETGVDGVIIPDVPFEEKGFIQEACRDHGITYISMVAPSSAERIAMI
                     AKEAEGFLYVVSSLGVTGVRSDITTDIGSILAEIRKISDIPCAIGFGISNAQQAKAMS
                     EVGDGVIIGSAIINIMEQYGKDSPKPVGAFVAEMVGAIRSGN"
     assembly_gap    3614..3623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3624..3791)
                     /locus_tag="PLT73_04270"
     CDS             complement(<3624..3791)
                     /locus_tag="PLT73_04270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM19629.1"
                     /translation="MNKITAAFKHKAFIPFITAGDPDLETTKTIIREIVANGADIVEI
                     GIPFSDPVAEGP"
     gene            complement(3784..>4060)
                     /locus_tag="PLT73_04275"
     CDS             complement(3784..>4060)
                     /locus_tag="PLT73_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014568938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM19630.1"
                     /translation="YPGIGPEHADLYDRGRAEYVSVTDDEAVAAFEYLARMEGIIPAI
                     ESSHAIAHAMVLAPTLDPEKIIVICLSGRGDKDVAAIARYKGVDIHE"
BASE COUNT          997 a         1087 c          885 g         1081 t
ORIGIN      
        1 ggatgttcct gataatgata acgaaagcgg tacagaagaa cgataaccaa aaaggttcgc
       61 gatccccgcg gggatcgcga accttttttc tatagaaaat agttccatat caaatacacg
      121 agatagaaaa aaatcaaaat ccgcccttct tttctgctga ttacttcccg tttattgttg
      181 taggaaaagt accccaaaac tgccaggaga atagccaaag ccggaaaatg gatctgatag
      241 aaatccgaac tgacgaccat tcctcccggt gtcaatccta atgccacacc aagaacgaac
      301 aaagtattca acagattgga ccccatgatg ttccccatcg ccaatgcgcc gtagcctttt
      361 tttacggaaa caatcgtcgt acttatttcc ggcagtccgg ttccgaaggc aacgaccgtg
      421 gaagcgatga ccgattcagg gataccaatc cggaaagctc cgatttcaac cgtactcacc
      481 aaaacagagg cactcgcaat cacaaacccc gcactgatga gcaatttaat caatttgacc
      541 agaattgaca ggtctttctc cttgtcttct tctatgatag cctcttgatc cgacttcttt
      601 ccttcccgca ctgtccagta gaaataaacc ggagtgagca aaaggaaaac tatgcccagc
      661 cactgtggta tcttgccttg tccatccgca tgaaaaatcg gcaaggaaga gagaatcaac
      721 aagagcgaaa gcgccgacaa aacagtgtgt ttatatgccg tcttccggtt gacgggtatg
      781 ctaccataaa gcgcgccgat ccccagaatg aagctggtat tcacgatcgt cgaaccaatg
      841 acattaccca aagaaagaat cccgttgccc ttcaaggacg aagaaatcga caccgctaat
      901 tccggtaggg aggttcccaa agaaacaatt gttgcgccga tgaccagttc ggacatcccc
      961 cactccaaag acaatgaaat ggcctcatca actaaaatat ccgtcgcctt tgacaacacc
     1021 aaaatacaga ggacaagcac tgcaaccagc acatatccgt tcatttgttc caatagcagc
     1081 tgcatagtcc cctcctcctt aacatgataa tctgatcttc tcatctaata agtcacttgc
     1141 tgataatccg ctggtatttc aaaagcatcc tccgaaacat tatctgacaa ttcgagaatt
     1201 tccatgatcg attcaaagga ctcatcaatc gtcttgattt tttttcaggt tggagccatc
     1261 aaaataatag aatatctggg ttccgccctc agtgcggtat tcctcgtaca ccaggccgtc
     1321 ttcctcacca ctgccgatgt actcgatgtc atccacctcg accggctcgc ttgattccgg
     1381 catttccggt agagtctcat caccctcagc cgttgtctgg ggcataacaa taaccgtttt
     1441 actgacgtgg tcgatcatat agatattatc gtccatcatc accattgcag tttcggtgtc
     1501 tgcggatttt gattgcatcg cggttctgtc gcccgaaacg gtcatcatcg tttcggattc
     1561 catcatttca ccttcaaatg ttgtagtgga acgaaaagcc atatagtaat tgccgctttc
     1621 cataatttct atgtagcttt ttgaaagttt acccaaacgg tcgccgattt cagtggattc
     1681 tgccgatgcg ttttccttgt cgctgctcgt ggtatctgcc gccgaactgc tctgcgctgt
     1741 cgcttgagtt gaactttcgt cttcagctgt ttcctgtgtt tggttgcaac ttgccaaaaa
     1801 aagactggcc accagtactg cccaatattt ttttctatct ttcagcatct taaaacaact
     1861 ccttcgaata gtgttgatcc tttcgctgca attccgattg gcgtgatgct atttccttta
     1921 tccttattat agctcatatc cagcgcgttt tgaaatttgg cataagcgtt cgagcatccg
     1981 acaaaagacc ttacacaacg cggtttttca tttcagccta acgttcggtg cgtgttattt
     2041 catccggacg gaacgctacg gcactcgctc cacgacactg atcctcatcg accattccga
     2101 tcgggtgact ttcgtggaaa gatgttacct gccagatgga accagcagtg acgtccgttt
     2161 caactttcaa ttgatggctg aagaaaaatg atgcaacaaa agatgcggcc gggattccga
     2221 ttcctggccg catctttttt gttaaagata gtatttcctg ataatcgtca ggtcatcatt
     2281 caattcatag accaacggtt gcccggtagg aatttccaag gccatgatct catcatcggg
     2341 tatttcttcg atatatttga tcaaagcccg caacgagttt ccgtgggccg ccaccaaaat
     2401 cgtcttgcga tccaataccg ccggagcgat ctggtcttcc cagaatggga tgacgcggtc
     2461 caaagtggtt tccaaatttt cccccatcgg aatgacacgc ttctgcaggt tggcgtaccg
     2521 tcgatccctg accggtgaca gcggattatc cggatccatc agcggcggca acgtattgta
     2581 ggagcgtcgc catttcagga cttggtcttc tccatatttt tcggccgttt cctgtttgtt
     2641 cagtccttgc agatcgccat agtgccgttc attgagccgc caactcttca cttccggtat
     2701 ccataattga tccatttcat ccagaacaat atggcacgtt ttgatcgccc gctgcaacac
     2761 ggacgtgaac gccatatcaa attcaatccc ggcttccttg agtttccgtc cggcttctct
     2821 cgcttcccta acgccctgtt cactcaaatc gacatccgtc cagcccgtaa acaggttcaa
     2881 taaattccac tcgcttgcgc catgacgaat caaaatgatt ttcctcatca ccatcactac
     2941 tttccgcctg tatttttctt tcattatact gctacaagcg gtctggttca cgtttaagga
     3001 cttcagttac cgctgcggat ggcccccacc atttccgcca cgaaggctcc gaccggtttg
     3061 ggcgaatcct tcccgtactg ttccatgatg ttgatgattg cggaaccgat gatgacgcca
     3121 tcaccgactt cgctcatggc tttggcttgt tgcgcgttgg aaatcccgaa gccgatggcg
     3181 caagggatgt cgctgatttt gcggatttcc gccaaaatgc tgccgatatc ggtcgtgatg
     3241 tcgctgcgca cgccggtgac tcctaaggaa gaaacgacat acaggaagcc ttcagcttct
     3301 ttggcgatca tggcgatccg ttccgcagag gaaggtgcca ccatggaaat gtaggtgatg
     3361 ccatgatccc ggcaggcttc ctggatgaag cccttctcct cgaacggaac gtccgggatg
     3421 atgacgccgt cgacgccggt ctctttgcag cgcgccatga atttatcgat gccgtagacg
     3481 aacaccggat tgatgtaggt catgaagacg aaaggcgtat cgatttcggt gcgcaattca
     3541 gtcaccattt ggaaaatatc gtcggtattg atgccgttcg ccaaagcccg ctcatcggct
     3601 ttttggatgg ccgnnnnnnn nnnaggacct tccgcaaccg gatcagagaa cgggatgccg
     3661 atttcgacga tgtccgctcc gttcgcaacg atttccctga tgattgtttt cgtggtttcc
     3721 aagtctggat cccctgcagt aatgaaaggg atgaatgctt tatgcttaaa ggctgctgtg
     3781 atcttattca tggatgtcaa ctcccttgta tcttgcgatt gctgcaacgt ccttatcgcc
     3841 acggcccgac aaacaaataa cgatgatttt ttcgggatcc agcgtcggcg ccaaaaccat
     3901 agcgtgcgcg attgcatgtg aactttcgat tgccgggatg atgccttcca tccgcgccag
     3961 gtattcaaag gcggcgacag cttcgtcatc cgtcactgaa acatactcag cgcggccccg
     4021 gtcatagagg tccgcatgct caggcccgat gcccggatag
//
LOCUS       DAOMUJ010000123         4036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6250_length_4036_cov_2.286863, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000123 DAOMUJ010000000
VERSION     DAOMUJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_4036_cov_2.286863"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLT73_04280"
     CDS             complement(<1..752)
                     /locus_tag="PLT73_04280"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRM19631.1"
                     /translation="MSTKEKVLQYLEQHKGESISGQDLADKLEVSRTSVWKAINGLKK
                     EGYQIEATTNKGYQLSIDTDLLSEAAIVPLLSEALKGHRVIAHKTIDSTNLEAKRIVN
                     EDPTFEGVILSEEQTKGRGRLGRVFYSPSESGLYMSLVLRPVADLDNATLITTAAAVA
                     VCQAIETLTGKKPQIKWVNDIFLDGRKVCGILTEGIMDMESRTIGTIILGIGLNFREP
                     ETDFPDEIQSIAGTLFDKKNAAVTRNQMVAEIL"
     gene            complement(890..1720)
                     /locus_tag="PLT73_04285"
     CDS             complement(890..1720)
                     /locus_tag="PLT73_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRM19632.1"
                     /translation="MEKVEGPFYLLNDDIIKNEDDRKHPEFEGRTVYEFIRVQDGIAI
                     FLEDHLERFFRSAAYLDLPLPATVESIEDRVYRLIAANQVEEQNLKFILGKTSEGESV
                     LWIFFTQSIYPSKKYYEEGIATSLFRIERLDPNIKLVRSDYQKSVLQERADKGVYELL
                     LVDGNEQITEGSRTNVFFVKGNELYTPPAQAVLLGIVRKKVFEICAKRQIPINETAIP
                     VEWVKEAEGAFVSGTGNNVLPISKIGDHLIPTMENPVVQAIMADYAELVRMYKEENKH
                     "
     gene            1953..2489
                     /locus_tag="PLT73_04290"
     CDS             1953..2489
                     /locus_tag="PLT73_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM19633.1"
                     /translation="MQMQQSHWKNDAEYVALVEDLIYHEELLHMETITHHHFTNRLEH
                     SIRVSYKSYQLAKKWKLDARATARAGLLHDFFHEDRDAVAQLNLGSHADAHPKIACEN
                     ACRITEISSLERDIILKHMFLVSRCGLPRYKESFVVTYVDKYVAIREVVEPIKEIAGA
                     RLRGLVSKLSPVNLNINI"
     gene            complement(2681..3475)
                     /locus_tag="PLT73_04295"
     CDS             complement(2681..3475)
                     /locus_tag="PLT73_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM19634.1"
                     /translation="MKNFKSRLFVTSATLAAIGFMATANPIQANATEYTSSTWTARTA
                     DQIKADIQNLESGSKYTFQWGDTLSAIAVATDVSVNALVDVNSIGNADMIIAGNAIVL
                     SADHKIVTVEQDSEVKSYDISSEEAVEVETPVEAVEEVQAAAPAEETYVEQTPVAQAP
                     STTYQASYFLYQVVQAEAGPSYQEKLNVASVIMNRVDSGIWGGTTIDAVLYASGQFSV
                     VANGAAAAQVPSAETIQAVNEVLNGYRSTSAQSFRASGDGVTNVFF"
BASE COUNT         1042 a          962 c          800 g         1232 t
ORIGIN      
        1 aggatttcgg cgaccatttg attccgggtc accgcggcat tttttttatc gaacaacgtg
       61 ccggcgatcg actggatctc atcgggaaaa tccgtttcag gctcccggaa gttcaagccg
      121 attcccaaaa tgatcgttcc gattgtccgc gattccatat ccatgattcc ttccgtcagg
      181 atgccgcaga ctttcctgcc atccagaaag atgtcgttca cccattttat ctgcggtttt
      241 tttcccgtca gcgtttcgat cgcttggcaa acggctacgg ctgcggctgt tgtgatcaag
      301 gtggcgttat caagatcagc aaccggcctc aaaaccaaac tcatgtacag gcctgattcg
      361 ctcggtgagt agaaaactct tcccaaacgg cctctgccct ttgtctgttc ttccgataaa
      421 atcacacctt cgaaagtcgg atcttcgttg acgatccgtt tggcctcgag gtttgtcgaa
      481 tcgattgttt tatgtgcaat gactcggtgg cctttcagcg cttccgacag taacggcaca
      541 atggccgcct cagacaacag atcggtatcg atggataact gatacccttt attcgtagtt
      601 gcttctattt gataaccttc ttttttcagg ccattgatcg ctttccacac ggatgttctg
      661 gaaacttcca atttatcagc caagtcttgg ccggaaatgc tttcgccttt atgctgctcc
      721 aaatattgta atactttctc ttttgtggac atcctttacc cccgttctct tacttagaag
      781 attaaaaaac caccgcttga accgaagcga tggtctcaat gctgtattca gtttaaacgc
      841 aggccgtctg tttgtcaacc tttttcataa tgtggttaac aatgtttttt tagtgtttat
      901 tttcttcttt atacatcctg accagttcgg cataatctgc catgattgcc tgaacaactg
      961 gattctccat cgtaggaatc aaatgatctc cgattttgga tatcggcaac acattattgc
     1021 cggtgccgga aacgaaagca ccttcagcct ccttgaccca ctccaccgga attgccgttt
     1081 cattgatcgg tatttgtcgt tttgcacaga tttcgaacac tttcttacgg acgattccca
     1141 gcaataccgc ttgtgccgga ggcgtataca gttcgttacc cttcacaaaa aagacattcg
     1201 tgcggctgcc ttcggtgatc tgctcatttc catcaaccag caaaagttca taaacccctt
     1261 tgtcggcacg ctcctgcagt accgatttct ggtaatcaga tcggacaagc ttgatgttcg
     1321 gatccaatct ttcgattcgg aagaggctcg tcgcgatgcc ctcttcataa tatttttttg
     1381 aaggataaat gctctgggtg aaaaaaatcc acaacacact ctcgccttct gacgttttac
     1441 caaggataaa cttaaggttc tgctcctcaa cctgattggc agctatcagg cgatagacgc
     1501 ggtcctcgat ggactctacc gtagcaggca gtggcaggtc cagataagct gcggaccgga
     1561 aaaaacgttc cagatgatcc tccaaaaaaa tcgcaatccc atcctggaca cggatgaatt
     1621 catatacggt ccgtccttcg aattccggat gttttctgtc gtcctcattt ttgatgatgt
     1681 catcattcaa taggtaaaaa ggtccttcca ctttctccac attcatcgcc ccttcctgat
     1741 cttatattcc ctattctact ccggaatccc aaaaagtaca cgcccattct caaaaacacg
     1801 gatgtgacag caaaatgaca aacattttaa aaattggtaa aagtgaagaa aaacacaacg
     1861 agtctcttcc tttttttcta ctgtgggtgt atagtatggg catcatgaaa aatacaaata
     1921 caactactta cataaaggat gatagaataa gcatgcagat gcaacaatcc cattggaaaa
     1981 atgatgctga atatgtcgct ttggtcgaag atctgatcta tcacgaagaa ttattgcata
     2041 tggagaccat tacccaccac cacttcacca atcgtttgga gcattcgatt cgcgtatcct
     2101 ataaaagtta ccaacttgcc aaaaaatgga agctggatgc tcgagctact gcacgtgccg
     2161 gtttgctgca tgacttcttc catgaagacc gcgatgctgt cgcccagctg aatctcggtt
     2221 cccatgccga tgctcatccg aaaattgctt gtgaaaatgc ttgccgtatc actgaaatca
     2281 gctccttgga gcgcgacatc attcttaagc atatgttttt ggtaagccgt tgtggcctgc
     2341 cgcgctacaa agaaagtttt gtcgtaactt acgtggacaa atatgtcgcg atcagagagg
     2401 tcgtcgagcc tatcaaagaa atcgcaggcg ctcgcctacg cggtcttgtt tcgaaattga
     2461 gcccagtgaa cctgaacatc aacatttaat tgtgcatttt ataaagaaac gaccgtctct
     2521 tccgatacgg tcgtttttgt atatagtttc caggatgtcc gattctttcc cggtatcgga
     2581 catcctgcaa tcctaccgga cggatccgct cgcatcaaaa aagctgaacc cgaaatgggt
     2641 ccagcttttt gctttgtact atcggtgcta tttatatttt ttagaagaac acgtttgtta
     2701 cgccatcgcc tgaagcgcgg aagctttgtg ccgatgttga acggtaacca ttcaatactt
     2761 cgttaactgc ttgaattgtt tcagcagaag gcacttgtgc tgcagcagct ccgttagcaa
     2821 ctactgagaa ctgacctgat gcatataaca cggcatcgat tgtcgtacca ccccagattc
     2881 cggaatcgac acggttcatg ataacagaag caacgttcaa tttttcttgg tagcttgggc
     2941 ctgcttcagc ttggactact tgatacaaga agtagcttgc ttggtaagtc gttgatggtg
     3001 cttgagctac tggagtttgc tcaacatatg tttcttcagc tggtgcagct gcctgcactt
     3061 cttcaactgc ttcaactggt gtttcgactt caactgcttc ttcggagctg atatcgtagc
     3121 tcttcacttc tgagtcttgt tcaacagtca cgattttatg atcagctgac aatacaatcg
     3181 cgttaccggc gataatcata tctgcatttc cgatgctgtt gacatcaacc aaggcattca
     3241 ctgatacgtc agtagctact gcaattgctg ataaagtgtc gccccattgg aaagtatatt
     3301 tgctgccgct ttccaagttt tggatatccg ctttgatttg atctgccgta cgtgctgtcc
     3361 aagtggatga agtatattct gttgcgtttg cttgtatagg gttcgctgtt gccataaaac
     3421 caatggctgc taaagttgcg ctcgttacga ataatctaga tttgaagttt ttcatgtgtg
     3481 ttttttccct ttcttctctt cgcaatgttt tcgtattgca atttatactc ttcatgacct
     3541 tttttatttg ataaatgaag tatttatcca ggtctcgatc ggagttcttt caacagagac
     3601 aatcctatca tgaatcaaac cgacaaagtt aaaaatacat tctttgttat tccgtcttaa
     3661 cgtgactgtc tcaaacaact atttatctta caaacataag tcaattatta cgctgtcgta
     3721 atatataaaa tcaatttgta acaatcagct tcattgctgt cttttttcac aaacttcata
     3781 caaaaagcaa caaaaacatt tgatttaaca acttttctat ctgtaacatt tgttttatct
     3841 ctgtcattta tcaagcgcag tcgacccatt tgacttttag tggctaaaaa tcattacaaa
     3901 acatttgctt agtgtaaaca taaataaaaa aaccttaccg acttcatcgg taaggttaag
     3961 atgcatgcat tatcagaaag cgggcgacgg gaatcggacc cgcgacatga ccttgggaag
     4021 gtcgcatttt accact
//
LOCUS       DAOMUJ010000124         4034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6254_length_4034_cov_2.611209, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000124 DAOMUJ010000000
VERSION     DAOMUJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6254_length_4034_cov_2.611209"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..>1020)
                     /locus_tag="PLT73_04300"
     CDS             complement(159..>1020)
                     /locus_tag="PLT73_04300"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HRM19635.1"
                     /translation="LGISVMLLIAILNALKLEDVIGLSIVTLIVIMVGTDDNLMLSAA
                     YRVLETFIGVIIAFLVNTFIAPPRYDERLYHTVDYATTEFLIWIRAGLRKNTEYSIMN
                     NDLKWARTQLKKMDNLYQYLTESGLFNKKNKYQNKKMLVVYRKMIQTTRSAFHVLEVL
                     HDYENVFYQFPVEMRIMIRERLETLMSGHEQIMLKFSGRVPANQVNFFEANKDQRHDL
                     MDVFFQRAQEESDFSKYSSSESYGIIHLMSAILAYEDDLVHFNKLVRSYKATPGNKSK
                     NINNIEDIIH"
     assembly_gap    1021..1030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1031..1262)
                     /locus_tag="PLT73_04305"
     CDS             complement(<1031..1262)
                     /locus_tag="PLT73_04305"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HRM19636.1"
                     /translation="MKLGARTFKTGLAVALAMIVAQYFGFEGGGVIAGIAAIYSTQPS
                     LGRSYTNLKSRIMANTIGGLVAVFVVMTLGYNI"
     gene            1544..2047
                     /locus_tag="PLT73_04310"
     CDS             1544..2047
                     /locus_tag="PLT73_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRM19637.1"
                     /translation="MHHTVVNDSIEKMKNANIRITPQRYAILEYLVESKMHPTADEIY
                     KALADRFPNMSAATVYNNLRLFVKIGFVKELAYGDASSRFDFSNTQHYHAICESCGKI
                     VDLYYPVLDDVEMVAENLTGFQVGHHRMEVYGICPECQEKGIQKEDISDGAEALPQVF
                     RGRALFA"
     gene            complement(2101..2919)
                     /gene="proC"
                     /locus_tag="PLT73_04315"
     CDS             complement(2101..2919)
                     /gene="proC"
                     /locus_tag="PLT73_04315"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRM19638.1"
                     /translation="MKKTIGFIGSGNMGGAIIGGLVGSALVAPNQVIVSSQDNESLQS
                     LKDQYAIRIADDNRQVAAEADILFLAVKPNIYNVVIDEIKTTVKEKIVIVTIAAGKSL
                     SDIEERFGRKLKVVRAMPNTPALVGEGMTGICANEVVNVAELTDVKNIFDSFGRSEMV
                     SEYMMDAVTGVSGSSPALVFMFIESLADGAVRGGMPRQQAYKFAAQAVLGSAKMVLET
                     GKHPGELKDMVCSPGGTTIEAVAELEKNGFRSAVISAVQTTIDKSIGMSKPKEN"
     assembly_gap    3115..3124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3148..>4034
                     /locus_tag="PLT73_04320"
     CDS             3148..>4034
                     /locus_tag="PLT73_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM19639.1"
                     /translation="MGNVREWWHGLIVYQVYPMSFQDSNGDGIGDIRGVINRLDYIQN
                     LGVNMIWLNPIFKSPKVDNGYDISDFLEIDPIFGTMAEVEELILEAHKRGIKVIFDFV
                     MNHTSEQHPWFQEALKGKDNPYRDYYIWADGKNGGQDLPNNWESFFTGTVWEKEPSGD
                     QYYFHLFAKEMPDLNWANPEVRRAMVDIAFFWLDKGVDGFRLDAFIHLQKEEGFPDVE
                     GLDEGEIGLAENYYANLPKINEYMKFFTSTLRERYPDTFIVGEAASATVELARSYTDP
                     VLGGCDTVITFRYFTMDEKS"
BASE COUNT         1014 a          860 c         1004 g         1136 t
ORIGIN      
        1 gagccccagt acaaggtgac cgtagggaac gttgcgtgga cagatttcct aaattgtcat
       61 gactcttgcg atttcaatcg cttagaggca tgatacactt gaccgattag gtcacagtgt
      121 ttccttaatc agggctaaac gaacccgttc agcttttctt aatggatgat gtcttcgata
      181 ttattgatgt tttttgattt attgcctgga gtcgctttgt agctgcggac cagtttgttg
      241 aagtgaacca gatcgtcttc atacgccagg atggcactca tcaggtggat gatgccgtag
      301 ctttcggaac tgctgtattt gctgaaatca ctttcttctt gggcgcgttg gaaaaagaca
      361 tccatcagat cgtgacgctg atctttgttg gcttcgaaga aattgacttg attggccgga
      421 acccgtccgc tgaatttcag cataatctgc tcatgcccac tcatcaaggt ctccaggcgt
      481 tctcggatca tgatgcgcat ttcgacagga aactgataga aaacattctc gtaatcgtgc
      541 agcacttcca gaacgtgaaa agctgatcgg gtcgtttgga tcattttccg gtagactacc
      601 agcatttttt tattctggta tttgtttttt ttgttgaaca agccgctttc agtcaaatac
      661 tggtagagat tgtccatttt tttgagttgt gtacgcgccc atttcaggtc gttgttcatg
      721 atcgagtatt cggtattctt gcgcaagccg gcacggatcc agatcagaaa ctcggtagtt
      781 gcataatcca ctgtgtggta cagccgttcg tcgtatcgcg gcggagcgat gaatgtgttt
      841 accaaaaagg caatgatgac gccgatgaat gtctccagca cgcggtaagc ggcgctcaac
      901 atcagattgt catccgtacc gaccatgatg acgatcagtg tcacgatcga cagtccgatc
      961 acatcttcga gcttgagggc attcaggatg gcaatcaaca acatcactga aataccgagc
     1021 nnnnnnnnnn agatgttgta tcccaaggtc atgacgacga aaacggctac caagccaccg
     1081 attgtattgg ccatgatccg gcttttcagg ttggtgtaag aacggccaag tgaaggttgg
     1141 gtggaataaa tggctgcgat gcctgcgatg acgcctccgc cttcgaatcc gaagtattgg
     1201 gcgacaatca ttgctaatgc gacggcgaga cccgttttaa aggtgcgggc gccaagtttc
     1261 atatagattc agctccaagt cgataaatga tgtaaaaaaa ataatcctaa ttaagaatat
     1321 actaatcccg tggccatgta aaggtcgaca ggatggttgt ttgaaaactg cctttctgga
     1381 ttcgtttcca tctgcgacat gataataaaa agtaaggatt atttttgaat gctaagagct
     1441 tatgtattgc tattttcggc aaatcgatat atgatgaatt cagctaaatg taaattataa
     1501 tgactatcat atacaagcca ttccagaaga ggggggatag acaatgcatc atacagtcgt
     1561 aaatgattca atcgaaaaaa tgaagaacgc caatattcgg attacaccac aacgttacgc
     1621 aattttggaa tatttggtcg agtccaaaat gcaccctaca gcagatgaga tctacaaagc
     1681 actggccgat cgttttccga acatgagtgc tgccactgtc tacaacaatt tgagactttt
     1741 cgtaaaaatc ggcttcgtaa aggaattggc atacggggat gcttcgagcc gttttgattt
     1801 cagcaacacg caacattatc acgcaatctg tgaaagttgc gggaaaatcg tcgatctcta
     1861 ttatccggta ttggatgatg tggagatggt tgccgaaaat ttgaccggtt tccaagtcgg
     1921 ccatcaccgc atggaagtct atggcatttg tcctgaatgt caggaaaaag ggatccaaaa
     1981 agaagatatt tctgacggag cggaagctct tcctcaggtt ttccggggaa gagctctttt
     2041 tgcatagtaa acgaaacccc taagcgagtg agcagctcag gggtttcgtt ttggttgtct
     2101 tcagttttct ttcggtttgc tcatgccgat ggatttgtcg atggttgtct ggactgccga
     2161 gatgacagcg gaccggaatc cgtttttctc caattcggca acggcttcga tcgtcgttcc
     2221 gcccggggag cagaccatat ccttcagttc gcctgggtgt ttgccggttt ccagaaccat
     2281 ttttgcggat ccgagaacag cttgtgccgc aaatttatag gcttgttggc ggggcattcc
     2341 gccgcggaca gcgccatccg ccaaagattc gatgaacatg aagaccaggg ccggggatga
     2401 accgctgacg cccgtcacgg catccatcat atattccgaa accatttcgc tgcggccgaa
     2461 gctgtcgaag atatttttga cgtccgtcaa ttcagcgacg ttcaccactt cgtttgcgca
     2521 gatgccggtc ataccttcgc caaccaaagc aggggtattc ggcatggcgc ggactacttt
     2581 taacttgcgg ccgaagcgtt cttcgatgtc gcttaagctt tttccggctg cgattgtaac
     2641 gatgacaatc ttctctttga cggtcgtttt gatttcatcg atgacgacat tgtagatatt
     2701 cggtttgacg gccaagaaga ggatatctgc ttcggctgct acctgtctgt tgtcgtccgc
     2761 gatgcggatg gcgtattggt ccttcaagga ttgcaggctt tcgttgtctt gactggatac
     2821 gatcacttgg tttggtgcaa caagtgcaga accgacgagc ccgccgatga tagctccgcc
     2881 catatttccg ctgccgatga atccgattgt ttttttcatg gatgaggact ccttttggat
     2941 gatgagtaag ttcgtttgat ttccttaatt ttatcattat aacaagaaaa aagggaaatc
     3001 gtacagaaaa tgatggaatg ctgcaaagta tgggcatcat cacatcctgt gatttgtttt
     3061 tatgaggcct tttgggtaag cttaaggtaa gtattgttcc aacgaataat gatgnnnnnn
     3121 nnnnatcacg aaagtgaggt ttttggtatg ggtaatgttc gtgagtggtg gcatggattg
     3181 attgtgtatc aagtgtatcc gatgagtttt caggactcca acggggatgg tatcggggat
     3241 attcgcggtg ttatcaaccg tttggattat attcaaaatt tgggcgtcaa tatgatttgg
     3301 ctgaatccga tcttcaaatc gccgaaagtc gataatggct atgatatttc cgacttcctc
     3361 gaaatcgatc cgattttcgg gacgatggct gaggttgaag agttgatcct cgaagcgcat
     3421 aaacgcggca tcaaagtcat tttcgatttc gtgatgaatc acacttctga acagcatccg
     3481 tggttccagg aagccttaaa ggggaaagac aatccgtacc gcgattatta tatctgggcg
     3541 gacggcaaaa acggcgggca ggatctgcct aataattggg aatctttttt caccggaact
     3601 gtctgggaaa aggaaccatc cggtgaccaa tattatttcc atctgtttgc gaaagaaatg
     3661 cctgatctga attgggccaa tccggaagtg cggcgcgcaa tggtcgatat cgcgtttttc
     3721 tggctcgata agggagtcga cggtttccgt ttggatgctt ttatccatct gcaaaaggaa
     3781 gaaggttttc cggatgtgga agggctggat gaaggggaaa tcggcttggc tgagaattat
     3841 tacgccaatc ttccaaaaat caacgagtac atgaaattct tcacctcgac tttgcgggaa
     3901 cgctatccgg acacattcat cgtcggtgag gcagcttcgg caaccgtcga gctggcgcgt
     3961 tcctataccg atcccgttct tggcggctgc gacacggtca tcactttccg ttatttcacg
     4021 atggatgaaa aatc
//
LOCUS       DAOMUJ010000125         4018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6280_length_4018_cov_3.257381, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000125 DAOMUJ010000000
VERSION     DAOMUJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6280_length_4018_cov_3.257381"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1608)
                     /locus_tag="PLT73_04325"
     CDS             complement(94..1608)
                     /locus_tag="PLT73_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HRM19640.1"
                     /translation="MTNFLDQFMGIGILVVLLVVALIIFIGRYKTASPDKALIVSGSF
                     LGSKNTHIDKATGNTMKVVRGGGTFVIPVFQTAKELSLLSSKLEVSTPAVYTEEGVPV
                     SADGTVIIKVGSTVEEIATAAEQYLSKPTEDLENEAREVLEGHLRSILGSMTVEEIYK
                     NRDKFSQEVQAVASVDLAKMGLVIVSFTVKEVTDENGYLDALGQPRIAQVKRDAHIAQ
                     AEADKETRIKRAQAEQESKEAEILRETQIAESNKQKELKLAAFRQEQDVAKAKADNAY
                     ALEKAQLDILLKEKEMNVDITERVKQIELEEKEIIRREKQYDAEVRKKADAERYAIET
                     KAAADKQKAIWESEARAKEKELNGLAESASILAIGEAEAKAKEALANALKQYGEAAIL
                     TMLIEKYPDIVRAAAEPISNIDRITVIDGGNGSQGAAKVANYASQVLTATQEGLKETT
                     GMDVTALIESFIGNKNIGSKLGALNDTLSADISADKEISPAAAGKEPESGVAVS"
     gene            complement(1609..2193)
                     /locus_tag="PLT73_04330"
     CDS             complement(1609..2193)
                     /locus_tag="PLT73_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRM19641.1"
                     /translation="MITLFRTIEPLELLFIIGVAGTVLTFPLNDILSVGFYDITDFFL
                     GILTASLLVNALQKSDSWSNASFAVAFLVVSAFIIAFKIFFVLPLLKRAENSTITSSK
                     DLEGKEATVTVPIGQKTIGEIIVSTGFGKMNRMALIYACSDTADILNIASGETVLIIE
                     VRDNIAYVTPYRNAIHSPVDKTTEWNQKKYKGGK"
     gene            complement(2407..>4018)
                     /locus_tag="PLT73_04335"
     CDS             complement(2407..>4018)
                     /locus_tag="PLT73_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 43 C-terminal
                     domain-containing protein"
                     /protein_id="HRM19642.1"
                     /translation="VNDHQAIGNKLIGNFLFTRELGEDGTGDGVGYLSAGHNSYYIDA
                     KADKEFLVFHTRFPNKGETHEVRVHQTFKNSNDWPVPTPYRYAGETITEVTQEEVTGT
                     YKFVNHGNQITGQLTESTLIQLQADGTVAGAQAGTWRLYDDYRVELKLADGTYDGVFI
                     NQWDPTTEAWVMTFSGMSESGQVIWGSQTSTTTDAQQMEKIKAELSASMPESVVKNIE
                     LPTTAAGGTTIQWTSSDPAVISETGVVTRPEADQPAAIVTLTALIQNELQSEQLSFEI
                     QVEPKEAGKLSAYYSFDETYQNAAGDQTDAQVTGDRINNTGGAVPFVEGIKGQAASFD
                     GKSGLSLGKGLITQNAYTVAFWINPAELNQFTTAFFGAATEQSWISLTPVGPANQTML
                     WSGQQWYDAPIGSTIPADTWSHVAFTVSDGKVAVYINGEERFAGEAFPNIFEGSAASF
                     GLAVNYWDQPFKGLIDELYIQDGIVLPADEIKALYDEGAAVNPDALVAETSVSQSTLL
                     YGGLAIGVMAIANVSGIYFNKSGKKTEE"
BASE COUNT          874 a         1103 c          936 g         1105 t
ORIGIN      
        1 aggtcacagt gtttccttaa tcagggctga acgaacccgt tcagcttttc ttgtgaatat
       61 tctgactgtt aggccttggc tgtatgccta cttttaagaa actgctactc ccgattccgg
      121 ttctttaccg gctgccgctg gactgatctc cttatcagct gaaatgtctg cagacaaggt
      181 gtcattgagc gcgcccagtt tgctgccgat atttttattt ccgatgaaag actcgatcaa
      241 agcagtcaca tccatcccgg tcgtttcttt gaggccttcc tgggtagcag tgaggacttg
      301 actggcatag ttggccactt ttgcagcccc ttgactgccg ttgccgccat cgattaccgt
      361 gattctgtcg atattggaga tcggttccgc agcagcacgc acgatatccg gatatttttc
      421 gatcagcatc gtcaagatgg ctgcttcccc gtattgtttc aacgcgttcg ctaaagcttc
      481 cttcgctttc gcttcggctt ccccgatcgc aaggatgctg gcagattccg ccaaaccgtt
      541 cagttccttc tctttggccc ttgcttcgga ttcccagatt gctttttgtt tgtcggcagc
      601 cgctttggtt tcgatggcat accgctctgc atcagctttc ttacgcactt ctgcgtcata
      661 ttgtttttct ctccggatga tttctttctc ttccagttcg atttgtttca cacgttccgt
      721 tatgtcaacg ttcatttctt tttctttcaa caagatgtcc agctgcgctt tttccagcgc
      781 ataagcatta tcggcttttg ctttggctac gtcctgctct tgacggaatg ccgccagctt
      841 caattccttc tgtttattcg attccgcaat ctgggtctct ctcaaaattt cggcttcctt
      901 cgattcttgc tccgcttggg cacgcttgat gcgcgtctcc ttatcggctt cagcctgagc
      961 gatatgcgca tctcgtttga cctgggcgat ccgcggttgt cccagagcat ccagataacc
     1021 attttcatcc gtcacttcct tcacggtgaa agagacgatg accaagccca tcttagccaa
     1081 atcaacactg gcgactgcct ggacttcttg cgaaaactta tctcggttct tgtagatttc
     1141 ttcgaccgtc atggaaccca gaatagagcg cagatggcct tccaaaacct cgcgggcttc
     1201 attctccaga tcctctgtcg gcttgcttaa gtactgttcg gctgctgtgg ctatttcttc
     1261 cactgtggaa ccgaccttaa tgataactgt tccgtcagct gagacaggaa ccccttcctc
     1321 cgtgtagaca gccggcgtac tgacttccaa tttgctcgac aagagcgata gctccttggc
     1381 tgtctgaaat accgggatga caaaagtacc accgccgcgc accactttca tcgtgttgcc
     1441 ggtcgctttg tcgatgtgcg tattcttgct gccaagaaaa ctcccgctga cgatcagcgc
     1501 cttatccggc gaagccgttt tgtagcgccc gatgaaaatg atcaaagcaa ccaccaataa
     1561 aaccactaaa ataccgatac ccataaactg atccaaaaaa ttcgtcattt attttccacc
     1621 tttatatttt ttttgattcc attcagtcgt tttatccaca gggctgtgga tagcatttct
     1681 gtagggcgtg acataggcga tgttatcccg gacttcaatg atcagtaccg tttctccgct
     1741 ggcgatgttc agaatatccg cggtgtcgga acaggcatag atgagcgcca ttctgttcat
     1801 tttaccgaat ccggtcgata cgatgatttc accgatcgtt ttctgcccga tcggcacggt
     1861 caccgtcgct tctttgcctt ccaaatcttt tgagctggtg atggtgctgt tttccgcgcg
     1921 cttcaataag ggcaggacaa agaaaatctt gaaggcgatg atgaaagctg aaacaaccag
     1981 aaaagccact gcgaacgagg catttgacca actatccgat ttttgcagcg cattgacgag
     2041 cagactcgcc gtcaaaatgc ccagaaaaaa atcagtgata tcgtagaagc ccacagacaa
     2101 aatatcattg agcgggaatg tcaagacagt gccggccaca ccaatgatga acagcaattc
     2161 caacggctca atcgttcgga aaagtgtaat catatttcgt catcctcctt tcgataaatt
     2221 ttttgtaaca aaaatgagcc gcattcatat tcattatcga acaaatggag tctgaagtaa
     2281 agccagaacc cttccgggct attcaaatcc ggccttgtcg gcaatcaaca aaagaggccc
     2341 cgatacagcg atccggcctc tttacatttc cgttcaagga aaacatggct tccccattcg
     2401 gatgaactac tcttctgttt tcttacctga cttgttgaag taaatccccg aaacattagc
     2461 gatggccata actccgatag caagtccgcc gtacaataaa gtcgattggc tgacggatgt
     2521 ttcggcgacg agcgcatcag gattaactgc ggccccttca tcgtacagcg ctttgatttc
     2581 atccgccggg aggacgatgc cgtcctggat gtacaattca tcgatcagac ctttgaacgg
     2641 ctgatcccag tagttgacgg ccaagccgaa tgatgcagct gaaccttcaa agatgttcgg
     2701 gaacgcctca cccgcgaacc gttcctcgcc attgatatag acggcaacct ttccatccga
     2761 aacggtaaag gccacgtggc tccacgtatc agccgggatg gtggaaccga tcggcgcatc
     2821 ataccattgc tgaccggacc agagcatcgt ctggttggct ggacctacag gcgtcaggct
     2881 gatccaactt tgctcagtgg cagccccgaa gaaggccgtc gtgaactgat tcaactcagc
     2941 tggattgatc caaaaagcta cggtataggc attctgggtg atcaagccct ttccaagcga
     3001 cagtccggat ttaccgtcga acgaagcagc ctgccctttg ataccctcaa cgaacggcac
     3061 agctccgcct gtgttgttga tccggtcccc ggtcacttgc gcatcggttt gatcgcctgc
     3121 cgcattctga taggtctcat caaaggaata ataagccgaa agcttgccgg cttccttcgg
     3181 ttcgacttgg atttcgaagc tgagttgctc cgattgcagc tcgttttgga taagtgccgt
     3241 caacgtcacg atcgctgccg gttggtccgc ttcgggacgt gtgacgacgc ctgtttccga
     3301 aatgactgca ggatcggagg aagtccattg gatagtggtt ccgcccgcag cggtagtcgg
     3361 caactcgatg ttcttcacga cggattccgg catggacgca gaaagttccg ctttgatttt
     3421 ctccatctgc tgcgcgtccg ttgtcgtact cgtttgactt ccccagatga cctgtccgct
     3481 ctcggacatg ccgctgaagg tcatgaccca cgcttctgtc gtcggatccc attggttgat
     3541 gaacaccccg tcataagtgc cgtcagccag tttcagctca acccgataat catcatacag
     3601 ccgccatgtg cctgcctgtg cccccgcaac cgtaccgtca gcctgcaact gaatcagtgt
     3661 ggattccgtc aattggccgg tgatctggtt gccgtggttg acgaatttgt aagtgccggt
     3721 gacttcttct tgggtcactt ccgttatcgt ctcgccggca tagcgatacg gtgtcggaac
     3781 cggccagtcg ttgctgttct tgaacgtctg atggacacgc acttcatgcg tctcgccttt
     3841 gttcgggaaa cgggtatgga acacgagaaa ttccttatca gctttggcat cgatgtaata
     3901 ggaattgtgt cccgccgaca gatagccgac gccgtccccg gttccgtcct cacccaattc
     3961 gcgggtgaac aggaagttcc cgatcagttt gttgccgata gcctgatggt cattgacc
//
LOCUS       DAOMUJ010000126         4014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6290_length_4014_cov_1.850972, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000126 DAOMUJ010000000
VERSION     DAOMUJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6290_length_4014_cov_1.850972"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..959)
                     /locus_tag="PLT73_04340"
     CDS             complement(66..959)
                     /locus_tag="PLT73_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM19643.1"
                     /translation="MEIRVLQYFLTIAREQSISGAAEFLHITQPTLSRQMKELEEELG
                     KQLFIRGNRKITLTDDGMLLRKRAEEIIQLVEKTESEMNADTTEFSGDIYIGGGETKG
                     MRIITRAISKAQSEHPLLKFHLFSGNAEDVTDRLDKGLLDFGVLVEPTNMSKYDFIKL
                     PTKDIWGVLMRKESPMAVLESITPKDLRDKPVICSNQLLVKNEIAGWLGGNERSLNIV
                     TSYNLLYNASLLVEENSHYALCLDGIIHTSESSDLCFRPLEPRLEVGLHFVWKKYQVF
                     SKASDYFLRLVQEEAQRQVDR"
     gene            1088..1300
                     /locus_tag="PLT73_04345"
     CDS             1088..1300
                     /locus_tag="PLT73_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19644.1"
                     /translation="MDITEEMLITNLKDAGCTKETIAAFLYYRKKNEQLKQIEILKKH
                     RHGLLDKIHEDQKAIDCLDYLLYKLK"
     gene            1326..1565
                     /locus_tag="PLT73_04350"
     CDS             1326..1565
                     /locus_tag="PLT73_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRM19645.1"
                     /translation="MIGSIAGVLTTLAFLPQVIKVLQTKDTKAISLGMYLMSVIGIFL
                     WMVHGYRIGDMALFIANVVTFCLALIILISKLKYK"
     gene            1903..2343
                     /locus_tag="PLT73_04355"
     CDS             1903..2343
                     /locus_tag="PLT73_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM19646.1"
                     /translation="MDEKGGAIMLEELAFEIAKAAHAGQTDKAGLDYILHPLQVAAEV
                     TTDEEKAVALLHDIIEDTDVTASELLAKGLPDNVVEAVEALTKKHNQNYAAYLAGVKK
                     NRLATAVKLVDLKHNSDLSRLEKITQKDRERAEKYRKAIEYLSK"
     gene            complement(2354..2728)
                     /locus_tag="PLT73_04360"
     CDS             complement(2354..2728)
                     /locus_tag="PLT73_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRM19647.1"
                     /translation="MKKYLYIGMGFLTFGLGTVGMFLPFLPTTVFYLLTGFFWVHSSD
                     RLHKQFVESEKYKQYIEEPLVKKNITNPGMVRMFVMMLFVFAIPFALTDSTLLRIVLI
                     IVYLAHLIGLTWHLRFRKSKKM"
     gene            3100..3543
                     /locus_tag="PLT73_04365"
     CDS             3100..3543
                     /locus_tag="PLT73_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM19648.1"
                     /translation="MSATDRDTLKALTVLFKSSQHVQDKLKKDMMKQGLNFSEFAVME
                     LLYHKGEQPIQNIGKKVLLASSSLTYVVDKLESKGLVQRIPCATDRRVIFTAITEEGR
                     KLMDRIFPLHAAYIADIFSVLSDKEVETFIDLAKRVGLHAENLEH"
     gene            3694..>4014
                     /locus_tag="PLT73_04370"
     CDS             3694..>4014
                     /locus_tag="PLT73_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRM19649.1"
                     /translation="MTVKNKKITKTNAMRMLDKQKCSYTVHEYAWSEDHLDALSVLEK
                     MSQDAGAVYKTIVAVGDKTGVVVGVIPAQNELDLKAFAKVSGNKRIEMLPLKNLEATT
                     GYIRG"
BASE COUNT         1164 a          854 c          914 g         1082 t
ORIGIN      
        1 ttcacctacg ctaaggatac tcgtcgccgg agctggcgaa atcaatcaga caaatcatcc
       61 gaaaatcaac ggtcgacttg tctctgagcc tcttcctgca ccaaccttaa gaaatagtcc
      121 gaggcttttg aaaacacttg atatttcttc catacaaaat gaagcccgac ctcaagccgc
      181 ggttccaacg gacggaagca caaatcgctg ctctcactgg tatggatgat gccatccaag
      241 cacagcgcat aatgactgtt ttcctccacg agcaaggagg cattatagag aaggttatag
      301 gatgtgacga tattcaggct gcgttcattg ccaccgagcc agccggcgat ttcatttttg
      361 accagaagct ggttcgaaca gatgaccggc ttatccctca gatccttcgg tgtgatgctt
      421 tccaaaacag ccattggact ctctttcctc atcaggacac cccagatatc ttttgtcggc
      481 agtttgatga aatcatattt ggacatgttc gttggctcca caagaacccc aaaatccagt
      541 aaacctttat ccaaacggtc cgtgacatcc tcggcattac cactgaacag atgaaatttg
      601 agaagcgggt gctccgattg ggctttgctg attgcgcgcg taatgatccg catgcctttg
      661 gtttcgccac cgccgatata aatgtcgcca ctgaattcag tagtatccgc attcatttcc
      721 gactcggttt tctccacaag ctggatgatt tcctcagccc gttttctcag caacatgccg
      781 tcatccgtca aagtgatctt ccggttaccc ctgatgaaaa gctgcttacc gagttcctct
      841 tccagttctt tcatttgtct ggaaagggtc ggttgcgtaa tatgcaaaaa ttctgccgct
      901 cctgaaatgc tctgttccct tgcgattgtc aaaaaatact gcaacactct tatttccatg
      961 tcttccactc cttcagtacc attttagcgc tccttgctat gcctttcaag tatgtcaagg
     1021 tatctgatat aggtatttga tgttgttgta ggttagaggt attctatttt caagaggagt
     1081 ggggtgaatg gacataactg aagaaatgct gatcacaaat ctgaaggatg ccggttgcac
     1141 gaaggaaacc atcgctgctt tcctttacta tcggaaaaag aacgagcaac taaaacagat
     1201 agagatactg aaaaaacata ggcatggctt gttggataag atccatgagg accagaaggc
     1261 aatcgattgt ctggactact tgctctacaa actaaaataa acatacaaat cagggggaaa
     1321 agaacatgat tggatcaatc gcaggcgtat taacgacatt ggcatttttg ccgcaggtga
     1381 tcaaagtttt acagacaaag gacacaaagg ccatttcgct gggcatgtat cttatgtcag
     1441 tgataggtat ctttttatgg atggtgcacg gatacaggat tggtgatatg gcgcttttca
     1501 tcgcaaatgt cgtcactttc tgtttggcct tgatcatttt gatctcaaaa ttgaagtata
     1561 aataacaaaa gaatctgttc acgctcagcg tcattaactt aagggtattt aaaaataaga
     1621 gacagtggaa acgggagata aattcactcg tatccactgt ctctttcagt tctgacaagc
     1681 aaaaaattct caagaaagca aactactcaa caaaatgggc cgaaattgcc gaataggtga
     1741 ggaacattcg gcaaattgct tctaaatcgc actgcaattg tcgaatacgc aagtaacatg
     1801 cgacaatctc ggtcccgcat ttttacagtt gtcacgttga ttaagatact gacataaact
     1861 tcacgctgat cctttttttg ttttgcagaa aagtcgatta taatggatga gaaaggtggt
     1921 gccatcatgt tggaagaatt ggcgtttgaa atagcgaaag cagcgcatgc cggacagacg
     1981 gataaagccg gactggatta tatcctgcat cctttgcaag tggctgcgga ggtgacgacg
     2041 gatgaagaga aggctgtcgc gttgctgcat gatatcatcg aggacacgga cgtgacggca
     2101 agtgagttgc tggcgaaggg tttgccggac aatgttgtag aagctgttga agcattgacc
     2161 aaaaaacata accaaaacta tgccgcatat ttggctggtg ttaaaaagaa ccggttggcc
     2221 actgccgtga agctggtgga tctgaagcac aattcggatt tgagccgtct ggagaagatc
     2281 acccaaaaag acagggagcg ggcagagaaa taccgcaagg cgatcgaata tttatcgaag
     2341 taaattgctg gtattacatt tttttcgatt tcctgaaccg caaatgccag gtcaatccga
     2401 tcaggtgggc caagtatacg attatcagta caatgcgcaa caacgtgctg tcagtcagcg
     2461 caaaagggat cgcaaagaca aagagcatca tcacgaacat ccggaccatt cctgggttcg
     2521 tgatgttttt cttcactaat ggctcctcga tgtattgctt gtatttttcc gattcgacga
     2581 attgcttatg caaacggtcc gaactgtgga cccagaagaa gccggtcagc agatagaaga
     2641 cagttgtagg caggaacggc aagaacatcc cgacagtccc gagaccgaac gtcaagaaac
     2701 ccatgccgat gtataggtat tttttcaaat ggataatctc cttcaagtct ggcaaaaacc
     2761 agattatttt tgttgcttat agttaacaat atcctcgtaa tgaagattgt ttgtgatatg
     2821 ctattcagat agtatagtac atttggctga gcgtgtgtat tagggaattc ctgattcatc
     2881 accgagaagc agctattatg aaaaatacat ctcttgtaat tttgggtgta ctgtttgccg
     2941 tttttggaat cagtgtgttt atgccttgga ttctgctccc caattatgaa acattctgct
     3001 attattccca tcatgcaagc gtttgtatat tttgacaaac tttttctttc gtgctattat
     3061 tacttcgaaa tcgaaatatt aaagggaggt ggtgttacga tgtctgcaac agatcgcgat
     3121 actttgaagg cccttactgt gttatttaaa tcatcccaac atgtacagga caaactgaag
     3181 aaagatatga tgaagcaagg tttgaatttt tccgaatttg ccgtgatgga attgttgtat
     3241 cataaaggag agcaaccgat ccaaaatatc ggaaagaaag tcttgctagc cagcagcagt
     3301 ttgacgtacg ttgtcgataa attagagagc aaagggctag tgcaacgcat cccatgtgca
     3361 actgaccgcc gtgttatttt tacagccatt accgaagaag gacgcaaatt gatggaccgc
     3421 attttcccat tgcatgctgc gtatatcgca gatatatttt ctgtcctttc agataaagaa
     3481 gtagaaacgt tcattgactt ggccaaacga gtagggctcc atgccgaaaa cttggagcac
     3541 taatcagaat ccagcgtcaa aatatcaggg actgccgcag cattgcgctg ggaagtccct
     3601 ttttttgcgc ttttttatgc aggaaccatc actttttccg gatgcaatcc ctatacatat
     3661 ggtaaaatag gcattatcag acaaaaagga aagatgacag tgaaaaataa gaaaataacc
     3721 aaaacaaatg cgatgcgcat gttggataaa cagaagtgct cctatacagt ccacgaatac
     3781 gcttggtcgg aggaccacct cgatgcgctg tccgtccttg aaaaaatgag tcaggatgcc
     3841 ggcgcggtct acaaaaccat tgtagcggtc ggcgataaga caggtgttgt cgttggggtc
     3901 atcccggcgc agaacgaact ggacctgaag gcttttgcga aagtcagcgg taataaacgg
     3961 atcgaaatgc tgcctttgaa gaatctggaa gcaaccaccg ggtatatccg aggc
//
LOCUS       DAOMUJ010000127         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6321_length_4003_cov_2.015704, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000127 DAOMUJ010000000
VERSION     DAOMUJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6321_length_4003_cov_2.015704"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLT73_04375"
     CDS             <1..260
                     /locus_tag="PLT73_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19650.1"
                     /translation="EANREELPADTTAQAAAKPNHAPAQTNKNKEIKNAERPKTQPKK
                     KGFVIKDVNDKDPQSKDKPKATTTKSAKGQKKSFQIKKIEQ"
     gene            250..1044
                     /locus_tag="PLT73_04380"
     CDS             250..1044
                     /locus_tag="PLT73_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HRM19651.1"
                     /translation="MSNDQEENIIKKYKGYLIDLDGTVYKGAERIPTAEIFVKELQDK
                     NIPFLFVTNNATKTAAEVKENLLKNFQIDVTEDHIYTSAMAALDHLSEWNIGKKVLVI
                     GEASLKQEVQSAGFEMVDEHPDFVLQALDRGVTYQQLEAACLAIQQGAKFVATNPDTN
                     LPTERGFIPGAGALTAFLKACTQKEPLIIGKPYPTIMAGAIKRMKLEKDEVIMVGDNY
                     NTDILAGVHYGVDTLMVLTGFSQRVDIEGKDEQPTYIVNDLSEWEV"
     gene            1086..>1327
                     /locus_tag="PLT73_04385"
     CDS             1086..>1327
                     /locus_tag="PLT73_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HRM19652.1"
                     /translation="MFLLVLSASIAFTINFTPLYSFDIDYLNIEQMTGLSKDIILDNY
                     RILMQYLNLPWITELKMPDFPASESGLFHFYEVKQL"
     assembly_gap    1328..1337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1338..1720
                     /locus_tag="PLT73_04390"
     CDS             <1338..1720
                     /locus_tag="PLT73_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HRM19653.1"
                     /translation="FSAETERVLAVLDYAILGVSAVVTGLFLRMVKKEQGYWRMMNPL
                     RLMIAAPLAALAIIFMNFDRLFVAFHGVFFNNDAWIFDARTDPIILALPQDFFMHCFV
                     LVFVLLELVLVLVYFWVRRKVKTT"
     gene            complement(1937..2443)
                     /locus_tag="PLT73_04395"
     CDS             complement(1937..2443)
                     /locus_tag="PLT73_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRM19654.1"
                     /translation="MVKSGSELHARAVVLMEERGVSVEDIADLVLHLQKSYVENLTMD
                     ECIENVKAVFLKREVQNAIITGIEIDKLAESGELSQPLADIILNDEGLYGIDEILALS
                     IVNVYGSIGFTNYGYIDKVKPGILQKLNDKSDSECHTFLDDIVGAIAAAAASRIAHDN
                     PNKSDIMK"
     gene            complement(2826..3757)
                     /locus_tag="PLT73_04400"
                     /pseudo
     CDS             complement(2826..3757)
                     /locus_tag="PLT73_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT         1044 a          860 c          974 g         1115 t
ORIGIN      
        1 gtgaagcaaa cagggaagaa ctgccggccg atacaactgc tcaagcggct gccaagccaa
       61 accatgctcc ggcccaaacg aacaaaaata aggaaataaa aaatgccgaa agaccgaaaa
      121 ctcaaccgaa gaaaaaagga tttgttatca aagatgtgaa cgacaaggat ccacaatcaa
      181 aggataaacc aaaggctacg acaaccaaat ctgctaaagg acagaagaaa agtttccaaa
      241 tcaaaaagat tgagcaatga tcaggaggag aatatcatca aaaagtataa aggttattta
      301 atcgatctgg acggaaccgt ctataaaggt gctgagcgta tcccgacagc ggaaattttc
      361 gtcaaagaac ttcaggataa aaatattccg ttcctgttcg tcacaaataa tgcaaccaag
      421 acagccgcgg aggtaaaaga gaaccttttg aaaaacttcc agattgatgt gacggaagac
      481 cacatctata cgagtgcaat ggcggcattg gatcacttga gtgaatggaa catcggtaaa
      541 aaggttcttg tcatcggtga agcatcattg aagcaagaag tgcagtcagc tgggtttgaa
      601 atggtggatg agcatcctga ttttgtcctg caggcgttgg atcggggggt tacctaccaa
      661 caattggagg ctgcttgttt ggcgatccaa caaggggcta agtttgttgc gacgaatcct
      721 gataccaatt tgccgacaga acgaggtttt attccaggtg caggtgcgtt gacggccttt
      781 ctgaaggctt gcacccagaa agaaccactt atcatcggaa agccctatcc aaccatcatg
      841 gcaggagcca tcaaacggat gaagttggag aaagatgaag tcataatggt cggggataac
      901 tataatacag acattctggc tggcgtccat tatggtgtcg acacattgat ggtcctgacc
      961 ggattctcgc aacgggtgga cattgaaggc aaggatgagc aaccgaccta catcgtgaat
     1021 gatctgtccg agtgggaagt gtagcgatgg ggcaaaaact gaaatatttt tttggctatg
     1081 tagccatgtt tttactggta ttgagtgcga gtattgcttt tacgataaac tttacaccgc
     1141 tttacagctt tgatattgat tacttgaaca tagagcaaat gactggatta tcaaaagaca
     1201 tcattttgga taattatcgg atcttgatgc agtatctgaa tttgccctgg atcacggagt
     1261 tgaagatgcc cgattttccg gcatccgaaa gtggattatt ccatttttat gaggttaaac
     1321 agctgttnnn nnnnnnnatt tctccgccga gaccgagcgc gtgctggcag tgctggatta
     1381 cgccatactg ggtgtgtcgg ctgttgtgac cggcttattt ttgcggatgg tgaaaaaaga
     1441 gcagggatac tggcgcatga tgaatccgtt acggctgatg atcgcagctc cattggcggc
     1501 tttggcaatt attttcatga actttgatcg tttgtttgtg gcttttcacg gcgtcttctt
     1561 caataatgat gcctggatat ttgatgcgcg gacagacccg atcatacttg cgttgccgca
     1621 agacttcttc atgcactgct ttgtgctggt cttcgtgctg cttgaactgg tgcttgtgct
     1681 agtgtacttc tgggtacgca gaaaagtgaa aacaacataa aacaaacgag cttgaactgc
     1741 tatctactat gatggcaatt caggctcgtt tttgttttgg atagtgctcg atttctcccc
     1801 gggatttgtc acactctcta aaacgtgttc tgattcgcag gggattccgc ctaagctgct
     1861 attacgtcac ggccaaaaag cggccgttct cgtaatagct tcttagtgct catcccctcc
     1921 cgcgaatcat cacactctat ttcattatgt cgcttttgtt tgggttgtcg tgggcgatgc
     1981 ggctggcggc agcggcggcg atggcgccga cgatgtcatc aaggaaagtg tggcactctg
     2041 aatcggattt gtcgttcagt ttctgcagga tgccgggttt tactttgtcg atgtatccgt
     2101 agttcgtaaa gccgatggac ccgtatacgt tgacgatgga aagggcaagg atctcatcga
     2161 tgccgtagag cccttcgtcg ttcaggataa tatccgccaa tggctgtgag agttcccccg
     2221 attcggcgag tttatctatt tcgatacccg tgatgatcgc gttctgaact tcccgtttca
     2281 ggaacacggc cttaacgttc tcgatgcatt catccatagt caagttttct acatacgact
     2341 tttggagatg caaaacgagg tctgcaatat cttcgacgct cacacctcgt tcctccatta
     2401 aaactaccgc tcgtgcatgc aactcgctgc ctgattttac catataaatt cctcctggat
     2461 tcgtatttga tacttttatt ataagggaat gacaagtgaa aacaaaagaa gaggcatttt
     2521 tgattcatta tttgcactac actgttggat ttggaagaga ggctgcctga ggaagtaaaa
     2581 aaaggtgctc tcattttatt ttgaggctgc tttatttagt tgtattcgtt cgcatactgc
     2641 gacataagct gtatggaagc accttggatt gggtcggtta tacgtgtgat gaacggaaca
     2701 tgaataaagg ccaagtattc accttttcgg atgctggggt gcgaaaaaag atggatatcg
     2761 gccaattatc catgcctaca agcaaaaaga gtatcatctc tgaaagagat agtcctccat
     2821 aaaatctaga ataaactggt actgtgcatc ggttgcacgc gggtgtccgg attgatgtag
     2881 gccatcgcat tgttgacggc tattggggct tctccgaatc cagtggcgat cagttttact
     2941 tttccggaat aggtaccgat gtctccgata gcataaacgc cagggatggt ggtttccatc
     3001 ttcgagttca cgacaactgc atttccttga acctcgaatc cccatttctt catgccgccg
     3061 atagaagacg aaaagccata attgatcaaa aagtcatcga tgtctaattc caacgtatta
     3121 tccttgcggg cttcctgaag ggttacgctc gtcaagaacg catcgtttcc gttgacactg
     3181 ataggaacga aaggtgtcat gatctcaacg gacgattctt gcagtagatg aacgctgtgt
     3241 tcttgggcgc ggaattgcgg ccgacgatgg attagataga cttttttagc gataggttcc
     3301 aaagttaagg cccaatccac tgccgaatcc ccaccgccgc agatggcaac cgtcttgtcg
     3361 cggaaacttt cgatgttgtt cacgaagtag tgcagattag tattttcgta cttgtcagca
     3421 tgttctattt ccaattttct cggccggaaa gcgccattgc cggcggataa gatcactgct
     3481 ttcgaatagt ggcgattttt ggtcgtttga atttcgaaga taccatctgc gtttttttcg
     3541 agttcaaacg cttcttcacc gaaaaccaac tttgtgtcaa agcggtccat ctgttctttc
     3601 aggttcgtta cgaggtcgcc gccggaaatg gcgggatagc ctgcgatatc gtatatattt
     3661 ttttcagcat agagcatgcc gggccgccga gctggggtag ggcttctatg attttgactt
     3721 tggcttgccg caggccggca tagaatgccg tgaacatgcc gacagggccg ccgccgataa
     3781 tggtaatgtc gtatatttct ttatctgtca attttcttcc tccgttattt atgttccggg
     3841 cgaaatgtcg cccggagcgg tatcagattt gttcgtagaa cattctgacg atgaatgtca
     3901 caaaaatacc cgtcagcagt ccgacaaaaa cttccgaagg tttgtgccca aggtattttt
     3961 ttacggccat ttcttcaggg tagattttct cctccgcttc ctc
//
LOCUS       DAOMUJ010000128         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6401_length_3965_cov_3.725831, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000128 DAOMUJ010000000
VERSION     DAOMUJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_3965_cov_3.725831"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..507)
                     /locus_tag="PLT73_04405"
     CDS             complement(172..507)
                     /locus_tag="PLT73_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19655.1"
                     /translation="MKNSKKALLIGLGATAAIAAIAAALAYEEEAVDKIGSYLNRQRM
                     KNFVKDHLKGSQKFLKAIEDLTDDEIDGFLRVVDRAGDWKESAMDLFSDFKDKAGDVK
                     DSVGDKLHR"
     gene            complement(608..3094)
                     /locus_tag="PLT73_04410"
     CDS             complement(608..3094)
                     /locus_tag="PLT73_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRM19656.1"
                     /translation="MDELFTEKASQVLLLATEEAHKFKHQSIGTEHMLLGLVREQEGI
                     AGKVLRGYDIDEEGVREEIVHLTGFGQMNAEDFNAPLPFSPRAKKVIMYATNEAHKLG
                     VPLVGTEHLLLGLLKEEILAVKIIKNLQIDPNLLRKSLYEKLGIKQPTKPDMRSAKKT
                     DKVEEGTPTLDSLARNLTDLARENRMDPIVGRDKEVRRIMQIVSRRTKNNPVLVGEPG
                     VGKTAIVEGLAQKIVAGDVPDTLANKRIMMLDMGSLVAGTKYRGEFEERMKKIIDEIY
                     NDGNVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEYQKYIEKD
                     AALERRFAPIHIDEPTPEESVEIMRGLRSRYEEHHGVEITDEALKAAVQLSVRYITSR
                     RLPDKAIDLIDESAAKVRLDVTHGDTPIGKLEMEIAKLSKDKEEAILNQDFDKAARIR
                     KREMTKKQKFQKMVEQQAQTITHYDLKVTENDVAEVVALWTGIPVNQMEQKESDRLMR
                     LEKVLHERVIGQSEAVSAVARAIRRARSGLKDPNRPIGSFLFLGPTGVGKTELAKTLA
                     EAMFGTEDSLVRLDMSEYMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKPYSVILLD
                     EVEKAHPDVFNILLQVLDDGHLTDSKGRKVDFRNTILIMTSNLGATALRDEKSVGFAA
                     KNLKHDYKAMEKRIREELKNSFRPEFLNRIDETIVFHSLEKDELHEIVKLMANSIVKR
                     LKDLDIHVRITPAAIDVIAKAGFDPEYGARPIRRAIQKEIEDKLSEEMLSGLIKFGDT
                     VTIGAQKGTIRIAVNPAKQENKDKKIPVNS"
     gene            complement(3120..3581)
                     /locus_tag="PLT73_04415"
     CDS             complement(3120..3581)
                     /locus_tag="PLT73_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRM19657.1"
                     /translation="MHGQNMSDIIEAYIKKILNAEEQIEIRRNEMADRFNCVPSQINY
                     VINTRFTEQQGYAVESKRGGGGYIRIMKVKILDQAELLDKLIAIVGESITQKDAFAVT
                     QNLYSRGIITKREGNLMLSVLDKSLAPYTGEHEERIRALLLRNFLNSLRFE"
BASE COUNT          925 a         1017 c          818 g         1205 t
ORIGIN      
        1 atagtatgga gaatgttctt attcatgcct ctattttacc actgtggaac caaaaaaaaa
       61 actgtgaaac atgcgccagc acaacattcc acagccgaaa aattattgtt tcctactata
      121 caaaaaaaac aaggattgtc tgcagatgag acaatccttg tttcaacatt cttatctatg
      181 caacttatcg cctacgctgt cttttacatc gccggccttg tccttaaaat cagagaatag
      241 gtccattgct gactctttcc aatcgcctgc tctgtcgaca acgcgcaaga atccatcgat
      301 ttcatcatcc gtcaaatcct cgattgcctt caaaaacttt tggctgccct tgagatggtc
      361 cttcacaaaa tttttcatgc gctgacggtt caagtaggag ccgattttat ccactgcttc
      421 ttcctcgtat gccaaagctg ctgcaattgc cgcaatcgct gctgtagcac ccaaaccgat
      481 caacaatgct tttttagaat tcttcatctt gtttgttccc tcctctaact tctgtacctg
      541 tattttagca caaacggtag cgatagccga acaataaccc tgcctgccaa atgtggacac
      601 gccactttta ggaatttacg ggaatttttt tgtctttgtt ttcctgctta gcgggattca
      661 cggcaatccg gatcgtgcct ttttgggcgc cgatcgtaac ggtgtcgccg aatttgatca
      721 agccgctcag catttcttca ctcaacttat cttcgatctc tttttggatc gcgcgtctga
      781 tcgggcgtgc gccatattcc ggatcgaatc cagcttttgc gatgacatcg atggctgcag
      841 gcgtgatgcg cacatggata tcgagatcct tcagacgttt cacgatactg ttcgccatca
      901 atttaacgat ttcatgcaat tcgtccttct ccaatgagtg gaagacgatc gtttcgtcga
      961 tacggttcag gaactccggg cggaaactgt tcttcagttc ttcacggata cgtttctcca
     1021 tcgccttata gtcatgcttg aggttcttcg ccgcgaatcc gactgacttc tcatcacgta
     1081 aagctgttgc ccctaagttt gaggtcatga tcaagatcgt attgcggaaa tcgactttcc
     1141 gaccttttga atcagtcaga tgaccgtcat cgagcacttg caggaggata ttgaagacgt
     1201 ccgggtgggc tttttccacc tcatcaagaa gaatgaccga gtacggtttt tgacggattt
     1261 tctctgtcaa ttgaccgcct tcatcatagc cgacatagcc gggtggagag ccgatcagac
     1321 gacttgtgct gtatttttcc atgtattcgg acatatccaa acgaaccaag gagtcttctg
     1381 tcccgaacat tgcttccgcc aacgttttcg caagctctgt tttgccgact ccagtagggc
     1441 ccaggaacag gaaggaaccg attggacggt ttggatcctt caaaccggaa cgtgcgcgtc
     1501 tgatggcgcg agccaccgca ctgaccgcct cggattgccc aatgacgcgt tcatgcaaca
     1561 ctttttccag cctcatcaaa cgatcgcttt ccttctgctc catctggttg acaggaatcc
     1621 cggtccacaa agccacgact tcagctacat cattttcggt tactttcagg tcgtagtgcg
     1681 tgattgtttg ggcttgttgt tccaccattt tttggaattt ctgcttcttc gtcatttcac
     1741 gtttgcggat gcgggcagct ttatcaaaat cctggttcag gatcgcttct tctttgtctt
     1801 tcgacaactt cgcaatttcc atctccagtt ttccgatcgg tgtatccccg tgtgtgacat
     1861 ccaaacggac tttcgcggcc gattcatcaa tcagatcaat cgctttatcc ggcaatcgtc
     1921 tggaagtgat gtagcgcacc gatagctgga ccgctgcttt caaggcctca tccgtgattt
     1981 ccacgccatg gtgctcctca taacgggaac gcaagccacg catgatttca accgattcct
     2041 caggtgtagg ctcatcgata tggataggtg cgaaacgtct ttccagcgcc gcatcctttt
     2101 cgatatattt ttggtactca tctagggtgg tggcaccgat cgtctgcaat tcgccccttg
     2161 ccaatgccgg tttcaggatg ttggaagcat cgattgcccc ttccgctccg cctgcaccga
     2221 tcaaggtgtg caactcatcg atgaagagga tgacattgcc gtcattgtag atttcatcaa
     2281 tgattttttt cattctttcc tcgaattcac cgcggtattt cgtacccgct accagcgaac
     2341 ccatatccaa catcatgatg cgcttgtttg ccaatgtatc cggaacatcg ccggctacga
     2401 ttttctgtgc caaaccttcc acgatggcgg tcttgccgac tcccggttcc cctaccaaaa
     2461 caggattgtt cttcgttctg cgggaaacga tttgcatgat acgacggact tccttgtcgc
     2521 gtccaacgat cggatccatc cggttttcac gcgccaagtc agtcagattg cgggccaacg
     2581 aatccaacgt cggcgttcct tcttccactt tatccgtctt tttggctgaa cgcatatccg
     2641 gtttggttgg ttgtttgatg cccagtttct catagagaga cttacgcaac aaattcggat
     2701 cgatctgcag atttttgatg attttgactg caagaatttc ttctttcaat aagcccaaca
     2761 aaaggtgttc ggttcctacc aagggaactc ctaatttgtg tgcttcgttt gtcgcataca
     2821 taatgacttt ttttgcccga ggagaaaatg gcaacggtgc attgaagtct tccgcattca
     2881 tttgaccgaa accggtcaga tgaacaatct cttcgcggac tccttcctca tcaatatcgt
     2941 agccacgcaa cacttttccg gcaatgcctt cttgttcccg gactaatccg agaagcatat
     3001 gttccgttcc gatggattga tgcttaaatt tgtgcgcttc ttctgttgcc aacaacagta
     3061 cttggctggc tttttctgta aataattcat ccatttttaa cgcctcctct acgggctatc
     3121 tattcaaagc gcagactgtt caagaaattc ctcaacagca aggctcgtat tctttcttca
     3181 tgttcgcctg tgtatggggc aagtgactta tccaataccg acaacatcag gtttccttca
     3241 cgtttcgtga tgatcccgcg actgtataga ttttgggtta ccgcaaacgc atctttctgg
     3301 gtgatgcttt cgcctactat agcgataagt ttatccaaca attctgcttg atcgaggatc
     3361 ttcactttca tgatgcgtat ataaccgccc ccgccgcgtt tgctttcgac cgcgtagcct
     3421 tgctgttccg taaaacgggt attgatgaca tagttgattt gtgaaggtac gcaattgaaa
     3481 cgatccgcca tttcattcct tctgatttca atctgctctt ctgcattcag aatctttttg
     3541 atgtatgctt cgatgatatc tgacatattc tgaccatgca tggaatggac gcctcccttc
     3601 ccctgactaa ttttgactat tatatattta tattatgcta ttcctacata actgtcaaat
     3661 ggaaagagct atccgagggc ttttacagca aattattcaa gagagctttg aagctcatcc
     3721 gtactgctct gcagaccctt tataacagca tatttcacta acatcttcga caagattacc
     3781 gaagaatgtt actcattata tactgaaagg tgaaaaacgg caatatttct atctgaaaag
     3841 ctgacagggg ttggttaata caaaaataga cgcagaaatc atcggaatga tttctgcgtc
     3901 tattttattt atcgggaaga caggattcga acctgcgacc ccttggtccc aaaccaagtg
     3961 ctcta
//
LOCUS       DAOMUJ010000129         3934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6472_length_3934_cov_2.338747, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000129 DAOMUJ010000000
VERSION     DAOMUJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_3934_cov_2.338747"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLT73_04420"
     CDS             <1..487
                     /locus_tag="PLT73_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19658.1"
                     /translation="YANLQVFKGYAEKEYLPYTTSAGDVAALDAALDNILEEGIETVF
                     ARHDRIASAVRGSAQAYGLELFLDSDRSNTVTAIRIPEEIGALRLQDHLSENYNLLVA
                     TSLAQYADVILRIGHMGENAFIDKLIYVLSIIDNGLRDLGFAGNGDLAQLFIDIYKEN
                     N"
     gene            499..1035
                     /locus_tag="PLT73_04425"
     CDS             499..1035
                     /locus_tag="PLT73_04425"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM19659.1"
                     /translation="MQKAQGYSNTRTYDLVLTAILIALVFVATVFLNIKLPITANGGL
                     IHLGTAMLFLASILFGPKKGAIAGAVGMAIFDLMAGWVLWAPFTLVTRGLQGYLVGKL
                     AWSKDRQGNSFAVNLLATLLSVPIMLVGYYLCEWILYGNALTPFASIPGNLVQNAVGI
                     AISLPLSAILKKMNIAGR"
     gene            complement(1485..1616)
                     /locus_tag="PLT73_04430"
     CDS             complement(1485..1616)
                     /locus_tag="PLT73_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19660.1"
                     /translation="MKNIVYTNSQNVTIEISWEAYYKEIDMRKQERLKKKQAQTSGK"
     gene            complement(2022..2771)
                     /gene="nfsA"
                     /locus_tag="PLT73_04435"
     CDS             complement(2022..2771)
                     /gene="nfsA"
                     /locus_tag="PLT73_04435"
                     /inference="COORDINATES: protein motif:HMM:NF008033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HRM19661.1"
                     /translation="MNEMIARILAHRTVRDFEERPLSPEQIETIVRSAQAASTSSFVQ
                     AYSIIGVTDRDKKHALSKIARNQHFVAENGHFFVFCADLHRHDVIGKMEGKDVADSLE
                     STEKFMVSVVDASLAAQNAALAAEAMGLGICYVGGLRNDLEKVSELLKIPDKVIPLFG
                     LAVGYPASVNDQKPRLPFEHVYHENEYQQNNAVYKEQLKQYNEEIQAYYRDRTEGERC
                     DTWTGQMAEMLSHPQRMYMHDFVEKQGMNKH"
     gene            complement(2932..3213)
                     /locus_tag="PLT73_04440"
     CDS             complement(2932..3213)
                     /locus_tag="PLT73_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HRM19662.1"
                     /translation="MKVKKKISLTPFSKVKSLILSLFDSKVEGKKKWLVLGIILYIIS
                     PIDIIPDFIPMAGYADDVLLPILFLVAEKLLSDGAIEENERPIKEAKKI"
     gene            complement(3365..>3934)
                     /locus_tag="PLT73_04445"
     CDS             complement(3365..>3934)
                     /locus_tag="PLT73_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM19663.1"
                     /translation="EETMEALHDVVKSGKARYIGASAMYAWQFQKALHVAEKNGWTRF
                     VSMQNHLNLLYREEEREMLPLCREEKIAVTPYSPMASGRLTRDWSETSKRMETDNVAR
                     SKYDATADKDRVIVERLAQIAADRGVSRVEIALAWLLQKEPVVAPIIGATKLEHLEKP
                     VEAISVKLTDAEVAFLEEPYVPHLIVGHS"
BASE COUNT          927 a          991 c          898 g         1118 t
ORIGIN      
        1 ttatgccaat ctacaggtct tcaaaggcta cgcggaaaaa gaatacttgc cctacacgac
       61 ttctgctggc gatgtcgcag cgcttgatgc cgctttggac aacatcctgg aggaaggcat
      121 cgagaccgtt tttgcacgcc atgacaggat tgcttccgca gtacgcggat ccgcccaagc
      181 gtatggattg gagttgttcc tggattccga ccgttccaat acagtcactg ccatcaggat
      241 accggaagaa atcggcgcct tgcgcctgca ggaccacctc agcgaaaact acaatctgtt
      301 ggtcgcaact tcattggcac aatacgcaga tgtgatcttg cgcatcggac atatgggcga
      361 aaatgccttc atcgacaaac ttatctatgt tttgtccatc atcgacaacg gtctgcgcga
      421 tctcggcttc gccggaaacg gggatttggc ccaacttttc atcgacattt acaaagaaaa
      481 taattaggag gcttcactat gcaaaaagca caaggttatt caaacactcg cacgtatgat
      541 ttggttttga cggcaatcct gattgcgctc gtattcgtag cgacggtctt cctgaacatc
      601 aaattgccta tcacggcaaa cggcggactg atccacctcg gcactgcgat gctcttcctc
      661 gcatccatcc tgttcgggcc gaaaaaaggc gctatcgccg gagcggtcgg catggccatc
      721 ttcgacctta tggccggatg ggttctgtgg gctcccttca cattagtaac ccgcggcttg
      781 cagggctatc tggtcggtaa attggcctgg tcgaaagatc gtcaagggaa cagctttgct
      841 gtgaatcttt tagccacgct gctttcggtt cccatcatgc tggtcggtta ttatctgtgc
      901 gaatggatcc tgtacggcaa tgcccttacc ccgttcgctt ctattcccgg taacctcgtc
      961 cagaacgcgg tcggaatcgc catttccttg ccgctgtcgg caattttgaa aaagatgaat
     1021 atcgctggaa gataacgaat tgaaaaggaa ggctctgtct cgggatgaga cagagccttc
     1081 ctttttggta atagtcctcg ggttgtctga ttctttaacg gtatcggatt ggtatagagt
     1141 gggtgaagcg tgcaatttta tttggttatt gcagatagca ccggccagat gcccgtattg
     1201 acgaataaag tacctgtatt cgtcaatctt gcctttcgct atgcccaaat tgtcgaatgt
     1261 ttttgaacat tcgacaaatc agctctctat tggggtcaaa ttgttgaata attttgtagc
     1321 attcgacaat ttgattcgcc gaacgaaact ttgggatgca ggaccacccg ctccttgaaa
     1381 cggaaacaaa aaaaaagccc ggaagccgct gcctcaacaa ctgtgagtca gggattccga
     1441 gctttttatt tacagttctc cgttcataga gagccgctta gaccttattt gccgcttgtt
     1501 tgcgcttgtt ttttcttcaa acgctcttgt ttgcgcatgt cgatctcttt gtaatacgct
     1561 tcccatgata tttcaatagt cacgttctgc gaattcgtat agacgatgtt cttcatatat
     1621 ttcttgctcc ttctttagcg taaccatatg cgtacgctac tatctttcaa cctcctcata
     1681 tatccttttt ttctgtattc agagaaatca aaaaggcact caatcgagta ccttttgtgt
     1741 ctctacttat tatcataaga aagaaaaagg tattcctgta caggaatcgc ggctttttca
     1801 ctatcaaatc taaatcttgg aaattcatgt tgcgggcacc acagtgtggc gctacataat
     1861 aaatttaact tcgcttattt ttggttccgt ttgcctgcag catccgctgc agaatagaga
     1921 aaagccatgg ttccatacat ataagatgca tatgttcttg gacgttgaaa ggtaactata
     1981 acacgaatat ccgttcgatg caagtgaaac tatttctgga atcagtgctt gttcattcct
     2041 tgcttttcga cgaagtcatg catgtacatg cgttgcggat gagagagcat ctctgccatc
     2101 tggccggtcc aagtgtcgca gcgttcgcct tcggtccgat cccgatagta cgcttggatc
     2161 tcttcgttgt actgcttcag ttgttcttta taaaccgcat tattctgttg atactcattt
     2221 tcatgataaa catgttcgaa aggcaggcgc ggtttctgat cattgacgct ggccggataa
     2281 ccgaccgcca gtccgaagag cgggatcact ttatcgggaa tcttcaggag ttcgctgact
     2341 ttttcgaggt cattccgcaa gccgcctacg taacagatcc cgagccccat cgcttcagcc
     2401 gccaaagctg cattctgggc agccagggat gcatctacca cgctgaccat gaatttctcg
     2461 gtgctctcaa gcgagtctgc aacatccttc ccttccatct ttccgatcac atcgtggcga
     2521 tgaagatcgg cgcaaaatac aaagaaatgg ccattctcgg caacaaaatg ttggttacgc
     2581 gcgattttcg acaatgcgtg ttttttgtcg cgatccgtga ctccgatgat ggaataggcc
     2641 tgcacaaaac ttgaggtgga tgcagcttgg gcactacgga cgatcgtttc tatctgttcc
     2701 gggctcaaag gccgttcttc aaaatccctt accgtgcgat gcgccaggat gcgcgcgatc
     2761 atttcattca tctattgttt tcctcgcctt cagaatagct tatattgaat ccataagatc
     2821 attctaaagg aatgcagcag ccacgtccaa ccgggtgctc aaggtcccaa cagcgaaaaa
     2881 gccccgctcc tttgccattt aggcaaggaa cggggccgga aaatccaggc attatatttt
     2941 tttggcttct ttgattgggc gttcgttttc ttcaatcgct ccgtcgctca ataatttttc
     3001 agcgaccaaa aacaggatcg gcaacagcac atcatccgca taaccggcca tcgggataaa
     3061 atccgggata atgtcgatcg gactgatgat ataaagaatg atccccaaca ccagccattt
     3121 tttcttgcct tcgactttgg aatcgaacag ggaaaggata agtgacttga ctttactgaa
     3181 aggtgtcaga ctgattttct tttttacttt cattttgcat tttcctttgc tgattgattt
     3241 ggacaaccat tcttcgcacc ttgcttgtga gcgagctctg actagttatc gtgaacaaag
     3301 tatactgctt atcttaagta aatgaatcag tctcaagaat gattttttgg cctgtctgaa
     3361 tgcctcagct gtgcccgaca atcaaatgtg gaacataagg ctcttccagg aacgcgactt
     3421 cggcatcggt cagtttgacc gatatggctt ccaccggttt ttccagatgc tccagcttgg
     3481 tcgctccgat gatgggagct acaaccggtt ccttctgcag cagccaagcc aaggcgattt
     3541 cgacacggga aacgcctctg tcggcggcta tctgggccaa tcgttcgacg atgacccggt
     3601 ccttatcggc agtggcgtcg tatttcgaac gcgccacatt gtccgtttcc atccgtttgg
     3661 acgtctccga ccagtcgcgc gtcaaacgac ctgaagccat cggactgtag ggggtcacgg
     3721 cgattttttc ttcccggcaa agcggcagca tttcccgctc ctcttcacgg taaagcaggt
     3781 tcagatggtt ctgcatcgat acgaagcgcg tccaaccgtt cttttctgct acgtgcaggg
     3841 ccttttggaa ctgccaggca tacatcgcgg aagctcctat gtaacgggcc tttccggatt
     3901 tcaccacatc gtgcagagct tccatcgttt cctc
//
LOCUS       DAOMUJ010000130         3927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6491_length_3927_cov_2.294938, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000130 DAOMUJ010000000
VERSION     DAOMUJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_3927_cov_2.294938"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..510)
                     /locus_tag="PLT73_04450"
     CDS             complement(40..510)
                     /locus_tag="PLT73_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM19664.1"
                     /translation="MFQEYKKILIPVDGSRESELSFRKAVEVAKRNKAAIIITHIIDT
                     RAIQTPTGFEGSFTDEIVRQSKTLMEEYKIYATNEGITDVQTVIEYGSPKAIIAKDLP
                     AEYGVDLIMIGATGLNAIERLFIGSVSEYVIRNAPCDVLVVRTDLENKPKTKKS"
     gene            complement(609..911)
                     /locus_tag="PLT73_04455"
     CDS             complement(609..911)
                     /locus_tag="PLT73_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19665.1"
                     /translation="MLELLIALFSLLLVSTGLFMTLKGPTVFVLAPDRITEQQRQQIW
                     LFFRNGGALYMLIGFLCLLTIFFRSTLLYVFAVLISSGYTALFSIRLAGMMKDQSH"
     gene            complement(1026..1215)
                     /gene="ssrS"
                     /locus_tag="PLT73_04460"
     ncRNA           complement(1026..1215)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLT73_04460"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1320..2583)
                     /locus_tag="PLT73_04465"
                     /pseudo
     CDS             complement(1320..2583)
                     /locus_tag="PLT73_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
     gene            2773..>3927
                     /gene="gshAB"
                     /locus_tag="PLT73_04470"
     CDS             2773..>3927
                     /gene="gshAB"
                     /locus_tag="PLT73_04470"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF002688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HRM19666.1"
                     /translation="MTDFKEIILTHKLSALFSKASFGIEKESQRITDEGTIAKTNHPT
                     IFGNRSFHPYIQTDFAESQPELITPPMQSIEEMHEWLMAIHDVVLRSLPEGEYLLPCS
                     IPPAMPASEEIKVAKLDNESDVAYREYLVSVYGNKKQMVSGIHFNFELNPVLIKELHQ
                     LSGSVRTLREFQSDVYLKMAHNFIRYQWIMTYLLGGSISADRSYFEKESQHDLPLDQY
                     TRSIRSSKYGYVNKADVHVSFESIDAYVQDIEKMVTTGKLIAEKEFYSTVRFRGANKA
                     RDLLTNGIAYLEFRLFDLNPFAEFGMHKEDMYFIHYFLLYLLWIDQDASEAEMQLGKE
                     MNYSTALENPLQPSAFQAEGLSVLEGMLQMLEAIDAEEKISAIVKEKIEAF"
BASE COUNT         1099 a          876 c          911 g         1041 t
ORIGIN      
        1 ggtcacagtg tttccttaat cagggctgaa cgaacccgtt cagcttttct tagttttggg
       61 tttgttctcg aggtcagtcc ggactaccaa cacatcgcaa ggtgcgttgc ggatgacata
      121 ctcggatact gagcctatga aaagtctctc gatggcatta agaccagtcg ctccaatcat
      181 aattagatcc acgccgtatt cggcaggcaa atcttttgcg atgatcgctt ttggagaacc
      241 atattcgatg actgtttgaa catctgtaat gccttcattc gtagcataga ttttatattc
      301 ttccatcaac gttttggatt gacggacaat ctcatctgta aagctaccct cgaaacctgt
      361 aggggtctgg atggctcttg tgtcgataat atgagtgatg atgatggctg ctttattccg
      421 tttggctact tccactgcct tacggaaaga taattctgat tctctggatc cgtcaaccgg
      481 tatcaaaatc ttcttatact cttgaaacat attaaccact cctctaaact agtatcgctt
      541 acaagtttat tttacttcac tttccttaaa aatgaaagcg catgcgattt ttttcggtaa
      601 tttattgatc agtggctttg atctttcatc atgccggcca agcggatgct gaagagtgct
      661 gtataccctg aggagatcag cacggcaaat acataaagca gggtactcct gaaaaaaatc
      721 gtcaggaggc agagaaatcc gatcaacatg tacagagctc cgccattacg gaaaaagagc
      781 cagatctgct ggcgttgttg ttccgtgatc cggtccggag ccaatacgaa aacagtgggg
      841 cctttcaacg tcatgaaaag gccggtcgaa accagcagta atgaaaaaag ggcaatcaac
      901 aattctaaca atatgcatcc tccttaaagg aattgtggga taagagctca agtagtactt
      961 ttacgtgctt ttaaatctgc aaaaaaagct gctcaatgag cagctccaaa agtataaaac
     1021 tttattagaa atccgatggt gccggtgagt atattctact agttttgaac ccgcatataa
     1081 gcaggtgggc tccaatatcc agtctctaac taccaaatga taaggcgtgt tatccaccga
     1141 aataaacagg atcccaaggt agtaataaat tgttcggtca acacattgag aatatgtcga
     1201 acacctcaga aatctatata cgtatagtag catactaatc aattgttggc aagcaaaaca
     1261 catggaactg agtccggaaa cataatggcg acaatgggtt aaacgctacc aacaaatcat
     1321 caagaatctt tgttgaagcg gtgctgcaat tggttggcca aggcttgctc atatttgccc
     1381 gttgcagccg gctcgtagta gcgtctactc ttcagcgagt ctggtaaata ctgttgtttg
     1441 acccagtggt ccgggaaatc gtgcggatat tgataaccga tgccgcggcc taacttattt
     1501 gccccgctat aatgggcatc acgcaaacta tccggtatgt cgccggactt tccggcgcgc
     1561 acatccgcca gtgcgctatc caaggcaaga taagccgaat tggatttcgg ggaaagggcc
     1621 agatcgacga cagcatttgc caggggaatc cgagcttccg gcaaacctaa cttttctgca
     1681 gcctgaacgg ccaaaacggt ccgagccaca gcttgaggat tgcctaggcc gatatcttcg
     1741 taggcgcaga ccatcagtcg tctgcaggcg atcagcaatt cgccggcttc cagcagcctg
     1801 gcaagataat gcatggctgc atcgacatca ctgccacgga tcgatttttg gaatgccgag
     1861 atgacatcat aatgggcatc gccattctta tcgtgaacca gggctttctt ttggatgcac
     1921 tcctcagcga tgtccaaggt gatatggatc gttccgtccg catcaggttt agtggataag
     1981 gcagccaact ccaaagcatt cagcgaaccg cggacgtctc ccatcgtact cctggcgaaa
     2041 tggagcaagg cgtcttcctc gatcaggatc ttttcttttc ccaatccgcg cttttcatcc
     2101 gcaatggcac gttccagtgc ctgtatgacg tcttgagtag taaggggctt caattcaaaa
     2161 atttgggtcc ggctccggat agcaggactg atgctgatat atgggttttc ggtggtggcg
     2221 ccgatcagaa tgacctggcc gctttcaagg tgcggcaaga gaaaatcctg cttgggttta
     2281 tccaaccgat gcacctcatc caacaagagg atgacctggc cggaaaattt ggcttcttcc
     2341 acgacgatct gcaagtcttt ttttgtgtcg gttgcggcgt tcaactgacg gaaagccgaa
     2401 tgggtcgatc cggcgatggc gctcgcaatg ctggttttac cgattcccgg tgggccatac
     2461 aatatcatgg aagaaagcat cgaccatgcg acggatgatt ttattttcgc cgaccagatg
     2521 actttgaccg atgacttcgt caatgtggac gggacgcata cggtatgcta aaggttgttt
     2581 catgattctc cgctcctttt tctctaccca ttatatgcca tctgataaaa aagaacaaac
     2641 attcgcccga ttgttttttg aagaattctg aaaattgatt gaaaagaaag acaggcaaca
     2701 tctttactat ttacaagcag aaaagctata atgaatacat tgaaaattgg aagcagaaag
     2761 tgagcgtaca taatgacgga tttcaaagag attattctaa cacacaaatt atcggcatta
     2821 ttttcgaaag cctcttttgg aatcgaaaaa gaaagccaaa ggataacgga cgaaggcaca
     2881 atcgccaaaa ctaatcatcc gacaatattc gggaatcgca gtttccatcc gtacatacag
     2941 actgatttcg cggagagtca accggaattg atcactccac cgatgcaatc gatcgaagaa
     3001 atgcatgaat ggctgatggc tatccatgac gttgttctgc ggtctttacc cgaaggtgaa
     3061 tacctgttgc cttgcagtat accacctgct atgccggcaa gtgaagaaat taaggtagcc
     3121 aaattggaca atgagagtga tgtcgcttat cgggaatatc tagtatcagt gtacggaaac
     3181 aagaagcaga tggtcagtgg cattcatttc aatttcgaat tgaatcctgt acttatcaaa
     3241 gagcttcatc agctttccgg ttccgtcagg acgcttaggg agttccaatc ggatgtatac
     3301 ttgaagatgg ctcataattt tattcgttac caatggatca tgacctacct tctgggcgga
     3361 tcgatatcag ctgaccgttc gtactttgaa aaagaatcac agcatgatct gccgttggat
     3421 cagtatacaa gaagtatccg cagcagcaag tacggatatg tcaataaagc ggacgtgcat
     3481 gtttcttttg aatcgattga tgcctatgtg caggatatcg aaaaaatggt gacaaccggt
     3541 aagcttattg ccgaaaaaga attttattcc actgtccgtt tcaggggggc caacaaagcg
     3601 cgtgatttgc tgacgaacgg aatcgcctat ctcgaattca ggctgttcga tctgaaccca
     3661 tttgctgagt ttggcatgca taaagaggat atgtacttca tccattattt cttgctctat
     3721 ttattgtgga tcgatcagga tgccagtgag gcagagatgc agctcggtaa agaaatgaat
     3781 tacagcactg ctttggagaa tcctttgcag ccatccgcat tccaagcgga aggactatcc
     3841 gtcctggaag gtatgctgca gatgctggaa gcgatcgatg cagaagagaa aatcagcgca
     3901 atcgtcaaag aaaaaatcga agcattc
//
LOCUS       DAOMUJ010000131         3899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6562_length_3899_cov_2.569459, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000131 DAOMUJ010000000
VERSION     DAOMUJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_3899_cov_2.569459"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLT73_04475"
     CDS             complement(<1..247)
                     /locus_tag="PLT73_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRM19667.1"
                     /translation="MPDNLSQIKEQLECHLGQKVMLTAQAGRKRKTERKGILRETYHA
                     VFVVDLDQNENAFERVSYSYADVLTEAVEILFYDEEKC"
     gene            complement(510..1409)
                     /gene="rsmA"
                     /locus_tag="PLT73_04480"
     CDS             complement(510..1409)
                     /gene="rsmA"
                     /locus_tag="PLT73_04480"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRM19668.1"
                     /translation="MSALEHQKDIATPSRTNAILKKYHLTMKKSLGQNFLVEPNILTK
                     MVAAAGIDKNTNAIEIGPGIGALTERLAREAKKVVAFEVDRRLAPILKTELAEYDNIE
                     IIFQDILQANVPQVIEEHFEPAERLVVAANLPYYITTPIIMNFIETDLPIDTFVMMMQ
                     KEVAQRMTAKPGTKAFGSLTIAIDYYTEAEIAFIVPKTVFVPQPNVDSAILKLQRRAE
                     PKVKVTDEKFFFDLVRAGFNQRRKTLWNNLQSRYGKTEEVKAALTAGLEEAGIEPSKR
                     AEALSIEQFADLANALVKYLDVK"
     gene            complement(1406..2005)
                     /gene="rnmV"
                     /locus_tag="PLT73_04485"
     CDS             complement(1406..2005)
                     /gene="rnmV"
                     /locus_tag="PLT73_04485"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HRM19669.1"
                     /translation="MKIEEIIVVEGKDDTKRIKEAVEADTIETNGSAINEATLQLIAH
                     AQATRGVIVFTDPDYPGGKIRATIVERIPGVKHAFLRKDDAQSPKGGSLGIEHASPEN
                     IRAALKNVYTQRQVETSDIDRKFLDEWHLIGHADSGRYRELLGDVLGIGHTNGKQLLK
                     RLRMFQLERVDVDAAMTAILKELDEKDSLTAQRQEEENR"
     gene            complement(2002..2784)
                     /locus_tag="PLT73_04490"
     CDS             complement(2002..2784)
                     /locus_tag="PLT73_04490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRM19670.1"
                     /translation="MDKLLFDTHTHLNVDAFADDAAAAITRAHEAGVGRFAVVGFDAD
                     TIKKSLELSAKYPEIYSIIGWHPTEAATYTAAVEDSLMALLEEKKVVAMGEMGLDYHW
                     DTAPHDVQSKIFRRQIAIAKELELPISIHMRDAIEDTYRILKEEDVRDIGGIMHSFSG
                     DVDYMKKFLDLGMHISISGVVTFKKATELHEVAKVVPIEKLLVETDAPYLAPVPFRGK
                     RNEPAYVRYVAEKIAELREQTFGEIALQTTKNANKLFGLDDK"
     gene            complement(2898..>3899)
                     /gene="metG"
                     /locus_tag="PLT73_04495"
     CDS             complement(2898..>3899)
                     /gene="metG"
                     /locus_tag="PLT73_04495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRM19671.1"
                     /translation="VTFGSDGIFTPEDYVNRINYDLANDLGNLLNRTIAMINKYFGGN
                     IPAALAAETAFDAELKAMAAEVTENYQKEMDNMQFSSALSETWRLISRANKYIDETAP
                     WVLAKDESKQAELGSVMAHLAETLRVVGILLSPFMTQAPFRMYEQLGLDFEKQGAWEN
                     VAFGTFPADVKVVEKGTPIFPRLVTEEEVAYIKEQMGGGTIPAEEETVAAEWDPTTTV
                     LTSEKEKQIKYEDFDKVELKVAEVIDCQKVEGADKLLKFRLDAGDEGHRQILSGIAQW
                     YPDPAYFIGKKVIIVANLKARKMKGEISQGMILSAEKDGILQVILAPESAANGSTVA"
BASE COUNT          859 a         1039 c          844 g         1157 t
ORIGIN      
        1 ggcatttttc ttcatcataa aataaaatct ctacagcttc ggttaaaaca tctgcataac
       61 tgtatgatac acgttcaaat gcattttcgt tttggtctag atcgactaca aatacagcat
      121 gatacgtttc tcgtaaaata ccttttcgtt ccgtcttgcg tttgcgtcct gcttgcgcag
      181 tcagcataac tttttgccct aagtgacatt ccagttgttc tttaatctga ctcaaattat
      241 ccggcattct gtttcacctc actgatttac agtataacac agataaatta aaataacaag
      301 tatatcacag aggacgtatg cacgcaacta tgaaacgaat aaatttttaa agaaatctta
      361 tcaacttttt tcacagtttt tagtaaacgt ccgacagaaa atcagcacaa cctccccttc
      421 acctttaatt tgaaaggggg gctgcgaggt ggggctgtct tctcgacggg gtctccggaa
      481 actctaatat caagcaagca cttggtcgct tatttaacat ccaaatactt gaccaaggcg
      541 ttggcaagat ccgcaaattg ttcgatgctc agggcttctg cccgttttga gggttcgatg
      601 cccgcttctt caagtccggc tgtcaaagca gccttgacct cttccgtttt gccgtagcgc
      661 gactgcagat tgttccagag cgtttttctg cgttggttga aaccggcgcg gaccaaatca
      721 aagaagaact tctcatccgt aaccttcact ttgggttccg ctctgcgttg cagcttcagg
      781 attgccgaat cgacattcgg ttgcggcaca aaaacggttt tcggaacgat gaaggcaatc
      841 tcggcttccg tatagtagtc gatcgcaatc gtcaatgaac cgaatgcttt cgttccgggc
      901 ttggcggtca tgcgttgtgc gacttccttt tgcatcatca tcacaaacgt atcgatcggc
      961 aaatccgtct cgatgaaatt catgataatc ggcgtcgtga tgtagtaagg cagattcgcc
     1021 gctaccacca atcgttcagc cggttcaaaa tgctcctcga tgacctgcgg aacattcgcc
     1081 tgcagaatat cctgaaaaat gatttcgata ttatcgtatt ccgcaagttc cgtcttcaaa
     1141 ataggcgcca gtcgtctgtc cacttcaaac gccactactt ttttggcttc tctggcaagg
     1201 cgctccgtca aggctccgat accgggaccg atctcgatcg cattggtgtt tttgtcgatg
     1261 ccggctgcag ccaccatctt cgtcaatata ttcggttcca ccaaaaagtt ttgccctagg
     1321 cttttcttca tggtcagatg gtatttcttt aagatggcgt tcgttctgct tggtgtcgct
     1381 atatccttct ggtgttctaa tgcactcatc tgttttcctc ctcctgccgc tgagcggtca
     1441 ggctatcctt ttcatccagt tcttttaaaa tggctgtcat cgctgcatca acatctactc
     1501 tttccagttg aaacatgcgc aggcgtttca ataactgttt tccgtttgta tggccgattc
     1561 cgagcacatc ccccagcaat tcacgatagc gccctgaatc ggcgtgtccg atcaagtgcc
     1621 attcatccag gaatttcctg tcgatatcgc tcgtttcaac ctgtctttgc gtataaacat
     1681 tcttcagcgc tgcccttatg ttctccgggg aggcatgttc gattcctaga cttccccctt
     1741 tcggagattg cgcatcatcc ttgcgcagga atgcatgctt gacgccgggt atccgttcca
     1801 cgattgttgc ccggattttg ccgcccggat aatccggatc ggtgaagacg atgacgccac
     1861 gtgtcgcttg cgcatgggca atcagctgca acgttgcttc attgattgcc gaaccgttcg
     1921 tttcgatcgt atcagcttca accgcttcct tgatccgttt ggtgtcgtct ttcccctcaa
     1981 ccactataat ttcttctatt ttcatttgtc gtccaaccca aataatttat tcgcgttttt
     2041 ggttgtctgc aacgcgattt ctccgaaggt ttgctcgcgt aattcagcaa tcttttcggc
     2101 tacatagcgt acataagccg gctcgttccg ctttccacgg aaaggaactg gtgccagata
     2161 cggcgcatcg gtttcgacca gaagcttctc aatcggaaca actttggcca cttcatgcaa
     2221 ttccgtcgct tttttgaaag tgacgacgcc actgattgaa atgtgcatgc ccaaatccaa
     2281 aaactttttc atatagtcca catcgccgct aaagctgtgc atgatgcctc cgatgtcgcg
     2341 cacatcctct tccttcagaa tgcggtacgt atcttcgatg gcatcacgca tgtggatgct
     2401 gatcggcagt tccaattctt tggcgatcgc gatttgtctt ctgaatattt tcgactggac
     2461 gtcatgcggc gctgtgtccc agtgatagtc cagacccatt tcccccatgg caacaacctt
     2521 cttttcttca agcaacgcca tcaggctgtc ttccactgcc gcagtgtaag tcgctgcttc
     2581 tgtagggtgc cagccgatga tgctgtagat ttcgggatat tttgcgctca attcaagcga
     2641 tttttttatt gtgtccgcat cgaagccaac taccgcaaat ctgccgacac cggcttcgtg
     2701 agcccttgtg atggccgctg cagcatcatc cgcaaaagca tccacgttca gatgcgtatg
     2761 tgtatcaaat aataatttat ccatagattc ctttccggtt cctgcgggaa ggctaagcct
     2821 gccctgattc ttctgcatct aaaatggaca aaggagtccg ggacaactgt cccaggctcc
     2881 ttgaatcaca aaaaccttta tgccactgtt gaaccatttg ctgccgattc aggtgcgagt
     2941 atcacttgca gtatgccatc cttttcagca gaaaggatca tcccttggct gatttcaccc
     3001 ttcattttgc gcgctttcag gttagcgacg atgatgactt tcttgccgat gaaatacgca
     3061 ggatccggat accattgtgc aatgcccgac aggatttggc gatgaccttc atcccccgca
     3121 tccagacgga atttcagcaa cttgtccgcg ccttcgactt tctggcaatc gatgacttca
     3181 gcaaccttca actcgacttt atcgaagtcc tcatatttga tctgcttttc tttttcagaa
     3241 gtcagcacgg tagtcgttgg atcccactct gcagcaactg tttcctcttc tgcggggata
     3301 gttccgccgc ccatttgttc cttgatgtag gcgacttcct cttctgtaac gagacgcggg
     3361 aagattggtg tgcctttttc aactaccttc acatccgccg ggaacgttcc gaaagctacg
     3421 ttttcccaag cgccttgttt ttcgaagtcc aatccaagtt gttcatacat tctgaacggc
     3481 gcttgcgtca tgaatggaga cagtaggatg ccgacgacgc gcaaggtttc agccaaatgc
     3541 gccataacgc ttcccaactc cgcttgcttg gactcgtcct tcgccaagac ccatggagcg
     3601 gtctcatcga tgtatttgtt cgcgcgggaa atgaggcgcc atgtttcgga caaggccgaa
     3661 ctgaactgca tgttgtccat ttccttctgg taattctcgg tcacttccgc tgccatcgct
     3721 ttgagttccg catcgaaagc cgtttccgct gctagtgccg cagggatgtt acccccgaaa
     3781 tatttgttga tcatggcgat cgttctgttc agcaagttgc ccagatcatt ggccaaatca
     3841 taattgatgc ggttcacgta atcctcaggt gtgaagatgc cgtcgctgcc gaaggtcac
//
LOCUS       DAOMUJ010000132         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6638_length_3865_cov_2.728609, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000132 DAOMUJ010000000
VERSION     DAOMUJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_3865_cov_2.728609"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..774)
                     /locus_tag="PLT73_04500"
     CDS             complement(133..774)
                     /locus_tag="PLT73_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRM19672.1"
                     /translation="MPANMEHEIYRQIEETKLLPLYTATDLYCLERLEEILVKNEVRF
                     IEVTFRSDLALDAIRKLAQSGELIVGAGTVRTLKEAKDAVENGAKFIVSPAIVPDVIE
                     YCIGQDIPVFPGTATPGDIQRATEYGIKVVKFFPADIYGGLKAINALSGPFYDVKFLP
                     TGGINADNFMDYVENDHIVAVGGSFIISEAVIKKDDGKTADKILKTLVDKIRA"
     gene            complement(807..1874)
                     /gene="uxuA"
                     /locus_tag="PLT73_04505"
     CDS             complement(807..1874)
                     /gene="uxuA"
                     /locus_tag="PLT73_04505"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRM19673.1"
                     /translation="MKWGFRWYGEKGDTIPLDHVRQIPGINGVVGTLLNKLPGDVWEV
                     SEIQELKESVEKGGLELLGIESVAIHDAIKAGTPERDQYIENYITTIKNLSACDVRMI
                     CYSFKPIFGWAKTDLFYENKDGSFSLVYDQAVVDKMEPADMYKLIHSQSKGFSLPGWE
                     EERLNKFSKLVEMYEGVTEEILFENLSYFLKKIMPVCEEMDVKMAIHPDDPPFELFGF
                     PRITKNLEDLKKILSIVDSPYNGLTLCTGSLGAEPNNDMVEIIHEVGNKINFVHFRNV
                     EFLGERKFRESAHLSTDGSLDMYAIMKALVDVGFDGVIRPDHGRTVFGEVAMPGYGLY
                     DRAMGITYLQGLLEAITKAKG"
     gene            complement(1904..2350)
                     /locus_tag="PLT73_04510"
     CDS             complement(1904..2350)
                     /locus_tag="PLT73_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002398171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM19674.1"
                     /translation="MLGIIVATHGTLSDGLKNSAEVIFGPTNNIKTANLNLGDDVQAL
                     GSKIKESIHEVDQGDGIIVLVDLVSASPYNQSVLVTNSLEKPLQDSVHIIGGVNLPML
                     LEAINHQLIGTQVDQAAKAIIAQGKSSISEWHVSMVTEDDEEDDTF"
     gene            complement(2387..3274)
                     /locus_tag="PLT73_04515"
     CDS             complement(2387..3274)
                     /locus_tag="PLT73_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19675.1"
                     /translation="MQQNEKFKDWLFRYQSFYRVRRTDKSKRRFLSALVADISEMRED
                     VQVIEYNRHEKYASRNVYVGDIEKADQIICTYYDTPIQHFGPYVLFDRKEQEKRTTSF
                     IVATSVLLLLAGIAGTLLYMRYASGTADLISVRTALIALAYGVYFYLFGKITKGLPDR
                     RTVVRNTSSVLALLAMINDTKVKDEKTAFAFVDEGSFGESGLEAMLTPKNKKAKIFYL
                     DCVGADAPLHVIGNDISKAKLSEMSIDHQSSDEKINYIFSAKISEKEQKTQFHLDKSE
                     LNKKNLNMENITKVMELCR"
     gene            complement(3370..>3865)
                     /locus_tag="PLT73_04520"
     CDS             complement(3370..>3865)
                     /locus_tag="PLT73_04520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM19676.1"
                     /translation="VNLARVDERLIHGQVMLTLSQRDGVNSIFVVDDVVAKDKFMKDL
                     YKSAGSRTGQKTIVMTEEKCKFYWDEFKFKEYSAILITKTVTGIYNLVKHGVPIKDLN
                     IGGIAKKGDDDILVTKSVYLNKADALKLKELNEEYGVENIYFQATPSSSSSSLADVLK
                     QFGL"
BASE COUNT         1006 a          953 c          685 g         1221 t
ORIGIN      
        1 ttttttttgt gatacggacc ataattgagc catcagtcct atttgacaaa gagccaatga
       61 atcacaacgc atacggctat tttttgcatt tctgctcaat actcctgagc atgcattgac
      121 cagcaaccaa ttttaagctc tgattttatc caccagtgtt ttcagtattt tatcggctgt
      181 ctttccatca tcttttttga tgaccgcttc agagatgatg aacgagccgc ctaccgctac
      241 gatgtggtcg ttttcgacat agtccataaa attgtcagcg ttgattccgc cggtcggcaa
      301 gaatttgacg tcatagaaag gaccgctcaa agcgttgatc gcttttaacc ctccgtaaat
      361 atctgcaggg aaaaacttca caaccttgat accgtattcc gtagcccgct ggatatctcc
      421 tggcgttgcc gttccaggaa atacagggat gtcctgtccg atacagtact cgattacgtc
      481 cggaacgatt gctggtgaaa caatgaattt tgcgccattt tcaaccgcat ccttcgcttc
      541 tttcaacgtc cgcacagtgc ctgcgccgac aatcaattct ccggactggg ctaacttcct
      601 gatggcatcc aaagctaagt cacttcggaa tgtcacttcg ataaaacgca cttcgttctt
      661 gactaaaatc tcttccaacc gctccagaca ataaagatca gtcgctgtat aaagaggcaa
      721 caatttcgtt tcttcgattt gtcgatagat ttcgtgttcc atatttgctg gcattttcaa
      781 ctctcgcttt ctggcatcgc ggaaatttat ccttttgcct tcgtaattgc ttcaagcaat
      841 ccttgcagat acgtaatgcc catcgcgcgg tcatacaatc cataaccagg cattgcgact
      901 tcgccaaaaa cagtacgccc gtgatcggga cggataactc cgtcaaatcc gacatctact
      961 aaagctttca taatggcata catatccaag gagccatccg tgctcaaatg tgcggactcc
     1021 ctgaatttac gctcgcctaa aaattcgaca ttgcggaagt gaacaaagtt gattttgttc
     1081 ccgacttcat ggatgatttc aaccatatca ttgtttggtt cagctccaag ggaacctgtg
     1141 caaagcgtaa gtccgttgta tggagaatca acaatgctga ggattttttt caggtcttcc
     1201 aaattcttcg tgatgcgcgg gaagccaaaa agttcaaatg gcggatcatc cgggtgaata
     1261 gccatcttga catccatttc ctcacaaacg ggcatgattt ttttcaagaa ataacttaag
     1321 ttttcaaata agatttcttc cgttacgcct tcgtacattt ctaccaattt gctgaattta
     1381 ttcagacgct cttcttccca accaggcagg ctgaagcctt tcgattggct gtggatcagt
     1441 ttatacatat cagccggttc catcttgtcc acgacagctt gatcgtatac aagcgagaag
     1501 ctgccatctt tgttttcata gaataagtct gtctttgccc aaccaaaaat cggcttgaag
     1561 ctgtaacaaa tcatgcgaac atcgcatgcg gacaggttct tgattgtcgt aatgtaattt
     1621 tcaatgtatt gatcgcgttc aggtgttcct gctttgattg catcatggat cgctacactt
     1681 tcaatcccca ataattccag accgcctttt tccacggact ctttcaattc ttggatttca
     1741 gaaacttccc atacatctcc aggcaacttg ttcaataacg ttcccacaac tccgtttatt
     1801 ccaggaattt ggcgaacatg atccaacgga atcgtgtcgc ccttttctcc gtaccatcta
     1861 aatccccact tcattcagca ttcccctttt ccttctattt ttcttaaaaa gtatcgtcct
     1921 cttcatcatc ttctgtaacc atggacacat gccattcgct gatgctgctc ttcccttgcg
     1981 caataattgc tttagcggct tgatcaacct gtgttcctat caattgatgg ttgattgctt
     2041 ccaacagcat cggaaggttt actccgccaa tgatatgcac agaatcttgc agtggtttct
     2101 ccagactatt cgtcacaagc accgattggt tatacgggct ggcgctcacc agatccacca
     2161 acacaatgat tccatcccct tgatctactt catggatgga ttcctttatt tttgagccta
     2221 acgcctgcac gtcatctcca agattcaagt tcgctgtttt gatattattt gtcggcccaa
     2281 aaatcacttc cgccgaattt ttaagtccat cgcttaacgt tccatgtgtc gcaacgataa
     2341 tccctaacat taataatcct cctttgtttt ggagaatgca cagcatttat ctgcacaatt
     2401 ccataacttt tgtgatattt tccatattta aattcttttt gttcaactcc gatttatcca
     2461 gatgaaattg cgttttttgc tctttttccg atatttttgc actgaagata taattgattt
     2521 tttcgtccga tgactgatga tcaatactca tttccgataa tttcgcctta gaaatatcat
     2581 ttccgatgac atgcagcggt gcatccgcac ctacgcaatc cagataaaat atctttgctt
     2641 tcttattttt gggcgtaagc attgcttcga gaccactttc accaaaacta ccttcatcca
     2701 caaaagcaaa agctgtcttc tcgtctttta cttttgtatc attgatcatc gccaacaaag
     2761 ccagaactga cgatgtattc cggaccacag ttcttcgatc aggtagtccc ttagtgattt
     2821 ttccgaacaa gtaaaaatag acgccatatg caagagcaat aagtgcggta cgaacagaaa
     2881 tcaagtcagc cgtacctgaa gcgtaccgca tataaaggag cgttcctgct atacctgcca
     2941 gaagcaataa cacggaagtg gcaacgatga aactggtcgt ccgtttttcc tgttcttttc
     3001 ggtcaaataa aacataagga ccaaaatgct gaatcggcgt atcataatac gtgcaaatga
     3061 tctgatccgc tttttcgata tcgccaacgt aaacgttcct tgaagcatac ttttcgtgtc
     3121 gattgtactc gatcacctgc acatcttccc gcatctctga aatatccgcc accaatgccg
     3181 agagaaaccg ccgtttgctt ttatccgtgc gacgcacacg ataaaaggac tgatatcgaa
     3241 atagccaatc tttaaatttt tcattttgct gcatagtatt tgccacctat catcttgctc
     3301 actattttca tgtttgagaa aagcagctat tgctgttact caataactgc ttcctcgctc
     3361 agatttattt tataacccga attgcttcaa tacatccgct aaactggagc ttgaagaaga
     3421 gggcgttgct tggaagtaga tgttttcgac accatattct tcatttaatt ctttcaattt
     3481 cagcgcatct gctttgttca gataaaccga ttttgtaacc agaatatcgt catcgccttt
     3541 tttagcgatt ccgccgatat tcaggtcctt gatcggaaca ccatgtttga ccaagttgta
     3601 gatgcctgtc actgtttttg tgatcaggat agcactgtac tctttaaact tgaactcatc
     3661 ccaataaaat ttgcactttt cttccgtcat gacaatcgtt ttttgtccgg tacggcttcc
     3721 cgcactcttg tataaatctt tcatgaattt atctttggca acaacatcat caactacaaa
     3781 gatcgaattg accccgtctc gttgggataa tgtcaacatg acttgaccat ggatcaatct
     3841 ttcatctaca cgcgctaaat ttact
//
LOCUS       DAOMUJ010000133         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6639_length_3865_cov_2.090289, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000133 DAOMUJ010000000
VERSION     DAOMUJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6639_length_3865_cov_2.090289"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..345)
                     /locus_tag="PLT73_04525"
     CDS             complement(64..345)
                     /locus_tag="PLT73_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-transcriptional regulator"
                     /protein_id="HRM19677.1"
                     /translation="MPTISEKQYDQLEPWFKLKATEFNKLGYENIQVDDIYRYFKEFS
                     WKHTVPPHYYQQIRDIMKTTVNHYFDFVALEAQVYKVSSLDEINFDDFL"
     gene            complement(380..727)
                     /gene="yajC"
                     /locus_tag="PLT73_04530"
     CDS             complement(380..727)
                     /gene="yajC"
                     /locus_tag="PLT73_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRM19678.1"
                     /translation="MDTVIMIAFYGVLFGVMYFILIRPQKKQAQKTQDMLSQIKPGDK
                     VVTIGGLHGIVEEINSADNTVVLDCEGIFLTFEKRAISRISKVATITTDDAIVEDFTA
                     TETEQTQEDNNEE"
     gene            complement(805..1950)
                     /gene="tgt"
                     /locus_tag="PLT73_04535"
     CDS             complement(805..1950)
                     /gene="tgt"
                     /locus_tag="PLT73_04535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM19679.1"
                     /translation="MTEPAIRYRLIKKEKHTGARLGEIITPHGTFQTPMFMPVGTLAT
                     VKTMSPEELKTMGSQIILSNTYHLWLRPGSDLVEEAGGLHKFMNWDRGILTDSGGFQV
                     FSLSQNRQITEAGVHFRNHLNGSKMFLSPEKAIHIENQLGADIIMSFDECPPYSESYD
                     YIKASVERTTRWAERGLEAHKKPDQQGLFGIIQGAGYKDLRLQSARELISLDFPGYSI
                     GGLSVGETKEEMNGVLDYLTPVMPENKPRYLMGVGAPDSLIDGVIRGIDMFDCVLPTR
                     IARNGTCMTSSGRLVIKNAKYERDFRPLDEKCDCYTCKNYTRAYIRHLIKADETFGLR
                     LTSYHNLHFLINLMKNVRQAIMDDNLLEFREAFVEEYGFNVENPKKF"
     assembly_gap    2019..2033
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2121..2876)
                     /locus_tag="PLT73_04540"
     CDS             complement(2121..2876)
                     /locus_tag="PLT73_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRM19680.1"
                     /translation="MPREIGLNTLAYMQKAADNKVEQKEILKEIKEMGFPFVEVRREY
                     ILSGKTEMKEIAQQAADLNLKVFYSVPAELFTAGALNAQLGSYFEEAALLNAVQLKLT
                     LGEFQGLTSVLAEKLRELLTNFSVHLTVENDQSIGKGSPDVLMAFIAEARKASLDIGL
                     TFDTGNFVYIDIDPFVAANAMNEAVTYIHIKNVGYAAEGIVLSGLETGLVDMRRLLSC
                     FPDSVPASIEYPCGGGEEATETMRADRNKLRSW"
     gene            complement(2901..3551)
                     /locus_tag="PLT73_04545"
     CDS             complement(2901..3551)
                     /locus_tag="PLT73_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19681.1"
                     /translation="MTSLTFLLPILLPILFLVFFLSVLISIVKRINRLAENARLRAGT
                     SQATVLPQTVRQSFDGAGNENAKQLIKKMATLKPEQVYGFFQERLPEKYLREVSRILT
                     HRNWRREILIFVNRENLWELLLRTNPSKKGSDHNDAKPAAEIDGEEAFTDYNMDPTVG
                     LADDFTVFSEDAKELKTAKRMEKSTIQNRKKRSSNQKALMRAVIAKEILDRPDFDR"
     gene            complement(3548..>3865)
                     /locus_tag="PLT73_04550"
     CDS             complement(3548..>3865)
                     /locus_tag="PLT73_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HRM19682.1"
                     /translation="DVDVGRNVGAKLQAEQAEADKRVAQAKAEERRAFAVAEEQEMIA
                     EVQRMRAKVVEAEAEVPLALAEALRNGNIGVMDYYKMKNIIADTEMRSSISEFPADRS
                     EPE"
BASE COUNT          940 a          968 c          773 g         1169 t
ORIGIN      
        1 agaaaatcag gtattcaaaa aagcacaata ctctgcagta ttgtgctttt tacgggaaac
       61 gatttatagg aaatcatcaa aattgatttc atctaatgaa gagaccttat agacttgcgc
      121 ttctaaagca acaaaatcaa agtaatggtt taccgtcgtt ttcatgatat cccggatctg
      181 ctggtaataa tgtggaggga cagtatgctt ccaagaaaat tccttgaaat agcgatagat
      241 atcgtcgact tggatgtttt cgtagcccag tttattgaac tcagttgctt tcaatttgaa
      301 ccatggctcc aattgatcat attgtttctc tgatatagta ggcaatcgcc gcccctcctt
      361 tccgatggat caaatcgctt tactcttcgt tattgtcttc ttgtgtttgc tctgtttctg
      421 ttgctgtgaa atcttcgaca atcgcatcat ccgttgtgat tgtggcaacc ttcgaaatgc
      481 gtgaaatagc gcgtttttca aaagtcagaa aaatcccttc gcaatccaat acgactgtgt
      541 tatcagcaga attgatttct tcaacaattc cgtgcaagcc gccgatggtg actactttat
      601 cccctggctt gatttgactc agcatatcct gtgttttttg tgcttgtttc ttttgtggtc
      661 tgatcaggat gaaatacatt acgccgaaca ataccccata aaaggcaatc atgattactg
      721 tatccataca ttttcctcca tctgtggatt atttctatct atcctccact ttaccaaact
      781 ttaagttaga ctaccaccca aaatctaaaa cttcttcgga ttttcaacgt tgaagccgta
      841 ctcttccacg aatgcttccc tgaattccaa tagattatca tccatgatgg cttggcgcac
      901 atttttcatc aagtttatca agaaatggag attatgataa gaggtcaagc gcaagccaaa
      961 tgtttcatca gctttgatta aatgacgaat gtacgcacgt gtataatttt tgcatgtata
     1021 gcaatcgcat ttttcatcca atggtcggaa atcccgttca tatttcgcat tcttgatgac
     1081 caaacggccg ctgcttgtca tacaagtacc atttcgcgct attcgagtcg gaagcacaca
     1141 gtcgaacatg tcgattccgc ggatgactcc atcgatcaag gaatccggtg cacctacgcc
     1201 catcagatac ctcggtttgt tctccggcat gactggcgtc aggtaatcca gcacaccatt
     1261 catctcttcc ttcgtttcgc cgaccgaaag tccaccaatc gaataacctg gaaaatccaa
     1321 agaaatcagt tctctggcgc tctgcagcct taaatccttg taacctgcac cttggatgat
     1381 gccgaacaag ccttgttgat cgggcttttt gtgggcttcg aggcctcgct ctgcccatct
     1441 tgtcgttctt tcgacagacg ctttgatgta atcatagctt tcgctgtatg gagggcactc
     1501 gtcaaagctc atgatgatat cggcacctag ctgattttcg atgtggatcg ctttttcggg
     1561 tgacagaaac attttcgaac cattcagatg attgcggaaa tgtacgccgg cctcggtgat
     1621 ctgtctgttc tggcttaacg agaagacttg gaagccacct gaatcggtca agatccctct
     1681 gtcccaattc atgaatttat gcaggcctcc tgcttcttcc accaaatcgg agcccggtct
     1741 gagccaaagg tgatacgtgt tgctcaggat gatctgggag cccattgtct ttaattcttc
     1801 aggagacatt gtctttacgg tggccaaagt gcctaccggc atgaacatag gtgtctgaaa
     1861 agtaccgtgg ggcgttatga tctctcccaa acgcgcacct gtatgttttt cttttttgat
     1921 aaggcgataa cgaatcgctg gttcagtcaa tgtattcact tctttcttat agatatccgg
     1981 caatcagtat accatagtca cacactattt tcttgtgtnn nnnnnnnnnn nnncggattg
     2041 cattttttcg ttattcgcac gactcacgga tgactttcac ccacccctcg aaacgtccct
     2101 aacggtttag tcgaagtgcg tcaccaagag cggagcttat tgcggtcagc gcgcatggtt
     2161 tcagttgctt cttcgccgcc tccacatgga tattcgatgc tggccggcac tgaatccgga
     2221 aaacacgaca aaagacggcg catatccacc aagccggttt ccagcccgga caaaacgatc
     2281 ccttcagctg catagccaac atttttgata tgtatatagg ttaccgcttc gttcattgca
     2341 ttcgctgcaa cgaagggatc gatgtcgata tacacgaaat tacccgtgtc aaaagtcaat
     2401 ccgatgtcca atgatgcttt acgggcttca gcaataaacg ccatcaggac atcagggctg
     2461 cccttcccaa tggattgatc attttcgacc gtcaaatgga ctgaaaaatt cgttaacaat
     2521 tcccgtaatt tttcagccag cacggatgtg agtccttgga attcccctaa agtcagtttc
     2581 agctggactg cgttcaaaag agcggcctcc tcaaaatagc tgcctagttg tgcattcagc
     2641 gcgcctgctg tgaatagttc tgctggtacg gaatagaaca ccttcaagtt caggtcagcg
     2701 gcttgctggg ctatttcctt catttccgtt ttaccggaca aaatgtattc tcttctgact
     2761 tccacaaaag ggaaacccat ttccttgatt tccttcaata tttctttttg ttccacttta
     2821 ttgtctgcag ctttttgcat gtatgctaaa gtgttcaatc cgatttctct gggcatccat
     2881 ctgtctcctt cactttccta ttagcggtca aagtccggcc tatcgagtat ttctttggca
     2941 atgacagctc gcatcaatgc tttttgattg ctggaacgtt tcttcctgtt ctgaatagtg
     3001 gatttctcca ttcttttagc agttttcaat tctttagcat cttcagaaaa gacggtgaag
     3061 tcatcagcca accccacggt aggatccata ttatagtccg taaacgcttc ttccccatcg
     3121 atttcagctg caggtttcgc atcattgtgg tcgctgcctt tttttgacgg attcgtccgc
     3181 aacagcaact cccacaaatt ttcgcggttt acgaaaatca ggatttccct ccgccaattt
     3241 ctgtgggtca aaatccggct gacttcccgc aaatattttt cgggcaatcg ctcctgaaaa
     3301 aagccataga cctgctcagg cttcaatgta gccatttttt tgatcagttg cttcgcattc
     3361 tcatttccgg caccatcaaa tgactggcga actgtctgag gcaagacagt tgcctgggaa
     3421 gtcccagctc gaagtctggc attctccgcc aaacggttta tccgtttaac tatcgatatg
     3481 agcacagaga gaaagaacac caggaaaaga atcggcaaca agatcggtaa aagaaaggtt
     3541 aggctagtca ttccggttcg cttctatctg ccggaaactc tgaaatgctg ctcctcattt
     3601 cagtatccgc tatgatattt ttcattttat aataatccat cacgcctata tttccgttgc
     3661 gcagtgcttc agccaacgcc aacggcacct ccgcctcggc ttcgaccact ttcgcccgca
     3721 tcctttgcac ctctgcgatc atttcctgtt cttctgcgac cgcaaaagcg cggcgctctt
     3781 ctgctttggc ttgcgcaacc cgcttatccg cctctgcttg ttccgcctgc aatttggcgc
     3841 ctacgttacg gccgacatca acatc
//
LOCUS       DAOMUJ010000134         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6681_length_3850_cov_2.110145, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000134 DAOMUJ010000000
VERSION     DAOMUJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_3850_cov_2.110145"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1531
                     /gene="uvrB"
                     /locus_tag="PLT73_04555"
                     /pseudo
     CDS             1..1531
                     /gene="uvrB"
                     /locus_tag="PLT73_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710575.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
     gene            1671..1940
                     /gene="rpsO"
                     /locus_tag="PLT73_04560"
     CDS             1671..1940
                     /gene="rpsO"
                     /locus_tag="PLT73_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRM19683.1"
                     /translation="MAISKELKNEIIKEYAIHEGDTGSPEVQIAVLTYEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRNKDVARYRELIQRLGLRR"
     gene            2164..>3850
                     /gene="pnp"
                     /locus_tag="PLT73_04565"
     CDS             2164..>3850
                     /gene="pnp"
                     /locus_tag="PLT73_04565"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRM19684.1"
                     /translation="MSEKKVFQMDWAGRLLQVEIGQLAKQANGAVLVRYGDTVVLTAA
                     VGSREPKDTDFFPLTVNYEEKMYAVGKIPGGFIKREGRPSENATLTARLIDRPIRPMF
                     PEGFRNEVQITNVVMSVEQDCAPEMAAMFGSSLALAISDIPFYGPIAGVNVGRVDGEY
                     VLNPTAPQNEASDIELTVAGSRVAINMVESSAAMVNEEDMPGALMFGHKAIQELCDFQ
                     DKIVAEVGKEKMTVKLLTLNPELVAEVTTAYGEKMTTAIMTEEKLAREAAIEDVKAEA
                     ILFFNEKYLDDANFAQISKEVRQIVEDMEKDEVRRLITVDKIRPDGRKIDEIRSLASE
                     VGLLPRVHGSGLFTRGQTQALSTCTLAPLGEHQIIDGLGMVDSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALKQVIPTEAEFPYTIRLVSEVLESNGSSSQASICAS
                     TLAMMDAGVPIKAPVAGIAMGLVMEGENYTVLTDIQGLEDHLGDMDFKVAGTSEGITA
                     LQMDIKIQGITEQILTEALMQAKKARMEILAQLTSTIAAPREELSQYAPKIEMM"
BASE COUNT         1128 a          822 c          985 g          915 t
ORIGIN      
        1 acaaagagca tacgctatcg ttaaggcaag gaatggagat ggaacggaat gaactattgc
       61 gcaggctggt tgaaatgcag tttgaacgca atgatatcga ttttcggcgt ggaacgttcc
      121 gtgtgcgcgg ggacgttgtt gaaatttttc tggcatcccg cgacaacgag gccattcgag
      181 tcgaattttt tggtgatgaa atcgatcgga tcagagaagt ggatgtgctg accggagaaa
      241 tcaagaacga tgtcgaccat tttcctgttt tcccagccac tcactttgtg gcgaatgaag
      301 aaaaaacctt gcatgccgtc gatatgattc gggcagaatt gaaagatcgc ctgaaggtgc
      361 tgcgcgctga aaataaacta ttggaagccc aacggttgga gcaacgcacc aactacgaca
      421 tggaaatgtt gatggaaatg ggatattgca acggaatcga aaattattcc agacatatgg
      481 acgggcgtgc tcccggagaa cctccgtata cgttgcttga ttttttcccg gatgattatt
      541 tgaccgtcat cgacgagtcg cacatcacga tgtctcaaat tcgcggcatg tacaacggtg
      601 accgttcgcg aaaggaacag ttggtggcgt acggcttccg tctcccgagt actttggaca
      661 atcgcccatt gacgttgaag gaattcgagg agcgggtcaa ccagatcatt tatatttcag
      721 cgacccctgg cccatacgag tatgaacaaa gcccatatgt tgcccaacag attatccgtc
      781 ccaccggttt attggatccg attgtggaag tgcggccaat caaaggtcag atcgatgatc
      841 tgatcagtga aatcaatttg cgcgtcgaac ggaatgagcg ggtcttcatc acaactttga
      901 cgaagaagat gtccgaagat ttgactgatt atctgaagga agtcggcatc aaggtaaatt
      961 atctgcacag cgaaatcaaa acgatggagc gcgcggaaat catccgtatc tccgtttggg
     1021 agaatttgat gtgttgatcg gtatcaatct gttaagagaa gggctggatg ttcccgaggt
     1081 ttcgctcgtt gccatcttgg atgccgataa agagggtttt ctgcgcagtg aacgttcact
     1141 tgttcagaca attggtcgag cagcaagaaa cgagaacgga aaagtaatca tgtacgcaga
     1201 ccgcataaca gattccatgg agcgggcgat taacgaaacc aacaggcgcc gtgcgacgca
     1261 ggaagcctac aatgttaagc atggtatcac gccaaaaaca atcatcaaag aagtgcgtga
     1321 tcgcatccgt atctctcacg ctcctgaaga aggcgacggt gacgtaagca ttctaagcat
     1381 ctataaagca atgacgatgg aacaacgccg cgaagcactc gatcaactgg accgggatat
     1441 gcgggaagcg gcaaaagccc tcaattttga aaaagctgcc gaaatccgtg atatggtgtt
     1501 ggagctgaaa gccgaataca aaggattata aaaaaagtga ttttcacttg caatcggtaa
     1561 ggaattacga taaaatagtc cttgtattat atatacgacc ttcaacttgg ttaaacgatt
     1621 caccaacgtt ctactcagtt aaaggtaact ttatgaaaga ggtgaaaaaa atggcaattt
     1681 caaaagaact taaaaatgaa atcatcaaag aatacgctat tcacgaagga gatactggat
     1741 cccctgaagt gcaaattgca gttcttacgt atgaaatcaa ccacttgaac gagcatgcac
     1801 gcgttcataa aaaagaccac cattcttacc gtggtttgat gaaaaaagtt ggacaccgtc
     1861 gtaacttgtt ggcttacttg cgcaacaaag acgttgcacg ctaccgtgaa ctgatccaaa
     1921 gattaggttt gcgtcgttaa tttcgtggct aagacatatt aagcgagatt ccactgtgaa
     1981 tctcgcttat tttttgggat ttttcggaca atgagtgcaa aaatgctgag caatcagcta
     2041 taatggaatg aggctcccgc tctacagacc gctactatac aaatgtgagc ttataagcgt
     2101 cggtaggttg acatttgttt agtagttggt agatgggaag catcgcaaaa ggagaatgat
     2161 aacatgtcag aaaaaaaagt gttccaaatg gactgggcag gtcgtttgct gcaagtcgaa
     2221 atcggccaat tggctaagca agccaatggt gcggtattgg tcagatacgg ggatactgtc
     2281 gtattgacgg ctgctgtcgg ttcaagagaa ccaaaagata ccgatttctt cccattgact
     2341 gtgaactatg aagaaaaaat gtatgcagtc ggtaaaatac ctggaggatt catcaagcgt
     2401 gaaggtcgtc caagtgaaaa cgcaacattg actgcacgtt tgattgaccg tccgattcgt
     2461 ccgatgttcc ctgaagggtt ccgcaacgaa gtgcagatca caaacgttgt tatgtccgtt
     2521 gaacaggatt gcgcaccgga aatggctgcc atgttcggtt cctcattggc attggcgatt
     2581 tccgacattc cgttctacgg accgatcgct ggagtgaatg ttggccgcgt ggatggcgaa
     2641 tatgttttga atccaaccgc tccacaaaat gaagcatccg atatcgagtt gaccgtagcc
     2701 ggttcgagag ttgccatcaa catggttgag agtagcgctg cgatggttaa tgaagaagat
     2761 atgcctgggg cattgatgtt cggccacaaa gcaatccagg aattgtgcga tttccaagat
     2821 aaaatcgtag ccgaagtcgg caaggaaaaa atgaccgtca agcttttgac tttaaaccct
     2881 gaacttgtgg cggaagtgac cactgcttac ggtgaaaaaa tgacgacagc catcatgaca
     2941 gaagaaaaat tggctcgtga agccgctatt gaagatgtga aagctgaagc aatcttgttc
     3001 ttcaacgaaa agtatctgga tgacgccaac tttgctcaaa tctccaaaga agtacgccaa
     3061 atcgtggaag acatggaaaa agacgaagtg cgccgtttga tcacagtcga caaaatccgt
     3121 ccagatggcc gaaaaatcga tgaaatccgt tcattggctt ccgaagtcgg cttattaccg
     3181 cgcgtacacg gttccggctt gttcacgcgt ggacagacgc aagcgttgtc tacttgtaca
     3241 ttggctccac tgggcgaaca tcagatcatc gacggccttg gcatggtcga cagcaagcga
     3301 ttcatccacc attacaactt cccgcaattc tcggttggca gcactggtag agcaggaagt
     3361 cctggacgcc gcgaaatcgg ccatggtgct ttgggtgaac gtgcgttgaa acaagttatc
     3421 ccgacagaag ctgagtttcc ttacacaatc cgtttggttt cggaagtatt ggaatccaac
     3481 gggtcctctt cacaagcaag catctgtgcc agcacgttgg caatgatgga tgcgggtgtg
     3541 ccgatcaaag caccggtagc gggtatcgcg atgggcttag tgatggaagg cgaaaactat
     3601 acagttttga cggacatcca aggacttgaa gatcacttgg gcgacatgga cttcaaagtt
     3661 gccggaacaa gcgaaggtat cactgcgttg cagatggaca tcaaaatcca ggggatcaca
     3721 gaacaaatcc tgactgaagc gttgatgcaa gcgaagaaag cgcggatgga aatccttgct
     3781 cagctgacca gcacgattgc agccccacgc gaagaactgt cccagtatgc tccgaagatc
     3841 gaaatgatgc
//
LOCUS       DAOMUJ010000135         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6715_length_3829_cov_2.360360, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000135 DAOMUJ010000000
VERSION     DAOMUJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_3829_cov_2.360360"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1314)
                     /locus_tag="PLT73_04570"
     CDS             complement(178..1314)
                     /locus_tag="PLT73_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM19685.1"
                     /translation="MSFDRNQYLRELETIVNIDSGSRNPAGTREVAAFLEEKYRQQGF
                     LTELVSVGPEVGPVLVATNAQDEPIDLLFIGHTDTVFLDGEASRRPFKIEGERLYGPG
                     VYDMKGCSLLTAYLLENLPGKLKQSLNICVIHNPDEEISSFYSREVIIEKAKMSKFAF
                     VMEPSSADGSMIKERKGIEKLKIHFKGRPSHAGDAPQEGRSAINELAHWIVRMNTLID
                     YDAGVSVNAGTIQGGSVPNVVPEHAQMELDYRYSNPADVTAFFDMLEELQSHATEMEI
                     GINIEVIGKRPPMVDVPGAKALRSVFEDVGKQIDTFVSFKKSGGVSDANFTTSVGVPT
                     VCSIGLIGGAFHTDHEYVELDSIVQRLDLLTAVIAEMSRRKMFC"
     gene            1589..2407
                     /locus_tag="PLT73_04575"
     CDS             1589..2407
                     /locus_tag="PLT73_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM19686.1"
                     /translation="MANKDVFTFYNGVTIPSIGFGTWQIPNEEAYAAVTMALKNGYTH
                     IDTAHAYHNEENVGKAIRDFGIAREEVFVTSKLPAQVKDFDGALQHFEETMNNLGLDY
                     LDLYLIHAPWPWDEIGKDCTEGNIQVWKAMEQLYDEGRIRAIGVSNFSIKDLQAILDN
                     CDIVPMANQIPFYIGRDQKGLLAFCQTHKIVVEAYSPLATGAILDSPEIKQMAEKYGV
                     TSAQLSIRYCLEKDTLPLPKSTHEARIIENAQLDFNISPEDVAVLDAMKDIRAK"
     gene            complement(2409..2666)
                     /locus_tag="PLT73_04580"
     CDS             complement(2409..2666)
                     /locus_tag="PLT73_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19687.1"
                     /translation="MLTIGYIDSNSGERCLRRKSVNIPLGSSDERSLAEVSAGSYRRT
                     SVSVRLTGAAHSHGLLFFVKIIICKNKKILAMECVHCKDFF"
     gene            3022..>3829
                     /locus_tag="PLT73_04585"
     CDS             3022..>3829
                     /locus_tag="PLT73_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRM19688.1"
                     /translation="MIQERQPTMSGAEELSERLKYGLLAGFALLMGGVAFLFNSPREI
                     YEGNLTILASPANLLTDYMALTNVGSALFNVSLMTLQAIWIVRMADAKINGPVIAGIL
                     TIAGFAFFGKNLFNSMPISIGAFTYARVTKVPIHKSLLAALFGTALAPLVSELSFNLG
                     IGQPYGVFLGLGAGFVSGFLIPPLAHHFVTFTRGFSLYNVGFTCGIIGTVFISLMRNF
                     GFEIETVNILASGYNGPFSVLLYSLFSCMFLIGLQINGWSFHGLKRIIRHS"
BASE COUNT          971 a          968 c          866 g         1024 t
ORIGIN      
        1 aaaagtgctg ttcttccggt gtgcttcggc aagtatttcc gccgcggaac actgcgccat
       61 ccatcaaaaa aaacagcagc gcagaggacc tggaaagggc cttcgcgctg ctgttcaatt
      121 tatagtttct catatctgca tcgttggggc tcacgatatg gaaagacgat ctgtgcttta
      181 gcagaacatt ttccgccggc tcatttctgc tatgactgcc gtcagaagat ccaagcgttg
      241 cacaatggaa tccagctcca catattcgtg atcggtatgg aaagcgccgc cgatcagacc
      301 gatgctgcag accgtcggaa caccgacact ggttgtgaag ttggcgtcgg atacgcctcc
      361 cgatttcttg aacgaaacga acgtgtcaat ttgcttgccg acatcctcga agacgctccg
      421 caacgctttc gctcccggca catcgaccat aggcggacgc ttgccgatga cttcgatgtt
      481 gatgccgatc tccatttcgg ttgcatgact ctgcaattcc tccaacatat caaagaaggc
      541 cgtgacgtcc gcgggatttg agtaacggta gtccaattcc atctgggcgt gctcggggac
      601 gacattcggt acgctgccgc cttggattgt gcccgcattg acagacacac cggcatcgta
      661 atcaatcaaa gtattcatgc gaacgatcca atgagccagt tcgttgatgg cgctgcgccc
      721 ttcctgggga gcgtcgccgg cgtgggaggg ccggcccttg aaatggatct tcagtttttc
      781 gatgcctttc ctttccttga tcatcgaacc gtccgctgaa ctcggctcca tgacgaaggc
      841 gaacttggac attttggctt tctcgatgat cacttcccgg gaataaaagg aactgatttc
      901 ctcatccgga ttgtggatca cacagatgtt gaggctttgc ttcagttttc caggcaagtt
      961 ttcaagcaga taggccgtca acaggctgca gcctttcatg tcatagacgc caggcccata
     1021 aagccgctct ccttctatct tgaaaggccg tctgctcgct tcgccatcca ggaacacagt
     1081 atcagtgtgg ccgatgaaga gcagatcgat gggttcgtct tgagcattcg tcgccaccaa
     1141 taccggcccc acttccggac cgaccgaaac tagctcggtc aaaaaacctt gctgcctata
     1201 tttctcttcc aaaaacgccg ccacttcccg tgtacccgca ggatttctgc tgccactgtc
     1261 gatattgacg atcgtctcca attcacgtaa atactgattc ctatcaaagc tcatggccat
     1321 tcccccttgg cgaaattata acattcactc ctgtctaaaa tataccgttt tttaaaagcg
     1381 ttctcaaatt aaccctgaca tcgattgtcg ccatacatga cgaaaccaga tccaatcgca
     1441 tatttgcaga caaattccgg aataattctt ccgtatcccc tatccaattc taagctattt
     1501 tgaattttcg agctatatcc ttttacatct gccgctgact gctctaaact gaataatagt
     1561 aaacgttatc ataacaggag gttattgaat ggcaaacaaa gatgtattca ctttttataa
     1621 tggtgtgacg attccctcca tcggtttcgg gacatggcaa atccctaacg aagaagccta
     1681 cgcagcggtg acgatggctt tgaagaatgg ctatacgcac atcgatacgg cgcatgctta
     1741 tcacaatgag gaaaatgtcg gaaaagcgat ccgcgacttc ggcattgccc gcgaagaagt
     1801 gttcgtgacc agcaagctgc ctgcgcaagt aaaagatttc gacggcgcct tgcagcactt
     1861 tgaagaaacc atgaataatc ttggcttgga ttacctagat ctttacttaa tccatgcgcc
     1921 atggccatgg gatgagatcg gcaaggattg caccgaaggc aacatccaag tttggaaagc
     1981 catggaacag ctctatgacg aaggccggat ccgcgcaatc ggggtttcga acttctccat
     2041 caaagacttg caagcgatcc tggacaattg cgacatcgtc ccgatggcga atcagattcc
     2101 tttctacatc ggccgcgacc aaaaaggact gctcgctttc tgtcaaacac acaaaatcgt
     2161 tgtcgaagcc tattcacctt tagctacagg tgcgattctg gatagcccgg aaatcaagca
     2221 gatggctgaa aaatacggcg tgacgtcggc gcaattatcc atccgttact gcttggagaa
     2281 ggataccttg cctttaccga agtccacgca cgaagcgcgc atcatcgaga atgcgcagct
     2341 tgactttaac atttcgccgg aagatgtggc cgttctggat gccatgaaag atatacgcgc
     2401 aaaataaatc aaaaaaaatc cttgcaatgg acacattcca ttgcaaggat ttttttattt
     2461 ttacagatga taatttttac gaaaaatagc aatccatgcg agtgcgctgc tccggtgagg
     2521 cgcacactca ccgaagttct tcggtaactt ccagccgaaa cttcggcgag gctgcgttca
     2581 tcggatgatc ccagcggaat gttgaccgat ttcctgcgaa ggcaccgttc accggagtta
     2641 gagtcgatgt atccgattgt cagcatactt cttcccccta aatgatatta tcctctttct
     2701 aaatcgtagc ggtaaatatc cgaaagtata ctaatccgga ctgaagcggt tcaaaagtac
     2761 gcctcttttg aacaggtatc tccttttgta ccaaaaattc agcacaaatt gttccaattg
     2821 tgtatttact attggaatat tagtggattt tagtaaaatt gaaaataaat attgtgagag
     2881 tcggtttggc cctgtataat taaatgtaag ttaacgttta catgatttta taagcttgaa
     2941 tttacttcct taagacaaac agcaatttcc atatttcatt ttcattaatc agtttcaaca
     3001 caataagatt gggggacact tatgatacag gaaagacagc ctactatgag tggcgcagag
     3061 gaactctccg agcgattaaa atacggttta ttggccggct tcgccttatt gatgggcgga
     3121 gtggctttct tattcaacag tccaagagaa atttatgaag gcaaccttac catcttggcc
     3181 tctccggcta acctgttgac ggactacatg gctttgacga acgttggatc ggcattgttc
     3241 aacgtttcac tgatgacgct gcaagccatc tggatcgtcc ggatggcaga tgcaaagatc
     3301 aacggtccgg tcatagccgg tattttaacg attgcgggat tcgctttttt tggcaaaaac
     3361 ttattcaact ccatgccgat cagcatcggc gctttcactt atgcaagggt cacaaaagta
     3421 ccgatacaca aatctttgct ggccgccctt ttcggaacag ccttggcacc gctggtcagt
     3481 gagctctcct tcaacctagg catcggtcaa ccctatggtg tgtttctggg gctgggcgcc
     3541 ggttttgttt ccggcttcct gattccccca ctggcgcatc atttcgttac cttcacaaga
     3601 ggattcagcc tctataacgt cggcttcacc tgcggcatca tcggaacggt cttcatctct
     3661 ttgatgcgca atttcggctt cgaaatcgaa acggtaaata tccttgccag cggctataac
     3721 ggtccatttt cagtgctgtt gtattcactg ttcagttgca tgttcctgat tggcctgcag
     3781 atcaacggat ggagcttcca tgggctgaag cggatcatcc gccattcgg
//
LOCUS       DAOMUJ010000136         3788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6819_length_3788_cov_2.117332, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000136 DAOMUJ010000000
VERSION     DAOMUJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_3788_cov_2.117332"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    207..216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            313..2355
                     /locus_tag="PLT73_04590"
     CDS             313..2355
                     /locus_tag="PLT73_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM19689.1"
                     /translation="MEDNTQNKRKRKSHIPFRLNLLFFIVFSLFSAVIFRLGYLQIVR
                     GDEFEAIVKRTETTLVTESVPRGLIYDRDGNILVGNEPQHSITYTRGANTTAEEMAKV
                     AAALAGLINVSFEELTERDLKDYWIAINNEVMLKRLSDDEKLLPGSELYEVELAKITA
                     EDIAYSDEQKEIAAIFKRMNSAYALSTTSIKNKDVTDEELAQVSENLSGLSGVDVATD
                     WVRIYPESDLLKSILGGVTSEKIGLPSDKVATYLAKGYARNDRVGNSYLEQEYESVLS
                     GTKSQWETITNQSGEVVTREESYEGKAGDNLVLTIDIDFQKEIEQIATDFLNSNVDSY
                     NDRMYIVASDPNTGEILGMTGKQRSTSNEIVDDALGVINSSYGMGSAVKGATVLTGYR
                     TGVISTDNNVLVDEPLQFQGSNLKKSVFNPTIGSQVAINDIEALAVSSNIYMIKLAML
                     MGGQSTYEAGSTLNISPSVIDELRGYFSQFGLGVKTGIDLPNEGSGLKASERSADKAI
                     NQSFGQSDLYTTLSQYINTIANGGTRYAPQLVSEIRSTDANGALGALETTLETKVMNQ
                     VDVDAQAMERVQQGFYQVTHSENGTAYSTFGKDPNDVAGKTGTAEAFYDGNNDNLKGE
                     SVFNSTFVGYAPFDNPEITVTVVVPYLSTDKDWATPIAKKVFDAYFNTGDYATPAE"
     gene            complement(2453..2959)
                     /locus_tag="PLT73_04595"
     CDS             complement(2453..2959)
                     /locus_tag="PLT73_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19690.1"
                     /translation="MNKQKLRFISIGFLLSAVLLAGFQLFYPEALPGSTFNGEATADS
                     TDYQEKYESTLAELELQKQLNEAATSSAPSEGAEVASSAPAEVASTAESSAAPAANTP
                     VTFVITSGQPMSVVIDNLVSTGLITDRAVFEQYLNERNLVTKINIGEYQLSQDMGYEV
                     IADMITLE"
     gene            complement(2973..3338)
                     /locus_tag="PLT73_04600"
     CDS             complement(2973..3338)
                     /locus_tag="PLT73_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19691.1"
                     /translation="MEQWTIVIVLLTIAIILLFASFFVKDNDRKFVDEMSEYTLQLTE
                     EIHELKRRLIAVEEELGKEIPVSEDSQNIKKVHNLTKQHIITLYTSGRTFDEIAEQLS
                     VPITTVQLVVDNYIEQSIV"
     gene            3559..3708
                     /gene="rpmG"
                     /locus_tag="PLT73_04605"
     CDS             3559..3708
                     /gene="rpmG"
                     /locus_tag="PLT73_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM19692.1"
                     /translation="MRLNITLECTECKERNYLSKKNKRNNPDRLEVKKYCPRERRVTL
                     HRETK"
BASE COUNT         1072 a          780 c          941 g          985 t
ORIGIN      
        1 ttacaaacaa gggcagtaag aacgaaagga aaagcatatt tttttgcggg gatagggttg
       61 tgaaacgctt cagcatgctg cacattcctc cttaaaatga aatttttttg cgttcctgca
      121 gattcgcctg actatgagtg aacgctcggg atagttgaag cggaaatttc acaagtgctt
      181 ctgctatttt agcatatccc gctggtnnnn nnnnnnataa cactgtaaca aatattaagc
      241 taagatatat tgtaatgctt gctttttttg ctataatagg ttgtcgaact atggttttag
      301 gaaggtgcaa aattggaaga taatacccaa aacaaaagaa agagaaaatc gcatatccca
      361 tttcggttga acctgctctt ttttatcgtg ttctcgctgt tttcggcggt gatattccga
      421 ttgggatacc tgcagatcgt tcgcggcgat gaatttgagg ctatcgtaaa acgtacggaa
      481 acgacacttg tgacggaatc ggtgccgcgt ggtttgatat acgacagaga cggcaacatc
      541 ctagtgggca atgaaccaca gcattccata acctacacaa gaggagcaaa cacgacagcc
      601 gaggaaatgg caaaggttgc cgctgcgttg gcgggtttga taaacgtcag ctttgaagaa
      661 ctgacggagc gtgatctgaa ggattattgg atagccataa acaacgaagt gatgttgaag
      721 cgtctgtcgg atgatgaaaa gctgttacct ggttccgaat tgtatgaagt ggagctagcg
      781 aaaataacgg cggaagatat cgcttattcg gatgaacaaa aagaaattgc cgcaatcttc
      841 aagcgaatga acagcgcgta tgccttgtcg actaccagca taaaaaataa ggatgtaacg
      901 gatgaagaac ttgcccaagt gagcgaaaat ctgtccggtc tgtccggagt ggatgtcgcg
      961 acggattggg tcaggatcta tcccgagtcc gacttgctga agagcatatt aggaggggtg
     1021 accagcgaaa aaatcggttt gccgagcgac aaagtcgcca cctatttggc aaaagggtac
     1081 gccagaaatg accgtgtcgg gaattcctat ttggagcaag aatacgagtc ggtgctctca
     1141 ggcacgaaat cacaatggga aacgatcacg aatcaatcag gtgaggttgt gacgcgggag
     1201 gaatcctatg aaggaaaagc cggggataat ctggtcttga ccatcgatat cgattttcag
     1261 aaggaaatcg aacagatcgc taccgatttc ctgaacagca atgtcgattc ttacaacgac
     1321 cggatgtata tagtggcatc agatccgaat acaggcgaga ttctgggcat gaccggcaaa
     1381 cagcgctcga cctcaaacga aattgtggat gatgcattgg gagtcatcaa ttccagctac
     1441 gggatgggtt cggcggtgaa gggcgcgacg gtgctgaccg gatacaggac cggtgtcatc
     1501 agtacggata acaatgttct cgtcgatgaa ccgctgcaat tccaagggtc aaatctgaaa
     1561 aagtcagttt tcaacccaac catcggcagc caagtggcca taaacgatat cgaggcgtta
     1621 gccgtctcat cgaatattta catgatcaaa ttggcgatgc tgatgggggg acagtcgacc
     1681 tacgaagcag gaagtaccct gaatatttca ccgagtgtga tcgatgaact gcgcggttat
     1741 ttttcccagt tcggtttggg tgtgaagacg gggattgact tgccgaatga aggcagcgga
     1801 ctaaaagcca gtgagaggag cgccgataaa gctataaacc aatcattcgg gcaatccgat
     1861 ctctacacga ccctgtcgca atacatcaat accatcgcca atggcggcac ccgatacgct
     1921 cctcagttgg tttcagaaat ccgcagcacg gatgcgaacg gagcgctggg ggctttggaa
     1981 acgactttgg agacaaaagt gatgaatcaa gtcgatgtcg atgcgcaagc gatggagcgt
     2041 gtccagcaag ggttttacca agtaacccac agcgagaacg ggacggctta ttcgacattc
     2101 gggaaagatc cgaacgacgt cgccggcaag actgggacgg cggaagcttt ctatgacggg
     2161 aacaatgaca atctgaaggg tgagagtgta ttcaactcca cctttgtcgg ctatgcgccc
     2221 ttcgacaatc cggaaatcac cgtaacggta gtggttcctt atctcagcac ggataaggac
     2281 tgggctacac cgattgccaa gaaagttttc gatgcgtact tcaatactgg ggactacgca
     2341 acgccagcgg aataaccatc ggctgacgat ttcaaatgga cacaaagaag gagccggaac
     2401 aaggattgca atatccttgt tccggctcct tctgtctttg tagagtgggt cattattcca
     2461 gtgtgatcat atcggcgatg acttcatagc ccatgtcctg ggacaattgg tactctccga
     2521 tatttatttt cgtgacaaga ttccgttcat tcaggtattg ttcaaagaca gcccgatcgg
     2581 taatgagtcc cgtactgacc agattgtcga tgacgacgga catcggttgt ccggaagtga
     2641 tgacaaatgt gacgggagtg tttgcggcag gggcagctga actttcagcg gtactcgcga
     2701 cttcagccgg agcactggat gccacttcgg ctccttcact aggcgcactg cttgttgctg
     2761 cttcattgag ttgcttctgc aattccaatt ccgccaaagt actttcgtat ttctcttgat
     2821 aatccgttga atcggcagtt gcttctccgt tgaaggtgct gccaggaagg gcttccggat
     2881 agaaaagctg gaagccggcc aacagtacgg cagacaacaa aaaaccgatg gatataaaac
     2941 gaagcttttg tttgttcact ttattttctc ctctatacga ttgattgctc tatgtagtta
     3001 tcgacaacga gttgcacggt ggtgataggg acggacagtt gttctgcaat ctcatcgaat
     3061 gttcttccgc ttgtatacaa agtgatgata tgctgcttag tcaagttgtg tacttttttg
     3121 atgttctgcg agtcttcgga gacgggaatt tcttttccta attcctcttc cacagcgatg
     3181 agtcgtcttt tcaattcatg gatttcttcc gttaattgta atgtgtattc actcatctca
     3241 tctacgaatt tgcgatcatt atccttgacg aaaaatgatg caaacagcaa tatgattgcg
     3301 attgtcagca agacaatcac gatggtccat tgttccatct ttgtcacctc aattcttctt
     3361 tttgacgcct gataacattc taatgaaatt cgtttatttc tgcaacctta tctggaattt
     3421 aatcggaaga aagctgaaaa aatcgaatgt ccgcataact tgttcaaaaa atagtggcat
     3481 tattttttcg gaagtgatac aatgagaaac gcagaagatg attcgatctt caattttttt
     3541 atgtttggag ggaagaatat gcggttaaat attacattgg aatgtacgga gtgtaaagaa
     3601 cgtaattact tatcaaagaa aaacaaacgt aacaacccgg accgtcttga agtgaaaaaa
     3661 tattgtccac gtgaaagacg cgtgactttg catcgtgaaa caaaataatt aaaatgacaa
     3721 caggatatcc tgttgttttt ttatataact aggaaagtat ataattttcc ggctgtggaa
     3781 cgttgtgc
//
LOCUS       DAOMUJ010000137         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6848_length_3776_cov_2.804085, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000137 DAOMUJ010000000
VERSION     DAOMUJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_3776_cov_2.804085"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PLT73_04610"
     CDS             <1..1043
                     /locus_tag="PLT73_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRM19693.1"
                     /translation="SQNYNFSNVPLRQDITYSVEELYQSALIYSANGSTIALAEALAG
                     SEAKFVDLMKAQLDEWGVESYELYNATGLSNSDVAEENLYPGAPADGYNKMTARGVAM
                     VADHLIDDYPEVLETTAVPELAFQAGTSDEIMMHSYNLMLPGMFYYREGVDGLKTGTT
                     LESGASFTGTAVQDDMRIITVVIGADESTKRFKETGRLMDYAFSTFEKTTVATKGEAV
                     STQEPITVAKGKQTEVGLVYNEDLVTVTEKGAEELALTTVFTPAEESLNEDGEIEAPI
                     EKGTEVGSVAVTVEGDEFGYLDGATSEDVKVAVASSVEKANIFVRGGRVVGDLFGKAW
                     QTVSDFVGGFFD"
     assembly_gap    1302..1332
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1455..2756
                     /gene="serS"
                     /locus_tag="PLT73_04615"
     CDS             1455..2756
                     /gene="serS"
                     /locus_tag="PLT73_04615"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRM19694.1"
                     /translation="MLDVKFLRDGFEDTAEKLAARGVKTEELVTFKELDAQRREKIVE
                     TENLKQYRNGVSQEIAQLKRNKENADDKIAEMKEVGDKIKALDEELNEIEEKTTFIAT
                     RLPNLPHASVPVGADEEDNVEVRRWGTPRAFDFEPKAHYDIAEALDILDFERGAKVTG
                     SRFVFYKGLGARLERACYNYMLDLHTEEHGYTEMIPPYMANEASMFGTGQFPKFKEDV
                     FQLTDERNFTLIPTAEVPLTNYYRDEIMNEADLPVYFTAMSPSFRSEAGSAGRDTRGL
                     IRMHQFHKVEMVKFSKPETSYEELEKMTDDAEAVLRGLNLPHRTITLCTGDMGFSAAK
                     TYDIEVWIPAQDTYREISSCSNTEAFQARRAKIRYRNEETGKLEFVHTLNGSGLAVGR
                     TVTAILENYQNEDGSVTIPDALVPYMGGITKITKENASNKR"
     gene            2831..3373
                     /gene="msrA"
                     /locus_tag="PLT73_04620"
     CDS             2831..3373
                     /gene="msrA"
                     /locus_tag="PLT73_04620"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRM19695.1"
                     /translation="MRIVSESKLEKATFAGGCFWCMVKPFDSLPGIESVVSGYTGGHT
                     ENPTYEEVCSGTTGHTEAVQITFNPALFPYKDLVEVYWQQTDPTDAMGQFVDRGTSYR
                     PVIFYHTPEQKEIAEASKQALQDSGKFTKPIVTSIEPAQPFYEAEEYHQDFYKKSSAH
                     YHGYRSHSGRDSYIESHWKK"
BASE COUNT         1127 a          784 c          979 g          855 t
ORIGIN      
        1 tcagccagaa ctacaatttt tcgaatgtac cattgcgtca ggacatcacg tattctgtgg
       61 aagagctgta ccaatccgca ttgatctatt ccgcaaacgg atcgacgatc gctttggcgg
      121 aagctttggc cggttcggaa gcgaaattcg tcgatctgat gaaggcccag ttggatgagt
      181 ggggcgtaga aagctatgaa ctctataatg caactggctt gtcgaacagc gatgtcgcgg
      241 aagaaaatct atatccgggt gcaccagccg acggatacaa caaaatgaca gcgcgtggcg
      301 tcgcgatggt tgcggatcat ctgatcgatg attaccctga agttttggaa acaactgccg
      361 tgccggagtt ggctttccaa gcgggaacat ccgacgaaat catgatgcac agctataact
      421 tgatgttgcc tggcatgttc tattatcgtg aaggtgtgga cggtctgaag accggaacaa
      481 cactggagtc gggtgcatcc tttactggta cagccgttca ggatgatatg cggatcatca
      541 ccgtggtcat cggtgctgat gagagcacca agcgctttaa ggaaacgggg cgcttgatgg
      601 actatgcctt ctcaaccttt gagaagacga cggtagcaac aaaaggcgag gctgtttcca
      661 cgcaagaacc gattaccgtg gccaaaggga agcaaacaga ggtcgggctt gtctacaacg
      721 aagacctggt gaccgttacc gaaaaaggtg cggaagagct cgcgctcaca actgttttca
      781 caccggcgga agagagcctg aatgaggacg gcgagattga agcgcctatc gaaaaaggaa
      841 ctgaagtcgg gtccgtcgct gtgacggtcg aaggtgatga gtttggttat ctggacggcg
      901 cgacctccga ggatgtcaaa gtcgcagtgg caagcagcgt tgaaaaagcc aatatatttg
      961 tgcgcggcgg tcgtgttgtc ggagacctgt tcggaaaagc ttggcaaaca gtatccgatt
     1021 ttgtcggagg gttttttgac tgatcctgaa gagctgaggg tttaacggaa aaccgtcgca
     1081 ttattgacag cacagggttt gttcagtaaa atgagggtat cgaagcttag accaattgaa
     1141 tagagaaagc catgcatgtg accagtaaat gatgtgaagc gccccagaga gtcagtggtt
     1201 cggtgagaac tgacccttca cctgttgaat ccccacatga agcgaacaat gaaaacaaga
     1261 aattgactaa attgttccgg atccgtccgt taacgcaatg annnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nncagccaga cctgatcggg actggctgaa gttgggtggt accgcgaacg
     1381 ttaaagccat ttcgtcccag attattctag ggatggagtg gctatttttg tacaaataat
     1441 gaggaggaaa gcatatgttg gatgtaaaat ttttgaggga tggatttgaa gatactgcag
     1501 agaaattggc tgcacgtggc gttaaaacgg aagagttagt gacattcaag gaattggatg
     1561 cgcaacgacg ggagaaaatc gttgagacgg aaaacctgaa gcaataccgc aatggcgttt
     1621 cccaggaaat cgcgcaattg aaacgcaaca aagagaatgc ggatgataag attgccgaaa
     1681 tgaaagaagt cggcgacaaa atcaaagcat tggatgaaga actgaatgaa atcgaagaga
     1741 agacgacttt catcgcaaca agattgccta acttgccgca tgcctccgtt cctgtcggtg
     1801 cagatgaaga ggataacgtg gaagtaagaa gatgggggac gccgcgcgcg ttcgatttcg
     1861 aaccaaaagc ccactatgat atcgccgagg cattggatat tctggacttc gaacgcggcg
     1921 ctaaagtgac cggcagccgt ttcgtcttct ataaaggcct gggagcccgc ttggagcgcg
     1981 cttgctacaa ttatatgctg gatctgcata ccgaagagca tggctatacg gaaatgattc
     2041 cgccatacat ggcgaatgaa gcatccatgt tcggtacggg acaattccct aaattcaaag
     2101 aggacgtttt ccaattgacg gatgaacgca actttacgct gattcctacg gcggaagttc
     2161 ctttgacgaa ctattatcgc gatgaaatca tgaatgaagc agatttgccg gtctatttca
     2221 cagcgatgag tccgtctttc cgttctgaag ccggaagcgc aggacgcgac acaagaggac
     2281 tgatcaggat gcaccagttc cataaagtgg agatggtgaa attcagcaaa ccggaaactt
     2341 cctatgaaga gttggaaaaa atgacggacg atgctgaagc ggtgctgaga ggcttgaact
     2401 tgccgcaccg tacgatcacg ttatgcacgg gcgacatggg cttctccgca gccaaaacgt
     2461 atgatataga agtctggatc ccggctcaag atacctaccg ggaaatcagt tcttgttcaa
     2521 ataccgaggc attccaagcc cgccgcgcga aaatccgtta ccgtaacgaa gaaacaggga
     2581 aactggaatt tgtgcacacg ttgaatggct cagggctggc tgtcggccgc actgtaactg
     2641 cgatacttga aaattaccaa aacgaagacg gttccgtcac aatccctgat gcacttgtgc
     2701 catatatggg cggcatcaca aaaataacca aagagaacgc aagcaacaaa cgctgatcat
     2761 aagcaagata tgcgcgtaag cttctgggta tatgatacaa tcatttcata cgtaaacgca
     2821 tacgaaagga atgaggatag tgagcgaatc aaaattggaa aaagctactt ttgctggagg
     2881 ctgtttttgg tgcatggtca agccgttcga cagtctacca ggcatcgaat cagtcgtttc
     2941 cgggtacaca ggcggacaca cagaaaaccc tacttatgaa gaagtgtgtt ccggaacgac
     3001 cgggcatacg gaagctgttc aaatcacatt taacccagct ttgttcccgt acaaagactt
     3061 ggtggaggtc tactggcagc agacggatcc aactgatgcc atgggtcaat tcgtagatcg
     3121 cggcacttct tatcgtccgg taatttttta tcatacaccg gaacaaaaag agatagcgga
     3181 agcatcgaaa caagctttac aggacagtgg gaaattcact aagccaatcg tgacttcgat
     3241 cgaaccggcc caaccgtttt acgaagcaga agaatatcat caggatttct acaaaaaaag
     3301 tagcgcgcat tatcacggat accgttcgca ttcagggcga gattcgtata tcgaatcaca
     3361 ttggaaaaag tagaaaatgt gctgaaacta taatggaaag aaggtccatt ccgaagaaat
     3421 gactttggag tgaaccttct tttttgctgc aaatgaatgt tttattctat tgcagagagc
     3481 ttttgccgtc attatgcaat aaaaaaatat attcaatcaa aaaagacttg ccaaaaagct
     3541 ctgaaacagg taagattgtt attcgttggc agatcaaatg gacaaccgca aaagtatcca
     3601 aggtcgttga tgacagtatg aacttccagg ccaattcatt aataacattt ttaaaaaagt
     3661 tgttgacaaa ctggttgcgc aagtggtatc atatagaagt tgtcatgtga gagcgtgcaa
     3721 cacaattaga cctttgaaaa ctgaacaaag aatgaacgaa ccaaatgtgc agggag
//
LOCUS       DAOMUJ010000138         3731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6966_length_3731_cov_2.068009, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000138 DAOMUJ010000000
VERSION     DAOMUJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_3731_cov_2.068009"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLT73_04625"
     CDS             complement(<1..719)
                     /locus_tag="PLT73_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM19696.1"
                     /translation="MLCQHCGKKQASIHLYASVNGQRRQIDLCQNCYQEFKNAQSQQN
                     NPNPRSQGPVDPFSFGNLDSFFKQIQQNQEQNYQQPPASEVARGGKGGKGGLLDEYGI
                     NLTQLARDGGIDPVIGRDSEIARVIEILNRRTKNNPVLTGEAGVGKTAVVEGLAQKIV
                     DGDVPQKLLDKEVIRLDVVSLVQGTGIRGQFEERMQKLMDEIRANKQIILFIDEIHEI
                     VGAGSAEGSMDAGNILKPALA"
     assembly_gap    1060..1124
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1125..1329
                     /locus_tag="PLT73_04630"
     CDS             <1125..1329
                     /locus_tag="PLT73_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HRM19697.1"
                     /translation="LVQTASKFNSDINLEYKGKSVNLKSIMGVMSLGVGQGADVVITA
                     EGPDEADAMAGITETMVKEGLAE"
     gene            1331..3061
                     /gene="ptsP"
                     /locus_tag="PLT73_04635"
     CDS             1331..3061
                     /gene="ptsP"
                     /locus_tag="PLT73_04635"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRM19698.1"
                     /translation="MKNHLQGIAASDGIAIAKAYLLTEPDLSFEKRKVEDSEGEIARL
                     LQSFETAKSEVSAIRDKAAVSLGEEEAQVFDAHLMVLSDPEMIGSMNANIRDNGVNAE
                     SALSDVAGMFIGMFEAMEDNPYMQERAADIRDVTERVLSHLLGVKIPNPSTITEEVIV
                     VAKDLTPSDTAQLNRDYVKGFVTDIGGRTSHSAIMARSLEIPAIVGSKEITQIVKDGD
                     IIILDGLDGDVFINPDEETLAAYKKKAEEFSAMQAEWHKLKNANTVTADGKHIELAAN
                     IGTPKDLEGVVANGAEAIGLYRTEFLYMDSSDFPTEDEQFDAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLELPKEMNPFLGYRAIRICLAEPEMFRTQLRALLRASVYGKLRIMF
                     PMIATLNEFRAAKAMLEEEKANLLADGVEVADDIQVGIMIEIPAAAVLAHQFAKEVDF
                     FSIGTNDLIQYTMAADRMNQQVSYLYQPYNPAILTLIKHVIDASHAEGKWTGMCGEMA
                     GDQTAVPLLVGLGLDEFSMSASSVLKTRSLMKRLDSKEMEKLADKAINECTTVEEVIA
                     LVEEMKAKLV"
     gene            3280..>3731
                     /locus_tag="PLT73_04640"
     CDS             3280..>3731
                     /locus_tag="PLT73_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM19699.1"
                     /translation="MRIGMFTDSYFPQVSGVSTSIRTLKEELEAEGHEVIIFTTTDPK
                     ADEDEKNIIRLTSIPFLSFKDRRIAVKGMNKALREAKKQKIDIVHTHTEFSLGLTGKF
                     VGYMLQIPTVHTYHTMYEKYLHYIANGRVVKPKAVKIASRYFCNRTMG"
BASE COUNT         1038 a          730 c          879 g         1019 t
ORIGIN      
        1 cttgctaggg ctggcttcag gatattgcct gcatccatgc ttccttcagc gcttcctgct
       61 ccgacgattt catggatttc atcgatgaag aggatgatct gtttgttcgc gcggatttca
      121 tccatcagtt tctgcatccg ttcttcgaat tgaccgcgga taccggttcc ttggacgagt
      181 gagacgacat ccaagcggat cacttctttg tccagcaatt tctgagggac atcaccgtcc
      241 acgattttct gggcaagacc ttccactacc gcagttttgc cgacaccggc ttcccctgtg
      301 agtaccggat tatttttcgt gcggcgattc agaatctcaa tgacgcgtgc gatttcgctg
      361 tctcttccga tgacagggtc gataccgcca tcgcgggcca gttgggtcaa gttgatgccg
      421 tattcgtcca gtaagccgcc ttttccacct ttaccgccgc gggcaacttc cgaggcaggg
      481 ggctgttgat aattctgttc ttggttctgt tgtatttgtt tgaagaacga atccaggtta
      541 ccgaaagaaa aggggtctac aggtccttga gatcttgggt tcggattgtt ttgttgcgat
      601 tgagcatttt tgaactcttg gtagcagttc tggcataagt cgatttggcg tctttgtcca
      661 ttcacgctgg catacagatg aatggaggct tgtttttttc cgcaatgttg acaaagcatg
      721 gcaatcatcc tttctatatg gatcatagtt acatgtatat tatagtagta acggctaagg
      781 gacactcaca agaaggttca ctgaccaact ctgaccttat ggatccatta tacattgacc
      841 tattttgact ttcaagaggt ttgacttgga atgaagcaaa atattcattc cgggacggtt
      901 ttcagccaaa tgggggttgt attaaataag tgttatttca gcaattatga caaaaatttg
      961 aacgattcta tttaaaacag ttgtagtata aagcgaaaga tgttaatctt attttatgaa
     1021 agggttattt ttcgtacgtt tttccatttt tctgttatan nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattggt gcaaactgca
     1141 agcaaattca attctgatat taatttagaa tacaaaggta aatctgttaa cttaaaatcc
     1201 atcatgggtg taatgtcatt aggcgtaggc caaggcgctg atgttgtcat cactgcagaa
     1261 ggtccggacg aagcagacgc tatggctgga atcactgaaa caatggtaaa agaagggcta
     1321 gcagagtaat atgaaaaacc acttacaagg aatagcagct agtgatggta ttgcaattgc
     1381 gaaagcatat ttgcttactg aaccggatct gtcctttgaa aagcgtaaag tggaagattc
     1441 agaaggcgaa attgctcgcc ttttgcaaag ctttgaaacc gctaaatcag aggtaagtgc
     1501 aatcagagac aaagcggcag tgtcacttgg cgaagaagaa gcacaagttt ttgatgcaca
     1561 tttgatggta ttgtccgatc cggaaatgat cggatcgatg aacgccaaca tccgagacaa
     1621 cggtgtcaat gctgaatcgg cactttctga tgttgccggc atgttcatcg gtatgttcga
     1681 agcgatggaa gacaacccat acatgcaaga acgtgctgcg gatatccgcg acgttacgga
     1741 acgtgtgttg agccacttgt tgggtgtcaa aatccctaac ccatcgacaa tcactgaaga
     1801 agtgatcgtc gttgccaaag atttgactcc tagtgacaca gctcaattga accgtgatta
     1861 tgttaaagga ttcgttactg atatcggtgg acgcacatcc catagcgcaa tcatggcccg
     1921 ttctctggaa attcctgcaa tcgtagggtc aaaagaaatc acacaaatcg tcaaagatgg
     1981 cgatatcatc attttggatg gtttggacgg cgacgtcttc atcaatcctg atgaagaaac
     2041 attagctgca tacaagaaga aagctgaaga gttttctgca atgcaagcag aatggcacaa
     2101 actgaaaaat gcaaatacag ttactgcaga cggtaaacat atcgaattgg cagctaacat
     2161 cggaacgcct aaagatttag aaggcgttgt cgctaatggc gctgaagcga taggattgta
     2221 ccgtactgaa ttcctgtaca tggactcttc agacttcccg acagaagatg agcaatttga
     2281 tgcttataaa gctgttttgg aaggcatgga aggcaaacct gtcgttgttc gtacaatgga
     2341 cattggtggg gataaagaat tgccttattt ggaattgccg aaagaaatga atccattctt
     2401 gggttaccgt gcaatccgga tttgcttggc tgaaccggaa atgttccgta cacaattgcg
     2461 tgccttgttg cgtgcttccg tttacggtaa attgcgcatc atgttcccga tgattgctac
     2521 tctgaacgaa tttagagcag ctaaagcgat gttggaggaa gaaaaagcga atctattagc
     2581 cgatggcgta gaagtcgctg atgatatcca agtaggtatc atgatcgaaa tcccagcagc
     2641 tgctgtatta gctcatcaat tcgctaaaga agtagatttc ttcagtatcg gaacaaatga
     2701 cttgatccaa tacacaatgg cggcagaccg catgaaccaa caggtttctt acttgtatca
     2761 accttacaac ccagctatcc ttacgctgat caagcatgtt atcgatgctt ctcatgctga
     2821 aggcaaatgg actggtatgt gtggcgagat ggcgggtgac caaacagcgg ttccgttgtt
     2881 ggtgggtctt ggtttggatg agttctccat gagcgcatcc agcgtcctga aaacacgcag
     2941 cttgatgaaa cgtttggatt caaaagaaat ggaaaaatta gcggacaaag caatcaacga
     3001 atgtacgact gtcgaagaag tcatcgcatt agttgaagaa atgaaagcta aattagttta
     3061 agccgattca ggcgcaaaaa gaccgggcat gcctggtctt tttgcgtgcc agtgaattca
     3121 aagcgatttc ggaagctgaa tccatcattt ccgcaggctt gttcgtgtgg atatattttg
     3181 caagtagggc gtagggagga gtgacaaata tcagaatgtt ctatatactg aacatattga
     3241 gtcattataa atttgatggg attttttcga ggtgaagtca tgagaatagg catgtttaca
     3301 gattcgtatt ttcctcaagt gagcggcgtg tcaacatcaa tccgcacctt gaaagaggaa
     3361 ttggaggcag aggggcatga ggtcatcatt tttaccacca cagatccaaa agccgatgaa
     3421 gatgaaaaaa atataatccg tttgaccagt atcccgttct tgtctttcaa agatcgcagg
     3481 attgctgtca aaggtatgaa caaagcgctt agggaagcga agaaacagaa gatagatatt
     3541 gtgcatacgc atacggagtt cagtttgggt ttgacgggca aatttgtcgg ctacatgcta
     3601 cagattccta ccgtgcatac ctaccacact atgtacgaaa aatatctgca ttacatcgct
     3661 aatggaagag tggtgaaacc taaggcagtt aaaattgctt cccgctactt ttgcaatcga
     3721 acgatgggca t
//
LOCUS       DAOMUJ010000139         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_6971_length_3730_cov_2.045170, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000139 DAOMUJ010000000
VERSION     DAOMUJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6971_length_3730_cov_2.045170"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="panC"
                     /locus_tag="PLT73_04645"
     CDS             <1..795
                     /gene="panC"
                     /locus_tag="PLT73_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRM19700.1"
                     /translation="WKKEGLSVGFVPTMGYLHEGHQSLMQKAVSENDRVVVSIFVNPM
                     QFGPSEDLESYPRDLEKDASLCEASGVDLIFHPEPKEMYHSDFSSFVDMHGLTESLCG
                     KSRPAHFRGVCTVVTKLFNIVQPDCAYFGQKDAQQLAVIQQMVRDLNLDVTVIGCPII
                     READGLAKSSRNSYLSADERKAALVLSQALEVAKQLLANGEQNAAKIRNAISKTIQAE
                     PLAKVDYVELVDARTLQQVDSVERPVLVALAVYIGKTRLIDNFITE"
     gene            810..1196
                     /locus_tag="PLT73_04650"
     CDS             810..1196
                     /locus_tag="PLT73_04650"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRM19701.1"
                     /translation="MQLNMLKSKIHRAVVVQAELSYVGSITVDKDLLDAAGLIEYEKV
                     QIVDIDNGARFETYVIAGERGSGMICLNGAAARCVQVSDKIIIMAYCLMDELEAKEHK
                     PLVVFVDEQNAITTVTRYEEHGRLSM"
     gene            complement(1266..2225)
                     /locus_tag="PLT73_04655"
     CDS             complement(1266..2225)
                     /locus_tag="PLT73_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM19702.1"
                     /translation="MIQTKLAIIGVGHVGSQVLTDCSHLNLFSDIILIDSNKDTARGE
                     ALDHRHALAASYRTNSKIYAGDYADCADADIIIISAGVSEKPRPGEDMPPRALMGKEN
                     AIEIRKIMTGITRYTKDAIIILITNPVDTITYIAENEFNYPKGKIFGTGTTLDTFRYR
                     QIVAAHYQVDPKNVSGYIMGEHGSTAFPAFSSTTVGALTLSQSDALLMPAIPLDQEFV
                     SQEVVHTASDVFNWKGWTNSGIGEVAAALARAVMLDEKSIFPVTATVNGLYNCHGEAA
                     FSMPCIIGRNGLEKQIPLPLDNEEYEKLQLSIKDIQFTLQSVK"
     gene            complement(2229..2942)
                     /locus_tag="PLT73_04660"
     CDS             complement(2229..2942)
                     /locus_tag="PLT73_04660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM19703.1"
                     /translation="MFTILFDLDDTLYDTSRPFFQTLADYSFRNNYSNEASFALFREH
                     CDNAFDLFSSGELTLAESHIMRTQHTFADLGLPLTAEEAMHFQKSYQKRQGEIVLSPG
                     IERLLNELENRNIPIAVFTNGPEKHQMKKFNALNLERWVPPARIFISENIGCPKPYAE
                     AFAHVQESLNATPGELLFIGDTYETDIIGGQAAGWTTLWYNHRRKQEDEKMVLEPAFY
                     SVAEMHQAINDNISKRKEM"
     regulatory      3127..3281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            3511..>3730
                     /locus_tag="PLT73_04665"
     CDS             3511..>3730
                     /locus_tag="PLT73_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19704.1"
                     /translation="MGLFILAGCGNGTTEDSSAADGSAESDAFVVGMEAGYPPFNWTQ
                     NDDSNGAVKIDGADGYANGYDVQMAQKVA"
BASE COUNT          936 a          818 c         1023 g          953 t
ORIGIN      
        1 tggaaaaagg aaggtttgtc ggttggcttt gttccgacga tgggctatct ccatgaaggc
       61 catcaaagct tgatgcagaa agccgtcagc gaaaatgatc gggtggtcgt cagtattttt
      121 gtgaatccga tgcagttcgg cccatccgaa gatttggaaa gctatccaag agatctggaa
      181 aaggatgcct ctttgtgcga agccagcgga gtggacctga ttttccatcc cgagccgaaa
      241 gaaatgtacc attccgattt cagcagcttt gtggatatgc atggtctgac ggagagtcta
      301 tgcgggaaaa gcagaccggc ccatttccgg ggcgtctgca cagtcgtgac aaagctattc
      361 aacatcgtgc agccggactg tgcctatttt ggacagaagg acgcccaaca actggcggtt
      421 atccagcaga tggtccgcga cctgaatctg gacgtcaccg tcatcggctg cccgatcatc
      481 cgggaagccg atggattggc taaaagttcg cgcaacagtt acctgtcagc agatgagcgt
      541 aaggccgcac tggtgctgag ccaagcgctc gaagtcgcca agcagttgct tgcgaacgga
      601 gaacagaatg ctgccaaaat ccgcaatgcg atttcgaaaa cgatccaagc ggagccgctg
      661 gcgaaggtcg actatgtgga gttggtggat gcgcgtacgc tgcagcaagt ggacagcgtc
      721 gaacgacctg tattggtggc tttggcggtt tatatcggaa aaacgcgtct gatcgataat
      781 tttatcacgg aataagtggg ggaaaaacaa tgcaactcaa tatgctgaaa agtaaaatac
      841 accgcgctgt cgttgtccaa gcggagcttt cctatgtggg cagcatcact gtcgacaagg
      901 acttgttgga tgctgcagga ctgatcgaat atgaaaaagt ccagatcgtc gacatcgaca
      961 acggtgcaag attcgaaacg tatgtcatcg caggcgaacg cggctcgggc atgatatgct
     1021 tgaacggtgc cgccgcccgc tgcgttcagg tgtcggataa gatcatcatc atggcttact
     1081 gcctgatgga tgaactggaa gcgaaggaac acaagccgct tgtggtgttt gtggatgagc
     1141 agaatgccat cacaacagtc acgcgctacg aagaacacgg cagactcagt atgtagacta
     1201 tccgtacagg gacaaaaaag cgcaaccgca tccggccaat cggccatgat gcggttgcgc
     1261 ttttcctatt tcacggattg cagcgtaaat tgaatatctt tgatggagag ttgcagtttt
     1321 tcgtattcct cattatccag cggcaaaggg atctgtttct ccaagccgtt cctgccgatg
     1381 atgcaaggca tgctgaaggc cgcttcgccg tggcagttgt ataagccatt gacagttgcg
     1441 gtcacaggga agatgctttt ctcgtccagc atgacagcgc gggccaaggc cgctgcgact
     1501 tccccgatgc ctgaattcgt ccagcctttc cagttgaaga catcagatgc cgtgtggacc
     1561 acttcttggc tgacgaattc ctggtccaat ggtattgcgg gcattaataa agcatcgctt
     1621 tgggaaaggg tgagcgcacc cacagtcgtg ctgctgaaag ccgggaaggc ggtactgccg
     1681 tgttcgccca tgatgtagcc gctgacgttc tttggatcga cctgatagtg ggcagcaaca
     1741 atctgacggt agcggaaagt gtccaaagtc gttccggtgc cgaaaatctt gcctttcgga
     1801 tagttgaact cattttcggc gatgtaggtg attgtatcga ccggattcgt tatcaggatg
     1861 atgatggcat cttttgtgta ccgggtgatg cctgtcatga ttttgcgtat ttcaatggca
     1921 ttctctttgc ccatcaacgc tcttggcggc atatcttcac ccgggcgcgg cttctcactg
     1981 acgcccgcgg aaatgatgat gatgtcggca tcggcgcaat ccgcgtaatc gccggcgtat
     2041 attttactgt ttgtccggta gctggcggcc aacgcatggc ggtggtcaag agcctcgcct
     2101 cttgccgtgt ccttgttgct gtcgatcagg atgatatcgg aaaataagtt gaggtgactg
     2161 cagtcggtca atacctggga accgacatgg ccgaccccga tgatggctaa ttttgtctgg
     2221 atcataggtc acatttcctt tcgttttgag atgttgtcat ttatggcttg gtgcatttcc
     2281 gcaacagaat agaaggctgg ctccagcacc attttttcat cctcctgctt gcggcgatgg
     2341 ttgtaccaca aggtagtcca accggcagcc tgaccgccga tgatatccgt ttcatacgta
     2401 tccccgatga aaagcagctc gccgggagtt gcgttcaaag actcctgaac atgcgcgaag
     2461 gcttcggcat agggttttgg gcaaccgata ttttcggaaa tgaagatgcg tgctggcggc
     2521 acccatcgct ccagattcaa ggcgttgaat tttttcatct ggtgtttttc aggaccgttt
     2581 gtgaagacgg caattggtat atttctattt tccagttcat tcaaaagccg ctcgataccg
     2641 ggactcaaca caatctcccc ttgccttttt tgataggatt tctggaagtg catcgcttct
     2701 tcggcggtga gcggaagacc caagtcggcg aatgtatgct gtgtgcgcat gatgtgtgac
     2761 tcggctagcg tcagctcccc gctggagaat aggtcgaagg cgttgtcaca atgctcgcgg
     2821 aaaagagcga agctggcttc gttactgtag ttgttcctaa agctgtaatc tgccaatgtt
     2881 tggaagaacg gccttgatgt gtcgtatagg gtatcatcca aatcgaatag aatagtaaac
     2941 attattgaaa cagttcctcc cttttgttat taaagtcagt atagcatcca aaatcggtat
     3001 ttaaaatgaa ataaaacaag gacagccagt gcgtgaaggg tactggctgg ctgagcaaag
     3061 ctctttttgt tgcaaattaa aattacgtac agtataatag catacaataa tgtcatacga
     3121 tcatacaata atggaagagg ctgcggacat caagagtaaa atgcccattt gaaaggggtg
     3181 ttcgccgaaa tacagcattg gctgtgttgg ggtcaggatt aagaatcctg gcactgtcag
     3241 aacgatttgc ccaaaatcgt ttgtggagta ctttcggtat tgcgggcaga aggaagctgg
     3301 attatagggc cttttgcttt atagtctatt tttttgcaca aaaacggact gaattatcgt
     3361 tgttatgacg ctctatgggt agaaaggtgg acaaaaacgt agttggacaa gagggttgtt
     3421 taattttttt gagcatgtaa ataggactat aaacattagg aggaactaga gatgaaagta
     3481 aaaacatgga aaacaacaat ggcggctttg atgggtttgt ttattttagc gggttgcggt
     3541 aacggaacta ctgaggattc atcggcagca gatggttcgg ctgaatcgga tgcattcgta
     3601 gtcggaatgg aagcgggata tccgcctttt aactggacac aaaatgatga cagcaatggc
     3661 gcagtgaaaa ttgatggcgc tgacggttac gcaaatggct atgatgttca gatggcccaa
     3721 aaagtggcgg
//
LOCUS       DAOMUJ010000140         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7013_length_3715_cov_2.065027, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000140 DAOMUJ010000000
VERSION     DAOMUJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7013_length_3715_cov_2.065027"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLT73_04670"
     CDS             <1..725
                     /locus_tag="PLT73_04670"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM19705.1"
                     /translation="VVRELIDPERPGDFNQALMDLGSSICSPLNPQPEISPIKAFNAA
                     YKNGTMHLYPVKSKAKRPVPMNLQGIILQNKEKQFLLEKRPSSGLLADFWTFPLIQLD
                     LDAIPGTVAAEPESAASIQTELLVAEEFQKLTETANKSDKKLPSFNQIEAKLSKVVAE
                     KYGLKPVWLKAETGSVNHVFSHIKWHITGYYGRVLTKEDSRLPKQCRWVSENDFADYA
                     FPVPQQKMWQQFKQQTRKEFGL"
     gene            849..1394
                     /locus_tag="PLT73_04675"
     CDS             849..1394
                     /locus_tag="PLT73_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HRM19706.1"
                     /translation="MHNPREGEFITIKSYKHDGSLHRTWRDTMVLKTSDQSIIGCNDH
                     TLVTEADGRRWVTREPALLYYHKHYWFNIVTMIRQKGVSYYCNLASPYVIDQEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYELHRNRMNYPKEIDHILKENVKILVSWINEEKGPFS
                     KEYVDLWYERYCQLSHNQQNS"
     gene            1569..2702
                     /locus_tag="PLT73_04680"
     CDS             1569..2702
                     /locus_tag="PLT73_04680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM19707.1"
                     /translation="MKKKEIVLVSIMGILTIAIVLFGYKYAAAKKEALLNEGEKQTET
                     VAETASSQESIVAESDTSEAYAQFLAAFAENRNSAPVIDYLQYVHHHTKEVNVVFFGD
                     VAADAAWAKSAIAGIQTDFPLENLSTSFFSYPSDSSSVYLSSQYVQEMVASNPDVIFY
                     TIPTKTDQVVDISLVDSTQNIYKIYDEIRAALPDTLIVLVTPPPAESKMADWNSRSLD
                     YRNYTNNLVEENAAFTVPLYDLHADFLAELTNRSETLANYMDAAGLELNETGQQLYGE
                     LFANSLRTKMVDTTAGLYVEGEKPAYTPIAPSLVVPVAPVTVTEEPVIEEDVYEAPVY
                     QVPTYVAPTNGYVEPVVEPTVPPEEPSDPSSDQVIDGAEAVAE"
     assembly_gap    2731..2740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3245..>3715
                     /locus_tag="PLT73_04685"
     CDS             3245..>3715
                     /locus_tag="PLT73_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19708.1"
                     /translation="MYIQVDERKRTDVLSTTYYQDNSDNLDDRLEQFVQSPVFDKKYS
                     FDGELGALYTPQRTILRLWAPTALSVEVIVYESLYRKEKKRHTMGKGGRGTHELIMIG
                     DYAGTAYKYAITFPDGKVVETVDPYSYATTANGERSVIMDAVNTNPKRWGARLEP"
BASE COUNT         1108 a          777 c          888 g          932 t
ORIGIN      
        1 cggtcgtgcg ggaactgatc gacccggagc gaccaggcga cttcaatcaa gcgttgatgg
       61 atctgggctc gagcatctgt tcgccgctga atccgcagcc tgaaatcagc ccgatcaagg
      121 ccttcaatgc cgcctacaaa aatggaacga tgcatcttta cccggtcaaa agcaaagcga
      181 aaaggccggt accgatgaat ctgcagggta tcatcctgca aaacaaggaa aagcagttcc
      241 ttttggagaa gcgtccgagc agcgggctac tggcggattt ttggacgttt ccgttgatcc
      301 aactggatct cgatgcgata cccggaacag tggctgcgga accagaatcg gcggccagca
      361 tccagacgga actgctggtt gcggaggaat tccagaagtt gacagaaact gcaaacaaat
      421 ccgacaaaaa gctgccctcc ttcaaccaga tcgaagcgaa gctttccaaa gttgtggctg
      481 agaaatacgg cttgaagccg gtctggttga aggcggaaac gggatctgtc aatcatgttt
      541 tcagtcatat aaaatggcac atcaccggct attacggacg cgtattaacg aaagaggata
      601 gccgtttacc caaacaatgc cgttgggtca gcgaaaatga ttttgccgat tatgcattcc
      661 cggtgccgca gcaaaaaatg tggcaacaat tcaagcaaca gacccgaaaa gaatttggct
      721 tataatggct aaaatttcgc aatccatttc cattctatga tatactgttc aatgaactga
      781 aagttgagag ttccgccgaa atgatttccg gcgagggctc aaatagttga agaaagttgg
      841 gcaccgtgat gcataatcct cgggagggag aattcatcac cataaagagt tataagcatg
      901 acggcagcct gcatcgaacg tggcgtgaca ccatggtatt aaaaactagt gaccaatcca
      961 taatcggctg taatgatcac accttagtaa cggaagcaga tgggcgtcgc tgggtcacca
     1021 gagagcctgc attgttgtat taccataagc attattggtt caacatcgtc accatgatcc
     1081 gccaaaaggg tgtctcctac tattgcaatc tggcatcgcc ttatgtgatc gaccaggaag
     1141 cattgaagta catcgattat gatttggaca ttaaagtttt tcctgatggt gaaaaacgtt
     1201 tgcttgatgt agatgagtat gaattgcatc gcaatcgcat gaattaccca aaagagatcg
     1261 accatatcct gaaggagaac gtcaagattt tggtgagctg gatcaacgaa gaaaaaggtc
     1321 cattctccaa ggaatacgtc gacttatggt atgagcggta ctgtcagctg tcgcataatc
     1381 agcaaaactc gtgatgaatc caaaataata aaaaaccagt cgagcgtaat gatcggctgg
     1441 ttttttatcg gaaatgtgcg ctaactcaaa aaaataccgt gcattaatac aactgttata
     1501 ttatgataaa tatatctgta ataaagagtc aatatttgaa atatcattaa aatattggag
     1561 gagtcagaat gaaaaaaaag gaaatagtgc ttgtttccat catgggtatc cttacgattg
     1621 ccattgttct ttttggatat aaatatgctg ctgctaaaaa agaggcatta ttgaatgaag
     1681 gtgagaaaca aacggaaact gttgcggaaa cagcttcttc ccaggagtct atagtcgcag
     1741 agagtgacac atccgaagct tatgcgcagt tcttggccgc gtttgcggag aaccgaaaca
     1801 gcgcgccggt catcgattat ctgcaatatg ttcatcatca tacgaaggaa gtcaatgtcg
     1861 tctttttcgg tgacgtggcg gcagatgcag cttgggccaa gtccgccatt gccggtattc
     1921 aaacagattt tccgctagaa aacctgtcga cttctttctt cagctaccct tctgatagca
     1981 gctcggttta tctgagtagc cagtatgttc aggaaatggt cgccagcaat ccggatgtca
     2041 ttttttacac gatcccgacg aaaaccgatc aagtggttga tatcagtttg gtggattcga
     2101 cgcaaaacat ctacaagatt tatgatgaaa tcagggctgc tttgcctgat acgctgatcg
     2161 tcttggtgac gccacctccg gcagagtcca aaatggctga ttggaattca cgttccttgg
     2221 attatcggaa ctataccaat aatctggtgg aagaaaatgc agctttcact gttccgttgt
     2281 atgacctgca tgcagatttc ttagctgagt tgacgaaccg cagcgaaacg ttggccaact
     2341 atatggatgc agccggtttg gaattgaacg aaaccggaca gcaactttat ggagaactct
     2401 ttgccaacag cctacgaaca aaaatggtcg atactacggc cggtctgtat gttgaaggtg
     2461 aaaaacctgc ctacacacct attgctccat cattggtcgt tccggtggcg ccagtcactg
     2521 taaccgagga gccagttatc gaagaggacg tgtacgaagc acctgtttat caagtgccga
     2581 cctacgttgc gccaactaat ggttatgtag agccagtcgt tgaaccaaca gtgcctccgg
     2641 aagagccgag tgatccttca tctgatcagg taatagatgg agcagaagct gtagctgagt
     2701 aaccttaaga tggcatcaac agtgtaggtt nnnnnnnnnn gagaacgccg atgaaactgg
     2761 aggaaatgca aatcaggaaa ctgaaagtgc tgcatctgac tgatcagacg atgtgttgag
     2821 aaaatccagc gaccgaatat cctcagtaaa tcatcaccaa taaatcacag aatataaagc
     2881 tcgcatgtat cggattgtcc ggtatatgcg agctttttta gtgatttttt cgtattatcg
     2941 cccacaataa agcggataag tcaacctgcc agcatctgga cttcacaaat catctgagtt
     3001 aggttaacac aaaaacatta ttgcttttac ttccggagat actcttcgga atattgatat
     3061 tataatattt ctgatgtata attatggcat gaacaaaaga tgtgagcgtt ttatcgaaag
     3121 gtgttctgtg taggtcgatg gttatgcatt tcacaaacat gcaagcaaat ttggagaccg
     3181 ccgcacaata gccagttgtt tgaacgcaat ttcatcagaa aagagatttt tcggaggtaa
     3241 tgtcatgtac atacaagttg atgaacggaa acggactgac gttctatcga ctacttatta
     3301 tcaagataac tcagataatt tagatgatcg actagagcaa tttgtgcagt cgcctgtttt
     3361 tgataaaaag tattcatttg atggagaatt gggggcactt tatacaccgc agcggactat
     3421 tctgcggttg tgggctccga ccgcgctcag tgtagaagta attgtttatg aaagtctgta
     3481 ccgcaaagag aagaaaagac acacgatggg gaaaggcgga agagggactc acgaactcat
     3541 tatgattggg gactatgcag ggactgccta caaatatgcg atcactttcc cggatggcaa
     3601 agtcgtcgaa acggtcgatc cctatagtta cgcgaccacc gcaaacggag aacgctccgt
     3661 cattatggat gcagtaaaca cgaatccaaa aagatgggga gcccgattgg agccg
//
LOCUS       DAOMUJ010000141         3713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7017_length_3713_cov_1.625478, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000141 DAOMUJ010000000
VERSION     DAOMUJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_3713_cov_1.625478"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLT73_04690"
     CDS             complement(<1..1215)
                     /locus_tag="PLT73_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM19709.1"
                     /translation="MLSRNERINQKKKEQLTNSFITTNKRLKRSVAVLSTSLFVTTVV
                     KPVELVLAAEKTALGTKDTQVYSTNPFLNQVIPSATIIAAKNDLYASVMMAQSILESG
                     WGTSALASTPNYNLFGIKGNYDGESVNMGTLEDSGGQNYYPINAEFRKYPSYAESLQD
                     YANLLANGTSWNPNYYAGAWKSNAASYQDATAYLTGRYATDTAYSAKLNRIIAQYGLD
                     QYDNYLLVDDEEVENPDAGNVDFETPTDSPDLPNVDLPENSDEDTGEQEKPSEPEEDS
                     EEVDTPDAPVQAGEAFHVVQSGDTLYAIAKKYGISLVDLLTLNKLTSNMIYVGDRLVL
                     PDSVIVEGNNPDEDTDEEKAPTTPTSEYTVVVGDTLYKIAAANGLTVGELKSLNALTS
                     DVIVPGQTLLVKK"
     gene            1538..2530
                     /locus_tag="PLT73_04695"
     CDS             1538..2530
                     /locus_tag="PLT73_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771012.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRM19710.1"
                     /translation="MTNQFLYSDTNKRYHTWNYHLQQHFGEKIFKVAIDGGFDCPNRD
                     GTVARGGCTFCTVSGSGDFAQDRVDPLPVQLRKGIDMMHKKWPNVKSYIAYFQNFTNT
                     HAPVDILRHRYEQVVNEDGVVGIMIATRPDCLPPETIAYLAELNKRYYIWVELGLQTI
                     HEETSKIINRAHSYDTYLKAVDELRAHNIPVCTHLINGLPGETHDMMMESVKRVILDS
                     DIQGIKIHLLHLMKNTKMLRDYHQGRLRLLEKDEYVGLVCDQLEVIPEKIVIHRITGD
                     APRDTLVGPMWSLKKWEVLNAIDHELERRNTYQGIHNIRNKGEEHVIVRSGLQS"
     gene            2499..3080
                     /locus_tag="PLT73_04700"
     CDS             2499..3080
                     /locus_tag="PLT73_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM19711.1"
                     /translation="MLLSAVDFSHSLLKETVCKGDLVIDATVGNGSDTVLLATLVGPT
                     GKVIGFDVQKQAVENTAQKLLLAGLTDQVTLLHQGHETLGDVLADNAKIGGAIFNLGY
                     LPGSDKTIITKKNTTISAIEALLPKLRIGSYILLVVYSGHPGGMEEKNALLDYCSSLN
                     QSLFKVLQYGFINQINHPPFLIAIEKKKTPYMK"
     gene            complement(3137..>3713)
                     /locus_tag="PLT73_04705"
     CDS             complement(3137..>3713)
                     /locus_tag="PLT73_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRM19712.1"
                     /translation="EQLKKDILEYVIHVVIDDSLLDADTKYFINPTGRFVIGGPKGDS
                     GLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAGLATKCEIQ
                     LAYAIGVAEPVSIAIDTFGTSEYPEATLIEAVRKNFNLTPAGIIQMLDLRRPIFKKTA
                     AYGHFGREDADFTWEKTDKTEALLSYVQANK"
BASE COUNT         1038 a          867 c          790 g         1018 t
ORIGIN      
        1 ttttttaacc aataatgttt gccccggaac gatgacatca ctcgtcaacg cattcaaact
       61 tttcaactca ccaaccgtca agccgtttgc tgcagctatt ttgtaaaggg tatcgccaac
      121 aactaccgta tattctgaag ttggagtggt tggtgctttt tcttcatccg tatcttcatc
      181 agggttgttt ccctcgacta taacgctgtc aggcaacacc aaacgatctc ccacatagat
      241 catattggaa gtcagtttat tcagcgtcaa cagatccact aatgaaattc catacttttt
      301 agcgattgca taaagcgtgt cgccggattg aacgacatgg aaagcctccc ccgcttgaac
      361 cggagcatcc ggtgtatcaa cttcttccga atcttcttca ggttcacttg gtttctcttg
      421 ttctccagta tcttcgtctg aattctcggg caggtcgaca ttcggcaggt caggactatc
      481 agtaggcgtt tcaaaatcga cattgcctgc atccgggttt tcgacttctt cgtcatctac
      541 aagtagatag ttatcatatt gatctaaacc gtattgtgca atgattctgt tcaacttggc
      601 actgtaggct gtatccgtag catagcgacc ggtcaaataa gctgttgcat cctggtaaga
      661 ggctgcattg cttttccaag caccggcata ataattcgga ttccaactcg ttccattcgc
      721 aagaagattt gcataatcct gcaaggattc ggcataggaa ggatattttc tgaattcagc
      781 attgattggg tagtaattct gcccgccgct atcctccagt gttcccatgt tgacagattc
      841 tccgtcatag ttccctttga tcccgaatag attatagttg ggagtagaag ctaaagcgct
      901 ggtaccccaa ccgctttcga ggatggattg agccatcatc acagaggcat aaagatcatt
      961 tttggcagcg attatggtcg cggaagggat gacctggttc aaaaaaggat tggtggaata
     1021 tacttgtgta tcctttgttc cgagcgcagt cttctctgct gccagaacca gttcgactgg
     1081 tttaacgaca gttgtcacga ataaggatgt actcaatacc gctacgctgc gtttcagtct
     1141 tttattcgtg gtgataaaag aattggtcag ttgttctttt ttcttttggt tgatgcgttc
     1201 gtttctggat aacaccaaaa tataccactc cttgtatcaa aaatactgcc tatatggcag
     1261 tgaaaacatc tggtttttta acaatgatgg aaagataatt taaatttctt ttaagaaata
     1321 atccaacata cctattatat gtttgtttat cacatttttg aatggctttc ataatcatat
     1381 tctatattta ataattgaaa taaatctgta acaaaccatt ccacgaacaa tatgtaaccc
     1441 ctttccccat tcaaattctt ctgaaatccg gaaaagctat caaatagact acagacagac
     1501 caaaaatcat gataaaatgg atactgaggt gaaagtattg acaaatcaat ttttatacag
     1561 tgacacgaat aaacgttacc atacatggaa ttatcatctg caacaacatt tcggcgaaaa
     1621 gattttcaaa gtagccatcg atggcggttt tgattgccca aaccgcgacg gcactgtcgc
     1681 acgcggcggc tgcaccttct gcaccgtttc cggatccggc gattttgccc aggacagggt
     1741 cgatccgctg ccagtccaat tgcgcaaagg cattgatatg atgcacaaaa aatggccgaa
     1801 tgttaaaagc tacatcgcct atttccagaa cttcaccaat acgcacgcac cggttgacat
     1861 tctgcgccac cgctatgaac aggtcgtaaa cgaggacggc gtggtcggca tcatgattgc
     1921 aacgcgcccg gactgcctgc cgccggaaac aatcgcgtac ttagctgaac tgaacaagcg
     1981 ctattatata tgggtggaat tgggacttca gaccatccat gaggagacaa gcaaaatcat
     2041 caaccgcgcg cattcctatg atacctacct gaaggcggtc gatgaacttc gtgcgcacaa
     2101 tatcccggtg tgtacccatc tcatcaacgg tcttccggga gaaactcatg acatgatgat
     2161 ggagagcgtc aaacgtgtta tcctggattc ggacattcaa ggcatcaaaa tccatttact
     2221 gcatttgatg aaaaacacta aaatgcttcg ggactaccat caaggacggc tacgtttgtt
     2281 agagaaagac gaatatgttg ggcttgtctg cgatcagctc gaagtcattc cggaaaaaat
     2341 cgtcatccac cggattacgg gagatgctcc gcgtgacacg cttgtcggcc cgatgtggag
     2401 cctcaaaaaa tgggaagtgc tgaacgcaat cgaccatgag ctggagcgcc gcaataccta
     2461 tcaaggaatc cacaatatca gaaataaggg tgaggaacat gttattgtcc gcagtggact
     2521 tcagtcatag tttgctgaag gaaacagtct gcaaaggaga tttagtcatt gatgccactg
     2581 tcggaaacgg cagcgatacc gtccttttgg ctacactggt cggtccgaca ggcaaagtga
     2641 tcggttttga cgtccagaaa caagccgtcg aaaatactgc acaaaaacta ttgttggcag
     2701 gacttacgga tcaggttacc cttcttcatc aagggcatga aacccttggg gatgtgctgg
     2761 ccgacaatgc aaaaatcgga ggtgccatct tcaacttagg atacctacca ggatccgata
     2821 aaacgatcat caccaaaaaa aatacgacca tcagtgccat cgaagccttg ctcccgaaat
     2881 tgcggatcgg cagttacatt ctcttagtcg tgtacagtgg gcatcctggc ggcatggaag
     2941 agaagaatgc cctgcttgat tactgcagta gcttgaatca atccttattc aaggtactgc
     3001 agtacggctt catcaatcag atcaatcatc cgccgttctt gattgccatc gaaaaaaaga
     3061 aaactcctta catgaaatag aaaaaaggaa agcgttccgg caaatgaccg caacgctttc
     3121 ctttttttgc ttgcttttat ttgttcgctt gaacatatga aagcaaagct tctgttttgt
     3181 ctgtcttttc ccaagtgaag tcagcatctt cacggccaaa atgtccgtag gctgccgtct
     3241 ttttgaagat aggacgacgt agatccaaca tttggatgat gccagctggc gtcaagttga
     3301 agtttttgcg cactgcctct atcaaagtag cttcgggata ttcgcttgtg ccgaatgtat
     3361 caatcgcaat cgatactggt tcagcaactc cgatcgcata ggccaattgg atttcacatt
     3421 ttgtcgccaa tccagccgca acaatgttct tcgcaatgta acgggccgca tagcttgcag
     3481 aacgatcaac ttttgtagca tccttacctg agaaagcacc gccaccatga cgcgcatacc
     3541 caccataagt atcaacgatg atttttctcc cagtcagacc ggaatcgcct ttaggtcctc
     3601 caatgacaaa gcgaccagta ggattgatga agtatttagt atcagcatcc aacaatgaat
     3661 cgtcgatgac cacatggatg acatactcca aaatatcttt tttcaattgt tcc
//
LOCUS       DAOMUJ010000142         3703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7041_length_3703_cov_2.348958, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000142 DAOMUJ010000000
VERSION     DAOMUJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_3703_cov_2.348958"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLT73_04710"
     CDS             complement(<1..502)
                     /locus_tag="PLT73_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRM19713.1"
                     /translation="MMESDSKPNLAHADYYFNRELSWLDFNKRVIEEAQDPQNPLLEQ
                     LNFLSIASSNLDEFYMVRVAGLQDQFKLGYHITDSKTDRTPVEQLSAISKKNSENIAL
                     QYKSYYELLNQLAEKNIRIKKISALSKSEFSIIEETFHEQIFPALTPLGIDAYRPFPN
                     LNNKLIH"
     gene            complement(554..2092)
                     /locus_tag="PLT73_04715"
     CDS             complement(554..2092)
                     /locus_tag="PLT73_04715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HRM19714.1"
                     /translation="MLLERIGLIDIGSNTIRLVIFEIDEHFTMFQVQNIKTPARLFQY
                     LDKKKKMSQAGIDVLIKVLKSFKSVADQYQVSVLMPVATAAIRQSTNRDDIIKQVRKA
                     TKIEMMLLPEEKEAFYGNYAVLHTTQFLNGVTIDIGGGSTEVTYYENKELQQYHSFPF
                     GVVTLKQMFFDGKEHNDPKAIKKMQEFVKDQFKSIKWLSKKQVPIVAMGGSARSIANV
                     HQRKIDYRLAGVHGYRMYKNDLQMTLDLFQSLSIPQLQNLDGLSRDRTDLIIPANVVF
                     NTLFDEVKAPVFLFSNKGLREGIVMELLNDKYENKAFSVYNIAAQSVSRLAASYHTLA
                     HVAQQRVKLVDMLYEELCRLGIFDKNPEMEKLLHFGSYLYYLGQFVETGASSQHTFYI
                     ISNSDLDGISHKERVSIALLASFKNRSLFNQYLTGFTDWYTVTLIDQLQSFGGIIKFA
                     DALNDSHMEVIRDIKIIKIKNGYDLVLSYKGDILAEEYRAERQRKHIERLLSGKLNII
                     FTEC"
     gene            2374..3537
                     /locus_tag="PLT73_04720"
     CDS             2374..3537
                     /locus_tag="PLT73_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRM19715.1"
                     /translation="MNHKKRMGKLMLAGLAASLALSITPVQGSTLDTLTQVEKQTEAR
                     IASVEVTIGETLASINLKQMEIDDLEAQIVAAEESQSETKTAIEAQKEVITSRKEQLK
                     TRLVALQTSDAMTNQILMLLDSENFADFISRAYVVGQLQAADNEQIESAISEEEKLMA
                     LEEQLADEIETVKVAEGRLKSESSALAEELSTLKDILSENQIVLSQVQTEYATEADRL
                     AAEAAQAKADAEAAQAAANAAEEHVTVAESTAPSLQEGTVATQEPESQITDSSVAAVE
                     TAPVVTAPTVTEPPASQGKTVTVSATAYSRHEAGLSNFTATGIDLSINPMVIAVDPSV
                     IPLGSIVSVPGYGIAIAGDTGGAIVGNKIDLHMEDLNAALAFGRQTLTITILQ"
BASE COUNT         1016 a          822 c          861 g         1004 t
ORIGIN      
        1 tatggatcag tttattgttc aagttcggga aaggacggta agcatcgatc cctaatggtg
       61 tcaacgcggg aaaaatttgt tcatggaaag tttcttcaat gattgaaaat tcactttttg
      121 aaagagcgct gatttttttg atgcgaatat ttttttcggc caattggttc aaaagttcat
      181 aataggactt gtattgcagc gcgatatttt cgctattttt cttggagatg gcagacaact
      241 gttcaacggg ggtcctatcg gttttgctgt ccgtgatatg gtagccgagt ttgaactgat
      301 cctgcaaacc ggctacccga accatataga actcgtcaag attggaactt gctatggata
      361 aaaagttcag ctgttcaagc aaaggattct gaggatcttg cgcctcctca atgacgcgtt
      421 tgttgaagtc caaccaactc agttctcgat tgaaataata atccgcatga gcaagattgg
      481 gtttactatc tgattccatc atacgacaca tccattcatt cgatttataa ggtttattta
      541 tagcataatt tcgttaacat tctgtaaaaa taatgttaag tttaccgctc agcagacgct
      601 cgatatgttt gcgttgacgt tctgcgcgat attcttccgc aaggatgtca cctttataag
      661 aaagtaccag atcgtaccca ttttttatct taatgatttt gatgtcccgg ataacttcca
      721 tatgagaatc gttaagggcg tcagcgaatt tgatgatgcc tccgaatgac tgcagctgat
      781 cgattagggt cactgtgtac cagtccgtaa atccggttaa gtattggttg aagagtgaac
      841 ggtttttgaa gctggccaat aaagcgatgc ttacccgttc cttatggctg atgccatcaa
      901 ggtcgctatt ggagatgatg tagaaggtat gctgcgaact ggcgccggtt tcgacgaatt
      961 ggccaaggta ataaaggtaa cttccgaaat gcaagagttt ctccatttcc ggatttttgt
     1021 caaagatacc cagtcgacac aactcttcat acagcatatc gaccagcttc accctttgct
     1081 gcgcaacatg cgccaacgtg tggtagctgg cggcaagcct gctgaccgat tgggcagcga
     1141 tgttgtaaac actgaaggct ttattctcat atttatcgtt cagtaattcc atcacgatac
     1201 cttcacgcaa ccctttattg ctgaacaaaa acacgggggc tttcacttca tcgaagaggg
     1261 tattgaatac cacattagcc ggtatgatca gatcggtccg gtcccggcta agcccatcca
     1321 gattctgcaa ctgcgggatg ctcaatgact ggaacaaatc caaggtcatt tggagatcat
     1381 ttttgtacat acggtaacca tgcacgcccg ctaaccgata atcgatttta cgttggtgga
     1441 cattcgcgat gcttcgggca gaaccgccca ttgcaacgat gggcacttgc ttcttgctca
     1501 gccacttgat cgatttgaac tggtccttca caaattcttg catcttcttg atcgcctttg
     1561 ggtcattgtg ctctttgccg tcaaagaaca tctgttttaa ggtcacgacc ccaaaaggaa
     1621 aactgtggta ttgctgaagt tctttatttt catagtaggt gacttccgta ctgccaccac
     1681 cgatgtcgat cgtgacgccg tttaggaatt gggtcgtatg cagaacagca taattcccat
     1741 aaaaagcttc cttctcttcc ggcaacagca tcatttcgat tttggtagct ttgcgtactt
     1801 gtttgatgat atcgtcgcgg ttggtggact gacggatagc agccgtcgct accggcatca
     1861 aaacgctgac ttgatactga tcagcaacag atttgaaact tttcagaact ttgatgagta
     1921 cgtcgatacc tgcttgggac atctttttct tcttgtccaa atactgaaaa agacgtgcag
     1981 gggtcttgat gttctggact tgaaacattg taaagtgttc atcgatttca aagatgacca
     2041 aacggatggt attggatcca atatcgatca aacctattct ctctagtagc atgagcgtct
     2101 ccttctgaat cccgggtctt ccaagattcc tcactttcca ttttactgga atttatcgtt
     2161 tttgtcatag ttttctctta gtgagctagt gaaacaggag agaaatgtta cgggaaagaa
     2221 tcttatgtta cgaatggatt acgaaaaggt tcctaataag gcaatgagag caccaggtga
     2281 atcatttgat acgaaacagt aaaatgaatg caaccctccc ataagagaat ttcgatataa
     2341 tgaaggagca tttcaaaaga gaggcgtgag attttgaatc acaaaaagcg tatggggaaa
     2401 ctgatgttgg ccggcttggc agcaagtctg gccctttcca ttacacctgt tcaaggttcc
     2461 accttagata ctttaactca agtagaaaaa cagacagaag ctcgaatcgc ttcagtagaa
     2521 gttacaatcg gcgaaacact ggcctcgatc aacctgaagc aaatggagat tgatgacctg
     2581 gaggcgcaga ttgttgctgc agaagaaagt caaagcgaga caaaaactgc aatcgaagct
     2641 caaaaagaag taatcacttc acgcaaagaa caactgaaaa cacggctcgt tgctttgcag
     2701 acttctgatg caatgacgaa tcagattttg atgttgctgg attccgaaaa ttttgccgat
     2761 ttcatcagca gagcctatgt cgtcggtcaa ttgcaggctg ccgataatga acagatcgaa
     2821 tctgccattt cggaagagga gaaactgatg gcattggaag aacagttggc tgatgaaatc
     2881 gaaacagtca aggttgctga aggccgtctg aaatctgaat cctctgcgct ggcggaagaa
     2941 ttaagcacat tgaaggacat cctgtccgaa aaccaaatag tgctgtcgca agtgcaaact
     3001 gagtatgcaa ctgaagcaga ccgtttggct gccgaagctg ctcaagccaa agccgatgcc
     3061 gaagcggccc aagctgcagc gaacgcggcg gaggaacacg ttacggttgc tgaatctaca
     3121 gcgccttccc tgcaagaggg aacagttgcc acacaagaac ccgaaagcca aattaccgac
     3181 agttcggttg ccgctgttga aacagcgccg gtcgtaaccg cgccgactgt gacagagcca
     3241 cctgcttccc aaggcaaaac agtgactgtt tcagcaacag cctattcgcg ccacgaagct
     3301 ggattatcca actttaccgc cacaggcatc gacctttcga tcaatccgat ggtcatcgcg
     3361 gtggatcctt ccgtgattcc attgggaagt atcgtcagcg tgccgggcta tggcattgcg
     3421 atcgccggag acaccggtgg agcgatcgtc ggcaacaaaa tcgacttgca catggaagac
     3481 ttgaacgctg cgcttgcctt cggcagacag acgttgacga tcaccatcct gcaataagaa
     3541 aagctgaacg ggttcgttca gccctgagtt atgttaaaaa aagaatcgga tccttttcaa
     3601 ttatgcgaaa gggtccgatt ctttttgttt ttgagtgggc agcgcgtcac cagcctgtca
     3661 ataatttccc gaactccgat aaagcgatgc ttggccgaag gtg
//
LOCUS       DAOMUJ010000143         3691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7071_length_3691_cov_2.358636, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000143 DAOMUJ010000000
VERSION     DAOMUJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_3691_cov_2.358636"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLT73_04725"
     CDS             complement(<1..241)
                     /locus_tag="PLT73_04725"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRM19716.1"
                     /translation="MKIESKFQKKKPVLSFEIFPPKRDKAIQNIDETLAILSELNPDF
                     ISVTFGAGGSHTDNQTVELAKRIKQQYGIDPLVHLT"
     gene            complement(259..2541)
                     /gene="metE"
                     /locus_tag="PLT73_04730"
     CDS             complement(259..2541)
                     /gene="metE"
                     /locus_tag="PLT73_04730"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM19717.1"
                     /translation="MNSSVIGFPRVGKLRELKFASEKYFKQEISTEELEQVAKGLREM
                     HWNLQQAAGISHIPSNDFSYYDNVLDLTVLLNALPENYRQLGLSERDTYFAAARGYQG
                     VAGDAKALAMKKWFNTNYHYLVPELSDNTDIKLVGDKPFVEYEEAKAIGVLTKPVLIG
                     GFTFLKLAKYKGSKTINDFADQVADAYIAILDRFNEQGVEWVQFDEPSLVTDLTQEDV
                     ALFTALYEKVLAHKGNVKVLLQTYFGDIRDSYEEVVGLDFDGIGLDFIEGKQTVELLE
                     RYGFPADKTLFAGVLNGKNIWKSDYKKVITLVNGLKKYSNDIVISTSCSLLHVPYTLE
                     NETSLPEEVSQYFAFAKERLQEIKELTELIGTSGSGYEEQAAYLANQKVFSADRVYED
                     KHVQASVASLTERDFVRNVPRQKRRAIQKEKLQLGLLPTTTIGSFPQTREVKQNRSKY
                     RKGAISKAEYDENIKGFIKECIDLQEELGLDVLVHGESERNDMVEFFGENLAGYVFTE
                     KAWVQSYGTRCVKPPIIFGDIRRENPITVFYSEYAQSLSDKPVKGMLTGPVTILNWSF
                     PREDISLREMAFQIGLAIREEVLDLEEAGIKIIQIDEAALKEKLPIRREEWASEYLDW
                     AIPAFRLCHSGVRPETQVHTHMCYSEFEEIVKDIDNMDADVISFEASRSKLTIIDALK
                     ANQFETEVGPGVYDIHSPRVPSVEEMVAVLKNALTKIDEENLWINPDCGLKTRGIKET
                     RESLANLVAAAKIIKDAVLV"
     assembly_gap    2935..2944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3161..>3691)
                     /locus_tag="PLT73_04735"
     CDS             complement(3161..>3691)
                     /locus_tag="PLT73_04735"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM19718.1"
                     /translation="GMDALTHAIESMVTPGAYAVTEVLASGAIELIREYLPKAVENGT
                     DLDARDKMVNAIFLGGMAFNNAGLGYVHSMAHQLGAVYHLPHGVCCAMLLPIVEAENA
                     KFAPERFRKVAKALGLAVTADVGIPTSLNELGIKEEEFDYDYLSKNAMIDACAPGNPF
                     TPTLEETIAMYKKLFQ"
BASE COUNT          817 a          969 c          834 g         1061 t
ORIGIN      
        1 aagtaagatg caccagcgga tcgatcccgt actgttgctt gatgcgtttg gccaattcaa
       61 cagtctgatt gtctgtgtgg cttccaccgg caccaaaagt gacgctgatg aaatcaggat
      121 tcagttcact caggatcgca agcgtttcat cgatgttctg gatcgcttta tcgcgcttcg
      181 gtggaaaaat ttcgaaggaa agcaccggtt ttttcttttg gaatttggat tcgattttca
      241 tcagccttca cctctttttt agaccaaaac tgcgtctttg atgattttcg ccgccgccac
      301 aagattggcc aggctttctc ttgtttcctt gatgcctctt gttttcagtc cgcagtccgg
      361 attgatccat agattttcct cgtctatctt cgtcagcgcg ttcttcaata ccgctaccat
      421 ctcttcgaca ctcggcacgc gcggcgagtg gatgtcgtaa acacccggtc cgacttccgt
      481 ttcgaactgg ttcgccttca aggcatcgat gatcgtcagt ttggaacggg aggcttcaaa
      541 agaaatgacg tccgcatcca tgttatcgat atcctttacg atttcctcga attcgctgta
      601 gcacatatgc gtatgcacct gcgtctccgg tctcacgcca ctgtggcaaa ggcggaatgc
      661 cggaatcgcc caatcaaggt actcgctcgc ccattcttct ctgcggatcg gcaacttctc
      721 cttcagagcc gcttcatcga tctggatgat tttgatgccc gcttcctcca aatcgagaac
      781 ctcttcgcgg atcgccagac cgatctggaa agccatctcc ctcagcgaaa tgtcctcacg
      841 cgggaacgac caattcaaga ttgtcaccgg accagtgagc atccctttga ccggtttgtc
      901 ggaaagactc tgcgcatatt ccgaatagaa cacggtgatc gggttttcgc gtcggatgtc
      961 cccgaaaatg atcggcggct tgacgcatct tgttccgtag gactgtaccc aagctttttc
     1021 ggtaaacacg tatccagcca ggttttcgcc gaagaattca accatgtcgt tgcgctcgga
     1081 ttcgccgtgg accagcacat ccagccccag ttcctcctgc agatcgatgc attctttgat
     1141 gaagcctttg atgttttcat cgtattcggc ttttgagatc gcgcctttgc ggtacttgct
     1201 gcggttctgc ttgacttccc tcgtctgcgg gaaggaaccg atcgtcgttg tcggcaacag
     1261 acccagttgc agtttctctt tttggatcgc tctccgtttt tgtctcggaa cgtttctgac
     1321 aaaatctctt tccgtcaagc tcgctacgga tgcttgcacg tgcttgtctt cgtagacccg
     1381 gtccgctgaa aagacttttt gattcgcaag ataagctgcc tgctcttcat atccggatcc
     1441 ggatgtgccg atcaactccg tcagctcttt gatttcctgc agcctttcct tcgcgaaagc
     1501 gaaatactgg gaaacttctt ccggcaagct cgtttcgttt tccagcgtgt agggaacatg
     1561 cagcaacgaa caggaagtgc tgatgacgat gtcgttgctg tatttcttca acccattgac
     1621 caacgtgatg acctttttgt agtcgctctt ccagatgttt ttcccattca ggacaccggc
     1681 aaacaatgtt ttatcagccg gaaagccata tctttccaaa agttccacag tctgtttgcc
     1741 ttcgatgaaa tcaaggccga tgccgtcgaa atcgagcccg accacttctt catagctgtc
     1801 cctgatatcg ccgaagtatg tttgcagcaa tacttttacg ttacccttat gcgccaagac
     1861 tttttcgtaa agggccgtga acagcgctac gtcttcttgc gtcagatcgg tgaccaggct
     1921 cggttcatcg aactgcaccc actccacacc ctgttcgttg aaccgatcga ggattgcgat
     1981 ataggcatct gccacttggt cggcaaaatc atttattgtt ttcgagcctt tgtatttggc
     2041 cagcttcagg aaagtgaatc cgccgatgag gaccggtttc gtcagcacac cgattgcttt
     2101 ggcttcttcg tactcgacaa aaggcttatc gcccaccaat ttaatgtcgg tattgtcgga
     2161 cagttctgga accaaataat gatagttggt gttgaaccat ttcttcatcg ccaaggcttt
     2221 ggcgtcgccc gctacgccct ggtatccgcg cgcagccgca aaataagtgt cgcgctcgct
     2281 caaaccgagc tgtctgtagt tttcaggcaa cgcgttcaac aggaccgtca gatccagcac
     2341 gttatcgtaa tatgaaaaat cattcgaagg gatgtggcta atgccggcag cctgctgcag
     2401 attccagtgc atctcccgca aaccctttgc gacttgctcc aactcttctg ttgaaatttc
     2461 ctgcttgaaa tatttttcgg atgcgaactt caattcgcgc aatttaccga cccttggaaa
     2521 accgattact gaactgttca ttttgattcc tccatttcat tttttggttc ctgctgcaaa
     2581 actatcaatc atgcttttaa gtgttgtact gtttgctgat catcattcat cagaaggtgg
     2641 tttcagtatg ccacggaatc cagatatagg ataacactta ataaatatac gcagtcatag
     2701 ttttacacta taacattatg cggcctgatc ctagttgact ggagttggac cgggttttgt
     2761 cggctgtttt gagcatttca catcgcgaac tgtcgagaga ccaaacttct gtcggctgtt
     2821 ccaagcattc cacgtcgcag acagccgaga gatcagtctt ctgtcggctg tttcgaacat
     2881 ttctcgtcgc agacagccga gagatcagtc ttctgtcggc tgttccgagc attcnnnnnn
     2941 nnnnaaaaag ccgagagtcc gctattctgt cggctgtacg accttcatgg catcctcaac
     3001 agccgacact cagttctata gtcggctttt ggcaaaccaa taaagaagag ccacctcaaa
     3061 aatcgaggcg gctcttcttt attgtcgaca atcttgtttt ggacaaattc ggattatctg
     3121 catttctttg aaaacttagt aattttagct caggtttgga ttattggaac aattttttgt
     3181 acattgcaat tgtttcttcc aatgtcggag tgaatgggtt tcctggtgcg caggcatcga
     3241 tcatggcatt tttggacagg taatcgtaat cgaattcctc ttccttgatg cccagctcat
     3301 tcaatgaggt tggaatacca acgtcagctg tcactgccaa tcctaaagct ttcgctactt
     3361 tgcggaaacg ttcaggcgcg aatttggcat tttcggcttc cacgattggc aacagcattg
     3421 cgcagcaaac gccgtgcggc aagtggtaga cggcacccaa ttggtgagcc atcgaatgca
     3481 catagccaag gccggcattg ttgaaggcca ttccgcccaa gaagatggca ttgaccattt
     3541 tgtcgcgcgc atcgagatcc gtgccgtttt cgacagcttt cggcaagtat tcgcggatca
     3601 gttcgatcgc tccggaagcc aaaacttccg tcacagcgta agcgccaggc gttaccatcg
     3661 attcgatcgc atgcgtcaaa gcatccattc c
//
LOCUS       DAOMUJ010000144         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7089_length_3682_cov_2.213124, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000144 DAOMUJ010000000
VERSION     DAOMUJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7089_length_3682_cov_2.213124"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..383
                     /locus_tag="PLT73_04740"
     CDS             54..383
                     /locus_tag="PLT73_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HRM19719.1"
                     /translation="MKLIMAIIQDKDSAILSDELVEANVRATKLPSTGGFLRAGNTTF
                     MIGVEDERVDEVLRIIKTNCEAREQFVATPVSMDVPLDNAIAYPMEVHVGGATVFVLP
                     IDSFYRF"
     gene            398..>624
                     /locus_tag="PLT73_04745"
     CDS             398..>624
                     /locus_tag="PLT73_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRM19720.1"
                     /translation="MDGVTQLLMRQQPELLERFQRTIQEKRLVHAYLFEGARGTGKEE
                     LARWIAQTLFCDELQDGQPCGHCNHCLRIAAG"
     assembly_gap    625..634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <635..1397
                     /locus_tag="PLT73_04750"
     CDS             <635..1397
                     /locus_tag="PLT73_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRM19721.1"
                     /translation="EFPDVAEISPDGNTIKVNQVRELKAELSKSGMEGSRKVYLIYDA
                     EKMTVSASNSLLTFLEEPQNDTYLILMTTAKENILPTIRSRCQIVHFRVVNKQALGET
                     LEAQGIQPENAALLAAITNNQEAALLLNQEESFHESKKRTWAWFQLMTSKNPQAFVFV
                     QTDLMDGLKDKNDGHFFLDLLLFYYRDLLYTKYSNKQAIVNKNHGTEYERIAEKLHPQ
                     EITRHMENILKGKKHLEANVQLQGVLEEIALGNSL"
     gene            1423..2217
                     /locus_tag="PLT73_04755"
     CDS             1423..2217
                     /locus_tag="PLT73_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRM19722.1"
                     /translation="MKNVIGVRFMPVGPISYYEAGQTKYKMDEKLIINNGNAVDIGQV
                     VIVDKQCKNDESGIHAHKHIIRVATEKDLEQDEKNRAEAKEAFAVCKTKMKQLKLEMK
                     LIKAEYTFDRSRLTFHFSSEGRIDFRELVRELAAVFRTRIELHQIGVRDEAKILGGIG
                     PCGRTLCCSTFLGDFVPVSIKMAKDQGLSLNPAKISGLCGRLMCCLNYENDTYVSLRK
                     AMPDYGQEVMTPEGKGKVVELNVLSQVVKVRLFEHNKTVEFAGSEL"
     gene            2277..2627
                     /locus_tag="PLT73_04760"
     CDS             2277..2627
                     /locus_tag="PLT73_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HRM19723.1"
                     /translation="MDKRALYDQFHRVESRISSMGADVKEIQEKMDDLIEENANLQIE
                     NQHLRDRIDFLTKEKEDAQKQEPEMSKSRLNLQKLYEDGFHVCNVYYGSRRLNDEPCV
                     FCIDVIYGERDRKN"
     gene            2739..3491
                     /locus_tag="PLT73_04765"
     CDS             2739..3491
                     /locus_tag="PLT73_04765"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HRM19724.1"
                     /translation="MENVLKEGERLDVLKRENIQIIQSSAVFSFSLDAVLLADFAELP
                     RVKPAKIVDLCAGNGAVSFLLTGKTKNPIIGVELQDRLVDMARRTVQLNQLEEKVSFL
                     HADVNDVTRFIKPDSVDVITCNPPYFKLTESSFTNELDAFAIARHEIHLTLDQLMKQT
                     SHLLKMKGKAYFVHRPDRLMEILETMRNNRIAPKKLQFIYPKQNKEANMILIEGIKDG
                     KEDGFRVLPPLVVYNEEGEYSQKVKDVLYGEQ"
     gene            3478..>3682
                     /locus_tag="PLT73_04770"
     CDS             3478..>3682
                     /locus_tag="PLT73_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM19725.1"
                     /translation="MASSEHYFYVLLCADDSFYAGYTTDTLRREREHNKGIGSKYTKA
                     RRPVRMIYKEVFGSRTEATKAEAA"
BASE COUNT         1055 a          771 c         1000 g          846 t
ORIGIN      
        1 ctgaacgaac ccgttcagct tttcttatat attatagaaa gaggggattt gttatgaaat
       61 taattatggc tatcattcaa gataaagaca gcgctatcct gtccgacgag ttggtggaag
      121 cgaatgtccg cgctacgaaa ttgccttcta cgggcggttt tctgcgcgcc ggcaacacca
      181 ccttcatgat cggtgtagag gacgaacggg tcgatgaagt attgcggatc atcaaaacca
      241 actgcgaagc gcgcgagcaa tttgtagcga ccccagtcag catggatgtg ccgttggaca
      301 acgcaatcgc ctacccgatg gaagtgcacg taggcggagc gactgtgttt gttttgccga
      361 tagatagttt ttacagattc tgatgacgaa ggaaaggatg gatggtgtga cacagctttt
      421 gatgaggcaa caaccggaac tgctcgaaag gttccaacgg acgattcagg aaaagcggct
      481 ggttcacgcc tacttgtttg aaggagcgcg cggaaccggc aaagaagaat tggcgcgttg
      541 gatagcccaa acgctctttt gcgacgagtt gcaggatggt cagccatgcg ggcactgcaa
      601 tcattgccta cggattgcgg caggnnnnnn nnnncgaatt tcccgatgtg gcggaaatca
      661 gtccggatgg caacaccatc aaagtcaatc aagtccgtga attgaaggca gagctttcaa
      721 aaagcgggat ggaaggcagc cgtaaggtct atctgattta tgacgccgaa aaaatgacgg
      781 tcagtgcttc gaacagtctg cttacgttct tggaggaacc gcagaacgat acgtacctga
      841 ttctgatgac tactgcaaaa gagaacatcc tgccgaccat ccgatcgcgc tgccaaatcg
      901 tccattttcg ggtcgtcaac aagcaagcgt taggggaaac tttggaggcg caaggcatac
      961 agcctgaaaa tgcggcgttg ttggcggcca tcaccaataa ccaggaagca gctctgttgc
     1021 tgaatcagga agagagtttc catgaatcca agaagcggac gtgggcttgg ttccaactga
     1081 tgacttctaa aaatccccaa gcatttgtgt ttgtgcagac cgacttgatg gatggtctga
     1141 aggataaaaa tgatggccac ttctttttgg acttgctatt attctattac cgtgatttac
     1201 tttataccaa gtacagcaat aagcaagcta tcgtcaacaa aaatcacgga acagagtacg
     1261 agcgcattgc agaaaaactg catcctcagg aaatcacccg gcatatggaa aatatcctca
     1321 aaggaaaaaa acatttggaa gcaaatgtcc agctccaagg ggtactggaa gaaatcgcac
     1381 tcgggaatag cctatagata aagcttctgg aggagaaaga aaatgaaaaa cgtcattggt
     1441 gttcgtttta tgcctgtcgg gcccatctct tattatgaag caggccagac aaagtacaaa
     1501 atggacgaaa aattaatcat aaataatggg aatgctgtcg atataggtca agtcgtcatt
     1561 gttgataaac aatgcaaaaa tgatgagagt ggcatccatg cacataaaca tatcatccgc
     1621 gtcgcgaccg aaaaagatct tgagcaggat gaaaaaaatc gcgcagaagc gaaggaagct
     1681 tttgccgtat gcaagaccaa aatgaagcag ctgaaattgg agatgaagct gatcaaagcg
     1741 gaatacactt ttgatcgcag tcgtttgact ttccatttca gttcggaagg ccggatcgat
     1801 ttccgggaat tagtccgcga gttggcggct gttttccgca cgcggatcga attgcatcag
     1861 atcggtgtcc gcgatgaggc caaaattcta ggcggaatcg gaccttgcgg cagaacgctc
     1921 tgctgttcga cgttcttggg ggactttgtg ccggtttcga tcaagatggc gaaggatcag
     1981 gggttgtcct tgaatcctgc caaaatatcc ggtttgtgcg ggcgtttgat gtgctgcctg
     2041 aactatgaga acgatacgta tgtctctctc agaaaagcaa tgcctgatta tgggcaggaa
     2101 gtaatgacgc ctgaaggtaa ggggaaagtt gtggaactga atgtgctcag ccaagtcgtc
     2161 aaagtgcggc tgtttgagca taataaaacg gttgagtttg ccggttcaga attgtaaggt
     2221 ttaaggatct gcacgatagg attgaaaaat aagtatggaa aatgcgagtg gataaaatgg
     2281 ataaacgtgc cttgtatgac caatttcatc gggtggaaag ccgtatttca tcgatgggag
     2341 cagatgttaa agagattcaa gagaagatgg atgacctgat agaagagaac gcaaatcttc
     2401 aaatcgagaa ccagcatctg cgcgatcgga tcgatttcct tacgaaggaa aaagaagatg
     2461 cgcagaagca agagccagaa atgtcgaagt cccgtctgaa tttacagaag ctttacgagg
     2521 acggttttca tgtctgcaat gtttattatg gttcccgtcg tctgaatgat gagccctgtg
     2581 ttttctgtat cgatgtcatc tacggcgaaa gagatcggaa aaactgagaa gtgtcccgcg
     2641 ttgggggtac tgtcaataaa tgatggatga acaaatagac agaggcgggg acagaagtcc
     2701 tggcctctgt tttgaggata aggtaaaggg gtagaggaat ggaaaatgta ttaaaggaag
     2761 gcgaacgact tgatgtgctg aagagggaaa atatccagat cattcaaagt tcagccgtgt
     2821 tctctttttc gctggatgcg gtgctgttgg cggatttcgc tgaattgccg cgcgtgaagc
     2881 cggcaaagat agtcgatctt tgtgccggga acggggcagt ctcgttcctg ttgacaggaa
     2941 agaccaagaa tccgatcatc ggggtggaac tgcaggaccg gttagtggat atggcccgca
     3001 ggacagtcca gctgaatcag ctggaggaga aggtttcctt tctgcacgcg gatgtgaacg
     3061 acgtgacccg cttcatcaaa ccggattccg tcgatgtcat cacctgcaac ccgccctact
     3121 tcaagctgac cgaatccagt ttcacgaatg aattggatgc ttttgcgatc gcgcggcacg
     3181 aaatccattt gacgctggac caattgatga aacagacgag ccatctgctg aaaatgaaag
     3241 gcaaggctta tttcgtccat cgcccggatc gtctgatgga gatcctggaa acgatgcgca
     3301 acaaccggat tgcgcccaag aaactgcagt tcatttaccc gaaacagaac aaggaagcga
     3361 acatgatcct gatcgagggc atcaaggacg gtaaagaaga cggtttccgt gtgctgccgc
     3421 cgctcgttgt gtacaatgaa gaaggggaat acagccagaa ggtaaaggac gtcctctatg
     3481 gcgagcagtg agcactactt ttatgtgctg ctttgcgcag atgactcttt ttatgccggc
     3541 tacacgacgg acacgcttcg gcgcgaacgg gagcacaaca aaggcatcgg cagtaaatat
     3601 accaaggccc gccgccctgt ccgaatgatc tacaaagaag tcttcggttc cagaacggaa
     3661 gcgaccaaag cggaggcggc at
//
LOCUS       DAOMUJ010000145         3678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7100_length_3678_cov_2.393320, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000145 DAOMUJ010000000
VERSION     DAOMUJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_3678_cov_2.393320"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLT73_04775"
     CDS             complement(<1..450)
                     /locus_tag="PLT73_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRM19726.1"
                     /translation="MLYPFFDSTYILIIFGIAVSMIASGFVKRTFNTYNQVKSQNGYT
                     ATDAARFILEQSGIHDVRIERVRADLTDHYDSRAKVLRLSDTTANSTSVAAIGVAAHE
                     VGHAIQDQKNYIPLRLRAALVPAANFGQTLSMPMIMIGVFAGMNQTFL"
     gene            complement(686..1885)
                     /locus_tag="PLT73_04780"
     CDS             complement(686..1885)
                     /locus_tag="PLT73_04780"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HRM19727.1"
                     /translation="MKYDFETPVNRSNTGSEKWAAMYRSNPNVGKDVFPFSVADLDIK
                     TAPEIVEGLQEYIETAVMGYDLPTEDYYRAIINWMGNRHAWEIKKEWIICTPGIIAAF
                     YSAIRAYTDPGEGVLYMGPVYYPFMKSIKNTKRKLINNSLIRNGDRYEIDFEGLTEKA
                     KEPNTKLMLFSNPHNPVGRVWTKAELERVVDICAANDVVILADEIHHDLIMPGHVFTP
                     MASISESAADICITATAASKSFNIAGLRNSNVIISNPELHTAFKMDMEMTGIGGGTNV
                     IGLKATEIAYTAAEDWLEAFKALIWHNHETLKKFLAKELPEVTVFDLEGTYLQWMDFS
                     AFGLSHKQLEEIMEQEAQVFLDEGYIFGDEGRGYERISLGAPTKTLMEAMERLVTVMK
                     KYRTQNQ"
     gene            complement(2034..>2325)
                     /locus_tag="PLT73_04785"
     CDS             complement(2034..>2325)
                     /locus_tag="PLT73_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM19728.1"
                     /translation="VEMQQKGESIQVIDTRSAKDFEKSHVQDAIHVPLGELRARAGEL
                     SKDIPTVTYCNKGVTGNAAQNVLINLGFQEVYNLSGGNKNYQSYLIMLKRKN"
     assembly_gap    2326..2335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2336..>3678)
                     /locus_tag="PLT73_04790"
     CDS             complement(<2336..>3678)
                     /locus_tag="PLT73_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM19729.1"
                     /translation="VCGIPYKIGGEVDSVDRLTPRDAKWFKKRYDVSIFTEHRVTKVD
                     HEAKKIQVKDLKTGEVKEDAYDVLVFATGAAPLTPPPFDQAEYDNVFHVRNIQDLREI
                     ESYSEANKPKKALLIGAGFIGLEMTEQLVQKGWDVTIVQLENQVMPSMDADMAFRVEE
                     VLMANGVHLHLGDTVKTIEGKGTVKKVVTTYGATIETDIVILAAGVRPNTELAKEIGV
                     TIGAKGAVAVNSKMQTNIQDVYAVGDVAESFSAITGKPIYRPLGSTANKMGRIAGDVI
                     TGGNLEHRGVLGTGIFRVFDLHVGQTGMTEKEAKAADYSVEVLYNIKPDHAEYLGGKE
                     LIIKALADKATGRVLGAQVLGSQGVDKRIDVLATAITFKAKAEDLFHLDLAYAPPFAT
                     TKDPIHYTGMALDNALHGNPLMTPAQPCLLVMIPSPHRLDEHRAAPTARSRPRIR"
BASE COUNT          820 a          948 c          882 g         1018 t
ORIGIN      
        1 gaggaaggtc tgattcatgc ctgcaaatac gccgatcatg atcatcggca tggaaagtgt
       61 ctgaccgaaa ttagcggccg gaaccagagc ggcacgtaac cgcaacggaa tatagttttt
      121 ttggtcctgg attgcatgtc cgacttcatg ggcagcgacg ccgatggccg cgaccgaagt
      181 cgagttggct gtcgtatcgg aaagacgcaa aactttagcg cgcgagtcgt agtggtccgt
      241 cagatctgcg cggacgcgtt cgatccgcac atcatggatg cccgattgtt cgaggatgaa
      301 gcgcgctgca tccgtagcgg tgtatccgtt ttggcttttc acttggttat aggtattgaa
      361 ggtacgcttg acgaatcccg aagcaatcat ggaaaccgcg atgccgaaga tgatcaatat
      421 ataggtgcta tcgaagaatg gatacaacat gtatcatcaa cctttctttt atttattcta
      481 cagtatagca tccgattgtg aagaaaggat gaaggatgct taggttggga taattttata
      541 aaattcttta gatattattg tttgcaaaac cggattccgc ttggacggag taaatcaact
      601 gaagtgggag cagcgcgctg ctgtccatgg gtttccacca aaactctagg gctgcctcga
      661 aaaatcgaga cagccctcag tttttctatt gattttgtgt ccggtatttt ttcatgactg
      721 ttaccaagcg ctccatcgct tccatcaacg ttttcgtagg tgcccccaga ctgatccgct
      781 catatcctcg accctcgtcc ccgaagatgt agccttcatc caggaacact tgcgcttcct
      841 gctccatgat ttcttccaac tgcttgtgcg acaacccaaa ggcactgaaa tccatccatt
      901 gcaggtaggt gccttccaga tcgaagacag tcacttccgg cagttccttc gccaagaact
      961 tcttcagcgt ctcatggttg tgccagatca aagctttgaa cgcctccaac cagtcctcgg
     1021 ctgccgtgta ggcgatctct gtcgccttca agccgataac attggttcct ccgccaatgc
     1081 cggtcatttc catatccatc ttgaaagccg tgtggagctc cggattcgaa atgatgacat
     1141 tggaattgcg caggcccgcg atattgaagg acttgctggc ggcggttgcc gtgatgcaga
     1201 tgtccgcggc tgattcggat atggatgcca tcggtgtgaa aacatggccg ggcatgatca
     1261 gatcatggtg gatttcatcc gccaaaatca caacatcatt ggcggcacag atgtcgacga
     1321 cacgctccaa ttctgctttg gtccagacgc ggccgactgg attatgcggg ttgctgaaca
     1381 gcatcagctt tgtattcggt tctttggctt tctcagtcaa accctcaaaa tcaatttcat
     1441 aacgatcacc gttgcggatc aaagaattgt tgataagctt gcgtttcgta tttttgattg
     1501 acttcatgaa cggataatag accggcccca tgtaaaggac accctcgcct ggatcggtat
     1561 acgcccgaat agcggaatag aatgccgcga tgatgcctgg tgtacagata atccattcct
     1621 tcttgatttc ccaagcgtgg cggtttccca tccaattgat gattgcgcgg taatagtctt
     1681 cggtcggcag atcatagccc attacggctg tttcaatgta ttcctgcaaa ccctcgacga
     1741 tctccggcgc tgtctttatg tccaaatccg caacagaaaa aggaaaaaca tcctttccca
     1801 cattcggatt gcttctgtac atggctgccc atttttcgga ccctgtattg ctccgattga
     1861 cgggtgtttc gaaatcatac ttcatctatt catcctccca acattttact tacttttcat
     1921 tttatcactc gttttcacaa ttgccacccc taatcagatt ttaatccata gaaaatggag
     1981 aatccatttc ggagattctc catttttctc acatacaatc aaagactcac cttttagttt
     2041 tttctcttca gcattatgag gtaactttga taatttttgt tgccgcctga aaggttgtag
     2101 acttcctgga agcctaaatt gatcaagaca ttctgagcgg catttccggt cacacctttg
     2161 ttgcagtagg taactgtcgg gatatctttg gaaagctcac cggcgcgtgc gcgcagttcc
     2221 cctaacggga cgtggatcgc atcttggacg tggctcttct cgaagtcttt ggcggaacgc
     2281 gtgtcgatga cctggataga ttcgcctttt tgctgcattt cgaccnnnnn nnnnngccgg
     2341 attcgtggac gagaccgcgc tgtaggagcc gcgcgatgct cgtcgaggcg atggggtgag
     2401 ggtatcatga ccaaaaggca cggctgggcc ggcgtcatca aaggatttcc gtgtaaggcg
     2461 ttatccaatg ccatgcctgt ataatggata ggatcttttg tcgttgcgaa tggcggtgca
     2521 taggccaaat ccagatggaa caggtcttcc gctttcgcct tgaaagtgat ggcagtggca
     2581 aggacatcga tccgtttgtc cacaccttgc gatcctaaaa cttgtgcgcc taagactcgt
     2641 ccggtggctt tatccgccaa tgccttgatg atcagttcct tgccgccaag gtattcggcg
     2701 tggtccggct tgatgttgta taggacttcc acggaatagt cagcggcctt cgcttctttt
     2761 tcagtcatgc ccgtctggcc aacatgcaga tcgaatacgc ggaagatgcc ggtacccagc
     2821 accccgcgat gttccagatt tccgcctgtg atcacatctc cggcgatacg tcccatcttg
     2881 ttggccgtag agcctaatgg acggtaaatc ggtttgcctg taatggcgga gaagctttcg
     2941 gccacatcac cgaccgcata tacatcctgg atgtttgtct gcattttgct gttgacggca
     3001 accgcaccct tcgctccgat cgtgacgccg atttctttgg cgagctcagt gttcggtcgc
     3061 acgccagcag ccaggatgac aatatccgtt tcgatggtgg caccatatgt cgtcactact
     3121 tttttgacgg tccccttacc ttcgatggtc ttgaccgtat ccccgaggtg caaatgcacc
     3181 ccgttggcca tcagcacttc ttccacccgg aaagccatat ccgcgtccat cgacggcatc
     3241 acttgatttt ccagctgcac gatggtgaca tcccagcctt tttgtacgag ctgttcggtc
     3301 atttccagac cgatgaaccc ggcaccgatg agcagcgctt ttttgggttt gttcgcttcc
     3361 gaataacttt caatttcacg aaggtcctgg atgttgcgga cgtggaagac attatcgtat
     3421 tcagcctgat cgaacggcgg aggcgtcagc ggagccgcac ctgtcgcgaa aaccaacaca
     3481 tcataagcgt cctctttgac ttcgccggtc ttcagatctt taacttgaat ctttttggct
     3541 tcatgatcca cttttgtcac tctgtgttct gtaaagatgg agacatcata tctctttttg
     3601 aaccatttcg catctcgtgg tgtcaggcga tccactgaat cgacttcccc gccgattttg
     3661 tagggaatcc cgcagaca
//
LOCUS       DAOMUJ010000146         3654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7160_length_3654_cov_3.055571, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000146 DAOMUJ010000000
VERSION     DAOMUJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_3654_cov_3.055571"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..715)
                     /locus_tag="PLT73_04795"
     CDS             complement(59..715)
                     /locus_tag="PLT73_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM19730.1"
                     /translation="MLEAVLFDMDGVIIDTEPFFLRSESMLLKEFGVDTELEYHFRYQ
                     GTTHEYMWETMKNEFNLDAPVGELVERANVIRNRLMEEDGLQPIPGVIPFIARLHEAG
                     VPLAIASSSPLTDIHKAVKALDIEKYFSYFVSGESVAHSKPAPDIFLDAAENLNAKPE
                     NCVVIEDSKNGVASAHAAGCKCIGFRNLEFPPQDLSLASVIVTDFSDLDADYCRALVD
                     "
     gene            complement(816..2771)
                     /locus_tag="PLT73_04800"
     CDS             complement(816..2771)
                     /locus_tag="PLT73_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM19731.1"
                     /translation="MILLQGQNLARLFGASVLFDNIQITVQDHSRIALVGRNGAGKST
                     LLKILAGIEATDAGSVSKSKDVTIGYLDQHSAVDSSNTIWQEMLTIFAPLIQLSKEAE
                     QAAVALGDPDLLEDEEAYQKALERYDHLQHTLHEKNAYGYESEIRSVLHGFQFYEGDY
                     DRPISQLSGGQKTRLALAKILLEKNDLLILDEPTNHLDIDTLAWLENYLIGYNGSLLI
                     VSHDRYFLDKVATEVYEISRHKIQHYKGNYSYYLTEKAARLEQELKAYEKQQDEISKL
                     EDYVARNLARASTTKMAQSRRKRLEKMERMDRPKGDERSARFSFDIAKESGNVVLTLE
                     NGAVGYADKILADPINLDIRKQQAIAIVGPNGIGKSTLLKSIISEIPLLKGRVQLGAN
                     VDLGYYDQELGNLSKNKSVLAEIWDLHPTLNEKDVRSILGSFLFSGADVEKTIPALSG
                     GEKARLSLCKLALDQKNFLVLDEPTNHLDIDSKEVLENALIDYDGTLLFVSHDRYFIN
                     RIATNIVELSAEGSKLYLGDYDYYIEKKQQEAALAALQQAENATETNAAVEPLSKAKG
                     DYVSSKEKLKLERKLARQVEALEDELATVEEAIAAIELQLTEPEVYGDHEKVQQFNQQ
                     LLALQARQDELMQDWEDQTTALEELNE"
     gene            3121..>3654
                     /locus_tag="PLT73_04805"
     CDS             3121..>3654
                     /locus_tag="PLT73_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRM19732.1"
                     /translation="MSTNDIPKATARRLPIYHRYLRFLHNAGKKRVSSTELSEAVKVD
                     SATIRRDFSYFGALGKRGYGYDVEYLLDFFSKTLNQDRLTNVALIGVGNLGHALLNYN
                     FHVSNNVRISAAFDVNEGIVGKILSGVPVYPMTDMVEQLRIQQIDVAILTVPQEVAQD
                     ATNRLTEAGVRGIMNFTP"
BASE COUNT          887 a          880 c          882 g         1005 t
ORIGIN      
        1 ttaggtcaca gtgtttcctt aatcagggct gaacgaaccc gttcagcttt tcttattctt
       61 aatcgaccag cgctcggcag taatcggcat cgagatccga aaaatcggtg acgatgacgg
      121 aagccaacga cagatcctgc ggagggaact cgagattgcg gaaaccgatg catttgcagc
      181 cggccgcatg tgcagaggca actccgtttt tggagtcctc aatgacaacg cagttctccg
      241 gtttggcatt caagttttct gcagcatcca ggaagatatc gggcgcgggc ttggaatgcg
      301 caacgctttc ccccgaaaca aaatagctga aatatttttc gatgtccaat gcttttacag
      361 ccttatggat atccgtcaaa ggagaagagg aggcgattgc caaaggaaca ccggcttcat
      421 gcagacgtgc aatgaaggga atcacacccg gtatcggctg caggccgtct tcttccatca
      481 ggcgattgcg gatgacgttc gcccgttcca ccaattctcc taccggggca tccagattga
      541 attcattttt cattgtttcc cacatgtatt cgtgcgtggt cccttggtag cggaaatgat
      601 actccaactc ggtgtccact ccgaattcct tcaatagcat actttctgat cgcaaaaaga
      661 atggttccgt atcgatgatg acaccatcca tatcaaataa aacggcttcc aacatattcg
      721 tacctccata ttcctattat gcgtacttgt tcgagtgcaa cttaacaaaa gttccggcat
      781 tcctcaacta agacaggaaa cgcttatcgg cttcactatt cattcagttc ctcgagagcg
      841 gttgtctgat cttcccaatc ctgcatcagc tcgtcctgcc gtgcctgtag ggcgagcaac
      901 tgctggttga actgttggac tttttcatgg tcaccgtaga cttccggttc ggtcaattgc
      961 agctcgatcg cagcgattgc ctcctcgacg gtcgccaatt catcctccaa agcctccacc
     1021 tgccgagcca gttttctttc caatttcagt ttttccttgg aagaaacgta atcccctttt
     1081 gctttcgaaa gaggctcaac ggcagcgttt gtttccgtgg cgttttcggc ctgctgcaaa
     1141 gctgccaacg ccgcttcttg ctgtttcttt tcaatatagt aatcgtagtc cccgaggtat
     1201 aatttactgc cctcagcgga aagttcgaca atgtttgtcg caatccggtt gatgaaatag
     1261 cggtcgtggg atacgaaaag cagggtgccg tcataatcga tcaaggcatt ttcaagcact
     1321 tctttgctgt cgatgtccaa atggtttgtg ggttcatcga ggacaaggaa attcttttgg
     1381 tccagtgcca gcttgcagag cgaaagcctt gccttctcgc cgccgctcaa tgccggaatc
     1441 gttttttcga catcggcgcc ggagaacagg aagctcccca ggatactgcg cacatctttt
     1501 tcgttcagcg tgggatgaag atcccagatt tcggcgagaa cgctcttgtt tttggaaagg
     1561 ttgcctaatt cctggtcata ataaccgaga tcgacattgg cgccgagctg gactctgcct
     1621 ttcaaaagcg gaatctcgga aatgatcgac ttcagcaaag tggacttgcc gatcccgttc
     1681 ggcccgacga tcgcgatcgc ctgctgttta cggatgtcaa gattgatcgg atcagccagg
     1741 atcttatcgg catagccgac agccccgttt tccagcgtca agacgacatt tccgctttct
     1801 tttgcgatat cgaaagagaa gcgagcggaa cgctcatcgc ctttcggtct gtccatccgc
     1861 tccatttttt ccagacgttt gcggcggctt tgtgccattt tggtcgtgga agcgcgcgcc
     1921 agattgcggg cgacataatc ctccaatttg gatatttcat cctgttgctt ctcgtaagcc
     1981 ttcaattcct gctccaggcg ggcggctttc tcggtcagat aataggagta gttgcctttg
     2041 tagtgctgga tcttgtggcg gctgatttca tacacctccg tggcgacttt gtccaggaag
     2101 tatcggtcat gggaaacgat caaaagcgag ccgttgtaac cgatcagata attttccagc
     2161 caagccagcg tatcgatgtc cagatggttg gtgggctcat ccaaaatgag caggtcgttc
     2221 ttttcaagca ggattttggc caaggcaagc ctggttttct ggccgcctga caattgggaa
     2281 atcgggcgat cgtaatctcc ttcgtagaac tggaatccgt gaagcaccga acggatctcg
     2341 gattcatagc cgtacgcgtt cttttcgtgc aaagtatgct ggagatggtc atagcgttcc
     2401 agagcttttt ggtaggcttc ttcatcctcg agcaagtccg gatcccccaa agcgacggcg
     2461 gcttgttcag cttccttact taattggatg agcggggcaa aaatggtcaa catttcctgc
     2521 caaatcgtgt tggatgaatc gaccgcgcta tgctgatcca ggtagccgat cgttacgtcc
     2581 ttgctctttg aaacagagcc ggcatccgtt gcttcgatgc ctgccaaaat cttcagcaag
     2641 gtcgatttgc cggctccgtt tcggccgacg agtgcaatac gcgaatggtc ttgaacagta
     2701 atttgaatat tgtcgaataa gacgctcgcc ccaaaaaggc gcgccaaatt ttgtccttgc
     2761 agtaaaatca tatgtagtgt cctctttcta agcaaatgct ggcatttatc ccttccattt
     2821 tatcacagca tgcattgatt cgatagggac aagcaaagtt attgctatat tcacgtattt
     2881 gcacatgtga aaaaatgttg gcgactccgc ttacatttgt tacagtattt cacatgttgc
     2941 tcaaaaaagg cttcacaatc aagtccgatt tcgaacaaat tagtagattt ttcacaatca
     3001 ggtgatataa taggcagtat gaaatgaaag cttaagtatg tgaacatact ttttaaagtt
     3061 tgtcggaatc gcacaaacac tatgagttac gaaatgaaag aggaaagaag aggagaaaaa
     3121 atgtcgacga atgatattcc aaaagctacg gcaaggaggt tgccgattta ccaccgctat
     3181 ttgcgctttt tgcataacgc tggtaaaaaa cgtgtttcat ccactgagct cagtgaagca
     3241 gtaaaagtgg atagtgctac tatccgtagg gatttttcat attttggcgc actgggtaaa
     3301 agaggatacg gctatgatgt cgagtatttg ttggatttct tcagcaaaac cttgaatcag
     3361 gaccgtttga cgaacgttgc tttgatcggt gtgggtaatt tgggtcatgc cttgttgaac
     3421 tacaacttcc acgtaagcaa caatgtgcgc atcagtgcag catttgatgt caatgaaggc
     3481 attgttggta aaatcctcag tggtgtaccg gtgtacccca tgacggatat ggtggagcag
     3541 ttgcgtatcc aacagattga tgtagccatt ctgaccgttc cccaagaagt tgcgcaggat
     3601 gcaacgaacc gtttgaccga agcgggcgtt cgcggcatca tgaactttac cccg
//
LOCUS       DAOMUJ010000147         3646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7183_length_3646_cov_1.834865, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000147 DAOMUJ010000000
VERSION     DAOMUJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7183_length_3646_cov_1.834865"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..>1071
                     /locus_tag="PLT73_04810"
     CDS             462..>1071
                     /locus_tag="PLT73_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM19733.1"
                     /translation="MKKLFRVRASILTLMAATVLMACGNTEGNNADTSSDGSAASSTD
                     SINSDLPYIGIMQLTTHPALDQIGEGIIDQLEEAGYVDGETATIDFQNAQGDQSNMQS
                     IAERFVSNDADIMIGIATPAAQALANASSDIPIILGAITDPVAASLVQSMEKPGGNIT
                     GVSDKTPARDQFILIQQLLPDAKTVGILYSSAEDNSISSAKEA"
     assembly_gap    1072..1081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1082..1632
                     /locus_tag="PLT73_04815"
     CDS             <1082..1632
                     /locus_tag="PLT73_04815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM19734.1"
                     /translation="YGMALYALHHEEWMSCILRCDDQEEALGGLFFNSAKEAEEIAAS
                     LGLKTVTKTVTSTNDIAQVAESLASEVDAIYVPTDNTIASGLATLIPVADAYNIPVFP
                     AVDTMVEEGGLATIGLNQYALGTQTGAVAADILAGADPATYPVQYATGIQLVINRAKA
                     DQLGITIPDDIAQNAIFVGEGE"
     gene            1638..2534
                     /locus_tag="PLT73_04820"
     CDS             1638..2534
                     /locus_tag="PLT73_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003649489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM19735.1"
                     /translation="MNMIISAVSQGLLWSILALGLFVSFRILDLADMTTEGAFPLGAA
                     VAVQAITLGANPYIAIIFAIIAGSSAGLITGFLITKLQIPSLLAGILTMTGLYSINLR
                     VMGRANISLLGKNTIFTVFENMNLPRHFDTITLGLLVVVMIIGLYVIFFKTEFGQAVI
                     ATGDNEAMARSLGISTNTMKIVGLMISNGIVALAGSIIAQDNGYADISMGIGTIVIGL
                     ASIIIGEVLFTNVSFKMRLVCLMLGSVLYRLIMVMVLEAGMNPNDFKLISAVLLTFFL
                     ALPKIKETYYMYSLRKKVKRND"
     gene            2527..3321
                     /locus_tag="PLT73_04825"
     CDS             2527..3321
                     /locus_tag="PLT73_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19736.1"
                     /translation="MTNQEAMLTMSHVSKTFYPGSPNQVNALQDVNLTVRQGDFITLV
                     GGNGAGKSTLLNAISGNFLPDTGDIIVNGQNVTFFKEDKRAPYISRVFQDPKMGTAPR
                     MTVQENLSLAYRRGQKRTLRFGTSHEEKAFFKEELKKLGLGLENRMGSDIGLLSGGQR
                     QAIALLMATMKRPDILLLDEHTAALDPKTAKLVLELTNQQVIDNNLTSIMITHNLQDA
                     IQYGNRMLVLHHGQIMNDYDAETKKTLTPEKVFAELQELSIQDILV"
     gene            3341..>3646
                     /locus_tag="PLT73_04830"
     CDS             3341..>3646
                     /locus_tag="PLT73_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRM19737.1"
                     /translation="MWHPGDAIGFISCSNGLTNNDRSLEIVSSLSRKLKDDFGLETVM
                     SDVLFLDKETQQTEAPASRAGALLSLYSNASVKAIFDLSGGDLANEILPYLDFGIIKR
                     "
BASE COUNT          975 a          825 c          891 g          945 t
ORIGIN      
        1 atcaaaggat agatgaacaa gcacggtaca ggatcctcct gctcccttgc ttgtttattt
       61 tttgatccga agcttatgga aggttttatg agaatacctc atctttttct aatccaactc
      121 ttgactttgt tttttgcatt gggtactatt ggattatcac taaataaaag gagcaatcaa
      181 tcatgtcagc atatacacgc gtttccaaaa tgaatttttt tgattggcaa agccacgtgg
      241 agtaggttgt tctttgtgac gtattgattg tccgtagata cagcctcaga tgaaacatct
      301 gaacagtatc tatgtggatt actaaagata tttaagccgc cacatagaca atatcggtct
      361 gtgtggcgtt ttctgtattc agccgccata cttcgttcag atggcggctt ttttatcacc
      421 tttttacaga ataagaagca gaaaaattgg aggaatcaga aatgaaaaaa ttattcagag
      481 tacgcgcaag catcttgacc ttaatggcag cgacagtatt aatggcatgc ggcaatacgg
      541 agggcaataa tgcggacacc tcttcggacg gttctgcagc aagctccacc gacagcatca
      601 actcggattt gccttacatc ggtattatgc aattgactac ccatccggca ctcgatcaga
      661 ttggggaagg tattatcgac caattggaag aagccggcta tgtggatggc gaaacagcaa
      721 cgatcgattt ccagaatgcg caaggcgatc agtccaatat gcagtcgata gcggagcggt
      781 tcgtttcgaa tgatgcggat atcatgatcg gcatcgctac ccctgcagca caagcgttgg
      841 cgaacgcatc cagcgatatt ccgatcattt tgggagccat tacggatccg gtggctgcca
      901 gccttgtgca gtcaatggaa aaaccgggcg gcaacatcac cggcgtcagc gacaagactc
      961 cggcccgcga tcagtttatt ttgattcaac aattgcttcc tgatgccaaa acggtcggta
     1021 tcctttactc atcagcagag gacaattcaa tcagttccgc taaagaggct gnnnnnnnnn
     1081 nattacggta tggcattgta tgccttacat cacgaagaat ggatgagctg cattcttcgc
     1141 tgtgatgacc aagaagaagc acttggaggg ttgtttttta attctgctaa agaggctgaa
     1201 gaaattgctg cttcattagg cttgaagacc gtcacgaaaa cagtcacttc aacgaacgat
     1261 atcgcgcaag tcgctgaaag ccttgcttcc gaagtggatg ccatctacgt accgacagat
     1321 aatactatcg caagcggact ggctacattg atccctgttg cggacgcgta taatatccct
     1381 gtattcccgg ctgtggacac aatggttgaa gaaggcggat tagcgacaat cggtttgaat
     1441 caatatgcat tgggaactca aacgggtgct gttgcggctg atatcctggc tggggccgat
     1501 cccgctactt acccggtaca gtacgcgacc gggatccaac tggtcatcaa tcgagcaaaa
     1561 gcggaccaat tgggcatcac gattcctgat gatatcgcac agaatgccat ctttgtagga
     1621 gaaggagagt agatttaatg aacatgatta tttccgccgt ctcccaaggg ttgctctgga
     1681 gtattctggc acttggttta tttgtaagtt tccgtatttt agatttagcc gatatgacaa
     1741 ccgagggggc gttccctctc ggtgcggctg tggctgttca agccattact ttaggagcga
     1801 atccgtatat cgcgattata tttgccatca ttgccggcag ttcagccgga ctgatcactg
     1861 gctttttgat cacaaaactg cagattccca gccttttggc aggtattctg acgatgaccg
     1921 gcctctattc catcaacctg cgcgtgatgg gaagagcaaa catcagtctg ttgggcaaga
     1981 atacaatctt cacggttttc gaaaatatga atcttcccag acatttcgac accattacct
     2041 taggcttgtt ggtagtcgtc atgatcattg ggctctatgt catcttcttc aaaacggaat
     2101 tcggtcaagc tgtcatcgca acgggtgaca atgaagcgat ggcccgctcg ctgggtattt
     2161 ccacaaacac gatgaaaatc gtcggattga tgatttcgaa cgggatcgtt gctttggcgg
     2221 ggtccatcat cgcccaggac aatggctacg ccgacatcag tatgggtatc ggaacgattg
     2281 tcattggtct tgcttcgatc atcatcggcg aagtattgtt cacgaatgtc tctttcaaaa
     2341 tgcgtttggt ttgcctgatg ctgggttcgg tcctgtaccg actgatcatg gtgatggtat
     2401 tggaggcggg aatgaacccg aatgacttca agttgatttc agctgtattg ttgaccttct
     2461 tcttggcgtt gccgaaaatc aaggaaacct actacatgta ttcattgcga aagaaggtga
     2521 agcgaaatga ctaatcagga agcgatgttg acgatgtcac atgtctccaa aacgttctac
     2581 cctggttcgc ccaatcaggt caacgcactg caggacgtca atctaacggt cagacaaggc
     2641 gatttcatca ctttggtcgg aggcaacgga gccggcaaat caaccttgtt gaatgccatt
     2701 tccggaaact tcctgcctga tacaggcgat atcatcgtaa acggacaaaa tgttaccttc
     2761 ttcaaagaag acaaacgagc gccttacatc agccgggtct tccaggatcc aaaaatgggg
     2821 acggccccac ggatgacggt gcaggagaat ttatccttgg cataccgccg aggccaaaaa
     2881 cggacgctgc gtttcggaac ttcgcatgaa gaaaaagcat ttttcaaaga ggaactgaaa
     2941 aaattgggct taggcctgga aaatcggatg ggctcggata tcgggctgtt gtcgggcggt
     3001 cagaggcaag ccatcgctct gctgatggca acgatgaaac gaccggatat cctgctgctg
     3061 gatgaacata cggcagcctt ggatccgaaa acggccaaat tggttctgga attgacgaat
     3121 caacaggtaa tcgacaacaa cctcaccagc atcatgatca cccacaatct gcaggacgcg
     3181 atccaatacg gaaaccgcat gctggtactg catcacggtc aaatcatgaa cgattacgac
     3241 gctgaaacga agaaaacatt gacgcccgaa aaagtatttg ctgaacttca ggaattatcc
     3301 atccaagata tcttggtctg acagaaaggt agtgatcttt atgtggcatc cgggagatgc
     3361 gatcggcttc atcagctgtt cgaacggatt aacgaataat gatcgctcgt tggaaatcgt
     3421 ttcgtcgctc agccggaaac tcaaggatga ttttggcctt gagaccgtaa tgagtgatgt
     3481 ccttttcctg gataaagaga cacagcaaac tgaggcccct gcatcccgtg caggggcttt
     3541 gttgtccctt tactccaacg cgagcgtaaa ggcgatattt gacttatccg gcggggattt
     3601 ggccaatgaa atattgcctt atctcgattt cgggatcatc aaaaga
//
LOCUS       DAOMUJ010000148         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7195_length_3643_cov_2.583612, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000148 DAOMUJ010000000
VERSION     DAOMUJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_3643_cov_2.583612"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLT73_04835"
     CDS             complement(<1..656)
                     /locus_tag="PLT73_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM19738.1"
                     /translation="MKKSKLFGGLVLTASLFLAACGNGGTTADSSAAESSSTVSAEPT
                     TVKIGLVSESAVEIWEAVAERLEDENIDLEIVKFTDYNQPNIALDNGELDLNAFQHVA
                     FLENYNANNDADLTPIGFTFVSPLGIYSDKYADYSEIQDGDTIAIPNDVTNGGRALLL
                     LQAIDLITLDNAAGTAPTVNDIIENPKNLNIEELDAAQLPRSLEDAGAAVINTNFAVD
                     A"
     gene            complement(735..1589)
                     /locus_tag="PLT73_04840"
     CDS             complement(735..1589)
                     /locus_tag="PLT73_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM19739.1"
                     /translation="MKKKNVLFSGVAALTLFLAACGSSDSNTDSAADTAASEAEAVKI
                     GVVSEVEVEVWEDVASRLEAKGIELEIVQFSDYVQPNVALENGGIDLNAFQHVAYLED
                     FNANNESDLTPIGFTYVSPLGLYSETVTDYADIADGAKIAIPNDVTNGGRALLLLQAT
                     GLIKVDEAKGTTPTVNDITENPKNISFEELDAAQVARSLPDVDAAIINTNYATDSGLN
                     PKEDALFLDTDNIASVADVYKNIVAARSEDVDNETYKQVVAEYQTSETAALLDDVTEG
                     NDVPAWEQ"
     gene            complement(1622..2302)
                     /locus_tag="PLT73_04845"
     CDS             complement(1622..2302)
                     /locus_tag="PLT73_04845"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRM19740.1"
                     /translation="MNTFITTFLPNVSEIWDEVLQSTWETLYMTLVAGLIAGILGVIL
                     GVILVVTEDGGILESRHLYNVLDKLVNIFRSLPFIILMALIVPFTRLVVGTSIGTTAS
                     IVPLVVATVPFYARQIQNALVEVDAGVIEAAQSMGASPGEIIFRVYLKEGLPGIIRVS
                     SVTIINLIGLTAMAGAIGGGGLGNLAITRGYNRFQNDVTLVATIIILIIVFISQAIGN
                     ALVKKVSH"
     gene            complement(2299..3375)
                     /locus_tag="PLT73_04850"
     CDS             complement(2299..3375)
                     /locus_tag="PLT73_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006418964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19741.1"
                     /translation="MISLQNISVTFKTDKNKAIHAVQHVSLEVDKGDVYGIVGYSGAG
                     KSTLVRTINLLQRPTEGKVIVSGKDMMALNDKELRESRKKIGMIFQHFNLMRSRTIHD
                     NVAFPLKNSSLSKQEINDKVAELLSLVGLSEKAKAYPSQLSGGQKQRVAIARALANDP
                     EVLLCDEATSALDPKTTSSILTLLKELNKRLNLTIVIITHEMQVVKEICNKVAVMENG
                     GVIEYGSILDIFTKPQNQLTKDFIDTATHVEHGIETVITHPTILNLNEHDVLTKLSFV
                     GGSTGEPLIAKLANTFNVQGNILFGNVEILQDTPVGTLLLVLSGTPEAIAEAVRYLQD
                     NGVTVEIITEEIIAEKKNKGGDEE"
BASE COUNT          782 a          883 c          915 g         1063 t
ORIGIN      
        1 gcatcgacgg cgaagttggt gttgatgacc gcagcgcctg catcttcaag tgaacgcggc
       61 aattgagcgg catccaattc ttcgatgttc aggttcttag gattttcgat gatgtcgtta
      121 acggttggtg ctgtgcctgc tgcattgtcc aaagtgatca agtcaatggc ttgcaacagc
      181 aacaaagcgc gtccgccgtt ggttacgtca ttcgggatag cgatcgtgtc accgtcttga
      241 atttcactgt aatctgcata tttgtcagag tagatcccca gtggagacac aaaagtgaat
      301 ccgattggtg tcaagtctgc gtcgttgttt gcgttgtagt tttccaggaa agctacgtgt
      361 tggaaggcgt tcaagtcgag ttcgccgttg tccaaagcga tgttcggttg gttgtaatca
      421 gtgaatttga cgatttcaag gtcgatgttc tcatcttcca gacgctctgc gacagcttcc
      481 cagatttcga ctgcggattc gctgaccaga ccgattttga ctgtagttgg ttctgctgaa
      541 actgtgctgc ttgactctgc agcggacgag tcagcggttg tgccgccgtt tccgcatgct
      601 gccaggaata gtgaagctgt aaggactagg ccaccaaata atttactctt tttcatatga
      661 atttcctccg atttgttttt tatattttta tatcacgtct gcaaacgtga tttttgtaaa
      721 caagtgaatg ggtcttactg ttcccaagcg ggtacgtcat tgccttcagt cacatcatcc
      781 agaagtgctg ctgtttcgga tgtttggtat tccgcaacga cttgcttgta ggtttcgttg
      841 tcgacatctt cagaacgtgc agctacgatg tttttgtaga cgtctgcaac gcttgcgatg
      901 ttgtcagtat ccaagaagag cgcgtcttct ttaggattca ggccggaatc ggtagcatag
      961 tttgtgttga tgatcgctgc atccacatcc ggaagggagc gtgcaacttg tgcggcatcc
     1021 aattcttcaa agctgatgtt cttcggattt tcggtgatgt cattgactgt cggtgtcgtt
     1081 cctttcgctt cgtcgacttt gatcaggccg gttgcttgca acagcaagag tgcgcgtccg
     1141 ccgttggtca cgtcgttagg gatggcgatt ttcgctccat cggcgatgtc cgcatagtcc
     1201 gtcactgttt cagaatacaa accaagtgga gaaacataag tgaagccgat tggagtcaag
     1261 tccgactcgt tgttggcatt gaaatcttca agataagcta cgtgctggaa cgcattcagg
     1321 tcgataccgc cattttccaa ggccacgttg ggctggacgt agtcgctgaa ctggacgatt
     1381 tccaattcga ttcctttggc ttccaaacgg ctggctacat cttcccagac ttcaacttcg
     1441 acttcgctga ctacgccgat tttcactgct tcagcttctg aagcggccgt gtctgctgct
     1501 gagtccgtgt tcgagtcgct gcttccgcaa gctgctaaaa atagggtaag tgctgcaaca
     1561 cctgagaata atacgttttt ctttttcatg attgatttcc tccgcttttg ttttttttat
     1621 attagtgact aacttttttg accaaagcat ttccgatggc ttggctgatg aatacgatga
     1681 tcaggatgat gatggttgcg accaaggtca cgtcgttctg gaatcggttg tagccacgcg
     1741 tgatggctag gttacccaag ccgcctccgc cgatggctcc agccattgcc gtcaaaccga
     1801 tcaggttgat gatcgttacg gatgatacgc ggatgatgcc tggcaagcct tccttcaggt
     1861 agacgcggaa gatgatttcc ccagggctgg cacccattga ctgtgccgct tcgatgacgc
     1921 ctgcatcgac ttccaccaat gcattttgga tctgacgggc atagaaggga actgtcgcta
     1981 caaccagcgg tacaatcgat gcggtcgttc cgatcgaggt accgaccacg agacgtgtga
     2041 acggtacgat cagtgccatc agaatgatga agggcagtga tcggaagata ttgacgagct
     2101 tgtccagcac gttgtacagg tgtctgctct ccaggatgcc cccgtcttcg gtgacaacca
     2161 ggatgacgcc aaggataact cccagtatac ctgcaatcag tccggccacg agcgtcatgt
     2221 ataaggtctc ccatgtgctc tggagcactt catcccagat ttcagagacg ttaggcagaa
     2281 atgttgtgat gaatgtgttc attcttcgtc tcctccttta tttttctttt cggcgatgat
     2341 ttcttcggtg atgatttcca ccgttacacc attatcctgc aagtagcgga cggcttccgc
     2401 gatcgcttcc ggtgtcccgc tcagaacgag aagcagggtg ccgactggtg tatcctggag
     2461 tatttctacg tttccgaaga ggatattgcc ttggacattg aatgtattag ccagttttgc
     2521 gataagtggt tcgcctgtgg aaccgccgac gaatgagagc ttcgtcagta catcatgttc
     2581 gttcaggttc aggatagtcg ggtgggtgat gaccgtttcg atgccgtgtt cgacatgcgt
     2641 cgctgtatcg atgaagtcct tcgtcaattg attctgcggc ttcgtgaaga tatccaggat
     2701 cgatccgtat tcgatcacgc cgccgttttc catcacagcc actttgttgc agatttcctt
     2761 cacgacctgc atctcatggg tgatgatgac gatcgtcaag ttcagtcttt tgttcagttc
     2821 cttcaataaa gtcagtatcg aagaagtcgt tttcggatcc agcgcgctgg tcgcttcgtc
     2881 acaaagcagc acttccgggt cgttcgccaa agcgcgggcg atggccacac gctgcttttg
     2941 cccgccggac aattgggaag ggtatgcttt ggctttttcg gacaaaccta ctaatgaaag
     3001 aagttccgct accttgtcgt tgatttcctg tttgcttaag gaagagttct tcaacggaaa
     3061 tgcgacgttg tcgtgaatgg tgcgggagcg catcaggttg aaatgctgga aaatcatccc
     3121 aatttttttg cgcgattcac gcagttcctt gtcgttcaat gccatcatgt cttttccgct
     3181 cacgatgact tttccttcag tcgggcgctg cagaagattg atggtcctta ctaaggtact
     3241 tttgccggcg ccactgtatc cgacgatgcc gtagacgtcg cccttatcga cttccagcga
     3301 aacatgctgc acggcatgga tggctttgtt tttgtcagtt ttaaaagtca ctgagatatt
     3361 ctgtagactg atcatgtatc tttcctcctc taataaaaaa aacatccatc ctacatcaaa
     3421 aaaatatgac gttaaggacg aatgcggatg cttcgtgtta ccacctttgt tcgaatcccc
     3481 ctcacggcag gattcctcca tgagtgaagg cttgcttcac tcgtgcacga taaagggtgc
     3541 tcccttgaag gacttaccgc ttgtgcagct catccttccc gctccgaggc catcttcaac
     3601 accgttccgt atgcctgttc tcatcaaacc aggctcgctg tga
//
LOCUS       DAOMUJ010000149         3639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7214_length_3639_cov_1.832031, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000149 DAOMUJ010000000
VERSION     DAOMUJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7214_length_3639_cov_1.832031"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLT73_04855"
     CDS             <1..827
                     /locus_tag="PLT73_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19742.1"
                     /translation="QLFEKERRKIPFGPSWRLGDTFSKRKLSGSLGFTGHFPEKIFAT
                     GSVCLLLIALTGLVQAKSGENADVQELREQIAASQSKMEEINRDASARSRAINNIEGL
                     EREVVLFAHDADITFIGDSVLLSVSDQLVTIFGRAVVEGAVSRQLYQTTEVIADLKKR
                     EQLHDTVVVFLGSNGTFTRTQMESFIEEIGTSKDLFFLTTNVPRIWKDSVNEQLELAE
                     STHSNVHLLDWNAYSSGHGDWLLEDQVHPNPTGAQQLALFVAEQIYQELNDENGSN"
     gene            830..1759
                     /gene="rnz"
                     /locus_tag="PLT73_04860"
     CDS             830..1759
                     /gene="rnz"
                     /locus_tag="PLT73_04860"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRM19743.1"
                     /translation="MKIQFLGTGAGVPSTSRNLSSIALKLLDERNAIWLFDCGEGTQQ
                     RVLKTTIRPRKVEKIFITHLHGDHIFGLPGFLSSRAFQGGNTPLNIYGPVGIKEFVLT
                     SLRISQSHLRYPIFFHEITEDGVVFEDEQFRVSCAKLNHGIISYGYRVEEADYPGELQ
                     ADKLKEMDVPAGPLYGKLKNGETVVLPDGRTINGLDFIGKTTKGRVVTILGDTRRTKN
                     TVVLARDADVLVHESTFGKEDQAIASDYFHSTCIDAANVAKEANVKQLYLTHISARYL
                     NGNAHQLQRDARKVFQNTKLVNDYDEFDIALMK"
     gene            1883..2293
                     /locus_tag="PLT73_04865"
     CDS             1883..2293
                     /locus_tag="PLT73_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HRM19744.1"
                     /translation="MKNQWRLVAGIILIIIIVLFAVFNVDSVPVNFGFVVLDGPLIIV
                     ILVSLLMGSLITLLVATGSATKKNKEFKQMRAEVDAKSKEIQTAVDTAKAGYEQQLSD
                     LRMQLSQKDSKINSLEEELIKKLTVGNPDQPSKI"
     gene            2601..>3639
                     /gene="recJ"
                     /locus_tag="PLT73_04870"
     CDS             2601..>3639
                     /gene="recJ"
                     /locus_tag="PLT73_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRM19745.1"
                     /translation="MEFHDPYLLHDMDKAVHRLTEAIESGEEIVVYGDYDADGITSTC
                     ILVETIEVLGGNVGYYLPNRFTDGYGPNAAAFKKLIENGAQLILTCDNGVSGHEPIAM
                     AKKMGVDVIVSDHHELPAVLPDAYAVIHPKHPAGSYPFPDLSGAGVALKIAAALLGEM
                     PFESLDLAAIGTVADLVSLTGENRWIVKQGLQVMKQAHRLGLAALMEAAGIDTAVLDE
                     ESIGFIIGPRLNAVGRLEDAGPGAELMLSFDEEKIAELVAYIQEKNVERQSIVQSIHE
                     EARIQLAKNEILPDVVVLGNKNWHQGVLGIVASKLVEEFGRPTVLFNIDEKTGVAKGS
                     ARSTEALDIYAA"
BASE COUNT         1043 a          755 c          920 g          921 t
ORIGIN      
        1 accaactatt cgaaaaagag cggcggaaaa taccgtttgg gccgtcttgg cgtcttgggg
       61 atactttttc caaaagaaaa ctttcaggat ccctcggttt caccgggcat tttccggaaa
      121 aaatctttgc aaccggcagt gtttgcctgc tgctgattgc actgactggt ttggtccagg
      181 caaaatccgg cgaaaatgcc gatgtgcagg aattgcgaga gcagatcgca gccagccaga
      241 gtaagatgga agaaatcaat cgtgatgctt ccgcccgcag cagggccatc aacaatattg
      301 aagggctgga acgagaggta gttttatttg cccatgatgc tgatattacc ttcataggcg
      361 actctgtgtt gctctcggtt tcggatcagc tggttactat ttttggtcgg gcagttgtcg
      421 aaggtgcagt cagcagacag ctttaccaaa ctactgaggt gattgctgat ttaaaaaaac
      481 gggagcaatt acatgatacg gttgttgtct ttcttggttc caacgggact ttcacccgta
      541 cgcaaatgga aagcttcata gaggaaatcg ggacatccaa ggatttgttt tttctcacca
      601 cgaatgttcc gagaatctgg aaggacagcg tgaatgagca attggaactt gccgaaagca
      661 cgcattccaa tgtccatctt ctggattgga atgcctacag cagcggccat ggcgattggc
      721 ttctggagga ccaagtccat ccgaatccga ctggcgcgca acaattagcc ctttttgtgg
      781 cagagcaaat ctatcaagaa ttaaatgatg aaaatgggag caattgatta tgaaaattca
      841 atttttaggc acaggagcag gtgttccttc caccagcaga aatctttcca gtatagcatt
      901 gaagctgttg gatgaacgca atgccatctg gctttttgac tgcggggagg gtacacagca
      961 gcgtgtactc aaaacaacca tccgacccag gaaagtggaa aaaatcttca tcacccatct
     1021 gcatggagat catatattcg gactgcctgg ctttttgagc agtcgggctt ttcaaggcgg
     1081 caatactccc ttgaacatct atggccctgt cggaataaag gaatttgtgt tgaccagtct
     1141 gcgtatatcc cagtcgcact tgcgctatcc gatctttttc cacgaaataa cggaagatgg
     1201 cgttgtgttt gaagatgagc aatttcgcgt cagttgtgca aaactgaatc atggtatcat
     1261 ttcgtatgga taccgtgttg aagaagcaga ctatccagga gaattgcagg ccgataaact
     1321 caaagagatg gatgttcccg caggaccgct ctatggaaaa ctcaagaacg gcgagactgt
     1381 tgttttgcct gacggaagaa ccatcaacgg actggacttt attggaaaaa caactaaagg
     1441 acgcgtcgta acgattttag gcgacacaag aagaacgaaa aatacagtcg ttttagccag
     1501 agatgccgat gtgttggttc atgagagtac ctttgggaaa gaagaccaag cgatagcttc
     1561 tgattatttc cactcaacgt gcatcgatgc cgctaatgta gcaaaagaag caaacgtaaa
     1621 gcaactttat ttgacccaca tcagtgcgcg ttacttgaac ggcaatgcgc atcagctgca
     1681 gcgggatgcc cgtaaagtct ttcagaatac aaaacttgtc aatgactatg acgagttcga
     1741 cattgctctg atgaaatgat tgccggaacc ctgataacca ggacatactt actattgttc
     1801 tcaagggttg taagttgacg atgcatgggc aaaaagatga taataagagt gtgtaaacat
     1861 caaaatgagt ggggcgatta taatgaaaaa tcaatggaga ttagtagcag gtattatttt
     1921 gatcatcatc attgtgttgt ttgccgtttt taacgttgat tctgtgccgg tcaattttgg
     1981 gttcgtcgtc ttggatggac ctctgatcat cgtcattctt gtttcacttc tgatgggctc
     2041 gctcatcact ttattggttg cgacagggag tgcgacgaag aagaacaaag agttcaaaca
     2101 gatgcgtgcc gaagtcgacg caaaaagcaa agagatccaa acagccgtag atactgcaaa
     2161 agccggatac gaacagcaac tgagcgatct gcggatgcag ttatcccaga aagacagcaa
     2221 aatcaattcg cttgaagaag agttgataaa aaagctgact gttggtaacc cggatcaacc
     2281 gagcaaaatt tgataaaaac agagctggag attcatttga tggctacttc aagttagaaa
     2341 gaaaagacta gaacgggttt agcttctagt cttttttcgg ttaaggaagc tgcagtgctt
     2401 ctatatagtt tacaaagtaa ggagtcggta aattgttatc gtcaaagatg aattggaaaa
     2461 tacgcaaatc agaaattgat gcatctataa gcctgggaat cagtcaggct acgggcttat
     2521 cagaaaagtt cgtcctactg tgcatgcagc gcggacttga aacaaaggaa caaatcacag
     2581 cattcgtcga agggacccaa atggaattcc atgatccata tttgctgcac gatatggata
     2641 aagccgtcca tcgtttgact gaagctattg aatcagggga agaaatcgtt gtttatggtg
     2701 attacgatgc ggatggcatc acaagtactt gcatactcgt ggaaaccatt gaagtgttgg
     2761 gaggcaatgt cggttattat ttgccgaatc gcttcacgga tggttacggc ccaaatgctg
     2821 ccgcattcaa aaaactgatc gaaaacgggg cgcaactgat cctgacttgc gataacggag
     2881 tttccggtca cgaaccaata gcgatggcaa aaaaaatggg ggtagacgtc attgtttccg
     2941 atcaccatga attgccggct gtcttgcctg atgcatatgc tgtcatccac ccgaaacatc
     3001 ctgccgggtc ctatcctttc ccggatcttt ccggagctgg cgtcgcccta aaaatcgcgg
     3061 cggcccttct gggggaaatg ccctttgaat cacttgacct ggctgctatc ggcactgttg
     3121 cggaccttgt tagtttgaca ggcgaaaatc gctggatcgt gaaacaagga ctacaagtca
     3181 tgaaacaagc gcatcggctc gggctggctg cattgatgga agccgctgga atagatacgg
     3241 ccgttttgga cgaagagagc atcggtttca tcatcggacc acgtttgaat gcagtcggtc
     3301 gattggaaga tgcgggtccg ggcgctgaat tgatgctgtc attcgacgaa gaaaaaattg
     3361 ctgaattggt tgcctatatt caggaaaaaa acgtcgagag acaaagcata gttcagtcca
     3421 tccatgaaga agcccgtatc caattggcga agaatgaaat tttgccggac gttgttgtgt
     3481 taggcaacaa gaattggcat cagggcgttc tcggaatcgt ggcaagcaaa ttggtcgagg
     3541 aatttggacg gccaaccgta ttatttaata tcgacgaaaa aaccggtgtc gcgaaagggt
     3601 cagcccgcag caccgaggcg ttggacattt atgctgcat
//
LOCUS       DAOMUJ010000150         3634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7229_length_3634_cov_2.332495, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000150 DAOMUJ010000000
VERSION     DAOMUJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7229_length_3634_cov_2.332495"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLT73_04875"
     CDS             complement(<1..570)
                     /locus_tag="PLT73_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM19746.1"
                     /translation="MIYFDNSATTIMYPEVLDTYRKVNEQFFGNPSSLHRIGNEADAL
                     LQQSRKQIAQLLAVQPDEIFFTSGGTESDNWAIKGTAMEKFHAGKHMIASSVEHPAVS
                     KSLEQLGKLGFEITYLPVDTDGIISVEELGKAIRKDTILVSVMAINNEVGSIQPIEAI
                     GELLENYPWVHFHVDAVQAVSECVPLIQHP"
     gene            complement(745..2463)
                     /locus_tag="PLT73_04880"
     CDS             complement(745..2463)
                     /locus_tag="PLT73_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HRM19747.1"
                     /translation="MEFIYWLVAIILLVLVGYGAIMYLTRQQANRIKAIDEKKQKAMA
                     IPVADNLFTLKNMNLTGQTKRTYESWQATWQTITRFQYPEIEAALVSAEQYIQRMNFI
                     KAKQAISQADQLIDETKNSVEKVNKALEKLLESAQENRKELEEIQERYNKIRKQLLAH
                     SFTFGPAIETLEKNLNYMELDFTKFNSLTNEGDHMEAKEILSRIEQDLLVMEEVVEKI
                     PELNEKIKTEYEEQVSDLKDGYQRLLDEKYIFEGVDVSAEIAAVEKEIQEAKDSIGLA
                     DINEAGKKMDKVERDIEAVYAIMEKEMEAKEFVGRHTANLQKKMDHVLQSNRYVLLEI
                     DRVSQNYFLNKNELGRAQEFEEQLLKENEALRYYDKMMKEHEISYSVVREYYEKISQR
                     LSEIDKEQSELVSNLSDLRNREKEIKDSIDLYELDMRNMKRTIEKYHLPGLPKIYLDL
                     FFSVTDRIEDLASKLNRVKIDMDEIDAISKMCEEDIEMLDNQTQAIVDNAMLTEYMIQ
                     YANRFRHSHVEIENAINKALVLFHREYDYEGALEAIRIPLNRIEAGAARKVEESYQEE
                     KNRRYY"
     gene            2878..3489
                     /gene="rpsD"
                     /locus_tag="PLT73_04885"
     CDS             2878..3489
                     /gene="rpsD"
                     /locus_tag="PLT73_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRM19748.1"
                     /translation="MSRYTGPSWKQARRLGISLLGTGKELERRPYVPGQHGPNNRKKL
                     SEYAMQLQEKQKLRHMYGMNERQFATLFKKAGKIKEGKQGVNFMILLEQRLDNVVYRL
                     GLASTRRQARQLVNHGHVTVDGKRVDIPSYEVAVGQVIGLREKSKNLVVVKAAAEALF
                     GRPDFITFDEEKLEGSLNRLPVREELPAEIDESFVVEYYNKLG"
BASE COUNT          943 a          845 c          709 g         1137 t
ORIGIN      
        1 aggatgttgg atcagcggca cgcattcact cacagcctgc acggcatcta cgtggaaatg
       61 aacccaagga taattttcca acagttctcc gatagcctcg attggctgga tgctgccgac
      121 ctcattattg attgccatga cactcaccag gatcgtatct tttcttatcg ctttcccaag
      181 ctcctcaacg gatatgattc cgtcagtatc gacaggcaga taagtaattt caaatcccaa
      241 tttccccaac tgttccagcg attttgagac ggcaggatgt tcgacagaag aggcgatcat
      301 gtgttttccg gcgtggaact tttccattgc agtacctttg attgcccagt tatcgctctc
      361 tgtaccgccg ctcgtgaaga aaatctcatc cggctgtaca gccagcaatt gcgcaatttg
      421 tttgcgcgat tgttggagca acgcatctgc ctcattacct attctgtgca aactggaagg
      481 attgccaaaa aattgttcat tgacctttcg gtacgtatcc aaaacctctg gatacattat
      541 cgttgttgca ctgttgtcaa aataaatcat ctatatttac acttccttaa taactttgca
      601 cccttagcgg gacatcctat ccattatata gaaaatgtct gaccattaaa agcttacggt
      661 ttaattttat tcccctctca taaaagagtg tccaaaaagg ggccgagaca atgtcccgac
      721 ccctgcgtct tattgattct gtatttaata gtaacggcga tttttttctt cctgatagga
      781 ttcttcgacc ttgcgggcag cccctgcttc tatacggttc aaaggaattc gtatggcttc
      841 caacgcacct tcatagtcat attcgcggtg gaaaagcact aaagctttgt tgatggcatt
      901 ttcaatttcc acatgtgaat gacggaaacg gtttgcgtac tggatcatat attcagtcag
      961 catcgcattg tccacaattg cttgggtttg gttatccaac atttcaatat cctcttcgca
     1021 catcttcgaa atcgcatcga tttcgtccat gtcaatcttc acgcggttca acttggaagc
     1081 caaatcttcg atgcgatccg tgactgagaa gaaaagatcc aaatagattt tcggtaaacc
     1141 gggcagatgg tatttttcga ttgtgcgctt catattgcgc atatccaatt cgtagagatc
     1201 gatgctgtcc ttgatttcct tttcgcgatt gcgcaaatca ctgaggttgc tgaccaactc
     1261 agattgttct ttgtcgattt cggaaagacg ttgactgatt ttttcataat actcgcggac
     1321 aaccgagtaa gaaatttcgt gttccttcat catcttgtcg taataacgca aggcttcatt
     1381 ttctttcagt aactgctctt caaactcttg cgctcggcct agctcatttt tgttcaagaa
     1441 ataattctgt gagacgcgat cgatttcaag caacacgtaa cggttgctct gcaggacatg
     1501 atccattttc ttttgcaggt tcgcagtgtg acgacctaca aattctttgg cttccatttc
     1561 tttttccatg atagcataga ctgcttcgat gtcgcgttct accttgtcca tcttcttgcc
     1621 tgcttcattg atatcggcaa ggccgatgga atctttcgct tcttggattt ctttttcaac
     1681 cgcagctatc tcagctgaga catctacacc ttcgaaaatg tatttttcat ccaacaggcg
     1741 ttggtagccg tctttcagat cactcacttg ttcttcgtac tcggtcttga ttttttcgtt
     1801 caattcaggg atcttttcga caacttcttc catcaccaac aaatcttgct cgatacgcga
     1861 caagatctct ttggcttcca tatggtcgcc ctcgttcgtc aatgaattga atttcgtgaa
     1921 atccaactcc atgtaattca ggttcttctc taacgtttcg attgccggac cgaatgtaaa
     1981 gctgtgcgcc agtaattgct tgcggatttt attgtatcgt tcctggattt cttccaactc
     2041 tttgcggttt tcttgcgcac tttccaatag cttttcgaga gccttgttca ctttttcgac
     2101 gctgttcttc gtttcgtcga tcaattgatc cgcttgcgag atggcttgct tcgccttaat
     2161 gaaattcatg cgttgaatgt actgttcggc actgaccagc gccgcctcaa tttcaggata
     2221 ctgaaaacgc gtaatggtct gccacgtggc ttgccaactc tcataggtgc gcttcgtttg
     2281 ccctgtcaaa ttcatgtttt tcaacgtgaa cagattgtcg gccaccggta tggccatcgc
     2341 tttttgtttc ttctcatcga ttgctttgat ccgattcgct tgttgtcttg tcaaatacat
     2401 gatcgcacca tacccgacca atactaacaa tattatcgct actaaccaat atataaactc
     2461 catcaataaa tcctccacct attccgacaa tttcgtttat ttgatttaaa attgaaaacg
     2521 cccaatatat ctcatttttt agtataacat agaaagaacc aatcataaac cagaaaataa
     2581 tctggaaact taattccttc gtttggaata ccccaagtgg tccaccgctg tgcaaacgat
     2641 gaatatcatc actttttcat tgcatttact gattttgttt ggtacaataa cctttgtgta
     2701 aaataatgca gcgagaaaga agcacccgcg tcaacagttc atcgccatct gtagatggtt
     2761 caattgcgta cccttgcggc tgcataaggt gaagattgaa ggatgacctg cacgcattgc
     2821 tgacttatca ttcttatttt actccaaaaa tcaaacattt attggaggaa ttttattatg
     2881 tcacgttata caggaccaag ttggaaacaa gctcgtcgtc taggcatctc ccttttggga
     2941 acaggtaaag aattggagcg tcgtccatac gttccaggtc aacacggccc taacaaccgt
     3001 aaaaaattat ctgaatatgc tatgcaattg caagaaaaac aaaaattgcg tcacatgtac
     3061 ggcatgaacg aacgccaatt cgcaacttta ttcaaaaaag ctggtaaaat caaagaaggt
     3121 aaacaaggtg ttaacttcat gatcttattg gaacaacgtt tggataacgt agtttaccgt
     3181 ttaggtttgg cttctactcg tcgtcaagca cgtcaattag ttaaccacgg tcacgtaact
     3241 gtcgatggca aacgtgtcga tatcccttca tacgaagtgg ctgttggcca agtaatcggc
     3301 ttgcgtgaaa aatccaaaaa cttggtagtt gttaaagcag ctgctgaagc tttgttcgga
     3361 cgtcctgact tcattacttt cgacgaagaa aaattggaag gttcattgaa ccgtctgcca
     3421 gttcgcgaag aattgccagc tgaaatcgat gagtcattcg tagtagaata ctacaacaaa
     3481 ttaggctaat ctccccggag agcggcatca aggaaacggc aaagcaattt gccgtttttt
     3541 ttgtacagga aaagcggaac gggttcgttc agcccttctt taaggaaaca ctgtgaccta
     3601 atcggtcaag tactctgatg tcacagtgcg acat
//
LOCUS       DAOMUJ010000151         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7263_length_3621_cov_1.939428, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000151 DAOMUJ010000000
VERSION     DAOMUJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7263_length_3621_cov_1.939428"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..790
                     /locus_tag="PLT73_04890"
     CDS             14..790
                     /locus_tag="PLT73_04890"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRM19749.1"
                     /translation="MQRYIIPPLTRDYQTKPIILSDDFHHHMVHVMRMKQGEQVYLAD
                     NSGISFVAELIDISANTVSLKWVADEDRSTELPVKITIACGLPKGDKLEYIVQKGTEL
                     GAAAFLPFAAKNAVVKWTADKSAKKQQRLQKIAREAAEQSHRQKEPFVHPVHNLKELL
                     AEAEKYSKVLIAYEEDAKVGEGSILVNTLGNLEPGDSLLFVFGPEGGLAPEELAAFRQ
                     AGHKSCALGPRILRAETAPLYALAAVSYEMELRKGADKNG"
     gene            783..1448
                     /gene="deoC"
                     /locus_tag="PLT73_04895"
     CDS             783..1448
                     /gene="deoC"
                     /locus_tag="PLT73_04895"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM19750.1"
                     /translation="MVEKLNKYIDHTLLRQDATEEEIKALCEEAREYDFKSVCVQPYW
                     VKKVEAFLTGSDVLVCTVVGFPHGANTAEVKTFEAKQAVQNGADEVDMVINIGALKDG
                     DYQTVEHEIAALVEAVKGKAILKVIIETCLLTDDEKVIACQLAQKAGADFVKTSTGFS
                     TGGATLEDIALMRKTVGPVMDVKASGGVRTYEDALAFIAAGATRLGTSSGKKIVEGWL
                     AVR"
     gene            1725..>3621
                     /locus_tag="PLT73_04900"
     CDS             1725..>3621
                     /locus_tag="PLT73_04900"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRM19751.1"
                     /translation="MPKIKTYSPEEVVQLCAEYMNETHVAFVQKACDFAREAHSGQMR
                     KSGEEYFVHPTQVAAILADLKLDPETIATGFLHDVVEDTEFTLDDIEREFSKTVAILV
                     DGVTKLGKIEYKSHEEQQAENHRKMLMAMAKDLRIIMVKLADRLHNMRTLKYHKPEKQ
                     RTISNETLEIYAPLAHRLGMNRIKWELEDTSLRYLNPQQYYRIVHLMNSKRDERELYI
                     KDTINKIKDSIDELEIEADIYGRPKHINSIYRKMKDQKKQFTEIYDLLAIRVIVHSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGTQGTPVEIQIRTIEMHQ
                     VAEYGVAAHWAYKEGITKKIDPDKMPHQIDWFRDLIDLQDDSKNASEFVESVKEDIFK
                     DKVYVFTPKGDVVELPSGAGPLDFAYNIHTEIGHKTIGAKVNGKIVPLNYKLQNGDII
                     EVMTSPNSFGPSRDWVNLVSTSKAKNKIKRYFKLQDRDEHVIKGRDMLEKQLTEMEFS
                     PKEFLTKQAIKDLLNRFNVNSEDDLYAAIGFGELSAFIVSNRLTEKARRERDKHKAEQ
                     QLDTIEQKTKKDNPKMKIRHEGGVVIQGVDNLLIRISHCCNPVPGDEIVGYITKGRGI
                     SVHRKDCP"
BASE COUNT         1092 a          761 c          938 g          830 t
ORIGIN      
        1 ggaggattga agcatgcaac gatacatcat tccgccgctg acaagagatt atcaaacgaa
       61 gccgatcatt ttgtcggatg atttccatca ccatatggtg catgtgatgc gcatgaagca
      121 gggggagcaa gtatatttgg ccgacaattc aggcatttcg ttcgttgcgg agctcattga
      181 catatccgca aatacggtca gtttgaaatg ggtggctgat gaagaccgtt caacggaact
      241 tccggtcaag attacgattg cctgcggttt gccgaagggc gacaagctgg aatatatcgt
      301 acagaagggt acagagttgg gcgcggcagc cttcctgcct ttcgcagcaa aaaatgcggt
      361 tgtgaagtgg acagcggaca aaagcgcaaa aaaacagcag cgcctgcaga aaattgcgag
      421 agaggccgcg gagcaatcgc accgccaaaa agagcctttt gtgcatcctg tgcacaacct
      481 caaagagttg ttggcggaag cggaaaaata cagtaaagta ttgattgcct atgaagagga
      541 tgcaaaggtc ggtgagggca gcatcttggt aaacacactg ggaaatcttg aaccaggcga
      601 ttctttgttg tttgtatttg gtcctgaagg cggattagcg ccggaagaac tggcggcgtt
      661 cagacaagcc ggtcataaga gctgtgcttt gggaccgagg attttgcggg ccgaaacagc
      721 ccctttatat gcgttggcgg cagtatccta tgaaatggaa ttaagaaaag gagcggataa
      781 aaatggttga aaaattaaac aagtatatcg atcacacatt attaagacaa gatgcgacag
      841 aggaggaaat caaagccctt tgcgaagagg cgagggaata tgacttcaag tccgtctgcg
      901 tgcaacccta ctgggtcaaa aaggtggaag ctttcctgac tggctcggat gtgcttgtct
      961 gtacggtcgt tgggtttccg catggtgcca atacggctga agtgaagact ttcgaagcga
     1021 aacaggcggt ccagaacggt gctgatgaag tcgatatggt catcaacatt ggcgctttga
     1081 aggatggcga ttatcagact gtcgaacatg aaatcgcagc tttggtcgaa gccgtaaaag
     1141 gcaaagcgat cctgaaggtc atcattgaga cctgtctgtt gaccgatgat gaaaaagtta
     1201 tcgcttgcca gttggcccaa aaggctggtg ccgactttgt caagacatcc accggctttt
     1261 caacaggtgg tgcaactttg gaagatatcg cactgatgcg taaaactgtc ggtcccgtga
     1321 tggatgtgaa agcaagcggt ggagtgcgca cgtacgaaga cgccttggct ttcattgcag
     1381 ccggcgcgac tcgtttgggc acttccagtg gcaagaaaat cgtcgaaggt tggcttgcgg
     1441 tcagataaat gcatggggtg ctccgtttgc atccgaataa ttaaatgatc aaggaacctt
     1501 gaatgactgc tttttttatg aagctgtcct tcaaggtttt ttatagtgca taaagctcgt
     1561 gaaccgagga taattgttga tggcgcatcg ttccgattta aagtataata aaaggaaata
     1621 ttcatttttt tatttacgga acagcgtatg ttcggtattg gttccgtctg agaatggaac
     1681 tgcagtaagg aaatacataa tcttaaagag tcaggagtga caacatgccg aaaatcaaaa
     1741 cctattcacc tgaagaggtt gtgcagctat gcgcggaata tatgaatgaa acccacgtgg
     1801 catttgtcca aaaagcgtgt gattttgccc gggaggctca tagcggacag atgcggaagt
     1861 ccggtgagga atacttcgtt catccgactc aagtcgcagc gatattggca gatttgaaat
     1921 tagatccaga aacgatcgca acgggatttt tgcatgatgt tgtggaagat acagaattta
     1981 ctttggatga tatcgaacgg gaattctcca aaaccgtcgc cattctggtg gatggcgtta
     2041 ccaagttagg gaaaatcgaa tacaagtcgc atgaagagca acaggctgaa aaccaccgca
     2101 aaatgttgat ggccatggcg aaggatttac ggatcatcat ggtgaaacta gcggacagat
     2161 tgcacaatat gcggacattg aaatatcaca agccggaaaa acaacggacc atctcaaatg
     2221 agactttgga gatatatgca cccttggccc accgcctggg gatgaaccgg atcaaatggg
     2281 aattggagga tacgtcgctc aggtatttga atccacagca atattaccgg attgttcact
     2341 tgatgaacag caaacgcgat gaacgggagc tctacatcaa ggacacgatc aacaaaatca
     2401 aagactcaat cgatgaatta gagattgagg ccgacatcta cggcaggccg aagcatatca
     2461 actccattta tcggaaaatg aaggaccaaa agaaacaatt cacggaaatc tatgatcttt
     2521 tggctatccg cgtcatcgtc cattccatca aagactgtta tgccgttttg ggtgctattc
     2581 atacaaaatg gaaaccgatg ccgggtcggt tcaaagacta tatcgccatg cctaaggcga
     2641 atatgtatca gtcgctgcat acgacagtta tcgggacaca aggaacgcca gtcgaaatcc
     2701 aaatccggac aatcgaaatg caccaagtcg cggagtacgg ggttgcggct cactgggcat
     2761 acaaagaggg tatcaccaaa aaaatcgatc ccgataagat gccccatcag atcgattggt
     2821 tccgtgatct gatcgacttg caggatgatt ccaagaacgc tagtgagttc gtcgaaagcg
     2881 tcaaggagga tatcttcaag gacaaggttt atgtcttcac tccgaaaggc gatgtcgtcg
     2941 aattgccatc gggtgccggc cctttggatt tcgcctataa tatccatact gaaatcggac
     3001 ataaaaccat tggggcaaaa gtcaatggca agattgtccc tttgaactac aaactgcaga
     3061 atggcgatat catcgaagtg atgacatccc cgaactcatt cggtccgagc agggactggg
     3121 tcaacttggt ttctaccagt aaggcaaaaa acaaaatcaa acgctacttc aagctgcagg
     3181 accgtgatga acacgtcatc aaaggccgcg atatgctgga aaaacaactg acggaaatgg
     3241 aattttcacc aaaagaattc ctgacgaaac aagcaatcaa agatttattg aatcgcttca
     3301 atgtcaattc agaggatgat ctgtatgcag ccatcgggtt cggtgaattg tccgcgttta
     3361 ttgtatccaa ccgtttgacc gagaaagcac gccgggagcg ggacaaacac aaagcggaac
     3421 aacagttgga cacaatcgag caaaaaacga aaaaagacaa ccctaaaatg aagatccgcc
     3481 acgagggcgg tgtagtcatt caaggtgtag ataatctgct catccgcatc agccactgct
     3541 gcaatccggt cccaggagat gaaattgtcg gatacatcac aaaagggcgc ggcatctcag
     3601 tccacaggaa agattgcccg a
//
LOCUS       DAOMUJ010000152         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7415_length_3564_cov_3.286976, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000152 DAOMUJ010000000
VERSION     DAOMUJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7415_length_3564_cov_3.286976"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1066)
                     /gene="argC"
                     /locus_tag="PLT73_04905"
     CDS             complement(26..1066)
                     /gene="argC"
                     /locus_tag="PLT73_04905"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRM19752.1"
                     /translation="MIHVSIIGATGYTGVELVRLLGLREDVVLDHLTSRTYAGQKIQH
                     LFPHLLGNIDHTCEELDLEQVTTDSDIIFVALPHGHALPIAKKAKEKGKKVIDLGADF
                     RLKDAAVYEEWYKVAHTEPELLEEAVYGLPEWDRDAIKDATVIANPGCFVTSILLGLM
                     PLMKKGWVVPNSIVCDSKSGTSGAGRAGNVANLFTEVESSFKAYGVANHRHTPEIEQL
                     LTQMAGTDTMIQFTPHLVPMSRGILSTIYATVTGGHTAEEIHALFEEAYADEPFVQVL
                     PAGMWPMTKSVKGTNNCQLGVTYDERTGRVIIVSVIDNLMKGAAGQAVQNMNLMFGLA
                     ETTGLQMIGLFP"
     misc_binding    complement(1180..1406)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1467..2483)
                     /gene="argF"
                     /locus_tag="PLT73_04910"
     CDS             complement(1467..2483)
                     /gene="argF"
                     /locus_tag="PLT73_04910"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRM19753.1"
                     /translation="MLEQVFQGRSLLAEKDFTREELEYLIDFSAHLKDLKKRNIPHRY
                     LEGKNIALLFEKASTRTRAAFTVAAIDLGAHPEYLGKNDIQLGKKESVEDTAKVLGSM
                     FDGIEFRGFSQKTVEDLAKYAGVPVWNGLTDEWHPTQMIADFLTVKEHFGKLAGLKLV
                     YVGDGRNNVANSLLVTGAILGVHVTICAPESLFPNEELVEMAQKFAEKSGSKIEITAD
                     VDKGVKAADVLYTDVWVSMGEEDKFAERIELLAPYQINMEMIEKTGNDDVIMLHCLPA
                     FHDTKTQYGQEIAETFGMREMEVTDAAFRSKHGRQFEQAENRMHSIKAIMAATLGNLF
                     IPKV"
     regulatory      2735..2850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3049..>3564
                     /gene="ribD"
                     /locus_tag="PLT73_04915"
     CDS             3049..>3564
                     /gene="ribD"
                     /locus_tag="PLT73_04915"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRM19754.1"
                     /translation="MQDKDTYFMQMALGLAEKGRGAVAPNPMVGAIIVKDGRIIGSGY
                     HEKIGEGHAEVNAFRSATEDVTGATIYVTLEPCSHFGKTPPCSDKIIEKKIGRVVIAA
                     LDPNPLVSGRGVKKLQAAGIEVITGVLAEESSRLNEIFMKYIVKKEPFVVMKAAMSLD
                     GKIATRTGESQW"
BASE COUNT          867 a          921 c          793 g          983 t
ORIGIN      
        1 catttcctcc ttcagtcttc cgttattacg ggaaaagccc tatcatctgt aatcctgtcg
       61 tttcggcaag tccaaacatc aggttcatat tttgcacggc ttggccggca gcgcccttca
      121 tcaagttgtc gatgaccgac acgatgatga cccggcctgt acgctcgtcg taagtgacac
      181 ccaattggca gttattcgtg ccttttaccg atttcgtcat cggccacatg cctgcaggca
      241 gaacctgcac aaacggttcg tcggcgtaag cttcctcaaa aagagcgtgg atttcttctg
      301 ccgtatgccc cccggtgacc gtcgcataga tggtgctgag gatgcctcgg ctcatcggca
      361 ccagatgcgg tgtaaattgg atcattgtgt ccgttccggc catctgcgtc aacagctgct
      421 cgatttccgg tgtgtggcgg tgattcgcta ccccgtaggc tttaaagctg ctctccacct
      481 cagtgaagag gttcgctacg ttgccggcgc ggcccgctcc ggatgtgccg gatttggaat
      541 cgcagacgat gctgttcggc acgacccagc cttttttcat taatggcatc aagcccaaga
      601 ggatgcttgt cacaaaacaa cccggattgg cgatgactgt tgcatccttg atcgcgtcgc
      661 ggtcccattc gggcagaccg tagacagctt cttccaaaag ttccggctcc gtgtgcgcga
      721 ctttgtacca ttcttcatag accgccgcat ccttcaatcg gaaatcggct cccagatcga
      781 tgactttttt acctttttct ttagctttct tggctatcgg caacgcgtgt ccatgcggca
      841 aagccacaaa aataatatcg ctatccgtcg tcacttgttc cagatccaat tcttcgcaag
      901 tgtgatcaat gttgcccaac aggtgcggaa aaagatgctg gatcttttga cccgcatacg
      961 tacgggaggt cagatgatcc aagaccacat cctcgcgcaa tcccaataat cggactaatt
     1021 ccacccccgt gtaacctgtt gctccaatga tactgacatg aatcatactt cccatcctcc
     1081 tcattaatgt ttgtagtaag tgcaggctaa tcctgtttac aaattgttgt gactatacaa
     1141 aaaaaacttc cgtgcctata ccaaatcaaa tagattcagt atagggacga aagttcgtgg
     1201 tgccaccctt attttccaac atgcgcgcat gctggactca agtattgcta cttttgcgat
     1261 aacggggcaa ccggattaaa ctttatggtt cacattcagc ttatcaactc aaagttcctg
     1321 ttcatccacc gcggttgacc ttgctttcac cctccaaggt tcgcttgact tcgcaaatgg
     1381 attactcttc ttgtcaaagt ttttgtttgt gttagatgtt gttttacatt ttcattaaat
     1441 ttatcatctg tttttcactt tgtcaatcaa acttttggaa taaataaatt tcctaaggtc
     1501 gcagccatga ttgccttgat ggaatgcatg cggttttcgg cctgctcaaa ctgacgtccg
     1561 tgcttgctgc ggaaggcggc atcggtcact tccatctcgc gcatgccgaa tgtttccgcg
     1621 atttcctggc catattgcgt ttttgtatca tggaaagccg gcaaacagtg cagcatgatc
     1681 acatcgtcat ttccggtctt ctcaatcatc tccatattga tctgataagg cgccaacaat
     1741 tcgatacgtt ctgcaaattt atcttcttca cccatcgata cccatacatc ggtgtaaagg
     1801 acatcagccg cttttacgcc tttgtccaca tcagccgtga tttcgatttt gctgccgctc
     1861 ttttcagcga atttctgggc catctcaacc aattcctcgt tcgggaacag cgattccggc
     1921 gcgcagatcg tgacatgcac accgaggata gcgccggtaa ccaacaggct gttggcgaca
     1981 ttgttgcgtc cgtccccgac atagaccaat ttaagtccgg ctaattttcc gaaatgctct
     2041 ttgaccgtca ggaaatcggc gatcatttgg gtcggatgcc actcatccgt caagccgttc
     2101 cataccggta caccggcata tttcgcaaga tcttcgactg ttttttggct aaagccacgg
     2161 aactcgatac cgtcgaacat gctgccgagt actttagcgg tatcttcaac ggattccttt
     2221 ttgcccaatt gaatgtcatt tttcccgaga tattccggat gcgccccaag atcgatggca
     2281 gccactgtaa aagcagctct agtacgagtg gaggcttttt cgaacaaaag ggcaatgttt
     2341 ttgccttcca gataacggtg cggaatattt cgtttcttca gatccttcaa atgcgccgaa
     2401 aaatcaatca gatactccaa ttcttcccga gtgaaatctt tttccgccaa taagcttctt
     2461 ccttgaaata cttgttccaa aattccgcca tcccctttta atgttattct cactgtcgct
     2521 cttgcgaacg atcatttccg agtcatgatg caactattat tggactcatt ttatacccaa
     2581 ttctaatgga ttacaaactt tattttaatc taacggttat aatccgacca aatacgggca
     2641 gcaggagaca ctcttccact acggaccatt tgtttgcaaa aatttttatt tgacaatgat
     2701 acggattggg attatcctag agacaactta attgaatagt cttcagggac gggtgaaaat
     2761 ccctaccggt ggtaaagccc acgagccgtt aaggcagatt cggttaaatt ccgaagccga
     2821 cagtcagagt ctggatgaga gaagacagaa accattttcg agtgggcata cgtttatttg
     2881 ccatgcaaat aacacttgtt cgccattcgt tcatgaatcg gaatgtcaat ccctgggtgt
     2941 agtgtatacc cagggatttt ttggccttat ttagctggcc ttcctttgtc gagccctgaa
     3001 gcagaaatgt tcagggcttt ttattttgga aatgggaggc acacaaagat gcaagataag
     3061 gatacatact ttatgcaaat ggcgctggga cttgccgaga aaggccgcgg tgccgttgcc
     3121 ccgaatccga tggtcggcgc catcatcgtc aaggacgggc gcatcatcgg gtccggctac
     3181 cacgaaaaaa tcggggaagg ccatgctgaa gtgaacgcgt tccgctcggc aacagaagat
     3241 gtgaccggcg caaccatcta tgtgactctg gaaccctgct cccatttcgg caaaacaccg
     3301 ccctgctccg acaaaatcat cgaaaagaaa atcggtcgag ttgtcatcgc ggctttggat
     3361 ccgaatccgt tggtttccgg acgcggcgtc aagaaactgc aggctgccgg catcgaagta
     3421 ataaccggcg tcctggctga agaaagcagc cgtttgaatg aaatattcat gaaatacatc
     3481 gtaaaaaaag aacccttcgt cgtcatgaaa gcagcgatgt ccctggacgg aaagatcgcg
     3541 acccgaaccg gcgaatcgca atgg
//
LOCUS       DAOMUJ010000153         3520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7542_length_3520_cov_2.330447, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000153 DAOMUJ010000000
VERSION     DAOMUJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7542_length_3520_cov_2.330447"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    297..306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            347..1678
                     /gene="murC"
                     /locus_tag="PLT73_04920"
     CDS             347..1678
                     /gene="murC"
                     /locus_tag="PLT73_04920"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRM19755.1"
                     /translation="METETIYHFVGIKGSGMSALALILHGKGYRVQGSDVETYFFTQK
                     GLDDASITIMPFNEANITPGLTVIAGNAFPDSHEEIAKAKELGLTVIRYHDFIGELIK
                     NYTSVAITGSHGKTSTTGLLSHVLDGIAPTSYLIGDGTGFGREDAEYFVLEACEYRRH
                     FLAYSPDYAIITNIDFDHPDYYQDINDVYSAFAAFASQVKKGIIACGEDAYVRELKGK
                     YPVTFYGFAEDNDVVAKNVTKNTNGSQFDVEINGELFGHFEIPSFGTHNILNSLAILT
                     FCWLEGLDKDKVAAQLKTFSGVKRRFSEKYISDMVVIDDYAHHPSEIRATLDAARQKY
                     PEKEIIAIFQPHTFTRTVALLSEFAEALELADKVFLCDIFGSAREKDGQVSIGDLAEK
                     IEKGATVLNVNNMSPLLQFHDAVAIFMGAGDVQKFELAYEELLSHSVPTKN"
     gene            1846..2397
                     /locus_tag="PLT73_04925"
     CDS             1846..2397
                     /locus_tag="PLT73_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRM19756.1"
                     /translation="MKMGLKDIKLGKSIDEIQEGDSLTVTEIIEDKDILLYLGLTNDG
                     NPLYIQHDYSQTTRFHKPIVPTVLLVGILTSNVSKHLPGPGSHIVDVSLNVIEPIYHN
                     NMITFNFEVERVDERREQVTISVVGTNMENERVLDAELIVETPRKLIFDEAENVIMNE
                     RTEVEENADKVVYPTTMADDDSE"
     gene            2419..>3520
                     /locus_tag="PLT73_04930"
     CDS             2419..>3520
                     /locus_tag="PLT73_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRM19757.1"
                     /translation="MTDKKKKLLLVDGSSVAFRSFFGLPNLDGFRNKNGLHTNALYAF
                     NNILERIFQTEEPTHLLVAFDSGKTTFRNALYGDYKGGRASMPSELSEQWPYFGVLVH
                     AFGGKTYELVNYEADDIIGTYSRMADQAGYDVVIISGDKDMIQLTTDNIRVDITVKGV
                     SELKSYTPDVIREEYGIEPRQIIDMKGLMGDSSDNYPGVTKVGEKTALKLLTEYGSIE
                     NLYEHIDELKKSKMKENLINDKENAFMSKTLATIDLDSPVTLKLDELAVEGKQIDKLT
                     DFYREMDFNGFLDKLGVASEDRSESFEDIHFEVLTEIRPEHFKDRMALYVEMLEDNYH
                     DGKILAVSWGTDEKIYTASLETVLASVVFMEWA"
BASE COUNT         1001 a          727 c          878 g          904 t
ORIGIN      
        1 cgatcgttgt tgggtatcat ggagcaggaa cgctcttacg atgcagccgg gaaatttgtc
       61 catgaggcag ctgattcggc aagtcactct tatttttagt catggcattg taacattaac
      121 ttgatttgta tgctgcggtt ggtggcataa tgaaattatt gacatgtttg acatcaaatt
      181 ttaaataatt tcatgcattt tcttaattcg tgcgtgctat actggttccg gttcaataaa
      241 tggacagcaa gtcatcatgt cctaagagag tgttttgagg cacaaacatc agaaggnnnn
      301 nnnnnnagcc gaaagactaa ccttccttat tcatttggag gtaattatgg aaacggaaac
      361 gatttatcat tttgtcggca tcaaaggttc cgggatgagt gcgttggcat tgatcctgca
      421 tggaaaaggc tatagagtcc aaggttccga tgtcgaaact tatttcttta cgcaaaaagg
      481 tttggatgat gcaagcatca caatcatgcc attcaacgaa gcgaatatca caccgggtct
      541 gacggtcatc gcagggaatg ctttccctga ttcccatgag gaaatcgcta aagcgaaaga
      601 acttggtttg acggttatcc gctaccatga tttcataggc gaattgatca aaaactatac
      661 gagcgtcgcc ataaccggtt ctcacggaaa aacaagcaca accggtttgt tgtcccatgt
      721 gttggacggg attgctccga ctagctattt gatcggtgac ggcactggct tcggccggga
      781 agatgcggag tactttgtgt tggaagcttg cgagtacaga cgtcatttct tggcctattc
      841 accggactac gccatcatca cgaacatcga tttcgatcac ccggattact accaggatat
      901 caatgatgtt tacagcgcgt ttgctgcgtt tgcatcccaa gtgaaaaaag gcatcatcgc
      961 ttgtggggag gatgcctatg tccgtgaact gaaaggtaaa tatccggtaa cgttttacgg
     1021 gttcgctgaa gacaatgatg ttgtcgccaa aaatgtcacg aaaaacacaa acggcagtca
     1081 gttcgatgtg gaaataaatg gcgagttgtt cggtcatttt gaaatcccgt cattcggcac
     1141 gcacaacatc ctcaactcat tggccatcct cacattctgc tggttggaag gattggacaa
     1201 agacaaggtt gctgctcagt tgaagacctt ctcaggcgta aaacgtcgct tcagcgaaaa
     1261 atatatttcg gatatggtcg tcatcgatga ttatgcccat catccatccg aaatccgcgc
     1321 tacactcgat gcagctagac aaaaataccc tgaaaaggaa atcattgcga ttttccaacc
     1381 gcataccttc acgcgcacgg ttgctctgct tagtgagttc gccgaggcgc tggaattggc
     1441 cgacaaggtc ttcctttgcg atatcttcgg ctccgctcgt gaaaaggacg gacaggtttc
     1501 gatcggcgat ttggccgaaa aaatcgaaaa aggcgcaacg gtgttgaacg tcaacaacat
     1561 gtctccgctt ttgcagtttc atgatgctgt cgccattttc atgggtgcag gggatgttca
     1621 gaagtttgaa ttggcttacg aagaactgtt gagccatagt gtgccgacaa aaaattaatt
     1681 caatgcatca aaaaagtcca ggaagcaatt cttgggcttt ttttgatgca agacagagcg
     1741 ggcttggtcc ggttatctga ataaataggc ccaaacggtt aaacggcttt tatcggaacg
     1801 gggtttttga tatgatagac atatatgtgt tcaaagaaag gaaaagtgaa aatgggtctg
     1861 aaagatataa agcttgggaa atcgattgat gaaattcaag agggggattc gttgactgtc
     1921 accgaaatca tcgaagacaa agatattttg ctgtatcttg ggttgacgaa tgacgggaac
     1981 ccgctctata ttcaacatga ttattcgcag acgactcggt ttcataaacc gatcgtgcca
     2041 accgtattgt tggtggggat tttgacgagc aacgtttcca aacatttacc gggtccgggt
     2101 tcacatattg tggacgtttc tttgaatgtg atcgaaccga tctaccataa caacatgata
     2161 accttcaact ttgaagtgga gcgcgtggac gagcgccgtg aacaagtcac aatcagcgtt
     2221 gtcggaacga atatggaaaa tgaacgagta ttggatgctg aattgatcgt agagacaccc
     2281 cgcaaattga tttttgatga ggcagagaac gtgatcatga acgagcgaac tgaagtcgag
     2341 gagaatgccg ataaggtagt ttatcctaca acgatggcag atgacgattc agaataagca
     2401 gagttaggat gttaacagat gactgataaa aagaaaaaat tactattggt ggatggcagc
     2461 agtgtggctt tcagatcctt ttttggattg ccgaatctgg atgggttccg caacaaaaac
     2521 ggcttgcata cgaacgcgct ctacgccttc aataatatat tggaacgcat cttccagaca
     2581 gaagagccga cgcatctgtt ggtagcattc gattcaggaa aaacgacttt ccgcaatgcc
     2641 ctttatggcg actacaaagg cgggcgtgca agcatgccgt cggagttatc cgaacagtgg
     2701 ccctattttg gcgtccttgt ccatgccttt ggtggcaaga cgtatgaatt ggtcaattat
     2761 gaagctgacg atatcatcgg gacttactcg cggatggccg atcaagccgg ctatgatgtc
     2821 gtcatcattt caggcgataa ggatatgatc caattgacga cggataatat ccgggtcgat
     2881 atcaccgtca aaggcgtgtc ggaattgaaa tcctacaccc cggacgtcat ccgtgaagaa
     2941 tacggcatcg aaccgagaca gatcatcgac atgaaaggtc tgatgggcga ctcgtcggat
     3001 aattatccgg gcgtcactaa agtaggcgag aagaccgctt tgaaactctt gaccgaatac
     3061 ggttccatcg aaaatctata cgagcatatc gatgaactga agaaaagcaa gatgaaagag
     3121 aaccttatca atgataaaga gaatgcgttc atgagcaaaa ctttggctac aatcgatctg
     3181 gactcgccgg tgacattgaa gcttgatgaa ctggcggttg aaggcaaaca gatcgacaaa
     3241 ctgaccgact tttaccgcga aatggatttc aatggcttct tggacaaact gggcgtggcc
     3301 tcggaagacc gctcggaatc cttcgaggac atccatttcg aagtcctgac cgagatccgc
     3361 cccgagcatt ttaaggaccg gatggcactg tatgtagaga tgcttgaaga caactaccat
     3421 gacgggaaaa tcctcgcagt cagctgggga acggatgaaa agatttacac agcttcattg
     3481 gaaacagtgc tggcaagtgt tgttttcatg gaatgggctg
//
LOCUS       DAOMUJ010000154         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7564_length_3513_cov_2.054945, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000154 DAOMUJ010000000
VERSION     DAOMUJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_3513_cov_2.054945"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLT73_04935"
     CDS             <1..242
                     /locus_tag="PLT73_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM19758.1"
                     /translation="VKTNQRGKHIGENLVMHSLEMAIESGFRIMQFNAVVANNKGAIS
                     LYKKLGFKELGTIPGGFKMDDGTFVDIIPFYLELS"
     gene            287..664
                     /locus_tag="PLT73_04940"
     CDS             287..664
                     /locus_tag="PLT73_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM19759.1"
                     /translation="MNISEAVKITGLTAATLRYYESEGLIPPVNRKNGGVRDYQKDDL
                     GWIDFIKCMRSAGMSVESLIEYTTLFRNGTESDEARNNILIRERDQLLVKQKEINETI
                     ERLNHKIQSFGSKLKESEKSLPH"
     gene            complement(850..1203)
                     /locus_tag="PLT73_04945"
     CDS             complement(850..1203)
                     /locus_tag="PLT73_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM19760.1"
                     /translation="MNISEAAEKSGLTAVTLRYYERIGLIPPVTRGNGGVRLYKESDL
                     GWIDFIKCMRNAGLSIESLIEYTTLFDQGKDTVVARKEILIEEQKKMEERYNELGKTL
                     EKLNKKIEHYEQDHS"
     assembly_gap    1413..1422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1629..1639
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1640..>3513)
                     /locus_tag="PLT73_04950"
     CDS             complement(<1640..>3513)
                     /locus_tag="PLT73_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HRM19761.1"
                     /translation="AVGLQTYLDGVGQLDAGALALNEGVGTLTAAMPDLEAGVSRLTD
                     GAVSAAAGSTSLSNGLSSLATNSLSLSTGMNNLNNGMNQLAAGSAALTDGLTALNTNL
                     TSAEQQAKLAALQGGLDQIKIGIETLDSQLQQSALSGDLTAVLSQLNSLSTTLSSSQS
                     ELSTIIAAADPASNTAAIMAAITQSGTELTADQEAAVNQAISAQMAILANEQSSSIQT
                     ITNDLSALSSLNGIDASAITQQVTALQNSISGLSAGYATVYGGATTLVSGYGQIGATT
                     QALLNGGKSLQAAIGSAQSGSQQLADGLSQLSNGSEQLAQGGKSLSEGNTQVSAGLQT
                     LNEQTGPLAAGAEKLKDGTEALANGTTELVEKGSLLTDGISQLAAGAEKISDGSGQLA
                     DGSSQLNTGLISLLSGTNELGAKLSEGSAALNEVDATESTYAMMAEPVITNQIETAPV
                     ANNGTAMAPYMMSVALFVGAISLNLMYDIFTPRNYPKNGRSWWASKISVLSAVGICQS
                     LIMVTLLVKVNGLAPSSIDKTLLVTLLTAFVSVSIVMLFNLLFDKVGSFLMLIFLILQ
                     LSGSGGTYPIQLSNSFFEAIHPYLPMTYSIDAYRQLFGIGGSISMDLFILLGILVAFN
                     "
BASE COUNT          854 a          799 c          917 g          922 t
ORIGIN      
        1 ctgtcaaaac taatcaaaga gggaaacaca tcggcgaaaa ccttgtgatg cattcgctgg
       61 agatggcaat agagtcagga ttccgtatca tgcagttcaa tgctgtagtc gccaacaata
      121 aaggggcaat cagtctttat aaaaagctgg gtttcaaaga attggggact attccgggtg
      181 gttttaaaat ggatgatggc acctttgtgg atatcattcc gttttatttg gaactgagtt
      241 gattcattag ttgtatacgt tcaatgagag aaaggatgat tgaattatga atatatctga
      301 agcagtaaaa attactgggc tcacagctgc aacgctgcgt tattatgaaa gcgaagggct
      361 gattcctccc gtgaaccgaa aaaatggtgg agtccgtgat taccaaaaag acgatcttgg
      421 ttggatcgat ttcattaaat gcatgcgcag tgccgggatg tcggtcgagt ctctgatcga
      481 atatacgacc ttgttcagaa atggtacgga gtcggacgaa gcgcgtaata acattctgat
      541 tagggaaaga gatcaactgc ttgtcaaaca aaaggaaatt aatgagacaa tcgaaagatt
      601 gaatcataaa attcagagtt ttgggagtaa attgaaagag tccgaaaaga gcctgcccca
      661 ttaagttgat tgttacggtg gttgtagacc cggtaacaga aaagattgat ttccgataaa
      721 tgaaaaccat ccaagcgggc agttcgttgc ccgcttggat ggttttctca ttatgccagc
      781 aaaatcagcc tacatcaatg tacccagagc gcgaactgat cacggtgatg tcctcatcga
      841 cttttttgat tagctatgat cctgctcgta gtgttctatt ttcttgttca gtttttccag
      901 tgtttttcct agttcgttgt aacgttcttc catttttttc tgttcttcta tcaggatttc
      961 cttcctggca acgacggtat cttttccctg atcgaacaaa gttgtgtatt cgatcaagga
     1021 ttcgatcgat aaacctgcat tgcgcatgca cttgatgaaa tcgatccaac caagatcgga
     1081 ttctttatat aaccggacac caccgtttcc tcttgtaacc ggagggatca aaccaatccg
     1141 ttcgtaatag cgcagggtta cagctgttag tcctgatttt tctgctgctt ctgaaatatt
     1201 catttttctc acctctgctt agcataattt aatttatagg ccaagcttac tacttaaagt
     1261 taaggttaag tcaagaaaaa ttttcagatg gataaaaagt aaatggttct cagctccggt
     1321 gaagggctgc acaccggagt acgagggtaa cttttagccg caactccggc gaaggtctcc
     1381 tcatcggaga tggcaactca gaatattagc tgnnnnnnnn nnaacttcgg cgaagccgct
     1441 gttcaccgga gctggcgcaa cagcagcgtc cgccgtgctg cacgcacccg gaaaaggaca
     1501 aagaaaacac gcacagccta taccggtgtg cgtgttttgg cattcataga gtttttaagc
     1561 agtttgggtt tccatagagg ctgatttttc gtcgatcttg aaatcttgct tccgcaactg
     1621 tttccgttnn nnnnnnnnna ttgaaggcaa ctaggatccc cagcaggatg aatagatcca
     1681 tggagatgct gccgccgatg ccgaacagct ggcggtaggc atcgatcgaa tacgtcatcg
     1741 gcaggtaggg atggatcgcc tcgaagaagc tgttggacag ctggatcgga taagtgccgc
     1801 cggatcctga tagctgcagg atgaggaaaa tcagcatcag gaaggagccg actttatcaa
     1861 acaacagatt gaagagcatg acgatcgata ccgagacgaa cgcagtcaaa agggtgacca
     1921 acagcgtttt atcgatcgac gacggcgcca aaccgttcac cttcaccagt agtgtgacca
     1981 tgatgaggga ttggcagatg ccgacagcag aaaggactga gattttgctg gcccaccaac
     2041 tgcggccatt tttcggataa tttcgaggag tgaagatatc gtacatcaag ttcagactga
     2101 tggcacccac gaagagggca acggacatca tatagggtgc catcgcagtg ccgttgttgg
     2161 cgaccggagc cgtttcgatc tggttcgtga tgaccggttc agccatcatg gcataggtgc
     2221 tttccgtggc atcgacttca ttcagcgcag cgctgccttc cgacagtttg gcgccgagtt
     2281 cattcgtacc gctgagcaaa ctgatcaggc cggtattcag ctggcttgat ccatccgcca
     2341 attggccgga gccgtcactg attttttcag cgccggctgc aagttgactg ataccatcgg
     2401 tcagtagact gcctttttcg acaagctccg tcgtaccgtt ggcaagtgcc tctgttccgt
     2461 ccttgagttt ttcagcgcct gctgccaggg ggccggtctg ctcattcaga gtctgcaagc
     2521 ctgcacttac ttgggtattg ccttcgctca ggctctttcc accttgagcc aattgttcgg
     2581 aaccgttgct gagttgggac aggccgtcag caagttgttg ggagccgctt tgagcgcttc
     2641 cgatggcagc ctggagcgat ttgccaccgt tgagcagggc ttgggtggtg gcaccgatct
     2701 ggccatatcc ggaaacgagg gttgtcgcac cgccgtaaac agtggcatag cctgccgaca
     2761 gaccactgat gctgttttgg agtgcggtca cttgttgcgt gatcgctgag gcatcaatac
     2821 cgttcaaact ggaaagcgcg gagaggtcat ttgtgattgt ctgtatggaa ctggattgtt
     2881 cattcgcaag gatcgccatt tgcgctgaga tagcttggtt gacagccgct tcctgatcgg
     2941 ctgtcaattc ggttccgctt tgggtgattg cagccatgat tgcagctgta ttgctggcgg
     3001 gatccgcggc agcaatgatg gtgctcaatt ctgattggga cgacgaaagg gtggtgctga
     3061 gactattcag ctgggacaat acggcagtca agtctcctga tagtgcagat tgctgcagct
     3121 gactgtcgag tgtttcgatg ccgattttta tttggtcgag tccgccctgc agtgcagcaa
     3181 gtttagcttg ctgttcagca ctcgtgagat tcgtgttcag tgcggtcaag ccatcagtca
     3241 aggctgcgct gccagcagcc aattggttca tgccattatt caaattgttc atgcctgtcg
     3301 atagtgaaag gctgtttgtg gccaagctgg aaagaccatt gcttagcgat gtgctgccgg
     3361 cagccgcaga aaccgcgccg tcagtcagtc ggctgacacc ggcttccaga tccggcattg
     3421 cggcagtcaa ggtacccacg ccttcgttca atgccaaggc gcccgcatcc agttggccga
     3481 caccatcgag ataggtctgt aggccgacag cta
//
LOCUS       DAOMUJ010000155         3505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7588_length_3505_cov_2.133913, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000155 DAOMUJ010000000
VERSION     DAOMUJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_3505_cov_2.133913"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..2214
                     /locus_tag="PLT73_04955"
                     /pseudo
     CDS             303..2214
                     /locus_tag="PLT73_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722784.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
     assembly_gap    2215..2252
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2253..2479
                     /locus_tag="PLT73_04960"
     CDS             <2253..2479
                     /locus_tag="PLT73_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRM19762.1"
                     /translation="AGMRVKEGSLHFKPFVPKNWQGYDFKINFRGSLLDVQVIGGEVT
                     LTIEEGPELAVYLNDELVQVNEAVVVKTKH"
BASE COUNT         1009 a          697 c          895 g          866 t
ORIGIN      
        1 aatctgttgg atgttcagga gtacaaagtc aatgaaggtt tgctggaaac ccgcggcgtg
       61 ccgattttgt tcaagagtta cggggaagcg ctgctgatcg aggtctgaaa gtacatgaga
      121 aatattatag agtaatgcat ggttgcgctt gcgtggatgt ggccaaaatt gggataatgg
      181 agtcatcaga acaatcattt ttatgcgggc tccgaaaagg accggcatct gattccggaa
      241 agaaggactg ttatatgaaa agactctttg atatcgatcc ttggaaaata aagacggctg
      301 tattggacaa agaagagaag cggctgcagg aaagcttgac ttcaatcggc aatggctata
      361 tggggatgcg cggcaatttt gaggagggtt actcaggaga tacccatgaa gggacctact
      421 tggcgggtgt ctggtatccc gacaaaacgc gtgtgggctg gtggaagaac ggataccccg
      481 aatatttcgg gaaagtcatc aatgccatga acttcattgc cgtcggtatc ttcatcgatg
      541 gcgaaccgct tgatttggcg aaacagcctg tcgcggattt ccatatggaa ttggacatgc
      601 agcgcggcgt gttaaaccgt agcttccttt ggaaaagcga ggacgatgct gcggaaatca
      661 agttcacttt tgaacgcttc gtcagcatct cgcagaagga gctttgtctc gtgcgggtaa
      721 cggcagaagt gctgaaaggc tcggccgacc tggaattcca tccgcgtctg gatggcaacg
      781 tgacgaatga ggatgcgaat tacgaggaaa tgttctggga ggaagctggt cgcacgctgg
      841 atgcgcctgt cagtctgagc accaaaacga ttccgaatcc gttcgggatc gatcagttca
      901 cggtcactgc agcaatgagc aaccgcattt ccggactggg gcaaacgggc tgcaacgaaa
      961 gcggcctttt agtagaagaa actttttctg gactcgtttc cgctggcgaa aaggtcgttt
     1021 tggacaagcg tgtcgctatc gtcaccagcc gggacattcc ggatgccgag caggcgttca
     1081 tcgccaaaaa gatggcaact gaagctgctg ggaaaggcta cgacaggctt ctcgccgcgc
     1141 atgaacgtgc ttggaaagcg agatgggcga aagcggacgt gaaaataatg ggcaacgacg
     1201 aagcacagca agggatccgc ttcaatcttt tccaactttt ttctacttat tacggagaag
     1261 acgaacgctt gaacatcggt ccgaaaggct tcaccggcga gaaatacggc ggcgccacct
     1321 attgggatac ggaggcatac atggtgccga tgtacctttc cgtagccgat ccggaagtca
     1381 cccgccaatt gttgcgctac cgtcataagc agttgccggg tgctttccac aacgcacgcc
     1441 agcaggggct caaaggcgct ttgtatccga tggtgacctt caacggaatc gaatgccaca
     1501 acgaatggga aatcactttt gaggaaatcc accgcaacgg ggcgatcgcg catgccatct
     1561 acaactatac gtactacacg ggggatgaaa gctacttagt ggaaacggga atcgatgtcc
     1621 ttgtcggcat ttcccgtttc tgggcggatc gggtccattt ttccaaacgc aaccaaaaat
     1681 atatgatcca tggcgtgact ggcccgaacg agtacgaaaa caacgtcaac aacaattacc
     1741 ataccaacaa tatggcgacg tggacactcc agtacacttt ggacgcgcta aaaaaagtgt
     1801 cccccgagaa atgggcagag cagggcctgg cggaagcgga aacggaccac tggaaagata
     1861 ttgttgcccg gatgtattat ccgtatgatg aggaactggg ggtttttgtc cagcatgaca
     1921 cgttccttga taaagacctt cgtcctgccg atacgttgga tccgagcgaa aggcctttga
     1981 atcagcattg gtcatgggat aaaatcctgc gatccccgtt catcaaacag tccgatgtgc
     2041 tgcagagcat ctatttcctg aatgatcggt acagtatgga agaaaagaga cgcaactttg
     2101 atttctatga agcgatgact gtgcacgaat cttccttgtc cccatctgtg cacgctgtcc
     2161 tggctgcgga acttgggaag gaagaaaaag ctgttgaatt atatgcgcgc accgnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgccgga atgcgcgtga aagagggctc
     2281 cctgcatttc aaaccattcg ttcctaaaaa ttggcaaggc tatgatttca aaatcaattt
     2341 ccgcggcagc ctgcttgatg tgcaagtgat cggtggagaa gtgaccctca ctatagaaga
     2401 aggaccagag ttagctgtgt atttaaatga tgaattggtg caagtgaatg aagctgtcgt
     2461 cgtcaagacg aagcactgat cgaattgggg gctgctggaa ggcggccctt aattgcgttc
     2521 tatcattgct gaagagccgt actgtcatgg aaagtgtata ggcagttgaa tagaagctga
     2581 agacactaga attgtagcca taaagtcagc cggtaacttg ggggaataaa taaaaaaaat
     2641 tcaaataatt taaaaaaaag ggcaacatac acttgcaaag caagcgcttt catgttatta
     2701 ttacagtgta aagaaggaag ggtatataac tcgaaagggt tgtataaaat gaattcttta
     2761 catagaacca attgaagttc ttgcatctta tggttttaat ttcattcatg atcgagtaga
     2821 gactttaagt atagacccga aaaatctaga aaagattcaa catgacagat tgaatgaaaa
     2881 aagtaacgta gcagcagaaa agatttagat tagattgaaa aactatgaaa aattgattta
     2941 acctgtgaag ttaaaaccgt aatagaatgg ataaaggttc agaaatgaga aacattttag
     3001 gcgagtaaac ttaaaatgtt atctcggaga agattcgtat ttaaatgtga gtggcgagat
     3061 gccccgttta aatgttttat gaatcttcag tactacaact tcatattgtg gccatgttcc
     3121 tcattcagtg gaaaaagtag tccggaagtt tgtaagactc acatgaacga agatcatgtc
     3181 aacgaacagt aactttaaat accaaccgca acaatgttgg tagataaatt aacaggaact
     3241 agtgctacaa gcatgaagtg aggcaacctc gctatttgag gttgcctctt ttattttctt
     3301 gcgctacctg agcgtattca gccttatatt cgaaaaaaat gtttttcgct tcaaatgatg
     3361 tatccatagt aatgttgatg cactggcgtt gacaaggctt acaattttat ggtacagttt
     3421 agttcctcgg taatccgatg gatttttttg aaatctgttt gacatggatt tgaaatgatg
     3481 atagtattgt gaagttgtct tttgt
//
LOCUS       DAOMUJ010000156         3503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7592_length_3503_cov_2.770012, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000156 DAOMUJ010000000
VERSION     DAOMUJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7592_length_3503_cov_2.770012"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="argJ"
                     /locus_tag="PLT73_04965"
     CDS             <1..1046
                     /gene="argJ"
                     /locus_tag="PLT73_04965"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRM19763.1"
                     /translation="QALQQHDRFKAILINSGNANACTGKQGKADVEETVKLLSTELNI
                     QPEEVLVSSTGVIGMFMNMETMKKGVKAIAPEVSEFGGAAASKAIMTTDTVPKEVSFT
                     FEVAGKTATIGGMVKGSGMIHPNLGTMLGYITTDVAIGQTALHAMLLKAADATFNHAT
                     IDGDTSTNDSVFVAATGKLDNQLISSADDAGYAEVEAAVLSICSQLAQLLVKDGEGAT
                     KFVEVLVSGAKTMADAVSIGKSVATSSLVKTAMFGEDANWGRVITAAGYADTEYFDAD
                     VIDIAFSSKNGEIQLCEAGMGVVFDEELAKKILEADEINILIDLHLGTEAASTWTCDF
                     SYDYVKINADYRS"
     gene            1054..1950
                     /gene="argB"
                     /locus_tag="PLT73_04970"
     CDS             1054..1950
                     /gene="argB"
                     /locus_tag="PLT73_04970"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRM19764.1"
                     /translation="MKMNKSNCEKAEMLVEVLPYVNRFRDKIIVIKYGGNAMINEDLK
                     KAVMQDLLLMKSVGMHPILVHGGGPAINDMLKKLKVESHFEQGLRVTDKETMEIVEMV
                     LAGKVNKDIVGTLNQIGGKGIGLSGKDGNLIQARKKYLEKNGEKIDIGYVGEVEAVNK
                     EMLESLIYSGYIPVISSIGMGKDGMSYNINADYVAGAVAKAVQANKFILLTDVEGIFR
                     DYHDKDSLISRITLEEIEALEVDGIISGGMVPKVDCCIDALKGGVAGAHIIDGRLRHS
                     ILLEVFFDEGIGTMIDRDKEGV"
     gene            1954..3150
                     /locus_tag="PLT73_04975"
     CDS             1954..3150
                     /locus_tag="PLT73_04975"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HRM19765.1"
                     /translation="MTNEEIKTKGQTYLMNTFNRGAICMVEGQGSYLKDADGKQYLDF
                     LAGIAVNALGHNHPKVTAAIAEQAAKLVHCSNFYWIEPQVQLAEKLITNSVLDKVFFG
                     NSGAEANEGAIKLARKYGREVHGEGCYEIITADNSFHGRTLATLTATGQKQFHKDYYP
                     FLEGFHYVPFNDFEALAAKVSEKTCAILLEPIQGEGGVYVGDQTYLQQVRALCDEKDI
                     LLIFDEVQTGMGRTGKLFAYEGYGVEPDVMTLAKALGNGVPIGALLAKDAVASHFKPG
                     DHGSTFGGNPLVCAAAFATVQAIEDENILANVNEMGNYFKEKLVAFKEKYDFILDVRG
                     KGLLLGMELAMKGTDIVAKCLEKGVIINCTHDTVLRFLPPLNVTTAEIDKAVAVMDEV
                     FQTIEA"
     gene            3168..>3503
                     /locus_tag="PLT73_04980"
     CDS             3168..>3503
                     /locus_tag="PLT73_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM19766.1"
                     /translation="MPKRTDIKSILVIGSGPIIIGQAAEFDYAGTQACLALREEGYKV
                     ILVNSNPATIMTDVEIADKVYMEPLTLEFIANIIRKERPDALLPTLGGQTGLNMAVEL
                     EEAGILEEYK"
BASE COUNT          946 a          812 c         1006 g          739 t
ORIGIN      
        1 agcaggccct gcagcagcat gaccgcttca aggctatcct gatcaacagc ggcaacgcga
       61 acgcgtgtac aggcaaacaa ggcaaagccg atgtggaaga gaccgtgaag ctcctttcga
      121 cagaactgaa tatccaaccg gaagaggtgt tggtgtcttc gacaggcgtc atcgggatgt
      181 tcatgaacat ggagacaatg aaaaaagggg tcaaggcgat cgcaccggaa gtttccgaat
      241 ttggcggcgc tgcagccagc aaggcaatca tgacgacgga tacggttccg aaagaagtca
      301 gcttcacctt tgaagtcgcc ggcaaaacag ctaccatcgg cggcatggtc aagggatccg
      361 ggatgattca tccgaacctt ggtacgatgc tgggctacat cacaaccgat gtcgcaatcg
      421 gccaaactgc cttacatgcg atgttactga aagccgctga tgcgaccttc aaccacgcga
      481 ccatcgatgg ggacacgagt acgaacgatt ctgtttttgt ggccgctacc ggcaaactcg
      541 ataatcaact gattagttcc gctgatgacg ccggctacgc cgaggttgaa gcggctgtcc
      601 tgtccatctg ctcccagttg gcgcagcttt tggtcaagga cggcgaaggg gcgacaaaat
      661 ttgtcgaagt cttggtttcc ggggcgaaga caatggctga tgcggtttcg atcggcaaat
      721 cggtcgcgac gtcgagcttg gtgaagacgg cgatgttcgg cgaggatgcc aactggggcc
      781 gtgtcatcac ggctgccggt tatgcggaca ccgaatactt cgatgccgac gtgatcgaca
      841 tcgccttctc aagcaagaac ggcgaaatcc agctttgcga agcgggaatg ggtgtcgtct
      901 tcgatgaaga gcttgcgaaa aaaatattgg aagcagatga aatcaatatt ttgatcgacc
      961 tgcatctggg cacggaagcc gccagtacat ggacatgcga cttctcttac gactacgtga
     1021 aaataaatgc ggattacagg agctgagagc gggatgaaga tgaacaagag taattgtgaa
     1081 aaagccgaaa tgcttgtcga agtattgcct tatgtcaacc gtttccgcga taagatcatc
     1141 gtcatcaaat acggcggcaa tgccatgatt aatgaagatc tgaaaaaagc cgtcatgcag
     1201 gatttgttat tgatgaaatc ggtggggatg caccctatcc ttgtccatgg cggcggaccg
     1261 gccatcaacg atatgctgaa aaaactgaag gtcgagagcc acttcgagca ggggctgcgc
     1321 gtgacggaca aagagacgat ggaaatcgtc gaaatggtgt tggcgggcaa agtcaacaag
     1381 gatatcgtcg gcacgctgaa ccagattggc ggcaaaggca tcggtttgtc aggcaaagac
     1441 ggcaatctga tccaagcgcg caaaaaatat ttggaaaaga acggcgaaaa gatcgacatc
     1501 ggctatgtcg gcgaagtcga agcagtcaac aaggagatgc tggagtcact tatctacagc
     1561 ggctacatcc cggtcatttc ctcgatcgga atgggcaagg acggaatgag ctacaacatc
     1621 aatgccgatt atgtagccgg cgctgtggcg aaagccgtcc aggcaaacaa attcatcctg
     1681 ctgaccgatg tggaagggat tttccgcgac tatcatgata aggattctct tatttcccgg
     1741 atcacgctcg aggagatcga agcattggaa gtggacggca tcatctcagg cggcatggta
     1801 ccgaaagtgg actgttgcat cgatgcgttg aagggcggcg ttgccggagc gcacatcatc
     1861 gatggtcgtc tgcgccactc gattttattg gaagtttttt tcgacgaagg gatcggcacg
     1921 atgatcgatc gcgataagga aggggtataa agaatgacga atgaagaaat aaaaacaaaa
     1981 gggcaaacct acttgatgaa taccttcaac cggggagcga tctgtatggt tgaaggacaa
     2041 ggcagctatc tgaaggatgc ggacggcaag caatatttgg acttcctggc aggcattgcc
     2101 gtgaatgcgt tagggcataa tcacccgaaa gtgacggctg cgatagcgga acaggcagcc
     2161 aaactggtcc actgctcgaa cttttattgg atcgagccgc aagtgcagtt ggcggaaaaa
     2221 ctgatcacga attccgttct ggataaggtc tttttcggca atagcggtgc ggaagccaat
     2281 gaaggagcca tcaaattggc gcgtaaatac ggacgtgagg tccacggcga aggctgctac
     2341 gagatcatca ctgccgataa ctcgttccac ggccgcacgt tggcgacgtt gacggcgacc
     2401 ggccaaaaac aattccataa agattactat ccgttcctgg aaggcttcca ctacgttccc
     2461 ttcaatgact ttgaggcttt ggccgccaaa gtttcggaaa agacatgcgc aatcctgctg
     2521 gaaccgatcc aaggcgaagg cggcgtctac gtgggcgatc aaacttacct gcagcaagtg
     2581 cgcgcacttt gcgacgagaa ggatatcctg ttgatctttg atgaagtcca aacgggcatg
     2641 ggccgaacag gcaagctgtt cgcctacgaa ggctacggtg tggaaccgga tgtgatgacg
     2701 ttggcgaaag ctttgggcaa tggcgttccg atcggcgcac tgttggcgaa ggatgccgtc
     2761 gcttcccatt tcaaaccggg tgaccacggt tcgactttcg gcggcaatcc gcttgtttgt
     2821 gcggcggctt ttgcgactgt tcaagcgatc gaggacgaga atatcctggc gaatgtaaat
     2881 gagatgggca actatttcaa agagaaatta gtggcattca aggaaaaata tgactttatc
     2941 ctcgatgtac gcggcaaagg cttactgctc gggatggaac tggcgatgaa gggtaccgat
     3001 atcgtcgcga agtgcttgga aaaaggcgtc atcatcaatt gcacgcatga tacggtcctg
     3061 cgtttcctgc cgccgctgaa tgtgacaacc gcggaaatcg acaaggctgt tgcagtgatg
     3121 gatgaggttt ttcaaacaat tgaagcttaa agaaaggatg gcaaagcatg cctaaacgaa
     3181 cagacatcaa gtcgatcctc gtgatcggct cgggacctat catcatcggc caagccgcgg
     3241 aatttgacta tgctggaacg caagcatgtt tagccttaag ggaagaagga tacaaagtca
     3301 tcctggtcaa ctccaacccg gctacgatca tgacggacgt tgagattgcc gataaagtct
     3361 atatggaacc actgacattg gagttcatcg cgaacatcat ccgcaaagag cgcccggatg
     3421 ccctattgcc gacattgggc ggccagaccg gtctgaacat ggcggtcgag ctggaggaag
     3481 ccggcattct ggaggagtac aag
//
LOCUS       DAOMUJ010000157         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7596_length_3502_cov_2.798085, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000157 DAOMUJ010000000
VERSION     DAOMUJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7596_length_3502_cov_2.798085"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="glmS"
                     /locus_tag="PLT73_04985"
     CDS             <1..1230
                     /gene="glmS"
                     /locus_tag="PLT73_04985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRM19767.1"
                     /translation="DAIAMIQLTNQYVEIHDGQLVIVTADKVTIESFDGTIRERAPYT
                     AHLDSSDLEKGTYPFYMLKEIDEQPGVMRKIVKEYSDDKGGFNIDAALLKELAEADRI
                     HIIACGTSYNAGWVGKSLFENIAKIPTEVHLSSEFSYNTPLIQGKPFFIYLSQSGETA
                     DSRQVLVKTNEWNFPAVTITNVPGSTLSREAKYTLLLHAGPEIAVASTKAYTGQIAVQ
                     AMLCEILAKTKGVQTDIDMAHELSIVANAMDVIINDKETIHDFAIDYVQSARNAFYIG
                     RGLDYYVCMESSLKLKEISYIQAEGFAAGELKHGTIALIEEGTPVLAVITQKNVASHT
                     RGNVEEVRSRGANTLIFAMEGLEKKGDQFVMPAVHPALTPLLAVVPGQLFAYYATLER
                     GYDVDKPRNLAKSVTVE"
     gene            complement(1429..1602)
                     /locus_tag="PLT73_04990"
     CDS             complement(1429..1602)
                     /locus_tag="PLT73_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19768.1"
                     /translation="MTLKGTSSLQRTTYFHLYFHDSTLIFQKQLIWIRDDSLTFIILE
                     DPAPPQRGLFEGG"
     gene            1875..3269
                     /locus_tag="PLT73_04995"
     CDS             1875..3269
                     /locus_tag="PLT73_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HRM19769.1"
                     /translation="MDIISIIIILAVFVALLFALYRLGSKHVKFSRRVFIALALGIVF
                     GAAIQLLFAPDSTITLTAIDWINIVGNGYVRFLQMLIMPLIFVSIVGAFTRIEETKNL
                     GKISGSVLGTLLGTTAIAALIGWASVILFNLDGAQFTQGTAETARIEALTTQQTQVAD
                     LTIPGQVLSFIPTNIFQDLAGLRSTSTIAVVVFSAFVGIAYMGIKKKQPDNAALFKKG
                     LQVIQSIVMRIVTLVLRLTPYGILALMTKMMATSSYQAIVNLGLFVVASYAALLVVLL
                     VHSLILAGVGVNPITYFKKAFPVLSFAFTARSSAGALPLNVETQTKALGVDDASANFA
                     ASFGLSIGQNGCAGVYPAMLATIVAPTLGIDVTSIGFVLTLILVVTISSFGVAGVGGG
                     ATFAALIVLGAMNLPVTIAGLVISVEPVIDMMRTLTNVNDSMLAGVVSSRNIGEFDSS
                     ILNDEAAVVDGDAI"
BASE COUNT          911 a          820 c          809 g          962 t
ORIGIN      
        1 gatgctatcg ctatgatcca actgacaaat cagtacgttg aaatccatga tggtcagttg
       61 gttattgtaa cagctgacaa ggttacgatt gaatcattcg atggcacaat tagggaacgt
      121 gccccataca cagctcatct cgattcaagt gatttagaaa aagggaccta cccattctac
      181 atgttgaaag aaatcgatga acaacccggc gttatgcgca aaatcgttaa agaatattcc
      241 gatgacaaag gcggattcaa cattgatgcc gctttactta aagaattggc agaagcggat
      301 cgcattcata ttatagcttg cgggacaagc tataatgcag gttgggtcgg caaatccttg
      361 ttcgaaaata ttgctaaaat ccctacagaa gtgcatttgt ccagtgagtt ttcatataat
      421 acgccattaa tccaaggtaa accattcttt atttacctct ctcaaagtgg cgaaacagca
      481 gacagccgcc aagtgcttgt aaaaacaaat gaatggaact tccctgctgt gacgattaca
      541 aacgtaccag gttctaccct ttcacgtgaa gcaaaatata ctttattgtt acatgccggc
      601 cctgaaatcg ctgttgcttc aacaaaagct tacactggcc aaatcgcggt ccaagcaatg
      661 ctttgcgaaa ttttagcaaa aacaaaaggt gtacaaaccg atatcgacat ggctcacgaa
      721 ttaagcattg ttgctaatgc aatggatgtc atcatcaacg ataaagagac cattcacgac
      781 tttgcaatag actatgttca gagcgcccgt aatgctttct atatcggtcg cggattggat
      841 tactatgtat gtatggaatc atcgctgaaa ttaaaagaaa tttcttacat acaagcggaa
      901 ggatttgcgg ctggagaatt gaaacacggc acaatcgctt tgatcgaaga gggtacacca
      961 gtccttgcgg tcattaccca gaaaaacgtt gcttcccaca ccaggggtaa cgttgaggaa
     1021 gtacgttccc gtggagccaa tacgcttatc tttgctatgg aaggattgga aaagaaaggc
     1081 gatcaattcg taatgcctgc cgttcatcca gcgttaacac ctttattagc tgttgttccg
     1141 ggacaattat tcgcctacta cgcaacactt gaacgtggct atgacgttga caaaccacgc
     1201 aaccttgcta agagtgttac tgtagaataa taaaacgcct tgaaaaaact caatttattc
     1261 ctattctcgt acagatggag taaattttgg ttctctgtca aatagcgttg ctgagatgct
     1321 tacctattaa aatatgatga tcacattcac aacccccaaa aagacttttt cttttggggg
     1381 tttgtgtttt tttatcgtct gaagaaaatt gaattggctg aataacgttc agcctccttc
     1441 aaacagaccg cgttgaggcg gtgctggatc ttccaaaatt ataaacgtta aagaatcatc
     1501 tctaatccaa ataagttgct tttgaaaaat aagcgtgcta tcatgaaaat acaaatgaaa
     1561 ataagttgtg cgttgaagag aagaagtacc tttcagggtc atcaaagaga gcctcagtcg
     1621 gtgagaagag gcatggctag taaagcgaac atgcgtctcg gagcaagatg atgcgttagt
     1681 gtcatttcgg tttgtacccg atagcgtaca gaagtatggc tgattttgcg tacttcataa
     1741 ggcaccatcc gcgagggtcg tgcgaacaag ggtggatcac gaagagcttt cgtccctgta
     1801 ttccaagcat ttggagtata gggacgaggg cttttttacg ttctcgggat agttaaatta
     1861 aaggaggaat acaaatggat atcatttcca tcatcattat tttggccgta tttgtggcac
     1921 tgcttttcgc actttatcgt ttaggctcca aacatgttaa gttctctaga cgcgttttca
     1981 tcgcgctggc tttagggatc gtcttcgggg ccgccatcca gttacttttt gctcctgaca
     2041 gcacaatcac actaaccgcc atcgattgga tcaatatcgt cggtaacggt tatgtccgct
     2101 tcctgcaaat gctgatcatg ccattgattt tcgtttcgat cgtgggcgct tttacccgca
     2161 tcgaagaaac aaagaacctg ggcaaaatca gcggatccgt tcttggaact ttgctgggca
     2221 caaccgccat cgcggctctg attgggtggg catcggtcat tctcttcaat cttgatggcg
     2281 cacaattcac gcaaggtacc gctgaaactg caagaatcga agctttgact acccaacaga
     2341 cacaagtcgc tgacttgacg attcctggtc aagtgctcag cttcatcccg accaacatct
     2401 tccaggatct ggccggtctg cgcagcacga gtacgattgc cgtcgtggtc ttttccgctt
     2461 ttgtcggaat cgcttatatg ggcatcaaga aaaaacaacc cgacaatgcc gctctattca
     2521 agaaagggtt gcaagtcatc caatcgatcg tcatgcgcat cgttactttg gtccttcgtc
     2581 tgactccata cggaatcttg gccttgatga ccaaaatgat ggcgacatcc agttaccaag
     2641 ccatcgtcaa cttaggactg ttcgtggtcg cttcctatgc cgcgttactg gtcgtcctgc
     2701 tggtgcacag cttgattttg gctggagtcg gcgtcaatcc gattacctac ttcaaaaaag
     2761 ctttcccggt attgagcttc gccttcactg cccgttccag cgccggcgca ctgccgttga
     2821 acgttgaaac acaaacgaaa gctttgggcg tcgatgatgc ttccgccaac ttcgccgcca
     2881 gcttcggctt gtcgatcggt cagaacggtt gcgcgggcgt ctatcctgcc atgttggcga
     2941 ccatcgtggc tccaactttg ggcatcgacg tcaccagcat cggttttgta ttgacattga
     3001 tcttggtcgt tacgatcagt tccttcgggg ttgccggtgt cggcggcggt gctactttcg
     3061 ccgctttgat cgttctgggc gccatgaact tgcctgtcac aatcgccgga cttgtcatct
     3121 ccgttgaacc ggtcatcgac atgatgcgca cgctgacaaa cgtcaacgac agcatgctgg
     3181 ctggcgtcgt ttcttcccgc aacatcgggg aatttgattc ttccatcctg aatgatgaag
     3241 ctgctgttgt cgatggggat gcgatttagt cttctattta tatctgtcct gacgaaggac
     3301 tcggctgcct gcaatttgcg ggcagctttt tttgtattcg gataccattg agcggtgaag
     3361 tcgctgcgct cctccggtga ggcttcccac accggagaac agcggtaatg tgcgggctct
     3421 tctcctgcga gcagattctc atcggagcac aagacttaaa tgtaagctat actccggcgt
     3481 agcctccgtt cgccggagga aa
//
LOCUS       DAOMUJ010000158         3489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7635_length_3489_cov_2.650553, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000158 DAOMUJ010000000
VERSION     DAOMUJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_3489_cov_2.650553"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1240
                     /locus_tag="PLT73_05000"
     CDS             47..1240
                     /locus_tag="PLT73_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM19770.1"
                     /translation="MEKGIRNFEFQNPTKIVFGKDQIKRIGDLIPQDAKVLILYGGGS
                     VKKFGTFDRVVEALGNREWAEFGGIEANPTYETLIQAVEKIKAEAYDYLLAVGGGSVI
                     DGVKFVAAGAVFDGNPIDMFGSGVGKGLPVTKALPFGTVLTLPATASEMNNNSVVTFV
                     EKQAKISFASPHTYPQFSILEPEATYTLPKRQLANGVIDSFIHVMEAYLTYPIDARVQ
                     DRWAEGLLQTLIEIGPDVVDEDNHDYAIRANFMWTATNALNRFISPGVPQDWSTHALG
                     HEMTLAFGIDHARTLAIVLPAMMKVRKQQKWDKLLQYAERVWDIRSDSDLISDEEKID
                     LAIKKTEDFFVSLGAPIRFSDVELGESDIPGLVEALVRHEKENISERGDFTSEDARAV
                     YTAAL"
     gene            1406..3406
                     /locus_tag="PLT73_05005"
     CDS             1406..3406
                     /locus_tag="PLT73_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HRM19771.1"
                     /translation="MENNQSIFETICRLREEEPGLPYRFQDERTAGQKDVLYVLASEG
                     IPFWRKEDLAKECCGILKDLVNKEDTILTDPVLRHFLEHYPICSYFIELRERVRITLE
                     AETSSRERLFHLGMRLARSGTDPEQVKLGIILLGFFPYDTTKQIMRVLGYHSEYTLYV
                     LESIQYVFPMQNNFIFELAKHTVGYGKLAAMFLLKPVRWEQQHWMMHEGIKSDFLANI
                     YTNLCIQKTDMRAYFKKTEITAANFTDFAYLICYADYNNDSLTIDAQLDFLYKFIEKR
                     DFAKNFIDLGALVSIWYQVVDFWQQDYDFISQNETKYRRTKTMWDTRIARYEKLVHKV
                     ESFLHQPKWRHIVYQEISAPNESDNLIMKVLVYLNMHPDFPAFMEVLSRQPLGFNMLD
                     FFLKINPEFYFDDVCEYLEAILNPDLYALPLEIEEPENPSVTDLMRADEWLLRLFEVM
                     SEKRKYNEVWCIRGIHYRHAGVRKKAVQVLLQNRKKWSDQVERELQIALEKEPNSNLK
                     RQINRLLQPENQKDKKESRYLKSKQPPLSYAHTDKELVHTYIAGTQFHELSGVADFLK
                     PGDLLQLVRETDNAYDANAIAVATQAGYMLGYVPRSENAVLANLIDAEERLYAILESP
                     TVEMERPKITIVLKRTFFQAQPNGEGQGIILPFPPPKKKKYE"
BASE COUNT          969 a          810 c          891 g          819 t
ORIGIN      
        1 gtgctacaat gaaaaaagaa taaaaaacga aaagtgagtg atgagattgg aaaaaggaat
       61 acgtaatttt gagtttcaaa atccgacaaa gattgttttc ggcaaggacc agatcaaacg
      121 catcggggat ttgatcccgc aggatgcgaa agtattgatt ttgtacggcg gcggcagcgt
      181 caaaaaattc ggcaccttcg atcgcgtcgt cgaagctttg ggcaaccgcg agtgggctga
      241 attcggcggc atcgaggcga atccgaccta cgaaacgctg atacaggcag tcgagaagat
      301 caaagcggaa gcctatgatt acttgttggc agtcggcggc ggcagcgtca tcgacggcgt
      361 gaaattcgtt gcggcaggtg cggtgttcga tggcaatcct attgatatgt tcggcagcgg
      421 cgtaggcaag ggattgccag tcacaaaagc attgcctttc gggactgtat tgacgttgcc
      481 ggctacggca tcggaaatga acaacaattc tgttgtcact tttgtggaaa agcaagcgaa
      541 aatcagtttc gccagcccac atacttatcc acaattctcg atcctggaac cggaagcgac
      601 ctacacgtta cccaaacgcc agctggcgaa cggtgtcatc gattccttca tccatgtgat
      661 ggaagcctat ctgacttatc caatcgatgc aagggttcag gaccgttggg cggaagggtt
      721 actgcagacg ttgatcgaaa tcggcccgga cgtagtcgat gaggacaacc acgactatgc
      781 gatccgcgcc aatttcatgt ggacggccac gaatgcgctc aatcgcttca tttccccagg
      841 tgttccgcag gattggtcta cgcacgcttt gggacatgag atgacgttgg cgttcgggat
      901 tgaccatgcg cgcacgctag cgattgtgct tccggcgatg atgaaggtac gcaagcagca
      961 gaagtgggat aaattgcttc agtacgcgga acgggtgtgg gatatccgca gcgattccga
     1021 tctgataagc gatgaagaaa aaatcgatct ggcgatcaaa aagaccgaag acttttttgt
     1081 aagccttggc gcgccgatcc gtttctctga tgttgagctg ggtgagagcg acattcccgg
     1141 tcttgtggaa gcactcgtcc gccacgaaaa agagaatatt tccgagcgtg gggacttcac
     1201 aagtgaagat gcaagagccg tatatacagc tgcgctttaa aattatgttt aacctgtatc
     1261 gaactccctt taatcgggga gtttttttgt gcattttgct cctgttaatt taacaatttt
     1321 aaaaactaac atagtgacgc tctgtaatat atggtaagat taaagaaata gagtatcgga
     1381 gtgaacagga aagaagggtt gccatttgga aaataaccag agcattttcg agacaatctg
     1441 ccggctgcgt gaagaagagc ccggtctgcc ttatcgattc caggatgagc ggacggctgg
     1501 gcaaaaagat gtactgtacg tccttgcatc cgaagggatt cctttttggc gcaaagagga
     1561 tttggcgaag gaatgctgcg gcatcctgaa agaccttgtc aataaagaag acaccatatt
     1621 gacagatcct gtcctgcgtc attttctgga gcattatcca atctgcagtt acttcattga
     1681 attgcgcgaa agagtacgga ttactttgga agcggaaaca agttcgcggg agcggttgtt
     1741 ccacctcggc atgcgcctgg cccgtagcgg caccgatccg gaacaggtga agttgggcat
     1801 cattcttttg ggcttctttc catatgatac gaccaagcag attatgcgcg tcttgggtta
     1861 tcacagtgag tacacccttt acgtgctcga gagcatccaa tacgtttttc ccatgcagaa
     1921 caatttcatt ttcgaattgg ctaagcacac agtcggttac gggaagctag cagccatgtt
     1981 cttgctgaag ccagtcagat gggagcagca gcattggatg atgcacgaag gtatcaagag
     2041 cgatttcctc gccaacatct ataccaatct ctgcatccag aaaaccgata tgcgcgccta
     2101 tttcaaaaag acggaaatca cggccgccaa tttcacggat ttcgcctacc tcatctgtta
     2161 tgccgactac aacaatgata gtttgaccat cgatgcccaa cttgatttcc tgtataagtt
     2221 catcgaaaag cgtgattttg cgaaaaactt catcgatctt ggcgcgctcg tcagcatctg
     2281 gtatcaagtc gtcgactttt ggcaacagga ttacgacttc atcagtcaaa acgagacgaa
     2341 gtaccgccgc accaaaacca tgtgggatac ccgcatcgcc cgttacgaaa agttggtaca
     2401 taaagtggag agtttccttc atcaaccaaa atggcgccat atcgtctacc aggaaatttc
     2461 cgctccgaac gaatcggata acctgatcat gaaggtactc gtctatctga atatgcatcc
     2521 cgattttccg gctttcatgg aagtgttgag ccggcaaccg ttgggattca acatgcttga
     2581 tttctttctg aaaatcaatc ccgaattcta tttcgacgat gtctgcgaat acttagaagc
     2641 gatcctgaat ccggatcttt acgcgcttcc cttagagatc gaagaaccgg aaaatccgag
     2701 cgttacggat ctgatgcgcg ccgacgaatg gttgctgcgc ctattcgaag tgatgagcga
     2761 gaaacgcaaa tacaatgaag tctggtgtat ccgcggtatc cattaccgcc atgccggtgt
     2821 acgcaaaaaa gcggtccaag tattgctgca gaaccgaaaa aaatggtctg accaggtgga
     2881 acgcgaacta cagattgcac ttgagaagga gccgaacagc aatctgaaga ggcaaatcaa
     2941 cagactgctg caacctgaaa atcagaaaga caaaaaggaa agccgatatc tgaagtcgaa
     3001 gcagccgcca ttgagctatg cccatacgga taaggaattg gtgcatacct acatcgccgg
     3061 cacgcagttt catgaactga gcggcgtcgc tgatttcctg aaaccgggag acctgctgca
     3121 actcgtccgc gagaccgata atgcttatga tgcgaatgcc atcgccgttg cgacgcaagc
     3181 cggctacatg ttgggttacg tgccacgatc agagaatgct gttctggcga atctgatcga
     3241 tgccgaggag cgtctttacg caatccttga atcaccgaca gttgagatgg aacggccgaa
     3301 aatcacgatt gtcctgaaac gtactttttt ccaagcgcaa ccgaacgggg aaggccaagg
     3361 catcatcctg ccgttcccac cacccaagaa aaagaaatac gaataagaag aagaaaagcg
     3421 gaacgggttc gtatagccct gagtaaggaa acactgtgac ctaatcggtc aagtactctg
     3481 atgtcacag
//
LOCUS       DAOMUJ010000159         3465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7708_length_3465_cov_2.128739, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000159 DAOMUJ010000000
VERSION     DAOMUJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_3465_cov_2.128739"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /gene="dnaK"
                     /locus_tag="PLT73_05010"
     CDS             complement(<1..1360)
                     /gene="dnaK"
                     /locus_tag="PLT73_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM19772.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGGEAKIIPNPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHMGDAGYKVEVEGKSYTPQEVSAMILQYLKGYA
                     EDYLGEKVDKAVITVPAYFNDAQRQATKDAGRIAGLEVERIVNEPTAAALAYGLDKTD
                     KDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDNKIIDYLVAEFKKE
                     NGIDLSNDKMAKQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLELTLTRAKF
                     DEITYDLVERTKIPVRQALKDAGLSTSEIDEIILVGGSTRIPAVIEAVRKEAGKEPNK
                     SVNPDEVVAMGAAIQGGVISGDVKDIVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDVHVLQGERPMAADNKTLGRFQLTDIPAAPRGVPQIEVSFDID
                     KNG"
     gene            complement(1406..1942)
                     /gene="grpE"
                     /locus_tag="PLT73_05015"
     CDS             complement(1406..1942)
                     /gene="grpE"
                     /locus_tag="PLT73_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM19773.1"
                     /translation="MDNNEELVEQQETTVETSMEEGQETVTEISEVEALQATLSETED
                     RLLRLQAELANIQKRNAKERQDAAKYRSQSLAQELLPVMDSLEHALAIEVEDEKAVNL
                     KKGLEMVLNLFTDAFAKEGITAINPIDQPFDPNFHQSIQVQPAAEGQAPDTVVAVVQK
                     GYTLKERVLRPAMVVVSQ"
     gene            complement(1965..>2388)
                     /locus_tag="PLT73_05020"
     CDS             complement(1965..>2388)
                     /locus_tag="PLT73_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HRM19774.1"
                     /translation="LIIRKYVDAKIDFTAIFNDIVAKLEHDRFHVGGSMNLLNHLDST
                     MDKNKVKDILSMLNGSPDIHALFSNPYEGVGVKIGTELNNELLHNFSLITATYDTAGN
                     GKGLIALLGPTNMPYQKMISIMKFMRYELSDSLNDLNK"
     assembly_gap    2389..2398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2399..3005)
                     /gene="hrcA"
                     /locus_tag="PLT73_05025"
     CDS             complement(<2399..3005)
                     /gene="hrcA"
                     /locus_tag="PLT73_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRM19775.1"
                     /translation="MKILLTERQLLIFDLIVRHYIEFEEPLGSKTLLKESSLPLSSAT
                     IRNEMMRIEELGFLEKMHSSSGRIPSIQGYRYYVDQLLGKESEEVADEVKQMIKHGFQ
                     NPNREVQQVVEKSAEMLSFLTNYTALAIGPETKDSRLTGFRLVALNEGHVMAILVTDK
                     GHVENQIFSVSPGFSATEIEKIVNIFNQELVGRTLQEVFVKL"
     gene            complement(3133..>3465)
                     /locus_tag="PLT73_05030"
     CDS             complement(3133..>3465)
                     /locus_tag="PLT73_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRM19776.1"
                     /translation="GYIDGIRYHNHGPIQQYLAPLRENNLPIIRQQQLSKNEQMEEEM
                     ILGLRTMAGVSQQHFAEKFQTSLLDQYASVISDLVTEGLLMLDGDRIRLTQRGVFLGN
                     EVFRSFLM"
BASE COUNT          838 a          833 c          726 g         1058 t
ORIGIN      
        1 taccattttt atcgatatcg aatgatactt cgatttgtgg aacgccacga ggtgcagctg
       61 gaatatcagt caattggaaa cgacccaaag ttttgttgtc ggctgccatc ggacgctccc
      121 cttgcaacac atgtacatca acagcaggtt gattgtcggc tgcagtcgag aagacttgtg
      181 acttgcttgt agggattgtt gtattgcggt cgatcaattt ggtgaacacg ccacccattg
      241 tttcgatacc caatgataaa ggtgtaacgt ccaatagtac gatgtctttt acgtcgccag
      301 agatgactcc gccttggatg gcagcaccca ttgcgacaac ttcatccggg ttaacggatt
      361 tgttcggctc tttgccggct tctttgcgga ccgcttcgat gactgcagga atacgtgttg
      421 aaccaccaac caaaatgatt tcgtcgattt cggaagtgga taaaccggca tccttcaacg
      481 cttggcgtac aggaattttt gtgcgttcaa ccaagtcata cgtgatttca tcaaatttcg
      541 cgcgggtaag cgtcaattcc aagtgcaatg gaccagcttc tccggcagtg atgaacggca
      601 ggctgatttg agttgacgtt acgcccgaca aatctttctt ggctttttcg gcagcatctt
      661 tcagacgttg tttcgccatt ttgtcgtttg aaaggtcgat tccgttttct ttcttgaatt
      721 ctgcaaccaa gtagtcgatg atcttgttat cgaagtcatc gccacccaat ttattgtcgc
      781 cggctgtact caatacatcg aagacgccgt cgcccaattc aaggatggaa acgtcgaatg
      841 taccgccgcc caagtcgaat acaagaattt tttcgtcttt atcggtttta tccaatccgt
      901 atgccaaagc agcagccgtc ggttcgttga cgatacgctc tacttccaga ccggcaattc
      961 tgcccgcatc tttagtcgct tgacgctgtg catcgttgaa gtaagctgga acagtaatta
     1021 cggctttgtc aactttttca cccaggtagt cttccgcata acctttcaga tattgaagga
     1081 tcatcgctga aacttcttgt ggtgtgtagc tcttgccttc cacttccact ttgtagccag
     1141 catcgcccat atgacgtttg atggagctga ttgtattcgg gtttgtcact gcttgacgct
     1201 ttgcaacctc gcccacttgg atttcgccat tcttgaatga tacgacagat ggtgtcgtgc
     1261 gatttccctc tggatttgga atgattttag cttctccgcc ttctaatact gctacagctg
     1321 agtttgttgt tcctaagtca ataccgataa ttttgctcat gatgttattc ccctctatat
     1381 gaaattttta tgtttatcaa taaaattatt gtgatacgac aaccattgct ggccttaata
     1441 cacgttcttt caaggtgtat cctttttgca ctaccgcgac gactgtatca ggagcttgcc
     1501 cttcagcagc cggttgcact tggatggatt gatggaaatt cggatcgaaa ggctgatcaa
     1561 tcggattgat cgcagtaatc ccctctttgg cgaaagcatc tgtaaacaag ttcaagacca
     1621 tttccaaacc tttcttcagg ttcacagctt tttcatcttc aacttcaatc gctaatgcgt
     1681 gctccaggct atccattacc ggcaataatt cctgcgctaa tgactgggaa cggtatttcg
     1741 ccgcatcttg tctttctttc gcgttgcgtt tctggatatt tgcgagttct gcttgcaggc
     1801 gcaataagcg gtcttccgtt tcggataagg ttgcctgtaa tgcttcaacc tcacttattt
     1861 ctgtgactgt ttcttggccc tcttccattg atgtttcaac ggttgtttcc tgttgttcca
     1921 ctaattcttc gttattgtcc acttcaaaat ctcctttgtc gtgcttattt gttcagatca
     1981 ttcaagctat cagaaagttc atagcgcata aatttcatga tgctgatcat tttttgataa
     2041 ggcatattgg ttggccccag tagtgcgatc aggcctttac cgttgccagc cgtgtcataa
     2101 gtagccgtga tcagactaaa attatgcagc agctcattat tcaattccgt accgatcttg
     2161 accccgacgc cctcgtaggg gttagaaaaa agcgcatgga tgtccggaga cccattcaac
     2221 atgcttaaaa tgtctttgac tttattcttg tccatcgtcg aatccaaatg gttcagaaga
     2281 ttcatgctac cacctacatg gaagcgatca tgctccaact ttgccacaat gtcattgaag
     2341 atcgctgtga aatcgatttt tgcatcgacg tacttccgga taatcaacnn nnnnnnnngc
     2401 agtttgacga atacttcctg cagcgtgcgg ccgaccagtt cctgattgaa aatgttgacg
     2461 attttttcta tctcagtggc tgagaagccc ggcgaaacag aaaaaatctg attttccaca
     2521 tgtcctttgt cagtcaccaa tatcgccatc acatgtcctt cattgagtgc gaccagccgg
     2581 aagcccgtca aacggctgtc ctttgtttca ggtccgatcg ccaaagcggt ataattcgtc
     2641 aaaaaggaca acatttcagc tgatttttcg acgacttgtt gcacttcacg attaggattc
     2701 tgaaagccgt gcttgatcat ttgcttgacc tcgtcagcga cttcttcgct ttctttgcct
     2761 aaaagttgat cgacatagta acgatagcct tggatggatg gtatcctgcc ggacgaagaa
     2821 tgcatctttt ccaggaagcc caattcttcg atccgcatca tttcgttgcg gattgttgct
     2881 gagcttaaag gcaaagaaga ctccttaaga agtgtcttcg acccgagcgg ctcctcgaat
     2941 tcgatgtaat gcctgacaat caaatcaaaa attagcaact gtctttctgt taacaagatt
     3001 ttcacctcac tttagcactt gaccacaatg agtgctaacc acaaaataaa tttatcacgg
     3061 gtaacagact ttgtcaacgg aatacaatca tttttcataa cattatcagc agccgatcgg
     3121 atacgagcgt attcacatca agaaagaacg aaatacttca ttgccgagaa aaacgcccct
     3181 ttgtgtcaga cggattctgt cgccatcaag catcaacaag ccttctgtaa ccaaatccga
     3241 aatgacgcta gcatactgat ccagcaacga agtctggaat ttttcggcaa aatgctgttg
     3301 gctgacacct gccatcgtgc gcagccccag aatcatctct tcttccattt gttcgttttt
     3361 cgataactgt tgctgccgga taatcggcag attattttcc cgtaatggcg ccagatattg
     3421 ttggatcggt ccatgattgt ggtaacggat gccatcgata tagcc
//
LOCUS       DAOMUJ010000160         3463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7717_length_3463_cov_2.111209, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000160 DAOMUJ010000000
VERSION     DAOMUJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_3463_cov_2.111209"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>267)
                     /locus_tag="PLT73_05035"
     CDS             complement(<1..>267)
                     /locus_tag="PLT73_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM19777.1"
                     /translation="RKDWLHKKAYTLANRYDMVVVEDLDLRALGQTLKLAKNQQDNGF
                     GNFRLYLKYKLEQQGKYFVKAPKSFASTQICSDCGVKNTALKGD"
     assembly_gap    268..277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<278..1170)
                     /locus_tag="PLT73_05040"
     CDS             complement(<278..1170)
                     /locus_tag="PLT73_05040"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM19778.1"
                     /translation="MSVETVNRGYVYSGTPISDEAERYFRQCFGADRLMYNLHVAHLY
                     AYLEKNGHTIGDKLPTFQEMGMPTVKAFKQTRMDDDGNKYLYEVDAFASNEAKQHFKK
                     AMNSFNKNAFKGQFKKSALKRQKTLGIEPTFRDLKGMPKFHSRKNSKLSYTTFNQGDN
                     IFIDENNVLHLPLAQKSKLSGAAIKLNTHRPMPKDGRIQHVTITTDSQGRFAVTINVA
                     FEMEIESIGIPAAERILGLDYSQSDFFVDSEGKKANYPHYYRVMEDRLAKEQRKLSRM
                     VQGSKRWERQRLVVAKLHSKVA"
     gene            complement(1416..2258)
                     /locus_tag="PLT73_05045"
     CDS             complement(1416..2258)
                     /locus_tag="PLT73_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HRM19779.1"
                     /translation="MTYTLGIYEKSMPGNLSWKEKLTIAKNAGYDFLEMSVDETDEKL
                     ARIEWSKQERKELVDAMYEVGLPIRSMCLSGHRKYPLGSHDPLIRAKSLEIMEKAIEL
                     ADDLGIRIIQLAGYDVYYEDGDQQTKEYFIGNLQKAVDMASKNGILLGFETMETPFMN
                     TVEKAMEYVDIIQSPYLQVYPDTGNLKNASLSCTNSVFEDIQTGRGHMVAAHLKETIP
                     GHFREIPFGTGHVSFEETIKTMWKEGVRRFVAEFWYTGQENWLEDIQFAHTFLRGKFQ
                     KVGH"
     gene            complement(2233..2961)
                     /locus_tag="PLT73_05050"
     CDS             complement(2233..2961)
                     /locus_tag="PLT73_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM19780.1"
                     /translation="MTYTELKKRVLEANLSLPEYGLVKFTWGNVSEIDREKQIIAIKP
                     SGVEYADMTVEDIVVVDMDGNVVEGSLKPSSDLDTHLELYRNFKNIGGVVHTHSPWAT
                     TLAQGGVEIPAYGTTQGDYFYGTIPCTRRMTSEEIAGSYELETGKVIVETFEAIDEDQ
                     VPGVLVHSHGPFAWGKDAFEAVHNMVVMEEVAMMSWRNRVMNPGIESMQQELLDKHFL
                     RKHGPEAYYGQVKEEPHDLHVRNL"
     gene            complement(2973..3260)
                     /locus_tag="PLT73_05055"
     CDS             complement(2973..3260)
                     /locus_tag="PLT73_05055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM19781.1"
                     /translation="MKVLAACGSGMGSSQIIKMKITNVFKKLNIPLEIHHCAVSEAKT
                     LAPNYDVVVCSNSLLDVFKNVDQSKTKIIGLKNLLSEKEIEEKVREIVLGE"
BASE COUNT          782 a          855 c          744 g         1072 t
ORIGIN      
        1 tatcgccctt gagggccgta tttttgacgc cacaatccga acagatttgt gtggaagcaa
       61 agcttttggg cgctttcacg aaatatttac cttgttgctc aagtttgtac ttgagataca
      121 ggcgaaagtt gccgaaccca ttatcttgtt ggtttttcgc caacttcaag gtttgtccaa
      181 gcgcacgaag gtcgaggtct tccacgacaa ccatgtcata gcgattcgcg agggtgtagg
      241 ctttcttgtg gagccagtct ttccgctnnn nnnnnnngca actttcgaat ggagtttcgc
      301 taccaccaac cgttggcgtt cccatcgttt agagccttgc accatgcggg atagcttgcg
      361 ctgttctttt gccagacgat cttccatgac tcggtagtag tgcggataat tggctttctt
      421 accttcacta tctacgaaga aatcggattg ggagtaatcc agcccaagaa tgcgttcagc
      481 agcagggata ccaatgcttt cgatttccat ttcaaacgcg acattgatgg tcaccgcaaa
      541 ccttccttga gagtccgttg ttatcgtgac atgctgtata cggccgtcct tcggcatggg
      601 gcgatgtgtg ttcaatttga ttgccgcccc tgacaattta ctcttttgag caagcgggag
      661 atggagaacg ttgttttcat caatgaagat attgtcacct tgattgaatg tggtatagct
      721 cagcttactg ttcttgcgag aatggaattt cggcatccct ttaaggtcgc ggaaggtagg
      781 ctcaatcccg agcgttttct gccgtttcag tgcagacttc ttgaattgcc ctttgaacgc
      841 attcttgttg aatgaattca tcgctttctt gaaatgctgt tttgcttcgt tcgaagcgaa
      901 tgcatccact tcatacaggt atttattgcc atcgtcatcc atgcgagttt gcttgaacgc
      961 tttgacggtt ggcatcccca tctcttggaa tgtaggcagc ttgtcaccga tggtgtgccc
     1021 attcttttca agatacgcat acaaatgggc cacgtgaagg ttgtacatca accggtccgc
     1081 gccaaaacat tgacggaaat agcgttctgc ttcatcagat atgggtgttc cgctatacac
     1141 ataaccgcga ttgactgttt ctactgacat tgttatcacc tccccatata ctcattatac
     1201 cgaaaatacg ttcggttttc caagcaatag tatatgggtt gcggtacatc aattcaaagt
     1261 aatcattcat ttcaagcagt catctgtcga gcttcgaggt gccaaaggta cccctaatcc
     1321 cgaacggcga ttcatctcat gactgaagtc acgagggttt tcgcctaata ccctaaaaaa
     1381 tggttgaagg tcttttgacc ttcaaccaaa ctcaattaat ggccaacttt ttgaaatttc
     1441 cctcttaaga aagtgtgggc gaactggatg tcttccagcc aattttcttg tccggtatac
     1501 cagaattcgg caacgaagcg tcggacgcct tctttccaca tcgttttgat cgtttcttcg
     1561 aaagatacat gtcccgtacc gaaggggatt tcacggaaat gacccggtat tgtttccttc
     1621 agatgtgcgg caaccatatg tcctcttccc gtttggatat cttcaaagac ggagttcgtg
     1681 caggacaagg aagcattctt caagttccct gtgtccggat aaacctgcag atacggcgat
     1741 tggatgatgt ccacatattc catcgccttc tccacagtgt tcatgaacgg tgtttccatc
     1801 gtctcgaatc ctaacagaat cccgtttttg ctcgccatat cgacggcttt ctgcagattt
     1861 ccgatgaagt attccttggt ctgctgatca ccgtcttcat aatagacatc gtaacctgcc
     1921 aattggatga tgcgtattcc caaatcatcc gccaattcga tggctttctc catgatttcc
     1981 aagctttttg cccggatgag cggatcatgc gatcccagcg gatatttccg atggccgctg
     2041 aggcacatgg agcggatcgg cagcccgact tcatacatgg cgtccacaag ctctttgcgc
     2101 tcttgcttac tccattcgat ccgtgccagt ttctcgtctg tttcatcgac actcatttcc
     2161 aagaaatcat agccggcatt tttcgcaatt gtcagctttt ctttccagct cagattgccc
     2221 ggcattgatt tttcatagat tcctaacgtg taagtcatga ggctcctcct tgacctgtcc
     2281 gtagtaggct tccggaccgt gttttcgaag aaaatgttta tccaacagtt cctgctgcat
     2341 gctttcgatc cctggattca tgacgcggtt gcgccaggac atcatggcaa cttcttccat
     2401 gacgaccata ttatggaccg cctcgaaggc gtcctttccc caagcgaagg ggccatggct
     2461 gtggaccaac acacccggaa cttggtcttc atcgatagct tcaaatgtct caacgattac
     2521 tttcccggtt tccagttcat aggatcccgc gatttcttca ctcgtcatcc ggcgggtgca
     2581 agggattgtt ccgtagaagt aatccccttg cgtcgtccca taggctggaa tctctactcc
     2641 cccttgcgcc aaagtggttg cccatggtga atgcgtatgg accactccgc cgatattttt
     2701 gaagtttctg tagagttcaa gatgcgtatc caaatccgaa gatggtttca ggctgccttc
     2761 cactacattt ccgtccatat cgacgacaac gatgtcttca acagtcatat cagcatattc
     2821 gaccccggat ggcttgattg cgatgatttg tttttcacgg tctatttcgg aaacattgcc
     2881 ccaggtgaat ttcaccagcc catattccgg cagcgacagg ttagcttcca acactctttt
     2941 tttcaattca gtgtatgtca tgggagcacc gcctattcgc ccaagacgat ttcgcggact
     3001 ttttcttcga tctctttttc agacaagaga tttttcaaac cgatgatttt cgttttgctt
     3061 tgatccacat ttttgaatac gtccaaaagc gagttcgagc aaacaaccac atcgtagttt
     3121 ggagctaacg tttttgcttc ggaaaccgcg cagtgatgga tttccaaggg tatgttcaat
     3181 tttttgaaga cattcgtaat tttcattttg atgatttggc ttgaacccat tccgcttccg
     3241 catgctgcta gtactttcat aattaattcc tcctattatg tatatccctg tttttttgat
     3301 taggcagctt cagtgctgaa ttcagctttg ttttcagctt cgatttcttc tttgtattgt
     3361 tcataatctt ccgcaatcat gaagtagtgt tttttgtttt tgtagtattg aatctgcggt
     3421 atcaacagca tcccgatgac aagtgcagcg acaccggcat aac
//
LOCUS       DAOMUJ010000161         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7749_length_3454_cov_2.429244, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000161 DAOMUJ010000000
VERSION     DAOMUJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7749_length_3454_cov_2.429244"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1336
                     /locus_tag="PLT73_05060"
     CDS             332..1336
                     /locus_tag="PLT73_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRM19782.1"
                     /translation="MGIFQWLNDQLLKMQWLSDLVGILVRDVFGLDLASRVGGSIHFF
                     IYDVIKIFILLSVLIFSISYVQSFFPPERTKKILGRFDGITANILAALLGTITPFCSC
                     SSIPLFIGFTGSGLPLGVTFSFLISSPLVDLASIILLASIFNWKIAIAYVVVGLILAV
                     VGGTFIGKAHLEDQVESFVFGSPTIELEEAELTRRDRVDYAVDQVKEVVQKVWLYVLL
                     GVGIGAAIHNWIPESVITALLGQDKWYSVLVATFVGVPMYADIFGTLPISEALVAKGV
                     GLGTVLSFMMGVTALSLPSMVMLKQVVKPKLLGLFFGIVAVGIIIIGYVFNFLGPIFM
                     "
     gene            1369..1602
                     /locus_tag="PLT73_05065"
     CDS             1369..1602
                     /locus_tag="PLT73_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM19783.1"
                     /translation="MEIKILGIGCKNCVNLAKNTEEALKELGMEATITKVTDMKDIAK
                     YGIMRTPGLVIDEKVVSYGKVASTEEITELLKK"
     gene            1779..2303
                     /locus_tag="PLT73_05070"
     CDS             1779..2303
                     /locus_tag="PLT73_05070"
                     /inference="COORDINATES: protein motif:HMM:NF018637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HRM19784.1"
                     /translation="MTNITIFEPETSSSLFSSQDAMRIAAVMQALEGLENYEALLFNL
                     TDDADQFTLNKAVKEKIEAEGNSVLPITVVDGAIVKSGNYPTNDEITSYIGVRFIEET
                     EGSCGCGGCGCGGSEEFAEAEKEEKEESGCCGGHGEKGGCGCGGQGHHHHHKHEEAAK
                     ATKGSCGCGNGGCC"
     gene            2403..2756
                     /locus_tag="PLT73_05075"
     CDS             2403..2756
                     /locus_tag="PLT73_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003582114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM19785.1"
                     /translation="MDYENYAKVAKALADPKRVKIVDMLSCGAMCACDILEHFDFTQP
                     TLSHHINLLVKAGLVTTEKSGTWHYYDLNKNAFEKFKADTAELSADTPACICEPVRSK
                     VVCAIEKRADQVTQK"
     gene            2799..>3454
                     /locus_tag="PLT73_05080"
     CDS             2799..>3454
                     /locus_tag="PLT73_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HRM19786.1"
                     /translation="MSGKEQTSISFFNKYLSVWVASCMVVGVLIGKYLPAIPEFFGKF
                     EYANVSIPTAVLIWVMIYPMMMKVDFQSVRDVGKNPKGLYVTWVVNWLIKPFSMYAIS
                     AFFFFVVFKNFITPELATEYLAGAILLGAAPCTAMVFVWSQLTKGNPAYTVVQVATND
                     LIILVAFIPIVKFLLGVSNVTVPWDTLILSVVLFVVIPLVGGVLTRIFVTKKKGVAYF
                     "
BASE COUNT          954 a          701 c          888 g          911 t
ORIGIN      
        1 aaagtggagg acccgaaaga aatcgcaaag cacggcatta tgcagactcc ggggctggtc
       61 atcaatgaaa aggtcgtctc atatgggcgt atcctgacgc ccaaacacat tgcagaactg
      121 ttgcaaaagt gacaaaaaga gaaaagccta tccttgaatg aacaagctgc gaaactgggg
      181 ttctgctcct gcattatcgc agcttttttg aagcctttca ctacccaaaa acaacacatt
      241 gacacttatc tatgtgtggg cgtatactga tatatagata aacatcaatg tcttgggtag
      301 taaaaataca aactagaagg tgcaattcgt tatgggaatt tttcaatggt taaatgatca
      361 gttattaaaa atgcagtggc taagcgattt ggttggtata cttgtccggg atgtcttcgg
      421 attggacctc gccagtaggg taggcggcag catccatttc ttcatctacg acgtcatcaa
      481 aatcttcatt ctgttgtcgg tgctgatttt ctcgatttca tacgtgcaaa gctttttccc
      541 gcctgagcgg acgaagaaaa ttctaggacg ttttgatggg atcaccgcca atattctggc
      601 ggcgttgctg gggacgatca caccattctg ttcctgttct tcgattccgc ttttcatcgg
      661 cttcaccggt tcgggactgc cgttgggtgt gacgttttcg ttcctgattt cttcaccgct
      721 tgttgacttg gcatcgatta tcctcttggc cagcatcttc aactggaaga ttgccatcgc
      781 ctatgttgtt gtgggattga tccttgcggt tgtcggcgga actttcatcg ggaaagcgca
      841 cctggaggac caggtcgaat cctttgtgtt cggtagcccg acaattgaac ttgaggaagc
      901 cgagttgaca aggagagatc gtgtcgatta tgcagttgat caggtgaagg aagtcgtcca
      961 aaaagtatgg ctttacgtgc tccttggggt cggtatcggg gcggccatcc acaactggat
     1021 tccggaatcc gtcatcacag ctctgttggg tcaggataaa tggtattcag tattggtggc
     1081 aacattcgtc ggggttccga tgtacgcgga tattttcggg acgctgccca tttccgaagc
     1141 acttgtcgca aaaggggtcg gattgggaac cgtgctttcc ttcatgatgg gcgtgacggc
     1201 attgtccttg ccgtcgatgg tcatgctgaa acaggtcgtc aaaccgaaat tgttgggcct
     1261 tttcttcgga atcgtggcag tcggcatcat catcatcggc tatgtgttca atttcttggg
     1321 acctattttt atgtgatcaa aaaaataata aacaggagga atatcatcat ggaaatcaaa
     1381 attttgggta taggctgcaa aaattgcgtg aatttggcta agaacacgga agaagcattg
     1441 aaggaactgg ggatggaagc aaccatcaca aaagtgaccg acatgaagga catcgcaaaa
     1501 tacggcatca tgcgcactcc gggattggtc atcgacgaaa aagttgtttc ctacggtaaa
     1561 gtcgccagta cggaagaaat tacagaattg ttgaaaaaat aagcaagtta ccaaaagtgc
     1621 gggaatagac acatttatcc ccgcactttt tttgtttcat gttgaattca gcattgacta
     1681 tagatagaag aaaatctata ttataagagt accaatattt aaatgaaaac aatacaagat
     1741 tcatcttcaa atagaaagag aatgaggaga tacgaaaaat gacaaacatc actatttttg
     1801 aaccggaaac aagcagcagc ttgttcagca gtcaggatgc catgcgcatt gcagcagtaa
     1861 tgcaggcctt ggaaggactg gagaattacg aggcattgct ttttaattta accgatgatg
     1921 cggatcaatt cacgctcaac aaggccgtta aagaaaaaat cgaggctgaa ggcaatagcg
     1981 tattgccgat cactgttgtg gatggagcaa tcgtgaaaag cggcaactat ccgaccaacg
     2041 atgaaatcac aagctacatc ggcgtccgct tcattgaaga aacggaaggt tcatgtggtt
     2101 gcggcggctg tggttgtggc ggttcggagg aatttgccga agcggaaaaa gaagaaaaag
     2161 aagaatccgg ctgctgcggc ggccatggcg aaaaaggcgg ctgtggctgc ggtggacagg
     2221 ggcaccatca tcaccataaa catgaggaag ctgcaaaagc aactaagggc tcgtgcggtt
     2281 gtgggaatgg cggctgttgc tagcaaaaat aaagaatact cgtgtctcgg gttacctagg
     2341 ctcgagtttc tttttccacg atagatagaa aaacttctat gtaaaaaggg gattcagcaa
     2401 tcatggatta tgaaaattat gcaaaagtcg caaaagcttt ggccgacccg aagcgcgtca
     2461 aaattgtcga tatgctttct tgcggagcca tgtgtgcctg cgacatactg gaacatttcg
     2521 attttaccca gccaacttta tcccatcaca tcaatctgtt ggtgaaggcg ggattggtga
     2581 cgacagaaaa atccggcacg tggcactatt acgatctgaa taaaaacgcc tttgaaaaat
     2641 tcaaggcgga cacagcggaa ctgtcggctg atacaccggc ctgcatctgc gaacctgtac
     2701 ggtcaaaagt ggtgtgtgca atagaaaaaa gagctgatca ggtgacgcaa aaatagagct
     2761 gtaaagagcg taaaacgata aatgggggta taataaaaat gagtggaaaa gagcaaacaa
     2821 gcatctcgtt cttcaataag tatctgagtg tctgggtagc cagttgtatg gtggtggggg
     2881 ttttgatcgg taaatatctg cccgccatac ctgaattctt cgggaaattc gaatatgcaa
     2941 acgtctcgat cccgacggcc gtgttgatct gggtcatgat ctatccgatg atgatgaagg
     3001 tggatttcca aagtgtccgg gatgtcggaa aaaatccgaa aggactctat gtcacatggg
     3061 tagtgaactg gttgatcaaa ccgttcagca tgtacgccat ttccgcgttt ttcttctttg
     3121 tcgtcttcaa gaatttcatt acgcccgagt tggcgacgga gtacttggcc ggtgcgattc
     3181 tgctgggagc tgcaccttgt acagcgatgg tgtttgtctg gagtcaattg acaaaaggca
     3241 atccggcgta caccgtcgtc caagtagcca ccaatgatct gatcatcctg gttgccttca
     3301 ttccgatcgt caaattttta ttgggcgtca gcaatgtaac ggtgccttgg gataccttga
     3361 ttttgtccgt tgtgttgttt gtcgtgattc cgttggtcgg tggtgttttg acgcggatct
     3421 tcgtcacaaa gaaaaaaggc gtcgcttatt tcga
//
LOCUS       DAOMUJ010000162         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7796_length_3437_cov_2.253105, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000162 DAOMUJ010000000
VERSION     DAOMUJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_3437_cov_2.253105"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..544
                     /locus_tag="PLT73_05085"
     CDS             182..544
                     /locus_tag="PLT73_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRM19787.1"
                     /translation="MELKMKRIYESPEETDGFRILVDRLWPRGVKKEAAQLDAWLKDS
                     APSPDLRKWFAHDPDKFAAFKERYEEELLTNPPTAAAVAEILKQLTETDVTLVYAARD
                     PEINHVVVLRDFVESQRK"
     gene            complement(825..1754)
                     /locus_tag="PLT73_05090"
     CDS             complement(825..1754)
                     /locus_tag="PLT73_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM19788.1"
                     /translation="MNDFTDPITDNNRIVASKVDGKTMPIHFRNHLEEDVLFPSNDFN
                     LTLLRQHFQHLRHDTIPLHWHKELQAVWVHEGTLAFTVNEETFPLSDDTLLLINRGQL
                     HRSRTIAGDAAAICINFEPDFFHPKVLQDYILPLLEKEAFSYRLLALSPEMTARLHRI
                     LAASDRSIPYFSVINLLSDCLEEIMQSSSGSGKTADFEDRNLFHRLLAYVEAHYQSPI
                     TVADLANHALINKNRCTALFQKYTQTSPMKYVAKHRLFLAKNLIIGTDLSISEISEAV
                     GYNQTSYFVEQFRRSYGLPPLKYRKQFGDSSKV"
     gene            1938..2870
                     /locus_tag="PLT73_05095"
     CDS             1938..2870
                     /locus_tag="PLT73_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM19789.1"
                     /translation="MSNKLKGILLAAGGGSLWGISGILAQLLFADYTVSSEWLVSTRL
                     LMAGILILFYSHAVKREGIFVIFRQKRDIIRLLLFAVFGMVACQYLFFKAIEMSSASL
                     AAILQFTAPIFVYLYMLVKKEKRFNPAEGGLVLATFAGVLLIVTKGDFSQIAVSPLGL
                     ALGIGSAIGVAFYTLQPRLILKKYGSPVVVGWGMFIGGVLFQFIHPFWAPGFAVTAQS
                     MLLTGAIILFGTAVAFLAYLESVKYIEASLASVMTALEPLLAAVLSVLVFGTSFGLFE
                     WLGIAIVLGSVLLLSNFSRFKEKSPSRECGKGIL"
     gene            complement(2930..3289)
                     /locus_tag="PLT73_05100"
     CDS             complement(2930..3289)
                     /locus_tag="PLT73_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19790.1"
                     /translation="MQMKAYLALPLVLLLMACSNGAEETPETEVTQSSVSESSEVIST
                     EVIDDETAFLEQLADFDAEHEPFIVAYYDSLDDIKEKVSDETNTFVDPKLSERELQIK
                     LVFKGDDDYYRVVLEQK"
BASE COUNT          815 a          796 c          931 g          895 t
ORIGIN      
        1 agcgcttaaa atggtaagga gaactatcaa gcgaaactgc tggccagagt agcggaagtg
       61 catcggcttg aggaagaaat cgccaatttg cagcagcacc tatacctgga gaaggtacaa
      121 gtggataaga tgatggaact ggtggcccag ttttaaagca gtaaatggga ggagaatgga
      181 catggaactg aagatgaagc gtatctacga atctcctgag gaaacagatg gcttcaggat
      241 cttggttgat cggttgtggc cgcgtggagt caagaaggag gccgcgcagt tggatgcatg
      301 gctgaaggat agcgcaccgt cgccggattt gcgcaaatgg ttcgcccacg atcccgataa
      361 attcgccgcc ttcaaggaac ggtatgaaga ggaactattg acgaacccgc cgaccgctgc
      421 tgctgtagcc gagatcctga aacagctgac agagaccgac gtcacgctgg tctacgctgc
      481 gcgggatccg gagatcaatc atgtcgtcgt gctgcgggat tttgtggaaa gccagcggaa
      541 ataagcaact atagaatgcg gaagaagccg cccttggcca agggcggctt cttccggttg
      601 ggtgtgctgc ctcagtcggc tttcggagca gcgtcagcgt ccgttcagtc gacataagtg
      661 ttttgtctcg cgtttcagtg gggatgggca tgcggaatag tcgacgctag cccaagctgt
      721 cggctttcgg agcaaccgcg cagtacaatc agccaacata catgcgtcat ctcggcttta
      781 cgaagaaaaa acagtgccga atgaccgacg ccgccgaaca cccatcacac tttactgctg
      841 tccccgaact gcttccggta tttcaaaggc ggcagcccgt agctgcgccg gaattgctcc
      901 acgaagtagc tggtctggtt gtagccgacc gcttcgctga tttcgctgat cgagaggtcg
      961 gtgccgatga tcagattttt ggccagaaag agccgatgct tggcgacgta tttcatcggg
     1021 gatgtctgag tgtacttctg gaacagtgcc gtgcagcggt ttttgttgat taaggcatgg
     1081 ttggccagat cagcaacggt gatcggactt tggtaatgtg cttctacata tgctagcagt
     1141 ctgtggaata aatttcgatc ctcaaaatcg gccgtttttc cggacccact ggacgactgc
     1201 atgatttcct ccaggcagtc cgaaagcagg ttgatgacgg aaaagtaggg gatggatcgg
     1261 tcgcttgccg ccaggatccg gtgcagccgg gccgtcattt ccggagacaa agccagcagc
     1321 cgatagctga aggcctcttt ttccaacagc ggcagaatgt agtcctgaag cacttttgga
     1381 tggaaaaaat ccggctcgaa attgatgcag atggcggctg catcgcctgc gattgtccgg
     1441 gatcggtgca gttgcccgcg gttgatgagc aggagcgtat cgtccgataa cggaaacgtc
     1501 tcctcgttca ccgtgaatgc caacgtgcct tcatggaccc agacagcctg cagttccttg
     1561 tgccaatgca gcggaatcgt atcgtggcgc agatgctgaa aatgctggcg aagcagtgtc
     1621 agattgaagt cgttggaagg gaaaaggacg tcctcttcca agtgattcct gaaatggatc
     1681 ggcatcgttt tgccatcgac tttgcttgca acgatgcggt tgttgtctgt aatgggatca
     1741 gtaaaatcgt tcatctggat cgctccttct tcgttcggtt ctgcagcttt tctgatatta
     1801 tcataacaat ttcgatcttc ctctagcaaa tccggcaata gaatttacac gaatctgata
     1861 aaaataaata ggcaattgat agaaccagtc agatcagccg actataatga atccatacag
     1921 aaatgggagc gggcaatatg agcaacaaat taaaaggaat tttattggcg gcgggcggag
     1981 gcagcctgtg ggggatctcc ggaattttgg cgcaactgct gttcgccgac tatacggtct
     2041 cgtccgaatg gctggtaagc acgcgcctcc tgatggcggg aatcctgatc cttttctata
     2101 gccacgccgt gaagcgggag ggtattttcg tgatttttcg tcaaaaaagg gacatcatcc
     2161 gccttctgtt gttcgcggtg ttcggtatgg tcgcctgcca gtatttgttt ttcaaagcaa
     2221 tcgagatgag tagcgcatca ttggctgcca ttcttcagtt tacggcacca atttttgtct
     2281 atttatatat gctggtaaag aaagagaaac gcttcaatcc ggctgaaggc ggattggttc
     2341 tggcgacttt tgcaggcgtg ctgctgatcg tgaccaaagg ggatttcagc caaatcgcgg
     2401 tttcgccgct tggcctggca ctcggcatcg ggtctgctat cggcgtggcc ttttacaccc
     2461 tgcaaccgcg cctgattctg aaaaaatacg gatcccccgt ggtcgtgggt tggggtatgt
     2521 tcatcggtgg cgtgctgttc cagttcatcc accctttttg ggcccccgga ttcgcggtga
     2581 cagcgcaaag catgctgctg accggtgcga tcatcctctt cggcacggct gtcgccttcc
     2641 tggcgtatct ggaaagcgtg aaatacatcg aagcctcctt ggcgagcgtc atgaccgcat
     2701 tggagccgtt gctggcggcg gtactttcgg tgctggtttt cggcacctca ttcgggcttt
     2761 ttgaatggtt gggcattgcg attgttttgg gatctgtact gctgctgtcg aatttcagcc
     2821 ggttcaaaga aaaaagccct tcccgggaat gcggaaaagg aatcttgtaa aaacaaagca
     2881 agcagcaggc gactctttag cgccggctgc ttgctttaaa ttactttggc tatttttgtt
     2941 ccaataccac gcggtaataa tcatcatcgc ctttgaaaac caatttgatt tgtagttccc
     3001 tttcggaaag tttcgggtcg acaaaagtat tcgtttcgtc ggatactttt tctttaatgt
     3061 cgtcgaggga atcataatag gctacaatga atggttcatg ctccgcatca aaatccgcga
     3121 gttgttccaa aaaggccgtt tcgtcgtcga tcacttccgt gctgatgact tctgaacttt
     3181 cgctgactga tgattgcgtg acttcggttt caggcgtttc ttctgcgcca ttcgaacagg
     3241 ccataagcaa caatacaagc ggaagagcta agtaggcttt catttgcaag ttgattcctc
     3301 ccatttttta attatttcaa tattaagtat aaaccagtta tgcttaatgt tgaaataatg
     3361 ttaataataa ggaacaaaaa gatttgggat aaatgaatac tccctatcgg ttatcgcttg
     3421 acttaacctt aacttta
//
LOCUS       DAOMUJ010000163         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7872_length_3416_cov_1.520083, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000163 DAOMUJ010000000
VERSION     DAOMUJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_3416_cov_1.520083"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1251)
                     /locus_tag="PLT73_05105"
     CDS             complement(<1..>1251)
                     /locus_tag="PLT73_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19791.1"
                     /translation="KGSVLLVTHDRYFLENAVTKIIELKNGGLEVYTGNYEDYLFQKS
                     EREAIQQKMDEKQLKLYKSELQWMRKGAKARTTKRIHRFEDLEKVTQQSTTDAKLEIQ
                     LDGSRLGKRVFNLEHISLFAGEKQILNDFSYIFQSNDRIGIVGKNGAGKTTLLNMLAG
                     EIAIAGGNLIIGETVKIAYYKQLSEVLPDDKRVINYLQEIAEEVKLSDGIVVSVTEML
                     ETFLFPRETHGSLISSLSGGEKRRLYLLKLLMTKPNVLLMDEPTNDLDIDTLTVLEDY
                     LNSFGGAVITVSHDRYFLDKVADKLLILNDEGKPGVFFGDMSEYLLVSRQKESEAAKQ
                     EIKKAAAPVPARDAVKEKTKWTYMEQKEWETIEDDIAELEEKGQTLQSAMAENASDFE
                     KLTQLQQELDDAEAALAEKWERWE"
     assembly_gap    1252..1284
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1285..1866)
                     /locus_tag="PLT73_05110"
     CDS             complement(<1285..1866)
                     /locus_tag="PLT73_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM19792.1"
                     /translation="MKDFNAIELEKSYGMKKLFNKISFTIREGEHIGLIGQNGTGKSS
                     LLEIIAGIETPDAGSLDVPSDYRVGYLAQEPQLNKESTVFEAVYEGEAPIIKTVRAYE
                     EALELLEKDSLNPDYQNRYSKAEADMNAQDAWQTEVQIKSILNRLGLDDITKKVGELS
                     GGQRKRVGLAQVLIQAPDLLLLDEPTNHLDMDSI"
     assembly_gap    1996..2005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2082..>3416)
                     /locus_tag="PLT73_05115"
     CDS             complement(2082..>3416)
                     /locus_tag="PLT73_05115"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM19793.1"
                     /translation="QVQPMQDINLHFTGDMHAITATNNTLSALIDNHIQQGNTLNIDQ
                     RRITWKRVLDINDRALRNIVIGLGGPIQGVPRQDGFDITVASEIMAILCLSTSLTDLK
                     NRIGNIVIGYTFDRVPVTVRDLKVEGALTLLMKDALEPNLVQTLYHTPAFVHGGPFAN
                     IAHGCNSVLATKTALKLADYVITEAGFGADLGAEKFMDIKVPQLGKSPDAVVIVATVR
                     SLKMHGGVLVKDLNSGENVEAVKAGFANLQKHIENMQQYGVPVVVALNEFTNDTAAEI
                     EAVVSLCAAQEVECIQTAVWAKGAEGGEALANTVVKAIDENTKAFVPLYVAEDSGIEE
                     KIETIVTKIYGGKSVIYSKEAKNQLRQYKKNGWDRLPVCMAKTQYSLSDDQQAIGRPE
                     NFDITIRSFVPKIGAGFVVALTGDILTMPGLPKEPAALRMDVTEDGVVTGLF"
BASE COUNT          718 a          898 c          773 g          984 t
ORIGIN      
        1 tattcccaac gttcccattt ttcggcaagc gctgcttctg cgtcgtccaa ttcctgctgc
       61 aattgggtca gcttttcgaa gtcagaagca ttttcagcca tcgcgctctg caatgtctga
      121 cctttttcct caagttcggc aatgtcgtcc tcgatcgtct cccattcctt ctgctccatg
      181 taggtccatt tggtcttttc tttgaccgca tccctagccg ggacaggcgc tgccgctttt
      241 ttgatttcct gtttagcggc ctcgctctct ttttgtctcg aaaccagcaa atattcactc
      301 atatcgccaa agaatacgcc tggtttgccc tcatcgttca gaatcaacag cttatcggcc
      361 actttatcca ggaaatagcg atcatgcgaa acggtgatga cggctccgcc gaagctgttc
      421 aagtaatcct ccaataccgt caacgtatcg atgtccaagt cgttcgtcgg ctcatccatc
      481 agcagcacat tcggtttcgt catcagcagc ttcaacaagt aaagtcgacg cttttcccct
      541 cctgataacg aactgattag gctgccgtgg gtttcgcgcg ggaagaggaa ggtctccagc
      601 atttccgtga cgctgacgac aatgccgtcg cttaatttca cctcttccgc gatttcctgc
      661 aggtaattga tgacgcgctt atcgtcagga agcacttcgg aaagctgttt gtaataggct
      721 attttgacag tctctccgat gatcagattc ccgccggcaa tcgctatttc gcctgccaac
      781 atattcagca aggttgtctt gcctgcccca ttcttgccga cgataccgat gcgatcgttc
      841 gactggaata tataggaaaa atcgttgagg atctgttttt ctcccgcaaa cagcgagata
      901 tgctccaaat tgaagacgcg tttgcccaac cgggaaccat ccaattggat ttcgagtttg
      961 gcatcggtcg tgctttgttg cgtgaccttt tccaaatcct caaatcggtg gatccgcttt
     1021 gtcgttctcg ctttggcacc cttgcgcatc cattgcagtt cgcttttgta gagtttcagc
     1081 tgcttctcat ccattttttg ttggatggct tcacgttcac tcttttggaa taaatagtct
     1141 tcgtagtttc ctgtgtagac ttccaatccg ccgtttttca gttcgatgat tttcgtgacg
     1201 gcattctcca ggaagtagcg atcatgggtc accaacagga cggagccttt gnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnaatcga gtccatgtca agatggttgg tcggttcatc
     1321 cagcaacaat agatccggcg cttggatcag cacctgcgcc aagccgacgc gtttgcgttg
     1381 cccaccggat agctcgccta cttttttcgt gatgtcgtcc aaacccaacc tgttcaggat
     1441 tgatttgatc tgaacctctg tctgccaagc gtcttgcgca ttcatgtcag cttcagcttt
     1501 actgtatcgg ttctgataat ccggattcag actgtctttc tccaacaatt ccaaagcttc
     1561 ctcgtaggcg cggaccgtct tgatgatggg cgcttcccct tcatagaccg cttcaaaaac
     1621 agtcgattct ttgttcagtt gaggctcctg cgccaagtaa ccgacacgat aatcactggg
     1681 cacatccagg ctgccggcat ccggggtttc gattcccgcg atgatctcca gcaaggaact
     1741 tttgccggtt ccgttctggc cgatcagacc gatatgttca ccttcgcgga tcgtgaagga
     1801 tattttattg aagagtttct tcatcccgta ggatttttct aactcgatgg cgttaaagtc
     1861 tttcatatgt ggcattcatc ctttgttttc aatccatgtc gttttatgtt cagggatctt
     1921 gatagtctct tgacattgta tcatatcagg acactctacg cagaagaaaa gagagggata
     1981 aaccctctct tttctnnnnn nnnnnaagcc gtatttcaga agcgcttttc atcaaaaaaa
     2041 tagagaggtt tccctctcta ctttttttaa gctttatgaa attagaacaa cccggtaacg
     2101 acaccgtctt cggtgacgtc catgcgcaaa gcagccggtt ctttcggcag acccggcatg
     2161 gtcaggatat cccccgttaa cgcaacgacg aacccggctc cgatcttcgg aacaaaggaa
     2221 cgaatggtga tgtcgaagtt ttccggacgg ccgattgctt gctgatcatc tgacaaggaa
     2281 tactgtgttt ttgccataca taccggcaaa cggtcccagc cattcttctt gtactgacgc
     2341 agttggtttt tggcttcttt ggaatagatg acacttttgc cgccataaat tttggtgacg
     2401 atcgtttcga ttttttcttc gatgccgctg tcttccgcca cataaagggg aacgaaagct
     2461 tttgtgtttt catcgattgc tttgacgaca gtgttcgcca acgcttctcc gccttcagcg
     2521 ccttttgccc aaactgctgt ctggatgcat tcgacttcct gagccgcaca cagactgacg
     2581 actgcttcga tttccgccgc ggtatcgttc gtgaattcat tgagcgccac gacgacaggc
     2641 acaccgtact gttgcatatt ttcgatatgc ttttgcagat ttgcgaagcc ggctttgacc
     2701 gcttcgacat tttcgccgct gttaaggtcc ttcaccaata cgccgccgtg catcttcaaa
     2761 gagcgcacgg tcgctacgat aacgactgca tccgggctct tgcctagttg cggtactttg
     2821 atgtccatga acttctcagc acccaagtcg gctccgaaac cggcttccgt aatgacgtag
     2881 tcagccaatt tcaaggctgt tttggttgcc aatacgctgt tgcaaccgtg cgcgatatta
     2941 gcgaacggtc cgccatgcac aaatgccggc gtatggtaca acgtttggac caagttcggc
     3001 tccaatgcat ccttcatcag caaggtcaat gcgccttcca ctttcaagtc tctgacagtg
     3061 acgggtacgc ggtcaaacgt ataaccgatg acaatgttcc cgatccggtt cttcagatcc
     3121 gtcagtgaag tcgataagca aaggatcgcc atgatttcgc tcgctaccgt aatgtcgaag
     3181 ccgtcttggc ggggaacgcc ttggatcggc ccgcccaacc cgatgacgat gttgcgcaaa
     3241 gcacggtcgt tgatgtccaa tacgcgcttc caggtgatcc ggcgttgatc gatgttcaag
     3301 gtattgcctt gttggatatg gttatcgatc aaagctgata aggtattgtt cgtcgctgtg
     3361 atggcatgca tgtcgccggt gaaatgcaga ttgatgtcct gcatcggttg cacttg
//
LOCUS       DAOMUJ010000164         3399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7925_length_3399_cov_2.221292, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000164 DAOMUJ010000000
VERSION     DAOMUJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7925_length_3399_cov_2.221292"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1006)
                     /locus_tag="PLT73_05120"
     CDS             complement(194..1006)
                     /locus_tag="PLT73_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM19794.1"
                     /translation="MKSSQGIVYKRRQHILKALQEKKVVLVEDLAKELGVSEITVRRD
                     LQSFDDQHLIERFYGGARLLEGSFQKEEYPAVKPHRSKKQEIAKLAATLVHDHDMIFI
                     NSGSTAFLLLNYLSDRNVTIITNNGRAIFRNPSSKAEIVLSGGEIYEKKKSLVGDFAL
                     YTFSKVTANICFLGVGGINEKGITTYALPETAVNRLILERTNGPRIVVAEGSKVGREQ
                     NFSTASISLITHLVTDRSADKEEIKKIEAKGVKVLYVTEVNVNGENEIRIEE"
     gene            complement(1293..>3399)
                     /locus_tag="PLT73_05125"
     CDS             complement(1293..>3399)
                     /locus_tag="PLT73_05125"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRM19795.1"
                     /translation="SSIYDGEVYDANLEIDGWNKYSESESAVCWERMQLSDTDTEELQ
                     ARLSVPVKIVDAFVPELIITPAGEQVLDFKQNMTGWITFSADVPDGTVITYEVGEVLQ
                     EDNFYRDNLRTAEAKFTYISNGKAAEVRPYFTFYGFRYAKITGVDLLEQDLGFKGYVV
                     QSELEETSGMITANPDVNQLLKNALWGQKGNFLDVPTDCPQRDERMGWTGDAQIFART
                     ASYNMYTPAFYKKFMHDLRLEQKALNGSVPFVVPQIKPEGDAGFVTGSGAAAWSDAAT
                     VIPWVLYEQYGDRSLLKEHYPTMKDWVDYIVREDTKSGDKRLWTSGFQFGDWLALDGD
                     NPHSPMGGTDTALVASVYYYHSASLLARAAEVLGNTHDALAYTQLALEVKQAIQNEYL
                     TTTGRLAVTTQTAYILFHYFDLCPEPFKERMTKDFYDKVISSGVHLQTGFVGTPYFAE
                     ALSKNGMNELAYGLLLNEDYPSWLFAVKMGATTIWERWNSILPDGKISGTDMNSLNHY
                     AYGSIVEWLYRCVLGIQADSSQPGYKHFFLAPQPSAQLGKAEGYYQSVSGRIESAWEI
                     LADGQLDFAFRVPFGTIATITLPDLTLEEAQKTEWLQSAVALEDSAKVTVKAGEYRVT
                     YMPTRNYCPEVTTEMSYRELLEYTPARKVIFEQLPMMRNNDVVLQYLDKPMENFKDLP
                     IVSNFVSEQVLEQMNASFA"
BASE COUNT          794 a          908 c          765 g          932 t
ORIGIN      
        1 cacaataggt cctatacaaa caatctttag cttattttta tgatcgtttc attggttatg
       61 aacgattcaa acgccggtgt tcttctttat gatcgcttca atgaagcgtg catgctgatt
      121 ccaacgaaaa agcgacaaaa cactgccggg gatcccgggc gtgttttgtc gctgtatttg
      181 tattctatta tattcactct tcgattctga tttcgttctc accgttgacg ttgacttcgg
      241 taacgtacag taccttgacg cccttggctt cgatcttctt tatttcttct ttatccgcag
      301 agcggtcagt gactagatgc gtgatgaggc tgatactggc ggtggaaaag ttttgttccc
      361 gtcccacttt gctgccttcc gccactacta tgcgcggccc gttcgtccgc tccagaatca
      421 gcctgttgac tgccgtttcc ggcaaggcat aggtcgtgat ccctttttcg ttgatgccgc
      481 ccacgcccaa aaaacagata ttggcagtca ccttcgagaa tgtgtacaag gcgaaatccc
      541 caacgagaga ttttttcttt tcgtatattt ccccgcccga cagcacgatt tccgccttcg
      601 aggacggatt ccgaaagatt gcccgcccgt tgttggttat gatggtgaca ttccggtccg
      661 acaaatagtt gagcaagagg aatgcggtcg atcccgaatt gatgaaaatc atatcatgat
      721 catggaccaa ggtggcagcc aacttcgcga tttcctgttt ttttgaacga tgtggcttga
      781 ctgccgggta ctcttctttt tgaaaagatc cctccagcaa acgcgcgcct ccataaaagc
      841 gctcgatcag atgctggtca tcgaaactct gcagatcccg cctgacggta atttcagaga
      901 cacctagctc tttcgccaaa tcttccacca acacaacttt cttttcttga agtgccttca
      961 gaatgtgctg ccttctcttg tagacgatcc cttgacttga cttcatgtat tcaccccgat
     1021 tcttttcttt cattgtactt gaaacaatcg gaaaaacaaa ataaaaatca ccaaacggac
     1081 tcaaaatgat cattttccac aaaaatacaa accagactca cgaccatttt caaaatcgcg
     1141 atgaagagat attgctgccc acagataaca gcagtcatct ccataaaaaa agccgaactt
     1201 ggatactcgg cttttttcct ctatcgtatt cactcttctg ctgattgtgt aagctgatga
     1261 agaatcagcg cattcttaag tcattatacc catcaggcaa atgatgcatt catctgctcc
     1321 aggacttgtt cgctgacaaa attggaaaca atcggcaggt ccttgaagtt ttccatcggc
     1381 ttatccagat actgcaagac cacatcattg ttgcgcatca taggcaattg ttcgaagatc
     1441 acttttcggg ctggtgtata ttccaatagt tcccggtagg acatctcggt tgtgacttca
     1501 gggcaatagt tccgtgtcgg catgtacgtg acgcggtatt cacctgcttt gactgtgact
     1561 tttgcgctat cctctaaagc gaccgcagac tgcagccact ctgttttctg tgcttcttca
     1621 agcgtcagat ccggcagggt gatggttgcg atcgtcccga aaggcacgcg gaaagcgaaa
     1681 tccaattgtc cgtccgctaa aatttcccac gcactttcga tgcgtccgga taccgattga
     1741 tagtaccctt ccgctttgcc caactgcgca ctcggttgcg gtgccaggaa gaagtgtttg
     1801 tatcctggct gactgctgtc cgcctggatg cccagcacgc aacggtacag ccactccacg
     1861 atcgagccat aagcatagtg gttcaaggag ttcatgtcgg tcccggagat tttcccgtcc
     1921 ggcaaaattg aattccagcg ttcccaaatg gtggtcgcac ccattttcac cgcaaacaac
     1981 cagctcggat agtcctcatt cagcaataaa ccatacgcca gttcgttcat gccgttctta
     2041 ctcaaagctt ccgcaaaata cggcgtcccg acgaacccgg tctgaaggtg gacgccgctg
     2101 gaaatgactt tatcatagaa atctttcgtc atccgttctt tgaatggttc cgggcagaga
     2161 tcgaaatagt ggaagaggat gtaggctgtc tgggtcgtta ccgccaagcg tcccgtagta
     2221 gtcaggtatt cattttggat ggcttgtttc acttccaagg ccaattgggt gtacgccaac
     2281 gcatcatgcg tattgcccaa aacctccgcg gcccgtgcca gcaaggaagc cgaatgatag
     2341 tagtacacgg atgcaaccag agcagtgtcg gtgccgccca tcggtgagtg cggattatcc
     2401 ccgtccaacg ccaaccaatc accgaactgg aacccgcttg tccagaggcg tttgtctccg
     2461 gatttcgtat cttcgcgaac gatatagtcc acccaatctt tcatggtcgg atagtgttcc
     2521 ttcagcaacg aacgatcgcc gtattgttcg taaagcaccc agggaatgac tgtcgccgca
     2581 tccgaccagg cggctgcgcc ggatccggta acgaatcctg catctccttc cggcttgatc
     2641 tgtggaacaa caaatggaac ggatccattg agcgcttttt gctccaaacg gagatcgtgc
     2701 atgaattttt tgtagaaagc cggagtgtac atattatagc tcgctgtgcg ggcaaaaatc
     2761 tgcgcatcac cagtccagcc catgcgctca tccctttgag gacaatccgt cggcacatcc
     2821 aggaaattac ctttttgccc ccaaagcgca ttcttcagca attggttcac atccggattg
     2881 gctgtgatca tgccgctcgt ctcttccagt tcgctctgga ccacgtatcc tttgaagcct
     2941 agatcctgtt ccaggagatc gacaccggtt attttggcgt agcggaagcc gtaaaatgta
     3001 aagtacggac gcacttcggc cgctttacca ttgctgatgt aggtgaattt cgcttccgcc
     3061 gtgcgtagat tatcgcggta gaagttgtct tcctgcagca cttcgccgac ttcataggtg
     3121 atgacggttc catccggcac atccgcggag aacgtgatcc atccggtcat gttctgtttg
     3181 aagtccaata cttgctctcc tgcaggggta ataatcagtt ctggcacgaa ggcatccacg
     3241 attttaaccg gaacactcaa ccgtgcctga agttcttcgg tatccgtatc gctcagttgc
     3301 atccgttccc agcaaacagc tgattctgat tcgctgtatt tgttccaacc gtcgatttcc
     3361 aggttcgcgt catacacttc cccgtcgtat atgctactg
//
LOCUS       DAOMUJ010000165         3395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7936_length_3395_cov_1.807784, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000165 DAOMUJ010000000
VERSION     DAOMUJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_3395_cov_1.807784"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..342)
                     /locus_tag="PLT73_05130"
     CDS             complement(22..342)
                     /locus_tag="PLT73_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19796.1"
                     /translation="MPITIFYLFLSQMMLFGIIRVYENQLYLYRLTENHYKAQTLLAY
                     TDYWLKEHNKISSPENRIVPASLYFEEGIVHCVADASGKVTATVTLQNAYSETLVLEI
                     LSPE"
     gene            complement(371..844)
                     /gene="comGF"
                     /locus_tag="PLT73_05135"
     CDS             complement(371..844)
                     /gene="comGF"
                     /locus_tag="PLT73_05135"
                     /inference="COORDINATES: protein motif:HMM:NF041002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HRM19797.1"
                     /translation="MPTKSMQKNPEQSQAGFTLLEAAFALTVNTLVLLLIVGGMGVIR
                     TANVQLEQRQEAEWHLFLIQLEHELEDELMVQKTNTTVRTKSKNPADTAVYTYEFRIN
                     KVLRYKNGEGYHPMLLQVKALKYEVTNEGISIQATLSDDQTRRAHLILPKAPIKQ"
     gene            complement(813..1154)
                     /locus_tag="PLT73_05140"
     CDS             complement(813..1154)
                     /locus_tag="PLT73_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRM19798.1"
                     /translation="MKSIKLNDSSGYMLFESLIALAMVSISIYVLMPHSVQFFTTLKA
                     AGAEVAYWRVAQDQMQVIAKGGNPIESQASGGILFTTTWNPETSELIVSGDDGPERVT
                     VHADEIDAKEP"
     gene            complement(1129..1572)
                     /gene="comGD"
                     /locus_tag="PLT73_05145"
     CDS             complement(1129..1572)
                     /gene="comGD"
                     /locus_tag="PLT73_05145"
                     /inference="COORDINATES: protein motif:HMM:NF040982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HRM19799.1"
                     /translation="MYKSGFTLLESLLVLMIASMILLLTPQVETATDELAFNLFLDDF
                     LTELHTAQSYAVITGNGVNMDVLRPIGATHYAVFKDGTGSDRYINRKLLLPEGSKVIQ
                     GYTGFIKGGSGYLQPNTIIMETARYRYTITIQLGSGRYEVNKAKR"
     gene            complement(1593..1913)
                     /gene="comGC"
                     /locus_tag="PLT73_05150"
     CDS             complement(1593..1913)
                     /gene="comGC"
                     /locus_tag="PLT73_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HRM19800.1"
                     /translation="MKSIKKTLKNKEAFTLMEMVLVLFIISVLMLMIIPNVANTKTSV
                     ETKGTDALAVVVQTQADMYELDTGTEAASFTVLGDGGYLNDNQVTQATAKLTIAGGNV
                     SIIE"
     gene            complement(1913..2986)
                     /gene="comGB"
                     /locus_tag="PLT73_05155"
     CDS             complement(1913..2986)
                     /gene="comGB"
                     /locus_tag="PLT73_05155"
                     /inference="COORDINATES: protein motif:HMM:NF041012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HRM19801.1"
                     /translation="MRVDTLRKKITWRISSEKRWLAKEQAYFLRRLGELLKEGFSLSE
                     GLAFLKVMQPKRSGDIQRMLVKLETGINLADALAGCGFSEQVVSQLRLSFLHGKLTET
                     LLFCSDFLTEKDKQLHKLRKVLIYPLFLLVFANVMLIAIRQVLLPSLETMLVPSQEGS
                     GLLVRIILLYLENLPLIILGTLALAASLVLLAKSYLKKKSAFQKSLLFCRIPLFSKWV
                     QLYYSFFFCREYAYFFRNGMQLNQIVSLMRGDEGTAWMKDISGLVEEGLMQGDSLTTI
                     IKRYPVFKEEMGWIIYHGELTSQLPIKLSMYSEECFRRLIADIEQKIGWLQPVLFLMV
                     AVIVMAIYLILLMPMLSSLGGMY"
     gene            complement(2937..>3395)
                     /locus_tag="PLT73_05160"
     CDS             complement(2937..>3395)
                     /locus_tag="PLT73_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM19802.1"
                     /translation="LLIRQCLRHHPDILVIGEIRDQETAKMVVRSALTGHLVIATIHA
                     KESFGVLERLRELAISSEQIKQTLLAVVSQRLIAKYCPLCSGKCTIHCAHYHVNEKSA
                     AIYEILAGKELQNHLQNKEDGKRFVTLNDKLRKAYACGYITEKNYLENLV"
BASE COUNT          855 a          859 c          745 g          936 t
ORIGIN      
        1 tatgaccttt tattttgcat atcactccgg ggacaggatt tccaatacca gcgtttcgct
       61 gtaggcattt tgcagggtca cggtggcagt gactttgccg ctcgcatccg ccacgcagtg
      121 cacgattcct tcttcaaaat acagggaagc cggtacgatc cgattttccg gactcgatat
      181 tttgttatgt tccttcagcc agtaatctgt atacgccagt aaagtttgcg ctttgtagtg
      241 gttttccgtc aatctgtaca ggtagagctg attctcataa actcttatga ttccgaaaag
      301 catcatttgt gaaaggaaca agtaaaaaat ggtgatcggc aaaatcgagc ctccccttcc
      361 tagtgatcga tcactgttta atgggggctt tcggcaggat gagatgcgct ctgcgcgtct
      421 ggtcgtccga aagcgtagcc tggatagaaa tcccttcatt ggtcacctcg tatttcaacg
      481 ctttgacttg cagcagcatc ggatgatagc cttctccgtt tttgtaacga agcactttat
      541 tgatacggaa ctcataagta taaacagcag tatcagcagg attttttgat ttggtgcgga
      601 cggtcgtatt tgtcttttgc accatcaact cgtcttccaa ctcatgctcg agttggatca
      661 ggaaaagatg ccattctgct tcttgtctct gctccagctg tacgttcgca gtgcggatta
      721 ctcccatgcc tccaactatc agcaggagca ccaacgtatt gacagtcagc gcaaacgccg
      781 cttccaataa agtgaatccg gcttgcgatt gttcagggtt cttttgcatc gatttcgtcg
      841 gcatgcaccg tcaccctttc tggcccatca tcaccgctca ctataagttc tgaggtttcc
      901 ggattccaag tagtggtgaa cagtatacct ccggaagcct gactttcgat tgggtttcct
      961 cctttggcga tgacctgcat ctggtcctgc gcgacccgcc agtaggcaac ttccgctccc
     1021 gccgctttta aagtcgtgaa aaattgaacg gaatgcggca tcagcacata gattgagatg
     1081 ctgaccatgg ccaaagcgat cagactttcg aaaagcatat atccgctgct atcgtttagc
     1141 tttattgact tcataacgac cgctccccaa ttggattgtg atggtatagc gatatcgggc
     1201 cgtttccatg atgatggtat tcggttgcag atatccactg cctcctttga tgaatccggt
     1261 gtagccttga atcacttttg atccttcagg caaaagcaac ttccgattga tataacggtc
     1321 cgatccggta ccgtctttaa aaacagcata atgcgttgcg ccgattggtc gcaggacatc
     1381 catattgaca ccattccctg tgatgaccgc ataactctga gctgtgtgaa gttccgtcaa
     1441 gaaatcgtcc aaaaacagat tgaacgcgag ttcatccgtt gcggtttcaa cttgcggggt
     1501 cagcagcaaa atcatggagg cgatcatcag taccaatagg ctctccaata atgtgaatcc
     1561 gcttttatac attattcaag cctttattca aatcactcga ttatggaaac attaccgcct
     1621 gctattgtga gcttggcggt tgcttgggtc acttgattat cgtttaggta accaccatcc
     1681 ccaaggacgg taaagctggc cgcttccgtc cctgtatcca attcatacat gtcagcctgt
     1741 gtctgcacca caaccgccaa agcatccgtg cctttagtct ctacactagt tttcgtgttc
     1801 gccacattcg ggatgatcat cagcatcagc acagaaataa tgaaaaggac gagtaccatt
     1861 tccataagtg taaaagcttc tttatttttt agtgtttttt ttattgattt cattaataca
     1921 ttcctcctaa tgaacttagc atcggcatca acaatatgag atagatcgcc atcacgatga
     1981 cggcgaccat gaggaacaag acaggctgca accagccgat tttctgttcg atatccgcga
     2041 tcagccgccg aaaacattcc tcgctgtaca tgctcaattt gatcggcaat tggctggtta
     2101 gctcgccgtg atagatgatc cagcccatct cctctttgaa aacgggataa cgtttgatga
     2161 ttgtcgtcag gctatcgccc tgcatcaacc cttcctccac caatccggat atgtccttca
     2221 tccaggcagt cccttcatcg cctcgcatca gcgaaacgat ctggttcagc tgcatgccgt
     2281 tgcggaagaa ataagcgtat tcccggcaga aaaaaaagga atagtagagc tgtacccatt
     2341 tgctgaatag cggaatccgg cagaacagca aagatttttg gaaagctgat ttctttttca
     2401 aataactttt tgccaacagg actaagctgg cggctaaggc tagcgtaccc agtatgatga
     2461 gcggcaggtt ctccaggtaa agcaggatga tgcggaccag caagccggaa ccctcttgcg
     2521 agggcaccag catcgtctcc aaactgggca gtaggacctg tctgatcgca atcagcatta
     2581 cgttcgcgaa caccagcaaa aacaaggggt agatcaagac ttttctgagc ttatgcagtt
     2641 gtttatcctt ttcagtcagg aaatcggaac aaaacagcaa ggtttccgtc aattttccgt
     2701 gaaggaagga taagcgcagc tgcgatacga cttgttccga aaatccgcat ccagccaatg
     2761 catccgcaag attgatacct gtttccagtt tgaccaacat gcgctggata tcgcccgagc
     2821 gcttcggctg catcaccttc agaaaggcaa gcccttccga caacgaaaaa ccttccttca
     2881 gtaattcacc taagcgcctc aaaaaataag cctgctcttt tgccagccaa cgtttttcag
     2941 acgagattct ccaagtaatt tttttccgta atgtatccac acgcataggc ttttcgcaat
     3001 ttatcattca gtgtgacaaa ccgctttccg tcctccttgt tctgcagatg gttctgcagt
     3061 tccttacctg ccaaaatttc atagatagca gccgactttt cgtttacatg ataatgggcg
     3121 cagtgaatgg tgcacttacc ggagcacagt ggacaatatt ttgcgatcaa acgttgggaa
     3181 acgacagcga gcaacgtctg ctttatttgt tcggaagaaa tcgccaattc ccgcagcctt
     3241 tccaataccc cgaatgactc tttggcatgg attgtcgcaa tcaccaaatg ccccgtcaat
     3301 gcgcttctta cgaccatttt agctgtttcc tgatcccgga tttcgcctat gacgagaata
     3361 tccggatgat gacgcaggca ctggcggatg agcag
//
LOCUS       DAOMUJ010000166         3393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7946_length_3393_cov_1.380767, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000166 DAOMUJ010000000
VERSION     DAOMUJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7946_length_3393_cov_1.380767"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..544)
                     /locus_tag="PLT73_05165"
     CDS             complement(320..544)
                     /locus_tag="PLT73_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19803.1"
                     /translation="MAFIVLMAIKMVGLGAAIRLPLPTPALALMGLVGFIMGLIAIIK
                     YKDRALLTLLSIPVGLVIILWTLAEIAFPH"
     gene            complement(547..807)
                     /locus_tag="PLT73_05170"
     CDS             complement(547..807)
                     /locus_tag="PLT73_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19804.1"
                     /translation="MLGAIIGDIIGSRFEWHNHRDKHFERFTEACRMTDDSIMTLVVA
                     KALMETKKTCQMQHSIFQKNKEETIMKMQFWSKQKPDNGQRV"
     gene            complement(938..1969)
                     /locus_tag="PLT73_05175"
     CDS             complement(938..1969)
                     /locus_tag="PLT73_05175"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRM19805.1"
                     /translation="MKTSIFSSNKLFKWGTIIGLAILVVVAVMFILSALGITSDFKVK
                     ETLTMEDAGVLENGVLNNGWIAETQEYIFYIDPGAGEMSPGSPVGSLIRRNRDWSGRV
                     ELTDTPVSYLSATEDWVYYSDRADNSHLYRMKADGTEKSLVISETVYSCALADDAIYY
                     TTLEGLFRSDLDGTEQKQLQSEGGNPTVVGDWVYYAEDKQSLSRIKTDGSMNQQLLSD
                     YEDYYLNEESIFYIMLTETEDESLFTLTLHQMDDTGAEATEILSIEDVVTSQLDGGYL
                     YYQLGKGQGRLEEGLYRVNLDGSGTERVNDIMIWQLDHILGDWMYILQYSGERYRIKL
                     DGSAAVLFE"
     gene            complement(2162..>3393)
                     /locus_tag="PLT73_05180"
     CDS             complement(2162..>3393)
                     /locus_tag="PLT73_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19806.1"
                     /translation="QEIKQVAKLIRRIVNKYAGRGGFIAYGEISDYVEEMVGILEKAR
                     HTADRLLALEIIILVLHEAMEAFQYADDSDGEIGGLASDCIEVMENLVLAEKKSDSSI
                     TLKLFNRLLEDSNDHAYEGWEEFTIDILRLCAEIADTEASQVLLRARVMAYLDKSDTD
                     GFSTYYAAPLLQILHRLIVVYGTHAEALQFEEENSTHASFREMLIERAKKENNFEKVI
                     ALAIDGEKQDDYYSGRKPKWKEIRYEAYKKLSLKAEQARLAKELLFDGQFEYYQELKD
                     LNNGDEKEFYDELKSKLKEDTRWQAKNIYINLIEQEDDTDEIMAYVRENPHYIVRYAE
                     LLKDNYADEVDELYSNHIRSAAHSSSKRSAYQDVCGLIRRYKNIVGKDNTAELVNELL
                     VLYKKKRAFVYELSKLD"
BASE COUNT          845 a          885 c          678 g          985 t
ORIGIN      
        1 tgtttcctag ataggagcga agttctgaca tgaatgtcag gatccctttg taggctttct
       61 gaatatgccc tttgcttaac tgtgaagcat attccttgac acattcgttt atcgattcca
      121 aaaattccac ctcattccct gattaagtat cccgcaaatt gatagagcca actatcccct
      181 ttttgtaacc gctatttccc aacctcatta taacgcgaat accacgtaga tgccctacac
      241 aagacaatgc aaatggcaga taaagaagac gccctgacta aggacgtctt tacacaattg
      301 atattcgggc aacgatgcgt cagtgcggga aagcaatttc agcgagtgtc cataaaataa
      361 tcacaagtcc gaccggaatg gacagcaacg tcaacagggc tctgtccttg tatttgataa
      421 tggcaatcag ccccatgatg aagccgacca gtcccatcaa cgcaagagca ggtgtaggca
      481 gaggcaatct gattgcagca ccaagcccga ccattttaat ggccatcagc acgatgaatg
      541 ccatagtcaa acccgctgcc cattgtccgg cttttgtttg gaccaaaact gcattttcat
      601 gatagtttcc tccttattct tctgaaagat agaatgctgc atctggcagg ttttcttcgt
      661 ttccatcagc gccttggcta ccactagtgt catgatgctg tcgtccgtca tccggcacgc
      721 ttccgtgaac cgttcaaaat gcttgtccct gtgattatgc cactcgaaac gggagccgat
      781 aatgtccccg atgattgcgc ccaacatacg aaatcacccc tttttgaaat cacgccagaa
      841 tcctcaaaag tttcagctta gctcagacta atctttaagt ccttgaaaaa ttctccgtgc
      901 ccttttcaac ccgaagcgcg ttggcgcctt cgagttgtca ttcaaacaat acagccgccg
      961 agccatccag cttgatgcgg tagcgttcac cgctgtattg gagaatgtac atccaatcgc
     1021 ccaagatatg gtccagttgc cagatcatga tgtcatttac cctttcagtt cccgaaccgt
     1081 ccaaattgac gcgatacaaa ccttcttcca gtctaccctg gccttttccc aactgataat
     1141 acaggtagcc gccgtccaac tgcgacgtta caacatcttc tatagacaga atttcagttg
     1201 cttccgcacc agtatcgtcc atttgatgca gcgtcaaagt gaataggctc tcgtcttccg
     1261 tttcggtcag catgatgtaa aagatgctct cttcattcaa gtagtaatct tcataatccg
     1321 acagcagctg ctgattcatg ctgccgtccg tcttgatgcg tgataaggac tgcttgtctt
     1381 cggcataata gacccaatcg ccgacaacag taggatttcc gccttctgac tggagctgtt
     1441 tttgttcggt accatccaaa tcgcttcgga acagcccctc gagggtagtg taatagatcg
     1501 catcgtctgc aagcgcacag gaatataccg tctcggagat aaccaagctt ttttcggttc
     1561 cgtccgcctt catccggtaa agatgactat tgtcggctct atccgaatag tagacccagt
     1621 cttccgttgc cgatagatag ctgacaggag tatccgtcag ttcgacgcgg ccgctccaat
     1681 cccggttcct tctgatcaga ctgcctaccg gggaacccgg ggacatttca ccggcgccgg
     1741 ggtcgatata aaagatgtac tcttgggttt ccgcaatcca gccgttgttc agcactccgt
     1801 tttcaagcac acccgcatct tccatcgtca atgtttcttt caccttgaag tcagaagtga
     1861 tcccgagtgc agacaaaata aacatgacag ctactaccac aaggatcgcc aaaccgatga
     1921 tcgtacccca cttgaacagt ttgttgctgg aaaaaatgct ggttttcatt aggattcctc
     1981 cattcggcta ttccatcaga gttaaagcca cgttgcgatc aacaaaaaac gttttcattc
     2041 ccatcttcat tttaccactt tctgaatcaa aaaaactccc ctaaatagtt cttggagagt
     2101 taggggagtt cgatcttttt ctaaaaagtt gctggtggaa atagcttgga ttcagaacaa
     2161 attaatccag cttgctcagc tcatacacaa aggcgcgctt tttcttgtaa aggacaagta
     2221 gttcgttcac caattctgcg gtattgtctt ttccgacgat gtttttgtat cgtctgatga
     2281 gaccgcagac atcttggtag gcacttcttt ttgaggatga atgggcagcg gatcttatgt
     2341 gattcgaata gagctcatcc acttcatccg cataattgtc tttcaaaagc tctgcatacc
     2401 tcacgatgta atgaggattc tccctgacgt acgccatgat ttcatccgtg tcatcttcct
     2461 gttcaatgag atttatatag atgttcttcg cttgccaacg ggtatcctct ttcaattttg
     2521 acttcaactc atcatagaat tctttttcgt ccccattgtt cagatccttg agttcctgat
     2581 agtattcaaa ctggccgtca aagagcagtt ccttggctaa ccgcgcctgt tcagccttta
     2641 aggataattt tttgtaggct tcatagcgga tttctttcca tttaggcttt cttcccgaat
     2701 aataatcatc ttgtttttcc ccatctattg ccaatgcgat gactttttcg aagttgtttt
     2761 cctttttggc tctctcgatc aacatttccc ggaaggatgc gtgtgtgctg ttctcctcct
     2821 cgaattgtaa cgcctcagca tgcgttccat agacaacaat caacctgtgc aggatttgca
     2881 gcaagggcgc tgcataatag gtgctgaaac catcagtgtc agatttgtcc agataggcca
     2941 ttactctcgc cctgagcagt acttgcgacg cttccgtatc cgcgatttca gcgcacaatc
     3001 ggagaatatc gattgtgaat tcttcccaac cttcataagc gtggtcattg ctgtcttcca
     3061 acagtctgtt gaacaacttc agcgttatag agctgtcgga ttttttttct gcgaggacaa
     3121 gattttccat cacctcaata cagtccgaag ccaaaccacc gatttctcca tcggaatcat
     3181 cagcgtattg gaaagcttcc attgcctcat gcaggaccag gataatgatt tccaaggcca
     3241 gtaagcgatc cgctgtatgc ctggcttttt ccaaaatgcc gaccatctct tccacataat
     3301 cgctgatttc cccataggct ataaacccac ctctgccggc atacttattc acgatacgtc
     3361 ggataagctt ggcaacctgt ttaatttcct gtt
//
LOCUS       DAOMUJ010000167         3391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7954_length_3391_cov_2.130096, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000167 DAOMUJ010000000
VERSION     DAOMUJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7954_length_3391_cov_2.130096"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PLT73_05185"
     CDS             <1..1302
                     /locus_tag="PLT73_05185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM19807.1"
                     /translation="IQDGSYTLDETAYVVETENGPKEMTMLQRWPVRRGRPFKQKMNP
                     HTPMVTGQRVIDTFFPITKGGAAAVPGPFGAGKTVVQHQIAKYSDVDIVIYVGCGERG
                     NEMTDVLNEFPELIDPNTGESLMERTILIANTSNMPVAAREASIYTGITIAEYFRDMG
                     YSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRVAEYYERAGRVLALGSED
                     REGSITAIGAVSPPGGDTSEPVTQNTLRVVKVFWALDATLAQKRHFPSMNWLTSYSLY
                     NTEVGEYLNNALQTKWSEMVQHAMNLLQEESELEEIVRLVGIESLSEKDRMTMAIAES
                     LREDYLQQNAFDDVDTYTSREKQLAMLDMILSFETEAREAMRLGAYFNEIIEGTVELR
                     DRIARSKYIKEDNLAEMENIKADIKKRLHDIVAEGGMTRHA"
     gene            1295..2671
                     /locus_tag="PLT73_05190"
     CDS             1295..2671
                     /locus_tag="PLT73_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRM19808.1"
                     /translation="MLKEYKTVTEVVGPLMVVEQIEGVKFDELVEIQMQDGEIRHGQV
                     LEVSKDKAMVQIFEGPSGINIKDTKVRFRGKPLSIAVSEDMVGRVFDGMGNPIDGGPE
                     IIPEMSLDINGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLNSDEKFAVVFAAMGITFEEKEYFMEDFRKTGAIDRSVMFINLANDPA
                     IERIATPKMALTVAEYLAYEKDMHVLVIMTDMTNYCEALREISAARREVPGRRGYPGY
                     LYTNLSTLYERAGRIIGKKGSVTQIPILSMPEDDITHPIPDLTGYITEGQIILSRELN
                     NSGIKPPINVLPSLSRLKDKGTGEGKTRKDHAPTMNQLFAAYAKGKQAKELAVILGES
                     ALSKTDKLYVQFTKRFEEEYISQGFYTNRTIEETLALGWELLSILPVTELKRIKSDMI
                     DEFMPKGE"
     gene            2678..3313
                     /locus_tag="PLT73_05195"
     CDS             2678..3313
                     /locus_tag="PLT73_05195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRM19809.1"
                     /translation="MARLNVKPTRMELSNLKSRLVLSTRGHKLLKDKQDELMRQFINL
                     IRENNILRDEVEKELTASMRSFVVAKSLLNEAFIEELFAVPATAVELDIQENNIMSVV
                     VPQMNFSMVDQDDKSTDLQYGYVNSNGELDDAIKKIEDILPKLLKLTEIEKTCQLLAD
                     EIEKTRRRVNALEYKMIPQLQETIRYIQMKLEENERSSIVRMMKVKDMGVS"
BASE COUNT          997 a          722 c          864 g          808 t
ORIGIN      
        1 attcaggacg gcagttacac gttggatgaa acggcttatg tcgttgaaac cgaaaatggc
       61 ccgaaagaaa tgactatgct gcaaagatgg ccggtacgac gcggccgtcc tttcaaacaa
      121 aaaatgaatc cacacacacc aatggttacc ggacaacggg tcatcgacac ctttttcccg
      181 atcactaagg gtggagcagc agctgttcca gggcctttcg gagcaggaaa aaccgttgta
      241 cagcaccaaa tcgcaaaata cagtgatgtg gatatcgtca tttacgtcgg ctgcggtgaa
      301 cgcggtaatg agatgacgga cgtattgaat gaatttccgg aattgatcga tccgaacaca
      361 ggtgagtcat tgatggaacg aacaatcctt atcgccaaca cctccaatat gccagttgcg
      421 gcgcgtgaag catcgattta tacaggcatt acgatcgcgg aatatttccg tgatatgggt
      481 tacagtgtag cgatcatggc tgattcgaca tcccgttggg cggaggcctt gcgtgaaatg
      541 tcaggccgtc tggaagagat gcctggtgac gaagggtatc cagcctactt aggaagccga
      601 gtagctgagt attatgaacg ggcagggcga gtattggctt taggatcgga agatcgtgaa
      661 ggaagcatta cggccattgg agcggtatcg cctcctggtg gtgacacctc tgagccggta
      721 acgcaaaata cattgcgcgt cgtcaaagta ttctgggcgc tcgatgccac tttagcacaa
      781 aaaagacatt tcccatccat gaactggttg acttcctatt ccctttacaa taccgaagtc
      841 ggggaatacc tgaataatgc cttacagaca aaatggtctg aaatggttca gcatgcgatg
      901 aaccttctgc aggaagagtc ggaattggaa gaaatcgtcc gcctggtcgg tatcgaatcc
      961 ttgtccgaga aagaccgtat gacgatggca atcgcggaat cgttgcgtga ggattacctc
     1021 cagcagaacg ctttcgatga tgtggatacg tatacttccc gcgagaaaca attggcgatg
     1081 ctggatatga ttctgagctt cgaaacagaa gcacgtgaag caatgcgttt aggcgcgtac
     1141 ttcaatgaaa tcattgaagg tacagttgaa ttgcgtgatc ggattgctag aagtaaatac
     1201 atcaaggaag ataatcttgc cgaaatggaa aacatcaagg cggatatcaa aaaaagattg
     1261 catgatattg ttgcggaagg agggatgacg cgccatgctt aaggaatata aaacagtaac
     1321 cgaagttgta ggcccgttga tggttgttga gcagatcgag ggagtcaaat tcgacgagtt
     1381 ggttgaaata caaatgcaag acggcgaaat ccgtcatggt caagttttgg aagtcagcaa
     1441 agataaagcg atggtgcaaa tctttgaagg cccaagcggt atcaacatta aagacacgaa
     1501 agtccgtttc cgtggaaaac ctctttccat agctgtttct gaggatatgg taggtcgggt
     1561 cttcgatgga atggggaatc cgattgatgg cggtccggaa atcattccgg aaatgagcct
     1621 ggacatcaat ggtcaggcga tcaaccccgt tgcgcgtgat tatccggatg agttcatcca
     1681 aacgggaatt tcggccatcg accatctgaa cacactcgta agaggtcaga aacttcctgt
     1741 gttctccggt tcaggtttgc cacataaaga attggctgct caaatcgctc gtcaagcgac
     1801 ggtgttgaac agcgatgaaa aatttgcggt tgtatttgca gcaatgggga tcacttttga
     1861 agaaaaagaa tatttcatgg aagatttccg caagacagga gcgatcgatc gttccgtcat
     1921 gttcatcaat ttggcgaacg atccagccat cgaacggatt gccacaccta aaatggctct
     1981 gacagtcgcg gaatatctgg cctacgaaaa agacatgcac gttttggtca tcatgactga
     2041 catgaccaac tattgtgagg ctttgcgtga aatttcggca gcacgtcgtg aagtgccggg
     2101 tcgccgtggt tatcccggat acctgtatac gaacctttcc accttgtacg agcgtgcagg
     2161 ccgtatcatc ggcaaaaaag gttcggtgac tcaaatcccg attctttcca tgccggagga
     2221 tgacattacg catccaatcc cggatttgac gggttacatt acggaaggcc aaatcattct
     2281 ttcacgtgaa ttgaacaact caggcatcaa gccgccgatc aacgtgttgc cttcgctttc
     2341 acgtctgaaa gacaaaggta cgggtgaagg caagacaaga aaagaccatg ctccaaccat
     2401 gaaccagttg tttgcggctt acgcaaaagg aaaacaagct aaagaattgg ctgtcatcct
     2461 tggagagtcc gccttgtcca agacggacaa actgtatgtg cagttcacaa agcgtttcga
     2521 agaagaatac atcagtcaag ggttctatac gaaccgcaca attgaagaga ctttggcttt
     2581 gggctgggaa ctgttgtcca tcctgccggt aacggaactt aagagaatca agagcgatat
     2641 gatcgatgag tttatgccga agggagagtg atcgtccatg gcaagattga atgtaaagcc
     2701 cacccggatg gaactgtcca acttgaaatc gcgtctggtg ctatccaccc gcggccataa
     2761 actgctgaag gataagcaag atgaattgat gcgtcaattc atcaatctga ttcgcgaaaa
     2821 caacattctg agggatgaag tcgaaaaaga attgaccgcc tcgatgcgtt ctttcgtggt
     2881 agcgaagtcg cttctgaatg aggctttcat tgaagaactg ttcgctgtac ccgctacggc
     2941 ggttgagttg gacattcaag aaaacaacat catgagcgtc gtcgttcccc aaatgaattt
     3001 ttcaatggtt gatcaagacg ataaatccac agatttacag tacggttacg tcaactcgaa
     3061 cggtgaactg gatgatgcga tcaaaaagat tgaagacatc cttccgaaat tacttaaatt
     3121 aacggaaatc gaaaaaacat gccaacttct tgccgatgag atcgaaaaaa caagaaggcg
     3181 tgtaaatgca ttggaataca aaatgattcc gcaattgcag gagacgatcc gttacatcca
     3241 aatgaagctc gaggagaacg aacggtcgag tatcgtccgc atgatgaaag tcaaagacat
     3301 gggcgtttct tgatgcctcc agaacaagac agggataccc ttgtcttgtt cttttttgac
     3361 atataggaat ttataaaatt ttccagagtg a
//
LOCUS       DAOMUJ010000168         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_7963_length_3388_cov_1.713471, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000168 DAOMUJ010000000
VERSION     DAOMUJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7963_length_3388_cov_1.713471"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..796
                     /locus_tag="PLT73_05200"
     CDS             299..796
                     /locus_tag="PLT73_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM19810.1"
                     /translation="MDTISYYIKEIINATGKGLKGYIISAIKLATISFVLLCLGFVYF
                     GIDFWFLKALGIAVFDLIPILGSGMVMIPWAVIHLLLGNTTLAWQIGLLYIILVVVRQ
                     IAEPFITGKELGIRPLYTFLATVVCILLFGPIGAVLGAVVAVVIKAVLEVSSVSRNNY
                     DKHRR"
     gene            1029..1265
                     /locus_tag="PLT73_05205"
     CDS             1029..1265
                     /locus_tag="PLT73_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRM19811.1"
                     /translation="MTNVRDLPIGVSAIVNKITGQGATRRRLMDMGITKGTAIYVRKV
                     APLGDPIQVMVRGYELSIRKKDAEMIEVTFGEGK"
     gene            1265..>2767
                     /gene="feoB"
                     /locus_tag="PLT73_05210"
     CDS             1265..>2767
                     /gene="feoB"
                     /locus_tag="PLT73_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRM19812.1"
                     /translation="MGKTIALVGNPNSGKTTLFNHLTGSTQRVGNWPGVTVEQKAGTL
                     LHDGKTQVIDLPGIYSLSPYTIEEIVTRDYLTLDAPDLIINIVDASNLERNLYLTTQL
                     MELHLPIVVVLNMMDIVEKRGDQLDVAGLSRAFGLPFVTISALKESGLEELFKAIAEP
                     ISVAEPITYSIVVESILDTIEEILAPLVPKKLRCYYSIKLFERDRQTAGKLAIPEEGQ
                     AVMEGLLSRYEKELDDDSEAILINERYKFVTKITRQVLVKNSEKASFSVVIDHIVTNR
                     WLGLPIFVLIMYAVYYFAITTVGTWGTDWVNDVLFGSIVPEAVQTFFDSIDIHPAVSS
                     LVVDGAIGGVGAVLGFLPQMAALFLCLTLLEDSGYMARVAFVMDRVFRGFGLSGKSFI
                     PLLIGSGCSVPGIQASRTIENLQDRRMTILTTSFIPCGAKLPVIALIANAIFGGASWV
                     ALSMYLLGIATVIFSGVLLRKTAIFSGEASPFVMELPMYHWPQWKSIFRAV"
     assembly_gap    2768..2777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2778..>3388
                     /locus_tag="PLT73_05215"
     CDS             <2778..>3388
                     /locus_tag="PLT73_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM19813.1"
                     /translation="LAFVKNAGTVIFLSSSFIWFLSSFNWQLRMTVESDQSILAKIGS
                     AVAIFFAPLGFGSWQATVATIQGLIAKENVVGTFGVLMNTTGSEAEVAAAFSTLFNPV
                     SAVAFLVFNMICMPCFAAVGAMRTEFGSAKWTLFGVLYQTTLAYVLAFIINQLGSVIV
                     LGAPFGAGASIAAAATAILVFLVVRPAPKKASPMWGSLAKPMV"
BASE COUNT          797 a          801 c          893 g          887 t
ORIGIN      
        1 agcttggctg tctcttcttc attcaggagg ccggccgcgt gtaaggcagt gatcctttcg
       61 gctggggttt tatcgtaaaa tttctgaaga tagttggatt ccattttatg ctccttttcc
      121 caagtgtttt cacgtgtatt cagtgtctgt ttcctataat cttatcatag aaccatcctg
      181 atggcatcaa ccgttcattg agccgaatgc taatgggaaa gaatccgtcc aatgaccgat
      241 gcttggttgg tgcaaagacg tgtatactgg atttatcaag agaaagcgag gggtcgcatt
      301 ggatacgatc agctattata tcaaagaaat catcaacgca accggaaagg gcttgaaggg
      361 atacatcata tcggcgatca agcttgctac aatcagtttt gtcctgttgt gtctgggatt
      421 cgtgtacttc ggcatcgatt tctggttcct gaaggctttg ggaatcgcgg ttttcgatct
      481 catcccgatt ctggggagcg ggatggtaat gatcccttgg gcggtgatcc atctgctgct
      541 cgggaacaca acgttggctt ggcaaatcgg actgctgtac atcattcttg tggttgttcg
      601 tcagattgcg gagccgttca ttaccggcaa ggaactcggc atccggccgc tgtacacgtt
      661 tctagcgaca gtcgtttgta tcctcttgtt cggaccaatt ggagccgttc tcggtgcagt
      721 ggttgctgtc gtgatcaaag ccgttttgga agtctcgagt gtcagccgaa acaattatga
      781 caaacatcgt cgctgaaaag agacaatatt tcacaagcac gggagagatt gacttcttcc
      841 gtgccttttt attttttcaa acccttatga gcctgatgca tccacgcatt actgatgaaa
      901 aaaaataagt aaaaatgcac gaattatctt gacccgaagg agtctatgcc ttaagctatg
      961 acttaatgag aacggttctc aattagtgtc gggaaattat tattacttaa agaatggagg
     1021 ggacgatttt gacgaatgta agggacttgc cgatcggcgt aagcgcgatt gtgaacaaga
     1081 taacaggaca aggcgctacc cgcagaagat tgatggatat gggcatcacg aaaggcactg
     1141 ccatttatgt cagaaaagtc gctccattgg gcgatccgat ccaagtgatg gtgcgcggat
     1201 atgaattatc gatacgcaaa aaagatgccg agatgatcga agttacgttc ggggagggaa
     1261 aatgatgggg aaaaccatag cgctggtcgg aaatcccaac agcgggaaaa cgactctatt
     1321 caaccatttg acaggttcca cccaacgcgt cggcaactgg ccaggggtta ccgtcgaaca
     1381 gaaggccggc acgctactgc atgacggcaa aacgcaggtc atcgatctgc cgggcatcta
     1441 ttcgctttcc ccttacacaa tcgaggaaat cgtcacgcgc gactatttga cgctggatgc
     1501 accggatctg atcatcaata tcgtcgatgc ctccaatctg gagcgaaatc tttacctgac
     1561 tacccaattg atggaattgc atctgccgat cgttgtcgtt ctcaacatga tggacatcgt
     1621 cgagaaacgg ggcgatcagc tggacgtcgc cggtttgtcg cgcgcattcg gattgccgtt
     1681 cgtaacgatt tcggcactga aggaaagcgg attggaagag ttgttcaagg ccatcgctga
     1741 acctatatca gtagcagaac cgattacata cagtatcgta gtggaaagca tccttgatac
     1801 gatcgaagaa attcttgcgc cattggttcc gaaaaaatta cgttgctact acagcatcaa
     1861 gcttttcgaa agggacagac aaactgccgg aaaacttgcg attcccgaag aaggccaagc
     1921 tgtcatggaa ggtctgctca gccgttatga aaaggaattg gacgacgact ccgaagcaat
     1981 cctgattaac gaacgctata aatttgtgac gaaaattacg cgccaagtgc tcgtgaaaaa
     2041 tagcgaaaaa gcctccttct cagtcgtgat cgatcatatc gtaacgaacc gctggcttgg
     2101 gttgccgatt ttcgtcctga tcatgtatgc ggtctactac ttcgccatca caactgtggg
     2161 gacgtggggg actgactggg taaacgatgt gttgttcgga agcatcgttc ctgaggctgt
     2221 gcaaacgttc ttcgacagca tcgacatcca tccagcagtc agcagtctcg tggtcgatgg
     2281 tgccatcgga ggagtaggcg cagttcttgg cttcctgccg cagatggccg ccttgttcct
     2341 ctgcttgaca ttgctggagg acagtggcta tatggcccgc gtcgctttcg tgatggatag
     2401 ggtattccgc ggcttcggtc tgtcgggtaa atcgttcatt cccttgctga tcggttccgg
     2461 ttgctcggtt ccgggcatcc aggcttcccg cacaatcgag aacctgcagg atcgccgcat
     2521 gacgatcctg accacttctt tcattccctg cggcgcgaaa cttccggtca tcgccctgat
     2581 cgccaacgcc atcttcggcg gcgcatcctg ggttgccttg tcgatgtact tgttggggat
     2641 tgcgactgtc atcttttccg gtgtattgct gcgtaagaca gcgatctttt ccggggaagc
     2701 atcgccattc gtcatggagt tgccgatgta tcactggccg caatggaaaa gcattttccg
     2761 cgcggtannn nnnnnnngcc tggccttcgt caaaaatgca ggaactgtca tcttcctcag
     2821 ctctagcttc atttggtttt tatcttcctt caactggcag cttcgaatga cggttgaaag
     2881 tgaccaaagc atcctggcca aaatcggcag tgcagtagct atattcttcg cgccgttggg
     2941 cttcggctct tggcaagcga cggtggcgac catccaagga ttgatcgcga aggaaaatgt
     3001 tgtcggcaca tttggtgtat tgatgaacac aaccggttcg gaggcggagg tagctgccgc
     3061 attcagcaca ctgttcaacc cggtgagcgc agtagcgttc cttgtgttca atatgatctg
     3121 catgccttgt ttcgcggctg tcggagcgat gcgcaccgaa ttcggcagcg ctaaatggac
     3181 gcttttcggc gtcttgtatc aaactacttt ggcttatgtc ttggccttca tcatcaatca
     3241 gctcggatcc gtcatcgtgt tgggtgcacc ttttggggcg ggagccagca tagcggctgc
     3301 agcaacggca atattggtgt tcttggtcgt cagacctgca ccgaaaaaag cttcgccgat
     3361 gtggggcagc ttggcgaaac cgatggtc
//
LOCUS       DAOMUJ010000169         3345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8081_length_3345_cov_2.028267, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000169 DAOMUJ010000000
VERSION     DAOMUJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_3345_cov_2.028267"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1925
                     /gene="glgB"
                     /locus_tag="PLT73_05220"
     CDS             15..1925
                     /gene="glgB"
                     /locus_tag="PLT73_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRM19814.1"
                     /translation="MSAKMNILKELEKEMYLFNIGEHLESYRFLGSKQRTENGIEGWR
                     FTVWAPHAKTVSLVGDFTEWKPVEMQKIGKTGGWSHFTVAAAQGDCYKYYIEDKNGKK
                     KYKIDPYSFAYEVPPKDASVVFDMPEKKWKDGRWAANKKRKSIYQKPLNIYEVHFSSW
                     KKHDDGSWYSFKDLTETLIPYVKEMGYTHIEFMPLMEHPLEASWGYQITGYFAVAARY
                     GNLLELRDFVEEAHKEGIGVIMDWVPGHFCKNDYALSYFDGTPTFEYADPNRAINNRW
                     GTLNFDLGKAQVHSFLISNAIFWLQEFHIDGIRVDAVSNMLYLDYDEGPWTPNEDGSN
                     HNRQGIEFLKKMNTKVFERQPDTYMIAEESTAWANVTKPIEAGGLGFNYKWNMGWMND
                     TLRFFEMDPLFRKDNFNLITFSFMYAFNENFILPISHDEVVHGKQSLLAKMPGDRYKQ
                     FAGLRTLQAYMMAHPGKKLNFMGNEIGQFLEWRFYDQIEWGVLNNEFNPEFQHYIKTL
                     NHLYKDTKALHEVDDANTGLELLDADNNQESVLSFIRTGDKPRDFLIVVCNFTPVERR
                     KFQVGAPYEGHYEVLLNTEMQEFGGTWTKNIPEMITSLDGMNRQPFSIEFTLPALGVL
                     FIKPKRVFGVNK"
     gene            1984..3132
                     /locus_tag="PLT73_05225"
     CDS             1984..3132
                     /locus_tag="PLT73_05225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010012705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRM19815.1"
                     /translation="MKNQMVAMILAGGQGTRLGKLTRETAKPAVPFGGKYRIIDFALS
                     NCANSGINKVGVVTQYQPLELNEHIGNGESWGLNGGNGGATILQPYSSADGEKWFKGT
                     AHAIYQNIAYIDRYNPEYVLVLSGDHIYKMDYSHMLDFHKANHASLTVGVIPVPMEEA
                     PRFGIMNTDQTNRIIEFDEKPKEPKSNLASMGIYIFNWQVLRKYLVEDQAKQREMEDF
                     GKNVIPAYLENGENCFAYAFDGYWKDVGTIESLWEANMEFLDPNHILNIRDEEWRIYS
                     KNPVSPPQFLTETSNVTDSMIVDGCYVAGEITHSILSQNVRVGNGSVVRDSLIMANVT
                     IGENVTIEHAIIGENAKIADNANVIGKNGEIEVVGYAEVIGGLKDEDE"
     gene            3119..>3345
                     /locus_tag="PLT73_05230"
     CDS             3119..>3345
                     /locus_tag="PLT73_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HRM19816.1"
                     /translation="MKMNSICAILNLTEDEKALKPLTQARPIASLPFASRYRLIDFNL
                     SSISHAEINSVGIFIAGSGRSIYDHIRSGSV"
BASE COUNT         1001 a          689 c          824 g          831 t
ORIGIN      
        1 agggaagtgt ctggatgagt gcaaaaatga acatacttaa agagttggaa aaagagatgt
       61 atttgttcaa catcggggaa catcttgaaa gttaccgctt tttaggaagt aaacaacgga
      121 ccgaaaatgg gattgaagga tggcgtttca cagtctgggc accccatgca aaaactgttt
      181 ccttggtcgg ggattttact gaatggaaac ccgtagaaat gcaaaaaatc ggcaagaccg
      241 gagggtggtc gcattttacg gtggctgctg cgcaaggaga ctgttacaaa tattatatcg
      301 aagacaaaaa cggcaagaaa aaatataaaa tcgatcctta ttctttcgcg tatgaagttc
      361 ctccaaaaga cgcgtcagtt gtgtttgaca tgcctgagaa aaaatggaaa gacggcagat
      421 gggcggcaaa caaaaaaaga aagtccattt atcaaaaacc gcttaacatt tatgaagtgc
      481 atttcagttc atggaaaaag catgatgatg gcagctggta ttcctttaaa gatcttactg
      541 aaacgttgat tccatatgtg aaggaaatgg gctacacgca catcgaattc atgcctttga
      601 tggaacatcc gttggaagct tcttggggct atcaaatcac cgggtacttc gcggtggctg
      661 cgcgttacgg gaatttgctt gagctgaggg attttgtgga agaagcgcat aaagaaggaa
      721 tcggcgtcat catggattgg gttcccggcc atttctgcaa aaatgattat gcgttgtcct
      781 attttgatgg tacaccgact tttgaatatg ccgatccgaa tcgcgccatc aacaaccgtt
      841 gggggacatt gaattttgac ttgggaaaag cacaagtgca cagcttcttg atttcgaacg
      901 ccattttctg gctgcaagag ttccatattg atggaatccg tgtcgatgcc gtttcgaata
      961 tgctttatct tgactatgat gaaggtccat ggacaccgaa tgaagatggc agcaatcaca
     1021 atcgccaagg aatcgaattc ctcaagaaga tgaacacaaa agtattcgaa cgccagccgg
     1081 acacatacat gatcgcggaa gagagtactg cttgggccaa cgtcacgaaa ccaatcgagg
     1141 caggtgggct tgggttcaac tataaatgga atatggggtg gatgaatgat accttgcgct
     1201 tcttcgaaat ggatcctttg ttccgaaaag acaatttcaa cctgatcacc ttttcgttta
     1261 tgtatgcttt caatgagaac tttatactgc ctatttctca tgatgaagtg gtccacggga
     1321 aacaatccct tttggccaaa atgccgggag accgctataa gcaatttgcg ggcttacgga
     1381 cgctgcaagc ctatatgatg gctcacccag gcaaaaaatt gaacttcatg gggaatgaaa
     1441 tcggtcaatt cctggaatgg cgcttctatg atcaaatcga atggggtgtc ctgaacaacg
     1501 agttcaatcc ggagttccag cattacatca aaaccttgaa ccatctttac aaagatacga
     1561 aagctttgca tgaagtcgat gatgcaaata cgggtctgga gcttcttgac gcggacaata
     1621 accaggagtc agtcctttct ttcataagaa ctggtgataa accgcgcgat tttctgattg
     1681 tcgtgtgcaa cttcacgcct gtcgagcgac gtaaattcca ggtaggcgca ccgtacgaag
     1741 gccactacga agtattattg aatactgaaa tgcaggaatt cggcggtaca tggacaaaaa
     1801 atattccgga aatgatcacc agcctggacg gcatgaaccg tcagcctttc tcgatcgaat
     1861 tcacattacc cgcattagga gttttgttca tcaaaccaaa acgcgttttt ggtgttaata
     1921 aatagataaa cagatagata cgatgagaag gaggggaaag gaagaggcat tcttcctgat
     1981 actatgaaaa atcaaatggt agcaatgata ttagccggag ggcaaggaac tcggctagga
     2041 aaattgacgc gcgaaacagc caaaccggcc gttccgttcg gaggaaaata ccgaattatc
     2101 gattttgctt taagtaactg cgcgaactca ggcatcaata aagtcggggt agtgacacag
     2161 tatcaacctt tggaattgaa tgagcacatc ggcaacggtg aatcatgggg actgaatggc
     2221 ggtaatgggg gtgctacgat tctgcagccg tattcgagtg ctgatggcga aaagtggttc
     2281 aagggaactg cgcatgccat ttatcaaaat atcgcctata tcgacagata caatccggaa
     2341 tatgtcctgg tattgtccgg tgaccatatc tacaagatgg actactcgca tatgctggat
     2401 ttccataaag cgaaccatgc cagcttgact gtcggcgtca tccctgttcc gatggaagaa
     2461 gcaccccgct tcgggatcat gaatacggat cagacgaacc gcatcatcga attcgatgag
     2521 aagccaaaag agccgaaaag caatttggca tcgatgggta tctacatctt caactggcaa
     2581 gtgctccgta aatatttggt ggaggatcaa gcgaaacagc gtgagatgga agatttcggc
     2641 aagaacgtca ttccggccta ccttgagaac ggcgaaaact gctttgcgta cgctttcgac
     2701 ggctactgga aagacgtcgg tacaatcgaa agcctttggg aagcaaacat ggaatttttg
     2761 gatcctaacc atatcctgaa catcagggat gaagagtggc gcatctattc aaaaaatccg
     2821 gtatcccctc cacaattctt gacggagaca tccaatgtga cggattccat gatagtggat
     2881 ggttgttatg tagcaggtga aatcacccat tcgatccttt cccaaaatgt tcgggtcggc
     2941 aacggatctg tcgtgcgtga tagtttgatc atggccaatg taacgattgg ggagaatgtc
     3001 acaatcgagc atgccatcat cggtgaaaat gcaaaaattg ctgacaatgc caatgtcatc
     3061 ggcaaaaacg gtgaaatcga agttgtcgga tatgctgaag ttataggagg actgaaagat
     3121 gaagatgaat agtatctgtg ccatattaaa cctgaccgag gacgaaaaag cgttgaaacc
     3181 tttgacgcag gcccgcccga ttgcttcact accgttcgct agccgttacc gattgatcga
     3241 cttcaatctt tcaagcatca gccatgctga aatcaactca gtgggtattt tcatcgcggg
     3301 cagtggccgt tcaatctatg accatatccg cagtggatct gtatg
//
LOCUS       DAOMUJ010000170         3323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8162_length_3323_cov_2.758262, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000170 DAOMUJ010000000
VERSION     DAOMUJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_3323_cov_2.758262"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1278)
                     /locus_tag="PLT73_05235"
     CDS             complement(94..1278)
                     /locus_tag="PLT73_05235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRM19817.1"
                     /translation="MKKITITQISEKKIKAGNPLLQMEDFPNELSFDEGELVELVDSR
                     QKFVAKAYLAKQNKGVGWVFSLDNADSFNEAFFKKQFEKAKRKRTDLQSDPETTTYRL
                     FNAEGDGIPGVTIDHYDGFAVVSWYSEGIFRYQTAIIAAFQAVFPETKGIYEKFRYQR
                     KDGLISRYVRGDAAPTPLIVKENGINYATYLDDGLMTGIFLDQREVRGALTERYAAGK
                     RVLNTFSYTGAFSVAAAMGGAIETTSVDVANRSLERTKEQFAVNNLDGEQQKIYVMDV
                     FDFIRYAIRKELQYDVTIIDPPSFARTKKRTFSVTKDYTQLLEELIQITAADGTLIVS
                     SNAANYKEKNFKQDIAQAFKNSRCDYRIMETFHLPNDFAVPPGSETSDYLKVFIIKKL
                     AR"
     gene            complement(1301..1924)
                     /locus_tag="PLT73_05240"
     CDS             complement(1301..1924)
                     /locus_tag="PLT73_05240"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM19818.1"
                     /translation="MIFLQGLYEWVAASLESSVNHFPLIQLVFNSVDKTILYYLIFLV
                     LRAAYLLRRHRKTGKKIPLYKEGLLHALFIYIVLLLQLTVFRNEVTFWTVEYHKIDWS
                     AIHWLPLVDTVKLFYGDSGFSAWYNSWGNVVWFMPLGYMGPYLFRKKCSFSKVTAMGF
                     LFSSMIEFLQLIFQTGVTHIDDVFFNTLGAAIGYSIWVSSKKAVQNG"
     gene            2187..>3323
                     /locus_tag="PLT73_05245"
     CDS             2187..>3323
                     /locus_tag="PLT73_05245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM19819.1"
                     /translation="MKQKLTEQLQTRFGFFLFAVVLFWLKTYFAYHTAFSLGVDGWFQ
                     QLILFINPIATTLLILGISLFVKSPKKGYRSLIALYVLNSILLFANIIYYREFTDFLT
                     IKTIFGSANATKNLGSGVIAMMQPLDLFYWIDAFVLIYVYKKFVSKNRDERIFKKRWA
                     FATVAASIALFAANLGLAEISRPQLLTRTFDRNYIVKYLGINVFTLYDGIKTAQANQV
                     RASADSSDMAAVLDYLGTHYAEPNEANFGQAEGKNVVYIHLESMQQFLIDLSLTDETG
                     MTAEVTPFLNSLYHSSDSYSYSNIFHQTGQGKTSDAELMLDNSLFGLPQGSAFTQIGA
                     DNTFQSAPAILGETFGYTSAVFHGNVGSFWDRDNVYKSFDYDYFF"
BASE COUNT          863 a          822 c          715 g          923 t
ORIGIN      
        1 aggaagtctc tcagcaattg cgctcttcga tgctcattca gggtcagatt tcctaaattt
       61 ctttcggggc tgagcgaacc cgttcagctt ttcttatctc gccagtttct taatgatgaa
      121 gactttcagg taatcgcttg tttcgctgcc tggcggaaca gcaaaatcgt tcggcagatg
      181 gaatgtctcc atgatgcggt aatcgcatcg gctgtttttg aaggcttggg cgatatcctg
      241 tttgaagttc ttttccttgt agttggcagc gttggacgac acaatcagcg tgccatccgc
      301 tgcggtgatc tggatcaatt cctccaacaa ttgcgtataa tctttcgtta ccgaaaatgt
      361 gcgctttttt gtgcgggcaa agcttggcgg atcgatgatg gtcacgtcat actgcaattc
      421 ctttcggatc gcgtagcgga tgaaatcgaa gacatccatg acatagattt tctgttgctc
      481 gccatccagg ttattgacgg cgaattgttc ctttgtccgt tccaacgaac ggttcgcaac
      541 atcgacactc gtcgtttcga tcgccccgcc catggctgct gccacggaga acgcgccggt
      601 gtagctgaac gtgttcaaga cccgtttgcc tgccgcataa cgctccgtca gagcgccgcg
      661 tacctcgcgc tgatccagaa aaatgcccgt catcagtcca tcgtccagat acgtcgcata
      721 gttgatgccg ttctccttca cgatcagggg cgtcggggct gcatcacccc tgacgtagcg
      781 gctgatcagg ccatccttcc gttggtaacg gaatttttca tagatgccct tcgtttccgg
      841 gaagaccgct tggaaggcag cgataatggc ggtctggtag cggaagatgc cttcgctgta
      901 ccaagatacc accgcaaacc cgtcgtaatg atcgatggta acaccaggga taccgtcccc
      961 ctccgcattg aaaagccgat aggtagtcgt ttccgggtcc gattgcaagt ccgtgcgctt
     1021 acgtttcgct ttttcaaact gttttttgaa gaatgcttca ttgaaactat ccgcgttatc
     1081 caaggaaaat acccaaccga cgcctttgtt ctgtttagcc aaataggctt ttgcgacgaa
     1141 tttttggcgg gaatcgacca gctcgaccag ttccccttca tcgaagctga gctcgtttgg
     1201 gaaatcttcc atttggagca agggattgcc tgccttaatt tttttctcgc ttatttgtgt
     1261 aatcgtaatt tttttcataa tttacttcct ttatagtcga ttatccgttc tgcactgctt
     1321 tttttgatga tacccatatg ctgtagccga ttgcggcacc caacgtatta aagaagacat
     1381 catcgatatg cgtgacgccc gtctgaaaga tgagctggag gaactcaatc atgctggaaa
     1441 aaagaaaacc catcgctgtc actttggaga aactgcattt ttttctgaac agataaggcc
     1501 ccatgtagcc cagcggcata aaccagacaa cgttccccca tgaattgtac catgccgaaa
     1561 atccggaatc cccataaaag agtttgaccg tatccaccaa cggcagccaa tggatcgctg
     1621 accaatcgat tttgtgatac tcgacggtcc agaaggtcac ctcattgcgg aaaaccgtca
     1681 gttgaagcaa caatacgatg tagataaaca aagcatgcag cagcccttcc ttatataagg
     1741 ggattttctt gcccgtcttg cggtgtctgc gcagcaggta ggcggctctt agaaccagaa
     1801 aaatcaaata gtacaatatg gtcttgtcca cgctgttgaa aactaattgt atgagcggaa
     1861 aatgattaac ggatgactcc agcgaagcag ccacccactc atacagccct tgtaaaaata
     1921 tcataatcta cgctcacctt tgtcagtttc gttcctttaa acagatactt tgaaagtccc
     1981 attttctcga aaaatcctta aaaaagcaac tattcttgag aaaaacttag gaaaaacttt
     2041 acggggcttc gattgactat caaaagcatt cagaatataa tgacactagc tcattttaca
     2101 taaaattcac aaacaacaca cataaaccaa aaaaattcac aggaaattta catgtgttcc
     2161 acacgatatc ataggaggtc tcttttttga aacaaaaatt aactgaacag ttgcagacgc
     2221 gattcggatt tttccttttc gccgtcgtcc ttttctggct gaagacctat ttcgcttatc
     2281 acacagcgtt ctctttaggc gtagacggtt ggtttcagca gctgattctt tttatcaacc
     2341 caattgccac gactttattg attctcggga tttcgctttt tgttaagagc cctaaaaaag
     2401 gctacagaag cttgattgct ctgtatgtac tgaattcgat cctgttgttc gccaacatca
     2461 tctactaccg cgaatttacg gacttcctga caatcaaaac catcttcggt tcagccaacg
     2521 caaccaaaaa cttaggttcc ggagtcatcg ccatgatgca gccgctggat ctgttctatt
     2581 ggatcgatgc ttttgtcctc atctatgtct ataagaaatt cgttagcaaa aatcgcgacg
     2641 aacgtatctt caagaaaaga tgggcgtttg cgacagttgc cgcctcgatc gctctcttcg
     2701 ccgctaactt aggccttgct gaaataagcc gaccgcaatt gctgacaaga acttttgatc
     2761 gcaactacat cgtcaaatac ttgggtatca atgtcttcac cctctatgat ggcatcaaga
     2821 ctgctcaagc caaccaagtg cgcgcaagtg cggacagctc agatatggct gccgttcttg
     2881 attacttagg tacacattat gccgaaccca acgaagcaaa cttcgggcaa gcagaaggca
     2941 aaaacgtcgt ctacatccat ctggaaagta tgcagcagtt cctgatcgat ctgtcattga
     3001 ctgatgaaac aggcatgacg gctgaagtga caccattcct gaacagcttg tatcacagca
     3061 gtgattctta cagttacagc aacattttcc atcagacggg ccaaggaaaa acaagtgatg
     3121 ccgaactgat gctggacaat tcactgttcg gattgccgca aggttcggcc tttacacaaa
     3181 tcggtgcgga caacaccttc caatcagcac cggccatctt gggcgaaacc tttggctaca
     3241 catcagctgt tttccacggg aatgtcggtt ctttctggga tcgcgataat gtttataaat
     3301 ctttcgacta tgattatttc ttt
//
LOCUS       DAOMUJ010000171         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8213_length_3308_cov_2.080234, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000171 DAOMUJ010000000
VERSION     DAOMUJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_3308_cov_2.080234"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2164
                     /locus_tag="PLT73_05250"
     CDS             <1..2164
                     /locus_tag="PLT73_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM19820.1"
                     /translation="QNQEDQYEALEKYGIDLVQAVRSGKQDPVIGRDEEIRDVIRILS
                     RKTKNNPILIGEPGVGKTAIVEGLAQRIVKKDVPDNLKDKTIFSLDMGSLIAGAKYRG
                     EFEERLKAVLKEVKKSDGRIILFIDEIHTIVGAGKTEGSMDAGNLLKPMLARGELHCI
                     GATTLDEYRQNLEKDKALERRFQKVLVKEPTVSDTISILRGLKERYEIHHSVNIHDNA
                     LVAAATLSDRYITDRFLPDKAIDLVDEACANIKVEMNSMPTELDQVSRKLMQLEIEEA
                     ALKKESDDQSKKRLAALQVELADLREESNELRMKWGNEKEEVEKLRAKREDLEDARRQ
                     LEDAEANYDLERAAVLRHGSIPQMEKELAALEKENATRRNKAGNLVQESVTENEIATV
                     VGRMTGIPVTKLVEGERDKLLKLEETLHKRVIGQEEAVESVTNAVLRSRAGLQSPNRP
                     IGSFLFLGPTGVGKTELAKALAENLFDSDEHMVRIDMSEYMEKFSVSRLIGAPPGYVG
                     YEEGGQLTEAVRRSPYTIILLDEIEKAHPDVFNILLQVLDDGRLTDSKGRLIDFKNTV
                     LIMTSNIGSQLLLEGVHPDGTIPDAVANEVMKVLKSSFKPEFLNRIDDTILFTPLSKE
                     DVKRIIVKMVKELSSRLVDKQIQLSVSDEVTSWIAENAYDPIYGARPLRRFISAEIEN
                     PLARAIIKGEIHENQSVTVTLVGNQVGFQTVDFDEFQQ"
     gene            2318..2920
                     /locus_tag="PLT73_05255"
     CDS             2318..2920
                     /locus_tag="PLT73_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRM19821.1"
                     /translation="MLFLVIGFLCLVFMLFTSKYYLFGAAAILSFLLYFLYFGSGNWL
                     TLLLFLFGIMLLVVELFIPDFGLVGVAGFLMVVAGLFLNNERILESVLDVSLAIIISG
                     VATSVLLKKGYKFLPDRSKLILDTSLNRERGYSSSKDYSEFLGMIGTATTTLRPAGKV
                     AIGGVVLDVVSDGKIIFEGKQIRVIQVEGVKITVKELDEE"
     gene            2917..>3308
                     /locus_tag="PLT73_05260"
     CDS             2917..>3308
                     /locus_tag="PLT73_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HRM19822.1"
                     /translation="MTEGLIGLIFIALFVVLVLSLFFRFVPVGLWITAYFSGVKVKIS
                     NLVGMRLRRVVPSMIVQPMIKATKAGLIIDINELEAHHLAGGDVNMVIDALIAAQRAD
                     IDLGFEKAAAIDLAGRNVLEAVKMSVNP"
BASE COUNT          900 a          638 c          928 g          842 t
ORIGIN      
        1 gcagaatcag gaagatcagt atgaagcatt ggaaaagtat ggcatagacc tcgtgcaagc
       61 ggtgcgtagc ggaaaacaag atccggtaat cggcagggac gaagaaatta gggatgtcat
      121 ccgcattttg tcgcggaaaa caaaaaataa tccgatcctg ataggtgaac ctggcgtagg
      181 caagaccgcc attgtggaag gtttggctca acggattgtg aaaaaagatg taccggataa
      241 tttgaaagac aaaacaattt tttcgttgga tatgggctca ttgattgcgg gagctaagta
      301 ccgtggggaa ttcgaggaac gccttaaagc ggtattgaag gaagtgaaga aaagcgacgg
      361 ccgcatcata cttttcattg atgagatcca tacgatcgtc ggggcaggaa aaaccgaggg
      421 gagtatggat gccggcaatc tgctgaaacc tatgctggcc agaggcgaat tgcactgcat
      481 cggagcgacc actttggatg aataccgtca aaatctggag aaggataagg ccttggagcg
      541 aagattccaa aaggtgttgg tgaaagaacc gacagtttct gatacaatca gcatcttgag
      601 ggggttgaag gaacggtatg agatccacca cagcgtcaac atccatgaca acgcccttgt
      661 tgcggccgcg accttatccg atcgttacat cactgaccgg tttctgccgg ataaagcgat
      721 cgatcttgtc gatgaggctt gcgcgaacat aaaagtggaa atgaattcga tgcctaccga
      781 gttggatcag gtgagccgca aattgatgca actggagata gaagaggcgg ccttgaaaaa
      841 ggaatccgat gatcagagca agaaaagatt ggcggctctg caagtcgagt tggctgatct
      901 gcgcgaagaa tcaaatgagt tgcgcatgaa atggggtaat gaaaaagaag aagtcgaaaa
      961 gctgcgtgcc aaacgggagg acctggagga cgcgcgcaga caattggagg atgccgaggc
     1021 caattatgat ttggaacggg cggcggtttt gcgccacggc agtatccctc agatggaaaa
     1081 ggaattggcg gctcttgaaa aagaaaatgc gacccgaagg aataaagcag gcaatctagt
     1141 ccaggaatcg gtcaccgaga acgaaatcgc gacagtggtg gggcggatga ccggcatccc
     1201 tgtgacgaag ctggttgaag gtgaaaggga taaactcctg aaattggagg aaacgctgca
     1261 caagcgtgtc attggccaag aggaagcagt agaaagtgtc acgaatgccg ttttgcgttc
     1321 gcgtgctggc ctccaatcgc cgaaccgtcc gattgggtcc ttccttttct tgggaccgac
     1381 aggagtcggg aaaacggagt tggccaaagc cttggcggaa aatttgttcg actccgatga
     1441 acatatggtt cggatcgata tgagcgaata catggaaaaa ttttcggttt ctcggctcat
     1501 cggggcaccc cctggctatg tcggctacga agagggcggc caattgacgg aagcggtccg
     1561 ccgaagcccc tatacgatca ttctattgga tgaaatcgag aaggcgcacc cggatgtctt
     1621 caatatttta ctccaagtat tggatgatgg acgtttgacc gattcgaagg ggcgtctgat
     1681 tgatttcaaa aacaccgtcc tgatcatgac cagcaacatc ggatcacaac tcctgctgga
     1741 aggcgtccat cccgacggca ccattccgga tgctgttgcg aatgaggtca tgaaagtatt
     1801 gaagagttcg ttcaagccgg aatttttgaa caggatcgat gacaccattc tgtttacgcc
     1861 tttaagcaaa gaagatgtaa aaaggatcat cgtgaaaatg gttaaggagc tatccagccg
     1921 attggtggat aaacagatcc agctttccgt ttctgatgag gtgacgagtt ggatagcgga
     1981 aaatgcttac gatccgattt atggcgcgcg gccgctcaga agattcattt cagccgaaat
     2041 cgaaaatccc ttggcgcggg ccatcataaa aggggaaatt catgaaaatc agtctgtgac
     2101 ggtgactttg gtagggaatc aggtcggatt ccagaccgtc gatttcgatg aatttcaaca
     2161 ataatttgac atgtctacaa acagagagga agtcctgaaa gggatttcct tttgttattt
     2221 ctgtatttgc ggtaaaatga tagtataaat tttcttattt ttatgcgtac gaccgtttgt
     2281 tgtttctttc ggaacaaagc agaaggaggc gttcatattg ctgtttttgg tcatcggatt
     2341 tttgtgtctg gtttttatgc tgttcacttc gaagtattat ctcttcggag ctgcggcgat
     2401 cctgagtttt ttgttgtatt tcctgtattt tggtagtggg aactggctga cgttgctact
     2461 gttcctgttt ggaatcatgt tattggttgt tgaattgttc atacctgatt tcggtcttgt
     2521 aggagtcgcg ggtttcttga tggtcgtagc cggcctgttt ttgaacaatg aacgcatttt
     2581 ggaaagtgta ctggacgtaa gcttggcaat catcatttcc ggtgtggcca cgtcagtgct
     2641 tttgaaaaaa ggttataaat tcttgccgga tcgcagtaaa ttaattctgg acacgtctct
     2701 gaaccgggag cgcgggtatt cgtccagcaa agactattcc gaattcttgg gaatgatcgg
     2761 aacagccacg acgacgctgc ggccagccgg aaaagttgca atcggaggtg tggtgctcga
     2821 cgtagtcagt gacggaaaga tcatatttga aggaaaacag attcgggtta tccaggttga
     2881 aggggtcaaa attacagtta aggagttgga tgaagaatga cagaaggctt gataggactc
     2941 atctttattg cattgtttgt cgtactggtt ttatctttgt ttttccgctt tgtgccggtc
     3001 ggtctatgga tcacagccta cttttcggga gtgaaagtca aaatttcaaa tttggtcggg
     3061 atgcgcttgc ggcgagtggt tccttccatg atcgtacaac cgatgattaa agcgacaaaa
     3121 gcggggctga tcattgatat aaatgaattg gaggcgcatc atcttgctgg tggagatgtc
     3181 aatatggtca tcgatgcgct gattgcggcg caacgtgccg atattgattt aggttttgaa
     3241 aaagcggcgg ccatcgattt ggctggtcgt aatgtgttag aagcagtgaa aatgagcgtc
     3301 aatccaaa
//
LOCUS       DAOMUJ010000172         3300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8240_length_3300_cov_1.837596, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000172 DAOMUJ010000000
VERSION     DAOMUJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8240_length_3300_cov_1.837596"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLT73_05265"
     CDS             <1..233
                     /locus_tag="PLT73_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HRM19823.1"
                     /translation="FDDSATEPLQQVSVIHRFIEPQEWFELADGDRILFDEQEYRISY
                     IGNHVLRNLQSIGHTTMIFKNWDEERLEQAFI"
     gene            307..1503
                     /locus_tag="PLT73_05270"
     CDS             307..1503
                     /locus_tag="PLT73_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM19824.1"
                     /translation="MERAPRKGTSRNRLSWFEKWFLNNKFVTVLLITLLILLIVLVFS
                     KISYLLGPIGSFFSVIGFPLVMAGIFFYMLNPFVTLLEKRGIKRFVGIWIAFVLVLLM
                     MIWGFAILIPIIRDQTIGIVREFPTYWQAIESMTIELINYDWFTSLQEQISEINADIF
                     NTVSEKLNEVLSNTVSGLGSVVGLLTNAFVGIVTMPIILYYLLKEGDKLPLTFLQIFP
                     TNLRESIGDLLKKVNTQISQYVRGQITVAFFVGLMFVIGYAIIGMKFGIVLGIFAGFL
                     NIIPYMGSFIAMVPAVIVAIVDSPLMLAKVLLVFSIEQFIEGRVISPQILGSNLEVHP
                     VTIIFVLLTAGKLFGLTGFILGIPGYAVLKVLFMHIFEWYKEISGLYLDEPEIEEEPE
                     EDYLQE"
     gene            1528..>1778
                     /locus_tag="PLT73_05275"
     CDS             1528..>1778
                     /locus_tag="PLT73_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRM19825.1"
                     /translation="MNKKRWIAVAIALILFVASLGSQFLSTRLADQTEDSLSQLTGNL
                     IPGTSLQENVMESGDASNRIAVLHIEGTIQSGAGSVLTDG"
     assembly_gap    1779..1788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1789..2577
                     /gene="sppA"
                     /locus_tag="PLT73_05280"
     CDS             <1789..2577
                     /gene="sppA"
                     /locus_tag="PLT73_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRM19826.1"
                     /translation="SSYDHDLFLEQLKAIEEDDTIKGIFLIVDSPGGGVYESAEAHDK
                     LYRIAAEKELPVYASMQGTAASGGYYISAGADKIYATAETTTGSIGVIMQAIEYSGLL
                     EKLGIEYNTIKSGSLKDIGSPDRDMTEEERALLRAYVDEAYQRFVTVVSTGRDMPEEE
                     VRAVANGMIYSGTQAQALGLVDEIAYTDDALKAMKTAYGLEDAEVFDYTSTPGLFSDF
                     SWLFAQAAAKLTSQENQTLSELQLLRDTFGTTAAPRLLYLYGGE"
     gene            2577..3143
                     /locus_tag="PLT73_05285"
     CDS             2577..3143
                     /locus_tag="PLT73_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRM19827.1"
                     /translation="MMEEQTTTHEERLEEIRKKKIAWEEAERYSDQQERPFHRYPDYV
                     FAGFWIRLFAYLVDLLIVQALVGILIKPIFALGGISMSESSPLTLYGFIQLLLFVGYF
                     MLTTKYTNGQTVGKMIFGIRVVCFKEENLSWQTVLIREGVGRYIGKTVAVIYLVAAFQ
                     SKKQHPIDLLCDTSVVTENSVRALQESY"
BASE COUNT          825 a          710 c          871 g          884 t
ORIGIN      
        1 tgttcgatga cagcgcaacg gagcccttgc agcaagtttc cgtcatccat cgtttcatcg
       61 aaccccagga atggttcgaa ctggcggacg gagaccggat tttgtttgat gaacaggaat
      121 accgcatatc ctatatcggt aaccatgtcc tcagaaattt acagtcaatc ggtcacacca
      181 ccatgatttt caaaaattgg gatgaagaac gcctggaaca agcatttatt taacccctca
      241 cattttaccg acaataacgg atggcacgat catttcctat aggaagaaag attgaaggtg
      301 agtccaatgg aaagagcccc acgtaaagga acgtctagga atcgtttgtc gtggttcgaa
      361 aaatggtttc tgaacaacaa gttcgtgact gttctattga ttactctgct cattcttctg
      421 attgttttgg tattttcaaa aatctcttat cttttagggc caatcggcag cttcttcagt
      481 gtcatcggct ttccgctggt aatggcgggt attttctttt acatgctgaa cccttttgta
      541 acgctgttgg agaagagagg gataaaacga ttcgtaggta tttggattgc cttcgtgctg
      601 gtgctgttga tgatgatttg gggttttgca atcctgattc cgatcatccg cgatcagacg
      661 atagggatag tccgcgaatt tcctacttat tggcaggcaa tcgagagcat gacgattgaa
      721 ttgatcaact atgattggtt cacttccttg caggaacaga tcagcgaaat caatgcagat
      781 atcttcaata ccgtctccga gaagctgaac gaggtgttgt cgaacacggt cagcggatta
      841 ggcagcgttg tgggactttt gacgaatgcg tttgtaggaa tcgtcacgat gcctatcatc
      901 ttgtattatc tactgaagga aggagataaa ttgccactga cttttctgca aatatttcct
      961 acaaacctac gggagtcgat cggggacttg ctgaagaaag tcaacacgca gatcagccag
     1021 tatgtgcgcg gtcagattac ggtcgcgttc tttgtcgggt tgatgttcgt catcggttat
     1081 gccatcatcg gaatgaaatt cggaatcgtg cttgggatat tcgccggatt cctgaacatc
     1141 attccttata tgggttcctt cattgcgatg gtgcccgcgg tgattgttgc gatagtggat
     1201 tctccgctga tgctggcaaa agtgctgctg gttttctcaa tcgagcaatt cattgaagga
     1261 cgcgtcattt caccgcagat actcggcagc aatctcgagg tccatcccgt cacgatcatc
     1321 tttgtattgc ttacggcagg aaaactgttc ggtctgaccg gttttatctt gggtattccc
     1381 ggctatgctg ttcttaaagt gctgttcatg catatttttg aatggtacaa ggaaatatcc
     1441 ggattgtatt tggatgaacc tgaaatcgaa gaagagcctg aggaggatta tcttcaagaa
     1501 taatcgaaat gaaaagggga atgcttgatg aacaaaaaga gatggattgc ggtagcgatt
     1561 gcgctgattc ttttcgtggc atcgcttggt tcacaatttc tgtcgacccg gttggctgac
     1621 caaacggaag actccctcag ccagcttacc ggcaatctga taccaggaac gagcttgcag
     1681 gaaaacgtta tggaaagtgg cgatgccagc aatcggattg cggttctgca tatcgaaggg
     1741 acgattcaat caggtgcggg cagtgtgctg acggacggnn nnnnnnnntc cagttacgat
     1801 catgaccttt ttttggagca gttgaaagcc atcgaagagg atgacacaat caaagggatc
     1861 ttcttgatag tcgacagccc gggcggcggg gtctatgaaa gtgcggaggc gcacgataaa
     1921 ttgtaccgca ttgctgctga aaaggaactt ccggtctatg catccatgca aggaactgct
     1981 gcaagtgggg gctactacat ttccgctggt gcggacaaga tttatgccac tgctgaaacg
     2041 actaccggtt caatcggcgt catcatgcag gcgatcgaat actcagggct gcttgaaaag
     2101 ctgggtatcg aatacaacac aatcaagtcg ggcagcctca aggacatcgg gtcgccggat
     2161 cgggatatga cagaggaaga acgcgcgttg ctgcgagcct acgtcgatga ggcttatcag
     2221 cgtttcgtca ctgtcgtttc aacgggaagg gatatgccgg aagaagaggt ccgcgctgtt
     2281 gcgaatggca tgatttattc cggcacacag gctcaagcgc ttggattggt ggatgagata
     2341 gcctacacgg atgatgcatt gaaagctatg aaaaccgctt atggcctcga ggatgcggaa
     2401 gtattcgact atacttcgac acctggcctg ttttccgatt tttcttggtt gttcgctcag
     2461 gcggctgcca aactgacgag ccaggaaaat cagacgcttt ctgaactgca gttgctacgc
     2521 gacactttcg ggacaaccgc cgcgccgaga ttgctttatc tctacggagg tgagtgatga
     2581 tggaagagca aaccactacg catgaagaac gtttggaaga aatccgcaaa aagaaaatag
     2641 cctgggagga agccgaacgc tattccgacc agcaggaacg cccgtttcac cgctatccgg
     2701 attacgtatt cgccggtttt tggatcaggt tgtttgccta cctcgtcgac ttgctgattg
     2761 tgcaggcttt ggtggggatc ctgatcaaac cgattttcgc tttgggcggc atcagtatga
     2821 gcgagagttc tcccttaacc ttgtacggat ttattcagtt gctgctattt gtcgggtatt
     2881 tcatgctgac gacgaaatat acaaacggcc aaacagttgg gaaaatgatt ttcggcatcc
     2941 gtgtcgtctg cttcaaggaa gaaaacctca gttggcagac cgtcttgatc cgcgaaggtg
     3001 ttggccgcta cattggtaaa actgtggcag tgatctatct ggtcgctgct ttccaaagta
     3061 agaaacaaca tccgatcgat ttgctttgcg atacttcggt cgtgacggaa aacagtgtcc
     3121 gtgcgttgca agaaagttac tagtagagag aacaaatgtg gaaaaacggc atgcgaccgg
     3181 tttttatcgg tcgcatgccg ttttttgtgt tgtgtttggg gccgtataat aatgtggaac
     3241 tccggcgacg ccgtcgttcg ccggagtaga gcggtaaacc gcagaatgtc ctccggttag
//
LOCUS       DAOMUJ010000173         3298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8247_length_3298_cov_2.016343, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000173 DAOMUJ010000000
VERSION     DAOMUJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8247_length_3298_cov_2.016343"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLT73_05290"
     CDS             complement(<1..132)
                     /locus_tag="PLT73_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRM19828.1"
                     /translation="MALSKLENLYRHVILDHSSHPHNYGTLENADEQIELNNPTCGDV
                     "
     gene            complement(119..>590)
                     /locus_tag="PLT73_05295"
     CDS             complement(119..>590)
                     /locus_tag="PLT73_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM19829.1"
                     /translation="DFVYDQESTWSVLPWKFEAGTPNIAGGIGLGAAVDYLEAIGMDA
                     IEAYEKEMVRYVLPKLQAIEGITVYGPADPEKHTGVITFNLEGIHPHDLATAFDMEGI
                     AVRAGHHCAQPLMRYLDVSSTARASFYFYNTFEEADQFIASLHKIKEFFKDGFI"
     assembly_gap    591..600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<601..1338)
                     /locus_tag="PLT73_05300"
     CDS             complement(<601..1338)
                     /locus_tag="PLT73_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM19830.1"
                     /translation="MIDAKKIKQDFPILFQTVNDEPLIYLDNAATSQKPKQVLEAIQH
                     YYEYDNANIHRGVHTLAERATSAYEAAREKLRRFINAASEKEVLFTRGTTTGLNWVAV
                     GFGDLIVEEGDEIYITPMEHHSNVVPWQQLAKRKQAKLVYLPLTEDGFVDVEKSAEVI
                     TAKGKILSVCHSSNVLGVTNPIKELAALIHQHNGYIVVDGAQSTPHMKVDVQALDADF
                     YAFSGHKMLGPTGSGVLYGKEALLEKME"
     gene            complement(1335..>1739)
                     /locus_tag="PLT73_05305"
     CDS             complement(1335..>1739)
                     /locus_tag="PLT73_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM19831.1"
                     /translation="VTNYGKHSIGHILQHGVIMDRSTLTFNGIGHIIKGAKGADAQQE
                     SRVLMLSDNARGDANPILLIDENDVTAGHAASVGRVDPEEMFYLLSRGIPKAEAERLV
                     IRGFLGTVIAAIPVKEIRDGLVEMIERKLMKL"
     assembly_gap    1740..1749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1750..2633)
                     /locus_tag="PLT73_05310"
     CDS             complement(<1750..2633)
                     /locus_tag="PLT73_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM19832.1"
                     /translation="MLNEEKTSLLDAVRLFSIEKEEPEWMRNKRLEWLEKYEELALPL
                     MERISYHRWPLFDTTISAGAYGEELVNSQGLQYDLGDTPKIVQYGNVTLMEQLPQHLI
                     DQGVIFTDLYTAAQDYPELVQEYYMTTAVKPEEDRFTAFHTAFMNSGVFLYVPKNVVI
                     EEPLEALFIQNSHVKESFVKHVLLVADVNSSVKYVERYETEGNEKNSANIIVEVITKT
                     GAQVKFSAVDTLGENTSVYINRRGHLLKDSSIEWAIGVMNDGNVISDFDSDLIGEGSH
                     SEIKVVAVSTGRQVQGIDT"
     gene            complement(2645..>3298)
                     /gene="sufC"
                     /locus_tag="PLT73_05315"
     CDS             complement(2645..>3298)
                     /gene="sufC"
                     /locus_tag="PLT73_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRM19833.1"
                     /translation="GKSTLSQAIMGHPSYTITKGQVLLDGEDVTEMEVDERARAGLFL
                     AMQYPSEIAGITNAEFMRAAINARRPEDDKISVMNFIKKLDEKMEILNMPEEMAERYL
                     NEGFSGGEKKRNEILQLMMIEPKFAILDEIDSGLDIDALKVVSKGVNLMSGPDFGVLI
                     ITHYQRLLNYVHPTFVHVMMDGRIVKSGDASLAKRLEAEGYRGIRDELGLDIQLDEE"
BASE COUNT          681 a          832 c          875 g          890 t
ORIGIN      
        1 gacgtcgccg caggtcgggt tgttcagttc gatctgctcg tcggcgtttt ccaaggtgcc
       61 gtaattgtgc gggtgactgg agtggtcaag aatgacatgg cggtataggt tttctagttt
      121 agataaagcc atctttgaaa aactccttta ttttatgcaa ggaggcgatg aattgatcgg
      181 cctcttcgaa agtgttgtag aaatagaaac tcgcccgggc tgtcgaggat acgtccaaat
      241 aacgcatcaa cggctgcgcg cagtggtggc cggcgcggac agcgatgcct tccatgtcga
      301 aagccgtagc caagtcatgc ggatgtatcc cttctaggtt gaaagtgatg acgcccgtgt
      361 gcttttccgg atcggcagga ccgtagacag tgatgccttc gatcgcctgc agtttcggca
      421 gcacgtagcg aaccatttcc ttttcatagg cttcgatggc atccatgccg atcgcttcca
      481 aatagtcgac ggcagctcca aggccgattc cgccggcgat gttcggtgtg cccgcttcga
      541 atttccaagg caggacactc caggtgcttt cctggtcata aacgaaatcg nnnnnnnnnn
      601 ctccatcttt tcaagcaagg cttccttgcc gtaaagcacg ccggatccgg taggcccgag
      661 catcttgtgg ccgctgaagg cgtagaagtc ggcgtccaat gcctgcacat cgactttcat
      721 gtgcggcgtc gattgggcgc cgtccacgac gatgtagccg ttgtgctggt ggatcaatgc
      781 ggccagttcc ttgatcgggt tagtgactcc caacacgttc gaggaatggc agaccgacag
      841 aattttgcct tttgcagtga tgacttcagc cgatttttcg acatcgacga agccgtcttc
      901 tgttagcggc aagtagacta atttggcctg cttgcgtttc gccaactgtt gccaaggaac
      961 cacgttggag tggtgttcca tcggcgtgat gtagatttca tcgccttctt cgacgatcag
     1021 atcgccgaag ccgacagcaa cccagttcag gccggttgtc gtgccgcggg tgaaaaggac
     1081 ttccttctct gaagcggcat tgatgaagcg gcgcagcttt tcgcgggcgg cttcgtaagc
     1141 tgaagtcgcg cgttccgcca acgtatgcac gccgcggtgg atattggcat tgtcatactc
     1201 gtaatagtgt tggatggctt ccagcacctg tttcggtttt tgggaagtgg ccgcattgtc
     1261 cagataaatc agcggctcgt cgtttacggt ctggaaaagg atcgggaagt cttgtttgat
     1321 tttctttgca tcgatcaaag tttcatcaac ttcctttcga tcatctccac caaaccgtca
     1381 cggatttctt tgaccgggat ggccgcgatg accgtaccca ggaatccgcg gatgaccaga
     1441 cgttctgctt ctgccttagg gataccgcgg gaaagaagat agaacatctc ttccggatcg
     1501 acgcgtccga cgctggcggc atggccggct gtgacgtcgt tctcgtcgat caacaggatc
     1561 gggttcgcat cccctctggc gttatcggac agcatcagga cacggctttc ttgttgcgca
     1621 tctgcaccct tcgcgccttt gatgatgtgg ccgatgccgt tgaatgtcaa agttgaacgg
     1681 tccatgatga ccccgtgctg caaaatatgg ccgattgaat gtttgccgta gttcgtgacn
     1741 nnnnnnnnng tatcgatgcc ctgcacttgt ctgccggttg aaactgccac gactttgatt
     1801 tcactgtggg agccttcacc gatcagatcg gaatcgaaat cggagatgac attgccatcg
     1861 ttcatgacgc cgatcgccca ctcgatggag gaatctttca acaggtggcc gcggcggttg
     1921 atgtagacgg atgtgttttc gcccaaagtg tcgactgcag agaatttgac ttgcgcgccg
     1981 gttttcgtga tgacttcgac gatgatgttg gcgctgttct tttcattgcc ttcagtttca
     2041 tagcgctcca cgtatttgac ggagctgttc acgtcagcga ccagaagcac gtgtttgacg
     2101 aagctttcct tcacgtggga gttttggatg aacagagctt ccaacggctc ttcgatgacg
     2161 acgttcttcg gcacataaag gaagacgccg ctgttcatga aggctgtgtg gaaagcagta
     2221 aaacgatctt cttccggttt cacggctgtc gtcatatagt attcttggac cagttcagga
     2281 taatcctgag cggctgtgta taaatcggtg aaaatgacgc cttgatcgat caaatgctgc
     2341 ggtaattgct ccatcagcgt cacatttccg tattgtacga ttttcggtgt gtcgcccaaa
     2401 tcatattgga gaccttggct gttcaccaat tcctcgccgt atgcaccggc ggagatggtc
     2461 gtgtcaaaca gcggccaacg gtggtagctg atgcgttcca tcaatggtaa agccagctct
     2521 tcgtattttt caagccattc caaacgttta ttgcgcatcc attccggttc ttccttttca
     2581 atcgaaaaca ggcgaaccgc atccaacaag gacgtttttt cttcgttcag catgtgacgt
     2641 cctcctattc ttcatccaat tggatatcta gacctaattc gtcgcggatg ccgcggtagc
     2701 cttccgcttc cagacgtttc gccaagctgg cgtcgccgga tttcacgatg cggccgtcca
     2761 tcatgacgtg gacgaatgta gggtgtacat aattcaagag acgttggtag tgggtgatga
     2821 tcaggacgcc gaagtcagga ccggacatca gattgacgcc tttggaaacg actttcagag
     2881 catcgatatc caaaccggag tcgatttcgt caaggatcgc gaatttcggt tcgatcatca
     2941 tcaattgcag gatttcgttg cgtttctttt ctccgcctga aaagccttcg ttcaaatagc
     3001 gttcggccat ttcttccggc atattcagga tttccatttt ttcgtccaat ttcttgatga
     3061 aattcatgac cgaaattttg tcgtcttccg ggcggcgggc attgatggcc gcgcgcatga
     3121 attcagcgtt tgtgatgccg gcgatttcgc tcggatattg catcgccagg aacaggcctg
     3181 cgcgtgcgcg ttcgtcgact tccatttccg tcacatcttc gccgtccaag agaacttggc
     3241 cttttgtgat tgtgtagctc ggatgaccca tgattgcttg tgacaaagtc gatttacc
//
LOCUS       DAOMUJ010000174         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8258_length_3294_cov_3.150046, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000174 DAOMUJ010000000
VERSION     DAOMUJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_3294_cov_3.150046"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..857
                     /locus_tag="PLT73_05320"
     CDS             93..857
                     /locus_tag="PLT73_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM19834.1"
                     /translation="MARIIAVANQKGGVGKTTTTVNLAASLAYLGKKVLLIDSDAQGN
                     ATSGLGISKADVEKDIYDVLVNQEPIANVIRESSRENLWVVPATIQLAGAEIELTNQP
                     HREARLYEAVQSLREEYDFIFIDCPPSLGHLTINAFTASDSVLIPVQCEYYALEGLSQ
                     LLNTFRLVQKHFNKDLKLEGVLLTMLDARTNLGFEVVDEVKKYFKEKVYKTIIPRNVR
                     LSEAPSYGQSIVDYDIRSKGAEVYLELAKEVLADGE"
     gene            847..1740
                     /locus_tag="PLT73_05325"
     CDS             847..1740
                     /locus_tag="PLT73_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM19835.1"
                     /translation="MANKNSKGLGRGIDALFSNFEDIEKIDEKTEKVQEIPLDEIRPN
                     PYQPRKTFDDSTLRELADSIMLNGVIQPVILRQSSVKGYEIIAGERRVRASRLAGKTT
                     IPAIVREFDETAMIEVAILENLQREDLTPLEEAEGYQTLMNKLNLTQVDVAQRLGKSR
                     PYIANHLRLLNLPQDVKGMLQEGLISMGQARTLLGLNDESLISELAKRAAEEGITVRH
                     LELLVQQLNNPGDVAKKKNKKKKETKPVYIKESEERLMDKFGTSVQISSKGEKGKIEI
                     EYLSPADLTRILDILQISLDD"
     gene            1778..1984
                     /locus_tag="PLT73_05330"
     CDS             1778..1984
                     /locus_tag="PLT73_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRM19836.1"
                     /translation="MVQQDYGLHDVVEMKKPHPCKSNSWEIIRMGMDIRIKCLKCGQM
                     VMMPRREFEKKMKKVLTKAEETVE"
     gene            2036..3136
                     /gene="ychF"
                     /locus_tag="PLT73_05335"
     CDS             2036..3136
                     /gene="ychF"
                     /locus_tag="PLT73_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRM19837.1"
                     /translation="MSLTAGIVGLPNVGKSTLFNAITKAGVEAANYPFATIDPNVGVV
                     EVPDERLNKLTELVKPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIRQVDAICH
                     VVRCFQDDNITHVSGKVDPLDDVETINLELIFADLESIEKRYTRISKIARTKDKDAVR
                     ELEILEKIKTTLEEGKSARTIEFTPEEEPLVKSLFLLTTKPVLYVANISEEDVIAGGD
                     NEMVQAVREFAATEGAEVVTVCARIEEEVAELDDEEKAEFLAELGIKESGLDQLIRKA
                     YTLLGLGTYFTAGEKEVRAWTFRLGMKAPQTAGIIHTDFERGFIRAETVSYADLLAYG
                     SMQAAKEAGKVRLEGKDYVVQDGDVMEFRFNV"
BASE COUNT         1025 a          700 c          850 g          719 t
ORIGIN      
        1 gcgcaggtat cgttttttct ctttttttag gcaaaaaagt agaacggcgg cacagtagct
       61 tcaaatgaaa gacagaaagt ggaggagaac agatggcacg gattattgca gtcgcaaatc
      121 agaaaggcgg cgtcggaaaa acgacgacca ccgtcaattt agcagcgtct ttggcctact
      181 taggcaaaaa agtgttattg atagatagtg atgcccaagg gaacgccacg agcggactcg
      241 gtatttcaaa agcggatgtt gaaaaagata tatatgatgt attagtgaac caagagccta
      301 tcgcgaacgt catcagagaa tcctcacggg aaaatttatg ggtcgtaccg gcaaccatcc
      361 aattggcagg cgccgaaatc gagttgacga atcagccgca tcgtgaagcg cgcctttatg
      421 aggcagtgca gtcattgcgt gaggaatacg acttcatttt tattgattgc ccgccctcac
      481 tgggacattt gacgatcaat gccttcaccg ccagtgattc ggttttgatt ccggttcagt
      541 gcgaatacta tgctttggaa gggttgagtc aactgttgaa caccttccgc ttggttcaga
      601 agcatttcaa taaggatctg aaattggaag gggtattgtt gacgatgctg gacgctcgga
      661 caaacctcgg ctttgaggtc gtggatgagg tcaaaaagta tttcaaagag aaagtataca
      721 agacaatcat tccgcgcaat gtccgtttat cggaagcgcc aagctatgga caatcaatcg
      781 ttgactatga tatccgttca aaaggagcgg aagtttatct tgagttggca aaggaagtgt
      841 tagcagatgg cgaataaaaa cagcaaaggg ctaggacgcg gcatcgacgc gctcttcagc
      901 aactttgaag atatcgaaaa aatcgatgaa aagacagaga aggtacagga gatcccttta
      961 gatgagatcc gtcccaatcc ttatcagcca cgaaaaacct ttgatgattc gactttgcgg
     1021 gaattagctg actcgatcat gctgaacggt gtcatccagc cggtcatcct gcgccaatcc
     1081 agtgtgaagg gttatgaaat cattgcaggc gaacgccgcg tccgcgcgtc ccgtttggcc
     1141 ggcaaaacaa caatcccggc aatcgtccgt gaatttgacg aaacagccat gatcgaagtc
     1201 gccattctgg aaaatctgca gcgggaagat ttgactccgc tggaagaggc agaaggctac
     1261 caaaccttga tgaacaagct gaatcttacg caagtggatg tcgcccaacg gttaggcaag
     1321 agccgcccgt acatcgccaa ccatctgcgc ttgttgaatc tgcctcagga cgtcaagggg
     1381 atgctgcaag agggattgat ctctatgggg caagcccgta cgcttttggg tttgaacgat
     1441 gaatcgttga tcagcgaact ggcaaaacga gctgccgaag aaggcatcac ggtccgacac
     1501 ttggaattgt tggttcaaca attgaataat cccggtgatg tcgccaaaaa gaaaaataaa
     1561 aagaaaaaag aaacgaagcc ggtatacatc aaagagagtg aagaacgttt gatggacaaa
     1621 ttcgggacat ccgtacaaat atccagtaag ggtgaaaaag gcaaaatcga gatcgaatac
     1681 ctttctccag ccgacctgac ccggattttg gatatcctgc aaatttcgct ggacgattaa
     1741 gaatagacga agacatcaga aagaggtgca tttggccttg gtacaacaag attatggatt
     1801 gcatgatgtg gtcgaaatga aaaaaccgca tccttgcaaa agcaactcat gggaaatcat
     1861 ccgaatgggt atggacatcc gcatcaaatg cctgaaatgc gggcagatgg tcatgatgcc
     1921 gcgccgcgaa ttcgagaaaa aaatgaaaaa agtactgact aaggcagaag aaactgtcga
     1981 ataggtcttt gaacgaacaa atgaaaagca atataagtgg aaagagtgtg aagaaatgtc
     2041 attaacagca ggaatcgtag ggttaccgaa cgtcgggaaa tccactttat tcaacgccat
     2101 caccaaagcg ggagtggaag ccgcaaacta tccattcgca acgatcgacc ccaatgttgg
     2161 agtagtcgaa gttcccgatg aacgtttgaa taaattaaca gaattagtga aacctaaaaa
     2221 aaccgtcccg acaacttttg aatttacgga tatcgccggc atcgtaaaag gcgccagcaa
     2281 aggggaaggt ttggggaata aatttttggc caacatccgc caagtggatg ctatctgtca
     2341 tgttgtccgt tgtttccaag acgataacat cacgcacgta agcggtaaag tggatccctt
     2401 ggatgacgtc gaaaccatca acctggaact gatttttgcc gacttggagt ccatcgagaa
     2461 acgctataca cgcatcagca aaattgcccg cacaaaagac aaagatgctg tgcgcgaact
     2521 ggaaatcctg gaaaaaatca agacgacttt ggaagaaggc aaatccgcgc gtacgattga
     2581 attcacaccc gaagaagaac cgcttgtgaa gagcttattc cttttgacga caaagcctgt
     2641 tctgtatgtc gcaaacatct ccgaagagga cgtaatagcc ggcggagaca atgaaatggt
     2701 tcaagctgtc cgtgaatttg cggcaacaga aggcgcagag gtcgttaccg tttgcgcccg
     2761 tatcgaagaa gaagtggctg aactggatga cgaagaaaaa gctgaattcc tggcggagtt
     2821 gggcatcaaa gaatccggat tggatcaact aatccgtaaa gcttacacgt tgcttggcct
     2881 gggaacatat tttacggcag gcgaaaaaga agttcgtgcc tggacattca gactagggat
     2941 gaaagcacca caaacggcag gaatcatcca taccgacttt gagcgcggct tcattcgtgc
     3001 cgaaacagtt tcctacgctg acctgttggc ttacggcagc atgcaagccg ccaaagaagc
     3061 agggaaagtc agactagaag gaaaagacta cgtagtccaa gacggcgacg taatggaatt
     3121 ccgattcaac gtataacagg atgtgatgat tcgcgtttag atacccggac gttggagcac
     3181 ccagcgaagt gaacctcttt tgttcactac gctgggtgtg aaacgggagc ggtatctgcg
     3241 aatcagaact caactaaagg atacgatgaa atgcgggagg ggatgagcac taag
//
LOCUS       DAOMUJ010000175         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8267_length_3292_cov_1.924930, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000175 DAOMUJ010000000
VERSION     DAOMUJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8267_length_3292_cov_1.924930"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLT73_05340"
     CDS             complement(<1..266)
                     /locus_tag="PLT73_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HRM19838.1"
                     /translation="MRLRICHLYGNLLNTYGDNGNLLMLQYCAREKGVDFETEIISLD
                     QPFDPNKYDLVFFGGGQDYEQFIVSKDIQAKAAGIKAYIENDGVL"
     gene            complement(267..1619)
                     /locus_tag="PLT73_05345"
     CDS             complement(267..1619)
                     /locus_tag="PLT73_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRM19839.1"
                     /translation="MTIRGSLAINIGKIAQWGLQTFTKGGTSLPGKLAVKLDPDVLQH
                     LSENFDVVIITGTNGKTLTTSLTYHVLQQKYPHILTNPTGANMEQGIISSFLEGYSRK
                     VKKERAFAVLEVDEASLVKVTKFIKPKVIVNTNVFRDQMDRFGEIYTIYQKMVDGAAL
                     APDAVILANGDSPIFNSKELVNKQIYFGFNHEPDGDTLAHYNTDGVLCPKCQHILRYK
                     FNTYSNLGKYYCPHCDFKRPELTYAVTKVDKLTHKSSSFEIDGHPFQTNVAGLYNVYN
                     ALAAYSVGKHFGLTPEEIQAGFSAAQQKFGRQETITVNDKEIILNLIKNPVGLNQVID
                     LLHYEEEPFSVVSILNDRPADGTDVSWIWDGNYEKFLDFDIPKVTVSGIRQKELTLRM
                     KVAGIPEDKLEVVPEISDVIKTFKNAPTKKIYVMATYTAVLQLRKELANQGYIAERMK
                     "
     gene            1995..2567
                     /locus_tag="PLT73_05350"
     CDS             1995..2567
                     /locus_tag="PLT73_05350"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRM19840.1"
                     /translation="MAQLFFKYGAMNSGKSIEILKVAYNYEEQNKSVMIFTSALDDRD
                     GIGYVSSRIGLKREAIPIADDMDVFLEVAKNPKRPACILIDESQFLSKAHVIQLARIV
                     DELAIPVMAFGLKNDFQNELFAGSKYLLLYADKIEEIKTICWYCHKKAIMNMRVVDGK
                     PVYAGEQIQIGGNESYLPVCRNHYHHPPLA"
     gene            2610..>3292
                     /locus_tag="PLT73_05355"
     CDS             2610..>3292
                     /locus_tag="PLT73_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRM19841.1"
                     /translation="MYDQLDAFIIRYEELSELLSDPEVLSDTKRFLELTKEEAGLREK
                     VATFKRYKEVEGEIADTEEMLSENLDEDMAAMAKEELSDLKKEKTELEERIKVLLLPS
                     DPNDDKNIIMEIRGAAGGDEAALFAGTLFTMYSKFAESQGWRVEVMDANITGIGGYKE
                     IILMISGQGAYSKLKYESGAHRVQRVPETESQGRVHTSTATVVVMPEAEDVELNIEDK
                     DIRTDIYHA"
BASE COUNT          893 a          681 c          820 g          898 t
ORIGIN      
        1 agaacgccat cgttttcgat gtatgctttg attcctgcag ctttagcttg aatgtctttg
       61 gagacgatga actgttcgta atcctgacct cccccaaaaa agacgagatc atacttattg
      121 ggatcgaacg gttggtccaa gctgatgatt tcggtttcga aatcgacgcc tttttcccgg
      181 gcgcagtact ggagcatcaa aagattgcca ttgtcgccgt atgtattcag taggttgccg
      241 tacaagtggc aaatacgtag tcgcatttat ttcatccttt ccgctatata gccctggttc
      301 gccagctctt tacggagttg cagcacagcg gtataagttg ccatcacata gatttttttc
      361 gttggagcat ttttgaaagt tttgatgaca tcggagattt ccggaaccac ttccagctta
      421 tcttccggaa tgccggcaac cttcatccgt agggtcagct ctttttggcg gatgccgctg
      481 acggtcactt tcgggatatc aaaatccaaa aatttctcgt aatttccgtc ccagatccag
      541 ctgacatcgg ttccgtctgc cgggcggtca ttcaggatcg acacgacgct gaatggttcc
      601 tcttcgtagt gcaagagatc gattacctga ttcaggccga ctggattttt gatcagattc
      661 aggatgattt ccttgtcgtt cacggtgatc gtttcttgac gtccaaattt ttgttgtgcc
      721 gccgagaaac cggcctggat ctcttcgggc gtcaatccga aatgcttgcc gaccgaataa
      781 gcagccaagg cattgtagac attgtagagt cccgcaacat ttgtctggaa cggatgacca
      841 tcgatctcaa aagaagagct tttgtgcgtc agcttatcga ctttggtgac agcataagtc
      901 aattctggcc gtttgaaatc gcaatgcggg cagtagtatt tcccaaggtt gctgtaggta
      961 ttgaatttat agcgcaaaat gtgttggcat ttcggacaaa gaacgccgtc cgtgttgtaa
     1021 tgcgccaagg tgtctccatc cggttcatga ttgaagccga aatagatttg tttattgacc
     1081 aactcttttg agttgaagat cgggctgtcc ccattcgcga gaatgacggc atcaggtgcc
     1141 aatgcagcgc catcgaccat cttctggtag attgtataga tttctccgaa tcggtccatc
     1201 tgatcgcgga aaacattcgt attgacgatg actttcggct tgatgaactt cgtaacctta
     1261 acgaggctgg cttcgtcgac ctcaaggaca gcaaaggctc tttccttttt gactttgcgc
     1321 gaatagcctt ccaggaaaga ggaaatgatg ccttgctcca tgttggcccc cgttggattc
     1381 gtcaaaatat gcggatattt ttgctgtaga acatgataag tcaaagatgt cgtcaatgtt
     1441 ttgccgttcg tgccggtgat gatgacaaca tcaaaatttt cggaaagatg ttgcaacacg
     1501 tcgggatcca atttgacggc cagtttgccg ggcagacttg ttccgccttt ggtgaatgtc
     1561 tgaagacccc actgggcgat cttgccgata ttgattgcta gtgaaccgcg gatagtcaaa
     1621 taaattcgct ccttacttat gtttctcttg ccatcatagc atataacaag gcttttttcg
     1681 atagcgttag tatagcagaa tttaccggga atgaatattt ttagattacg cctcgtgtca
     1741 gaaacggggc tttcgattgc tgaaggacag tcggtaatcg tgaattttat tgactttgca
     1801 gccatttcct actatcatta tacgggatgt tattcagttt tttccgctcc ggtaatctta
     1861 atcggagcgg aatgatgaag gtgagataat agcggggtcg gaagcaggat gacataagtg
     1921 catcctttct ttttgcaagg aattccgatc gatggatgaa aagtaggcaa gaggctgttt
     1981 gaaaggtgga catgatggca cagttatttt ttaaatacgg ggcgatgaac agcgggaaat
     2041 cgatcgagat cctgaaggtc gcctataatt atgaagagca gaacaagtcg gtaatgattt
     2101 tcacaagcgc acttgatgat cgtgatggta tcggatatgt ttctagtcgg atcggattga
     2161 agcgcgaagc tatcccgatt gcagatgata tggatgtatt cttggaagtc gccaaaaatc
     2221 cgaaaagacc cgcttgtatc ttgatagatg aatcccaatt cctgtccaag gcgcatgtca
     2281 tccaactggc gcgcatcgtc gacgaactgg cgattccggt gatggcgttc ggattgaaga
     2341 atgattttca gaacgaattg tttgcaggct cgaaatatct gttgctttac gcggataaga
     2401 tagaagagat aaaaaccatc tgctggtatt gtcataaaaa agcaatcatg aatatgcgtg
     2461 tcgtggatgg aaaaccagta tatgcaggtg aacaaatcca gatcggcgga aatgaatcct
     2521 atcttcccgt ctgtcgcaat cattaccatc atccgccatt ggcctaaaac aaataatcag
     2581 aaacaaaaaa agaggggttt gaaaaagtta tgtatgatca attagatgca ttcattatcc
     2641 gttatgaaga actttccgaa ttgttgagcg atccggaagt tctttctgat acaaaacgat
     2701 tcctggaatt aactaaagaa gaagcagggc tgagggagaa agttgcgacc ttcaagcgtt
     2761 acaaagaagt ggaaggcgaa atagccgata ccgaagagat gttgtcggaa aatttggacg
     2821 aggatatggc ggctatggct aaagaagaat tgagcgactt gaagaaagaa aaaacggaac
     2881 ttgaggagcg cattaaagtc ttgttgttgc ctagcgatcc gaacgatgac aaaaacatca
     2941 tcatggaaat ccgaggggct gccggagggg atgaagctgc actgttcgcg ggtacgttat
     3001 tcacgatgta cagcaaattt gcagaaagcc aagggtggag agtcgaggtc atggacgcga
     3061 atatcaccgg catcggtggt tacaaagaaa tcatcctgat gatcagcggc caaggagctt
     3121 actccaaact gaaatatgag agtggcgcgc accgtgtcca acgtgttccg gaaacagaat
     3181 cacaagggcg tgtgcatacg tcaactgcta ccgtagtcgt catgccggaa gcggaagatg
     3241 ttgaattgaa catcgaagat aaagatatcc gcacagatat ctaccatgcc ag
//
LOCUS       DAOMUJ010000176         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8311_length_3278_cov_2.290102, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000176 DAOMUJ010000000
VERSION     DAOMUJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_3278_cov_2.290102"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1174
                     /locus_tag="PLT73_05360"
                     /pseudo
     CDS             94..1174
                     /locus_tag="PLT73_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            1190..1921
                     /locus_tag="PLT73_05365"
     CDS             1190..1921
                     /locus_tag="PLT73_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRM19842.1"
                     /translation="MKVTVWNEYRHEKTDEAVAKVYPKGIHGQIESFLKEAGLDVKTA
                     TLDEPEHGLTEAVLAETDVLVWWGHIAHNEVTDEVVNRVHEHVLKGMGLVVLHSAHMS
                     KIFMKLMGTSCDLKWREAEEKERIWVVNPSHPIAEGIGEYIELEKEEMYGEHFDVPTP
                     DELIFVGWYEGGNVFRSGMTYRRGNGKIFYFQPGHETYPTYYNKDIQKVIINGIDWCR
                     PSKRAYPAYGNSQALEEIKNGQQPV"
     gene            1973..2941
                     /locus_tag="PLT73_05370"
     CDS             1973..2941
                     /locus_tag="PLT73_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003729565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRM19843.1"
                     /translation="MKLGVFTPLFANLSFEDMLDKVQRAGLNAVEIGTGGNPGNHHCP
                     TDELLESEEKRAAYLDELKKRNLTISAFSCHSNPISPDPVEAKASDEILRKTIRLAAL
                     MGVPVVNTFSGTAGDSEEATAPNWPVIPWPEVYTDIYKWQWENKLIPYWKEIGEMASE
                     YGVKIGIELHGGFLCHTPYTLLKLRENTCDAIGVNLDPSHLWWQGIDPVGAIKILGEA
                     GAIHHFHAKDTYLDQDNINMYGLLDMQPYGDVKTRAWTFRSVGCGHDVKVWSDMMSAL
                     RMYGYDYVVSIEHEDPLMSIDEGLNRAVTNLQSVLIKETPHAMWWA"
     gene            2957..>3278
                     /locus_tag="PLT73_05375"
     CDS             2957..>3278
                     /locus_tag="PLT73_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM19844.1"
                     /translation="MKKLKVTIIGYGGMGSFHAKLIQANPEVEVHGIFDILESRQQAG
                     EADGYSAYVSLEEALDDETVDAILIATPNDVHKEIAVQALEAGKHLICEKPVTLTSEE
                     LSEIM"
BASE COUNT         1017 a          605 c          898 g          758 t
ORIGIN      
        1 gtattggatg acacgaatgt attatcacca atcctatcaa aaaggacggg tctatcccgt
       61 taaaatgaga ataatagaag gagagtcaat aatatgatca aaattggaat tatcggttgt
      121 ggaggaattg caaacaacaa acatatgcca gctttggcta aagtggaaac tgttgaaatg
      181 gtggcatttt gtgacattat cgaggaacgc gcaagcaagg cgagagaggc gtttgggacg
      241 gatgctgcaa atgtctatac ggactatatg gagcttttaa aaaatgatga aattgatgtc
      301 attcatgtct gcacaccaaa tatttctcat gctgaaatct caattgcggc aatggagtcc
      361 ggtaaacatg tgatgtgtga aaaaccgatg gctaaaactt cggaagaagc agaagctatg
      421 atagccgcga tgaatcggac cgggaaaaag ctgacgatcg gttatcaaaa tcgattcaga
      481 aaagattctt cttatttgca taaaatctgt caaaagggag aattgggaga tatttataac
      541 gcgaaagcgc atgcgatcag gagacgggcg gttcctactt ggggcgtatt tcttgatgaa
      601 gaagcgcaag gtggtggccc tttgattgat atcggaacgc acgcgcttga tttaacgttg
      661 tggatgatga ataactataa accaaagtat gttgtgggga atacgtatca taaattggca
      721 cagacggaga atgccgcaaa tgctttcgga ccttggaatc cagctgaatt taccgtagag
      781 gattccgctt tcggctttat cgtaatggag aacggagcaa ccatttatct ggaatccagg
      841 ggaggctcaa acgactctgc acgggactca aggcggggct gatatgaagg atggcttgcg
      901 catcaacgga gaatctgatg gcatgatgta cgataaaaca atggtgctgg agcctggtgg
      961 agtagacttc tacgacggat cgggagatga tccggcctat ctggaagcga aacaatggat
     1021 tgagagcatc caaaacgata cagaaccggt tgtgtcacct gaacaggcat tggtcgtgac
     1081 ccaaatactg gaagctattt atcaatctgc caagaccgga gcgccggtat acatcaatca
     1141 acataaagaa gagttgggaa aaatactggt ctgaagaggg gataatcgaa tgaaagtaac
     1201 ggtatggaac gagtatcggc atgaaaaaac ggatgaggct gtagcaaaag tctatcccaa
     1261 gggaatacac gggcaaatcg aaagtttctt gaaggaagca ggtttggatg taaagacagc
     1321 tacactggac gaaccggagc acggcttgac ggaagcggtc ttagcagaaa ccgatgtgct
     1381 tgtctggtgg ggacacattg cccataacga agtaacggat gaagtagtca accgcgtcca
     1441 tgaacatgtc ctgaaaggga tggggctggt tgttttgcac tcggcgcata tgtccaaaat
     1501 ttttatgaaa ttgatgggca caagctgcga cctgaaatgg cgtgaagctg aagaaaagga
     1561 gcggatttgg gtagtgaatc caagccatcc aatcgctgag gggatcggcg aatacatcga
     1621 gttggaaaaa gaagagatgt acggcgagca ctttgatgtg ccgacgccgg atgaattgat
     1681 ttttgtcgga tggtatgaag gcggaaatgt tttccgcagc gggatgactt atagacgcgg
     1741 caacgggaag attttttatt ttcagcctgg tcatgagaca tacccgactt actacaataa
     1801 agatatccaa aaagtcatca taaacggaat cgattggtgt agaccgtcaa agcgggcgta
     1861 tcctgcttac ggaaacagcc aagcgttgga agaaataaaa aatgggcagc aacccgtata
     1921 aaaagagggt caatgctgac aaatcatgaa atgaagagag cgaggaatcg gtatgaaact
     1981 aggtgtattt acccccttat ttgcaaattt aagttttgaa gacatgctgg ataaagtcca
     2041 gcgagcaggt ttgaacgcag tggagatcgg aaccggtggg aatcccggga accatcactg
     2101 tcccacagat gaactattgg aaagtgaaga gaagcgagca gcgtacttgg atgaactgaa
     2161 aaaaaggaac ttaacaatca gtgcattcag ctgccacagc aacccgattt caccagatcc
     2221 tgtagaggca aaggcgagcg atgaaatact aagaaaaacc atcagattgg ctgcgttgat
     2281 gggggtacca gtcgtcaata ctttttccgg aacggcaggc gacagtgaag aggcaacagc
     2341 accgaattgg ccggtcattc cgtggccgga agtttacacc gatatttata aatggcaatg
     2401 ggaaaataag cttattcctt actggaaaga aatcggagaa atggccagcg aatatggcgt
     2461 caagattggc atcgaattgc acggcggttt cttatgccac acaccgtata cgcttttgaa
     2521 attgcgcgaa aatacctgtg atgcaatcgg cgtgaacctc gatcccagcc atttgtggtg
     2581 gcaagggatt gatccggtcg gcgccattaa gattctgggt gaagccggag cgatccatca
     2641 tttccatgcg aaagacacct atctcgatca ggacaacatc aatatgtatg gtctgttgga
     2701 tatgcaaccg tatggagatg tcaaaacgag agcatggacg ttccgatcgg ttggctgcgg
     2761 tcacgatgta aaagtatggt cagatatgat gagtgcgttg cggatgtatg gttatgatta
     2821 tgtggtcagc attgaacatg aggacccttt gatgtccatc gatgaaggat tgaacagagc
     2881 tgttacgaat ctgcaatctg tattgattaa agagacccca cacgctatgt ggtgggcgta
     2941 gaaaagagga ttaacgatga agaaattaaa agtcacgatc attggttacg gaggcatggg
     3001 cagctttcac gctaagctga ttcaggcaaa tccggaagta gaagtgcatg ggatattcga
     3061 tattttggaa agtcggcaac aggctggcga agcggatggc tatagtgcct atgtcagtct
     3121 tgaagaggct ttggatgatg aaacggtgga tgccatattg atcgcaactc cgaatgatgt
     3181 acacaaagag atcgctgtcc aggctttgga ggcaggaaaa catctcattt gtgaaaagcc
     3241 cgtgacactg accagcgaag agctgtcgga aataatgc
//
LOCUS       DAOMUJ010000177         3245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8407_length_3245_cov_2.357994, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000177 DAOMUJ010000000
VERSION     DAOMUJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_3245_cov_2.357994"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLT73_05380"
     CDS             <1..244
                     /locus_tag="PLT73_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRM19845.1"
                     /translation="ITALLVYAVRKYNLPKWCLILPIIFIPGIVVPIYLSQLLAIPFL
                     PLVVSLCIGQSVPAFVGYFLVQALEKRFYKSALLKS"
     gene            263..763
                     /gene="queF"
                     /locus_tag="PLT73_05385"
     CDS             263..763
                     /gene="queF"
                     /locus_tag="PLT73_05385"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRM19846.1"
                     /translation="MTAGRNEADLNGITQLGNQQTKYPQDYDPKVLETFPNKHPDNDY
                     IVKFNCPEFTSLCPMTGQPDFATIYISYVPGEIMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMKDLIALMAPKYIEVWGKFTPRGGISIDPYCNYGKPDTRWADVAWQRLAQHDLYP
                     EKVDNR"
     gene            773..1432
                     /gene="queC"
                     /locus_tag="PLT73_05390"
     CDS             773..1432
                     /gene="queC"
                     /locus_tag="PLT73_05390"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRM19847.1"
                     /translation="MNGTTQKQALVIFSGGQDSTTCLIQAIQKYGAANTVALSFSYGQ
                     RHGIELERAAWIANDLGVEHHILDASVMGKVTTNALMDESQTIKEADAEDYPNTFVDG
                     RNALFLLLAGIFAKSKDIRIIILGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNV
                     ETPLMYLTKAQTWELADQLGCLAYIEEHTHTCYMGVPGGCGECPSCKLRNAGLAEYLN
                     K"
     gene            complement(1566..>2052)
                     /locus_tag="PLT73_05395"
     CDS             complement(1566..>2052)
                     /locus_tag="PLT73_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019777119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRM19848.1"
                     /translation="KTQVTVEYADGHVKRIEAVVIACQHDESVDQERIREDMLRMVIP
                     AVIPAELLDDQTKYFVNAIGVARPVSVRVETFGTASLAEEQIEELIKKHFDLHPAAII
                     RDFDLRKPIYRKLACYGHFGRPELDLPWERTDKADLLADEMKALNKDMENQEGNAGQG
                     Q"
     assembly_gap    2053..2062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2063..2558)
                     /locus_tag="PLT73_05400"
     CDS             complement(<2063..2558)
                     /locus_tag="PLT73_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013439954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRM19849.1"
                     /translation="MKRTFFTSESVMEGHPDKLCDQIADGILDAILKEDPFARVACDV
                     SASTGLITVFGQITTVSTVDIPAIVRSIIQAVGYTDSDFGIDGKACSVLIGLDRQSPD
                     IAAGVGSSLEKRLGSDDEADQLGAGDQGLMFGYACKETPELMPLPIMLAHRLAKKVAE
                     LRKSG"
     gene            complement(2624..3091)
                     /gene="amrB"
                     /locus_tag="PLT73_05405"
     CDS             complement(2624..3091)
                     /gene="amrB"
                     /locus_tag="PLT73_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRM19850.1"
                     /translation="MKPHEGHGFEVPMHYLDRQLPPNLETEKILVTVPSYEHYYEWGK
                     RYGEAYDRSDLRTAIIASADLSHVLKPEGPYGYHSAGPLFDKLVVKAIKEKDAGFLER
                     AAECGLRSILFLMGAFEGRAYEAEVLSYEGPFGVGYLVATFMPSESEEQIKDA"
BASE COUNT          808 a          896 c          755 g          776 t
ORIGIN      
        1 catcacggct ttgctcgttt acgctgtccg caaatacaac ctgccgaagt ggtgtctgat
       61 tttgccgatc atcttcatcc ctggaatcgt tgtgccgatc tacctgtcgc aactgttggc
      121 catcccgttc ctgccgcttg tcgtcagcct ctgcatcggc caatcagtac cggcatttgt
      181 cggctacttt ttggtgcagg cgctggaaaa acgattttac aaaagtgcac tattaaaatc
      241 ttaagcgcaa ggaggaacaa caatgacagc aggcagaaat gaagccgatc tgaacggcat
      301 cacccaattg ggcaaccaac agactaaata tccgcaggac tacgatccga aagtcctcga
      361 aacttttccg aacaagcatc cggacaacga ttacattgtg aaattcaact gccccgaatt
      421 cacaagcttg tgcccgatga cgggacagcc cgattttgcg accatctaca tttcctacgt
      481 cccgggagag atcatggtcg aaagtaaatc gttgaagctt taccttttca gtttccgcaa
      541 ccacggcgac ttccacgagg attgcatgaa catcatcatg aaggatctga ttgcattgat
      601 ggcgccgaaa tacatcgagg tgtggggcaa attcacaccg agaggcggca tctcgatcga
      661 tccttactgc aactacggca aacctgacac aagatgggca gacgtggctt ggcaacgatt
      721 ggcgcagcac gatctttatc ctgagaaagt cgataaccgc tagatggaaa atatgaatgg
      781 gacaacgcaa aaacaagcct tggtcatctt ttcaggcggg caggattcca caacttgcct
      841 gatccaagcc atccaaaagt acggcgcggc aaacacagtg gccctttcct tcagctatgg
      901 ccaacgccat ggcatcgaac tggaacgggc ggcctggatc gctaacgatc tgggggtcga
      961 gcaccacatc ctggacgcga gcgtcatggg caaagtcacg acgaacgcat tgatggatga
     1021 gtcgcagaca atcaaggaag ccgatgctga ggactatccg aataccttcg tcgacggccg
     1081 gaatgctctg ttcctgttgc tggccggcat ctttgccaag tccaaagaca tccgcataat
     1141 tatcctaggc gtgtgcgaaa cggacttcag cggttatccg gactgccgcg atgtgttcgt
     1201 caaatcgatg aacgtgaccc tgaacttggc catggattac aatttcaacg tcgaaacgcc
     1261 gttgatgtac ctgaccaaag cgcagacatg ggagctggcg gatcagttgg gttgcctcgc
     1321 ctacatcgag gaacataccc atacttgtta tatgggtgtc cccggtggtt gcggcgaatg
     1381 cccttcctgc aaactccgca acgccggact ggcagaatat ttgaataaat aagaaaaaat
     1441 agccctctgg ctgttccgct gctgtggatc atccggaggg ctatttgttt tcaccaaagg
     1501 caactgtcca gtctctgggt caccatacat acagaagcaa acgtttgctt tcctgcctat
     1561 ccagcttact gtccttgtcc agcattccct tcctgatttt ccatatcctt attcaaagct
     1621 ttcatttcat cagccagcaa atcggcctta tcggtacgct cccaaggaag atccaattcg
     1681 ggcctgccga aatgaccata acaagcaagc tttctgtaga ttggcttccg cagatcgaaa
     1741 tcgcggatga ttgcggctgg gtgcaggtcg aagtgtttct tgattagttc ttcgatctgt
     1801 tcttcggcga ggctagccgt cccgaaggtt tcgactcgga cggaaacggg cctcgccact
     1861 ccgatggcat tgacgaaata tttcgtctga tcatccaaca gctccgccgg gataacggcc
     1921 ggaatcacca ttcgaagcat gtcctctctg atccgttcct gatcgaccga ttcgtcatgc
     1981 tgacaggcga tcacgaccgc ttcgatcctt ttgacatggc cgtccgcata ttccacggtc
     2041 acctgcgttt ttnnnnnnnn nncccctgat ttccgcagct cggcaacctt tttggccagc
     2101 ctatgagcca acatgatcgg cagcggcatc aactccggcg tttccttgca ggcatagccg
     2161 aacatcaatc cctgatcgcc tgctcccaat tggtccgctt catcgtccga tccgagccgc
     2221 ttttccaaag aggagccgac ccctgcagcg atatccggtg attgcctgtc caaaccgatc
     2281 aggacggaac aagccttccc atcgatacca aagtcgctgt ccgtgtagcc gacagcctga
     2341 atgatggacc gcacgatggc cggaatatcc accgtgctga cagtcgtgat ctggccgaaa
     2401 accgtgatca aaccagtact ggcggaaaca tcgcaagcca cccgcgcgaa cggatcctcc
     2461 ttcagaatgg catccaatat cccgtcggct atctgatcgc agagcttatc cggatgcccc
     2521 tccatgaccg attccgaggt gaaaaatgtt ctcttcatac tttatcgcct ctttttatta
     2581 aaaaatgaat gacacaacct tccaaaaact tgttttacaa acttcacgca tccttgattt
     2641 gctcttcgga ctccgaaggc atgaaggttg cgacaaggta accgaccccg aaaggacctt
     2701 cgtaggagag cacttccgct tcatatgccc tgccttcgaa cgctcccatc aggaacagga
     2761 tggagcgcag cccgcactcc gccgcccttt caaggaaccc ggcatccttt tccttaatgg
     2821 ctttgaccac taatttatca aacaaaggcc ccgccgaatg gtagccgtaa ggcccctccg
     2881 gcttcagcac gtgcgacaga tcggcagaag cgatgatggc agtgcgcaga tccgatcggt
     2941 cgtaagcttc cccataacgt tttccccatt cgtagtagtg ctcgtaggaa ggcaccgtca
     3001 ccaaaatctt ttccgtttcc aggttgggcg gcagttgtct gtcgagataa tgcatgggca
     3061 cttcaaaacc gtggccttca tgcggtttca caatacaaaa atttaacagg cagatttccg
     3121 aaaaatccac catgccttga tactggcata aatttttttg taaacgaaaa agtaaaatag
     3181 cctctgattc attttattga tcaagttggt gaatattggg ctataattag taaaactata
     3241 tgtca
//
LOCUS       DAOMUJ010000178         3242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8420_length_3242_cov_1.929087, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000178 DAOMUJ010000000
VERSION     DAOMUJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8420_length_3242_cov_1.929087"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLT73_05410"
     CDS             complement(<1..436)
                     /locus_tag="PLT73_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM19851.1"
                     /translation="MQNIEDREATILKYVPLVEKVVRHIGLSNSDYEKSDLVNIGVIG
                     LMDAIDKYDEDKKVPFENYAFIRIKGTIIDEIRRNGPVSRTGMNKLKDYRLAVESLQH
                     SLKRIPTEEEICQELQITSKQLGQVYDTASYLATQSLEKMIFN"
     gene            complement(497..2587)
                     /gene="flhA"
                     /locus_tag="PLT73_05415"
     CDS             complement(497..2587)
                     /gene="flhA"
                     /locus_tag="PLT73_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRM19852.1"
                     /translation="MAAQLTKKNRNASAFMDVGIATFVVLILAMIIIPLPAPLLDLLL
                     IVNLALSMTILLLTLFTKNVLEFSTFPTLLLLTTMFRLGLNISSTRLVLTEGDAGKVI
                     DTFANLVAGNNIIVGAVIFIIIFIIQMIVVTNGAGRVSEVSARFTLDAMPGKQMSIDS
                     DLNSGLINEQQAKQRRNDLERETQFFGAMDGASKFVKGDATAGIIITFINLIGGTLIY
                     SLQQGMPIMEALNKFGRLTIGDGLVSQIPSLLISIGSGILVTRSGNAKTFGTSLIPEL
                     MSSSKVLYLVSAILLVFALLPGFPTFTFSMMAILLGGSSYLIQQNETKAQFEKITEEQ
                     RILAGERMLQNQSNEETVTAHQVDPISIEIGYGLIAIADETKDDNLMNQIHSIRKQCA
                     TEIGILLKPIRIKDNLQLSMNQYVIKIKGNVYAQGELYMDRYLILSPGDTEFAFNGIA
                     TKEPSFGLDAMWITGDDREMADLQGYTVVDPLTVFVTHLKETIFQHSQELLGRQEVKQ
                     LLETLKETHNVVIDELIPDILRLGEVQKVLQNLLKENIPITDMVTVLETLADYGTTVK
                     DIELLTEYVRQALKQTIVKNYLDENAVLNVVTIHPNTEEMLGKNIQKSSSGSIPLIQG
                     NNVTKLFDSIKKIHNHLEAEGRPHVLLVSPKIRPSLKNLLTFNFPEIAVLSLNEVPNE
                     IAIESVGMVEDIAI"
     gene            complement(2608..>3242)
                     /locus_tag="PLT73_05420"
     CDS             complement(2608..>3242)
                     /locus_tag="PLT73_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRM19853.1"
                     /translation="KNILKMILIGYVCYISFEKSGYYIINSSAVGTEKLYFLILELVK
                     DISTNLAILLFLLGMVDFIYQKYDYKKNLNMTKQEIKDEYKESEGDPYIKSLRRQKYK
                     QFSKQMLKDVESATVIITNPTHLAIAVRYDKKRDQVPVVVAKGADYVAEKIREAASKN
                     NIPIMENKPVARAMYRSVEIGQPIPLDFYEAIAEMIALIYQIDESNKRKI"
BASE COUNT          862 a          731 c          622 g         1027 t
ORIGIN      
        1 cattaaagat cattttttcc aatgactgcg tagccagata agacgccgta tcatatactt
       61 ggcccaattg tttggaagtg atctgaagtt cttggcatat ttcctcttcg gtgggtattc
      121 tttttaaact gtgctggagc gactccactg ccagcctgta atcctttaat ttattcatcc
      181 ctgttcggga aaccggacca ttacgacgaa tctcatcgat tatggtccct ttgatccgaa
      241 taaatgcata attttcaaaa gggactttct tgtcttcatc gtatttatcg atggcatcca
      301 tgagtccgat gacaccgatg ttcaccaaat cgctcttctc gtaatcagaa ttactcaatc
      361 caatgtgtcg gaccactttt tcgactagag gcacgtactt caatatggtg gcttctctgt
      421 cctctatatt ttgcatatcc cgctcccatc ttcaggtgat tgaaaattca ttttgtatac
      481 tgaccctcga gtgacattaa atagcaatat cctcaaccat accgactgat tcgattgcga
      541 tttcgttcgg tacttcattc aaagataata cggctatttc cggaaaatta aatgtcaaca
      601 gattttttaa agagggtctt atttttggag acaccagcag gacatgcggt cttccctcag
      661 cttcaagatg gttatggatt ttctttatgc tgtcgaataa ttttgtgaca ttatttcctt
      721 gaatcaatgg aatcgatccc gatgaagatt tttggatatt tttaccgagc atttcctcag
      781 tgtttggatg gatcgtaacg acattcagca ctgcattttc atccaaataa tttttcacga
      841 tagtttgctt cagggcttga cggacgtatt ccgtcagtag ttcaatgtct tttaccgtgg
      901 tgccatagtc agccaacgtc tccaatacag taaccatgtc ggtgatcggt atattttcct
      961 tcagcaagtt ctgaagcact ttttgcactt cgcccaatct gaggatatcc ggtatcaact
     1021 catcgatgac aacgttatgc gtttctttca gcgtttccaa tagttgcttc acttcttgcc
     1081 tgccgagcag ttcctgacta tgctgaaaaa tggtttcttt aaggtgtgta acgaacacag
     1141 taagtgggtc taccaccgta tacccttgca aatctgccat ttcccgatca tccccagtta
     1201 tccacatggc atccagcccg aacgatggct cttttgttgc gataccatta aaagcaaact
     1261 ctgtatctcc tggcgacaag atcaagtagc gatccatata caactcaccc tgtgcgtaga
     1321 cgttcccttt aattttgatt acatattggt tcatactcag ttgcagatta tctttgatac
     1381 gaatcggctt caatagaatg ccaatttccg tggcacactg ttttcggatg ctatgtattt
     1441 ggttcatcag attatcgtct tttgtttcat cagctattgc gatcagaccg tagccgattt
     1501 caatggatat aggatccact tgatgggcag tgaccgtttc ctcgtttgac tggttttgaa
     1561 gcatccgctc tcctgccaga atgcgttgtt cttcggttat tttttcaaat tgtgctttcg
     1621 tctcattttg ctggataaga tagctcgacc cgcccaatag tatcgccatc attgaaaagg
     1681 tgaatgttgg aaaccctggc aatagtgcga acacgagtaa aatagcggaa accagataga
     1741 gtacctttga actggacatc aattcaggta tcagtgatgt accgaatgtt tttgcgtttc
     1801 cggaccttgt gaccaaaata cctgaaccga ttgaaatcaa taaggaaggg atctgactga
     1861 ccagaccatc tccgattgtc aatctcccga acttattcaa cgcttccatg atgggcatac
     1921 cttgttgcag agaataaata agcgttccac cgatcaagtt gatgaaggta ataatgatgc
     1981 ccgcagtagc atcgcccttg acgaatttac ttgcaccatc catcgcccca aaaaattgtg
     2041 tttcccgctc taaatcgttg cgtcgttgct ttgcttgctg ttcattgatc agaccggaat
     2101 tcaaatccga atcgatcgac atttgcttcc cgggcatcgc atccaatgtg aaacgtgcgg
     2161 aaacttccga tacccgaccc gcaccattcg tcacgacgat catttggatg atgaaaatga
     2221 tgataaaaat gacggcaccg actatgatat tatttccggc aaccaaattc gcgaatgtat
     2281 cgataacctt accagcatcg ccctcagtca gaaccaaccg cgtcgaagag atattcaacc
     2341 ccaacctgaa catggtcgtc agtaaaagga gcgtcggaaa agttgaaaat tccaggacgt
     2401 ttttagtgaa taacgtcaac aacaggatcg tcatggacaa ggctagattc acaatcaaca
     2461 gtaaatcaag aagtggtgca ggtaatggaa taatgatcat tgccaaaatg aggactacaa
     2521 acgttgcaat ccctacatcc ataaacgctg aagcattccg attttttttg gttagttgtg
     2581 cagccattac atcactcttt ctctcactta tattttcctc ttgttgcttt catcgatctg
     2641 atagatcaac gcaatcatct cagctatcgc ttcataaaag tccaatggta tcggctggcc
     2701 aatctcaact gatcggtaca tggcgcgtgc cactggtttg ttttccataa tcggaatatt
     2761 attttttgaa gctgcttccc tgattttttc tgccacatag tctgcaccct tggcgacgac
     2821 tacaggcacc tggtcgcgtt ttttatcata acgcacggca attgccaagt gcgttgggtt
     2881 tgtgatgatg acagtcgcag actccacatc cttcagcatt tgtttactga attgtttata
     2941 tttttgacgt cttaatgatt ttatgtaagg atcgccttct gattctttgt attcatcttt
     3001 tatttcttgt ttggtcatat ttaaattctt tttatagtca tatttttgat agatgaaatc
     3061 caccatccct aaaagaaaca acaagatggc aagattcgta ctgatatctt tgacaagttc
     3121 cagaatcaaa aaatagagtt tttcggttcc aactgcagac gagttgatga tgtagtaacc
     3181 cgatttttcg aacgatatgt aacagacata accaatcagg atcatcttaa gaatattttt
     3241 ga
//
LOCUS       DAOMUJ010000179         3241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8421_length_3241_cov_2.022913, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000179 DAOMUJ010000000
VERSION     DAOMUJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_3241_cov_2.022913"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLT73_05425"
     CDS             <1..703
                     /locus_tag="PLT73_05425"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRM19854.1"
                     /translation="QPRVSDKRYVKGEYLIKIDHHPNEDPYGDICLVETQASSSSEII
                     AKISFSTGDKLPMNDAAARLLYAGIVGDTGRFLYPATTAMTMKIASKLMQFDFSASDI
                     SQMMNTNSLKTANLSGFVLQSLTINDVGVASIILSQEILGRFGAVDSDTAPVVPLPGT
                     VEGVLCWGIFVEQPNGTYRCRLRSKGPIINEVAKRHGGGGHPLASGANAKDESEINDI
                     ILELEELAIEWQNTH"
     gene            complement(770..>1017)
                     /locus_tag="PLT73_05430"
     CDS             complement(770..>1017)
                     /locus_tag="PLT73_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRM19855.1"
                     /translation="LDCLNGDPTNFSHWDEVAQSWHFVDQIRKVWDDQEDCTDTLFAY
                     ESHSMGPKESDALLEQDGFHWTEFNWSQSYKEKQDNE"
     assembly_gap    1018..1039
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1040..2295)
                     /gene="zwf"
                     /locus_tag="PLT73_05435"
     CDS             complement(<1040..2295)
                     /gene="zwf"
                     /locus_tag="PLT73_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRM19856.1"
                     /translation="MITQNTALFTIFGATGDLAHRKLYPSLFRLYKKGFIKDNFAVIG
                     TARREWSDVHFREVVLDSIKDLIEDKEDAISFAAHFYYIAHNVNDAEHYIKLKDLSES
                     LDAKYSLSGNRIFYLAMSPEFFGTISEKLKEQKLLTQDGFNRLIIEKPFGRDFESADA
                     LNTKLRKSFYENQIFRIDHYLGKEMVQNISAVRFSNMIFESLWNNKYIDNVQITLSET
                     VGVEERGAYYDNNGALRDMVQNHILQIVALLVMEPPLSLQGEDVRSEKIKALRSLRMY
                     NTPEEVNRNFIRAQYAGNPAKALQAYREEPNVNPASSTETFVAGKILVDNFRWKGVPF
                     YIRTGKQMASKETYIHIQFKHVSMNIFPTEGVDEIAEPNVLTIHVSPLEGFSLRLNAK
                     RVGQGTEMKNIRMHHIYDEETQANSP"
     gene            2517..>3241
                     /locus_tag="PLT73_05440"
     CDS             2517..>3241
                     /locus_tag="PLT73_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM19857.1"
                     /translation="MVGHKIRRRKTRGVNVEFKDFQLKPFLLEAISKLNFKEPTDIQK
                     QIIPIIRTGKSVIGQSQTGTGKSHSFLLPLIDAIDPAKNEVQVVITSPSRELAEQLYQ
                     TATQLISNASEEIRIVSYVGGTDKKRQMSKLINNQPHIVIGTPGRILDLVNEQALKIH
                     TASKMVIDEADMTLDLGFLHDVDQIAGRLPEKLQMLVFSATIPEKLKGFLKKYVENPV
                     IVQLKPEHIIAPAIDNLLLSTKGR"
BASE COUNT          898 a          690 c          742 g          889 t
ORIGIN      
        1 tcaaccaaga gtcagcgata aacgttatgt taaaggggag tatttgatca aaatcgatca
       61 tcatccgaat gaagacccct atggagatat ttgccttgtg gagacccaag ccagcagcag
      121 cagtgaaatc atcgcaaaaa tttccttttc aactggggat aagctgccga tgaatgacgc
      181 agctgcgcgt ctgctgtatg ccggcattgt aggcgatacc ggcagattcc tttatccggc
      241 gactacggca atgaccatga aaatagcctc caaactgatg caatttgatt tttccgcgtc
      301 ggatatcagt caaatgatga atacaaattc tctgaaaaca gccaatttat caggtttcgt
      361 tctgcagagt ttaacaatca acgacgttgg ggtagccagc attatccttt cccaagaaat
      421 tcttgggaga tttggtgctg tcgacagtga caccgcaccg gttgttcctt tgccgggcac
      481 tgtagagggc gttttgtgtt gggggatttt tgtggagcaa ccgaacggga cctatcgttg
      541 ccgtttgcgc tcaaaaggtc cgatcatcaa tgaagtcgcc aaacgccatg gcggaggcgg
      601 tcatccgctt gcaagcggag caaatgcgaa agatgagtca gaaataaacg acatcatttt
      661 ggaattggaa gagttggcga tcgaatggca gaatacgcat tgatgaagct gacttgaatc
      721 gaaaaaggcc gggcagaagc gaaataacgc ttttgtccgg ccttcagttt tattcgttgt
      781 cctgtttttc tttataactt tgactccagt tgaattcagt ccagtggaag ccgtcctgtt
      841 caagtaaagc gtcactttct tttggaccca tcgagtggct ctcataagcg aaaagggtat
      901 ccgtacaatc ttcttggtca tcccaaactt tgcggatttg atcaacgaaa tgccaagatt
      961 gcgcgacttc atcccagtgg gaaaagtttg ttggatcgcc gttcaggcaa tccaatannn
     1021 nnnnnnnnnn nnnnnnnnnt cagggctgtt tgcctgcgtt tcttcatcgt agatgtgatg
     1081 catacgaata tttttcatct cagttccttg accgacacgc ttggcgttca ggcgtaagga
     1141 gaagccttcc aacggcgaaa catggatggt cagcacattc ggttcagcga tttcgtcgac
     1201 gccttctgta gggaagatgt tcatcgagac gtgtttgaac tggatatgga tgtatgtctc
     1261 tttagaagcc atttgtttac cggttcggat gtagaaggga acacctttcc aacggaagtt
     1321 atcgactaat attttgccgg ccacaaaggt ttcggttgat gaagcagggt tgacgtttgg
     1381 ttcctcccgg taggcttgca aagcttttgc aggatttccg gcatactggg ccctgataaa
     1441 gttgcggttg acctcttcgg gtgtgttgta catacgaaga gaacgaagag ctttaatttt
     1501 ctcggaacgc acatcctcgc cttgcaagga aagcggcggt tccataacca acaaagcaac
     1561 aatctgaagg atatgatttt gcaccatatc gcgcaaagcg ccattgttgt cgtaataagc
     1621 gccgcgttct tccacgccga cagtctcgga gagggtgatc tggacattgt cgatgtactt
     1681 gttgttccac agcgattcga aaatcatatt ggagaaacgg accgcagaga tgttctgcac
     1741 catctcttta cccaaataat gatcgatacg gaagatttga ttttcataaa acgacttgcg
     1801 caacttcgtg ttcaatgcat ccgcagattc gaaatcccgc ccgaaaggtt tctctatgat
     1861 cagacggttg aacccgtctt gcgtaagtaa cttctgttct ttcagttttt cggaaatggt
     1921 gccgaaaaac tccggagaca tcgccaagta aaaaatgcgg ttccctgaca gtgaatactt
     1981 ggcatccaaa gattcggaca agtccttcaa cttgatgtag tgttccgcgt cattgacgtt
     2041 gtgtgcgata taatagaaat gagcagcgaa agaaattgca tcttccttat cctcgatcaa
     2101 atctttgatg gaatccaaga ccacttcacg gaaatgtaca tcagaccatt ctcgtctcgc
     2161 tgttccgata acggcaaaat tatccttgat gaatcctttt ttgtataagc gaaataatga
     2221 cggatacagt ttgcggtgag ccaaatcacc agtcgctccg aaaatagtga acaaagcagt
     2281 attttgggta atcattacat gccccccttt agtattatga ttagatttta ttcattatat
     2341 cattatacta ttatcattta gccttggcaa cgaaagaaag tgtctgtttt catgagtgtg
     2401 gtatgttcta gagagtgttt tcgcataagt ttgtaaatat gttgccaata aatttatggg
     2461 tcctcctttc acattcttat ttgaaattaa ggtttaaacc ttctggatgt gttataatgg
     2521 tagggcataa aatacgcaga agaaaaacaa gaggtgtaaa cgtggaattt aaagactttc
     2581 aattgaagcc atttttgctg gaggcaatca gcaaattgaa tttcaaagaa ccgacagata
     2641 tccaaaaaca aattatcccg atcattcgga ctggtaagag tgtcattggc caatcgcaaa
     2701 caggaacagg caaaagccac agtttcttgc tgcctttgat cgatgccatc gatccagcaa
     2761 aaaatgaagt tcaagttgtc atcacttcac ctagtcgtga attagctgaa caattgtacc
     2821 aaacggccac ccaactcatt tcgaatgctt cagaggaaat ccgtatcgtc agttacgtag
     2881 gcggtacaga caagaaacgt caaatgtcga aacttatcaa caatcaaccg cacatcgtta
     2941 tcggaactcc gggcaggatc ctcgatctgg tcaacgagca agcgttgaag atccatactg
     3001 catccaaaat ggtcattgat gaagcggata tgacactgga tttgggcttc ctgcatgatg
     3061 tcgatcagat tgccggacgt ttgccggaga aactgcaaat gctggttttc tctgctacga
     3121 ttccagagaa actgaagggt ttcctgaaga aatacgtcga aaaccctgtt attgttcagt
     3181 tgaaaccgga acacatcatt gcgccagcaa tcgataatct gttgctgtcg actaaagggc
     3241 g
//
LOCUS       DAOMUJ010000180         3241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8422_length_3241_cov_1.738544, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000180 DAOMUJ010000000
VERSION     DAOMUJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8422_length_3241_cov_1.738544"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>1252)
                     /locus_tag="PLT73_05445"
     CDS             complement(85..>1252)
                     /locus_tag="PLT73_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRM19858.1"
                     /translation="DITALQNEKLELNEQIATYHEILKQEKTLYAVMKNELATVKKQY
                     ANPRRTTIQHEIEEIKIDTEVLVPIEEVIVVITKEGYYKRTSLRSFGASTIEEIGLRE
                     GDKPLFVGKASTLDAIVMFTNKGDYLHFPVHELPELKWKDTGHHISQNIPFASEEKII
                     SVEMIPNNGEPTGTLLFVSKEGYIKQTLMSDFRTFRGYRNKKAVAMKMKSETDTIVSI
                     QRLEDKQNYEVLLVTHFGFSLRYAVSEVGIIGTRASGVKSINLKEGDFVVGGAAFKEG
                     DDEFPILVLTQRGNIKKFKSGEVPILGRAKRGLMLLKELKAKPHRIIFMDYNLSIPNH
                     YAIRTSNGKTIMVESKETPFSPRYSNGSFAINEKTDGEVSDIEKEFDFSQIRIS"
     assembly_gap    1253..1262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1263..2518)
                     /locus_tag="PLT73_05450"
     CDS             complement(<1263..2518)
                     /locus_tag="PLT73_05450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HRM19859.1"
                     /translation="MLENVMGDRFGRYSKYIIQDRALPDIRDGLKPVQRRILYAMDVA
                     GNTSEKGFRKSAKTVGNVIGNYHPHGDSSVYEAMVRLSQDWKMREVLVEMHGNNGSMD
                     GDSAAAMRYTEARLSKISAELLRDIDKETVDFVLNFDDTDQEPTVLPARYPNLLVNGA
                     TGISAGYATDIPPHNLGEVIDATLHLIDHPDAELKALMKYVKGPDFPTGAIIQGLEGL
                     QDAYRTGKGKVVVRSRTSIESIRGGKQQIVISEIPYDVNKATLVKRMDEIRINRKIEG
                     IAEVRDESDRSGLQIVVELKKEANAEGILNYLLKNTDLQVNYNFNMVAIDERRPMQVG
                     LHQILKAYIRHQKIVITRRTNYNLKKAETRLHIVDALIRVISILDEVIQLIRDSKDKK
                     DSKQNLMRQYAFSEAQAEAIVSLQLYR"
     assembly_gap    2621..2673
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2674..>3241)
                     /locus_tag="PLT73_05455"
     CDS             complement(<2674..>3241)
                     /locus_tag="PLT73_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM19860.1"
                     /translation="GKVINTERASMQDILKNEEINTIIYTVGAGVGADFDIEDCNYDK
                     IIIMTDADTDGAHIQILLLTFFYRYMKPLLEAGKIYLAQPPLYKISKGFGKNEKIEYA
                     WTEKELQEKIDIIGKGYILQRYKGLGEMNADQLWDTTMDPETRTLIRVLIDDKAQAER
                     RVTTLMGNKVEPRRKWIEKHVEFSMADDK"
BASE COUNT          732 a          794 c          656 g          996 t
ORIGIN      
        1 tttgacctaa aatctgtttc ataaaaaaac ggaaactgta ctattacaca gtttccgttt
       61 tgatattttg tttataacag aaaattagga aattctaatt tgtgaaaaat caaactcttt
      121 ttcgatatcc gatacttcgc catcggtctt ctcgttgatt gcgaagctgc cgttggaata
      181 tctcggactg aatggcgttt ctttgctttc caccataata gtcttgccgt tgctcgttcg
      241 gattgcataa tgattaggga tggacagatt gtaatccata aaaatgatcc ggtgtggctt
      301 ggccttcagc tccttcagaa gcatcaaccc tcgttttgct cgacccaaaa tcggaacttc
      361 tccggatttg aattttttga tgttccctct ttgtgtaagc acaagaatcg ggaactcatc
      421 atcaccttct ttaaatgcgg ctccccctac tacaaaatct ccttctttca ggttaatgga
      481 cttaactccg ctggcgcgcg ttccgatgat gcccacctcg ctgacggcgt atcgcaaact
      541 aaatccgaag tgcgtcacca acagaacctc gtaattttgc ttatcctcca gacgttggat
      601 tgaaacgatc gtatctgtct cgctcttcat cttcatggcg actgcctttt tattcctgta
      661 accacggaaa gtccggaaat cgctcatcag tgtctgtttg atgtaccctt cttttgatac
      721 aaaaagaagg gtaccggttg gttcaccgtt attcggaatc atttccacac tgatgatttt
      781 ctcctccgaa gcgaagggaa tgttttgcga gatatgatgg cccgtatctt tccatttcaa
      841 ctccggcaac tcatgtaccg ggaaatgaag atagtccccc ttgttggtga acatcacgat
      901 ggcatccagg gtcgaagctt tcccgacaaa tagcggctta tctccttcgc gcaagccgat
      961 ttcttcgatt gtcgacgctc caaaagaacg caggctggta cgcttgtaat agccttcttt
     1021 agtgattacg acaatgactt cttctatcgg caccaacact tccgtatcga ttttgatttc
     1081 ctcgatctca tgctggattg tagtcctgcg agggttagcg tattgctttt tgaccgtggc
     1141 aagctcattt ttcatcacag cgtacaatgt tttttcttgc ttcagaattt catggtacgt
     1201 cgcaatctgc tcattcagtt ccaacttttc gttttgcagg gcagtgatat ccnnnnnnnn
     1261 nncggtacag ttgtaacgaa acgatagctt ccgcttgcgc ttccgaaaaa gcatattgcc
     1321 tcatcagatt ctgtttcgag tcctttttgt ctttgctgtc acggatcaat tgaatgactt
     1381 catcaaggat cgaaatcact ctgattaatg catccacaat gtgcaggcgc gtctctgcct
     1441 tcttcaggtt ataatttgtc ctgcgggtga tgacgatttt ctgatgacgg atataagctt
     1501 tcagtatttg gtgcaacccg acctgcatcg gacgacgttc atcgattgcg accatattga
     1561 aattatagtt cacttgcaga tccgtatttt tgagcagata attgagaatc ccctctgcat
     1621 ttgcctcttt tttcagttca acgacaatct ggagtcccga gcgatccgat tcatcccgca
     1681 cttcagcgat tccttcgatt ttacggttaa tgcggatttc atccatcctc ttcacaagcg
     1741 tcgctttatt gacgtcataa ggaatttccg aaatgacgat ttgctgtttg ccgccacgaa
     1801 tgctctctat ggacgtgcgc gaacggacaa caactttacc ttttccggtt ctgtatgcat
     1861 cctgcaagcc ttccaacccc tgaatgattg ctccggtagg gaagtcaggt cctttaacat
     1921 atttcataag cgctttgagc tccgcatcag gatgatcgat caggtgcaaa gttgcatcga
     1981 tgacttcacc taaattatgc ggcgggatat cggttgcata ccctgcggaa atgccggtag
     2041 cgccgttcac caagagattc gggtatctgg caggcaacac ggtaggttcc tgatcggtat
     2101 cgtcgaagtt cagtacaaag tctactgttt ctttatcgat atcgcgtagc aattctgcag
     2161 aaattttcga aagacgtgct tcggtatacc gcatcgctgc agctgaatcg ccatccatgc
     2221 ttccgttatt gccgtgcatt tccaccaata cttctcgcat cttccaatcc tgactcaaac
     2281 ggaccatcgc ttcatagaca ctggaatcgc catggggatg gtaattaccg atgacattcc
     2341 cgacagtttt agctgatttt cggaagcctt tttccgaagt gttcccggca acatccatcg
     2401 cataaagaat ccgtctttgg acaggtttca gtccatcgcg gatatcaggc aaagcccgat
     2461 cttggatgat gtacttcgaa tagcgtccga aacgatctcc cattacgttt tcaagcatca
     2521 gctctttaat ttccagatta tccactattg ttcaccactt tcaaaatcta acgaaatctg
     2581 ctccatgtcg tgagcggtct gttgaccaga agacgctttt nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttatca tcggccatcg aaaattcgac
     2701 atgcttctcg atccattttc ttcgcggttc gactttgttc cccatcagtg tcgtgacacg
     2761 gcgctcggct tgggccttgt cgtcgatgag aacccggatc aacgttctgg tttctgggtc
     2821 cattgtagta tcccatagct gatccgcgtt catttctccc agtcctttat agcgttgcaa
     2881 gatgtagccc ttaccgatga tgtcgatctt ttcctgcagc tccttttcgg tccatgcgta
     2941 ttctattttt tcgtttttgc cgaaaccctt ggatatttta tagaggggcg gttgcgccag
     3001 ataaattttc ccggcttcca gcagtggttt catgtatcga tagaagaaag tcagcaacag
     3061 gatctgaatg tgtgcaccat ccgtgtccgc atccgtcata atgataattt tgtcataatt
     3121 gcaatcctcg atatcaaaat ccgccccgac gcccgcgcct actgtgtaga tgatcgtgtt
     3181 gatttcctcg ttcttcagta tatcttgcat gctcgcgcgt tcagtattga tgactttccc
     3241 a
//
LOCUS       DAOMUJ010000181         3225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8477_length_3225_cov_1.808202, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000181 DAOMUJ010000000
VERSION     DAOMUJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8477_length_3225_cov_1.808202"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..556)
                     /gene="cbpB"
                     /locus_tag="PLT73_05460"
     CDS             complement(56..556)
                     /gene="cbpB"
                     /locus_tag="PLT73_05460"
                     /inference="COORDINATES: protein motif:HMM:NF041630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HRM19861.1"
                     /translation="MISSEVAALLLDNDACGDLMIHGENVANVHCSNNLNHAFLVLSQ
                     VKYSVIPVLDSKSRIKGLISIPMIIKAITEIDAIRYDKMETLKVEDVMDRNVQTIRPW
                     TDLEDILNYLIDHNFLCVTEDDGKFIGIITRKEILTRVNHLVHGIHRIYKLEKIEEKE
                     EVASKR"
     gene            complement(624..1142)
                     /locus_tag="PLT73_05465"
     CDS             complement(624..1142)
                     /locus_tag="PLT73_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM19862.1"
                     /translation="MIKILAVSDSHGNKDILNELALRYSNQVDHFVHCGDSELSSSDL
                     IWGIMSTVMGNCDYDYQMNDEYRFQAGSKNVLVVHGHQHSVRSSIAGLKREAEQANVS
                     LVFYGHTHIAKAEQEEGILFINPGSISQPRGTLMEKTYCIVTLDDQTATVTYYNDHHQ
                     EVVDLRKQFQML"
     gene            complement(1143..1766)
                     /locus_tag="PLT73_05470"
     CDS             complement(1143..1766)
                     /locus_tag="PLT73_05470"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HRM19863.1"
                     /translation="MNAIEKKKIVIATKNPGKARDFAVLFEPKGYEVVTLLDYPEIED
                     VEETGYTFEANARKKAETIAELLGMTVLADDSGICIDALDGQPGVFSARFAGGMKSDA
                     ANNAKVLAMLGSQENPSRKAHFHCTLVLAHPDRESLVVEGDIEGEIAEFPSGDYGFGY
                     DPLFYLPHLGKTMAELSDAEKNQISHRAIALKNLEKNWAAWLEKEAD"
     gene            complement(1782..2606)
                     /gene="racE"
                     /locus_tag="PLT73_05475"
     CDS             complement(1782..2606)
                     /gene="racE"
                     /locus_tag="PLT73_05475"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM19864.1"
                     /translation="MSKKAIGFIDSGVGGLTVVKQAMKQLPNESIYYLGDSARCPYGP
                     RPKEEVIQYTWEMTQFLLKKDIKMLVIACNTATAAALDVIRERIDIPVIGVINPGSRA
                     AIKHSKNDRIAVIGTKGTINSDVYKQTIKDKDKGISVISMSCPKFVPLVESNQYNGSI
                     AKKVVAETLKPLLKESVDTLILGCTHYPLLTPLIQNVMGPCVTLIDSGAETVSEVSTL
                     LDYFRLAESSHNKQASEYRFYTTGSPKLFSDIAENWLGQSNFTIEKVDLLNLIEND"
     gene            2869..3174
                     /locus_tag="PLT73_05480"
     CDS             2869..3174
                     /locus_tag="PLT73_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19865.1"
                     /translation="MSYQSIVTVVDKGLVDRVFESARCEESYCGTVISGRGSGMHENK
                     KLFNLALEPEKEILLVLTQEQHSEKIINQIEEAVNITAPGNGILFGINLKRVHGIAE"
BASE COUNT          810 a          759 c          689 g          967 t
ORIGIN      
        1 ggaagtgaag cactttttga tttattgggt taggcttgaa gcagaaatcc gcatgctatc
       61 ttttggaggc cacttcttcc ttttcctcga ttttctctaa tttgtagatt cggtggatgc
      121 catgcaccag atgattcact cttgtcagta tctctttgcg cgtgatgatg ccgataaatt
      181 tgccgtcatc ttcggtaacg cacaagaaat tgtgatcgat cagataattc aggatatcct
      241 ccaggtctgt ccatgggcgg atggtctgta cattcctgtc catcacatct tccactttga
      301 gtgtttccat tttgtcatag cggatcgcat cgatttccgt gatggctttg atgatcatcg
      361 gtatggaaat cagtcctttg atgcgggact tcgaatccaa aacgggaatg acggaatact
      421 tcacttggga caacacgaga aacgcatggt tcaggttatt cgaacaatgt acattggcga
      481 cattttcgcc gtggatcatc agatctccgc aggcatcatt gtccagcaat aacgcggcta
      541 cttcgctact aatcattaat ttttcctcct aaaaggtatc catccggaat gtgtgcagcc
      601 ggatgcacct tctttgcatg tgatcaaagc atttgaaatt gttttctcag atcgaccact
      661 tcctgatggt gatcattgta ataggtaaca gtagccgttt ggtcatccaa cgtgacgata
      721 caataggtct tttccatcag cgtgccacgt ggttggctga tgctaccggg atttatgaaa
      781 aggatgccct cctcctgttc cgctttggcg atatgagtat gcccataaaa caccagcgaa
      841 acattcgcct gctcagcttc tcgtttcaga ccggcaatcg agctccttac ggaatgctga
      901 tgcccgtgga caaccagtac atttttgctg ccggcctgga aacggtattc gtcgttcatc
      961 tgataatcat aatcgcaatt tcccatcaca gtggacatga tcccccaaat cagatcggaa
     1021 ctgcttaatt cagaatcacc gcagtgtacg aaatgatcaa cctgatttga atagcgtaaa
     1081 gccagttcgt taagaatatc tttattacca tggctgtcac tgactgcaag tatctttatc
     1141 atctaatccg cttccttttc caaccatgct gcccaatttt tctcaagatt tttcagggca
     1201 atcgcacggt ggctgatctg atttttttct gcatcgctca actctgccat cgtctttccc
     1261 aagtgtggca aataaaataa cggatcatat ccgaaaccat agtctccgct cgggaattcc
     1321 gctatttcac cctcgatatc cccttcgaca accagacttt cccgatccgg atgggccaac
     1381 actaaggtac aatggaaatg cgccttacgg gatgggtttt cctgggaccc gagcatcgcc
     1441 agcaccttgg cattattggc ggcatcactt ttcattccgc cagcaaatcg ggctgaaaac
     1501 acacctggtt ggccatccaa ggcatcgatg cagatgccgg aatcgtccgc taagaccgtc
     1561 atgcctaata attcagcgat tgtctcagct ttcttccgtg cattagcttc gaatgtatag
     1621 cccgtttcct caacatcttc aatctccgga tagtccaaca aagttacgac ttcatagcct
     1681 tttggttcaa ataggacggc gaaatctctg gcttttccag gatttttggt tgctattacg
     1741 attttctttt tttcaattgc gttcatcgta tccctcattt ctcagtcatt ctcgatcaaa
     1801 tttagcaaat caaccttttc aatcgtaaaa ttgctctgtc cgagccaatt ttctgcaata
     1861 tccgaaaaca gtttaggcga acccgtcgta taaaatcggt attcggatgc ttgcttgttg
     1921 tgagaactct ccgccaatcg gaaataatcc aagagtgtgc tcacctcaga taccgtttca
     1981 gccccggaat cgataagagt tacacaaggc cccatcacat tttggataag cggtgtcaag
     2041 agcggataat gcgtgcaccc cagaatgaga gtatcaacag attctttcag taaaggtttc
     2101 agcgtttccg caaccacttt tttggcgatt gatccgttgt attggttact ctccactagc
     2161 ggcacaaatt ttgggcagct catgctgatg actgaaatac ccttgtcttt gtccttgatg
     2221 gtttgtttat aaacgtcgct gttgatggtg ccttttgtac cgattacggc aatgcgatca
     2281 ttttttgaat gtttgattgc cgctcggcta ccgggattga taacgccgat caccggaatg
     2341 tctatgcgct cgcggatgac atccaaggcg gctgcggtcg ctgtgttgca agcaatcacc
     2401 agcattttga tatctttctt cagcagaaac tgtgtcattt cccatgtgta ttgaatcact
     2461 tcttctttgg gccttggtcc ataggggcat ctggcggaat cccccagata ataaatggat
     2521 tcattgggaa gctgtttcat tgcttgtttc acgacggtca agccgccgac gccagagtca
     2581 ataaaaccga ttgctttttt actcatgcag taacttcctt tatttgtttt cgtatctcta
     2641 ttttacctta agataaagga tttttgtaaa aatgaggcaa atttcattct gatttgcttc
     2701 acttttgtgg tttagactag attgctttct ccaatagggc tgccgattat tggcagtccg
     2761 aaattatgta tgctacaata gtctcaattt agggacggta ttttttgtcc tccagaagct
     2821 tccgatacta cgatggatgc aaaccaataa aaaggagtgg atattcaaat gagttatcaa
     2881 tcgattgtca cagtagtcga taagggattg gtcgaccgtg tcttcgagag tgcccgctgc
     2941 gaagaatcct actgcggtac cgtcatctca ggccgcggat ccgggatgca cgagaacaaa
     3001 aagttgttca atctggcgct ggaaccggaa aaggaaattc tgctggtttt gacccaagaa
     3061 cagcattccg aaaaaatcat caatcaaatt gaggaagccg taaacattac cgcacccggt
     3121 aatggcatcc tatttggaat caatttgaaa agagttcatg gcattgctga atagtaagaa
     3181 aagctgaacg ggttcgttca gccctgatta aggaaacact gtgac
//
LOCUS       DAOMUJ010000182         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8495_length_3221_cov_2.107707, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000182 DAOMUJ010000000
VERSION     DAOMUJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_3221_cov_2.107707"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="pta"
                     /locus_tag="PLT73_05485"
     CDS             <1..640
                     /gene="pta"
                     /locus_tag="PLT73_05485"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRM19866.1"
                     /translation="HMGLADGLVSGAVHSTGDTVRPALQIIKTKPGVSRTSGAFIMIR
                     GTERFLFADCAININPGAQELAEIAVESAKTAEMFEIQPNVALMSFSTKGSAASPEVD
                     KVVEATRIAKELAPQYNIDGELQFDAAFSEVVAKQKAPGSTVAGQAKVFIFPEIQSGN
                     IGYKIAQRFGGFEAVGPILQGLNKPISDLSRGCNEEDVYKTAIITANQTLLD"
     gene            795..1277
                     /gene="tsaE"
                     /locus_tag="PLT73_05490"
     CDS             795..1277
                     /gene="tsaE"
                     /locus_tag="PLT73_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRM19867.1"
                     /translation="MFTLTAKNEEETKWIAAQLAQLLEPKDVILLEGDLGAGKTTFTK
                     GLAKGLGIDTVIKSPTYTLIREYTKGRLPLYHMDVYRLEDVGGDDMGLEEYLYGNGVS
                     VIEWATFIEEELPDAYLTIRLVPTGEFYENREITFIPTGERYDALVETLQGILKKEGQ
                     "
     gene            1279..1809
                     /locus_tag="PLT73_05495"
     CDS             1279..1809
                     /locus_tag="PLT73_05495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRM19868.1"
                     /translation="MRKREKVEIMIREAIPADAKDIIAFSKKTGAETPYLAYGAEGLE
                     LSEAFEELYLEDLMEKDNNVMLIATINNKQLIGLASVGATDKPKLRHVGEVGITVEKE
                     YWGFGIGSVLMEEIETWAIESGVIRRLELTVHGENERAIHLYEKMGYQREAVMPRAML
                     IDGEFTDGVMMSLMID"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2474..>3221
                     /locus_tag="PLT73_05500"
     CDS             2474..>3221
                     /locus_tag="PLT73_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRM19869.1"
                     /translation="MKQKKSMKWALSLVGATALLAGCGAAGSTTENTESDKLQVVTTF
                     YPMYDFTKQVAQDDAEVSMLLEAGMEVHSFEPSSQMIAEIQDADVFIYNSPEMETWVP
                     DVLASIDTSDMVVICASDAITLLEYEGEAHAHDHESEEEGANAGHSHTVDPHVWLDPV
                     LAQTEVSTIAEGLAEADPDNAEDYLENAGIYNGKLNELDEAYRAAFEGTENRTFVTQH
                     AAFAYLAARYDLNQISVTGLNAEVEPSAAAL"
BASE COUNT          984 a          614 c          822 g          791 t
ORIGIN      
        1 acacatgggt ttggcagatg gcttagtgag cggtgccgtt cactctacag gtgatactgt
       61 ccgtccagca cttcaaatca tcaaaacaaa accaggcgta agccgcacaa gtggtgcttt
      121 catcatgatc cgtggtactg aaagattcct tttcgctgac tgtgcaatca acatcaatcc
      181 tggcgctcaa gaattggctg aaatcgctgt tgaaagtgct aagacagctg aaatgttcga
      241 aatccaacca aatgttgctt tgatgagctt ctcaacaaaa ggttcagcgg catcaccaga
      301 agttgacaaa gtcgtggaag ctacaagaat cgcaaaagaa ttggctcctc aatacaacat
      361 tgacggtgaa ctgcaattcg atgcagcttt ctctgaagta gtcgctaaac aaaaagcacc
      421 tggctcaact gtagccggtc aagccaaagt cttcatattc cctgaaatcc aatcaggaaa
      481 tatcggctac aaaattgcac aacgtttcgg tggtttcgaa gcagtaggcc caatcctgca
      541 aggtttgaac aaaccgatct ccgatttgtc gcgcggatgt aacgaagaag atgtctataa
      601 aacagccatc atcacagcaa accaaacatt acttgattaa taagagaatg gaaagcatgg
      661 atcaaatccg gctttccatt tttttttgca agccaaattg gactatgctc agaaaaaagg
      721 tgcattccgg agtgaatatt tctataataa ggaatggaac aaaataaatt ggtgaagatg
      781 ggggcagaaa cagaatgttc accttaacag caaagaatga agaagaaaca aagtggatag
      841 ctgcgcaatt ggcgcaacta ttggaaccga aagacgtcat actccttgag ggagatctag
      901 gtgccgggaa aacaaccttt acaaaagggt tggcgaaggg cttaggtatt gatactgtca
      961 taaagagccc cacgtatact ttgatcagag agtacacgaa aggcaggttg ccgctgtacc
     1021 atatggatgt ttatcgtctg gaggatgtcg gaggcgatga tatgggcttg gaggaatatc
     1081 tgtatgggaa tggggtttcg gtgattgaat gggctacctt catcgaagag gaattgccgg
     1141 atgcatattt gacgatccga ttggtcccta ccggggaatt ttatgaaaac cgggaaatca
     1201 catttatccc gaccggcgaa cgctatgatg ctttggtcga aacactacag ggaatactca
     1261 agaaagaagg acaataatat gcgcaagaga gaaaaagtcg aaatcatgat ccgggaagcc
     1321 attccggccg atgcaaaaga catcatcgct ttcagcaaaa agactggcgc tgagacaccg
     1381 tatttggcat atggagctga agggttggaa ttgtccgaag ctttcgagga actttatctg
     1441 gaagacttga tggagaaaga caacaatgtc atgctgatcg cgacgataaa taacaagcaa
     1501 ttgattggtt tggcgtctgt cggggcaacg gataaaccca aacttagaca tgtcggtgaa
     1561 gtcggcatta cggtcgagaa ggagtattgg ggattcggaa tcggatcggt ccttatggaa
     1621 gaaatcgaga cttgggcaat cgagagcggc gtgatccgcc gcttggagct gacagtccat
     1681 ggcgaaaacg aacgcgccat ccatctttat gaaaaaatgg ggtaccaacg agaagcggtg
     1741 atgccgcgtg ccatgttgat tgacggggaa tttaccgatg gcgtcatgat gagcctgatg
     1801 atcgattaat atacaaaagc agcgtgacgg tttaggtgnn nnnnnnnnaa atcgtcattt
     1861 tctgtttgac ttgataacgc ttacatgtta ttataactat acaaagagag acatactaag
     1921 taagactaag aaacaaaaaa gagtatgcca ctctttcagg gaaatccttc aaaaagattt
     1981 tccggttaac agcttttcag tcaagctgtt aatgcaaaat cttagatggt tcggaatttg
     2041 tgtcctagat aattttctaa gccatgaaaa cttgattcat tctaagatca ataaacagta
     2101 gatttcattc aagcaactgg aagcgaaagc ttagatgact tgcctgaaaa ccaagcgtaa
     2161 ccagtattga aaagttcgta gatcattaag attgaacaat caatgaagcg aggatgagac
     2221 tactgattta ggaggaagtg ttccgaaagt tggagcactt cctttttttt gcgttgtagt
     2281 aaggacagga gtgggttgct ttcgataagt ataaaaattt atacatatga aggaataaat
     2341 tatttgatac aaaatgcttg catctgcata atctttcagt tatcctttaa aacgtaatcg
     2401 ttacgatttg gtgagtgaga atcattagcc gatttttgga tagaccatag aaagaaaacg
     2461 gaggaagagc aaaatgaaac aaaaaaagag catgaagtgg gctttgtcgc tagtaggggc
     2521 aactgcacta ttggctggtt gcggagcagc cgggagcaca acagaaaata cggagtcgga
     2581 caagctgcag gttgtcacga ccttttatcc gatgtacgat tttacgaaac aggttgcgca
     2641 ggacgatgcc gaggtgagca tgcttttgga agccggaatg gaggtgcatt cctttgaacc
     2701 ttcgagccag atgatcgccg aaatccaaga tgccgatgtt ttcatctaca acagtcccga
     2761 gatggagaca tgggtgccgg atgtgctggc gtccatcgat acttcggata tggtggtcat
     2821 ttgtgcgagc gacgcaatca cgctgttgga gtatgaaggc gaggctcacg cgcacgatca
     2881 tgagtctgag gaggaagggg cgaatgctgg tcattcgcat actgtcgatc cgcatgtttg
     2941 gttggaccct gtgcttgcgc agacagaggt atcgacgatt gcggaaggat tagctgaagc
     3001 ggatccagac aatgctgagg attatctgga aaatgccgga atttataacg ggaaactgaa
     3061 tgaattggat gaagcctacc gagctgcatt tgaaggaaca gaaaaccgta ctttcgtgac
     3121 gcagcatgcg gctttcgctt atttggcggc ccgctatgat cttaatcaga tttccgtaac
     3181 gggtctgaat gctgaggttg aaccgagtgc agccgctttg g
//
LOCUS       DAOMUJ010000183         3198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8577_length_3198_cov_1.797964, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000183 DAOMUJ010000000
VERSION     DAOMUJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_3198_cov_1.797964"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1556
                     /locus_tag="PLT73_05505"
     CDS             321..1556
                     /locus_tag="PLT73_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016117108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM19870.1"
                     /translation="MLKSFSLQERSWIMYDWANSAYSVIISSVILPLFYKSITSGEGI
                     APNLADSYWGYATSAATLAIAVFAPILGTIGDYPKWKMRLFKSFFLIGVVATAALSFT
                     DDWRLLLAFYMLTTIGFSGANIFYDAFLVDVATEDRMDRVSTYGFAMGYIGGSTIPFV
                     LSILLIMFGEKIGIPRTTAIKASFLFTAFWWIVFTIPMLRNVKQRYFLPPTEHIIHDS
                     FARLNKTLHQIRSHKKMFLFLIAYFFYIDGLNTIIHMAAVFGDSIGVVSDVLMIAVLV
                     IPILSFPFTILYGALAKRFGSKKMILVGICIYLLVCLLAFRMTSAVEFWILAILVATS
                     QGGIQALSRSYFAKLVPKENANEYFGFYNILGKFAAIIGPALYALTSQLTGDSRNGLA
                     SISLLFIIGGVLMLRTEGN"
     gene            complement(1623..>2883)
                     /locus_tag="PLT73_05510"
     CDS             complement(1623..>2883)
                     /locus_tag="PLT73_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM19871.1"
                     /translation="RGNGAVVKQVDGTTLHIRRGSPEYFFGEGLISRDTVADWLQAEE
                     EEGHRVLGVSYDDGSGARFGGFVSFVDRIKDSTIETVAAAKQMNIAITVITGDALKVA
                     EAVGREAGLVTESAEVTEAAAFLTLPLAERKKRLSSIRVFARTTPEQKLELIQLLKEQ
                     FTVGYLGEGINDAPALKAAHVSMVVQSAADVARETADIVLLQNDLRVIVEGIRFGRET
                     HANTMKYIRATLISNFGNFYAVAIGSLFISFLPMLPKQLLLLNLLSDFPMMAIAFDRV
                     SAQEVERPQRYDLHSLYIIFVTMGLVSTVFDFICFGLFYRISPAVLQTNWFIASVLTE
                     ILLMLSIRSLAPITKAGWPAPSIVILSVIAMALAIGLPMIPAAAAFFEFQPPTMAHLG
                     IILGIAVSYLVVTELVKRPLSGFVSKR"
     assembly_gap    2884..2905
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2906..>3198)
                     /locus_tag="PLT73_05515"
     CDS             complement(<2906..>3198)
                     /locus_tag="PLT73_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19872.1"
                     /translation="EFAKRDVIVKRLSAVQDLGSVNLLCTDKTGTITENHLVFSNVYP
                     MAESPYDPLVLARLAAINLHERIPEPFDHASDEALTQAQRQIVERYSLAQEEA"
BASE COUNT          716 a          871 c          793 g          796 t
ORIGIN      
        1 aacggaacaa ggtgccgaac ccgctgtcca catccggatc agcggcattg tcgtattcca
       61 cgacgtagaa atcccggtat tccctcaatt cgaacggata ctccgacagc aggaccgtgt
      121 attccgggcg ctcatatttg gccatgactt cccttccccc attctgttct ctatagctga
      181 attttaccat gattttcttt ttttccgaaa agataggctg cttgactggc ttggggcttg
      241 tgctatcacc caacaactga tataaataaa ttacaagcaa atgagagcgt tatcaccttt
      301 tatgatcgga ggaggcagcc atgctgaaaa gtttttcgtt gcaggaaaga agttggatca
      361 tgtatgattg ggccaattcc gcctattcgg tcatcatctc ctcggtcatt ttgccacttt
      421 tttataagtc gatcacaagt ggggaaggaa tcgccccgaa cttggcggat tcttattggg
      481 gctatgcaac ctctgccgca actttggcta tcgccgtatt cgcgccgatc ctcggtacga
      541 tcggggatta tccgaaatgg aaaatgcgac tgttcaaaag tttcttcttg atcggtgttg
      601 ttgcaacggc tgcgctcagc tttacggacg attggcgcct gctgcttgcc ttctatatgc
      661 tcacgacaat cggcttttcg ggcgctaaca ttttctatga cgcctttttg gtggatgtcg
      721 cgacagagga tcggatggat cgggtctcca cttacggatt tgcgatgggt tacatcggtg
      781 gcagtaccat cccattcgtg ctttcgatcc tgctgatcat gttcggcgag aaaatcggca
      841 ttcccaggac gactgccatc aaagcctcct tcctgttcac ggcattttgg tggatcgtct
      901 tcacgattcc gatgctgcgt aatgtgaaac agcgctattt cctcccgccc accgaacata
      961 tcatccatga cagtttcgcc cgccttaaca aaacgctgca ccagatccgc agccacaaga
     1021 aaatgttcct gttcctgatt gcctacttct tttacatcga tgggctgaac acgatcatcc
     1081 acatggcagc tgtcttcggc gacagtatcg gggtcgtgtc tgatgtgctg atgattgctg
     1141 tgctggttat tccgatcctg tcttttcctt tcacgatcct ctacggcgca ctggccaagc
     1201 gcttcggcag caaaaagatg attcttgtgg gcatttgtat ttatctgctc gtctgtctgc
     1261 tggctttccg catgacttcc gccgttgaat tttggattct cgccatcttg gtcgcaactt
     1321 cgcaaggcgg tatccaagca ctttcacgtt cctatttcgc caaacttgtg cccaaggaga
     1381 acgccaacga atatttcggt ttctacaata tcctcggcaa atttgccgcc atcatcggcc
     1441 cagcgctcta tgcgctcacg agtcaattga ccggtgactc acgcaacgga ctcgccagta
     1501 tttctctcct cttcatcatt ggaggcgtgc tgatgctgcg gacagaggga aattaatccc
     1561 gcgttgcata tgatccacac ccgaaaaggc tgacttccac gcggaatcag cctttttagg
     1621 ttctatcttt tactgacgaa cccagacaac ggacgtttga cgagttccgt gacgacaaga
     1681 taagaaacgg cgatccccaa aatgatcccc aaatgcgcca ttgtcggcgg ctggaattca
     1741 aagaaggcgg cagcagcggg aatcatcggc aatccgatgg caagtgccat ggcgatcacc
     1801 gaaaggatca cgatcgacgg cgccggccag ccggccttgg tgatcggagc caaggagcgg
     1861 atcgacaaca tcaataatat ctccgtcaac acgctggcaa tgaaccagtt ggtctgcagc
     1921 actgccgggg aaatcctgta gaatagaccg aagcagatga aatcgaagac ggtgctgacg
     1981 agccccatcg tgacgaaaat gatgtagagg gaatgcagat cataccgttg cggtcgttct
     2041 acttcctgag ccgatacgcg atcaaaggcg atggccatca tcggaaaatc ggaaagcaga
     2101 ttcaggagca gcagctgttt cggcagcatc ggcaaaaagc tgatgaagag cgagccgatg
     2161 gcgaccgcat agaaattgcc gaagttcgag atcaaagtgg cgcggatgta tttcatcgta
     2221 ttggcgtgcg tttcccgccc aaagcggatg ccttcgacga ttacccgcag atcgttctga
     2281 agcaatacga tatcagcggt ctcgcgggcg acatcggccg ccgattgcac gaccatcgac
     2341 acgtgcgccg ccttcaaggc cggtgcatca ttgatccctt cacccaaata gccgactgtg
     2401 aattgttctt tcagcaattg gattagttcc agtttttgct ccggtgtcgt ccgggcaaag
     2461 acgcgaattg aagacagacg tttcttcctt tcggccagtg gtaatgtcaa aaaggcggca
     2521 gcctctgtga cttcagcgct ctccgtcacc aaaccggctt cccttccgac tgcctcggcc
     2581 accttcaggg catccccggt gatgaccgta atggcgatat tcatctgttt tgcggccgct
     2641 acggtctcaa tagtggaatc cttgatgcgg tccacaaaag agacaaatcc gccgaaacgc
     2701 gcaccgctgc cgtcgtcgta gctcacgccc agtacccggt ggccttcctc ttcctctgcc
     2761 tgcagccaat ccgcaacagt atcgcgggaa atcaatcctt ccccgaagaa atattccggg
     2821 ctcccgcggc ggatatgcag tgttgttccg tctacctgtt tcacgactgc accgttgccg
     2881 cgcnnnnnnn nnnnnnnnnn nnnnnaaggc ctcctcctgc gcgagactgt agcgctccac
     2941 aatctgccgt tgcgcttgcg ttagggcctc atcgcttgcg tggtcgaaag gttccggtat
     3001 acgctcatgc agattgatgg cggccaagcg cgccaagacc agtggatcgt aaggggattc
     3061 cgccatcgga taaacattgc tgaacacaag atgattttcc gtgattgttc cggttttgtc
     3121 ggtgcacaac agattgactg atcccaaatc ctggaccgcg gaaagccgct tgacgatgac
     3181 atcgcgtttg gcgaattc
//
LOCUS       DAOMUJ010000184         3178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8643_length_3178_cov_2.458854, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000184 DAOMUJ010000000
VERSION     DAOMUJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8643_length_3178_cov_2.458854"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1318)
                     /locus_tag="PLT73_05520"
     CDS             complement(161..1318)
                     /locus_tag="PLT73_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRM19873.1"
                     /translation="MIVNRNTIREQNETIVLTAIINHPNTSRAAISHDSGLNKATVSE
                     IVKKLLKEKLVVELGTGQSSIVGGRKPVLLKVNANGGYAMSLTITQTKISSLVCNLQG
                     KIVAQYDLVRKVEASNIIDSIEEVVLYHRKNLNKTPFRFVGIVVSIDGFVHEDEIVAS
                     TNKMLEHLTAKHLESDAIDCPIYLENQNNLAVIAEAVFAHSPGNIISIDLDEGIGSGI
                     LLQNRLFRSKNSLAGNLGHTILYPFGKACDCGKQGCLNQYCSTGALVAEIRILKNDSS
                     LHLTDLIALYKTGDEDAKNVVGHLVLHMGVAISNVVSLFDPEKVYLNSDLFRALPECV
                     TEIQTELNRTSGHNIPVSLSSLGKNGALLGGIALALQHFFQVPQLQLHFDE"
     gene            1757..2497
                     /locus_tag="PLT73_05525"
     CDS             1757..2497
                     /locus_tag="PLT73_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM19874.1"
                     /translation="MLGLNGIEWMMLIIAAIIIGFSKTGIQGATIPAVALVAILFGGK
                     ASAGIMLPMLMLGDLIAIFQYGKQGKFRDVLKLLPAAVMGIAIGAITGNYLDDKQFKA
                     LLGIVVLICLGLLIYREIAKRVLPLPKNPIFHWMVGAVSGFSSMVGNAAGPIFNVYLL
                     SQDLTKNKMIGTTAWFFLLMNIVKLPFHIFMWRTVTWGTLRYMLLMIPFIALGSWMSI
                     RFVRKINEVWYKRIIMIMTAIAAMRLFI"
     gene            complement(2582..>3178)
                     /locus_tag="PLT73_05530"
     CDS             complement(2582..>3178)
                     /locus_tag="PLT73_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRM19875.1"
                     /translation="LIGLQINGWSFHGLKRIIRHSGQLSTDYLELGGLGATLINMALL
                     GFLATTYILLMGGEINGPVLGGIFTVIGFGAFGKNVKNVLPILIGVTLMGRLNYQDNQ
                     STIVLISALFGTTLAPLAGRYGNIAGIIAGAMHLTLVMNIGYLHGGVNLYNNGFSGGL
                     VASILVPILEAFQLHRTNQRALRRPVDPADEVEVDRTN"
BASE COUNT          826 a          674 c          780 g          898 t
ORIGIN      
        1 tcgtttcatg aaaccggata catgtatcta catataaaat gttatattaa taatcaacaa
       61 aaagcaatag tgtgtcaaaa aaaacgtcac acccgaaagg atgtgacgac agaaagaggc
      121 tgattcatta tgagtattgt tctgcagatt gtccgaaact ttattcatca aaatgcagct
      181 gcagctgagg aacttggaag aaatgctgga gtgctaatgc aattccgcct aaaagagctc
      241 cattttttcc gagcgaggac aaactgaccg gaatgttatg tccggatgtt cggttcaatt
      301 ccgtttggat ttctgtcaca cattccggta atgcacggaa aagatcgcta ttcagataga
      361 ctttttcagg atcgaacagg ctgacgacat tgctgattgc gacgcccata tgcagcacga
      421 gatgtcccac tacatttttg gcatcttcat cgcctgtctt gtacagggca atcaggtcgg
      481 tcaaatgcag tgaggaatcg ttcttcagga ttctgatttc cgcaaccaaa gctcccgttg
      541 agcaatattg gttcaggcac ccctgcttgc cgcagtcgca agcttttccg aatggataga
      601 gaatcgtgtg ccccagattc ccggccagac tatttttgct gcggaacaat cggttctgca
      661 acaaaattcc ggagccgatg ccctcgtcca gatcgatact gatgatgttc cccggagagt
      721 gggcgaatac cgcttcggca atcacggcca gattattctg attttccaga tagatcggac
      781 agtcgatagc gtcgctttcc agatgtttgg ccgtcagatg ctccaacatt ttgttagttg
      841 atgctactat ctcatcttca tgcacgaagc cgtcgattga cactacaata cctacgaagc
      901 gaaacggtgt tttattgagg ttcttcctat gatagagaac gacttcttcg atgctgtcga
      961 tgatattgct ggcctcaact tttctgacca aatcatattg ggcgacgatc ttgccttgca
     1021 gattgcagac gagggaagaa atctttgttt gtgtaatggt caaactcatc gcgtatccgc
     1081 catttgcatt caccttcaac aacaccggtt ttctcccacc aacgatggaa ctttgacctg
     1141 taccgagttc gacgacaagt ttttccttaa gaagtttttt gacaatttca gatacggttg
     1201 ccttgttgag tccggaatca tgagagatgg ccgctcgtga agtattggga tgattgatga
     1261 tggctgtcaa aacgatggtc tcattttgtt cccgtatggt gttgcggttg acgatcatgg
     1321 tgtgtcacac cctttacaga tttttttgag gtttgggcgc aaaaaaagat gacgaatctg
     1381 aaaaaaatac aaggatgggc tattttggag caaggtaaaa tgtgttccgg atagtcgctt
     1441 ggactcagga gtaccttgta tagaagtcag aagcttagtg gtgaatgcat ttacatagtt
     1501 gtttaaaaca aggtgggctc aataaagagc gatggttgag ggaatcagtc gttgcgggca
     1561 tacgtctttg gttatatttt aacacttttc actggcaatt gcgaggtttg gatgacctcc
     1621 taacccaaat atcgcgcact ttttttcaag gaggtcaaga ggttaagcgt tttctggatt
     1681 gtgggctctg agtcattgtt atttaattag aaaatcggta aaatgaaagg aaccacggga
     1741 gggggcatat atcagaatgt tgggtttaaa tgggattgaa tggatgatgc tgatcattgc
     1801 ggcaatcatc atcggttttt cgaaaaccgg catacaaggc gcgacaattc cggcggtggc
     1861 gctcgttgcc attcttttcg gtgggaaggc gtcagccgga atcatgttgc cgatgctgat
     1921 gctgggggat ttgattgcca tctttcagta cggcaaacag ggaaaattca gggatgtgct
     1981 gaaattgttg cctgccgccg taatgggtat cgctataggc gccatcacgg gtaattatct
     2041 tgatgacaag cagttcaaag ccttattggg catagttgta ttgatttgct taggactttt
     2101 gatttacaga gagatcgcca aaagagtttt gccgcttcca aaaaatccaa tcttccattg
     2161 gatggtcggg gctgtcagcg gtttttcttc gatggtaggc aatgccgcag gacccatttt
     2221 caatgtctat ttgctttcac aagatctcac caagaataag atgatcggaa cgacggcttg
     2281 gttttttctg ctgatgaata ttgtgaagtt gccattccat atctttatgt ggcggaccgt
     2341 cacttgggga acactacggt atatgttgct gatgattcct tttatcgcat tggggagttg
     2401 gatgagcatc agattcgtcc ggaagataaa cgaagtgtgg tacaagcgca tcatcatgat
     2461 catgaccgcg attgcggcga tgcgtctgtt catttaaaaa gagacacctt atgaatggac
     2521 tttagtcccc tcgtaaggta tctctttttt ctggattaaa aaatctgact ggatatttct
     2581 gttaattagt gcgatctact tcaacttcat cagccggatc gactggtctg cgcaaggcgc
     2641 gttggttggt ccggtgaagt tggaatgctt caaggatggg gaccagtatc gaagcgacga
     2701 gtccacccga aaatccattg ttgtagaggt ttacgccacc atgaagataa ccgatattca
     2761 tgactaacgt cagatgcatg gctccggcga tgatgcccgc aatgttcccg tatcggccgg
     2821 ccaacggtgc caaagttgtt ccgaacagtg ctgaaataag gacgattgtt gattgattgt
     2881 cctgatagtt aagtctgccc atcagggtga ccccgatcag aatcggcaag acattcttga
     2941 cgtttttccc gaaagcgcca aagcctatga cagtaaagat gccacccagg accggaccat
     3001 tgatttctcc acccatcaac agaatgtatg tcgtagccag gaatcctagc aatgccatat
     3061 tgatgagcgt cgcacctaaa ccgccaagtt ccaggtaatc ggtggacaat tggcccgaat
     3121 ggcggatgat ccgcttcagc ccatggaagc tccatccgtt gatctgcaag ccaatcag
//
LOCUS       DAOMUJ010000185         3169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8686_length_3169_cov_2.183365, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000185 DAOMUJ010000000
VERSION     DAOMUJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8686_length_3169_cov_2.183365"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLT73_05535"
     CDS             <1..674
                     /locus_tag="PLT73_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM19876.1"
                     /translation="YIHTYSLIHDDLPAMDDDDYRRGRLTSHKVFGESTAILAGDALL
                     TAAFEILAQSSDIPAEKRIKLIDLLAKASGPNGMIAGQMDDVEGEKKALTIEELKQVH
                     DKKTGALIRYAVTAGCLIADADELFITEMERYSRHLGLAYQIHNDLKDVVLDEAETGK
                     TIHHDAALNKNTYPSLLGIAGAIQELNDVIRQAEDCLNRVVLSNGDTENKIDVFRQIL
                     DYVRI"
     gene            687..1529
                     /locus_tag="PLT73_05540"
     CDS             687..1529
                     /locus_tag="PLT73_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRM19877.1"
                     /translation="MEKERLDLLVVQQGLTDSREKAKRLIMAGQIYDENNQRYDKPGE
                     KISVEKTLHIKGETLKYVSRGGLKLEKAIALFQVNPKDKIFLDIGSSTGGFTDVALQN
                     GAKLCYALDVGYNQLDWKLRQDERVEVMERVNFRYSKLEDFEKGRPEMASIDVSFISL
                     KLILPVLKEIIAVGGDVVALIKPQFEAGRGKIGKKGIVSDPAVHNEVLRDILEFASLT
                     GFDVKNLSFSPITGGTGNIEFLVHLEANDHAPGIISPEIDSQQVVKVAYLQLKSKSIS
                     ISDK"
     gene            1615..>3169
                     /gene="recN"
                     /locus_tag="PLT73_05545"
     CDS             1615..>3169
                     /gene="recN"
                     /locus_tag="PLT73_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRM19878.1"
                     /translation="MSFDEGMTVLTGETGAGKSIIIDAVGLLAGGRGSNDLIRYGENK
                     TVIQGLFSIPENAKTVSVLESYGIEADGNQVLLQRELSRNGKNISRVNGTIVTVATLR
                     EVGETLIDIHGQNEHQELMDPQKHIDLLDQFAGNELREVKTTYLNTYAKYVDTRKQLI
                     KLKTDEKETVQRIDLLTFQIQEIEQAQLKDAAEDEKLEEERNLLVNYQKVMQALTSAY
                     DSMQNSEENGIDKIGLGMAALEKVQDINPVYQAIASSVSAAYYQLQECAGDILSEIEQ
                     LSYDEGRLNEIENRLELIRQLKRKYGNTISEVLGHYEKISQELDLIENRESYLEKLNV
                     DYNKAKEEILAVGRQLTTIRKKAATVLEEQIQLQLKELYMDKVLFKTVFHEQPGKVQI
                     TDNGLDQVEFYIATNVGEPLKALAKVASGGELSRMMLAMKAIFTKTQGITSIIFDEVD
                     TGVSGRVAQAIANKIHLVAGYSQVLCITHLPQVAAMADQHLYIEKEIIADRTKTHVKS
                     LFGAERINEV"
BASE COUNT          976 a          680 c          831 g          682 t
ORIGIN      
        1 aatatatcca tacgtattcc ttgatccatg atgatctgcc ggccatggat gatgatgatt
       61 acagacgggg gcgcttgact tcccataaag tattcggaga atccaccgca atccttgcag
      121 gtgatgcgtt gttgacggcg gcatttgaaa tccttgcaca atcctctgac attccggcgg
      181 aaaaaagaat caagctgatc gacttgttgg cgaaagcctc gggtccgaac ggcatgatcg
      241 ccggccagat ggatgatgtt gagggtgaaa agaaggcact gacaatagaa gagttgaagc
      301 aagtccacga taaaaagacc ggcgcattga taaggtacgc tgtcacggcg ggatgcctca
      361 tcgcggatgc ggatgaactg ttcataaccg agatggaaag atactccaga cacttaggtt
      421 tggcatacca aatccacaac gatctgaagg atgtcgtgtt ggatgaggcc gagacaggca
      481 aaaccatcca tcatgatgcc gcattgaaca aaaatacata cccatcctta ttgggtatcg
      541 ccggagccat tcaagaactg aacgacgtca ttcgccaagc ggaggactgt ttgaatcgag
      601 tggtactatc caatggcgac acggagaaca aaatagatgt attccgccaa atattggatt
      661 atgtcagaat ataacgggag attaccatgg aaaaagaaag attggattta cttgtagtac
      721 aacagggctt gacagattcc cgtgaaaaag ccaaaagatt gatcatggcc gggcaaatct
      781 atgacgaaaa caaccagcgc tacgacaaac cgggcgaaaa aatttccgtc gagaaaacgc
      841 tccacataaa aggagaaaca ttgaaatacg tgagccgtgg cggattgaaa cttgaaaaag
      901 ccatcgcttt attccaagtg aaccctaagg acaaaatctt tttggatatc ggcagttcaa
      961 cgggcggttt tacggatgtc gcgctccaaa atggagcgaa attatgctac gcactggatg
     1021 tcggctacaa ccaattggat tggaagttgc gccaggatga gcgcgtcgag gtgatggaac
     1081 gggtgaattt ccgttacagc aagcttgagg attttgaaaa aggacggccc gagatggctt
     1141 ccatcgatgt atccttcatt tcgttgaaac tgattttgcc tgtcctgaag gaaatcatcg
     1201 ctgtcggcgg agacgtcgtt gcgctgatca aaccgcaatt cgaagctgga agagggaaaa
     1261 tcggcaaaaa aggcatcgtc agtgatccgg cagtccataa tgaagtgttg cgggacatct
     1321 tggagttcgc ttcgctgacc gggtttgatg tgaaaaatct gagcttttcg cccatcacag
     1381 gtgggaccgg aaacatcgaa tttttggtcc atttagaggc taatgatcat gcgccgggaa
     1441 tcatatctcc ggaaatagac agccagcaag tcgtcaaagt ggcttacctg cagcttaaga
     1501 gcaaatcaat ctcgatttcc gataaatagg tgactaagga accattgcga aggggaatac
     1561 ggaatgttac aagagttgtc cattaaaaat tttgcaataa ttgaaaatct tcaagtgagt
     1621 ttcgacgagg gtatgaccgt gctgactggg gaaaccggcg cagggaaatc catcatcatc
     1681 gatgccgtag gtctgttggc aggcggaaga ggatccaacg atttgattcg atacggcgaa
     1741 aacaagacgg tcatccaagg gcttttcagt attcccgaaa acgccaagac agtttccgtg
     1801 ttggagtcat acggaatcga agcagacggg aaccaagttt tgctgcagcg ggaactttcc
     1861 cgaaacggca aaaacatttc ccgcgtcaac ggtacaatcg tgacagttgc gactctgcgt
     1921 gaagtaggcg aaacgctgat cgatatccac gggcagaacg agcatcaaga attgatggat
     1981 ccgcaaaaac atatcgacct gctggatcag tttgccggga atgaactgag agaagtcaaa
     2041 acgacctatc tcaatactta tgccaaatat gtggatacac gcaagcaact gatcaaactg
     2101 aagacggacg agaaagaaac ggtccaaaga atcgacttgt tgactttcca aatccaggaa
     2161 atcgaacaag cgcagttgaa agatgctgca gaggatgaaa aattggaaga ggagcggaac
     2221 ctgctcgtca actaccaaaa agtcatgcag gctttaacga gtgcgtacga ttcaatgcag
     2281 aacagtgaag agaacggcat cgacaaaatc ggtctcggca tggcggcgct ggaaaaagtg
     2341 caggacatca atccggtcta ccaggcaatc gcatcctcgg tctcggcagc gtattaccaa
     2401 ctgcaggaat gcgcaggtga catcctgtcg gaaatcgagc aattgtccta cgatgaaggg
     2461 cgcttgaacg aaatcgagaa ccgattggaa ttgatccgcc agttgaagcg gaaatacgga
     2521 aacacaatca gtgaagtgct ggggcattac gaaaaaattt cccaagagct ggatctgatc
     2581 gaaaaccggg aaagctacct tgagaaactc aatgtggact acaacaaagc aaaagaagag
     2641 atcttggctg tcggcagaca attgaccact atccggaaga aagctgcaac cgttctggaa
     2701 gaacagatcc aactgcagct gaaggaattg tacatggaca aggtcctttt caagacggtg
     2761 ttccatgaac aaccaggaaa ggttcaaatc actgataacg gcttggatca ggtggaattc
     2821 tatattgcga cgaatgtcgg cgaaccatta aaagcattgg cgaaggttgc cagcggtggc
     2881 gaactttcaa ggatgatgtt ggcgatgaaa gccattttca ccaaaaccca aggcatcacc
     2941 agtatcatct ttgatgaagt ggatacgggt gtctcgggca gggtagcgca ggcgatagcc
     3001 aataagatcc acttggtggc cggttactca caagtgcttt gcatcacgca tcttccgcaa
     3061 gtggcggcga tggcggatca gcacctttat atagaaaagg aaatcattgc cgatcggaca
     3121 aagacgcatg tcaagtcgct gttcggtgct gaacgcatca acgaagtgg
//
LOCUS       DAOMUJ010000186         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8716_length_3162_cov_1.826521, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000186 DAOMUJ010000000
VERSION     DAOMUJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_3162_cov_1.826521"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..647
                     /locus_tag="PLT73_05550"
     CDS             150..647
                     /locus_tag="PLT73_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM19879.1"
                     /translation="MKTEKNSTYRLVILALFIAFTVVMTLLFSIPIPFGYVNLGDMVL
                     LLAGLSFGSSAGFIVGSLGSMLADLFAGYAFYAPITFVVKGLEGFFAGWLFQKMDHKK
                     PLVATVIAGVWMAIGYAIGDTILFSFGAAIASFPLNIAQGLVGAFLSTLLYRWLNPYI
                     AKKLK"
     gene            complement(708..1631)
                     /locus_tag="PLT73_05555"
     CDS             complement(708..1631)
                     /locus_tag="PLT73_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM19880.1"
                     /translation="MDVSVVFSRMIMLVMIMFVGYLATVMKVFDKHANANFAALITYV
                     TVPALVIASVEGAGEVGTKSDTLFVLLTATLMYLFTVGLAKFSPKLFRADKETEGIIE
                     FLTIFTNNGFMGLPVVQAIFGTGALFYASLYGIPNNLLIYSLGVFLIAKGSKKSRASW
                     KAILLNPGNLASLFAVFVFLFDVKLPALVMDTATSIGNITSPLAMIIIGASLADIDIL
                     GAFKEWKIYLFAFYRMIVIPLFLWWATKGILTNPTMLGILVIISAMPGPAMAVALSMQ
                     YGGNTAFSTRYVFISTLLSVFTIPLLAALLF"
     gene            1840..>3162
                     /locus_tag="PLT73_05560"
     CDS             1840..>3162
                     /locus_tag="PLT73_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRM19881.1"
                     /translation="MQKKLTILQINDTHSYLELHNELFYERDKLAFRKAGGFARLQSL
                     IKDFRSKNPTLVFDNGDTIHGTYEAIATKGWHMLPVLNEVGFDAMTFHWDTGFGPANL
                     KKIGKQLDYPILAINAYEKESGKLAFEPYKILSVGELSIGVIGIACNIIDKTMPPHFS
                     EGLSFTLGREELPRYVAELKEKEVNLIIVLSHLGFPQDLKLMSEVEGVAICLSGHTHN
                     RQEHIVTVGETAVIQSGSHGSFLGALELTVEDGAIQSIEHQLIPVTEDIPEDAEMKVL
                     VEDALSPYREKLELKVGETKKVLHRGWSVETTMDNFLLDAILFHTKTDLAFSNGWRYG
                     VPILKGPVTLRELYQIIPMDPPISTAILTGSEIVALLEETLESTYAADPFNQMGGYLK
                     RAMGLHAYLKLENPKGTRVQKLFIAGEEVIPDKEYNVSYVTNQGVPKKF"
BASE COUNT          831 a          807 c          779 g          745 t
ORIGIN      
        1 aaaaactcaa ttattcccat tctatcatct tttcattgga ttttctgatt tttccatcat
       61 tttggaaatt attgtgaaaa cgatgagaaa aagagtgcaa acttctgcat aagggctata
      121 ataaaaaata atgcttggag gagagttgca tgaagaccga aaaaaatagt acttacagat
      181 tggttatttt ggccttattt atagcattta ccgtcgtcat gaccttattg ttcagcatcc
      241 cgatcccttt cggctacgtt aacttgggag acatggtgct cctattggcc ggtctgtcct
      301 tcggaagcag cgcgggcttt atcgtcggtt cactcggcag catgttggct gacctgttcg
      361 ccggttatgc cttctatgcg ccgattacct ttgtcgtgaa aggtttggaa ggattctttg
      421 ccggctggtt gttccagaaa atggatcaca agaagccgtt ggttgccacg gtgatcgccg
      481 gtgtgtggat ggcgatcgga tacgccatcg gggacacgat cctattcagt tttggtgccg
      541 cgattgcttc cttcccgttg aacatcgcgc agggcctcgt cggggctttc ttgtccaccc
      601 tcttgtaccg ttggttgaat ccttatattg cgaagaaatt gaaataacga atgtgcgccc
      661 actccgtatg gttagcggag tgggcgttct ttgtgcatct atgctgctca aaacagcagc
      721 gcagccaaca gcgggatcgt gaacaccgac agcaacgtcg aaatgaagac gtatcgcgtt
      781 gaaaaggctg tgttgccacc gtactgcatc gacaatgcca cagccatcgc cgggccaggc
      841 atggccgaaa tgatgacgag aatccccagc atagtcggat tcgtcaggat gcccttcgta
      901 gcccaccaga ggaacaacgg aatcacgatc atgcggtaga acgcaaacag atagattttc
      961 cattccttga aggcacctag gatatcgatg tcggccaatg acgcgccgat gatgatcatc
     1021 gccaatgggg acgtgatatt cccgatgctg gtggcggtgt ccatcaccaa cgccggcaat
     1081 ttaacgtcga acaggaacac gaacaccgcg aacagcgaag cgaggttgcc gggattcagg
     1141 agaatcgctt tccagctcgc ccgcgacttt ttcgagcctt tcgcaatcag gaagactccc
     1201 aaagagtaga tcaacaggtt gttcgggatg ccgtagagcg aggcataaaa aagggcgccg
     1261 gtgccgaaaa tagcctgcac gaccggcaat cccatgaaac cattgttcgt gaagatggtc
     1321 aggaactcga tgatcccttc cgtttcctta tccgcgcgga acagtttcgg actgaacttg
     1381 gccaatccga ccgtgaaaag atacatcagg gtagcggtca acaagacgaa gagcgtgtcc
     1441 gacttcgtac ctacctcgcc tgcaccttcc accgatgcaa tcacaagcgc cggcaccgtg
     1501 acataggtga tcaacgctgc aaaattggcg ttcgcgtgct tgtcgaaaac tttcatgacc
     1561 gtcgcgagat agcctacaaa catgatcatc accagcatga tcatcctact aaatacaaca
     1621 ctaacatcca aaattcttgt tcctcctctg ccgaccatcc atttatatat ccaaattcta
     1681 acacagttct gcgttgaaat tttattttcc attacgtttc aggattcttc ctgtgccgta
     1741 cagggcataa caatcggtat ctgccgcaca gaatgttaga atagaagcaa ccatataacg
     1801 aatgcttctg tcacatttcg tcctgaaagg tggattacta tgcaaaaaaa attgacgatc
     1861 cttcagatca acgacaccca cagctacttg gagttgcaca acgaactgtt ctacgaaagg
     1921 gacaaattag cgttccgcaa ggctgggggg tttgctcgtc tccaaagcct gatcaaagat
     1981 ttccgcagca aaaatccgac actcgttttc gataacggcg acacgatcca tggcacctac
     2041 gaagcgatcg ctacgaaagg ctggcacatg ctgcccgtcc tgaacgaagt cggctttgat
     2101 gccatgacat tccactggga caccggcttc ggtccggcca atctgaaaaa aatcggaaag
     2161 caattggatt acccgattct cgcaatcaat gcctatgaaa aggaaagcgg caagttggcg
     2221 ttcgagccct acaaaattct gtccgtcggc gaactttcga tcggtgtgat cggtatcgct
     2281 tgcaacatta tcgacaaaac gatgccgccc catttcagtg aagggctttc cttcactttg
     2341 ggccgtgagg agctgccccg ctatgtagcg gaactgaagg aaaaagaggt caatctgatc
     2401 atcgtcctgt cccatcttgg tttcccgcag gacctgaaat tgatgagcga agtcgaaggc
     2461 gtggcgatct gcttgagcgg ccatacccac aatcgtcagg aacacatcgt cacagtaggt
     2521 gaaacagcag tcatccaatc cggctcgcac ggctctttcc ttggcgcgct cgaattgacc
     2581 gttgaggacg gcgcgatcca aagcatcgaa catcaactga ttccggtaac ggaggatatc
     2641 ccggaagacg ccgaaatgaa agtgttggta gaggatgccc tctcccctta ccgcgagaaa
     2701 ttggagctga aagtcggtga gaccaaaaaa gtgctgcacc gcggctggag cgtcgaaaca
     2761 acgatggaca acttcctctt ggatgcgatc cttttccaca cgaaaacaga tctggccttc
     2821 tccaacggct ggcgctacgg ggttccaatc ctgaaaggcc cggtaacgct gagggaattg
     2881 tatcagatca ttccgatgga tccgccgatt tcaactgcta tcctgactgg ttcggaaata
     2941 gtcgctctgc tggaagaaac ccttgagagc acctacgcag cggacccatt taatcagatg
     3001 ggcggctacc tgaagcgggc gatgggcttg catgcttacc tgaaactgga aaatccaaaa
     3061 ggaacccgtg tccaaaaact tttcatcgcc ggcgaagaag tcatcccgga taaagaatac
     3121 aacgtcagct atgtgaccaa tcagggggtg ccgaagaaat tt
//
LOCUS       DAOMUJ010000187         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8765_length_3148_cov_2.541545, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000187 DAOMUJ010000000
VERSION     DAOMUJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_3148_cov_2.541545"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..308)
                     /locus_tag="PLT73_05565"
     CDS             complement(45..308)
                     /locus_tag="PLT73_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRM19882.1"
                     /translation="MKQEIHPNYQPVVFMDTTTGFKFLSGSTKGSSEKVEWEDGNTYP
                     MIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGFKNNEEQA"
     gene            complement(524..895)
                     /gene="traF"
                     /locus_tag="PLT73_05570"
     CDS             complement(524..895)
                     /gene="traF"
                     /locus_tag="PLT73_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HRM19883.1"
                     /translation="MLFNKGNYEEDTKEFIEITAEEFEEKLAAGEKTIIYLGKAVCPY
                     CRKFVPKLDKVRKEKNLTIHYLDSLNTPTDPAIQSLRDRMGVRTVPALVTIDGPGQFT
                     NLNADSSLSEEEITAILDKKS"
     gene            complement(992..2251)
                     /gene="rho"
                     /locus_tag="PLT73_05575"
     CDS             complement(992..2251)
                     /gene="rho"
                     /locus_tag="PLT73_05575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM19884.1"
                     /translation="MEDMLTLHELESKTLKEIYTYARDLKIPYYSQMNKKELSLAVIR
                     AQEEKQGFFMTEGVLDIVAQEGFGFLRPINYSPSQEDIYISTSQIRRFDLRNGDKVAG
                     KARPPKASERYYGLMQVSTVNGKRPEQAKERSHFPGLTPIYPNQQMKLETARTNVTAR
                     MIDLFSPIGFGQRGMIVAPPKAGKTTLLKEIANGITTNHPDVELIMLLIDERPEEVTD
                     IERSVKGDVVSSTFDQQPANHVRVAELVLERAMRLVEDKRDVVILMDSLTRLARAYNL
                     VIPPSGRTLSGGIDPAAFYRPKRFFGAARNIEDGGSLTIIATALVDTGSRMDEVIYEE
                     FKGTGNMELVLSRELSERRIFPAIDIKKSGTRREDLLLDAKTMESIYKVRRSMRGDTL
                     EYTESFLKQMNTTENNEALLKRIALMK"
     gene            complement(2386..>2659)
                     /locus_tag="PLT73_05580"
     CDS             complement(2386..>2659)
                     /locus_tag="PLT73_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM19885.1"
                     /translation="RVNHIPELRRMGANAKVESDMIFMQGPNKLTGAEVTASDLRAGA
                     CLVIAGLMAEGTTTISGIENILRGYDHIVEKLTALGADIKMIEDEE"
     assembly_gap    2660..2669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2670..>3148)
                     /locus_tag="PLT73_05585"
     CDS             complement(<2670..>3148)
                     /locus_tag="PLT73_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM19886.1"
                     /translation="NTILAAVKAKGKTIIENVAKEPEIIDVCILLNNMGAKIRGAGTD
                     IIRIEGVENLHGCRHTVIPDRIEAGTYLAAAAAVGERVLVKNVIVEHIESLIAKMEEM
                     GVDMEIGEDSILVKKSDNLKMVHIKTLPYPGFATDLQQPLTPLLLKASGDGTIIDTI"
BASE COUNT          719 a          785 c          694 g          940 t
ORIGIN      
        1 taaaaagagc tctcccgaaa ccgggaaagc tctcttcaaa aagattatgc ttgttcttcg
       61 ttgtttttga aaccgtattt cttgttgaag cggtccacac ggccatcggc ttgcgtgaat
      121 ttttgacgtc cagtatagaa tgggtgtgaa tcagaagaaa tttctacacg gatcattggg
      181 taagtgttgc catcttccca ttcaaccttt tcgcttgaac cttttgttga acctgataaa
      241 aatttgaatc ctgtagttgt atccatgaat acaactggtt ggtaatttgg atggatttct
      301 tgtttcatgt ctctttcgct ccttttgccc tggattattt actaagccag agttgtcatt
      361 ttgtaataaa aggcctaccg cagggtaatc ctttttaacg ttaaaatgat accacgcaaa
      421 cagcaagaat gcaatgataa attttaaaaa cgcgctcccg ctcttgtagt cagtgccctg
      481 aaaaacacat ttgctacggt ttagtattgt ctagcttcac gggtcagctt ttcttatcca
      541 gaattgccgt aatctcttct tccgaaaggc tgctgtcggc attcagattc gtaaattggc
      601 ccggaccatc gatcgtcaca agcgcaggca ccgtacggac acccattctg tcgcgcagac
      661 tttggattgc agggtctgtc ggtgtgttca ggctgtccag ataatggatc gtcaagttct
      721 tttctttgcg gactttgtct aatttaggga caaatttccg gcaatagggg caaacggctt
      781 tcccgagata aatgatcgtt ttttctccgg cagccaattt ttcttcaaac tcttcagccg
      841 tgatctctat gaactctttt gtgtcttctt cgtaatttcc tttattgaaa agcatttgaa
      901 aacctccagt tttgcgtttt gatagtccca ctataccggg aagctaaaaa aaatgctcat
      961 aatttgctcg cgcgccttga gggtcgcctc gctatttcat caacgcaatc cgcttcagca
     1021 gagcttcgtt gttttcggtc gtgttcattt gtttcaagaa actctcagtg tattccaaag
     1081 tatccccgcg catgctgcgg cggactttgt agatactttc catcgttttc gcatccagca
     1141 ggaggtcttc tctgcgggtg cccgattttt tgatatcaat ggctgggaag atgcgtcgtt
     1201 ccgacaattc acgggaaagc acaagttcca tgttccctgt tcccttgaat tcttcgtaga
     1261 tgacttcatc catccggctg ccggtgtcga caagcgccgt tgcgatgatc gtcagactac
     1321 cgccatcctc aatattccgg gctgcgccaa agaaacgttt tggacgataa aaggctgccg
     1381 gatcgatccc accactcaac gtacggccgc ttggcggaat aaccaagtta taggcacggg
     1441 ccaaacgcgt taggctatcc atcaagatga ccacatcacg tttgtcttca acaaggcgca
     1501 tggctctttc caggacaagt tccgccaccc ggacatgatt tgccggttgc tggtcaaagg
     1561 tcgaggagac aacatccccc ttcacactgc gctcgatatc cgtcacttct tcgggacgct
     1621 catcgatgag cagcatgatc agttcgacat caggatgatt ggtggtgatg ccattcgcga
     1681 tttctttcaa cagcgtcgtt ttccctgctt taggaggcgc aacaatcatc ccacgttgtc
     1741 cgaaaccgat gggcgagaac aaatcgatca tccgagcagt aacgtttgta cgtgcggtct
     1801 ctaacttcat ctgctgattc ggataaatcg gtgtcagccc cggaaagtgc gagcgttctt
     1861 tggcttgctc aggccgtttc ccattgacgg tgctgacttg catcaggccg taataacgtt
     1921 cggatgcttt tggaggtctg gcctttccgg ctactttatc tccattgcgc agatcgaagc
     1981 gtctgatttg cgaagtcgat atgtaaatgt cttcttggct cggcgaatag ttgatcggcc
     2041 ttaaaaagcc gaacccttcc tgagcgacga tatccaaaac accctcggtc atgaagaatc
     2101 cttgtttttc ctcttgagca cggataacgg ccaaggacaa ttcttttttg ttcatttggc
     2161 tgtagtaagg aatcttcagg tctctggcat aggtgtatat ttctttcagt gtcttacttt
     2221 ccaactcatg caaggtaagc atatcttcca aacaattcca ccttcctctc ttatcattct
     2281 gatgatgctt attttaggca agtgtacact ataagatatt caaagcacct tcaaagtcgg
     2341 ttgctgcttt gaaggtgctt tgcatatcgt gtagcgctga gaagtttatt cttcgtcttc
     2401 gatcattttg atgtctgcgc ccaaagcggt cagtttttcg acgatatgat catatccgcg
     2461 caggatattc tcgataccgg aaatcgtggt tgtaccctcc gccatcaagc cggcgatgac
     2521 caagcaggct cctgcacgca aatcgctggc cgtgacttct gcaccggtca gtttgttggg
     2581 accttgcatg aagatcatgt cactttccac cttggcattc gcacccattc tgcggagttc
     2641 cgggatatgg ttgacgcgtn nnnnnnnnna gatcgtatcg atgatggtac cgtcaccgct
     2701 cgctttcaat aagagcggcg tcagtggttg ctgcaaatca gtcgcaaaac caggataagg
     2761 caacgttttg atatggacca tcttcagatt atcggatttc ttgaccagaa tactatcctc
     2821 accgatttcc atatccaccc ccatctcttc catttttgcg atcaggcttt cgatatgctc
     2881 aacaatcaca ttcttcacaa gtacccgctc acccactgca gccgcagcag caagataagt
     2941 ccccgcttcg atgcggtcag ggatcacggt gtgtctgcag ccatgcaggt tttccacacc
     3001 ttcgatacgg ataatatcgg taccggctcc gcggattttt gctcccatgt tgttcaataa
     3061 gatacagaca tcgatgattt ccggctcttt ggccacattc tcgatgattg tttttccttt
     3121 agctttgact gccgccaaga tggtgttg
//
LOCUS       DAOMUJ010000188         3144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8788_length_3144_cov_2.460343, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000188 DAOMUJ010000000
VERSION     DAOMUJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_3144_cov_2.460343"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLT73_05590"
     CDS             <1..698
                     /locus_tag="PLT73_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM19887.1"
                     /translation="VGLAMLAAAKGYKAIFVMPDTMSMERRLLLAAYGAELVLTPGAE
                     GMNGSIARAREIAMQPEHFMPMQFDNSANPAVHEAITGPEIIEAFEGKTPDAFIAGVG
                     TGGTVTGVGKALRKVNPNVEIYALEPTDSAVLSGKPKGPHKIQGIGAGFIPSVLDTTL
                     YNGIVQVSNEQAFEMARRVAREEGVLVGISGGAAIAGAIEVAIRLGKDKSVVTVAPDN
                     GERYLSTPLFSVE"
     gene            892..1920
                     /gene="dusB"
                     /locus_tag="PLT73_05595"
     CDS             892..1920
                     /gene="dusB"
                     /locus_tag="PLT73_05595"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRM19888.1"
                     /translation="MYKIGNIEINNPVAVAPMAGISNAAFRVTVKEMGAGLVVCEMIS
                     DKGIQFRNEKTLRMLHIEPNEYPLSVQIMGGNKDTLVEAAKYVAENTEAAIIDINMGC
                     PVSKVIKAEAGAKWLLDPGKVYEMVSAVVDAVDKPVTVKMRTGWDSDHLFAVENALAA
                     ERAGAAAVAMHGRTRVQMYEGHADWNIFKDVKQALKRIPLLGNGDVKSPEDAKRMMDE
                     TGVDGVMIGRAALSNPWMIKQTVHYLETGEMLPQSTPREKLEIAKLHLDRLTDLKGET
                     VAVKEFRSIAAYYLKGIPRASKTKAAVTSASRQQEVVDLLDRFIFEVEEREASKEART
                     FEKIEINS"
     gene            2028..>3144
                     /gene="lysS"
                     /locus_tag="PLT73_05600"
     CDS             2028..>3144
                     /gene="lysS"
                     /locus_tag="PLT73_05600"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRM19889.1"
                     /translation="MNDQLLIRREKMQALREEGIEPFQNGFVRTHLSATIHQEFDAFT
                     KEEIEAKDETIVSVAGRIMTKRGKGKVGFAHLQDSKGQIQIYVRKDVVGEEDYAIFKQ
                     ADLGDIIGVTGPIMKTDAGEVTIKPTQLVHLTKALRPLPDKYHGLTNVEQKYRQRYLD
                     LISNKESFNKFVQRSQIISEIRTYLNGLGYLEVETPTLHNMAGGATARPFITHHNALD
                     MELYMRIALELHLKRLVIGGMEKVYEIGRVFRNEGIDTTHNPEFTMLEMYTAYTDFHD
                     VMEVVEGIFETVTTKVKGSSTVVYDGTEINLAGPYPRIHMVDAVKAVTGVDFWAPMTD
                     AEAQALAEKHNVPFTKNMTVGHIINEFFEAFVEDTLVQ"
BASE COUNT          930 a          642 c          848 g          724 t
ORIGIN      
        1 gagtgggttt ggccatgttg gctgctgcga aaggctacaa agccattttt gtgatgccgg
       61 atacgatgag catggaaaga cgtttactgt tggcagcata cggcgcagaa ttggttctga
      121 cgccaggagc ggaaggaatg aatggttcga ttgcaagagc aagggaaatc gcaatgcaac
      181 cggaacactt tatgccgatg cagtttgata actcggccaa tcctgctgtg catgaagcca
      241 tcacaggtcc ggaaatcatc gaggcttttg aaggcaagac gccggatgct tttattgccg
      301 gagtgggaac gggaggaacc gttacaggag tcggcaaggc tttgcgcaaa gtcaatccca
      361 atgtggagat atatgcattg gagcctaccg attcggctgt gttgagcgga aagccgaaag
      421 gcccgcataa aatacaaggt atcggagccg gattcatccc gtctgtattg gatacgacac
      481 tgtacaacgg tattgtccaa gtctccaatg aacaagcttt cgaaatggct cgacgcgttg
      541 cgcgggaaga aggcgttttg gtggggattt caggcggagc tgccatcgct ggtgcgattg
      601 aagtggccat ccgtttaggc aaagacaaat ctgtcgtgac ggtggcaccg gataacggag
      661 aacgctacct gtccacgcct ctgttcagcg tagaataata gatggaaaaa gaagggcagg
      721 ccgatcggat ggcttgttct tttttcttat cggcgagtca ccatacataa ttggataaga
      781 tatttttctt ttcttacgga ttgtaagatg atagaatggt ccaagagttt tttagcatcc
      841 aaaaaatgga tggaatcaac ataaataaat aaataaaaaa gggagtaaaa aatgtataaa
      901 atcggtaata ttgaaatcaa taaccccgtt gcagtggcac ctatggccgg gatcagcaac
      961 gcggccttcc gtgtcacggt taaagaaatg ggcgcgggct tggttgtctg cgagatgata
     1021 agcgacaaag ggattcaatt ccgcaatgag aagaccttac ggatgctaca catcgagcct
     1081 aacgaatatc cgctgagtgt ccaaatcatg ggtggcaata aggataccct ggttgaagcg
     1141 gcaaaatacg tggcggaaaa cacggaagcg gccatcatcg acatcaacat gggctgtccc
     1201 gtcagcaaag tgatcaaggc ggaagcagga gccaagtggt tgttggatcc gggcaaagtc
     1261 tatgaaatgg tttccgctgt cgtggatgcc gtggataagc cggttaccgt aaaaatgcgg
     1321 acgggttggg attccgatca tctttttgca gtggagaatg ctttggcggc agaacgcgct
     1381 ggtgcagccg cggttgcgat gcatggacgc acgcgtgtac agatgtatga aggacacgcc
     1441 gactggaaca tcttcaagga cgttaaacag gctttgaagc gtatcccgtt attggggaac
     1501 ggagatgtga agtcacctga ggatgcgaaa cggatgatgg atgaaacggg cgtggatggt
     1561 gttatgatag gccgcgctgc cttgagcaac ccttggatga tcaaacagac agtccattat
     1621 ctggaaactg gagaaatgtt gccgcaatcg accccgcgcg agaaattgga aattgcaaaa
     1681 ctgcatttgg acagattgac cgatcttaag ggcgaaaccg tagctgtcaa agagttccgt
     1741 agcatcgcgg cttattactt gaaaggcatt ccgcgtgctt caaaaacaaa agctgcagtc
     1801 acatcagcat ccagacaaca agaagtagtt gacttgttgg atcgcttcat tttcgaagta
     1861 gaggaacgcg aagcttcaaa agaagcgcgt acattcgaaa aaatcgaaat aaatagctaa
     1921 gacggcttga tttttcactc ttttattggc aatattaaga agattatgaa tcaagaagat
     1981 ttaagaacgg agtgacctca ttgagtcaag aaaaacattc agaagaaatg aatgaccagt
     2041 tgctgattcg ccgtgaaaag atgcaggctt tacgcgaaga aggaattgaa ccctttcaaa
     2101 atggttttgt aagaacacat ttatctgcaa caatccatca agagttcgat gcttttacga
     2161 aagaggaaat cgaagcaaaa gatgagacca tcgtttcagt agccggcaga atcatgacaa
     2221 aacgcggaaa agggaaggtt ggctttgctc acttgcagga cagtaaaggc caaattcaga
     2281 tttacgtgcg caaagacgtg gttggggaag aggattatgc gatctttaag caagcggatc
     2341 ttggggacat cattggcgta actggcccga tcatgaaaac tgatgccggt gaagtcacca
     2401 tcaaaccgac gcaacttgtg catttgacga aagcattgcg tccgttgcct gacaaatatc
     2461 acggcttgac gaatgttgaa cagaaatatc gtcaacgtta tcttgacttg atcagcaaca
     2521 aagagagctt caataagttc gtgcaacgta gccagatcat cagcgaaatc agaacttact
     2581 tgaacgggct gggttatttg gaagtcgaaa caccgacatt gcataacatg gcaggtgggg
     2641 ctaccgcaag accgttcatc acgcaccata atgcattgga catggaattg tacatgcgca
     2701 tcgcgttgga gttgcatctg aagcgccttg tgatcggcgg catggaaaag gtttatgaaa
     2761 tcggtcgtgt attccgtaac gaaggaatcg atacaaccca caatccggaa ttcacgatgt
     2821 tggaaatgta tacagcctat acggacttcc acgatgtgat ggaagtggtc gaaggcatct
     2881 tcgagacggt tacaacaaaa gtcaaaggct cttcaacagt agtctatgac ggcaccgaaa
     2941 tcaacttggc cggcccatac ccgcgcatcc acatggttga cgcagtcaaa gccgttacag
     3001 gcgtcgattt ctgggcaccg atgacggatg cagaagcgca agctttggcg gaaaaacaca
     3061 atgtgccttt cactaagaac atgacggtag ggcatatcat caatgaattt ttcgaagcgt
     3121 ttgtggaaga tacattggtt cagc
//
LOCUS       DAOMUJ010000189         3119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8895_length_3119_cov_2.608681, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000189 DAOMUJ010000000
VERSION     DAOMUJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8895_length_3119_cov_2.608681"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1016
                     /locus_tag="PLT73_05605"
     CDS             201..1016
                     /locus_tag="PLT73_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM19890.1"
                     /translation="MTIIEEIQNQYSAFSANEKKIADYILQNKASIKNMNIKDLAAKA
                     DTSTSSITRFCKRINIDGFVDMKIALRSLASGHPSKEMPTVYDDIYGYYTQVIAETNQ
                     MIEDQAIETVGDWIKNANKTVIYGIGSSGFTAAELAQRLIRMGLNATGVTDSHLMIIN
                     SSIIKEGELVIAISNSGETPELIASLEIAKKKKAKIIALTSFKNSSLTKLADVTCFGY
                     HSRFVNNTVFVNTQFGMMYLIDVISTFLLQDHDFQYNMDLTRANVQGYSKKTE"
     gene            1085..2230
                     /gene="nagA"
                     /locus_tag="PLT73_05610"
     CDS             1085..2230
                     /gene="nagA"
                     /locus_tag="PLT73_05610"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011667314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRM19891.1"
                     /translation="MKRVIIHAEVYTGETVIFDGFVRFEKEISAVGKMSEYVPESGEE
                     VIDAAGSRLVPGFIDVHSHGGYGVDNMDGDAEDINRMIAAMHREGITSYFPTTMTQSS
                     ENIERALIGIRKASEKNPVIQGIHLEGPFISVVHKGAQPEEHIKLPDAALMRKWQDLS
                     GGLIRLVTYAPETTDATAFEKYCLDNRIVLSVGHSDALRSQLRKSKAAHVTHLFNAQR
                     GLHHREPGVTGHAFLEDDIYAEMIVDGLHIDPEMVKIAFKQKGADRIELITDAMRAKG
                     MGDGISELGGQKVIVKEGQARLESGNLAGSVLGFQEAFRNIIAFTGCSILDAVKMSSV
                     NQAREFDLVRKGGIAPGKDADMVLLSESFAVERTISYGQTVYKNNRK"
     gene            2241..2948
                     /gene="nagB"
                     /locus_tag="PLT73_05615"
     CDS             2241..2948
                     /gene="nagB"
                     /locus_tag="PLT73_05615"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM19892.1"
                     /translation="MEIIIKATPEEASRYAFEKIREEMERGAKVLGLATGSTPERLYR
                     IMRESDLDFSGMTSINLDEYMGLSEDNPQSYHYFMHKHLFDAKPFKKNYLLDGLADET
                     EECAQFEEIIAANPIDVQILGIGTNGHIGFNEPGTSFDSRTQKVALWQSTIDSNKRYF
                     EDEKDVPRFAYSMGIQSILSSRKIILMAFGKEKAAAVKAALEGPVTEELPASILQTHK
                     HVTVILDEEAAQMLTKK"
BASE COUNT          920 a          674 c          827 g          698 t
ORIGIN      
        1 aagccgctct cggaagaggg cggctttctg ctgtctaaaa gtgtctccaa tagataagtt
       61 aaagaaaatt attttcaata aacaataatt gaaaaaacta ttttcatgtg ttattctatc
      121 agcgaagggg aaaaggcgaa gtcaagaaat gcgcagtgcg cttttggcgg ttgcgcaaag
      181 caggaggagg caacaagtaa atgacgatta tcgaagagat ccaaaatcag tattctgctt
      241 tttccgcgaa cgagaaaaaa attgcggatt acattctgca gaacaaagca tccatcaaaa
      301 atatgaacat caaggatttg gcagcgaagg cggatacctc gacttccagc atcacccggt
      361 tctgtaaacg catcaacatc gatgggtttg tggacatgaa gattgcgctg cgttcattgg
      421 cgagcggtca tccttcgaaa gaaatgccga ccgtctatga cgatatttat ggctattata
      481 cacaagtcat cgctgaaacc aatcagatga tcgaggatca ggcaatcgaa acggttggtg
      541 attggatcaa aaacgcaaac aaaacagtca tctacggcat cggcagttcc ggtttcacgg
      601 ccgcggaatt ggcgcaacgc ttgatccgga tggggctgaa tgctacgggg gttacggatt
      661 cccacctgat gatcatcaac agttcgatca tcaaggaggg cgaattggtg atcgccattt
      721 caaattcggg agagacccct gaactgattg catctttgga gatcgcgaag aagaaaaaag
      781 cgaagatcat cgccctgacg agcttcaaaa acagttcgct gacgaaactg gcggatgtca
      841 cgtgcttcgg ttatcactcg cgtttcgtca acaacacggt gttcgtcaac acgcagttcg
      901 ggatgatgta cctgatcgat gtgatttcga ccttcctgtt gcaggaccat gatttccaat
      961 acaatatgga tctgacgaga gcaaatgtgc aaggttattc aaaaaaaaca gaatagcgcg
     1021 atgtgctgtt tgagtcatgc aagtggacag tgaaaaaagt tcacgatcaa agagggggat
     1081 acaaatgaaa agagtgatca tccatgcaga agtttatacc ggagaaacag tcatttttga
     1141 tggattcgtc cggtttgaaa aagaaatcag cgcagtcgga aagatgtcgg agtacgttcc
     1201 tgaatcaggc gaagaagtga ttgatgcagc cggaagcaga ctggtgcccg gattcattga
     1261 tgtccacagt catggcggat atggtgtgga caacatggac ggtgatgcgg aagacatcaa
     1321 tcggatgatt gcagcgatgc accgggaagg gatcaccagc tattttccga cgacgatgac
     1381 gcaatcttct gaaaatatcg agagggcatt gatcggcatc cgcaaggctt cagaaaaaaa
     1441 tccagtcatc caaggcatcc acctcgaagg gccattcatt tctgtcgtcc ataaaggggc
     1501 gcaaccggaa gaacacatca aactgccgga tgctgcctta atgcgcaaat ggcaggactt
     1561 gagcggcggc ttgatccgtt tggtaaccta tgcgccggag accactgatg cgacggcatt
     1621 cgaaaagtat tgtctcgata accgcatcgt gctttcggtc gggcattcgg atgctcttcg
     1681 cagccagctg agaaaatcca aggcagcgca tgtcacccat ctcttcaatg cgcaaagggg
     1741 actccatcat cgtgaaccgg gtgtgaccgg acatgcgttc ctggaggatg acatctatgc
     1801 ggaaatgatt gtcgatggcc tgcatatcga tccggaaatg gtcaagatcg ccttcaaaca
     1861 aaaaggcgca gaccgcatcg aattgattac cgatgccatg cgtgccaaag ggatgggcga
     1921 cggcatcagt gaattgggcg gacagaaagt catcgtcaaa gaaggacaag cccgtctgga
     1981 aagcggaaat ttggcaggaa gcgtgctggg atttcaggag gctttcagga acatcatcgc
     2041 cttcaccggc tgcagcatct tggatgcggt aaagatgagc tcagtcaatc aggcccgtga
     2101 attcgatttg gtgcgaaaag ggggcatcgc tcctggaaaa gatgccgata tggtcctgct
     2161 ttcggagtca tttgcggtcg agcggacaat cagttatgga cagacagtat acaaaaataa
     2221 cagaaagtag gttacagatc atggaaataa tcattaaagc gactcctgaa gaggcaagcc
     2281 gttacgcatt cgaaaaaatc cgcgaggaaa tggaaagagg tgcgaaagtg ctcgggttgg
     2341 caaccggcag cacgccggag aggctgtacc ggatcatgcg ggaaagtgat cttgattttt
     2401 cggggatgac atcgatcaat ctggacgaat atatgggtct ttccgaagac aatccgcaga
     2461 gttatcacta tttcatgcat aagcatttgt tcgatgcaaa accgttcaag aaaaactatc
     2521 tgctggacgg attggcggat gaaacggaag aatgcgcgca attcgaagaa atcatcgccg
     2581 cgaatccgat cgatgtgcag attctcggca tcggcacgaa cgggcatatc ggttttaacg
     2641 agccgggcac atcttttgat tcccggaccc agaaagtggc attatggcaa tccacgatcg
     2701 attccaacaa acgttacttt gaggatgaaa aggatgtccc gcggtttgcc tattcgatgg
     2761 ggatccaatc gatcctctct tcaagaaaga tcatcctgat ggctttcgga aaagaaaaag
     2821 ccgctgccgt caaagccgca ctggaaggtc cggtcacgga agagttgccg gcgagtatcc
     2881 tgcagaccca caagcatgtg actgtgatac tcgatgaaga agctgcacaa atgctcacaa
     2941 aaaaatagtg aatcagacaa tccgcagact actccccatg agtctccgga ttgtcgtttt
     3001 tgattaaaac gtttacaatg gaagcagaga tagcatgtaa agcatgtgat tcaggaatcc
     3061 aaggtggtga tggaaatgtt gactgtcgag acagccagaa catggttgag cctgttcgt
//
LOCUS       DAOMUJ010000190         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_8946_length_3105_cov_2.328525, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000190 DAOMUJ010000000
VERSION     DAOMUJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8946_length_3105_cov_2.328525"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1012
                     /locus_tag="PLT73_05620"
     CDS             44..1012
                     /locus_tag="PLT73_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRM19893.1"
                     /translation="MVNKVTHPILFQGDLKKQGPYFEGWYFKSVSADEKTVLSLIPGI
                     SLTAEDPHCFIQYSYVHVGEAPGKELRTGYIRYLLSAFQYNQTPFVLKVGNSVFSETL
                     VSVHLEEDDFRMEGILKLGPFQAIKRTPLSPSIMGFFAYIPNMECYHGVISMNHAVDG
                     TVAFGTKACDFTGGKGYLEKDWGTSFPEEYVWIQCNHFRDEGTSLFFSVAKIPYRGSS
                     FEGFICNFIVDGIEYRFATYNRSKFTLGLLTDDRIEIEVSHASASLTICAEVLHGGAL
                     VAPTRQGMDKLIKEGLSGIVTVDFTDQKTGKIYHDVGNVSGIEVVR"
     gene            1041..1271
                     /locus_tag="PLT73_05625"
     CDS             1041..1271
                     /locus_tag="PLT73_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HRM19894.1"
                     /translation="MSQNLFTYRIQTSGKQSLTNIDVYLEDAMTKSGVKDGILVVFCP
                     HTTAAITINDQRERGSGCAEGHELGLYGHVSE"
     gene            1237..1455
                     /locus_tag="PLT73_05630"
     CDS             1237..1455
                     /locus_tag="PLT73_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRM19895.1"
                     /translation="MNWGYTDTFPNKGSYRHSEGNSDAHIKSSLTGVSETIIVTEGRM
                     LLGTWQSIYFWDYDGPRNRKFHVKIIAG"
     gene            1512..2165
                     /locus_tag="PLT73_05635"
     CDS             1512..2165
                     /locus_tag="PLT73_05635"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRM19896.1"
                     /translation="MKLKQAVGECTMLEDIKKIIAEHKPEPIGEQRNFSVLIPLIRVD
                     GALHVLYEVRGAHISQPGDTSFPGGAVEPGESFEAAAIRETMEELNLKSENIKILGEM
                     DYIMNDRAIIHCFVGEITGVEKDDIRFNKEVQEIFTVPLQYFMDNRPTYYSSTVRLEH
                     PDDFPFELIPNGKDYKFKVGVHCVPFYHLEHHRLWGYTANLTDRFVNILEQNKQSSK"
     assembly_gap    2422..2438
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2537..2974)
                     /locus_tag="PLT73_05640"
     CDS             complement(2537..2974)
                     /locus_tag="PLT73_05640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRM19897.1"
                     /translation="MKNELKPYIHVAQYYETDQMKIIHHSNYIRWFEEARIDFLDQVG
                     ANYAKLEANGIVSPVLTVEAQYKSMVRYGDSVYVLPEIESYNGVKLSLAYRVIDVATG
                     ELRTTGKSQHCFLNEAGRPVSLKKVQPSVHGSFEKYRAHIFSI"
BASE COUNT          830 a          692 c          794 g          772 t
ORIGIN      
        1 gcatagtaaa gataagaatg catgcgaaag gaagcgctgc gctatggtaa acaaagtgac
       61 ccatccaata ctgtttcaag gcgatctgaa aaagcaagga ccctattttg aagggtggta
      121 tttcaagagt gtttcggctg atgaaaaaac cgtcctcagt ctgattccgg gaatatccct
      181 taccgctgaa gatccgcatt gtttcatcca atattcctat gtccacgtcg gagaagcgcc
      241 cggaaaagag ttgcggaccg gctacatccg ctacctcttg tctgcgtttc aatacaacca
      301 aacgccattt gtgcttaaag ttggaaatag cgtcttttcg gaaacgctcg tcagcgtcca
      361 tctggaagag gacgatttcc ggatggaagg catcctgaaa ttagggccgt tccaggcaat
      421 caaacggaca ccgctatcgc catccattat gggtttcttt gcctatatcc cgaacatgga
      481 atgctatcac ggcgttatca gcatgaatca tgccgttgac ggaacagtgg ctttcggaac
      541 caaagcttgt gattttacag gcggaaaagg ctaccttgaa aaggactggg gaacgtcttt
      601 cccggaggag tatgtctgga tccagtgcaa ccatttccgg gatgagggga cgagcctctt
      661 cttctcggtg gcgaaaattc cttatcgggg atcctcgttt gaagggttca tttgcaactt
      721 tatcgtggat gggatcgaat atcgttttgc gacctataac cgcagcaaat tcactttagg
      781 tttgttgacc gatgatcgca tcgagatcga agtttcacat gcatccgcat ccctcaccat
      841 ttgcgcggag gtgctccacg gcggggcctt ggtggcgccg accaggcagg gtatggacaa
      901 gttgatcaaa gaagggcttt ccggcatcgt tacagtcgat tttaccgatc agaaaactgg
      961 aaaaatttac cacgatgtcg gaaatgtttc cggcatcgaa gtagtacgtt gaacatttga
     1021 aagagtggga gggaaaggaa atgagtcaaa atttgttcac ttaccgtatc cagacgagcg
     1081 gaaagcaatc attgacgaac attgatgtct atctcgagga cgcaatgacg aaaagcggcg
     1141 tgaaggacgg catcttggtt gtcttctgtc cgcatacgac agctgccatc acgatcaacg
     1201 atcaacgaga acgcggatcc ggatgtgcag aaggacatga attggggcta tacggacacg
     1261 tttccgaata agggaagcta cagacacagc gaaggcaact ccgatgccca catcaaatcc
     1321 tctttgacgg gtgtcagcga aacgatcatt gtgacggaag gacggatgct gttggggact
     1381 tggcaaagca tctatttctg ggactatgat ggtccgcgta accgtaaatt tcacgtaaag
     1441 atcatcgctg gataaatgtt tgatttgcag cttgagcata ttgtttcgaa ttcgagataa
     1501 aaacctgtat aatgaaatta aaacaagcgg taggtgaatg cacaatgctg gaggatatca
     1561 aaaaaataat tgcggaacat aagccggagc cgatcgggga acagcgcaat ttttccgttc
     1621 tgatcccttt gataagagtg gacggagcgc ttcatgttct ttatgaagta cggggagcgc
     1681 atatatccca acctggggat acttccttcc cgggtggtgc agtggagccg ggagaaagct
     1741 ttgaagcggc ggcgatccgg gaaaccatgg aagagttgaa cctaaagagc gaaaacataa
     1801 aaattttggg ggagatggac tatatcatga atgatcgcgc aatcattcat tgctttgtcg
     1861 gggaaatcac cggtgtggaa aaggatgata tccgtttcaa taaagaggtt caggaaatct
     1921 tcacggtgcc gcttcagtat ttcatggaca atcggcctac ctattattct tcgacggtcc
     1981 ggctggagca tcccgatgat ttcccgtttg agttgattcc aaacggaaag gactacaaat
     2041 ttaaggtcgg tgtccactgt gtgcctttct accatcttga gcatcatcga ctgtggggat
     2101 ataccgcgaa cctcactgac cgcttcgtca acatactgga acaaaacaag caatcatcaa
     2161 aataagcatg aagaggccgg ctcgctaaga gccggcctct tcatgcttat tggcacataa
     2221 ttcgcagtta tctgccttct cggtgctctg ccggtgcgga tagtcgagag ttaaaacttc
     2281 tgtcaggtga ataactaccg gaaactacta aataccggaa ggatcgcccg tttgtcgggc
     2341 atcagtccag gacggtatac cgaagaaccg agagtgcgcg aatctctcga cagaatgatc
     2401 accgaaaacc gcaaaacacc cnnnnnnnnn nnnnnnnntc actcaaagtg gtccgaactg
     2461 tcgacttttc ggcattattg gccgttctta aaagccgaaa gcaagagttt aatttgaaaa
     2521 aataagtagg gctccctcag atgctgaaaa tatgtgcgcg atatttttcg aagcttccat
     2581 gcacgcttgg ttggactttc ttcaacgaaa cagggcgtcc agcctcgttc aggaagcaat
     2641 gctggctttt tccggtcgtg cgcagctcgc cggtcgcgac atcgatgacg cgataggcaa
     2701 gactcaattt gacgccgttg tacgactcga tttcaggcag gacgtagacg gaatcgccat
     2761 agcggaccat cgacttatat tgggcctcga ctgtcaggac cgggctgacg atgccgttcg
     2821 cttcgagttt cgcgtaattt gcaccgacct gatcaaggaa gtcgatgcgg gcttcctcga
     2881 accaacggat gtaattcgaa tggtggatga ttttcatctg atctgtttca tagtattggg
     2941 cgacgtggat atagggtttc aattcatttt tcaaaaaagt tcctccttgc ggcagttcat
     3001 tagggatatg aaagacttat ccctaatgaa ctgccttttt tcttgctggt aggttactgg
     3061 ttgtccgata cagcgcagtt atcggacaac cgccgaagca tgtgg
//
LOCUS       DAOMUJ010000191         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9109_length_3068_cov_2.124793, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000191 DAOMUJ010000000
VERSION     DAOMUJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9109_length_3068_cov_2.124793"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLT73_05645"
     CDS             complement(<1..368)
                     /locus_tag="PLT73_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM19898.1"
                     /translation="MELKKTLSTATLLLASTFVLAACGGADTETETESSAAASSEVVV
                     ESSSEATSEAAAELQDGTYTLVEKNFDTRGWKTEFSITVVDGKITESTFDNVNEAGVK
                     KSEDAEYQANMAEKVGVGPA"
     gene            complement(579..1559)
                     /locus_tag="PLT73_05650"
     CDS             complement(579..1559)
                     /locus_tag="PLT73_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013712061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM19899.1"
                     /translation="MKIHSMWDQYPLLQGELVATNELLKKNITLKNQAVREAILARLL
                     SGGKMLRPAYCLLFSYYSPQRDAERAQAIAAALELLHTATLMHDDVIDEAGTRRGQET
                     MNRAFGNQIAVYSGDYLFTVCFRLLSEYAGDAAVLKIDTNGMESILIGELNQMDMRYN
                     PDMRMRDYLRQIQGKTAQLFALSCYAGAYGSDEDEKLARLAYRIGHDIGMAFQVMDDI
                     LDYTQDEETLGKPALSDFRNGIYTAPVLYAMQVDRKAFAGYIAKGADVTAMELAEIHA
                     LVEKYGGNRAAQALAKKYTIKALKLIKKLPNHPAKETLTKLTEQLLYRNM"
     gene            complement(1595..2017)
                     /locus_tag="PLT73_05655"
     CDS             complement(1595..2017)
                     /locus_tag="PLT73_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRM19900.1"
                     /translation="MRKYLKMIRRGDLLIILFLMAASFLPLGVFSYHQANAESADHIA
                     VISVDGNVVKEFVLKDDGQTETYVFQDDYGHENVIVREGTQIRIDSADCDDQLCVRMG
                     AKDGIGETIVCLPNRVLVEVRGTQGATDEDEEALDIIS"
     gene            complement(2090..>3068)
                     /locus_tag="PLT73_05660"
     CDS             complement(2090..>3068)
                     /locus_tag="PLT73_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase FAD-containing subunit"
                     /protein_id="HRM19901.1"
                     /translation="YLAGDLVYYEEPDKNNAPVPQIVQSAEQTGHTAAANIIASIEGT
                     EKHEHKGTYQGFMISIGSRYGVAYLMDKIHLSGFFAMLVKHIVNLFYFMTIGSGYYFV
                     QYIYHEFFHIKEKRNIFRGHLSRLGNVLWALPLRVFYGSMWTWEALKKIFGLYGTTSW
                     FGDDVVLPFAWLKEATTGASEAVTETVSNPVFGLSYAYGEEPMMIFKEAPEWFNSIMK
                     IMIPNVEMALFFQKFMTIVELLIGLAIIAGLFTFLANAATIALVVSFSLSGMFYWVNM
                     WFIPVAIALMNGSGRAFGLDYYVIPWIQKKLDNWWYGKTKSIYRPGIGQ"
BASE COUNT          751 a          837 c          656 g          824 t
ORIGIN      
        1 tcagcaggac ctacgccaac tttttctgcc atgtttgctt ggtattctgc atcttcggat
       61 ttcttcacgc cggcttcgtt cacgttgtca aaagttgact ctgtgatttt gccatccact
      121 acagtgatcg agaattcagt tttccagcca cgtgtgtcga agttcttttc aaccaatgta
      181 tatgtgccgt cttgtaattc agctgccgct tccgaagtag cttcagaaga agactcaact
      241 acaacttctg atgaagctgc tgctgatgat tccgtttcag tttctgtgtc ggctccgccg
      301 caagctgcta atacgaaagt tgatgccagc aaaagagttg ccgtagataa cgttttcttt
      361 aattccataa tctatcccac cctgttcttt tttatcataa tggctagata tgtgatacca
      421 atcacaagcc ccctatatct atccacttac ttatcatacc atttgttaca tctttagcca
      481 aataaaattc atatatttaa caaaaaaccc attattttct aatgttttgt tttaaagcga
      541 cttgctatcc atccgtataa cacgtagcaa aagttttgct acatattgcg ataaagcaat
      601 tgctcagtta atttcgttag cgtttccttt gccggatggt tcggcaattt ttttatcaac
      661 ttcagcgctt ttatcgtgta cttcttggca agtgcctgcg cggcgcggtt cccgccgtat
      721 ttttccacca aagcatggat ttccgcgagc tccatggcgg tcacatccgc ccccttggcg
      781 atataaccag cgaaagcttt acggtcaacc tgcatcgcgt acagcacagg tgccgtgtag
      841 atgccgttac ggaagtcgct cagagccggt ttgcccaaag tctcttcatc ctgcgtgtag
      901 tccaagatat catccatgac ctggaaggcc atgccgatgt cgtgcccgat ccgataggcc
      961 agccgagcca acttctcatc ctcatccgat ccataggccc ctgcatagca gctcaaggcg
     1021 aacagttggg ccgtcttgcc ttggatctgg cgtaaataat cacgcatgcg catatccgga
     1081 ttgtagcgca tatccatctg attgagctca ccgatcagga tgctctccat cccattcgta
     1141 tcaattttta gcaccgctgc gtcacccgcg tattcggaca gcaagcggaa gcagaccgtg
     1201 aagagataat cccccgaata gacagcgatc tgattgccga atgcccggtt catcgtctcc
     1261 tggccgcgac gtgtgccggc ttcatcgatt acgtcatcat gcatcaacgt agccgtatgc
     1321 aacaactcca atgcagccgc gattgcctgc gcccgttccg catcccgttg cggggaatag
     1381 tacgaaaaca agaggcaata ggccggccgc agcattttcc cacctgaaag caggcgcgcg
     1441 agaatcgctt cccttaccgc ctggtttttg agcgttatat tttttttcaa cagctcattg
     1501 gtggcgacca attccccttg caacagcgga tactggtccc acatcgaatg aattttcata
     1561 ctgaactcct ttttgttctg cccttaagta attatcagga gatgatgtcg agggcctctt
     1621 catcttcatc ggttgctcct tgagtacccc gcacctccac cagtacgcgg ttcggcagac
     1681 acacgatcgt ctccccgatg ccatccttcg cgcccatgcg cacacataat tggtcgtcgc
     1741 aatcggcgga atcaatccgg atttgggttc cttcgcgcac aatcacgttc tcgtgcccat
     1801 agtcgtcctg gaaaacatac gtctccgttt ggccgtcatc cttcaagaca aattccttca
     1861 ccacgttgcc gtccacactg atgactgcaa tatgatcggc actctcagcg ttggcttgat
     1921 gatagctgaa gacccccaac ggcaggaagg aagccgccat caaaaaaaga atgatgagca
     1981 gatcgcccct gcggatcatt ttcaggtatt tcctcatttc cccatccctc tctttcgaaa
     2041 agaaaagggt taaggcaagt ctccccacct taaccctgtt agatcgttat tattgaccga
     2101 tacccggtct atagattgat tttgtcttac cataccacca gttgtcaagt ttcttctgga
     2161 tccaaggaat cacatagtag tccaacccga aggctctgcc tgaaccgttc atcaaagcga
     2221 tagccactgg aatgaaccac atgttcaccc aatagaacat gccggataag gagaacgaga
     2281 ccaccaatgc gatcgttgca gcattcgcca agaacgtaaa caagcccgcg atgatcgcca
     2341 aaccgatcag taactcgacg attgtcatga atttctggaa gaacaaagcc atttccacgt
     2401 tcgggatcat gattttcatg atgctgttga accactcagg tgcttctttg aagatcatca
     2461 tcggttcttc accgtatgca tagctcaaac cgaagacagg attgctgact gtttccgtta
     2521 ctgcttcgga tgcaccagtc gtcgcttcct tcagccaagc gaatggcaag acgacatcat
     2581 cgccgaacca agaagtcgta ccgtacaagc cgaagatctt cttgagcgct tcccatgtcc
     2641 acatgctgcc gtagaagacg cgtaacggca gtgcccagag tacattgccc aaacgcgaca
     2701 aatgcccgcg gaagatgttg cgtttttctt tgatatggaa gaattcatga tagatgtatt
     2761 gtacgaaata atagcccgat ccgattgtca tgaagtagaa caagttcacg atgtgcttca
     2821 cgagcatcgc gaagaaaccg ctcaaatgga ttttgtccat caagtaggcc acaccataac
     2881 gggaaccgat cgaaatcatg aacccttgat acgtgccctt gtgttcgtgc ttctcagtgc
     2941 cttcaataga agcaatgata ttggcggccg ctgtgtgtcc cgtctgttcg gcggattgca
     3001 cgatttgcgg taccggcgca ttgtttttgt caggctcttc gtagtagacc aaatcccccg
     3061 ccaagtaa
//
LOCUS       DAOMUJ010000192         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9127_length_3064_cov_1.732137, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000192 DAOMUJ010000000
VERSION     DAOMUJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_3064_cov_1.732137"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLT73_05665"
     CDS             complement(<1..436)
                     /locus_tag="PLT73_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM19902.1"
                     /translation="MENRDKQRILIIEDEKNLARFIELELKHEGYETEICYNGRAGLE
                     AAMNQDWDVILLDLMLPELNGIEVCRRLRPFKDTPIIIMTARDSVIDRVSGLDHGADD
                     YIVKPFAIEELLARVRALLRRIDIEEEQRKVKQTTVTYRDLTI"
     gene            complement(609..2045)
                     /gene="gndA"
                     /locus_tag="PLT73_05670"
     CDS             complement(609..2045)
                     /gene="gndA"
                     /locus_tag="PLT73_05670"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRM19903.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVSVFNRSGSKTEQVIA
                     ENPEKKLVPTYTIEEFVESLEKPRRIMMMVKAGEATDKTIQSLLPHLDKGDILIDGGN
                     TFYKDTIRRSKELENSGINFIGTGVSGGEEGALKGPSIMPGGQREAYDLVAPILEQIS
                     AKAPDGEPCVAYIGKDGAGHFVKMVHNGIEYGDMQLIAEAYDIMFKYLGMSVEEIAAV
                     FIEWNKGELDSFLIDITADILTKYDPATGKPMVEIILDRAGNKGTGKWTSQSALDLGV
                     PLPLITESVFARYISALKTERVAASEILTGPAAATEEGLDKAAFVESIRKALYFSKIM
                     SYAQGFAQLRVASEENDWDLNYGEIAKIWRAGCIIRAQFLQNITDAYEKNPELQNLLL
                     DDYFVEITKQYQGAVREVVATAVTAGVPVPTFSSAVAYFDSYRSAVLPANIIQAQRDY
                     FGAHTYERTDRAGAYHFEWDKEVEVPQD"
     gene            2229..2363
                     /locus_tag="PLT73_05675"
     CDS             2229..2363
                     /locus_tag="PLT73_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HRM19904.1"
                     /translation="MLTKKTGATSSKFNKFSKFIIWIMLLAMVGGTFLSALYYLFLNM
                     "
     gene            complement(2428..2829)
                     /locus_tag="PLT73_05680"
     CDS             complement(2428..2829)
                     /locus_tag="PLT73_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM19905.1"
                     /translation="MNLTGIDIFNIALWIFIIGYAIYQAYFYFQRKNAATTLTAEEFR
                     KDLRKAQLIDVRERAEFEAGHILGARNIAYSAFKQRYPEIRKDQPIYLYDQNAMLSGR
                     CAAILKKNGYKNIFILKGGYAGWDGKVKKGI"
BASE COUNT          792 a          746 c          598 g          928 t
ORIGIN      
        1 cgatcgtcaa atcacgataa gtgactgtcg tctgtttaac cttgcgctgc tcttcttcga
       61 tatcgatgcg acgcagcaaa gcgcgcaccc ttgccaaaag ttcttcgatg gcaaatggct
      121 tcacgatgta atcatccgct ccatgatcca aaccggatac gcggtcaatt acggaatcgc
      181 gggcagtcat gatgatgatt ggcgtatctt tgaacgggcg caagcgacgg caaacttcga
      241 taccattcag ttccggtaac atcagatcca ataggatgac atcccagtcc tggttcatgg
      301 cagcttcaag gccggctcta ccgttgtagc aaatttccgt ttcgtagcct tcgtgtttta
      361 actcaagctc gatgaaacgc gccagatttt tttcatcttc gataatcaat attctttgtt
      421 tgtctctatt ttccatagaa ttctccccta atgtctcatt tgaataataa caacatgcca
      481 ttacaatttc attttatttt aacacactgt cgggaatttt tcctgattaa atgatcctga
      541 tagtaaaatg tcatccgaag cacagctccg gatgacgtaa ttgttggcca gaatggcgtt
      601 ttatcccttt agtcttgtgg aacttccact tctttatccc actcgaagtg ataagcaccc
      661 gctctatcgg tgcgctcgta agtatgcgct ccaaagtaat cgcgttgcgc ttgaatgatg
      721 ttcgctggca agacagccga acggtaagaa tcgaaataag ctaccgcaga tgagaatgtc
      781 ggtaccggaa ctcctgcagt cacagccgtt gcaaccactt cacgtacagc tccttggtac
      841 tgtttcgtga tctctacgaa gtagtcatcc aacaacaagt tctgcaattc aggatttttc
      901 tcgtaagcat ccgtgatgtt ttgcaggaat tgcgcacgga tgatacagcc tgcgcgccaa
      961 attttagcga tttcgccgta attcagatcc caatcatttt cttcgctggc aacgcgcaat
     1021 tgagcgaagc cttgcgcata gctcataatt ttgctgaagt atagcgcttt gcggatactc
     1081 tcaacaaacg ccgctttgtc caaaccttct tcagttgcag cggcaggtcc ggtcagaatt
     1141 tcgctggcag cgacacgttc tgtcttcaaa gcggagatgt agcgggcaaa aacggattcg
     1201 gtgatcaatg gcaacggcac acccaagtcc aaagcgcttt gggaagtcca tttacccgtg
     1261 cctttattgc cggcgcggtc caagataatc tcaaccattg gcttgcctgt tgcagggtcg
     1321 tatttagtca agatatcagc agtgatgtcg atcaggaagc tgtccagctc gcctttgttc
     1381 cattcgatga agactgccgc gatttcttcc actgacatgc caagatattt gaacatgatg
     1441 tcgtaggctt ccgcaatcaa ttgcatatct ccgtactcga ttccgttatg gaccatctta
     1501 acgaaatgtc cggcaccatc tttgccgata tacgctacac acggctcacc atctggagct
     1561 tttgcggaaa tttgttctaa aatcggtgca acaagatcat aggcttcacg ttgtcctcct
     1621 ggcatgattg aaggaccttt caaggcgcct tcttcaccgc cggaaacacc cgttccgatg
     1681 aaattgatgc ctgaattttc caactccttg ctgcggcgga ttgtatcttt gtagaaagta
     1741 ttccctccgt cgatcaggat atctccttta tccaaatgag gcagcaacga ttgaatcgtt
     1801 ttgtcggttg cctcccctgc ctttaccatc atcatgattc ttcttggttt ttccaaagat
     1861 tcaacgaact cttcgattgt ataagtagga accagttttt tttctggatt ttccgcgatc
     1921 acttgctcag tctttgagcc ggagcggttg aatacagata cagaataacc tctgctttca
     1981 atgttcaatg ctagattttt gcccattacg gccatcccga caacgccaat ttgctgtttt
     2041 gacataaata aattctccct tcatgataaa cacatcattc tataatctta atcctaacaa
     2101 aaatttactt aaatggaaag caatcaattg aaaataatgg ctttcttttc ttcccctcgg
     2161 ttctcaacat ttacttttac attttaatgt tgtaaactat gccatgtaaa aaacaaaagg
     2221 tgggaataat gttgacaaag aaaacgggtg ctacctcttc aaaattcaat aaattcagca
     2281 aattcatcat ttggatcatg ttacttgcca tggtcggagg aactttcctt tccgctctgt
     2341 actatttgtt cctaaatatg taggaaaaaa caatcaaaaa ggttcccgga atccattagc
     2401 ggattccggg aacctttttc aatcgactca gatgcctttt ttgacttttc catcccagcc
     2461 ggcatagccg cctttcagga tgaaaatatt tttgtagcca ttttttttca aaattgccgc
     2521 acaacgtccg ctcaacatgg cattttggtc atacagatag atcggctgat ctttgcggat
     2581 ttctggatag cgttgcttga aggcggaata agcgatattt ctcgctccca aaatatggcc
     2641 tgcttcgaac tccgctcttt cgcggacatc aatcaattgc gctttccgca gatcttttct
     2701 gaactcctct gcagtcagtg ttgtcgctgc attctttctt tggaaataaa aataagcttg
     2761 ataaatggca tatccgatga tgaatatcca tagtgcgata ttaaaaatat caattcctgt
     2821 taaattcaaa ataaatccat cctttcgata atcttgctct catattttac acttgtgcaa
     2881 ggcgcattgc tacagttttg acgacttttt ttctattccg acaattgtat ccagccattg
     2941 cgttccacat cgtacagata ctcataacgc tccccataaa gcacgtagct cccttctggc
     3001 gaaacagaaa tcggagccat tcgttcgact tcagtcagca ccttcatagc gttttcggga
     3061 aaat
//
LOCUS       DAOMUJ010000193         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9225_length_3042_cov_2.118514, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000193 DAOMUJ010000000
VERSION     DAOMUJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9225_length_3042_cov_2.118514"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLT73_05685"
     CDS             <1..444
                     /locus_tag="PLT73_05685"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM19906.1"
                     /translation="SVAILRNDGKIKDSARNLLASRQGVVHIVSEDLVEEMNRTAAQL
                     PDTESEIDLTELTLTASHTVTVPAIKEAKIRMETTVHQYVPLTDADGKVLTDLFILRV
                     EDFLFDETVFDHEKQYVWADKLAPVARLAGNDYTKLGEIFSLERP"
     gene            complement(548..1057)
                     /locus_tag="PLT73_05690"
     CDS             complement(548..1057)
                     /locus_tag="PLT73_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM19907.1"
                     /translation="MFGFFKKVDKKETLPVEKELLYSPANGIVVPVSDVADPVFSQKM
                     MGDGFAVIPTDGNIYSPAKGKVLSVFPTKHAVGILLDSGLELLLHMGLDTVELNGKPF
                     EVFVKEGQALTADTLIAKVDLAQLQEAGKDSAMVVVITNMDKVKSFSLDVTGEAAVKA
                     EIGSVLPKE"
     gene            1278..2065
                     /locus_tag="PLT73_05695"
                     /pseudo
     CDS             1278..2065
                     /locus_tag="PLT73_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
     gene            2325..>3042
                     /locus_tag="PLT73_05700"
     CDS             2325..>3042
                     /locus_tag="PLT73_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM19908.1"
                     /translation="MKDLLKNKAMIATTISGILIILGIVLQWQNFDTAAAIIFVLSFI
                     IGGYKQAKEGFIDTVENKHLNVDILMVLAAVGASLIGYWMEGALLIFIFSLSGSLEEY
                     ATDKSTQAITALMNIVPEIAKRINPDGSIEDVAVKDLKVGDTLMVPKGASIPIDGTIV
                     SGLGLIDEAAISGESVPVEKTVGDDIFGSTINLSEALTMTVSKESKDTLFAKIIRMVE
                     EAQKTPSKTASFINRIENTYV"
BASE COUNT          828 a          697 c          663 g          854 t
ORIGIN      
        1 agcgtggcca ttctgaggaa tgacgggaaa atcaaggatt ccgccagaaa cctgctggct
       61 tcccgacaag gagtcgtcca catcgtcagc gaagacttgg tggaggagat gaacaggacg
      121 gccgctcagc taccggacac agagagcgaa atcgatctga cggaattgac gttgaccgcc
      181 agccacactg tcactgtacc cgcgatcaaa gaagcgaaaa tccgcatgga aaccacggtg
      241 catcaatatg tgcctctgac ggatgctgat ggcaaagtgc tgacggatct gttcatcttg
      301 cgcgtcgaag atttcctttt cgatgaaact gtcttcgatc acgagaaaca atacgtttgg
      361 gccgacaaat tggcgcctgt cgcaaggctg gccggcaatg actacacaaa gctcggagag
      421 attttttcgt tggaacgtcc ttaacgggct gcgctccatt tagccaaggc caaagcatag
      481 ggaggcgata gcgaagcaaa aagctccatc cggctagccg gatgaagctt tttttaaatt
      541 cattctgtta ttctttcggc aggacgcttc cgatttctgc ttttacggca gcctctccgg
      601 tcacatccag gctgaaactt ttgactttat ccatatttgt gatcacaacg accatcgcag
      661 aatctttgcc cgcttcttgc agttgtgcca gatcaacttt agcaatgagt gtatcggcag
      721 tcaaagcctg gccttctttc acaaagactt cgaatggttt cccgtttaac tcaaccgtgt
      781 ccaaacccat atgcaacaaa agttccaatc cgctgtccaa caggatgcct accgcatgct
      841 ttgtcgggaa tacgctcaat acttttcctt ttgctggaga ataaatgttt ccgtcagtag
      901 gaatgaccgc aaacccgtca cccatcattt tttgggaaaa gaccggatct gcaacatccg
      961 aaactggaac cacaatacca tttgctggtg aatataagag ttctttctca accggtagtg
     1021 tttccttctt atcaaccttc ttaaaaaaac caaacatgct atcgcctctt tcttatttaa
     1081 atgagcttat gctaaaatta gtacacttca ttttaacata aatcgttcgg taatgtgtat
     1141 ttttttgagc accgcttaca tcgaaaaaga aataaatatc gttaagcaga atacttgaaa
     1201 atagttggct aatctttttc atttcgctat actagatgat ggagcatcaa aatattgggc
     1261 aacagaaagg atattatatg gaatttatgg aaatactgaa agcaatcatc ctcggaatcg
     1321 tcgagggaat aaccgaatgg ctgcccatca gcagcacggg tcatatgatc cttgtagaag
     1381 aattcatcca gttgaacctc tcgcaggcat tcaaagagat gttcttctat gtgatccaat
     1441 taggagctat cttggctgtg gtcgtcattt atttccacaa actgaatccc ttctctccaa
     1501 gcaaaacgaa ggcagagaag aaccagactt gggatatctg gaaaaaagtg atcatcggtt
     1561 gtatcccagc aggcgtaatc gggttacctt tgaacgattg ggcggatgaa aatctcttga
     1621 acgcgacggt cgtagctttg gcactgatca tctacgggat cttgttcatc gtgatcgaaa
     1681 acaggcacaa ggatcgcgaa ccgagcatta ccgatttcag ccaattaact tatatgaagg
     1741 cattccgggt acatcgcgct ccggttcttc catcctgggg agcatcgtat tgggtacttc
     1801 acgcttcatc gcgacagaat tctccttctt catgagcatt ccgatcatgt tcggaatcag
     1861 ttttttgaag atcatcaaat tcggcttcaa ctttaccgga cttgaattcg ccgttctttt
     1921 atcaggcatg cttaccgctt tcctcgtttc gatcgttgcc atcaagttct tgatcggtta
     1981 tctgaaacgc aatgacttca aggcattcgg ttggtatcgg attgtccttg gtgccatcgt
     2041 cctgatctat tttatgtttg tctgaaaaat gagtttcgcc atcctttttg ggatggcttt
     2101 ttattttgtc atccagaaca ttcatactcc ttattaacaa ttattataat ctgtattttt
     2161 ttgttaacct tctggtttta ttgctgtgcg taattttcta ccgcttcaaa ttgtgtctga
     2221 tatatggaca ttgagaatga gattcatttc cgtttgatta taatgattat aatctggatt
     2281 tctttagtgc tatcatgaag gcatatagga aggagtgtca aatcatgaaa gatttattga
     2341 agaataaagc gatgatcgca accacgatca gtggcattct catcattctt ggcattgtgt
     2401 tgcaatggca aaactttgat acagctgcag ccatcatttt cgtgttatcg ttcatcatag
     2461 gcggctataa acaagcaaaa gaaggattca tcgatacagt tgaaaataaa cacctgaacg
     2521 tcgacatatt gatggtgctt gcagcagttg gcgcctcttt gatcggctac tggatggaag
     2581 gcgcattgct catctttatc ttctcgttat ccggttcact ggaggaatac gcgaccgata
     2641 aaagtaccca agcgatcaca gcgttgatga acattgtccc tgaaatcgca aaacgcatca
     2701 acccggatgg ctcgatcgaa gacgttgcag tcaaggatct gaaagtcggc gatacattga
     2761 tggtccctaa aggcgccagc atccccatcg acggcacgat cgtgtcgggt ctaggcctga
     2821 tcgatgaagc cgccatttcc ggtgaatccg tccctgtcga aaagacggtc ggcgatgata
     2881 tcttcggcag cacgatcaac ttgagtgaag ctctgaccat gaccgtctcg aaagaatcca
     2941 aagatacttt gttcgccaaa atcatccgca tggtggaaga agcgcaaaag accccttcga
     3001 agacagcgag cttcatcaac cgcatcgaaa acacctatgt ta
//
LOCUS       DAOMUJ010000194         3039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9235_length_3039_cov_1.977212, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000194 DAOMUJ010000000
VERSION     DAOMUJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9235_length_3039_cov_1.977212"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLT73_05705"
     CDS             <1..715
                     /locus_tag="PLT73_05705"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRM19909.1"
                     /translation="NIMSYNELTKWIESGQTLIPNFLLQNYKRIGLTDTEFIFVLQLK
                     AYIDKNIPFPNLALIAENMGIAEKNVFSLLHNLMQKQMILMETKNDLAGKVEDSYSLD
                     PIYQRLFQLMERSEKKQPIQQQEKNIMTMFEQEFGRNLSPIEMQTIASWIDMDHYPLE
                     LIESALKEAVLNRVYSLKYIDRILLAWEKKNIKSVKDLVQQQSTRPQSNQDKFDEKAR
                     TDAPMEVPLYNWLDTDADA"
     gene            728..1375
                     /gene="nth"
                     /locus_tag="PLT73_05710"
     CDS             728..1375
                     /gene="nth"
                     /locus_tag="PLT73_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRM19910.1"
                     /translation="MLSKRKTVEALDEMAALFPNAQCELLHRNVFQLLIATLLSAQAT
                     DVSVNKVTPSLFDRFPTPEAFVAAPLESIMQEIRTIGLYRTKAANIQKCCKMLLEDFG
                     GEVPRTLEELTKLPGVGRKTANVVMSVGFHIPAIAVDTHVERISKRLRIAPQKASVLE
                     VEELLMKKIPKERWSDAHHQMIFFGRYHCTARNPKCEVCPLLSMCQEGQKRLGLK"
     gene            complement(1454..>3039)
                     /locus_tag="PLT73_05715"
     CDS             complement(1454..>3039)
                     /locus_tag="PLT73_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM19911.1"
                     /translation="YEGGLTITTNLDMDAQHRLYDIVNTYDYIEFPDDLLQTGVSMVD
                     VNTGAIQAVIGGRNQNVQLGLNRASTLERSIGSTMKPLSVYGPAIEYLDYSTGTLVVD
                     EAYSYSDGSEINNYDNKYKGDQTVREALVDSRNIPALKTFQEVGADNAFSFLQKLGIT
                     ITNDGQEYLVESNAIGGEATPIELSAAYAAFANGGTYYEPYTVQSVTTAEGEEFTFEP
                     NGTEAMKESTAYMITDMLKDVITEGTAKNAQISGLPQAGKTGTTNYTDEELAAVGGTN
                     VPYAAPDAWFAGYSTSYSISVWVGYDNSKEYGNFLDYETQSLTRDIYRELMSYVSENT
                     DYADWTLPTSVSQVSIEKYTDPIMKPGPNTPSNLIATELFVKGTEPTAISKKYGVTLL
                     APSGLKASYNADKDELTIEWDKYSASGIDSNAQAQYTVTAGGASEATTDTKVVIANPD
                     KGTITISLIVKVGSSTSPASSIQLTIADPAQESSEEDDEDEDKASSDSSSSSSSSSSA
                     ENDQASSDAQDDEETNQNP"
BASE COUNT          753 a          705 c          783 g          798 t
ORIGIN      
        1 caatataatg tcatacaacg aattaaccaa atggatcgaa tctggccaga cgctgattcc
       61 aaattttttg ctgcagaatt acaaaaggat cgggcttacc gatacagaat tcatttttgt
      121 gctccagctg aaagcttaca tcgataagaa catcccgttt ccgaatctgg cgttgattgc
      181 tgaaaatatg ggtattgccg aaaagaatgt cttttccttg ctgcataatc tgatgcagaa
      241 gcaaatgatt ttgatggaga cgaaaaatga tctcgccggc aaagtggagg actcctattc
      301 cttggatccg atctatcaac ggctatttca attgatggaa cgttccgaga aaaagcagcc
      361 catccagcag caggagaaga atatcatgac catgttcgag caggaattcg ggcggaactt
      421 atcgcccatc gagatgcaga ccattgcaag ctggatcgat atggaccact atccgctcga
      481 attgatcgaa tcagctctaa aagaagcggt attgaatcgc gtttacagcc tgaaatacat
      541 cgaccgcatt ctattggctt gggagaagaa aaacatcaaa agcgtaaaag atttggtcca
      601 acagcaatcg acgcggcctc aaagcaatca ggataagttt gatgaaaaag cgagaacgga
      661 tgcaccgatg gaagtgccgt tgtacaattg gctggacact gacgcggacg catagatggg
      721 gtgaaatatg ttatcgaaaa gaaagacggt agaagcgctg gatgaaatgg cggcgctgtt
      781 cccgaatgcg caatgtgaat tgttgcaccg gaacgtgttt cagctgttga ttgcgacact
      841 cctcagcgcc caggcaacag atgtttccgt caacaaagtt acgccaagcc tgttcgaccg
      901 cttccccacg cccgaggctt tcgttgcggc cccgctcgag tcgatcatgc aagaaatcag
      961 gacaatcggt ttataccgga ccaaagctgc caatatccaa aaatgctgca aaatgctgct
     1021 ggaggacttc ggcggcgaag tgccgcgcac gcttgaggaa ctgaccaagt tgcccggagt
     1081 ggggcgcaaa acagcaaatg tcgtcatgag tgtcggattc catatcccag caatcgcggt
     1141 ggatacccat gtggaacgga tatcgaaacg tctgcgcata gctcctcaga aagcatcggt
     1201 tttggaagtg gaagagttac tgatgaagaa gattccgaag gaacgctggt ctgatgccca
     1261 tcaccagatg attttcttcg gcaggtacca ttgcacagcc cgaaatccta agtgtgaagt
     1321 ttgccccctg ctttcgatgt gccaggaagg gcagaagcgt ttgggtttga aatgaaacgg
     1381 atccgagtag ctttcggcat ccggaacaaa aaaacagcca gctcctttat caatcgggag
     1441 ctggctgttt tttttatgga ttctggtttg tttcttcgtc atcttgtgca tcggaggatg
     1501 cttgatcgtt ttccgctgaa gagctggatg atgaagatga tgaggagtcg cttgatgcct
     1561 tgtcttcatc ttcgtcatcc tcttcggatg attcttgcgc cggatcggcg atggtcaact
     1621 ggatggaaga agccggtgag gtgcttgaac cgactttaac aatcagggaa atcgtaattg
     1681 tgcctttatc aggattagca atgacgactt tggtatctgt tgtcgcttcg gaagcaccgc
     1741 ctgccgtcac tgtgtattgt gcttgtgcat tggaatcgat gccgctggct gagtatttat
     1801 cccattctat ggtcagttcg tctttatctg cattgtagct ggctttcaag ccggatggcg
     1861 ccaacaatgt cacgccatat ttcttggaaa tggcagtagg ttctgttcct tttacgaaaa
     1921 gctctgtggc aatcaggttg ctcggcgtat ttggaccggg cttcatgatc ggatccgtgt
     1981 atttttcgat cgagacttga ctgactgatg tgggcagcgt ccaatcggca taatcggtgt
     2041 tctcggatac gtaggacatc aactcacgat aaatatcacg cgtcagactt tgcgtctcgt
     2101 aatccaaaaa gtttccgtat tccttggaat tgtcataacc gacccaaacc gaaatggagt
     2161 agcttgtaga atagccggca aaccaagcat ccggagcagc ataaggcacg tttgtcccgc
     2221 cgactgcggc caactcctca tctgtgtagt tggttgtccc tgttttgccg gcctgaggca
     2281 aaccggatat ctgggcgttc tttgctgtcc cttcagtgat gacgtccttc agcatatcgg
     2341 tgatcatgta ggctgtggac tctttcatcg cctccgttcc gttcggttcg aaggtgaatt
     2401 cttctccttc cgcagtcgtt acggattgaa ccgtatatgg ctcgtagtat gtgcctccgt
     2461 tggcgaaggc tgcataagcc gcggaaagct caatcggtgt cgcttcacca ccgatggcat
     2521 tggattcgac caaatactct tggccatcgt tcgtgatcgt aatgcctaat ttctgcagaa
     2581 aagaaaaggc attatccgca ccgacttcct ggaaagtctt cagtgctggg atgttgcgcg
     2641 aatcaaccaa tgcttcgcgg acagtctgat cacctttgta cttgttgtcg tagttgttga
     2701 tttcactgcc gtccgagtaa ctatatgctt catcaacgac caatgtaccg gtagagtaat
     2761 ccaggtattc gatggccggt ccgtagacag ataatggttt catcgtcgaa ccgatactgc
     2821 gttcgagggt ggaggcgcga ttgagcccta gctgcacatt ctggttgcgt ccgccgatga
     2881 ctgcctggat ggcgccggtg ttgacatcta ccattgaaac gccggtctgc agcagatcat
     2941 ccgggaattc gatgtaatcg taggtgttca cgatatcgta caaacgatgc tgggcatcca
     3001 tatcgaggtt ggttgtgatt gtcagtccgc cttcgtaga
//
LOCUS       DAOMUJ010000195         3039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9238_length_3039_cov_1.823056, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000195 DAOMUJ010000000
VERSION     DAOMUJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9238_length_3039_cov_1.823056"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLT73_05720"
     CDS             complement(<1..417)
                     /locus_tag="PLT73_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRM19912.1"
                     /translation="MSQAMMTVVSQTEIAKDIFELRLKGALVNEMGAPDQFLHIRVPG
                     DELLLRRPISIAAIDADKEECVIIYRTEGKGTTIISKLTAGDELDILGPLGNGYPIDM
                     LAAGQNALLIGGGIGVPPLYELAKQLHAKGVNIVSCF"
     gene            complement(540..>995)
                     /gene="pyrR"
                     /locus_tag="PLT73_05725"
     CDS             complement(540..>995)
                     /gene="pyrR"
                     /locus_tag="PLT73_05725"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014524830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRM19913.1"
                     /translation="DGTKNLILVGIKTRGIYLAQRIAERIKDFEGIEVPVGELDITLY
                     RDDVHRDTNENPVLHETNIPFSVAGKHVVLVDDVLFTARTIRAGMDAVMDLGRAKRIT
                     VAVLIDRGHRELPIKADYVGKNIPTSQAEQISVRLDEVDGIEKVTLIKA"
     assembly_gap    996..1005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1245..2150)
                     /locus_tag="PLT73_05730"
     CDS             complement(1245..2150)
                     /locus_tag="PLT73_05730"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM19914.1"
                     /translation="MTEKIVFNIDGQTGRIDKILADFMTGTSRSQIQAWIKDGNLMVN
                     DKAVKSNYKVQDGDLLELTIPEPEQINVTPEDIALDVIYEDDALLVVNKPSGMVVHPS
                     KGHLSGTLVNGLLFHVNSLSDGTANIRPGIVHRIDMDTSGLLVVAKTNEAHQKLAEQL
                     ENHTMERAYLALVHGVIPHEDGTIDAPIARMMVDRKKRTVADGGKKAVTHFEVIERFR
                     DFTLLKLRLETGRTHQIRVHMKYIGHPLAGDPMYGPAETLGGHGQYLHAATLGFIHPK
                     TGEKMHFEAPLPDFYSEKLTELRKN"
     gene            complement(2163..2639)
                     /gene="lspA"
                     /locus_tag="PLT73_05735"
     CDS             complement(2163..2639)
                     /gene="lspA"
                     /locus_tag="PLT73_05735"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRM19915.1"
                     /translation="MIIYYILAILLLVLDQWSKLAIVNNFALHEVKEVLPGILSLFYI
                     QNEGAAWGIFEGRMFFFFIITVVVVGAMVYNAHKLGFDKKIVGISYAFLLSGAIGNFI
                     DRMRLGYVVDMFRLDFIDFPIFNVADVCLTIGVILMMIYILFMEEEEKVSTKNKLK"
BASE COUNT          782 a          748 c          633 g          866 t
ORIGIN      
        1 gaagcagctg acaatgttga cacctttcgc gtgcaattgt ttcgccaatt catagagcgg
       61 aggaacaccg atgccgcccc caatcaataa agcgttttga cctgctgcga gcatatcgat
      121 cggataacca ttacccaatg gccccaagat gtccaattca tctcctgctg taagcttact
      181 gatgatggta gtgcctttcc cttcggtacg atagatgatg acacactctt ctttatcggc
      241 atcgattgcg gcgatgctga tggggcgtct caaaagcagt tcatccccag ggacccggat
      301 atgcaagaac tgatccggtg ctcccatctc attcactaat gcccctttca ggcgcaattc
      361 gaaaatatct tttgcgattt ctgtctgact gacgactgtc atcatagctt gactcataat
      421 tattttcctc cttaattttg acaaaataag gtgatctaaa aaaatgcaat ggagcaagct
      481 tcaaagcaaa ttcttctgag tctctcagga cacagttaaa gaatcaccaa tctatttgtt
      541 tacgccttga ttaatgttac tttttcaatg ccatccactt catccagacg gacggaaatc
      601 tgttccgctt gggatgtggg aatgtttttg ccgacgtaat ccgccttgat cggcagttcc
      661 cggtggcctc tgtcgatcag gaccgcaact gtgatccgtt tcgctctccc cagatccatc
      721 accgcatcca tgccggcccg gatcgttcgg gcggtgaaca ggacatcatc caccaacacg
      781 acatgcttgc cggcaaccga aaacggaatg ttcgtttcgt gcagcaccgg gttttcattc
      841 gtgtctcggt gtacatcatc gcggtacagc gtgatgtcca actcgccgac tggaacctcg
      901 atgccttcaa aatctttgat ccgttcagcg atgcgctggg ccaggtagat gccgcgcgtc
      961 ttgatgccaa ccaaaatcag gttctttgta ccatcnnnnn nnnnnatgcg tgatgcgggt
     1021 cagcgcgcgt ttcatcgtct gagcatccat gatttcgatt tcttctgcca tctccactac
     1081 tccttcgtcg tcaataataa aaaaaatacc cttatgcatg tgggcataag ggtacagtca
     1141 cgtgacgcta tacaaaatgt gatgcacctt gtagcctctc tggactattt taaaggttcc
     1201 ttcaatatta ctgtaatgat aaccaataga gccgtttctg tcaatcagtt tttgcgcagt
     1261 tcagttaatt tttcgctata gaagtcgggc aatggcgctt cgaagtgcat tttttcgccc
     1321 gtcttcggat ggatgaagcc taaagttgcc gcatgcaaat attggccgtg accaccgagc
     1381 gtttcagctg gtccgtacat aggatctccg gctaacggat ggccaatgta tttcatgtgg
     1441 acgcggatct gatgcgtccg gccggtttca aggcgcagct tcagtaaggt gaaatcacgg
     1501 aaacgctcaa tgacctcaaa atgcgtcact gcttttttac caccgtcagc tacggtgcgc
     1561 ttcttgcggt cgaccatcat ccgggcaatc ggcgcatcga tggtaccatc ctcatgcgga
     1621 atgacgccat ggaccaaagc aagataggcc ctttccatcg tgtggttctc aagttgctcc
     1681 gccaattttt ggtgcgcttc gttcgtctta gctacgacca ataagccgga tgtatccata
     1741 tcgatgcggt gcacaatgcc aggacggatg ttggccgtac catcggatag cgaatttacg
     1801 tggaataaca agccgttgac caaggtgcct gaaagatgcc ctttggatgg atgcacaacc
     1861 attcctgacg gcttattgac caccaataac gcatcgtctt cgtagatgac atccaatgca
     1921 atgtcttcgg gcgtgacgtt gatttgttct ggttccggaa tcgtcaattc cagaagatcc
     1981 ccatcctgga ccttataatt tgatttgacg gctttgtcgt tcaccatcaa gttgccgtct
     2041 ttgatccatg cctgaatttg cgaacggctc gtaccggtca tgaaatccgc caagatctta
     2101 tcgattctgc cggtttgacc atcaatatta aaaacgattt tttctgtcat ttgaatccca
     2161 ctttacttta gcttattttt tgtacttacc ttttcttcct cttccatgaa aaggatatag
     2221 atcatcatca ggatgacgcc tatcgtcaaa cagacatccg ccacattaaa aatcggaaaa
     2281 tcgatgaagt ccaagcggaa catatcgaca acataaccca accgcatccg gtcgatgaaa
     2341 tttccgatgg caccgctcaa aagaaaagcg taactgatgc ccacgatttt tttgtcgaac
     2401 cccagtttat gcgcattata aaccatggca cccacaacaa ctactgtgat gatgaagaaa
     2461 aagaacattc ttccttcaaa aatcccccag gcagcacctt cgttctggat atagaacaag
     2521 gacaagatac ccggtaatac ttcctttact tcatgcaatg caaaattatt tacgatggca
     2581 agtttgctcc actggtccag gaccaacagc aagatggcca atatataata gataatcatc
     2641 cgatagcctc acttattctg aaattaacgt cactacgtat cttttacctc tatctattgt
     2701 aaactaattt ttcgaattgt atagtcattg aatccagcct ttccgtttgt tttttaagcc
     2761 agcatacttg ctgcacccac ttttttaatg aattgctttt taatgaattg ctttttaatg
     2821 aattcttaat attatttcga agatattcca gactgccttg ataaaggaac gccattttcg
     2881 gtataatgaa gcttgtattg ttatatatca cgtgataaag gagcttgttt atgttgagag
     2941 gaaagaagat tgcattagtt tttgccggtg cactggtatt gtcagcttgc gcagaccaag
     3001 tcaaggaagc gacagagaca gtcaaccagc ttaaaaccg
//
LOCUS       DAOMUJ010000196         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9249_length_3037_cov_2.441985, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000196 DAOMUJ010000000
VERSION     DAOMUJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_3037_cov_2.441985"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..357
                     /locus_tag="PLT73_05740"
     CDS             28..357
                     /locus_tag="PLT73_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRM19916.1"
                     /translation="MYTGLASQYKEELEEKLMNVMDTEIGLDIVNLGLIYGIDLDDEG
                     VCNIRLTFTGAGCSCSEHILKGVHEQLDPLGFITEVKIKIVWSPAWVLDRITRYGRIS
                     LGINPGR"
     gene            577..1515
                     /locus_tag="PLT73_05745"
     CDS             577..1515
                     /locus_tag="PLT73_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HRM19917.1"
                     /translation="MLKLMRRVPGGTLLIPMFLSALLNTAFPEGLYVGSISQAFFSAQ
                     GTNYIVGFLCFVSAMGLDVKSLAQIMKKQGMLLVIKFMIAYVLGFLMIKYLGGGTILG
                     LSSLAIIVVLTSTNPALYMALVKDLSEKDDALAFGLAGVFCVPAVPLLVYSLSSGTDI
                     VWTPIISVVIPLLAGMIIGNLDHEFRDFCLPAIGIIMPFFGWVLGDSINLFQAAKAGL
                     AGIILSVLFYIILLPTLYFTETKLLKANGLSAIALTSVAGVSLAAPSVIAQAYPELEP
                     IVPLVVSQIAMAVVITSVLTPMIANKIAKKKNLVKH"
     gene            1531..2703
                     /locus_tag="PLT73_05750"
     CDS             1531..2703
                     /locus_tag="PLT73_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM19918.1"
                     /translation="MNKYYERAYALEEQLVKDRRTLHQNPEIGMELPNTRAYVRNRLE
                     EIGLEVKEVGKMGLSAVIEGEKPGKTILLRADMDALPMKEMNELEYKATNNMAHTCGH
                     DLHTAMLVTAAQILLENKADIHGRVKLMFQPGEEIFAGAKDMIEAGILENPKPDVAFA
                     MHTGLNQGVGSFEYYKGHTSTSCDNFKITITGKGAHGAYPHTSIDPINAGVIIYQEFG
                     ELISREVSPLSVATLTFGMFSGGSNSNIIPEVVEMQGTLRTYDDEVREYVKGRITDIL
                     AGIEKTTRAKIDFEIFASVPSLYNDPDLTEEIAEILETGNPAFKGYPDLRIMASEDMA
                     VVSRHLPTTYIMLNCKVEGNNFSHHNPGVLFDEAALPIGAGSFATVGIEWLKKHSD"
BASE COUNT          886 a          572 c          772 g          807 t
ORIGIN      
        1 gggtgccgaa tagtggaaaa gggcatcatg tatacgggtc tggcgagcca gtataaggaa
       61 gaactggaag aaaaactgat gaacgttatg gataccgaaa tcggtcttga tatcgtcaat
      121 ctcggattga tttatggcat cgatttagat gatgaagggg tttgcaacat ccgcctaacc
      181 ttcacgggtg ccggctgttc ttgttccgag catattctga agggggtcca tgagcagttg
      241 gatccgctgg ggttcatcac agaggtcaaa atcaaaatcg tctggagtcc ggcctgggtg
      301 ctggatcgca tcacccgcta cggccgcatt tcgcttggaa tcaatccagg cagataagca
      361 aaaggatcga aacaaagtgt atcaaaaaca ctttgttccg atcctttttt tgaatgaacg
      421 caagcatgga aaaaatgagg aatgaaagga gatatgctac aatttaagta agcatataca
      481 acgatcagaa ccaaatgaag ttgaacagat ttccaggaac aaaagtgaac actttcaagc
      541 acagcaaaaa atatgccagg caaaggagta aggatcatgc taaaacttat gagaagagtt
      601 cccggaggaa ctttattgat cccgatgttt ttaagtgcgc tgttgaacac ggcttttccg
      661 gaagggcttt atgtaggatc gattagccaa gcttttttct ccgctcaggg aaccaactat
      721 attgtaggat ttttatgttt tgtctcagct atggggttgg atgttaaaag cttggctcaa
      781 ataatgaaaa aacaaggcat gttactggtg atcaaattta tgatagcgta tgtcttggga
      841 ttcctgatga tcaagtattt gggtggggga acgattctcg gactttcgtc tttggcgatc
      901 attgtcgttc ttaccagtac aaatccagcg ttgtatatgg ctctggttaa agacttgtca
      961 gaaaaagacg atgcattggc atttggttta gcgggtgtgt tttgtgtgcc tgctgtccct
     1021 ctgttggttt attccttatc aagcggtact gatatagtct ggacgccgat tatctcagtt
     1081 gtgattcctc tgttggcggg gatgatcatc gggaatttgg atcatgaatt cagagatttt
     1141 tgcttgccgg ctatcggcat catcatgcct ttttttggat gggttttagg agacagcatc
     1201 aatctgtttc aggcggcaaa agctgggctg gcggggatta tcttaagcgt cctattttat
     1261 atcatcctct tgccgacttt atattttacg gaaacgaagc tgttgaaagc gaatggacta
     1321 tccgcgattg cgttgacctc tgtagccggc gtgtctttag cggccccaag tgttattgca
     1381 caagcctacc ctgaacttga accgattgta cccttggtcg tatctcaaat cgcgatggca
     1441 gtggtaatca caagtgttct gacaccgatg atcgcaaata aaattgcaaa gaaaaagaat
     1501 ctcgtcaagc attagaaagg ggcaggaagc atgaataaat actatgaaag agcctatgcg
     1561 ctggaagagc aacttgtaaa ggacagaagg acacttcacc aaaacccaga aataggcatg
     1621 gagttgccca atacaagagc ctacgtcagg aacagattgg aagagatcgg gttggaagtc
     1681 aaagaagtgg ggaagatggg gctgagtgcc gtcatagaag gggagaaacc tggcaaaacg
     1741 attcttcttc gtgctgacat ggatgccctt ccgatgaagg aaatgaatga gctggagtac
     1801 aaagcgacga acaatatggc gcacacgtgc ggacatgatt tgcacaccgc tatgctggtg
     1861 actgctgcac aaatattgct tgaaaataaa gcggacattc acggtagggt caagcttatg
     1921 ttccaaccag gcgaggagat atttgctgga gcgaaagata tgattgaagc cggcatttta
     1981 gaaaatccca agccggatgt ggcgtttgct atgcatacgg gattaaatca aggtgtcgga
     2041 agttttgagt attataaggg acatacaagc acctcttgtg acaattttaa gatcacaatt
     2101 acgggaaagg gtgcccacgg agcgtatcca catacttcca tagaccctat caatgcaggc
     2161 gtgatcattt atcaagagtt tggtgaactg atctcaagag aagtgagtcc gttgtctgta
     2221 gctaccttaa cattcggaat gttctcggga ggatcgaaca gcaacatcat tcctgaagtt
     2281 gtagagatgc aaggaacatt acgcacctac gatgatgaag tgcgggagta tgtaaaaggg
     2341 agaataacgg atattctggc gggaatagag aaaacgacaa gggcaaaaat agactttgaa
     2401 atatttgcga gtgtgccatc tttatacaat gatcctgact tgaccgaaga aattgcggaa
     2461 attttggaaa ccgggaatcc tgcctttaaa ggctatccgg atttgaggat catggcttct
     2521 gaagatatgg ctgttgtgtc caggcatttg ccgacaacct atatcatgct taattgtaaa
     2581 gttgaaggaa ataacttctc gcaccataat ccgggtgtgt tatttgatga agcggctctt
     2641 ccgatcggag cgggttcttt tgcgactgtg ggcatcgaat ggttaaaaaa gcacagtgac
     2701 taactaaatg tcagtaactt aatgctcagc gtggtggcca aaactgtcga ataggtgagg
     2761 tagattcgac agtttgacac cagattggac cgaaattgtc gaatgctgag aaactttcga
     2821 caatttgaca tcctgctgga tcgaaattgt cgaatatctg aggaacattc aacaatttgg
     2881 ccccgcagtc aaaaaaaagc gagaatgcat caggcatcct cgcttttttt acagttgtca
     2941 acttgcttaa gtgaatggca tcgcttggta tcaaaaatga aacaagcgat cgataggtca
     3001 tgttttttta ccggagaacg ggcgccatca aaacttt
//
LOCUS       DAOMUJ010000197         3036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9255_length_3036_cov_2.138880, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000197 DAOMUJ010000000
VERSION     DAOMUJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_3036_cov_2.138880"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>223
                     /locus_tag="PLT73_05755"
     CDS             2..>223
                     /locus_tag="PLT73_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM19919.1"
                     /translation="MLFMALGFFIFGSAQHQVVEATETQKFVIATDTTFAPFEFQDAN
                     GDFVGIDMELIQAIAEDQGFEVEIRPLGFN"
     assembly_gap    224..258
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <259..>750
                     /locus_tag="PLT73_05760"
     CDS             <259..>750
                     /locus_tag="PLT73_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM19920.1"
                     /translation="DFSEPYFESGVVMAVADDSDISSYEDLKGQNVAVKTGTEGATFA
                     ESIKDEYGFTTTVFEDSANMYQDVLSGNSVAAFEDYPVMAYAIEDQGLALKLATEKEA
                     GSSYGFAVAKDSNPELLAMFNAGLANLKENGTYQTIQEKYLGASVDTSLDVPAQNGKT
                     FIIA"
     assembly_gap    751..760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <761..2115
                     /locus_tag="PLT73_05765"
     CDS             <761..2115
                     /locus_tag="PLT73_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HRM19921.1"
                     /translation="AQTKEKYIIATDTTFAPFEFQDANGNYVGIDMDLIRAIAKDQGF
                     GIEIRPLGFNAALQALEAKQVDAVIAGMSITDERKQTFDFSEPYFESGVVMAVAADSD
                     IDSYEDLEGLTVAVKTGTEGATFAESIKDEYGFTLSTFDDSANMYQDVIAGNSVAAFE
                     DYPVMAYAIEGQGIALKLATEKEAGGSYGIAVAKDTNAELLNMINTGLANLQSNGTYE
                     EILNDYLGSDTEASNTSFFGLIQENWKTLLQGLGRTILITFASIVIALALGIFIGLFS
                     VSPNKALNWIATIYVDIMRGVPLIVLAFFVYFSVPQLMGIRMSSTVAGIITLSLNAAA
                     YLAEIFRGGIKAVDVGQMEAGRSLGLTYQTTMNKIILPQAIRIMIPSFINQFVITLKD
                     TSILSIIGLVELTQTGKIIIARTYASGNMWLIIGLMYIIVITILTKISKNLEGRLKNG
                     "
     gene            2108..2842
                     /locus_tag="PLT73_05770"
     CDS             2108..2842
                     /locus_tag="PLT73_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM19922.1"
                     /translation="MANAKINVSNLKKSFGSNEVLKGIDLEVAEGEVVCIIGPSGSGK
                     STLLRCLNKLEEVTDGHVLIDGQDITEKTTDINKVREEIGMVFQHFNLFPHLSVIDNI
                     TLAPVELKRENKETAKARALELLETVGLSEKADAFPSSLSGGQKQRVAIARALAMNPE
                     IMLFDEPTSALDPEMVGDVLEVMKKLAKQGMTMVVVTHEMGFAREVGHRVIFMDGGYI
                     VEEGTPTEVFGNPQNPRTQDFLNKVL"
BASE COUNT          844 a          703 c          731 g          713 t
ORIGIN      
        1 gatgctcttc atggctttgg gcttttttat ttttggatca gcccagcatc aagtagtgga
       61 ggcaacagaa acacaaaagt ttgtcatcgc gacagacacc acatttgcac catttgaatt
      121 ccaggatgcg aacggcgatt ttgtcggcat cgatatggaa ctcatccaag cgattgcgga
      181 agatcaagga tttgaagtgg agattcgtcc actgggattc aatnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnncg acttttcgga gccgtacttt gaaagtggcg tggtcatggc
      301 agttgccgat gacagcgaca tctcaagcta cgaagatctg aaaggacaga acgtagccgt
      361 caaaaccgga acggaaggcg caaccttcgc cgaatccatc aaggatgaat atggcttcac
      421 gaccactgtt ttcgaagatt ccgccaatat gtaccaggac gtcctgtccg gcaattccgt
      481 tgcggcattc gaggactacc cggtaatggc ctacgccatt gaggaccaag gtttggctct
      541 gaaattggct actgaaaaag aagccggcag ttcatacggt tttgcagtag ctaaggattc
      601 aaatcctgaa ttgttggcaa tgttcaacgc gggcttggct aatctgaaag agaatggcac
      661 ttatcaaacc atccaagaaa aatacctcgg tgcttccgtt gatacgagcc tggatgtccc
      721 ggcacagaac ggaaaaacgt ttatcatcgc nnnnnnnnnn atgcacaaac gaaagaaaaa
      781 tatattatcg caaccgacac aactttcgcc ccatttgaat tccaggacgc taacggtaac
      841 tatgtcggta tcgacatgga tctgatccgt gcaatcgcga aagatcaagg tttcggaatc
      901 gaaatccggc cattgggatt caatgcggct cttcaagcgt tggaagctaa acaagtggat
      961 gctgtcatcg ccggcatgag catcaccgat gaacgcaaac agacctttga tttctcggaa
     1021 ccgtacttcg aaagtggtgt ggttatggct gttgccgccg atagcgatat cgacagctat
     1081 gaagacttgg aaggcctgac tgtggccgtg aagacaggta cggaaggcgc aaccttcgcc
     1141 gaatcgatca aagatgaata tggcttcaca ttatcgacct tcgatgattc agcaaacatg
     1201 taccaagacg tcatcgccgg caattccgtt gcggcgttcg aagattatcc ggttatggcc
     1261 tatgccatcg aaggtcaagg catcgccttg aaattggcta ctgaaaaaga agcaggcggt
     1321 tcttatggga tcgcagttgc gaaagatacg aatgccgaac tgttgaacat gatcaacaca
     1381 ggcttagcga acttacagtc caacggaacc tatgaagaaa ttctcaacga ctatctcggc
     1441 tccgacacag aagcaagcaa cacttccttc ttcggactga tccaggaaaa ctggaaaaca
     1501 ttattgcaag gattgggcag aacgatcctg atcacttttg catccatcgt gatcgcattg
     1561 gcattaggta tcttcatcgg cttgttcagc gtctccccta acaaagcatt gaactggatc
     1621 gcaaccatct acgtcgacat catgcgcggt gtgccgttga tcgtcttggc attcttcgtc
     1681 tacttctcgg ttccacaatt gatggggatc cggatgagtt cgacggttgc agggatcatc
     1741 accttgagtt tgaatgcggc agcttatctg gcagagatct tccgtggcgg tatcaaagcg
     1801 gttgatgtcg ggcagatgga agccggacgc agcttaggtc tgacttatca aacgacaatg
     1861 aacaaaatca ttttgccgca agcaatccgc atcatgattc cttcattcat caatcagttc
     1921 gtcattactt tgaaagacac ttccatttta tctatcatcg gccttgtcga gttgacacag
     1981 accggtaaaa tcatcatcgc cagaacttac gcttccggta acatgtggtt gatcatcggc
     2041 ttgatgtaca tcatcgtcat cacgatcctt acgaagattt cgaaaaacct tgaagggaga
     2101 ttaaagaatg gctaacgcaa aaataaatgt ctcgaacctg aagaaaagct tcggcagcaa
     2161 tgaggttttg aaagggatcg atcttgaagt cgctgaagga gaagttgtct gcatcatcgg
     2221 cccatccggt tcaggaaaaa gcaccctgtt gcgctgtctg aacaaacttg aagaagtcac
     2281 tgacggacac gtgctgatcg acggacagga catcaccgaa aaaacgacgg acatcaataa
     2341 agtccgcgaa gaaatcggga tggttttcca acacttcaac ctttttccgc acctttcagt
     2401 catcgataat attacactgg ctccagttga actgaaacgg gaaaataagg aaaccgcaaa
     2461 agcaagagca ctcgaattgc tggaaacagt cggtctgtcc gaaaaagctg acgctttccc
     2521 tagttcctta tccggcggtc aaaagcaaag ggtcgccatc gcacgtgcgt tggcaatgaa
     2581 tccggaaatc atgcttttcg atgaaccaac atccgcgctt gatcccgaaa tggtcggcga
     2641 cgttcttgaa gtcatgaaaa aactggcaaa acaaggaatg accatggttg ttgttacgca
     2701 tgaaatgggc tttgcaagag aagtcggaca tcgcgtcatc tttatggatg gcggatatat
     2761 cgttgaagaa ggcacgccta ccgaagtatt cggtaaccct caaaacccac gtacgcagga
     2821 tttccttaac aaagtattgt aatcacgtca aacaaaggtt gccacctaag aaagtggcaa
     2881 cctttgtttt tttttgaaat gcggaaaacg agaagcggtt gtctgtggga gtctgccccc
     2941 gaacagcccg cagctccggg gagctttggc tttgccggag ctgacaggat aacggtgacg
     3001 ctacctccgg cgaagacagt ctcaccggag gagcgc
//
LOCUS       DAOMUJ010000198         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9265_length_3034_cov_2.381336, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000198 DAOMUJ010000000
VERSION     DAOMUJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9265_length_3034_cov_2.381336"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLT73_05775"
     CDS             complement(<1..945)
                     /locus_tag="PLT73_05775"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM19923.1"
                     /translation="MRSDKIKKGIDKAPHRSLLRATGQIREEGDMDKPFIAVCNSYTD
                     IVPGHVHLRELGDIAKEAIREAGGIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMLLAAVRTNVPAIFCSGGPMKAGLSATGQALTL
                     SSMFEAVGTFKEGKMTEEEFFDMEANACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDERRELIREAAFKLMDLVKKDIKPRDIITAEAIDDAFALDMAMGGSTNTVLH
                     TLALAKEAGIDYDMNRINEIAETTPYLSKIAPSSAFSMH"
     gene            complement(1265..1903)
                     /locus_tag="PLT73_05780"
     CDS             complement(1265..1903)
                     /locus_tag="PLT73_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19924.1"
                     /translation="MSEQEIYLLLTDTGSILTRTIKLFTRKKYNHISLAFDAVLEDTF
                     SFGRKKANNPFIGGFIHEDVTSDFYSRAKCAVYALTVSEEQAKVMRAYVSSFEKDKEK
                     YRYNLLGLVPALFNKEWERKNYYFCSHFVSSVLVKGGVMQSDKPVTLMRPMDVLEALP
                     CIPIYEGVLSQFNELSMAADQQELVSEMKMTLDPDTAELYFPENFSPVPLFR"
     gene            2185..>3034
                     /locus_tag="PLT73_05785"
     CDS             2185..>3034
                     /locus_tag="PLT73_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM19925.1"
                     /translation="MYHWSELSKNYPASSIRKMAKLAAQFDDTLMLTMGEPNFETPEA
                     IKKAAQDAIAANHTHYGPNVGELSFQQAVAKKYTDQTGVPFKPNEVMATFGGTEGILH
                     VMMSILNPGDEVIIADPSYPNYLGQIMLLGAKVVPVPVYEENSFKMQATDIEHVLTNK
                     TRLIILNTPNNPMGSILDQSDMEAIVALADRHQVAILSDEVYESLIYDGKKHFSLFQI
                     PGAKENHFVVNSLSKTYAMTGWRIGYVVGNHEAIDRMAEFREGVGFCVPPFIQEAAIT
                     AITGPQD"
BASE COUNT          842 a          740 c          622 g          830 t
ORIGIN      
        1 atgcatggag aaagcagatg atggtgcgat ttttgacagg tacggagttg tttccgcaat
       61 ttcgttgatg cggttcatgt catagtcgat tccggcttct ttcgccaaag ccaatgtatg
      121 cagcactgta ttcgtcgaac cacccatcgc catatccaag gcgaaggcat catcgattgc
      181 ttcagcagtg atgatgtcac gcggtttgat gtcttttttg actaagtcca tcaatttgaa
      241 agccgcttcg cggatcagtt ctcttctctc gtcactgact gccaaagcag taccgttgta
      301 tggcaatgcc aatcccaata cttccatcaa acagttcatc gagttggcgg tgaacatacc
      361 cgcacaagaa ccgcatgttg gacaagcatt ggcttccata tcgaaaaact cttcttctgt
      421 cattttgcct tctttaaaag ttccgaccgc ttcaaacatg gatgacaatg tcagagcctg
      481 tccggtagcg gaaagacctg ccttcatagg gccgcctgag cagaaaattg ctggaacatt
      541 ggtacggaca gcagccaaca gcatacccgg tgtaatttta tcgcagtttg ggatgtagaa
      601 gacaccgtcg aaccagtgtg cgttgatgac cgtttcagcg gcatcagcaa tgatttcgcg
      661 tgacggcaac gaataacgca tcccgatgtg tcccatcgcg attccgtcat cgacaccaat
      721 cgtgttgaac tcaaaaggaa tcccgccggc ttcacggatc gcttctttgg cgatatcgcc
      781 taattctctt aaatgcacgt gaccaggcac gatatctgta taggaattgc agacagctat
      841 gaacggtttg tccatgtctc cttcttccct gatttgtccg gtcgctcgca acaaggaacg
      901 atgtggcgcc ttatctattc cctttttgat tttatcacta cgcattgctg tctccccttt
      961 ttcccttata tttataaaaa agaagccctc atgtgatcta gggcttcagc ttacgtcgag
     1021 ccctgattca ctaagaatag gactcaacta attgaatagt ttgaatccta gggcataata
     1081 atgactaata aagtatttac agcatggcaa gctgttgtac ggtgcacgtt cattcttctc
     1141 atcatactgc aactccttcc attatctttc tctaaatatt aaatccaaat tagaaataag
     1201 tcaatgattt tattttcctt ttattttcct ttcatattcc tgccactttt ctccctgcat
     1261 cgtgttaccg aaagagagga actggagaga agttttccgg aaagtaaagt tccgcagtgt
     1321 ccggatcgag ggtcattttc atttcagata ccaactcttg ctggtccgca gccatggata
     1381 gctcattgaa ttgactaaga accccttcat aaatgggtat gcagggcaaa gcttccaaaa
     1441 cgtccatcgg cctcatcagt gtcacgggct tatcggactg catcacgcct cctttaacta
     1501 atacagatga cacaaaatga gaacagaaat aataattttt cctttcccac tctttattga
     1561 acagagcagg cactaggcct aaaaggttat aacgatattt ttctttgtcc ttttcgaatg
     1621 aagaaacata cgccctcatt accttcgcct gttcctctga aaccgtcaac gcatacaccg
     1681 cacattttgc acggctgtag aaatcggatg tcacatcttc gtgtatgaag cctcctataa
     1741 aaggattatt ggcttttttc ctgccgaaac tgaaggtatc ctccaacact gcatcaaacg
     1801 ccaaggatat gtgattgtat tttttcctcg taaacagttt gatggtccgg gtcaaaatag
     1861 accctgtatc cgtcaacaat aaataaatct cctgttctga catctttgta ctcctaaatt
     1921 attattacaa aatctgactt ttacccctat cagtacattg aacgataagg cggatcatac
     1981 cgttatggaa aatgtaaagt cgttctgctt tctatagcta tcaattgatt ataccaaaac
     2041 aatcagactg caagtatttt ttcaaaaaat tatcttttgc ttctttcagc actctcatgt
     2101 gcacgccctc ccctaccgac actaggatag tcagagtata tctgataaaa tagaagaaaa
     2161 atcgaataaa gggagcaaac ctatatgtac cattggtctg aactatccaa aaactatcct
     2221 gcatccagca tccgaaaaat ggcaaaatta gccgcccaat tcgatgacac gctgatgctg
     2281 acgatggggg aaccgaattt cgaaacaccg gaagccatca aaaaagccgc acaggatgcg
     2341 attgccgcaa atcacaccca ctacggaccg aatgtcggcg agctttcctt ccagcaagcc
     2401 gtagcaaaaa aatatacgga tcagaccggt gtccccttca aaccgaatga agtcatggca
     2461 actttcggcg gaacggaagg tatcctgcat gtgatgatgt caatcctgaa tccaggcgat
     2521 gaggtgatca tcgccgatcc gagctatccg aattatctcg gccagatcat gctgttgggt
     2581 gctaaagtgg tcccggtacc cgtatacgaa gaaaattcat tcaagatgca ggctacagac
     2641 atcgaacacg tcctcaccaa caaaacgcgt ttgatcattc tgaatacccc gaataatccg
     2701 atgggttcta ttctcgatca gagcgatatg gaggctatcg tggccttggc cgatcgccat
     2761 caggttgcga ttctgtcgga tgaagtctat gaaagtctta tttatgacgg aaaaaaacat
     2821 ttcagcctgt tccagatacc cggcgcaaaa gaaaatcatt tcgtggtgaa cagcttatcc
     2881 aaaacctatg cgatgacggg ttggcggatc ggctacgtcg taggcaatca tgaagccatc
     2941 gatcgcatgg ccgagttcag ggaaggtgtc ggattctgcg tacctccatt catccaagaa
     3001 gccgcaatca ctgcaatcac cggaccgcag gatg
//
LOCUS       DAOMUJ010000199         3030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9280_length_3030_cov_2.488403, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000199 DAOMUJ010000000
VERSION     DAOMUJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_3030_cov_2.488403"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(677..1261)
                     /locus_tag="PLT73_05790"
     CDS             complement(677..1261)
                     /locus_tag="PLT73_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19926.1"
                     /translation="MKREIKEEYGIWFGRAFDGMKTIAFTLNFRQRLPGKWLPDELTG
                     TPVYHKPERLTEQQALATFKRWYNSLCRKAFGAAVARRNRVKLQNLVVKEGKISRHDH
                     TATQFHLHGVIEVPKGIRADHWVAMCEAEWIGLKWADPKNHDFTKYRDAGWIEYILKG
                     RTKEDVEDSIRLEITHLNDDVMRRLKSPAPSRLT"
     gene            1404..2009
                     /locus_tag="PLT73_05795"
     CDS             1404..2009
                     /locus_tag="PLT73_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM19927.1"
                     /translation="MRNSVEEHLAKVGKKPIREFIETVTDFGQVGEVFEAAVQQCCRE
                     KAALVLAESDPLSRQLGMLARLVQQSVPLAAAGYSDVNKVLEPLFTGARADVGSKVTK
                     PKAKKKSPRGAGRGNPDLVTARATSVTVRKKNADAFASKYGHQVLEWRDRGDTLAKIA
                     ARLNGRNLPGRRGGKWHSTSVQNLISRCRLLAEQAGESQTK"
     gene            complement(2076..>3030)
                     /locus_tag="PLT73_05800"
     CDS             complement(2076..>3030)
                     /locus_tag="PLT73_05800"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19928.1"
                     /translation="AKGRLLAFEENSLTPSLYRPFTKQWLYYSRLLNERVYQMTRIFP
                     DADAHNLIIQVAGIGARAGFSVLISSSIPGFDTIEKGQCFPLYLYEEEAGASATNDFF
                     AAQKVAGGRVRRDAITDAGLVHFQEAYPGESINKEDIFYYVYGLLHSPDYRERYADNL
                     GKELPRVPRVKIAADFWAFSKAGRNLAHWHLNYETVEPYPLTIDAKGVQSDADYRVEK
                     MKFAKKGDKSTVIYNGKITLRDIPAEAWDYVVNGKAALDWVMERQSVKTDKASGIVND
                     ANDWAIETMGNPKYPLELFQRVVTVSLETQKIVAALPKLDI"
BASE COUNT          727 a          770 c          765 g          768 t
ORIGIN      
        1 gttcggagtg tgggttggga gtatcaatgg tttaccgctt taaatttaga ggtggatcac
       61 caatactgcc aatcaagcaa tcgaatgcat cgtgtgcgac tggcatggag cattgtggcg
      121 agcacacgca catggcgtaa aaaagagcag ctcaccgtgg ggtgagctgc tctttttttc
      181 aattcggtga attcattggc gcggctggcg gggatcgaaa aaacaacaaa acaccgcatg
      241 gacactctgt tttagaaatc acttatcaaa aggtgccaac aaaagtacca acaaaataaa
      301 aagtgacctt caatccttgc gtccgtccct gtcagcggat tcaaccttca gatcgtcatc
      361 cctcgaccgt ttcaggtcat gatcttcagg tcgttcggat cataccgacc cacatctttg
      421 gtggatgtct tgccctacag gctccatcaa tctcatccgc ctagaactgt cttatagcct
      481 cttcaacttg tcgttcactt cctgaattca atttctttca tccaaggtca tctataggcg
      541 gttgttgcaa ctgccctctc tatgtcgttt cgatgtccgg tttactgccc cctgatacgt
      601 cgaatacaac agcaccgggg cgaaacctga caggccgttg aacagggcct gtaactgtac
      661 ttatggcaga ggaatttcag gtgagccgag aaggcgctgg tgacttgagg cggcgcatca
      721 catcgtcgtt caggtgagtg atttccagtc ggatcgagtc ttcgacgtct tctttggtgc
      781 gacctttcag gatgtactcg atccagcctg catccctgta cttcgtaaag tcgtggttct
      841 tggggtcagc ccatttaaga ccgatccatt cggcttcgca catggcgacc caatgatcag
      901 cacgtattcc cttgggcact tcaatcacgc catgaaggtg aaattgcgtc gcagtatggt
      961 cgtgacgact gatcttgcct tccttgacca ccaagttttg aagtttcacc cgattcctac
     1021 gggctacagc cgcaccgaac gctttgcggc ataagctgtt gtaccaccgc ttaaacgttg
     1081 caagtgcctg ctgctccgtc agacgctcag gcttgtggta caccggtgtc cccgtcagtt
     1141 catccggaag ccacttcccc ggcaggcgct gcctgaagtt caatgtgaag gcgatggtct
     1201 tcatgccatc aaacgcacga ccgaaccaga ttccatactc ttccttgatt tcacgcttca
     1261 taaatttccc cgaagttggt tgtcggcggt atttacgaaa agtgatgtat agaatgtaat
     1321 taatttgaaa ttacaattta tacgacatgg aatttattgc ctactatcgg gtgtctgaaa
     1381 aacatgggat ggcgctgaat tccttgagaa attcggttga agagcatttg gcgaaagtag
     1441 gcaaaaaacc tatccgcgaa ttcatcgaaa ccgtgacgga ctttggacag gttggggaag
     1501 tgtttgaagc ggctgttcag caatgctgcc gtgaaaaggc cgcacttgtc ttggcggaat
     1561 cagacccgct atcgcgtcaa ctgggcatgc tggcgcgact ggtacagcaa agcgtaccgc
     1621 tggctgccgc tggttattcg gatgtgaaca aagtgctgga accgctgttc accggcgcac
     1681 gggctgatgt cggttccaag gtcacaaagc cgaaggcaaa aaagaagtct ccacgcggcg
     1741 caggtcgtgg taacccagac cttgtcacag ctcgggctac ttcagtgacc gttcgcaaga
     1801 aaaacgctga tgcctttgca tccaagtacg gtcatcaggt tctggagtgg cgggataggg
     1861 gagacacgtt ggcaaagatt gcggcgcggc taaatggcag gaatttaccg ggacgacgtg
     1921 gcggaaagtg gcattcaacg tctgtacaaa atctgatttc tcgttgtcga ttgctggctg
     1981 aacaggcagg cgaatcgcag acgaagtaac gatcaacaaa aacgggcgag acaagatttc
     2041 ccttgtcgtc gcccgcctgt gtcaatcagc cccggttaaa tatccaactt tggcagcgcc
     2101 gccacaatct tctgtgtttc cagactgacg gtcacgacac gctggaacaa ttccaatggg
     2161 tacttcgggt tgcccatggt ttcaatggcc cagtcgttgg cgtcgttcac gatgccgctg
     2221 gccttgtcgg ttttgaccga ttggcgctcc atgacccagt ccagcgcggc cttaccgttc
     2281 actacgtagt cccatgcctc ggcaggaata tcgcgcagcg tgatcttgcc gttgtagatg
     2341 acagtggatt tgtcgccctt cttggcgaac ttcatttttt ccacgcgata gtcagcatcg
     2401 gattgcacgc ccttagcgtc aattgtcagc ggatatggct ccactgtttc atagttcaaa
     2461 tgccaatggg ctaggttacg tcctgctttg ctgaaggccc agaaatccgc agcgattttc
     2521 acacggggga cgcgcggcaa ttccttgccc aaattgtctg cgtaacgctc acggtaatct
     2581 ggtgaatgaa gaagcccata cacatagtaa aagatatctt ccttattaat gctttccccg
     2641 ggataggctt cttggaaatg taccaagcca gcatcggtaa tagcatcgcg acgcacccgt
     2701 cctcctgcga ctttttgcgc ggcaaaaaag tcattggtag cactggcgcc tgcctcttcc
     2761 tcatatagat aaagaggaaa gcattgtcct ttttcgatag tatcgaaacc aggtatagag
     2821 ctagaaatca gcacagaaaa tcccgcgcga gcgccgattc cagcaacttg aattattagg
     2881 ttgtgtgcat ctgcatcagg aaagatacgc gtcatttggt aaaccctttc attgagcaaa
     2941 cggctgtaat aaagccattg cttggtgaag ggccgatata agctgggagt taagctattt
     3001 tcttcaaatg caagcaagcg ccccttggca
//
LOCUS       DAOMUJ010000200         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9316_length_3016_cov_2.323539, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000200 DAOMUJ010000000
VERSION     DAOMUJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9316_length_3016_cov_2.323539"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1109)
                     /gene="mgrA"
                     /locus_tag="PLT73_05805"
     CDS             complement(120..1109)
                     /gene="mgrA"
                     /locus_tag="PLT73_05805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HRM19929.1"
                     /translation="MYQPAQNRYDKMIYNRVGNSGLKLPAISLGLWHNFGEVDLFDNS
                     RKMVQRAFDLGITHFDLANNYGPPPGSAEETFGKILKKDFLPYRDELIISSKAGYDMW
                     PGPYGEWGSKKYLTASLDQSLKRMGLDYVDIFYSHRPDPETPFEETAQALDLMVRQGK
                     ALYIGISNYSAEQTAEIAAIFKDLKTPFIIHQPAYNMYNRWIEDGLQDVLTANKLGTI
                     AFSPLAQGMLTDRYLHGIPEDSRAGRSSSPFLNAERVQETIEKSRALNEIAQRRGQTL
                     AEMAVAWILRDGKVTSVLIGASRVSQIDDNVKALENLAFSNEELDEIEAVLAK"
     gene            complement(1465..1845)
                     /locus_tag="PLT73_05810"
     CDS             complement(1465..1845)
                     /locus_tag="PLT73_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM19930.1"
                     /translation="MKFEKIHHVAIIASDYEASKAFYTEVLELPIIRENYRSERDSYK
                     LDLKLGESEIELFSFPNPPERPSGPESVGLRHLCFYVEDVEATVAELNRKGVETEPIR
                     LDDYTNKKFTFFKDPDGLPLELHE"
     gene            2087..>3016
                     /locus_tag="PLT73_05815"
     CDS             2087..>3016
                     /locus_tag="PLT73_05815"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM19931.1"
                     /translation="MHLAFQEVCEQYRQACIYVSDYVFHQNFELNSIKLNEVLYHDVR
                     QRFHLKSQLTQSTFRTVTARYKTVQTQLRRSPNKYDSGKKDGDGKEVHMQVQKDLNWL
                     WHPILFSQPQADLVRNRDWSIVDNGKTFSINTLKGRVKCGFFLNGFEEYFDGTWAFGL
                     AKLVQSGKHWFLHISVKKEIVEMELTDVKHVVGIDRGLRQLVTTYDEKDKTTFFNGNA
                     VLKKRRHYKELRRSLQARNTRNSKRRIRKMESRENRWMADVNHCLSKTLVSTYGSDTV
                     FVLEDLVGVRFATEQVAKAQRYEQVSWAFFQFGQ"
BASE COUNT          798 a          706 c          717 g          795 t
ORIGIN      
        1 aaacttgtga aacatctacg gtgatgcaat tttgagttaa aaaaatttta taaattcctg
       61 tatgtaaaaa agcttcctgt ccagcaaatt cgctgggaca ggaagcctat gtcgctttct
      121 tattttgcca ataccgcttc gatttcatcc aattcttcat tactgaaggc taggttttcc
      181 aacgctttca cgttatcatc gatttggctc actctgctgg cgccgatcag tacgctcgtg
      241 actttcccgt cgcggagaat ccaagcgact gccatctcag ccaatgtttg gccgcgtctt
      301 tgcgcgattt cgttcagtgc gcgtgatttt tcgatggttt cttgcacccg ctccgcattc
      361 aggaacgggg aagaggatct gcccgcacgc gaatcttccg gaatgccatg caaatagcga
      421 tcggtcagca tgccttgcgc caacgggctg aaggcgatgg tgcccaattt gttggccgtc
      481 agaacatcct gtaggccatc ctcgatccaa cggttataca tgttgtaggc cggttggtgg
      541 atgatgaacg gcgtcttcaa gtctttgaag atggccgcaa tttcagcagt ctgctccgca
      601 gaatagtttg agatgccgat atacagcgct tttccttgac ggaccatcaa atccaatgct
      661 tgcgcagttt cttcgaaagg tgtttccgga tccggacggt gcgaataaaa gatatccaca
      721 taatcaagcc ccatccgctt taggctttga tcaaggcttg ccgtcaaata cttcttggag
      781 ccccattcgc cataagggcc gggccacata tcatagccgg ccttgctgga aatgatcagc
      841 tcgtcccgat aaggcaggaa gtcctttttc aaaatcttac cgaatgtttc ttccgcgctg
      901 cctggaggcg gaccgtagtt gttcgccaga tcgaaatggg tgattcccag atcgaaggcg
      961 cgttggacca ttttgcgcga gttatcgaac aggtccacct cgccgaagtt gtgccaaagc
     1021 ccaagggaga tggcaggcaa tttaagaccg ctgttgccta cgcggttata gatcattttg
     1081 tcgtatctgt tttgagccgg ttgatacatg tcatcacctc aataagttaa tatatccagt
     1141 atagcgtttt cattaatgca acgcaaagta tttacggaaa acggaattaa tagttttatc
     1201 cttcaatcta cctccttgcc tgtgctccgg tgaacataag cttcgccgaa gttcagaccg
     1261 taattttcca tgcgttctcc gatgagggcg gactcgcagg agtggataac aattagcaac
     1321 actgtactcc ggcgaagggg cccttaccgg agctgaagga agatggagga agaatgccac
     1381 gaatccccaa caagaaacat tagtaggaag gccgaaaaaa cggggagcgc cctcaaaaga
     1441 gacactcccc gttctagtta atctttattc atgcagctcc agcggcaagc cgtccggatc
     1501 cttgaaaaaa gtgaatttct tgttcgtgta gtcatccagc cggatcggct cagtctctac
     1561 gcctttgcgg ttcaattcag caacagttgc ctcgacatcc tccacataga agcagagatg
     1621 gcggagcccg actgattccg gaccgctggg gcgctcaggc ggattcggaa aagagaacag
     1681 ctcaatttcg ctctccccca gcttaagatc gagtttgtag gaatctcttt ccgagcgata
     1741 gttttcgcgg atgatcggca attccagcac ctcagtgtag aaggctttgg atgcctcata
     1801 atcggaggcg atgattgcga catggtgaat tttttcaaat ttcatgattt ttatagtttt
     1861 cggaaaccca cgacttcagt cgtgggagca gaaaccgatt ggcccagcgg accaaaagat
     1921 ctccttgatt tccgagctcc ttcctctttt attttaagcg aacttacgtt ctctttttaa
     1981 taatctttca attgcgattg tagtataata gggttattga agggtggtaa attccgtgga
     2041 actaagtcta acgatcaaag ttaaattgaa gctctcgacc ccaaagatgc atttggcgtt
     2101 tcaagaagtc tgcgaacagt ataggcaggc atgcatttac gtatctgatt atgtttttca
     2161 ccaaaatttc gagttaaatt cgattaaatt aaacgaagtt ttgtaccatg atgttcgcca
     2221 gcggtttcat ttgaaatccc aattgaccca atcaaccttt cggactgtta cggcccgata
     2281 caaaaccgtc caaacacaac tccgtcgcag tcccaacaaa tatgacagcg gtaaaaagga
     2341 tggagacgga aaggaagtac acatgcaagt tcaaaaggac ttgaattggc tatggcaccc
     2401 catcctgttt tctcaaccac aagcagattt agtgcgcaat cgcgattgga gcattgtcga
     2461 taatggcaaa acgttctcaa tcaatacgct gaaggggcgc gtgaagtgcg gttttttcct
     2521 caacggcttt gaagagtatt ttgatggtac ctgggcgttt ggtttggcaa aattggtcca
     2581 gtcaggcaag cattggttct tgcatatttc ggtaaaaaaa gaaatcgtcg aaatggaatt
     2641 gacagacgtc aaacacgttg tcggaatcga cagagggttg cgtcaattag tgacaaccta
     2701 tgacgaaaaa gataaaacca ctttcttcaa cggaaacgca gtcttgaaaa agcgcagaca
     2761 ctacaaagaa ctgcgccgta gtttgcaagc acgcaacacc aggaactcca aacgtcgtat
     2821 ccggaaaatg gaaagccgag agaaccgctg gatggcagat gtgaaccact gcctgtctaa
     2881 gacactcgtg tccacctatg gttccgatac ggtatttgta ttggaagatt tggttggcgt
     2941 gcgtttcgcg acggaacaag ttgcgaaagc acaacgctat gagcaagtat cgtgggcgtt
     3001 ctttcaattc gggcaa
//
LOCUS       DAOMUJ010000201         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9319_length_3015_cov_2.525000, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000201 DAOMUJ010000000
VERSION     DAOMUJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9319_length_3015_cov_2.525000"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLT73_05820"
     CDS             complement(<1..233)
                     /locus_tag="PLT73_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HRM19932.1"
                     /translation="MLIARNASGELIPAYQASKQKGKTIGEYYCPACKGRLVLKKGDV
                     MIAHFAHHQQSDCAVFSEGETLPHLMGKQMLLE"
     gene            complement(326..988)
                     /locus_tag="PLT73_05825"
     CDS             complement(326..988)
                     /locus_tag="PLT73_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HRM19933.1"
                     /translation="MMEMENINENTIRVLIENSDLEERGITFLDLLGNQKQIESFFYS
                     ILEEVDVDNQFHESDAITFQVLPNGNGLELFISKGMNNEDDFDMANGSNQLEHVVDYI
                     KKQTKSFNERDKTAAATEEEDMAPLEVVFKFQSFDDFLELAKRMFLESGVSSLYYYDN
                     QYYLAVVYFIDEMTETNIDNEILKALEFSEESDIAVEILGEYGKLVIQDNALESARHY
                     FK"
     gene            complement(1183..1581)
                     /gene="spxA"
                     /locus_tag="PLT73_05830"
     CDS             complement(1183..1581)
                     /gene="spxA"
                     /locus_tag="PLT73_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HRM19934.1"
                     /translation="MVTLYTSPSCTSCRKARAWLEENNIPYTERNIFSEPLTEIEIKS
                     ILKMTEEGTEEIISTRSKAFQELNIDLDELPLNTLFTLIEDNPGLLRRPIILDEKRLQ
                     VGYNEDEIRRFLPREVRALELQKAQRLANF"
     gene            1970..2956
                     /gene="trpS"
                     /locus_tag="PLT73_05835"
     CDS             1970..2956
                     /gene="trpS"
                     /locus_tag="PLT73_05835"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRM19935.1"
                     /translation="MKKKIFSGVQPSGIPTIGNYIGAMKQFVQRQEDYDCTYCIVNQH
                     AITVPQDPATLTERTRGLAALYLALGIDPNKSTIFIQSEVPAHAQAAWIVQCNTYLGE
                     LERMTQFKDKSSKQETVSAGLLTYPPLMVADIILYNTDFVPVGEDQKQHMELTRDFVQ
                     RFNNKYGKGNKILSMPEPMIPEDGGRIMSIQDPTSKMSKSDKNTKGYISLLDEPKVIR
                     KKIKSAVTDSSGIIEYDKENKPGISNLLTIFSAFTDKSIDELVKEFAGSGNFKEKLAD
                     AIIAELEPMQIRYYELLSSKELDDILDAGAKQANAVASETLRRMETAIGLHR"
BASE COUNT          881 a          680 c          557 g          897 t
ORIGIN      
        1 ttctccagaa gcatttgttt tcccatcagg tgaggaagtg tttcaccttc cgaaaaaacg
       61 gcgcaatccg attgttggtg atgggcaaaa tgagcgatca tgacatcccc ttttttcagc
      121 accaacctcc ccttacaggc cgggcaataa tactccccga tagttttgcc tttttgcttt
      181 gaggcttgat aagctggaat caattcccca cttgcatttc tagcgatcaa cataaacaac
      241 cgccctttct ttctgtttcc cataatatcc gcgaatggac aagaaaaaat ccgacccagt
      301 cgagggtcgg atcagatttt tttatttatt tgaagtaatg tctggctgat tccaatgcgt
      361 tgtcttggat aaccagcttg ccgtattcgc caagtatctc aacagcgata tcagattcct
      421 cagaaaattc cagcgccttc aagatttcgt tgtcgatatt cgtttccgtc atctcatcta
      481 tgaaatagac aaccgccaaa taatactgat tatcatagta gtaaagcgaa gagacgccgc
      541 tctccaaaaa catccgtttc gccagctcca aaaagtcgtc aaaactctgg aatttgaata
      601 cgacttccaa aggtgccata tcctcttctt cagtagcagc agccgttttg tcacgttcat
      661 tgaaactctt cgtctgtttt ttgatgtaat ccactacatg ctccaattga ttggagccat
      721 tcgccatatc gaagtcgtct tcgttattca tacctttact gatgaataac tctaacccat
      781 ttccattagg cagcacttgg aaagtaattg catctgattc atgaaattga ttatcgacat
      841 ctacttcctc gaggatgctg taaaaaaagc tctcgatttg cttttgattt cctaataaat
      901 ccagaaaggt gatacctctt tcctccaaat cagaattctc aatcaaaacg cgaattgtgt
      961 tttcgttgat gttttccatt tccatcatgc ttcaccccac ttttctttcc ctatttttct
     1021 tatacccatt gtaaatcaaa tcgcaaaaat gtaaagacaa aagcacaaaa tctttcaact
     1081 tctcatttac tttctcatct attcgtttct ttccgtaaaa aagacaagta caaccgtttt
     1141 tccggtgcac ttgtctgttg attctttttt gattgctttt gactagaagt ttgccaatct
     1201 ttgcgctttt tgtaattcca gcgcgcggac ttcccttggc aagaaacgac ggatttcatc
     1261 ttcgttgtat cccacttgca aacgtttttc gtccaaaatg atcggacgac gcaacagacc
     1321 gggattatcc tcgatcaaag tgaataatgt atttaaaggt aattcgtcta agtcgatgtt
     1381 aagttcttgg aatgctttgg atcgagtgga aataatctcc tcagtgcctt cttcagtcat
     1441 ttttaggatg ctcttgattt caatttctgt caaaggttcg gagaatatat tcctttcagt
     1501 ataagggatg ttattttctt ctagccatgc acgtgccttg cgacatgagg tacaacttgg
     1561 ggaagtatat aatgttacca tctctgatct ctcctttatt cgtaagtttt gtttgtgaat
     1621 cacttacagg acttattata aaccaatcta agcacaatag ctatcctttt tttaaggact
     1681 ctacaaattt cagacacatt tcagacacat ttacactttt ttaaagtcag caaaaacatt
     1741 gtgaaacctt tatttaaagg cttttttaca tcgggcacct ttcttatgaa actttataat
     1801 ttataataaa tatcgtttga taattatttt tttccgaatt cagcctaatt aataaaaacg
     1861 cttccgctcg ttccgcaatc gttcaacttt gtgcattcga ataaaatcgt gaatacaagc
     1921 cggatttgcg ttataataaa aagtgacgta cttaacctag gaggcattta tgaaaaagaa
     1981 aatattttcc ggcgttcagc ccagcggcat cccgacaatc gggaactata tcggagcgat
     2041 gaagcaattt gtgcagcgtc aagaagatta tgactgcacc tactgcatcg taaaccagca
     2101 tgcgattacc gttccacaag atccagccac ccttacagaa aggacacgtg ggttggcagc
     2161 tctttatctg gcgcttggaa tcgatccgaa caaatcgact attttcatcc aatcggaagt
     2221 ccctgcgcat gctcaagcag cttggatcgt ccaatgcaac acttacttgg gcgaattgga
     2281 gagaatgacc caattcaaag ataaatcttc caaacaagag accgtttcgg ccggattact
     2341 gacttaccct ccattgatgg tagctgatat catcctttac aatacggatt tcgtccctgt
     2401 tggcgaagac cagaagcagc acatggaatt gacaagggac tttgtgcaac gtttcaacaa
     2461 caagtatgga aaaggcaaca aaatcctgtc gatgccggaa ccgatgattc cagaagacgg
     2521 cggacggatc atgagcatcc aagaccctac cagcaaaatg agcaaatccg acaagaatac
     2581 caaaggatac atttccttac tggatgagcc aaaagtcatc cgcaaaaaaa tcaagagtgc
     2641 cgtcaccgac tcaagcggca tcatcgaata cgataaagaa aacaagcctg gcatctcgaa
     2701 tttgttgacg attttctcgg ccttcaccga taaatcgatt gatgaattgg tcaaagaatt
     2761 tgccggttcc ggtaatttca aagaaaaatt ggcggatgcc atcatcgctg aattggaacc
     2821 aatgcagatt cgttattatg agctattgag ttccaaagaa ctggatgaca ttttggatgc
     2881 aggagctaaa caagcgaatg cagttgcttc ggaaacactg agacgcatgg aaacagcaat
     2941 cggcctccac agataattag aaaagcggta caagcccgtt ctgactcgaa agaaatttag
     3001 gaaatctggc ctcgc
//
LOCUS       DAOMUJ010000202         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9328_length_3014_cov_1.747212, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000202 DAOMUJ010000000
VERSION     DAOMUJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9328_length_3014_cov_1.747212"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLT73_05840"
     CDS             complement(<1..405)
                     /locus_tag="PLT73_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRM19936.1"
                     /translation="MSLIGKEIVEFKAHAYHKGEFIEVSSEDLKGKWSIVCFYPADFT
                     FVCPTELEDLQDQYSTLQGLGVEVYSVSTDTHFTHKAWHDHSPAIGKIEYTMIGDPSH
                     AISIGFDVLDEEGLAQRGTFIIDPDGIVQAVEI"
     gene            complement(795..2618)
                     /gene="lepA"
                     /locus_tag="PLT73_05845"
     CDS             complement(795..2618)
                     /gene="lepA"
                     /locus_tag="PLT73_05845"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010765301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRM19937.1"
                     /translation="MNLEEMKQRQKKIRNFSIIAHIDHGKSTLADRILQMTDTVADRD
                     MQAQLLDSMDLERERGITIKLNAVELTYRAEDGEDYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGVEAQTLANVYLALDNNLEIVPVINKIDLPAADPERVRAEVEDVI
                     GIDASDAVLASAKIGLGIPELLEQIVTKVPAPEGDLEAPLQALVFDSVYDPYRGVVLN
                     IRIKNGVVRPGDMIRFMANGKEYEVIEVGIFSPKPIKRDYLMVGDVGYLTAGIKTIQD
                     TQVGDTITLANNPASEALAGYRKMNPMVYCGIYPVDASDFNDLREALEKLQLNDASLQ
                     FEAESSQALGFGFRTGFLGMLHMDVIQERIEREFGLDIITTAPSVIYRVNKTDGEFLE
                     IDNPSEMPDATTIESVEEPYVKASMMVPNDYVGAVMDIAQKKRGTFVTMDYLDDFRVN
                     VVYEMPLAEIIFDFFDRLKSSTKGYASLDYELIGYRPSKLVKMDILLNGEAVDALSLI
                     VHRDFAYARGKIITEKLRGLIPRQQFEVPIQATIGNKILARTTIKALRKNVLAKCYGG
                     DISRKRKLLDKQKEGKKRMKQVGSVEVPQEAFMAVLKMEED"
BASE COUNT          786 a          729 c          621 g          878 t
ORIGIN      
        1 gatttccact gcttgaacga tgccatccgg atcgatgatg aacgttccgc gttgtgctaa
       61 gccttcttca tccaacacat caaatccgat cgaaatcgca tgcgatggat ctccgatcat
      121 ggtgtattcg atttttccga tagctggtga atgatcgtgc catgctttat gcgtgaagtg
      181 tgtgtcggtt gatacggaat aaacctctac tcccaagcct tgcaaggttg aatattggtc
      241 ttgcagatct tccaattcag tcgggcaaac gaaagtgaag tccgcagggt aaaaacagac
      301 gatgctccat tttcctttta ggtcttccga tgaaacttca ataaattcac ctttgtgata
      361 agcatgcgct ttaaattcca ctatttcttt tccgattaat gacattctga ttcctccaat
      421 tagaataatt attttagaat agtacctatc aataactata tagaatccat cattaagggt
      481 caagtattaa cacctgagaa ctgtatttat ttgataattt ttgaaaaaaa ggaattccgt
      541 aactaaaatt ccttttataa ctaggatttc ctgacttaat tattatctca catttccaga
      601 tatagtcatc ggaaacttac caaaagtaga ttttacactt ctgcccctta aaataaatcc
      661 gagaacacag cgaagccatt ctcaattagc cgtatctatt ctcaattaaa aaaagaacgc
      721 atctggataa tatcatgatc cagatgcgtt ctaatattgt atgtattcta aagaatctac
      781 agactgcctt ttggttagtc ctcttccatc ttcaggacgg ccatgaaggc ttcttgaggg
      841 acctcgacgg atccgacttg cttcatgcgc ttcttacctt ctttttgttt atcgagcaat
      901 ttacgcttac gggaaatatc cccaccgtag catttagcca gtacgttttt gcgcaaagcc
      961 ttaatggtag tccgggccag gatcttgttg ccgatcgtcg cttggatcgg aacttcgaac
     1021 tgttggcgtg gaatcaatcc gcgcagtttt tcggtgatta ttttcccgcg tgcataagcg
     1081 aaatcgcgat gcacaatcag gcttaaggca tcgactgctt caccgttcaa gaggatatcc
     1141 atcttgacca atttacttgg acggtaaccg atcagttcat agtcaaggga agcataaccc
     1201 ttcgtgctgg atttcaagcg atcgaagaaa tcaaatatga tctccgccag gggcatttca
     1261 tagacgacgt tgacacggaa atcatccaaa taatccatcg tgacgaaagt gccgcgtttt
     1321 ttctgagcga tatccatgac ggcgccgaca tagtcgttag gcaccatcat gcttgctttc
     1381 acatatggct cttcaacact ttcgattgta gtcgcatcgg gcatttctga agggttgtcg
     1441 atctccaaaa attcgccatc ggtcttgttg acgcgataaa taacagatgg cgccgtcgtg
     1501 atgatgtcca aaccgaattc gcgctcgatc cgttcttgga tgacgtccat atgcagcatg
     1561 cccaaaaacc cggtacggaa accgaagccg agtgcttgcg aagattccgc ttcgaactgc
     1621 aaggatgcat cgtttaattg tagtttttcc aaagcttcac gcaaatcatt gaagtccgat
     1681 gcatcaactg ggtagatacc acagtatacc atcgggttca ttttgcgata accggccaag
     1741 gcttcactgg caggattgtt cgccaacgtg atcgtatccc cgacctgggt gtcttggatc
     1801 gttttgatgc cggcagtcaa atagcccaca tcaccgacca tcaaatagtc gcgtttgatc
     1861 ggtttcggtg agaaaatccc cacttcaatg acttcgtact ctttgccgtt agccatgaat
     1921 ctgatcatgt cgcctgggcg gactacaccg tttttgattc ggatattcag tacgaccccc
     1981 cgataaggat catagacaga gtcaaatacc aaagcctgca acggtgcttc caagtcgcct
     2041 tctggagccg gaacttttgt gacaatctgt tccaacagct caggaatgcc caagccgatt
     2101 ttcgcgctcg ccaaaaccgc atcgctggca tcgatgccga tgacatcctc tacctcagct
     2161 ctgacgcgtt cagggtctgc ggcaggcaaa tcaatcttgt tgatgacagg cacgatttcc
     2221 aggttattgt ccaaagccaa ataaacgttg gcgagggttt gtgcttcgac cccttgcgca
     2281 gcatccacta ccagaatggc cccttcgcag gccgccaaac tgcgggatac ttcatacgtg
     2341 aagtcgacgt gtcccggtgt gtcgatcaga tggaagatat aatcttctcc gtcttcggcc
     2401 ctataagtca attcgacggc gtttagtttt atggtaatgc ctcgttctct ttcgaggtcc
     2461 atggaatcca ataattgtgc ttgcatatca cgatcggcga ctgtatcagt catttggaga
     2521 attctatccg ccaatgtgga tttcccatgg tctatatggg caatgatgga aaaatttctt
     2581 atcttttttt gtcgttgctt catctcttct aagttcatta tcgttgctca ctttctctga
     2641 cgtccattcg ttcacttaaa aattatacca atctaaagcc tttatgacaa gacgccgact
     2701 cagattggca agtctttctc acatttgttc ggaatgtaaa tcgagtggac tttcaccacc
     2761 aagttatcac ccatgccggg cacactgcga aaaaaagaat ccgaaggctc tccatcggat
     2821 tctttcccat tgaaatttaa aacgcatcaa tgatattctg caatgcttgg atggcttcat
     2881 caggcaacgg cactgtcgga tgggcagcat tgaattcttc caagaaatct tctcttagct
     2941 gcatgcgtct tctcaccaat ttcgtatagt ctttatcttc gaaatcagga tcgaaaccat
     3001 ccaccggcag ataa
//
LOCUS       DAOMUJ010000203         3008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9354_length_3008_cov_2.612259, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000203 DAOMUJ010000000
VERSION     DAOMUJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_3008_cov_2.612259"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLT73_05850"
     CDS             <1..383
                     /locus_tag="PLT73_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM19938.1"
                     /translation="GCADIEKAASYGSMEIRGRDMVNGLPKTLEIHAEDIAVAIHDVV
                     ENIILSVREVLEETPPEISADVIDHGIVLTGGGALLKSIAEVISAEAEVPVFIANDPL
                     DCVSIGTGETLKNINVYKRKNLRQ"
     gene            430..1284
                     /gene="mreC"
                     /locus_tag="PLT73_05855"
     CDS             430..1284
                     /gene="mreC"
                     /locus_tag="PLT73_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRM19939.1"
                     /translation="MNQFFNNKKLIILLISVITFISLIAYSFTNNREDTSIPQQVGND
                     VTGSIARIFSKPAEVVANFVDSVDNLMNTYEENQSLKSKIDTVYELQVKVYNLEQENA
                     RMQEELELKTTLSEYEQINASVVSRNPDSWLNQIVIDKGSQDGVEVDMSVMAGNGLIG
                     RVSEVNSTSAKVLLLTTSNDTTNRVSAEIQTESGPVHGIVNGYDENTKMLSMSQIAPD
                     AVINPGDSVITSGLGGVSPSALLIGSVKEVSMDSYGLFKQVTIEPAGEVYNIRFVTVI
                     KRLSGSGE"
     gene            1284..1808
                     /gene="mreD"
                     /locus_tag="PLT73_05860"
     CDS             1284..1808
                     /gene="mreD"
                     /locus_tag="PLT73_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRM19940.1"
                     /translation="MVQNRSLHHTYFIPVVLYVLLILDGFLINAFPGQFVSEEYILVP
                     HLALFGFVLFSYYFPKQPMQLYAVLFGLLFDSYYSGILGVYAVAFAVIVYFVKKMQRF
                     LAENVFVLALLFIVAIVMVDSFVFGFYSLMDITQLDFSAFASERLGPTIVLNIVLFIV
                     IYYPLFKLVGWMYD"
     misc_binding    1888..2137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2241..>3008
                     /locus_tag="PLT73_05865"
     CDS             2241..>3008
                     /locus_tag="PLT73_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM19941.1"
                     /translation="MKKLALLLLASTSLLAACGGGAAEDSSATGSEVSEIKETLVMGL
                     DDTFAPMGFKDESGEIVGFDVELAKEVAERLDVEITFQPIDWAMKETELASGNIDMIW
                     NGYTITEERKEKVLFSEPYLSNSQIIVTMADSDIETKADLAGKVVATQQGSAAFDAII
                     ADETGIAAEFDGGTPILYPTFNDVFNDLESGRSDAIVVDEVLGRYTMKLKGEEKFAVL
                     TDNFGDEEYGVGLRKEDVALKEAIDATMNDMREDGTYD"
BASE COUNT          852 a          558 c          765 g          833 t
ORIGIN      
        1 tcggttgtgc tgatattgaa aaggcagctt cctatggctc catggaaata agaggccggg
       61 atatggtcaa cggcctgccc aagactttgg aaatccacgc cgaggatatt gcggttgcga
      121 tccatgatgt ggtcgagaac atcatccttt ccgtaagaga agtgctggaa gagacgcctc
      181 ccgaaatatc tgctgatgtc attgaccatg gtatcgtttt gacgggtggt ggcgctttgt
      241 tgaaaagcat cgctgaagtc atttcagcag aagcggaagt tcctgtattt atcgccaacg
      301 atccattgga ctgcgtctcc atcgggaccg gtgaaacctt gaagaacatt aacgtttaca
      361 agcgtaaaaa tttaaggcag taagtataat gaatgtaatt gaaagtgatg tacgaaatga
      421 ggtgtcaatg tgaatcagtt ttttaacaat aaaaaattga ttatcctatt gatcagtgtg
      481 attaccttta tcagcttgat agcatactct tttaccaaca acagagaaga tacatcgata
      541 ccgcaacaag tagggaacga tgtaacaggc tccattgcga gaatattttc taagccagcg
      601 gaagtagtgg cgaattttgt tgattcagtc gataacctga tgaatacgta tgaagaaaac
      661 caatctttaa aaagtaaaat tgataccgtt tatgagctgc aggtaaaagt ctataatctg
      721 gagcaagaaa acgcaaggat gcaggaagaa cttgaattga aaactacttt gtcagagtat
      781 gaacagatca atgcttctgt ggtatcccgc aatcccgaca gttggctgaa ccaaatcgtc
      841 atcgataaag gatcccagga cggggtggag gtcgatatgt cggtgatggc ggggaacggt
      901 ctgatcggcc gtgtatccga agtcaattcg accagtgcga aagtcctgct tttgaccaca
      961 tccaacgata caaccaaccg tgtatcagct gaaatccaaa cggaatctgg acctgtacat
     1021 ggtattgtca atggttatga tgagaatacg aagatgcttt ccatgtccca gattgcccct
     1081 gatgcagtga tcaatccggg cgatagcgtg attacatcag gattaggcgg tgtttctcca
     1141 agcgctctgt tgatcgggag cgtcaaagaa gtatcgatgg acagttacgg tttatttaag
     1201 caagtcacaa tcgagcccgc cggtgaggtt tacaacatcc gattcgtgac ggtcatcaaa
     1261 cggctgagcg gaagtggtga gtgatggtgc aaaacagatc tttgcatcac acttatttca
     1321 tccctgtggt gctctacgtg cttttgattt tggatggctt tttgatcaat gcctttccgg
     1381 gccagtttgt cagcgaagag tatattctcg tgccgcattt ggcgttgttc ggattcgtgc
     1441 ttttttccta ctatttccct aaacagccga tgcagttgta cgcagttctt ttcggactgt
     1501 tgttcgacag ttattatagc ggcattctgg gtgtttacgc agtcgctttt gccgtaatcg
     1561 tttactttgt gaagaagatg cagagatttc tggcggaaaa tgtttttgtg ctggcgttgt
     1621 tgtttattgt tgcgatcgtc atggtggatt cattcgtttt tggcttctac agtttgatgg
     1681 atatcacgca acttgacttc agtgcatttg cttcagagcg attagggccg actattgtgt
     1741 tgaatattgt attgttcatt gtgatttatt acccactgtt caaattggtg ggctggatgt
     1801 acgattgaac ctgatgagaa aataatgaga tattttttaa tgaaatgctt gcatttgacg
     1861 aaagctgtgg tattattgtc ttaaattcta aatgcaatga agagaaaaag taacttttga
     1921 ctatttccag agagtttgcg gccggtgtaa gcaaacaata gaaataagtg aaatcacatc
     1981 tctgagcaag gataccgaaa agacagcttc cgctttctca gtaggttgac tcggttgtgc
     2041 ccgttacagc actgacgttt caaacggaac gttatgaggc ggtttctgtg aagagcccgc
     2101 aaaaaaaggt ggaaccacga cttcaatcgt cctttaactt tcaacaggaa gttaaagggc
     2161 tttttttatt ggaaacggcg tgatggggga caccacaaaa ataaggcagt aaaaagtaca
     2221 catatatgga ggagaaggat atgaaaaaat tagcattatt gttgttggca tctacaagtt
     2281 tattggctgc atgcggtgga ggtgccgctg aggattcttc tgcaactgga tcggaagttt
     2341 cagaaatcaa agaaacactg gtgatgggac tggacgatac atttgcaccg atgggcttta
     2401 aagatgaaag tggcgaaatc gtcggtttcg atgtcgagtt ggcgaaagag gttgcggaac
     2461 gcttggatgt ggaaatcact ttccaaccga tcgattgggc aatgaaggaa accgaattgg
     2521 cttccggtaa catcgacatg atctggaatg gctataccat aacggaagaa cgcaaagaga
     2581 aagtcttgtt ctcagaaccg tatttgagca acagccaaat tattgtcaca atggcggata
     2641 gtgatatcga aacaaaagct gatttggccg gaaaagtcgt tgcgacacaa caaggatctg
     2701 cggcattcga tgctatcatt gccgacgaaa cgggaattgc tgctgagttt gatgggggca
     2761 cgccgatttt gtatcctacc ttcaacgacg tgttcaatga cctggaaagc ggccgcagtg
     2821 atgcaatcgt cgttgacgaa gtgttgggcc gatacactat gaaactaaaa ggcgaggaaa
     2881 agttcgctgt tcttaccgat aattttggcg atgaagaata tggtgtgggt ctccgcaaag
     2941 aagatgtcgc gttgaaagaa gcaatcgatg caacgatgaa tgatatgcgt gaagatggaa
     3001 cctacgat
//
LOCUS       DAOMUJ010000204         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9446_length_2985_cov_1.772696, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000204 DAOMUJ010000000
VERSION     DAOMUJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_2985_cov_1.772696"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1293
                     /locus_tag="PLT73_05870"
                     /pseudo
     CDS             64..1293
                     /locus_tag="PLT73_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407011.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
     assembly_gap    1294..1303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1304..1751
                     /locus_tag="PLT73_05875"
     CDS             <1304..1751
                     /locus_tag="PLT73_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRM19942.1"
                     /translation="EIKDELSESGYLKRKQSLKNKKKKVSKPHRFRSTDGTSILVGKN
                     NLQNDQLTLKTAKKTDTWLHAKNIPGSHVIIESNNPSEETILEAANIAAYYSKFQNSA
                     NVPVDYVAVKQIRKPNGAKPGFVIYEGQKTVYVTPDKELIKSLKAD"
     gene            complement(1963..2588)
                     /gene="pyrE"
                     /locus_tag="PLT73_05880"
                     /pseudo
     CDS             complement(1963..2588)
                     /gene="pyrE"
                     /locus_tag="PLT73_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(2590..>2985)
                     /gene="pyrF"
                     /locus_tag="PLT73_05885"
     CDS             complement(2590..>2985)
                     /gene="pyrF"
                     /locus_tag="PLT73_05885"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRM19943.1"
                     /translation="PAGSDVPKLIAVTQLTSTSEEAMQNEQLIKVSLQESVLHYADLT
                     CKSGLDGVVCSAWEARMIKDHTNEDFICLTPGIRPAGSAVGDQKRVATPGRAREIGST
                     FIVVGRPITQAADPVAAYHEIKNQWNGAE"
BASE COUNT          817 a          737 c          676 g          745 t
ORIGIN      
        1 tcggattggg tattgtgtta taatgttaaa gcatattcag acatgcccat aggaggtgca
       61 tccatgtctt ttgatggtat ttttacgcgt gcgatggtga gtgaactgaa ccgagagttg
      121 gaaaacggaa gaatcggaaa agttcatcaa ccctttcagc acgaaatcat tttaacagtg
      181 cgtgccaaca gaaaaaataa acgcgtgctg ctttctgcgc atccttcata cgcgcggctg
      241 caaatcattg aagattcatt atccaatccg gagtcggctc caaacttctg tatggtgctc
      301 cgaaaaaaac tggaaggtgc aatcattgag ggcattcgcc aattgggcaa tgaccgaatc
      361 gtcatcttct ctttccggaa cttcgatgaa ctgggcgatc aacagcagtt ggaactcatc
      421 gtcgaattga tggggcgtca cagcaacatc tttttgatcg acaaggagtc cagaatcatc
      481 atcgattgcc tgaagcacgt gcctttctac cagaacagct tcaggccgct gcatccggga
      541 gtagcttacc agctgccccc tcatcaggac cgttcggatt tgtttgcgct tgaaccagaa
      601 gaaatcgaaa gcaccgttgc ccaattcgat cctgaaaccc ctttgcggaa ggctttgcaa
      661 cagacattcc aaggacttgg aagcgattct gcgcaggaaa tagcgcagta tgccactgca
      721 gaaaatcact cagtgacggc cgctatccag cttttcaagg aaaggctgga atcggtgatg
      781 ccgaccttga cggacgaaag cgaaaggaaa cagcatttta cgcccttccc ttatgaaagc
      841 ctgatcggtg aaaagcagtc gttcgctacc ctcagtcagt tattggatgc ttactatgcg
      901 gaaaagcaac acgcgaccgc atccatcaag tcgcttcaga cctgcttcag atcgtcagta
      961 ccgaacggaa gaaaaatcaa ttgaagttgc agaaactcga tcaaactttg ctggaaaccg
     1021 agaaagcgga tgttttccgg ataaaaggcg agattttgac tgcttatctg catcaattca
     1081 ataaaggcca acaagaagtt gtgctgaata atttctacga cgatgagaaa ccgatcgcga
     1141 ttactctgaa tccttccctt acgccttcac agaatgcgca gaagtacttt tcgaagtacc
     1201 agaaactgac taccgctgtg aaccacgtca acgaacagat cagtcagacg catgcggaaa
     1261 acgaatacct tgaaacaatc gaaacacaga tccnnnnnnn nnnagaaatc aaggatgagt
     1321 tgagcgaatc gggttatctg aagcgcaagc aatcactgaa gaacaaaaag aaaaaagtca
     1381 gcaaacctca tcgtttccga tccactgatg gcaccagcat cctggtcggc aaaaacaatc
     1441 tacaaaacga tcaattgacc ctgaagaccg ccaaaaagac ggatacttgg ctgcatgcca
     1501 aaaatatccc tggttcgcac gtcatcatcg agagcaacaa cccgagtgag gaaacgatac
     1561 tggaagccgc caacattgca gcttactatt ccaaattcca gaactcagcc aatgttccgg
     1621 tcgattatgt ggccgtcaag cagatccgca aaccgaatgg cgcaaaaccc ggttttgtca
     1681 tctacgaagg ccagaagacg gtctacgtga ccccagacaa agaactgatt aaatccttaa
     1741 aagcggattg atgcggcaaa aaaaagaagg taccgactcg gttatagaga gtcggtacct
     1801 ttttttctcg aaaatcatta cttgatgata ccgaggctgt ccgataacca ttcagaatcg
     1861 gacaaccata acacattcac tcgcaagctt gtccgataaa caaaacaaat gggcatcctc
     1921 atttcaggaa acccatttgc ctaccataaa ttaataaata tcctaccctt tgtaccagtt
     1981 ctcggggtct ttgctccagt ctttcagcaa gtccatttca gcttcggtga tgtacgaagt
     2041 ttcgtgcgcg acttccaaaa gtgtgctgta gttggacaat gtgtcgatcg caagaccagc
     2101 atcggcgaaa ttttttgtgc ctttagccaa ttcataagtg aagatagcta cggagcccaa
     2161 tacatttgcg ccttccaact cagctgcttt ggaagcttcg atgacgctgc cccctgtgga
     2221 aatcaagtct tcgactacga ccattttttg gccttcactg attttccctt cgattttacg
     2281 tcctgttcca tgatctttcg ctttgctgcg gatgtagacc atcggcaagt ccaaaatgtc
     2341 agcgatccaa gctgcgtgcg ggatacctgc agtcgcagtt ccagcgatca cttcggatat
     2401 ttttctttga tcagatcagc cagaccttgg gcaatctgtt tacgcactgc cgggaagctc
     2461 atggtgacac gattatcgca gtaaatgggg cttctgatgc ctgatgccca cgtatatggg
     2521 ttctgtgggc ttaagctcac tgccttgatg tccaataatg attttgctac ttcttttgct
     2581 atcgtcattt tactctgctc cattccattg gttcttgatt tcgtgatagg ctgcaaccgg
     2641 atcagctgcc tgggtgatcg gacgaccgac tacaataaaa gttgagccga tttctcttgc
     2701 acggcctggt gttgcgacac gcttctggtc tccaaccgca ctgccggccg ggcggatacc
     2761 cggtgttaag cagatgaagt cttcattcgt gtggtccttg atcatgcgcg cttcccatgc
     2821 ggaacaaacg acgccgtcaa gtccggattt gcaggtgagg tcagcgtaat gcagcacgct
     2881 ctcctgaagg ctgactttga tcaactgttc attctgcatc gcttcttcgg atgtggaagt
     2941 caattgagtg actgcaatca atttcggaac atcgcttcct gccgg
//
LOCUS       DAOMUJ010000205         2980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9456_length_2980_cov_2.985983, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000205 DAOMUJ010000000
VERSION     DAOMUJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9456_length_2980_cov_2.985983"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLT73_05890"
     CDS             complement(<1..492)
                     /locus_tag="PLT73_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRM19944.1"
                     /translation="MLTLLGGIALLVLGYFTYGRYIEKNFSIDPERTTPAEALKDGYD
                     FVPMSKSKNAIIELLNIAGTGPIFGPIMGALYGPVAYIWIVVGCIFGGAVHDYMIGMI
                     SLRNDGAHLPELASKYLGKPVKHVVNIFAMLLLLLVATVFVISPANLIASITPDWMTI
                     GIIT"
     gene            complement(650..1384)
                     /locus_tag="PLT73_05895"
     CDS             complement(650..1384)
                     /locus_tag="PLT73_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRM19945.1"
                     /translation="MRVLIVDDEPLARDELAYLLGKCEGVSAITQADSIEEALGTMLE
                     DPVDLIFLDIHLTNESGLSLAAKINKLKKPPMIIFATAYDDYAVKAFELNATDYVLKP
                     FELDRIQQAVKKAYGQYRKQQIEEPETPKQKDTGLQVLPIQMDERIYILQVPEIIAIG
                     IENGETTIYTKNMEYIIHEPLSALQKKISGQSFLRVHRAFLINLKEIKEIQPWFNHTF
                     QLTMSNGLKIPVSRSYMKDFKGKVGI"
     gene            complement(1387..>2980)
                     /locus_tag="PLT73_05900"
     CDS             complement(1387..>2980)
                     /locus_tag="PLT73_05900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM19946.1"
                     /translation="VISNYTGVEISHDQIISDQVLMKLSEGASLANTRVLTIGVTGLI
                     GGPLVGTSVGIISGIIRFFQGGEEAYIYVISSILIGLISGLYGAKTMRKNAYPTIKEG
                     FMIGAITEGVQMLSILVLSRNPQAAWDLVTFIAFPMVLVNSMGTGIFLSIIGSTLRQV
                     EQTKAVQTHDVLQLANRTMPYFRSGMNEASCTEAAKIIQQLMKVSAVSITNTERILAY
                     VGAASDHHIASNEILTELSKEVLRTGEIREVHSHDEIGCNHPGCPLQAALVIPLKAQG
                     KTIGTLKLYFTDSAKLTFVERQLAEGLGNIFSSQIELGEIELQSKLLQDAEIKSLQAQ
                     VNPHFFFNAINTVSALIRVDSEQARRLLIQLSNFFRANLQGARTNLIPLMKELMQVEA
                     YRSLEQARFPDRYQVTIAVEEGLEEVLLPPFLIQILLENAFKHAFGSRKTDNRVWIDV
                     RSSPDGVFISVRDNGEGIDTKRLEKLGKEAVPSLEGTGSALDNLNKRLTSLFGESAKL
                     HFVSSPTGTTVHCTVPYQERQG"
BASE COUNT          688 a          780 c          730 g          782 t
ORIGIN      
        1 ggtgatgatg ccgattgtca tccaatcagg cgtaatgctg gcgatcagat tggccggcga
       61 aataacaaag accgtagcta ccaacagtaa cagcagcatg gcgaagatgt tgacgacatg
      121 tttgaccggt ttgcccaagt atttgctggc caattccggc aagtgcgcac cgtcgttccg
      181 caaggaaatc atcccgatca tgtagtcgtg gacggcccct ccgaagatac agcccacgac
      241 gatccaaata taggcaaccg ggccgtaaag cgctcccata atcggtccga aaataggacc
      301 cgttccggca atattcaaca attcaatgat ggcatttttt gattttgaca tcgggacaaa
      361 atcatatccg tcctttagtg cttcagcggg ggtcgttctt tccggatcga tggaaaagtt
      421 cttttcgatg tagcgcccat aagtaaagta acctagaacc agtaaggcga ttcctccaag
      481 taaagtaagc atcttttttc ctccttcaaa ataaatgtgc ggccaacacg ttttttctat
      541 gcccttactt tactggagaa aaagaaactt ttgggtcatt tgcgcccgaa acgacaaaac
      601 tgcaggctga attgcacgcc tgcagttttg aaattcatct atttttggat cagatgccta
      661 cttttccctt aaaatccttc atataagaac ggctgacggg tatcttcaag ccgttgctca
      721 tcgtcagttg gaatgtatga ttgaaccatg gttggatttc ctttatttcc ttcagattga
      781 ttagaaaggc ccggtgaacc ctcaggaacg actgtccgct gatctttttc tggagcgcac
      841 tgagcggttc gtggatgatg tactccatat ttttcgtgta gatggtcgtt tcgccgttct
      901 cgattccgat cgcaatgatt tccgggacct gcagtatata gatccgctcg tccatctgga
      961 taggcagcac ctgcaatcca gtatccttct gcttcggtgt ttccggctct tcgatctgtt
     1021 gcttgcggta ctgtccgtag gcttttttga ccgcttgctg gatccggtcc agctcaaaag
     1081 gcttcaggac ataatcggtc gcattcagct caaacgcttt gacggcatag tcgtcatacg
     1141 ccgtggcaaa aatgatcatc ggcggcttct tcagtttgtt gatcttagca gccagtgaga
     1201 ggccactctc attcgtcagg tgaatatcca agaaaatcag gtccaccggg tcctccagca
     1261 tcgtcccgag cgcttcttcg atcgaatccg cttgggtgat ggcgctcacg ccttcgcatt
     1321 tccccagcag gtaagccaat tcatcccttg ccaatggttc gtcatccaca atcaataccc
     1381 gcatactcat ccctgccttt cttgataagg taccgtgcaa tgcacggtcg ttcctgttgg
     1441 tgaagataca aaatgcaatt tggccgattc gccgaacaga ctagtcaacc gtttattcag
     1501 attatccaat gccgatcctg tgccttccag cgacggcacg gcttctttgc ccagtttctc
     1561 caagcgcttt gtatcgatgc cttcgccgtt gtcccggacg gaaatgaaca cgccatcggg
     1621 actgcttctc acgtcgatcc acacccgatt gtcggttttc cggctgccga aagcatgttt
     1681 gaatgcattt tccagcagaa tctgaatcag gaacggcggc agcagcactt cttccaagcc
     1741 ttcctcgacg gcaatagtga cttggtagcg atccgggaaa cgggcctgtt ccaatgaacg
     1801 ataggcctct acctgcatga gttccttcat cagcggaatc aagttggtgc gggcgccctg
     1861 gagattggca cggaaaaaat tgctgagttg gatcagcagc ctgcgcgcct gttcactgtc
     1921 gacccggatc aaggccgaaa cggtgttgat ggcgttgaaa aagaaatgtg gattcacttg
     1981 cgcctgcaag gacttgattt ccgcatcctg cagcagcttg ctctggagtt cgatttctcc
     2041 caattcgatc tggctggaga agatgttgcc gagtccttcc gccaactgcc tttcgacaaa
     2101 agtcagtttg gccgaatccg tgaaatacag cttcaaagtg ccgatcgttt tcccttgcgc
     2161 tttcagcggg atgacgagcg ccgcttgcaa gggacagccc ggatggttgc agccgatttc
     2221 gtcatgggaa tggacttccc tgatttcacc tgtccgcagc acttctttgg acaattcggt
     2281 caatatttca ttggaggcga tgtggtgatc gctggccgca ccgacatagg ccaaaatccg
     2341 ttcggtattc gtgatgctga cagcggatac tttcatcaat tgttggatga ttttggccgc
     2401 ttccgtgcaa gaagcttcgt tcatgccgga gcggaaatat ggcatcgttc gattcgccaa
     2461 ttggagcaca tcatgcgttt gtaccgcttt cgtctgttct acttggcgca gcgtggagcc
     2521 gatgatggaa aggaaaatac cggtccccat gctattgacg aggaccatcg gaaacgcgat
     2581 gaacgtcacc agatcccagg ccgcctgagg attccggctc aacaccagga tgctgagcat
     2641 ctggacgcct tccgttatgg cgccgatcat aaaaccttcc tttatcgtcg gataggcatt
     2701 tttccgcatc gtttttgcgc cgtacagacc ggatatgagc ccgatcaaga tggaagaaat
     2761 cacataaata taggcctctt ccccgccttg gaagaaacgg ataatgcccg agatgatgcc
     2821 gacggatgtg ccgaccaacg gcccaccgat caagccggta acgccgattg tgagcacccg
     2881 tgtgttcgcc aaggatgcgc cttccgaaag cttcatcagc acctgatcgg atatgatttg
     2941 gtcatgactg atttcaacac ctgtgtagtt cgagatgacg
//
LOCUS       DAOMUJ010000206         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9499_length_2969_cov_2.287234, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000206 DAOMUJ010000000
VERSION     DAOMUJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9499_length_2969_cov_2.287234"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1330
                     /locus_tag="PLT73_05905"
     CDS             71..1330
                     /locus_tag="PLT73_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM19947.1"
                     /translation="MITFIHTADLHLDSPFKGIKQLEPQLFDAIYQSTFASFRELVTQ
                     AISEEVDFFLISGDIYDEENQSVKAQAFLRDELGRLDRAGIPVYLSHGNHDFLGRESL
                     KLQLPGNVTVFEKEVTTEILTTKAGERVAITGFSYPSRWVEDRMIVDYPNRNHTVDYH
                     IGMLHGYLEGLNSSEGVYAPFSLGELNAKNYDYWALGHIHKRQQLQEAPPVVYCGNTQ
                     GRNPNETEAKGAYLVTLRKGMLPTLTFLPTAPIVWEKEMMSLRGCKTLNDVLARIEER
                     MEQHRAMSESSVLFSLQFTDIQGLHPDVVKKIEDEEILDGVRQRLEQPFIYLYQLKIA
                     VDTEKELFSFDQVIKGSFSKSWTEISGDDVFYQQLDNFFQHPLIRTTFPDLKKDRSFK
                     EEALAAAKKRIVQAVAFEEEDSEDTED"
     gene            1311..>2969
                     /locus_tag="PLT73_05910"
     CDS             1311..>2969
                     /locus_tag="PLT73_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM19948.1"
                     /translation="MKIRKIEIYGYGKWVNRTFDDVQDLQVFHGKNEAGKSTLSSFIN
                     SILFGFPNARKKDQNLYVPKQAQAYGGRLFLADTRFGDVIVERVKDRNKGQAVVTLAD
                     GVQQTTKNLGRFLLGMDRETFESLYTFKIDSLLSLKKTQKEDLNHYLLSIGTSGSERL
                     LQLAKEYRDEAAKRFKPTGSVPPLNKKIQAAEKLQRKLQQAKAKNATYETSLLHLSDS
                     QKQQAELLARQQELEQEQTAIAESLRLADSYQEWKRLNKEITAVDYSEVPEDARYQWQ
                     SQQERMATSIQAQTQLQEKLAQMQKQLGEFVHVEWYKEHQSDFAALQHSVAKTQADLN
                     KITYLENEVSNNKADLAKQKMAFGLNLALPTPEPLKEEERVRLQRLAQDFKQIEEQLL
                     RLDHNIGFHEDQLETLQKEEKRLQNEKLADDAYAALQSDVQQPSQRRPTPAVTKKSPL
                     GLLFVAAAVFLVLSFVLADFRVIAWALSAVLAVGGAILTRRKQQTRTKDVASPEGSPE
                     NTMEQFLKQAAVRERISAITRELEELQDKFLELLNDREELEERGK"
BASE COUNT          859 a          651 c          801 g          658 t
ORIGIN      
        1 ggctgaacga acccgttcag cttttcttat ttaacagatt tagcatagaa tgcagagaga
       61 aggacagctt gtgattacat ttatccatac agcggatctc catctggata gcccttttaa
      121 aggaatcaaa cagctggaac cgcaactttt tgatgcgatt taccagtcca cttttgcttc
      181 ttttcgggaa ttggtgacgc aagccatctc ggaagaagtc gattttttcc tgatcagcgg
      241 ggatatctac gatgaggaga atcagagtgt gaaggcgcag gcttttctga gggatgaatt
      301 gggacggctg gatcgggcgg gtatcccggt ttatttgtcg catgggaacc acgattttct
      361 cggaagggag tcgcttaaac tgcagcttcc cggaaacgta actgtcttcg aaaaagaggt
      421 caccaccgaa attttgacta cgaaagcagg tgagcgggtg gctatcaccg gcttcagtta
      481 cccgtccaga tgggttgaag atcggatgat tgtcgattat ccgaaccgga atcatacagt
      541 cgattaccat attgggatgc tgcacggtta cctcgaaggg ttgaactctt ccgaaggtgt
      601 ctatgcgccg ttttctttag gcgaattgaa cgcgaaaaac tatgattatt gggctttggg
      661 acatatccac aaaagacagc agcttcagga ggctcctcct gtcgtctatt gcggaaatac
      721 gcaagggcgt aatccgaacg agacagaagc aaaaggtgcg tatttggtga cactccggaa
      781 aggaatgtta ccgacattga cttttctccc aacggcgccg attgtttggg aaaaagaaat
      841 gatgtcgttg cgtggctgta aaactttgaa tgatgtgctt gcgcgcatcg aggaacgtat
      901 ggagcagcat cgtgctatga gtgaatcgag cgtactgttt tcgttacagt ttacagacat
      961 ccaagggcta catcccgatg tcgtcaaaaa aatcgaggac gaagaaatac tggacggtgt
     1021 ccggcaaagg ttggaacagc ctttcattta cctgtaccag ttgaaaatag cagtcgatac
     1081 ggaaaaagaa ctgttttcgt tcgatcaggt cataaaggga agtttttcga aaagttggac
     1141 ggaaatcagc ggagatgacg tattctatca acagctggac aactttttcc agcatccgct
     1201 catcaggacc actttccctg atttaaaaaa agaccgaagc ttcaaagagg aagcgttggc
     1261 ggctgccaaa aagaggatcg tgcaggccgt cgcgtttgag gaggaagaca gtgaagatac
     1321 ggaagattga aatatacggc tacggaaaat gggtgaaccg aacctttgat gatgtgcagg
     1381 atctgcaggt cttccacggc aaaaacgaag ccggcaaaag taccttgtca tccttcatca
     1441 acagtatcct cttcgggttc cccaatgccc gcaagaaaga tcagaatctg tacgttccga
     1501 agcaagcgca agcatacggt gggcgcttgt ttttggcgga cacgcggttc ggtgatgtca
     1561 tcgtggagcg ggtgaaagat cgcaataagg ggcaagcagt cgttacgctg gcagatggcg
     1621 tgcagcagac gacaaaaaat ctcgggcggt ttttattggg gatggacagg gagactttcg
     1681 agtcccttta tacattcaaa atagacagct tgctttcttt gaaaaaaact caaaaagaag
     1741 atctcaatca ttatctgctg agcatcggca ccagcgggag tgaacgcttg ctgcaattag
     1801 cgaaagaata ccgggacgaa gcggcgaaac gtttcaaacc gacaggcagc gtcccgccat
     1861 tgaacaagaa aatacaggct gctgaaaaac tgcaaaggaa gcttcagcaa gcaaaagcga
     1921 aaaatgcgac ttatgaaacg tcgctgctgc atttgtcgga tagtcaaaaa cagcaggccg
     1981 aactattagc ccgtcaacag gaactggagc aggaacaaac tgcaatcgca gagagcttac
     2041 ggttggcgga cagttatcag gagtggaaac ggctgaacaa ggaaatcacg gcagtcgatt
     2101 acagcgaagt gccggaagat gcccgctacc aatggcagag ccagcaggaa agaatggcca
     2161 cttcgatcca agcacagacg cagctgcagg aaaaattagc gcaaatgcag aaacagttgg
     2221 gcgaatttgt ccacgtggaa tggtacaaag agcaccaatc cgattttgct gctttgcagc
     2281 acagtgtcgc caaaacgcaa gcggatctga ataagatcac gtatctggaa aatgaagtca
     2341 gcaacaacaa agccgatttg gccaaacaaa aaatggcctt tgggttgaat ctggcccttc
     2401 ccacaccgga gccgcttaag gaagaagaac gcgtccgact gcagcggctt gcccaggact
     2461 tcaaacagat cgaagaacaa ctactgcgtt tggatcacaa catcggcttc catgaagacc
     2521 agctggaaac gctccagaaa gaagagaagc gtcttcaaaa cgagaagttg gctgatgatg
     2581 cttatgcggc gctgcaaagc gatgtgcaac agccgagcca aaggaggcct acgcctgcag
     2641 ttacgaaaaa aagtccgctc ggcctgttgt ttgtggctgc agctgtattt ctggtcctgt
     2701 cctttgtgct ggcggatttc agggtaattg cttgggcgtt gtctgctgtg cttgctgtcg
     2761 gtggtgctat cctgaccaga agaaaacaac agacacgaac gaaggacgtt gcctccccag
     2821 aaggctcccc cgaaaatacg atggagcaat ttctgaagca ggctgccgtc cgcgaaagga
     2881 tctcggcaat aacaagggag ctcgaagagt tgcaggataa attcctggag cttttgaacg
     2941 atcgtgagga gctggaggaa cgcggaaag
//
LOCUS       DAOMUJ010000207         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9513_length_2966_cov_2.528341, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000207 DAOMUJ010000000
VERSION     DAOMUJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9513_length_2966_cov_2.528341"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLT73_05915"
     CDS             <1..736
                     /locus_tag="PLT73_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRM19949.1"
                     /translation="PKVVIEEYLEGEEFSLFALANGAKYYYSGVAQDHKRAYDGDKGP
                     NTGGMGAYSPVPQVSEAMIQEVLDTVVKPTVEAMVAEGIPFKGVVYAGLMITAKGLRV
                     IEFNARFGDPETQVIMNQMASDLAQVIDDILNGKDPVIEMYTDRYTLGVVVAAEGYPE
                     APMKDIPLPDLDKENADCIVYAAGVKAGEQGLLSNGGRILLIAGQGETLQAAQDKAYR
                     YLSANPIAHTFYRSDIGAKAIRFTEK"
     gene            833..1825
                     /locus_tag="PLT73_05920"
     CDS             833..1825
                     /locus_tag="PLT73_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM19950.1"
                     /translation="MVKTLIHSGKVGLEGLKWEERAEQPAGSSEIKIKVKAAGLNHRD
                     LFALSQVHEKHPVVIGSDAAGVVVAVGAEVTRFKIGDEVILYPGSDWRKNSDTAPENF
                     TILGSSEQGAMAEEIVVSEENAVHKPAHLSWEEAGVLSLSALTAYRALFTKGGVSEGM
                     KVLIPGIGGGVATYLLQFAKAAGATVYVTSRSQEKLAKALQYGADQGLLHDEDWVEAM
                     GGGKVDVVIESVGAATFDKSLDAVRRGGTIVTFGSSTGDLVTFDLRKFFYGQYTLKGS
                     TMGSFEEYEAMIRFVEEHDLHPVVDAVYPAEDFKAAFLRLENAEQTGKIALQIG"
     gene            2072..>2966
                     /gene="mnmE"
                     /locus_tag="PLT73_05925"
     CDS             2072..>2966
                     /gene="mnmE"
                     /locus_tag="PLT73_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRM19951.1"
                     /translation="MIINEFDTIAAISTPPGEGAIGIVRLSGDQAIQIADALYRSGTK
                     HLADVATHTINYGHLYDPRTGDMIDEVMVSVMYGPKTFTREDVVEINCHGGVVSVNRI
                     LQTILQNGARMAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVALHQLDGKL
                     SGMIRDIRQIILNTLAEVEVNIDYPEYDDVEEVTTKLLKERTIEVRGHIQHLLDTAKQ
                     GKILREGLQTAIIGRPNVGKSSLLNRLLREEKAIVTDIAGTTRDTIEEYVNVRGVPLK
                     LIDTAGIRETDDIVEKIGVERS"
BASE COUNT          766 a          708 c          851 g          641 t
ORIGIN      
        1 accgaaagtc gtcatcgaag agtaccttga aggggaagaa ttctcgttgt tcgcgttggc
       61 gaatggtgcg aagtattact attcaggcgt cgcacaggac cacaagcggg cttacgacgg
      121 cgacaaagga ccgaatacgg gcggcatggg cgcttattcg cctgtaccgc aagtatccga
      181 agctatgatt caagaagtgc tggatacggt cgtaaaaccg actgtagagg cgatggttgc
      241 tgaaggcatc ccattcaagg gagttgtcta tgccggcttg atgatcaccg ccaaagggct
      301 gcgcgtcatc gaattcaatg cccgtttcgg cgatccggaa acacaggtca tcatgaatca
      361 gatggcatcc gatttggcgc aagtcatcga tgacatcctg aacggaaaag atccggtcat
      421 cgaaatgtac accgatcgtt acactttggg tgtcgttgtt gccgctgaag gctatccgga
      481 agcgccgatg aaagatatcc cattgcctga tctggataag gaaaatgcgg attgcatcgt
      541 ctatgcagca ggcgtgaagg ctggcgaaca aggactattg tcaaacggcg gtcgtatcct
      601 gttgatcgct ggccaaggcg aaacgctcca agccgcgcaa gacaaagcct accgttacct
      661 gagtgcaaat ccgattgcgc acacgttcta ccgttccgat atcggagcaa aagcgatccg
      721 ttttaccgaa aaatagcaag tgagagggag gcttcgggtc tccctctttg tatttctgtc
      781 cggaaaagtt atactgaacc tgacagaact atccatttga ggagtgatcg atatggtgaa
      841 aacattgatt cacagcggaa aagttggttt ggaaggtttg aaatgggaag agcgtgcgga
      901 acaaccggca ggatcttccg agataaaaat aaaggtcaag gcagccggac tgaatcatcg
      961 agaccttttc gccttgagcc aagtccatga gaagcatccc gttgtcatcg gatcggatgc
     1021 tgccggtgtc gttgtggctg tcggagcaga ggtgacgcgt ttcaaaatcg gtgatgaagt
     1081 gatactttat cccggcagcg attggcgcaa gaacagcgac acggcaccgg aaaatttcac
     1141 gatcctcggg agttcggagc agggggcgat ggcggaggaa atcgttgttt ccgaagagaa
     1201 tgctgtccat aaaccggccc atctgtcttg ggaagaagca ggcgttttgt ccctgtccgc
     1261 tttgacagct taccgcgccc tattcacaaa aggcggagta tcggaaggca tgaaagtcct
     1321 gatcccaggc atcggcggcg gagtggccac ctacctgctg caattcgcca aggccgctgg
     1381 agcgacggtg tacgtcactt cccgttccca agaaaaattg gcgaaggcgc tgcagtacgg
     1441 agccgaccaa ggcttgctgc acgatgagga ttgggtggaa gcgatgggcg gaggaaaagt
     1501 ggatgtcgtg atcgaatcgg tcggagcagc cactttcgac aaatcgctgg atgcggtccg
     1561 cagaggcggc accatcgtga ctttcggctc atcgacgggc gatctggtca ccttcgatct
     1621 gcggaaattc ttttacggac aatacacgct gaaaggttcg acaatgggca gttttgaaga
     1681 atatgaagcg atgatccgtt ttgtggagga gcatgatctc catccggtgg tggacgctgt
     1741 ttatcctgca gaggatttca aagcggcatt cctgcggttg gaaaatgccg aacagacggg
     1801 gaaaatcgcc ttgcagatcg gctgatccgt aacagcatca cgctgtcggt gaccgcagct
     1861 ttgtatttga tgatgaaata aaatagccat tgattaaatg cttgcattgc gccggcattg
     1921 cttgtataat cggctagaaa tctatttata gtaagctgaa aagaatatcc aaagtgaact
     1981 gcagtcaaag tgcttttcct cccgcttgaa cgaaggggag gagtgggcgc tttttttgta
     2041 cgctgaaaca accgagaaag gacgataatg aatgattata aatgaatttg ataccatcgc
     2101 tgccatttcg acgccgccgg gagaaggggc aatcgggatt gtgcgtttga gcggagatca
     2161 ggcaatccag atagccgatg ccttataccg atccggtacg aagcacttgg ctgatgtcgc
     2221 cacacacact atcaattacg gccaccttta tgatccgcgc acaggcgaca tgattgacga
     2281 ggtcatggtg tccgtcatgt acggaccgaa gacattcacc cgcgaagatg tcgtggaaat
     2341 caactgccac ggcggtgtcg tgagcgtcaa ccggattctg cagacgattc tccagaacgg
     2401 cgcccgcatg gcggagcccg gggaattcac aaagcgtgct ttcctgaacg gccgaatcga
     2461 tctgtcgcag gccgaagcgg tcatggactt gatccgtgcc aagaccgatc gcgcgatgaa
     2521 tgtggctcta catcagttgg acggcaaatt gtccggcatg atccgggata tccgtcagat
     2581 catcctgaac accttggctg aagtcgaagt gaacatcgac tacccggaat acgatgacgt
     2641 cgaggaagtg acgacgaagc tcttgaagga acgcaccatt gaagtgcgcg gacacatcca
     2701 gcacttgttg gataccgcca agcaagggaa gatcctgcgc gaaggtcttc agacagccat
     2761 catcggccgc ccgaacgtag gcaaatccag tctgctgaac cgcctgttgc gtgaagagaa
     2821 agccatcgtg acggatattg ccggcactac ccgcgatacg atcgaggaat acgtgaacgt
     2881 cagaggcgtt cctttgaaat tgatcgacac tgcgggcatc cgcgaaacgg atgatatcgt
     2941 ggaaaaaatc ggcgtcgaac gcagcc
//
LOCUS       DAOMUJ010000208         2949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9586_length_2949_cov_2.193504, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000208 DAOMUJ010000000
VERSION     DAOMUJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_2949_cov_2.193504"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLT73_05930"
     CDS             <1..391
                     /locus_tag="PLT73_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019252392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRM19952.1"
                     /translation="GAVEIALRSRGTPRVANRLLRRVRDFAQVYRDGLITKEIADKAL
                     SILSVDNKGLDDLDRQILTTMIRFYNGGPVGLSTIAANIGEEMETIEDMYEPYLLQLG
                     FLQRTPRGRVATPMAYDHLGISYDTEK"
     gene            408..1436
                     /gene="queA"
                     /locus_tag="PLT73_05935"
     CDS             408..1436
                     /gene="queA"
                     /locus_tag="PLT73_05935"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRM19953.1"
                     /translation="MRTSDFDFYLPEELIAQTPLLDRSSSRLLSLDSKTGAITDKHFT
                     DMVAELQEGDALVLNNTRVLPARLHGVKPDTGGHIEVLLLNNIQGDEWETLVKPGKRV
                     KVGTVISFGDGRLTAEVTQSLEHGGRILNFKYDGVFLEILESLGEMPLPPYIKETLDD
                     QERYQTVYAKEVGSAAAPTAGLHFTADLLEEVANKGVKIVYLTLHVGLGTFRPVSVDD
                     IASHEMHSEFYQLTDEAAQALNQVRSNGGKIVAVGTTSVRTLETIGTKFDGEIKADSG
                     WTKIFISPGYSFKVVQGIITNFHLPKSTLVMLVSAFAGRDNVLAAYEYAVKEKYRFFS
                     FGDAMFIK"
     gene            complement(1536..1715)
                     /locus_tag="PLT73_05940"
     CDS             complement(1536..1715)
                     /locus_tag="PLT73_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HRM19954.1"
                     /translation="MKYIAIIFWGFILGQVSVYLGSALSGGSYDFMIATMTGIFTALI
                     VVLVSALMPKTASHK"
     gene            1859..>2093
                     /locus_tag="PLT73_05945"
     CDS             1859..>2093
                     /locus_tag="PLT73_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM19955.1"
                     /translation="MSFVHLQVISAYSLLQTTTRIEDLVRSAKTKGYQAIALTDQNVL
                     YGQVDFFKLCKKYAIQPILGIQLDLPGIISKDRT"
     assembly_gap    2094..2103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2104..>2949
                     /locus_tag="PLT73_05950"
     CDS             <2104..>2949
                     /locus_tag="PLT73_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM19956.1"
                     /translation="LVLLAKDFEGYKKLMALSTVRNQDVPDKELFSLLDDDSSRIIAI
                     TPGEKGEIESFLNGDDQQNAKAASLAWKKIFGHGNFYLGVQLHEKMKPIIPRLLLLSQ
                     ETNIPTTAMHDVRYLEPSDNFSCRVLRAIEANEKVDLQSETLDGTYYLPSCGEIERKF
                     READLVESAETTQKIADEIEIELPLHQSLLPRYPLPPGTTPQAYLRRLCEEGLRQRIT
                     APDAAYEKRLAYELEVIHEMGFDDYFLIVWDVMAYARKAKIMPGAGRGSAAGSLVSYV
                     LRITHV"
BASE COUNT          790 a          662 c          760 g          727 t
ORIGIN      
        1 aggcgctgtc gaaatcgctt tgagatccag aggcaccccg cgtgttgcca accggctgct
       61 ccggcgtgtg cgcgatttcg cccaagttta tcgcgatgga ctgatcacga aggaaattgc
      121 tgataaggcc ttatccattt taagcgtgga caataaaggg cttgatgact tggataggca
      181 aatattgacg acgatgatcc gtttctacaa tggtggcccc gttggtcttt cgacgatagc
      241 agccaacatt ggtgaagaaa tggaaacgat agaggacatg tacgaaccct atctgctcca
      301 attgggtttc ctgcagcgca ccccgcgcgg cagggtagcg actccgatgg catacgacca
      361 tttggggata tcgtatgata cagaaaaata agtgaggtat tttatcaatg aggacaagtg
      421 attttgattt ttatttgccg gaagagctga ttgcgcagac accactgctg gatcggtcat
      481 catcccggct tctttccctg gactccaaga ctggagcaat cacggacaaa catttcaccg
      541 acatggtggc ggaactacaa gaaggcgatg cgctggtgtt gaacaataca cgggtgcttc
      601 cggcgcgact gcatggcgtc aaaccggata cgggcgggca tatcgaagtt ctgcttttga
      661 acaatatcca aggagacgag tgggaaacgc tcgtcaaacc agggaagcgg gtcaaagtcg
      721 gtacagtcat ctctttcggg gatggacgct tgacggccga ggtaacgcaa tcgttggaac
      781 acggcggacg catcttgaat ttcaaatatg atggcgtatt tttggagatt ttggaatcgc
      841 tgggcgaaat gccgctgccg ccatatataa aggaaacatt ggacgaccag gaacgctatc
      901 agaccgttta cgctaaagaa gtcgggtcag cggctgctcc gacagccgga cttcatttca
      961 cggctgattt gctagaggaa gtggccaata aaggtgtcaa gattgtctat ctgacgttgc
     1021 atgtgggttt ggggactttc cgtccggtat cggtcgatga tattgccagc catgaaatgc
     1081 attcggaatt ctaccagctg acggacgagg cagcgcaagc gctgaatcag gtccgctcca
     1141 acggcggtaa aatagtggcg gttggtacga cttctgtgcg aacactggaa acgatcggca
     1201 cgaaatttga tggggaaatc aaggcggaca gcggatggac caaaattttc atttcgccag
     1261 gctattcatt caaggttgtc cagggcatca tcaccaattt ccatttgccg aaatcgacct
     1321 tggtcatgct ggtcagcgct tttgccggca gagacaacgt cttagctgct tatgagtacg
     1381 cagtcaaaga aaaataccga ttcttcagct tcggtgacgc catgttcatc aaatagagaa
     1441 aaacagaacg ggttcgttca gccaaaaaaa ccttcaggaa attgatccct gaaggttttt
     1501 tcgattgtaa tgccgtcttt ataaagaatt atcggttatt tatgggaggc cgtcttcggc
     1561 atcaacgcag aaacaagaac tacgatcagc gcggtaaaga tacctgtcat ggttgcgatc
     1621 ataaagtcat agctgccgcc ggataaggcg ctgcccagat aaacagatac ttgccctaag
     1681 ataaatcccc aaaaaatgat tgcaatatat ttcatgtcgt gtacacctct tctcgaaatt
     1741 cattttatca ctttttctga agatgacaag cattacttgt ccaacgactt tttcgcttct
     1801 tggatttgga cgcgtacgct ataatgatag tattgattag aaaaaggagg ctagagacat
     1861 gtcttttgtg catttgcagg tcatcagtgc ctattctctt ttacagacga ctacccgaat
     1921 agaggatttg gtccgcagcg ccaagacaaa aggctatcag gccatcgcat tgacggatca
     1981 aaatgtcctt tatggtcaag tcgacttctt caaactttgt aaaaagtatg ctatccaacc
     2041 cattctgggc atccaactgg atttgcccgg aatcatcagt aaagatcgca cttnnnnnnn
     2101 nnngctggtc ttgttagcga aggattttga gggctacaaa aagttgatgg ctttgtcgac
     2161 ggtcaggaat caggatgttc cggacaagga attgttttct cttttggacg atgattcaag
     2221 ccgcatcatc gcgattacac ctggagaaaa gggggaaatc gaaagctttt tgaacggaga
     2281 tgatcaacaa aacgccaaag cagccagttt ggcatggaaa aaaatcttcg gtcacggcaa
     2341 tttttatttg ggtgttcagc tccacgagaa aatgaagcca atcattccgc gactgttgct
     2401 tttgtcgcag gagacaaaca ttccgacaac agctatgcat gatgtcagat atctggagcc
     2461 gagtgataat ttcagttgcc gtgtactgag ggcgatcgaa gcgaacgaaa aagtggattt
     2521 gcaatcggag actttggatg gcacgtacta cttgccgtcc tgcggagaaa tcgaaaggaa
     2581 attccgggaa gcagatttgg tcgagtcggc tgaaacaacc cagaaaattg ccgacgaaat
     2641 tgaaatcgag ctgccgctgc atcaatcact gctgccgcgc tacccgttgc ctcccggaac
     2701 gacaccccaa gcatacctca gaaggctctg cgaagaaggg ctacgtcagc ggatcactgc
     2761 cccggatgca gcgtatgaaa aacgcttggc atacgaattg gaagtcatcc atgaaatggg
     2821 cttcgatgat tactttctta tcgtttggga tgtgatggcg tatgcccgga aagcgaagat
     2881 catgccggga gctggtcggg gttctgctgc cggttcgctc gtctcatacg tcctgcgcat
     2941 cacccatgt
//
LOCUS       DAOMUJ010000209         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9608_length_2945_cov_2.386851, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000209 DAOMUJ010000000
VERSION     DAOMUJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_2945_cov_2.386851"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..749
                     /locus_tag="PLT73_05955"
     CDS             246..749
                     /locus_tag="PLT73_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRM19957.1"
                     /translation="MNFGKLLGTLIVAFIGGWAGIRFRVPAGGMIGSLVAVAIYNYFS
                     QMGYMPANLKIIGQIIIGGTIGMNFTKSSLAEMKQVFFPAVISVVILLTVGVIAGYIL
                     HKVAGIDLITALLGTSPGGLTDMTILAAAFEADTIIVSSIHLVRLFSVILLMPIFVRI
                     ISTYINK"
     gene            complement(852..1046)
                     /locus_tag="PLT73_05960"
     CDS             complement(852..1046)
                     /locus_tag="PLT73_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM19958.1"
                     /translation="MHVTKKRPSASDGRFFVAVVRFFAVIGQEMIQSACGCPILSGYR
                     TSSGPVGIWLSDSFRLSDKQ"
     gene            complement(1051..1902)
                     /locus_tag="PLT73_05965"
     CDS             complement(1051..1902)
                     /locus_tag="PLT73_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM19959.1"
                     /translation="MKIFTDSACDLAYAYLQENDVEVFPLTTLLDTGEYEDMIEIQAD
                     KVYEMIGRGGHPKTSQVSLEKFLSGFRKAAEAGESGIYISLSAKLSGTYQAAYLAHQQ
                     LKEEFPDFDLRLVDSMSASVGEGLSVVEAIEMREAGFTLDEIESRVRFTAANVVSLFT
                     VKDLNYLADGGRLSKSSAFFGGLLNIQPLLEVINGELVPVEKLRGRKKVMNRMYERLR
                     DEADMIQEQNVFLCHSDDAEAIEEMRQYIEEHFHPRRIYVNTIGSTISSHTGLGTLGL
                     FFLKKYE"
     gene            complement(2042..>2945)
                     /locus_tag="PLT73_05970"
     CDS             complement(2042..>2945)
                     /locus_tag="PLT73_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM19960.1"
                     /translation="FYQDPNHILPQEVLVPKGVDTELLQEALGTKVHTPVRGSKRSIL
                     ELAITNSELALTEKFMLIERDTHKTVGAIQELSEALGIEYLEVIESFDHSNIQGTNPV
                     SAMVVYRDGKPERKNYRKYKIKTVEGSNEFATTQEVIRRRYSRLLREGRAMPDLILMD
                     GGKVQVHAAKEVLEDELGLDIPVAGMVKDTKHKTSSLIFGEKDEIVELSPNSQAFHLV
                     QRIQEEVHRFAITFHRQIRAKNSIASQLDQIEGVGPKTRTKILKHFKTMKSIREASYE
                     DIKALGIPEKTALLIKEELGEENG"
BASE COUNT          690 a          749 c          740 g          766 t
ORIGIN      
        1 tccgaagggc taacccgtga agctagccaa cgtcttggga tgcgtgggac attttgcaat
       61 cctgtcaaac acctgcggtg atgcattctt gagttcagaa aattttatac tatcttgcaa
      121 agaacgaaga ggaacactga aagaacaggg tgttcctctt tttgcgcgga aataaacttg
      181 actattttcg tggatagtcg aaaatgggaa gagagagtca ttctgacata agggagtgta
      241 catatatgaa ttttgggaaa ctgttgggta cattgatcgt ggcattcatc gggggctggg
      301 ctgggatccg cttccgtgtg ccagctggag gaatgatcgg ttctttggtc gcagttgcaa
      361 tttataacta tttttcgcag atgggctaca tgccggccaa tctgaaaata attggacaga
      421 tcatcatcgg cgggaccatc ggcatgaact tcacgaaaag tagtctggcc gaaatgaaac
      481 aggtgttttt tcctgccgta atttccgtgg ttattttgtt gacggtcgga gtgattgcag
      541 gctatatcct gcataaagta gccggaatcg atctgatcac agccttgctg gggacctcgc
      601 cgggtggatt gacggatatg accattctgg cggcggcttt tgaggcggac acaatcatcg
      661 tttcctccat ccatctcgtt cggctgttct cggtcatcct gttgatgccg atttttgtcc
      721 ggatcatcag cacgtacatc aataaataag caaaaaaatg atcaaccgcg aagaatcgga
      781 caaccaaacg ccgactggat cattgcttgt ccgataagcg cgaagaatcg gacaactaca
      841 cgccaattgg atcattgttt gtccgataac cggaaagaat ccgacaacca aatgccgact
      901 ggaccactgc ttgtccgata accggaaaga atcggacaac cgcaagccga ttggatcatt
      961 tcttgtccga taaccgcgaa gaatcggaca accgcaacaa aaaagcgacc atcacttgca
     1021 gatggtcgct tttttgtaac atgcatactg ttattcgtat ttcttcagga agaataatcc
     1081 caacgtaccc agaccggtat gcgagctgat tgtggagccg atcgtattca catagatgcg
     1141 gcgcggatgg aaatgctctt cgatgtattg acgcatttcc tctatcgctt cagcatcgtc
     1201 gctatgacaa aggaagacat tctgttcctg aatcatatcc gcctcatcgc gcaagcgttc
     1261 gtacatccga ttcatgactt ttttgcgtcc gcgcagtttt tcgacgggaa ccaattcacc
     1321 gttgatgact tccaataaag gttggatatt cagcaatcct ccaaaaaagg cgctggactt
     1381 cgaaagccgc ccgccatcag caaggtagtt cagatccttg acggtgaaaa gggatacgac
     1441 attggcagcc gtgaaacgga cacgtgattc gatctcatcc aaagtaaaac ctgcttcgcg
     1501 catttcgatt gcttcgacga ccgacaagcc ttcaccgacg gaagcgctca tggagtcgac
     1561 caagcgcaga tcaaaatccg ggaattcctc tttcagttgc tgatgagcca gataagccgc
     1621 ttgataggtg ccggaaagtt tagccgaaag ggagatgtag atgcccgatt cgccggcttc
     1681 ggccgctttc cggaaaccgg aaaggaattt ttccaaggac acttgactcg ttttcgggtg
     1741 gccaccgcgt ccaatcattt catagacttt gtcggcttgt atttcgatca tgtcttcgta
     1801 ttcgcccgta tccagcaaag tggtcaaagg gaagacttcc acatcatttt cctgcagata
     1861 agcataagcc agatcacaag cactatcggt aaatattttc atcagtaacg cctcctccag
     1921 cgcgagtttc atcacgggat attccgtgga ctactctaca ttataacatg tttcatccgt
     1981 ttggaaaatt tcagcgctac gacttcgtga agcggactgg agccgctgca aaaacaaagg
     2041 ctcaaccatt ctcttcgccc agttcctctt tgatcagcag cgccgtcttt tccgggatac
     2101 ccaacgcttt gatgtcttcg tagctggctt cgcggatgct cttcatcgtt ttgaaatgct
     2161 tcaggatctt cgtgcgggtt ttcggtccga caccttcaat ctggtccaat tgcgaagcga
     2221 tgctgttctt ggcgcggatt tggcgatgga aagtgatcgc gaagcggtgg acctcttcct
     2281 ggatccgctg cacgagatgg aaagcctgac tgttcggact gagctccacg atttcatcct
     2341 tctcgccgaa gatgagcgag gaagtcttgt gcttcgtgtc cttgaccatg cccgctaccg
     2401 ggatgtccaa tccgagctcg tcctccagca cttccttggc ggcatgcacc tggactttac
     2461 cgccgtccat caggatcaga tccggcatgg cgcggccttc gcgcagcaaa cggctgtagc
     2521 ggcggcggat gacttcctgc gtcgtcgcga attcattgct gccttcgacc gtcttgattt
     2581 tgtatttccg gtaatttttg cgttccggct ttccgtcccg atagacgacc atcgccgaga
     2641 ccggattggt tccctggatg ttcgaatggt cgaacgactc gataacctcc agatattcga
     2701 tgccgagcgc ctcggaaagt tcctggatgg cgccgaccgt cttgtgggtg tcacgctcga
     2761 tcagcatgaa cttttccgtc agcgccagtt cgctgttcgt gatcgccagt tcgaggatgc
     2821 tgcgcttgct gccgcggaca ggcgtgtgca ctttcgttcc gagcgcttcc tgcagcagtt
     2881 ccgtgtcgac gcctttcggc acgagcactt cctgcggcag gatatgattg ggatcctgat
     2941 agaac
//
LOCUS       DAOMUJ010000210         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9617_length_2942_cov_2.588500, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000210 DAOMUJ010000000
VERSION     DAOMUJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9617_length_2942_cov_2.588500"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLT73_05975"
     CDS             complement(<1..901)
                     /locus_tag="PLT73_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRM19961.1"
                     /translation="MSDFNQNAIASFSLHGHQYEYYKLKKLENNEKIKISKLPYSIRV
                     LLESLLRQVGESGIKEEHVVTLSNWSATETNEGEIPFKPSRVILQDFTGVPAVVDLAS
                     LRKTVHDLGGDPALINPEVPVDLVIDHSVQVDNFGSPQALIANMKMEFLRNQERYQFL
                     SWAQKAFDNYRAVPPATGIVHQVNIEYLASVVTEKEIDENRSLVFPDTLVGTDSHTTM
                     ANALGVLGWGVGGIEAEASMLGEPSFFPVPEVVGVRFINSLQEAATATDLALKVTQTL
                     REMKVVGKFVEFFGPGLSSMTLAD"
     gene            complement(1351..2142)
                     /locus_tag="PLT73_05980"
     CDS             complement(1351..2142)
                     /locus_tag="PLT73_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRM19962.1"
                     /translation="MDQIDRRHSLIKSWLHEAADLLRESFNKELEIKEKTSRTDLVTN
                     MDREIEVFLYEKITAHFPDERILGEESVGHDIQDLDGIVWIIDPIDGTLNFIKQRANF
                     AIMIGIYKDGVGHLGYIYDVVRDELYFAIRDNGAYCNDRRLPRVEDLPLSEGLVAISN
                     RLVVSDADEARRIARNSSGLRVNGSAGLETAWVASGKLVAYIAPSLAPWDIAAGLVIA
                     EEVGLVYRQVTGEKINLLQNNAVIVANSFAFLEIQNEWRFIFGPH"
     gene            complement(2191..2469)
                     /locus_tag="PLT73_05985"
     CDS             complement(2191..2469)
                     /locus_tag="PLT73_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HRM19963.1"
                     /translation="MQHYDYPFEVEWSKEEIVKVISLWNAVERAYESGISKEDFMQAY
                     REFKTVLPSVGQEKKYGNQFEKESGYSLYKVLQEIKKSEKNKIFLGER"
     gene            complement(2571..>2942)
                     /locus_tag="PLT73_05990"
     CDS             complement(2571..>2942)
                     /locus_tag="PLT73_05990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054783.1"
                     /note="E3 component of pyruvate complex; catalyzes the
                     oxidation of dihydrolipoamide to lipoamide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRM19964.1"
                     /translation="IDYRAMPGVAFTDPELATIGFTAAEAKDKGMDVKASKFPLSGNG
                     RALSLNQTEGFVRLVTTKEDNVLVGAQVVGVNASEIIAGLAIEAGMNAEDIALTIHGH
                     PSLSETIMDAAELALGMPIHI"
BASE COUNT          818 a          734 c          514 g          876 t
ORIGIN      
        1 gatctgccaa agtcatggaa gataacccag gaccaaaaaa ctcgacaaat ttgccgacta
       61 ctttcatttc gcgcaacgtc tgagtcactt tcaaggccag atcagttgcg gttgctgctt
      121 cctgcagcga attgatgaaa cgtacaccca ctacttcagg aaccgggaag aaggaaggct
      181 cgccaagcat gctggcttcc gcttctattc cgccgactcc ccaacccaat actcctagag
      241 cgttcgccat cgtcgtatgg gaatccgtac ccaccaatgt atcaggaaaa accaagcttc
      301 tgttttcgtc tatttctttt tccgtgacga ctgacgccaa atactcaata ttgacttgat
      361 ggacaatacc ggtagcgggc ggcacagccc ggtaattgtc gaaggctttt tgggcccaac
      421 tcaaaaattg ataacgctct tgattacgca ggaattccat cttcatgttc gcgatcaagg
      481 cttgtggtga cccgaagtta tcaacttgca cagaatgatc aatgaccaga tcaacaggca
      541 cttccggatt gatgagcgct ggatctccgc ctaaatcatg aaccgttttt cttaatgaag
      601 ccaagtcaac gactgccggt acccctgtga aatcctgaag gataacgcgg gatggtttga
      661 aaggtatttc gccttcattc gtttccgttg cactccagtt agataaagtg acaacatgtt
      721 cttctttgat gccgctttct cccacttgac gtaacaacga ttccagaaga actcgaatcg
      781 agtaaggcaa tttagaaatt tttatttttt cattattttc taattttttt aatttatagt
      841 attcatattg atgaccatgc aaactaaaag aagctatggc attttgatta aaatccgaca
      901 ttttataacc cccgtttatc atctgtttca cattcaactc actgaatacg aagattataa
      961 ccataattta atcaaatgta aatgttttaa ctaaaaaaaa taccgcagac aagcactgac
     1021 aatcggagaa accttacata gatttgtaag atttttcatt agaaaaatga gagaaccacc
     1081 ttccgccgat accaaaatga gattcgttct cattaaaaag cattaaaaaa aactctcata
     1141 tactcatttt tttatcattt taatcatttt tctcacattt attattttta gtctcattct
     1201 ctttctgaga aaaattatat cttttttcaa catatgataa tagaatcgca taacccttgc
     1261 ctttttatgt gaggaattct aacatctttc gtcaatcaaa aaaagtgaac aaaatacact
     1321 gatctgtatc ttgtccactt caatttatac tcaatgaggc ccaaaaatga agcgccattc
     1381 attttgaatt tccaggaaag caaagctatt ggctacaatt accgcattat tctggagcag
     1441 atttattttt tctcccgtaa cttgtctata gaccaaacct acctcttcag ctatgaccaa
     1501 ccctgccgct atatcccacg gtgccaagga aggcgcgata taagccacta attttccaga
     1561 agcaacccaa gccgtttcca gtccggcaga gccattgacg cgcaacccac tactgtttct
     1621 ggcgattcta cgcgcttcgt ccgcatcgga aacaaccaat cgattactga ttgcgaccag
     1681 cccttccgat aacggtagat cctccacccg cggcagtcta cgatcattgc aataagcccc
     1741 gttatcgcgt atcgcaaaat agagctcgtc tcttacgaca tcataaatat agcctaaatg
     1801 accaacaccg tctttataga tgccgatcat gatggcaaaa ttcgctcgct gcttgatgaa
     1861 attaagtgtg ccatcaattg gatcgatgat ccagacaatt ccatccagat cctgtatatc
     1921 gtgtcctacg ctttcttctc ccaaaatacg ctcatccggg aaatgcgcag tgattttttc
     1981 atacaggaac acctcgattt cccgatccat gtttgttacc agatccgttc gcgatgtttt
     2041 ttctttaatc tcaagctcct tgttgaacga ttcccgcagc aagtctgcag cttcatgtaa
     2101 ccaagacttg atcaggctat gccttctatc gatctgatcc atccggctcc tcccttctac
     2161 cgctcctcta cccgcgactt atccagtcgt ttaacgctcc cctagaaaga ttttattctt
     2221 ttcacttttc tttatttcct gtagcacttt gtacaaagaa taacccgatt ctttctcgaa
     2281 ctgattgccg tatttctttt cctgtccgac tgaaggcagc accgttttga attcacggta
     2341 agcttgcata aagtcctctt tactgattcc actttcataa gcgcgctcga cagcattcca
     2401 caaggagatg actttaacaa tctcttcttt ggaccactcc acttcgaacg gatagtcata
     2461 atgttgcatc ttattccctc catctattct tgtttctact atattatacc tgacaaaaaa
     2521 aataaagaga atccggatcc ggattctctt tacctgttac tattttggaa ttagatgtgg
     2581 atcggcatgc ccaaagccaa ttcggcagcg tccatgattg tttcggataa ggatggatga
     2641 ccgtggatcg tcaacgcgat atcttctgcg ttcattccag cttcaatcgc caacccagca
     2701 atgatttcgc tggcattcac accgacaact tgcgcgccga caagaacgtt atcttctttt
     2761 gtcgtaacca aacgaacaaa gccttctgtc tggttcaatg acaatgcgcg tccgttacct
     2821 gacaatggga atttggatgc tttgacatcc atacctttgt cttttgcttc ggcagcagtg
     2881 aagccgatag tagctaattc aggatccgta aaggcaaccc ctggcatagc acggtaatca
     2941 at
//
LOCUS       DAOMUJ010000211         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9619_length_2942_cov_2.027018, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000211 DAOMUJ010000000
VERSION     DAOMUJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_2942_cov_2.027018"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1360)
                     /gene="gdhA"
                     /locus_tag="PLT73_05995"
     CDS             complement(14..1360)
                     /gene="gdhA"
                     /locus_tag="PLT73_05995"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRM19965.1"
                     /translation="MTAAKDYIEKVYKKVEDRGPHQKEFLQAVRELFDTIEPALEKNP
                     HYIKWNILERMVEPERIVSFRVPWMDDKGDVQVNKGFRVQFNSAIGPYKGGLRFHPTV
                     NESILKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDAEVMRFCQSFMTELQRHIG
                     PTLDVPAGDIGVGGREIGYMYGQYKRLNGAETGVLTGKPVVMNGSLARTEATGYGLVY
                     YTQEMLKANGKSFAGQKVVVSGSGNVAIYAIQKVHEFGGTVVACSDSNGYIYDEKGID
                     VELLQDIKEVRRERLTVYAAEKATAVYKDGSVWDFDLNYDIALPSATQNEIDKEQAER
                     LVKAGVYCVSEGANMPSTLEAVEVYADNNIYYGPAKAANAGGVATSALEMAQNSAHSH
                     WTFEEVDGKLQAIMANIFKTAEKTAEEYGIPGNYLAGANIAAFVQVADAMIYQGLV"
     gene            1581..2141
                     /gene="def"
                     /locus_tag="PLT73_06000"
     CDS             1581..2141
                     /gene="def"
                     /locus_tag="PLT73_06000"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM19966.1"
                     /translation="MITMDNIIREGHPTLRRSADPVTFPLSAEDRSIAAEMMEFLSNS
                     QDEKIAEQLNLRAGVGLAAPQIDVSKRIVALLIPGEYEDDPPELAKIMFNPKIISHSI
                     ESACLKGGEGCLSVDREVPGFVVRHSRITVAYQDAEGTTHKARYKGYAAIVVQHEIDH
                     LNGVMFYDHINEQAPFAVADDVIILE"
     gene            complement(2203..2940)
                     /locus_tag="PLT73_06005"
     CDS             complement(2203..2940)
                     /locus_tag="PLT73_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM19967.1"
                     /translation="MEKVIDVQHLKKNFGQHEVLKDIDFSVNKGEVVCIIGSSGSGKS
                     TLLRCINLLEKPTAGEIIYNGENVLDSKHDIFKYRSKLGMVFQQFNLFNNLDVLHNCT
                     VGPVKILKKSQQEAEKIAMGYLEQVGMGKFINAKPEQLSGGQKQRVAIARALTMEPDA
                     LLFDEPTSALDPEMVGEVLKVMKDLANSGLTMIVVTHEMEFAREVSDRVIFMDQGVIL
                     EQGAPEEIFYHPKEDRTKQFLERYLTK"
BASE COUNT          762 a          733 c          590 g          857 t
ORIGIN      
        1 gtactgcgct ttattacact aagccttgat aaatcatggc atcggcaact tgcacaaacg
       61 ccgcgatgtt ggcgcctgcc aaataatttc ccggtatgcc atattcttca gctgtttttt
      121 cagctgtctt aaatatgttt gccatgatag cctgtagctt accgtcaact tcttcgaatg
      181 tccaatggct atgagcactg ttctgcgcca tttcgagtgc cgaagtggcg actccccctg
      241 cattggccgc tttagccgga ccatagtaaa tattattgtc cgcatagact tccacagctt
      301 ccaacgtact cggcatgttc gcgccttcgc tgacacagta aacgccagct ttgactaatc
      361 tctcagcctg ttctttatct atttcatttt gtgttgcact aggcaaagca atgtcatagt
      421 taagatcgaa gtcccaaacg ctgccgtctt tataaaccgc tgtcgctttt tcggcagcat
      481 agaccgtcaa tctttcgcga cgaacttctt tgatatcctg tagcaattcg acatcgatac
      541 ctttttcatc gtagatataa ccgttcgagt cggaacaagc tacaacagtt ccgccaaatt
      601 catgaacttt ttggatagca tagatagcca cgtttccgct tccggaaaca actacttttt
      661 gaccggcaaa cgatttacca tttgctttca gcatttcctg tgtatagtac actaagccgt
      721 atccagtcgc ttctgtacgc gccaaacttc cgttcatgac gactggttta ccggtaagaa
      781 cacctgtttc agcaccgttt aagcgtttgt attggccata catatagccg atttcacgtc
      841 caccaacacc gatatcgcca gcaggaacat ccagcgtagg gccgatatgt ctttgcagct
      901 cagtcatgaa tgattgacag aaacgcatca cttcagcatc ggatttgcct ttaggatcga
      961 aatcggatcc gccttttccg ccgccgatcg gtaatccggt caggctgttt ttgaagatct
     1021 gttcaaaacc taaaaatttc aggatgctct cattgacggt aggatgaaag cgcagaccgc
     1081 ctttataggg tccgatagcc gaattgaatt ggacgcggaa tcctttgttg acttgaacat
     1141 cccctttatc atccatccaa ggaacccgga aggaaacgat gcgttcaggc tccaccattc
     1201 tttcaagaat gttccatttt atatagtgcg gatttttttc caaagcaggc tcgatcgtat
     1261 cgaacaactc tcttaccgcc tgtaagaatt ctttttgatg cgggcctcta tcttcaacct
     1321 ttttatagac tttttcaata taatctttag cagctgtcat aaaaatcatc tcttctttcg
     1381 ctatagagtt aaaaaaacct cacaccattg tgtaatcatt ttttcatttt tctattttac
     1441 acatacccat tctatattac aagagaaaag atgagtctag cttttttttt tcgaaacgtg
     1501 tactatgtaa cgtgaagtgt tttgcagttt gcaagtccaa tcatttcaat ataaaaacca
     1561 gtaaaaggag gaatgcatgc atgatcacga tggataacat catccgtgaa ggtcatccta
     1621 cattgcgcag gtcagccgac ccggtcactt ttccgttatc tgcagaagat cgaagtatcg
     1681 ctgcagaaat gatggagttc ctttcgaaca gtcaggatga aaaaattgcc gagcaactca
     1741 acttgcgcgc aggcgttggt ttggcagcac cacaaatcga cgtatccaaa cgcatcgttg
     1801 ctttgctgat accaggggaa tacgaagatg atcctccgga attggctaaa attatgttca
     1861 accctaaaat catcagccac tcgattgaaa gtgcctgcct aaaaggtgga gaaggctgcc
     1921 tttcagtgga ccgtgaagta ccaggctttg tcgtcaggca tagccgtatt acggtagcgt
     1981 accaggatgc tgaaggaacc acgcacaaag cccgctacaa aggttatgcg gccattgtag
     2041 ttcagcacga aatcgatcat cttaacggcg ttatgttcta cgatcacatc aatgaacagg
     2101 caccctttgc agttgcagac gatgtcatca tcctcgaata ggaagaaatg aataaaaaag
     2161 aagaacggac ctgcatttag tgcagaaccg ttcttctttt ttttattttg taagatatct
     2221 ctccaggaat tgcttcgtcc gatcctcttt tggatgatag aagatttctt caggggctcc
     2281 ctgctccaga atgacgcctt gatccatgaa gatgacgcga tcggaaactt ctctcgcaaa
     2341 ctccatttca tgcgtcacga cgatcatcgt caagccgctg ttggcaagat ccttcatgac
     2401 tttcaatact tcgcccacca tctccggatc caaagccgat gtcggctcat cgaacaatag
     2461 cgcatccggc tccatcgtca acgcgcgggc gatggcaacg cgttgttttt ggccgcctga
     2521 cagctgctcc ggtttggcat tgatgaattt acccattccg acctgctcca gatagcccat
     2581 ggcgattttt tcggcttcct gttgggactt tttcagaatc ttcacaggtc ctacagtaca
     2641 attatgcagc acatccaaat tgttgaacaa attgaattgc tgaaatacca tccctaattt
     2701 actgcggtac ttgaagatat catgcttcga gtccaaaacg ttctcgccgt tgtagatgat
     2761 ttctcccgct gtcggttttt caagtagatt gatgcagcgc aatagcgtcg atttaccgga
     2821 ccctgatgaa ccgatgatac aaacaacttc ccctttattc acactgaaat cgatgtcctt
     2881 cagcacttca tgctgaccga agttcttctt cagatgctgt acgtcgatta ctttttccat
     2941 ca
//
LOCUS       DAOMUJ010000212         2935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9653_length_2935_cov_2.170486, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000212 DAOMUJ010000000
VERSION     DAOMUJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_2935_cov_2.170486"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..614
                     /locus_tag="PLT73_06010"
     CDS             57..614
                     /locus_tag="PLT73_06010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM19968.1"
                     /translation="MKIGIISGSVREGRNSAAVSEWIHEFAKKRNDGGIEYEIVALAD
                     YDLPLLGAKLPEDRQAAAGAAIQAWSEKMASFDGYVFVTPEYNHAVGGALKNALDFLK
                     PEVADKAAGFVGYGSLGGARAHENMRVILGELSVATVHTTVNFSLMTDFENMSNFVPN
                     DYHASNATAMFDELIKWSGALKTIR"
     gene            817..1503
                     /locus_tag="PLT73_06015"
     CDS             817..1503
                     /locus_tag="PLT73_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRM19969.1"
                     /translation="MVLLGSLVNSAAIILGGSIGLALKKGLSDRIASAVMNALALCVL
                     YIGVSGMLKGENILITILSMVFGTLVGEWIDLDKKINQLGDEIESRVSSENKEHSVSN
                     GFVTASLLFCVGAMAIVGALQSGLTGNHDTLFAKSLIDGIAAIVMASSLGIGVLLSAG
                     LILVYEGGITLFANVLAPLLTDSVINEMTCVGSLLIVGLALNMLKLTNLKIMNYAPAV
                     FFPILFGYFM"
     gene            1606..2181
                     /locus_tag="PLT73_06020"
     CDS             1606..2181
                     /locus_tag="PLT73_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRM19970.1"
                     /translation="MCGRYALEATKSELWERYLLGEMKEDVEERAEIFPTNYTPLIMP
                     GNELVHHKWGFVEPFAKRPLINARAETILEKPTFSQPFRTARCLVPATAFFEWEKVGD
                     EKLKRKIAVADIPIFSMAGILKTYQDENGNPFTVFSIITTAANDQMRAIHDRMPVILE
                     PEDEAFYLDQKADPKLVWKLLKPTEQRLLIE"
     gene            2443..>2935
                     /locus_tag="PLT73_06025"
     CDS             2443..>2935
                     /locus_tag="PLT73_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM19971.1"
                     /translation="MIGMDWKKKVATTLALTSALLLGACAGGTEEATDSSAASSTVSE
                     DAVHIGILQILEHESLSAARTGFLEVLEEAGYVEGDNLVVDYQNAQGDQANLQSMAER
                     LAGNNDLILSISTPASQAIANAEEENAVLFTAVTDPIDAGLVASAEEPGANITGTSDQ
                     APMD"
BASE COUNT          845 a          580 c          747 g          763 t
ORIGIN      
        1 atgctttatt tggaaataaa aatacaaaaa caacagatga aaaggggaaa acaattatga
       61 aaatcggaat tatctctgga agcgtacgtg aaggaagaaa ctcagcggca gtatcggaat
      121 ggatccatga atttgccaaa aaacgcaatg atggcggcat cgaatacgaa atcgtagctt
      181 tagcagacta tgacttgcct ttgttgggcg ctaaattacc agaagaccgt caagcggcag
      241 caggtgcagc aattcaagct tggtcagaaa aaatggcttc atttgatgga tatgtatttg
      301 tgacacctga atacaaccat gcagtcggtg gcgctttgaa aaatgcattg gatttcctga
      361 aacctgaagt tgcggataaa gcagccggat ttgttggata cggaagtttg ggcggggccc
      421 gcgcacacga aaatatgcgc gtcatccttg gcgagttgag cgtagcaact gtgcacacaa
      481 cagtgaattt ctcattgatg actgatttcg aaaacatgag caattttgtg ccgaacgatt
      541 atcacgcaag caatgcaacc gcaatgttcg atgaattgat caaatggtcc ggagctttga
      601 aaactatccg ttaatgagcg ttaaatattg aaatgaatag atgattgtga tcgtcaggga
      661 gtcctggcga tcatttcttt tcgaagacag gatcctttta tattttctcc attgattttc
      721 tgaaagtgaa cacctatact gaaacgtact gagtttgcaa gcaagcattc gaatcaagtg
      781 atcagcatac atgggaaaac aatcggaggg gattaagtgg ttttgttggg aagtttggtc
      841 aacagtgcgg ccattatttt gggaggcagt atcggtctgg ctttgaagaa aggtttatcg
      901 gataggattg ccagcgcagt gatgaacgct ttggcgttat gtgtccttta cataggcgtg
      961 agtggcatgt tgaagggcga aaatattttg atcaccatcc tatcgatggt gtttgggact
     1021 ttggtcggcg aatggatcga tctggataaa aaaatcaatc agttgggcga tgaaatagaa
     1081 agccgggtat cttctgaaaa taaggaacat tctgtttcta atgggttcgt cacagcaagt
     1141 ctattgttct gtgtcggcgc gatggcgatt gtcggagctc tgcagagcgg tttgaccgga
     1201 aatcatgaca cgttatttgc gaagtcactg attgatggca tcgcggccat cgtgatggca
     1261 tccagtctag ggattggggt actgctttca gcaggtctga tcctcgttta tgaaggcgga
     1321 attacactct ttgcgaatgt cctagcgccg cttttgactg attctgtaat caacgaaatg
     1381 acctgtgtcg gctcattgct gattgttggt ctggctttga acatgctgaa attgacgaat
     1441 ctgaaaatta tgaattatgc gcctgctgtt ttttttccta tccttttcgg atattttatg
     1501 taacgacaga aagaagacga tctccagcgg acgtcttttt tgattaacga ctccgttgcc
     1561 gttataatcg gtagcaaaga aagtcagcga aaaggggaca gaactatgtg cggacgttat
     1621 gcgctggaag cgacaaagag tgaattatgg gaaaggtacc ttttggggga aatgaaagag
     1681 gatgtcgaag agcgcgcgga aatatttccg accaactaca caccactgat aatgccgggc
     1741 aacgagcttg ttcaccacaa atgggggttt gttgagcctt tcgctaagcg tcctctgatc
     1801 aatgcccgag cagagacaat actcgaaaag ccgacattca gccaaccctt tcggacagcg
     1861 cgttgtctcg tgccggcgac tgcatttttt gaatgggaga aagtcggcga tgaaaagttg
     1921 aaacgaaaaa ttgccgtagc agatatcccg attttttcga tggccggtat cctgaagacc
     1981 tatcaggatg aaaacggaaa tccttttact gttttctcca tcatcaccac ggccgccaat
     2041 gatcagatgc gtgctatcca cgatcgcatg cctgtgatac tggaacctga agatgaagcc
     2101 ttctatctag atcagaaggc tgatccgaaa ttggtttgga agttgctgaa acctacagaa
     2161 cagcgcctgc tgatcgaatg agtaatcgtt aattgtttat aaatgagaat acgctcatct
     2221 taatttaatt taatcgttga catccttatc tgattcttat aaactgaagg caaataacta
     2281 atgtgctaga aggatcaatg caaacgcaaa caaaggtgcc gcagtcccat catcaggaaa
     2341 gcccatttgg ataattctat ctgaatgggc tttttcgtac tagggataag cggttgattg
     2401 cctaagaagt tctcgcacaa actaaaaata gggggaaatg aaatgattgg aatggattgg
     2461 aaaaagaaag tagcgacaac attggcactg acttctgctt tattgcttgg tgcatgcgcc
     2521 ggcggtacag aagaagcaac ggattcttcc gcagcaagtt cgacagtttc agaagatgct
     2581 gtgcatatcg gtattctgca gattcttgag cacgagtcct tatcggctgc ccgcacaggg
     2641 ttcttggaag tgctggagga agctgggtac gtcgaaggcg acaatctggt cgtagattac
     2701 cagaatgccc agggtgacca agccaacctg cagagtatgg ctgagcgctt agcaggcaat
     2761 aatgacctca ttttatcgat atcgaccccg gcttcacaag cgatagcaaa cgcagaagaa
     2821 gaaaatgcag ttctgttcac tgccgtaacc gatccaatcg atgccggact ggtggcaagc
     2881 gcggaagaac cgggagcgaa catcaccgga acaagcgacc aagcaccaat ggaca
//
LOCUS       DAOMUJ010000213         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9669_length_2931_cov_1.846314, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000213 DAOMUJ010000000
VERSION     DAOMUJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9669_length_2931_cov_1.846314"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLT73_06030"
     CDS             <1..393
                     /locus_tag="PLT73_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM19972.1"
                     /translation="EVGKVIEEHFHRIARGLEEMYDVKVEIDYNSDYPPLYNHPKETA
                     KAVAVLEQNGVGSYLNQVIATSANTGAEDFGQYLLKIPGAFLNVGARPNQAEVFNNHH
                     PKFDINEKALLVAAKAVADITLAALSAK"
     gene            412..969
                     /gene="folE"
                     /locus_tag="PLT73_06035"
     CDS             412..969
                     /gene="folE"
                     /locus_tag="PLT73_06035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRM19973.1"
                     /translation="MDLEKIEVAVQMILEAIGEDPKRSGLQDTPKRVARMYAEIFSGL
                     EKDPADHAKVIFQEDTDEIVLVKDIPFYSTCEHHLVPFFGVGHIAYMPKDGQVIGLSK
                     LARILDEVSKKPQIQERITTTVADILMEHLEPQGVMVVLEAEHMCMTMRGVKKPGSKT
                     VTSAVRGGFKQSFKSRSEVLELIKL"
     gene            1055..1834
                     /gene="folP"
                     /locus_tag="PLT73_06040"
     CDS             1055..1834
                     /gene="folP"
                     /locus_tag="PLT73_06040"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRM19974.1"
                     /translation="MGILNVTPDSFSDGGEWNTVEKAVAHALEMVADGADIIDIGGES
                     TRPGHTQISDEEEIARIVPVIEALKKAVDVPLSIDTYKSAVARAACEAGVDIINDIWG
                     CRYDPQIAAVAASFDVPIILMHNREKPDYAFLIEDMLTDLAESVRIAVSAGVKRENII
                     LDPGCGFGKTYEDNLNVVHHLKRFADLGYPLLLGTSRKRFIGTALGDIPFKERDLGTA
                     ATTALGIVNGAQIFRVHNVKANAEMARMMDIMLKKGESPIG"
     gene            1827..2195
                     /gene="folB"
                     /locus_tag="PLT73_06045"
     CDS             1827..2195
                     /gene="folB"
                     /locus_tag="PLT73_06045"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRM19975.1"
                     /translation="MDKIYLNNLQFYAFHGLNAEEKVLGQRFNVDVVLHTDTKKAGYS
                     DKMEDSIHYGHAYKAVKAVVEGENFNLIEALAEHIAIALFARFDGLQACQVKVIKPDP
                     PIVGHYDSVAVEIYREREGS"
     gene            2200..2700
                     /gene="folK"
                     /locus_tag="PLT73_06050"
     CDS             2200..2700
                     /gene="folK"
                     /locus_tag="PLT73_06050"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRM19976.1"
                     /translation="MPIVYIALGTNLEPRAAHLDKALELFHSLSGVEVKRVSSIYESK
                     PVGYLDQPDFLNLVFEAETELLPLDLLDSCQTIEQELGRVRTIRFGPRTLDVDIVLYG
                     VESIDEERLTVPHPRMQERSFVLLPLQELNPEYVVPEWNKTVNELVAELPAKDLEEIW
                     KYAPES"
     misc_feature    2786..2919
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
BASE COUNT          796 a          633 c          792 g          710 t
ORIGIN      
        1 gaagtcggaa aggtcatcga ggagcatttc caccggattg cccgcggtct tgaggaaatg
       61 tatgacgtga aggtagaaat cgattacaac tccgattacc cgcctttata caatcatcca
      121 aaagagactg ccaaagcagt agctgttctg gaacaaaacg gtgtcggttc ttatttgaac
      181 caagtcatcg caacttctgc caacactgga gcggaggatt tcggacagta tctgttgaaa
      241 atccccggcg ctttcctgaa tgtcggtgcc cgtccgaacc aggcagaagt gttcaataat
      301 catcacccaa aattcgacat caatgaaaaa gctctattgg tcgcagctaa agcagtcgct
      361 gacatcaccc ttgcggcgtt atccgcaaaa tgagcgttta ggaggataaa tatggattta
      421 gagaagatag aagtcgctgt ccaaatgatt ttggaggcga tcggggaaga cccgaagcgc
      481 tcagggctgc aggatacacc gaagcgcgtg gcgcgcatgt atgcagaaat tttttccggt
      541 ctcgaaaaag atccagccga tcacgccaaa gtgatctttc aagaagatac agacgaaatc
      601 gtgttagtga aggatattcc tttctattca acttgcgaac atcatctggt tccgtttttt
      661 ggtgtggggc atatcgctta tatgccgaaa gatggacagg tgatcggttt gagcaaactt
      721 gcccgcatcc tggatgaagt cagcaaaaaa ccgcagatac aggagcgcat cacgacgacg
      781 gttgcggata ttctgatgga gcatctggaa ccgcagggag tcatggtcgt tttggaagcc
      841 gagcatatgt gcatgaccat gcgcggcgtg aaaaaaccgg gcagcaagac tgtgacatcc
      901 gcagtccgtg gtggattcaa acaatccttc aaatccagaa gtgaagtact ggaattgatc
      961 aaactttaaa cgaagtacac gctgatgagg agaatggacc ttgaaattaa cgacaaagac
     1021 gaaagtatat gaactgtcac agcgcagtta cattatggga atcctaaacg tgacgccgga
     1081 ttcattttcc gatggcggag agtggaacac ggtcgaaaaa gctgtcgccc acgcgctgga
     1141 gatggttgct gatggcgccg atatcatcga catcggcggg gaatccaccc gtcctggtca
     1201 cacgcagata tctgatgaag aggagattgc gcggattgtg cccgtcattg aagcgctaaa
     1261 gaaagcggtc gatgtgccgc tttcaatcga cacttacaag tcggcggtcg ctcgagcggc
     1321 ttgcgaagcc ggcgttgata tcatcaatga catttggggc tgtaggtacg atcctcagat
     1381 tgcagctgtg gcggcgtcat ttgatgtgcc gatcattttg atgcacaatc gggaaaagcc
     1441 ggattatgcc tttttgatcg aagatatgtt gactgatttg gcggagagtg tgcggatcgc
     1501 agtgtccgca ggcgtcaaaa gggaaaacat cattctcgat cccggctgcg gattcggaaa
     1561 aacctatgaa gacaatctga atgtggtcca tcatctgaag cggtttgcgg atctcggcta
     1621 cccgttgctg ctgggaacgt cccgcaaacg gttcatcgga acggcattgg gggatatccc
     1681 cttcaaggag cgcgatctgg ggacagccgc cacgactgca ttaggcatcg taaacggtgc
     1741 gcaaatattc cgcgtgcata atgtaaaggc caatgcggaa atggcaagga tgatggatat
     1801 catgttaaaa aaaggagaga gcccgattgg ataaaatcta tttgaataat ctacagttct
     1861 atgctttcca tggattgaat gcggaagaaa aagtgttggg acaacgtttt aacgtagatg
     1921 ttgtgctgca tactgataca aaaaaagccg gttacagcga caagatggag gattccatcc
     1981 attacggaca tgcctacaag gcggtcaaag cagtagtgga gggcgaaaac tttaacctga
     2041 tcgaagcttt ggccgagcat attgccatcg ctttgtttgc gcgttttgat gggttgcaag
     2101 cctgccaagt gaaagtgatc aagccggatc caccgatcgt cggccattac gattccgttg
     2161 cggtggagat ctaccgcgaa agggaaggga gctaagcgca tgcctatcgt gtacattgca
     2221 ttaggcacga atctggagcc gcgtgcagcc catcttgata aagccttgga gcttttccac
     2281 tccttgtcgg gtgtcgaagt gaagcgcgta tcttctattt atgaatcgaa accggtaggc
     2341 tatctcgatc agcctgattt tctgaaccta gtgtttgaag cagaaacgga attgttacct
     2401 ttggatttgc tggacagctg ccaaactatc gaacaggagc tcggtagagt acggacgatt
     2461 cgattcggac cgcgtaccct ggatgtcgac attgttttgt acggagtgga gagtatcgat
     2521 gaagaacggc ttactgtgcc gcatccgaga atgcaggagc gctccttcgt actgttgccg
     2581 ctgcaggaac tgaatccgga gtatgtcgtt ccggaatgga ataagacagt caatgaatta
     2641 gtggcggaat tgccagcgaa ggatctcgaa gaaatctgga aatacgcacc ggaatcctga
     2701 atgtctgaac gtatattgaa aatgaagatt tttgttgaaa tgttcttgac gttttcattt
     2761 taacaaggta tactgtaaaa gttgagaaat taagcagagg cacccgcttc tcgcctaagt
     2821 ggacaattgc gtccctgggc cagatatgta caagtaactt gcagcaatgc gagtattgtc
     2881 ggcggggtta gtatgacccg ccgacttttt tctgcgttct ttctctcaaa t
//
LOCUS       DAOMUJ010000214         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9721_length_2918_cov_1.804052, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000214 DAOMUJ010000000
VERSION     DAOMUJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9721_length_2918_cov_1.804052"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="leuB"
                     /locus_tag="PLT73_06055"
     CDS             <1..832
                     /gene="leuB"
                     /locus_tag="PLT73_06055"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRM19977.1"
                     /translation="AIGGPKWEKAEKTPEDGLLQLRKTLGLFSNIRPIAVSDSIAHLS
                     PLKTERVKGTDFIVVRELTGGLYFGQPKHWNDEEATDTLFYKKSEIDLIVRQAFDIAM
                     TRGKKLTSVDKANVLASSKLWRKTVNEIATEYPEVTVDHLYVDAASMKIIQDPTSFDV
                     IVTENMFGDILSDEASVITGSLGMLPSGSHAVSGPSLYEPIHGSAPDIAGKNIANPMS
                     MILSVAMMLRQSFQLHEEAAALEKAASEVMDAGFLTADLGGTTTTTAFTEQVKAILEK
                     "
     gene            848..2236
                     /gene="leuC"
                     /locus_tag="PLT73_06060"
     CDS             848..2236
                     /gene="leuC"
                     /locus_tag="PLT73_06060"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM19978.1"
                     /translation="MSRTLFDKLWDRHVITGPEGEPQLLYVDLHLIHEVTSPQGFDGL
                     RETNRPVRRPDKTIATVDHNVPTEDIYNIKDLISKKQIEALQKNCAEFNIPLMDIGTA
                     NQGIVHMVGPELGATQPGKIVVCGDSHTATHGAFGAMAFGIGSSEVEHVFATQSIWQK
                     KPKSMGVKITGKLPAGVYAKDIILHLIATYGAAFGSGYAIEFYGDTVEALTMEERMTI
                     CNMSIEFGAKIGMMAPDQTTYDYLRGRRYAPENMEKAIADWETLKSDPDAVYDTDIAI
                     DVSDLAPYVTWGTNPGMGVQFGEKFPEIQDKNDERAYNYMDLKPGQTAEDIPLGFVFI
                     GSCTNARLSDLIEAAKYVKGGQVPAHIQAMVVPGSRTVRDAAAELGLDKIFIDAGFEW
                     REPGCSACLGMNPDKVPAGVHCASTSNRNFEGRQGKGSRTHLVSPAMAGAAAVNGKFV
                     DIRKEAVTYGAN"
     gene            2223..2810
                     /gene="leuD"
                     /locus_tag="PLT73_06065"
     CDS             2223..2810
                     /gene="leuD"
                     /locus_tag="PLT73_06065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRM19979.1"
                     /translation="MEPIKVHNGKTVALMNNNIDTDQIIPKVFLKRIEKTGFGAFVFD
                     EWRFLKNGDPNPEFPLNDADRQEATILVTGDNFGCGSSREHAAWALKDYRFRVIIAGS
                     YSDIFYMNSLKNGLLLIELPQEQRDALAQLPADETIQIDLPNQVVRTNAAEYPFAIDP
                     TWKHKLENGIDDITETMTYADQIDAYEAKWDNIYA"
BASE COUNT          812 a          685 c          776 g          645 t
ORIGIN      
        1 cgcaatcggc ggaccgaaat gggaaaaagc cgagaaaaca ccggaagatg gcctgttgca
       61 attacgtaag acattgggac ttttctcgaa catccgtccg attgccgtca gcgacagcat
      121 cgcccacctc tcccctttga agacggagcg ggtaaaaggc acagacttca tcgtggtccg
      181 tgaattgact ggcggcctgt acttcggcca gccgaagcat tggaacgatg aggaagcgac
      241 tgatacttta ttctataaaa agagcgaaat cgatctgatt gtccgccagg cttttgacat
      301 cgcgatgacg cgcggcaaaa aactgacttc cgtcgataaa gccaacgtat tggccagcag
      361 caaattatgg cgcaaaaccg tgaatgaaat cgcgacagaa tatccggaag tgaccgtgga
      421 tcacttgtat gtcgatgcgg cttcgatgaa aattatccaa gatccgacct cattcgatgt
      481 catcgtgacg gaaaacatgt tcggggacat cctgagcgac gaagcatccg tcatcacagg
      541 ctcactgggg atgttgcctt ccggaagcca tgcggtatcc ggtccttcgt tatatgaacc
      601 gatccatgga tccgcaccgg atatcgccgg taaaaacatc gccaacccga tgtcgatgat
      661 cctatccgta gccatgatgc tgcgccaaag cttccagctg catgaagagg ctgctgcgct
      721 tgaaaaagcg gcctccgagg tgatggatgc cgggttcctg acggccgatc tgggcggcac
      781 gacgacaacg acagcattta ctgaacaagt gaaagccatt ttggaaaagt gaaggggagt
      841 agatagtatg agcagaacgc tttttgataa actatgggac agacacgtca ttacaggacc
      901 agaaggtgaa ccacaattgt tgtacgtgga cctgcacctg atccatgaag tcacttcgcc
      961 acaaggcttt gatggcttgc gcgaaaccaa ccgcccagtc cgacgtcctg acaaaacgat
     1021 cgcaacggtc gaccataatg tgccgacgga agacatctac aatattaaag acctgatttc
     1081 taagaaacag attgaagcgc tgcaaaagaa ttgcgcagaa ttcaacattc cattaatgga
     1141 tatcggaaca gccaatcaag ggattgtgca catggtagga ccggaattgg gcgcaaccca
     1201 acctggtaag attgtcgtct gcggagattc acatacggct acgcatgggg ccttcggagc
     1261 gatggcattc ggtatcggca gctcggaagt ggagcatgtt tttgcaacgc aatcaatctg
     1321 gcagaaaaaa ccgaaatcga tgggcgtaaa aatcaccggc aaattgcctg cgggcgtcta
     1381 tgcgaaagat atcatcctac atttgattgc gacttacgga gcagccttcg gaagtggtta
     1441 tgcgattgaa ttttacggag atacagtgga agctttgacg atggaagaac ggatgacaat
     1501 ctgtaatatg tccatcgagt tcggtgcaaa aatcggcatg atggcaccgg accagactac
     1561 ttacgattac ttgcgcggtc gccgttatgc accggaaaac atggagaaag ccattgccga
     1621 ttgggagacg ctgaagagcg atccggatgc tgtctatgac actgacatcg ctatcgatgt
     1681 ttccgacttg gctccttatg taacttgggg aaccaatcca ggtatgggtg tgcagttcgg
     1741 agaaaaattc ccggaaatcc aggacaaaaa tgatgagcgt gcctataact acatggatct
     1801 gaaacctggt caaacggcgg aagatattcc attgggcttt gtcttcatcg gctcttgtac
     1861 aaatgcccgc ctgtccgatc tgatcgaagc ggcgaagtat gtcaaaggcg gacaagtacc
     1921 tgcccatatc caagcgatgg ttgtaccggg cagccggacc gtccgcgatg cggccgcgga
     1981 attgggattg gacaaaattt tcatcgatgc gggatttgaa tggcgcgagc caggatgctc
     2041 ggcttgcttg gggatgaacc cggataaagt gcctgctggc gtacactgtg cttcgacttc
     2101 caaccgtaac tttgaaggcc gtcaaggcaa aggttcgcgg acgcacttgg tcagcccggc
     2161 aatggcagga gctgccgcag tgaacggtaa atttgtggat atcagaaagg aagcagtgac
     2221 ttatggagcc aattaaagta cataacggta agaccgttgc tttgatgaac aacaacatcg
     2281 atactgacca aatcattccg aaagtattcc tgaaacggat cgaaaaaacg ggtttcggcg
     2341 ctttcgtttt tgatgaatgg cgtttcttga aaaatggcga tccgaatccg gagttccctt
     2401 tgaacgatgc cgaccgccaa gaagcgacaa tcctcgtaac gggagataat ttcggctgtg
     2461 gctcttcccg tgaacatgcc gcatgggcat tgaaggatta ccgcttccga gtcatcatcg
     2521 ccggcagtta cagcgatatc ttctatatga attccttgaa gaatggtctt ttgctgatcg
     2581 agttgccgca agagcaacgt gatgcgttgg cgcaattgcc ggcggatgaa accatccaga
     2641 tcgacttgcc gaatcaagtg gtcagaacca acgcagccga gtatccgttc gccatcgatc
     2701 cgacttggaa gcataaattg gaaaacggca tcgacgacat caccgaaacg atgacttatg
     2761 ctgatcagat tgatgcgtac gaagcaaaat gggacaatat atacgcatag atataaaagt
     2821 ggaacggatt cgttcagccc tgaaagaaat ttaggaaatc ggaccgactg agcatcgtag
     2881 agcgcaatat gctgagagac ttcctgcgtc agcaggtt
//
LOCUS       DAOMUJ010000215         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9806_length_2899_cov_2.684248, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000215 DAOMUJ010000000
VERSION     DAOMUJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_2899_cov_2.684248"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLT73_06070"
     CDS             complement(<1..480)
                     /locus_tag="PLT73_06070"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM19980.1"
                     /translation="MATFYVPAINLIGKGCIKELGSNVKELGYKKALFVTDNFLAKSE
                     MIDVVLAELDNAEIDYVIYADVDPNPTCKNVNEGVAMAQAENCDFIISFGGGSPQDAA
                     SAISIILTNGGKPQDYEGLHKSAKAGLPVVAINTTAGTSAEITINYVITDEDRKVKMV
                     "
     gene            complement(516..1340)
                     /gene="rhaD"
                     /locus_tag="PLT73_06075"
     CDS             complement(516..1340)
                     /gene="rhaD"
                     /locus_tag="PLT73_06075"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HRM19981.1"
                     /translation="MKMKNILEAPFIKEVMLLTDVMYKFGWHERNSGNLSYLLKEEEL
                     TEYLDLNEVKRNIPIAFDGKALAGKYFLVTGTGKFFKNVIHDPADVLGILKVTAEGNS
                     VDLLWGYENGAAPTSELPAHLMSHIARLSVDPENRVVYHCHATNLLAMSFSCELDERS
                     FTRILWKMCTESLVVFPEGVGILPWLMPGTNEIGEATAEKMKEYRLIVWPHHGLYAAG
                     KDLEECFGLVETAEKSATVYTLVQSQGGIRQEITDEQLSNLGKRFSVTPRAGYLNI"
     gene            complement(1351..2607)
                     /locus_tag="PLT73_06080"
     CDS             complement(1351..2607)
                     /locus_tag="PLT73_06080"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRM19982.1"
                     /translation="MTKPIEEAYALAKQKYAAIGVDTDAVLEKLSQIKVSLQCWQGDD
                     VLGFMFPDQALTGGISVSGNYPGKATTPAQLRADLDKALSLIPGNHKVNLHAIYVDTD
                     EKIDLDQIEPKHYEKWVQWAKEKGLGLDFNPTCFSHPKSTDGTLSSNDPETQAFWIEH
                     VKHSRKVAAYFGEELNQTCVNNIWIPDGYKDNPIDKMSPRVRLRDALDQCLEEKFDDA
                     YMLDAVEGKLFGIGAESFTTGSNDFYLSYALTRDILWTIDAGHFHPTEDVSDKFTAFL
                     PFGKGLMLHVSRPIRWDSDHVVILDEATTRIGETLVRNDLLDKTFIGMDFFDATINRV
                     AAMVIGARSTLKSLLLGMLSPIETLKDAESTGDFTTRLAVTEEMKSYPFGAVWEFYCQ
                     QQNVPAGTEWLTEVKDYEQKILTERK"
     gene            complement(2612..>2899)
                     /locus_tag="PLT73_06085"
     CDS             complement(2612..>2899)
                     /locus_tag="PLT73_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM19983.1"
                     /translation="LTKETIDVLHIVGGGANVKLLNQLTADVTGKLVVAGPTEGTAIG
                     NLLVQMIATGEFADLAEARKWLRSQIHVEEYPPNPIADREHLKKYEEKIGV"
BASE COUNT          640 a          731 c          679 g          849 t
ORIGIN      
        1 aaccatcttc actttgcggt cttcatcggt gatgacgtag ttgatcgtga tttcagcgga
       61 tgttccggca gtcgtgttga tcgccacaac cggcaagccg gctttagcgg atttgtgcaa
      121 gccttcgtaa tcctgcggtt tgccgccgtt cgtcaggatg atgctgatgg cgctcgctgc
      181 atcctgagga gagccgccgc cgaaggaaat gatgaagtcg cagttttccg cttgtgccat
      241 cgccacgcct tcgtttacgt ttttgcaggt cgggttcgga tcgacatccg catagatcac
      301 atagtcgatt tccgcattgt ccaactctgc caaaacaacg tcgatcattt cgcttttggc
      361 caaaaagtta tccgttacga acaaagcttt cttgtagcca agttccttca cattgctgcc
      421 caattccttg atgcagcctt ttccgataag gtttattgct ggtacataaa aagttgccat
      481 tttccattat ctccttttgt taaaaatcag atggattaga tattcaagta gcccgctctt
      541 ggggttaccg agaaacgttt gccaagattg ctcagttgtt cgtcagtgat ttcctgtctg
      601 atgccgcctt gcgattgaac aagtgtgtag acagtcgctg atttttcagc cgtttcgacc
      661 aaaccgaagc actcttccaa atcttttccg gctgcgtaca aaccgtggtg cggccatacg
      721 atcagacggt attccttcat tttttcagca gtcgcttcgc cgatttcgtt cgttccaggc
      781 atcagccaag gcaagatgcc cacaccttct gggaacacga ccaatgactc ggtgcacatt
      841 ttccaaagga tgcgcgtgaa gcttctttca tccaactcgc aagagaagga catcgccaac
      901 aggttggtgg cgtggcagtg gtaaacgaca cggttttcag gatcgactga caagcgcgca
      961 atgtgcgaca tcaaatgagc aggcaattcg cttgtaggag ccgctccatt ttcgtatccc
     1021 cataacaggt ctacgctgtt gccttccgca gttactttca ggatacccaa gacatcagcc
     1081 ggatcatgga tgacgttttt gaagaatttc cctgttccgg tgaccaggaa atatttcccg
     1141 gccaaagctt ttccgtcgaa agcgatcgga atgttgcgtt tcacttcgtt cagatccaga
     1201 tattccgtaa gttcctcttc tttcagcaag taggacaagt tgccgctgtt tctttcgtgc
     1261 cagccgaatt tgtacatgac gtccgtcaac agcatgactt ctttgatgaa tggtgcttct
     1321 aaaatgtttt tcatttttat gatttccccc ttatttgcgt tccgtcagaa ttttttgttc
     1381 gtagtctttt acttcagtca gccattccgt accagctggt acattttgtt gttggcaata
     1441 gaattcccag acagcgccga atggataaga tttcatttct tccgtgacag ccaaacgggt
     1501 tgtgaagtcg ccagtgcttt cggcatcctt caatgtttcg atcgggctca acatgcccaa
     1561 cagcaaggat ttcaatgtgc tgcgtgcgcc gatgaccatg gctgcgacac ggttgatcgt
     1621 tgcatcgaag aagtccatgc cgatgaaagt tttgtccaac aagtcgttgc gcaccaatgt
     1681 ttcgccgatg cgggtagtcg cttcatccaa gatgaccacg tgatcggaat cccaacggat
     1741 cggacggctc acgtgcagca tcaagccttt accgaaaggc aggaaggccg tgaatttatc
     1801 ggatacatcc tcggttggat ggaaatgtcc cgcatcgatt gtccacagga tatcccttgt
     1861 caaagcgtag gacaggtaga aatcgttgga accggtcgtg aagctttctg caccgatgcc
     1921 gaagagtttg ccttcgactg cgtccagcat gtacgcatca tcgaattttt cttccaaaca
     1981 ttgatccaaa gcatcacgca ggcgtacgcg cggcgacatt ttgtcgattg gattgtcttt
     2041 gtagccatcc gggatccaga tgttgttcac gcaagtttgg ttcagttctt cgccgaagta
     2101 agcggccact ttgcggctgt gttttacgtg ttcgatccaa aatgcttgtg tttcaggatc
     2161 attactggac aacgtaccat cagttgattt cggatgagag aaacaagtag gattgaaatc
     2221 caggccaaga cctttttctt ttgcccattg cacccatttt tcgtagtgct tcggttcaat
     2281 ctggtccaag tcgatttttt cgtccgtatc cacgtagatg gcatgcagat taactttatg
     2341 attaccaggg atcaggctta aggctttatc caaatcggca cgcaattgcg ctggcgtcgt
     2401 tgctttgcca gggtagttgc cgctgacgga aatcccgccg gtcaaggctt gatcggggaa
     2461 catgaatccc aatacgtcgt ccccttgcca gcactgcaag gaaaccttga tttggcttaa
     2521 tttttcaagt actgcatccg tatccacacc gatggcggca tatttctgtt ttgctaaagc
     2581 gtatgcttct tcaataggtt ttgtcatttg tctatactcc aatcttttct tcgtattttt
     2641 ttaggtgctc ccggtctgcg atcgggtttg gcgggtattc ttcaacatgg atctgacttc
     2701 tcagccattt tctggcttcc gccaggtcag caaattcgcc ggttgcgatc atctgcacaa
     2761 gcagatttcc gattgcggtg ccttcggtag gtccggcaac gaccagcttc ccggtgacat
     2821 cggcagtcaa ctgattcagc aatttaacat tcgctccgcc gccgacgatg tgcaacacat
     2881 ctatcgtttc cttcgttag
//
LOCUS       DAOMUJ010000216         2887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9866_length_2887_cov_2.717867, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000216 DAOMUJ010000000
VERSION     DAOMUJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9866_length_2887_cov_2.717867"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLT73_06090"
     CDS             <1..964
                     /locus_tag="PLT73_06090"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRM19984.1"
                     /translation="EEMTKSADGLTIGKQRVPLGVIGIIYESRPNVTADASALCFKSG
                     NAVILRGGKEALESNKAIMTALQSGLADAGFAKETLQLIPDPSRELASEFMKMNGYLD
                     CLIPRGGAGLIQNVLKNATVPVIETGVGNCHLYIDKDAELEMATRILVNAKCSRPSVC
                     NSIETLVVHEAIANAFLPVFAEALAPYKVELRGDAKTCSILSEAVPATEEDFAAEFSD
                     FILAVKVVSSYDDAIAHIQKYSTGHSEVIVTDHYQTAQNFLNDIDSAAVYINASSRFT
                     DGGCFGFGGEIGISTQKLHARGPMGLKELTSYKYIIYGEGQIRS"
     gene            1069..2199
                     /locus_tag="PLT73_06095"
     CDS             1069..2199
                     /locus_tag="PLT73_06095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM19985.1"
                     /translation="MKKKIAVIGGGIVGATASYYSAKAGYSVSVFDSGTGQATAAAAG
                     IICPWLSRRRNKKWYRLVSEGAAFYDKLMADLASDGLETDAYARCGALILGQSVEYLQ
                     EVHDRALERRKQAPLIGNVAILKGTEIKKIFPPLSNVEQALYVGGGARVDGRELTNTL
                     LQATTRFGGSIHKGTVSLSKDNDGTLSVLTPDGRQQKFDAVILAAGAWLPELLQPLGY
                     TVDVRGQKGQLVVLQTEDSALQDCPVIMPQGEIDLLPFGQGKTVVGASHENDKGYDLQ
                     PDASITDPMLEQAYAWLPDLKEAPVADIRVGTRAYTSDFSPFFGSVPGLDNCFAASGL
                     GSSGLTSGPLIGYLLTELVREQQSTLPLEDYPIDAYIKQTTC"
     gene            2288..2683
                     /gene="mscL"
                     /locus_tag="PLT73_06100"
     CDS             2288..2683
                     /gene="mscL"
                     /locus_tag="PLT73_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRM19986.1"
                     /translation="MWKEFKTFIMRGNVLDLAVGVVIGGAFTAIVNSLVKDIITPIIV
                     AVTGNADLTGLSFKIGQADFTYGNFLQAVLNFLLIALVLFLMIKVINKLKRKMPEEEP
                     VEVEAEVPVVELYLKEIRDLMVDQNKKAE"
BASE COUNT          763 a          754 c          713 g          657 t
ORIGIN      
        1 cgaagaaatg acgaaatcag ccgatggtct gacgataggc aagcaacgtg ttccgcttgg
       61 cgttatcgga atcatttacg aatcgcgccc aaacgtcacg gcggatgcga gtgccctttg
      121 ctttaaatcc gggaatgctg tcatcctgcg cggtggaaaa gaggccttgg aatccaacaa
      181 agccatcatg accgcattgc agtccggcct cgcggacgcc ggcttcgcca aggaaacgct
      241 gcaactgatc cctgatccgt cccgcgaatt ggctagcgaa ttcatgaaaa tgaatggcta
      301 tctggattgc ctgatccctc gtggtggagc cggcctgatc cagaacgtac tgaaaaacgc
      361 gacagtgcca gtcatcgaaa caggcgtcgg aaattgccac ttgtacatcg ataaagatgc
      421 cgaattagaa atggctacgc gcattctggt gaatgcgaaa tgttcccgcc cttccgtctg
      481 caattccatc gagacgcttg ttgtccacga agcaatcgcg aatgctttcc tgcctgtttt
      541 cgcggaagct ttggcgcctt ataaagtgga attgcgcggc gatgcgaaga cttgcagcat
      601 cctgtctgaa gctgttccag caacagaaga agatttcgct gccgaattct cagatttcat
      661 tttggcagtc aaagtggttt cctcctatga cgatgccatc gcgcatatcc aaaaatacag
      721 cacaggccac tccgaagtga tcgtcaccga tcattaccaa acggcacaga acttcctgaa
      781 cgatatcgat tcagcggcag tctacatcaa cgcctcttct cgtttcacgg acggcggttg
      841 tttcggcttc ggtggagaaa tcggcatcag tacgcaaaaa ctccatgccc gtggaccgat
      901 ggggttgaag gaattgactt cctataagta catcatctac ggtgaaggcc aaatcaggtc
      961 ctaagaagaa tcctcatcaa aaatatcgag agccgggaca ccgttcccgg ctttttctgt
     1021 ccatcagcag caaacaccgt cttacagaga aaaggaatga ttacaccaat gaaaaagaag
     1081 attgcagtca tcggtggcgg catcgtcggc gccaccgcaa gttactactc ggcaaaagcc
     1141 ggctacagcg tcagcgtgtt cgacagcggt accggccaag caacagccgc agcggcagga
     1201 atcatttgcc cttggctgtc cagacgccgc aacaaaaagt ggtaccgcct cgtctccgaa
     1261 ggcgcggctt tttatgacaa actgatggcc gacttggctt ccgacggctt ggagacggat
     1321 gcctacgcac gttgcggcgc cctgatccta ggacaatccg tcgaatacct ccaggaggtc
     1381 catgaccgtg cactggaacg tcgcaagcag gcacctctga tcgggaacgt cgctatcctc
     1441 aaaggcaccg aaatcaaaaa aatcttcccg ccgctcagca acgtcgaaca agccctttac
     1501 gtgggcggcg gcgcccgcgt ggacggtcgg gaattgacga atacattgct gcaggcgaca
     1561 acccgcttcg gaggcagtat ccacaaaggg accgtttcac tctccaagga taacgatggc
     1621 accttgtccg tcttgacacc ggacggaaga cagcaaaaat tcgatgcagt catcctggcg
     1681 gcaggcgctt ggctacccga gctactccag ccgctcgggt acacagtcga cgtccgcgga
     1741 caaaaagggc agctggttgt cctccaaact gaagacagcg ctctgcagga ctgcccggtc
     1801 attatgcctc aaggtgaaat cgatctgctt ccgtttggcc aagggaaaac agtcgtcggc
     1861 gcaagccatg aaaacgacaa aggctatgat ctacagcccg atgcttccat tacggatccg
     1921 atgctggaac aagcctatgc ttggctccct gacctaaaag aagcgcccgt cgcggatatc
     1981 cgtgtcggta cgcgggccta cacctccgat ttcagcccct ttttcggtag tgtcccgggt
     2041 ctggataact gcttcgcggc aagcggtctc ggctcatccg ggttgacttc cggcccattg
     2101 atcgggtatt tattgaccga actggtccgt gagcaacaaa gcaccttgcc cctcgaagac
     2161 tatccgattg acgcttacat caaacagacg acttgctaaa aacacagaaa tattatccat
     2221 tatactccgt attttttgcc atttcgtcga acttgaaata cacttatatt agatggaggt
     2281 gaaaaatatg tggaaagaat ttaaaacatt catcatgcgt gggaatgtgc ttgatcttgc
     2341 cgtcggggtt gtcatcgggg gtgcatttac agcaatcgtg aattcattag tcaaagatat
     2401 cattacaccg atcatcgtag ccgtaaccgg taacgctgac ttgacaggcc tttcattcaa
     2461 gatcggccag gccgacttca cgtacggtaa ttttttgcaa gccgtactga acttcctgct
     2521 gatcgccctt gtcctcttcc tgatgataaa ggtaatcaac aaactcaaac gcaaaatgcc
     2581 tgaggaagaa cccgtggaag ttgaagcaga agtaccggtt gttgaacttt atctgaaaga
     2641 aatccgtgat ttgatggttg atcagaacaa aaaagccgaa taagagcgga gtgagcgttg
     2701 gatgatcgag catccaacgt ttttttgtcc caaaacagac atgcaaagat atgttctaag
     2761 taaatagtct gatttctccc aaagttcaca atcactatat ttttatgagg agcattatga
     2821 tatcctatcc ataaaaggaa gtggatagat aatgcccatc aaagcaatcg tttgcgatat
     2881 ggacggc
//
LOCUS       DAOMUJ010000217         2884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9879_length_2884_cov_1.975963, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000217 DAOMUJ010000000
VERSION     DAOMUJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9879_length_2884_cov_1.975963"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLT73_06105"
     CDS             <1..838
                     /locus_tag="PLT73_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM19987.1"
                     /translation="EIAVQALEAGKHVICEKPVTLTSEELSEIMHTAKEHERVFMVHQ
                     NRRWDSDFQMVKDLYENRKIGEVFHIESRVQGANGIPGDWRHLKAHGGGMLLDWGVHL
                     LDQLLYMIDSPLAKVAADLSYILGDEVDDGFISYLTFENGVTALVEVGTTNYIKLPRW
                     YIKGTKGSAVIEDWDLKGKIVKQSGIELAAAPKPIKAGQGLTKTMAPPSEEAIIKEKF
                     EKVATQMDPFYTNFAKVVRGESEPIVKNEEVLQLMKIIESIFEAAETGQVIDVREHSY
                     SR"
     gene            930..1904
                     /locus_tag="PLT73_06110"
     CDS             930..1904
                     /locus_tag="PLT73_06110"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HRM19988.1"
                     /translation="MQKKVLGIDLGGTSVKLAILSKEGDVLQKWSIPTDNSDGGVKIV
                     PGIIESIKHHLDLYRLTSEDFLGIGMGSPGAVNRSEGTVTGAFNLNWRDVQPVRETME
                     QETGIPFIIDNDANVAALGEKWRGAGEDNQDVVFITLGTGVGGGIIAEGQLIHGTAGS
                     GGEIGHMTVDPDGFDCTCGKRGCLETVASATGVVKLARKHAEEYAGDSTLKNLIDDGQ
                     EITSKMIFDFAKDDDGLAVIVVDRFAYFLGLACSHIGNLLNPASIIIGGGVSAAGEFL
                     LEKVNKYYLGFAFPNVQQSTKLKLAELGNDAGIIGACYLAVAHASKYQ"
     gene            complement(1998..2621)
                     /locus_tag="PLT73_06115"
     CDS             complement(1998..2621)
                     /locus_tag="PLT73_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM19989.1"
                     /translation="MRNEIIIEDVKHIVAEKLRNEATGHDWWHTLRVYNTSMDIAAAE
                     GKGDRLIIGLAALLHDVADHKFGYTDADREAIITDILTPFELSDSVIEQVIYIVNNLS
                     FKQGKNKHVLETIEGRIVQDADRLDAIGAIGIARAFAYGGHKDRPLYDPEHSEDPDSD
                     TIGHFHEKLLLLKDGMHTEAGKQKAVARHTRMEQFLKDFHSEWDGHQ"
     gene            complement(2659..>2884)
                     /locus_tag="PLT73_06120"
     CDS             complement(2659..>2884)
                     /locus_tag="PLT73_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HRM19990.1"
                     /translation="QDYGLASLALPKGLIVLHHKGFAYDNSNMERLLEDRYMSAMVRK
                     SGKRTKGPKPFTDADRHKFRKLLISKLQET"
BASE COUNT          741 a          616 c          793 g          734 t
ORIGIN      
        1 agagatcgct gtccaggcgt tggaggcagg aaaacatgtc atttgtgaaa agcccgtgac
       61 actgaccagc gaagagctgt cggaaataat gcataccgca aaggaacacg agcgcgtgtt
      121 tatggtccat caaaatcgcc gttgggacag tgattttcag atggtcaaag atttgtacga
      181 gaaccgaaaa atcggagagg tcttccacat cgaatcccgt gttcagggag ccaatggtat
      241 accgggtgac tggcgccatc tgaaagcgca cggtggcggc atgctcttgg attggggggt
      301 tcatttgttg gatcagcttt tgtatatgat cgacagcccg ttggcgaaag ttgctgcaga
      361 tttgagttac atattgggcg atgaagtgga cgacggcttc atcagctatc tgactttcga
      421 aaatggcgta accgcccttg tagaagtcgg gacgaccaat tatatcaaac tcccgcgttg
      481 gtacataaaa gggacaaaag gatcagcggt gatagaagat tgggatctaa aaggaaaaat
      541 cgtgaagcag agtggaattg aacttgcggc agcgcctaaa ccgataaaag ctggccaagg
      601 gctgaccaaa acgatggctc cgccttccga agaggcgatc ataaaagaaa aatttgaaaa
      661 agtggcaact caaatggatc cgttctatac gaatttcgct aaagtcgtca gaggcgagtc
      721 ggaacctatc gtcaaaaatg aagaagttct tcaattgatg aaaatcattg aatccatttt
      781 tgaggcagcg gagacaggac aagttattga cgtacgggaa cacagctaca gccgttaaaa
      841 acagctggac cgttgcattc atattttgaa aatgtgagca attaagcatc gtaaacggtg
      901 aaaaagtttt gatttggagg caatacgaga tgcagaaaaa agtattggga atagatttgg
      961 gtggaacttc agtgaagttg gccatattat ccaaagaagg ggacgtgctc cagaaatgga
     1021 gtatcccgac agacaacagt gacggcgggg ttaaaatcgt gccgggaatt atcgaatcca
     1081 tcaaacatca tttggactta taccgactca cttctgaaga ctttttgggc ataggcatgg
     1141 gttctcctgg tgcagtcaac agaagcgaag ggaccgttac gggagcattt aacctgaatt
     1201 ggagagatgt gcaaccggta agggagacaa tggaacaaga aacgggcata ccatttatca
     1261 tcgacaatga tgccaatgta gctgcgctgg gagaaaaatg gcgtggtgcg ggcgaggaca
     1321 atcaggatgt cgtttttatc accttaggga caggcgtcgg cggagggatc attgctgagg
     1381 gccaactgat ccacggaaca gccggctcgg gcggagaaat cgggcatatg acggttgatc
     1441 ctgatggttt tgactgcact tgcgggaaaa ggggctgttt ggagaccgtt gccagcgcta
     1501 ccggggtcgt caagctagcg cgcaaacatg cagaggaata tgccggagat tcaaccttaa
     1561 aaaatctgat cgacgatggc caggaaatca cttcaaaaat gattttcgat ttcgctaaag
     1621 atgacgatgg attagcggtt atcgttgtgg atcgctttgc ctatttttta ggtctggcat
     1681 gcagccatat cggtaatctg ttgaatccgg cttccatcat catcggtggc ggcgtttctg
     1741 ctgcagggga atttttgctt gaaaaagtca ataaatacta tttgggattt gctttcccga
     1801 atgtacagca atcgactaaa ttgaagcttg cagaattggg gaacgatgct ggaattatcg
     1861 gggcctgtta tttagcagtg gctcatgcga gtaagtatca ataacttgct ttattttcag
     1921 attactaatg ccattttaat ggaaaagagt ggctccaaat cattcgaact gatttggagc
     1981 cactcttatt tgtgttgtta ctggtggcca tcccattcgg aatggaagtc cttgaggaac
     2041 tgttccatcc gagtgtggcg ggcgaccgcc ttttgtttgc cggcttccgt gtgcatgccg
     2101 tccttcagca ggagcagttt ttcgtggaag tggccaatcg tatcgctgtc cggatcctca
     2161 ctgtgctcgg gatcatagag aggccggtct ttatgcccgc catatgcaaa agcgcgtgcg
     2221 atgccgattg cgccgatcgc atccaggcga tcagcatcct gcacgatccg gccttcgatc
     2281 gtttccagca cgtgcttgtt tttcccttgc ttgaaggaaa ggttgttcac gatgtagatg
     2341 acctgctcga tgacggagtc gctcagctcg aaaggtgtga ggatgtcggt gatgatagct
     2401 tcgcggtcgg cgtcagtata gccgaattta tggtcggcaa cgtcatggag aagtgccgcc
     2461 aagccgatga tgagccgatc gccttttccc tccgctgcag cgatgtccat ggacgtgttg
     2521 tagacccgca acgtatgcca ccaatcatgc cccgttgctt cgttcctcag cttttcagcg
     2581 acgatgtgtt tcacatcttc gataatgatt tcattgcgca tccagatgca cctctttctt
     2641 gattctgtta ggattccatt aggtttcttg cagcttggaa atgagcagtt tccggaattt
     2701 gtgccgatcc gcatccgtga atggtttcgg gccttttgtg cgtttgccgc ttttgcggac
     2761 catggcgctc atgtagcggt cttcaagcag ccgttccata ttgctgttgt cgtaggcgaa
     2821 ccccttgtga tggaggacga tgagcccctt cggcaaagca agggaggcca aaccgtagtc
     2881 ctgc
//
LOCUS       DAOMUJ010000218         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9926_length_2874_cov_2.433842, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000218 DAOMUJ010000000
VERSION     DAOMUJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_2874_cov_2.433842"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="tkt"
                     /locus_tag="PLT73_06125"
     CDS             <1..1894
                     /gene="tkt"
                     /locus_tag="PLT73_06125"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM19991.1"
                     /translation="MAYALWANHLKVNPKNSLWADRDRFILSAGHGSAMLYSLLHLAG
                     FAVTIDDVKNFRQYESKTPGHPEVHYTDGVEATTGPLGQGIANAVGFAMAEAHLAATY
                     NKENFPVVDHYTYFLNGDGDLMEGISHEAASLAGHLKLGKLIGLYDSNDISLDGPTSK
                     SFTEDVAARFEAYGWQHILVKDGNNLEAISKAIEEAKAETEKPTLIEIKTIIGFGAPD
                     AGTHKVHGAPLGAEGVAFAKAAYGCPDEAFCVPAEVSVRFNEKMVEAGQQAEAAWTAL
                     FNDYKAAYPELAQQFEDAMAGKLPEGWQDKLPTYEVGSAAKASRVTSAEAIQALGETV
                     PYFWGGSADLSSSNNTMIKSASDFEPGNYAGRNIWYGVREFAMAAIMNGIVLHGGTRT
                     YVGTFFVFTDYLRPAMRLAAISHLPGTYVMTHDSIAVGEDGPTHEPVEHLSSYRGMPN
                     VTVLRPADGNEVSAAWEIAVSSADKPTMLVLTRQNLPVLEGTKEMAREGVKKGAYVLS
                     PQQGETPAGILIATGSEVNLAVEAQQQLREAGVDVSVVSMPSFDLFEAQDAAYKETVL
                     PGAVRNRMSIEMGATFGWERYVGLDGLAYGIDTYGASGNGNVVMAEYGFTTEKVVAAY
                     QAKFAK"
     gene            complement(2269..>2874)
                     /locus_tag="PLT73_06130"
     CDS             complement(2269..>2874)
                     /locus_tag="PLT73_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM19992.1"
                     /translation="DTAIPIGHYTDYHVTPTCGLADSKDFIGQVDEPKYFMDSGRMDA
                     SILWFTAGYVEYKTPNFLTPDDTLEQIDISMEISSEFPFSNDVWPSDITFTLNGTELG
                     TWTSPGDFADTRGKLNPSWWPSNLNQYGLLKTLRVTHHGTYMDGDPLSQVSISDLNKT
                     AETWEIRIEVKPDAENVGGVTLFGKKFGNHDQDINFKAYYS"
BASE COUNT          695 a          726 c          794 g          659 t
ORIGIN      
        1 gatggcctat gccctttggg cgaaccattt gaaagtcaac cctaaaaaca gtttgtgggc
       61 agaccgcgac cgtttcatcc tttcggccgg gcatggctcc gcgatgctgt acagcttgtt
      121 gcatctggcc ggcttcgctg taacgattga tgacgtgaag aatttccgcc agtatgaaag
      181 caagacaccg ggccacccgg aagtgcacta tacggacggt gtggaagcga caaccggccc
      241 tttgggacaa gggatcgcca atgctgtcgg tttcgcgatg gcggaagcgc acttggcggc
      301 tacctacaac aaagaaaact tcccggttgt ggatcactat acttacttcc tgaacggcga
      361 cggcgatctg atggaaggca tctcccatga agcagccagc ttggccggcc acctgaaatt
      421 gggcaaactg atcggtttgt atgactccaa cgatatctcc ttggatggac cgacttcgaa
      481 atcattcacg gaagatgtag ccgcacgctt cgaagcctac ggttggcaac atatcctggt
      541 caaggacggc aacaatctgg aagccatctc caaagccatt gaagaagcaa aagccgaaac
      601 cgaaaaaccg actttgatcg aaatcaaaac catcatcggc tttggtgctc ctgatgcagg
      661 aacgcataaa gtgcatggcg ctccattggg agcggaaggc gttgccttcg ctaaagcggc
      721 ttacggctgc ccggacgaag ctttctgtgt tcctgctgaa gtgtcggtac gattcaatga
      781 aaaaatggtc gaagcaggcc aacaggctga agcagcctgg acagcactgt tcaacgacta
      841 caaagcagct tatccggaat tggcgcaaca attcgaagac gcgatggcag gcaaattgcc
      901 tgaaggctgg caagacaaat tgccgactta tgaagtgggc agcgcagcca aagctagtcg
      961 tgtgaccagt gcggaagcca tccaagcatt gggtgaaacg gtaccgtatt tctggggcgg
     1021 ttctgcggac ttatcgtcct caaacaacac aatgatcaaa tcggcaagcg actttgagcc
     1081 gggcaactat gccggccgca acatctggta cggtgtgcgc gaattcgcga tggccgccat
     1141 catgaacggc atcgttctgc atggcggaac cagaacgtat gtaggaacct tcttcgtctt
     1201 cacggactac ttgcgtccag ccatgcgttt ggctgccatt tcgcacttgc caggcactta
     1261 cgtgatgacg catgactcaa tcgcggtcgg cgaagacggc ccgacgcatg agccagtgga
     1321 acacttgtcc agctaccgcg gcatgccgaa cgtgacagtt ctgcgtcccg ccgacggcaa
     1381 cgaagtcagt gctgcatggg aaatcgccgt gtcttcagcg gacaaaccaa cgatgttggt
     1441 gctgacgcgc caaaacttgc ctgttttgga aggcacaaaa gaaatggccc gcgaaggcgt
     1501 gaagaagggt gcttacgtac tttctccaca acaaggcgaa acgcctgccg gcatcctgat
     1561 cgcaaccggt tcggaagtga acttggctgt tgaagcgcag caacaattgc gtgaagccgg
     1621 agtggacgtt tctgtcgtct ccatgccgag tttcgatctg ttcgaagcgc aggatgctgc
     1681 ctacaaagaa acagtcttgc ctggcgcagt ccgcaaccgc atgtccatcg aaatgggcgc
     1741 gaccttcggt tgggaacgtt atgttggcct ggacggattg gcttatggca tcgacactta
     1801 cggtgcaagc ggcaacggca acgtggtaat ggcggaatat ggctttacta ctgaaaaagt
     1861 ggttgcagcc tatcaagcga aattcgcaaa atagacttct taatggatga ataaaaaagc
     1921 ttgggctgtc tcgaaattga ggcagcccac gctttttttg tccttgtcca gagtttttgc
     1981 atcggaaatg ccgtttgatc agatcaaacg catcaggatg aaaatgatcc ctgacctccg
     2041 gtaaacaaag gccttaccgg aggtcggaca acatttcggg gtgcgttctc cgataagcgg
     2101 ggactcgccg gggttgcggc ctgaagttac cgttgtgctc cggcgagcgc ggcttcgccg
     2161 gagcagaggc tttccttcga ttgccggtat gcatctccgt caatcgaagg aaagccaaaa
     2221 aagctgcggt ccactcgaaa ggagtgggct gcagttttct ggtgcttatc aggaatagta
     2281 ggctttgaag ttgatgtcct ggtcatgatt cccgaatttt ttgccgaaca gggttacgcc
     2341 tccgacattt tcagcatcgg gcttcacttc gatgcggatt tcccatgttt cggctgtctt
     2401 attcagatcc gatatgctga cttgggaaag cgggtctccg tccatgtaag tgccgtggtg
     2461 ggttaccctg agtgtcttca ataaaccgta ttggttcaaa ttcgagggcc accaagatgg
     2521 attcagcttt ccgcgcgtat ccgcgaagtc gcctgggctc gtccatgtgc ccaactctgt
     2581 tccgttcaag gtgaatgtga tatcggacgg ccaaacatca ttcgaaaaag gaaattcgga
     2641 actgatttcc atgctgatgt caatctgctc cagtgtgtca tcaggcgtca ggaagttggg
     2701 agttttgtat tccacatagc ctgcagtgaa ccaaaggatg ctggcgtcca ttcgcccaga
     2761 gtccatgaag tatttcggtt catccacctg accgatgaaa tctttggaat cagccaagcc
     2821 gcatgtcggt gtgacatgat aatccgtgta gtggccgatt ggaatggcgg tatc
//
LOCUS       DAOMUJ010000219         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_9946_length_2870_cov_3.088099, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000219 DAOMUJ010000000
VERSION     DAOMUJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_2870_cov_3.088099"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="rsmI"
                     /locus_tag="PLT73_06135"
     CDS             <1..839
                     /gene="rsmI"
                     /locus_tag="PLT73_06135"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRM19993.1"
                     /translation="GTLYLIATPIGNLEDMTFRAIKILREVDLIAAEDTRNTRKLLTH
                     FEVDTPQISFHEHNTQERIPQLVDKLLGGQSIAQVSDAGMPSISDPGHDLVRACINAN
                     VPVVPLPGANAGVTALIASGLAPQPFYFFGFLERKKKEQVAQLESLKNREETMIFYES
                     PYRLKELLKNIATVMGAERQVVICRELTKRFEEFIRGSAEEVAAWAEETEIRGEICLM
                     IAGNDNPEMPVEQTFDDLSIAELVEKLMTEQGLSSKDAIKETAKIRDLKKQEVYQAFH
                     GF"
     gene            856..1878
                     /locus_tag="PLT73_06140"
     CDS             856..1878
                     /locus_tag="PLT73_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM19994.1"
                     /translation="MLFYLLSICSFGGILMLRLMGKSRQFQSYLLVAAVLSLFAGMLQ
                     GNESPQIVWIRYAVMLFRLLFGLISPFAFLFFGLALVFNGVLLINKEGWAKRYALLLA
                     VGIFILLMDLLFLLNYFVFHHYLIWRFMRLMSYFIIYFGVVLILFFVATTFYGLIPVS
                     LDKDFIIVLGAGLLPDGRISPLLEKRLDRAIRFFRHQTEHTQKRPCRMIVSGGQGVDE
                     PFSEAYAMKRYLIAKGIPEELIIEEDKSVNTYQNLLYSKGIMDTYKEKYKCLFITNNF
                     HTVRSSLYAHRVGLDTDGLGAWTPFYFLPYAFLREFIAVVFMQIKGHTLLLALVLVFG
                     LWRIYL"
     gene            complement(1961..2851)
                     /locus_tag="PLT73_06145"
     CDS             complement(1961..2851)
                     /locus_tag="PLT73_06145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM19995.1"
                     /translation="MPIKAIVCDMDGTLLTAGHDISPKTQEILLTLQQKGITLVLASG
                     RSYLRLMPDALKLKMDSHQGYLIDVNGSSIYDCTSKVRERLGIMDSEDIRKVTSVFRT
                     FNVEIQFSQDAGLYTYLPDSLYDLKKRIRGEMRLPDDYPWASGMYGWLCDMRDGYPEQ
                     KLILDLKEAPTSCNKISISQDPHFMEAVTQSLPHLGLHDDYELVFSDERKLEITKKGI
                     NKGNALDTLLEKLGLSNEEVAVFGDSENDISMLSGRPYSFAMDNALPKAKSVANYATP
                     SNDDEGVYHGLKQLISEGLL"
BASE COUNT          686 a          632 c          748 g          804 t
ORIGIN      
        1 caggaacatt gtatttgatt gcaacgccaa tcggcaatct ggaggatatg accttccgtg
       61 ccataaagat actgcgcgaa gtggatctga ttgcggcgga ggacacgcgc aatacgcgga
      121 aactgctgac gcatttcgaa gtggatacac cccagatcag cttccatgaa cacaataccc
      181 aggagcgcat tccgcaattg gtggataagc tgcttggtgg acaatcgatc gctcaagtga
      241 gcgatgcggg aatgccttcg atcagcgatc cgggtcatga tttggtgcgc gcctgcatca
      301 acgcaaacgt gccggttgtg ccgttgcctg gagccaatgc cggtgtgacc gcattgatcg
      361 cttctggatt ggcaccacag cccttctact ttttcggctt tttggagcgc aagaagaagg
      421 agcaagtggc gcagttggag tcgttgaaaa acagagaaga gacgatgatt ttctatgagt
      481 cgccttatcg gttgaaagaa ttgctcaaaa atatcgcaac cgtcatgggt gcggagcgcc
      541 aagtcgtcat ctgccgcgaa ttgacgaaac gctttgagga gttcatccgg ggttcggctg
      601 aagaagtggc tgcttgggcc gaagaaacag aaatcagagg ggaaatctgc ctgatgatag
      661 ctggcaatga taatccggaa atgccagttg aacagacatt cgatgacctc agcattgctg
      721 aattggtgga aaagctgatg acagagcagg gactttcttc taaagatgcg atcaaagaga
      781 ccgcaaaaat cagggatctg aagaaacagg aagtgtatca agctttccac ggcttctaaa
      841 caggagggat gaccgttgct gttctattta ttgagtatat gctcgttcgg tgggatcctg
      901 atgctccggt tgatggggaa aagtcgacaa ttccaaagct acttgctggt tgcggcggtg
      961 ctgtccctct ttgcaggaat gctgcaaggc aatgaatcgc cccaaatagt atggatccgt
     1021 tacgcggtga tgctatttcg cctcttgttc ggcttgattt caccgtttgc tttcctgttt
     1081 tttggcctgg cgcttgtctt caatggcgtg ttgctgatca ataaagaagg ctgggctaag
     1141 cgttatgcgc ttctgcttgc ggtgggaatc ttcattttgt tgatggatct gctgttcctg
     1201 ctgaactatt ttgtgttcca tcattatctg atttggcgct tcatgcgctt gatgagctac
     1261 ttcattatct atttcggggt agtgctgatc ttattttttg tcgcgacaac cttttacgga
     1321 ctcattccgg tttcccttga taaagatttc atcattgttt tgggcgcagg attgctgccg
     1381 gatggaagaa tcagtccttt gttggagaaa cgcttggaca gagcaatccg gttttttagg
     1441 catcagacgg agcatacgca gaagaggccc tgccggatga ttgtgagcgg ggggcaaggt
     1501 gtggatgaac ccttttccga agcgtatgcc atgaaacgct atctgattgc gaaaggtatc
     1561 ccggaagaac taatcattga ggaagacaag tccgttaata cgtatcagaa tttgctgtac
     1621 tcgaagggca tcatggatac gtacaaagaa aagtacaagt gcctcttcat cacgaataat
     1681 tttcatactg tccgcagcag tctttacgct caccgagtgg gattggatac ggacggtttg
     1741 ggtgcctgga cgccgtttta ttttctgcct tatgcgtttt tgcgtgaatt catcgctgtt
     1801 gtattcatgc agataaaagg acacacgctg ttgctggcac tcgtgctggt gtttggattg
     1861 tggcggatct atttgtgaaa tcataagcgc cgatttgggt gcaaaaagcc ttataccatg
     1921 cgatatttcg caagtatacg gcttttttat gttcgctcgt ttaaagcaat ccttcggaaa
     1981 tcaattgctt caagccatga tagacgcctt cgtcatcatt ggaaggcgtt gcatagtttg
     2041 ccacgctctt ggctttgggc aacgcgttgt ccattgcgaa ggagtagggt ctgccggaaa
     2101 gcatggagat gtcattttcg ctgtcgccaa aaacagccac ttcttcattg gaaaggccga
     2161 gtttttccaa taaggtatcc aaggcattgc ctttattgat gccttttttg gtgatctcaa
     2221 gtttcctctc atcagaaaag accagctcgt aatcgtcatg cagacctaag tgaggcaagg
     2281 attgcgtcac agcctccata aaatgggggt cctggctgat cgaaattttg ttgcaggaag
     2341 ttggcgcttc cttcagatca aggatcagtt tttgttccgg atagccgtcg cgcatatcgc
     2401 acaaccaacc gtacatgccg ctggcccaag ggtaatcatc gggcaatctc atttccccgc
     2461 ggatcctctt tttcaggtca tacagtgaat ccggcaagta ggtgtacagg ccggcatctt
     2521 gggagaattg gatctcaaca ttgaatgtac ggaagacaga tgtgactttt cggatgtcct
     2581 ccgaatccat tatccctaat ctttcccgga ctttagaggt gcaatcataa atggagctgc
     2641 cgtttacgtc gatcaaataa ccttgatggg aatccatctt cagtttcagg gcatccggca
     2701 tcaaacgcaa gtaacttctc ccgcttgcca ataccagcgt aatgcctttt tgctggagtg
     2761 tcagaaggat ttcttgggtt ttggggctga tgtcatggcc agcagtcaat aaggtgccgt
     2821 ccatatcgca aacgattgct ttgatgggca ttatctatcc acttcctttt
//
LOCUS       DAOMUJ010000220         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10012_length_2857_cov_1.714133, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000220 DAOMUJ010000000
VERSION     DAOMUJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10012_length_2857_cov_1.714133"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLT73_06150"
     CDS             <1..985
                     /locus_tag="PLT73_06150"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM19996.1"
                     /translation="RQIAVAAGIPYEKTAMTINEVCGSGLKAIILASQAIRLGDARTV
                     VVGGTESMSQAPYLLPNQRWGSKLGDINTIDSLVHDGLTDAFDQQHMGITAETVTERF
                     QVSREEQDAFALHSQQKAAAAQAAGYFNQEIAPIILIDRKNQQKVVAEDEYIRKDATI
                     ESLGKLRPAFQKNGTVTAGNASGINDGAAALVLMSKSEAEAKGVPYLATIKGYAETGI
                     DPAIMGYAPYYAIKQVLSKTGLLADDIDLFELNEAFASQSIAVIRDLGLPEEKINISG
                     GAIALGHPIGASGSRILVTLLHALERTDTKLGLASLCIGGGMGIAMIVERP"
     gene            1171..2646
                     /gene="thrC"
                     /locus_tag="PLT73_06155"
                     /pseudo
     CDS             1171..2646
                     /gene="thrC"
                     /locus_tag="PLT73_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
BASE COUNT          764 a          748 c          714 g          631 t
ORIGIN      
        1 gaggcagatt gcagtcgctg ccggaatccc ctatgaaaaa acagccatga cgataaacga
       61 agtctgcggc tccggattga aagcaatcat cctggcgagc caagctatcc gtctcggcga
      121 cgcgagaaca gtggtcgtcg gaggaaccga aagcatgtcg caggcccctt atctcttacc
      181 gaaccaacgt tggggcagca aactcggcga catcaacacc atcgacagcc tcgtgcacga
      241 cggcctgacg gatgcctttg atcagcagca tatgggcatc acggccgaaa cggtgacaga
      301 aagattccaa gtgagccgtg aagagcagga cgctttcgcg ctgcattcgc aacaaaaagc
      361 ggccgctgct caagcagccg gctacttcaa tcaagagatt gcgcctatca tcctcataga
      421 ccgcaagaac cagcagaagg tcgtagccga ggatgaatac atccgcaaag acgctactat
      481 cgaaagcttg ggcaaattaa ggccggcctt ccaaaagaac ggcacggtga ctgccggaaa
      541 cgcatccgga atcaatgacg gagcggcagc cttggtgctg atgtcgaagt cggaggccga
      601 agcgaaaggc gtcccttatc tggccacaat caaaggttat gccgaaacag gaatcgatcc
      661 tgccatcatg ggatacgcgc cttactatgc catcaagcaa gtattgtcca aaaccggatt
      721 gcttgcagat gacatcgacc ttttcgaatt gaacgaagcg ttcgcatccc agagcatcgc
      781 cgtaatcagg gatttgggct tgcctgagga aaaaatcaat atcagcggcg gcgccatcgc
      841 cttgggacat cctatcggcg catccggatc tcgcattctg gtgactctgc tgcatgcgtt
      901 ggaacggact gatacgaagc tcggactcgc ctccctttgc atcggtggcg gaatgggcat
      961 cgccatgatc gttgagcgcc cttgaactgt ttttgaccgc acacaatcac tccccgtcat
     1021 ttgattttga caggggagtt ttttcataaa aaagagcgtc agaaaaacgc gcatttcgat
     1081 atgttattga gttttcaaca aaaatgtggc atactcttta tgagaaattt ttaatttaaa
     1141 aatgagaata atgtagagga gactgaatcc gtgacgacct atcatagcac acgaaatgca
     1201 gaaaaaacac tgacggcttc acaagcaata ttacaaggga tagcgccaga cggcggattg
     1261 tacgtacccg atcacattcc ggctttggac atcgcattgg atcgcctgcc cgacatgact
     1321 tatcaagaat tagcttacga tgtgatgaag aaattcttcg acgacttcac tgaggaagaa
     1381 ttgaaacatt gcgtcaatgc agcctacgac gacaaatttg atgactccag catcgttccg
     1441 ttagtgaaag ccggcggaaa ccactatctg gaattgttcc acggaccgac catcgccttc
     1501 aaagacatgg ccctttcgat cctgccttac ttgatgacga cttccgccaa aaagaacggc
     1561 agcacgaaag aaatcgtgat cctgacggcg acttctggtg acaccggaaa agcggcgctt
     1621 gacggattcg ctgatgtgcc gaacacacgc atcatcgtgt tctatcctaa gaacggcgtc
     1681 agcgccatcc aggaaaaaca gatggtgaca caaaaaggcg acaacacctc tgttgtggcc
     1741 atcgacggaa acttcgatga cgcacagacg aacgtcaaaa acatgttcgg aaacgcagac
     1801 ttggcggcac gtttggacgc agcaggattc caattctctt ctgcgaactc catcaacatc
     1861 ggccgtctga ttccgcaagt ggtctattac gtctacgctt acggccaaat ggtgaaacaa
     1921 ggtgccatcg ctgccggaga gccgatgaac gtcgtagttc ctaccggcaa cttcgggaac
     1981 atcttggctg cctacttcgc taagcaaatg ggcttgccga tccaaaaact gatcatcgct
     2041 tcaaacgaga acaaagtctt ggtcgatttc ttcgataccg gcgcatatga tcgcaaccgc
     2101 gacttcattt tgacgacttc cccatccatg gatatcctgg tctccagcaa cttggagcgt
     2161 ctgttgtacc tgatggccga cgaaagtacg gaagcagtcc aaactttcat ggatcaattg
     2221 aaggaaatcg gtcgctacga agtgacggaa gcaatgaaag aaaaagcgaa ggatttctat
     2281 gcggactacg cgactgaaga agaagtggcg gcacagattg ctgcacttta tcatgctgat
     2341 ggctatgtca tcgacccgca tacagctgtg gcggcgcatg tcgcaaacac ttatgtagcc
     2401 gaaaccgatg atcacacacc gatcatcatc gcatcgactg ccagcccgta taaattcccg
     2461 caagccgtcc tgactgccat cgaccctgag ttttcggatg attccatgga agaaatgttg
     2521 gaccgtctga gagaattgag cggcgttgct ttcccagcag ccatcgaaga attattgcat
     2581 gcggaagttc gccacaacac cgttgtcgct tccgacgaca tgcagaagag cgtcgagtcc
     2641 atcctggatt caagaatgtt taaaagccta gccggcggtc cggaatgttt ccgggctgcc
     2701 ggcttttttg cgcgtactgg ctgccttgtc ggctgttcag tattcgtctg tctgcaaaca
     2761 gccgacagat tagattcctc tcggctgttc cgcattcgtc tgtctgcgaa cagccgacag
     2821 attgcatttc tctcgactat tccgtgttcg gctggct
//
LOCUS       DAOMUJ010000221         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10037_length_2852_cov_1.950304, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000221 DAOMUJ010000000
VERSION     DAOMUJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10037_length_2852_cov_1.950304"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="ftsA"
                     /locus_tag="PLT73_06160"
     CDS             <1..903
                     /gene="ftsA"
                     /locus_tag="PLT73_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRM19997.1"
                     /translation="DGIRDPRGMIGVRLELYASMITGPKTIIHNIKRCVEKAGLQIED
                     MVVQPLAISSVAMNKGERDFGTILIDMGGGQTSASVMHDDQLKFAFVDPEGGDLVTKD
                     ISIILNTTLENAERVKREYGYAISEDTSDEEFFPVETIGKEEPVKVDERYLSEIIEAR
                     FVQIFENIKRALDKVEARDLPGGIILTGGAAALPGVVDLAKEIFEINVKLYIPEQMGM
                     RNPIYATSIGLIKYVAGLDDIYRVAKGKVQATGKVIPLQSKTVKQPAVIEEPVYETET
                     YADEENYEESLVGKLKRWFNNLFE"
     gene            949..2238
                     /gene="ftsZ"
                     /locus_tag="PLT73_06165"
     CDS             949..2238
                     /gene="ftsZ"
                     /locus_tag="PLT73_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRM19998.1"
                     /translation="MELEFDSSMNDGAKIKVIGVGGAGSNAVNRMIEEGVQGVEFIVA
                     NTDTQALNASRAETRIQLGPKLTKGLGAGSIPEVGRKAAEESEQQISEALAGADMIFV
                     TCGMGGGTGTGAAPIVARIAKDLGALTVGVVTRPFTFEGPKRGRYAAEGIAELKANVD
                     TLVIISNNRLLEIVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MKDQGSALMGIGIASGENRTAEATKKAISSPLLEVSIDGAEQILLNITGGSDLTLFEA
                     QDASDIVAQASTSEVNIIFGTSINENLGDEVIVTVIATGIDDKKKDEKKTANRGGSAF
                     KGRKEVGNTPVSYPEKQLPTERAEEKPAKDLFGDWDIRRDTSVREQAPKSAQPEPEEY
                     SVKQTEDNYPRYHENKEDRNGGEDSLDTPPFFRKRRR"
     gene            2241..>2852
                     /locus_tag="PLT73_06170"
     CDS             2241..>2852
                     /locus_tag="PLT73_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRM19999.1"
                     /translation="MTIEQNCRKVESTVEAACLTAGRKREEVTVVAVTKTKPVEQVEE
                     LYRLGYRHFAENRPEGLIEKQTALPQEDIYWHYIGTLQTRKVKQVINRIAYFHALDRM
                     SLAKEINERAEKPVSCFVQVNVTGESSKHGLSASEAIPFIKSLEGFPNITVVGLMTMA
                     PIDATDVELRRCFKDLKIIQEQVAALNLAHAPCTETSMGMSQDY"
BASE COUNT          898 a          603 c          725 g          626 t
ORIGIN      
        1 gatggtatca gagatccgag aggcatgatt ggcgtacgtc ttgaattgta cgcaagcatg
       61 atcacaggtc caaaaacaat catccataac atcaaacgtt gtgtagagaa agctggtctt
      121 caaatcgaag atatggtcgt acagccattg gccatttcaa gcgtagccat gaacaaaggc
      181 gagcgggatt tcgggaccat tcttatcgat atgggtggcg gccaaacaag tgcctctgtc
      241 atgcacgacg atcaattgaa atttgcattt gttgatcccg agggtggcga ccttgtgacc
      301 aaggacattt ccatcatttt gaacacgaca ctggaaaatg cggagcgcgt aaaacgcgaa
      361 tatgggtatg ccatttccga agacacgtcc gatgaggaat ttttccctgt cgaaacaatc
      421 ggcaaagaag aaccagtaaa agtcgatgaa agatatctgt ccgaaatcat cgaagcgcgt
      481 tttgtgcaga tttttgaaaa catcaaacgt gccttggata aagtggaagc acgcgatctt
      541 ccaggtggta tcattctgac cgggggcgca gcggcattac cgggcgtcgt cgatctggcg
      601 aaagaaatct tcgaaatcaa cgtcaagctg tatatcccag aacagatggg gatgcgcaat
      661 ccgatctatg caacgagcat cggcctgatc aagtatgtag ctgggttgga tgatatttac
      721 cgtgtcgcca aaggaaaagt acaggcgaca ggaaaagtga tccctttgca gtccaaaact
      781 gtgaaacaac cggcagtcat agaagaacct gtctatgaaa cggagactta tgccgatgag
      841 gaaaattacg aagaaagttt agtcggaaaa ttaaaacgtt ggttcaacaa tttatttgaa
      901 taatcacgct gtatataaaa tgaatggtat gaaataggag gaaatgaaat ggaattggaa
      961 ttcgattcaa gcatgaatga tggagcaaaa attaaagtta tcggtgtcgg tggggcgggt
     1021 agcaatgccg taaaccgtat gatcgaagaa ggcgtacaag gtgtggaatt catcgtcgcc
     1081 aacacagata cgcaagcctt aaatgcatca agagcggaaa caagaatcca actcggacct
     1141 aaattgacga aagggttggg tgcaggctcg attcctgaag taggccgcaa agctgctgaa
     1201 gagagcgaac aacaaatttc tgaagccttg gctggagcag atatgatctt tgtcacatgc
     1261 ggaatgggtg gcggtaccgg tacgggtgca gctcctatcg tagccagaat cgcgaaggac
     1321 ctgggcgcac tgactgtcgg tgttgtaacg cgtccattca cttttgaagg cccgaaaaga
     1381 ggccgttatg cagccgaagg aatcgctgaa ttaaaagcga acgttgatac attggtcatc
     1441 atctcaaaca atcgcctatt ggaaatcgtg gacaaaaaga ctcctatgct ggaagccttc
     1501 cgtgaagcag ataatgtctt gcgccaaggg gttcaaggga tctctgacct gatcacagct
     1561 ccaggatacg tcaacttgga cttcgctgat gtgaaaacag tcatgaaaga tcaaggttct
     1621 gccttgatgg gtatcggtat cgcctccggc gaaaaccgta cggctgaagc aaccaaaaaa
     1681 gccatttctt caccattgtt ggaagtttcc atcgatggcg ctgaacagat cctgttgaac
     1741 atcacgggtg gttcggactt gacgttgttc gaagcgcaag acgcgagtga tatcgtagca
     1801 caagcttcaa catctgaagt caatatcatc ttcggtacat ccatcaacga aaacttaggc
     1861 gatgaagtta ttgttaccgt aatcgctaca ggaatcgacg acaaaaaaaa agatgaaaaa
     1921 aaaaccgcta atcgcggagg aagtgctttt aagggacgta aagaagtagg gaacacacct
     1981 gtttcatacc cagaaaaaca actcccgact gaaagagcag aagaaaaacc agccaaagat
     2041 ctattcggcg attgggatat ccgtagagac acaagcgtga gggaacaggc gccaaaatcg
     2101 gcccaacctg agccagaaga atactctgtg aaacaaacag aagataatta tccacgttat
     2161 cacgaaaata aagaagacag aaatggtgga gaggattcat tggacacacc acctttcttc
     2221 cgcaagagac gcagataagt atgactattg aacaaaattg ccggaaagtt gagtcaactg
     2281 tggaagcagc ctgccttaca gccggacgca aacgcgagga agtaacggtt gtcgccgtca
     2341 cgaagacaaa acctgtagag caagtggaag aactatatcg cttgggctac cgtcattttg
     2401 cagaaaatcg ccctgaaggt ctgatcgaaa agcagacagc tcttccacaa gaagacatct
     2461 actggcatta tatcggtact ttacagacaa ggaaagtgaa gcaggtgatc aatcgtatcg
     2521 cctacttcca cgcattggat cgcatgtcat tggcaaagga aatcaatgaa cgggcggaaa
     2581 agcctgtttc ttgcttcgtg caggtcaacg ttacagggga aagctcgaaa catgggcttt
     2641 ccgcatccga ggcaatacct ttcatcaaga gtctggaggg cttcccaaat atcactgttg
     2701 tcggcttgat gacgatggca cccattgatg caacagacgt ggagttgcgc cgatgtttca
     2761 aagatctaaa aataatacaa gagcaagtag cggcgttgaa cttggctcac gccccctgta
     2821 cggaaacgag catgggcatg agccaggatt at
//
LOCUS       DAOMUJ010000222         2848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10059_length_2848_cov_2.066237, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000222 DAOMUJ010000000
VERSION     DAOMUJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10059_length_2848_cov_2.066237"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1507)
                     /locus_tag="PLT73_06175"
     CDS             complement(191..1507)
                     /locus_tag="PLT73_06175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM20000.1"
                     /translation="MKTIGKNRHLFFLMLVLLFVGLGSFGTVALANYTSVSISASIVN
                     VRSGPGLSYDIMTQIQGGSSVNVLADKNEWYKVRLSDGRIGWVASWLINNTEIAATSE
                     LLATVLVPSANIRQENNETSEVVGTAIAGEKFALLYEENGWSQIQYNNKVAWILSELI
                     EISPGSIQLAPETVDTTAVVADDTNPKVIITMGGVNVRSSASTGSDILFIAEKGEEYE
                     LISESGDFYKILYEGNKEGYVASWLVEKSESLGAPVTASSTTTLAEATIVIDPGHGGE
                     DPGAEGTYIYEKEVTLKTAQAVAEKLRSAGANVILTRTSDIYVTLEDRAEMSNVNNAD
                     LFISLHYDSTASGTSATGFTTYYYADEDIPLANLVDNHLADTLPLQDNGSKFGDFLVT
                     RENDQPALLLELGYMNNESDVKTFNSDYYRSLVAESIYQALTEYFQ"
     gene            2000..>2848
                     /gene="hisS"
                     /locus_tag="PLT73_06180"
     CDS             2000..>2848
                     /gene="hisS"
                     /locus_tag="PLT73_06180"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRM20001.1"
                     /translation="MIQKPKGTADIFLQEAEIWQYVEETARILLHDYQFSEIRTPMFE
                     SYELFSRGVGDTSDIVSKEMYDFFDKGERHIALKPEGTAPVVRAYVENKLFGPEHPKP
                     LKVYYISPMFRYERPQGGRMRQFHQLGVEVFGSVNPATDVETMALAWDILKELELSDL
                     RLVINSLGKTEDRIRYREALIAYLEPFHDELSKDSQTRLHKNPLRVLDSKDKRDKEIV
                     AKAPSILEFLSDDSRKHFEAVQEMLQALEIPFTINSNMVRGLDYYQDTIFEIMTSSAV
                     FGAETTI"
BASE COUNT          782 a          621 c          673 g          772 t
ORIGIN      
        1 atttgcatgg gagtcttagg gattcatccc ttagactccc agtacaaggt gaccgtaggg
       61 aacgttgcgg agcacgattt cctaaattgt catgactctt gcgatttcaa tcgcttagag
      121 gcatgataca cttgaccgat taggtcacag tgtttcctta ctcagggctg aacgaacccg
      181 ttccgctttc ttattggaaa tattcggtca atgcttggta gatgctttct gccaccagcg
      241 agcgatagta gtcggaattg aaggtcttga cgtcggattc gttgttcata tagccaagtt
      301 ccaacagcaa ggccggctga tcattttcgc gtgtaaccag gaaatctccg aacttggatc
      361 cgttgtcctg caacggcaag gtgtcagcca ggtgattatc gaccagattg gctaacggga
      421 tatcctcatc tgcgtaataa taagtcgtaa agccagtcgc tgaagtaccg cttgcggtgg
      481 agtcgtaatg cagactgatg aataaatcag cattgttgac attactcatt tctgcgcgat
      541 cttccaacgt gacatagatg tccgaggtgc gcgtcaatat gacgttggct ccggcactgc
      601 gtaatttttc cgccacagct tgagcggttt tgagtgtgac ttccttctca taaatataag
      661 taccttccgc acccggatct tctcctccgt ggcccggatc aatcacgatt gtcgcttctg
      721 caagcgtggt ggtcgatgac gcagtgactg gcgcaccgag tgattcggat ttttccacca
      781 accaggaggc gacgtaacct tccttgtttc cctcatatag gatcttatag aaatcaccgg
      841 attcgctgat cagttcgtac tcttcgcctt tttcggcaat gaacaggata tcgctgcctg
      901 tcgaagcact gctgcggaca tttacgccac ccatcgtgat gatcactttc ggattagtgt
      961 cgtctgcaac aacggcagtg gtatcgactg tttctggcgc caactgaatg ctgcctggtg
     1021 aaatttcgat cagctcagac aaaatccaag caactttatt gttgtattgg atttggctcc
     1081 aaccgttttc ttcgtataat agggcgaatt tttcacctgc aatagccgtt ccgacgactt
     1141 cagatgtttc attgttctcc tgccggatat tggcggaggg tacaaggacc gttgccagca
     1201 attcggaggt tgccgcgatt tcggtattgt tgatcagcca gcttgctacc caaccgattc
     1261 ttccatccga taaccggact ttataccatt cgttcttatc cgctaaaacg ttgacagagg
     1321 atccgccttg tatttgcgtc ataatatcgt aagataagcc cggaccggac cggacgttta
     1381 cgatgctggc ggagatgctg accgaagtat agttggctaa tgctaccgta ccgaaggagc
     1441 ccaatccaac aaatagcaaa acaagcatca agaagaacag atgtctattt tttccgatag
     1501 ttttcactga ttgtctcctt tctggtttgg gatgcataaa atttacaaaa attgttcaat
     1561 tcttctatca ttatagcaaa gatacgttat ccaatgcaaa gccttttcag aaattataaa
     1621 gttgtgctgt atctggacag gggagaagta catcaagatg aaaatatgat gaaaacagta
     1681 ttgacaattc gaagcttatc ttgtatcgtt aaggaaatta taaatgtcca gtgaagatcg
     1741 aagtagaata gattggtttg attcagagag gattgccgcg gctgagagca ctcccaaacg
     1801 tttatttgaa agacaggttg gaggattaat agtgaaaagc tattacgcca cgaacgttac
     1861 tcgttaaagg aaagctatgc tttcgaataa aggtggcacc acgtctgact aatcgtcctt
     1921 ttccatgatg gaaagagggc tatttttttg ctttttttca gacaaaaaac agtatgaagc
     1981 gaaaggaatg gttataaaaa tgatacaaaa accaaaaggg actgctgaca tatttttgca
     2041 ggaagcagaa atttggcaat atgtggaaga gacagcccga atcttattgc atgactatca
     2101 attttcggaa atccgcacgc cgatgtttga aagctatgag ttgttttcca gaggcgttgg
     2161 cgatacgagc gatatcgtat cgaaagaaat gtatgatttc ttcgacaaag gcgaacgcca
     2221 tatcgcattg aagccagaag gaacagcccc ggtggttagg gcttatgttg agaacaaact
     2281 gttcggtcct gaacatccaa aaccgctgaa agtctattac atcagcccga tgtttcgcta
     2341 cgaaagacca caaggcggaa gaatgcgaca attccaccaa ttaggtgtgg aagttttcgg
     2401 aagcgtgaat ccggcgaccg atgtcgaaac aatggcattg gcatgggata tactgaagga
     2461 attggaattg agcgatctga gattggtcat caattctttg gggaaaactg aagaccggat
     2521 cagataccgc gaagcgctga tcgcctacct agaaccgttc catgatgaac tgagcaaaga
     2581 ttcccagact cgtttacata agaatccgtt gcgcgtgttg gacagcaaag acaagcgcga
     2641 caaggaaatc gttgccaagg caccaagcat cttggagttc ttgtcggatg attcaaggaa
     2701 acattttgag gcggttcagg aaatgcttca ggcattggaa attccgttca ctatcaacag
     2761 caatatggtc cgcggactcg actattacca agacactatc tttgaaatta tgacttcgtc
     2821 cgcagtcttc ggagccgaaa cgaccatc
//
LOCUS       DAOMUJ010000223         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10159_length_2831_cov_2.437320, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000223 DAOMUJ010000000
VERSION     DAOMUJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_2831_cov_2.437320"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLT73_06185"
     CDS             <1..1116
                     /locus_tag="PLT73_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20002.1"
                     /translation="IRAFGNEPFEENRFNDANEDYTYTNRFVQRLMSLLMPAMMLVMN
                     GVSLLIIWFGSQQVADSALQIGDMMAYIQYVMQIIMAFLMISMMFIFLPRASVSATRI
                     GEVLDTDLSITDPNDAQPSLKNEGLITFKDVSYHYPKASENVLNNISFTARPGETTAI
                     IGSTGSGKSTLINLIPRFYDVTDGAIEIDGIDIRKRTQADLRANIGYVPQKGMLFSGN
                     IVSNIAYGKDDASTDEIMKALEVAQAKNFVSEMEDGIETAIAQGGGNVSGGQKQRLSI
                     ARALVKKAPIYIFDDSFSALDFKTDAALRSALDAYTDNATVLIVAQRISTIMDAEQII
                     VLDKGRIVGIGTHDALMKNCETYQEIAKSQLSEEELA"
     gene            1113..>2831
                     /locus_tag="PLT73_06190"
     CDS             1113..>2831
                     /locus_tag="PLT73_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20003.1"
                     /translation="MSEMKQRRTQGGPGGGHGPMGGMSAPVVKAKDFKGGFKKLAAYL
                     SPFKWLMAVVFILAISSSLFGIVGPKIMALAIDKLVAGIMLKFTGGSGEIDFDYIGTV
                     LLGLLGLYVLSATFSYLQGFLMSGVSAKVSYKLRKDIMEKINKLPLAYFHKNSQGDVL
                     SRITNDVDTLNTSLNQSLTQIITSITTLVGVFIMMLTISWSLTLVILGVLIVSMMLLM
                     LIMSKSQKHFANQQKYLGAVNGHVEEMYGGHVVMKAFNGEAKSVAAFDKENDQLIEAG
                     FKAEFLSGLMMPLLSLIGNLGYVVVCIMGGAMAAAGNMTIGDIQAFLQYVRNFTQPLT
                     QVAQVGSQLQRMVAASERIFEFLEEEEETVTEAKIEMATADVKGNVRFEHVKFGYEPD
                     HLVIKDFSADIKEGQKVALVGPTGAGKTTMVKLLMRFYDLTGGAIYIDNHKLTDFTRS
                     DLRTEFGMVLQDTWLYNGTIMENIRYGKLDATDEEVIAAAKAAQVDHFVRTLPDGYDM
                     VLNEEANNVSQGEKQLLTIARAILADPQIMILDEATSSVDTRTEVLIQKAMDTLMHGR
                     TSFIIAH"
BASE COUNT          737 a          643 c          772 g          679 t
ORIGIN      
        1 atccgcgctt tcgggaatga accatttgaa gagaaccgat tcaacgacgc caatgaagat
       61 tacacctata caaaccgctt tgtgcagcgt ttgatgtcgc tgttgatgcc tgccatgatg
      121 ctcgtcatga acggtgtttc gctgctgatc atctggttcg gaagccagca agtcgctgat
      181 tctgccctgc agatcgggga tatgatggcc tatatccaat atgtgatgca gatcatcatg
      241 gccttcttga tgatttcaat gatgttcatc ttcttgccgc gtgcttctgt atcggcaaca
      301 cggatcgggg aagtgttgga tacggatttg agcatcacgg atccaaacga cgcgcaaccg
      361 agcttaaaga atgaaggcct catcaccttc aaggatgttt cttatcatta tccgaaggcg
      421 agcgagaacg tgttgaacaa catttccttt accgctagac caggcgaaac gacggcaatc
      481 atcggttcga ccggttctgg taaatcgaca ttgatcaatc tgattccgcg cttctatgat
      541 gtgacggacg gcgccatcga aatcgacggc atcgacatcc gcaaaaggac acaagccgat
      601 ctgcgcgcta acattggcta tgtcccgcag aaggggatgc ttttctcagg gaatattgta
      661 tccaacatag cgtatggaaa agatgatgct tcgacggatg aaatcatgaa agcactcgaa
      721 gtcgcccaag cgaaaaactt tgtttctgag atggaagacg gcatcgaaac ggctattgca
      781 caaggcggcg gcaacgtcag cggtgggcag aaacagcgct tgtccattgc gcgtgcattg
      841 gttaaaaaag cgccgatcta tatttttgac gatagtttct cggcgttgga cttcaaaacg
      901 gatgcggctt tacgttcggc tttggatgcc tacacggata acgcaactgt cctgatcgtg
      961 gcgcagcgga tcagcacgat aatggatgcg gaacagatca tcgttctaga taaaggcagg
     1021 atcgttggga tagggactca cgatgcgttg atgaaaaact gtgagactta tcaagaaatt
     1081 gctaaatctc agctttcgga ggaggaatta gcatgagcga aatgaaacaa agaagaacgc
     1141 aaggcggacc gggcggaggc catggtccca tgggtgggat gagcgctccg gtggtgaaag
     1201 cgaaggactt caaaggcgga ttcaaaaaat tggccgcgta tctgtcgccg ttcaaatggc
     1261 tgatggcagt tgtcttcatt ttggcgattt cgtcctctct attcgggatt gtcggaccaa
     1321 aaatcatggc tttggccatc gataaattgg tcgccggcat catgctgaaa ttcactggcg
     1381 gatccggcga aatcgatttc gattacatcg gtaccgtact gttggggctt ttgggcttgt
     1441 acgtgctgag tgccactttc tcttatctgc aagggtttct gatgtcgggt gtctccgcaa
     1501 aagtttccta taaactgcgc aaagacatca tggagaaaat caacaaattg ccgctggctt
     1561 atttccacaa gaacagccaa ggggacgtgc tttccaggat cacgaatgat gtggatacgc
     1621 tgaataccag tctgaaccag agcctcaccc aaatcatcac gtcgatcaca acactggtag
     1681 gtgttttcat catgatgctg acgatcagtt ggtcgctgac acttgtcatc cttggcgtgc
     1741 tgattgtgtc catgatgctc ctgatgctga tcatgtcgaa gtcgcagaag cacttcgcta
     1801 atcagcaaaa atacttgggt gcggtcaatg gccatgttga agagatgtat ggcggacatg
     1861 tggttatgaa agccttcaac ggggaagcaa aatcggttgc cgctttcgat aaagagaatg
     1921 atcaattgat cgaagccgga ttcaaagctg aattcctttc cggtttgatg atgcccttgt
     1981 tgagcctgat cggaaatctt gggtatgtcg tagtctgcat catgggtggt gcgatggcgg
     2041 ctgccggaaa tatgacgatc ggggatatcc aagccttcct acagtacgtg cgcaacttca
     2101 cccaaccttt gacgcaagtc gcgcaagtgg ggagtcaatt gcaacggatg gtggctgcct
     2161 ctgaacgaat cttcgaattc ctggaagagg aagaagagac agtgaccgaa gcaaaaatcg
     2221 aaatggcgac cgctgatgtg aaagggaatg tccgtttcga gcatgtcaag ttcggttatg
     2281 aaccggatca tctggtcatc aaggacttct ccgctgatat caaagaagga caaaaagtcg
     2341 cgctcgttgg tcctaccggt gccggcaaga cgacgatggt gaaattgctg atgcgtttct
     2401 acgatttgac gggtggagcc atctacatcg acaaccacaa acttaccgat ttcacgcgca
     2461 gcgatctgcg gacggaattc gggatggtgc tgcaggatac atggctatac aacggcacca
     2521 ttatggaaaa catccgctac gggaaactag atgcgacgga tgaggaagtc attgctgccg
     2581 cgaaagctgc gcaagtggat cactttgtcc ggacattgcc ggatggttat gacatggtcc
     2641 tgaacgagga agccaacaac gtttctcagg gagagaagca attgttgacg atcgcgcgcg
     2701 ccatccttgc cgatccgcag atcatgatcc tggatgaagc gacgagttcg gtcgatacgc
     2761 ggacggaagt gctgatccag aaagcgatgg acacgctgat gcacggcagg acgagcttca
     2821 tcatcgcgca t
//
LOCUS       DAOMUJ010000224         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10160_length_2831_cov_2.277017, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000224 DAOMUJ010000000
VERSION     DAOMUJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10160_length_2831_cov_2.277017"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..430)
                     /locus_tag="PLT73_06195"
     CDS             complement(134..430)
                     /locus_tag="PLT73_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRM20004.1"
                     /translation="METFNLGDGLIVTIVAMIVVFAVLASLWFLVELVHKLVGEPAPA
                     TTAPIDREEASQPVLLDPGVDEQRAKAAAIMALVAAYEAHPAKKFEIVDVTRIK"
     gene            complement(450..1892)
                     /locus_tag="PLT73_06200"
     CDS             complement(450..1892)
                     /locus_tag="PLT73_06200"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HRM20005.1"
                     /translation="MEKEKKVLFTETVLRDAHQSLMATRMSTDDMLPIVEKMDEAGYY
                     ALECWGGATYDAAIRFLHEDPWERLRQIRKRAPHAKLQMLLRGQNLIGYRHYADDIVD
                     RFVGKAVENGIDIFRIFDALNDTRNLKASLEAVKKYGAHAQLTICYTISDVHTIPFYT
                     DLAKELTEMGADSICIKDMAGILTPKVAKELIPAIKAVIDVPLNIHTHATSGVSQMTY
                     MAAIEAGADIIDCAISPFAEGTSQPPTESMALVLEELGYSTGLDIAKLEEIADYFKPI
                     RDKYIASGVLDPKMLSVDPKALIYQVPGGMLSNLNSQLKQANASDKYEAVLREVPRVR
                     ADLGFPPLVTPMSQMVGTQAVFNVLTGERYKMVPNEVKDYLHGKYGKTPVPVSDAFRR
                     SIIGDDAVITDRPADHLEPEFERLKAELGDLARTDEDVLTYALFPQVGKAYLQEVYYP
                     TIPEEQAETKAPSPGETTETVVRIQARYRS"
     gene            complement(2113..2487)
                     /gene="dhaM"
                     /locus_tag="PLT73_06205"
     CDS             complement(2113..2487)
                     /gene="dhaM"
                     /locus_tag="PLT73_06205"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HRM20006.1"
                     /translation="MSEQYGILLVSHVAELADGLTRLLKQVAKDVTVKAVGGAEGGGI
                     GTSFDKVIAVLNEMEEPEIFAFYDLGSAKMNLELVSEMTEKNLIICDAAFVEGAYLTA
                     ALLQADTPHEKICEQLQELYVK"
     gene            complement(2480..>2831)
                     /locus_tag="PLT73_06210"
     CDS             complement(2480..>2831)
                     /locus_tag="PLT73_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM20007.1"
                     /translation="GLLYGQIFLHMSEAFQEDNDLMDSLTSGLEGIQTFGPTELKEKT
                     LVDVWIPVMEDIRNKRLTLEKINEYVTSTKDFQAKKGRAVYVSEQLNGHIDSGAASCG
                     YFFEAMLEAGVYDE"
BASE COUNT          672 a          783 c          601 g          775 t
ORIGIN      
        1 gtctgcctta ggttgctccg tcatgactgc atcatccggc aactccctca ctttgacatg
       61 ataaacttgc ccgtcaattt cgatctcata atttttcata aaaaatcctc cctttatagt
      121 cagcagatgg catttatttg atacgcgtta catcaacgat ctcgaacttc ttagccggat
      181 gagcctcata tgcggcgacc aatgccatta tcgccgcggc tttggcccgt tgctcatcca
      241 cacccggatc cagcagaacg ggctgactag cttcttcgcg atcaatcggt gctgtagttg
      301 ccggtgctgg ttcacctacc agcttatgca ccagttcgac caagaaccaa agacttgcca
      361 aaacagcaaa cacgacgatc atcgcaacaa tcgtgacaat cagaccatct cctaaattga
      421 atgtttccat ttcccttcac ctcttcctct tagctgcgat agcgggcttg gatccgaacc
      481 actgtttccg tcgtctcgcc tggagatggc gcttttgttt ctgcttgttc ttctggaatg
      541 gtcggatagt agacttcctg cagatacgcc tttccgacct gagggaacaa agcataagtc
      601 aatacgtcct cgtccgtacg cgccaagtca cccaactccg ccttcaatct ttcgaactcc
      661 ggctccagat ggtctgccgg gcgatccgtg atgacggcat catccccgat aatgctgcgt
      721 ctgaacgcgt cgctcaccgg gaccggtgtc tttccgtatt ttccgtgcaa gtaatccttc
      781 acttcatttg ggaccatttt gtaacgttct cccgtcagaa cattgaaaac agcctgcgtc
      841 ccgaccattt ggctcattgg ggtgaccaac ggcggaaagc ccaagtccgc tctgacgcgg
      901 ggcacttccc gcagcacagc ctcgtactta tcgctggcat ttgcctgttt gagttgcgaa
      961 ttcaagttcg acagcattcc gcccggtact tgatagatga gcgctttggg atcgactgac
     1021 agcatctttg ggtccagcac acccgaagcg atgtacttgt cgcgaatcgg tttgaaataa
     1081 tcagcaatct cttccaattt ggcaatgtcc aagcccgtac tgtatccgag ttcttcaagc
     1141 actagcgcca tcgattcggt aggcggttga cttgttcctt ccgcaaaagg cgaaatggca
     1201 cagtcaatga tgtccgcacc cgcttcgatg gcagccatgt aggtcatctg tgaaacgccg
     1261 ctggtggcat gcgtgtggat attcagcggc acatcgataa cggctttgat ggccggaatc
     1321 aactctttgg ctactttcgg cgtcaatata ccagccatat ccttgatgca gattgagtcc
     1381 gcacccattt cagtcaattc cttggccaaa tcggtgtaaa atgggatcgt gtggacatcg
     1441 ctgatggtgt aacaaatcgt cagttgcgca tgggcgccgt attttttgac ggcttcaagt
     1501 gatgccttca agtttctcgt gtcattcaag gcatcaaaaa tccgaaaaat atcgatgcca
     1561 ttctcgacag ctttgcctac aaaacgatcc acgatatcat ctgcataatg gcggtagcca
     1621 atcaagttct gtcccctaag cagcatctgc agctttgcat gcggagcacg cttgcggatt
     1681 tgccgcagtc tttcccacgg atcttcatgc agaaaacgga tagccgcatc ataagtggcc
     1741 ccgccccagc actcgagcgc gtaatacccg gcttcatcca ttttctcaac aatcgggagc
     1801 atgtcgtccg tagacatacg tgtcgccatc aagctttgat gcgcatcacg cagaacggtc
     1861 tcagtaaaaa gcactttttt ttctttttcc aatccgaaca catccattct tttctttagc
     1921 gctaaaattg taagcgtttt ctgtagtttt agcttacact aaattgaccg atatttcaat
     1981 ctgtagataa aaaatacgag aaagaatata gaaacaatac accttcaata gggggaaacc
     2041 gtaaataaaa aagactgcac cttggcagtc tttttcttaa ttgtgtttgt cactacaatt
     2101 ttgctcagtg tttcatttta cgtacaattc ctgcaactgc tcacagattt tttcatgggg
     2161 cgtatccgcc tgcagcaaag ctgctgtcag ataggcgccc tccacaaatg ccgcatcgca
     2221 aatgattagg tttttttcgg tcatttcact taccaactca agattcatct tggcgctgcc
     2281 cagatcataa aaagcaaata tttcaggctc ctccatttca ttgagcactg ctatgacttt
     2341 atcaaaactc gtgccgatgc cgccgccttc cgctccgccg accgctttca cggtgacgtc
     2401 ctttgctacc tgtttcagca atctggtcag cccgtcagcg agctccgcaa catgtgagac
     2461 caataagatg ccgtactgtt cactcatcgt acacacccgc ttccagcatg gcctcaaaga
     2521 aataaccgca ggaggctgca cctgaatcga tatggccatt cagttgttcc gacacataga
     2581 ccgcgcgtcc ctttttcgcc tggaaatcct tcgtgcttgt gacatattca ttgatttttt
     2641 ccaacgtcag acgcttattc cggatgtctt ccatgaccgg aatccaaaca tcaaccaagg
     2701 tcttttcctt cagttccgta gggccgaacg tttggatgcc ttcaagcccg cttgtcaggc
     2761 tgtccatcag atcgttatct tcttgaaaag cctccgacat atgcagaaag atttgcccat
     2821 aaaggagtcc g
//
LOCUS       DAOMUJ010000225         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10203_length_2822_cov_1.493675, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000225 DAOMUJ010000000
VERSION     DAOMUJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_2822_cov_1.493675"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLT73_06215"
     CDS             <1..481
                     /locus_tag="PLT73_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM20008.1"
                     /translation="LVLTMFVRTLAVPVVHMMDAAAEQGDYRKIELLQIGAIAMQGLR
                     IAIPAALLLFIPADAVRSGLEAMPGWLTDGMAIGGGMVVAVGYAMVINMMATKEVWPF
                     FIIGFVVAAVSELTLIALGALGVAMALIYLNLSKMGGSGNGGSSNTGDPLDSILNDY"
     gene            499..1425
                     /locus_tag="PLT73_06220"
     CDS             499..1425
                     /locus_tag="PLT73_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM20009.1"
                     /translation="MAQNTVDKKFVLTKKDRLAVAWRSTFIQGSWNYERMQNGGWAFA
                     MIPAIKKLYPAKEDRVLALKRHLEFFNTHPYVASPVLGVTLALEEERANGAPVDDVTI
                     QGVKVGMMGPLAGVGDPVFWFTVRPILGALGASLALTGNILGPILFFVAWNAIRWGFM
                     WYTQEFGYKAGSKITEDLSGGILQDLTKGASILGMFVLAALVQRWVSISFAPTVSSVK
                     LQEGAFIDWENLPSGTAGIQEALMQYTSGLSLTDTKVTTLQNNLDQLIPGLAALLLTF
                     LCMWLLKKKVSPIVIILGLFVVGIGGRLIGLL"
     gene            1553..1933
                     /locus_tag="PLT73_06225"
     CDS             1553..1933
                     /locus_tag="PLT73_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HRM20010.1"
                     /translation="MVESLNAKVDMVVDGTSYMGLTDYGKIMIGDQAFEFYHHRDPRK
                     FIQIPWEEVDYVLASVMFKGKWIPRFALQTKKDGTYTFSAKNPKDVLRAIRIYVSPDH
                     MVQSLGFFDVLKNAFKHMFNKKAS"
     gene            2002..2499
                     /locus_tag="PLT73_06230"
     CDS             2002..2499
                     /locus_tag="PLT73_06230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HRM20011.1"
                     /translation="MVMDIRLVRIDDRLIHGQVATVWTRLSKAERIIVISDEVARDHL
                     RKTLLRQVAPPGVKVNVIPVEKFVEIYFDDAHNQFRAMLLFTNPLDVARIVQRGVQLD
                     SVNIGSMSYSDGKKMITNAVAVDKRDVQAFEYLNFKGIHLDIRKVVADNKLDLMQVLK
                     KEGLV"
     gene            2592..>2822
                     /locus_tag="PLT73_06235"
     CDS             2592..>2822
                     /locus_tag="PLT73_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRM20012.1"
                     /translation="MLKEACAENFTNVPELIERGAKRIELCDNLAVGGTTPSVGVIKI
                     TAEYCSDKDASIIVILRPRGGDFVYSLMEKAIM"
BASE COUNT          760 a          554 c          731 g          777 t
ORIGIN      
        1 attggttttg acgatgttcg ttcgtacatt ggcagttcct gttgttcata tgatggacgc
       61 agctgcagaa caaggtgact atcgtaaaat tgagttgctt caaattggag caattgccat
      121 gcaaggtttg cggattgcaa ttcctgcagc attgcttttg ttcatccctg cagacgcagt
      181 ccgttctgga ttggaagcaa tgccgggctg gctgactgat ggaatggcaa tcggtggtgg
      241 tatggttgta gcggttggtt atgcaatggt aatcaatatg atggccacca aagaagtatg
      301 gccgttcttc atcatcggtt tcgtcgttgc cgctgtatct gaattgacat tgatcgcatt
      361 aggagcccta ggtgtcgcaa tggcattgat ctacttgaac ctttctaaaa tgggcggttc
      421 cggtaatggc ggttccagca atactggcga tccacttgat tcgatactca atgactatta
      481 ggaaaggaga ctgcaaacat ggcacaaaat acggtagata aaaaattcgt attaactaaa
      541 aaagatagac tagctgtcgc ttggcgttcc actttcattc aaggatcatg gaactacgaa
      601 cggatgcaaa atggtggctg ggctttcgca atgattccag caatcaagaa actttatcca
      661 gcaaaagaag atcgcgtcct agcgttgaaa cgccacttgg aattcttcaa cacacatcca
      721 tatgtggcat ctccggtatt aggagtaacg ctggctctgg aagaagaacg tgcaaacgga
      781 gctccagtag atgatgttac gattcaaggt gtgaaggtcg gaatgatggg acctcttgcc
      841 ggtgttggtg accctgtgtt ctggtttaca gtccgtccga ttttgggagc tttaggcgca
      901 tccttggcct taacaggaaa catcctagga cctatcctgt tctttgttgc ctggaacgct
      961 atccgttggg gattcatgtg gtatacccaa gaattcggtt ataaggctgg atcaaaaatc
     1021 actgaggatt tatctggcgg aatcctgcaa gatttgacaa aaggagcttc cattctgggg
     1081 atgttcgtat tggcggcctt ggtgcaacgt tgggtatcga tctcgtttgc tccaaccgtt
     1141 tcatcagtca agcttcaaga aggcgcattt atcgattggg aaaacctgcc aagtggcact
     1201 gccggcatcc aggaagccct gatgcagtat acttcaggct tgtcgttgac ggacactaaa
     1261 gtcacaacct tgcaaaacaa cctggatcaa ttgatccctg gattggcagc gctgctgttg
     1321 acattccttt gcatgtggtt gttgaagaag aaagtatctc caatcgtgat catccttggc
     1381 ctgttcgttg ttggtatcgg cggacgcttg atcggattgc tgtaattttc ggatgcatca
     1441 aaaataacga ttatgaggct caggcaaacc agcctgagcc ttgtttttct tgtaaaaagt
     1501 aaagagtgta tattatactc aaatcagaca caaagagaga ggggcatata caatggttga
     1561 atccctgaat gcaaaggttg acatggtagt ggatggtact tcatatatgg ggctgaccga
     1621 ctatggtaaa attatgattg gagatcaagc gtttgaattt tatcatcatc gtgatccgcg
     1681 aaagttcatt caaattcctt gggaagaggt tgattatgta ctggcatccg tgatgttcaa
     1741 agggaagtgg attccacgtt ttgcgcttca aacaaagaag gatggtacgt acactttttc
     1801 agcaaaaaat cccaaggacg tgttgcgtgc gatccgcatc tatgtaagcc cggatcatat
     1861 ggttcaatca ttgggcttct tcgatgtttt gaagaatgcc ttcaagcata tgttcaataa
     1921 gaaagcatcc taaatagtgg caggaaatca atttgcatac ggtttacggt tgctggatga
     1981 acgatttaag gaggttctaa tgtggtaatg gatattcggt tggtacgaat cgacgatcgt
     2041 ttgattcacg gacaggtggc aaccgtgtgg acaagactaa gtaaggcaga gcggattatt
     2101 gtcatcagcg acgaggtggc gagggatcat ctgcgtaaga cgctcttgcg gcaagtcgct
     2161 ccacctggtg taaaggtcaa tgtgattcca gtggagaagt ttgtcgaaat ttacttcgat
     2221 gatgcgcata accaatttcg ggcgatgctc ttgtttacga atccactcga tgtcgcacgc
     2281 atcgtccaaa ggggcgttca gcttgattcc gtcaatatcg gcagtatgag ttattcagat
     2341 ggaaaaaaga tgatcacgaa cgcagtagcg gttgataaac gcgatgttca ggcattcgag
     2401 tacctgaatt ttaagggtat ccacttggat atccggaagg tcgtcgctga caataaattg
     2461 gatctcatgc aagtgctgaa aaaagaaggt ttggtctgag cgcatattaa aagaagacac
     2521 gaagcgaatg gaacagcgcc ttgtgccttc tttgttttta cggaatggta tattatccag
     2581 ggggtgacag cgtgctaaaa gaagcatgtg cagaaaattt tacgaacgtc ccagaactca
     2641 tcgagcgcgg ggctaaacgg attgaattgt gcgataattt ggcagtgggg ggaacaacac
     2701 caagcgttgg cgtcatcaaa attacagcgg agtattgctc cgacaaagat gcgtccatca
     2761 tcgtgatact gcgaccacga ggcggagatt tcgtttattc tcttatggaa aaagccatca
     2821 tg
//
LOCUS       DAOMUJ010000226         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10227_length_2816_cov_2.266208, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000226 DAOMUJ010000000
VERSION     DAOMUJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10227_length_2816_cov_2.266208"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..681
                     /locus_tag="PLT73_06240"
     CDS             226..681
                     /locus_tag="PLT73_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM20013.1"
                     /translation="MKIDFLKKEIWDLMRTIQVSKDMVMCPVAKDYPITPLQLRILME
                     VNVSENLSLNRLAKVMDMNNGNVSTLCKKLEQQGYLTRERRADDERFITLKLTHNGQA
                     ILQKMEQDIEGKYCLLMEGVSEERLEKIAVGIKELQLLIQEMNEQQRED"
     gene            692..2566
                     /gene="ftsH"
                     /locus_tag="PLT73_06245"
     CDS             692..2566
                     /gene="ftsH"
                     /locus_tag="PLT73_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM20014.1"
                     /translation="MADDKNKNNRKWNVNLNNENPDNRKRTAYYLLMALSVLMFLNYF
                     ALPSYLNRSVEEVTYSTFLNQVSTGDVSEVKLEENQITFVATDDAGDKQVYITGLIDD
                     PTLVTRLEEADVTFSKDIPTEASPILSILVSWVLPLLLFWGLGSMLGKQMAKRMGGGA
                     GGALSFGKSNAKVYVKADDAKTFKDVAGQDEAKEALSEVVDYLHQPKKYQDIGAKNPK
                     GILLVGPPGTGKTLLARAVAGEADVPFFSISGSEFVEMFVGRGAAKVRDLFKEANEKA
                     PCIVFIDEIDTIGKKRDNGNFGGGNDEREQTLNQLLAEMDGFEANKGIILLAATNRPE
                     SLDAALLRPGRFDRRVPVELPDLAGREAILKVHAKNYKMDPTIDYNTIARATSGASGA
                     ELANIINEGGLRAVREGRSAVTQSDLEESVETVIAGYQRKNAVISPKEKEIVSYHEIG
                     HALVAAKQTDSAPVHKITIIPRTSGALGYTMQIEEGEKSLMTKQELFNKIVTLAGGRA
                     AEEIVFGSITTGASNDIEQMTKMARAMITRYGMSDTFDMMQIETQTNKYLGGDTSMNV
                     SAGMAEQVDREVLDIIKLAHEKAIAILNENREKLHELSHHLLIEETITGAEFMKILEA
                     "
BASE COUNT          787 a          682 c          734 g          613 t
ORIGIN      
        1 gtcccttgca accggcgcac gccgcaaaca tatcttatgg aatactttca agcatcttca
       61 cgatttcttc acactcacgc tttatagtag gctcacagca ttcctagcga gagggatctc
      121 ttaggaattg cttaagacta agttgaaccg cttgcacgaa aaatagtttt gatttaaaat
      181 agttttatat cacaactatt atccgataaa ggagcggatt tcaatatgaa aattgatttt
      241 ttgaaaaaag aaatctggga tttgatgcgg accatccaag tttccaaaga tatggtcatg
      301 tgcccggtcg ctaaagacta tcccatcacc ccgctgcaat tgcggatttt gatggaagtg
      361 aatgtgagtg aaaatctatc actcaaccgt ttggcgaagg tgatggatat gaacaacggc
      421 aacgtctcca ccctctgcaa aaaattggaa cagcaaggct acctgacgcg ggaacggcgc
      481 gcggatgatg aacgtttcat cacgctgaaa ctcacacaca atggccaagc gatccttcag
      541 aaaatggagc aggatatcga aggcaaatac tgcttgctga tggaaggcgt gtccgaggaa
      601 cggcttgaaa aaatcgcagt cggcatcaag gaactgcaac ttttgataca agaaatgaac
      661 gaacaacaaa gagaggattg actcaacatt tatggcagat gataaaaaca aaaacaaccg
      721 caagtggaat gtgaacctga acaacgaaaa ccccgacaat cgcaaaagga cagcctatta
      781 cttgttgatg gcattgagcg tcctgatgtt cctgaactat ttcgcattac ctagctacct
      841 gaaccgaagc gtggaggaag tgacctacag cacgttcctg aatcaagtgt cgacgggcga
      901 tgtatccgaa gtgaaattgg aagagaatca aatcactttc gttgctactg atgatgcggg
      961 cgacaaacaa gtttacatta ctggtctgat cgatgatccg acactcgtca cccgcttgga
     1021 agaagccgat gtgaccttca gcaaggacat tccgactgaa gcctctccga ttctttcgat
     1081 cttagtttct tgggtcttac cgctcctgct gttctgggga ttgggcagca tgcttgggaa
     1141 acagatggcg aaacggatgg gcggcggtgc cggtggcgca ttgtcgttcg gcaaatcgaa
     1201 tgccaaagtc tacgtgaaag cagacgacgc caagacattc aaggatgtcg ccggacagga
     1261 cgaagccaaa gaagcgctca gcgaagtcgt cgattacttg catcaaccga aaaaatacca
     1321 ggatatcggt gccaagaatc cgaagggcat cctgctggtc ggacctcccg gaaccggtaa
     1381 aaccctgttg gcccgcgctg tcgccggcga agccgatgtg ccgttcttca gtatttccgg
     1441 ctccgaattc gtcgagatgt tcgtcggacg cggcgccgca aaagtccgcg acctgttcaa
     1501 ggaagccaat gaaaaagccc cttgtatcgt cttcatcgat gagatcgaca caatcggtaa
     1561 aaagcgcgac aacggcaatt ttggtggggg gaacgatgag cgtgagcaga ccttgaacca
     1621 attgctggcg gaaatggacg gatttgaagc caacaaaggc atcatcctcc tggccgccac
     1681 caaccgtccg gaatcccttg acgctgcctt actgcgtccg ggccgttttg accgtcgcgt
     1741 accggtcgaa ctgccggact tggcaggacg tgaagccatt ctgaaagtgc atgcgaagaa
     1801 ctacaaaatg gatccaacta ttgattacaa tacgatcgcc cgcgccactt ccggtgcttc
     1861 cggtgccgag ctggcgaata tcatcaatga aggcggcctc cgcgccgtcc gtgaaggccg
     1921 cagcgccgtt acgcaaagcg atctggagga gtctgttgag actgtcattg ctgggtacca
     1981 gcgcaagaat gcggtcattt ctccgaaaga aaaggaaatc gtctcttacc atgaaatcgg
     2041 ccacgcgctg gtagcggcca aacagacgga ttccgcgccg gtccacaaga tcacgatcat
     2101 tccgcgtact tccggtgctt tgggctatac gatgcagatc gaggaaggcg aaaaatcgtt
     2161 gatgacgaag caagaattgt tcaataaaat cgtgacgctg gcaggcggtc gcgccgccga
     2221 agagatcgtc ttcggcagca tcacgaccgg cgcttcgaat gatatcgagc agatgaccaa
     2281 aatggcgcgc gccatgatta cgcgctacgg gatgagcgac acgttcgaca tgatgcagat
     2341 tgaaacccag acgaacaaat atttgggcgg cgacacttcc atgaatgtgt ccgccgggat
     2401 ggccgagcag gtcgaccgtg aagtgctgga catcatcaag ctggctcatg agaaagccat
     2461 tgccatattg aacgaaaaca gagagaaact gcatgaactg tcccaccacc tgctgatcga
     2521 agagaccatt accggtgcgg aattcatgaa gatattggaa gcataacagc tcgcatacga
     2581 ggcacggatt catcagaact gccgatatgt caacaaaaga aagcagtgtg gttgtctcag
     2641 ttcgagatag ccacactgct tatttttgag gcggaaaatg ttcgagatat gctcgcgttg
     2701 tccgattctt tgcggttatc ggacaagcag gactcctaat gcgatgcgga tgtcggatac
     2761 tttgcagtaa tccgacaagc agtgcaccaa atgcggtgag gttgtcggat acattg
//
LOCUS       DAOMUJ010000227         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10237_length_2814_cov_2.500906, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000227 DAOMUJ010000000
VERSION     DAOMUJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10237_length_2814_cov_2.500906"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..805
                     /locus_tag="PLT73_06250"
     CDS             62..805
                     /locus_tag="PLT73_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM20015.1"
                     /translation="MRLGVELGMTLIDTAEMYGDGKSERLVGEAIKGIRDQVYLVSKV
                     YPHRASRNEILAACAESLRRLDTDHLDLYLLHWRGNVPLSQTIAGMEQLKAEGKIGRW
                     GVSNFDIHDMQDLLSMEHGNQCQVNQVLYHLGSRGIEFDLLKWQREHNIPTMAYSPLA
                     QGGRLREQLLESEEVQSIAKKHGVEALQIILAWCLIHPDVVAIPRSSNPDHTYLNAQA
                     ATIRLDDEDLKRLDARFPAPTFKTALDII"
     gene            1067..2464
                     /locus_tag="PLT73_06255"
     CDS             1067..2464
                     /locus_tag="PLT73_06255"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM20016.1"
                     /translation="MSKEALKIVTIGGGSSYTPELIEGYILRKDELPIKEIWLVDIEA
                     GKEKLEIVGAMAKRQVEAAGLDWEVHLTLDRFNALKDADFVTTQFRVGLLNARIKDER
                     IPLSHGVLGQETNGAGGMFKAFRTIPIIGQIIADMKVQCPDAWLINFTNPAGMVTEAA
                     IKHFGWKKTIGLCNVPIGHRKVAAEKLAIPEDELLFKFAGINHFHWHRVWDDKGAERT
                     DEVIDMVYGPQKTEDENHLKNIHSAQFHYEQLKDLGLLPCGYHRYYYIEDEMLKHSIE
                     EFEKGETRAQVVKATETRLFELYKDPALNYKPEELSQRGGTHYSDAACEVIASIQNDK
                     RTDMVVSTANNGTIQDLPYDCVVEVSALITAHGAEPYNWGEMPSAARGLLQGMKAMEE
                     TVIRAAITGSYGAALQAFTINPLVPGGTTAKTLLDELLYAHKEHLPQFAEKIAEIEAN
                     QPETVAYVDELMKSN"
BASE COUNT          799 a          605 c          732 g          678 t
ORIGIN      
        1 tgctacatcc gctatcctta gagccccaag ggctccagaa cagcgtaatt gattcgatgc
       61 tttgcgttta ggtgtggaat tgggaatgac gttgatcgat acagccgaaa tgtacgggga
      121 cgggaagtcg gaacgcttgg ttggggaagc gattaaaggc atccgtgacc aagtctatct
      181 tgtttccaaa gtgtacccgc atcgagcgag ccgaaacgag attttggctg cttgtgccga
      241 aagtttgcgc agattggata cggaccactt ggatctctat ttgctgcatt ggcgaggaaa
      301 tgtgcctttg tcgcaaacga tagccggaat ggagcagttg aaggcggaag gaaagatcgg
      361 cagatggggc gtttccaact tcgacatcca tgatatgcag gatcttttgt cgatggaaca
      421 tgggaaccag tgtcaagtga atcaagtttt gtatcatttg ggctcacgtg ggatagagtt
      481 cgatttgctg aaatggcaac gggagcacaa cattccgacc atggcctact cgccattggc
      541 tcagggaggc aggttgcgcg agcaattgct ggagagtgaa gaagtgcaga gcatcgcaaa
      601 aaaacacggt gtcgaagcac tccagatcat tttggcatgg tgcctcatcc atccagatgt
      661 tgtagcgata ccgcgatctt ccaaccccga tcatacgtat ctcaatgctc aggcggcaac
      721 aatccggctg gatgatgaag atctgaaacg attggatgca cggttcccgg cgcccacttt
      781 caaaacggcg ttagatatca tttaaggatg aaacaaatcg tttcttttct gaaacgaaaa
      841 tcattgtggc aaacctgtaa tcagaatgta aggggattct ccggaagtgt cagatttgtg
      901 ctaaaatgaa ctcatgagag cgaaaagccc tttcacggaa ggttggaatg aaagcctttc
      961 ctaacgggtt gtgcggaact atagccgctt ttttaaggaa tgcattgtca cgacagaatt
     1021 tttgagccgg aaagcaaaga gaaaccaaaa ataggaggaa gcatgcatgt cgaaagaagc
     1081 tttgaagatt gttacgattg gtggaggttc cagttataca ccggaattga ttgaaggata
     1141 cattttaaga aaagatgaat tgcctatcaa agaaatttgg ttggtggata tagaagcagg
     1201 caaggaaaaa ctggaaatcg tcggcgcaat ggcgaaacgc caagtcgagg cggctggatt
     1261 ggactgggaa gtccacttaa cattggatcg tttcaatgct ttgaaagatg cggatttcgt
     1321 aacgacgcaa ttccgtgtag gtttattgaa tgctcgtatc aaagatgagc gcattccatt
     1381 gtcccatggt gttttgggtc aagaaaccaa tggtgcaggc ggaatgttca aagctttccg
     1441 tacgattccg atcatcggcc agatcattgc ggatatgaaa gtgcaatgtc cggatgcttg
     1501 gttgatcaac tttacgaacc ctgcaggtat ggtgactgaa gcagccatca aacatttcgg
     1561 ttggaagaaa acaatcggcc tatgcaatgt tccgatcgga caccgcaaag tcgcagcaga
     1621 aaaattagcg attcctgaag atgaattact gttcaaattt gccggaatca accacttcca
     1681 ctggcaccgt gtctgggatg acaagggggc ggagcgcaca gatgaagtga tcgatatggt
     1741 ttacggaccg caaaaaaccg aagatgagaa ccatctgaaa aatatccaca gtgcgcaatt
     1801 ccactacgag caactgaagg acttaggatt gcttccttgc ggataccatc gttattacta
     1861 tatcgaagat gaaatgttga agcattctat tgaagaattc gaaaaaggcg aaacgcgtgc
     1921 gcaagtcgtc aaggcaacgg aaacacgctt gttcgaactt tacaaagatc ctgctttgaa
     1981 ctacaaaccg gaagaattat cgcaacgcgg cggtacgcac tatagtgatg ctgcctgtga
     2041 agtgattgcg tcgatccaaa acgacaaacg tacggacatg gttgtatcga cagccaacaa
     2101 cggtacgatt caagacttac cttatgattg cgtcgtagaa gtttccgcct tgatcacagc
     2161 tcatggcgca gaaccataca actggggtga aatgccttcc gcggcccgtg gcttattgca
     2221 aggcatgaaa gcgatggaag aaacagtcat ccgcgcggcc atcacaggca gctatggtgc
     2281 tgcattgcag gccttcacaa tcaacccact tgtgccgggc ggtacaacag ccaagacgtt
     2341 gttggatgaa ttgttgtatg cacacaaaga gcacttgccg caattcgcag agaaaatcgc
     2401 tgaaatcgaa gcaaatcaac cggaaacagt cgcttacgtc gatgaattga tgaaatcaaa
     2461 ctaattaaat gagcgtgaaa aagagtggct gccctgtaaa aaaaaggcag ccactttttt
     2521 tgcgcgcaac tccggtgaac gcaggcatca ccggagttcg cccaatattt tgtcttgtgt
     2581 tcttcgatga ggaaggcttc gccggagttg ggacgaatca tcgtctatgt cctccggcga
     2641 gtgatgcctg tccggaggtg cgtctgaatt tccagttgtt tcctcccgcg aatcatcaca
     2701 ctctttttaa acgtgttctg aatcccagaa atctccgcct aagctgcgat tacgtcacgg
     2761 gtaaaaagcg cccgttctcg taatagcctc ttagtgctcg atttctagag cggg
//
LOCUS       DAOMUJ010000228         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10305_length_2799_cov_3.313776, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000228 DAOMUJ010000000
VERSION     DAOMUJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10305_length_2799_cov_3.313776"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PLT73_06260"
     CDS             <1..1914
                     /locus_tag="PLT73_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRM20017.1"
                     /translation="NDVSDIIIATDAGREGELVARWIIEKAKVNKPVKRLWISSVTDK
                     AIKDGFQNLKPGKDYENLYQSAVARSEADWYIGLNATRALTTKFNAQLNCGRVQTPVV
                     AIIARREEEIKKFQPKTYYGIEAQTDTKLKLIWQDEKTNDTKSFERDKVTAIVKKLEK
                     QQATVVAVDRKSKKAYAPGLYDLTELQRDANKLFGYSAKEALNIMQKLYEQHKLLTYP
                     RTDSRYLSNDIIATLPDRLKACAIKEYRPLVNKVLAKPIKANKSFVDDSKVSDHHAII
                     PTEQVVQIGKLSAQELKIYDLVVKRFLAVLFPAYEYEQLTLRAEIGGARFVARGKTVI
                     AAGWKEVYSNRTEDEESEDGLQEQLLPKIEADDVLVVRYVSETSGQTKPPAYFNEATL
                     LTAMENPAKYMETTDKALAQTLKETGGLGTVATRADIIEKLFNAFLIERRGQEIHITS
                     KGKQLLELVPEELKSPALTAEWERKLEQIAAGKLKKDVFINEMKAYTKEIVSEIKMSE
                     GKFKHENISTKTCPECGKPMLEVNGKKGKMLVCQDRECGYRKNVARVTNARCPQCMKK
                     MELHGEGDGQIFTCKCGYREKLSAFNERRKKGTGGKAAKQDVQKYLKKQNQVEEPVNN
                     ALFEALKKLNLGE"
     gene            2070..>2799
                     /locus_tag="PLT73_06265"
     CDS             2070..>2799
                     /locus_tag="PLT73_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM20018.1"
                     /translation="MKTVILAEKSSQAAAYASSFQKKATVAGNYVIQDSLFDGETVIV
                     HASGHLLGLLQPEEYDPKWKKWSLDHLPIFPENYKYKIQYGKGKQMANIKQQLKEADR
                     IIIATDSDREGENIARSIIRHAGAEKKEMKRLWINSLESNEIRRGFSELLDGKDTYPS
                     YIEAQSREIADWLVGMNLSRLYTIALQKAGIKEGAFSVGRVQTPTLFMIYKRMKEISA
                     FQEEIHYECLARITVTNGTFEAKYQ"
BASE COUNT          808 a          700 c          767 g          524 t
ORIGIN      
        1 aatgacgttt ccgacatcat catcgcgacg gatgccggcc gcgagggcga actggtagcc
       61 cgttggatca tcgaaaaagc gaaagtcaat aaacccgtca aacgcctgtg gatctcgtcc
      121 gtgacggaca aggccatcaa agacggcttc cagaacctga agccgggcaa ggactatgaa
      181 aacctgtacc agtcggctgt ggcccgttcg gaagcggatt ggtacatcgg cttgaatgcg
      241 acccgcgcgc tgacgacgaa attcaacgcc cagttgaact gcggccgcgt ccagacacct
      301 gtcgtcgcca tcatcgccag acgcgaagag gaaatcaaaa aattccagcc gaagacatat
      361 tacggcatcg aggcccagac agatacgaag ctgaagctga tttggcagga tgaaaaaacg
      421 aacgacacga agagtttcga gcgcgacaag gtcacagcca tcgtcaaaaa gttggagaag
      481 cagcaggcaa cagtagtggc tgtcgaccgc aaatcaaaaa aagcttatgc accaggcctg
      541 tatgacctga ccgaactaca acgcgatgcc aataagcttt tcggttattc cgccaaagag
      601 gcattgaaca tcatgcagaa actctatgaa cagcataaat tattgactta tccgcgtacg
      661 gattcccgct acctgtcgaa cgacatcata gccacgctgc ctgacagact gaaagcctgc
      721 gccatcaagg aataccgtcc gctcgtgaac aaggtgttgg cgaaaccgat caaggcgaat
      781 aagtcgttcg tcgacgacag caaagtttcc gatcaccatg ccatcatccc gacggaacaa
      841 gtcgtgcaaa tcgggaagct ctcggctcag gaactcaaaa tctatgacct ggttgtgaaa
      901 cgcttcttgg ctgtgctttt cccggcttac gaatatgagc agctgacttt gcgcgcagaa
      961 atcgggggcg cgcgctttgt ggcccgcggc aagacggtca tcgctgccgg ctggaaagaa
     1021 gtctacagca atcgcacgga ggatgaggag tcggaagacg gtctccagga gcaactgttg
     1081 ccgaaaatcg aggcagacga tgtgctggtt gtccgttacg taagcgagac atccggccag
     1141 acgaaaccgc ctgcctactt caatgaagcg actctgttga cggcgatgga aaatccggcg
     1201 aaatatatgg agacgacgga caaagctttg gctcagacgc tgaaggagac gggtggattg
     1261 ggaaccgtcg ctacgcgcgc cgacatcatc gagaagttgt tcaatgcctt cctgatcgaa
     1321 agacgcggac aggaaatcca tatcacttcc aaaggcaagc aactgctgga gttggttccg
     1381 gaagaactga aatcccctgc cctgacagcc gagtgggagc ggaaactcga acagatcgct
     1441 gccggcaagc tgaagaagga cgtattcatc aacgaaatga aggcctacac gaaagagatt
     1501 gtttcggaga tcaagatgag cgaaggcaag ttcaagcacg agaacatctc caccaagacc
     1561 tgcccggaat gcggcaaacc gatgctggaa gtgaacggca aaaaaggcaa gatgcttgtg
     1621 tgccaagacc gcgaatgtgg ataccgcaag aatgtggcgc gggtcaccaa tgcccgttgt
     1681 ccgcagtgca tgaagaaaat ggaactgcac ggcgaaggtg acggccaaat ttttacctgc
     1741 aagtgcggct accgtgaaaa gctatcggcc ttcaacgagc gacgcaaaaa aggcaccggc
     1801 ggaaaagccg cgaagcaaga cgtccaaaaa tatctgaaga aacaaaatca agttgaagag
     1861 ccagtaaata atgccctgtt cgaggcgctt aagaaactca atctgggcga ataagcataa
     1921 ttaggtcgat aaggactgca acggcgcttt ttgtgcctgt tgcagtcctt tttcgatcgg
     1981 gtttcgggaa tgtgcgtatg ttataatcgt aaggtatcga ttgccttaaa agtctcagac
     2041 ggcaatcctg tcagggaggg aatttcagaa tgaaaacagt cattttagcc gagaaatcat
     2101 cgcaggcggc tgcctacgcg agctcttttc aaaagaaggc gacggttgcc gggaactacg
     2161 tcatccagga cagcctgttc gatggcgaaa cggtcatcgt gcatgcctcg ggacatcttt
     2221 tgggactgct gcagccggaa gaatatgatc cgaaatggaa aaaatggagc ctggaccact
     2281 tgccgatttt cccggaaaac tacaaataca agatccaata cggtaaaggc aagcagatgg
     2341 cgaacatcaa acagcagctc aaggaagcgg accgcatcat catcgccacc gactcggacc
     2401 gtgaagggga aaatatcgcc cgctcgatca tccgccatgc tggtgccgaa aagaaggaaa
     2461 tgaaacgtct ctggatcaac agcctggaat ccaatgagat ccgccgcggg ttcagcgaat
     2521 tgctggatgg gaaggacacg tatccatcct acatcgaggc ccaaagccgg gaaatcgccg
     2581 attggttggt gggcatgaac ctgagccggc tgtacaccat tgcgctccag aaggccggaa
     2641 tcaaagaagg tgccttttcg gtaggacgcg tgcagacgcc gacgctcttc atgatctata
     2701 agcgcatgaa ggagatttct gccttccagg aagagataca ttacgagtgt ttggcccgca
     2761 tcacggtcac gaacgggaca ttcgaagcga agtatcagc
//
LOCUS       DAOMUJ010000229         2791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10347_length_2791_cov_2.117325, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000229 DAOMUJ010000000
VERSION     DAOMUJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10347_length_2791_cov_2.117325"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1185
                     /gene="ulaG"
                     /locus_tag="PLT73_06270"
     CDS             121..1185
                     /gene="ulaG"
                     /locus_tag="PLT73_06270"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HRM20019.1"
                     /translation="MANVNEITKESWVLSTFPEWGTWLNEEIEDTVVAENTFAMWWLG
                     CTGIWLKSHESTNILCDLWCGTGKQSHGSGKMKDGHQMQRMSGVQNMQPNLRAQPFVI
                     DPFAVKNVDALVVTHIHSDHLDINTAAAVLKNSSDAVKFVGPQAVVDTWIGWGVPEEK
                     CVVVKPGDSVKIKDIEIVALEAFDRTALVTASEGEVLRGKMPQDMDLIAVNYLFKTSG
                     GNLYHAGDSHYCNYFAKHGNDHQVDVCLGAFGENPRGITDKVTSVDMLRMAESLNAKV
                     VIPVHYDIWSNFQADPKELIELWKFKKDRLQYGFKPYIWQVGGKFVYPNDKDKMEFNF
                     NRGFDDVFSVANDLPFTSFL"
     gene            1213..1680
                     /locus_tag="PLT73_06275"
     CDS             1213..1680
                     /locus_tag="PLT73_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM20020.1"
                     /translation="MLDEVIKNKRFSFHEGFDSWQDSIRAACQPLIEEGAIESAYPEY
                     IIENVNEFGPYIVIAPDICIPHAQEGKGVNKTAICFMRTKVPVNFGPAAEYEARIFFV
                     LASTDNEKHLANLSSLVTLLSDESVVDAFIQANSVADLEKIMEIEGEYLGTIN"
     gene            1661..>2460
                     /locus_tag="PLT73_06280"
     CDS             1661..>2460
                     /locus_tag="PLT73_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRM20021.1"
                     /translation="MLELLISAWTFFQVNILTQPAFFLGIIVFVGYLLLGRPIYDALA
                     GFIKATVGYLVLNVAAGGLVGNFRPILAGLKERFNLSAVVIDPYFGQTAAQQAIEGAG
                     RSFSLMVIVLLIAFMVNIVLVIFRKYTKVRTVFITGHIMVQQSSTALWLVLFAFPELQ
                     DTGVIVMLGLLLGTYWSVFSNLTVEATQDLTEGGGFAVGHQQMFGVWLTDKFAGKFSK
                     NSKKLEDVTLPGFLSIFKESVVSTSILMFLFFGIIMGILGKDLMMTIDL"
     assembly_gap    2461..2470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2471..>2791
                     /locus_tag="PLT73_06285"
     CDS             <2471..>2791
                     /locus_tag="PLT73_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRM20022.1"
                     /translation="KSLYFAVYLQILQLGVRMFVSELTESFQGISNKLLPGSMPAIDC
                     AATYGFGPGNAVTFGFLFGALGQFLAIIGLVVFKSPVLVITGFVPVFFDNATFAVYAN
                     HKGGM"
BASE COUNT          760 a          587 c          675 g          759 t
ORIGIN      
        1 aagcgtttta ttgtatgatt acctcatcga aagaaatgtt cgttatatga acgttttaag
       61 atcggtgtac aaataataag aaaaagtgat cataacgatt acgcagaagg agaatgacga
      121 atggctaatg ttaatgagat tacaaaagaa tcttgggtat tgagtacctt tcctgaatgg
      181 ggcacttggc tgaacgaaga aatcgaggac acagtggttg ccgaaaatac attcgcaatg
      241 tggtggctgg gctgtacagg catttggttg aaatcacatg aatcgacaaa tatcctttgt
      301 gacttgtggt gcggaaccgg caaacaatcc cacggcagcg gtaaaatgaa agacggccat
      361 caaatgcaac gcatgagcgg tgttcaaaat atgcagccta acctcagagc acagcctttt
      421 gtcattgatc ctttcgcagt gaaaaatgtg gatgcattgg tcgttacgca tatccattcc
      481 gatcacttgg acatcaacac agcagcggct gttctgaaaa actcgagcga tgcggttaaa
      541 ttcgtaggac cgcaagcagt tgtggatact tggatcggat ggggagttcc tgaagagaaa
      601 tgcgtcgtcg tgaaaccggg agacagcgtc aaaatcaaag acatcgagat tgttgcgctg
      661 gaagcattcg accgcacagc cttggttacg gcttctgaag gagaagtttt aagaggaaaa
      721 atgccgcagg acatggattt gatcgcagtc aattatttgt tcaaaacatc aggcggaaac
      781 ttgtatcacg caggcgattc ccattactgc aactatttcg caaaacacgg aaacgatcac
      841 caagtggatg tctgcttagg ggcattcggt gaaaacccac gtggaatcac cgataaggtc
      901 acttcagtgg acatgctgag aatggcggaa tctttgaacg cgaaagtagt catcccggtc
      961 cattatgaca tctggtctaa cttccaggcg gatcctaaag aactgattga actttggaaa
     1021 ttcaaaaaag atcgtcttca atacggattc aagccatata tctggcaagt cggcggcaaa
     1081 ttcgtctatc cgaatgacaa agacaaaatg gagttcaatt tcaatcgcgg atttgacgat
     1141 gtgttcagcg tcgcaaatga tttgcctttc acttcattcc tataagaaaa agcacctaag
     1201 aggagagatc atatgttaga tgaagtcatc aaaaataaaa gattttcttt ccacgaggga
     1261 ttcgatagtt ggcaagacag catccgtgct gcttgtcaac cgcttatcga agaaggcgcc
     1321 atcgagagcg cttatcccga gtacatcatc gaaaacgtaa acgaatttgg accttacatc
     1381 gtcatcgcac cggatatttg tatcccgcac gcgcaagaag gtaagggtgt aaataaaacc
     1441 gctatctgct tcatgcgtac gaaagtgccg gtaaacttcg ggccggcagc tgagtacgaa
     1501 gcgcgcatct tcttcgtcct ggcatcgacg gacaatgaga agcacctggc gaatctgtcg
     1561 tcgcttgtga ccttactgtc tgatgaaagt gttgtggatg cttttataca agctaattct
     1621 gttgctgatt tagaaaaaat tatggaaatc gagggtgaat atcttggaac tattaattag
     1681 tgcatggaca ttctttcaag ttaatatctt aacgcaacct gcttttttcc tcggtattat
     1741 cgtattcgtc ggctatctat tgctgggcag acccatatat gacgctcttg ctggattcat
     1801 caaggcaacg gtcggatatt tagtcttaaa tgtagcggct ggcggtttgg tcggaaattt
     1861 ccgtccgatc ctggcaggtt tgaaagaaag attcaactta tcggcagtcg taatcgatcc
     1921 ttattttgga caaacggcag cacagcaagc gatcgagggt gcaggacgtt ccttcagtct
     1981 catggttatc gtgttactca tcgctttcat ggtgaatatt gttttagtca ttttccgtaa
     2041 atatactaaa gtccggaccg tgttcatcac cggccatatc atggtgcaac agtcctctac
     2101 tgctttatgg cttgtcttgt tcgcttttcc tgaattgcag gatacgggcg tcatcgttat
     2161 gttgggcctt ttgttgggaa cgtattggtc agtcttctcg aatctgacag tcgaagctac
     2221 gcaagacttg actgaaggcg gcggatttgc ggtagggcac caacagatgt tcggtgtctg
     2281 gttgaccgac aaatttgcag ggaaattcag caagaacagc aaaaaattgg aagatgtcac
     2341 gctgccaggt ttcctttcca tcttcaagga aagtgtcgtt tcgacaagca ttctgatgtt
     2401 cctgttcttc ggcattatca tgggtatcct cgggaaagat ctgatgatga ccatcgacct
     2461 nnnnnnnnnn aaatcgcttt acttcgcagt ctatctgcaa attttacaac ttggtgtccg
     2521 catgttcgta tctgaattga cggaatcctt ccaagggatc tccaacaagc tgttgcctgg
     2581 ttccatgccg gcgatcgact gcgcggcaac ttatggattc ggaccgggaa atgccgtcac
     2641 attcggcttc ctgttcggag ctttgggaca attcttggcg atcatcggct tggtggtatt
     2701 caagagcccg gtattggtca tcaccggatt cgttccagta ttctttgaca atgcaacctt
     2761 cgctgtatat gcaaaccaca aaggcggtat g
//
LOCUS       DAOMUJ010000230         2776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10406_length_2776_cov_2.700845, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000230 DAOMUJ010000000
VERSION     DAOMUJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10406_length_2776_cov_2.700845"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLT73_06290"
     CDS             complement(<1..309)
                     /locus_tag="PLT73_06290"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM20023.1"
                     /translation="MHYEEAPMRATDEFYRLSQDNDYIKTRKIARNKHFSFDSKYGKL
                     EITINLSKPEKTRQQMEAARATEGGNYPVCALCMENEGYRGRAGAAARSNHRIVRLPL
                     F"
     gene            complement(324..611)
                     /locus_tag="PLT73_06295"
     CDS             complement(324..611)
                     /locus_tag="PLT73_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20024.1"
                     /translation="MQDSSIDQVIRDFVSKGIAAHRIHSLDERYAINHLLGLLEIDVY
                     DIQKIPSIPLPNLLDSLDRMVSYAVEKHLITDVPEEIEIMEAQIMELCLPR"
     gene            815..1747
                     /locus_tag="PLT73_06300"
     CDS             815..1747
                     /locus_tag="PLT73_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HRM20025.1"
                     /translation="MKKTAGIHHITAIVGHPQENVDFYAGVLGMRMIKQTVNFDDPET
                     YHLYFGNADGDPGTVITFFPWAKSRQGSIGDGQVGITVYAVPAGSLSFWEKRLADYNI
                     PFDKSTRFGETYLDFDDPHGLHLELVEREDGNNNPYSFNGVPSEHAIKGFAGAVLLTG
                     APEETAAVVEKVMGLERIGQEDDLIRFRSEAALGNTVDIKMTSVGAGVMGVGTVHHIA
                     WRAKDEADHLDWLKHVTALGQHATPVRDRNYFKALYFREKGGILFEIASDSPGFAIDE
                     PRETMGQKLMLPPQYESRREALTAGLIPFEVRNV"
     gene            1767..2375
                     /locus_tag="PLT73_06305"
     CDS             1767..2375
                     /locus_tag="PLT73_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM20026.1"
                     /translation="MNHQHIFKQGNPERPLLLLLHGTGGTEHDLFFLGEAIDPEASLL
                     GVRGNVTESGMNRYFRRLAEGVFDENDLEYRTKELIAFLDDASAEYGFDRNNIVAIGY
                     SNGANIAGSMIYSFKDAVKGAILHHPMVPFRNRQLPDLHSLPVFIGAGTNDPLCAPEE
                     SIELKATLEGAGSEVAIYWGNAGHSLTNEEVVAAKAWYNKHF"
     assembly_gap    2385..2401
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2402..2673
                     /locus_tag="PLT73_06310"
     CDS             <2402..2673
                     /locus_tag="PLT73_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HRM20027.1"
                     /translation="ELARKAKTADGLTETEKIEQKELRQQYLRSFRSSFDDILLNSKV
                     VDPKGNDITPKKLVEAQKDKRRTDVKNILGGKNVVHLHPEDADKK"
BASE COUNT          727 a          657 c          654 g          721 t
ORIGIN      
        1 gaacaaagga aggcgaacaa tccggtgatt gcttctcgct gccgcaccgg ctctccctct
       61 gtatccttcg ttttccatac aaagcgcgca aacaggatag ttgccgcctt ccgtcgcacg
      121 cgcagcctcc atctgttggc gcgttttttc gggtttggat aggtttatcg tgatttctag
      181 ttttccgtac ttggaatcga acgaaaaatg tttgtttctt gcgattttcc tagttttaat
      241 gtagtcatta tcttgactca aacgatagaa ctcatcggtt gcccgcatcg gtgcttcttc
      301 ataatgcatc caaaaacgct tatttacctc ggaaggcaaa gttccattat ttgcgcttcc
      361 atgatttcga tttcttcggg aacatcggta atcagatgtt tttcaaccgc atagctgacc
      421 atccgatcca gtgagtccag caaattgggc aaaggaatgg aaggtatttt ttgaatatca
      481 taaacgtcta tctctaataa acccaataaa tgattgatgg cgtatcgctc atccaggcta
      541 tggatgcgat gggccgcaat gcctttactg acaaaatccc gtatgacttg gtcgatgctg
      601 ctatcctgca aatttacccc tcattcctct gttgttgtag gtttaattat agcatatgcc
      661 ccgcggtaaa ttttacttct atacttaact ttttatttac cgaccgcttc acgcttacta
      721 tttgacagct tgattttctc gtgttattat tatttcgaat tcgagataat gtgtttactg
      781 aacccaaaac tatttaaaag gagtgtcttt cataatgaaa aaaacagcag gaatccatca
      841 tatcacagcc atcgtgggtc atccgcagga aaacgtcgac ttttatgccg gcgtactcgg
      901 aatgcgcatg atcaaacaga ccgtcaactt tgacgatccg gagacctatc acctttactt
      961 cggcaatgcg gacggcgatc ccgggaccgt catcacgttc ttcccttggg ccaaatcccg
     1021 ccaaggcagt attggcgatg gacaggtggg catcaccgtt tatgcggttc ccgccggatc
     1081 cctgtcattc tgggaaaaac gcctggccga ttacaatatt ccgttcgata aatcgacccg
     1141 tttcggggaa acgtatctgg actttgatga tccgcatggt ctgcatctgg aattggtgga
     1201 acgtgaagac ggaaacaaca atccgtattc cttcaatggc gtaccctcag aacatgccat
     1261 caaaggcttt gcgggagctg tcctattgac gggggcaccc gaagaaactg cagctgtcgt
     1321 cgaaaaagtg atgggcttgg aacgcatcgg gcaggaagat gatctgatcc gtttccgttc
     1381 cgaagctgct ctcggcaata ccgtcgacat caaaatgacc tccgtcggtg ctggtgtcat
     1441 gggcgtcgga accgtccatc atatcgcatg gcgggcaaaa gatgaagcgg atcatctcga
     1501 ttggctgaag catgtcaccg ccctaggaca acatgccact cctgtccggg accgcaatta
     1561 tttcaaagcc ctttacttca gggaaaaagg cggcatccta ttcgagattg cttcggactc
     1621 gccgggattc gctattgatg agccccgcga aacaatgggc cagaaattga tgctgccgcc
     1681 gcagtacgaa agcagacgtg aggccttgac ggcagggctc atcccgttcg aagtccgcaa
     1741 cgtataaaca gagaggattg atttagatga atcatcaaca tattttcaaa caagggaatc
     1801 cggaaaggcc tttgctgctg ttattgcatg gcacaggcgg aaccgaacac gacctgtttt
     1861 tcttaggaga ggcgatcgat ccggaagctt ccttgctggg cgtgcgcgga aacgtgaccg
     1921 aatccggaat gaaccgttat ttccgtcgtt tggcagaagg tgtattcgac gaaaatgacc
     1981 tggaataccg cacaaaggaa ttgattgcgt tcttggatga tgcatctgca gaatatgggt
     2041 ttgaccggaa taacatcgta gcgatcggat actcaaatgg tgcaaatatc gccgggagta
     2101 tgatatactc tttcaaagat gcggtcaaag gagccatcct tcatcatccg atggttcctt
     2161 tccgcaatcg tcaattgcct gatctgcatt cccttcctgt ctttatcggt gcaggcacaa
     2221 acgatccgct ttgcgcaccg gaagaaagca tagaattgaa ggcgacacta gaaggagccg
     2281 gcagtgaggt agccatttat tggggaaacg ctggacactc actgacgaat gaagaggttg
     2341 ttgcagcgaa agcttggtac aacaaacact tctgatctct ccttnnnnnn nnnnnnnnnn
     2401 natgaattgg cccgcaaagc caaaacggcg gatggcctga ccgaaaccga aaaaattgag
     2461 caaaaggaat tacgtcaaca gtatctccgc tctttccgca gttcttttga cgacattctt
     2521 ttgaattcaa aagttgtcga tcccaaagga aatgacatta cacctaagaa attggtggaa
     2581 gcccaaaaag ataaacgccg gactgatgtg aaaaacattt tgggcgggaa gaacgtcgtg
     2641 cacctgcacc cagaggatgc agataaaaag taatgcctac taaaaaacag gactcactcc
     2701 gaattgatgg agtgggtcct gttttttggt gcgggattca tcgtatcctt ttgtaaacgc
     2761 gaatcatcac gctcta
//
LOCUS       DAOMUJ010000231         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10460_length_2768_cov_2.043126, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000231 DAOMUJ010000000
VERSION     DAOMUJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10460_length_2768_cov_2.043126"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /gene="pknB"
                     /locus_tag="PLT73_06315"
     CDS             complement(<1..1639)
                     /gene="pknB"
                     /locus_tag="PLT73_06315"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRM20028.1"
                     /translation="MEAGKKLGGRYKIVRHIGSGGMANVYLGHDLILDRPVAIKVLRF
                     DFRNNTDALRRFQREALSATQLIHPNIVGVYDVDEEDELQYIVMEFIDGTDLKKYIDI
                     QGRVLPEKAIHIMHQVLSAVALAHKNRIIHRDIKPQNILIDNQDRIKITDFGIAVALS
                     ETSITQTNTLLGSVHYLSPEQARGSMATSKSDIYALGVVLYELLSGSVPFDGESAVSV
                     ALKHFQEPMPTIRESHPEIPQSLENVILKATAKEPLDRYATCEEMMADLDTCLSEERR
                     NEAPFMPVSLLQETKVLTPLSAEVVAKADIEKTIISPPQSVKAEAIHEFEPGREAAGG
                     KKKRKKWLFFLFAVFLLSLIGSFFLFFYNNQEADAVTVPDVSNMDQASARLALEDVGL
                     VLGNITEEYNEEIEEDAVIETSPKIGSSVAAGDEIDLIISLGEELFTLLDYEGQEYER
                     VFEDLRKEGFTIERTHEFSSEVAEGNIISQNIEPGTEVKPGETTLSFLVSDGNESYTM
                     RDLSGYTRKSVEDYAGQYGLSLTITEAYSDTIAAGLVV"
     gene            complement(1618..2385)
                     /locus_tag="PLT73_06320"
     CDS             complement(1618..2385)
                     /locus_tag="PLT73_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRM20029.1"
                     /translation="MQIAFKSDRGLNRAINQDYVSYFMNEAGQPLMIVCDGMGGHKAG
                     DVASEMGVSHLGAAWKGSRFTNSEELSRWLIANIQRVNDLVFQKSLDYSDLDGMGTTL
                     VAAAIVGKEIVFANIGDSRAYVYRNYKLRQITEDHSLVNEFIKSGEITPEEAQHHPRK
                     NILTRSLGVSRKLDIDIIAMPLVQGDLLLLCSDGLTNMMDDEEIAQMLKEWLPLEEKV
                     LSLVDKANAAGGLDNITVLLADFTDREGEPLWRQERN"
     gene            complement(2413..>2768)
                     /locus_tag="PLT73_06325"
     CDS             complement(2413..>2768)
                     /locus_tag="PLT73_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRM20030.1"
                     /translation="VDAPCSGLGLMRRKPEIKYVKNAQDFLDLHQEQILILNSVSSLL
                     KKGGRLVYSTCTLTTEENQMSVRTFLENHAEFAIVPIRSEYLDKKSFTAEGFVQIYPH
                     DYGTDGFFISCMVKR"
BASE COUNT          697 a          714 c          549 g          808 t
ORIGIN      
        1 ttacaaccag acctgctgca atcgtgtctg aataagcttc cgtgatcgtc aacgaaagcc
       61 cgtattgccc tgcataatcc tcgacgcttt ttctcgtgta tcccgataaa tcgcgcatcg
      121 tataggattc atttccatca ctcaccagaa atgacagcgt agtttcaccg ggttttacct
      181 ccgtgccagg ctcaatgttt tggctgatga tattgccttc tgctacttcg ctgctgaatt
      241 catgagtcct ttcgatcgtg aagccttctt tacgcaagtc ttcgaataca cgttcatatt
      301 cttggccctc ataatcgaga agagtgaaca attcctcgcc cagactgatg ataagatcga
      361 tctcatcacc ggctgcaacg gatgaaccga tttttggtga tgtttcaatg acagcatctt
      421 cttctatttc ttcattgtat tcttccgtta tatttcctaa taccaacccg acatcttcca
      481 aagcaaggcg cgcactagct tggtccatgt ttgacacatc cggcacagtc acggcatctg
      541 cttcctggtt attgtaaaag aaaaggaaaa aactgccgat caggctcaag aggaacaccg
      601 caaaaagaaa aaatagccac ttctttcgtt ttttcttgcc tccagctgct tctcgccctg
      661 gctcgaattc gtggattgct tcagctttaa cgctctgtgg tggcgaaatg atcgtttttt
      721 cgatatccgc tttagccact acttcagcgg ataaaggcgt aagcactttg gtttcctgca
      781 acagcgatac cggcataaaa ggtgcttcat ttctacgctc ctcgctcaaa caggtatcaa
      841 ggtccgccat catttcttca caagttgcgt aacgatcgag cggttctttg gctgtggcct
      901 tcaggataac attttcaagg ctctgcggta tttccggatg actttccctg atggttggca
      961 tcggttcttg aaaatgcttc agagcgacac tgacagccga ctctccatcg aaagggactg
     1021 atccgctgag caattcatac aacaccacgc ccaaagcgta tatgtccgat ttggaagtgg
     1081 ccatactgcc tctggcctgc tccggggaca agtaatgaac ggatcccaac agcgtattcg
     1141 tctgagtgat tgatgtttcc gacaacgcga ccgcaatacc aaaatcagta attttgatgc
     1201 gatcctgatt atcgatcaga atattttgtg gcttgatgtc cctgtgaata atccgatttt
     1261 tatgtgccaa cgccacagcc gacaaaactt ggtgcatgat atggatcgct ttttccggca
     1321 aaacacgtcc ttggatgtcg atgtattttt tcagatccgt gccatcgata aattccatca
     1381 cgatgtactg caattcatct tcttcatcta cgtcatatac acctacaata ttcggatgaa
     1441 tcaattgcgt ggccgagaga gcttcacgct ggaaacgacg tagagcgtcc gtgttgttac
     1501 ggaagtcaaa tcgcaacact ttgatggcta ctggccgatc cagaatcaga tcgtgtccca
     1561 ggtaaacgtt cgccatccca ccgcttccga tatgcctgac aattttgtag cggccgccta
     1621 atttctttcc tgcctccaca acggttcccc ctctctatcc gtaaaatcag ccaaaagcac
     1681 tgtgatgttg tccaggccgc cagcagcatt cgctttatcg accaaggaaa ggactttttc
     1741 ttcaagagga agccattcct tcagcatttg ggcgatctct tcatcatcca tcatgttggt
     1801 cagtccatcg gaacatagca ataacaggtc gccctgcaca agcggcatgg cgatgatatc
     1861 gatatccagt ttcctggaaa cacccaacga tcgcgtcaaa atgttctttc ttggatgatg
     1921 ctgcgcttct tccggggtta tttcacctga cttgatgaac tcattcacca aagaatggtc
     1981 ttctgtaatt tggcggagtt tataatttct gtagacataa gcccggctat ccccgatatt
     2041 agcaaaaacg atttccttgc ctacgatggc tgcggctacc aaagtcgttc ccatcccgtc
     2101 cagatcgctg taatccaatg atttttgaaa aacaagatca tttacccgct gaatgttggc
     2161 gatcaaccaa cgggacaatt cttcgctgtt cgtgaaacgg gaccctttcc aggctgctcc
     2221 cagatgggat acccccattt cactggcgac gtcaccagct ttatggccac ccataccatc
     2281 acatacgatc atgagcggtt gtccggcttc attcatgaaa taactaacat agtcttgatt
     2341 gattgctcta tttaggcctc tatcactttt gaaggctatc tgcattcttt cccctcaatt
     2401 ctttcttatc atttaccttt ttaccataca gctgatgaaa aatccatcag ttccataatc
     2461 atgcggataa atttgcacga atccttcagc agtgaaagat tttttatcca aatattcgga
     2521 gcgtatcggg acaattgcaa attcagcatg attttcaagg aatgtccgta cagacatttg
     2581 attttcttct gtagtcagtg tacatgtact gtatactaaa cgtccacctt ttttcaacaa
     2641 cgaactgacc gagttcagaa tcagaatttg ttcttgatgc aaatccaaaa agtcctgtgc
     2701 atttttcaca tatttgattt ccggcttccg gcgcatcaat cccagccccg aacaaggtgc
     2761 atcaacat
//
LOCUS       DAOMUJ010000232         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10498_length_2762_cov_1.865903, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000232 DAOMUJ010000000
VERSION     DAOMUJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10498_length_2762_cov_1.865903"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLT73_06330"
     CDS             <1..840
                     /locus_tag="PLT73_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM20031.1"
                     /translation="MTEPKALGVEKEDLIYKFAGLNHFHWHKVADKKGNDVTYKVIDA
                     MFDKDAGLPKNIHDIPFYREQLEQMGMIPCGYHRYYYREEEMLEHGLEEFNGVGTRAQ
                     QVKETEAELFELYKDPNLDHKPEQLQKRGGAYYSDAACETIAAIYANKDTQIVVSTKN
                     NGAVPDLPADCVVEVTAYVGAQGARNVAFGELPTAEKGWLQVMKAMELLTIEAAVTGD
                     YGTALQAFTINPLIRSGETAIKIMNELFIAHKKHLPQFAETIARLEAEGVTVKDEIAK
                     DLD"
     misc_binding    928..1169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1288..1444
                     /locus_tag="PLT73_06335"
                     /pseudo
     CDS             1288..1444
                     /locus_tag="PLT73_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172815.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
     assembly_gap    1445..1455
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1456..2216
                     /locus_tag="PLT73_06340"
     CDS             <1456..2216
                     /locus_tag="PLT73_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM20032.1"
                     /translation="PRTTGDGYKGLLHKQDPTGKSNLIQGIAAVRSLHNRVITETGLT
                     TADEMLYPENLDFVDDLVSYHAIGARSVEDQQHRFVASGIDHPAGMKNPTSGNLKVLF
                     NSVYAAQQKQKFIHNGVEVQSSSNPLAHVVLRGGTNDYGENIPNYHYEDLVKVVKAYE
                     GGDYKNPFIMIDTNHDNSGKKHMEQIRIVKETLFNRSWNDDIKKLARGFMIESYLVGG
                     RQEDDGTVFGQSITDPCLGWEDTEELVKYIADNA"
BASE COUNT          817 a          628 c          696 g          610 t
ORIGIN      
        1 atgactgagc cgaaagcatt aggcgttgaa aaagaggatc tgatttacaa atttgccggc
       61 ttgaaccact tccactggca taaagtcgct gacaaaaaag gcaatgatgt tacttacaaa
      121 gtgatcgatg ccatgttcga caaagatgcg ggccttccga aaaacatcca tgatattccg
      181 ttctacagag aacaattgga acaaatgggc atgatccctt gcggctacca ccgttactac
      241 taccgtgaag aagaaatgct ggaacacggg ctggaagagt tcaatggggt aggcacgcgt
      301 gcgcaacaag tgaaagagac agaagctgaa ttgttcgaat tatacaaaga tccgaacttg
      361 gatcacaaac cggaacaatt gcaaaaacgt ggcggagcgt actactccga tgctgcctgt
      421 gagacaatcg cagccattta cgccaacaaa gacacgcaaa tcgttgtatc gaccaaaaac
      481 aatggcgctg ttcctgacct gccagctgac tgcgtagtcg aagtaacagc ttacgttggt
      541 gcacaaggcg ctcgtaacgt tgctttcgga gaattgccga ctgctgaaaa aggctggttg
      601 caagtgatga aagcgatgga gttgttgacg atcgaagcgg ctgtaacagg cgactacggt
      661 acagcattgc aagcattcac aatcaacccg ttgatccgtt caggcgaaac ggcaatcaaa
      721 atcatgaacg aattgttcat cgcgcataaa aaacacttgc cgcaattcgc tgaaacgatc
      781 gctcgtttgg aagcggaagg cgtgaccgtc aaagacgaaa tcgcaaaaga cttggactaa
      841 ttacgacaag aaaaccctat cattcacttt ttttcaaaaa accatttgac atttccttga
      901 aaaatgagta tattctgaga aacaacataa aaagtgatga taagaagtag tacgttattg
      961 agctgaccag aaaggtgccg gtcgatgcga ggcacacatg cgagatagcc gaattcatct
     1021 ttgaacttcg cactgaatca agtaggctgc gacgggtgat agcgttcgtt atttcgctag
     1081 ggtattataa tgtacctatc gaggtagact tgcgcgagtg gtctatgaac aaaggtggta
     1141 acacgagtct atacggcttc gtcctttcca atgaaaattt ggaatggacg aagctttttt
     1201 tgttgaagag aaaatggagg gattactatg agtttcaaag cattgagtga aaaaatcgat
     1261 tttgaattag tcaaagaatt atccaagctg acgcctgagc aagcagcgat gaaggcagca
     1321 cgcgataaag agttgcacga catcattgaa ggcaaagacg atcgcatcct tttggtcatc
     1381 ggaccttgtt ccgcccacga tgaagaagcg gtaatggaat acgtgcgtcg tttggcgaaa
     1441 ttgcnnnnnn nnnnnaaccg cgtacgaccg gcgatggcta caaagggttg ttgcataaac
     1501 aagatcctac cggcaagagc aacctgatcc aaggaatcgc tgctgtgcgc agcctgcaca
     1561 accgcgtcat cacagaaaca ggcctgacga cagccgacga aatgctgtac cctgaaaacc
     1621 tggattttgt tgatgatctt gtcagctacc acgcaatcgg cgcgcgctcc gttgaggacc
     1681 aacagcaccg tttcgttgcc agcggaatcg atcacccggc cggcatgaaa aacccgacca
     1741 gcggcaacct gaaagtgttg ttcaattccg tttacgctgc ccaacaaaaa caaaaattca
     1801 tccacaacgg cgtggaagtc cagtccagct ccaatccgtt ggcgcacgtt gtcttgcgcg
     1861 gcggcacgaa cgattacggc gaaaacatcc cgaactacca ttacgaagat cttgtcaaag
     1921 tcgtgaaagc atacgaaggc ggcgattaca aaaacccgtt catcatgatc gacacgaacc
     1981 atgacaactc cggcaaaaaa cacatggaac aaatccgcat cgtcaaagaa acgctgttca
     2041 accgttcttg gaacgatgac atcaaaaaac ttgcgcgcgg cttcatgatc gagagctacc
     2101 tagtaggcgg ccgtcaagaa gacgacggaa cagtcttcgg ccaatccatt actgatccat
     2161 gcctaggctg ggaagataca gaagaattgg ttaaatacat cgcggataac gcgtaagagc
     2221 ataaatgcat aaatgtgggg ccaggagaaa tcctggcctt tttgtgtttg ctgtttcgaa
     2281 ggtcggcccg ccgaaggaaa ccgtcagctc cgacgaggga atgttggccg gaggtggttg
     2341 tatttgaggc gactgtactc cggcgaagcc gctggggtcg gaggtaatca tcttgtggga
     2401 tgttctcctc cggcagaaga acagctaacc ggaggaaacc atcagccccg atgagaagtg
     2461 cgctcatcgg agaccagtag tatgccagct tgcaacctcc gacgagggaa tgttggccgg
     2521 aggtggctgt atttgtggcg actgtactcc ggcgaagccg ctatggtcgg aggtaatcat
     2581 tttttggcta atctcctccg gcgagagaat tgctctctgg cagtgccatc agtgcggtaa
     2641 gtgtggccta catttacgtc ccgttggcgc tgacagcggt ttcgttaatt ttcgtctacc
     2701 tctataaatt ggacaaagaa tatgataata ttataaaaga cttaaatgaa cgaaaactgg
     2761 aa
//
LOCUS       DAOMUJ010000233         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10506_length_2760_cov_2.216266, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000233 DAOMUJ010000000
VERSION     DAOMUJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_2760_cov_2.216266"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..745
                     /locus_tag="PLT73_06345"
     CDS             5..745
                     /locus_tag="PLT73_06345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRM20033.1"
                     /translation="MWLGGLPLQLLMTAIGLIAVQELLKMKKLSIFSVEGIITALATV
                     LLMLPESYLQFIPDGIDAMLLIYLEALVLFIFTVFSRNDFTFDDAAASVLTSLYVGRG
                     FYYFMQTRDIGFWMVLLVLFIIWGTDIGAYMIGRKIGKNKLAPAISPNKTIEGSVGGS
                     IAAVLIAIVFFHYYNPLELSLGLQIMLAALLSACGQMGDLVESAFKRYFGVKDAGKIL
                     PGHGGMLDRFDSTLFVMPVFHIFLHFIF"
     gene            782..2056
                     /gene="rseP"
                     /locus_tag="PLT73_06350"
     CDS             782..2056
                     /gene="rseP"
                     /locus_tag="PLT73_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRM20034.1"
                     /translation="MFQTIITFIIVFSILVIIHEFGHFYFAKKAGILVREFAIGMGPK
                     IFSHRKNGTTYTIRMLPIGGYVRMAGIGDEDTELKPGMPLNITLDEEQQVIQIDLSNK
                     QHLQAVPIELLEADLEHALFIEGIIPGSSEPVRYAVKRDATIIETDGTEVQIAPIDVQ
                     YQSAPLLKRMLTNFAGPMNNFILGIAVFIAIAFVQGGVTVNESRLGEIQPDSPAEAAG
                     LRENDEVLAVNGDATADWTELVTSIQANPGNQITLTVVTDGEEARDVLVTPDTKTDEQ
                     GNEYGLIGVAVPMNRTLMAKISYGFKEFWVIATSIFGLVFAMFRTGFQADSFGGPVAI
                     YAATEQVVDYGFLSVLSFLAYLSINLGVVNLLPIPALDGGKLLLNLVEGIRGKPLDPE
                     KEGIITAIGMGLLLLLMVIVTWNDIQRFFFGQ"
     gene            2139..>2760
                     /locus_tag="PLT73_06355"
     CDS             2139..>2760
                     /locus_tag="PLT73_06355"
                     /inference="COORDINATES: protein motif:HMM:NF022927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HRM20035.1"
                     /translation="MSLSKHELFQQMLEQINLHHQPEYLPYFENGEIEQVIVHKKSKL
                     WSFQFVFDNVLPFEVFTALMNHMKIKFQSIATIDFQIKTRKPILTNESILDYWETVVQ
                     RSNISSPLVLSLFAKHTPVVLDNKVVISVENEITKNHLADNYLSIIQQNYMVLGFPSF
                     QITIEVDEAASEARMAQYLARKQEQDEMLVKQAEEAIKQNQQDRQRS"
BASE COUNT          757 a          595 c          672 g          736 t
ORIGIN      
        1 attcttgtgg cttgggggac tgccgctgca actgctcatg acagcaatcg gtttgatcgc
       61 tgttcaagaa ctattgaaga tgaaaaagct gtccatcttc agtgtggaag gtatcatcac
      121 tgctttggcg accgtattat tgatgttgcc ggaatcttat ctgcagttca tccccgatgg
      181 catcgatgcg atgcttttaa tttatctgga ggcgttggta ctgttcattt ttactgtgtt
      241 ctcgcggaac gattttacct tcgacgatgc agccgcttct gtcctgacct ccctttatgt
      301 cgggagagga ttttattatt tcatgcagac aagggacatc ggtttttgga tggtgctctt
      361 agtgttgttc atcatttggg gaacggatat cggcgcatac atgatcggaa gaaaaattgg
      421 caagaacaag ttggctccgg ctatcagccc aaataaaaca atcgaaggat ctgtcggcgg
      481 ttcgatagcc gctgtgctga tcgccatcgt atttttccat tactacaatc cgctggagtt
      541 atctttagga ctccagatca tgctggcagc cttactttcg gcctgtggcc agatggggga
      601 tttggtagaa tcggcattca aacgttattt tggcgtcaag gatgccggga agatattacc
      661 cggacacggc ggcatgcttg atcgttttga cagcacgctt tttgtgatgc cggtgttcca
      721 tatttttctg catttcatct tttaacaaag cgaagacacg ctacaaaaag ggagggatta
      781 catgtttcag acaatcataa cgttcatcat cgtgttcagc atattagtga tcatccacga
      841 attcggccat ttctattttg cgaaaaaagc tggcattctg gttcgtgaat tcgcaatcgg
      901 catgggaccg aaaatcttct ctcatcgcaa aaacgggaca acctacacaa tccggatgct
      961 gcccatcggt ggttatgtgc ggatggccgg catcggggat gaggacaccg agttgaaacc
     1021 gggtatgccg cttaacatca cccttgatga agagcaacag gtcattcaaa tagatttgag
     1081 caacaaacag catttgcagg ctgtgccgat agaattactg gaggctgatc tggaacacgc
     1141 acttttcata gaaggaatca ttccgggaag ttcagaaccc gttagatacg cagtaaaacg
     1201 cgatgcgaca atcatcgaga ccgacggaac agaagttcag atcgctccca tcgatgtaca
     1261 ataccaatca gcacctttgc tgaaacggat gctgacaaac ttcgccggac cgatgaataa
     1321 tttcattttg ggtatcgcgg tattcatcgc gattgcgttc gtgcaaggcg gcgttacagt
     1381 aaacgagagt cgcttgggag aaatccaacc tgacagcccg gcggaagcag ccggattgcg
     1441 ggagaatgac gaagtattgg ctgttaatgg tgatgcaacc gctgactgga cggaactggt
     1501 tacgtccatc caagccaatc cgggcaacca aatcacactt acagtcgtga ctgatggaga
     1561 agaagcgcgt gatgtcctcg tcacacctga cacgaaaact gatgaacaag gcaacgagta
     1621 tggtttgatc ggtgtcgctg tcccgatgaa tcgcacgctg atggctaaaa tcagctacgg
     1681 attcaaagaa ttttgggtga ttgccacttc cattttcgga ttggtctttg ccatgttcag
     1741 gaccggcttt caggctgact cattcggcgg gcctgtcgct atttatgcgg ccaccgagca
     1801 agtcgttgat tatggctttc tgagcgtact cagttttctg gcctatttga gcatcaacct
     1861 tggtgtcgtg aatctattgc cgattccagc attggatggc gggaaactac tgctgaacct
     1921 agttgaagga atcagaggca agccgttgga tccagagaag gaaggcatca tcacggctat
     1981 cggaatgggg ctgttgcttc tgctgatggt catcgtcact tggaatgata ttcaaagatt
     2041 tttctttggc caatgatagg aagaaaataa gaagattcaa agaaaggaac gttcattctt
     2101 tgaatctttt tgtttgttag ttgagaagga gagatcaaat gtcgttatcg aaacatgaat
     2161 tgttccagca gatgttggaa cagataaatt tgcatcacca gccggagtat ctaccgtatt
     2221 ttgaaaacgg ggaaatcgaa caagtcatcg tgcataaaaa atcaaaatta tggtcatttc
     2281 agttcgtttt tgataacgtt ctgcctttcg aagtgttcac tgctttgatg aaccacatga
     2341 aaataaaatt ccaatccatc gccacaatcg attttcagat caaaaccagg aaaccgattt
     2401 tgacgaatga gagcatcttg gactattggg aaacagtcgt ccaacgcagc aatatatcat
     2461 caccactggt tctgagtctt tttgccaaac atacacccgt tgttttggac aataaagtcg
     2521 ttatcagtgt tgaaaacgaa atcaccaaaa atcatttggc ggacaactac ttgtccatta
     2581 tccaacagaa ttacatggtc ttgggatttc cgagtttcca gatcacaatc gaagtggacg
     2641 aggcagcatc cgaagccaga atggcacaat atctggcccg aaaacaagaa caggatgaaa
     2701 tgttggtcaa acaagcagag gaagccatca aacaaaatca gcaggataga cagcgcagcg
//
LOCUS       DAOMUJ010000234         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10619_length_2738_cov_1.945583, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000234 DAOMUJ010000000
VERSION     DAOMUJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10619_length_2738_cov_1.945583"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..629
                     /locus_tag="PLT73_06360"
     CDS             198..629
                     /locus_tag="PLT73_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HRM20036.1"
                     /translation="MKMEYKIGDIIEGNVTGIQSYGVFISLDKNTQGLIHISECRHGY
                     VTNLDEFIQIDDKVEAKIIDIDEYTGKISLSLRAMEKLATPCYPAKRKKRKRRYLPQI
                     GFETLKEMMPTWIEEAKEDEKVRKSTGNNPVYTTSDERGHS"
     gene            626..1012
                     /locus_tag="PLT73_06365"
     CDS             626..1012
                     /locus_tag="PLT73_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20037.1"
                     /translation="MMIKENIVAGTVMVTNDAEETYFLVNTENGTYSLPNVNLSESGK
                     SPLGSIMEVLLAHIGIESESLRLLDLTNMRGNGKNMPLFVFDSLEKPSDLDSLLNEPG
                     KFAWKKSSEISEILTDLELDSVPIYH"
     gene            1069..2415
                     /locus_tag="PLT73_06370"
     CDS             1069..2415
                     /locus_tag="PLT73_06370"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRM20038.1"
                     /translation="MSHIQFDYSKALKFFAQHELDYIQYQVTAADKALREGTGPGSDF
                     TGWIDLPKDYDKEEFARIKAAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNNTFYNV
                     QTTADRKTPQIFFAGNSISSTYLADLIEVIGDRDFSVNMISKSGTTTEPAIAFRVFKE
                     LLIKKYGKEEAVKRIYSTTDKARGAAKHEADMEGYETFVIPDDVGGRFTVLTPVGLLP
                     IAVSGADIDALMQGAADAREAYMNPDLTTNVAYQYAAIRNILYRKGKVTEILANYEPG
                     MQYLSEWWKQLFGESEGKDQKGIYPTSANFSTDLHSIGQSIQDGQRNIFETVVKVAKP
                     RKTIDIPLSAEDLDGLGYLEGKDVDFVNTKAYQGTLLAHTDGGVPNLLVTMPEMDAYS
                     LGYLVYFFEIAVGISGYLNGVNPFDQPGVEAYKKNMFALLGKPGFEELAKELNDRL"
BASE COUNT          860 a          520 c          612 g          746 t
ORIGIN      
        1 aggggctgaa agagtgcccc tttttctact tgaatgagat tatcctattt ccgtcactcc
       61 tgacattgct ggagtgtttt cagagtaggg atcacaaagc cgcctaagag ttgcttctga
      121 ttaaagttgc atcagaaatt ctatcctttc atttaggcac gtggtataat tattcggtac
      181 tgtgagggga attatggatg aaaatggaat ataaaatcgg agacataatt gaaggcaatg
      241 taacaggtat ccaatcatat ggtgtcttta tttcattgga caagaatacg caaggcctga
      301 tccacatatc cgagtgtagg catggatatg tgaccaacct ggacgaattc attcagatcg
      361 atgataaagt agaagccaaa attatagaca ttgatgagta cacagggaaa atcagcctgt
      421 ctctcagggc tatggaaaag ttggccacac cgtgttatcc ggcaaaaaga aagaaaagaa
      481 aaagaagata cttaccgcaa atcggttttg agacattgaa agaaatgatg ccgacctgga
      541 tcgaagaggc caaagaagac gagaaagtta ggaaatcgac gggaaataat cctgtctata
      601 cgacttccga tgaaagggga cattcatgat gataaaagaa aacatagtgg cagggactgt
      661 tatggtcaca aatgatgcag aggagacgta ctttctggtc aatacagaaa acggaaccta
      721 cagcctacct aatgtgaact tatccgaaag tggaaaatct cccttaggat ccataatgga
      781 agtgttactg gcgcatattg gcattgaatc tgagtcgttg cgattgcttg acttgacgaa
      841 catgagagga aatggcaaaa acatgccttt gtttgttttc gattcattag aaaaaccttc
      901 tgatttggac agcttgctga atgaacccgg caagttcgca tggaaaaaat cttcagaaat
      961 ctcggaaata cttactgatt tggaattgga cagtgtacct atctaccatt aaggtgcctt
     1021 atttcccatc agtagtcatt ttataaaaac aaattaggag gatattaaat gtcacatatt
     1081 caatttgatt actctaaagc tttgaaattt tttgcacaac atgaattgga ctacatccag
     1141 taccaagtaa cagccgctga caaagcgctt cgtgaaggta caggcccagg gagcgacttc
     1201 actggctgga tcgatctgcc taaagattat gataaagaag aattcgcgcg catcaaagcg
     1261 gcagcgaaaa aaatccaatc cgattcagaa gttttggttg ttatcggtat cggtggttct
     1321 tatttaggcg cccgtgctgc aattgacttc ctgaacaaca ctttctacaa cgttcagaca
     1381 actgcagacc gcaaaacacc acaaattttc tttgcaggaa acagcatcag ctcaacttat
     1441 ttagctgact tgatcgaagt gatcggagat cgtgatttct cagttaacat gatttcaaaa
     1501 tcaggtacaa caactgaacc ggcgatcgct ttccgtgtct tcaaagaatt gttgatcaaa
     1561 aaatatggta aagaagaagc agtaaaacgc atttactcta ccacagacaa agcaagagga
     1621 gcagcgaagc atgaagcaga catggaagga tacgagactt tcgtaattcc tgatgatgtc
     1681 ggcggacgtt tcacggtgtt gacaccagtt ggccttcttc cgatcgctgt cagcggtgct
     1741 gatatcgatg cattgatgca aggtgctgct gatgcccgtg aagcatacat gaatcctgat
     1801 ttgacaacca acgttgctta ccaatacgcg gctatccgca acatccttta ccgtaaaggc
     1861 aaagtgactg agattttggc taactatgag ccaggtatgc aatacctttc agaatggtgg
     1921 aaacaattat tcggcgagtc tgaaggtaaa gaccaaaaag gtatttatcc aaccagcgcg
     1981 aacttctcga ctgatttgca ttcaatcggc cagtccatcc aagatggaca aagaaatatc
     2041 tttgagacag tcgttaaagt tgctaagcca cgcaaaacaa tcgatattcc tttaagtgca
     2101 gaagatttag atggtttagg ctatcttgaa ggtaaagacg ttgatttcgt aaacacaaaa
     2161 gcttaccaag gaacattgtt ggctcataca gacggcggcg taccgaactt gttagtcact
     2221 atgcctgaaa tggatgctta ttctttaggt tatctagtgt acttctttga aatcgccgta
     2281 ggcatctccg gttacctgaa cggcgtgaat ccattcgacc aaccaggtgt agaagcttac
     2341 aaaaagaata tgtttgcact tcttggcaaa cctggatttg aagaattagc taaggaatta
     2401 aacgacagat tataagatgt aaattacttc aagcggacaa ttcgttgtcc gctttttttc
     2461 ttgtgtagca gttcagaacc ctaagggcgg aggaattcga atcgatcttg tcaggacttc
     2521 cggagaggag ggcttctctg gagcagcaca accaaaaaag ttagttgtta aacaattcaa
     2581 aattggatgt cggatccata aggatgctga tacgcttgtg ttgacaaggc ttacattttt
     2641 gtggtaaagt ttagttcctc ggtaatccga tacatttttt tcaaaatctg tttgacatgg
     2701 atttgaaatg atgatagtat tgtgaagttg tcttttgt
//
LOCUS       DAOMUJ010000235         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10628_length_2736_cov_1.754196, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000235 DAOMUJ010000000
VERSION     DAOMUJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10628_length_2736_cov_1.754196"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLT73_06375"
     CDS             <1..1083
                     /locus_tag="PLT73_06375"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRM20039.1"
                     /translation="GMPIGGVIAAEKVIIPNAVGVDIGCGMSFVETNVPVERLIEIET
                     PNGKLAQAIAGDILRNIPTGFEHHKKKQQCESVARFLEGMTPEEKDTMPQELMKELDD
                     AAYQVGTLGSGNHFIELQTDDQGKLGIMLHSGSRNLGFKICHYFNDLAKEVNKQEQSS
                     VPPEWDLAYFSTESDLGKLYLRWMKLAMDFAEENRNQMMEKVLDIVRLWVKKYAGINH
                     FKLSDAVHCHHNYAALEEHYGKEVWVYRKGAIRAGAGEMGIIPGAMGTYSYLVKGKGN
                     PESFLSCSHGAGRRMSRLYAREQFSVQDTILDLRELGVFLAKAKRTDVGTESRFAYKA
                     IDFVLSQELDLVEPVRRLKTVAVVKG"
     gene            1103..2080
                     /locus_tag="PLT73_06380"
     CDS             1103..2080
                     /locus_tag="PLT73_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20040.1"
                     /translation="MELQERYIAAGLKHIPPTEKDGVEKAMRRIIAERLQERGNASEE
                     TEREVLRGLGSPRILAEKQLREPPHLIGPELYGTYMLIVKIVMTVAVIGTLIGNTVDF
                     IVNGETILRYIAQSFAAAIGVAIGAFGWVTLVFAIMERTAKQKILTEIQEDWSLADLP
                     EKEVPQKPFNKVGVIIGIIFTVLFIILVNQYSQLLGFYYTLDGSIQEMIPMLNQEVFR
                     SYLPYINGMLVLQLLFSASKLVFRKWTYPVATANLILNVLSFVLLWFILQDTAILNPE
                     LVTKIGEATDGQRVLNTAFNSIKAVFLFIFLLDSFEGFHDAYKNSKKPA"
     gene            2174..2545
                     /locus_tag="PLT73_06385"
     CDS             2174..2545
                     /locus_tag="PLT73_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20041.1"
                     /translation="MEQTTAKEYILYNEVTNEYLTRVTFMGSTVYKTTTLDLALRFSE
                     QDKESLEAMGAGDPEKVLNNIEATAFGNDSDVKETYRLSNLSFLSINDLTPEERAAFD
                     QEIEEGKKKAARIHCCRIVSR"
BASE COUNT          743 a          680 c          756 g          557 t
ORIGIN      
        1 ggcatgccga tcgggggcgt catcgctgcc gaaaaggtca tcattccgaa tgcggtcggg
       61 gtggatatcg gctgtgggat gagttttgtc gagacgaacg ttcctgtcga acggctgatc
      121 gaaatcgaga cgccgaacgg gaagctggcc caggcgatcg cgggcgacat cctgcggaat
      181 atcccgaccg gcttcgagca ccataagaaa aaacagcaat gtgaatcggt cgcccgcttc
      241 cttgagggaa tgacacccga ggaaaaggac acgatgccgc aggaattgat gaaggaactg
      301 gacgatgccg cctatcaagt cggcacgctc ggcagcggca accatttcat cgaactgcag
      361 acggacgatc agggaaagct tggcatcatg ctgcattccg ggtcgcgcaa tctgggcttc
      421 aaaatctgcc attatttcaa cgatctagcc aaggaagtga acaaacagga gcagtcttcg
      481 gtccctcccg agtgggatct ggcctatttt tccaccgaaa gtgatctcgg gaaattgtac
      541 ctgcgctgga tgaaattggc gatggatttc gccgaagaaa accgcaatca gatgatggag
      601 aaggtactcg atatcgtccg actctgggtg aaaaagtacg ccggcatcaa ccacttcaag
      661 ttatcggatg ccgtccactg ccaccacaat tatgccgcac tggaagaaca ctatggaaaa
      721 gaagtctggg tctaccgaaa aggggccatt cgcgccggtg caggcgaaat gggcatcatc
      781 ccgggcgcga tgggaaccta ttcctatctg gtcaaaggca aaggcaaccc ggaaagcttc
      841 ctttcctgtt cgcatggggc gggtagacgc atgagccgtt tgtatgccag ggaacagttc
      901 agtgtccagg acaccattct ggatttgagg gaattgggcg tctttctcgc gaaagccaaa
      961 cggacagacg tcggcacgga atcgcgtttt gcctacaagg ccatcgactt tgtgctctca
     1021 caagaactgg atctcgtcga accggtccgc cgcctcaaga ccgtcgcagt agtcaagggc
     1081 tgaaaagaaa agagggacaa aaatggaact gcaggaaagg tacatcgcgg ccggcctgaa
     1141 acatatcccg ccgaccgaaa aagatggagt ggaaaaggcc atgcgccgca tcatcgcgga
     1201 gcggctgcag gaacgaggca atgcttccga agagacggaa cgcgaagtgt tgagaggatt
     1261 ggggagtcca aggattctgg ctgaaaagca attgagggaa ccgcctcacc tgatcggtcc
     1321 ggaactgtac ggcacctaca tgttgattgt taaaatcgtc atgacggtgg cggtcatcgg
     1381 cacgctgata gggaataccg tcgatttcat cgtcaacggc gaaaccatat tgcgttatat
     1441 cgcccaaagt ttcgcagcag caatcggtgt ggcgatcggg gctttcggat gggtgacgct
     1501 cgtgttcgcc atcatggaaa ggacggcaaa acagaagatc ctgacggaaa tccaggaaga
     1561 ctggagtctg gctgatctgc ctgagaagga agttccgcag aaaccgttca acaaagtcgg
     1621 ggtcatcatc ggcatcatct ttacggtgct gttcatcatc ctcgtcaacc aatacagcca
     1681 gctgctggga ttctactata cgctggacgg cagcatccag gaaatgattc cgatgttgaa
     1741 tcaggaagtg ttccgcagtt acttgcctta catcaacggc atgctggtgc tgcaattgct
     1801 gttctccgcc agcaaactcg ttttcaggaa gtggacctat ccggtcgcga ccgccaatct
     1861 gattttgaat gtactttcgt ttgtattgtt gtggttcatc cttcaagata cagccatcct
     1921 gaatccggag ctcgtcacga aaatcggcga agccacggat gggcaacgtg tcctcaatac
     1981 tgcattcaac tcgatcaaag ccgtcttcct gttcatcttc ctgctggact cattcgaagg
     2041 tttccatgat gcctacaaaa acagcaaaaa gccggcttga accggtaaac tattacggcc
     2101 ggtttgtgaa aaacagagcg gatggtctat aatgggaatg taaatcggta tttttggagg
     2161 gaacaactga atcatggaac aaacaacagc taaagaatac atcctttaca acgaagtcac
     2221 gaacgaatac ctgacgcgcg tcaccttcat gggcagcacc gtctacaaga cgacgacgct
     2281 cgacttggcg ctgcgtttct ctgaacagga caaggagagc ctggaagcga tgggagccgg
     2341 cgatccggaa aaggtgctga acaacatcga agcgacggct ttcggaaacg acagcgatgt
     2401 gaaggaaaca tacagactgt ccaatctgag cttcctgtcg atcaacgact tgacgccgga
     2461 ggaaagggcg gccttcgatc aggaaatcga agaaggtaaa aagaaagcag cccggatcca
     2521 ctgctgccgg atcgtcagca gataaaccgc caagtcggaa caagcagcaa tcaatcaaat
     2581 cagtcattat tcaggacaga gcagtaggca aattttcagg tcaggttgca caagccagcc
     2641 ccgtaaaaag gttgctgctg acttcgaatg acactcctcg gcgattggtc ggggaacgcc
     2701 accgaaaagg caaaaacacg ggcgttatgc aatcct
//
LOCUS       DAOMUJ010000236         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10669_length_2729_cov_2.317128, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000236 DAOMUJ010000000
VERSION     DAOMUJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10669_length_2729_cov_2.317128"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLT73_06390"
     CDS             complement(<1..838)
                     /locus_tag="PLT73_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRM20042.1"
                     /translation="MSRRTSLIVALVASLALLLFVSANLFSTFFNSGDNRVTTIDTDS
                     SSTGNLSGMGHMMGGEQSVVLDESGQPMTALQLPELLEPDSESASEITYTVTAQAGET
                     AFLEGKTTQTLGYNGSYLGPVLVVREGQEVHIKTENQLAEATSFHWHGVKLPSDMDGG
                     PHHPVGPNETMQVDFTVNQEAATLWFHPHALGTTAEQVYAGLAGLIFVEDDNSDQLDL
                     PKDYGVNDFPLIIQDRTFDADNQFDYAGTYNPDGTYGDTLLINGTINPYVEVTNELVR
                     LRL"
     gene            complement(993..2123)
                     /locus_tag="PLT73_06395"
     CDS             complement(993..2123)
                     /locus_tag="PLT73_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRM20043.1"
                     /translation="MIQALTDLIRMSGLAHLTYKEIIMIIIACIFLYLAIKKGYEPYL
                     LIPIAFGILLVNLPLAGLMTPPTESEPGGLIYYLYQGTNLGIYPPLIFLCLGAATDFG
                     PLIANPKTLLLGGAAQFGIFAAFFGALALGMTPQEAASVGIIGGADGPTAIYLTTKLA
                     NHLLSVIALAAYSYMALVPLIQPPIMRLLTTEKERQTVMKQMRVVSKNERILFPIVTT
                     IFVSLVVPSATALVGMLMLGNLIRESGQVPKLTETLQNAMMYIVTILLGVTVGAKANG
                     ELFLSLTTIKIIVLGLFAFSIGTAAGVLMGKLMYKLTNGKVNPLIGAAGVSAVPMAAR
                     VVQKEGIKYNPSNYLLMHAMGPNVAGVIGSAVAAGVLLAFFG"
     gene            complement(2140..2487)
                     /locus_tag="PLT73_06400"
     CDS             complement(2140..2487)
                     /locus_tag="PLT73_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRM20044.1"
                     /translation="MKKYEIEIDGQVYHVKVRELPDDAVMREQPKAGSGKSAADAAPQ
                     TEGKTMLAPMAGTILRILVKEGQRVKKGENLIVLEAMKMENEIVADEDGVIRSILVKA
                     NDSVESDQPLLIL"
     gene            complement(2522..>2729)
                     /locus_tag="PLT73_06405"
     CDS             complement(2522..>2729)
                     /locus_tag="PLT73_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20045.1"
                     /translation="VELVHKLVGEPAPATTAPIDREEASQPVLLDPGVDEQRAKAAAI
                     TALVAAYEAHPAKKFEIVDVTRIK"
BASE COUNT          667 a          761 c          638 g          663 t
ORIGIN      
        1 gcagacgcaa ccggaccagt tcatttgtga cttccacata gggattgatg gtcccgttga
       61 tcagcaaggt atcgccatag gtaccgtcag gattgtaagt ccctgcgtaa tcgaattgat
      121 tgtccgcatc gaaggtccgg tcctggataa tcagagggaa gtcattcacc ccatagtcct
      181 tcggcaaatc caattgatca gaattgtcgt cctcgacaaa gatcaatccg gccagaccgg
      241 catagacctg ttcagccgtc gttcctaagg catgcgggtg gaaccacaac gtcgctgcct
      301 cctgattgac cgtgaaatcc acctgcatcg tttcattcgg tccgaccgga tgatgcggcc
      361 caccatccat atcagaaggc agctttacgc catgccaatg gaaagaggtt gcttcggcta
      421 actggttctc ggtcttgata tgcacctctt ggccttcacg cacgaccaaa accggtccaa
      481 gataagaacc attataaccg agcgtctgcg tcgttttccc ttccaagaag gcagtttccc
      541 ccgcttgcgc tgtgaccgta taggtgattt cgctagcgga ttcggaatcc ggttccaata
      601 gttccggcaa ctgcagtgca gtcattggct gcccactttc atccagtacg acagactgct
      661 ctcctcccat catatgcccc atcccgctaa gattccctgt gctgctcgaa tccgtatcaa
      721 tagttgttac ccgattatcc ccgctattga aaaacgtcga gaaaagattg gctgatacaa
      781 acagcaacaa cgccagcgaa gcaaccaaag cgacaatcaa cgaggttctc ctgctcattt
      841 taatccgtcc ttcctttctg actcttttgt tgccttcatt atacatcctt catgtgtaga
      901 acagatggat ttagcaatcg tgtagcctcg aaacaataaa aaaatcccct tctgacatga
      961 aacatcagaa ggagatgatt aataatccac actcaaccga agaaagccaa caggacaccg
     1021 gccgcgacag ccgatccgat gacgcccgcc acattcggtc ccatcgcatg catcagcaaa
     1081 tagttggaag gattgtattt gatgccttcc ttctgcacaa cccgcgcagc catcggtacg
     1141 gccgaaacac cggctgcccc gatcaacgga ttcactttac cgttcgtcaa tttgtacatc
     1201 agtttaccca tcaggacgcc ggcagcggtc ccaatcgaga aagcaaaaag gccaagaacg
     1261 atgattttta tggttgtcag cgacaagaac aactcgccgt tcgccttggc tccgactgtt
     1321 acgcccaaaa gtatggtgac gatgtacatc atggcattct gcagcgtttc cgtcagtttc
     1381 ggaacctgac cgctttcccg gatcagatta cccagcatca gcatccccac aagcgctgtg
     1441 gctgatggga cgaccaagct gacgaagatc gtggtgacga ttgggaacag gatacgctcg
     1501 ttcttggaca ctacgcgcat ctgcttcatg acagtctgcc gctctttttc ggtcgtcaac
     1561 aggcgcatga tcggcggctg gataagggga accaaggcca tataggaata ggccgccagg
     1621 gcgatgaccg ataagagatg gttggccaat tttgtcgtca aatagatggc agtcggtccg
     1681 tccgccccac ctatgatgcc gacggatgcg gcttcctggg gcgtcatccc caaggccaag
     1741 gccccgaaga atgccgcaaa gatgccgaac tgtgctgccc cgcccaacaa caacgttttc
     1801 ggattggcga tgagcggccc gaaatcggtc gctgccccca gacagaggaa gatcaacggc
     1861 ggatagatgc ccagattagt accttggtag aggtaataga tcagccctcc cggttcgctt
     1921 tcggtaggcg gcgtcatcaa tccggctaac ggaagattca ccaacaggat gccgaacgca
     1981 atcggaatca acaaataggg ctcatagccc tttttgatgg ccaaataaag gaaaatgcag
     2041 gcaatgatga tcataatgat ttctttatac gtaaggtgag ccaatccgga catccggatc
     2101 agatcagtca gagcttgaat cataggaatc cctcctcttc tagagaatca acaatggttg
     2161 gtcggattcg acactgtcat ttgctttcac caaaatactt ctgatgacgc catcctcatc
     2221 tgcaacgatc tcattttcca tcttcatggc ttcgaggacg atgaggtttt cgcctttttt
     2281 gacccgttgc ccctctttga cgaggatgcg caagatggtg cccgccatag gggccaacat
     2341 tgtttttcct tctgtttggg gggcggcatc agcagcgctt ttgccgctgc cggccttagg
     2401 ttgctctctc atgactgcat catccggcaa ctcccttact ttgacatgat acacttgtcc
     2461 gtcgatttcg atttcatact ttttcataaa aaatcctccc tttataatca gcagatggaa
     2521 tttatttgat acgcgttaca tcaacaatct cgaacttctt ggccggatgg gcttcataag
     2581 ccgcgaccaa tgccgtgatt gccgctgctt tagctcgttg ctcatccaca cccggatcca
     2641 gcaggacggg ctgactagct tcttcgcgat caatcggtgc tgtagttgcc ggtgctggtt
     2701 ccccgaccag cttatggacc agctcaacc
//
LOCUS       DAOMUJ010000237         2728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10680_length_2728_cov_2.510662, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000237 DAOMUJ010000000
VERSION     DAOMUJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10680_length_2728_cov_2.510662"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..992)
                     /locus_tag="PLT73_06410"
     CDS             complement(99..992)
                     /locus_tag="PLT73_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM20046.1"
                     /translation="MWFLFAIVSVLAWGTADLFYKKGSDPKDKYSYLKIVIMVGAVMG
                     IHAMYVLLTSGVVYEPRNMITYLPVSALYILSMAVGYAGLRFLELSISSPVQNSSGAI
                     SGLLTFIFLGQSMTGIQFFAVGLITVGVILLSVFEQRFAEAERKENKEMVDRKYKYGA
                     IALLFPLGYALIDSLGTFADAWVFDRGMDEMQANISYELTWLIVAVLAWVYLVWIKKE
                     TFALRDQKDRGLAAIFETLGQFFYVFAISANAVIVAPLISSYSMVSVILSRIFLKEKL
                     TAKQYAVIAMIMVGIFILGFD"
     gene            complement(1139..1639)
                     /locus_tag="PLT73_06415"
     CDS             complement(1139..1639)
                     /locus_tag="PLT73_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRM20047.1"
                     /translation="MTLTHRNATLDDVEDIVAIYNQTIPSRMVTADLEPVTVKQKMGW
                     FLDHSPSYRPIWVFEEDGKICAWVSFQAFHERAAYDKTAELSIYIHEGYRGKKMGTQI
                     METVLAACDELDITNLVCLIFGHNAPSIGLMKKFGFEQWGYLPRVAELDGIERDLVYM
                     GKRVRD"
     gene            1853..2551
                     /locus_tag="PLT73_06420"
     CDS             1853..2551
                     /locus_tag="PLT73_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM20048.1"
                     /translation="MNEEIYIAEDEDGIRLTVKTFLESEGYAVSDFPNGDLLYDAFLD
                     KQPDLVILDVMMPGSSGFEITKKIRDISSVPIIILTAKDSDMDYATGINLGSDDYFTK
                     PFSAIALNMRVKAMLRRIKMDQERLPARQEEATLRIGDIEIDLAAMSVKQNGVSLNLT
                     PNEYNVFVYLIENKNRAVSRDELLDSIWGYGKDIETRACDDTVRRLRKKLAGSTVEIE
                     TVWGFGFTVKESSA"
     gene            2548..>2728
                     /locus_tag="PLT73_06425"
     CDS             2548..>2728
                     /locus_tag="PLT73_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20049.1"
                     /translation="MMKMKTSKKSLKIRLLTTVTLLLAAIFTLIYLVFNFFFTQYIET
                     AATSLLSEARQDYIER"
BASE COUNT          780 a          680 c          586 g          682 t
ORIGIN      
        1 ataattatcg cttatttcag cagaaactac aaaaaagaag cgtgttatag agcgccacaa
       61 cgcaaaaaag cggcattcag ccgctttttt gcataaaact aatcaaaccc cagaatgaag
      121 atccctacca taatcattgc aatgaccgcg tattgttttg cggtcagttt ttctttcagg
      181 aaaatccgtg aaaggatgac ggataccata ctataggaag aaatcagcgg cgccacgata
      241 actgcattcg cactgatcgc aaacacatag aagaactggc ccaacgtttc aaatatggct
      301 gccaaaccgc ggtctttctg atcgcgcaag gcgaatgtct ccttcttgat ccagacaagg
      361 taaacccagg ccagaacggc aacaatcagc catgtcaatt cataggagat gttcgcctgc
      421 atttcatcca ttcctctgtc gaatacccac gcgtccgcga acgtcccaag tgaatcgatc
      481 aatgcatatc ccaatggaaa cagcaaagcg atcgcgccgt atttatattt gcggtccacc
      541 atttctttgt tctcttttcg ttcggcctcg gcaaatcgtt gctcaaaaac cgacagcagg
      601 ataacaccaa ctgtgattag ccctactgca aaaaattgga ttcctgtcat ggattgcccc
      661 aggaaaataa aagtcaacaa acccgatatc gctcctgacg agttctgtac aggtgaagag
      721 atagacagtt ccagaaaccg cagacctgca tagccgaccg ccatcgataa aatgtacaac
      781 gccgatactg gaagataagt aatcatgttg cgcggttcgt agaccacgcc cgaagtcagc
      841 aggacataca tagcatgaat acccatgacc gctccaacca ttatgacaat cttcaggtaa
      901 ctgtatttgt ctttcggatc gcttcctttt ttataaaata aatccgctgt accccaagcc
      961 aacacggaca caatcgcaaa taaaaaccac atgctccatc catcctttat aaaataattt
     1021 caaaaactaa cttactaatt atactagacg gatggtgttt gcaataacaa taacgcaatg
     1081 ttaacaacat atttacagat ttatcgtaag tcacacgcaa tataatcgtt ggaaaaaatc
     1141 agtcgcggac tcttttgccc atgtatacga gatcgcgctc gatgccgtcg agttcggcca
     1201 ctctcggcaa gtagccccat tgttcgaagc caaatttttt catcagcccg atgctcggtg
     1261 cattgtggcc gaagatgaga caaacaagat tcgtgatatc caactcgtcg caagcagcca
     1321 atactgtttc cataatctgc gtgcccattt ttttaccgcg gtagccttca tggatataaa
     1381 tgctcaattc tgctgtctta tcgtaggcgg cacgctcgtg gaaagcctga aagctcaccc
     1441 aagcacagat tttcccatcc tcttcaaaaa cccagatcgg tctgtagctg ggcgaatggt
     1501 ccaggaacca gcccattttt tgtttgacgg tcaccggttc cagatcggcc gtcaccatcc
     1561 ggctggggat tgtctgattg tagatagcga cgatgtcttc cacatcgtcc aaagttgcat
     1621 ttctatgtgt aagggtcatt tttattcctc caaaaatcaa aaattcacag cccaacttct
     1681 tgaaggacta tacgctattt taccagtttg gccaggggaa atgccatcta ttttcaaaag
     1741 agagcacaaa tactattttc ccttcccaat ttctgcagga ttgccgcagt ttttcgtaat
     1801 cttcccaatg aaaatcggtt atactggata caagaatggg gtggacaaca ttatgaatga
     1861 agaaatatat atcgcagaag atgaagatgg catccgtttg acggtgaaga cgtttctgga
     1921 gagcgagggc tatgcggtct ccgactttcc caatggcgac ttgctttacg atgctttcct
     1981 ggataaacaa ccggacctgg tcatcctgga tgtgatgatg ccgggatcta gtggtttcga
     2041 aatcaccaaa aaaatacgcg acatcagttc ggtgccgatc atcatcctga ccgcaaaaga
     2101 cagcgacatg gattacgcga cgggcatcaa cttgggcagt gatgattact tcacgaagcc
     2161 cttcagtgcc atcgccctga atatgcgcgt aaaagcgatg cttcgccgca tcaagatgga
     2221 tcaggaacgc ctgccggccc gtcaggaaga agcgacgctg cggatcggtg atatcgaaat
     2281 cgatctggcc gccatgtccg tcaaacaaaa tggggtctcc ctcaacctga caccaaatga
     2341 atacaatgtt ttcgtttatc taatcgaaaa caaaaaccgt gccgtttcca gggacgagct
     2401 tttggattcg atctggggat acggcaagga catcgagacg cgggcctgcg atgatacagt
     2461 cagacggctg cgcaaaaaac ttgccggctc cacagtcgag atcgagaccg tttggggatt
     2521 cggcttcacc gtgaaggagt cgtctgcatg atgaagatga agacatcgaa aaaaagtttg
     2581 aagatccggc ttctgacgac cgtcaccttg ttgttggcgg ccatctttac cttgatttat
     2641 ctggtgttca attttttctt tacccagtac atcgaaacag ccgcgacctc gctgctttcc
     2701 gaagcgagac aagattacat cgagcgtc
//
LOCUS       DAOMUJ010000238         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10691_length_2726_cov_1.947211, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000238 DAOMUJ010000000
VERSION     DAOMUJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10691_length_2726_cov_1.947211"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLT73_06430"
     CDS             complement(<1..124)
                     /locus_tag="PLT73_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM20050.1"
                     /translation="MPNFKIVTDSTTELSAEEIDRYGITVIPLSSMIENVIYYDG"
     gene            298..>837
                     /gene="truB"
                     /locus_tag="PLT73_06435"
     CDS             298..>837
                     /gene="truB"
                     /locus_tag="PLT73_06435"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM20051.1"
                     /translation="MDGILPLWKERGMTSHDCVFKLRKILKTKKVGHTGTLDPEVDGV
                     LPICIGKATKVVEFLTDTDKAYEGEITIGVATTTEDSQGEPIAKTPVDRTLSLAEIDA
                     AMEAMVGESIQVPPMYSAVKVNGKRLYEYARKGLTVERPQRTIQVMHFERISEPVYHE
                     EDETMSWCFRVTCGKGTYVR"
     assembly_gap    838..847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <848..1191
                     /gene="truB"
                     /locus_tag="PLT73_06440"
     CDS             <848..1191
                     /gene="truB"
                     /locus_tag="PLT73_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124672.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM20052.1"
                     /translation="GYQAYMSSLTRTMSGGFTQTDCLTLQQVAEAMANDVIDENLKPI
                     DSVFASYRQVALDDELWDKVRNGAVLPNVELFEVMDGPVLFTYQSKIVAMYEPHPTKA
                     GYIKPRKMFLS"
     gene            1214..2176
                     /gene="ribF"
                     /locus_tag="PLT73_06445"
     CDS             1214..2176
                     /gene="ribF"
                     /locus_tag="PLT73_06445"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRM20053.1"
                     /translation="MEIIHMHHPYDQKQIPDEQIVLALGYFDGVHRGHQEVIKRAKEI
                     AENKNLKLAVMSFNHHPSIVFQKMNPETMQYLSTINRKAEIFENLGVDYFFVVSFTSA
                     FASLRPQEFVDQYICGLHASAVVAGFDYTYGPREIADMKQLVHYAKDRFEVIEVAELK
                     NEVEKISSTHIREALTEGDMEKANAYLGYVYQIEGTVIHGDARGRLLGFPTANIQTEK
                     HTRLPRNGVYIVSIRVNGTWHRGTASIGHNITFEAGRDKTVEVYILDFDKMIYGENVA
                     VRWHHFIRSEIKFSGIEQLIAQLKSDEADTIAYFQDYPLDEVTL"
     gene            2177..>2726
                     /gene="hemW"
                     /locus_tag="PLT73_06450"
     CDS             2177..>2726
                     /gene="hemW"
                     /locus_tag="PLT73_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016379472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRM20054.1"
                     /translation="MAAAYLHIPFCEHICFYCDFNKVFLEGQPVDEYVDALIKEMQLS
                     KQLHPEETLSTFYIGGGTPTTLNERQLEKLLNGIRSTYSLPKSAEFTMEANPESVSFE
                     KLKIMRDYGVNRLSMGVQSFNNDILKKIGRIHTAEQVYTSVADARKAGFDNMTIDLIF
                     RLPNQTMADFEDSLKKALELDLP"
BASE COUNT          803 a          565 c          673 g          675 t
ORIGIN      
        1 ttccgtcgta ataaatcaca ttctcaatca tggacgaaag cgggattacc gtgattccat
       61 atctgtcaat ttcttctgcg gacaattccg ttgtggaatc ggtcacaatt ttgaagtttg
      121 gcatattgat tctccttaag tacagtcata tttccttaag catagcagat tttagaggaa
      181 aagtaacgca gttatcctta tgatttgcag gcatatttct ctaatcttga tgttttttcc
      241 tgtagaatag attatgctat aataggttag ttgcagaaga aagtgggtta caaatatatg
      301 gatggcatat tacctttatg gaaagaacgc ggtatgacaa gtcacgactg cgtattcaaa
      361 ttgagaaaaa tactgaaaac aaaaaaagtt gggcacaccg gaactttaga tccggaagtt
      421 gatggcgttt tgccgatctg catcgggaaa gccacgaaag tcgtcgagtt tttgacagat
      481 acggataaag cgtacgaagg tgaaatcact atcggagtcg ctacgacgac ggaagatagc
      541 caaggtgaac cgatcgcaaa gacacctgtc gatcgaacgc tgtcattggc tgaaatcgac
      601 gcagccatgg aggcaatggt aggtgaaagc attcaagttc caccgatgta ctccgcagtc
      661 aaggtgaacg gcaagaggtt gtatgaatat gcccgtaaag gattgaccgt cgaaaggccg
      721 caaaggacta tccaagtgat gcattttgaa cggatttcgg aacctgtcta tcatgaggaa
      781 gacgaaacga tgtcctggtg tttccgggtc acttgcggca aaggaaccta tgtgaggnnn
      841 nnnnnnntgg gttatcaggc ttacatgtca agtttgaccc gcacgatgag cggaggcttc
      901 acgcagactg attgcctgac gttgcagcaa gtggctgaag caatggcgaa tgacgtgatt
      961 gacgagaacc tgaaaccgat tgattcggta ttcgcatcat atcggcaggt agcacttgat
     1021 gatgagctgt gggataaagt gagaaatggc gcggttctgc cgaatgtgga acttttcgaa
     1081 gtgatggatg gcccggttct gttcacctat cagagcaaaa ttgtggcgat gtacgaaccg
     1141 catccgacta aagccggtta cataaaaccc agaaaaatgt tcctatcata atcagcaagg
     1201 agttgctaac gcaatggaaa taatacatat gcaccatccc tatgaccaaa aacaaatacc
     1261 agacgaacaa atcgtgctgg ctttggggta ttttgacggc gtccatagag ggcatcaaga
     1321 agtaatcaag agagcaaagg aaattgccga aaataagaat ttgaaattag ccgtcatgag
     1381 cttcaaccat cacccgagca tcgtgttcca gaagatgaat ccagaaacga tgcagtatct
     1441 gtcgaccatc aaccggaagg ccgaaatatt tgagaacctg ggtgttgatt atttttttgt
     1501 cgtctctttc acatctgcat ttgcctcatt aagaccacaa gaattcgtcg accagtacat
     1561 ttgcggcttg catgctagcg ctgttgtcgc cggtttcgat tatacttatg gtcctagaga
     1621 aatcgcggat atgaaacaat tggtgcatta tgctaaagac cgctttgaag tcatcgaggt
     1681 cgccgaattg aagaacgaag ttgaaaaaat cagttccacg catatccggg aagcattaac
     1741 cgaaggggat atggaaaaag cgaatgcata tttaggctat gtctaccaga tcgaaggcac
     1801 ggtcattcat ggtgatgcta gaggcagact actgggtttc ccgactgcga atattcagac
     1861 ggaaaagcac acgcgcttgc ctaggaatgg cgtttatatc gtgagcatca gggtgaatgg
     1921 aacgtggcat cgcggcacgg cttcaatcgg gcacaatatc acttttgaag caggccgcga
     1981 taagactgtt gaagtgtaca tattggactt cgataaaatg atttacggtg agaacgtagc
     2041 tgtcagatgg caccatttta tccgcagtga gatcaaattt tcgggcatcg agcaactgat
     2101 tgcgcagctg aaaagcgacg aagcggatac aatcgcctat tttcaggact accctttgga
     2161 tgaggtgaca ctctaaatgg cagccgctta tttacacatc ccattctgcg agcacatctg
     2221 cttttattgt gatttcaaca aagtgtttct ggaaggccaa ccggtcgatg aatatgtgga
     2281 tgctttgata aaggaaatgc agttatctaa gcagctgcat cctgaagaaa cgctaagcac
     2341 cttctatatc ggcgggggta cgccgaccac gcttaatgaa cgacagttgg aaaagctgtt
     2401 gaacggtatc cgcagcacct attccctgcc taaaagcgcc gaattcacga tggaagccaa
     2461 tccggagagc gtttcattcg aaaaattgaa gatcatgcgg gactacggtg tcaaccgact
     2521 gagcatgggg gtccaatcct tcaataacga catcttaaaa aaaatcggcc gcatccatac
     2581 agccgagcag gtgtacactt ccgtggccga tgcacgcaaa gccggatttg ataacatgac
     2641 catcgatctg attttccggt tgccgaacca aacgatggct gattttgaag acagcctgaa
     2701 aaaagcactg gaattggatt tgccgc
//
LOCUS       DAOMUJ010000239         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10705_length_2723_cov_2.682909, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000239 DAOMUJ010000000
VERSION     DAOMUJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10705_length_2723_cov_2.682909"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PLT73_06455"
     CDS             <1..1846
                     /locus_tag="PLT73_06455"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM20055.1"
                     /translation="SYANKLIDWRFAYDPFEVVSKFYIGAFNFDQMPSGHPNLFIGTV
                     ALTAYIYYFFHPGFSKKERFTSLALTALFFLAMNVQFLNKIWHAGQYPIWYPYRFSFT
                     VCFFFLLNGFRALRTYRPFPFSLACCLLIANAATAIYVLKKDFEFLEPVQVLISAVVS
                     VLVLVLLMLRDQPRKWLTYTLLFVVTAEMTANAAIDLFRLGYVKQDEFSDYQTNLNTM
                     LADVRTPEDTFYRIEKTFQRSKNDSFQANYAGINHFSSTFEKEIPALFGSLGFPDGNG
                     FIVYSTGTLFTDALFGVKYFIQDKKLPETFYDLNDGVYRLKRNSTRPDLTQYSPYSET
                     DRTTTFENPYALSLAFGVSESVLGLELIEDQPLLMQEQLLDAFNNQESIEPYFSLRPF
                     DSNVFQNVTSAADDAQNTTYYRSVDGAVSRIEFQFTPTSGNPYYLILDAGIDDDNANL
                     YVNNVKLDYYTTYRNDQIINIASNQQGENVTFTIELLEDSIRVQDLKLYELNKALFEE
                     TISARQAEGMTITSFSQTRITGSVVIEAEDEILLTTIPYSEGWEVTIDGTTAETTTAL
                     NSLLAIPISPGKHTVVFAYRTPYLMTGLSISVISIAGALLLRKYRKNK"
     gene            complement(2272..2484)
                     /locus_tag="PLT73_06460"
     CDS             complement(2272..2484)
                     /locus_tag="PLT73_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRM20056.1"
                     /translation="MQIGLQQKQMTGEAITDDDVASAQAIAERASGNEVIQSLMAKEQ
                     QLSQLMEEINAIITKPLQELYAPAQD"
BASE COUNT          724 a          658 c          583 g          758 t
ORIGIN      
        1 aagctatgcc aacaagctga tcgattggcg tttcgcctat gatcccttcg aagtcgtttc
       61 gaagttttat atcggagcgt tcaattttga ccaaatgccc agcggacacc ccaatctttt
      121 tatcggaact gtcgcgctga cagcctacat ttattatttt tttcatcccg gtttctcaaa
      181 aaaagaacgg ttcacctcgc tggcgttaac cgccctcttt tttctggcga tgaacgtcca
      241 gtttctgaat aaaatttggc atgccggaca atatccgatc tggtaccctt accgtttttc
      301 gttcaccgta tgtttctttt ttctgctgaa cggttttcgg gcgttgcgta cttatcgtcc
      361 ttttcccttt tcgctggcct gctgtctttt gatcgcgaat gcggccaccg ccatctatgt
      421 gctgaaaaag gattttgaat ttttggagcc tgtccaagta ttgatcagtg cagtcgtttc
      481 tgtccttgtc ctagtgttgc tgatgctcag ggatcaaccg agaaaatggc tgacctacac
      541 attgcttttc gtggtgaccg cagagatgac ggcaaatgca gccatcgacc tgttccggtt
      601 gggctacgtc aaacaggacg aattctctga ttatcaaacg aatctgaata cgatgctggc
      661 ggatgtgcgc acacccgaag acacattcta ccggatcgaa aaaacgttcc aacgttccaa
      721 aaacgacagc ttccaagcca attatgcagg aatcaatcat ttcagctcca cttttgaaaa
      781 agagattccc gccttgttcg gatcattggg ctttcccgac ggcaacgggt ttatcgtata
      841 ctcaaccggg acattgttta cggatgcgct tttcggtgtg aaatacttca tccaggataa
      901 gaagttgccg gaaacatttt atgacctcaa tgacggcgtg taccggttga aacgcaactc
      961 cacccgcccg gatttgaccc aatacagtcc ttattccgag accgatcgaa ccaccacttt
     1021 tgagaatcca tacgcgttgt cactggcatt cggtgtttcc gaaagcgtat tgggtctcga
     1081 gttgatcgag gatcagcccc tattgatgca ggaacaactg ttggacgcct tcaacaatca
     1141 ggaaagtatc gaaccgtatt tttcattgcg cccgttcgat tccaacgttt tccagaatgt
     1201 cacatccgct gccgatgatg cgcagaacac aacctactat cggtctgtgg atggtgcagt
     1261 ctcaagaatc gagttccagt tcacccctac cagcggcaat ccttattatc tgatcctgga
     1321 tgccggcatc gacgatgata acgcaaacct ttatgtgaat aatgtgaagc tggattacta
     1381 tacgacctat cgaaatgatc agatcatcaa catcgcaagc aaccagcagg gggagaacgt
     1441 caccttcacg atcgaactgc tcgaggactc gatacgggta caggacttga aactttacga
     1501 attgaacaaa gctctgttcg aggaaaccat ttcagcgcga caagcggaag gcatgacgat
     1561 cacttcgttc agccagacac gcattacagg ttctgtcgtg atcgaggctg aagatgaaat
     1621 attgttgacg acgatcccct acagcgaagg ttgggaggtc acgatcgatg ggacaactgc
     1681 agaaacaact accgctctga atagcttact ggcaatacca atcagcccgg gcaaacatac
     1741 ggttgtattt gcgtaccgta ccccttatct gatgactggc ttgtccatca gcgtgatttc
     1801 catcgctggt gccttattat tgagaaaata tcgcaaaaat aaataagaaa agctgaacgg
     1861 gttcgttcag ccctgaaaga aatttaggaa atctgtccac gcaacgttcc ctacggtcac
     1921 cttgtactgg ggctctaagg gatgaatccc taagagtccc atgcaactga gcatcgaaga
     1981 gcgcaatagg acagatttat ctaatttccg aagggcttac ccgtgaagct agccatctat
     2041 attggatgca tgaaatattt tgcaatcctg tgaaaaacct acggtgacgc acttttgatt
     2101 ctaaaaattt ttatatgatc ctagaagcat caaaaaagct gcgccatttc ttccaaatag
     2161 cggatgaaga acgttggatg gcattgatcg gcaatgcgtt gaatcctgtc cttgtctatc
     2221 atccggccat tctttcggaa atggtcacag cttgtcgtca aaaattaagt cttaatcttg
     2281 tgcaggcgcg tatagttctt gcaacggttt tgtgatgatg gcattgattt cttccatcaa
     2341 ttggctcaac tgttgctctt tagccatcaa agattggata acttcgttgc cggatgctct
     2401 ttcagcgatg gcctgagcag aagccacatc gtcatcagtg atcgcttcgc cagtcatttg
     2461 tttttgttgc agtcctattt gcacattgat cttctctcat tgcagtataa gctgcagaca
     2521 actccgtaaa aacagaagcc tcacgcagat ttttttccaa ctcataagct gtatcataaa
     2581 tattactcat tatctcattc cttccattcg catattacat aaactataac attatccgaa
     2641 caataaagcc atcaaaaaac acgaaaatac gttcttatca gagcgtgatg aatcccgggt
     2701 agaaatcgag cactaagagg act
//
LOCUS       DAOMUJ010000240         2712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10766_length_2712_cov_2.001129, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000240 DAOMUJ010000000
VERSION     DAOMUJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10766_length_2712_cov_2.001129"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..721
                     /locus_tag="PLT73_06465"
     CDS             227..721
                     /locus_tag="PLT73_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM20057.1"
                     /translation="MNNELKEKILKIIEKNNKLTDEEVAVMLGVETEEVAIAVKELEA
                     DKIICGYHTLINWDKTGDDEVSAIIELRVNPQRGSGFDRIAEKVYQFPEVETVFLMSG
                     GFDFMVQLKKAPMKEIALFVAKRLSVIDEVQSTTTHFVLKRYKDHGTLFVDEGDDKRM
                     VLTP"
     gene            718..>1274
                     /locus_tag="PLT73_06470"
     CDS             718..>1274
                     /locus_tag="PLT73_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM20058.1"
                     /translation="MRNPINAKVREIKPSGIRRFFDIANEIPGVISLGVGEPDFDTPW
                     IVRDEGMYTLQKGRTFYTANAGLAELRQEICRYLERRYDLHYSPEDETVVTVGGSEAI
                     DLALRAMLDPGDEVVYAEPCYVSYLPCIVLADGVPVAIPLQEKNQFRLTKEELEAAIT
                     PKTKVLILSYPNNPTGAVMSKEDLEA"
     assembly_gap    1275..1284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1285..1895
                     /locus_tag="PLT73_06475"
     CDS             <1285..1895
                     /locus_tag="PLT73_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM20059.1"
                     /translation="IAHDLFVITDEIYSELSYNGHHVSIASLPGMKERTVYINGFSKG
                     YAMTGWRLGYATAPPIIMEQMIKIHQFAIMAAPTTSQYAGIVALRNCDDNVAEMRESY
                     NQRRRYLMKILKDLELPCFVPYGAFYIFPNITEFGLSSEEFATRLVLEEKVAVVPGSA
                     FGESGEGFIRISYAYSIEELKEAFEHIKSFIERLRAEKKSNQ"
     gene            2127..2441
                     /locus_tag="PLT73_06480"
     CDS             2127..2441
                     /locus_tag="PLT73_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM20060.1"
                     /translation="MLFNLFNSLPSISTKELEAKLTEKPQIIDVREPNEFIGGHIPGA
                     KNVPLRKMEAYTPKGKVYVICQSGARSKRAATILKSKGYDVVNVKGGMSAWSGPTRGG
                     KL"
     gene            2441..>2712
                     /locus_tag="PLT73_06485"
     CDS             2441..>2712
                     /locus_tag="PLT73_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20061.1"
                     /translation="MKIVIIGGVAGGMSAATRLRRLNEDAEIIVLEKGPFVSFANCGL
                     PYYISGDIQERSDLLVQTPEQLRARFDLDVRPFSEAIGIDADKKEVT"
BASE COUNT          788 a          580 c          648 g          686 t
ORIGIN      
        1 aatccctata tgttaagaaa aaacttcaca agtccgcgct acgcgatctt gtggagtttt
       61 ttgcgttttt gccagtattg atgcaccatc ccgccaatca ccattcccga aaacttattt
      121 gcaagtgctt gtcaggggtg ataaaatgaa agaaatttca tttatcaaaa atgaatacta
      181 gagcctgaaa atggcccgaa tgcgtaaagc cagaggaggc aacacgatga ataacgaatt
      241 gaaggaaaag attctgaaga ttatcgaaaa gaacaacaaa ttgactgatg aagaagtggc
      301 ggtcatgcta ggtgttgaaa cagaagaagt ggcgattgct gtgaaagaac tggaagcgga
      361 caaaatcatc tgcggctacc atactttgat caattgggac aaaacgggcg atgacgaagt
      421 ttctgctatc atcgagttac gcgtaaaccc acaaagaggc tccggcttcg accgtatcgc
      481 tgaaaaagtt taccaatttc cagaagtgga gaccgttttc ctgatgtccg gtggcttcga
      541 ttttatggtg caattgaaga aagctccgat gaaagagatc gcactatttg ttgcgaaacg
      601 cctttctgta atcgacgaag tacaatcaac cactactcat ttcgtattga aacgttataa
      661 agatcacggc acgttgttcg tggatgaggg tgacgacaaa cggatggtgc tgactccatg
      721 agaaatccga taaatgctaa ggtgcgcgaa atcaaacctt ccggcatccg gagatttttt
      781 gatattgcga atgagatacc tggagtgatc tctttaggag taggcgagcc tgactttgat
      841 accccttgga tcgtgcgtga cgaaggcatg tacaccctcc aaaaaggacg caccttctac
      901 acggcaaatg ccgggttggc cgaactgcgc caggaaatct gccgctatct ggaacgccgc
      961 tatgatctgc actattcccc ggaagatgaa actgtcgtta cggtgggcgg aagcgaagcc
     1021 atcgacttgg ctttgcgtgc catgttggac ccgggcgacg aggtcgtcta cgcggagccg
     1081 tgctacgttt cctatctccc ctgcatcgta ttggctgacg gagttcccgt tgccatcccg
     1141 ttgcaggaga agaaccaatt ccgtttgacg aaagaagaac tggaagcagc gatcactccg
     1201 aaaacgaaag tactgattct ctcttatccc aacaatccta ccggggcggt tatgtccaaa
     1261 gaggacttgg aggcnnnnnn nnnntcatcg ctcacgatct tttcgtcatc accgatgaaa
     1321 tctacagtga actatcctac aatggacatc acgtcagcat tgcatcctta ccgggaatga
     1381 aggaacggac tgtgtacatc aacggcttct caaaagggta cgcgatgacc ggttggcgtt
     1441 tgggctacgc aactgctcca ccgattatca tggaacagat gatcaaaatc catcaatttg
     1501 ccattatggc tgcaccgacg acaagccaat acgcgggtat cgttgctttg cggaattgtg
     1561 atgataatgt agcggagatg cgtgaaagct acaatcaacg tcgccgctac ttaatgaaaa
     1621 tcctaaaaga tttggaattg ccatgttttg tgccttatgg tgccttttat atcttcccga
     1681 acataaccga gttcggcttg agctcggaag aattcgctac ccgtttggtt cttgaggaga
     1741 aagtggctgt tgtgcccggc tcggccttcg gagagagcgg agagggcttc atccgtattt
     1801 cctacgctta ttcaatcgaa gagctgaaag aagctttcga acatataaaa agctttatcg
     1861 aacgcctacg tgcagagaaa aaaagcaacc aataagaaga aagactgcat aattgcgttc
     1921 gtacgcaact tttgcggtct tttttctcta tactatgcgg gagagcggtt taggcgttta
     1981 ttttgttgag tatcgaaaga atggccatgt atcaggcttt ttttacattt tcaaaatttt
     2041 tgaataataa ctgtttacaa aatataccct atggggtatt ataggtccat aagcgattga
     2101 tactacttac gaaaaaggag ccaagtatgc tatttaattt attcaacagt ctgccttcaa
     2161 tatccactaa ggaattggaa gctaaattaa ccgagaaacc acaaataatc gatgtccgtg
     2221 aaccgaacga attcatcgga ggacatattc ccggagcaaa aaatgttccg ttgagaaaaa
     2281 tggaggcata tacccccaag ggtaaggtgt acgtaatctg ccaatcaggt gcacgcagca
     2341 aacgtgcagc gactatctta aaatccaaag gctacgatgt agtcaatgtc aaaggcggca
     2401 tgtccgcatg gtcgggccct acaagaggag gaaaactatg atgaaaattg ttattatcgg
     2461 tggagtggca ggcggtatgt ccgctgcaac acgtttgaga agattgaatg aagatgcaga
     2521 aatcatcgta ttggaaaaag gacctttcgt ttcctttgcc aattgcggct taccatacta
     2581 tatttcgggt gatatccaag aacgcagcga tcttttggtt caaactcctg aacaactgcg
     2641 cgcacgtttt gacttggatg tccgtccttt cagcgaagca atcggaattg atgcggataa
     2701 aaaagaagtg ac
//
LOCUS       DAOMUJ010000241         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10829_length_2700_cov_2.855198, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000241 DAOMUJ010000000
VERSION     DAOMUJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10829_length_2700_cov_2.855198"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1818
                     /gene="purH"
                     /locus_tag="PLT73_06490"
     CDS             277..1818
                     /gene="purH"
                     /locus_tag="PLT73_06490"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRM20062.1"
                     /translation="MTRALISVSDKTGVVAFAQALAAKGIEIISTGGTKKALEEAGVP
                     TISVEDVTGFPEMMDGRVKTLHPLIHGGLLGRRDLADHMQAMKEHNIAPIDFVVVNLY
                     PFKETISKNDVTLADAIENIDIGGPSMLRSAAKNYASVTVLTDPIDYEKVLAELEAAG
                     ETTFETRQALAAKVFRHTASYDALIAQYLTNVVGETEPEKLTVTYDLKQSLRYGENGH
                     QTATFYQEPLAVPFSIASAVQLHGKELSYNNIKDADAAIRIAREFDEPAVVAVKHMNP
                     CGVGIADDIDAAFDRCYAADPVSIFGGIVVANRPLDVATAEKLNSMFLEIVLAPSYTE
                     AALAILSKKKNVRIMTLDFSAAKAGGKEFVSVLGGMLEQTQDINTEDVPANWEVMTER
                     QPTEDEMKAMDFAWKVVKHVKSNAIVLANSKQTVGIGAGQMNRVGSVKIAIEQAEAAN
                     AIEGAVLASDAFFPMNDSVEYAAKHGIKAIVQPGGSIKDQDSIEMANKYGITMLKTSV
                     RHFRH"
     gene            2107..>2700
                     /locus_tag="PLT73_06495"
     CDS             2107..>2700
                     /locus_tag="PLT73_06495"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182514.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRM20063.1"
                     /translation="MKLLVIGSGGREHAIAKKLMQSPLVETVYCAKGNPGMAQDGITL
                     VDIAENDHQGLIQFVKDNNIAWTFVGPEIPLFEGVTDAFEAAGLKVFGPSKKAADLEC
                     SKQFAKDLMKKYGIPTAAYETFEDYEAAVAYVEANGVPIVIKADGLAAGKGVVVAMTM
                     TEALDALNDMLLQGKYAAGKPKVVIEEYLEGEEFSLFA"
BASE COUNT          783 a          638 c          694 g          585 t
ORIGIN      
        1 gcgtcagcag gttagtcgaa tagtatcctt ggctcgcaga gacaaggggt tcctttatcc
       61 tttccgaagg gctaacccgt gaagctagcc accattatta ggtgcatgaa acattttgca
      121 aatgtgtgga acacctacgg tgatgcgctt ttatgttgaa aacaatttta taaattccta
      181 tagcctaaca aaatggatga ggactccgcg cttgcggaag tccttccttt tgttaaaacc
      241 gcatgaatcc atcataaatt taaggagtga cacacagtga caagagcatt gatcagtgtt
      301 tcagataaaa caggcgtagt agcgtttgcg caggcattag cagcaaaagg aatcgaaatc
      361 atctcaactg gcggaacgaa gaaagctttg gaagaggctg gcgtaccgac tatttccgta
      421 gaggatgtaa ccggctttcc tgaaatgatg gacggccgcg taaaaacgtt gcacccactg
      481 atccatggcg gacttttggg cagacgtgat cttgcggacc atatgcaagc catgaaagag
      541 cacaatattg caccgatcga tttcgttgtc gtcaacctgt acccattcaa agaaaccatc
      601 agcaaaaacg atgtgacttt ggctgatgcc atcgaaaaca tcgacatcgg cggcccaagc
      661 atgctccgca gcgcggctaa gaactacgcg agcgtaactg ttttgaccga tccgatcgac
      721 tatgaaaaag tccttgccga attggaagct gccggcgaaa cgactttcga aacacgccaa
      781 gctttggctg caaaagtgtt ccgtcataca gccagctacg atgctttgat cgcccaatac
      841 ttgaccaatg tggtcggcga aaccgaacct gaaaaattga cagtgactta tgatctgaaa
      901 caaagcctgc gttacggaga aaacggccac caaacagcga ctttctacca agaaccattg
      961 gctgtaccgt tctcgatcgc gagcgcggtc caattgcacg gaaaagaatt gtcctacaac
     1021 aacatcaaag atgctgatgc agctatccgt atcgcccgtg aattcgacga gcccgctgta
     1081 gttgccgtca agcatatgaa tccatgcggc gtcggtatcg cagacgacat cgatgcagcc
     1141 ttcgatcgtt gctatgcagc tgatcctgtc tccatcttcg gcggcatcgt ggttgcgaac
     1201 cgtcctttgg atgtggcgac tgccgaaaaa ctgaacagca tgttcctgga aatcgttttg
     1261 gcgccaagct atacagaagc ggctctagcc attctgtcca agaagaaaaa tgtccgcatc
     1321 atgactttgg atttctcggc tgcaaaagca ggcggaaaag aattcgtctc cgtattgggc
     1381 ggcatgctgg aacagacgca ggacatcaac acagaagacg taccggctaa ttgggaagtc
     1441 atgaccgaac gtcaaccgac tgaagacgaa atgaaagcaa tggatttcgc ctggaaagtc
     1501 gttaaacatg tgaagagcaa cgccatcgta ttggcaaaca gcaaacaaac agtcggcatc
     1561 ggcgcaggtc agatgaaccg tgtcggatcc gtcaaaattg ccatcgagca agccgaagcg
     1621 gccaacgcta tcgaaggcgc tgtattggcg agtgatgcct tcttcccgat gaacgacagc
     1681 gtagagtatg ctgccaaaca cggcatcaaa gcaatcgttc aaccaggcgg cagtatcaag
     1741 gaccaagatt cgatcgaaat ggcaaacaaa tacggcatca cgatgctgaa aacaagcgta
     1801 agacacttca gacactaaga tgagatgaaa tccgtttaga ccctgagcac taagaggaca
     1861 aacgcaaagc ggacgctttt tgtccgtgtg cgtttgtcaa cttaggcgga ggggtctgga
     1921 tttcagaact cgattaaagc aagatgagat gaatcccttg gtagaaatcg agcactaaga
     1981 aggcaactgc aaagcgggcg ctttttgccc gtgtgcagtt gacagcttag gcggagattt
     2041 ctgggattca gaactcgatt aataggcaat atcaagcttc atataaaagg ttggaggatc
     2101 tcaaagatga aacttcttgt aatcggcagt ggcggacgcg agcatgccat cgctaaaaaa
     2161 ttaatgcaaa gcccgttggt ggagactgtt tactgcgcca aaggcaaccc agggatggcc
     2221 caggacggca tcacgttagt agacattgcg gaaaacgacc accaaggatt gatccaattc
     2281 gtcaaagaca acaacatcgc ttggactttc gtcggaccgg aaatcccttt gtttgaaggg
     2341 gttacggatg catttgaagc ggctggcctg aaagtcttcg gacccagcaa aaaagcggcc
     2401 gacttggaat gctccaagca attcgcaaaa gacctgatga aaaaatacgg catcccgact
     2461 gcagcctatg agacattcga agactatgag gcagctgttg cttatgtgga agccaatggt
     2521 gttccgatcg tcatcaaggc ggacggtctg gcggccggca aaggcgtcgt tgtcgcgatg
     2581 acgatgacgg aggctctgga tgcactgaac gacatgctcc tgcaaggcaa atatgcagcc
     2641 ggcaaaccga aagtcgtcat cgaagagtac cttgaagggg aagaattctc gttgttcgcg
//
LOCUS       DAOMUJ010000242         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10849_length_2696_cov_1.664900, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000242 DAOMUJ010000000
VERSION     DAOMUJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10849_length_2696_cov_1.664900"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..442)
                     /locus_tag="PLT73_06500"
     CDS             complement(230..442)
                     /locus_tag="PLT73_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRM20064.1"
                     /translation="MEFGKVKWFNNDKGYGFIEFDQQDDDIFVHFTGIAGEGFKRLEE
                     GQRVQFDIAEGVRGPQATNVIVVAES"
     gene            complement(798..1526)
                     /locus_tag="PLT73_06505"
     CDS             complement(798..1526)
                     /locus_tag="PLT73_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20065.1"
                     /translation="MGDKIIFPLNYEGYLKKGLLAFEEGNLQLAEEWIGKAVAIKKTD
                     DMLPLYLMLLQETDQTEKCLEMITEMRPDIASAISVDDIDFLYLKGLLHSGSFDMARE
                     QIEARKNTFGLSMEHQFALEQLEAEVEKAELKNLEKKLKRSSEILQAEKNIDTQPFHK
                     QQTYAQQLKELEDDQFGKIAQRLLMNNNLHRMLKTEIIHQFLARGLHPTVAVRLDGSE
                     EKLDLAGLVPLIESKTYKEGMAYP"
     gene            complement(1644..2426)
                     /gene="accA"
                     /locus_tag="PLT73_06510"
     CDS             complement(1644..2426)
                     /gene="accA"
                     /locus_tag="PLT73_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HRM20066.1"
                     /translation="MKDVKEIITSARSISRLTATEMIHAICEGFVEFHGDRKFGDDPA
                     IVGGIALLDDMPVTVIGTQKGHDVTENVYRNFGSPHPEGYRKAIRLMKQAEKFNRPIV
                     TFINTSGAFCDVDSEDRGIGEAIAESLLVMSQLTVPFIAIFIGEGGSGGALALAMGNE
                     VWMLENTMYSVLSPEGFASILWKDSSRSDEAAKVMKLTPDDLFSLDVIDKIIMETRRK
                     VARKSDDVMLELKQELSAKLKELKQLTPAELVEQRQKRFRNY"
     gene            complement(2423..>2696)
                     /locus_tag="PLT73_06515"
     CDS             complement(2423..>2696)
                     /locus_tag="PLT73_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HRM20067.1"
                     /translation="LTDPTTGGVTASFAMQGDIILAETGATVGFAGKRVIEQTIKAKL
                     PEGFQTAEKVLENGFIDKIVARKDLRRALQHLLLLHAPAEKAGEVQ"
BASE COUNT          667 a          703 c          536 g          790 t
ORIGIN      
        1 aagcgcttcg aattcagccc gatgattatc catcctgccc tctaaaggca ggcctatttg
       61 ctcttgtaca ccgtcctcaa cgatcaaaat cccaatggca gcataccctt cttttccggt
      121 ggccgcccca tccacaaata attttatcaa atgttccaca tcccaaaaaa caaaggtgaa
      181 agaaaatcct gaacggattc tcctcacctt tgcggtttac tgtacttgtt tatgattctg
      241 ccacgacgat cacattagtg gcttgtggtc ctcttacgcc ttcagcgata tcaaactgaa
      301 cgcgttgccc ttcttcaagt cttttgaagc cttctccagc tataccggta aaatgtacga
      361 agatgtcgtc atcctgttgg tcgaattcaa taaaaccata ccctttatca ttattgaacc
      421 actttacttt gccaaattcc attgtacatc ctccttaaac actcaattct ttggaactgc
      481 tttcacaaag acgggttacc cccattatag agaggaatcc ctatagagtc aattattctg
      541 atagcttcat tggcacttat tcaaaaaaac caaaaaattc ggccaactta ttttccaatt
      601 gatcaatttc atcgcggatt ttattttcgc tcggctcgcc cgtcgatccg atcccattgt
      661 aaatgcggta gaccacctcc aaccactccg aaacggacgg tatggtcctg gcttcgaacg
      721 gataaagaag ggacaaatgc atgaaaaagg cattttccat ggcatctgcc acagtcggat
      781 ttttattggc gatttcttca cggatacgcc attccttctt tgtacgtctt tgactcgatc
      841 aaggggacca atccagccag gtccaatttt tcttcgcttc cgtccaacct gacagctacc
      901 gtcggatgga gcccacgcgc aagaaattga tgaatgatct ctgttttgag catccgatgg
      961 agattattat tcatgagtaa gcgttgcgct atcttgccga attggtcatc ctccaattcc
     1021 ttcaattgtt gcgcgtatgt ctgctgcttg tggaacggtt gcgtatcgat gtttttttca
     1081 gcttggagga tttctgaact tctttttagt tttttttcca aattcttcag ttcagctttc
     1141 tcgacttcag cctccaattg ctccaaagca aattgatgtt ccatcgaaag cccgaaagta
     1201 tttttgcgtg cttcgatttg ttcacgagcc atgtcgaatg atccggaatg caataagcct
     1261 ttcaagtaaa ggaaatcaat gtcgtcgacc gaaatggcgg aagcaatatc cggacgcatt
     1321 tcggttatca tctccaggca cttctctgtt tgatcagtct cttgcaacaa catgaggtac
     1381 agcgggagca tatcgtccgt ctttttgatg gcaacagcct tgccgatcca ttcttccgca
     1441 agctgcagat tcccctcttc aaatgccaat agtccctttt tcaagtatcc ttcgtaattt
     1501 aatggaaaaa taattttatc tcccatttcc atcaccccaa tctacgctga ttttcctgtt
     1561 aggtcattat aacacaaaag cgaacgcgaa aaaaaccgcc cacacaactg atgctgtgcg
     1621 tgcggtttat gacagttctt gaatcaataa ttccggaatc gtttctggcg ttgctcgacc
     1681 aattcagctg gagtcagttg cttcagctct ttcagcttgg cacttaattc ctgcttcagt
     1741 tccagcatga catcatccga tttacgggcc actttcctac gggtttccat aatgattttg
     1801 tcgatgacgt ccaatgaaaa gagatcatca ggcgtcaact tcattacttt ggctgcttca
     1861 tcggatcgtg aagaatcttt ccacaaaatg gaagcaaagc cttccggtga caggacagaa
     1921 tacatggtat tctccaacat ccatacctcg ttgcccattg ccagcgccaa ggctccgccg
     1981 ctgcctccct caccgataaa gatggcgatg aaaggaaccg tcaattggct catcaccaaa
     2041 agcgactcag cgatggcttc accgatgcct cgatcttcag aatcgacatc gcaaaaagcg
     2101 cctgaggtat tgatgaaagt aacgatcgga cgattgaatt tctccgcctg tttcatcaaa
     2161 cggatcgctt tgcggtagcc ctccggatgc ggggatccga aattccggta gacattttca
     2221 gtcacgtcat ggcctttttg ggttccgatg acggttaccg gcatgtcatc caacaatgcg
     2281 atgccaccga cgatagcagg gtcgtcaccg aactttctgt cgccgtggaa ttcaacaaag
     2341 ccttcacaaa tggcatggat catttctgtc gccgtcagac gggagatgct tcttgcagag
     2401 gtgataattt ctttgacatc cttcactgca cttcacccgc cttttctgca ggagcatgca
     2461 acagcaataa atgctgcaat gctctcctta aatcttttct ggctactatt ttgtcgataa
     2521 agccgttttc cagcactttt tcagctgtct ggaagccttc tggtaatttt gctttgattg
     2581 tctgttcgat gacgcgcttg ccggcgaaac cgacagtcgc ccctgtttca gctaggataa
     2641 tatccccctg cattgcaaag ctggccgtta cgcccccagt tgtcggatcg gtcagg
//
LOCUS       DAOMUJ010000243         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10895_length_2686_cov_2.008362, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000243 DAOMUJ010000000
VERSION     DAOMUJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10895_length_2686_cov_2.008362"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLT73_06520"
     CDS             complement(<1..917)
                     /locus_tag="PLT73_06520"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRM20068.1"
                     /translation="MNKVHYEALNETVYTEILDNGLQVVLIPKNKYSKTYGIFTTDFG
                     SIDNQFIPINGDKEIKIPDGIAHFLEHKLFEGEVHDAFEDFAKYGASANAFTSFTRTS
                     YLFSATDNITENTNSLLDFVQDPHFTAEGTEKEKGIIAQEIRMYEDNPGWQLFYGLLR
                     NLYPEHPLSVDIAGTVDSIQAITPELLQICYDTFYHPSNMNILMIGNFSPEEMMDTIK
                     QNQAGKTFLPAKPIQRTLPPASVQDIIAYKEIEMDVQRPKVMLGVKGLDADVTGNQAE
                     KYKIYGSLLLELLFGRSSSHFIELYDSGL"
     gene            complement(910..2178)
                     /locus_tag="PLT73_06525"
     CDS             complement(910..2178)
                     /locus_tag="PLT73_06525"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRM20069.1"
                     /translation="MEYKLKQGVTIYIEPSDKYKTVLIQYKFRTIFSGKTATERSLVK
                     NMLETNSKQYPSQNEMDLKMASLYGATLHTQSQRFGKQHVVTMSLSVVNDKFIGGDDT
                     LLEEAFAFLEEIIYGPNVKDGKFHEKTFLREKENLNNYFESLIEDKSAYARMRLNQEL
                     FAGTDQAYQGIGDASFLEDITAASLFECYEEMIGSNQLDILVSGDVAPERVLNILAGQ
                     NFSDRKEIEKEVFCFREKNTEPVISSEAQDINQGKLFFGFSSPVYYMNDHYFAGLVFD
                     GLFGGFPHSKLFQNVREKESLAYSASSGLDFLRGIMTVGTGIEFDKREQVEEIVLAQL
                     KDMQAGDFSDALIAQTKSMLINHYKQNDDYQGRSLAKQYQDILIIENPLSQEQWIAGL
                     NAVTKEQIVAVADKMTLQAIFFLKGEAENE"
     gene            complement(2334..>2686)
                     /locus_tag="PLT73_06530"
     CDS             complement(2334..>2686)
                     /locus_tag="PLT73_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20070.1"
                     /translation="YAGVLISGFLGGVGGAIVSQSISLNFSGSTIAGQGFIAMAAMIF
                     GKWNPIGVMGAAIFFGFAQSLGVIGSYIPVIQNIPSVYLQIAPYVITIIVLVGFIGKS
                     RGPAANGTTYIKSK"
BASE COUNT          713 a          648 c          562 g          763 t
ORIGIN      
        1 atcaaaccgc tgtcgtagag ctcaataaaa tgcgaggaac tccggccgaa aagcaattcc
       61 aacaacagtg aaccatagat tttgtatttt tcggcttggt taccggtaac atctgcatcc
      121 aagcctttca ccccaagcat cactttcgga cgttgcacat ccatttcgat ttctttgtac
      181 gcaatgatgt cctggaccga ggccggcggc aaagtgcgct ggatcggttt ggccggaaga
      241 aacgtcttgc cagcttggtt ttgtttgatc gtatccatca tttcttcggg agagaaatta
      301 ccgatcatca atatgttcat attggatgga tggtagaacg tgtcataaca gatttggagc
      361 aattccggcg taattgcttg gatgctgtcc accgttccgg ctatatcgac ggaaagcgga
      421 tgttcgggat acagattacg gagcagaccg taaaaaagtt gccatcccgg attatcctca
      481 tacatgcgga tttcctgggc gatgatccct ttttcctttt cagtgccttc tgccgtgaaa
      541 tgcgggtctt gcacgaaatc aagcaggctg ttcgtatttt ctgtgatatt atccgtggcc
      601 gaaaagagat aacttgtccg cgtgaatgaa gtgaaagcat tcgctgaagc gccatactta
      661 gcgaagtctt caaaagcatc atgtacttca ccttcgaaca gtttatgctc aaggaaatgt
      721 gcgataccgt ccggaatttt gatttcctta tctccgttta tcggtataaa ctgattatcg
      781 atcgagccaa aatcggttgt gaagatacca taagtttttg aatatttatt ttttggaatc
      841 agaaccacct gtaatccatt atccaaaatt tccgtataga ctgtttcgtt aagagcctcg
      901 tagtgtactt tattcatttt cagcctcgcc tttcaagaaa aatatcgctt gcaaagtcat
      961 tttatccgca acagcaacga tctgttcctt cgtcaccgca ttcaaaccgg caatccattg
     1021 ctcctgcgaa agcgggtttt cgatgatgag gatatcctgg tactgtttcg ccaagcttct
     1081 gccctgataa tcatcatttt gtttgtagtg gttgatcagc atgcttttcg tttgggcgat
     1141 caatgcatcc gagaaatcgc ccgcttgcat atccttcaat tgtgccaaaa caatttcttc
     1201 aacttgctct cgtttatcga actcaatacc ggtaccgaca gtcatgatcc cacgcagaaa
     1261 atccaagccg cttgaagccg agtaagctaa actttctttt tcccggacat tctggaacag
     1321 tttggaatgc ggaaatcccc caaacaagcc atcaaaaacc agaccggcaa aataatgatc
     1381 attcatgtaa taaacaggtg aagagaagcc gaaaaacagc ttgccctgat tgatatcctg
     1441 ggcttcgctc gaaatgacgg gttccgtgtt cttttcgcgg aaacagaaca cctctttttc
     1501 gatctctttt ctgtcggaaa aattctgtcc agccagtata ttcaataccc gttccggcgc
     1561 gacgtctcca gaaacaagga tatccagttg attcgatcca atcatctctt cgtagcattc
     1621 aaacagactg gccgcagtga tatcctcaag gaaactggca tcaccaatac cttgataggc
     1681 ttgatccgtc cctgcgaaca gctcttggtt caagcgcata cgcgcataag ctgacttgtc
     1741 ttcgatcaga gactcgaaat aattattcag gttttccttt tccctcagaa aagtcttctc
     1801 atggaacttg ccatccttga cattgggtcc atagatgatt tcctccagaa aagcgaacgc
     1861 ctcttccaaa agtgtatcgt cgcccccgat gaatttgtca ttgacgacgg acaggctcat
     1921 cgtcacgaca tgctgcttgc cgaagcgctg tgattgtgta tgcagcgtcg ccccataaag
     1981 gctggccatt ttcaaatcca tctcattttg acttggatac tgtttactgt tcgtttccag
     2041 catattcttc accaaagaac gttctgtcgc agtcttacca ctgaagatcg tcctgaattt
     2101 gtattggatc aatacggttt tgtatttgtc ggaaggctcg atgtagatcg taacgccttg
     2161 ctttaattta tattccatcc atttcacgtc ctgtaagcta ttttatttgt cgatagggaa
     2221 aactatactc taattcagac tttttttcaa ttaagctagc cttgatcaac tccccggatt
     2281 ggcgcaaaaa gaaaaggctg gctcgatgag ccaacccttc tcactttcag agattacttt
     2341 gatttgatgt aagtcgtacc attagcggcc gggcctcttg atttgccgat aaaacctaca
     2401 agcacaatga tcgtgatgac gtaaggtgca atctggagat agacggaagg aatattctgg
     2461 atgaccggaa tataactgcc gatgactccc aaactttggg caaaaccaaa gaaaatcgct
     2521 gcgcccatca ctccgatagg attccattta ccgaaaatca tggctgccat agcaatgaat
     2581 ccttgaccgg caattgtgga tccggaaaag ttcaagctga tcgactggga gacaatggcg
     2641 ccgcccacgc cacccaaaaa gccggaaatc aacacgccgg cgtagc
//
LOCUS       DAOMUJ010000244         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10937_length_2678_cov_2.564621, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000244 DAOMUJ010000000
VERSION     DAOMUJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10937_length_2678_cov_2.564621"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLT73_06535"
     CDS             <1..583
                     /locus_tag="PLT73_06535"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016370505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRM20071.1"
                     /translation="EELKEAEIDSILALRGDKNPAVEEKQDFRYASDLVKFIRQYGDF
                     SISGACYPECHTESKNKIEDIAYLKEKVDSGVQHLISQLFFDNNAFYSFQEHIQIAGM
                     HVPVEAGIMPVINKAQIERMVTLCGASLPEKFSRVMHKYEDNKEALFDAGMAYAINQI
                     VDLLAHDVDGIHIYTMNNPIVAKRICDGIKNLI"
     gene            complement(1078..2361)
                     /locus_tag="PLT73_06540"
     CDS             complement(1078..2361)
                     /locus_tag="PLT73_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM20072.1"
                     /translation="MLFKELELNQNLLRALKEAGYTKATPIQEDAIPHLMNNKDLLGC
                     AQTGTGKTAAFALPILQNLTEQETAGKGAIKALILAPTRELALQIGESFQIYAKYLPL
                     KIQVIFGGVSQNPQTTALKRGTDILVATPGRLLDLIGQGYIKLNQVDFFVLDEADMML
                     DMGMLHDVRRIIRELPKKRQSMFFSATMPTEIEKLAGTILSYPVKVEVTPVSSTVEVI
                     HQSVYPVAKTDKTDLLIHLLKEDTVERSLVFSRTKHGANKIVKKLLQAGLSAEAIHGN
                     KSQTARQRALENFKTKKTSVLVATDIAARGIDVPELSHVILFDLPEVPETYVHRIGRT
                     GRAGRGGKAISFCDETEASLLRDIEKLIKRKVPVVTDQPFLLKEGTKAAAPQPKKPIA
                     ARNNGTRVKNQQGPRGGQKAAQRPRFQTKQNTQSK"
BASE COUNT          607 a          647 c          722 g          702 t
ORIGIN      
        1 ggaggaactg aaagaggccg aaatcgatag cattcttgcc ttgcgcgggg ataaaaatcc
       61 ggccgtggaa gaaaagcagg acttccggta cgccagcgat ctggtcaaat tcatccggca
      121 atacggtgat ttttccatca gtggcgcctg ttatccggaa tgccacacgg aatcgaagaa
      181 caaaatcgag gatatcgcct atctgaaaga gaaggtggac agcggggtgc agcatctgat
      241 ttcccaactc tttttcgaca acaatgcctt ctattccttc caggaacaca tccagattgc
      301 aggtatgcac gttccggtcg aggcgggcat catgccggtc atcaataaag cccagatcga
      361 aaggatggtg acgctctgcg gcgcctcctt gccggaaaag ttttccagag tgatgcacaa
      421 atacgaagac aacaaagagg cacttttcga tgccggcatg gcctacgcga tcaatcagat
      481 tgtcgatctg ctggcgcatg atgtggatgg catccacatc tacacgatga acaatccgat
      541 cgtcgcaaaa aggatctgcg acggaatcaa gaatctgatt tgaacgtgaa cttcgtttgg
      601 gcgtcgctta ccggagaagg tggtttcctc cggtgagtgc ccttctcaac ggagttgagg
      661 tccccggtaa tagctgttct cctgcgagcg aatgtttgcc ggagctaagt gcggggacgg
      721 aggctgatct ccgtcgaacg agggctcgac ggaggtgaga gttttgcgca cgcgtcagct
      781 ccggcgaagc caaccctggt cggagaaggt ggtttcctcc ggtgagtgcc cttctcaacg
      841 gagttgaggt ccccggtaat agctgttctc ctgcgagcga atgtttgccg gagctaagtg
      901 cggggacgga ggctgatctc cgtcgaacga gggcttgacg gaggtgagcg ttttgcgcac
      961 gtgccagctc ctgcgaagcc tgcgttcccc ggagcaaaac gtttcatccc gccgcattca
     1021 tcagttttgt cataaaaaga gagctcccgc catttccggc gggagctctc ttgtctgtca
     1081 tttgctttgc gtgttttgtt tagtctggaa acgcggtctt tgcgcggctt tttggccgcc
     1141 tcttggacct tgttggtttt tgacacgagt cccgttattg cgcgcggcaa tcggcttttt
     1201 cggctgtggt gcagctgcct tagtaccttc tttcaacagg aaaggctgat ctgttacgac
     1261 cggaactttc cgcttgataa gtttttcgat gtctcgcaac aacgatgctt cggtttcatc
     1321 acagaatgag atggcttttc cgccgcgtcc tgcgcgtccc gtccgtccga tacggtgaac
     1381 ataggtttcc ggaacttcag gcagatcaaa caagatgaca tgcgaaagct caggcacatc
     1441 gataccacgg gctgcgatat ctgtcgctac aagaacgctg gtttttttgg tcttgaagtt
     1501 ttccaaagct cgttgacgcg cagtctggga tttgttgccg tggattgctt cagctgaaag
     1561 gcccgcctgc aaaagctttt tgacgatttt gttggcgccg tgcttcgttc ttgagaagac
     1621 aagcgagcgt tccactgtat cttccttcaa caggtggata agcaagtcag ttttgtcggt
     1681 cttggctaca ggataaacgc tttggtggat gacttctaca gtcgaggaaa ccggagtgac
     1741 ttcaaccttc accggatatg acaaaatagt tcccgcaagt ttttcaatct cagtcggcat
     1801 agtggccgag aagaacatgc tctgacgttt ttttggcaat tcgcggatga tgcggcgtac
     1861 atcatgcagc atccccatat ccaacatcat atcggcttcg tccaggacaa agaaatcgac
     1921 ttggttcagt ttgatgtaac cttgtccgat taggtccaac aatctgcctg gcgtcgccac
     1981 aaggatatcc gttccgcgtt tcagcgcggt tgtttgagga ttctgggaaa caccgccaaa
     2041 aatgacttgg atcttcaatg gaaggtattt tgcgtaaatt tggaagcttt ccccgatttg
     2101 cagggcaagt tcgcgggtcg gcgcaaggat cagcgctttg atggcgcctt ttccagcggt
     2161 ttcttgttcc gtcagattct gcagaatcgg cagggcaaag gcagcggtct taccggtacc
     2221 ggtttgggcg caacccaaca aatccttgtt gttcatcaga tgcggaatgg catcttcttg
     2281 gatgggcgtc gctttcgtgt agcctgcctc tttcagtgcg cgcagcaggt tttgattcaa
     2341 ttctagttct ttaaataaca aatggttctc cttcagcatg ttctacatgc atttgatttt
     2401 agtgggtatg tttcgggcca ggccagtttg ggaaagagag tcggactctc gtttgccaaa
     2461 ttcctcaccg cgaaacgtta tataggttct ctgagcgaca gcgtgactgg cctactatat
     2521 cacaaatgga agcaaaagtc atagcattcc gcttttgggt ggaataataa gtgggcaaac
     2581 aagacgagga agcgttgagg catccgaaat aacccgcaaa aaaaaccgca ccgaaatgga
     2641 ttcgttcatc catttcggtg cggtcttatt tttgaagt
//
LOCUS       DAOMUJ010000245         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10941_length_2677_cov_1.754005, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000245 DAOMUJ010000000
VERSION     DAOMUJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10941_length_2677_cov_1.754005"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLT73_06545"
     CDS             complement(<1..136)
                     /locus_tag="PLT73_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRM20073.1"
                     /translation="MNYTIAALPGDGIGPEIMESGLTLLETLGKKFQHEFNVTVYPFG
                     G"
     gene            complement(155..1711)
                     /locus_tag="PLT73_06550"
     CDS             complement(155..1711)
                     /locus_tag="PLT73_06550"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM20074.1"
                     /translation="MTEKQYIQFFDTTLRDGEQTPGVNFNTKEKVQIALQMEKWGIDV
                     IEAGFPISSEGDFEAVKAIAGAVKNMTVAGLARCNEKDIDAAYEALKDAADPQIHIFI
                     ATSPVHMEFKLKMSKAEVLASVAHHVAYAKSKFEKVQFSPEDATRSDWDFLVEVVNLA
                     IEKGATVINIPDTVGYTNPTEFGNLFKYLKQNVTRFDEVIFSSHCHDDLGMATANALA
                     AVENGALRVEGTINGIGERAGNTALEEVAVALHIRKNYYEKEMGMVLKETKRTSDLVS
                     RLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPETYEIITPQLVGVEHNSLPLGKLS
                     GRHAFVDRIAQMGYEISDPAEIKILFARFKELADKKKNVTEEDIHALMVGKSIEDESA
                     YELKRLQVQFVKDGIQAAIVGIQDKGNKEAKMQDSATGSGSIEAIYNTINRIMEQEII
                     LKEYNIEAITGGKDAQAEVHVVVEDEDGKSYNGTGIDFDVLTASAKAYIQASGKAKNK
                     QTIEKVSAHF"
     gene            complement(2354..>2677)
                     /locus_tag="PLT73_06555"
     CDS             complement(2354..>2677)
                     /locus_tag="PLT73_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM20075.1"
                     /translation="QKVDAIYIPTDNTLSSTMATVGQIAMEAKIPVIPGATDMVEVGG
                     LATYGIDFKELGRQTGEMALQILEEGKLPSELPVQFPETLQLVINEEMAEALGIDPDS
                     IKMPE"
BASE COUNT          591 a          692 c          576 g          818 t
ORIGIN      
        1 ctcctccgaa tgggtaaact gttacgttga attcatgctg aaattttttg ccgagcgttt
       61 ccagcaaggt caagccgctt tccattattt ctggtccaat gccatctccg ggcaatgctg
      121 caatcgtata attcatctta ctttttcccc ctcatcagaa atgtgctgat actttttcta
      181 ttgtctgttt attttttgct tttccgctgg cttggatgta cgctttcgcg gatgccgtca
      241 gaacgtcgaa gtcgatacct gttccgttat agctcttgcc gtcctcatcc tccacgacca
      301 cgtgcacttc tgcttgcgca tctttcccgc ctgtgatggc ttcgatgttg tattccttca
      361 gaatgatttc ttgctccata atgcggttga tggtattgta gatcgcttcg atgcttcccg
      421 atccggttgc ggaatcctgc atcttcgctt ctttattgcc cttatcctgg atgccgacga
      481 tcgccgcttg gatgccatct ttcacaaact gcacttgcag gcgtttcaac tcgtaagccg
      541 attcatcttc gatcgactta ccgaccatca gcgcatggat gtcctcttcg gtcacgttct
      601 tcttcttgtc agccagttct ttgaagcggg cgaacaatat tttgatttca gcgggatcgc
      661 tgatttcata gcccatttgc gcaatccgat ccacaaaggc gtgacggccg gacagtttgc
      721 caagcggcaa tgagttatgc tccacaccga ccaattgcgg tgtgatgatt tcgtaggttt
      781 ccgggttctt cagcacacca tcctgatgga tcccggactc gtgtgcgtag gcattaccgc
      841 cgatgacggc tttgttgcgc ggaaccggca tgccggaaag gcggctgacc aaatcgcttg
      901 tgcgcttcgt ttccttcagt accataccca tttctttctc ataataattt ttgcggatgt
      961 ggagcgcaac tgcaacttcc tcaagtgccg tatttcctgc acgctctccg atgccgttga
     1021 tggtgccctc tacccgaagc gcgccatttt cgactgcagc caaagcgttc gccgttgcca
     1081 tgcccaaatc gtcgtggcaa tgggaggaga aaatcacctc atcaaagcgg gttacatttt
     1141 gcttcagata tttgaacaga ttaccgaatt cggtaggatt agtgtagcca acggtatcag
     1201 gaatgttgat gaccgttgcg cccttttcga tagccagatt gacgacttca accaagaaat
     1261 cccaatcgct tctggtcgca tcctcagggg agaactgaac tttctcaaat tttgatttgg
     1321 cgtatgccac atggtgtgca acagacgcca acacttctgc tttgctcatc ttcagcttga
     1381 attccatatg gacagggctc gtcgcaataa aaatatggat ttgtggatct gccgcatcct
     1441 tcaaggcttc ataagctgca tcgatgtcct tttcgttgca acgcgcaagt cctgctactg
     1501 tcatgttttt gacagcgccg gcgatcgctt ttacggcctc gaagtcgcct tctgaagaaa
     1561 tcgggaagcc tgcctcgata acatcgatcc cccatttttc catctgcaag gcaatctgta
     1621 ctttttcctt cgtattgaag ttcacaccag gtgtctgttc cccatcgcgc agagtcgtat
     1681 caaagaattg aatgtactgt ttttcggtca tggtcttttc ctccggttca tttttcgttt
     1741 attggtttca ttaaagattc ataagagtaa atttcgaaaa aaaatgcatc cacaaataat
     1801 ggatgcattt cgaaggtcaa taaataatcg cttcagcccc attatttgtc aatgccaagc
     1861 agggctcaaa cgactgatat tccagtgtcc aagccctgct ataataataa gccgttgaaa
     1921 gaaggggtag tccccatgat tacgttagcc gatacgtttg catcttccat gacgatcccc
     1981 tcctttcctt tttttgtagc cttctaattt ttaactaagt tttattatcg tattctcatt
     2041 ttatgcaatg tgccttcttt tgtcaacccc ttttgctgtt tccttacaaa atcgtgtaac
     2101 acatcgctta agccgagggg aaacgcagaa gattgtgtcc acaactccgg tgatggcctt
     2161 ctcaccggag ttcgccggta actttctttc tctcctccga taagtgaggc cttatcggag
     2221 gagacaactc taattgttag accaactccg gcgaggcctc cgtcgccgga gcagaacaat
     2281 atacgctcgg gtacgccaca aaaaaacgaa gctgccacac tttagtgagg cagcttcgtt
     2341 tttaatggat aacttattct ggcattttta tgctgtctgg atcaattccc aatgcttcag
     2401 ccatctcttc attgatgacc aattgcagcg tctcagggaa ttgtaccggc agttcggaag
     2461 gaagtttacc ttcttccaaa atttgcaagg ccatttcgcc tgtctgacgc cctaattcct
     2521 tgaagtcgat tccgtaagtt gccaatccgc ccacttccac catatcagtg gcgccaggaa
     2581 tgacagggat tttggcttcc atcgcgattt ggccaacggt agccattgtg ctggataagg
     2641 tattgtctgt cggaatatag atcgcgtcca ctttttg
//
LOCUS       DAOMUJ010000246         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10946_length_2676_cov_2.097673, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000246 DAOMUJ010000000
VERSION     DAOMUJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10946_length_2676_cov_2.097673"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLT73_06560"
     CDS             complement(<1..1115)
                     /locus_tag="PLT73_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRM20076.1"
                     /translation="MRILHTADWHLGKIVNDFSMLEDQRYYLTNLIELLKDKEIDAII
                     MAGDLYDRALPPKEAVALANRTLTRMVKELGVPVFVIAGNHDSNERIEYAADLLADSR
                     LYIEGTMKDTIRKVAFEGTNFYLLPFADHVYVREKLQDDTIKNMEDAIRAQLATVKAT
                     MNPNEVNILIAHGYVIQTGNDTSEPSDSERPLSIGTSEYVDVSLFEDFDYVALGHLHK
                     AQKVKDDKVRYSGSILKYSKSETPHQKQTSIVTIEKDKLEIEPLRIKPLRDMRTIRST
                     FSELMKGQSEDYLFFELEDTEYVLDAMNQLRRRYPHAMGLEYVSRRETESVALQHNRE
                     DLQQLSYLDLFKDFYEQYRAIEMEESGQKIVADIFYA"
     gene            1305..2090
                     /locus_tag="PLT73_06565"
     CDS             1305..2090
                     /locus_tag="PLT73_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM20077.1"
                     /translation="MDLGLKNKVALVVASSQGLGKAVAKELVKEGAHVMLTSRSEDKL
                     AEVKEELEAMHAGKVAYFPCDITKVEDIQKLVARTHTVFGKIDILLNNAGGPPSGKFD
                     SFSDEVWQQAFALNLLSYIRIIREVLPDLRKEGGRIVNIASISVKTPLPNLVLSNTFR
                     NGIVGLSKTLADELAPDNILVNVVAPGKIETDRLKYLNEANAKARELTVAEVDKAAIA
                     GIPLGRYGTPEEFAKAVVFLLSEANTYITGSTLYVDGGAVKAI"
     gene            2129..>2676
                     /locus_tag="PLT73_06570"
     CDS             2129..>2676
                     /locus_tag="PLT73_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRM20078.1"
                     /translation="MGQVIINADDFGLTNGVNYGIIDSFLYGITTSTTLLANGAAFDH
                     AVALASDHPELEIGVHLNLTLAKPLLPDASTISADGRFHTREYVQQHAASLDLDEVYA
                     EWHAQIEKVRKAGIKPTHLDSHHHVHMLEPLNKVILSLAIQYELPIRDHFEGSHGVLH
                     TDRQFDLGFAAEKPQAELGMSV"
BASE COUNT          669 a          651 c          696 g          660 t
ORIGIN      
        1 aaagcgtaaa agatatccgc aacgattttt tgaccggatt cttccatttc aatggcgcgg
       61 tattgctcat aaaaatcctt gaacaaatcc agataggaca actgttggag atcctcccgg
      121 ttatgctgca aggccaccga ttcggtttct cttcggctga cgtattcgag ccccatcgcg
      181 tgtggatagc gacggcgcag ttggttcatc gcatccagta cgtattccgt atcctccagt
      241 tcgaagaaca ggtagtcctc ggattgcccc ttcatcagtt ctgagaatgt actccggatt
      301 gtgcgcatat cgcgcaaagg cttgatccgg agcggttcga tctccagttt gtctttttct
      361 atcgtcacga tgctcgtctg cttttgatgc ggggtttctg atttggaata cttcaggatt
      421 gagccactat agcggacttt gtcgtctttg actttctggg ccttgtgcag atggcccaga
      481 gcgacataat caaagtcctc aaacagcgac acatccacgt attcggaagt accgatactg
      541 agcggacgtt cggaatcgga tggttcgctc gtgtcgtttc cggtctggat cacatagccg
      601 tgcgcaatca ggatgttgac ttcatttggg ttcatggttg cttttacggt cgccaactgc
      661 gcacgaatgg catcctccat atttttgatt gtgtcgtcct gaagcttctc tcggacatag
      721 acatgatccg caaaaggcag cagatagaaa ttggttccct cgaaagcaac ttttcggatg
      781 gtatctttca tggttccttc tatatacagg cggctgtcgg ccaacagatc ggcggcatat
      841 tcgatccgct cgttgctgtc gtgattgccg gcgatgacga acacaggcac gcccaattcc
      901 ttcaccatcc gggtcagtgt ccgattggcc aacgctaccg cttctttcgg aggcaacgca
      961 cggtcgtaga ggtctccggc cataatgatc gcatcgattt ctttatcttt gagtagctcg
     1021 atcaggttcg tcaagtaata acgctgatct tccagcatgg aaaaatcatt gacgatttta
     1081 ccgagatgcc aatcagctgt atgcagtatt ctcatgggaa aggctcctgt ccattggtta
     1141 gtatttttta agtaaataca ttcattttcc attataccag actacaccgg aagccagcag
     1201 aggcaattat ggccgaccga gtgaaatgct agtgatgtgg acgcttttga gctatagtat
     1261 gtataagagg tcggacgaat taagcagttg aaggaggaac caaaatggat ctggggctaa
     1321 agaataaagt ggcattggtc gttgcttcga gtcaagggtt gggcaaagcg gtggcgaagg
     1381 aactcgtcaa agaaggcgcg cacgttatgc tcaccagcag aagcgaggac aaattggctg
     1441 aggtgaaaga agagctggaa gccatgcatg ccggaaaggt cgcgtatttc ccttgcgaca
     1501 tcactaaagt tgaggacatc cagaaactgg tggcacggac ccatacagta ttcgggaaaa
     1561 tcgatatctt gctgaacaac gcaggcggtc cgccaagcgg gaaattcgat tccttttccg
     1621 atgaagtatg gcagcaggcc ttcgcattga acttgctgag ctacatccgg atcatccgcg
     1681 aagtactgcc tgatttgcgt aaagagggcg gacgcatcgt caacatcgcc tcgatttcgg
     1741 tgaagacacc tttaccgaat ctggtgcttt ccaatacgtt ccgaaacggc atcgtcggcc
     1801 taagcaaaac gctggccgat gaattggcgc ccgacaacat cctcgtgaac gtcgttgcgc
     1861 cgggaaaaat cgaaaccgat cgcttgaaat atctgaatga ggcgaacgct aaagcgagag
     1921 aattgacagt tgcggaagtc gacaaggctg ctatcgcagg cattccttta ggacgttacg
     1981 gtacaccgga agaattcgca aaagctgttg ttttccttct ttccgaagcg aatacataca
     2041 tcaccggcag tacactatat gtggatggcg gggcagtcaa agcgatttga agccgaaccg
     2101 ccatccgaaa cagacaggag aatgaaatat gggacaagta atcatcaacg cagacgattt
     2161 tggtctcaca aatggcgtga actacggcat catcgatagt tttttgtacg gcatcaccac
     2221 ctcgacgacc ctgctggcga acggagcggc attcgatcat gctgtggctt tggcatccga
     2281 tcatccggag ctggaaatcg gagtccatct gaatctgacg ctcgcaaagc ccctattgcc
     2341 tgacgcatcc accatcagtg ccgatgggcg tttccacacc agagaatacg tgcagcagca
     2401 tgcagcttca ctggatctgg atgaggtcta tgcggagtgg catgcgcaaa tcgaaaaagt
     2461 tcgcaaggcg ggcatcaaac caacgcattt ggattcgcac caccatgtgc atatgttgga
     2521 gccgctcaac aaagtcatcc tgtcgttggc gatccagtac gagctgccga tccgggatca
     2581 tttcgagggt agccatgggg tgctgcatac ggaccgccaa ttcgacctcg gcttcgctgc
     2641 tgaaaaaccg caggccgaat tgggaatgag tgtgat
//
LOCUS       DAOMUJ010000247         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10955_length_2674_cov_1.883162, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000247 DAOMUJ010000000
VERSION     DAOMUJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10955_length_2674_cov_1.883162"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLT73_06575"
     CDS             <1..1141
                     /locus_tag="PLT73_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008379060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20079.1"
                     /translation="DESADTAFKEDVYSNMTPVQRVVRTLADILIPLIPALVTTGLLM
                     GLRGLLVELGVEFSPFMLSMFQMLTDTAFAFLPVLIAYSATRKFGGNPIIGIVVGLMM
                     VSPNLPNAWAVAGGSAEAINVSFLGLNIPIVGYQGSIIPAIVAGYLISRIEKELRKVV
                     PQIIDLIVTPFVTLTVTIFLMLFILGPITHTFEIAVSDFIVMLINAPLGIGYILFGAL
                     QQLLVITGLHHTLSIIELQLLADTGENVLNPLMTASMAGQLGAAISAALLMNVKLKRT
                     NAISSSTSTLFGITEPLLFGVNLRSLRILVSGMLGGAVGGLLTYIFGLSATGMGITFI
                     PGLLLYTSSFASMIQYLVVIVGAFVTGFIAVKVQSNKISEQLNLD"
     gene            1759..>2674
                     /locus_tag="PLT73_06580"
     CDS             1759..>2674
                     /locus_tag="PLT73_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20080.1"
                     /translation="MDYKKIAEELLMDIGGKDNVQAATHCATRLRLVLKDESKVDQAG
                     LDARDEVKGTFSTAGQYQIILGSGIVNEVYKEFIKMAGIDAMSKEEVKTAGTQKLNPI
                     QQGVKLLSDIFVPIIPAIVACGLMMGLNNVFTAPDLFVTGKSLIEAYPAIEGFAAMIN
                     TFSNAAYVFLPVLIGFSATQKFGGNPYLGAVLGMLMVHPDLVNAYAYPSVLAAGEVPF
                     WNIFGFEIAKVGYQGTVLPILFASYILAKIVTFLHKRVHTSLDNLVTPLFAIISTGFL
                     TFTLVGPLTRTLGDVMTNSLVWLYDTTGA"
BASE COUNT          733 a          584 c          597 g          760 t
ORIGIN      
        1 ggatgaatca gcggatacag cgtttaaaga agacgtttac tccaacatga ctcccgttca
       61 gagagtcgtt cgaactttgg cggatattct aattcctcta attcctgcac tggttacaac
      121 gggactatta atgggtttaa gaggacttct tgtggagctg ggtgtagaat ttagtccatt
      181 tatgttgagc atgttccaga tgctgacaga taccgcattt gctttcctac cagtacttat
      241 tgcctatagt gccactcgta aatttggtgg aaatcccatc atcggtatcg tagttggcct
      301 tatgatggta tctcctaatt tgcctaatgc ttgggctgta gccggtggaa gcgctgaagc
      361 aatcaacgtt tctttcttag gacttaatat tcctattgta ggttatcaag gatctatcat
      421 ccccgctatc gtagcagggt atctaatatc aagaattgag aaagagctac gaaaagttgt
      481 tcctcaaatc atcgacctga ttgtaacgcc gtttgtaaca ttaacagtaa ccatttttct
      541 aatgctcttt atcttaggtc ctatcactca tacattcgag attgcagtca gtgactttat
      601 cgtcatgctg atcaatgcac ctttaggtat tggatatatc ctttttggcg cacttcaaca
      661 actcttggtt attacaggcc ttcatcacac tttatcaatc attgagcttc agttacttgc
      721 cgatacgggt gaaaatgtgc tgaatccact tatgacagcc agtatggctg gacaattagg
      781 ggcagcaatc agtgcagctt tactaatgaa tgttaaacta aagagaacta acgctatttc
      841 ttcttccaca tctaccttat ttggaattac agaacctctt ctcttcggtg tgaatctgag
      901 aagtttgaga atactggttt ccggcatgtt gggaggagct gtgggtggtc tgcttacgta
      961 tatctttggc ctatctgcta caggtatggg aatcaccttt attcctggac tgttgcttta
     1021 caccagtagt ttcgcatcta tgatccagta cctagttgtc attgtaggag catttgtgac
     1081 agggttcatt gctgtgaaag tacagtcgaa taaaatttca gagcagctta atttagatta
     1141 attaaataaa gaaagagggg agtgcctcag aaccagaaaa tccttttctg gttctgaggc
     1201 actccctttt ctaggtttat gggcagacct accccgccaa accgcaaata agaggctgcc
     1261 tcctattgag acaccccctt ctaacgtgcg cccggaacgg gtgataactt tgtggtgaaa
     1321 gtccatctca ggctttaatg aagaaagcaa caaatcgttg ggtggtactg ataatgtgga
     1381 cccaatgtca aggacattca aaaaaagaga ctttatgcag cactcaataa aacctgctcc
     1441 ctgtaattgt cggaagttcc ttctttttcc ttccacatga ctatcttcag cctcagcgga
     1501 ttacgcggag gccgaatcga gcggaaaggc catcgtcagc cgatctataa aaaatacaaa
     1561 ataaattttt agaaaacgct tgactttatc tttgaaaggg tttactcttc ttattaagag
     1621 gaaccggttt caccaacact gaaaacctgc tccttgtcgt tttgaaccga aaaggaaccg
     1681 gttccgcatg gaagcgaagt attgttatta ttgcacttat tcttaggcag aacagattaa
     1741 agggaagagg gagaaaaaat ggattacaaa aagattgcag aagaattgtt gatggacatc
     1801 ggtggaaaag acaacgtaca ggcggcgacg cactgcgcga cacgactacg cttggttttg
     1861 aaagatgaaa gcaaagtcga tcaagcgggc ttggatgcac gcgacgaagt aaaagggact
     1921 ttctcaacgg ctggtcaata ccagatcatc ctgggatccg ggatcgtcaa tgaagtctac
     1981 aaagaattca tcaaaatggc cggaatcgac gccatgagca aagaagaagt caaaacagcc
     2041 ggcacgcaaa aattgaatcc gatccaacaa ggcgtcaaat tgctttcgga tatcttcgta
     2101 ccaatcatcc cggccatcgt agcttgtggt ttgatgatgg gattgaacaa cgtattcacg
     2161 gcaccggatt tgtttgttac cggaaaatcg ctgattgaag cttatccggc catcgaaggt
     2221 tttgcagcga tgatcaacac tttctccaac gcggcatatg tcttccttcc agtcctgatc
     2281 ggtttcagcg caacgcagaa attcggcgga aatccatact tgggagctgt tttggggatg
     2341 ttgatggtcc acccggatct tgtcaatgcc tacgcctatc catcagtttt ggctgcaggc
     2401 gaagtgcctt tctggaatat cttcggcttc gaaatcgcga aagtcggtta ccaaggaaca
     2461 gtactgccta tcttgttcgc atcttacatc ttggcaaaaa tcgtaacttt cttgcacaaa
     2521 cgcgtgcaca catcattgga taacctggtc acaccgctgt tcgctataat cagcactggc
     2581 ttcctgactt tcactttggt gggacctctg acgcgcacac tcggcgacgt gatgacaaac
     2641 agccttgtat ggttgtatga cacaacaggt gcca
//
LOCUS       DAOMUJ010000248         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_10999_length_2666_cov_2.328993, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000248 DAOMUJ010000000
VERSION     DAOMUJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10999_length_2666_cov_2.328993"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1477
                     /locus_tag="PLT73_06585"
     CDS             188..1477
                     /locus_tag="PLT73_06585"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM20081.1"
                     /translation="MAKKIYIGLLGLGTIGTGVARIIDGHQHKIKQVVGQEVVIKTVL
                     DRDIDKCKTFFGEAVHCTDNFDDILNDEEISIIVELIGYVPAAKDYISKALSAGKHVV
                     SANKDLVALHGKELVNLAKANKCDFLYEAAVAGGVPILRAITESFASDNIQKVMGIVN
                     GTTNFMMTKMDKEGYSYDEALALAQELGFAEKNPTSDVDGLDAARKMVILARLAFGTN
                     DVTLDDVAVTGIRNVSINDIKLANRLGYKIKLIGTAEKIDDSVNVEVGPVFVPESHPL
                     ASVNNEMNAVFVAGEALGETMFYGAGAGELPTATAVVSDVMNIAKNILLETTGNVFNE
                     YEVETLIAKPEQVINPVFMRLEVTDRAGQFLELAKIFATAQVSFDKIIQEPLANGKAI
                     IVIVTHPMSKAQENEIIQAMEDNDDMKLKVHFKVLEH"
     gene            1560..2429
                     /gene="thrB"
                     /locus_tag="PLT73_06590"
     CDS             1560..2429
                     /gene="thrB"
                     /locus_tag="PLT73_06590"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRM20082.1"
                     /translation="MFEIRVPATSANLGPGFDSLGLAVSLYLTLAVKEEADEWEILHD
                     LGAGIPTDKTNLIIETALSLAPNMTPRKITMTSEVPAARGLGSSSAAIVAGIELANQC
                     ADLQLSIDDKIQIATRIEGHPDNVTPAITGDFTVGTVLDSKVYWTKASFPEVALVVTI
                     PEKELLTKESRAVLPDSLPFKEAVKGSSISNMLVAAVFSGDIEKAGALMEQDVFHEPY
                     RGKLVPELSQVRELGHEMGAYGTYLSGAGTTILTMIHPEKAAAFVASAKAAVKNSEVK
                     LLHVEKNGVQVIK"
BASE COUNT          769 a          521 c          687 g          689 t
ORIGIN      
        1 attcatccat ccttgcatga actaaggcag cggaaaaatg agccggctca tttttccgct
       61 gcctttttgt ttttttattt tcattggaat aaagtggcaa acattaaaag tttttaattt
      121 ttatgtagag taattctgta actttcagta tactgtgtaa taaggaaata attgaagagg
      181 tggaataatg gcaaagaaga tttatatagg tttattagga ctaggaacaa tagggactgg
      241 cgttgcccgc atcatagatg gccaccagca taaaatcaag caagtcgtcg gacaggaagt
      301 agtcatcaaa acggttttgg atcgcgatat tgataaatgc aaaactttct ttggcgaggc
      361 tgtccattgc actgataatt ttgatgatat tttgaatgac gaagaaattt caatcatcgt
      421 agaattgatc ggatatgttc cggcagcaaa agattatatt tccaaagcat tatcggccgg
      481 gaagcatgtc gtttcagcaa acaaagatct tgtggcttta catggcaaag aattggtcaa
      541 cctggctaaa gcgaacaaat gcgatttctt gtatgaagca gctgttgcgg gcggggttcc
      601 gattttgcgt gcgatcacgg aaagtttcgc ttccgacaac atccaaaaag tcatgggaat
      661 cgtgaacggc acaaccaact tcatgatgac gaaaatggac aaagaaggct actcttacga
      721 tgaagctctg gcattggctc aggaattggg ttttgccgag aagaacccga caagcgatgt
      781 cgatggattg gatgcagcgc gcaaaatggt cattttggct cgccttgctt tcgggacgaa
      841 tgatgtgacg ttggatgatg tagcagtgac gggtatccgc aatgtttcca tcaatgacat
      901 caagttggcg aaccgtttag gatacaaaat caaactgatc ggtactgccg aaaaaatcga
      961 cgacagcgtc aatgtggaag tcggacccgt gtttgttccg gagtcacatc ctttggccag
     1021 cgtcaataat gaaatgaacg ctgtctttgt cgccggagaa gctcttgggg aaacgatgtt
     1081 ctatggtgca ggagctggcg agttgcctac cgcaacagcc gtagtcagcg atgtaatgaa
     1141 tatcgctaag aacatccttt tggagacaac cggaaatgtc ttcaacgagt atgaggtaga
     1201 gactttgatc gcgaaacctg agcaggtaat caatccggta ttcatgcgct tggaagttac
     1261 cgatcgtgcc ggccagtttt tggaattggc gaaaattttt gccactgcac aagtcagctt
     1321 tgacaagatc attcaagaac ccttggcgaa cggtaaagcc atcattgtca ttgttacgca
     1381 tccgatgtcg aaagcgcaag aaaatgaaat catccaggcc atggaagata acgatgacat
     1441 gaaattgaag gtccatttca aagtactgga acattgattg gatttcgatc ctaatttgaa
     1501 tacattaaaa atcaagaaat gatggaccat cgttcttttg gaaaggattg attagtagga
     1561 tgttcgaaat ccgtgtaccg gcaacttccg ccaatttagg gccaggtttt gattcccttg
     1621 gattggccgt atcattatat ttgacgttgg ctgtaaaaga agaggccgac gagtgggaaa
     1681 ttctccatga tttgggagcg gggatcccga ctgacaaaac gaatctgatt attgaaaccg
     1741 cgttgtcttt ggcgccgaac atgacaccaa gaaaaatcac gatgacgagt gaagtgcctg
     1801 ctgccagagg attgggcagc agttcagctg ccatcgttgc cgggatcgag ttggccaatc
     1861 aatgcgcaga tctgcaattg agcattgatg ataaaattca gatcgcaaca cgcatcgaag
     1921 gtcatcccga taatgtgacg ccagccatca ccggtgattt cacggtcggg accgttctgg
     1981 attccaaagt atattggacg aaggcgagtt tcccagaggt tgccttggta gtgacgattc
     2041 cggaaaagga attgttgacg aaggagagcc gggctgtttt gccggattcg cttccgttca
     2101 aggaagcagt caaaggcagc agcatctcca atatgttagt agcagctgtt ttttcgggtg
     2161 atatcgaaaa agcgggagca cttatggaac aggatgtttt ccatgagcct tatcgcggca
     2221 aattggttcc agagttgagt caagtccggg aattggggca tgagatgggc gcgtacggta
     2281 cttacctgag tggggccggt acgacgatcc tcacgatgat ccatcctgaa aaggcagcag
     2341 cgttcgttgc ttcggcaaaa gctgctgtaa aaaatagtga agtcaagttg ctgcatgttg
     2401 aaaaaaatgg cgtgcaagta atcaaataat tgtgggcccg aggagaaatc cttgggtttt
     2461 ttgtattttt ttggggggcg cccgcaagat taggggcgaa agcgagcaga aaagatgaat
     2521 aacggaaagt tatccatctt tccaggcccg agcgcaagct gacaagtgca aatgggcata
     2581 gcgccatatc aaacgtattc tgaatcccag aaatttgtgc ctaagctgac tatcacacac
     2641 ggacaaaaaa cgtccgttta gtgtta
//
LOCUS       DAOMUJ010000249         2663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11014_length_2663_cov_1.508052, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000249 DAOMUJ010000000
VERSION     DAOMUJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11014_length_2663_cov_1.508052"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLT73_06595"
     CDS             <1..898
                     /locus_tag="PLT73_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM20083.1"
                     /translation="DRILQVLSGNGVFKENYVFYQIRLPRALVLTMAGACLAGAGIIL
                     QTLSNNDLADSGLLGINSGAGLGVTIFYLFAGEGMLQFSAVLPLVAFISALVAASTVY
                     LLSYSRSHKQHAMQLVVIGAGISSAFSGLIILLMSSSDRTDVSFIAAWMSGNIWGSTW
                     PFVFTILPGWLLAMGVLFLKAPMLNILALGEEAAIALGLKLRNEKLILLLCAVAIAST
                     AISLTGNIGFIGLMSPHIAKKLVGKRHERFSWIALLVGSSILLIADAIGRTVMDTAFP
                     TGIVVSLIGAPYFLYLLLGKMR"
     gene            complement(1042..1806)
                     /locus_tag="PLT73_06600"
     CDS             complement(1042..1806)
                     /locus_tag="PLT73_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM20084.1"
                     /translation="MRAYLAFTKKELIEYTRNFKMFLAITLFALLGIMNPLTAKFMPE
                     LLGNFMPEGITILITEPTNLDSWLQFFKNGTQIGLFIIVILLSGMMAKEYEKGTLINM
                     VTKGLPRWTILFSKFTGALLLWTISYWLCFFITLGYTLYYFPEGTTENLTLSVISLYL
                     FGILLIAVLLLGSVLFKNAYGPLLLIGAFLLVLFLWNLFPEAAEWNPLVLVSRNMDML
                     QGTLLLEDLLNPLLITGFIIASSLFTAGKLFSKTTL"
     gene            complement(1806..>2663)
                     /locus_tag="PLT73_06605"
     CDS             complement(1806..>2663)
                     /locus_tag="PLT73_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20085.1"
                     /translation="DDLSFSVPEGSIYGFIGKNGSGKTTTMKIVLGLLPADAGEISVC
                     GEKVHYGDTRTNRFIGYLPDVPEFYGFMTAKEYLRLCGDVTGMPSALIQTKSEELLEL
                     VGLADVKKRIATFSRGMKQRLGIAQALLNEPRLLICDEPTSALDPIGRREILDILLKV
                     KKRTTVIFSTHVLNDVEAICDQVGILDGGKIVLTGSVSELKNTYSRHSCTVTFRTQAD
                     ADLFRTSARINVVGSPASEKHASVTIQSEDPEQTMALIIRILSETGLVPLRVETVEPK
                     LEDIFLEVI"
BASE COUNT          615 a          694 c          700 g          654 t
ORIGIN      
        1 ggaccgcatc ctgcaggtgc tgtcagggaa cggggttttt aaagaaaact acgtctttta
       61 ccagatccgc ttgccccggg ctttggtgct gacgatggcc ggcgcctgct tggcaggtgc
      121 cggtatcatc ctgcagacct tgagcaacaa cgacctggcc gactcgggtc tcctgggcat
      181 caattccggt gccggtctcg gcgtgacgat cttctatctg tttgcgggcg aaggcatgct
      241 gcagttttct gcggtcctgc cgcttgtcgc tttcatcagt gcgctggtgg ctgcctccac
      301 ggtttacctg ctcagttaca gccgcagcca taaacagcat gcgatgcagc tggtggtcat
      361 cggagcgggg atttcctccg ccttttcggg gttgatcatc ctgctgatgt cctcctctga
      421 ccggaccgac gtcagcttca tcgccgcctg gatgtcggga aacatctggg gatcgacttg
      481 gccgttcgtg ttcacaatcc tgcctggttg gctgttggcg atgggtgtcc tcttcctgaa
      541 ggccccgatg cttaacattc tcgcgctggg tgaggaggca gctattgcct tgggattgaa
      601 gctccggaac gaaaaattga ttctgctgct ttgtgcggtc gcaatcgcct caacggcaat
      661 ctcgttgacc ggcaacatcg gcttcatcgg cctgatgtcc ccacacatcg ccaaaaagtt
      721 ggtcggcaaa cggcatgaac gcttcagttg gatcgcgctc ttggtcggga gcagcatcct
      781 gttgattgcc gatgcgatcg gccgcacagt gatggatacc gcttttccga cgggtatcgt
      841 cgtttctctt atcggagcgc cttatttcct gtatttgctg ttggggaaaa tgcgctgagg
      901 atcggtccgc tgacgggcga gagcccaggc cggtgacgga aatacccgaa agttcaaaca
      961 tctgtcgact gatcggatac cgaaaacatc aaaaaacgcg ccatcagccg gaaaagggct
     1021 ggctggcgcg tttttttact ttcataaagt agtcttactg aaaagtttcc ctgcggtaaa
     1081 gaggcttgaa gcaattataa atcccgtaat cagcagcgga ttcagaaggt cctcaagcag
     1141 cagtgtccct tgcagcatat ccatgttgcg cgagaccaac accaacgggt tccactcggc
     1201 tgcttccggg aacagattcc acaagaacag caccaacagg aacgccccga tcaacaacag
     1261 cggtccatag gcatttttga acaggacaga ccccagcagc aaaacagcaa taagcaatat
     1321 accgaacagg tagaggctga tgaccgaaag tgtcaagttt tcggtggtac cttcaggaaa
     1381 gtaatacagc gtgtatccca aagtgatgaa aaagcagagc cagtagctta tggtccaaag
     1441 caacaatgca cctgtaaatt ttgaaaacag gatggtccag cgcggcagtc cttttgtgac
     1501 catattaatg agcgtgcctt tttcgtattc ttttgccatc ataccgctca aaaggatgac
     1561 gatgatgaac agaccaatct gggtgccgtt cttgaagaat tggagccagg aatcgagatt
     1621 cgtcggttcc gtaatgagga tcgtaatccc ttctggcatg aaatttccta acagttcggg
     1681 cataaatttg gccgtcaacg ggttcatgat gcccaacaac gcaaacaaag taatcgcaag
     1741 gaacatctta aagtttcgtg tatattcaat cagttccttt tttgtgaagg caaggtacgc
     1801 tctcatcaga tcacctccag gaagatatct tctagtttgg gttcgaccgt ttccactcgg
     1861 agcggaacca acccagtttc actcaaaatc cgtatgatca acgccatggt ctgttccgga
     1921 tcttcagatt ggatggtgac agaagcatgt ttttcagaag cagggctccc tacgacgtta
     1981 atccgtgcac ttgtccggaa taaatcagca tccgcctgtg tccggaaggt caccgtgcag
     2041 gaatgtctgg agtaagtgtt tttcagttcg gataccgagc ctgtcaggac aatttttcct
     2101 ccgtcaagga tgcctacctg atcgcagatt gcttccacat cgttcaggac atgggtcgaa
     2161 aagattacgg ttgtccgttt tttcactttc agcaggatat ccaatatttc cctacggcca
     2221 atcggatcca gggcggaagt cggttcgtca caaatcaaga gcctgggttc gttcagcaaa
     2281 gcctgggcga tccccaagcg ctgtttcatg ccgcgggaaa acgtggcgat gcgttttttc
     2341 acatccgcca gtccgaccag ttccaacagt tcctcggatt tggtttgaat aagcgcggac
     2401 ggcatgcctg tgacgtcgcc gcacagccgc agatattcct ttgcggtcat gaaaccgtag
     2461 aattcgggta catccggcag gtagccgatg aagcggttcg tccgggtgtc accgtaatgc
     2521 actttttcgc cgcaaacaga aatttcgccg gcatccgccg gcagcaagcc caaaacgatc
     2581 ttcatggtgg tcgtcttccc ggagccgttc ttgccgatga agccgtagat cgagccttca
     2641 gggacggaga aggacaggtc atc
//
LOCUS       DAOMUJ010000250         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11018_length_2662_cov_2.590334, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000250 DAOMUJ010000000
VERSION     DAOMUJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11018_length_2662_cov_2.590334"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLT73_06610"
     CDS             complement(<1..261)
                     /locus_tag="PLT73_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRM20086.1"
                     /translation="MRYHHEIIESDPELPVFYIFHNERKDSGVLAHWHKSLEISYTMS
                     GKISEFTIGGIVHETNAGDILVINSNEVHSIWDDHNKTKALSL"
     gene            595..999
                     /locus_tag="PLT73_06615"
     CDS             595..999
                     /locus_tag="PLT73_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20087.1"
                     /translation="MNFLKNNVISVMYLVLLSTLFMPWILVPDSETFTVLPVTGFRFL
                     SDNLLMLILIFLIIFTYVFSLKSNGLSILILLELELIFLAALLLAPAVIYGLAFFSGV
                     RYGYFLMLIILVSLAFYNYLFITKHIQKRENG"
     gene            complement(1071..2662)
                     /gene="mnmG"
                     /locus_tag="PLT73_06620"
                     /pseudo
     CDS             complement(1071..2662)
                     /gene="mnmG"
                     /locus_tag="PLT73_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
BASE COUNT          622 a          610 c          604 g          826 t
ORIGIN      
        1 gagcgacaaa gccttcgtct tgttgtgatc gtcccagatg gaatggactt catttgaatt
       61 gatgaccagg atgtctccgg cattcgtttc gtggacgatc ccaccgatcg tgaattcact
      121 gatttttccg gacatcgtat agctgatttc cagtgatttg tgccaatgcg caaggacgcc
      181 ggaatccttc cgctcgttgt gaaaaatgta gaacacgggg agttccggat ccgattcgat
      241 gatttcatga tgataacgca tctgctgtgc ctcctatcct tgttgattcg gctcccaaag
      301 acacttgctc tgtttgcgcc tctaaagctc aagctccatt atactataat tttttatgcg
      361 gcaatttcac ttttttgata gttcattgag gggaaaaaac tgtaaacgcc gttatgagac
      421 tattttgcat aaaaatatac caaataaaca tcagcatata tatttcacat gaatataatt
      481 gtgatacaat caacacaagt catcagaagt aacgggtagc acactaataa aaaagcattg
      541 tggaagtata tttctttctt tgtagactaa tttagagatg aggggctgat tggaatgaat
      601 tttcttaaaa ataatgtcat ttccgttatg tatttagttt tgttaagcac tctgtttatg
      661 ccttggattc tggttccaga ttcagaaaca ttcacggttc tgcctgtaac gggttttcgc
      721 ttcctttcgg ataatcttct gatgttgatt ttgatttttt taattatctt tacctatgtt
      781 ttttcactca aatccaatgg cctgagcatc ctgattttgt tggaactgga actcatcttt
      841 ctggcagcac ttttgttggc acctgctgtt atatacggat tagcattttt ttcaggagtt
      901 cgatacggat attttttaat gttaatcatt ctggtcagtc ttgcttttta caactaccta
      961 ttcattacaa aacatattca aaaaagagag aatggctagt ccatcttttt tgattagagc
     1021 aaaaaaagaa gcagaacgcc tgttaatatg agcgttctgc ttctttaatt ttactctgaa
     1081 actttggcga tttttccttg ggtcacgtag accatcaaaa cggaaatgtc ggctgggttg
     1141 acgccgctga tccggcttgc ttgggcgatc gtttccggtt ggatcttctt cagattttgt
     1201 ttggcttcat tggcgattcc gttgattgca tcgtaatcga tgttcacagg gatgcgtttt
     1261 tcttccatct tcttcagctt gtccactttt tcgactgctt tcttgatgta gccggcatat
     1321 ttgatctgca cttctacttg cttgatgacc tctttcggca aagctgcttc aagcggagca
     1381 aaagcgatca gaccttcata tttcagttct ggacgcttca aaatatccgc aaacagaatg
     1441 ccatccttca gcggagcgga attttgttcc gcaaggaaag cctgcagctc ttcagtcggt
     1501 ttcaggcgcg tactttcgag gcgggcgatt tccttttcgt tcttccgtca ccaaaccgat
     1561 ttcacggctc atttcggtca agcggaagtc ggcgttatca tggcgaagca acagacggta
     1621 ctcagcgcgc gaagtcagca agcgataagg ctccatcgtc ccttttgtga ccaaatcatc
     1681 gatcatgacg ccgatatagc catcgctgcg cttcaggatg aaaggttctt tgccttgcac
     1741 agccagtgcg gcattgatgc cggcgatgat gccttgtccg gcagcttctt cataaccgga
     1801 cgtaccgttc gtttgaccgg cagtgaataa gttcttcact aattttgtct ccaaggaaag
     1861 gcgcaactga tgcggtttta cgacatcgta ttcgatggca tagcctgtcc gcatcatttg
     1921 ggcattttcc atccccggaa cggaacggat cagatcaagc tgaacatctt ccggcaatga
     1981 ggtggacaag ccttgcacat agacttcttc gttttcgcgg ccttccggct caaggaagag
     2041 ctgatggcgc ggcttgtcgc tgaaacggac gattttgtct tcgattgatg ggcaataacg
     2101 cgcccctaca ccttccacga tacccgtgaa catcggcgcg cggtggagat tgtcgcggat
     2161 gatttcatgg ctttccgggc tcgtatacgt cagccagcag gataattgat ctttcagata
     2221 gtcttcatct ttggaaagga agctgaaatg attcggctca acgtcaccag gctgttcctc
     2281 ggttttggaa taatcgatgc tcgaagccat cacccgcggc ggcgttccgg tcttgaagcg
     2341 cgccaattca aatcccagat caagcagatt ttcggataat ttgatggacg gctgcgagtt
     2401 gttcggtccg gatgaataaa ccaagtcccc gatgatgatt tttcctcggg aagaggttcc
     2461 ggctgtgatg acgacggccc cagcgcggta aacagcgcct gtatgcgtca ctacaccctt
     2521 gacttcaccg tcttctacga tcaattcttc aacgatggct tgcttcagca gcaagttttc
     2581 ttgtttttcg atggtgtgct tcatgtgatg ggcatagtca tccttatccg cttgtgcgcg
     2641 caatgcttgg actgcagggc ct
//
LOCUS       DAOMUJ010000251         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11060_length_2655_cov_2.603077, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000251 DAOMUJ010000000
VERSION     DAOMUJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11060_length_2655_cov_2.603077"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLT73_06625"
     CDS             complement(<1..827)
                     /locus_tag="PLT73_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM20088.1"
                     /translation="MKNAHRKFLSAATLAGITLLAGCGNANTITFWNPLTGDDGAYMD
                     ALVAEYNETDPEFPVESVITADMYTKIYTVMNSGKDIPDLTLIHADRVPQFADLDMLE
                     PIEGLMATNTDLSADNYLEVAWNAGNYEGTQYTVPLDIHGNAMYYNTDLLDKYDANTF
                     LDDDVVTIEEILSLDGKLDEGQYAINNALIEWVTLANVVNAGGDISDEAGNPTINTDA
                     MRSVVEQLKSVADAGLMSPYGEDGYAMFQSGDVLFSTDGTWTSTAHGSVEGLNFGVT"
     gene            complement(842..2341)
                     /locus_tag="PLT73_06630"
     CDS             complement(842..2341)
                     /locus_tag="PLT73_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HRM20089.1"
                     /translation="MEAKLSVNRQNTISKIDKRLYGSFIEHLGRAVYDGIYEPEHKNV
                     DAEGFRTDVKEVVAELNVPLIRYPGGNFVSGYNWEDGIGPKETRPKKLDLAWRSLETN
                     QVGIHEFAKWAKEVNAEVNMAVNLGTRGIQEAVECLEYCNFDGGTYWSDLRKQNGSAE
                     PFGIKTWSLGNEMDGPWQIGHKTADEYGRLAAETAKAMKLVDDSIELVVCGSSTSKMP
                     TFGDWERIVLEHTYDYVDYLSLHCYYGNKENDIGNYLAQSLDMDRFIKTVVSICDFVK
                     VKKGSDKQINLSFDEWNVWYHSNDQDKELEPWQIAPPLLEDIYNFEDALMVGCLLITL
                     LKNADRVKIACLAQLVNVIAPIMTEKNGDTWKQTIFYPFMQVSNYGRGVVLNPRVESE
                     TYQSKDFAEVPYIETIAVHNDETGELVIFAVNRSEDREIAFTFEEEGFGLESILEATE
                     LAGYDKKDTNATDHDLVKLQEKSDVVLKDDAFSTVLKPLSWNVVRLKTK"
BASE COUNT          674 a          667 c          490 g          824 t
ORIGIN      
        1 ttggtaactc caaagttcaa accttcgact gaaccatgtg ctgtggatgt ccaggttccg
       61 tcagtcgaga acagaacatc tcccgattgg aacatcgcat agccatcttc tccgtaaggt
      121 gacatcaggc cggcatccgc tacggatttc aattgttcca ctaccgaacg catcgcatcc
      181 gtgttgatag taggatttcc ggcttcgtca gaaatatcac cacccgcatt gacgacattc
      241 gctaatgtta cccattcgat caaggcgttg ttgattgcat actggccttc atccaatttc
      301 ccgtccaatg ataggatttc ctcaatcgtt accacatcgt catccaagaa agtatttgca
      361 tcatatttgt ccaacaaatc agtattgtag tacatcgcat ttccgtggat atccaatgga
      421 accgtgtact gggtcccttc ataattcccg gcgttccatg caacttccag gtagttgtca
      481 gcggacagat cggtatttgt cgccatcagg ccttcgatcg gctccagcat atccaaatca
      541 gcaaattgag gaacacggtc ggcatggatc agcgtcaaat ccgggatatc tttgcctgag
      601 ttcatgactg tataaatttt ggtgtacata tcggcagtga tgacactttc tacagggaac
      661 tcaggatctg tttcgttgta ttctgccacc aaagcatcca tgtaagcacc atcgtccccg
      721 gtcaacggat tccagaatgt aatcgtattt gcgttaccgc aacctgccaa taaggtaatt
      781 ccggccaaag tcgctgctga taagaatttt ctgtgtgcat ttttcatttt atttcctcca
      841 tttatttagt tttcaatctg acaacattcc aggatagcgg tttcaaaaca gtcgaaaatg
      901 catcatcttt caataccaca tccgattttt cttgcagttt gaccaaatca tgatcggttg
      961 cgttcgtatc ttttttatcg tatccggcca gctccgttgc ctctaggatg ctttccagtc
     1021 caaatccttc ttcctcaaaa gtaaacgcga tttctctgtc ctcggatctg tttactgcaa
     1081 aaataaccag ttcacccgtt tcatcattgt gcacagcaat cgtttcgatg taaggtactt
     1141 cagcgaagtc cttcgattga tacgtttctg attcaacacg tggattcagc accactcctc
     1201 ttccatagtt ggatacttgc atgaacgggt agaaaatggt ttgtttccat gtatctccgt
     1261 tcttttcggt catgatcggt gcgatgacat taaccaattg ggccagacaa gcaatcttga
     1321 cgcggtcagc atttttcaac aacgtaatca gcaagcagcc taccatgagg gcatcttcga
     1381 aattgtagat atcctccaat agcggcggcg caatttgcca aggttccaat tccttatctt
     1441 gatcattgga gtgataccag acgttccact cgtcaaacga cagattgatc tgtttgtcgc
     1501 tgcctttttt tactttaacg aaatcgcaga tggaaaccac cgttttgatg aagcgatcca
     1561 tatccaacga ttgtgccaga taattgccta tgtcattttc cttatttcca taatagcaat
     1621 gcaaggaaag atagtcgaca taatcatagg tatgttccaa gacaatccgt tcccagtcac
     1681 cgaaagtcgg cattttgctg gtcgaacttc cacaaacgac caattcgatg ctgtcatcga
     1741 ccaatttcat cgctttagcc gtttctgccg ccaagcgtcc gtattcgtct gcggtcttat
     1801 gaccgatctg ccatggccca tccatttcat tgccgagcga ccaggtcttg attccgaaag
     1861 gttctgctga tccgttctgc ttgcgcagat cgctccaata ggttccgccg tcaaaattgc
     1921 agtattccaa gcattccaca gcttcctgga tgccacgcgt gcctaaattg actgccatgt
     1981 tcacttctgc attgacttct tttgcccatt tggcgaattc atggatgcct acctgatttg
     2041 tttccagcga gcgccacgcc aagtccaact tcttcggacg cgtctctttt ggcccgattc
     2101 cgtcttccca gttgtacccc gatacaaaat tgcctccagg ataccgtata agcggtacat
     2161 tcagttcggc cactacctct tttacatctg tccgaaagcc ttctgcatcg acatttttgt
     2221 gttccggttc gtaaatgccg tcatatacag cccttcccaa gtgttctatg aacgaaccat
     2281 acaaacgctt atctattttg ctgatggtat tctgcctgtt gaccgataat ttcgcttcca
     2341 tttgtcctcc ttaccaactt agttaacact ttagtttact aagattaata aaattaactt
     2401 atgaggtaat aatatcgaac tatgcaaacg ttgtcaacac gaatttctcc tttttagcaa
     2461 atatatccca agtttaatca acacatttgt taatcatgtc cataaataca gtcatcacgc
     2521 tcttttcact cagtattttc ataaaattgt gtgaaatata tctttttttg gttaactcat
     2581 atagtaatgt aatccaacct attgacagac acgtgataaa cttttatatt tatcacatac
     2641 agtgatgaac aatat
//
LOCUS       DAOMUJ010000252         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11110_length_2646_cov_1.531841, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000252 DAOMUJ010000000
VERSION     DAOMUJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11110_length_2646_cov_1.531841"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1732)
                     /locus_tag="PLT73_06635"
     CDS             complement(<1..>1732)
                     /locus_tag="PLT73_06635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRM20090.1"
                     /translation="IYRGAYIINWDPKAKTALSDIEVIHKDVQGAFYHFRYPMTDGSG
                     YLELATTRPETMLGDTAVAVHPEDERYQQFIGKTVTLPLVNREIPVIADDYVEMDFGT
                     GVVKITPAHDPNDFEVGLRHDLPQVNVMNDDATMNEAAGKYAGMDRFAACKAIVKDME
                     ELGYLVKIEEMTHSVGHSERTNVVVEPRISTQWFVKMQPLAERAVENQSAEEKVSFYP
                     ERFENTFLTWMGNVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPSDSENWTQDED
                     VLDTWFSSALWPFSTMGWPDEDAEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTE
                     KRPFENVLIHGLIRDEQGRKMSKSLGNGIDPMDVVEKYGADALRWFLANGSAPGQDVR
                     FSYEKMDASWNFINKIWNASRYVLLNLEDLTFDAIDISGEKTIADKWILSSLNKTITK
                     VTDLFEKFEFGEAGRLLYRFIWDDYCDWYIEMSKEVLQGENEAAKKTTRSILAYVLDN
                     VLRLLHPVMPFVTEEIWQNVPHQGDSIVTAAYPVADASHIDEAAEEAMEKLIELIRGV
                     RNVRAEMNTPL"
     assembly_gap    1733..1756
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1757..2266)
                     /locus_tag="PLT73_06640"
     CDS             complement(<1757..2266)
                     /locus_tag="PLT73_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM20091.1"
                     /translation="MSKADAMPTKYQPQQVESGKYQKWVEAGLFKPSGDKSKKPYSIV
                     IPPPNVTGRLHLGHAWDVTLQDMLIRQKRMQGFDTLWLPGMDHAGIATQAKVEAKLAE
                     DGLTRYDLGREAFVDKVWEWKEDYASVIREQWGKMGISVDYDRERFTLDDGLSDAVRK
                     VFVGLYEKGL"
     misc_binding    complement(2328..2552)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          610 a          721 c          568 g          723 t
ORIGIN      
        1 acaacggtgt gttcatttca gcgcggacat tgcggacgcc acggatcaat tcgatcagtt
       61 tctccatcgc ttcttcagcc gcttcatcga tatggcttgc atccgctacc ggataggctg
      121 ccgtcacgat ggaatcacct tgatgcggta cgttctgcca gatttcttcg gtgacgaaag
      181 gcataaccgg atgcaacaag cgcaagacgt tatccaaaac ataagctaag atgctgcggg
      241 ttgtcttttt agcagcttca ttttcccctt gcaggacttc tttggacatt tctatatacc
      301 agtcgcagta atcgtcccag atgaaacggt acaacaagcg accggcttca ccgaactcaa
      361 atttctcgaa caggtccgtc actttagtga tggtcttgtt caggctggaa aggatccatt
      421 tatcggctat cgtcttttcc cctgaaatgt cgatagcgtc gaatgtcagg tcttccaagt
      481 taagaagcac gtaacggctg gcattccaga ttttgttgat gaagttccag gaggcatcca
      541 tcttctcata gctgaagcgg acatcctgac caggtgcgga accgttcgcc aagaaccaac
      601 gcaaagcatc agccccgtat ttttcgacca catccatcgg gtcgatcccg tttccgagcg
      661 atttgctcat cttgcggcct tgctcgtcac ggatcagacc atggatcagt acattctcaa
      721 acggacgttt ttcggtgaac tccagactct ggaagatcat ccggcttacc cagaagaaga
      781 tgatgtcata gccggtaacc aacgtactgg tagggaaata acgtttgaaa tcttccgcat
      841 cttcatccgg ccagcccatc gtcgagaacg gccaaagtgc tgaactgaac catgtatcca
      901 gaacatcctc atcctgcgtc cagttttcgc tgtcgctcgg ggcttccatg cctacgtaca
      961 tttcgccggt ttctttatga taccaagccg ggatttgatg tccccaccat aattgacggg
     1021 agatgaccca gtcatggacg ttgcccatcc acgtcaggaa agtattctca aatcgttccg
     1081 gatagaagga aactttttct tctgcggatt gattttcgac cgcacgctca gccaatggtt
     1141 gcattttgac gaaccattgc gtcgagatgc gcggttccac aacaacgttt gtgcgttcag
     1201 aatgtccgac actgtgggtc atttcttcaa ttttgaccaa atagcccagt tcttccatat
     1261 cttttacgat cgctttgcac gctgcgaaac ggtccatacc tgcatatttt ccggctgctt
     1321 cgttcatcgt cgcgtcatcg ttcatgacat tcacttgcgg caagtcatga cgcaaaccga
     1381 cttcaaagtc gttcgggtca tgagccggtg ttatttttac gacaccggtc ccgaagtcca
     1441 tttcgacata atcatccgca atgacaggga tttcccgatt cacgagcggc aaagtcacgg
     1501 tcttgccgat gaactgctga tatctttcat cttccggatg aaccgctacg gcagtatctc
     1561 caagcatcgt ttccggacgg gtagtagcca gctccaagta tcccgaacca tccgtcatcg
     1621 gatagcggaa gtgatagaag gcgccttgga cgtccttatg gataacttca atatctgata
     1681 aggccgtttt tgcctttgga tcccagttga tgatgtatgc gccgcggtag atnnnnnnnn
     1741 nnnnnnnnnn nnnnnncaaa cctttttcgt acaagccgac gaagacttta cggactgcat
     1801 cggacaaacc atcatccaac gtgaaacgct cccggtcata atcaacggag atccccattt
     1861 ttccccattg ctcgcggatg acgcttgcat aatcctcttt ccattcccaa accttgtcga
     1921 caaaggcttc acgaccaagg tcataacggg taagcccgtc ctcggccagt ttcgcttcca
     1981 ctttggcttg ggtggctatg ccggcgtggt ccatacccgg caaccataac gtatcgaacc
     2041 cttgcatgcg tttttggcgg atcagcatat cctgcagcgt cacatcccaa gcatggccca
     2101 agtgcagcct gccggttacg ttcggaggcg gaatcacgat cgaatacggt tttttgctct
     2161 tgtctccgct aggcttgaaa agccccgctt ccacccattt ttgatatttc ccagattcta
     2221 cttgctgggg ttgatacttt gtcggcattg cgtctgcttt agacattcag ccacttcctc
     2281 tctctttaca ttgatttatt gcgaaaaaaa acgccctgca tacgaatatg caggacgata
     2341 tgaatcgcgg taccacctaa attacaggaa acggatccct gtctctcttt ttgataacgg
     2401 agaatactcc gagctgcgct acccagtcca cagaccttca cacaacttgc tcacaagcta
     2461 cttcactttc cttccccaaa aaagctttca ccaatcgctt ttctctcttg tatggttccg
     2521 ggtaagctac tcttcttgat cgacgctttg tttatacttc actagtttag caccttttca
     2581 ttgaaatctc aatgcgatta aagtaatttt ttggcagctg ccaatgctgc ttcctcatcc
     2641 ggctcc
//
LOCUS       DAOMUJ010000253         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11134_length_2642_cov_2.274836, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000253 DAOMUJ010000000
VERSION     DAOMUJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11134_length_2642_cov_2.274836"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..1285)
                     /locus_tag="PLT73_06645"
     CDS             complement(314..1285)
                     /locus_tag="PLT73_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM20092.1"
                     /translation="MDLIALLSQESVIEKEQKKRHKFVPDLPELEGEATRAPKIKEHL
                     FFENKDIYISKHNRYAAYPEHSHQFLELNYIVKGECRQIINGVPYLLKEGDILLMDTG
                     SAHSIEALGKDDLLLNILFNNKSISINWLMNMNHHDSILYKILLTNNPLDTNAANFIL
                     FRNEQNEHIKQIINNMADEYFFPKVFSGKIISSYLPILIYELARSLPHEYSETFSKKD
                     PFYEVLQLIDAEFTTLTLDAASKRLNFNKNYLSNMIKKRSGQTFTELLNEKRLLKANL
                     LIESTEIPIQTILTEVGFSNKTYFYTCYKNRFHCLPSEVRKNGAASK"
     gene            1403..>2642
                     /gene="rhaB"
                     /locus_tag="PLT73_06650"
     CDS             1403..>2642
                     /gene="rhaB"
                     /locus_tag="PLT73_06650"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HRM20093.1"
                     /translation="MKYYTMIDIGASSGRIMLAEIDNKQLALQEVHRFKNGFSRIDGS
                     DRWDIETIFEEILTGLSKLKALGVTECHLGIDTWAVDYCLIGTDGQLLQLPISYRDER
                     TKDTMEKVAQEIDKETIYAKTGIQFLNFNTLYQLYEEDKDLLAKTDKILMVPDYLAYR
                     LTGEMVGEVTNVSSSQMLNLETGEFDADLLELVGIPREKFPELVVPGTKIGDVKADLT
                     ESYDLPKIEVIAAATHDTASAIVGVPALGGKWAYLSSGTWSLIGVENDAPINDRPAYE
                     ANFTNEWGAYGTYRFLKNITGMWFVQEIARNLDYRHSYGEMAQMAYAVEPFLQYVDLN
                     DPRFLNPAKMIAEIQAYCRERGEIVPETTGELVMCVYSNLALAYAHELKKVEALTQET
                     IDVLHIVGGGANVKLLNQLTA"
BASE COUNT          685 a          598 c          712 g          647 t
ORIGIN      
        1 gccgcgattc ctggaattgg caaaagaaca atccgaacgg ctacgaatga aaaaagctta
       61 tacagactcg ttgattctgt atgagaattg aatatatgct caaaaatagc ccgccaaaag
      121 tactttggcg ggctatttca gtccaaacaa cagtcataaa acccgccgaa accactttgg
      181 cgggggaaaa cgtccgcgga caactcgaga ttcaccgcca aatccatttt gatggagaat
      241 ctcgccacga acttcgaacc ccagagacaa acagataaca ataccattaa acaacaaaag
      301 aaacggagca gtctcatttc gaggcagctc cgttttttcg cacttccgat ggcagacagt
      361 ggaatctgtt tttgtaacac gtatagaaat aagtcttgtt cgaaaagccg acttcggtca
      421 ggatcgtctg gatcgggatt tcggtggatt cgatgagcag gttggctttc agcagccgct
      481 tttcgttcag tagctccgtg aacgtctggc cgctgcgctt cttgatcatg ttgctcaagt
      541 agtttttgtt gaagttcagc cgtttggatg ccgcgtccaa ggtcagggtc gtgaattcgg
      601 catcgatcag ctggaggacc tcataaaagg ggtctttttt gctgaacgtt tcgctgtact
      661 cgtgcggcag cgaacgtgcc aattcgtaaa tcaggatcgg cagataactg ctgatgatct
      721 ttccggaaaa gactttcggg aagaaatatt catcggccat attgttgatg atttgcttga
      781 tgtgttcgtt ttgttcattg cggaacagga tgaagttggc tgcattcgtg tccagtgggt
      841 tattcgtcag caaaattttg tagaggatgc tgtcgtggtg gttcatgttc atcagccagt
      901 tgatcgagat gcttttgttg ttgaagagga tgttcaaaag cagatcgtcc ttgccgagcg
      961 cttcgatgga atgcgcgctg ccggtgtcca tcagcaggat gtcgccttcc ttcaacaagt
     1021 aagggacgcc gttgatgatc tggcggcatt cgcccttcac gatgtagttg agttcgagga
     1081 attggtgcga gtgctccggg taggcggcgt agcggttgtg tttgctgatg tagatgtcct
     1141 tgttttcgaa aaacagatgt tccttgattt ttggcgcccg ggtcgcctcc ccctccagtt
     1201 ccggcagatc gggcacaaat ttatgccgtt ttttctgttc cttttcgatg acagattcct
     1261 gcgataacag cgcaatcaga tccattcgaa cacctcctgt atatttggta ctcacaactt
     1321 attcagtgac attgtagcat aaatccagaa gttgtttaat agtaatgtaa acaattacaa
     1381 ggcggaggtg tgaaacaagt aaatgaagta ctatacgatg atagatatcg gagcctccag
     1441 cgggcggatc atgctggcgg aaatagataa taaacaattg gcgttgcagg aagtgcaccg
     1501 tttcaaaaat ggcttcagcc gcatcgacgg atccgacaga tgggatatcg aaaccatttt
     1561 cgaagaaatc ctgactgggc tatccaaact gaaggcactc ggcgtgacgg aatgccatct
     1621 cgggatcgat acatgggcgg tcgactactg cctgattgga acggacggcc aattgctgca
     1681 gctgccgatc agttaccggg acgaacgcac gaaagatacg atggaaaaag tggcgcaaga
     1741 gatcgacaaa gagacaatct acgccaaaac gggcatccag ttcctgaact tcaatacttt
     1801 gtatcaactg tatgaagagg acaaggactt gttggccaag accgacaaaa ttttgatggt
     1861 accggattat ctggcatacc gcttgaccgg ggaaatggtc ggcgaagtga cgaatgtgtc
     1921 ttcttcgcag atgttgaatc tcgaaaccgg ggagttcgat gccgacctgc ttgaacttgt
     1981 gggcatcccg agagagaagt tcccggaact ggttgtgcca ggaacgaaaa tcggcgatgt
     2041 gaaggctgat ctgacggaaa gctatgattt gcccaaaatc gaagtgattg ctgcagcgac
     2101 gcacgatacg gcatccgcca tcgtcggcgt gccggcgctg ggagggaaat gggcctacct
     2161 cagcagcggc acctggtcgt tgatcggtgt ggaaaacgat gctccgataa acgaccgtcc
     2221 cgcctacgaa gcgaacttca cgaatgagtg gggcgcttac gggacctatc gtttcctgaa
     2281 gaacatcacc ggcatgtggt tcgtgcagga aatcgcgcgc aacctggatt acagacattc
     2341 ctacggggaa atggcgcaga tggcttatgc agtcgaacca ttcctgcagt atgtcgacct
     2401 gaacgatccg cgcttcctca atccagcaaa aatgattgcg gaaatccaag cctattgccg
     2461 cgaacggggc gaaatcgttc cggaaacgac cggcgaattg gtgatgtgcg tgtacagtaa
     2521 tctggccttg gcctatgcgc atgaactgaa gaaagtcgaa gcactaacgc aggaaacgat
     2581 tgacgtgctg cacatcgtcg gcggcggagc gaatgttaaa ttgctgaatc agttgactgc
     2641 cg
//
LOCUS       DAOMUJ010000254         2640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11144_length_2640_cov_2.123791, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000254 DAOMUJ010000000
VERSION     DAOMUJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11144_length_2640_cov_2.123791"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLT73_06655"
     CDS             <1..403
                     /locus_tag="PLT73_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010497035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRM20094.1"
                     /translation="KGKTHSILNRLSDYFKPEFINRFDAIVEFNQLSKENLSRIVTLM
                     LDDVNELLKDKEISVTVSQKAKDRLIDLGYDPSMGARPLRRVIQDQIEDQVAEFYLDN
                     PKIKDLFVDADEEGNLMVVSKVDQPETTTSE"
     gene            564..839
                     /locus_tag="PLT73_06660"
     CDS             564..839
                     /locus_tag="PLT73_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HRM20095.1"
                     /translation="MKLIDVTNSHADLVREQLANTDAQFVKVYSLGQTTVIFTGAATH
                     EDVIILNKNRRVKQSEIDFVLTKLLHLTVDDVEVMPGHNFVELSHLK"
     gene            complement(932..2509)
                     /locus_tag="PLT73_06665"
     CDS             complement(932..2509)
                     /locus_tag="PLT73_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM20096.1"
                     /translation="MTHTLADKVKARRTFAIISHPDAGKTTITEQLLLFGGAIRQAGT
                     VKGKKSGKFAKSDWMDIEKQRGISVTSSVMQVDYDGKQINILDTPGHEDFSEDTYRTL
                     MAVDSAVMVIDSGKGIEPQTKKLFKVCSMRGIPIFTFINKLDRDGREPMDLIAELEEV
                     LEIDAYPMNWPMGMGKNLLGLYDIYNNRVELTHPEENEDNDFLPLNEDGEIEGDYQVT
                     QSTIYTQAVEDVQLLKEAGNDFSEEAIAAGKLTPVFFGSALTGFGVQTFLDAFVDFAP
                     SPSEHLTVADEIVEPTDENFTGFIFKIQANMNPAHRDRIAFVRVCSGEFKPGMDVFVT
                     RTGKKIKLAHTTQFMADSREQVQTAVAGDIIGLYDTGNFQIGDTIHEGKKALEFEALP
                     QFTPELFMKVTPKNVMKQKSFHKGVQQLVQEGAIQLYKTYHTEDYILGAVGQLQFEVF
                     QYRLLHEYNAEVEMTPMGSKIARWIDPEDLDPNMSSSRNLLCRDRFGDPVFLFENQFA
                     MRWFEDKYPEVKLTSLL"
BASE COUNT          723 a          627 c          528 g          762 t
ORIGIN      
        1 gaaaggcaag acgcattcca ttctgaatcg cttgagtgat tacttcaagc ctgaattcat
       61 caaccgcttc gatgcgattg tcgagttcaa ccagctttcc aaagaaaatt tgagcagaat
      121 cgtcaccctt atgttggatg acgtcaacga attgctgaag gacaaggaaa tcagcgtcac
      181 cgtctctcag aaagcaaaag atcgcctaat cgatctggga tatgacccaa gcatgggcgc
      241 tcgcccgctg cgccgcgtta ttcaagatca gattgaggat caagtagctg aattctactt
      301 ggataatccg aaaatcaaag atttatttgt cgatgcagac gaagaaggta atctgatggt
      361 cgtcagcaaa gtcgaccaac cagaaacaac gacttctgaa taatattcgg ttccgaaagg
      421 caagagccca aaagtcagct cttgcctttc tcattttaaa aatccaagaa aattattcat
      481 ttgtttgttt actctttaaa taaattaaca gttgttatat aattattcta taaacagcca
      541 acctaagtta ggggtgttcg tccatgaaat tgatcgatgt tacaaatagc cacgctgatc
      601 ttgtacgtga gcaattggcc aacaccgatg ctcaatttgt caaagtttat tctttgggac
      661 aaactacagt catctttacc ggtgccgcaa cgcatgagga tgtcattatc ctcaacaaaa
      721 accgtcgtgt gaagcaaagt gaaatcgact ttgtgctgac gaaattgttg catcttaccg
      781 ttgatgacgt ggaagttatg cctggtcata acttcgtgga gctatctcat ctcaaatgat
      841 tgacattttt ggcactcgaa aaaaactctt cttaacataa aaacgcacac tgcttttcag
      901 caatgtgcgt tttttgtttc ctttgatcaa attaaagtaa agatgtcaat ttgacttctg
      961 gatatttatc ctcgaaccaa cgcatagcaa attgattttc gaaaaggaac acaggatccc
     1021 caaaacggtc gcggcataat aagttacggc tggatgacat attcggatcc aaatcttccg
     1081 gatcgatcca tcgcgcaatt tttgagccca tcggtgtcat ttctacttct gcattgtatt
     1141 cgtgcaacaa acggtactgg aacacttcaa actgcaattg accgaccgca cccaaaatat
     1201 aatcttctgt atggtacgtt ttgtacaact ggatggcccc ttcttgaacc aactgctgca
     1261 cccctttatg gaaggatttc tgtttcatga cgtttttcgg agtgactttc atgaacagtt
     1321 caggtgtaaa ttgcggcaat gcttcaaact caagtgcttt tttgccttca tggatcgtat
     1381 cgccgatctg gaagttccca gtatcgtaaa gtccgatgat atcacctgct acagcggtct
     1441 ggacttgctc acgggaatcg gccatgaact gcgttgtatg cgccaacttg atttttttgc
     1501 cggtacgggt cacaaaaaca tccatcccgg gtttgaattc accggagcag acacgcacga
     1561 aagcaatcct atctcggtga gcgggattca tatttgcttg gattttgaag atgaagccgg
     1621 tgaagttttc gtcagtaggt tcaacgattt catccgccac cgtcagatgc tcacttggac
     1681 tcggagcaaa atccacaaag gcatctaaga acgtctgaac gccaaagccc gttaaagcgg
     1741 aaccgaagaa gaccggcgtc agtttaccgg ctgcaatcgc ttcttcagaa aaatcgttac
     1801 cagcctcttt caataattgg acatcttcaa ccgcttgggt atagatggtt gattgcgtca
     1861 cttgataatc gccttcgatt tcgccgtctt cattcagcgg aagaaaatca ttgtcttcat
     1921 tctcttcagg atgagtcaat tctacgcggt tattgtaaat atcatacaga cccaacaaat
     1981 ttttacccat gcccatcggc cagttcatcg gatacgcatc gatctccaac acttcttcca
     2041 attccgcaat cagatccatc ggctcgcggc catcacggtc caatttattg atgaaagtga
     2101 agatcggaat ccctcgcata ctgcagactt taaataattt ctttgtctgc ggctcaatcc
     2161 ctttcccgct atcgatgacc atcactgcac tatccacagc catcagagta cggtatgtat
     2221 cttccgagaa atcctcatgt ccgggcgtat ccaaaatatt gatctgcttt ccgtcgtaat
     2281 caacttgcat gaccgaactg gttacagaaa tacccctttg tttttcaata tccatccaat
     2341 ctgatttggc gaatttccct gatttttttc cctttaccgt cccggcttga cggattgcgc
     2401 ccccaaacaa cagcaactgc tccgtaatgg tcgttttacc ggcatccgga tgggaaatga
     2461 tggcaaatgt tcttcttgct ttaactttat ctgctaatgt atgagtcata tttttcccct
     2521 tttttccaaa tatctctatc gtgaatgtaa gaaaagctga gcgggttcgc ttagccctga
     2581 gcatggaaac actgcaatct gatcgttctc atttccgcag ggcaaacccg tggagctagc
//
LOCUS       DAOMUJ010000255         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11162_length_2636_cov_2.543588, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000255 DAOMUJ010000000
VERSION     DAOMUJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11162_length_2636_cov_2.543588"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLT73_06670"
     CDS             complement(<1..630)
                     /locus_tag="PLT73_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM20097.1"
                     /translation="MSKEQLNKAFFAQTEEEVLAELNSTRDGLTKEEAAKRLAEYGEN
                     VLEAGEKRTTLQKFLDQFKDFMILVLLAAAIISGTIGHEIADAIIILAVVIINAFLGV
                     FQENKAEEAIEALKKMASPLANVKRDGEVVQVKSELLVPGDIIVLEAGDVVPADIRLY
                     DVNSLKVEEAALTGESVPVEKDLRDVEPDAALGDRKNMAFSSTNVTYGRA"
     gene            complement(947..2410)
                     /locus_tag="PLT73_06675"
     CDS             complement(947..2410)
                     /locus_tag="PLT73_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM20098.1"
                     /translation="MENFFKLKEHGTTVSKEVVAGITTFFAMSYIIFVNPAILSLSGM
                     PSQAVFLATIISAAVSTLVMGLFANVPYALAPGMGLNAFFTFTVVFSLGFTWQQALAM
                     VFICGMVNVLITVTKIRKMIIKSIPESLQHAISGGIGIFIAYIGIKNAGFLEFTSDAG
                     SITAINGAPYDATAETFTGGVGSVVTGGGIVPALVNFTQMPALLALIGLVITAVLMVK
                     NVRGAILIGIVATTLLGIPMGVVDISTASLQANSLATAISELGVTFGAAFGSEGLLSL
                     FSDVSQLPLVLMTIFAFSLSDTFDTIGTFIGTGRRTGIFSAEDEKALEEGSGFSSKMD
                     KALFADSVGTVIGSIFGTSNTTTYVESAAGIGAGGRTGLTAVVVAGMFIISAFFAPLI
                     GVVPAAATAPALILVGILMMGSFTEINWHDLEDAIPAFFASIFMGLSYSISYGIAAGF
                     FFYCIVKVVKGKSYEIHPIVWFSSALFLANFIILALL"
BASE COUNT          794 a          588 c          577 g          677 t
ORIGIN      
        1 cgcacggcca taagtaacgt ttgtgcttga gaacgccatg ttcttgcggt cacctaaagc
       61 agcgtcgggt tcaacgtcac gcaagtcttt ttcgaccggt acagactcac cagtcaaagc
      121 agcttcttcg actttaaggg aattgacatc gtaaaggcgg atatctgcag gaacaacatc
      181 tccggcttcc aaaacgatga tgtcaccagg cacaagcaat tctgacttga cttggacaac
      241 ttctccgtca cgttttacgt tggcaagcgg tgaagccatt ttcttcaaag cttcgattgc
      301 ttcttcggct ttgttctctt ggaatacacc taagaatgcg tttatgataa caacagctaa
      361 gatgatgatg gcatcagcga tttcatgacc gatagtacca gaaatgattg ctgcagctaa
      421 tagaacgaga atcatgaaat ccttgaattg gtccaagaat ttttgcaaag tggtgcgttt
      481 ttcacctgct tctagtacgt tctctccata ctcagccaat ctcttcgcgg cttcctcttt
      541 ggtaaggcca tctctagtcg agttaagttc agctaagact tcctcttccg tttgcgcgaa
      601 gaacgcttta ttcagttgtt cttttgacac agtatttccc ctctcttaac ctgaatgtag
      661 tagtgttaaa atgaaaagag acctatgcat aaaattgtcc atcagacaac actgtatgca
      721 taagtctcac tatttaaggt aacaccagca gataggatta ctctacttat tgttgacatt
      781 accacagcct tgtactgcta gttactcccc tatggagata ttcagtttaa cactcggatt
      841 aattatatcg acaaaagcaa ggaatcgcaa caatttatta ccgaaaacga aaacaattaa
      901 aaaaaaagac acatttccgt atttttcgga aatgcgccca gtcatattaa agcaatgcca
      961 aaatgatgaa gtttgccaag aataaagccg aagagaacca aacgatcggg tggatttcgt
     1021 acgactttcc ttttacgact ttcacgatgc agtagaagaa gaagccggct gcgataccgt
     1081 aggaaatgct gtaagaaaga cccatgaaga tggatgcgaa gaaagcaggg attgcatctt
     1141 ccagatcatg ccagttgatt tccgtgaatg atcccatcat caaaataccg acaaggatca
     1201 aagcaggagc tgttgcagct gcgggaacaa caccaatcag cggtgcgaag aatgcactga
     1261 tgatgaacat gccggcaact acaactgcag tcaaaccagt acgtccgcca gcaccgatac
     1321 cggcagcact ttcaacgtaa gtagtcgtat ttgaagtgcc gaagatcgaa ccgattaccg
     1381 taccaactga atcggcgaac aaagctttat ccatcttcga actgaatccg gatccttcct
     1441 caagcgcttt ttcatcttct gcagagaaga taccagtacg acggccagtt ccgatgaatg
     1501 taccgattgt gtcgaaagta tcggaaaggc tgaatgcgaa gatggtcatc aatactaaag
     1561 gcaattggga aacatcggag aacaaggaca aaagaccttc tgaaccgaat gcagcaccga
     1621 atgttacacc tagttcggaa atagctgttg caagcgagtt tgcttgcagg cttgctgttg
     1681 aaatgtcaac aacacccatc gggataccca acaaagtggt cgcaacgata ccgataagga
     1741 ttgcgccgcg gacatttttg accatcagta cagccgtaat gaccaaacca atcaaagcca
     1801 ataaagcagg catttgtgtg aaattaacta aagcagggac gatgccgccg ccagttacga
     1861 ctgatcctac tccgccagtg aaagtctcag ccgttgcgtc gtaaggagcg ccattgatcg
     1921 ctgtgatgga gccagcatcg gaagtgaatt caaggaagcc ggcgtttttg attccgatat
     1981 aagcgatgaa gatgccgatc ccgccgctga ttgcgtgctg caggctttca gggatcgatt
     2041 tgatgatcat ttttctgatt ttggttacag tgatcagaac gttgaccatc ccacagatga
     2101 ataccatggc caaagcttgt tgccaagtga aaccaagtga gaagacgaca gtgaaagtga
     2161 agaaggcgtt caagcccata cccggtgcca aagcataagg tacgttggcg aaaaggccca
     2221 tcaccaaagt gctgactgca gcggaaatga ttgttgccaa gaagactgct tggctaggca
     2281 tcccagataa agaaaggatt gctggattga cgaagatgat ataagacatt gcgaagaaag
     2341 ttgtgatccc tgcaacgact tctttggaaa ctgttgttcc gtgctctttg agtttaaaga
     2401 aattttccat tttgaaaaga tctcccatct gaattttttt cggcagcgac aggaaacaga
     2461 tacaataaat gatttcaaat gcattccttg cttgccactc gactattata accaatcacc
     2521 ctaaggcttg caataaataa gtgaatgatt taatcaattt gttagtttaa tgttcgtgat
     2581 ttgcctgatc cagtaatgaa agcggttcaa aagcgctgct attacttctt aacgtt
//
LOCUS       DAOMUJ010000256         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11283_length_2614_cov_2.016413, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000256 DAOMUJ010000000
VERSION     DAOMUJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11283_length_2614_cov_2.016413"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLT73_06680"
     CDS             <1..191
                     /locus_tag="PLT73_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM20099.1"
                     /translation="HASLIQKEAAGEKVEFSLIIMHAEDQMMAAETTKLLIEEMIEMF
                     KQLKGTEGNTEKKEAGSL"
     gene            366..1409
                     /locus_tag="PLT73_06685"
     CDS             366..1409
                     /locus_tag="PLT73_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20100.1"
                     /translation="MNSTEIAKLAGVSRSTVSRVINNYSNVPEETREKVLKVIKEHDY
                     VPHASARMLAGSKNKVIGLFIVDLINEEDGLKNRITKSPYYLEFTSSVIETASEMDYL
                     VLVHIIHTVAGYEKIKECFYNKMVSGGIFIGQNDDDESIKTIIERGYRVVLVDQSVRP
                     DDVTYNQCTIVNADNYGGAYRATKYLTDMKHKQIAHITGGTAKFSSKDRIRGYKKALE
                     DAGIKVNKDLIINSEFVENAGYEATKKLFNGKSKFSAIFASNDKIAFGAIKAIQEAGL
                     KVPEDISVIGFDDIESSKYFNPPLTSIKMELVEMADLATKSIVASIEQDVKPSVNIVP
                     VTLVERESCALLK"
     gene            1524..2414
                     /locus_tag="PLT73_06690"
     CDS             1524..2414
                     /locus_tag="PLT73_06690"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRM20101.1"
                     /translation="MAYEPKTKPTEVSVEKFIEEIQQPQKKEDAYALLALFAEVTGEE
                     AKMWGPSIIGFGKYHYRYASGHEGDAPLAAFSPRKTALTFYFMLPDGKREELLAKLGK
                     HKTGKGCVYVNKLSDIDTAVLKEMIREDIAHATQLYGGEAADKALPASASIAKRLGFE
                     KFQKRAVLGKERAVADDFAELDSYDTDVDAGKYDLIFSYVLTLEELKARVWDTINHDR
                     LNPEGYLYIAYPKIGNKSYDTSVHRDAIFPSLGVDDGKGTVGDSTLKFARLVKLDDTF
                     TLVGMKNAVKSKDHKTKNLY"
BASE COUNT          809 a          512 c          662 g          631 t
ORIGIN      
        1 cacatgcctc cttaatacaa aaagaagctg ccggtgaaaa agttgaattc tcgctgatca
       61 tcatgcatgc agaggatcaa atgatggcgg cagagacaac gaaattactg atcgaagaaa
      121 tgatcgaaat gttcaagcag ttaaagggta cagaaggtaa tacagaaaaa aaagaagcag
      181 gctcactata atttttgcgt cactcagaca cgaaaactgt ttttgatggt gagatagctc
      241 aaagcattca gggcacccgg aatcctcctg ggtgtcttat tttttttaaa aagattgaaa
      301 aaactagtca aatggtatag tgtttgatta tgaacacgag tttatgaatg agaggggatg
      361 cgtttatgaa tagtactgaa attgcaaaac ttgcaggtgt atcccgcagt actgtatcca
      421 gagtaatcaa taattattca aatgtcccgg aagaaacaag agaaaaagtc ttaaaggtaa
      481 tcaaagaaca tgattatgtt ccgcatgctt cagcaaggat gctcgcgggg agcaagaaca
      541 aagtgatcgg actttttatt gtagatctga tcaacgaaga agacggacta aaaaacagaa
      601 tcaccaaaag tccgtattat ttggagttta ctagttccgt gatagagaca gccagtgaaa
      661 tggactactt ggtccttgtt catatcatac acactgttgc aggttatgaa aaaatcaaag
      721 agtgcttcta caataagatg gtctctggag ggatttttat aggccagaat gatgatgatg
      781 aatcgataaa gacaataatc gaaagaggct acagagttgt attggttgat caatcagtcc
      841 gaccagatga tgtcacatac aatcagtgca cgatagtgaa tgctgacaat tacggtggtg
      901 cctatcgtgc aacgaaatat cttacagata tgaaacataa acaaatcgca cacattacag
      961 gtggtacggc taaattctca tccaaagacc gaatcagagg atacaaaaaa gccctggaag
     1021 atgccggaat aaaagtaaat aaggacttga tcataaacag tgaatttgtc gaaaatgccg
     1081 gctatgaagc gaccaaaaaa ttattcaacg gaaaaagtaa gttttcggcc atttttgcca
     1141 gtaatgacaa aatagcattt ggtgcgatca aagccatcca agaagcaggg ctcaaagttc
     1201 cggaggatat ttctgtgata ggatttgatg acattgaatc atccaaatat tttaatccgc
     1261 cacttacctc gataaagatg gagttggtgg agatggcaga tctcgcaaca aagtcgattg
     1321 ttgcctctat tgaacaggat gtgaagcctt ctgtaaatat agtgcccgtc acattggtgg
     1381 aaagagagag ctgtgcactg ttgaaataaa ttggaacaac cggcatattt cccctgaatc
     1441 ggctgtcgtc agggctagga ttttacgggg aggcgatggt tttgctatga tagctttaac
     1501 aaaggatgag ggggtaagcg aagatggcgt acgaacctaa aaccaagccc acggaagtgt
     1561 ccgtggagaa gttcatcgag gaaatccagc agccgcaaaa gaaagaggat gcttacgcgc
     1621 tgttggcgtt gtttgcggaa gtgaccggag aagaagccaa aatgtggggg ccgagcatca
     1681 tcggattcgg gaaatatcat taccggtacg cgagcggcca tgaaggcgat gccccgctgg
     1741 ctgctttttc gccgcgcaag accgcgctga cgttttattt tatgctgccg gatggaaaac
     1801 gtgaggagtt acttgcgaag ctcggcaaac acaaaaccgg aaagggctgc gtgtacgtca
     1861 acaagctttc cgacattgat acagctgtgc tgaaggagat gatccgggaa gacatcgctc
     1921 atgcaaccca actttacgga ggagaggcag ctgacaaagc acttcctgct tctgcttcca
     1981 ttgcgaagcg gttgggattc gaaaaattcc aaaagcgtgc cgttctgggg aaagagcgcg
     2041 ccgtggcgga tgattttgcg gagttggatt cctatgatac ggatgtggat gccggaaaat
     2101 acgatttgat tttttcttac gtcctgacgt tggaggaact gaaggcacgc gtctgggata
     2161 cgatcaacca cgaccgcctg aatccggaag gctatcttta catcgcttat ccgaaaatcg
     2221 ggaacaaaag ctatgacaca tcggtacatc gcgatgccat ctttccgagt ctgggtgtgg
     2281 acgacggcaa aggtactgtc ggcgacagta ccttgaaatt tgcgcggctg gtcaagctgg
     2341 atgatacgtt cacgctcgtc gggatgaaaa atgctgtcaa aagtaaagac cacaagacga
     2401 agaatcttta ctagcagtag ctgaaggaac aagcataatc acaaaaacag gggactgccg
     2461 cgtgtggcag tcccctgttt ttggaagtag tttttttaag gatctccgat ctccgttgaa
     2521 cggaggcttc gccggagttc atacagattt cacggattct tctccggcga gcctttcttc
     2581 atcggaagtg acggcgtttc cgcggcacca gctc
//
LOCUS       DAOMUJ010000257         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11388_length_2597_cov_1.605035, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000257 DAOMUJ010000000
VERSION     DAOMUJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11388_length_2597_cov_1.605035"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLT73_06695"
     CDS             complement(<1..423)
                     /locus_tag="PLT73_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HRM20102.1"
                     /translation="MGKKHIALFASILLLSPIAGGFTAQAATLEELQQQKDALQLETN
                     TIQSQIEEKSNSLNTLESEKANLETKVNELQSQLDELMNRLATQEQKLADIESKILEL
                     QAEIEALQVVIDQRTEKLNTQARYIQTDAGVTDIASMLL"
     gene            672..851
                     /locus_tag="PLT73_06700"
     CDS             672..851
                     /locus_tag="PLT73_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HRM20103.1"
                     /translation="MRIDQKDLHKPGTDNLPPGVYKEVGPRGGKITGSKEVHIVEGHR
                     LPPTNKAGNKWIKKD"
     assembly_gap    1088..1097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1098..1405)
                     /locus_tag="PLT73_06705"
     CDS             complement(<1098..1405)
                     /locus_tag="PLT73_06705"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRM20104.1"
                     /translation="MTLKETVNKDFIQARKDKDTLKADVLRLIKTEYDSFIIDNKREM
                     TEAEEIHVFMKDIKKTNEAIAFAKQVERTDLIEENQKKLAILEAYVPKMMDEREVRFF
                     "
     gene            complement(1695..>2597)
                     /locus_tag="PLT73_06710"
     CDS             complement(1695..>2597)
                     /locus_tag="PLT73_06710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRM20105.1"
                     /translation="IPDFGFSPDAEFPIINGEKGMQTFMVQFRGDNKGGKNELLSFDA
                     GLRENMVPQDATAVFISPEADKIEQAFAAFLENNPVKGTISVAGEQVTIELIGKAAHG
                     MNPAAGVNAGTYLATFLNKFNFGGQAATFLELIADRIHLQHDAQKLNLAYTDSVMGEL
                     TMNAGIFTFTPSTGGELALNFRYPQGVSEEGIEIKLEAALATYGVTIAKGGHGKLPHY
                     VPADDPLVKTLLAVYEKHTGLKGHEQSIGGGTYGRLLERGVAYGAMFPDSIDTMHQAN
                     EFIALDDLFRATAIYADAMYELLK"
BASE COUNT          679 a          605 c          520 g          783 t
ORIGIN      
        1 caacagcatt gaagctatat ccgtaacgcc cgcatccgtt tggatataac gagcctgcgt
       61 atttaatttt tcagttctct ggtcgatgac cacttgtaat gcttcaattt cagcttgcaa
      121 ttccagaatt tttgattcga tatcagctaa tttttgctct tgagtcgcta accgattcat
      181 caattcatcc aattgcgatt gcagttcatt cacttttgtt tccaagtttg ctttttcgga
      241 ttccaaagta ttcaatgaat ttgatttttc ttctatctgc gattgaatcg tattcgtttc
      301 taattgcaaa gcatcttttt gttgttgtaa ttcttctaat gtagccgcct gagcagtaaa
      361 accacctgca atcggactaa gcaataagat tgatgcgaac aacgcgatat gtttttttcc
      421 caattatact tctcctcctg tttctactac gagatgaagt atatcaaaat taaaatacca
      481 ttgcatgata gaaatatgac aattgtgttt caatttgtta cgacagtcgt aaatcgtgaa
      541 atattgttca tgtttacaca tcggcttccg aacacaggct gcatttgatt gttcgcgctt
      601 ccagcgatat acttaagctg taccaagtgt ttcctccacc atcgctcttc aagaaagaaa
      661 ggggcaataa catgagaatc gatcaaaaag atttacacaa accaggtacg gacaaccttc
      721 ctcctggcgt ctacaaggaa gtcggcccaa gagggggcaa aattaccggc agcaaagagg
      781 tccatatcgt tgaagggcat agacttccgc ctaccaacaa agccggaaac aaatggatca
      841 aaaaagactg attgttcgct gataactgat catttgaaag cttcgcggtg gagaaaactt
      901 ggaaaggcgt tcagaaaagg accgtgcagc aagcgccgca cagtcctttt cttatccgat
      961 gcattatttt gccagaagtt cctcgatgat tttggatacg agtgctttat cggctttgcc
     1021 atccagcact tgcatcgaaa atttcatcgc atccttcaat gacatttcgg ctacaccgtt
     1081 ttcggccnnn nnnnnnnaaa aagaagcgaa cttcccgttc atccatcatc ttaggcacat
     1141 aagcctccaa aattgccaac tttttctgat tttcttcaat taggtccgtg cgttcaactt
     1201 gctttgcaaa agcgatcgct tcattggttt tttttatgtc cttcataaaa acatggattt
     1261 cctctgcttc agtcatttcg cgcttgttgt cgataatgaa agaatcgtat tccgttttga
     1321 tcaggcggag gacatctgcc ttcaacgtgt ctttgtcttt ccttgcttgg atgaagtctt
     1381 tgttgactgt ttctttcaat gtcatgatta ctgagctcct ctccgaaatt agttatagac
     1441 cgcccctcat tatggattct ttccaagatc agcgtcctcc cttaattcta aggaatagaa
     1501 tcggaattgg caaatctgta accattggcg tcgtttcatc attttctgaa aaattctact
     1561 ctatcgtatt tcatctactt gaaaacctat tatcgtgtta aggaagcatc atcagatgaa
     1621 tttcacaaaa aaacagaaat gctgatttaa cagcatttct gtttttggtg gtatgaaaca
     1681 atcagacagt ttgtttattt cagcaattcg tacatggcat ccgcataaat cgcggtcgcg
     1741 cggaaaagat catccaaagc gatgaattcg ttggcttggt gcatcgtgtc gatgctgtca
     1801 gggaacatcg ccccataagc aacaccacgc tccaacaagc gcccataagt tccgccgccg
     1861 atggactgtt catgtccctt taagccggtg tgtttctcgt agacagccaa caacgttttt
     1921 accaacggat cgtcagccgg cacgtaatgc ggcaattttc cgtgtccgcc ttttgcgatc
     1981 gtcacgccat aagtagccaa ggcagcttcc aacttaattt cgatgccttc ttcggaaacg
     2041 ccttgcggat aacggaagtt caacgccaat tcgccgccag ttgatggtgt aaaggtgaaa
     2101 atgccggcat tcatggtcaa ttcgcccatt actgaatccg tgtaagccaa atttagtttc
     2161 tgagcatcat gctgcagatg gatgcggtcc gcgatcaatt caaggaacgt ggccgcttgt
     2221 ccgccgaagt tgaacttgtt caagaaagtg gcaagatagg ttcccgcatt cacgccggca
     2281 gccggattca taccatgagc ggctttaccg atcaattcga ttgttacttg ttctccagct
     2341 acagaaatcg ttcctttgac tggattattt tctaggaaag cagcgaatgc ctgttcaatt
     2401 ttgtcggcct ccggactgat gaatacggca gtcgcatcct gagggaccat gttttcgcgc
     2461 agcccggcat caaaactcaa caactcgttc tttccgcctt tgttgtcgcc tctgaattgg
     2521 accatgaaag tctgcatgcc tttttcacca ttgatgatcg ggaactctgc atccggagaa
     2581 aagccgaaat caggtat
//
LOCUS       DAOMUJ010000258         2584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11473_length_2584_cov_2.250297, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000258 DAOMUJ010000000
VERSION     DAOMUJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11473_length_2584_cov_2.250297"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1532)
                     /gene="rpoN"
                     /locus_tag="PLT73_06715"
     CDS             complement(237..1532)
                     /gene="rpoN"
                     /locus_tag="PLT73_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRM20106.1"
                     /translation="MNLSQNQNLTQTQTQKQTLSQTMLQGIHILQLGNLELHDYLNQK
                     VLDNPFLQMKVRESRVSRSGSQANDLSWIPDRKQSLYEYITEQVMLTYRDTYLRTLIV
                     WWINQLNDKGYVIKSIEEAAAETKATPIQMMDALTLLQQLDPPGIGARNLQECLMLQT
                     ERREDAPDIAYIVLEESFDDLINRKWGAISKRYAVTLENIQSVFDFVQHLNPSPGAAF
                     QADEQISIRTDIIVTIKDAQLQINEARYGVPILSFNKAYAEELEQLKDKEVVKYVREK
                     KKEYESLQENLSLRSETILRVSTAIVHRQAAFFFDEAHPLVPLQLTDLAEELNLHEST
                     ISRAINDTYVQTDSGLYELKYFLSRKAKSGNEDAISTTSVQQMIQKLIEEEDKHKPLS
                     DQKIVDLLVQEKIDISRRTVAKYRTELNIPATSKRKRFD"
     gene            complement(1691..>2144)
                     /locus_tag="PLT73_06720"
     CDS             complement(1691..>2144)
                     /locus_tag="PLT73_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20107.1"
                     /translation="HRIVLMHKSIYSSADHARDEDVLAFRDGLVPVFDELDIDVALMG
                     HDHVYVRTYQMKGDAVVEEISYDEAGAAVDPEGTLYLTGNSASDSKYYDMQDSSVYER
                     YAAVALQLEEPTFMNVEVTDDSLTFTTYKTADQSVVDAYSIVKVDSGE"
     assembly_gap    2145..2154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2155..>2584)
                     /locus_tag="PLT73_06725"
     CDS             complement(<2155..>2584)
                     /locus_tag="PLT73_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM20108.1"
                     /translation="ATEDGDFNFLFFGDPQIGAGDGTQIDADGWDKTVTVATEMFPDT
                     DFLVSAGDQVNIANNLNQYDGYFFPEELTFYPIMTLIGNHDYTPFADYFNPPNETGLG
                     ADDAGSDFYFTYGNTLFIALNTQRNNIEEHREAMELAIAEN"
BASE COUNT          625 a          635 c          589 g          725 t
ORIGIN      
        1 atcgttgcac tggaagcagc catccaagac taatgatcgg tgttaaaaaa cttcttccat
       61 agaaaaacgt ggggctgtct caaatgaggc ggcctctttg cgtacgccgg ctgaaggaac
      121 gtatgaataa agctaaaaaa acgagggaac gtctcattga atgatgagac accctcgttt
      181 tttgttgcgt attatcaaaa atataaaaat gccagccgat tattgtgcct ttcggatcag
      241 tcgaaccgtt tcctcttcga agtggctggg atgttgagct ccgtcctgta cttggccact
      301 gtccgcctcg agatatcgat tttctcttgg accaacagat cgactatttt ctgatcggac
      361 agcggtttgt gcttgtcctc ttcctcaatc agtttttgga tcatctgttg gaccgacgtt
      421 gtggagatgg cgtcttcatt tcccgatttg gctttccggg acaagaagta cttcagttca
      481 tagagacccg aatctgtttg gacatacgtg tcgtttattg cgcgactgat ggtggattcg
      541 tgcagattca attcctcggc gaggtctgtt aattgcagtg gaacaagcgg atgcgcttca
      601 tcaaagaaaa aagctgcctg tctgtgcacg attgccgtac tgacgcgtaa aatcgtttca
      661 ctgcggagcg acaaattttc ctgcagcgat tcgtactctt tctttttttc cctgacatac
      721 ttaacgactt ccttgtcctt gagttgctcc agttcctctg cgtaggcttt attgaaggaa
      781 aggatcggca cgccgtaacg ggcttcgttt atctgaagtt gtgcatcctt gatggtgaca
      841 atgatatccg ttctgatcga gatttgctca tcggcttgga atgcagcccc gggagaagga
      901 ttcagatgct ggacaaaatc gaaaacggat tggatatttt ctaatgttac cgcatatctt
      961 ttagaaatgg cgccccattt tctgttgatc aaatcatcaa atgactcttc caacacgata
     1021 taagcgatat cgggtgcatc ctcccgccgt tccgtctgca gcatcagaca ctcctgcagg
     1081 ttacgcgccc cgatgccagg cggatcgagt tgctgcagca gtgtcaaggc atccatcatt
     1141 tggatcgggg tggccttcgt ttcagcagca gcttcttcga tgcttttgat gacatacccc
     1201 ttatcgttca attggttaat ccaccaaaca atcaaggtgc ggaggtaggt atcccgataa
     1261 gtcagcataa cttgctccgt aatatattca taaagggatt gtttacggtc aggtatccaa
     1321 ctcaaatcat tggcttgact gccgcttctg gaaacccgcg actcccttac tttcatctgc
     1381 agaaacggat tatccaaaac cttctgattc agataatcgt gcagttcgag gtttcctaac
     1441 tgaagtatat ggattccttg cagcatcgtt tgggaaagcg tttgcttttg tgtctgcgtt
     1501 tgggtaagat tttggttttg gcttaagttc acacatattc ctcttttctg cttcaactct
     1561 gatttgatga tgcacgcact gacactatac catttttcgt taaaaaatgg ctttattttg
     1621 gcgcaaaaaa aacgttaccc tcgagaaagc tcatccgaag gcaacgaaaa gactatccag
     1681 cttttctctt tcattccccc gaatccacct tcacaatgct gtaagcatcc acaaccgact
     1741 gatcggcggt tttataggtt gtgaaagtta gggaatcgtc ggtcacttcg acattcataa
     1801 aagtcggttc ctccaactgc agcgcaacgg cggcatagcg ttcatagacg gaggaatcct
     1861 gcatgtcgta atatttggag tcagaagctg agttaccggt caaatacagt gtgccttccg
     1921 gatcgacagc ggcacctgcc tcatcgtagg agatttcctc aacgaccgca tcgcctttca
     1981 tctgataggt tcgcacatag acatggtcat gccccatcaa agccacatcg atatcaagtt
     2041 catcgaagac cggcaccaat ccgtcccgga aagccaacac atcctcatcg cgcgcatgat
     2101 cggcactgct gtagatggat ttatgcatca gcacaattcg atgcnnnnnn nnnngttttc
     2161 cgctatagcc aattccattg cttcccgatg ctcttcgata ttgtttcttt gggtgttcaa
     2221 ggcgataaac agcgtgttcc cataggtaaa ataaaagtca ctgccggcat catccgcgcc
     2281 cagccccgtc tcgttgggcg gattgaaata atcggcaaaa ggcgtgtaat catggttgcc
     2341 gattagggtc atgatcggat agaaggtcag ttcttccgga aagaagtagc cgtcatattg
     2401 gttcaaattg ttggcgatat tcacctgatc acccgccgat acaaggaaat ccgtatcagg
     2461 aaacatctcc gtcgcgaccg tcactgtctt gtcccatcca tccgcatcga tctgcgtgcc
     2521 gtcgccggcc ccaatctgcg gatcgccgaa gaacaggaag ttgaagtcac cgtcctcggt
     2581 cgcc
//
LOCUS       DAOMUJ010000259         2581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11480_length_2581_cov_2.440222, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000259 DAOMUJ010000000
VERSION     DAOMUJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11480_length_2581_cov_2.440222"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..979
                     /locus_tag="PLT73_06730"
     CDS             287..979
                     /locus_tag="PLT73_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM20109.1"
                     /translation="MDEDSNVKSYAAPNVAIMYGTNINDEISKRTNKKVASINDAKAA
                     GLCEFELGNAKGYKSSAFLIIGTGTGGCICDENGVIYGKDAFAGEFHNMPFMNAKIGG
                     LDKMGDYASITGLVNLYNEKAAEYDKVQYGYEVSHKYLNGDRIAVSSVEEWIRNIVAQ
                     LLVITVFYNPEVICIGGGISEEDWFIDKVSAQYKKTCIDYFEADFITTEIDRCKYNND
                     ANLLGAVIKASL"
     gene            1049..2488
                     /locus_tag="PLT73_06735"
     CDS             1049..2488
                     /locus_tag="PLT73_06735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM20110.1"
                     /translation="MIYEKLDTFRDDFLWGSASAAYQVEGAWDQDGKGESVWDVYTKL
                     PGTTFKNTNGDVAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPDGKSEVNEEGLK
                     FYENLIDELIANGIKPVVTLYHWDLPQHLQDLYGGWESREIIQDFNRYCVTLFKRFGS
                     KVKHWVSLNEQNIFMSLGYVTAMHPPAVKDQKRMLQANHIANLANATVIESFKTYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGL
                     APVIEEGDLELLERVKPDFIGINYYQTSTIAFNPLDGVGQSEGINNTGKKGSAKESGL
                     PGVYKNAKNPHLETTNWDWAIDPTGLRVGLRRLTSKYNLPILITENGLGEFDKLEEND
                     VVADVHRIDYLSAHVKACKEAVTDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYIDRD
                     ENDQKDLRRIKKNSFYWYKNVISSNGEEL"
BASE COUNT          846 a          442 c          633 g          660 t
ORIGIN      
        1 aaccaaacaa taaacgcgcg tttacaaagt tgaagtgaga tgaagattca gtaagtaatc
       61 tgcaaaacct tcattttaag gagaagtgtt atggggaaaa gtcatttagg aatagatata
      121 ggagggacat tcatcaagta tgcattaatg gataagtatt attgcatcaa agacaagtgg
      181 aaaatcgata cgattaagta cgatacggca gcggagtgat tacatctgct caaacataag
      241 atcaattgat gaaatcgaca aaattggtgt aagcgcaccc ggtcttattg atgaagactc
      301 aaatgtgaag tcatatgctg ctccaaacgt agctattatg tatggcacaa acatcaacga
      361 cgaaatcagc aaaagaacaa acaaaaaggt agcctcaatc aacgatgcaa aagccgcagg
      421 attatgcgag tttgaattgg gaaatgcaaa agggtataag tcttctgcgt ttttgattat
      481 aggaacagga acaggcggct gcatctgtga tgagaatggc gtcatttatg ggaaagatgc
      541 ctttgcggga gaatttcaca acatgccttt tatgaacgcc aagataggtg gccttgataa
      601 aatgggcgat tatgcgtcga tcactggcct tgtaaatctg tataacgaaa aagccgctga
      661 gtatgacaag gttcagtatg gctatgaagt atcccataag tatttgaatg gagacaggat
      721 agcggtgtca tccgtggagg aatggataag gaacatagtg gctcagctgc tggtgatcac
      781 cgttttctat aatccggaag tgatctgcat cgggggagga atttcagaag aggattggtt
      841 tatcgacaaa gtaagcgcgc agtacaagaa aacctgcatt gactattttg aggcggattt
      901 catcactacg gaaattgata gatgcaagta caacaatgat gccaatttgc tgggtgctgt
      961 cattaaggct tccctttgag ctaaaaagca gtgttttcaa gaaaagtcat cagaaatcaa
     1021 atttttaaga acaggaggag aggctgcagt gatatacgaa aaattagata cattccgtga
     1081 tgatttccta tggggatcgg catcggcagc atatcaagtt gagggtgcat gggatcaaga
     1141 tggaaagggt gaaagtgtat gggatgtgta cacgaaatta cccggcacaa cttttaaaaa
     1201 tacaaatggg gatgttgcgg tagatcatta caaccgatac aaagaagacg ttgccttgat
     1261 ggctgaaatg ggattgaaag cctatagatt ttcaattgca tggacccgca tttatcctga
     1321 tgggaagagt gaagtgaatg aggaaggctt gaaattctat gagaatttga ttgatgaact
     1381 cattgcaaac ggtatcaaac ctgtcgtcac tttgtatcac tgggacttgc cacagcacct
     1441 gcaggatctg tatggtggct gggaatcaag agaaatcatt caggatttta acagatattg
     1501 tgtaaccttg tttaagcgtt ttggcagcaa agtcaagcat tgggtttctt tgaatgaaca
     1561 aaatatattt atgtcgctgg gttatgtaac cgctatgcac ccgccggctg taaaagatca
     1621 gaagagaatg ctgcaggcga atcatattgc aaacttagct aacgctacag taatcgaatc
     1681 attcaaaacc tacgttccgg acggcatgat tggaccgagt tttgcatttg gacccgtgta
     1741 tccgttcagc tgcgatccga aagatgtttt atctgcagaa aatgcggaag atcttaattg
     1801 caactggtgg ctggatgtct actgcaaagg aaaatatcca gtgttcgcct tcaaatatta
     1861 tgaaagactt ggactcgcac ctgttatcga agaaggtgat ctcgaacttt tggagagagt
     1921 caaaccggat tttatcggca tcaactatta ccaaacaagc actatcgcgt tcaatccgct
     1981 tgatggagta ggacaatcgg aagggatcaa caacacaggc aaaaaaggga gcgctaaaga
     2041 aagcggattg cccggcgttt ataagaatgc gaaaaatccg catctggaaa cgaccaactg
     2101 ggattgggcc atcgatccta cgggattaag ggttggatta agaagattaa ccagcaagta
     2161 taatcttccg atactcatta cagaaaatgg tctaggggaa tttgataagc tcgaagagaa
     2221 tgatgttgtg gctgatgttc acagaatcga ttatctcagt gcccatgtca aagcttgcaa
     2281 agaagcagtc acagatggtg tcgatcttct gggatactgc acctggtcat tcacagattt
     2341 gttgagttgg ctcaacggtt atcagaaacg ttacggattc gtctacatag acagggacga
     2401 aaatgatcag aaggatctga gacgtatcaa aaagaacagc ttctattggt acaagaatgt
     2461 catcagcagc aacggagaag agctataaat ttcgtccata agcagtgaat acaaaaataa
     2521 ggagtgagga atcatgaaaa aaattttact agtatgttca gcaggtatgt ccacaagttt
     2581 g
//
LOCUS       DAOMUJ010000260         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11486_length_2579_cov_2.346672, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000260 DAOMUJ010000000
VERSION     DAOMUJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11486_length_2579_cov_2.346672"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    191..608
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            692..2122
                     /gene="trpE"
                     /locus_tag="PLT73_06740"
     CDS             692..2122
                     /gene="trpE"
                     /locus_tag="PLT73_06740"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRM20111.1"
                     /translation="MFNLTATEFAAAKKHNKVFPVHLTVNANQLTPIGMYYNLEGEHK
                     FLFESVFSEREIGRYSFLGSNPYKSITSMGEDVTIITDGQPVHKKGKVLDYVKEQVSI
                     PYLKTDIPLPFVGGAIGYVGYDVIRQYEKLPNVNPDELQAPEAYLMFYKQFISYDHYQ
                     HKMTLVHNVFPEDTEDYETITANLEKLAETMNQTNGIRPFQEVNPNQEVSSNVSEAEF
                     CKLVEKAKEYIKAGDIFQVVLSQRLTIKSESEPFDIYRRLRTKNPSPYLFYIDLGDFQ
                     VVGSSPESLVTVQDGEVMTNPIAGTRRRGKNEEEDNELKKELLADEKERAEHVMLVDL
                     GRNDIGRISEFGSVKLDKFMEVDIYSHVMHIVSKVTGKLKPGLTCFEALSACLPVGTV
                     SGAPKIRAMGIIDELEKTKRGIYAGAVGYFSYNGNMDTCIAIRTIILKDGFAYVQAGA
                     GIVHDSVPQSEYTETLNKAMALKEVI"
     gene            2123..>2579
                     /locus_tag="PLT73_06745"
     CDS             2123..>2579
                     /locus_tag="PLT73_06745"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRM20112.1"
                     /translation="MILLIDNYDSFTYNLYQYIGEINPDVQVLRNDALTLDEIKGMDL
                     DCIILSPGPGVPEDAGICVDVVREFAGKVPLLGICLGHQAIGYAYGGNVIRAETLKHG
                     KTSMITHNGDPLFQGIASPFEVMRYHSLVVEEKSLPDELKILSKSVDDGV"
BASE COUNT          733 a          551 c          676 g          619 t
ORIGIN      
        1 ccagtgcccg cgagggcgtg ccggttcgac cccggccgcc ggtatcacaa aagaagctgt
       61 tcataacaag gagtactttg ttatgaacgg cttctttttc tattgcctgg gctcagtggt
      121 ttttcgaaag acaaatattt ttcagttgac aataatgaga aaaggattat aatagattaa
      181 aattaaatta ataaaatctg tgatgagaag tagtaaccaa tggcgtttag tcaaagcgag
      241 tttggggcag tgaaagccaa gcactgtagc tgtcggtgaa tggatctctg agacgtgatg
      301 cgaaaattat agtagcaaaa accgggagtc cgccgttaaa aggacggggt atcgagtaac
      361 atctgtaccc gcagagcgag ttttgtactg aggtggcaaa cgaatggaaa catttcgtcc
      421 ttttgaggag gaagtgtttt tttgattctg tagtattagc gcgaaaacaa tgcatagccc
      481 attttttggt gacgcattgg gtccttgatt ccgataagga ggcaaggagc gcaaagaacg
      541 acagtgtcgg gtgtgagtca acagtagaga actaatatgg gtggcaccgc ggctacatcg
      601 tcccattata ggggatgttg tggctttttt tgtacccaaa atcccctgaa tcaagcagaa
      661 acagcaaaaa atctcattag gggtgaatgg aatgtttaat ctgacagcaa cagaatttgc
      721 agcagcaaaa aagcataata aggtgtttcc ggtacacttg acggtcaatg cgaatcaatt
      781 gacgcctatc ggcatgtact acaatctcga aggggagcat aagtttttgt ttgagagtgt
      841 cttttcggag cgggaaatcg ggagatactc cttcttgggt tcgaaccctt ataagagcat
      901 cacgagtatg ggcgaagacg tcacgatcat caccgatggg caacccgtgc acaaaaaagg
      961 caaggtgctc gattacgtaa aagagcaggt gtcgattccg tatctcaaga ccgatatccc
     1021 gttgcctttt gtcggcggcg cgatcggtta tgtcggttac gatgtcatcc gacaatacga
     1081 aaagctgccg aatgtgaacc cggatgaact gcaggcgcct gaagcctact tgatgttcta
     1141 caagcaattc atcagctacg accactacca acacaaaatg acgcttgtcc ataatgtttt
     1201 ccccgaggac acggaggatt atgaaacaat cactgccaat ctggaaaagt tagccgaaac
     1261 gatgaaccag acgaatggca tccgaccttt ccaagaggtg aatccgaatc aagaagtttc
     1321 ttcaaatgtc agcgaagcgg aattttgtaa actcgtcgag aaagcaaaag aatacatcaa
     1381 agcgggcgac atctttcagg ttgtcctttc gcagcgcttg actatcaaat cggaatccga
     1441 accgttcgac atctacagaa gactgcgcac aaagaatccg tcgccttacc ttttctatat
     1501 cgatctgggt gatttccagg tggtcggttc ctctccggaa agtctggtca ccgttcagga
     1561 tggcgaagtc atgacgaatc cgatagccgg aacacgcaga agagggaaga acgaagagga
     1621 agacaatgaa ttgaagaaag agttgcttgc ggatgagaag gagcgggcag aacatgtgat
     1681 gctggtggat ttgggaagga acgacatcgg caggattagc gaattcggca gcgtcaagct
     1741 ggataagttc atggaagtgg atatttattc acacgttatg cacatcgttt cgaaggtgac
     1801 cggcaaattg aaaccgggtc tgacctgctt tgaagccttg agcgcgtgtt tgcctgtagg
     1861 gaccgtatca ggtgcgccga aaataagggc gatgggcatc atcgacgaat tggaaaaaac
     1921 gaagcggggc atctatgccg gtgcggtcgg ctatttttcc tataatggca acatggacac
     1981 ctgcatcgcc atccggacca tcattctgaa ggacggcttc gcctatgttc aagccggcgc
     2041 agggattgtg cacgattccg ttccgcaatc ggaatatacc gagacactca acaaagcaat
     2101 ggccttgaag gaggtaatct gaatgatttt attgatcgat aactatgatt cctttacgta
     2161 caacctctat caatacatcg gagaaatcaa tccggatgtg caggttctgc gcaatgatgc
     2221 cctgacactt gatgaaataa aagggatgga cctggactgc atcatccttt ccccaggacc
     2281 gggcgtgccg gaagatgccg ggatctgcgt tgatgtggtc cgggagtttg ccggaaaagt
     2341 acctttgcta ggaatctgtc ttgggcatca ggccatcggg tatgcttatg gcgggaatgt
     2401 cattcgtgca gaaacgctga agcacggtaa aacctccatg atcacacata acggagaccc
     2461 tcttttccaa gggatcgctt ccccattcga agtcatgcgt taccattcgc tggtagtcga
     2521 ggaaaagagc cttcccgacg aactgaaaat actctccaaa agtgtggatg atggcgtca
//
LOCUS       DAOMUJ010000261         2578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11494_length_2578_cov_2.228300, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000261 DAOMUJ010000000
VERSION     DAOMUJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11494_length_2578_cov_2.228300"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1383
                     /locus_tag="PLT73_06750"
     CDS             277..1383
                     /locus_tag="PLT73_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20113.1"
                     /translation="MELFDQLMNPNWKNIPMEHKLVLASSVVRYFVNPLLPVANIQPV
                     YYQMGGIKTETFTVDINGAAFVFVPGHKEAVLGWDSGINGLDPLDISSDRKEMYLAIE
                     NCLKLSVQGAEFLHDDHWMKKKIGPIHNLEELEDLVNSQMSQLRTTEIPPLLVEVSPN
                     YVGMTKVGDYAVINGKFEGQSDWFSIHEKDIHQALMPSATGSSVFADFPAHLVQNDSF
                     FLRQNEDLDSYTVFAPCQTTYAEIRKQLEKDGMGLLSSNEWEYCCGASTRRLFRWGNQ
                     LHRNLFEPEDSHLYKKNMFGLEIANMGWGPELVEDGLLVKGGWISIQAENILEKLLPY
                     ATYYENHADALNEKQQEPLAPGYYCARRSIRIEM"
     gene            1707..>2578
                     /locus_tag="PLT73_06755"
     CDS             1707..>2578
                     /locus_tag="PLT73_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRM20114.1"
                     /translation="MKNLTSSDIRQMYLDFWATKGSKVEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGTLIPENPRITNAQKSIRTNDIENVGVTARHHTLFEMLGNFSIGDYFKE
                     EAIPWAWEFLTDEKWLAFDPELLYVTYYPEDTDTKRIWQEKVGLPEDHIVPIADNFWD
                     IGAGPCGPDTEIFYDRGPSFQDLPDGDPEMYPGGENERYLEIWNLVFSEFNHKPDGTY
                     EPLPHKNVDTGMGLERVTSVVQNTPTNFETDLFMPIIKKIETLSDGKKYGENKTLDIS
                     FKVIADHIRAVSFA"
BASE COUNT          764 a          534 c          638 g          642 t
ORIGIN      
        1 gagcatcgat ccgatgatcg gcatgggaga tgtaaggccg gaaactttca tgagtgaaaa
       61 ataatccggg aaaagtccgc agacaatgaa taatttgtct gcggactttt cagctgtcag
      121 aaaatcaatc agcgcagaat gctccgatca aagtcgcatc caaattcggt aggctcagca
      181 aaagaaataa gctactcgcc ggatcaatat tctgctatac tgacagtaaa gcatgttact
      241 ggttttccaa tacagctaca agaagagagt ggtataatgg aactatttga tcaattgatg
      301 aatccgaact ggaagaacat tccgatggag cacaagcttg ttttggccag cagtgtcgtc
      361 cgttattttg tgaaccccct gttgcctgtc gcgaatatcc agccggtgta ttatcaaatg
      421 ggcggaatca agaccgagac atttacagtg gacatcaacg gagcggcgtt tgtttttgtg
      481 cctgggcaca aggaggcagt cctcggatgg gacagcggaa tcaatggcct ggacccgttg
      541 gatatcagct ccgacaggaa agagatgtac cttgccatcg aaaattgtct taagctttcg
      601 gttcaaggcg ctgagtttct gcatgacgat cattggatga agaagaaaat cggaccaatt
      661 cataatttgg aagaattgga agatttggtc aacagtcaaa tgtctcaact ccggacaaca
      721 gaaattccgc ctttattggt ggaagtcagt ccgaattatg tgggcatgac gaaagtcggg
      781 gattatgcag tcataaatgg gaaatttgaa ggccagagcg attggttcag tatacacgaa
      841 aaggacattc atcaagcttt gatgccgagc gcaactggat cttcggtatt tgcggatttt
      901 ccggcgcatc tggttcaaaa tgactccttc ttcctgagac agaacgaaga tttagattca
      961 tacacagttt tcgcgccttg ccagactacc tatgcagaga tacgcaagca attggagaag
     1021 gatggcatgg gacttttatc cagcaatgag tgggaatatt gctgcggggc atcgacccgc
     1081 agacttttcc gttggggcaa tcagttgcat cgcaacctgt ttgaaccgga agacagtcat
     1141 ctctacaaaa agaacatgtt tggtctggaa attgcgaaca tgggttgggg cccggaactt
     1201 gtcgaagacg ggttgttggt aaagggtggt tggatatcca tccaagccga gaatatattg
     1261 gagaaactcc tgccgtatgc tacttattat gaaaaccatg cggatgcttt aaacgagaag
     1321 cagcaagagc ctttggcacc cggatattac tgtgcaagac gttccatccg catagaaatg
     1381 taacctgttc cgcttttctg aagaacagct tgcatgcggc tgtaattttg gttatactag
     1441 tttttgattg aagcagagga tatacaaatg gataacaaaa gagagtttcc gcttggtgaa
     1501 aggaaacgca tccgtttatg ctccctttgc gtgaattata gtagaaatac tatcgcttcc
     1561 agcgttaacg gatccgagag gtaatgaatg atcaaaacat ttcattgcaa acaaggtggt
     1621 accgcgtgat tcgtccttgt atgcagtggg atgtttttta tttttgttca ctcaaaaaaa
     1681 ataatgaaga gaaaaggaag gtaacaatga aaaatctgac aagcagtgac atccgtcaaa
     1741 tgtatcttga tttttgggca acaaaagggt cgaaagtgga accgagtgct tcgctgatac
     1801 cggtcaacga ccctacgcta ttatggatca actcaggagt agcgacattg aaaaagtatt
     1861 ttgatggcac attgattcct gaaaatccgc gcatcaccaa tgcccaaaag agcatccgta
     1921 caaacgacat cgaaaatgtc ggggtgaccg caagacacca tactttattt gaaatgttgg
     1981 ggaatttctc gatcggggac tacttcaaag aagaagcaat cccttgggct tgggaattcc
     2041 tgactgacga aaaatggttg gcgttcgatc cagaactgct ttatgtcact tactatcctg
     2101 aagacacaga tacgaaacgc atttggcagg aaaaagtcgg tttgccggaa gatcatatcg
     2161 ttccgatcgc ggacaatttt tgggatatcg gggcaggacc atgcggaccg gatacggaaa
     2221 ttttctacga ccgcggacca tccttccagg atttgcctga cggggatcct gaaatgtatc
     2281 cgggcggaga aaatgaacgc taccttgaaa tctggaacct tgtgttctct gaattcaacc
     2341 acaaaccgga cggcacctat gagccgttac cgcacaagaa cgtcgatacg gggatgggtc
     2401 tggagcgcgt cacgagcgta gtccaaaaca caccaaccaa ctttgaaacg gatctgttca
     2461 tgccaatcat aaaaaaaatc gagacgttga gcgacggcaa gaagtacggc gaaaacaaaa
     2521 cattggatat ctctttcaaa gtcatcgccg atcatatccg cgccgtcagt tttgcgat
//
LOCUS       DAOMUJ010000262         2575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11515_length_2575_cov_1.840476, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000262 DAOMUJ010000000
VERSION     DAOMUJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11515_length_2575_cov_1.840476"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLT73_06760"
     CDS             <1..1312
                     /locus_tag="PLT73_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM20115.1"
                     /translation="ALPAERKLAEELQVNRSTVIRALEELTTQGILIRKRGSGTFVNP
                     NKWGMQTRPTINWRTSLIPDNLSVDTPYQRNVKQLLSQHKTREILDLGNGDLPKDLLP
                     ELKIPDISLGEMLLGEATFTKENRGIKATRESVQKHLQTAYGMTVPLEEIFITSGTQQ
                     SLFLITQGLLKPGDAIGIEAPSYFYSLRLFQAAGLRIIPIPMDDEGMTVKGLQETSLQ
                     YPLKMIFLNPIFQNPTGAVMSPERKQAILDYCLLKRIPIVEDDAYGSFVFDESVDNRP
                     LKSLDKRQQVLYLGSLSKFAGQHIRIGWMVGPAAIVRELADIRDQIDSGLSFLPQLLA
                     EHYLASEITEHLPIIVTALKERADKLQTWLRTKYGPEISFEPVKGGYHLYCHFANKTD
                     AEMDELLQELLEEKVIVKEGIQFGDKKNAVRFSFGHFLERIAKI"
     gene            1426..2439
                     /locus_tag="PLT73_06765"
     CDS             1426..2439
                     /locus_tag="PLT73_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20116.1"
                     /translation="MSTILDVAEKAKVSIATVSRALNNQPGCSEETRKKVKKIAQELG
                     YEPNEVARSLINKKTNTIGVIIPEISSLLSSEFINGIDAIAKQESSNLIVTYTKSNIV
                     STEENLKMLHEKRVDGIIFVSEILRKEYYDYIKRNNIPCVLLSTKSAEYASVPYVKVD
                     DYDASYAATNYLIKMGHKKIGLISGSSADPIAGNPRIDGYKKAILENLNKVVEERQIV
                     SGNGFGFDDGRDNFIQLMKQFPELTAIFCVSDEMAIGAISAAYRLGIKIPEDVSIMGY
                     DNLKISEMSVPPLTTTAQPLVKMAEKATEILFKIINKKADDVHSIIMPYQIIERESVK
                     NIK"
BASE COUNT          812 a          492 c          618 g          653 t
ORIGIN      
        1 agctttaccg gcggaacgca agttggcgga ggaactgcag gtcaaccggt cgaccgtcat
       61 ccgggcactg gaagagctga cgacgcaagg gatccttatc cgcaaaagag gcagcgggac
      121 gttcgtgaat ccgaataaat gggggatgca gacaaggcca accatcaatt ggcggacttc
      181 cttgatcccg gataatcttt cggtggacac cccttatcaa aggaacgtca agcagctatt
      241 gtcacagcac aagacgcgcg aaattctgga tttggggaat ggggatttgc cgaaggacct
      301 cttgccggaa ctgaagatcc cggatatttc gctaggggaa atgttgctcg gggaagcgac
      361 cttcaccaag gaaaaccgcg gcatcaaggc aacgagagaa agcgtccaaa aacacctgca
      421 gactgcgtac gggatgactg tcccgctgga ggaaatcttc atcacttcgg gaacccagca
      481 atccttattt ttgattacgc aagggctgct gaaaccgggc gatgcgatcg gtatcgaagc
      541 gccttcctat ttttattctt tgcgattatt ccaggctgcc ggtctgagga tcattccgat
      601 tccgatggac gacgagggaa tgaccgttaa gggtctccaa gaaacttctt tgcagtatcc
      661 tctcaagatg atttttctga atccgatttt ccagaatccg acaggagcgg tcatgagtcc
      721 tgagcggaaa caggccatcc ttgattattg tttgctgaag cgcatcccga tcgtggagga
      781 tgatgcatac ggaagctttg tttttgatga atccgtcgat aaccgtccgt tgaaaagctt
      841 ggataaaagg caacaagtgc tgtatctggg ttccttgtcc aaatttgccg gccagcacat
      901 ccggatcggt tggatggttg gaccagccgc cattgtccgg gaattggccg acatccgcga
      961 tcagatcgac tccggattga gtttcttgcc gcagctgttg gccgaacatt atttggcgag
     1021 cgagatcact gaacatcttc cgatcatcgt tacggctttg aaagagcgcg ccgacaaatt
     1081 gcagacgtgg ctaagaacca aatatgggcc agaaataagt tttgagccgg taaaaggcgg
     1141 ctaccatctt tattgtcatt tcgccaataa aaccgatgcc gagatggatg aactgctgca
     1201 ggaattgttg gaagaaaaag tgatcgtgaa ggaaggtatc cagttcggcg acaaaaaaaa
     1261 tgctgtccgt ttcagtttcg gtcatttttt agagcggatt gcaaagatat gagtcaggaa
     1321 gcaaatctgc tgactagtct atttgataat aaaaaatacg tttacaaaat aaataacaca
     1381 tgatatatta ttccacgtaa acgtttacga aataggggat gctttgtgag tacaatacta
     1441 gatgttgcag aaaaagcaaa agtttctatc gccacagtct ccagagcctt aaataatcag
     1501 cctggatgtt cagaagaaac gagaaaaaaa gttaagaaaa tagctcaaga attgggttat
     1561 gaaccaaacg aagtcgccag aagcctaata aataaaaaaa cgaatacgat aggtgttatc
     1621 atacctgaga tttcaagtct gttaagttca gaatttatta atggaattga tgctatagcc
     1681 aagcaagaaa gttcaaactt aattgtcacc tacacgaaat cgaatattgt aagcacagaa
     1741 gagaatctaa agatgcttca cgagaagcga gtggatggca ttatattcgt cagtgaaatc
     1801 ctgcgaaagg aatattatga ctatataaaa agaaataata tcccttgtgt attgctgtct
     1861 acgaaatcag ctgaatatgc gagtgtccca tatgtaaaag ttgatgatta tgatgcatct
     1921 tatgcagcca ccaattattt gatcaagatg gggcataaaa aaattggttt aataagcgga
     1981 agctctgctg atccaattgc ggggaatcca cgaattgacg gttacaaaaa agcaatatta
     2041 gaaaatctta acaaggttgt cgaagagaga caaattgtat caggaaacgg gttcggattc
     2101 gatgatggaa gagataactt cattcaatta atgaagcaat ttcctgaatt gacagcgata
     2161 ttttgtgtga gtgatgaaat ggctatcgga gcgatttctg ctgcgtacag attaggtatc
     2221 aaaataccgg aagatgtctc tattatgggc tatgataatt tgaaaatatc cgagatgtca
     2281 gtgccgccgc tgacaacaac tgctcaacca ttggtgaaaa tggcagagaa ggcaactgaa
     2341 atattattca aaattataaa taagaaggct gacgatgtgc attcgattat aatgccatat
     2401 caaatcattg aaagagaaag tgtaaaaaat ataaagtaac aaatttcccg attgacgtaa
     2461 aagtttacct ggaacgacac gggaaattta gtttttgacc gtaacgtaaa cgtttacgta
     2521 gcgaagcgtg ttgtttttgt ttagtatcaa cgtaaacgtt tacgttgcga agaaa
//
LOCUS       DAOMUJ010000263         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11609_length_2558_cov_1.496205, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000263 DAOMUJ010000000
VERSION     DAOMUJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11609_length_2558_cov_1.496205"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..517
                     /locus_tag="PLT73_06770"
     CDS             107..517
                     /locus_tag="PLT73_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRM20117.1"
                     /translation="MSEEISLSMDADGVYMNVKAAILFAPGSAVITESGRPAVQKVAE
                     LISNFENKVVIEGHTDNIPHHDQKFDSNWELSAGRAIAVLRHLVEQRNIDPSRLSAVG
                     YGEYNPLVPNDTAEQRAANRRVNIVLVYEPEGAE"
     gene            517..990
                     /locus_tag="PLT73_06775"
     CDS             517..990
                     /locus_tag="PLT73_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRM20118.1"
                     /translation="MEKEAQLPKKQSIPKLVLIAVILMVLMVGGGMIGSLMTNGLATE
                     WIANFNNKDAESILVPQEEFLVNLKSNDGSLDDFLKIEMSVETTDEDNGAILTEKAAI
                     VRDAIISVLRNKSTANIFDESEGVLVIKQEIISKINQSLGSEIASDLYITNIVMQ"
     gene            1017..>1292
                     /locus_tag="PLT73_06780"
     CDS             1017..>1292
                     /locus_tag="PLT73_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRM20119.1"
                     /translation="MENELLYIGKSFIVLIFVIFAINYLLKLLDRFVNKNGKYITMIE
                     RVPLNRTSSLCIVKVSESYYLMSCTDTKNEILKELNIEDIKEEKTVLD"
     assembly_gap    1293..1302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1388..2122
                     /gene="fliP"
                     /locus_tag="PLT73_06785"
     CDS             1388..2122
                     /gene="fliP"
                     /locus_tag="PLT73_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711582.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRM20120.1"
                     /translation="MVKKGIVTSGVGLLFLTFPQKVHAAEIVDTVQNALGGTSDAVKM
                     YVLIGLLSVIPAFLILTTCFTRIIIVFSFLRSSLGTQQNPPNTVLIGLAAFLTFFIMR
                     PAYTEVYHEAAVPLMEDQIGIEEFFYIAEDPIRDFMLKQTREKDLTLFLDLQGDDMPT
                     NTGDVSLLSLIPAFAISELRTAFTIGFLIFIPFLVIDIVVASILMSMGMFMLSPVMIS
                     LPFKLLLFVLVDGWYLVVESLVLGFQ"
     gene            2133..2396
                     /gene="fliQ"
                     /locus_tag="PLT73_06790"
     CDS             2133..2396
                     /gene="fliQ"
                     /locus_tag="PLT73_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRM20121.1"
                     /translation="MSIEKVLDVLREGFMTILIVAGPTLAIALVVGLIISIIQAATQL
                     QEQTLSFVPKILAVIGALVVFGNFMLNALIAYTEKIFELIAGL"
BASE COUNT          713 a          446 c          640 g          749 t
ORIGIN      
        1 aacaaaggct ttaattgcta cgacctcttc attactgtaa acaaaatcat tttctggatc
       61 tgtaataatt aaaacattga ttccagtttt tgttgaaaaa aatggattgt ccgaagaaat
      121 cagtttgagt atggatgctg atggtgtcta catgaacgtg aaggcagcaa tcttgtttgc
      181 tccaggaagt gcggtcataa ccgaaagcgg cagaccggct gtgcaaaaag ttgcagaatt
      241 aatcagtaat tttgaaaaca aggtcgtgat tgaagggcat acagacaaca ttccccacca
      301 tgatcagaaa tttgatagta attgggagtt gtctgccggc agagcgattg ccgttctcag
      361 acatttagtt gaacagcgaa atattgaccc tagccgttta tcagcagtcg gctatggtga
      421 gtacaatccg cttgtcccta atgatacggc cgaacagcgt gctgcaaatc gaagagtcaa
      481 tattgtgttg gtctacgaac cggagggagc agaataatgg aaaaagaagc acagttgccc
      541 aaaaagcaga gcattccgaa gcttgttctg atagctgtca ttttgatggt tttaatggtc
      601 ggcggaggaa tgatcggttc tttgatgacg aacgggctag caacggaatg gatcgctaac
      661 ttcaacaata aagatgcgga aagcatcctt gttccgcaag aggaatttct ggtgaattta
      721 aaatccaatg atggcagttt ggatgatttt ttgaaaattg aaatgtccgt agagacaact
      781 gatgaggaca atggagccat tctgaccgaa aaggcggcaa ttgtgcgcga tgcgatcatt
      841 agtgtgctca gaaataaatc tacagccaac attttcgatg aatcggaggg tgtcctggtc
      901 atcaagcagg aaatcatttc gaaaatcaat caaagtttgg gctcggaaat cgcgagtgat
      961 ttatacatta caaatatcgt catgcaatag cgaagtggaa ggtgtgcgtc aaggaaatgg
     1021 aaaatgaact gttatacatc gggaaaagtt ttatcgtgtt gatttttgtg atttttgcga
     1081 taaattatct gttgaaatta ttggatcgat tcgtcaacaa gaacggcaaa tacatcacca
     1141 tgatagagcg tgttccattg aaccgcacat cgtcgctttg tatcgttaaa gtctcggaat
     1201 cctactattt gatgagctgt acagatacaa agaatgagat tttgaaggaa ttgaacattg
     1261 aagacatcaa ggaagaaaag actgttttgg acnnnnnnnn nnaaaagatt ccgcccggcc
     1321 agcccagtgg ttttggatga agtcgatgag ggtggcagaa tttcacgaag agggatgaga
     1381 aaataggatg gtgaaaaaag ggattgtcac aagcggcgtt ggattgttat ttctgacatt
     1441 tcctcaaaaa gttcatgcgg cggaaatcgt agacacggtt caaaatgctc tgggcgggac
     1501 atcggatgct gtaaaaatgt atgtgttgat aggtcttctg agtgtcatac cggcattttt
     1561 gatattgact acatgtttta cgcggatcat cattgtcttt tcttttttga gaagttcgtt
     1621 aggcacacag caaaatcctc ccaatactgt cttgataggc ttggcggcct tcttgacatt
     1681 ttttatcatg aggccagcct atacggaagt ttaccatgag gccgcggttc cgttgatgga
     1741 ggatcagatc ggcatcgaag agttctttta tatagcagag gatccgatcc gggactttat
     1801 gctgaagcag acgagggaga aggatcttac cctctttctg gatctgcagg gggatgacat
     1861 gccaaccaat acgggagatg taagtttgct cagtctcatt ccggcctttg cgatcagcga
     1921 attgcggacg gccttcacga tcgggttttt gatcttcatt ccctttctgg tgatagacat
     1981 cgtggttgca agtattttga tgtccatggg gatgttcatg ttgtcgccgg taatgatttc
     2041 gttgccgttt aagttacttc tgttcgtctt ggtggatggg tggtatttgg tcgttgagtc
     2101 gctggtatta ggttttcaat aggggtgcat gaatgtcgat agaaaaagtt ttagatgttt
     2161 tgagagaagg cttcatgacc attctgatag ttgccggacc gacgctggcc atcgccttgg
     2221 tggtaggctt gatcatcagt attattcaag cagcaacaca gctgcaggaa cagacattga
     2281 gttttgttcc aaaaatattg gccgtaatcg gagccttggt tgtgttcggc aattttatgc
     2341 tgaacgcatt gatcgcttat acagaaaaaa tattcgagtt gatcgctgga ttgtgatggc
     2401 taaaggatgc tgattacatt acagaaactc atattgatct ttatgcggat aggttctttc
     2461 gtttttataa gtaagggatt ttcattcaaa ggtgtaccaa ctgttgtgaa gattgccatt
     2521 tctttgggct tgagtttacc ggtttacatc atgatacc
//
LOCUS       DAOMUJ010000264         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11662_length_2549_cov_1.801123, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000264 DAOMUJ010000000
VERSION     DAOMUJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11662_length_2549_cov_1.801123"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..996
                     /gene="mmuM"
                     /locus_tag="PLT73_06795"
     CDS             76..996
                     /gene="mmuM"
                     /locus_tag="PLT73_06795"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HRM20122.1"
                     /translation="MNFKEWQKNQKVILIDGSMSLGLEEQGLDLNDELWTAKALVNEP
                     DKIEKVHQNYYDAGANIAITASYQATVAGFERLGHTTEESRALIKRTVELAKQAQTKS
                     QGLQEKWVAASVGPYGAYLADGSEYRGNYGLSKTELVDFHRERLELLLESGADLLAIE
                     TIPDLTEIQAVIELLAQHPKTTAWLTVTLKDDHHLCDGTDLRVFQLLAESSEQIIAYG
                     VNCVQPDLVLPALEYLKEIATKPLVAYPNSGATYDATTKVWTHSHAVDEVFSNEALKW
                     HESGCKWIGGCCCTSAKEIRLLKETFAAIL"
     gene            complement(1258..1497)
                     /locus_tag="PLT73_06800"
     CDS             complement(1258..1497)
                     /locus_tag="PLT73_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRM20123.1"
                     /translation="MKILYPATFEKDGGYILVQFPDIPEAMTQGATAQEAHAKAKEVL
                     GLALDGKQDFPEATAVDVLERKYPDKTVTLIEIDF"
     gene            complement(1725..2114)
                     /locus_tag="PLT73_06805"
     CDS             complement(1725..2114)
                     /locus_tag="PLT73_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRM20124.1"
                     /translation="MVLYYAIFNPEGTQFNISFPDLPELFTSGDDMSDALHMAKDALE
                     GYLLWLEDEKEAFPPASEYDEISPSKGDLLIPIEANLEVARLKENTKMIKKTLTIPNY
                     VNALAEKEGINFSQTLTEVLKEKLQIH"
     gene            complement(2161..2343)
                     /locus_tag="PLT73_06810"
     CDS             complement(2161..2343)
                     /locus_tag="PLT73_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRM20125.1"
                     /translation="MDSRELISIIMNDGWVLVNIRGIHHQFRHLLKPGRVTVPHPKKD
                     LPKGTVHSILKQAGLK"
BASE COUNT          725 a          524 c          575 g          725 t
ORIGIN      
        1 tcttttatta ccagttattc ttcagcaaaa aggcttctgt agcaatcgaa aatcaagaag
       61 tgggtgagct ttccgatgaa ttttaaagag tggcaaaaga atcaaaaagt gatcctcatc
      121 gatggttcga tgtccttagg tctggaggag caaggcttgg acctgaatga tgagttatgg
      181 accgcgaaag cattagtgaa cgaaccggat aaaattgaaa aagtccatca gaactattac
      241 gatgccgggg caaacatcgc catcacagcg agctatcaag cgacggttgc cgggttcgag
      301 cgtttgggcc atacaaccga agaaagccgg gctctgatca aaagaacggt agaactagcg
      361 aaacaggccc aaacgaaaag ccaaggcctg caagaaaaat gggtggcggc ttcggtcgga
      421 ccatacggag cctatttggc ggacggctct gaatatcggg gcaattacgg tctgtcgaaa
      481 accgaattgg tcgatttcca tcgcgaaagg ttagagttgc tgctggaatc cggggctgat
      541 ctgctggcca tcgaaaccat tccggatcta acggaaatcc aggctgtgat tgagttgcta
      601 gcccaacatc ccaagacgac agcttggctg acggtgactt tgaaggacga tcaccattta
      661 tgcgatggga ccgatctgcg tgtattccag ctattggcag agtcgtccga gcaaatcatt
      721 gcctacggcg tcaattgcgt gcagccggat ttagttttgc cggccttgga atatctgaag
      781 gagattgcga caaagccatt agttgcttat ccaaactcgg gagcgaccta cgatgccact
      841 acaaaagtat ggacgcacag tcatgcggtt gatgaagtat tctctaacga agctttgaag
      901 tggcacgagt caggatgcaa atggatcggc ggctgttgct gcacatcagc caaagaaatc
      961 agattgttga aggaaacctt tgctgcaatt ctttagttgg aataaacata ataaaataaa
     1021 cccatcatcc tttatgaaaa ataagaggat gatgggtttt gtgtctttta gtagtggggt
     1081 ggtcataccc cgagtatttc tttcaagcct tgtttatgac tttcgtactg ctttcattta
     1141 tggtttgttg tatacaaatc aagtaaagtg gtgagtactg tgaggaaaat caaaacaaag
     1201 ctttgaaatg gaacggcagc tgttgctacg cgtcctcaaa aaaactcaag ccgactgtca
     1261 gaaatcgatc tcaatcagag tgactgtttt atccggatac tttctctcca agacatccac
     1321 cgcagttgct tcaggaaaat cttgcttacc atctaatgcc aagcccaata cttctttcgc
     1381 cttggcgtgt gcttcttggg cagttgcacc ttgtgtcatt gcttcaggta tatcggggaa
     1441 ctggacgagt atatagccac cgtctttttc gaatgttgct ggatataaaa ttttcatatt
     1501 tcgctccttc caggacatcg gactaactga aattttataa caaaaaaatc ctcgataatc
     1561 cacagaataa attcggttcg ccaaccaata ttcaggactt aggaggattt ttgtcttgcg
     1621 acatgaatag tgtatcagat ttttatttgg gttgtaaata tcaaatggta ttaaagagag
     1681 acctcatctc caacgaggat tgctatatac gaacatccaa ctgcttaatg tatctgtaat
     1741 ttctccttca acacttccgt caatgtttgc gaaaaattga tgccttcttt ttccgcgagt
     1801 gcattgacgt aattcggaat cgtcaaagtt ttcttgatca ttttcgtgtt ttccttgagt
     1861 ctagccactt cgagatttgc ttcgatcgga atcaacaaat ctcccttaga aggagaaatt
     1921 tcatcatatt cggatgccgg aggaaaagcc tctttttcat cctcaagcca aagcagatag
     1981 ccttccaaag catcttttgc catgtgcagg gcatcggaca tgtcatctcc gctggtgaac
     2041 aactccggta aatccggaaa cgatatattg aattgcgtgc cttcgggatt aaaaatagca
     2101 taatacagta ccataaggat acttcctttc tattgttgaa taaatttaga aggcgagggg
     2161 ttatttcaac cccgcttgtt tgaggatgct gtgcactgta cctttaggta aatccttttt
     2221 tgggtgcgga actgtaacgc gcccaggttt tagtaggtgt ctaaattggt ggtgaatgcc
     2281 tcttatgtta actaataccc aaccgtcatt catgattatc gaaattaatt cccttgagtc
     2341 catggtaagc ctctttcttc atacgtaata tagtacgtat ttttgttgtt gtcaaataga
     2401 acctatccta aacatacgaa gtttacttca aatagataca ggaagtgtag cggcccatta
     2461 agtattagtg gctgcatata tagtctgaat aaaataaccc ccctacagaa atgctggatg
     2521 gaagcatttc tgtagggggg ttattaaaa
//
LOCUS       DAOMUJ010000265         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11689_length_2543_cov_2.793006, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000265 DAOMUJ010000000
VERSION     DAOMUJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11689_length_2543_cov_2.793006"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLT73_06815"
     CDS             <1..836
                     /locus_tag="PLT73_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM20126.1"
                     /translation="TEENDFYGSRIPIGGIAGDQQAALFGQTGFEQGMVKNTYGTGAF
                     IVMNTGLTPVKSENGLLTTIAYGLNGEINYALEGSIFVAGSALQWLRDEAELIRSAAE
                     SEEYAELLESNDNVYMVPAFVGLGAPYWDQDVRGAFFGLTRGTSKAHLIRATLESLAY
                     QTKDVVDTMQKDSGLVITNMRVDGGAAHNNFLMQFQSDLLNTTLTRSKVLETTALGAA
                     YLAGLAVGFWKDTDDIIQNWQPDCEFHPQMATEKREDLYAGWQNAVAAARHFKHKPKR
                     A"
     gene            1080..1970
                     /gene="rapZ"
                     /locus_tag="PLT73_06820"
     CDS             1080..1970
                     /gene="rapZ"
                     /locus_tag="PLT73_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRM20127.1"
                     /translation="MADTLELVVITGMSGAGKTVALQTFEDLGYFCIDNMPPSLLPKF
                     WELVKESGKISRICLVIDLRSRAFFDEIMSAVDSLDNTTFITTKVIFLDSKDDVLVSR
                     YKETRRSHPLTQSGGTVLEGIRKERELLEDIRNRSQLVIDTSETTPRQLRERLLDTFK
                     VDDNDVFHIEVVSFGFKYGLPIDADIVMDVRFLPNPHYVDELRPLTGLDKPVYDYVMK
                     QPETEIFYKKFLDLLEYIIPGYKKEGKTSVNIAIGCTGGQHRSVALAERTSLQLESAG
                     YQVNTTHRDRNKRKETVNRS"
     gene            1967..>2543
                     /locus_tag="PLT73_06825"
     CDS             1967..>2543
                     /locus_tag="PLT73_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRM20128.1"
                     /translation="MSQNPSRTKKIVVIGGGTGLPVILEELKNHNTDITAIVTVADDG
                     GSSGIIRDHANIVPPGDIRNCMIALSDMPKLEKDIFQYRFHSKDSFFSGHAIGNLIIV
                     ALTEMKGSIFDAIRLLSVMMGVKGKIYAAADEPLSLYAEFKDGTVVKGESKIAKGRKK
                     IERVYVKPLDETREAVASRHVINSILEADMVV"
BASE COUNT          714 a          565 c          657 g          607 t
ORIGIN      
        1 atacggagga aaacgatttt tacggatcgc gtatcccgat tggcggaata gccggcgatc
       61 agcaggctgc tctctttgga cagaccggtt ttgaacaggg aatggtgaaa aatacttacg
      121 gtacgggggc gtttatcgtc atgaacaccg gccttacacc ggtgaaatcg gaaaatggct
      181 tgcttacgac gatcgcttat gggttgaacg gcgaaataaa ctatgctctt gaaggcagca
      241 tttttgtggc cggttctgct ttgcaatggt tacgcgatga agctgagctg atccgatcag
      301 cggcggaatc ggaagagtac gccgaactgc ttgaatcgaa cgataatgtc tacatggttc
      361 ctgcttttgt tggattaggt gccccctact gggaccaaga tgtgcgcggc gctttcttcg
      421 gattgaccag agggacttca aaggcgcatc tgatccgtgc gacactcgaa tcgctggctt
      481 atcagacgaa ggatgttgtg gacacgatgc agaaggattc cggacttgtc atcacgaata
      541 tgcgtgttga tggaggggca gcgcacaaca attttctgat gcaattccaa agcgatctgc
      601 tgaatacaac cttgaccaga tccaaagtat tggagacaac ggcactggga gccgcatact
      661 tggcaggttt ggcagtagga ttctggaagg acaccgatga cattatccaa aattggcagc
      721 ctgactgcga gttccaccca caaatggcga ctgaaaaacg cgaagacctc tatgctggct
      781 ggcaaaacgc agtagccgcc gcccgccact tcaaacacaa acccaagaga gcgtgatgat
      841 tcgcggttag ggggtgagca ctaagcgggc tagccgtaat gggtttgttt tcgcccatga
      901 aggttagcca gattaggcgg aatcccctgc gaatcagaac gcgtttaaga aagagcgtga
      961 tgatttgcgt tcagataacc gaacaaaact taagaatcac taaaatattc agaaacagac
     1021 aaatgataca cccggtatgt tataatgggt gtatctattt tttgactgga ggaattaata
     1081 tggcagacac tctcgaattg gtagtcataa cggggatgag cggtgccgga aaaacggtag
     1141 cgttgcagac atttgaagat ttaggctatt tctgtataga caatatgccc ccaagtttgt
     1201 taccgaaatt ttgggaactt gtaaaagaat cgggtaaaat aagcagaatc tgcttagtga
     1261 tcgatctgcg ctcccgggca ttctttgatg aaatcatgtc tgctgtggac agcttggaca
     1321 acactacttt cataacgact aaggtcatct tcctcgattc gaaagatgac gtgcttgttt
     1381 cccgatacaa agaaacaaga agaagtcatc ccctgaccca atcaggcgga accgtactgg
     1441 aaggaatccg taaagagaga gaattgcttg aggacatccg caatcgctca cagttggtca
     1501 tcgatacaag cgagacaaca ccccgtcaat taagggaacg ccttctcgac actttcaaag
     1561 tggacgataa tgacgttttc catatagaag tcgtatcttt tggtttcaag tatggtttgc
     1621 cgattgatgc cgatatcgta atggatgtgc gctttttgcc caacccgcat tacgtagatg
     1681 aattacgtcc actgacgggg cttgataagc cagtctatga ttatgtcatg aagcaaccgg
     1741 agacggaaat attttacaag aaatttcttg atttattgga atacatcatt cctggttata
     1801 agaaagaagg caagaccagt gtgaatatcg ccattggttg tacaggcggg caacaccgct
     1861 cggttgcttt agcagaaaga accagccttc aattagaaag cgcggggtat caagtgaata
     1921 caacccaccg cgatcgtaat aaacgcaaag agacggtgaa tcggtcatga gtcaaaatcc
     1981 gagcagaacg aagaaaatcg tcgtaatcgg cggcggaacg ggcctgccgg tcattctgga
     2041 agaactgaag aatcacaata cagacatcac ggctattgtc acagtcgcgg atgacggcgg
     2101 cagcagcggc atcatccgcg accatgccaa cattgtgcct cccggggata ttcgcaactg
     2161 tatgatcgcg ctttccgata tgccaaaact ggaaaaggat attttccagt accgtttcca
     2221 ttcgaaggat tccttctttt ccgggcacgc catcggcaac ctcatcatcg tggccctgac
     2281 tgaaatgaaa gggagcattt tcgatgccat tcggttgctg tccgttatga tgggcgttaa
     2341 aggcaaaatc tatgccgcag ctgacgagcc gctctcactc tatgcagagt tcaaggacgg
     2401 cactgtcgtc aagggtgaat cgaaaatcgc aaaaggccgt aaaaagatcg agcgcgtgta
     2461 tgtcaaacct ttggatgaaa caagggaggc tgtcgcttcc cgtcacgtca tcaacagtat
     2521 cctggaggcg gatatggtcg tta
//
LOCUS       DAOMUJ010000266         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11855_length_2518_cov_1.651644, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000266 DAOMUJ010000000
VERSION     DAOMUJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11855_length_2518_cov_1.651644"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2408
                     /locus_tag="PLT73_06830"
     CDS             <1..2408
                     /locus_tag="PLT73_06830"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20129.1"
                     /translation="RWSYLHTGELNFCESSRDVPPLQGIIGQDRAVKSMDFGLGMAVP
                     GYNIFVSGPPGTGKSTYVQTVVSRLAEKGNTPDDWCYIYNFTDRDKPIAVSLPAGQGK
                     IFRNDMTEFVEDMRSLIPKTFESSDYDQQKGTIIQVVQEKVDSIFRSIEQEALKAGFI
                     VRQAPGRVALIPIRDGKQLSPEEYKALSAEERKIIDENTYKLEKRLDEIIRGSRALEK
                     EADKQLKELDTQITRFATEPPIARLKGKYAYSEKILDYLDKVLTDITENNIIFRLADA
                     PQAQNPFQLPESDGDPFIKYKVNLFVNNENSKGAPAIIEPFTNYYNIFGKIEYKNQFM
                     YTTTDFTMVKAGAIHQANGGYLVLQAKDVLFDPFMWDALKKVLKHKQALIENIGEQYR
                     YVPTLTLKPETIPLNVKIILIGSPIFYKVLTYDEDFRKLFKVKVDFDISMERNEENIR
                     KYVSFISSICEETGTLHFDRSGLGKVIEYGSRLAGNQTKLSTQFNEITEIVHESSAIA
                     KYEEATIVSDSHVRKALADRKYRANMIEEKFQEMLLKDKIMIDIKDEVIGQVNGLSVI
                     QTEEYAFGHPTRITARTYIGSNGVTNIERETKMSGSSHSKGVLTLAGFLGGQFAQEKP
                     LAVSAQLTFEQNYGGVDGDSASSAELYALLSSLADVPLKQNLAVTGSINQKGEIQPIG
                     GATEKIESFFDLCEAKGLNGGQGVIIPNQNVDDLMLKEEIIAAVEAAKFHIYSVKTVA
                     EGIELLTGMNCGKREPDGKYTQGSVFHKVQQKLEKYNQSIEAAQIASSQHMDDNASEY
                     EED"
BASE COUNT          774 a          582 c          619 g          543 t
ORIGIN      
        1 tgcgttggag ttatctgcat accggtgaac tgaatttttg cgaatcatcc agggatgtcc
       61 ctcccttgca gggcatcatc ggtcaggatc gggccgtgaa atcgatggac ttcgggttgg
      121 gaatggcagt gcccggctac aatatctttg tttccggacc accgggaaca ggaaagagca
      181 cctacgtcca gaccgtggtg tcccgtttgg ccgagaaagg caatacgccc gatgattggt
      241 gctatatcta caattttacc gatcgggaca aaccgattgc ggtgtctctg cctgcagggc
      301 aaggcaaaat attccgcaat gacatgaccg agttcgtaga ggatatgcgc agcctaattc
      361 cgaaaacgtt cgagagcagt gattatgatc aacagaaagg caccatcatc caagtggtcc
      421 aggaaaaggt tgattctatt tttcggagta tcgaacagga agctctcaaa gcgggattca
      481 tcgtcaggca agctcctgga cgcgtggcct tgatcccgat cagggacggc aagcagcttt
      541 caccagaaga atacaaagct ctttcggcag aagaacgcaa aatcattgat gaaaatacct
      601 acaagcttga aaagaggctg gatgagatca tccgcggttc ccgcgcacta gaaaaagagg
      661 ctgataagca gctaaaggaa ttggacacgc aaataacccg atttgcgaca gagcctccca
      721 tcgccagatt aaaaggaaag tacgcttatt cagaaaaaat cttggattat ctcgataaag
      781 ttttgacgga catcaccgaa aacaacatca tcttccggtt ggccgatgca ccgcaagctc
      841 aaaatccttt ccagctgccg gaaagcgacg gtgacccgtt cataaaatac aaagtcaatc
      901 tctttgtgaa caatgaaaac agcaaaggcg ccccagcgat catcgaacct tttacgaact
      961 attacaatat cttcgggaaa atagaataca aaaaccaatt catgtacacg actacggact
     1021 tcacgatggt gaaagcaggc gccatccatc aggccaacgg cggttatctg gtgcttcagg
     1081 caaaagatgt gctgttcgat ccgttcatgt gggatgccct gaaaaaggtt ctgaagcata
     1141 agcaagcatt gatcgagaat atcggcgaac aataccgcta cgtgccgacc ttgacgctga
     1201 aaccggaaac gattcccttg aacgtcaaaa tcattttgat cgggagcccg atattctaca
     1261 aagtactgac ctatgatgaa gatttcagga aactgttcaa agtaaaagtc gatttcgata
     1321 tcagcatgga acgcaacgaa gagaatatca ggaaatatgt ctctttcatc agctcaatct
     1381 gcgaagaaac tggaaccctc catttcgacc gcagcggctt agggaaagta attgaatatg
     1441 gatcacggct tgcgggcaac cagaccaagc tgtccaccca attcaatgaa atcactgaaa
     1501 ttgtccatga atccagcgcc atcgccaagt acgaagaggc aacgatcgtt tccgatagtc
     1561 atgtgagaaa agcgctggca gaccggaaat acagggccaa tatgatcgaa gaaaaattcc
     1621 aggaaatgtt attgaaggac aagatcatga tcgatattaa ggatgaggtc atcggtcaag
     1681 taaatggcct ctcagtgatc cagacagaag aatatgcttt tggccacccg acgcggataa
     1741 cagcccgtac ctatatcggt tcgaatggag tgacaaacat tgagcgcgaa accaaaatga
     1801 gcggcagcag ccattcaaaa ggagtactga cgttggcagg ctttctcggc gggcagtttg
     1861 cacaggaaaa accgctggca gtaagcgccc aattgacttt tgaacagaat tacggaggtg
     1921 tcgacgggga cagtgcctcc agtgccgaac tttatgcgct cttatccagc cttgctgatg
     1981 ttccgctgaa gcagaatctg gctgtcacgg gatccatcaa ccagaagggt gaaatccaac
     2041 cgatcggcgg cgccaccgaa aaaatcgaaa gctttttcga tctatgcgag gctaaaggct
     2101 tgaacggagg acagggggtc atcattccga atcagaacgt ggacgatctc atgctgaagg
     2161 aggaaatcat cgcggctgtg gaagctgcca aattccacat atattccgtc aagaccgttg
     2221 cggaagggat tgagctattg accggaatga attgcggaaa acgcgaaccg gatggaaaat
     2281 acacacaagg cagcgtcttc cataaagtcc agcagaagct ggaaaaatac aaccaaagca
     2341 tcgaagctgc tcaaattgca agcagccagc atatggatga taacgccagt gaatatgaag
     2401 aagactgatg ctgatccagc attttgattg gcacccaggt cgtatttcat agaaatcgaa
     2461 aagcagagac aggccattta gccttctctg cttttttgtg acactaagtc cgtaaaac
//
LOCUS       DAOMUJ010000267         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11861_length_2517_cov_2.393582, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000267 DAOMUJ010000000
VERSION     DAOMUJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_2517_cov_2.393582"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLT73_06835"
     CDS             complement(<1..434)
                     /locus_tag="PLT73_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRM20130.1"
                     /translation="MKNSGIYVSLDIGTTSIKVVVAEYVKDQMNIIGVGNEISKGLSR
                     GVIVDIDETVESIRSAVSQAERKANIQISNVIVGIPSNQISIEPCHGMYAVASENKEI
                     TDKDVQNVFAAAKVRSVPPEREIISVIPEDFIVDGFDGIRDP"
     gene            complement(690..1634)
                     /locus_tag="PLT73_06840"
     CDS             complement(690..1634)
                     /locus_tag="PLT73_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRM20131.1"
                     /translation="MAFKKIRQKLKSKGSSEGTPWQRATAELEKQNKAEKKQKTSKKS
                     GMKHLSREKKAKLKKSKHLPEATGTDKRQRYFGWYGIFSVGLVLSGFLISPYGKVKDI
                     YVEGAQAVPEQSIIDASHINGNLTALGVVWNDDKVDAFITDVLPQVKTAEVTLRGLND
                     ITIHVTEHETIAYVSSDGYYYNVLDNGTVANTELKVPIGNNPIITSFEMNQNLDDLLE
                     QYMQLTDSVQNSISEIKYTGTEENPYTITVYMNDGNEVKASLPSFAEKILYYPDIVSQ
                     LGETKGTIDLEVGVYFTPFTQTTEEDASASADSAILSE"
     gene            complement(1653..>2517)
                     /locus_tag="PLT73_06845"
     CDS             complement(1653..>2517)
                     /locus_tag="PLT73_06845"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRM20132.1"
                     /translation="SISDSKKIIKAFQPDVVIGTGGYVCAPVLYSASKLGIPTIIHEQ
                     NSVAGVTNKFLSRVVDRICICFEDARSDFSAYPDKIVFTGNPRAQEVASLKECADLKE
                     YGLKEGVPTVLIFGGSRGAKKLNESFVAAYPLFKDKPYQVLLATGDVHFNAVEQEISA
                     LTDRLDNVRIVPYIHDMPKLFKRTDLIVSRSGATTLTEVMALGLPSILIPSPYVTNNH
                     QQKNAESLVKNGAAEMILEKDLSAQSLFEAIDKLMIDETVRKEMALQAKQMGITDASD
                     RIIDVILALKN"
BASE COUNT          589 a          611 c          536 g          781 t
ORIGIN      
        1 ctcggatctc tgataccatc gaatccatct acgatgaaat cttcagggat gacggaaatg
       61 atttctctct cgggcggaac agaacggact tttgcagcag cgaagacgtt ctggacatcc
      121 ttatctgtga tttccttgtt ttcgcttgcg actgcataca ttccgtggca aggttcgata
      181 ctgatttggt tgcttggtat gcctacgatg acgtttgata tctgaatgtt cgcttttctt
      241 tcagcttgac tgactgcact gcggatcgat tcgaccgttt catcgatatc aacgatgact
      301 ccacggctaa gacctttgga aatttcattc ccgactccaa taatgttcat ttgatctttc
      361 acatattctg cgactacaac ttttattgaa gtggttccaa tatctagact gacgtatatt
      421 cctgaattct tcatggtttt attgaaacct ccgtttgaca taatggcttg tgtaaacatc
      481 cgcattgttc cgaaaaaaac tatcggttac ttatacgcga tgacagaaag ctataccttt
      541 tcattttatc ataataactc ttcatttacg aagattttta caaataattc tatccgatag
      601 aagtttggca aaagttattc aatccctcaa caatgatcgc aatcattgtt caaggattga
      661 ctgcaagtca atcctgatct cttttccact cattcggata gaatggcgga atctgccgaa
      721 gcagatgcat cctcttctgt tgtttgcgtg aagggtgtaa agtagacgcc cacttctaga
      781 tcgatggtcc ctttcgtctc tcccaattgg cttacgatgt caggatagta caagattttc
      841 tcagcgaagg atggcaagct cgccttcact tcattcccat cgttcatata taccgtgatt
      901 gtgtatggat tttcttccgt tccggtatat ttgatttccg agatgctgtt ctggacactg
      961 tccgtcaact gcatatattg ttcgagcagg tcatccagat tctggttcat ttcaaagctc
     1021 gttatgattg ggttgttgcc gatcggaacc ttcagttccg tattggctac ggtcccgtta
     1081 tccaacacat tgtagtaata gccgtccgat gaaacataag caatggtttc gtgttccgtt
     1141 acatgaattg tgatgtcatt caggccacgc agcgtcacct cagctgtttt gacttggggc
     1201 aaaacatcag tgataaaggc atcgacctta tcatcattcc agacgactcc caaggcggtc
     1261 agattcccat tgatgtgact ggcatcgatg atgctttgtt ccggaacagc ttgggcgcct
     1321 tccacataga tgtccttgac tttcccgtag ggggagatca gaaaaccgct gaggaccaac
     1381 ccaaccgaga aaatgccgta ccatccaaag tatctttgac gtttgtctgt tccagtggct
     1441 tcaggaaggt gtttactttt ttttaatttc gctttttttt ctctcgacaa atgcttcatg
     1501 ccggattttt ttgaagtctt ttgctttttc tcagctttgt tttgcttttc caactcagca
     1561 gttgcacgct gccagggagt cccttccgac gaacccttac tcttcagctt ctgccggatt
     1621 tttttgaaag ccatctggcc tccctccctt tctcaattct tcaaagccag gatgacatcg
     1681 atgatgcggt ctgatgcatc tgttataccc atctgtttgg cttgaagagc catttctttg
     1741 cgtaccgttt catcgatcat cagcttatcg atagcttcaa acaatgattg tgccgaaagg
     1801 tctttctcga ggatcatttc tgcagccccg tttttcacta ggctttcggc atttttttgc
     1861 tgatggttgt ttgtgacata agggctcgga atcagaatgc tcggcaaacc caaagccatc
     1921 acttccgtca acgtggttgc gccgctgcgc gatacaatca agtcggtacg cttgaataat
     1981 ttcggcatat catggatgta cggcacaatc cggacattgt ccaaacgatc cgtcagtgcg
     2041 ctgatttctt gctccactgc attgaagtgg acgtctccgg tagccagcag cacttgatat
     2101 ggtttgtctt tgaagagcgg ataggccgct acgaaagatt cattcagttt cttggctccc
     2161 cggctacctc caaaaatcaa aacggtcgga acgccttcct tcaatccgta ttccttcagg
     2221 tcggcacact ccttcaagga ggctacttcc tgcgctcggg gattccccgt gaacacaatt
     2281 ttatccggat aggcggagaa gtcactacgg gcatcctcaa aacaaatgca gatgcggtcc
     2341 acaacccggc tcaaaaattt atttgtgact cctgcgactg aattttgctc atggatgatg
     2401 gtcggtatgc ccagtttgga ggccgaatag aggacaggcg cgcaaacata accacccgtc
     2461 ccgatgacga catccggttg gaaggccttg atgattttct tcgagtccga aatgctc
//
LOCUS       DAOMUJ010000268         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11884_length_2512_cov_3.361416, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000268 DAOMUJ010000000
VERSION     DAOMUJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11884_length_2512_cov_3.361416"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLT73_06850"
     CDS             <1..675
                     /locus_tag="PLT73_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20133.1"
                     /translation="GQTLGILGETGSGKTTLVNLVGRFYDPTSGTVLIDGKSAKDYPV
                     RQLRENIAMVMQDVFLFSDTIEDNIAFGNPQVDRSYIQQMARIADADGFITSMPQGYD
                     TIVGERGVGLSGGQKQRISLARALAKDPAILILDDTTSAVDMETESKIQQELLGLTEN
                     KTTFIIAHRISSVRDADLILMMEKGQVVEQGTHAELLAKRGKYYDVYCKQLGLTGGEI
                     NGTQYV"
     gene            656..>1923
                     /locus_tag="PLT73_06855"
     CDS             656..>1923
                     /locus_tag="PLT73_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20134.1"
                     /translation="MARNTYDVDESLEQEFNWGHYRRLAGYIRPYKKPISKILAVIIV
                     ANIAGMAGPYFTKIAIDDLIPSGNVPQLLGLGGIFILSLVLIGWCMRYRIYAITEVGQ
                     DILKDMRYDIFAHLQRLPFAYFDNRPHGKILIRVVNYINTLSDLLSNGLINLISDLFN
                     VVITLGFMLLIDVRLTLYSMALLPVLFGLVLFIKKRQRIAFQILSNKQSNLNAYIHES
                     ISGIKITQSFAREQVNYDIFNEVSEEYRTSWMKAVKIQFLMWPGVLNISVLTTCLIYF
                     VGIRQIGVSVSTGTLIAFIGYINNFWNPVINIGNFYNSLITATAYLERIFETLDEVPS
                     IQDAPDAYELPPVKGAVDFENVTFRYEEGKDILHKVNFHIEPGQSIALVGPTGAGKTT
                     VINLLSRFYDINEGSVKIDGHDVRDVTLGS"
     assembly_gap    1924..1933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1934..2444
                     /locus_tag="PLT73_06860"
     CDS             <1934..2444
                     /locus_tag="PLT73_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20135.1"
                     /translation="SQMGVMLQDTFIFSGTILENIRYGKLDATEEEVIAAAKVVRAHD
                     FIQNLKDGYHTVVEERGSTLSAGQRQLLSFARALLADPKILILDEATASIDTRTEELL
                     QEGLQKLLKDRTSFIIAHRLSTIKNSSRIFYVAGGNIAEAGTHEELMAKKGLYHHLYQ
                     SQYDLLQAV"
BASE COUNT          645 a          667 c          619 g          571 t
ORIGIN      
        1 ggacaaacgt tggggatttt gggcgaaacc ggctcaggca agacgacgct cgtcaatctg
       61 gtaggaagat tctacgatcc cacttcggga accgtcctga tcgatggcaa aagtgccaaa
      121 gactacccgg tccgccaatt gcgggagaac atcgccatgg tcatgcagga cgttttcctg
      181 ttttccgata cgatcgagga caacatcgct ttcggcaatc cgcaggtgga ccgttcctac
      241 atccaacaaa tggcccggat cgccgatgcg gatggcttca tcacaagtat gccccaagga
      301 tacgacacga ttgtcggcga acgcggagtc ggtttgtctg ggggtcaaaa gcagcgtatt
      361 tctttggcac gggcattggc gaaggatccg gccatcctta ttttggatga caccacttcc
      421 gcggtcgaca tggaaaccga atcgaagatc caacaggagt tgcttggatt gacggaaaac
      481 aaaacgacct tcatcatcgc ccaccggatt tcgtcggtgc gggatgcaga tctgatcctg
      541 atgatggaaa aaggccaagt cgtcgaacaa ggaacccatg cggaactttt ggccaagcga
      601 ggcaaatact atgacgttta ctgcaaacaa ctcggattga ctggagggga aatcaatggc
      661 acgcaatacg tatgatgtcg acgaaagctt agaacaagaa ttcaattggg gacattaccg
      721 gcgcttggcc ggctatatcc gcccctacaa aaaaccaatt tcaaaaatat tggctgtcat
      781 cattgtggcg aacatcgccg gcatggcggg accttatttc acaaaaattg caatcgatga
      841 cctgataccg tcaggaaatg tgccccagct gttgggtttg ggtgggatct tcatcctttc
      901 ccttgtgctg atcggctggt gcatgcgcta tcggatctat gccatcaccg aagtcggcca
      961 ggacattctg aaagacatgc gctacgatat ttttgcacat ctgcagcgcc tgccgtttgc
     1021 ttacttcgac aaccggcccc acggcaagat tttgatccgc gtagtcaatt acatcaatac
     1081 gctgagcgac ctgctgagca atgggctcat caatctgatt tctgaccttt tcaatgtagt
     1141 gatcacattg ggcttcatgc ttctcatcga tgtgcgtttg acgctttaca gcatggctct
     1201 tttgccggtt ctcttcggac tggtgctgtt catcaaaaag cgccagcgga tagcctttca
     1261 gattttgagc aacaaacagt ccaacctcaa cgcctatatc cacgaaagca tttcgggaat
     1321 caaaatcacc caatcgtttg cccgcgaaca ggtcaattat gacatcttca atgaagtcag
     1381 cgaggaatac cggacgtctt ggatgaaagc cgtcaaaatc caattcctga tgtggcccgg
     1441 tgtactgaat atttcagtac tgacgacctg cctgatctat tttgtgggca tccgtcagat
     1501 cggtgtgtcc gtttcgaccg gaacattgat cgccttcatc ggctacatca acaatttctg
     1561 gaatcccgtc atcaatatcg ggaacttcta taattccttg atcactgcca cggcctattt
     1621 ggagcgcatc ttcgaaacat tggacgaggt gccttccatc caggatgcgc cggacgccta
     1681 cgagttgccg ccagtcaaag gcgcagtcga cttcgaaaat gtaaccttcc gttatgagga
     1741 aggcaaagat attttgcaca aggtcaactt ccatatcgaa ccgggacaga gcatcgcttt
     1801 agtcggtcca acaggtgcgg gcaaaacgac cgtcatcaat ctgctgagcc ggttctacga
     1861 catcaatgaa ggcagcgtca aaatcgatgg tcacgacgtc cgcgatgtca ccctcggctc
     1921 cttnnnnnnn nnncagccaa atgggcgtca tgctgcagga taccttcatc ttctccggta
     1981 cgattctgga aaacatccgc tacggcaagc tcgatgcaac cgaggaggaa gtgatcgcag
     2041 ccgcgaaagt tgtacgcgcc catgatttca ttcaaaacct gaaggacggc taccacaccg
     2101 ttgttgaaga gcgcggcagt accctatcgg ctggccaacg acaattgctc tcctttgcca
     2161 gggccctgct tgccgatccg aagatcctga ttctcgacga agctaccgcc agcatcgata
     2221 ccaggaccga agaactgctg caggaaggcc tccagaaact gctgaaagac cggacttcct
     2281 tcatcatcgc ccaccggctg tcgacaatca aaaacagctc ccggatcttc tacgtggcag
     2341 gaggcaatat cgccgaagcc ggcacccatg aagagctgat ggccaaaaaa ggcctgtatc
     2401 accatctcta ccaatcccaa tacgacctgc tgcaggccgt gtgaagaagg aaaagcggaa
     2461 cgggttcgtt cagccctgaa agaaatttag gaaatctggc cctgaatgag ca
//
LOCUS       DAOMUJ010000269         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11892_length_2511_cov_2.119707, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000269 DAOMUJ010000000
VERSION     DAOMUJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_2511_cov_2.119707"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLT73_06865"
     CDS             <1..293
                     /locus_tag="PLT73_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014608448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRM20136.1"
                     /translation="EVQQLRLLTRRRKAYTERITQCKNEIHNILQRANIKLTSYLSDI
                     YGLTGMALLEMFINGEVITEETDLPKIHGRIKATVDQLVEAMDGKLSIASTS"
     assembly_gap    319..328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1069..1317
                     /locus_tag="PLT73_06870"
     CDS             1069..1317
                     /locus_tag="PLT73_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20137.1"
                     /translation="MAVYILSPLLVPYFAVAMASHIAIDTLNHKRVKLFYPLPGGIRL
                     GLCSANGIVNDWLFKIGSLVVVIEMVLLIGRMGLAFFS"
     gene            complement(1558..1887)
                     /locus_tag="PLT73_06875"
     CDS             complement(1558..1887)
                     /locus_tag="PLT73_06875"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HRM20138.1"
                     /translation="MGENFELIMTLVSIGIISVFGYLIINMVLNLTAVEVTEKATLIG
                     KDTENASHMNGDSMVTTTTYVLTFEVDGGERITFRVGRGVYRKYVIGDYGRLTYKRKW
                     FRQFERI"
     gene            2289..>2511
                     /locus_tag="PLT73_06880"
     CDS             2289..>2511
                     /locus_tag="PLT73_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HRM20139.1"
                     /translation="MRKNTEFNSKDLKWIREPKNYSITENKIEIVTEPHTDLWQRTYY
                     NFRNDNAPVLQINTDEKYFSFIVKTEFESN"
BASE COUNT          727 a          545 c          511 g          718 t
ORIGIN      
        1 aagaagttca acaattgcgg cttctgacaa gacgtcggaa agcctatacc gaacgcatca
       61 cccaatgcaa aaatgaaatt cataatattt tacaacgggc taacattaag ctaaccagtt
      121 atctttcaga tatctatggt cttactggaa tggcattact cgagatgttc attaacgggg
      181 aagtcattac agaagaaacg gatcttccca aaattcacgg gagaatcaaa gctactgtgg
      241 atcagctagt tgaagcgatg gacggtaaac tgtccatcgc ttcaactagc tgatccacag
      301 tagctttgat tctcccgtnn nnnnnnnntt gaggatcgat ttctattagg acaatccctg
      361 gaacattacc gtcatttggt aaatcaagta gatgaaatca cggttgtaat caagcgatat
      421 gttttagaga gatttgagcg aaattggccc aaatgtagag gccttccaat cacaaggcaa
      481 cttagcatcg tgggcgggag tatgcccagg atcttaagag agtgctggaa taaaaaagtc
      541 ttcccatacg acacaaggaa ataaatattt aaagtgcatc cttttccagg ccggaggcac
      601 tgcggggaga tcaaaagatg aagcattcca taatctatac gctcgtatct ctggtaggag
      661 ttcaaagatg aaggcaatca tcgcctgtgc acataaaatg attcgagtga tctataaaat
      721 cttgagcact ggcgagcatt acgacacgaa aaaagctcta ggactgcggc aacaggctag
      781 agcaatttaa aaaaattttt tccacttaaa ttatagtatg ggagtgactt tttgcgcatt
      841 tttcagctat ttattttcaa acaaaagagg ttcaaaaacg tttatataca acctgtattc
      901 ccggatagtc gtaactaggt cggataaaac cttgatataa aagcatttct ccctagttat
      961 gcgtttaaca cataactagg tgaatcgaac ctcaaaatac tgtccaaacc ttgatttgac
     1021 gggttttgca tttgttccta gttaggtgtc tccgggaatc cagggagcat ggctgtctat
     1081 attctgtctc cgctgttggt cccttatttt gcggttgcga tggcatccca tattgcgatc
     1141 gacacgctga accacaaacg cgtgaagcta ttctatccgc tgcccggcgg catccgcctc
     1201 ggcttgtgca gcgcgaacgg catcgtgaac gactggttgt tcaaaatcgg cagtctggta
     1261 gtcgtcatcg aaatggtcct gctgatcggc agaatgggat tggcattctt ttcatagctc
     1321 taaactaaaa aacacaagta ttccggcaac agtttagccg ggatgcttgt gtttttgttc
     1381 atgaccggcc gcagagttgc tccgtactcc cgtgagatta gtcttggccg gaggtggaat
     1441 tccggatacc cccttcctcc ggttaagcgg tctttgcagg cgttaagacg ctttaacttt
     1501 gctgttcttc ggcgaaatct gctttcgtcg gaactggaat ccctctatgc agtcctctca
     1561 aatccgttcg aattgtctga accattttct tttgtacgtc agccgcccat aatcaccgat
     1621 gacatacttg cggtataccc ctctgccaac acggaaagtg atgcgttcgc cgccgtccac
     1681 ttcgaatgtc aacacgtagg ttgttgtcgt aaccatagaa tcaccgttca tgtggctcgc
     1741 gttctccgta tctttcccaa tcaatgtagc tttttcggta acctcaacgg cagtcagatt
     1801 caaaaccata tttatgatca gatatccaaa tacactgatg attccgatac tcacaagagt
     1861 cattatcaac tcaaaattct cacccattcg acatcacact ccttgttaac aggatagcac
     1921 tttttagcgg aatattaaaa tcaatcaccg tctaatataa gagtcgggtt tgccgaatat
     1981 cggactaaca ttcgacaagt tcattccagt tttgggcgaa attgtcgaat actgcctagc
     2041 attcgacaat ctgactcctt tgcagaacga gtttgtcgaa tacataacgt ctattcggct
     2101 attttgatcc taaatccata ccgccctcgt attggttcgc ggcggttgca ccgccccata
     2161 gacatgtttc cggaaatgct ttattcactg tcattatcct ctttgtcttt gtgtctgaac
     2221 acttatgtct ccttccatta ttgattaagg ttataatatc agcataaaca gttacggagg
     2281 tttaatagat gaggaaaaat acggaattta atagtaaaga tttaaaatgg ataagggaac
     2341 ctaaaaacta tagtattacc gaaaacaaaa tagaaattgt aacagagcct catactgact
     2401 tatggcaaag aacctattac aattttagga atgataatgc gcctgttttg caaattaata
     2461 cggatgaaaa atacttttcc tttatagtaa aaactgaatt tgaaagcaat c
//
LOCUS       DAOMUJ010000270         2497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_11980_length_2497_cov_1.885749, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000270 DAOMUJ010000000
VERSION     DAOMUJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11980_length_2497_cov_1.885749"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PLT73_06885"
     CDS             complement(<1..1419)
                     /locus_tag="PLT73_06885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM20140.1"
                     /translation="MKPNIKNNEVGVFALGGLGEIGKNMYGIQFQDEIIILDSGIMFP
                     EDDLLGIDYVIPDYSYLVQNQTKIKGLFISHGHEDHIGGVPFLLKELNVPIYAGKLAL
                     AMIRNKLDEHGLLRDAVLHEINEDSVIKFRKTSISFFGTTHSIPDTMGIVVKTPPGNI
                     VFTGDFKFDFTPANGKPANIHKMAKIGEEGVLLLLSDSTNAETPTFTQSEQIVGQSLK
                     NIIQKVSGRIIFASFSSNIYRLQQVTEVALETGRKIAIFGRSMETNFRTARELGFIVA
                     PDDLFIETRELNSLPADKVLIMCTGSQGEPMAALSRIANGTHRQISIQPGDTVIFSSS
                     PIPGNTTSVNRVINLLLEAGAAVVHGKVNNVHTSGHGGQQEQKLMLTLMKPKYFMPVH
                     GEYRMLKIHTELAESVGIPAENCFISDNGEILALTADSARRAGNFTAADVYVDGKGIG
                     DIGNIVLRDRRVLSEDGLVVVVL"
     gene            complement(1416..1634)
                     /locus_tag="PLT73_06890"
     CDS             complement(1416..1634)
                     /locus_tag="PLT73_06890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HRM20141.1"
                     /translation="MIFKVTYQPAKFDAPTRENTESLYVEASDIVIARRLVEQNTPYN
                     IEFIQPLEGNHLEYEQKNPDFKITEFNV"
     gene            2001..>2497
                     /locus_tag="PLT73_06895"
     CDS             2001..>2497
                     /locus_tag="PLT73_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM20142.1"
                     /translation="MSLIWRYISRYNKLFLVNVLGAFGFVFVELGLPTMLARLINETV
                     AGGGKENILRTTVFMVGLVLIGFVGRVIVAYATGRITTNMVADMRNDMYQNIQTFSHE
                     EYDRIGVSSLVTRVTNDAFILMQFAQMLLRMGLMTFIMMFASAFMIARTSPELSVILL
                     PAFPAL"
BASE COUNT          645 a          501 c          561 g          790 t
ORIGIN      
        1 caacactacg accaccagac catcttcaga aaggacacgc cgatcgcgca acacgatgtt
       61 tccgatgtcg ccgatgcctt ttccgtcaac gtatacatcg gcagcagtga agttgcccgc
      121 tcgtcttgcg gaatcagctg tcaaagcaag gatttcgcca ttatcggata tgaaacagtt
      181 ctcagccgga atgccgactg attctgctaa ttctgtatga atcttcaaca tacgatactc
      241 accatgaacc ggcatgaaat atttaggttt catcaaggtc agcatcagtt tttgttcttg
      301 ttggccacca tgtccggagg tgtgcacatt gtttactttt ccgtgaacaa cggccgctcc
      361 agcttccaaa agcagattga tgacgcggtt gacgcttgtt gtgttgcctg gaatcggtga
      421 acttgagaaa atgacagtat cgccgggttg gatggaaatc tgtctatgtg taccattggc
      481 gattcggctc aaagcagcca tcggttcacc ttgagagccc gtacacataa tcagcacttt
      541 atccgctgga agactgttca gttcgcgcgt ttcgatgaaa agatcatccg gagccacgat
      601 gaagcccaac tctctggctg tgcggaagtt cgtctccatg ctgcgtccga atattgcgat
      661 tttccgaccg gtttcgagcg cgacttcagt cacttgttgc agtctgtaga tattggacga
      721 gaaggaagca aaaatgattc ttcctgaaac tttttgaatg atgtttttaa gggattgacc
      781 tacgatttgc tctgattgag taaatgtagg tgtttctgca tttgtgctgt cgctcagaag
      841 caaaagcaca ccttcttctc cgatttttgc catcttatgg atattcgccg gtttgccgtt
      901 cgcaggtgtg aaatcgaatt tgaaatcacc tgtgaataca atgttgcctg gaggagtctt
      961 gaccacgatc cccatggtat ccggtatgct gtgggttgtt ccaaagaagc tgatgctggt
     1021 tttacggaat ttgatgacag aatcttcatt gatttcgtgc aggaccgcat ccctgagtaa
     1081 accgtgctca tccagtttat tgcggatcat ggcgagtgcc aatttgcctg cgtagattgg
     1141 gacattcaat tctttcaaaa gaaagggaac accaccgatg tgatcttcgt gtccatgaga
     1201 aatgaacaag cctttgattt tggtttggtt ttgcaccaaa taactgtagt cgggtatcac
     1261 ataatcaatg ccaagcagat catcttcggg aaacatgatc ccggaatcaa gaatgatgat
     1321 ttcatcctgg aattgtatgc catacatatt tttgccgatt tcccctaaac ccccgagggc
     1381 aaatacgccg acttcattat tttttatatt aggtttcata cattaaactc cgtgatttta
     1441 aaatcaggat ttttttgctc atattctaaa tggttgcctt ccaaaggctg gatgaattca
     1501 atgttgtagg gggtattttg ttcgaccaat cttctggcta ttacgatgtc ggatgcctcc
     1561 acgtaaagag attccgtgtt ttctctggta ggtgcatcaa attttgctgg ttggtatgtt
     1621 actttaaata tcatatagtt cactccattt ttctttttgt ttttgatagt actgtactta
     1681 aaaattacaa tcttagtttg ttaacttggt caatagttgt attttttcat acaatcggta
     1741 ctgtaagttt accacaatta aggcatttag tatagaaatg actagcaaaa tcatttttgc
     1801 agaagtgcaa gcctttccat tagtaaggca attctctttc gtttttttta tcaaaaggaa
     1861 tcaatatttt tgtagactaa aatagcgaaa ttggctagac tagataggaa ggatttgaca
     1921 agactagaga aaaaagaata gaagcttttt ttgcttgaaa aaatctgaag aatcgtttct
     1981 attaaggtag gtgtatcgaa atgtctttga tttggcgtta tatcagtcgg tataacaaat
     2041 tatttttagt gaatgtattg ggagcttttg gattcgtgtt tgtggagctg ggcttaccca
     2101 caatgcttgc acgtttgatc aatgaaacgg tggctggcgg aggcaaggag aacatccttc
     2161 gcacgactgt ttttatggtc ggcttagttt tgatcggttt tgttggaagg gtcatcgtcg
     2221 cctatgcaac cggaagaatc acaacaaata tggtagcaga tatgcggaat gacatgtacc
     2281 aaaacatcca aacattttct catgaagaat atgatcgcat cggcgtgtcg tcattggtga
     2341 cgcgggttac caatgacgct ttcattctga tgcaatttgc ccaaatgctg ttgcgtatgg
     2401 gtttgatgac tttcattatg atgttcgcga gtgcttttat gatagcgcgc acgagtccgg
     2461 aactatcggt tatcctattg ccggcttttc cggcact
//
LOCUS       DAOMUJ010000271         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12214_length_2457_cov_2.552040, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000271 DAOMUJ010000000
VERSION     DAOMUJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12214_length_2457_cov_2.552040"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLT73_06900"
     CDS             complement(<1..184)
                     /locus_tag="PLT73_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM20143.1"
                     /translation="MEFLTDRGYTKNQKVVNQLKPVATYEEIEQQRGDKSILYVDVRS
                     PSEYAKSTIPGAVNVPV"
     gene            complement(285..1733)
                     /locus_tag="PLT73_06905"
     CDS             complement(285..1733)
                     /locus_tag="PLT73_06905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM20144.1"
                     /translation="MEHTTFESFAISEEIITALKALRYFKPTQVQQEVIPLTLKGKDI
                     IVESQTGSGKTVSFGVPLCESVSWEENRPQALILVPTRELALQVKEDITNIGRLKRIK
                     ATAVFGKTSFDTQKSELRQKSHIVVGTPGRVLDHLQKGTMKFDKIRYLVLDEADEMLN
                     MGFIEQVEAIIDFLPKQRQTLLFSATMPSEVVRLASFYMKNDRVSVKMESDEASKPKI
                     LQSFIRVQESGKAKQLLDLLTVENPDSCIIFCNTQEAVNGLYTFLSKAGLPIDKMHGG
                     MVQDDRTAVMEDFRKGRLRYLVATDVAARGIDVDNVTHVVNYDVPEEKESFIHRTGRT
                     GRAGKTGVALTLVTPSEERSWQGVKDFAQQEITEIDPPSARFVQHHKAAFEKKLQERP
                     VIKRARNRELNKDITKVYFNGGKKKKLRAIDFVGTLTNIPGIDADDIGIITIQENVTY
                     IEILNGKGPHVISEMQNRTVKGKALKVHKAKK"
     gene            complement(1714..2217)
                     /locus_tag="PLT73_06910"
     CDS             complement(1714..2217)
                     /locus_tag="PLT73_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20145.1"
                     /translation="MGNWQDIHEDLKIFKLISGDGGLGSVYKAEYDMFGKMVPVNIEV
                     QQSELFPEEFIMRAAIRVDTFDPKEDSFVRQNYTAKAEEAGTTLNLESIQNIPYIDKH
                     KTFDKEEYLEKRDEVAQQAIERIRLFCEDQQRKRDYNKSFVPFAFFCYTEAIKKVKVG
                     QDGTYNI"
BASE COUNT          528 a          648 c          528 g          753 t
ORIGIN      
        1 ggaccggtac atttacggcg cctggtatag tcgacttcgc atattcggaa gggctcctga
       61 catccacata caaaatcgat ttgtctccgc gttgctgttc tatttcttca tacgttgcga
      121 ctggtttcaa ttggttcacc actttctgat tcttagtata gcctctgtcg gtcaggaatt
      181 ccacccaaaa tcttgaggca agcgcatgaa aacgacccga caagctcctt atttaacgga
      241 gacatgcggg tcgtttcgca ttcaatcgtt caattttgct gcatctattt ctttgcttta
      301 tgcactttca acgctttgcc tttgacggtg cggttctgca tctcagaaat cacatgcggc
      361 ccttttccgt tcaggatttc gatgtaggtc acgttttcct ggattgtgat gatgccgatg
      421 tcatccgcat cgataccggg aatgttggtc agcgtaccta cgaaatcaat ggcgcgcagt
      481 tttttcttct tgccgccgtt gaaatagact ttggtgatgt ccttgttcaa ttcccggttg
      541 cgcgcgcgtt tgatgacagg ccgttcctgc agtttctttt cgaaagccgc tttatgatgc
      601 tgcacgaagc gggcgctcgg cggatcaatt tcggtgattt cctgctgcgc gaagtctttc
      661 actccctgcc agcttctttc ctcactaggc gtaaccaagg tcaaggctac ccccgttttg
      721 ccggcgcggc cggttctgcc ggtacggtgg atgaaacttt ctttctcctc gggaacatcg
      781 taattgacga catgggtcac gttatcgaca tcaatcccgc gcgcagccac atccgtagcc
      841 accaaataac gcagcctgcc tttgcggaaa tcttccataa cggcagtgcg gtcatcctgc
      901 accatcccgc catgcatctt atcaatcggc aaaccggctt tacttaagaa tgtgtacagt
      961 ccgtttacgg cttcctgcgt attgcaaaag atgatgcaac tgtccggatt ctcaacggtc
     1021 agcagatcca atagttgctt ggctttgccc gattcttgga cacggatgaa ggattgcaga
     1081 atcttcggct tggatgcctc atcgctttcc attttcaccg atacgcggtc gtttttcatg
     1141 tagaagcttg ccaaacgaac cacttcactc ggcatggtcg cggagaatag cagcgtttgt
     1201 ctttgtttcg gaaggaaatc gatgatggct tccacttgct caatgaagcc catattcagc
     1261 atctcatccg cctcatcaag taccaggtag cgtatcttgt caaatttcat tgtccctttt
     1321 tgcagatgat ccaaaaccct gcccggggtg ccgactacga tatggctttt ctgtctcaat
     1381 tccgattttt gcgtatcgaa ggaagttttg ccgaagacag cagtagcctt aatccgcttc
     1441 aaccgtccga tgttggtgat gtcctctttc acttgcagag ccagctccct tgtcggaacc
     1501 aaaatcagcg cttgcggacg gttttcctcc cagctcacac tttcgcacaa cggtacgccg
     1561 aaactgacgg tcttgccgct accggtctgc gattcgacaa tgatatcttt gcccttcaag
     1621 gtcagcggga tgacctcctg ttgtacttgc gtcggtttga aataacgcaa tgcctttagt
     1681 gccgtaatga tttcttcgct gattgcgaag ctttcaaatg ttgtatgttc catcttgtcc
     1741 tactttcact ttttttatcg cttcagtata acagaaaaat gcaaaaggaa cgaaactttt
     1801 gttgtagtcc cttttccgtt gctggtcctc gcagaacagc ctgatccgtt caatggcttg
     1861 ctgcgcaacc tcatcccgtt tttccagata ctcttcttta tcgaacgttt tgtgcttatc
     1921 aatgtaggga atattctgaa tactttccaa gtttaaagtc gttccggcct cctccgcttt
     1981 ggccgtgtaa ttttggcgga cgaaagaatc ttccttcgga tcgaacgtgt ccacccggat
     2041 ggctgcgcgc atgatgaatt cctcggggaa cagttccgat tgctgcactt cgatgttcac
     2101 cggcaccatt ttcccgaaca tatcgtattc tgctttgtag acactgccca gcccgccgtc
     2161 cccgctgatc agcttgaaga ttttcaggtc ctcatgaatg tcttgccaat tccccacatt
     2221 ggcggcgtat tgccatactt ttcacagcgg tgcttcgata aaaatgcttt ttttgatgtg
     2281 gcttttgatg tagtccattt tattcatcct tcctactgat tgatcctatc cacacttaat
     2341 aagcaactat gataacgtta tctttagctt cattataatc aaatgaattc ccttagacaa
     2401 acttggctcg ctaaacttcg aaaaccactc ttggcaagaa caccattctt tgttaaa
//
LOCUS       DAOMUJ010000272         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12215_length_2457_cov_2.131141, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000272 DAOMUJ010000000
VERSION     DAOMUJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12215_length_2457_cov_2.131141"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLT73_06915"
     CDS             complement(<1..423)
                     /locus_tag="PLT73_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRM20146.1"
                     /translation="MTELSLNTIKKQLQEAGFKLTPQREATVSVLLEKEKEHLSAEEI
                     FLLLRLKHPDIGLATVYRTLEILTELNITYKVVFEDGLSRYDLRRTSNEHFHHHLLCI
                     ECGNIEEIHEDLLGEVEKDVEARYQFVVKDHRLTFHGIC"
     gene            complement(457..1323)
                     /locus_tag="PLT73_06920"
     CDS             complement(457..1323)
                     /locus_tag="PLT73_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRM20147.1"
                     /translation="MSKVLGTIITGVIVDENEKSVFVQKDGITYRLDKDESETALQLG
                     DTVKGFVYEGMNKQLRMTLKEPASQVGKYGWGTVVAVRKDLGVFVDIGLDDKEIVVSL
                     DNLPDIKALWPKKGDRLLIALEQDSKDRLWGIIADEPVFRSISRTPGEELKNKDVTGT
                     VFRLKLVGTFILTDDHYIGFIHPSEREIEPRLGEVVSGRVVGISQHGMLNLSLKPRAH
                     EAISEDAAMILVLLEQSPTHSLPYWDKSDPEAIKEYFGISKAQFKRALGHLLKARLII
                     QEDGHTKLIKKD"
     gene            complement(1545..>1859)
                     /locus_tag="PLT73_06925"
     CDS             complement(1545..>1859)
                     /locus_tag="PLT73_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRM20148.1"
                     /translation="IGDKSVIGKAVVALSAEEAIAKVTEDCILVLKNSDKDYTPAFEK
                     AAAVIVEAGGLTSHAAVVGIANGIPVIVGAKNVTSLVQDNELITIDSRRGIIYRGATT
                     TI"
     assembly_gap    1860..1869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1870..2457)
                     /locus_tag="PLT73_06930"
                     /pseudo
     CDS             complement(1870..2457)
                     /locus_tag="PLT73_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411157.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
BASE COUNT          586 a          611 c          513 g          737 t
ORIGIN      
        1 gcagatgcca tgaaaagtca aacgatgatc cttaaccacg aattgatagc gcgcttcgac
       61 atccttttcg acctcaccca acaaatcttc gtgtatttcc tcgatattgc cgcattctat
      121 gcacaaaagg tgatgatgga aatgttcatt gctggtcctt cttaaatcgt agcgggataa
      181 cccgtcttca aacacaactt tgtaggtgat gttcagctcc gtcaagattt ccaaagtccg
      241 atagaccgtg gcaaggccaa tgtcgggatg cttaagccgc aacagcagga aaatttcctc
      301 cgcactgaga tgctcttttt ccttttccag cagcacgctg acagtcgctt ctcgttgcgg
      361 agtgagcttg aatcctgcct cttgcaattg ttttttgatg gtatttaatg acaattccgt
      421 cactgctgtc ggctccttta aggacaggct gaagacctaa tcctttttga ttaatttagt
      481 gtgtccgtct tcttggatga tcagtctggc tttcaacaaa tggcccaaag cccttttgaa
      541 ctgcgctttg ctgataccaa agtattcttt gattgcttct ggatcactct tgtcccaata
      601 aggcaagcta tgcgttgggc tctgctccaa caacaccaga atcatggctg catcctcact
      661 gattgcttcg tgagcacgcg gtttcaagga cagattcagc atgccgtgtt gactgatgcc
      721 gacaacccga ccgctgacga cttcgcccaa gcgcggttca atctcccgct cggatgggtg
      781 aatgaatccg atgtaatgat catccgtcag gatgaacgtt cctactaatt tcaaacggaa
      841 gacggttccg gtgacgtcct tatttttcaa ttcctcaccc ggagtacggg aaatggaacg
      901 gaataccggt tcatcagcaa tgattcccca aagacggtct ttgctgtctt gctctaaggc
      961 aatcagtaat cgatctcctt tttttggcca tagcgctttg atgtcgggca agttatccaa
     1021 agaaaccaca atttctttgt cgtcaaggcc gatatccaca aacacgccca aatctttccg
     1081 gactgccaca actgttcccc agccgtattt tcctacttgg ctagctggtt cttttaaggt
     1141 catgcgaagt tgcttgttca tgccctcgta tacgaaacct ttaacagtgt cccccaattg
     1201 caatgctgtt tccgattcat ctttatccaa tctgtaagtg atgccgtcct tctgaacgaa
     1261 gacactcttt tcgttctcgt ccacgatcac acccgtaatg attgtaccta atactttact
     1321 cataattctc ctccagacgg tccttccgtc gctgtatttc ttccatttta tcatatcaca
     1381 aaatgatttc caaagtgtcg ttatcccgcg cttgacatat ttgttatatt tgagcatatt
     1441 ttcctatttg ccgatggaat catcaggaag ataggattca aacaaagcaa atgggtccga
     1501 gactctcgtc ccagacccaa agatgctctc taatttagtt cagcttagat ggtagtagtt
     1561 gcaccgcggt agatgatgcc acgacgagaa tcgattgtga tcaattcgtt gtcttgcact
     1621 aatgaagtca cattttttgc tcctacgatt accggaattc catttgcgat accaacaaca
     1681 gcagcatgac ttgtcaagcc gccagcttca acgattaccg ctgcagcttt ttcaaatgca
     1741 ggtgtatagt ctttatcaga gttcttcaat accaaaatgc agtcctctgt cactttagcg
     1801 attgcttcct cagctgaaag agctacaaca gcttttccga tgacagattt gtcgccgatn
     1861 nnnnnnnnnt ggttccgatc aattggatct tcatcaagtt tgttgttccg cgttcgccta
     1921 ctggtacgcc ggcagtgatg atgatcaagt cgccttcttt agcgaatttt tcttctttgg
     1981 caacttttgc agccaattcc atcatttcat cagttgaatc aggtttttcg gttacgaatg
     2041 gatacacacc ccatgaaagt gccaatccac gtctttgacg ctccgagaaa gtgactgcaa
     2101 cgatgtgtga tttcggacgg tatttagaga tcatgcgcgc tgtatgtcca gattcagtag
     2161 cggcaacgat tgtttggatg ttaaggttgc gggctgtatg tccaaccgat tgaccgattg
     2221 cttcagccat atctgtgttg ctgtatgctt tcaaagcaaa tgcatcttgt ccaacgatcg
     2281 cttcttcagt acggatagcg attgtagcca ttgtcgtaac ggcttcaacc gggtaatccc
     2341 cagcagctgt ttctccggat agcataactg catcagtacc gtcaaagatt gcgttggcaa
     2401 cgtcccctgc ttcagcacgt gtaggacgtg ggttgcgttg catggaatcc agcattt
//
LOCUS       DAOMUJ010000273         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12348_length_2437_cov_2.124685, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000273 DAOMUJ010000000
VERSION     DAOMUJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12348_length_2437_cov_2.124685"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1120
                     /gene="ispE"
                     /locus_tag="PLT73_06935"
     CDS             260..1120
                     /gene="ispE"
                     /locus_tag="PLT73_06935"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRM20149.1"
                     /translation="MEWIERAPAKINLALDVLYKRDDYYHELEMVMASVDLADHLTFT
                     PLKEDEIRIYTNKVFLPVDTRNHVYQAVKLLKDRCGIKKGIKIEIDKKIPVAAGLGGG
                     STDAAAALRALNKIWDLQLSVEELIAIGNQVGSDVPYSIVGGTAFVSGRGEIVRKLRP
                     MPSCWVILAKPRLSVSTKTIFRLLTVEELQHKSTSRVVAQAIEEGDYERMCQNVGNAL
                     EQVTFNKHPELRKLKDRMEKYGLDGVTMSGSGPTIIGFSRNTSRIKRAYNSLRGFCEE
                     VYMVRLLNEE"
     gene            1288..2256
                     /locus_tag="PLT73_06940"
     CDS             1288..2256
                     /locus_tag="PLT73_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRM20150.1"
                     /translation="MMLNRLKVTGILSAAAFFLVGCGASESSETGISEDGIKVVTTFY
                     PMYDFAKNVVGDNGEVSVLLDAGQESHGYEPTPQDIAAIAEADVFIYNSDEMETWVPS
                     VLESIGSSDVIVVEAAENIALFEMVEDAAAEEEHDEEEGHEEGEDAHNVDPHVWLDPV
                     YAQEEVDAILSGVLEADEANKESYEANAAVFNEKLAKLDASYQTAFETAENRTFVVQH
                     AAFGYIARRYELTEVAVSDVSSDAEPNPAKLAELQRFMIDNQITTVYYSDSASSKTAQ
                     TLAEATGAALEVLSPLEGITDEDQEAGKDYLSVMEENLEALKKVIQ"
     gene            2275..>2437
                     /locus_tag="PLT73_06945"
     CDS             2275..>2437
                     /locus_tag="PLT73_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20151.1"
                     /translation="MDYIDVQHLSFYYDDEPVLQDITFQVHEGEFVILTGENGAAKST
                     LLKSILGLLN"
BASE COUNT          701 a          477 c          651 g          608 t
ORIGIN      
        1 aaaatgccag tacgcttaaa ttagttaaga acttattcca aataaatcaa acagacatcg
       61 aattgtctgt ttttttttat ctaaaaatca caagctatgg gcggaattgt gcgggaaaaa
      121 cggttccggg caattaatca tgcgatattg caattaagct tcagaaacta attcttgttt
      181 gttggcatat atttctttta aaaagagcac gcttaagcta gtattgtatt ataagaacag
      241 tggagaggag cgtggagcga tggaatggat tgaacgtgcg ccggcaaaaa taaatttggc
      301 gttagatgta ctgtataagc gggatgacta ttatcatgaa ctcgaaatgg tgatggcttc
      361 agtcgacctt gcggaccatc ttacttttac tcctctcaaa gaagatgaaa tcaggatcta
      421 cacaaataag gtgtttctgc cggtggatac gcgtaatcat gtctatcagg cggtaaagct
      481 gctcaaagac cgttgcggga taaagaaggg aatcaagatt gaaatcgaca aaaaaatccc
      541 tgttgcagcc ggattgggcg ggggcagcac agatgctgcc gcggctctgc gcgcgctgaa
      601 taaaatctgg gatctgcagc tttctgttga agagctcatt gctatcggca atcaagtagg
      661 ctcggatgtt ccctacagca tcgtgggcgg taccgctttt gtatccggcc ggggtgaaat
      721 cgtccggaaa ttgcggccga tgccatcctg ttgggtgatt ttggcgaagc cgcgcctaag
      781 tgtctcgacg aagacgattt tccgtctgtt gactgtcgag gaattgcagc acaaatcaac
      841 ttccagagtc gtagcgcaag ccatagaaga aggcgattat gaacggatgt gccagaatgt
      901 aggaaatgct ttggaacaag tgaccttcaa taaacatccg gaactgcgca aattgaagga
      961 ccggatggag aagtacggcc tggacggtgt gacgatgagc ggcagtggcc ctaccatcat
     1021 cggcttcagc aggaatacat cgcgcatcaa acgtgcctac aacagtctgc gcggattctg
     1081 cgaagaagta tacatggtcc gactgctcaa tgaagaataa tcagaaatgc agtgtaagac
     1141 cgctctgcct caaccgcgga actccttagg atgttcgcgg tttttgcttg tgaaacggaa
     1201 gcgaaatggt tgacgcgttg cgtgcaggtg tgataaagtg tctatcggac cttaaacagg
     1261 aatcattacg ttttggagga ataataaatg atgcttaata gattaaaagt gacaggtatt
     1321 ttatctgcgg cagcgttttt tttagttgga tgcggagcaa gtgaaagcag cgaaacaggc
     1381 atttctgagg acggtattaa ggtagtcaca accttttatc cgatgtatga ttttgcgaaa
     1441 aatgtagtag gagacaatgg cgaagtttcc gttctgctgg atgctggtca agaatcgcat
     1501 ggttatgagc cgacaccgca ggatattgcg gctattgctg aggccgatgt gttcatctat
     1561 aacagtgatg aaatggaaac gtgggtgccc agcgtcttgg aatcgattgg atcgagcgat
     1621 gtgatcgttg tggaagctgc agagaatatc gcgttatttg aaatggtaga ggatgcggct
     1681 gccgaagaag aacacgatga agaagaggga catgaagaag gcgaagatgc acacaatgtc
     1741 gatccgcatg tctggttgga tccggtgtac gcacaagagg aagtcgatgc cattttatcc
     1801 ggagtacttg aggctgatga agccaacaaa gagagttacg aagcaaatgc tgctgttttt
     1861 aacgaaaaat tggcgaaact ggatgcatct tatcagactg cttttgaaac tgccgaaaac
     1921 cgcacattcg tcgtccagca tgcagctttc ggctacattg cccgtcgcta cgaactgact
     1981 gaggtggcgg tgtcggatgt gtcatccgat gcggaaccga atcctgccaa actggctgaa
     2041 ctacagcggt ttatgatcga taatcaaatc actaccgttt attactcgga cagtgcctcc
     2101 tcaaaaacag cacaaacctt ggctgaagca acaggtgccg ctctggaagt gttgagtccg
     2161 cttgaaggga taaccgatga agaccaagaa gcgggtaaag attacttgag tgtgatggag
     2221 gaaaatttgg aagctttgaa aaaagtgatc cagtaacaga agagaggaat tcgaatggat
     2281 tacatcgatg tacagcatct ttcgttctat tacgatgatg aacctgtcct gcaggatata
     2341 accttccaag tgcacgaagg ggagttcgtc attcttacag gcgaaaacgg tgctgcgaaa
     2401 tctactttat tgaagagcat cctgggactg ctgaacc
//
LOCUS       DAOMUJ010000274         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12492_length_2415_cov_2.857627, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000274 DAOMUJ010000000
VERSION     DAOMUJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12492_length_2415_cov_2.857627"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..997
                     /locus_tag="PLT73_06950"
     CDS             326..997
                     /locus_tag="PLT73_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HRM20152.1"
                     /translation="MKTRVKEISEELKLVISGKTVDAVLPPLLFAVVNSRYQLTVASI
                     MAIALALLLAIIRLARKQPLRYAMGGLVGVMFASAIAYFSNNAANYYLPKIMSSAMFV
                     LAALVSLIMGKPMAALTSHLTRGWDLEWYWRKDVKPAYQEVTIIWLLFFLMRLALQVI
                     LFRRGDVAELAWTNTLLGLPFTLAGLILSYLYGIWRLRNLKGPSVEEHREGKQQPWEG
                     QTRGF"
     gene            1028..1729
                     /locus_tag="PLT73_06955"
     CDS             1028..1729
                     /locus_tag="PLT73_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRM20153.1"
                     /translation="MKKVKKVGAKCHYNNRILNKCLVLLLSLLFFGCGTSNDDTSSQT
                     DLQELTKIEVTEYEGKDLSSLTDFRENSIKGPQYINTDVYTLTIDGLVEQPNSLTYDE
                     VLDNQKYTKVVTLHCVEGWSVDILWEGILLADLFEAVDIQESADTVIFHSEDGYSTAL
                     PLQTIMDRQLMIAYKMNGVVLPPERGFPFQLVAEDKLGYKWIKWITRIELSDDANYKG
                     YWEQRGFDNEADVNP"
     gene            1949..>2415
                     /locus_tag="PLT73_06960"
     CDS             1949..>2415
                     /locus_tag="PLT73_06960"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRM20154.1"
                     /translation="MILVIDNYDSFTYNLVQYLRQLDETVVVKRNDKITIEEIAALNP
                     LMILISPGPKTPNEAGISLAVVRHFAGTIPILGICLGHQTIAELFGAEIVKAKEPVHG
                     KVHAIQHTDKGVFQGLKNPLNVTRYHSLIVANGSLPEALEVTARTEAGEIMGFL"
BASE COUNT          633 a          513 c          646 g          623 t
ORIGIN      
        1 cgttgcgagg ccagatttcc taaattgtca tgactcttgc gatttcaatc gcttaatcct
       61 gtcatcaggc catccaactg atttcgtagt gaggccctct gatgtcgcac tgtgacatca
      121 gagtacttga ccgattaggt cacagtgttt ccttaatcag ggctgaacga acccgttcag
      181 ctttccttat tgaattggat caggtgccaa gtttccggat aacaccggtt tctgataaat
      241 gctttccttg cttggaaata gtataattgg ggcacataga aaggctgatt ttcagattta
      301 cagaaaatgg ggaggggatg atgatttgaa aaccagagtc aaggaaattt cggaagaatt
      361 gaaattggtc atctcaggga agacggtcga tgctgtattg ccgcccttac tgtttgccgt
      421 ggtcaacagc cgttatcagt tgactgtcgc cagtattatg gctattgctt tggctttgct
      481 gttggccatc atccggttgg cacggaagca accgttgcgc tatgccatgg gtggtttggt
      541 cggggttatg tttgcctccg caatcgctta tttttccaat aatgccgcta attattacct
      601 gcccaaaata atgtccagcg ccatgttcgt gttggcggcg ttggtgagcc tcatcatggg
      661 gaaaccgatg gccgctttga cgagccatct gacgcgcggg tgggatctgg aatggtattg
      721 gcgtaaggac gtgaagccgg cctatcagga agtgacaatc atttggttac tgttttttct
      781 gatgcgattg gctttgcagg tcattctttt ccgacgcggt gatgttgccg agttggcttg
      841 gacaaatacg ctcctaggac ttccgttcac gcttgctgga ttgatcctta gctatcttta
      901 cggcatttgg cgtctccgca acctgaaagg gcccagcgtg gaagaacacc gggaaggcaa
      961 gcagcagccg tgggaagggc agacacgtgg tttttagtcg gaagggtttc ggaaagaagg
     1021 cattttgatg aaaaaagtga aaaaagtcgg agcaaagtgc cactataata atcgaattct
     1081 caataaatgc ctagttctgt tgcttagtct gttatttttt ggttgtggaa catcgaatga
     1141 tgacacttca agccaaactg atcttcaaga actgaccaaa atcgaggtga ccgaatacga
     1201 ggggaaagat ttatcttcct tgaccgattt tcgggaaaac tccatcaagg gcccgcaata
     1261 tataaacacc gatgtctaca cgctgacgat cgatggtttg gtcgagcagc caaattccct
     1321 gacctacgac gaagtgctgg ataatcagaa gtacacgaag gttgtgacct tgcattgcgt
     1381 ggaaggatgg agcgtggata tcctttggga agggatcctg cttgcggatc tattcgaagc
     1441 agtggacatt caagaaagcg ccgatacggt catttttcat tcagaggatg gctacagtac
     1501 agccttgccg ttgcagacga tcatggacag gcaactgatg atcgcctaca agatgaacgg
     1561 tgttgtcctg ccgccggaaa gaggctttcc gttccaactg gtggcggagg ataaactggg
     1621 atacaaatgg atcaaatgga tcacccgcat cgaactgtcc gatgacgcga actataaagg
     1681 gtattgggaa cagcgcggct ttgacaatga ggcggatgtc aacccgtaaa aagagtgacg
     1741 acagtgaggg caggttgtaa ccgcgtatgc tgccgatttc gggaaagaca gaatggctgg
     1801 ggcagttgtc ctggtcattt tttcgtcaga agtcagtttt cccaaaacta taggctggag
     1861 tcccggcatt tggtatgatg gacctatcat tttaggagat ttgacagaaa gaacgataga
     1921 aaaaataaca atgatgatga gggaccgtat gatacttgta atagataatt atgattcctt
     1981 cacctacaat ctcgtccaat atctgaggca attggatgag actgttgtgg tgaaacggaa
     2041 cgataaaata acaatcgaag agatagcggc actgaatccg ctgatgatcc tgatttctcc
     2101 gggtccgaag acaccaaatg aagcgggcat cagtctggct gtcgtcagac atttcgcagg
     2161 aacgatcccg attctgggta tctgcttggg tcaccaaacg attgcagagc tgttcggcgc
     2221 ggaaatcgtg aaggcgaaag agcctgtcca tggcaaagtg catgccatcc aacacacaga
     2281 caaaggcgtc tttcaaggct tgaaaaaccc gctgaatgtc acaagatacc attccctgat
     2341 cgtggcaaat gggagcttac cggaagcgct ggaagtcact gcccggacag aagctggcga
     2401 aatcatgggc tttct
//
LOCUS       DAOMUJ010000275         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12496_length_2414_cov_2.036032, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000275 DAOMUJ010000000
VERSION     DAOMUJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12496_length_2414_cov_2.036032"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLT73_06965"
     CDS             complement(<1..363)
                     /locus_tag="PLT73_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRM20155.1"
                     /translation="MRKKVRWQLLLVLGAFFFSMMFAPSKNAQELPDPTSEFYVYDPS
                     GTLSEETKTFIMSVNEQYELTEEKPQVVVAVVDSLEGETIEEYSVDLFEKWKIGQADA
                     DNGVLILLALEEREIRFEI"
     gene            complement(392..970)
                     /locus_tag="PLT73_06970"
     CDS             complement(392..970)
                     /locus_tag="PLT73_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM20156.1"
                     /translation="MKGWLKGLIGVFIAVLLLGVPLISSYNGLVTEESNVDVQWENVE
                     TKLQRRYDLIPNLVESVKGAMEQEQEVFGAIADARARIGSAQTTEEQVAASNEMESAL
                     SRLLVIVENYPELKSNEQVTALMDELAGTENRISVERDRYNESVQQYNNKVKRFPGSL
                     MAGLFGFDEKSYFEAVSGAETAPSVDLNTADE"
     gene            complement(1060..1953)
                     /locus_tag="PLT73_06975"
     CDS             complement(1060..1953)
                     /locus_tag="PLT73_06975"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRM20157.1"
                     /translation="MLKIGSHVSMGGKKMLLGSAEEAASYGSSVFMIYTGAPQNTRRK
                     SVDEMNIPEGMDYMAKHGLVELVVHAPYIINLANTTKPDHFEFAIGFLREEIVRAQAL
                     GAKQITLHPGSHVGAGSEIGIKQIIKGLNEVLEPSQTAQIALETMAGKGTEIGRTFEE
                     LAAIMDGVVHNDKLSVTLDTCHTNDAGYNVKEDFDGVLEEFDRIIGLERLKMIHVNDS
                     KNERGAHKDRHANIGFGTIGFETLNKIVHHPQLQDLPKILETPFVGEDKKNKKAPYRF
                     ELDMLKSQTFNPGLLEDILNQ"
     gene            complement(2110..>2414)
                     /gene="aspS"
                     /locus_tag="PLT73_06980"
     CDS             complement(2110..>2414)
                     /gene="aspS"
                     /locus_tag="PLT73_06980"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357083.1"
                     /note="catalyzes a two-step reaction, first charging an
                     aspartate molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; contains discriminating
                     and non-discriminating subtypes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM20158.1"
                     /translation="LRIHTREVQEQMFAALGFSKEEAEEEFGFLMDAFDYGFPPHGGI
                     ALGLDRLVMLLAGESNIREVIAFPKNGKAFDPMTNAPSEVSAAQLKELSLKIASLD"
BASE COUNT          594 a          660 c          451 g          709 t
ORIGIN      
        1 gatctcgaag cggatttccc tttcttccag agccaacaaa atcagtacac cattgtcagc
       61 atccgcttga ccgattttcc atttttcaaa cagatcgaca gaatactcct cgattgtctc
      121 gccttccagt gaatcaacca ctgccacgac aacttgaggt ttctcctcgg tcaattcgta
      181 ctgctcattc acactcatga tgaatgtttt tgtttcttcg gagagcgtac cgcttggatc
      241 atagacataa aattcgctag tcggatctgg taactcctgc gcgtttttgg atggtgcgaa
      301 catcatcgaa aagaagaatg ctcccaaaac caaaagtagt tgccatcgca ccttttttct
      361 catttgaaac aaagcatcct caacccctca attattcgtc agctgtattc agatctacac
      421 taggtgcggt ttcagcgccg ctgaccgctt caaaataaga tttttcatca aatccgaaaa
      481 gtccggccat gagcgaccca gggaagcgtt tgactttgtt gttgtattgt tgcacagatt
      541 cgttgtatcg atccctttcg acagaaattc tgttttctgt gcccgccaac tcatccatca
      601 aagccgtcac ctgctcattc gatttcaatt ccggatagtt ctcgacaatc accaacagac
      661 gcgaaagtgc agattccatt tcgttgctgg cggcaacctg ttcttcggtc gtttgggcac
      721 tgccgattct ggcacgggca tcagcgattg cgccgaacac ttcctgttcc tgttccatgg
      781 ctccttttac tgattccacc aagttgggga tcagatcata acgtctttgg agcttggttt
      841 ccacattctc ccattgcaca tcgacattgc tttcctcagt caccaaacca ttataagagc
      901 tgatcaatgg cactcccaac aacaacactg caataaaaac accgattaaa cctttcaacc
      961 aacctttcat cttcccatct ccttcattca tttattttgt gcttgtatat aattatccct
     1021 gattccagac aaaaccgcat ccatccaaag atgtatttct tactggttca atatgtcttc
     1081 caataagccc ggattgaatg tctgcgactt cagcatatcc aattcgaatc tgtaaggcgc
     1141 ctttttgttc tttttgtcct cgcccacgaa cggcgtttcc aatattttcg gcaaatcctg
     1201 cagttgcgga tggtgaacga ttttattcag agtttcgaag ccgatggtgc cgaacccgat
     1261 attcgcatgt ctgtctttgt gggcccctct ctcgtttttt gagtcgttga catgaatcat
     1321 tttcaaacgt tccaggccga tgatgcggtc gaattcttcc aacacaccat cgaagtcctc
     1381 tttgacgtta tagcccgcat cattcgtatg gcacgtatcc aacgtcaccg aaagtttgtc
     1441 gttatggact accccatcca tgatggcagc cagctcttca aacgtccggc cgatctctgt
     1501 gccttttccg gccatcgttt ccaatgcaat ctgcgctgtc tgactaggct ccagcacttc
     1561 attcaagccc ttgatgatct gtttgatgcc tatctcagaa cctgcgccga catgcgaacc
     1621 tggatgcaac gtgatttgtt ttgcaccaag cgcttgcgcc cgcacgattt cttcgcgcaa
     1681 aaatccgatc gcaaattcaa aatgatccgg tttcgttgta ttcgccaaat tgatgatgta
     1741 gggtgcatgg acgaccaact cgaccagacc atgcttcgcc atataatcca ttccttccgg
     1801 aatattcatc tcatcgacac tttttctccg ggtattctgc ggagcgcctg tgtaaatcat
     1861 aaataccgaa gaaccgtatg acgctgcctc ctctgctgaa cccaacagca tttttttgcc
     1921 gcccatagaa acatgtgaac ctatctttaa catatcttca cccttttatt gtgtattatc
     1981 ctaaaaagca attccgccta atccaaagca acagacgaaa aaaaacagcc ttatgatacg
     2041 attattgtat cataaggcct gttttcatta ccatcagctg ctctttttag ttcaattgct
     2101 aatccaaggt tagtccaaag aggcgatttt caggctcaat tctttcaact gagctgcgct
     2161 tacttcgctt ggggcgttcg tcatcgggtc gaaagctttc ccgtttttag ggaacgcgat
     2221 cacttcgcgg atgttcgatt caccagccaa caacatgacc aaacgatcca accctaaagc
     2281 gatgccccca tgaggtggga acccgtaatc gaatgcatcc atcaagaaac cgaactcctc
     2341 ttcagcctct tctttactga aaccaagtgc cgcaaacatc tgctcctgta cttctcttgt
     2401 gtggatacgc aacg
//
LOCUS       DAOMUJ010000276         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12508_length_2412_cov_2.218074, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000276 DAOMUJ010000000
VERSION     DAOMUJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12508_length_2412_cov_2.218074"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLT73_06985"
     CDS             complement(<1..199)
                     /locus_tag="PLT73_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM20159.1"
                     /translation="MAKVTAGRNLLGDFAPKFAELNDDVLFGQVWSREKEFAPRDRSI
                     VTVTALITGGNFEQLPFHLNKA"
     gene            519..1394
                     /locus_tag="PLT73_06990"
     CDS             519..1394
                     /locus_tag="PLT73_06990"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRM20160.1"
                     /translation="MDKKRIVIYTLLFIATLAIATQGFTNTGLDGLLVPLLFTFAYFT
                     IIFIIATIIKNNSIVDIGWGMGFVVGSWLTLLVTENPTVLSYAIVGFITVWGLRLSLR
                     LLKRNYGKPEDFRYAQWRKEWGDKVVITAFFRVFVVQGIINFIVGSASYVVIKYNEFS
                     FDSAHRYFVYAALFIALVGLFFEVVGDEQLRQHINKKTRTLLQTGLWSITRHPNYLGE
                     ILIWIGLYASGITLFFTDSINPYYYLLLIISPILMSTVLIKISTPLLEKNMEKYDGWE
                     EYTKRVPMLFPFTKK"
     gene            1544..1957
                     /locus_tag="PLT73_06995"
     CDS             1544..1957
                     /locus_tag="PLT73_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013728557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM20161.1"
                     /translation="MSYSIGEFSKMIGLPVSTLRYYENEGLLKPDRDENNLRVYTDQD
                     LKWVTFLLHLKGTGMSMAELKQYTIWRAEGDATMLERMELLEKRRRLLEKDIQALMHN
                     LEVIENKIDFYEERLAGNKDSMISQVEAEKEKSTK"
     gene            2046..>2412
                     /locus_tag="PLT73_07000"
     CDS             2046..>2412
                     /locus_tag="PLT73_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3737 family protein"
                     /protein_id="HRM20162.1"
                     /translation="MMEHLNQQLFTGERALFKGKDLQISHSVFADGESPLKESNNIVL
                     ENTIFRWKYPLWYARNIQANNITLVETARSGIWYTNGITITDSMIQAPKIFRRAADIK
                     LERVEMPNAQETLWNCNDIT"
BASE COUNT          698 a          454 c          560 g          700 t
ORIGIN      
        1 tcgctttgtt caagtggaac ggaagctgct cgaagtttcc gcctgtgatg agtgcggtga
       61 cggtgacgat ggaacgatcg cgcggcgcga attccttttc ccgcgaccag acttgcccga
      121 acagaacatc gtcattcaat tcagcaaatt ttggtgcaaa atcacccaat aaatttcttc
      181 ctgccgttac ttttgccata aaatcatctc cttaggaatg ttagtttcag catactcctt
      241 agagtgcact ttaagtcaag cgcgagctca ggattctttt cttttctcaa aatattctgc
      301 tgttgtatct ttggaaatgg aggtgaaagc acatggcctg ttcaatttga gcattctctg
      361 aaaagggtag aaaattcggg cttcatttaa gaccttcgat tcgattgctt gtcatatatt
      421 agtgtataat gagattatta tagcttagtg atggaacgag ctgaattttt ttgttttttt
      481 agatggtagg atgcataaaa atttgaaaga ggtggcctat ggataagaaa agaattgtca
      541 tttatacgct attatttata gcaacattag cgatagcgac acaaggattc acgaatactg
      601 gtttggacgg actgcttgtc ccgttacttt ttacctttgc ctattttacg atcatcttta
      661 ttatcgcaac aatcattaaa aacaattcca ttgtcgatat tggttggggg atgggatttg
      721 ttgtagggag ctggctgaca ctgcttgtga ctgaaaatcc caccgtttta tcgtatgcca
      781 ttgttggatt tattacggtg tggggactta gattatcttt gcgcttattg aaaagaaatt
      841 acgggaagcc tgaagatttc aggtacgcac agtggcggaa agagtggggc gataaagtag
      901 tcatcaccgc ctttttcaga gtgtttgtgg ttcagggtat catcaacttt atcgttggat
      961 ctgccagcta tgtggtcatc aaatacaacg aattcagttt tgattcagct caccgctatt
     1021 ttgtttatgc cgcacttttc attgcgttgg taggattgtt ctttgaagta gtcggggatg
     1081 agcaactaag acaacacatc aacaaaaaaa cacgcacact tctgcaaaca ggcttatggt
     1141 caatcacacg tcatccaaac tatctcggtg aaatcctgat ttggatcgga ttgtatgcaa
     1201 gtggtattac gctgttcttc acggattcca tcaaccctta ttattatctg ttattaatca
     1261 tctcgcctat tttaatgagt acggtattaa tcaaaatatc aacgccatta ctggagaaaa
     1321 atatggagaa atatgatgga tgggaagaat acaccaaaag agttccaatg ctttttcctt
     1381 tcactaaaaa ataaggcagc ataggtatga ttaaaacacg ttattgaata aaatttgcga
     1441 agctgtgtgt gggggaggtg ctacttattc cttgcatgca gctttttggg cggttcttaa
     1501 ataatcgtgc tatagtatcc gtaataaatg gaggtgggat tgcatgtcct attcaatcgg
     1561 agagttttcg aaaatgatcg gtctacctgt gtcgacgctg cgctactatg aaaatgaagg
     1621 gctgttgaaa ccggatcgcg atgagaacaa cctgcgcgtc tatacagatc aggatttaaa
     1681 atgggtgact tttcttctgc atctgaaagg cacaggcatg tcgatggcgg aactgaagca
     1741 gtacaccatt tggcgtgccg aaggggatgc gacgatgctg gagcggatgg aactcttgga
     1801 aaagcgacga cgcttgttgg aaaaagatat ccaagcattg atgcacaatc ttgaggtgat
     1861 cgaaaataag atcgatttct acgaggagcg ccttgcaggc aataaagata gtatgatttc
     1921 ccaggttgaa gcagaaaagg aaaaaagtac gaaataaacc cgccaattgg tgggtttatt
     1981 tttatccctt gacttaatgt caaggttaag gtgtacgctc tgattgtatc agagagggga
     2041 aatgaatgat ggaacatctt aatcaacagc tattcaccgg ggagcgggct ctattcaaag
     2101 gaaaagacct gcagatcagc cattcagttt ttgcggatgg ggagtctccc ttgaaggaaa
     2161 gcaacaatat cgtactcgaa aacacgatct tccgctggaa atatccattg tggtatgcac
     2221 ggaacatcca ggcaaacaac atcactttag tggaaactgc gcgttccggc atttggtaca
     2281 caaatggcat cacgataacc gacagcatga tccaagcgcc caagatattc agacgagctg
     2341 ccgatatcaa gttggaacga gtggaaatgc caaatgccca agaaacgtta tggaactgca
     2401 atgatattac ct
//
LOCUS       DAOMUJ010000277         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12581_length_2401_cov_1.604433, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000277 DAOMUJ010000000
VERSION     DAOMUJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_2401_cov_1.604433"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLT73_07005"
     CDS             complement(<1..322)
                     /locus_tag="PLT73_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRM20163.1"
                     /translation="MSKILTLGEIMLRLSTDPGTLMTYSTNFTGHYGGGEANVGISLA
                     NFGHEVAFASKVPANPLGVAVRKHLKSYGICTALLRKGGERLGTYYLESGVGERAASV
                     VYDRA"
     gene            complement(579..>904)
                     /locus_tag="PLT73_07010"
     CDS             complement(579..>904)
                     /locus_tag="PLT73_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRM20164.1"
                     /translation="DTPNTITDRAVLKEEIRNVRMAGFATDDEENDLDVICIGAALVV
                     DNKLYGAFSVSTPKYRFDREKTIANKKEPAGRIGQDENFWLTIEEGRSCLKRGSFFGL
                     GWYDY"
     assembly_gap    905..914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<915..1399)
                     /locus_tag="PLT73_07015"
     CDS             complement(<915..1399)
                     /locus_tag="PLT73_07015"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM20165.1"
                     /translation="MEEEKKPYGTVLLKAAKIIDHLSNSKEAQSLAVIAANTGLSSPT
                     ALKILDTLLLIGYVEKDEQTKRFTLGSALIRHANNRLANLDIIKITEPYLRNLQAGLD
                     ETVHLGILDKDEVLYVNKLETQKAIANINSRIGNTNHLYSSAMGKAMLAVFSEEELEA
                     YL"
     gene            complement(1531..2169)
                     /locus_tag="PLT73_07020"
     CDS             complement(1531..2169)
                     /locus_tag="PLT73_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrrS family protein"
                     /protein_id="HRM20166.1"
                     /translation="MSENNQKPTRQELHRNHRRNNLLVVVGIIAAAFLGLVLLYNIFY
                     GNDETPSIAANNQTNSQNRIITESDDSSKTSDSAKDSSEKEQSSDTEEPEEDDEAETK
                     EVPSTDENVAKAYTGDWEPIGMSQTGEHSATDYTDGSADRIEIKQAVAAATGISADNM
                     IEWWIGNAGDQQVTATVSDTQKEKIARVQLKWVDGEGWQVTLVEELNEIPNN"
BASE COUNT          527 a          618 c          528 g          718 t
ORIGIN      
        1 gggcacggtc atagaccacc gaagcggcac gttcccccac gccactttcc aaataatagg
       61 tgccgaggcg ttcgccgccc tttctcagca aagcagtaca aattccgtag ctcttcagat
      121 gtttccggac cgcaaccccc aatggattgg ccggcacttt gcttgcgaaa gccacttcat
      181 gcccgaaatt cgccagactg atgccgacgt tcgcttcccc gccgccgtaa tgcccggtga
      241 aatttgtgga ataggtcatc aatgttcctg gatctgtaga taggcgcaac atgatttcac
      301 ccaatgtcaa aattttactc atcttttcgc acgctcccga ttatattgtt agttttcatg
      361 aacgcggtta cattcttctt tccggtatga tgcaaccgac ctgaaatgca ggacataaaa
      421 cccatttagt ttccatatag gaaatatagt tttcttttag gaaatacctt ggtttcatta
      481 tacggtttcc ttttggttat ttcaacacct atgtgagcga atttctattt tattgacatt
      541 tgaactgcag accgtcacct acaaacgcga acaccaaatc aataatcata ccatcctaaa
      601 ccaaaaaaac tgcctcgctt gagacagctc cgtccttctt caattgtcag ccaaaagttt
      661 tcgtcttgcc caattcggcc agcaggttct tttttgttcg cgatggtctt ctcgcgatcg
      721 aaacggtatt tcggcgtact gacgctgaac gcgccataga gtttgttgtc gaccacaagc
      781 gcagccccga tgcagatgac atccagatcg ttttcttcat catcggtcgc aaaaccggcc
      841 atcctaacgt tcctgatttc ctctttcaaa acagcgcgat ctgttatcgt gttcggtgtg
      901 tccgnnnnnn nnnnagatag gcctccaact cctcttcact gaacacagcc agcatcgcct
      961 tgcccatggc cgaactgtag agatggttgg tgttgccgat ccgggaattg atgttcgcaa
     1021 tcgctttttg tgtctccaat ttgttgacgt agaggacctc atccttgtcc aggatgccta
     1081 agtgaaccgt ttcatccaga ccggcctgca gattccgcag ataaggctcg gtgattttga
     1141 tgatgtccag attcgccagc cggttgttcg catggcggat cagcgcgctg cccaaggtaa
     1201 aacgcttcgt ctgctcatct ttttccacgt atccgatcaa aagcaatgtg tccagaattt
     1261 tcagtgccgt cggactggac agtcccgtgt tggctgcaat gacggccagg ctctgggctt
     1321 ctttgctgtt tgacagatga tcgatgatct tagcggcttt cagcaagact gttccgtaag
     1381 gttttttctc ttcttccatt ttttgacact cctcaactgt ttcctattca gaaacagttt
     1441 accccaaaac gaacatttcg tacagaatca tttctaaata agcaacgcga aaaaagacgc
     1501 cctgtaaaat agagcgcctt cgtgtgttga tcaattgttc ggtatttcgt tcagctcttc
     1561 cacaagcgta acttgccagc cttcgccgtc gacccatttg agttggacgc gcgcgatttt
     1621 ctctttctgt gtatccgaga ctgtggcagt gacttgctgg tcgccggcat tgccgatcca
     1681 ccattcaatc atattatcgg cgctgatgcc tgttgcggcg gcaacagctt gcttaatttc
     1741 gatgcggtca gcagatccgt cggtgtaatc ggtagcgctg tgctcaccgg tttgggacat
     1801 tccgatcggt tcccaatcgc cggtgtatgc ttttgcgacg ttctcatcgg ttgatggcac
     1861 ttcttttgtt tccgcctcgt catcttcttc cggctcttct gtatcgctac tttgctcttt
     1921 ttccgaagaa tctttggctg agtcggacgt tttgctggaa tcatccgatt cggtgatgat
     1981 cctgttttgt gaattcgttt gattattggc cgctatggat ggtgtttcat catttccgta
     2041 aaaaatgtta tagagcagca cgagccccaa aaatgcggct gcgatgattc ctacaacgac
     2101 aagcaggtta ttgcgtctat gatttcggtg taattcttgt ctggtcggtt tttgattgtt
     2161 ctctgacatg cagttgcctc cttctgaatg atacaagttt atcatgtttg cgatacggaa
     2221 acgttaaatc cagtttacaa ctctgtgtca caaaacctat ttgaccggac tgttcttctt
     2281 ggatgccttt atcctggcca cgagcccgaa aaggctgtca acaatgaaga ttgccagcgc
     2341 gatggcaccc gatatctgca gcgtgaggac cagatgctgc tgcgccaatg catactcttt
     2401 g
//
LOCUS       DAOMUJ010000278         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12676_length_2387_cov_1.629931, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000278 DAOMUJ010000000
VERSION     DAOMUJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12676_length_2387_cov_1.629931"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLT73_07025"
     CDS             complement(<1..707)
                     /locus_tag="PLT73_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20167.1"
                     /translation="MREHLVLIGNGMAGVRLVEEILERDPERYDITIIGKECYPNYNR
                     IMLSNVLQNKMTVDEIITNPYTWYEENNITLINEDPAVGVDTANKLVTSEKGLKIPFD
                     KLVFATGSHPFILPIPGSQKDGVVAFRTIDDTAEMMEAAGKHKKAVVIGGGLLGLEAA
                     KGLQLQGMDVTVVHLAKWLMETQLDERAGKLLQGDLEAQGLKFLLEHATTEILGDERV
                     TGLRFKDGTAIDTDLVV"
     gene            complement(1098..1547)
                     /locus_tag="PLT73_07030"
     CDS             complement(1098..1547)
                     /locus_tag="PLT73_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRM20168.1"
                     /translation="MYPMMVDISRRKVVVVGGGKIATRKLRELIRCGAQPTVVAPAVS
                     PEIQKWAEGGQALLVRRTYQSGDFDGATFIFICTDDPATNKAARSEVGPHQFLNDTTD
                     CSNSDFINLATLRQDDYLVAVSTYGNDPRKAKRLLREITEKINPTQA"
     gene            complement(1535..2224)
                     /locus_tag="PLT73_07035"
     CDS             complement(1535..2224)
                     /locus_tag="PLT73_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRM20169.1"
                     /translation="MNIGILYVLHGRKTSIPQKLLNIVKEFSDTLDLLQAIGMLEGQQ
                     QTLESAVTLLEEQAVEKIVVVPVLLFPATHAREDIPQRMKACAAVPFEICETLGSTRA
                     VYGWLKGRLQEVTERHPTLPVMLVVHGTTHYTEPAEQLERIAAALRSDLGREVFATNH
                     LGEPHYQAISGEHPYIVQRLFFTDGYLAKKIGVWFENNRPQDVLLEQLLDAEAVHAAL
                     KERLEDAGCIR"
     gene            complement(2235..>2387)
                     /locus_tag="PLT73_07040"
     CDS             complement(2235..>2387)
                     /locus_tag="PLT73_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRM20170.1"
                     /translation="LGYNNRDIAEKLFISVKTVEVHKANIRKKLEVDSYADLLRYSMK
                     NHLVDF"
BASE COUNT          558 a          631 c          538 g          660 t
ORIGIN      
        1 atgacgacaa gatcggtatc gatcgcggtg ccgtccttga agcgcaggcc agtgacgcgc
       61 tcgtcaccta atatttcggt tgttgcgtgt tccagcagga atttcagtcc ctgtgcctcg
      121 agatctccct gcaacaattt gcctgctctc tcatccaatt gggtttccat caaccatttg
      181 gccaaatgga caacggtgac gtccattccc tgcagctgta ggcctttcgc ggcttccaac
      241 ccaagcagac cgccgccgat gacgacagct ttcttgtgct tgcctgcggc ttccatcatt
      301 tcagccgtat catcgatggt tcgaaaggcg actacgccgt ctttctgcga accgggaatc
      361 ggtaaaatga atggatggga accagtggca aaaaccaatt tatcgaaagg gatcttcagc
      421 cctttttcgc ttgtgacaag cttgttcgct gtatcgactc cgaccgccgg atcttcgttg
      481 atcagcgtga tattgttctc ttcgtaccat gtatatggat tggtgatgat ttcatccacc
      541 gtcattttat tctgcagcac attggatagc atgatacgat tgtaattcgg gtagcattcc
      601 ttcccgatga tcgtgatatc atagcgttcc ggatctcttt ccagaatttc ttcaaccaaa
      661 cgaacccctg ccataccgtt tccgatcaag accaaatgtt ctctcatttg cgcaccctct
      721 tctattcgtt gttataaaat tcacaataaa ggatttgtga aatatttcat attagggaaa
      781 cacctatgtt ttgacgaatg ccataatttg gacaaaaacg ttttgcgcgt gcaatgtcgt
      841 acacaaaagc gattctaatg aacgagtcac tccctgagct aatctgccca actccggtta
      901 aagacattcc aaccggagtt atgatcactt atacggtgtc agttccgatt aagcaccctt
      961 cgtcggaggt agaggcgtta ttaccgtact ctacgccggt gaaggacagc tcgccggagg
     1021 taaggatatc gaaccatctg gtttccctcg atcagcccct tgtaagcaac ttccctcaca
     1081 acaaaaaaac tcaagcgcta ggcttgagtc ggatttattt tttcagttat ctcacgaagc
     1141 aaacgttttg ctttccgcgg atcattcccg taagtggaga cagccaccag gtagtcatcc
     1201 tgacgcaaag tagcaaggtt gatgaagtca ctgttgctgc aatcggtcgt gtcgttcaga
     1261 aactgatggg gtccgacctc cgatcttgcg gctttgtttg tagccggatc gtccgtgcag
     1321 atgaagatga atgttgcccc atcaaaatcg ccggattggt aagtccgcct gaccagtaat
     1381 gcctggccgc cttctgccca tttctggatt tccggactca cggcaggcgc gacgacggtc
     1441 ggctgagccc cgcaacggat cagttctcgt agtttccgcg tagcgatttt gccgcctccg
     1501 accacaacta ctttgcgtct gctgatgtcg accatcatcg gatacatccg gcatcctcca
     1561 atcgttcctt aagcgcagca tggacggctt ctgcatcaag caactgttcc agcagcacat
     1621 cttgaggacg attattttcg aaccagacac ctattttctt ggcaagatac ccatctgtaa
     1681 aaaacagccg ttgcacgatg taggggtgtt ctccagatat tgcttgataa tgcggttccc
     1741 ccagatgatt cgtagcgaag acttctcggc ctagatctga tctcaaggca gcggcaatcc
     1801 tttccagttg ttcagcaggt tcagtataat gggttgtgcc atgcacaacc agcatgaccg
     1861 gcaatgttgg atggcgctcg gtcacttcct gcaatcgacc tttcagccac ccgtagacag
     1921 cgcgcgtcga cccgagtgtc tcgcagattt caaacggaac tgcagcgcaa gctttcatgc
     1981 gctgcgggat atcttcccgt gcgtgcgtag ccgggaaaag cagtaccggg acaacaacaa
     2041 tcttttccac tgcctgttct tcaagcaacg tgactgcgga ttccaatgtc tgttgctgcc
     2101 cttccagcat gccgatcgct tgcagcagat ccaaggtgtc actgaactct ttgacgatat
     2161 tcaaaagttt ttgtgggatg ctggtctttc tgccgtgcaa aacatacaag attcctatat
     2221 tcatgctatt cctcctagaa atcgaccaaa tgatttttca tgctgtagcg caaaagatcg
     2281 gcataactgt cgacctccaa tttttttctg atatttgctt tatgcacttc taccgttttg
     2341 acagaaatga acagtttttc ggcaatgtcc ctgttgttgt agcccaa
//
LOCUS       DAOMUJ010000279         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12736_length_2379_cov_2.240103, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000279 DAOMUJ010000000
VERSION     DAOMUJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12736_length_2379_cov_2.240103"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="brnQ"
                     /locus_tag="PLT73_07045"
     CDS             <1..964
                     /gene="brnQ"
                     /locus_tag="PLT73_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HRM20171.1"
                     /translation="FFIAVLWFSLKPSEILKWVGKVLNPIFLVFLSVLILFGFFAPMG
                     AASEIPVDASYQTGAFFKGFLEGYNTMDALASLAFGIIVVTTIQGLGVTKTTTIAKDT
                     IKSGAISMLLMAVIYGSLVYLGATSRGIFEISDNGGIALAQISNEYFGIFGAVLFAVI
                     ITVACLKTAIGLITAISETFVHMFPNSLDYKQYVYLFTAISFGLANLGLSQIIAFSIP
                     VLMFLYPLAITLIFLSITSSLFNDRTIVYQMTTLFTLPFAIVDFLKALPQGIKDAVGL
                     DGFISWTDAAIPLSDIGMGWLIPSLLGFAIGLAISKNKQSINLK"
     gene            complement(1018..>2379)
                     /gene="ppk1"
                     /locus_tag="PLT73_07050"
     CDS             complement(1018..>2379)
                     /gene="ppk1"
                     /locus_tag="PLT73_07050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM20172.1"
                     /translation="NGMAVRLEIDTRDTIESLHGDVRFLQTELDLEDRDVYMIDGPLD
                     LTFLSKLTRYLAIASPEDVYPSFSPVTPSDLSGKNIFDVVEKKDVFLHHPYDSFDPVV
                     DFIKSAAEDEDTIAIKQTLYRVSKDSPIIEGLKAAAESGKQVTVLVELKARFDEENNV
                     QWAKELEEAGAHVLYGMTHLKTHSKITMVVKKQNNSIVRYVHLATGNYNDKTARLYTD
                     MAIFTANKEIGEDATNFFNYLSGYSDQPEYNHLHVSPFEIRDSFIEYIDDEIAAHKQS
                     GNGHIIAKMNSLTDKRVIMKLYEASQAGIRVDLIIRGICCLKPQVPGVSDNIHVRSIV
                     GRFLEHSRIYYFHHNGDENLFLSSADMMTRNMIKRIEIEFPILDKAIKKEILSLMDVY
                     LADNTKARELHPDGTYRHVRNDRPAVDAQKYFMELANKEKEIPILAEKDSWFKKIQRR
                     LKK"
BASE COUNT          559 a          551 c          562 g          707 t
ORIGIN      
        1 tttcttcata gctgtcttat ggttctccct gaagccctcg gaaatattga aatgggtagg
       61 taaggttctg aatccgattt ttctggtgtt tctttcggtt ttgattctct tcggattttt
      121 cgctccgatg ggcgcagcga gtgaaatacc agtagacgca agctaccaga ctggtgcatt
      181 cttcaaaggc tttttggaag ggtacaacac aatggacgca ctggcctccc tggctttcgg
      241 tatcatcgtc gtcacgacga tccaaggttt gggcgtcacc aaaacaacaa ccatcgcgaa
      301 ggacacgata aaatccggag cgatcagcat gttgttgatg gctgtcattt atggttcctt
      361 ggtttatttg ggtgcaacga gtcgcggcat atttgaaatc agcgacaacg gcggcatcgc
      421 cttggcacaa atctcgaacg agtacttcgg catcttcgga gcggttctgt ttgctgtcat
      481 catcaccgtt gcctgtctga aaacggcaat cgggctgata actgccatca gcgaaacgtt
      541 tgtgcacatg tttccgaatt cgttagacta taaacaatac gtctatcttt tcactgctat
      601 ttcctttggt ttggcgaatc tgggtttgag tcagattatc gccttctcga ttccggtatt
      661 gatgttcctg taccccttgg caattacctt gatcttcctg tcgatcacaa gcagcctgtt
      721 caatgaccgg acgattgtct accaaatgac gaccttgttc accttgccat tcgcaattgt
      781 tgattttctg aaagctttgc cgcaaggaat caaggatgct gtcggcctgg atggtttcat
      841 ctcttggaca gatgcagcga tcccgttatc cgatatcgga atgggttggc tgattccgtc
      901 gctgctcggt tttgcgattg gcttggcgat ttcaaaaaat aaacaatcaa ttaacttaaa
      961 atgaaagaac ggggctatct cagattgaga cagccccgtt cttttccgca tttttcgtta
     1021 ttttttgagt cttcgttgga tttttttgaa ccaggaatcc ttttcggcaa gaatgggaat
     1081 ctctttttcc ttattcgcca gttccatgaa atacttttgt gcatccacag caggcctatc
     1141 atttcggaca tggcggtaag tgccgtccgg atgcagttcg cgtgctttcg tgttgtcggc
     1201 aagatagacg tccatcaacg aaaggatctc tttcttgatg gctttgtcca gaatcggaaa
     1261 ttctatttcg atccgtttga tcatgttacg ggtcatcatg tctgcggaag aaaggaaaag
     1321 attctcatcg ccgttatgat ggaaatagta gatccggcta tgctccaaga aacggccgac
     1381 aatgctgcgg acatggatat tatcactgac gccaggaact tgaggcttca ggcagcagat
     1441 gccgcggatg atcaaatcca cccgtatgcc tgcttgactg gcttcgtata gtttcatgat
     1501 gacgcgtttg tcggtcaagg aattcatttt tgcgatgata tgcccattcc cggattgttt
     1561 atgcgccgcg atttcgtcgt cgatatattc aatgaatgaa tcacggattt cgaaaggtga
     1621 cacatgcaag tgattgtact caggctgatc ggaatagcca ctcaaatagt taaagaagtt
     1681 tgtcgcatcc tcgccgattt ccttgttagc cgtgaagatg gccatgtccg tataaagtct
     1741 ggcagtctta tcattgtaat tgccggtagc cagatggaca taacggacga tgctgttgtt
     1801 ttgctttttg accaccatcg tgattttgct gtgagtcttc aagtgggtca tgccatagag
     1861 cacatgcgca ccggcttctt ccaattcctt cgcccattgc acattgttct cttcgtcgaa
     1921 acgggctttc agctcgacca gtacagtgac ttgtttgccc gattctgctg cagccttcaa
     1981 cccttcgatg atcggtgaat ctttgctgac ccggtaaaga gtctgcttga tggcgattgt
     2041 gtcttcatcc tcagcggcac tcttgatgaa atcgacgacc ggatcgaacg agtcataggg
     2101 atgatgcaaa aaaacatcct tcttttcgac cacgtcaaaa atattttttc cggacagatc
     2161 gcttggcgtt accggagaaa agctagggta tacgtcttct ggagaagcaa tcgccagata
     2221 gcgggtcaat ttactgagga aagtaagatc caatggtcca tcgatcatat aaacatcacg
     2281 atcctcgaga tccagttcgg tctgcaaaaa gcgcacgtct ccgtgtaggg actcaatcgt
     2341 atcgcgcgtg tcaatttcaa ggcggacagc cataccatt
//
LOCUS       DAOMUJ010000280         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12745_length_2378_cov_2.036160, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000280 DAOMUJ010000000
VERSION     DAOMUJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12745_length_2378_cov_2.036160"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLT73_07055"
     CDS             complement(<1..186)
                     /locus_tag="PLT73_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20173.1"
                     /translation="MTRPLAMNAEDQVRFREAGYGRFYEIPLCEIKQLPIPESIKNKI
                     NAAEWLFFTSQAPVKSVL"
     gene            complement(201..1004)
                     /gene="hemC"
                     /locus_tag="PLT73_07060"
     CDS             complement(201..1004)
                     /gene="hemC"
                     /locus_tag="PLT73_07060"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRM20174.1"
                     /translation="MDPSVDIEVVGITTKGDRLVHVPLAQIGGKGVFVKEVEAQLLDG
                     TIDFAVHSLKDVPARMPEGLTIVATPKRANPFDCLIWNDDKPFSGTAVPMVIGTSSVR
                     RINQLKTAFPDMSFMPIRGNIETRLRKMKEEGLDGIVLAAAGLERMDYLKGLHTLMLE
                     PEVCVPAIGQGIMAVQCREGDKELMALLRGINDEAAAIACRTERFFLHRANGNCDLPI
                     GGYAHRLDDGQWEFLAFLAESTSKPGVRKRYVGDDPLELAKMAAAELLP"
     gene            complement(1124..1903)
                     /locus_tag="PLT73_07065"
     CDS             complement(1124..1903)
                     /locus_tag="PLT73_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRM20175.1"
                     /translation="MGALSPKELLEEAILRGKHKADYSIMTMATLGIMAGVFIGLAGV
                     AMIRTMGSMPVEWGTLVNLLGAMVFPVGLICLLLVGGELVTGNMLSVSLALFAKEISG
                     KSWLRNMAVVTLFNLMGALFVAYFFGYLSGTLQGAFIDRTIAVARGRIDVAPVQLFFS
                     SVACNILVCTGVYMNYAAKDFIGKIFGTFLPIMAFVIAGYQHVVANMFLIPAGIFAGG
                     ATWTEFMLNISIVWIGNLVGGGFFMGGLYFMAYRIGMRESR"
     gene            complement(1891..>2378)
                     /locus_tag="PLT73_07070"
     CDS             complement(1891..>2378)
                     /locus_tag="PLT73_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRM20176.1"
                     /translation="LYMGVEALDRFVEAALAQGMSARMPILITQWGTYGRQVKLQGTL
                     ADIRQKISETKIENPAMIILGEVVHAGERFDWFSDLPEKGESILYISTNRPDMDALLE
                     QIQKGADVWWMQVGETRDHRFDEVNKHYLAERHFDTIIFVDEAAKNSYEAEKVKPLWE
                     H"
BASE COUNT          586 a          687 c          498 g          607 t
ORIGIN      
        1 caagacactc tttacaggcg cttgactcgt gaagaaaagc cactcggccg cattgatttt
       61 atttttgatg gattccggta tcggtaactg tttgatttca cataaaggta tttcgtaaaa
      121 ccttccgtat cctgcctcac ggaagcggac ctgatcttcg gcattcatgg ctagaggcct
      181 ggtcatcaat accgctttct tcatggcaac aattcggcag cagccatttt cgccaactca
      241 agcggatcat ccccgacata acgtttcctt accccaggtt tgctggtcga ttcggccaag
      301 aaagccaaaa attcccattg gccatcatcc aatcgatgcg catagccgcc tatcggcaag
      361 tcgcagttgc cattagcccg atgcagaaag aaacgttctg tacggcatgc aattgctgca
      421 gcttcatcat tgattccgcg cagcaatgcc atcagttcct tatcgccctc acggcactgg
      481 acagccatga tcccttgtcc gatcgccggc acacacactt ctggttcgag cataagggta
      541 tgcaatccct tcagataatc catcctttca agtcctgctg ccgctaggac aataccgtcc
      601 aatccttctt ccttcatctt tcggagtcgg gtctcgatat taccgcggat cggcataaac
      661 gacatgtccg gaaatgcagt cttcaactgg ttgatcctgc gtacactgct tgtgcctatc
      721 accataggca cagctgtacc cgaaaaaggt ttgtcatcat tccagatcaa acaatcgaaa
      781 ggattcgccc ttttgggtgt ggcgacgatg gtcaatccct ccggcatacg ggctggcaca
      841 tccttgaggc tgtgcacagc aaagtcgatt gtcccgtcca acagttgggc ctcgacttcc
      901 ttcacaaaca cgcccttgcc tccgatttgc gccaagggca cgtgcaccag cctgtctcct
      961 ttggtggtga tgccgaccac ctcgatatct accgaaggat ccaatgacca gttcggtttg
     1021 tttcagggcc aatgcactac ttcgtgtccc tactcttagt gttttcatgc aacgtcctcc
     1081 cttctgtcac tattatagaa gactgacgct tgaaataaaa aggttatcta ctttctcgca
     1141 taccgattct gtaagccatg aaatagagtc cgcccatgaa gaatccgccg cccaccagat
     1201 ttccgatcca gacaatgctg atgttcagca tgaattccgt ccaagtcgcc cctccggcga
     1261 aaattcctgc cggaatcagg aacatgttcg ctacgacatg ctgataaccg gcaatcacaa
     1321 aagccatgat cggcagaaaa gtaccgaaaa ttttcccgat gaaatccttg gctgcatagt
     1381 tcatgtaaac gcctgtgcag accaggatat tgcaagcaac cgaagaaaag aacaattgaa
     1441 cgggtgccac atcgatacgg cctctggcga cagcaatcgt cctatcgata aacgctcctt
     1501 gcagcgtgcc ggacaagtaa ccaaagaaat aggctacaaa aagggctccc atcaagttga
     1561 aaagtgtgac gaccgccatg ttcctcaacc aggattttcc ggatatttct tttgcgaaca
     1621 acgcaagtga tacagacaac atattccctg tcaccaattc gccgcccacc aacaacaggc
     1681 aaatcaggcc gaccgggaag accatcgccc ccaacaagtt tacgagcgtg ccccactcaa
     1741 ctggcatcga tcccatagtg cggatcatcg caacacccgc cagtccgatg aagacaccgg
     1801 ccatgattcc caacgttgcc atcgtcatga tgctgtaatc agctttatgt ttgcctctta
     1861 gaatggcttc ttctaataat tctttcgggc tcaatgctcc cataatggct tcaccttttc
     1921 cgcttcatag ctattttttg ctgcttcatc aacaaagatg atggtatcga aatgccgctc
     1981 cgctaagtag tgcttgttta cctcatcaaa acggtgatcc cgcgtttcgc ctacttgcat
     2041 ccaccagaca tccgcgcctt tttgtatctg ctcaagcagt gcatccatgt caggtctgtt
     2101 tgttgaaata tacagtatcg attcgccttt ttccggcaag tccgaaaacc aatcaaagcg
     2161 ttccccggca tggacgactt cacccaggat gatcatggcc ggattctcga ttttggtttc
     2221 gctgattttt tgcctgatgt ccgctaatgt tccttgcaac tttacctgcc tgccgtatgt
     2281 tccccattgg gtgatcagga taggcattct cgctgacatc ccttgcgcca aggccgcttc
     2341 cacaaaacgg tcaagcgcct ccacacccat gtaaaggc
//
LOCUS       DAOMUJ010000281         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12826_length_2367_cov_2.537197, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000281 DAOMUJ010000000
VERSION     DAOMUJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12826_length_2367_cov_2.537197"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1062
                     /gene="pfkB"
                     /locus_tag="PLT73_07075"
     CDS             148..1062
                     /gene="pfkB"
                     /locus_tag="PLT73_07075"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRM20177.1"
                     /translation="MIYTITLNPTIDYIVTVPQLTLGHSHHMQNELITPGGKGIMVSR
                     VLKALGIPSIALGFRGGFTGDFVRDYMEELNILNQFTVIKERTRINLVLKSDQDTEIS
                     DYGPSATDEEVARFMKGFEDISTQDFVVMSGSKLPSMPKDFYEQLIRSLHKEGVPFAC
                     DITKEELRNSLQYHPLVVKPNQKEVGEFFGRTFTTWQEVVPYGKQLVELGAQHAIVSL
                     GGDGALLFTANEVIYAPPLSGKVANPVGSGDSMVAGFVGTFIRTGDPLEAFRVAVACG
                     TATAFSPDIATADEIEVQLDRVILEKIE"
     gene            complement(1137..1850)
                     /gene="budA"
                     /locus_tag="PLT73_07080"
     CDS             complement(1137..1850)
                     /gene="budA"
                     /locus_tag="PLT73_07080"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRM20178.1"
                     /translation="MGESTLYQHQTLGDLMAGVFDGTLTFEELLKHGDIGIGTFHDFE
                     GELILLDGIAYQAKADGSISKVGPSQTTPHAAVTFFEPDREYAIDRPMTLRKVRSDIV
                     KRVRSRNVFSVVKITGHFEYMHTRAVPKQQKPYPRLIEATRVQPEFETYDMQGTAIGI
                     YTPELFDGVAKGGFHCHFISDDRKFGGHILDYIVDEAKCEIQTIESITQHFAVQSDDF
                     MDKEIDYHNLAEEMAEAEG"
     gene            complement(2025..2339)
                     /gene="trxA"
                     /locus_tag="PLT73_07085"
     CDS             complement(2025..2339)
                     /gene="trxA"
                     /locus_tag="PLT73_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM20179.1"
                     /translation="MSTQVTDATWQSEVQEGLTLMDFWAPWCGPCRMLGPVLEEIEDE
                     MDNKVKIVKLNIDENQMTASQFGIMSIPTMVLFKDGQPVEKLTGYHPKDVLVDYLETK
                     LA"
BASE COUNT          530 a          630 c          554 g          653 t
ORIGIN      
        1 ttttcatgcg tttacgctcg ccgattttct cgttcatagc tgggagcctg cgcaaaaagg
       61 ctatcttttg agtttgcctg ccgacagggt tatactttgg ttgaaagcga aagtactgcg
      121 tgccatccca gactggagga attatatatg atttacacga tcacactgaa tccgaccatc
      181 gattatatcg tcactgtccc tcaattgact ttgggccatt cccatcatat gcagaatgaa
      241 ctgatcacgc ccggtgggaa aggcatcatg gtttcgcgcg tcctgaaagc actcggaatc
      301 ccctcgatcg cgctgggctt ccgcggcggg ttcaccggtg actttgtgcg cgactacatg
      361 gaggaactga atatcctcaa ccagttcacc gtgatcaagg agcgcacccg catcaatctc
      421 gtcctgaagt cggaccagga taccgaaatc agcgactacg gtcccagcgc aaccgatgag
      481 gaagtcgccc gtttcatgaa aggttttgag gacatctcaa cgcaggactt tgtagtgatg
      541 tcgggcagca agctcccttc catgccgaag gatttttatg aacagctgat ccgctccctt
      601 cataaagaag gcgtgccttt tgcttgcgac atcactaagg aggaattgcg gaacagtctg
      661 cagtaccatc ccttggttgt gaaacccaac cagaaggaag tcggcgaatt tttcggacgg
      721 actttcacca cttggcagga ggttgtccct tacggcaaac aactggttga attgggcgcg
      781 cagcatgcca tcgtttcctt gggcggggat ggcgctttgc tcttcacagc gaatgaagtc
      841 atctatgcgc ctcctttatc cgggaaagtc gccaatccgg tcggctccgg ggattccatg
      901 gtggccgggt ttgtagggac ctttatccgt actggggacc ccttggaagc tttccgggta
      961 gccgtcgctt gcgggactgc cacagccttc tcacccgata tcgcaaccgc cgatgagatt
     1021 gaagtgcagt tggaccgcgt tattttggaa aaaatcgaat aagtccacaa tcttaaaaag
     1081 cgttggataa tcccggtctc cggaggttat ccaacgcttt ttcccatttt tctttatcag
     1141 ccttctgctt cggccatttc ttccgctaag ttatggtagt cgatttcttt gtccataaaa
     1201 tcatcggatt ggacggcgaa gtgttgtgtg atgctttcga tagtttggat ttcgcatttt
     1261 gcttcatcaa cgatgtaatc aaggatatgc ccaccgaact tgcggtcgtc actgatgaaa
     1321 tgacaatgaa agcctccttt ggcgactcca tcgaacaatt ccggcgtata gatgccgatt
     1381 gctgtcccct gcatgtcata ggtctcgaac tccggctgga cacgcgtcgc ttcgatcaag
     1441 cgcggataag gcttctgttg cttcggcact gcccgcgtat gcatatattc gaaatgaccc
     1501 gttattttga cgaccgagaa cacattccgg ctgcgcacgc gcttgacgat gtccgagcgt
     1561 acttttctca acgtcatcgg cctgtcgata gcgtattcgc ggtccggttc gaaaaatgtc
     1621 accgccgcat gcggtgttgt ctgggatgga cccactttcg aaatcgatcc gtccgccttc
     1681 gcttgatagg cgatgccgtc aagcaggatc agttcccctt caaaatcatg gaaagtcccg
     1741 atgccgatat ccccatgctt caacagttct tcgaatgtca gagttccgtc gaataccccc
     1801 gccatcaaat cacccagcgt ctgatgctga tacaatgtac tttcgcccat ctgcttccgc
     1861 ccttctgtgc ctttgtgcaa tcatttattt ttctgtctga tcatgatagc ccattatatc
     1921 acagccagaa ggcggcaaac aatcgcgtat tcatgggtat caggtgctgg gcgaccagaa
     1981 tagggccgcc ccgaaaatcg ggacagccct ccattcataa tatattacgc tagtttagtc
     2041 tccaggtaat caaccaaaac atctttcgga tggtaccctg tcagtttctc aaccggttgt
     2101 ccgtctttga acaacaccat tgtcggaatg ctcatgatac cgaattgaga agctgtcatt
     2161 tggttctcgt cgatattcag tttgacaatt ttcactttat tgtccatctc atcttcgatt
     2221 tcttccaaaa ctggtcctag catgcggcaa ggtccgcacc aaggcgccca aaaatccatc
     2281 aatgtcaagc cttcttgtac ttcactctgc catgttgcgt ctgttacttg tgtgctcatt
     2341 ttttatctgt ccttccttaa actgtat
//
LOCUS       DAOMUJ010000282         2361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_12868_length_2361_cov_2.510408, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000282 DAOMUJ010000000
VERSION     DAOMUJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_2361_cov_2.510408"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /gene="argS"
                     /locus_tag="PLT73_07090"
     CDS             <1..1062
                     /gene="argS"
                     /locus_tag="PLT73_07090"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM20180.1"
                     /translation="AWFKKLEDGDAEALHLWEWFRSESLQEFMKIYDMLGITFDSFNG
                     EAFYNDKMDEVVELLDKAGILTQDRGATIVDLEKYNLNPALIKKSDGATLYITRDLAA
                     AIYRKRNYDFAMSLYAVGNEQSNHFKQLKAVLKELGYDWAENMHHIPFGLITQGGKKL
                     STRQGKVILLEEVLNEATELSLKQINEKNPTLENKEEVAKAVGIGAVVFHDLKNDRLN
                     NFDFDLEEVVQFEGETGPYVQYTNARGLSILRKAAVELDTTEAADLGLDDAYAWEVVK
                     LLNSFPEIIQQAYEKFEPSVIAKYTLHLSQAFNKYYGNTKVLVEDDKRNARLALVQSV
                     ATVLQEGLRLLGVQSPEKM"
     gene            1483..2361
                     /locus_tag="PLT73_07095"
                     /pseudo
     CDS             1483..2361
                     /locus_tag="PLT73_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
BASE COUNT          635 a          541 c          582 g          603 t
ORIGIN      
        1 gcttggttca agaaattgga agacggcgat gcagaagctc tgcatctttg ggagtggttc
       61 cgttccgaat cgctgcagga attcatgaaa atctatgata tgttgggaat cacgttcgat
      121 tccttcaacg gcgaagcttt ctacaacgac aagatggatg aagtcgttga gctgctggac
      181 aaagccggca tcctgaccca ggaccgcggt gcaaccatcg tcgatctgga aaaatacaat
      241 ctgaatccgg ctttgatcaa gaaatcggac ggcgcaacgc tgtacatcac ccgtgacttg
      301 gctgcggcga tctaccgcaa acgcaactat gattttgcga tgtccttgta cgcagtcggc
      361 aatgagcaaa gcaaccattt caaacaactg aaagctgttc ttaaagaact cggctatgat
      421 tgggcagaaa acatgcacca catcccattc ggtctgatca cacaaggcgg caaaaaattg
      481 tcgacgcgcc aagggaaagt catcctgctt gaggaagtcc tgaatgaagc gaccgagctt
      541 tccttgaaac agataaacga gaagaatccg acattggaaa ataaagaaga agtcgccaaa
      601 gcagtcggca tcggtgccgt cgttttccac gacctgaaaa atgaccgtct gaacaatttc
      661 gacttcgatt tggaagaagt cgtgcaattc gaaggcgaaa caggcccata cgtacaatat
      721 acaaacgcac gtggcttgag catcctacgc aaagcggcag ttgaactgga cacgaccgaa
      781 gctgctgatc ttggtttgga tgacgcgtac gcatgggaag tcgtgaaact gttgaacagt
      841 ttccctgaaa tcatccaaca agcctacgaa aaattcgagc catccgtgat cgcgaaatac
      901 acgctccact tgtcccaagc attcaataaa tactacggca acacaaaagt gcttgtcgaa
      961 gacgacaaac gcaacgcccg tctggctctt gtacaatccg tagccacagt cctgcaagaa
     1021 ggcctaagac tcctaggagt ccaatcccca gaaaaaatgt agagcgagat gaatcccgct
     1081 ctaggaatcg agcactaacg aaatttagga acagccagcc cggttttggc tggatgggcc
     1141 taaatagcgt taggcggaga ttactgggat tcagaacgcg ttttcgtagg atacgatgaa
     1201 ttgctcaccc cctgcaattc agagttcgac tatgtaggat acgacaaaaa ccaaaatcgc
     1261 ctaaaattat gcacatagtt ttaggttttt tgattttcat tgaacaatta aaaaagaaag
     1321 aagggataat ccgtggatat ctacaggaag ctcatggctg atgaacgtgt catttacctc
     1381 atgaaaggaa ccctggtggg tttatcagcc ggttttattg ttagtctatt cagattagga
     1441 attgaattta ttttagaaac agtgatcgaa ggctatcatt tcatgcagga acagccaatc
     1501 tggctcatcc cttgggtaat tttttcagtg acagccgcgc tcatcgtcgg aaaacttgtt
     1561 aaaagcgaac ctaacatcaa gggcagcggc attccgcaag ttgaagggca agtacagggg
     1621 ctcatcagct taaactggtg gccagtactc tggaaaaaat tcatcggcgg tctactggcg
     1681 atcggatccg gcctttttta ggaagggagg gcccatccat ccaactggga tccgcagtgg
     1741 ggcaaggcat cagcaatctc acaaaggggg atgatgtcga agaaaaaatc cttctttcga
     1801 gcggtgccgg tgccggtttg gccgcagctt tcaatgctcc tttggcagga ctgatgtttg
     1861 tcctggagga ggtccatcat aatttttctc ccttgttggc catcacgact ttctcttcgg
     1921 cgcttgtggc aaattttgtg tcccttaata tcttcgggtt gactccggcg ttggatatcg
     1981 gcatgatgaa cacgatcccg atcgcctact atggtttggt tattggttta ggcatctttc
     2041 tgggattgaa tggctggctc tacaccaaag tagtcttggt attgcctaaa ctgtatggca
     2101 ggttgccatt catcccagct cggtataatg caatcttgcc attcctactg gtcattccga
     2161 taggctattt tcttccccag gtcatcggag gtggcagcga gctgatcttg catttgagca
     2221 gatggcaact ctcgttaggg atattgattg gcctctgggt tttgcgcttt gtattttcga
     2281 tgatccctta tggagcgaat gttcctggcg gtatttttct gccgatctta tccttggggg
     2341 caatattggg cgccatctat g
//
LOCUS       DAOMUJ010000283         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13033_length_2338_cov_1.661410, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000283 DAOMUJ010000000
VERSION     DAOMUJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13033_length_2338_cov_1.661410"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PLT73_07100"
     CDS             <1..1261
                     /locus_tag="PLT73_07100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM20181.1"
                     /translation="QVISFIAGSLGEGDTKPYLVICPSSVLYNWQKEFQQFAPDIKTR
                     LITGMKSERRDTIATAIAENIPVWITSYPLLQRDEDLYEGLAFRTVILDEAQSVKNAT
                     AKTTKAASALNANNKIALSGTPVENALSELYTIFSIAQPGLFGTKKDFKQMDPAQIAK
                     KVKPFILRRLKADVLKDLPPKIESISYIEMTEKQKTVYLSQLQLMRQETKEMLDADTL
                     NENRIKILAGLTRLRQICCDPALVMADYTGGSGKLDRLMEYLAEAKENGRRVVLFSQF
                     TQMLAIIREKLDETSEAYFYLDGQTPNQDRLALTTRFNEGEKDLFLISLKAGGTGLNL
                     TGGDTVILYDSWWNPAVESQATDRVHRFGQKKVVQVVRLIATGTIEERINELQAKKRE
                     MIDSVIQAGGQSVTSLSKEELMQLLEI"
     assembly_gap    1362..1371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1372..2111
                     /locus_tag="PLT73_07105"
     CDS             <1372..2111
                     /locus_tag="PLT73_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM20182.1"
                     /translation="TAVLAGQIMLESNAETYRVEDTVTRILKKTGLQMTEALALTTGL
                     VATLDSPNMHAITVVKRITERTTNLNRVSRVNAVSRNFVEDRLTIQEAYDALQNIDEI
                     QYSRRQKSFALIGFIQLFIFLMGGTFYDFLAMLPVSATVSFVLHTAVKWKIRPFIQNL
                     VSSFVIAVMTAILSELLTFPIQPDTIIISAIMPLLPGTVLTNGIRDTFRGDYMSGAAK
                     ILEAFVIAIFIAIGIGAGLVVGGEVIR"
     gene            2108..>2338
                     /locus_tag="PLT73_07110"
     CDS             2108..>2338
                     /locus_tag="PLT73_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM20183.1"
                     /translation="MSVLQIALEMVVAFVAVFFIAVTLEAPKRTLRYGALAGGLGWGV
                     YLVGLLFMDIVAATFMASLFIAWIAHLFARYLK"
BASE COUNT          569 a          588 c          635 g          536 t
ORIGIN      
        1 acaggtcatc agtttcatcg ctggttcgct cggggaaggc gacaccaaac cgtatttggt
       61 catctgtcct tccagcgtgc tctacaattg gcaaaaggaa ttccaacagt tcgcgccaga
      121 catcaaaacc cggctcatca ccggcatgaa gagcgaaaga agggacacga tcgcaactgc
      181 aatcgcagaa aatattccgg tatggatcac ctcctatccc ttgcttcagc gcgatgagga
      241 tctttatgag gggctggctt tccgtaccgt catcctcgat gaagcccaga gcgtcaaaaa
      301 cgccacagcg aagacgacaa aagcggcttc cgcgctgaac gccaacaaca aaatcgcttt
      361 gagtgggacg cctgtcgaaa acgcgttgtc cgaactctac accattttca gcatcgcgca
      421 gcccggactg ttcgggacga aaaaggactt caaacagatg gatccggcgc agattgccaa
      481 gaaagtcaaa ccgttcatcc tgcgcagact gaaagctgat gtgctgaagg atctgccgcc
      541 gaaaatcgaa tcgatttcct acatcgaaat gaccgaaaaa caaaagaccg tctacttgtc
      601 gcagctgcag ttgatgcgcc aggaaacgaa agagatgctg gatgcggata cgctgaacga
      661 gaaccggatc aaaatcttgg cgggcctgac acgcctgcgc caaatctgtt gcgatccggc
      721 tttggtcatg gccgactaca caggtggttc cgggaaattg gacaggctga tggaatattt
      781 ggcggaagcc aaagagaacg gcagacgcgt cgtgttgttc tcccagttca cgcaaatgct
      841 ggcaatcatc cgtgagaaac tggatgaaac gagcgaagcc tatttctatc tcgatggcca
      901 gacgccgaat caggaccggc tggctttgac gacccgcttc aacgaaggcg aaaaggatct
      961 tttcctgatt tccctgaagg ctggcgggac cggccttaac ttgactggag gggataccgt
     1021 catcctttat gattcctggt ggaaccctgc ggtcgagagc caggcgaccg accgcgtgca
     1081 ccgtttcggc cagaaaaagg tcgtgcaggt cgttcgcctg atcgcgaccg gtacaattga
     1141 ggaacggatc aatgaattgc aggcgaaaaa acgcgaaatg atcgattccg tcatccaggc
     1201 tggcggccaa tcggtcacct ccttgtcgaa ggaagaattg atgcagctgc tggaaatata
     1261 gatcttgcta taatgaatcg gcgtgggctg ctcgcaggct gcgcacaagc caataatgtt
     1321 ttgagaagga agggggattt ggatgacgga tacacctaat cnnnnnnnnn natacagcgg
     1381 tcctggccgg acaaatcatg cttgaaagca acgcggaaac gtaccgtgtc gaggatacgg
     1441 tgacacgcat cttgaagaag accgggctgc agatgaccga agcattggct ttgaccaccg
     1501 ggctggtggc tacgctcgac agtccgaata tgcatgcgat tacggtcgtc aaacggatta
     1561 ccgaacgcac gacgaacctg aacagggtgt cccgtgtcaa tgccgtttcg cggaatttcg
     1621 tcgaagacag attgacgatc caagaggctt acgatgcgct gcagaacatc gacgaaatcc
     1681 aatacagcag gcgtcagaaa agctttgctc tgatcggctt tatccaattg ttcatctttt
     1741 tgatgggcgg caccttttac gactttttgg cgatgcttcc ggtcagcgcc acagtttcgt
     1801 ttgtactgca caccgcggtc aaatggaaaa tccgcccatt catccaaaac ctggtctcca
     1861 gtttcgtaat tgctgtgatg acggccattt tgtccgagct gttgaccttt ccgattcaac
     1921 cggatacgat catcatcagt gccatcatgc cattgttgcc gggaacggta ttgacgaacg
     1981 gcatccgtga tactttccgc ggggactaca tgtcgggagc tgcaaaaata ttggaggcct
     2041 ttgtgatcgc gatattcatt gcgatcggca tcggtgccgg cttggtcgtc ggcggcgagg
     2101 tgatccggtg agtgtgctgc aaattgcttt ggaaatggtc gttgctttcg tggccgtttt
     2161 ctttatcgcc gtcactttgg aagcaccaaa acggactttg cgttatggcg ctttggcagg
     2221 tgggttaggt tggggtgtct accttgtcgg cctattgttc atggatatcg tcgcagccac
     2281 ttttatggcc agtctgttca tcgcttggat tgcgcacctg ttcgcgcgtt accttaag
//
LOCUS       DAOMUJ010000284         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13037_length_2337_cov_2.234882, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000284 DAOMUJ010000000
VERSION     DAOMUJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13037_length_2337_cov_2.234882"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..747)
                     /locus_tag="PLT73_07115"
     CDS             complement(373..747)
                     /locus_tag="PLT73_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20184.1"
                     /translation="MEALTEADKAESMKSFRSTIQKSENALSSMTNKGANTTLVAKRL
                     QALRIGLAVLEQVWDEKHHPYTHEELAEARILLAGLLPSIEGIYAKSKPSSPQKTLLE
                     RRIKSLELAIQAMNDRNDKKNV"
     gene            complement(731..2059)
                     /locus_tag="PLT73_07120"
     CDS             complement(731..2059)
                     /locus_tag="PLT73_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM20185.1"
                     /translation="MEKSLVKDFSKYVSLNILGMIGVSFYILADTYYIAKALGATGIA
                     ALNFAIPVFSVILGIGLMIGIGGAARFGILKSRNKHKEAEAVFAGALKLGTLAGLLFA
                     VLGLFGSKALSSILGADAVTLPLTHAYMATIMVFAPFFILNNTVLAFVRNDNNPRLAM
                     LAMIGGSVSNVILDYIFLFPLKMGMFGAAFATSLAPIISLGILSIHFAKKKSRPAILK
                     HKLTRPAIKDILNLGSSTFIMEVSSAVVLTTFNLLILGLEGNKGVAAYGIVANIAFVA
                     VAIFTGLGQGVQPLISKYYGLKDRKTVRRVNRYALLTAGFIAALIYLGTFFNSEALIG
                     LFNNENDPVILKLADSGMKIYFLGFFFVGINIVTAMILSATEKGRAAFVLSVLRGLAV
                     IVPMAILLGSLWGMTGIWSSFVLSELIVAGFALYLANATEKGAGAYGSTD"
BASE COUNT          640 a          572 c          488 g          637 t
ORIGIN      
        1 tatattaatc atgatgttcc tccaagtttc caattctaat tgttaattct atttaagtca
       61 ttagaaatgc tgacttatcg tcacttagtt attggtaaaa aagtccattc aaagctcttc
      121 caagccctct aattgactat atatccgagt acttgcagct tgtttactgt tcttttgcat
      181 gcaagtatat gtagtatatt atatcactaa aaccattcaa cttttgtact tattatcttt
      241 atattctaga aggtatactc ttcttctgat tgatcgttta ttgtttccag agaaatatag
      301 atttattcaa aaaaatcctc gacataggct taaaataacc ttttcgagga gtattgttct
      361 atggatattt tattagacgt tttttttgtc attcctatca ttcatcgcct gaatggccaa
      421 ttccaacgac ttgatccttc tttcaagaag ggttttctgc ggactgctcg gcttcgactt
      481 ggcgtagatg ccttcgattg agggcagcaa acccgcaagc aggatgcggg cttccgccaa
      541 ttcctcgtgg gtgtaaggat ggtgtttttc atcccaaact tgttccagca cagccaagcc
      601 gatgcggagt gcctggagcc gtttcgccac caaagtcgtg ttggcgcctt tattggtcat
      661 gctgctcaac gcattttcgg atttttggat cgttgatcgg aaggatttca ttgattcagc
      721 tttgtctgct tcagtcagtg cttccatatg caccagctcc cttttcggtg gcgttcgcta
      781 aatagagcgc aaatccggca acaatcagct cggaaagcac aaatgatgac caaattcctg
      841 tcattcccca aaggctaccc aataagattg ccataggtac gataaccgcc agtcccctca
      901 gaaccgaaag gacaaaagct gcccgtccct tttcggttgc gctgaggatc atcgccgtca
      961 cgatattgat tccgacaaag aaaaatccaa gaaaatagat tttcattccg ctgtcagcca
     1021 actttagtat caccggatca ttttcattgt tgaacaggcc gatcagcgct tccgagttga
     1081 agaaggtccc caggtaaatc aaggcagcga tgaaacccgc cgtcaacagt gcatagcggt
     1141 tcacgcgccg gaccgtcttg cgatccttca ggccataata tttgctgatg agcggttgta
     1201 caccctgccc cagcccagta aaaatcgcga ccgccacaaa ggcaatattt gcgacaatcc
     1261 cataggcggc aacccctttg tttccctcga gccccaaaat caacaagttg aaggtcgtca
     1321 gcacgactgc cgaggacact tccattatga aagtggaaga tcccagattg agaatgtcct
     1381 tgattgccgg acgcgtcaac ttgtgcttca aaatagcggg ccgggatttc ttttttgcga
     1441 aatggatcga gaggatgccc agactgatga tcggggctaa gcttgtcgca aaagcggcac
     1501 caaacatccc catttttagt gggaaaagga atatgtaatc caaaatgaca ttcgaaacac
     1561 tgccgccaat catcgcaagc atagccagtc tgggattgtt gtcgttccgg acgaaagcca
     1621 ggaccgtatt gttcaggatg aaaaatggtg caaacaccat aatcgtggcc atataggcat
     1681 gtgtcaaagg caatgtgact gcatcggcac ctaaaatcga tgataatgcc ttcgaaccga
     1741 acaatccgag cacagcgaac aacaagcctg ccagagtgcc aagcttaagc gcccctgcaa
     1801 agaccgcctc tgcttctttg tgtttgttcc gggatttcag gataccgaaa cgggctgctc
     1861 cgccgatgcc gatcatcaga ccgattccga ggatgacact gaaaaccggg atagcaaaat
     1921 tcaacgccgc gatcccggta gcccccaaag cttttgcgat ataatacgtg tcggccaaaa
     1981 tatagaacga cacgccgatc atgcccaaaa tattcagact gacatacttc gaaaagtcct
     2041 tcactagtga tttttccatg ctgctgtctc cttccaaaat aaaaaaggtg tcccggaaac
     2101 actccttcaa agacctacgg aatgtttctg aacaccttga actctctgcc cggtggcaaa
     2161 aagagggtta tctattagat tgatatcact tgattataat ttttcggaga aaatttgtca
     2221 atgtcgtatt caaacgggat gaatggatac cggattagtt cagcattaat aaagtaacca
     2281 tccttattcc cccttgcact acttgcctag actactcccc aagcggttga tactccg
//
LOCUS       DAOMUJ010000285         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13050_length_2336_cov_1.743972, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000285 DAOMUJ010000000
VERSION     DAOMUJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13050_length_2336_cov_1.743972"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1626)
                     /locus_tag="PLT73_07125"
     CDS             complement(94..1626)
                     /locus_tag="PLT73_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRM20186.1"
                     /translation="MDKAALLALQNGSDVRGIAISTEKHEATLTADKVTRIGYGFVEW
                     LQKDKNINLNSEKKVKIAIGQDSRLSGGDIKTALMAGMAPFAIEIVDVGLSTTPAMFM
                     STQYKDYATDAAIMITASHLPFEYNGLKFFTKTGGAEHEDIEAILEYAYEIPEAFAEE
                     EQTAVVTEMDLLSTYAADLQEKIRKGIASAENPEKPLTGRHIIVDAGNGAGGFFVERV
                     LQPLGADTSGSQFLDPDGTFPNHVPNPDNKAAMKSIQDAVIANKADLGIIFDTDVDRS
                     AIVDSDGKAFNRNNLIALISAVLLNAEPSATIVTNSATSSHLDTFITGLGGVQDRYLT
                     GYRNVINRGIELNEAGINAVLAIETSGHAALKENYFLDDGAYLIAKLLIADAQLSTEG
                     KRLGDLIATLGQPAETMEYRFTIIEEPIFEVGNAIIKDFAAFIAATEDMALVTDHLEG
                     VRVNLSGQYGEGWFILRLSLHEPLLVWTIESDAVGKLPLIAQTVLPFFKGRPELDTGH
                     LH"
     assembly_gap    1748..1757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1758..2266)
                     /gene="trmL"
                     /locus_tag="PLT73_07130"
     CDS             complement(<1758..2266)
                     /gene="trmL"
                     /locus_tag="PLT73_07130"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRM20187.1"
                     /translation="MTNHIVLFEPQIPANTGNIARTCAATNTTLHLIEPLGFSTDDKQ
                     LKRAGLDYWHDVNIIHHADLAAFLDYLGDRQLYLITKFADRIYSEADFTKDEDTFFIF
                     GKETTGLPESFMQENAEKCLRIPMNDEHVRSLNLSNTACMVVYEALRQQGFPNLELTH
                     TYENDKLKG"
BASE COUNT          538 a          608 c          545 g          635 t
ORIGIN      
        1 aaaatgaaga acccgaagta accgaataag caaaaaaaac tttgtgacca ggcttttgaa
       61 cgcctgtcgc aaagtttttt ttgatgagat ttcttaatga agatgccctg tatccaactc
      121 cggacgccct ttgaagaaag gcagcacggt ctgtgcgatc aacggcagtt tgccgactgc
      181 atcgctttcg attgtccata ccaacaacgg ttcatgaagg ctcaaccgca ggatgaacca
      241 gccttccccg tactggccac tcagattcac gcgcacgccc tcaagatgat ctgtaaccaa
      301 tgccatgtcc tcggtcgcag cgatgaaagc cgcaaaatcc ttgatgatgg cattgcccac
      361 ttcaaaaatc ggttcttcga tgatcgtaaa gcgatactcc atcgtttccg ccggttgacc
      421 cagtgtcgcg atcaaatcgc ccaagcgttt tccttccgtt gacaactgcg catctgcgat
      481 cagcagcttt gcgatcaggt aggcaccgtc atccaggaaa taattttctt tcagcgccgc
      541 atgtccgctc gtttcaatcg ccaacactgc attgatgccc gcttcattca attcgatgcc
      601 gcgattgatg acattccggt agccggtcag ataacggtcc tggaccccgc caaggcccgt
      661 gatgaatgta tcaagatggg aagaagtcgc ggaatttgtt acgattgtcg cacttggctc
      721 tgcattcaac aacaccgccg aaatcaacgc aatcagattg ttgcggttga acgcttttcc
      781 gtcgctgtcg acgatcgcgg aacggtccac atccgtgtcg aagatgatcc caagatcggc
      841 cttgtttgcg atcaccgcat cctggatgct cttcattgcc gctttgttgt ccggattcgg
      901 gacgtggttc gggaaagtgc cgtccggatc caggaattgg ctaccggaag tgtcggcccc
      961 caaaggctgc agcacccgtt ccacaaagaa tccgcccgct ccgttaccgg cgtccacgat
     1021 gatgtggcgt ccagtcaatg gtttctcagg attttccgct gaagcgatgc ctttgcggat
     1081 tttttcctgg agatcggctg cataagtcga aagtagatcc atctcggtca caactgcggt
     1141 ttgttcttct tcagcgaatg cttccggtat ttcgtaggcg tattccaaaa ttgcctcgat
     1201 gtcctcgtgc tccgctccac ctgttttagt gaagaatttc agtccattgt attcaaaagg
     1261 aagatggctg gcggtgatca tgatcgctgc atccgtcgcg taatctttgt actgcgtcga
     1321 catgaacatc gccggtgtag tggaaaggcc cacatcgacg atttcgatgg caaaaggcgc
     1381 catcccagcc atcagtgcag ttttgatgtc tcctccggat aagcggctat cctgtccgat
     1441 cgcaattttg actttctttt cgctgttcag attgatattt ttatcttttt gcaaccactc
     1501 cacgaaaccg taaccgatac gcgtcacttt gtccgccgta agcgtcgctt catgtttttc
     1561 cgtcgaaatc gcaatcccgc ggacatccga cccattctgt aaggccaata atgcagcttt
     1621 atccattatt ccatcccctt ttcaatttta cgttacatcc attataccta ttcactggaa
     1681 ataaaaaaag aacggccgaa gctttttcgc ttttggccat tctatttgtg aaattatcaa
     1741 ctgggtgnnn nnnnnnntag cccttcaatt tatcgttctc atacgtgtga gtcaattcca
     1801 gattcgggaa tccttgctga cgcaacgcct cgtagaccac catgcaggct gtgttcgaca
     1861 ggttcaggga gcgcacatgc tcatcgttca tcggaatgcg caggcatttt tcagcgtttt
     1921 cctgcatgaa agattcaggt aggcccgtcg tctctttgcc gaagatgaag aaggtgtcct
     1981 cgtccttcgt gaagtcggcc tcgctgtaga tgcggtcggc gaacttggtg atcagataga
     2041 gctggcgatc gcccaaatag tcgagaaaag ccgccagatc cgcatgatgg atgatgttca
     2101 cgtcatgcca ataatcaagt ccggcacgtt tcagctgctt atcatcggtc gaaaagccca
     2161 acggttcgat cagatgcaaa gtagtgttgg ttgcagcgca agtgcgcgcg atattgcctg
     2221 tattcgcagg gatctgcggt tcaaatagta cgatatggtt ggtcataagt gagactcctt
     2281 tattctgtta gattaatctg cttctttgat atcaagtatt ttcagataag ccttct
//
LOCUS       DAOMUJ010000286         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13147_length_2320_cov_2.874614, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000286 DAOMUJ010000000
VERSION     DAOMUJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13147_length_2320_cov_2.874614"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..711
                     /gene="rimI"
                     /locus_tag="PLT73_07135"
     CDS             94..711
                     /gene="rimI"
                     /locus_tag="PLT73_07135"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRM20188.1"
                     /translation="MREWIHRFKSMYKEIRFDVLAKIHSKRVLSNEKTYLLRNQLPVR
                     LMVGEKQHIPDILYIERMSYDGATPWGRTALENDMLYNPKSLYYVLYEGRMPIGFLGA
                     RLDGKDIHITNLAVVPEFQQIGAATLLLRVLRSFAEEKSATSISLEVRISNHRAKALY
                     TKMGFLPEQIKQHYYHGDGEDALDMVWPLAGKQSEEKEDVHATHV"
     gene            695..1195
                     /gene="rimI"
                     /locus_tag="PLT73_07140"
     CDS             695..1195
                     /gene="rimI"
                     /locus_tag="PLT73_07140"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRM20189.1"
                     /translation="MQRTFEICDLKTDAGKLDQHAQTLYELTMLANEGHSSWKVSSFL
                     SELKDNHSIYVGYMEENKLVGYICCMAVVDEASINNFAVAPDHQAKGIGTKLLQEMIA
                     LLKKRGMERLWLEVRVSNDAAYNLYKKIGFTEIFRRKEYYHNPIEDAYIMELALTSEK
                     QEEAEG"
     gene            1195..2217
                     /gene="tsaD"
                     /locus_tag="PLT73_07145"
     CDS             1195..2217
                     /gene="tsaD"
                     /locus_tag="PLT73_07145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRM20190.1"
                     /translation="MTQTDVTIIAIESSCDETSVAVVKNGNAVLSNVVASQIKSHMRF
                     GGVVPEVASRHHVEQITQIVEAALNEAQVTFDAIDAVAVTHGPGLVGSLLIGVSAAKA
                     LAFAHQKPLIPVNHMAGHIYANQLVTPMRYPLLSLVVSGGHTELVYMEKDGSFDIIGE
                     TRDDAAGEAYDKIGRILGLPYPGGKKMDEMAHEGKETMHFPRAMIKEDNYDFSFSGLK
                     SSVINTVHNARQKGDDINPYDLAASFQASVVEVLVHKTLRAAKEKNIKQLLLAGGVAA
                     NRGLREALSAKMALELPEVEVVIPPLSLCGDNAAMIGAAAYTEYQQKHFAGHDLNAHP
                     GLVLGE"
BASE COUNT          655 a          472 c          627 g          566 t
ORIGIN      
        1 atccgcacac ctttgtgccg tcctacttaa aaaacccgga agctgaagaa aaatgggaac
       61 aacagcatct tagtaatcgg agggaagact acgttgagag agtggattca tcgatttaaa
      121 tccatgtata aggaaatccg atttgatgtt ctggccaaaa tccattcaaa gcgcgttctc
      181 tccaatgaga aaacctatct gctgcgtaac cagcttcctg tcagattgat ggtcggcgaa
      241 aaacagcaca ttcccgatat tctttatatc gagcggatga gctacgatgg cgcaacaccc
      301 tggggaagga cagcattgga aaatgatatg ctgtacaacc cgaagtcgct ttattacgtt
      361 ttgtatgaag gccgtatgcc tatcggtttt ttgggtgcaa gattggatgg gaaagatatt
      421 catataacga atttggcggt agtgcccgaa tttcagcaga ttggcgcggc aacgttgttg
      481 ttgcgcgtgt tgagaagctt tgcggaagaa aaaagcgcca caagcatcag cttggaagtg
      541 cggatttcaa accatcgggc gaaggcttta tacacgaaaa tgggttttct gccggagcag
      601 atcaagcagc attattatca cggtgacggc gaagacgctt tggatatggt atggcctttg
      661 gcaggaaaac aatcggaaga aaaggaagat gttcatgcaa cgcacgtttg aaatatgcga
      721 cctgaagacg gacgcaggca aattggacca acatgcacag actttgtacg aacttacaat
      781 gctggccaat gaggggcatt ccagttggaa agtatcctca ttcctgtctg aattgaagga
      841 caatcattcc atttatgtag gttatatgga ggaaaacaag ctggtgggct atatttgttg
      901 catggcggtt gtagatgagg catcaatcaa caattttgcg gtagcccctg atcatcaggc
      961 gaaaggaatc ggaacaaaat tgttgcagga aatgattgcc ttgctgaaaa aaaggggcat
     1021 ggagcggctt tggctcgaag tccgtgtttc taatgatgct gcctataacc tgtataaaaa
     1081 aataggcttt acggaaatat tccgaagaaa agagtattat cataatccga tcgaggatgc
     1141 ctacatcatg gaattggcgc tgacatcgga aaaacaagag gaggcggaag gctgatgacg
     1201 caaacagatg tgaccataat cgcgattgag agcagttgcg atgaaacgag tgtagctgtg
     1261 gtgaaaaacg gaaatgcagt cctttcgaat gttgtcgctt cccagatcaa aagccatatg
     1321 cgttttggcg gggtcgtccc ggaagtggcg agccgtcatc atgtagagca gatcactcaa
     1381 atcgtggaag cggcattaaa tgaagcgcag gtaactttcg atgctatcga tgcagttgct
     1441 gtgacgcatg gcccgggttt ggtaggttcg ttgttgatcg gtgtcagtgc ggcgaaagct
     1501 cttgcttttg cccatcagaa accgctcatt ccggtcaatc atatggccgg ccatatctat
     1561 gcgaaccagt tggtgactcc gatgcgctac ccattgctat cgttggttgt cagcggaggg
     1621 cacacggaat tggtgtatat ggaaaaggac ggatctttcg atatcatcgg cgaaacgcgg
     1681 gatgatgccg cgggggaagc ttacgacaaa atcggacgca ttttgggctt gccttatcct
     1741 ggtggaaaga aaatggatga aatggctcat gaaggtaagg aaacgatgca ttttccgcgc
     1801 gctatgatca aagaagacaa ttacgatttc agcttcagcg gattaaaaag ttccgtcatc
     1861 aatactgtcc acaatgcacg ccaaaaaggg gatgacatca atccctatga tttggccgcc
     1921 agtttccagg caagtgtcgt ggaggttctg gtgcataaga cgttgcgcgc ggccaaggaa
     1981 aagaacatta agcagctgct gttggccggc ggagtcgccg ccaacagagg cttgcgtgaa
     2041 gctttgtcgg caaaaatggc actggagctt ccggaagtcg aggtagtcat tccgccgttg
     2101 tcgctctgcg gggataatgc ggcgatgatc ggagctgcgg cttacaccga ataccaacaa
     2161 aaacattttg ccggacacga tctgaatgca catccgggct tggttttagg cgaataataa
     2221 aagagaacgc tggccgacta tgtcgactaa cgttctcttt ttttgacata caggaattta
     2281 taaaattttc cagagtgaaa agtgcatcac cgtaggtgtt
//
LOCUS       DAOMUJ010000287         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13199_length_2312_cov_1.634471, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000287 DAOMUJ010000000
VERSION     DAOMUJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13199_length_2312_cov_1.634471"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..628)
                     /locus_tag="PLT73_07150"
     CDS             complement(59..628)
                     /locus_tag="PLT73_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20191.1"
                     /translation="MIWSKMKPQLENFLAPVLVGRVEYLSTSYRYSPEKNGQCRIAVD
                     NKKIFNMKDGTTGIRWYLSEQDIKNDPAVMIPLSEAEIEQIRKETGGKVPEERLAVIA
                     SDRKLLAYAKGTMAAQVTLSKTDFNKTADVFLSQPIEDSLASKDILLNCLALVDRRVG
                     KKRIMDMEQSVRMKHPIVQYFYALRRGLK"
     gene            complement(926..1282)
                     /gene="rbfA"
                     /locus_tag="PLT73_07155"
     CDS             complement(926..1282)
                     /gene="rbfA"
                     /locus_tag="PLT73_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRM20192.1"
                     /translation="MANFRAGRIKQEIQREVNDILAKRVKDPRVANVNITDVEITGDL
                     QQATIYYSTLSELASDREKTQQGLDKATGLIRKELGTRLTLYKTPELIFKRDESVAYG
                     SKIDELIRKMHEDEKK"
     gene            complement(1325..2312)
                     /gene="infB"
                     /locus_tag="PLT73_07160"
                     /pseudo
     CDS             complement(1325..2312)
                     /gene="infB"
                     /locus_tag="PLT73_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053092.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
BASE COUNT          514 a          584 c          496 g          718 t
ORIGIN      
        1 tctggaagcc ggtttccccg ctggcgttcc ttgtggatac cgcaagcccc aatacaccct
       61 acttcaggcc acgcctcagt gcatagaaat attgcacaat cggatgcttc attctcactg
      121 attgttccat gtccatgatt cgtttcttgc cgacgcgcct atccactaag gccaagcaat
      181 tcagcagaat gtctttgctt gcgagactgt cttcgatggg ttggctcaaa aatacatcag
      241 cggttttgtt gaaatcggtt ttgcttaagg tcacttgggc ggccatcgtt cccttcgcat
      301 aggccaacag tttgcggtcg ctcgcgatca ctgccaaccg ttcttcaggt acttttccgc
      361 cggtttcttt cctgatttgt tcaatttcgg cttcgctgag cggaatcatg actgcaggat
      421 cattcttgat gtcctgttcc gacaggtacc accttattcc tgtcgttccg tccttcatgt
      481 tgaagatttt tttattgtct acggctatgc gacactggcc gtttttttcg ggtgaatagc
      541 ggtagctggt ggataggtac tccactctgc ccactaacac aggcgcgagg aagttctcca
      601 actgcggctt cattttactc caaatcataa tatcctccat cagcagcttc agtgctgtcc
      661 atttagtcta ctttacatta tgtccaaaaa tgtcatggtc ggtcaatctt tttttacaag
      721 cgaacagggc tgaatgctca cagcactaaa atcaacctct aaacgattat aactagctgg
      781 cagactctga gtcattctat ttacacaact gacctctggg tagttaaata tgggatttat
      841 gccatgttct tgaccacaaa gcaaaaaggc agccacaacg atcgcacgga tcattgtggc
      901 tgcctttttt gcacaaatat gaaatctatt ttttttcgtc ctcgtgcatc ttgcggatca
      961 attcatcaat cttgcttccg taagccactg attcatctcg tttaaagatc aattccggtg
     1021 tcttgtagag ggtcagacgc gttccaagtt ccttgcggat gaggccagtc gccttatcca
     1081 aaccttgttg cgttttttcg cgatcgctgg caagttccga caaagtgctg taatagatgg
     1141 ttgcttgctg caagtctccg gtgatttcca catcggtgat gttcacattt gcaacacgtg
     1201 gatcttttac acgcttagcc aggatatcat tgacttcccg ttgtatttct tgtttgattc
     1261 ttcctgctct gaaattagcc atttcggttc acctctcact agttagttcg attgctgtag
     1321 agcattattt cttgatttca accatatggt acgcttcgat gacatcatcg atgcggatat
     1381 cgttgtaatc ttcgatcatc acaccacatt cgaagccttt tttaacttct ttggcatcat
     1441 ccttgaatcg cttcaagctg gcaagtttgc cgtcaaaaat gacgatgttg tcacggatga
     1501 tacggatgct gctgtttctg ccgatgtagc catcggttac gaaagctcca ccgattgtgc
     1561 cgactttaga aactttatag gtctcacgga taagtgcttg accggttact tgctcttcga
     1621 attcaggatc cagcatccct ttcatcgctg tttcgatttc atcgatggca ttgtagataa
     1681 tacggtgcag acggatatcc acattttcgc tttctgcttg gatcttcgct tgaggagtcg
     1741 gacgaacgtt gaatccgatc atgattgcgt tactggctgc tgccaaagta acatcactct
     1801 cgttgatcgc tccagctgcg gagtgaacga ttttgacgcg gacaccctca acctcgattt
     1861 tttgcaagct tgctgccaag gcttcaactg aaccctgtac gtcagctttg atgatgacat
     1921 tgacctcctt caattctccg tctttcaagc tggagaacaa gttatccaag gtcacgcggt
     1981 tttgtgttga acgttgttcc ataagggcac gtttcgcacg ttcttcacct gcttgacgcg
     2041 cagtcttctc gtcttcaaag acgacaaagt gatcaccggc ttgcggtgct gcgttcaaac
     2101 ctgtctgcct tgatcgttgg tcattacgcg gacacgacca tgcgtattac cgactacgat
     2161 cggatcgccg atcttcaaag tacctgactg caccaagacg gtagcgatcg ggcctttgga
     2221 tttatccaaa cgggcttcga tgacggaacc gattgctaaa cgattcggat tagctttcaa
     2281 ttcctggact tcagcgacca acagaatcat tt
//
LOCUS       DAOMUJ010000288         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13201_length_2312_cov_1.590607, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000288 DAOMUJ010000000
VERSION     DAOMUJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13201_length_2312_cov_1.590607"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..364)
                     /locus_tag="PLT73_07165"
     CDS             complement(14..364)
                     /locus_tag="PLT73_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRM20193.1"
                     /translation="MSKNLNIWIEGTIIAALATVLSLIPFNIGPSFSVTVGAPVMLLY
                     CLRRGLVPGFFASFLWGVLHILIGTAYILTPLQGFIEYFIAFGFTGLAGLFTPKVQQA
                     ISNQNQKMHVMLIQ"
     regulatory      complement(424..525)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(640..960)
                     /locus_tag="PLT73_07170"
     CDS             complement(640..960)
                     /locus_tag="PLT73_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20194.1"
                     /translation="MAKKNKGNQIQRRNVKPTIPELHKNNTGAVTNKKLPVDEDPKLQ
                     NSFNFTMYGMLIGASIGYFAGNLTYGFGIGMLTGGIIDSYMNTQKKKKREAILKAQAE
                     KEQK"
     gene            complement(1108..1893)
                     /locus_tag="PLT73_07175"
     CDS             complement(1108..1893)
                     /locus_tag="PLT73_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM20195.1"
                     /translation="MTTIMITGASGNIGRILADHLKESYELTLVDIHFHNVDPALLEG
                     TIVKELDLTIAGNWDGLLEGIDYVIHLAGNPSPDAIFDDELIDLNYKMPYYLFKESTK
                     YENFVKRIIFASSIHAVSAYPKNVQVHVDEPVRPGDIYGVSKVYLEGLASHFAFTEGQ
                     ESIGIRIGNFDENLDDPIVDEAGLAEYLSPRDLCHLVDCALKAKLVEPFLLVNGLSDN
                     RFPRLDISQAKTALGYRPSDDAFVLKGYFQDDNGDPVELSEED"
BASE COUNT          597 a          528 c          552 g          635 t
ORIGIN      
        1 aacagactgg tacttattgt attaacatta catgcatttt ttgattttga tttgagattg
       61 cctgttgaac tttcggcgtg aaaaggcctg ccaagccagt gaatccgaag gcgatgaagt
      121 actcgatgaa tccttgcagc ggagtcagga tataggccgt cccgataagg atgtgcagca
      181 cgccccacag gaaactggcg aagaaaccgg ggaccaaacc gcggcgcagg cagtaaagca
      241 gcatgaccgg tgctcccacc gtgacggaaa aacttgggcc aatattgaaa gggatcaggg
      301 ataaaacggt tgctaaagca gcaatgatgg ttccttcgat ccagatgttc agattttttg
      361 acataaaaaa actcctcctt tttttgggca taatatacca tcgacaaagg aggagaataa
      421 aattcaattc tcaatcacaa tccctacgct cgtactaacg aacaggttcg aagggtcaga
      481 atctatgttc aatctcagcc gcggacggcc cccctttgtg aaacggttta ttcagttaag
      541 gtacacttta ctacagttga tttttttatg caagctgcac ggtgcacaaa gccgcagcca
      601 tgactatgcc ataatggcta aatacttgtg cgaaatgacc tatttctgct ctttttcggc
      661 ttgcgccttc aggatggctt cgcgcttctt tttcttctgc gtattcatgt aggaatcgat
      721 tatgccaccg gtcagcatgc cgataccgaa gccataagtc aggttgcccg cgaaataacc
      781 gattgaagcc ccaataagca tgccgtacat cgtaaaattg aatgaattct gcaatttcgg
      841 atcttcgtcg accggcagtt ttttattggt taccgcgcca gtattgtttt tatgcagttc
      901 cggtattgtt ggtttcacat ttctgcgttg gatctggttg cctttgttct ttttcgccat
      961 tcagtgtact tccttccgtc tatctgacac ttgtattgtc tccaatgaac atcatatcag
     1021 ttcatttccg gctcttcaat agaactttgc tcgcggatat acgcagaatc gtaaaaatag
     1081 tagaagtctt cccaaatcgg aggatcctta atcttcttcg gacagttcga ccgggtcgcc
     1141 gttgtcatcc tggaaatagc ccttcagcac aaaagcatca tcgctcggcc gataaccgag
     1201 ggcagttttg gcctgtgaga tgtccaagcg tgggaagcgg ttatccgata gcccgttcac
     1261 caacaggaac ggctcaacaa gtttagcttt gagggcgcag tcgactaaat ggcaaaggtc
     1321 ccgtggagac aggtattcgg ccaagcccgc ttcgtcgacg atcggatcat cgaggttttc
     1381 atcaaagttg ccgatgcgga tgccgatgga ttcttggcct tccgtaaagg cgaagtggct
     1441 ggccaatcct tccagataga ctttcgaaac gccgtaaata tcgcccggac ggacaggttc
     1501 atcgacgtga acctgcacat ttttcgggta agcgctgacg gcatggatgg aactggcgaa
     1561 gatgatacgt ttgacgaagt tttcgtactt ggtggattcc ttgaacagat agtagggcat
     1621 cttgtagttc aaatcgatca gctcatcgtc aaaaatagca tcgggtgaag gattgccggc
     1681 cagatggata acgtaatcga tcccttccaa cagcccgtcc cagttgccgg cgatagtcag
     1741 gtccaattcc ttgacgatgg tgccctccaa aagggccgga tcgacgttat ggaaatggat
     1801 atccaccaaa gtcagttcgt agctctcctt cagatgatcc gccaaaattc ttcctatatt
     1861 accgcttgcg ccggtgatca tgattgtggt cattgtcgaa ataccccctt gtttttttta
     1921 cagttgctat cgtgaacgtc tttgttttaa ctgtatgcaa gccaccggaa aatgtcaaag
     1981 gatagccctt acaaaaacgg cgattcgtcg gacgaaaagg gaatgttata tttgagtaaa
     2041 atgtaggtaa attaattgag acacgggcgt tatccctgta taataggggt aagatgatag
     2101 gcaagggcaa gacacttttc aatcgaaaac gaaggggtga gtcggatgga cttgttgaat
     2161 ttgcaaatgc gcgcttttca aggtcagtgc ggatctattg tgcaacaatc ttcccattca
     2221 ttctattgcc cgacagagca cgtgctttcg catgtgtttt tttgttgatc ctgaaaagta
     2281 ccagcggggc ttatcatcat aatgccgagc at
//
LOCUS       DAOMUJ010000289         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13286_length_2300_cov_1.498886, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000289 DAOMUJ010000000
VERSION     DAOMUJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13286_length_2300_cov_1.498886"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..862
                     /locus_tag="PLT73_07180"
     CDS             131..862
                     /locus_tag="PLT73_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRM20196.1"
                     /translation="MKAYIIKIELLDSYPLIWRRVVMPADATFRRLHDVIQTVTNFLS
                     GGSYGGYHLYEFDLSAENITVTNDEESFEEYQYYKQNKKQYEERLRLAPIGSFQRSSL
                     ERLMKTVVRQPSRIKIDKYLEKYKEIRYSYDFGDGWEFLITLEQTVDDYHFGYPTLLD
                     GAESAPPEDVGGLGGFEEFLKIYRDPSHPEYAEMKEWADSQEFREYDPDWINNSLKYL
                     NYKKTEWDQINHDNYFIIEDKYRKN"
     gene            918..1061
                     /locus_tag="PLT73_07185"
     CDS             918..1061
                     /locus_tag="PLT73_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20197.1"
                     /translation="MKSDPVEGKPCAGYIVLRRANAGRRTMRGGWPNSGEAAVVGGND
                     YFV"
     gene            1404..2150
                     /locus_tag="PLT73_07190"
     CDS             1404..2150
                     /locus_tag="PLT73_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRM20198.1"
                     /translation="MNQLTEYIIALSNLYGMIQKDILVEIYNTQNEEPISLGEVEEYL
                     ENPPPELKKGHTYPYKDYFVHETILEFDEFESMLEKKGDKPFYVPAKEELLRYTDDFY
                     FEKTKQYKVFYDYVRKNLLGGDGVKAQELCEEIRDALELDLGMSSVSKALERADVVFD
                     NEQQVNEMMRLMMDMSNHIRRWEHNGNTPQEIFEEFEKPHLRPLPKKSYRVGASNGGP
                     FEKKVKIGRNDPCPCGSGKKYKNCCINKVE"
BASE COUNT          636 a          486 c          636 g          542 t
ORIGIN      
        1 tggaaatcaa ccagatctat aacaaattgt cgcggtacgc tctggccgat gccgccacga
       61 agcaagcgca catcgatgcg atgaaatggc gcaattagcg actgtattag agaaagcgag
      121 gaacttaatt atgaaagcat atatcataaa aatcgaactt ctggattcat atcccttgat
      181 ctggcgcagg gtggtcatgc ccgccgatgc gacgttcagg cgcctgcacg acgtcatcca
      241 aaccgtgacc aatttcctga gcggaggctc ttatggcggc taccatctct acgaatttga
      301 tctgtcggca gaaaatatca ctgtcacgaa tgacgaagaa agctttgagg agtatcaata
      361 ttacaagcaa aataaaaagc aatatgaaga acgactaaga ctggcgccaa ttggatcatt
      421 tcagcggtcc agtctggagc gcttgatgaa aactgtggtg cgccaaccat cccggattaa
      481 aatcgacaag tatctagaaa aatacaaaga gattcgctat tcgtacgatt ttggcgacgg
      541 atgggagttt ctgattactc ttgaacagac tgttgatgat tatcactttg gctacccgac
      601 tttgcttgat ggtgccgaat cggctccgcc tgaagatgta ggtggcttgg gtggtttcga
      661 ggagttcctg aaaatctacc gtgatccatc gcaccccgaa tatgcggaaa tgaaagaatg
      721 ggccgactca caagagttca gggagtacga cccagactgg atcaacaact ccctgaaata
      781 cctgaactac aagaagaccg agtgggacca gatcaaccac gataactatt tcatcatcga
      841 agacaaatac cggaagaatt gaaccctgaa aagaccagtt gcggccgcac tggtcttttt
      901 gcgcggacct acctccgatg aagagtgacc ctgtcgaagg taagccttgc gccggctaca
      961 tcgtcctccg gcgagcgaat gctggccgga ggacgatgcg tggtgggtgg ccgaactccg
     1021 gcgaagccgc tgtggtcgga ggtaatgatt attttgtgtg atttcctccg gcagaagggt
     1081 gccttcccgg aggtgggcgt cagctccgat gagtggggtg ctcatcggaa gacagactgc
     1141 gccgtggagg ccaactccgg cgagcgaatg ctggccggag caggctgcat tgtgggtatc
     1201 tgtactccgg ccgaacttcg gcgaagccgc ggtggtcgga ggtaatgatt attttgtgtg
     1261 atttcctccg gcagggaagc ccttcgcagg aggaaaataa ataaagccca ctatttttca
     1321 ggttattctg tattccttct gctcttctat agtaaaatag acaaaagaag gaatgcaaaa
     1381 taataaccga gcggagtgac agcatgaatc aattgacgga atatatcatc gctttgagca
     1441 atttgtacgg catgatccag aaggatattt tagtggaaat ttacaatacc caaaacgagg
     1501 agccaatcag cttgggggaa gttgaggagt acctcgagaa tccgcctccg gaactgaaaa
     1561 aggggcatac ttatccatac aaggattatt tcgttcacga aacgattttg gagttcgatg
     1621 agtttgaatc gatgctggag aaaaaagggg acaagccttt ttatgttccg gcgaaagagg
     1681 agcttttgcg ctacacggac gatttttatt ttgagaagac gaagcaatat aaggtttttt
     1741 atgattatgt gagaaaaaat ttgcttggtg gtgatggggt caaggcccag gagctttgcg
     1801 aggaaatccg tgatgcgtta gagttggatt tgggcatgtc atctgtgtct aaggcattgg
     1861 agcgtgcgga tgtcgtgttc gataatgaac agcaggtcaa tgaaatgatg cgcctgatga
     1921 tggacatgtc gaatcatatt cgccgttggg aacataatgg caatacgccc caggagattt
     1981 tcgaggagtt cgaaaagccg cacttgcgtc cattgccgaa aaaatcttac cgcgtgggcg
     2041 cttcgaatgg gggccctttt gagaaaaaag tgaagatcgg ccgcaatgat ccgtgtccat
     2101 gcggcagcgg gaagaagtat aagaattgtt gcataaataa ggtggaataa aaaagaagaa
     2161 tcatttgatc atatagcccg cgcaactccg acaagagcat ccttgccgga gttgcgcggg
     2221 cttgtttgcg ttgtactccg gcgagagaat gttggccgga ggaggatgcg ttgcgggagg
     2281 ctgaactccg gcgaagccgc
//
LOCUS       DAOMUJ010000290         2299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13295_length_2299_cov_1.666221, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000290 DAOMUJ010000000
VERSION     DAOMUJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13295_length_2299_cov_1.666221"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>677
                     /locus_tag="PLT73_07195"
     CDS             <1..>677
                     /locus_tag="PLT73_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20199.1"
                     /translation="SIPFVIFIFFQLTGILPLSTSGIVKGGFILTAVWWFIFTIPYWK
                     NVKQNYYIEKEPHVVRKSFVRLWETLKNIKEYRQAFLFLLAYFFYIDGVGTIFKMATA
                     VGSDIGLSSNELIVVMMVVQFVAFPFSILYGRASKRFGNKNMIFVGIVTYIFICIYAL
                     QLDSLLTFIILALLVGTAQGGVQALSRSLFGQLIPKEQANEFFGFYNIFGKFAAVVGP
                     LLVGLIS"
     assembly_gap    678..739
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            935..>2299
                     /gene="dnaG"
                     /locus_tag="PLT73_07200"
     CDS             935..>2299
                     /gene="dnaG"
                     /locus_tag="PLT73_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRM20200.1"
                     /translation="MAERISEEKLAQIRTETNIVDVVSQYVQLKKRGKNHFGFCPFHD
                     EKTPSFSVAEEKQIFHCFSCGRGGNVFTFLMDVEGISFVEAVIKTAELSNIALDFSYE
                     SHAQDNPLQSKKEKLIQIHEEAAAFYHQVLMNTVTGQAALDYMIQRGFTPETLKEYQI
                     GFSPSNRTALFQMLKAKAFDEGLLQESGIFTDRQGQNELYDRFSSRIIFPLRNAKGKT
                     VAFSGRILHASPVDDTGYHEAKYLNSPETLLFNKRDFLFNFDKARSEIRRHSEVILFE
                     GYMDVISAWQAGVKNGVASMGTSLTEEQNRILTKTADKIVIAYDGDRPGVEATKRAIE
                     ILERNKHFDISIFPLEAGMDPDEYIQQKGPEAFAKALSNNRETVIQFYSRYLKMNLNL
                     DSEKNRITYIETMLKALAPLDSLVERELYMKDIADEFSIPIDILQKQLKGYQQEVLQQ
                     RPERE"
BASE COUNT          606 a          494 c          537 g          600 t
ORIGIN      
        1 ttccatccct ttcgtcatct tcattttttt ccaattgacc ggcattttgc cattgagcac
       61 gagcggcatt gtgaagggcg gcttcatcct gactgcggtt tggtggttca tcttcaccat
      121 cccttattgg aagaatgtca agcagaacta ttacatcgaa aaagagccgc atgtggtccg
      181 caagagtttt gtcagattat gggaaacgct gaagaacatc aaggaataca gacaagcctt
      241 tttatttttg ttggcttatt tcttctacat cgacggagtt gggaccatct tcaagatggc
      301 gactgcggtt ggatcggata tcgggctctc ttcgaatgaa ctgatcgttg tcatgatggt
      361 ggtccagttc gttgctttcc cgttttccat cctgtacggc cgagcatcca agcgcttcgg
      421 caacaagaac atgatttttg tgggcattgt aacctatatt tttatctgca tttatgcgct
      481 gcagttggac tcgttgctga cattcatcat tttggcgctg cttgtgggca cagcccaagg
      541 cggcgtgcag gcgttgagcc gatccttatt cggtcaattg atcccgaaag agcaagcgaa
      601 cgaatttttc ggattctata acatcttcgg gaaatttgcg gctgtggtcg gtccgctgct
      661 ggtcggactc atctcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnt tttgccctga tcatcctgtt cgtgatcggc gggatactgc
      781 tgttctttgt acaagttccg gatccgcaag caaagcagca gagctgacca attggattaa
      841 agtttgcttg tgtgccaaaa atgtggtaaa atctaatttg catctttatg ctcgttttta
      901 taaaatggag agatttcttc aaggtgggag acatttggca gaacgtattt cagaagaaaa
      961 attagcgcaa atcagaaccg aaacgaatat tgtcgatgtc gtcagccaat atgtacaact
     1021 taaaaaaaga ggcaagaacc atttcggatt ttgcccgttc catgacgaaa agaccccctc
     1081 tttttctgtg gcagaagaaa aacagatttt tcactgcttc agttgcggaa gaggtgggaa
     1141 tgtttttact tttttgatgg atgtggaggg tatttccttt gttgaagcag tcattaagac
     1201 agccgaatta agcaacattg ccttggattt ctcgtacgaa agccatgcac aggataatcc
     1261 gctgcagtca aaaaaggaaa aactgatcca aatccacgag gaagcagctg ccttttacca
     1321 tcaggtcctg atgaataccg tcacggggca agctgcgctt gattacatga tccaacgcgg
     1381 cttcacgccg gagacactga aagaatatca gataggtttt tctccatcca atcggacggc
     1441 actctttcaa atgctgaaag cgaaggcgtt cgatgaaggg ctccttcagg aaagtggcat
     1501 attcacggat agacaaggcc aaaacgaact ctacgatcgg ttctcttcca ggatcatttt
     1561 cccattgcgt aatgctaaag gaaagacggt cgctttttcc ggcagaattc tgcatgcttc
     1621 cccagtggat gacacaggtt atcatgaagc aaaatacttg aacagccctg aaacactttt
     1681 gttcaacaaa cgggatttcc ttttcaactt cgataaagcc cgcagtgaga tccgaaggca
     1741 ttcagaagtg atccttttcg aaggatacat ggatgtcata tccgcttggc aagcgggagt
     1801 gaaaaatggt gttgcctcga tggggaccag tctgacggaa gaacagaacc ggatattgac
     1861 gaagaccgcc gataaaatag tcattgccta tgacggtgat cggcctggtg tcgaagcgac
     1921 caagcgcgcc atagagatac tggaacgcaa caagcacttc gatatctcca ttttcccgct
     1981 ggaagcaggc atggacccgg atgaatacat ccagcagaaa ggtccggagg catttgctaa
     2041 agcactgagc aacaacaggg agacagtcat tcagttctat tcacgctacc tcaagatgaa
     2101 tctgaacctg gattctgaaa aaaatcgcat cacctatatc gaaactatgc ttaaggcatt
     2161 ggctcccttg gattctctgg tcgaaagaga gctttacatg aaggacatcg cggatgaatt
     2221 cagtattccg atcgatattc tgcagaagca attgaagggt tatcagcaag aggtgcttca
     2281 gcaaagacct gaacgcgag
//
LOCUS       DAOMUJ010000291         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13310_length_2297_cov_2.449599, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000291 DAOMUJ010000000
VERSION     DAOMUJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13310_length_2297_cov_2.449599"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLT73_07205"
     CDS             complement(<1..651)
                     /locus_tag="PLT73_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20201.1"
                     /translation="MASISIREWGSIGTEQVMRIDLSQPNGMSVSCINYGARLIRLLV
                     PDREGQSENVVLGLADAEAYGNDQASFGATVGPVAGRIAEGKWGAVQLEQNNSAHHIH
                     GGSRGWGQQFWGFTTEETAEAVSVTFSLESTPETVGYPGRLQAKVGYSLDAEGGLTIT
                     MEGISRGTEETLFNPTSHIYFNLSGDAKRPITDHVLQLACEEVLELDADKLPTGTKQ"
     gene            complement(644..1318)
                     /gene="pgmB"
                     /locus_tag="PLT73_07210"
     CDS             complement(644..1318)
                     /gene="pgmB"
                     /locus_tag="PLT73_07210"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRM20202.1"
                     /translation="MLKAVLFDLDGVITDTAKFHFLAWRDLGAELGVTVDEAFNEELK
                     GVSRIDSLERILAFGGLADKYSTAEKEALCTKKNDHYLELIQEMTPADILPGILPLLE
                     ELREAGLKAIVTSASKNAPGILELLQVKDYFDGIVDPASVAAGKPAPDIFLAGAELAG
                     AKPAECIGVEDAASGVEAIKAAHIPAIAIGDAEVLAQADRVLADTSDLSLAVLRDTWE
                     KATANG"
     gene            complement(1342..>2297)
                     /locus_tag="PLT73_07215"
     CDS             complement(1342..>2297)
                     /locus_tag="PLT73_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRM20203.1"
                     /translation="ESCLAEEGLEVLSAVSRFWADRVHFSQNKQCYMIHGVTGPNEYE
                     NNINNNWYTNRLAVWVLRYTLESLEKYPERSAALGITAAEKAKWQDIIDNMYFPYDEE
                     RQVFVQHDTFLDKDLKPVSALDKADLPLNQHWSWDKILRSCFIKQADVLQGIYLFGDE
                     FTLEEKERNFDFYEPMTVHESSLSPCIHAILAAELHKEEKAVEQYQRTARLDLDNYNN
                     DTEDGLHITSMTGSWLTIAEGFAGMRAKDGLSFAPFLPKDWTHYQFHINYRGSLILIA
                     VNEEHVSLTLVKGEALSVRIYDEELQLKDEIKVALKKTVSA"
BASE COUNT          441 a          655 c          612 g          589 t
ORIGIN      
        1 ttgctttgtc ccggtcggaa gcttatcggc atccaattcc agcacttctt cgcaggccag
       61 ctgcaaaaca tggtcggtta tcggacgttt ggcatcgcca cttaaattga aatagatatg
      121 gctggtcgga ttgaacagcg tttcttcagt gccccggctg atcccttcca tcgtgatcgt
      181 caggccgcct tccgcatcga gggaatagcc gacttttgcc tgcagacggc cgggatagcc
      241 gactgtttcc ggtgtcgatt ccaacgagaa agtgacgctc accgcttccg ccgtttcttc
      301 ggtcgtaaat ccccagaact gttggcccca accgcggctt ccgccgtgga tatggtgtgc
      361 gctgttattc tgttccagct gcactgcgcc ccatttgcct tcagcgatcc ggccggcaac
      421 tggtccaacg gtcgcgccga aggatgcctg gtcgttaccg taggcttcgg catcagccaa
      481 gcccaggaca acattttcgc tctggccttc ccggtccggg acaagcaagc ggatcagccg
      541 tgcgccgtag ttgatgcagc tgacggacat gccgttcggc tggctcaaat cgatgcgcat
      601 gacttgttcc gttccgatgc tgccccattc gcggatagag atgctagcca ttggccgtcg
      661 ccttctccca cgtatcgcgc aggacggcaa gcgacaagtc gctcgtgtcc gccaagacgc
      721 ggtcggcttg cgccagcact tcagcgtcgc cgattgcgat cgcaggaata tgcgcagctt
      781 tgatggcctc cacgcctgaa gcggcatctt ccacgccgat gcactcggct ggttttgccc
      841 ctgccagctc cgcgccggcc aagaagatgt ccggagccgg tttacctgca gccactgagg
      901 ctggatcgac gatgccgtcg aagtagtcct tgacctgcaa aagctccagg atgccaggtg
      961 cgttcttgct ggcggaagtc acgatcgcct tcagacccgc ttcgcgcaat tcttccaaaa
     1021 gcggcaggat gccaggcagg atgtccgccg gtgtcatttc ctggatcaat tccagataat
     1081 ggtcattttt ctttgtgcac aaggcttctt tttcagctgt ggaatattta tccgccaagc
     1141 cgccgaatgc caggatgcgc tcgagcgaat cgatgcggct gacacccttc agctcttcat
     1201 tgaaggcttc gtcaacggtg acccccaact ccgcgccgag gtcacgccag gccaaaaaat
     1261 ggaatttggc agtgtcggtg atgacgccgt ccaagtcgaa caatactgct ttcaacattt
     1321 ctttctctcc tttggtgccg ctcatgcgga cacggtcttt ttcagtgcga ctttgatttc
     1381 atctttcagt tgcagttctt catcatagat gcggacagac aacgcttcgc cttttactaa
     1441 ggtcaggctg acgtgttctt cgttcaccgc gatcaggatc aagctaccgc ggtagttgat
     1501 gtggaattgg tagtgcgtcc agtctttcgg caagaacggc gcgaagctca gaccgtcttt
     1561 ggcgcgcatg ccggcgaagc cttccgcaat cgtcaaccag ctaccggtca tcgaagtgat
     1621 gtgcaagccg tcttcggtat cattgttgta gttgtccaga tccaggcggg cagtgcgttg
     1681 gtactgttcc acagcttttt cttccttgtg cagctcggcc gcaaggatcg cgtggatgca
     1741 tggcgacaag cttgattcat gcacggtcat cggctcgtag aagtcaaagt tacgttcttt
     1801 ttcttccagc gtgaattcgt caccgaacag gtaaatccct tgcaggacgt cggcttgttt
     1861 gatgaagcaa gaacgcaaaa tcttgtccca tgaccagtgt tggttcaatg gcaaatcagc
     1921 tttgtccagc gcgctgaccg gtttcagatc tttgtccagg aacgtgtcgt gctgcacgaa
     1981 aacttggcgc tcctcatcgt acgggaagta catgttgtcg atgatgtcct gccattttgc
     2041 tttttcggct gcggtgatgc ccaatgctgc tgagcgttcc ggatacttct ccaagctttc
     2101 caaagtataa cgcagcaccc agactgccaa acggtttgtg taccagttgt tgttgatgtt
     2161 gttttcgtac tcgttcggtc cggttacgcc atggatcatg tagcattgct tgttttgtga
     2221 gaagtgtacg cggtccgccc agaaacggct gacagcgctg agcacttcca gcccttcttc
     2281 ggccaggcag ctttcat
//
LOCUS       DAOMUJ010000292         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13311_length_2297_cov_1.908564, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000292 DAOMUJ010000000
VERSION     DAOMUJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13311_length_2297_cov_1.908564"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>349)
                     /locus_tag="PLT73_07220"
     CDS             complement(81..>349)
                     /locus_tag="PLT73_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM20204.1"
                     /translation="AEQLQHDKDSLFDELDRYARANGVAVHKEMRSGNAKEMIAKALV
                     EEWNCDLIVMGATGKGTIAKLVVGSVTNHVIKHAPCDVLIVRKN"
     assembly_gap    350..401
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(704..>1480)
                     /locus_tag="PLT73_07225"
     CDS             complement(704..>1480)
                     /locus_tag="PLT73_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRM20205.1"
                     /translation="GDDAEWHTIEESMRPLMKTSEVEMIPGFSTGTFMEVIDESGKMI
                     IGLAEMDIYEYMQPKWLLKHLATLKRAKTIIIDSNCPKESVEHLLEIGAKYNIPTVLI
                     CASVLKLYNIPENLKGLKLLITKHDETEKHFGIKIKDDASMREALQMWLDKGVQHVII
                     TKNSQSVGYASENYGMHVYDLKNSDENSETYIWGTNEALCAGIVYSYLRTDEISEMIQ
                     TGLANAVMSSKSAYKVRPNLSQAVLRKDVKEIGKLESREI"
     assembly_gap    1481..1518
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1519..1816)
                     /locus_tag="PLT73_07230"
     CDS             complement(<1519..1816)
                     /locus_tag="PLT73_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRM20206.1"
                     /translation="MALNEKESKILSYIKENPFISQQDLATKIGLSRPAVANIISGLV
                     RRGYLLGKAYVINDTRPIVCIGAACIDRRYFVEGGLIHGQSNNVTSQTSIGGVAL"
     gene            2041..>2297
                     /locus_tag="PLT73_07235"
     CDS             2041..>2297
                     /locus_tag="PLT73_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRM20207.1"
                     /translation="MIHTFIFDVDGTIVDTEDSIFRGLDDILQEHAGRPATQEDMRTI
                     FGIPGMEGLQHLGFTPEQAADLHPKWSAQSKTYADSVSIFAG"
BASE COUNT          566 a          531 c          438 g          672 t
ORIGIN      
        1 cgatataaag gaaagtccag ctacatttaa gtaactggac ttttttgcgt ctcctgattc
       61 gttggttcaa aagcgtctga tcaatttttc cttacgatta atacatcaca tggcgcatgt
      121 ttgatgacat gattcgtcac cgatccgacc accagttttg cgatagtgcc cttacctgta
      181 gcacccatta caatcaaatc acaattccat tcttccacaa gcgccttcgc aatcatttcc
      241 tttgcgttgc cagagcgcat ttccttatgc actgcgactc catttgctcg cgcatacctg
      301 tccaattcat cgaataaaga atccttatca tgctgcagct gttcagcttn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttcagcga tatgtaccgc
      421 ctgcttgaac gccttgattg aagcctccga tccgtccact ggaatcaaga tgtttttata
      481 ctgaaccatt caaacccact cctttggaat aatcaaccag aaaacgcttt ccttactctt
      541 attttactat acttacgaaa taaacaaaag attagtcgtc ctacggttga taaatttaca
      601 aaccaaaaag agcctggttg acaggctctt tttggtaact gcgctactca gatcagtaga
      661 gtagtggact atttattttt ttgaatgtaa cgggcttctc cagctaaatt tctctgcttt
      721 ccaatttgcc gatttctttg acatccttgc gcaacaccgc ctgggatagg ttcgggcgaa
      781 ctttgtaggc tgattttgat gacatgactg cattcgctaa gccagtctgg atcatttctg
      841 aaatctcatc agtcctcaaa taggagtaga cgattccggc gcaaagcgct tcattcgttc
      901 cccagatgta ggtttcgctg ttttcgtcgc tgttcttcag atcatagaca tgcatgccgt
      961 aattttcact ggcatagccg acgctctgac tgtttttggt gatgatgaca tgctgtaccc
     1021 ctttgtcaag ccacatctgt agggcttccc gcatagaagc atcatccttg attttgatgc
     1081 cgaagtgctt ctcggtctcg tcatgcttcg tgatcagcaa cttcaagccc ttcaagtttt
     1141 cgggaatatt atacaattta aggacggatg cgcaaatcag gacggttgga atattgtatt
     1201 tcgctccgat ttcaagtaag tgttccacgc tttctttcgg gcaattcgaa tcgatgatga
     1261 tagttttggc tcgtttcagg gtcgccaggt gcttcaacaa ccatttcggc tgcatatact
     1321 catagatatc catttccgcc aatccgatga tcattttacc cgattcatcg atgacttcca
     1381 taaaggtgcc tgtcgaaaag ccaggaatca tttccacttc ggaggtcttc atcaatgggc
     1441 gcatagattc ttcgatcgta tgccattccg catcatctcc nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnntg agagcaaccc cgccgattga cgtctgggag gtaacattgt
     1561 tcgattgtcc atgaataaga ccgccttcca caaaatagcg gcgatcaata caggccgcac
     1621 cgatacagac aatcggtcgc gtatcattga tgacataggc tttccccaaa aggtatcccc
     1681 gtctcaccaa gccggaaatg atgttcgcca ccgcgggacg tgacaggcca atctttgtag
     1741 ctaaatcttg ctgcgaaatg aaaggatttt ccttaatgta gctcagtatt tttgattctt
     1801 tctcgtttaa agccatgttt caattcccct tactctataa aaactcggca atgccgaact
     1861 tattctttta gcaacatttt tctgctccta aatgtatagt atatatccaa tgactgctgt
     1921 tgccaaacaa acacggtata ttgtaacctt ttcttaattc ctttgcatca ttcaggggga
     1981 ttttgttact aaacattact atgctataat aacaagaaat atttcaaggg agttgcacat
     2041 atgattcata catttatttt tgatgtcgac ggtacaatcg ttgatactga agattccatt
     2101 ttcagaggtc tggacgatat ccttcaggaa cacgccggca gacctgccac acaagaagac
     2161 atgcgtacaa tattcggcat accggggatg gaaggtttgc agcatttggg cttcacaccc
     2221 gaacaagctg cggatctgca tccgaagtgg agtgcgcagt cgaaaactta tgcggacagc
     2281 gtgagtatct ttgccgg
//
LOCUS       DAOMUJ010000293         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13358_length_2291_cov_2.042487, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000293 DAOMUJ010000000
VERSION     DAOMUJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13358_length_2291_cov_2.042487"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLT73_07240"
     CDS             complement(<1..188)
                     /locus_tag="PLT73_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HRM20208.1"
                     /translation="MPDKLTNLGFTDRFAQEATLNPDLHPARVIVQYKELYRVATAES
                     EMLAEISEKMRHAAVDTPG"
     gene            complement(222..1142)
                     /locus_tag="PLT73_07245"
     CDS             complement(222..1142)
                     /locus_tag="PLT73_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRM20209.1"
                     /translation="MVYISTKEASNNWDISERRIRGLCQEGRIEGAVKIGRNWAIPVD
                     AHKPVDARQSEQKKYLGLGYDFSQIDTAMDTINRHRPFSKGLADSLHEKLIVEWTYNS
                     NAIEGNTLTMSETKVVLEGITIGGKSLVEHLEIINHRDAILFIEQLVSEKEPLSEWNI
                     KNIHALVLKQIDNQNAGKYRSENVVISGAKHIPPKHYQISEYMQKLIAEYQNEWLGYH
                     PVVRATLLHGEFVKIHPFIDGNGRTSRLLLNFELMKSGYPPIIIKNENRADYYKVLDH
                     AHTTMDYGPFIKLVAELVIESEKLWLMVLE"
     gene            complement(1297..1806)
                     /locus_tag="PLT73_07250"
     CDS             complement(1297..1806)
                     /locus_tag="PLT73_07250"
                     /inference="COORDINATES: protein motif:HMM:NF037716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRM20210.1"
                     /translation="MKNPKQVDFIFAFAFCAVLLVGCTNSPPSNDAGLVAETDQMLTE
                     SSPQERDDAMPMMTIQVGNTRYAVSLNDNATTQALTQKLPMTLKMDELNGNEKFHYLL
                     ETLPTDSQRVGRIKAGDVMLYGSDCLVIFYKDFSTSYSYTPIGRIEDPSGLAQALGRG
                     NVEVTFSNR"
     gene            complement(1823..>2291)
                     /locus_tag="PLT73_07255"
     CDS             complement(1823..>2291)
                     /locus_tag="PLT73_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM20211.1"
                     /translation="ADSMTDEQRTQLFDQLGEQRWKDFQGNEPELTPTFPSEPVDSIP
                     EGMDPISSEFFGYYAMKRGFHPNSIGAFTKTSAMDFMNFPLMNYIQTISPRPILFIIG
                     EHAHSRYFSEDAYNMAAEPKELFEIAGAGHVDLYDRTDLIPFDKLESFFTENL"
BASE COUNT          534 a          572 c          501 g          684 t
ORIGIN      
        1 cccggcgtat cgactgcagc atggcgcatt ttctcggata tttcggccag catctccgat
       61 tctgcggtcg caacccgata caattccttg tattgcacga tgactcttgc cggatgcaga
      121 tccggattaa gggtcgcctc ctgcgcgaac ctgtccgtga atcccagatt tgttaatttg
      181 tcaggcattt tcttcccttc ctttcgaaca aaaccgctct gtcattccag aaccattaac
      241 cacaattttt ccgactcaat cacgagttcc gctaccaatt ttatgaaagg tccgtaatcc
      301 atggtcgtgt gggcgtgatc aagcacctta taatagtcag cacggttttc gtttttgatg
      361 atgattggtg gatagccgga tttcataagt tcaaagttca acaacaaccg tgatgtcctg
      421 ccgtttccgt caatgaacgg gtgaattttc acgaattcgc catgcagtaa agtcgcccgc
      481 acaacggggt gatacccaag ccattcgttc tgatattcgg caatcaattt ttgcatatat
      541 tcgcttattt gataatgttt cggcggaata tgctttgcac cactgatcac cacattctcg
      601 ctccgatatt tccccgcatt ttgattatca atttgcttca gaaccagcgc atgaatgttc
      661 ttgatgttcc attcggagag cggttctttt tcagaaacaa gttgttcgat gaaaagaatg
      721 gcgtcgcggt gattgatgat ttccagatgt tcgaccaggc tttttccgcc tatcgttatc
      781 ccttcgagaa cgactttcgt ttctgacatg gtcagtgtgt tcccttcgat cgcattgctg
      841 ttgtaagtcc actcaacgat cagtttttca tggagggaat ccgccaatcc ctttgaaaac
      901 ggtctgtgtc tattgatggt atccatcgcc gtatcgattt ggctgaaatc gtatcccaag
      961 ccgagatact ttttttgttc actttgcctt gcatccacag gtttgtgcgc atcaacaggg
     1021 attgcccagt tccgaccgat tttgactgcc ccttcaatcc gaccttcttg gcaaagtccc
     1081 cgaatcctgc gttcgcttat gtcccaattg ttgcttgctt cttttgtgct gatgtatacc
     1141 ataatcgctc acctcaactc cattttatgc ttcctagtgt tgaaaagcaa tatcattccg
     1201 acctcggaat gataggtaat gaacagttgg tggaattcca gctcaacaaa gtaaaaagcg
     1261 agaaatggtt caaaacccat ctctcgcttg attctgttat cggttgctga atgtgacctc
     1321 gacattccct cgtcccagcg cttgggcaag tccagatggg tcttcaatcc tcccgattgg
     1381 cgtgtagctg taggaggtcg aaaaatcttt gtagaaaatc accagacagt cggaaccgta
     1441 caacatcaca tccccggctt tgattctgcc gactcgttga gagtccgttg gcaaggtctc
     1501 caaaagataa tgaaattttt cattcccatt cagttcatcc atttttagag tcatcggcaa
     1561 cttttgggtc agtgcctgcg ttgtcgcatt gtcattcagc gacacagcat aacgggtgtt
     1621 tccgacttga atcgtcatca ttggcatggc gtcatccctt tcttgtgggc tggattcggt
     1681 gagcatttgg tctgtttctg caacaagtcc ggcgtcattc gacggcggac tattggtgca
     1741 gccgaccagc aaaaccgcac aaaatgcgaa tgcaaaaata aaatcaactt gtttaggatt
     1801 tttcatctga accacatctt ttttacagat tttccgtaaa gaacgactcc agtttatcga
     1861 acggaatcag atccgttctg tcgtacagat ccacgtggcc agcaccggca atttcaaaca
     1921 gttctttcgg ttcggccgcc atgttgtaag catcctcgct gaaatatctc gaatgcgcat
     1981 gctcaccgat gatgaacagg atcggtctag gagaaatcgt ctggatgtag ttcatcaacg
     2041 ggaagttcat gaaatccatg gcgctcgtct tcgtgaatgc gccgatcgag ttcgggtgga
     2101 atccgcgttt catcgcataa tagccgaaga attcactgct gatcggatcc attccttcag
     2161 ggatagaatc cacaggctcg cttgggaaag taggcgtcaa ttccggttca ttgccctgga
     2221 aatctttcca acgttgctca cccagttggt caaaaagctg tgtgcgttgt tcatccgtca
     2281 tgctgtctgc c
//
LOCUS       DAOMUJ010000294         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13383_length_2289_cov_2.215309, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000294 DAOMUJ010000000
VERSION     DAOMUJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13383_length_2289_cov_2.215309"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..530)
                     /locus_tag="PLT73_07260"
     CDS             complement(54..530)
                     /locus_tag="PLT73_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRM20212.1"
                     /translation="MKIAVSSDHAGFALKEEVKALLEQEGHEVLDFGPYNEEPCDLSD
                     YVYPASLAVAEGKADRGIFVDGVGYGSALIANKIYGVYAAVCQDPFCAKLARSHSDTN
                     VLCLGGKIIGSALALEIVRAWMTTDYLSDIEKYTNRVDKVKKIAEKHLKKLDEIQD"
     gene            725..>904
                     /locus_tag="PLT73_07265"
     CDS             725..>904
                     /locus_tag="PLT73_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRM20213.1"
                     /translation="MGEIKGLVFDLDGVITDTAERHYLAWKRIAHRLGIAIDREWNER
                     LKGISRKESLELILAE"
     assembly_gap    905..926
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <927..1402
                     /locus_tag="PLT73_07270"
     CDS             <927..1402
                     /locus_tag="PLT73_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM20214.1"
                     /translation="QLLKEKNAHYLELIAEITAADILPGIPELLEEAKNAGYRMAIAS
                     ASKNAPLILERLGLSAMFDAVVDPESLKQNKPHPEIFEKAAQALGLETQECIGFEDSL
                     AGIEGIKRAGMPAVAIAIPIFAEWLPDIEVAATKDLKLEEIVRKVTTMKERVEEW"
     gene            1396..>2289
                     /locus_tag="PLT73_07275"
     CDS             1396..>2289
                     /locus_tag="PLT73_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM20215.1"
                     /translation="MVDLKGNPFYLKDEQIAWVNRTLENLTLDEKVGQLFCPIGMSTD
                     EKALFDLAANKKVGGLLFRPGKGEEVQQAHRYLQHQAKVPMLIAANLEAGGTGSAIDG
                     TRIGNPMLLAAGQDAEFGHHLGEVCAKEGSAVGVNWAFAPVVDIDYNFHNPITNVRTY
                     GSDPETVLNYAKGFVEAVQQNGVAASIKHFPGDGIDFRDQHLVTTVNTLLLEEWRSTY
                     GKVYSGLIEAGSLSVMIGHIALPAVQKSEEDAFLPATLSPVIMQELLREELGFNGVIT
                     SDATPMVGFCAAMERRLAVPTA"
BASE COUNT          588 a          515 c          611 g          553 t
ORIGIN      
        1 ttatttttga agtaattttt gcttcgaaca gcatgcagtc aattcatgac tgactagtcc
       61 tggatttcat ccaatttctt cagatgcttc tccgcaatct ttttcacttt gtcgacgcgg
      121 ttcgtgtatt tttcgatatc gctgaggtaa tcggtcgtca tccaagccct cacgatttca
      181 agcgccaatg ccgatccgat gatctttccg ccgaggcaga gaacattcgt gtcggagtgg
      241 gaacgcgcca gtttcgcgca gaaaggatct tggcagacag ccgcatagac gccgtagatt
      301 ttgttggcga tcagggcact gccgtaaccg acgccatcga caaagatacc gcggtcggct
      361 tttccttctg ccacagccag tgaggcagga taaacataat cggacaggtc acaaggttct
      421 tcgttgtaag gtccgaagtc gagcacttca tggccttctt gttcgagtaa agctttgact
      481 tcttctttca atgcgaagcc ggcatggtcg ctggaaacgg caattttcat ctaaatccct
      541 ccattatcta tcattaacca aaagtataag ccaaacggcg agaggagtac agactgagtt
      601 tacagatcaa gccagaacaa acctgtgatt tgaacagtag ccactttgat tctgccattg
      661 ttcgggatgc ctcaattatg taagatgaag atgtaaacta ggattcttcg aatggagtag
      721 acaaatgggc gaaataaaag gactggtatt tgatttggac ggtgtcatca ctgatacagc
      781 tgaacggcat tacttggcat ggaaaaggat agcgcatcga ttgggcatcg cgatcgaccg
      841 cgaatggaac gaacgcctca aaggcatcag cagaaaggaa tctttggaac ttattttggc
      901 tgagnnnnnn nnnnnnnnnn nnnnnncaca gcttttgaaa gaaaaaaatg cgcattactt
      961 ggagttgatc gcggaaatca cagcagcgga catcctgccg ggtattccgg aactcctgga
     1021 agaggcaaag aatgcaggct accgtatggc gatcgcctcc gccagcaaaa atgcgccgct
     1081 cattctggaa agactaggtc tatcggctat gttcgatgcc gtcgtcgatc cggaatcgct
     1141 gaaacagaac aaaccgcatc cagagatttt tgaaaaggct gctcaggctt tgggtctgga
     1201 aacacaggaa tgcatcggtt tcgaagactc attggctggt attgaaggga tcaaaagggc
     1261 gggtatgccc gctgtagcca tcgccatccc tatctttgct gagtggcttc cagatatcga
     1321 agtggcggct acaaaagatt tgaaattgga agaaattgta cgcaaagtga ctacgatgaa
     1381 ggagcgtgtt gaagaatggt agatttgaag gggaatcctt tttatctgaa ggatgaacaa
     1441 atagcatggg tgaaccgaac tttggaaaac ttgacgttgg atgaaaaagt aggccagctg
     1501 ttctgtccga tcggcatgtc cacggatgaa aaggcactgt ttgatttggc tgccaacaag
     1561 aaagtcggcg gactgctgtt ccgtcccgga aaaggcgaag aagtgcagca agcccaccgc
     1621 tatctgcagc accaagctaa ggttccgatg ctgatcgcag ccaacctgga agcgggcggt
     1681 acgggaagcg ctattgacgg tacgcgcatt ggcaatccga tgttgttggc ggctggtcag
     1741 gatgccgaat ttgggcacca tcttggtgaa gtatgcgcca aagaaggttc ggcggtaggc
     1801 gtgaactggg ccttcgcgcc ggtcgtggat atcgactaca attttcataa tccgatcacg
     1861 aatgtgcgca cgtacggcag tgatcccgaa accgtcctca actatgcaaa aggctttgtt
     1921 gaagccgtgc agcaaaacgg agtagcggct tcgatcaagc attttcccgg ggatggcatc
     1981 gatttccgtg atcagcatct ggtgacgaca gtcaacactt tgctgcttga agaatggcgg
     2041 agtacctatg gtaaagtcta ttcgggcctc atcgaagcag gctcattatc ggtgatgatc
     2101 ggccatatcg ctttgccggc ggtccagaaa tcagaagaag atgcttttct gccggccacc
     2161 ttatctcctg ttatcatgca ggaactgttg cgcgaggaac tcggattcaa tggcgtcatc
     2221 acaagtgatg cgacgccgat ggtcggtttt tgcgcggcga tggagcggcg tttggccgtt
     2281 ccgacagca
//
LOCUS       DAOMUJ010000295         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13384_length_2289_cov_2.014324, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000295 DAOMUJ010000000
VERSION     DAOMUJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13384_length_2289_cov_2.014324"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2289
                     /locus_tag="PLT73_07280"
     CDS             <1..>2289
                     /locus_tag="PLT73_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRM20216.1"
                     /translation="QLTQNKAELVQQLRNDYINKLQEQSSNRNTTVHLEKEMTLATEQ
                     EHRFLSKGDELRKHLRQLKAKQQALNEEYVTFVEENANLESELQDCKQNSFQLVSKLQ
                     QLNAELDTVTRNLQQAEARRESQQELDDSYASYYQGVKEMLRRRESVPGIRGPVAELI
                     QVPEKYTLAIEIALGATMQHIVVADERTASQCIGILKAQHLGRATFLPMTVIQGKSLP
                     ASVRATLEASPGYIGVANELIGHEEMYKAIVSSLLGTTIVASNIQDGLQISKKLQSRY
                     RIVTLEGDVIHAGGSMTGGATRRQQEASLLARKNNLNKLTEYVQQLSVQYNQLKREQE
                     KWSLQQQSLQEQLDDKRQKATIKQFELQQRESSLQQVTAEIATKEKESAAHEYEKRMF
                     YEEQEDRQTRLAQAQKNLLILNKEIDQLQKDIAESSLNEEERLKLLQDVQVNRQQADQ
                     ELAVLQEQEKQLRRDVKQSTERIETEQEAISSLKGKQDQVAKLADTETLTIQEITTQL
                     KSASAEKERFDAELRQLREQKKQTEQNRALQETEQNEKNKNLQKLWSEHAKLETNAGR
                     YEIAIDHHLAHLSEEYGLSFEAAKQDHHLTQEPEEAARQVKKLKKDIEELGPINMGAI
                     EEYDRISERFIFLSEQQTDLLSARGNLLNTMNEMDDEVRLRFKETFIQIKKQFEKTFP
                     KLFGGGKATLELTDPNDLLETGIEIVAQPPGKKLQQLSLLSGGEKAFTAIALLFAIIE
                     VKPVPFCILDEVEAALDEANVSR"
BASE COUNT          749 a          545 c          577 g          418 t
ORIGIN      
        1 cagctgacgc aaaataaggc ggagcttgtc caacaactcc ggaatgatta catcaacaag
       61 ctgcaggaac aatccagcaa ccgcaacaca accgtccatc ttgaaaaaga aatgacgctc
      121 gcaaccgaac aagagcaccg tttcctttcg aaaggcgacg aactcaggaa gcatctgcgc
      181 caactaaaag cgaaacagca agcgttgaat gaggaatacg tcacgtttgt tgaagagaac
      241 gccaatctgg aatcggaact gcaggactgc aaacagaata gcttccagct cgtatccaaa
      301 ctccaacaac tgaatgcgga gttggatacc gtaacccgga atctgcagca ggcggaagca
      361 agacgtgaga gccagcagga gttggacgac agctatgcca gttattatca aggcgtaaag
      421 gaaatgctgc ggagaaggga aagtgttccg ggtatccgtg gtcccgtcgc cgaattgatc
      481 caagttccag aaaaatacac cttggccata gaaattgcac tgggagccac tatgcagcac
      541 atcgtagtag ccgatgaacg cactgcatca cagtgtatcg gcattctgaa agcccaacac
      601 ttagggcgcg ccacttttct gccgatgaca gtcatccagg gcaagtcctt gcccgcgtct
      661 gtgcgggcga cattagaagc cagccccgga tatatcggcg tcgcgaatga gctgatcggt
      721 catgaggaaa tgtacaaagc gatcgtcagc agtctgttgg ggacaacgat cgtagcgtca
      781 aatatccagg acggacttca gatttccaaa aaattgcaga gccgttatcg gatcgtaacc
      841 ctggaagggg atgtcatcca tgccggtgga tcgatgacgg gtggcgcgac gaggcgtcaa
      901 caagaagcgt ccttattggc ccgcaaaaat aatctgaata agctgaccga atacgtgcaa
      961 cagctgtccg tacaatacaa tcagctgaaa cgggagcaag aaaaatggtc gctgcagcaa
     1021 caaagcttgc aggaacaatt agatgacaaa cgccaaaaag cgacaatcaa acagtttgaa
     1081 ctgcagcaaa gggaatcttc cctgcagcaa gtgacagccg aaattgcgac gaaagaaaaa
     1141 gaatcggctg cgcatgaata tgaaaagcgc atgttttatg aagagcaaga agatagacaa
     1201 acgcgattgg ctcaagccca aaaaaatttg cttatcctca acaaagaaat cgatcagctt
     1261 caaaaggata tagcggaatc cagcttgaat gaagaggaac gcttgaaact gctgcaggac
     1321 gttcaggtga accgccagca agcagatcag gagcttgctg tcctgcaaga gcaggaaaag
     1381 caattgcggc gtgatgtgaa acagtctacc gaacgcatcg aaacggaaca ggaagccatc
     1441 agctcattga agggcaaaca agatcaagtc gctaagctgg ccgatacgga gacgctcacc
     1501 atccaagaga tcacaacgca gctgaagtcc gccagtgcag aaaaagagcg attcgatgca
     1561 gaattaagac aattacggga gcaaaaaaaa caaaccgaac aaaatcgtgc tcttcaggaa
     1621 accgaacaga acgaaaaaaa taaaaacctc caaaaactat ggagtgagca cgccaaactg
     1681 gagacaaacg ctggacgcta cgaaatcgcc atcgaccacc accttgcgca cttgagcgag
     1741 gagtacggtc tcagcttcga agctgcgaaa caagaccatc acttgaccca agagcccgaa
     1801 gaggcagccc gacaagttaa aaagctgaaa aaagacatcg aggagttggg tccgatcaat
     1861 atgggggcga ttgaggaata tgatcgcatt tccgaaagat tcattttcct ttcggaacag
     1921 caaacggatc tgctttctgc ccgagggaac ctcctgaata cgatgaacga aatggacgat
     1981 gaagtccggt tgcgtttcaa agagactttc atccagatca aaaagcaatt cgaaaaaacc
     2041 ttcccgaaat tattcggcgg cggcaaagcg acgttggagc tgactgatcc gaacgacctg
     2101 ttggaaactg gaattgaaat tgtagcgcaa ccgccaggca aaaaacttca gcaactcagt
     2161 ctgctctcag gaggggaaaa agcctttacc gcgatcgcac tcttgttcgc catcatcgag
     2221 gtcaaaccgg taccattctg tattttggat gaagtggagg ctgccttgga tgaagcgaat
     2281 gtgtcccgt
//
LOCUS       DAOMUJ010000296         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13425_length_2283_cov_1.689856, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000296 DAOMUJ010000000
VERSION     DAOMUJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13425_length_2283_cov_1.689856"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..852
                     /locus_tag="PLT73_07285"
     CDS             52..852
                     /locus_tag="PLT73_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM20217.1"
                     /translation="MLNKLKKAALVGSGLLVLAGCSSESADDASVNSEAVGAETLVVG
                     LDDTFAPMGFRDENNEIVGFDVDLAKAVGERIGAEIVFQPIDWAMKETELDSGNIDMI
                     WNGYAITPERAEKVLLSEPYIVDAQSIIVLKDSAIETKADLEGKLVSTQQSSSSVDKI
                     MEDESGIFGKLGGDLVLYPSNNNAFSDLESGRVDAIVVGEVYGRYYMKQSGKDIYRVL
                     EDNFGEDEMAVAFKKDNKELQERVDAALAEMKEDGTFDEIYDKWFYSE"
     gene            885..1520
                     /locus_tag="PLT73_07290"
     CDS             885..1520
                     /locus_tag="PLT73_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM20218.1"
                     /translation="MDLIQTILPSLLDGLKMTLTLFFIIGLSSIPLGFLVAVIRVYGP
                     KWLGFLIQIYVFIMRGTPLLLQLMFVFFGLPLIGITLDRFTAAILAYIINYAAYYAEI
                     FRGGITAVPKGQFEAISVLGIGKIRGFFKIIIPQVTKIVLPSVGNEVIALVKDTSLVY
                     VIGLGELLRAGQIAANTYASLVPFLAVGIVYLSITAVITVLLNKLESKGNF"
     gene            1537..2217
                     /locus_tag="PLT73_07295"
     CDS             1537..2217
                     /locus_tag="PLT73_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20219.1"
                     /translation="MLLNAMNLTKSYHDITVIDDFSFHIDSSEIVVLVGRSGTGKTTL
                     MRLLNNLEKADQGTISIEDALLCKQGLKGAEYVDRKKRRLYQNKIGMVFQDYALFPNL
                     SVLENLLEAPLAQKLGTREELAAKAAGLLEQMGLEDKLEAMPSTLSGGQKQRVAIARA
                     MMLNPRVLCFDEPTSALDRESSDSIGKLIQEIAAGGTGILIVTHDTEFGQQYGTRIVS
                     SSEFVKNR"
BASE COUNT          610 a          457 c          620 g          596 t
ORIGIN      
        1 cgcaaaatgg ttcgctgaat aataaaactt cttgttgagg ggaaaagtcg tatgttgaac
       61 aagcttaaaa aagccgcact ggtcggatca gggttattgg tgttggctgg atgcagcagt
      121 gagtcggctg atgatgcttc tgtcaactct gaagccgttg gtgcagaaac gttggttgtt
      181 ggtttggatg atacttttgc tccgatggga ttccgtgatg aaaataatga aatcgtcggc
      241 ttcgatgtcg acttggcgaa ggcggtcggt gaacggatcg gagctgaaat tgtttttcag
      301 ccgattgatt gggcaatgaa ggaaaccgaa ttggattccg gcaacatcga catgatctgg
      361 aatggctatg ccatcacacc ggaacgggct gaaaaagtcc tcttgagcga accgtacatc
      421 gtggatgcgc aatccatcat cgttttgaag gacagcgcaa tcgaaacaaa agcggacttg
      481 gaagggaagt tggtttcgac gcagcaatct tccagttcag tggacaagat tatggaagat
      541 gagtcaggga tatttggaaa gttgggcggg gatctggtgc tctatccttc caataataat
      601 gccttcagcg acttggaatc aggacgtgtg gatgccatcg tcgtcggtga agtctacggt
      661 cgttactaca tgaagcaatc cggtaaagac atctaccgtg ttctcgagga taacttcggg
      721 gaagacgaaa tggcggttgc cttcaaaaag gataataagg aactgcagga gcgcgtcgat
      781 gcagccttgg ccgaaatgaa ggaagacggg actttcgatg aaatttatga caaatggttc
      841 tatagtgaat aacgaaaata aagggtaaga aagaggttta agaaatggac ttgatacaga
      901 cgatactgcc atcattgctt gacggcttga agatgacgct cacgttgttc ttcatcatag
      961 ggttatccag cattccgtta ggttttctgg tcgccgtcat ccgggtttac ggaccgaagt
     1021 ggctgggttt tctgatccag atatatgtat tcatcatgag gggcacaccc ttacttttgc
     1081 aattgatgtt tgttttcttc ggtttgccgc tgataggcat cacgttggat cgttttacgg
     1141 cagccattct ggcatatata atcaactacg ctgcctatta cgcggaaatc ttccgtggag
     1201 gtatcactgc tgtaccgaaa ggacagttcg aggccatttc ggtgttgggt atcggtaaaa
     1261 tcagaggatt tttcaaaatc atcattccgc aggtcactaa aattgtgttg ccgtctgtag
     1321 ggaatgaagt gattgcgcta gtgaaagata catctttggt ttatgtcatc ggtctcgggg
     1381 aattattgcg cgcaggacag attgctgcca atacgtatgc ctcgttagtg cctttcctgg
     1441 cagtcggtat cgtctattta tcgattacgg ctgtcattac ggtgctgctg aataaacttg
     1501 aatccaaagg aaacttttag ggggggagcg gcaatcatgt tattaaatgc gatgaattta
     1561 acaaagtctt atcatgacat aaccgtaatc gatgattttt cattccatat cgattccagt
     1621 gaaatcgttg tgttggtggg tcgttccgga accggtaaga ccactttgat gcgcttgctc
     1681 aacaatctgg agaaagctga ccagggaacc atttcgattg aggatgcctt gttgtgcaag
     1741 caaggtttga agggcgcaga atatgtggac cgcaaaaaaa ggcgtcttta ccaaaataaa
     1801 atcggcatgg tgtttcaaga ctatgctctg ttcccgaacc tttcggtgtt ggaaaatctt
     1861 ctggaggcgc cgcttgccca aaaattgggt acccgcgaag aactggcggc gaaagcagcc
     1921 ggattactgg agcaaatggg attggaagac aaactggaag caatgccgtc aactttgtcc
     1981 ggaggccaga agcaaagggt cgcaatcgcc cgggcgatga tgctgaatcc gcgcgtgctt
     2041 tgttttgacg aaccgacttc tgcgctcgat cgcgaatctt ccgacagcat cggtaagctg
     2101 atacaggaaa ttgcagcagg aggaaccggt atcctgatcg ttacgcacga taccgagttc
     2161 gggcaacaat acggcacccg gatcgtatcc tcaagcgaat tcgtcaagaa ccgttaagga
     2221 tattgtgaag gctagggcat gtccgcagaa aattggacag tccaaacaaa aaacagggca
     2281 gct
//
LOCUS       DAOMUJ010000297         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13467_length_2278_cov_1.484930, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000297 DAOMUJ010000000
VERSION     DAOMUJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13467_length_2278_cov_1.484930"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..888)
                     /locus_tag="PLT73_07300"
     CDS             complement(61..888)
                     /locus_tag="PLT73_07300"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRM20220.1"
                     /translation="MDLYAIYLVSDSVGETADVVIRSALYQFETENYQLKKYSYVQTT
                     EEIDDICAKASEEGKAMIFYTFVKKEMADHMMGQIEKSGLIGVDIYSPILTGLGQLFQ
                     LPAKEKPGINRQLNEDYFNRVEAIEFSVKYDDGRDPRGIEKADIVLVGVSRTSKTPLS
                     IYLANKNVKVANIPLFPESKPPKEIFTIAPERIIGLVNSPKKLNEVRKERLKTLGLPP
                     SASYAGMERILEELEYSDKIMKQIGCEIVDVSNKAIEETADHILHYMDVMNISRKQL"
     gene            complement(904..1539)
                     /locus_tag="PLT73_07305"
     CDS             complement(904..1539)
                     /locus_tag="PLT73_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM20221.1"
                     /translation="MELTDRQKQIIEIVKKQQPISGEKIASILGFARATLRPDFSLLT
                     MSGILQAKPKVGYLINEEHTNSVLVEQIAKQKVEKVMAISVNLTKETSIQDAIIQLFL
                     EDCGSLYVTDNREILGIVSRKDLLKSALGDSDLNAIPVGMVMTKMPNLYTVFSDTLVV
                     DATKLLVTHRVDSLPVLSRPVYEETGKKEVIGKFSKSTASRLLLDIFNKDL"
     gene            1768..>2278
                     /locus_tag="PLT73_07310"
     CDS             1768..>2278
                     /locus_tag="PLT73_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRM20222.1"
                     /translation="MQEIKDMTYSNTTFQKGMQLFESGALRQYTANSAENTFYADIKG
                     TKKYEVEVYLDEYAEIDDYYCSCPAFESYPGPCKHVVAFLLAILNSSSDYRKERKTAS
                     KPTAIANKSSSYDVEQTKRLLDVLQFELLEENNLFDRVPIQVEYTMVMSDLRYGQHYS
                     LKMRVGAGQF"
BASE COUNT          545 a          530 c          581 g          622 t
ORIGIN      
        1 gccttctgaa aaacggtaaa cccattgctt cgtcatatgc ttttcctcct tgtgagtggt
       61 ctatagttgc ttgcggctga tattcatgac atccatgtag tgcaggatgt ggtctgcggt
      121 ttcttcgatc gctttgttcg aaacgtcgac gatttcgcag ccgatctgtt tcatgatctt
      181 gtctgagtat tcgagttcct caaggatgcg ctccatgccg gcataactgg ctgagggcgg
      241 cagccccagt gtcttcaggc gctctttgcg gacttcattg agttttttcg gcgaattcac
      301 caggccgatg atccgctccg gtgcaatcgt gaagatttct ttcggcggct tcgattccgg
      361 aaagagcgga atgttcgcga ctttaacgtt cttgttcgcc agatagatcg acagtggtgt
      421 cttggatgtg cgggacacgc cgaccagcac gatgtccgct ttttcaatgc cgcgcggatc
      481 gcggccgtca tcatatttga cggaaaactc gatggcctca acacggttga aatagtcctc
      541 gttgagttgg cggttgatgc ccggcttctc cttggccgga agctggaaca gttgacccaa
      601 accagtcaaa atcggactgt agatgtcaac gccgataaga ccggactttt cgatctgtcc
      661 catcatgtga tcggccattt cctttttcac gaaggtgtaa aagatcattg ccttcccttc
      721 ctcggaagct tttgcgcaga tatcgtcgat ttcctcggtc gtttgcacgt aggaatattt
      781 cttcagttgg taattttccg tctcgaattg gtagagggcg gaacggatca ctacatcggc
      841 cgtctcgccg acggaatcgg atactaagta aatcgcgtaa aggtccattt gcacactgtc
      901 ctcctaaagg tctttgttga atatatcaag caacaatcta ctggcggtcg atttcgaaaa
      961 ttttccgatg acctcttttt tgccggtctc ctcataaaca ggacgggaaa gcaccggcaa
     1021 actgtcgacg cgatgggtga cgagcagttt cgtggcatcg acaacgagcg tgtcgctgaa
     1081 gacggtatat agattcggca tcttcgtcat caccattccg accgggatgg cattcagatc
     1141 ggagtcgccc agagcgcttt tgagcagatc tttgcgggaa acgatcccga gtatttcccg
     1201 gttgtcggtg acataaaggg aaccgcaatc ctcaaggaaa agctggatga tggcgtcctg
     1261 gatgctcgtt tctttggtga ggttgaccga gatggccatt accttttcga ctttttgttt
     1321 ggcgatctgt tcgaccagca cggagttggt gtgctcctcg ttgatcagat aaccgacttt
     1381 cggtttggcc tgtaggatgc cgctcattgt cagcagcgaa aagtcgggcc gcaacgtcgc
     1441 tcgtgcaaat cctagtatgg aggcaatctt ttcaccggaa atcggctgtt gtttcttgac
     1501 gatttcgatg atctgttttt gcctgtcagt gagttccatt tttacacctc aattcttaat
     1561 gtgatgtgtt aatgaatata tagtatgtca caatgagggt gttgtcaaca caaagtgaca
     1621 tctttttgct gggatgtatc ggacattctg tcccgtccga caatccagga gccatttttg
     1681 tagcctgatc aacaacggtc tatggtaaac ttataaagac taagcgagcg aggatgtgga
     1741 ccgagaatga tttcagtagg atacaatatg caagaaataa aggatatgac atacagcaac
     1801 acgaccttcc agaaagggat gcaattgttt gaaagcgggg cactgcggca atacacagcc
     1861 aacagtgctg aaaatacctt ttacgcagat atcaaaggga caaaaaagta cgaagtagag
     1921 gtttatctgg atgaatatgc agaaatcgat gattactatt gttcctgccc ggccttcgaa
     1981 agctatccgg ggccttgcaa acatgttgtg gccttccttt tggcgatcct caattcttct
     2041 tcggactaca ggaaggaacg gaaaaccgca agtaaaccaa cagctatagc caataagtcc
     2101 agcagctacg atgtcgaaca gacgaaacgt ttgctggacg tgcttcaatt cgaattgctt
     2161 gaggagaaca acctgttcga cagggtgccg atccaagtgg aatatacgat ggtcatgtcc
     2221 gacctgcgct atggccaaca ctacagcctg aagatgcggg taggcgccgg gcagtttt
//
LOCUS       DAOMUJ010000298         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13482_length_2276_cov_2.061684, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000298 DAOMUJ010000000
VERSION     DAOMUJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13482_length_2276_cov_2.061684"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLT73_07315"
     CDS             complement(<1..800)
                     /locus_tag="PLT73_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM20223.1"
                     /translation="MQIAELYDTVKTIRRELHQIPEIGFDLPLTSEYVRQKLEAFGYE
                     PISTAETGWVAVLEGKSPAAVAFRADMDALEVTEETGADFASLHPGKMHACGHDGHMA
                     LLLGFAKYLKSLPMLEKSVVLVFQPAEEGPGGAKWIMDSGILQELQVEKIYGYHLYPS
                     LPEGILGLAKGPLMARNGEFDITLEGVSSHAGQPHFGKDALIAAAQILLSVQNILARD
                     LDPLQPAVVNIGTLHAGEARNIVAGKAELTGTIRSYDKAVYASIKERLA"
     gene            complement(832..>1352)
                     /gene="dapD"
                     /locus_tag="PLT73_07320"
     CDS             complement(832..>1352)
                     /gene="dapD"
                     /locus_tag="PLT73_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HRM20224.1"
                     /translation="NKDKIKDQVMEYDRRNSAIPLLDTTKIDARIEPGSFIRDHVTIG
                     KSAVIMMGAVINIGAVIGEGTMIDMGAVVGARGTIGKNCHIGAGAVVAGVLEPPSKSP
                     VIIEDGVLVGANAVIIEGVHIGEGAVVAAGAIVLEDVPANAVVAGSPAKIVKMKDEKT
                     SEKTQLLADLRD"
     assembly_gap    1353..1362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1363..1540)
                     /locus_tag="PLT73_07325"
                     /pseudo
     CDS             complement(1363..1540)
                     /locus_tag="PLT73_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128763.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
     regulatory      complement(1701..1846)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT          487 a          621 c          554 g          604 t
ORIGIN      
        1 gcaaggcgtt ccttgatgga agcatagacc gccttgtcgt aactccggat cgtccctgtc
       61 aattcagcct ttccggccac gatattgcgg gcttccccgg catgcagcgt accgatgttc
      121 acgacagctg gttgcagcgg atcgagatcg cgggccagaa tgttctggac cgaaagcagg
      181 atctgcgcgg cggcgatcaa ggcatccttg ccgaagtgcg gctgcccggc atgcgagctg
      241 acgccttcaa gcgtgatgtc gaattcaccg ttgcgcgcca tcaatggacc cttggccaag
      301 ccgagaatac cttccggcag gctcggatac aggtggtaac cgtagatttt ttcgacctgc
      361 aactcctgca ggatcccgga atccatgatc catttcgcac cgcctggccc ttcctcagcg
      421 ggttggaaga ccaaaacgac tgatttttcc agcatcggca gggacttcag gtatttcgcg
      481 aaacccaaca acagggccat atgcccatcg tgtccgcacg catgcatttt tccagggtga
      541 agggaagcga aatccgctcc agtttcctcg gttacctcaa gggcatccat gtccgcacgg
      601 aaagcaaccg ctgccgggga cttaccctcc agaactgcta cccagcccgt ttcggcggtg
      661 ctgatcggtt cataaccgaa cgcctccagc ttctggcgga cgtattccga agtcagcggc
      721 agatcgaaac caatttccgg aatctgatgg agttcccttc ggatcgtttt caccgtatcg
      781 tataattctg caatctgcat gctgttcacc tggtccctcc gcgtgtagtt attagtctct
      841 taagtcagcc aataattgag ttttttcgct tgtcttttcg tccttcatct tcacgatttt
      901 tgcaggggat cccgcaacta ccgcgttagc cggtacgtct tccaatacga tcgcgccagc
      961 tgcgacaaca gcgccttcgc cgatgtgcac gccttcgatg atgacagcgt tcgcgccgac
     1021 caaaacaccg tcttcgatga tgactggtga tttcgatggt ggctcaagaa cacctgcaac
     1081 cactgcgcct gctccgatgt ggcagttttt gccgatcgtg ccgcgtgcgc cgactaccgc
     1141 gcccatgtcg atcattgtgc cttcgccgat gacggcgccg atgttgatga ccgcacccat
     1201 catgatgaca gcgctcttgc cgattgttac atgatcacgg atgaatgagc caggctcgat
     1261 gcgtgcatcg attttggtag tgtccaacag cggaatcgca gaattgcgtc tgtcgtattc
     1321 catgacttga tccttgatct tgtctttgtt tgnnnnnnnn nnaaatcgct gaagcgtctc
     1381 cgaagactgt gtagctgtct ccgcttccga agaatttcaa gccttcgtag gcaggatttt
     1441 caaaatcccc cttgatgtaa agttttatgg gtgtttgctt ttcggcagct ttgatgtaag
     1501 ccgcgatttc ataagcatct gtcaataata gttcgctcat tttgctgttt cctcccgatc
     1561 attattttct gttccaacat gcccattata gcatgccaac ctgtttttgc cctatgcggc
     1621 ggacgaccgt ttgttcctga aatgcaaaaa aaatagcggt aagcgagtgc ttacggctac
     1681 ggagacctgc attcgcccaa ccaagctaaa gcgcaatcct ttgtcatcgg gcaacgacaa
     1741 tggaacagcc ccacatttgt tcaacctggg tccagcattc gcatgcttcg gcgaccgtcc
     1801 ctttcccttg cggtactctt gaccagccgc gacctctttt cgcttggcga ttcatttatt
     1861 tttctaatcg tacacttatc cggcaatcct gtcaaccgct ctggcacaga agcttctttc
     1921 cgcttgtcct ttgcgtgttt ttgaggtgat tgtttggata cgaacggggc tccggatcgg
     1981 aaagtgccgg gaaggatcgg ccgcccctat gtgaaaacgc tcattaaact cgcggatttc
     2041 taattttcac cccggagtat acaaatttgc atagaaatac gcctgcaatc gtgttatgtt
     2101 attttcatga gtttggacgt cagcagtcgg ttggcgtatg ttcggctata cattagatca
     2161 aggatatttc atctggagga gataataaat tatgagtaga gaaacgaatc gttttgtcaa
     2221 gcaactgaaa ggatcgccca tccgtaactt cgacgccgcc atcagtgatg tgaagg
//
LOCUS       DAOMUJ010000299         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13489_length_2275_cov_2.843243, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000299 DAOMUJ010000000
VERSION     DAOMUJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13489_length_2275_cov_2.843243"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1958)
                     /locus_tag="PLT73_07330"
     CDS             complement(48..1958)
                     /locus_tag="PLT73_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM20225.1"
                     /translation="MIKWINKYKNRITALSGFLIVIGFALKTLGYTTAADFALILATL
                     IAVVPIMYKAYLALRMKAFSIELLVTIAVIGALFIREYTESSVVTFLFLFGDFLEART
                     LEKTRSSLRSLIDMAPQEAIVLHGTDQVVVPVEEVAVGDRLFIKPGGKIPVDGIIVKG
                     QAAINEAAVTGEAIPANKSLDDKVFSGTLVDDGFIEMIAEKVGDDTTFAKIIELVEEA
                     QESKSKAERFLDAFANFYTPAIVVLSVIVYVWTRDLHLAITFLVIACPGALVIGAPVS
                     NVAGIGNGAKYGVLVKGGEVMDRFSKVDTLVFDKTGTLTKGKPEVTDIHVLSGADRSE
                     LLQLTASAERISEHHLGQTIVKYAHQQGLETSVAPKDAEVIKGHGLRATVEGQRLVAG
                     NRKLMVQSGILINEAAEIYAIAREKTGNTVVFVAIDGTLAGLFSIADQIREDAPQALA
                     EMRRNGIKQIIMLTGDNRHTAQAVAEQLGLDAFHAELLPEDKVRFVKQLQADGAVVAM
                     AGDGINDAPAIATADIGLAMGEGGTDISMETADVVLMADKLMQFSHAYSLSKATMRNM
                     KQNIAIAVGVVFFLLAGVLMGSVHLASGMFIHEASILAVILNAMRLIGFNRKKSGTGS
                     FSHERNLGNRAD"
BASE COUNT          496 a          639 c          610 g          530 t
ORIGIN      
        1 aacctgctga cgcaggaagt ctctcagcat attgcgctct acgatgctca gtcggcacga
       61 tttcctaaat ttctctcatg gctgaacgaa cccgttccgc ttttcttccg gttgaagccg
      121 attaaacgca tagcgttcag gatgacggcc aggatgctgg cttcatgaat gaacataccg
      181 ctggcgagat ggacggaacc catcagcacg cccgccaaaa ggaagaacac gactccaacc
      241 gcaatggcga tgttctgttt catgttgcgc atggtcgcct ttgagagcga ataagcatgg
      301 gaaaactgca tcagtttgtc ggccatcagc acaacatcgg ctgtctccat cgagatgtcg
      361 gtcccgcctt cgcccatggc caagccgatg tcggctgtcg cgatggccgg tgcatcgttg
      421 atgccgtcac cggccatcgc cacgacggcg ccatctgcct gcagttgttt gacgaaacgc
      481 actttgtcct ccggcaacag ctcggcatgg aaagcgtcca atcccaattg ttcggcgacc
      541 gcttgggcgg tgtggcggtt gtcccccgtc agcataatga tctgtttgat gccgttgcgg
      601 cgcatttcgg ccaaagcctg cggtgcgtcc tcgcggatct gatcagcaat ggagaagagc
      661 ccggcgagcg tgccgtcaat ggccacgaaa acgacggtat tgccggtttt ttcgcgtgca
      721 atcgcgtaga tttctgctgc ttcatttatc aagattccgg actggaccat cagtttgcgg
      781 ttgccggcga cgagacgttg accttcaact gtcgctctca atccgtggcc tttgatgact
      841 tccgcatctt tcggcgcaac agatgtttcc agcccttgtt gatgtgcgta tttcacgatt
      901 gtttgtccga gatggtgttc ggaaatgcgc tccgccgaag ccgtcagctg cagcagttcg
      961 ctgcgatcgg cgccactgag gacatggata tcggtcactt ccggtttgcc tttcgtcagc
     1021 gtgccggtct tgtcgaaaac aagtgtatcg actttggaaa agcgatccat gacctcgcca
     1081 cctttcacca gcacgccgta tttggcgccg ttgccgatgc cggccacgtt cgataccgga
     1141 gcaccgatca caagcgcacc cgggcaggcg atcacaagga aagtgatggc aagatgcagg
     1201 tcgcgcgtcc aaacgtaaac gatgacggag agcaccacaa tcgccggcgt gtagaagttg
     1261 gcgaaggcat ctaggaaacg ttcggccttg gatttggatt cctgcgcctc ttcgaccaat
     1321 tcaataattt ttgcgaaagt tgtgtcatcg ccgacctttt ccgcgatcat ttcaatgaag
     1381 ccatcgtcca ccaatgtacc cgaaaagact ttgtcatcca aggatttatt ggccgggatc
     1441 gcttctcccg tcacagcggc ttcgttgatg gccgcctgac ccttcacgat gatgccgtca
     1501 accggaattt tgccgccggg cttgatgaac agccgatccc cgacagccac ttcctcgacc
     1561 ggcacaacga cttgatcggt gccatgcagg acgatcgctt cctggggcgc catgtcgatc
     1621 aacgaccgca gagaagagcg ggtcttttcc aaggtgcgtg cttccaggaa gtcaccgaac
     1681 agaaacagga aggtgacgac ggaagactcc gtatattcgc ggatgaacag cgcaccgatg
     1741 acggcaatcg tcaccagcag ctcgatgctg aaagccttca tccgcaacgc cagataggct
     1801 ttgtacatga tcggaaccac cgcgatgagc gtcgcgagga taagcgcgaa gtcagcagca
     1861 gtggtatatc cgagggtttt caaagcaaaa ccgatgacga ttaggaagcc tgatagtgcg
     1921 gtaatgcggt ttttgtattt atttatccat ttgatcattt gaatgacctc ctctggtatt
     1981 ttcctatagc ctaatcatac gccatcaggc aggaaagcga cttgatgggg gtcaagcttt
     2041 ggatgcgcaa tttcgctcag cagaatctca accgttttcc tccggcgaac ggaggctacg
     2101 ccggagtacg gcccacattt aagtcttgtg ctccgacgaa accctgcccg caggagaaga
     2161 gcccgcaagt taccgctgtt ctccggtgtg ggaagcctcg ccggaggaac gcaccgattc
     2221 tgcactttca actccggtca gagacgcttc accgttcatt tttggttgtt ctgcc
//
LOCUS       DAOMUJ010000300         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13496_length_2274_cov_2.814781, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000300 DAOMUJ010000000
VERSION     DAOMUJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13496_length_2274_cov_2.814781"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..787)
                     /locus_tag="PLT73_07335"
     CDS             complement(26..787)
                     /locus_tag="PLT73_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRM20226.1"
                     /translation="MFRIKNFEDNNDVKIVEELGAFKVVEYLKDLSVDHLTAQAAYYA
                     SEMNVRRRQLVCDVSLSDITVQSGAMQWMVGNVSATTGLKGVGDFLGKALRGKVTNES
                     AIKPEYTGTGKLVLEPTYKHILLIDVDDWNGSIVIEDGLFLACDSELNHKAVMKSNLS
                     SAAMGGEGLFNLGLSGGGIVALESYVPREELIEIELENDELKIDGNMAIAWSGSLEFT
                     VSRSGQTLLGSAASGEGLVNVYRGTGKVLMAPVLR"
     gene            complement(1043..2239)
                     /locus_tag="PLT73_07340"
     CDS             complement(1043..2239)
                     /locus_tag="PLT73_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HRM20227.1"
                     /translation="MLEFKQNIRGNVALGVNPLGCKQEVLNEIDYVKNKGTFAGPKKV
                     LIIGASSSYGLATRISLAFGAGADTIGVSFEKGPKNERNLGTAGWYNNIAFREAAEKE
                     GLIAKNFVQDAFSHESKQEVIAYIKNEFGGKVDLVVYSLASGRRTDPDTGETYTSAIK
                     SLGEPVVGPNINMQNHAFYTETLEPATAEEIAGTVKVMGGEDWQLWMEALKEADVLAD
                     GVLTTNYSYLGTELNRPYYGGGTLGLAKADCDEKAKTINGLLADINGKAQIVVATAVT
                     TKASSVIPFFPVYCIGLYKVMTEKGTHETPIMHIDRIYRDMVYGENAEYDEAGRLRPD
                     SWELDSDTQAKTKALIQQLNKENFNTDMAAYDLLYKEFSILSGFMVDNYVEQDVTIEE
                     LKALEY"
BASE COUNT          549 a          564 c          481 g          680 t
ORIGIN      
        1 caagcgatcg ataggtcatg tttttttacc ggagaacggg cgccatcaaa actttgcctg
       61 ttccacgata gacattgacc aaaccttcgc cggatgcggc tgaacccaga agtgtctggc
      121 ctgatcttga tactgtaaat tccaggctgc cggaccaagc gatcgccata ttcccatcaa
      181 tcttcaattc atcattttcc aactctattt ctatcagttc ttctcttggt acgtacgatt
      241 caagtgcgac aatcccgccg ccagaaagac ctaggttaaa aagaccttct ccgcccatcg
      301 cagcagaaga gaggttggac ttcataactg ctttgtggtt caattccgaa tcgcaagcca
      361 aaaataatcc gtcttcgatt acaattgagc cattccagtc atcgacatca atcagcaaaa
      421 tatgcttata ggtcggttcc aataccagtt ttccggttcc ggtgtattca ggcttaattg
      481 cggattcatt cgtgactttc cctcgaagcg cttttcccaa aaaatcgccc acgcctttaa
      541 gcccggttgt agcactgaca ttcccgacca tccactgcat ggctcctgat tgaacggtga
      601 tgtcggaaag gctgacatcg cagaccagct gtcttcttct gacattcatt tcagaagcat
      661 agtaggcagc ttgagctgtc aggtgatcga cactcaaatc tttcaagtat tccactactt
      721 taaaagctcc cagctcttca acaatcttca cgtcattgtt atcttcgaaa ttttttattc
      781 tgaacatatc cattttcctt ctttcttttg cagaggcaat agctgtgtct ggtcaataaa
      841 ggcttattca aatttggtgt atcattgtct atcattataa cactattttg attatcgacc
      901 aatatttaga tggaaacggt aaccggatgg aagaagaacg cttgtgttgt cagccgtaga
      961 agcagaaaag tcctgactct cagagctagg acttgtcatt catcggtgcg gataaaaggt
     1021 gaattggtgt caccggaacg atttaatatt ccaaagcttt caattcttcg atcgtaacgt
     1081 cttgctcgac atagttatca accatgaatc cacttaaaat tgaaaactct ttgtaaagaa
     1141 gatcatacgc cgccatgtcg gtattgaaat tctctttgtt caattgttgg atcagtgcct
     1201 ttgttttagc ttgcgtgtcg ctgtcgagtt cccagctatc cggcctcagg cggccagctt
     1261 catcatactc ggcgttttcg ccataaacca tatcgcgata aatgcggtcg atgtgcatga
     1321 tcggtgtttc gtgggtgcct ttttcggtca tgactttgta gagaccgata caataaacag
     1381 ggaagaacgg gatgacggag ctggctttgg tggtcacggc agtagctacc acaatctggg
     1441 cttttccgtt gatgtcagcc aataatccat tgatggtctt cgctttttca tcacaatctg
     1501 ctttggcaag gccaagagta ccgccaccgt agtaaggacg gttcagctct gttcccaaat
     1561 aagagtaatt ggtcgtcaga acgccatctg ccaatacatc ggcttctttc aatgcttcca
     1621 tccatagctg ccagtcttcg ccgcccataa ctttgacggt tccggcgatt tcttctgctg
     1681 ttgcaggttc cagcgtttcc gtgtagaaag cgtgattttg catattgatg ttcggaccga
     1741 ccactggctc accaagcgat ttgattgcgg atgtgtacgt ctctccggta tcgggatcgg
     1801 ttcttcggcc gcttgccagg ctatagacga ccaggtccac tttgccgcca aactcgttct
     1861 tgatgtatgc gatgacttct tgcttgcttt cgtgactgaa ggcgtcctgc acaaagttct
     1921 tggcgatcag gccttctttt tcggcagcct cacggaacgc gatattgttg taccagccgg
     1981 ctgtgccgag attccgttca ttcttcggtc ctttttcgaa agaaacgcca atcgtatctg
     2041 ctcccgcacc gaaagccagg ctgattcggg tagctaaacc gtagcttgat gacgcaccaa
     2101 tgatcaagac ttttttcgga cctgcaaagg tccctttgtt tttgacatag tcgatttcat
     2161 tcaatacttc ctgcttgcat ccaagcgggt ttacaccaag ggccacatta ccccgtatat
     2221 tttgtttaaa ttccaacatg attaattttc ctccattaaa atgtcataag gata
//
LOCUS       DAOMUJ010000301         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13501_length_2274_cov_2.162235, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000301 DAOMUJ010000000
VERSION     DAOMUJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13501_length_2274_cov_2.162235"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLT73_07345"
     CDS             <1..799
                     /locus_tag="PLT73_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM20228.1"
                     /translation="NNCHEKLPHVKIGPAPNISAVYPKSNNPADVQAADLYTAIRNWL
                     YLDIAVYGVYNHQVWAYLEENEALPDMEPGDLEILKSAECDFIAFNYYNSSTVQAYPA
                     GTERATGKKDQQLSDSQEGFFAGSDNEHLPYTEFGWQIDPTGFRTTINQIYSRYRKPL
                     IVTENGLGGIDKLEDGKVHDNYRIDYLRNHIEQMNLAVTDGADVFGYCTWSAIDLIST
                     HQGFRKRYGFVYVNREDFDLKDLKRYRKDSFYWYKGVIASNGGKLEG"
     gene            996..>2274
                     /locus_tag="PLT73_07350"
     CDS             996..>2274
                     /locus_tag="PLT73_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM20229.1"
                     /translation="MDKFLEFLEGKLGPIAYKLNANRYLSAIKEGFFGAMSLLIIGSM
                     FLLVANLPIPGYADFMAGVFGADWTQFFMVPFDMTMNIMTIFVMIGMAKDLCKTYGID
                     DVAGIIYALVGFLILTPSILSTDNAAGIPMGNLSASGLFLGMMSTVLAVEIVRFVLGR
                     GWTIKMPDSVPANVARSFDALIPGLFVILVFDILKLIFAMTSFETAQAFIFEIIQAPL
                     TSIGATLPATILVVILETLLFSFGLHGPNILGAVMNPIWLTLTAENASAYAAGEVLPN
                     IVNAQFYANFIKIGGAGATFGLALLCLFVAKSSQFKTLGKLAIGPAIFNINEPLIFGM
                     PIVLNPILMIPFIVSPVVMTTLTYILMNIGLIPLTNGVNIPWTTPPIISGFLISGWQG
                     AVWQVIEMGLSALIFYPFFKLEDNKAYQIEMGQI"
BASE COUNT          657 a          464 c          506 g          647 t
ORIGIN      
        1 gaacaactgc cacgaaaaat taccgcatgt gaaaatcgga ccggcaccga acatttcggc
       61 ggtctatccg aaatcgaaca acccggcgga cgtccaagca gccgatctgt ataccgcaat
      121 ccgtaactgg ctttatcttg atatcgccgt gtatggtgta tacaatcacc aagtgtgggc
      181 ttacctggaa gaaaatgagg ctttgcctga tatggagccg ggtgacttgg agatactgaa
      241 atccgcggaa tgcgacttca tcgccttcaa ttactacaac agctcgactg ttcaagccta
      301 tccagccgga acggaacgcg caacaggcaa aaaagatcag cagttatcag atagccaaga
      361 aggattcttc gcaggatccg acaatgagca tcttccttat acagaattcg gttggcagat
      421 cgaccctact ggattccgca cgacgattaa ccaaatttac tctcgttatc gcaaacccct
      481 gatcgttact gaaaacggtt taggtggtat tgataagctg gaagatggga aggtccatga
      541 taactatcgg atcgactatc tccgcaacca tatcgaacag atgaacctgg cagttacgga
      601 tggagcagat gtgttcggct actgcacctg gagtgcaatc gacctcatca gtacccacca
      661 agggttccgc aagcgctatg gctttgttta cgtcaaccgg gaagacttcg atctcaaaga
      721 tctgaagaga taccggaaag atagtttcta ttggtataaa ggagtgatcg cgtcaaacgg
      781 aggaaaactg gaaggttaga ggtagaaagc aagaatagtt tttcgcacca tccattgttg
      841 ataaagaaca gcggcaagtg aatgcggaaa acgaaggccc attgttctga gactgactgg
      901 cagaatccgg acaagcaatc atatcaattt attttactgt tttcgaagac ggcacgctgt
      961 cttcacaagt gttcctaaca gaggaggatt ttattatgga taaattctta gaatttttag
     1021 aaggaaaatt gggtcctata gcgtataaat taaatgcaaa ccgttactta tcggcaatta
     1081 aagaaggttt cttcggagcg atgtcactat tgatcatcgg ttccatgttc ctattagttg
     1141 ctaacttacc aattccagga tacgctgatt tcatggcagg tgttttcggc gctgactgga
     1201 cacaattctt catggttcca ttcgatatga ccatgaacat catgacaatc ttcgttatga
     1261 tcgggatggc gaaagattta tgcaaaacct atggaattga tgatgtagca gggattattt
     1321 atgcgcttgt tggattcctt attttgacac cgtcaatctt aagcaccgat aatgcagcag
     1381 gaatcccaat gggaaacttg agtgctagcg gattgttctt aggtatgatg agtactgttt
     1441 tggcagttga aattgtacgt ttcgttttgg gcagaggatg gacaatcaaa atgccagatt
     1501 cggttcctgc aaacgttgcg agatcatttg atgccttaat acctggcttg tttgttatct
     1561 tggtattcga tattttgaaa ttgatttttg ctatgacatc atttgaaaca gcccaagcat
     1621 tcatttttga aattattcaa gcaccattga ctagtattgg tgccacgtta ccagcgacaa
     1681 tccttgtagt catcttagaa acactattgt tctcattcgg cttgcatggt ccaaatatct
     1741 tgggtgcggt aatgaaccca atttggttga cactgactgc tgaaaatgct agtgcatatg
     1801 cagcaggaga agtattacca aacatcgtca atgctcaatt ctatgcaaat ttcattaaaa
     1861 ttggtggtgc tggagcaact tttggactag cgttattgtg tttattcgtt gcaaaatcga
     1921 gccaattcaa aacgttgggt aagttagcaa tcggacccgc aatatttaac atcaacgaac
     1981 cgttgatctt tggtatgcca atcgtattga atcctatctt gatgattcca ttcattgtgt
     2041 caccggtagt catgactacc ttaacgtata tcttgatgaa cattggcttg attccactta
     2101 caaatggggt aaacatacct tggactacac caccaattat ttctggattc cttataagcg
     2161 gctggcaagg tgctgtatgg caagtaatcg aaatgggctt aagtgcttta atcttctacc
     2221 cattcttcaa attggaagat aacaaagctt accaaatcga aatgggtcaa atcg
//
LOCUS       DAOMUJ010000302         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13518_length_2272_cov_2.126297, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000302 DAOMUJ010000000
VERSION     DAOMUJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13518_length_2272_cov_2.126297"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="PLT73_07355"
     CDS             complement(<1..2258)
                     /locus_tag="PLT73_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20230.1"
                     /translation="MRPLKLEMNAFGPYKEKIVLDFTQFQNQTLFLVSGPTGAGKTTI
                     FDAIAYALYDVASGSSREKDTFKSQFATEESICYVDLTFEYNGKSYRVRRSPAQTGPG
                     KSGKIIDLKADVAFYHDDTVTTKARDANAEIEQLLSLNYEQFKQIVMLPQGEFKKLLE
                     SNSNDKETIFRNIFGTEILKRFQEELKERAKSLQSQATSAHDSLQTAYSFASGIKDEA
                     LQEALRLQDTEQILVRIADLLAALEEEREVKELQLTSLRSQSQAVSDTIKLLEDIAQL
                     EVRLKVLEEEEAQALGWKQQLERHEQAVKAEEKGSLYIAASTNRAAKQEQSKQNDALL
                     DQLNQASRDKQSAFEVAELAFSLIPGLREKIGTVKEQEKQLIALAKKQKELAGYQKAG
                     NDATKELQKHANLKQAQKEKQEQCQQRLAGLKQAQKEAASIKDKLSACRMNVQQSDQI
                     HKRIVQTEKLLAEKLAKGARFLILEKEFMAIDQQFKDEILLYNRNIAGILAEQLISGE
                     ACLVCGSTDHPAPAQKTAETLSDQDREQLEKKRNQAYADYQQGTAELSGIRKQLADLF
                     DEFAIPAETFETYKQEQKAKRDSEIASEKTLLSEMIALQKILDEEETLVKQQNNIETN
                     LQEIDQNCLTLQKTLHLNEILSRDLLADIEQEQQILGDKDLATVQNEILQLNETIETS
                     TNNYNRLNSELTDLEKRIAVCHANSTTYAAQVEEATHALLDAETTYKTVLADTGLEEP
                     FDHLILDKALSL"
BASE COUNT          430 a          553 c          555 g          734 t
ORIGIN      
        1 tccagagaca atgccttatc caatattaga tggtcgaaag gttcttccag tcctgtatca
       61 gccagtacgg ttttgtaggt tgtttccgca tccaacaaag catgggttgc ttcctcaact
      121 tgggccgcgt aggtggtgct gttggcatgg cagacggcaa tccgtttctc gaggtccgtc
      181 agctcgctgt tcagcctgtt gtagttattt gtgctggtct caatggtttc gttgagctgc
      241 aggatttcat tttgcacagt tgcgaggtct ttgtcgccca aaatttgctg ttcctgttcg
      301 atatcggcca gcaggtccct ggaaagaatt tcgttcaagt gcaatgtttt ctgaagcgta
      361 agacagttct ggtcgatctc ctgcagattt gtttcgatgt tgttttgctg cttgacgagg
      421 gtctcttctt cgtcaagaat cttctgcaga gctatcatct ccgacagcag cgttttttct
      481 gacgcgattt cgctatcgcg ttttgctttc tgttcctgtt tgtaagtttc gaaagtttca
      541 gccgggatag cgaactcatc gaagagatca gccagttgtt ttcggattcc ggaaagttcg
      601 gcagtcccct gctggtaatc cgcatatgcc tgattccgtt ttttctccag ctgctcccgg
      661 tcctgatcgg agagggtctc tgccgttttt tgagcgggtg cgggatggtc tgttgaaccg
      721 cagacaagac aagcttctcc gctgatcagc tgttctgcta aaatgcctgc gatgttgcgg
      781 ttgtagagca ggatttcgtc cttgaactgt tgatcgattg ccatgaattc tttttccaga
      841 atcaggaatc gtgcaccttt tgcaagtttt tcagccaaca atttttcggt ttggacaatc
      901 ctcttgtgga tctgatcgga ttgctgcacg ttcattcggc aagcactcaa tttgtctttg
      961 atactggcag cttctttttg ggcttgtttc agccccgcaa gcctctgctg gcactgctcc
     1021 tgtttttctt tttgcgcttg tttaagattt gcatgtttct gcagttcttt cgtggcatcg
     1081 ttgcctgctt tctggtaacc tgccagttct ttctgcttct tcgccaaggc gataagctgt
     1141 ttttcctgtt ccttcacggt gccgatcttt tcacgcaagc ctgggatcag ggaaaaggca
     1201 agttcagcca cctcgaatgc cgattgctta tcgcgtgaag cctgattcaa ttggtccaag
     1261 agagcgtcat tttgtttgga ttgttcctgt tttgcagccc tgtttgtgga tgctgcaatg
     1321 tacagcgacc ctttttcctc ggctttaacg gcttgttcat ggcgctccaa ttgctgcttc
     1381 catcctaagg cttgagcttc ttcttcctcc agaactttca gccgtacctc caactgtgcg
     1441 atatcttcca gcaatttgat ggtatcggaa acggcttgac tttgtgaacg caaagaagtt
     1501 agctgcagtt ctttgacttc acgttcttct tcgagcgccg ccaacagatc tgcgatccga
     1561 accaggatct gctcggtgtc ctgcaagcgg agcgcttctt gcaaggcttc atctttgatt
     1621 cccgaggcaa aggaataagc ggtctgtagc gaatcatggg cggaagtcgc ctggctctgt
     1681 aatgatttgg cccgctcctt cagttcctcc tggaatcttt tcaaaatctc ggtcccgaag
     1741 atgttccgga aaatagtctc tttgtcattg ctgttcgatt ccagtagttt tttgaattcc
     1801 ccttgcggca gcatgacgat ctgtttgaac tgctcgtagt tcaaagaaag caattgctct
     1861 atttccgcat tggcgtcgcg cgctttcgtc gtcacggtgt cgtcgtgata aaaggcgaca
     1921 tcagctttca aatcgatgat ttttccgctt ttcccaggac ctgtctgggc aggggagcgt
     1981 ctgacgcggt aagatttccc gttgtattcg aacgtcagat cgacgtaaca gatcgactct
     2041 tccgttgcga actgcgactt gaaggtgtcc ttttcccggc tgctgccgct ggcgacatcg
     2101 taaagggcat aggcgatggc atcaaaaatt gtggttttgc cggcaccggt aggcccgcta
     2161 accaggaaca atgtttggtt ctggaattgc gtgaagtcca atacgatctt ttctttgtag
     2221 ggaccaaagg cattcatctc taattttaag ggtctcatct aatctttcct tc
//
LOCUS       DAOMUJ010000303         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13544_length_2268_cov_2.170809, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000303 DAOMUJ010000000
VERSION     DAOMUJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13544_length_2268_cov_2.170809"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLT73_07360"
     CDS             complement(<1..241)
                     /locus_tag="PLT73_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM20231.1"
                     /translation="MYHKKLKPFKEGFLWGSATSAYQVEGAWDEDGKGPSVQDVKEIV
                     PDTSDFKVATDHYHHMQEDIALLAEMGFKTYRFSIS"
     gene            complement(496..732)
                     /locus_tag="PLT73_07365"
     CDS             complement(496..732)
                     /locus_tag="PLT73_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM20232.1"
                     /translation="MLRKTGDLKSLGSKIISITNSSTNSLARQSDINLAYHLTPEYVD
                     EEVNVTSQLPAVFLIETLARRNYNYMQQMLNNKL"
     gene            complement(726..1247)
                     /locus_tag="PLT73_07370"
     CDS             complement(726..1247)
                     /locus_tag="PLT73_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM20233.1"
                     /translation="MFNQENVKLLTNTELKVYEYIVQRTQKVIRMSVREIASETHVSP
                     ATVTRTISKLGFENIWECKLHLKEMDNRKHNKKVFDTSEIVEEFFSRSMQSEYEEKIQ
                     DAVKMIANSDVALFFGIGTSGLIAAYASRQFSNLGQSTFHIQDPYYPFHLISRQYTNT
                     VAFAPSLEKQKSC"
     gene            complement(1419..>2268)
                     /locus_tag="PLT73_07375"
     CDS             complement(1419..>2268)
                     /locus_tag="PLT73_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20234.1"
                     /translation="ILYLDLLESEFAEADGNGEYTEKLKNIRLEFVDRNKLEEIDYNR
                     IRTTDVKFRGKKAWPELLDFTPGLVPWSATEEDITLFERVLESFLEMFPIYSKLDFDK
                     SFAADDVPTRYYYQAGTKDSVWKLKEEHILAFLTGGELTKGPYDLMLSQFEIRRLMME
                     KKTFLRNTFEIDLFYLPQPITFKEGERPRFVKMMAIADKKSGEVLLATVLASDKSRDI
                     QFELYKYLFEAKVFPAKIEMRGDSVLETYEMLVPLLDELDIVHAEKSRLRKIEAFRKS
                     LSEDIF"
BASE COUNT          613 a          511 c          442 g          702 t
ORIGIN      
        1 atgaaatcga gaagcgatag gttttgaaac ccatctcagc caacaaagca atatcttcct
       61 gcatgtggtg gtaatggtca gtggctacct taaaatcgga agtatcggga acgatttcct
      121 tcacatcctg aaccgatggg cctttaccat cttcgtccca agcgccttca acttgatagg
      181 cactagttgc ggatccccat aaaaaacctt ctttaaacgg cttcaatttc ttatgataca
      241 tactctcatc ccttccaatc gctttacaat gggcaagccc acatatttta agcggttaca
      301 tccacttgga aaacaaatgc tctttcctta aaaagagcgg ttttacagtt gcggatgcgc
      361 tttctgtttt tcaatataag cttattatag ggtttgtgaa cgaaatgtaa acagtttcat
      421 ctcgtaaagg aacaatgacc agcttttgga atgttgtttc ataaaagaaa cgatttgttt
      481 caaacgaaaa atctgctaaa gcttgttgtt aagcatttgt tgcatgtaat tataattgcg
      541 tcttgccagt gtttcaatca aaaaaacggc cggtaactgc gaagtcacgt ttacttcttc
      601 gtcgacgtat tcaggtgtca aatgataggc caggtttata tctgactgtc tagccaaaga
      661 attggtagag gaattcgtga tgcttatgat tttgcttcct aaactcttca gatctccagt
      721 cttccttaac atgatttctg tttctccaga gacggagcaa atgccactgt atttgtatat
      781 tgcctgctga tcaaatgaaa tggataatat ggatcttgga tatggaaagt actttgacct
      841 agattcgaaa attgccggga tgcatatgca gcgatcaggc cagaagttcc tatgccaaaa
      901 aagagggcaa cgtcggaatt ggctatcatt ttgactgcat cctgtatttt ttcttcgtat
      961 tccgattgca tggaacggct gaaaaactcc tcaacgatct ccgaggtatc gaataccttc
     1021 ttattgtgtt tgcggttgtc catttccttc agatgcaact tgcattccca gatattttca
     1081 aaccctagct tgctgatggt ccgcgtaacc gttgccggcg acacatgagt ctcggaagct
     1141 atctcgcgaa cgctcatacg gatcactttt tgggtacgct gcacgatgta ctcgtatacc
     1201 tttaattctg tattcgtcag taatttcaca ttttcttgat taaacacgta aaaacccctt
     1261 tatccggaac aaaataagct ctgaggcgtg catgagttcg ttttcttgtt aactatttta
     1321 ccatttattt tgaaaaaaac aaaaaacaaa acaattttat gcaactcaaa aattgttttg
     1381 tttcttgtgc tgttattttc agaaaaataa actatttctc agaagatatc ttccgataag
     1441 ctttttcgga aagcctcgat tttccgtaat ctgctctttt cagcgtgtac gatgtccaac
     1501 tcatccagca agggaaccaa catctcgtaa gtttccaaca cgctgtcccc gcgcatttcg
     1561 atttttgcag ggaaaacttt cgcctcaaac agatatttgt acaattcaaa ttggatatcg
     1621 cgcgatttat cggacgccag gacagtcgcc agcagtactt ctcccgattt tttatccgcg
     1681 atggccatca tcttcacaaa gcgcggccgt tccccttctt tgaaggtgat tggttgcggc
     1741 aaataaaata agtctatttc gaaggtattt cttaagaagg ttttcttctc catcatcaaa
     1801 cgacggattt caaactgaga aagcatcaga tcatacggac ctttcgtcaa ttcaccgccg
     1861 gtcaagaacg ccagaatatg ctcttctttc aatttccaga cactgtcttt tgtacctgct
     1921 tggtaatagt agcgcgtcgg aacgtcatct gccgcaaagg acttgtcaaa gtccagtttg
     1981 ctgtagatag ggaacatttc taaaaagctc tccagcacgc gttcgaaaag agtgatgtcc
     2041 tcttcggtag ccgaccacgg aacaagtcct ggcgtgaaat ccagcagctc cggccaagct
     2101 ttttttccgc ggaatttgac atccgttgtg cggatacggt tataatcgat ttcttccagt
     2161 ttgtttcggt cgacgaattc cagtctgatg tttttcaact tctcagtgta ttccccgttg
     2221 ccatcagctt ccgcgaactc cgactccaac aaatccaagt agaggatg
//
LOCUS       DAOMUJ010000304         2257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13626_length_2257_cov_2.332879, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000304 DAOMUJ010000000
VERSION     DAOMUJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_2257_cov_2.332879"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..377
                     /locus_tag="PLT73_07380"
     CDS             195..377
                     /locus_tag="PLT73_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20235.1"
                     /translation="MASHVIDMIMMKNNFGTEAMRGVWSEDNRLQKHLDVEAALALAE
                     RVSPALNNTVNHRKEE"
     gene            382..1548
                     /locus_tag="PLT73_07385"
     CDS             382..1548
                     /locus_tag="PLT73_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016118470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM20236.1"
                     /translation="MTETQTDTQTKTGFYEKLVAYRRELHAHPELSAKEFATTERIRY
                     WLTEEGVTILDFPLETGLIAEIVGALPGPTIALRADIDALPVKEATGLPFASETEGLM
                     HACGHDYHTASMIGAAILLQGKKASLKGTVRFIFQPAEEIAQGAKWVEESGALKEVSA
                     IFGMHNKPDLPVGTIGIREGGLMASADRFELEIHGVGGHAGIPDSTIDPIVIASQIIT
                     GLQTIISRNTSPFHNAVISVTQIHGGNSWNVIPEKVYLEGTVRAFQKEDREKIPELMR
                     RAAEGIASGFGAVADFRWHPYVSIVDNDVRFKAILAETAEEQGYRFVEPKPVAGGEDF
                     AHYQTLIPGFFVFMGVEGTREWHHPEFNLKEEALAVAADYFAALAIKVLDQWKA"
     gene            1536..>2257
                     /locus_tag="PLT73_07390"
     CDS             1536..>2257
                     /locus_tag="PLT73_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRM20237.1"
                     /translation="MEGLTVFTEMLAAEVLKTTPTKNKAALREAKRGILDYLASAFAG
                     REDAGVLKLLHAIEAEGGHPVAALIGQNKRANRRQAALVNGFMGHALDYDDVHSDVRG
                     HPSSVILPALFALAAGRGISGKQFLDAYIIGVEVMARLGQAFGTISYLQGWHNTSLLG
                     GVAATFAGARLLGFTVAETQNAVGIAASQAAGLRVQFGTEMKPLHAGLAAQQAVQSIE
                     LTQQGFLGSAAALDTTSGYLAA"
BASE COUNT          558 a          536 c          657 g          506 t
ORIGIN      
        1 cggaaaaagg tgaggggtgt tcgttttttt ctatcaagcg tgttcggaaa tgcagaatat
       61 gccgagccac tttgaaatgg taagctaaca gaagttgttt ttgttcattt tgaatggaaa
      121 gcaagcaagt gcggacaaaa gaagccacgc gcagtaagga aatacggata tacgttttgg
      181 ggaggaatca gacaatggca tcacatgtga tagatatgat tatgatgaag aataatttcg
      241 gaacggaagc gatgcgagga gtctggtcgg aggacaatcg cctgcagaag catctggatg
      301 tcgaagcggc attggcgctt gctgaacggg ttagcccagc tttgaataat acagtgaacc
      361 atcgaaagga agaatgatac catgactgaa acgcaaacgg acacacaaac caaaacgggt
      421 ttctacgaaa agctggtcgc ttaccggcgc gaactgcatg cgcatccgga attatcggca
      481 aaggaatttg caacgaccga gcgcatccgc tactggctga cggaagaagg agtcactatc
      541 cttgatttcc cgctggagac cggtctgatc gcggaaattg tgggcgcatt gcctggtccg
      601 accatcgcgc tgcgtgcgga catcgacgct ttgccggtca aagaagcgac aggtctgccg
      661 ttcgcttccg aaacggaagg actcatgcat gcctgcggtc atgattatca tacggcttcc
      721 atgatcggag ctgcaattct gcttcagggt aaaaaagcat cgttgaaagg aacggtccga
      781 ttcattttcc aaccagccga agaaatcgcg caaggggcga agtgggtcga agaatccggt
      841 gctctgaagg aggtttccgc gatcttcggg atgcacaaca agccggatct tccggtaggc
      901 acaatcggga tccgtgaggg cgggctgatg gccagcgcgg atcgcttcga actggagatt
      961 cacggcgtcg gcgggcatgc cggcattcct gattccacca tcgatccgat cgtcatcgca
     1021 agccagatca taaccggact acagacgatc atcagccgca acacgagccc attccataat
     1081 gcagtcatca gcgttaccca gatccacggt ggcaactctt ggaacgtcat tcccgagaaa
     1141 gtctatctgg aagggaccgt ccgcgctttt caaaaagaag atcgcgaaaa aataccggaa
     1201 ttgatgcgtc gggctgctga agggatcgca tccggtttcg gagcggttgc cgatttccgc
     1261 tggcaccctt atgtttccat cgtggacaac gatgtccgat tcaaggcaat cttggcagag
     1321 acggctgaag agcaaggcta tcgtttcgtg gagccgaaac cggttgccgg tggagaggat
     1381 tttgcgcatt accaaacact catacccggt ttctttgtgt tcatgggcgt ggaaggcaca
     1441 cgtgagtggc accatcctga gttcaatctc aaggaggaag ccttggcggt cgcagcggat
     1501 tatttcgcag cgttggcgat caaggtgctg gatcaatgga aggcttgaca gtatttactg
     1561 aaatgctggc cgctgaagtt ttgaagacaa cccctacaaa aaataaagct gccttgcgtg
     1621 aagcaaaacg cggtatcctc gactatttgg ccagcgcttt tgctggccgg gaagatgccg
     1681 gtgtgctgaa gttgctacac gccatcgaag cggaaggcgg gcatcctgtc gcggctttga
     1741 tcggccaaaa taaacgagca aacaggcggc aagcggcact tgtgaacggg tttatgggac
     1801 atgcgcttga ttatgacgat gtccattccg acgtgcgcgg tcatcctagc tcggttattt
     1861 tgccagcgct ttttgcgttg gctgccggac ggggaatttc cggcaaacaa tttttggatg
     1921 cctatatcat cggcgtggaa gtgatggcaa ggctcggcca ggcttttggg acgataagtt
     1981 atttgcaagg atggcacaat acctcgttgc tcggtggcgt cgcggctact tttgcgggag
     2041 cccgattgct cgggttcact gttgccgaaa cgcagaatgc tgtcggaatc gccgccagtc
     2101 aggctgctgg tctgcgcgtg cagttcggaa cggaaatgaa gccgttgcat gccggtctgg
     2161 cagcgcaaca agcggtccaa tcaatcgaat tgactcagca aggatttctt ggctctgcag
     2221 cggcgcttga tactacaagc ggctatctgg ctgcata
//
LOCUS       DAOMUJ010000305         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13698_length_2247_cov_2.577099, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000305 DAOMUJ010000000
VERSION     DAOMUJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13698_length_2247_cov_2.577099"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLT73_07395"
     CDS             complement(<1..432)
                     /locus_tag="PLT73_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20238.1"
                     /translation="MERQAYVEKLKLKFPGDAELLFRDLSFSVTAGEKVLFLGPSGCG
                     KSTLLQVLSGLIPQSIEVPMKAEKLVTPASWGYVFQDPDSQFCMPYVDEELAFVLENL
                     SVPREEMAAKIDFALRQVGLELADSHIPIASLSGGMKQRLAI"
     gene            complement(447..1025)
                     /locus_tag="PLT73_07400"
     CDS             complement(447..1025)
                     /locus_tag="PLT73_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM20239.1"
                     /translation="MKKELKLTDILVTIVIAIAFGIVYILWGTVYYAVQPLGLHLDQL
                     LYGMWFIAGTVAYFIIRKPGVALLAEIAAASGEFLLGSPWGLTVLLSGVVQGLFAELV
                     FMAFRYKRFDLKVAALAASASCVGSLLVDALNGQIAELAPWNLALYLLARFAGSIFFA
                     GVLAYYLAEVLEDTGVTNLVRPLSTEDFKDMD"
     gene            complement(1061..1735)
                     /gene="tenA"
                     /locus_tag="PLT73_07405"
     CDS             complement(1061..1735)
                     /gene="tenA"
                     /locus_tag="PLT73_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HRM20240.1"
                     /translation="MTFSAQIRQAVEPIWAASMKHPFVKGIGDGSLPLENFRFYIQQD
                     SYYLTRFAKILALGAAKAPDIATTNQFADFAKHTYSAEIGLHEQFSKRLGITAEEKEL
                     FKPAPTAYAYTSHLYHAGHSGHLGDVIAAVLPCYWLYAEIGDAFIDAAPEEPIYQEWI
                     ATYGGKDFQDRVDEQIEHLDKIAAAVSEADRERMKEHFIISSQYEYAFWEMAYRMETW
                     PVPLEK"
     regulatory      complement(1883..2009)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          539 a          649 c          538 g          521 t
ORIGIN      
        1 gatcgccagc cgctgcttca tcccaccgga aaggcttgcg atggggatat ggctgtccgc
       61 caattccaac cccacttgcc ggagtgcgaa gtctattttg gcagccatct cttcgcgcgg
      121 gaccgacaga ttttcaagca caaaagcgag ttcttcatcg acatagggca tgcagaattg
      181 gctgtccggg tcctggaaaa catagcccca ggaagccggc gtcacgagct tttcggcctt
      241 catcggcact tcgatcgatt gcggaatcag cccgctcaac acctgcagca gcgtggattt
      301 gccgcagccg gatggcccca aaaacagcac tttctcgccg gcagtgaccg aaaaggatag
      361 gtccctgaac aacaactcgg catcgccggg aaatttcagt ttcagtttct ccacatacgc
      421 ttgccgctcc atttccgcca tcctccttaa tccatatctt tgaaatcttc agtcgaaagc
      481 ggacggacaa ggttcgtcac gccggtatcc tccaggactt ccgccaagta ataggccagc
      541 acaccggcaa agaaaatcga gccggcgaat cgggcaagca ggtaaagcgc caagttccaa
      601 ggagccagct ccgcgatctg cccgttcagc gcatccacca aaagcgaacc cacacaagac
      661 gccgaagctg ccaaagcggc aaccttcagg tcaaaacgtt tgtagcggaa agccatgaac
      721 accaattccg caaacaaccc ctgcaccacg ccggaaagca agacggtcaa gccccacggc
      781 gatcccaaaa ggaattcgcc tgaagccgca gcgatttccg ccaacagcgc cacacccggt
      841 ttgcggatga tgaaataggc gacggtaccg gcgatgaacc acatgccgta cagcagttga
      901 tcaagatgca gacccaacgg ctgcaccgcg taatagactg ttccccataa aatgtagacg
      961 atcccgaaag cgatggcgat gacgatcgtc accaaaatat ctgtaagctt caattctttt
     1021 ttcattttca ctgtctcctc tttttgttta attttgtcgc ttatttctcc aacggtaccg
     1081 gccaagtttc catgcgatag gccatttccc aaaacgcgta ttcgtattgg ctgctgatga
     1141 tgaaatgctc tttcatcctt tcgcggtcgg cttcgctcac ggctgcggca atcttatcca
     1201 gatgctcaat ctgctcatcc acccggtcct gaaaatcctt cccgccatag gtagcgatcc
     1261 attcctgata gatcggttct tccggtgctg catcaatgaa ggcatccccg atttcggcgt
     1321 agagccagta gcaaggcaga acggcggcga tcacatcccc caaatgcccc gagtggcctg
     1381 catgatagag atgggacgtg taggcgtaag cggtcggtgc gggcttgaaa agctcttttt
     1441 cctccgcggt gatgcccagc cttttcgaaa actgctcatg caggccgatt tcggcgctgt
     1501 acgtatgctt cgcgaagtcg gcgaactggt tggtcgtcgc gatgtccggt gctttggccg
     1561 cgccgagtgc caaaattttg gcgaagcgcg tcaggtaata ggaatcctgc tggatgtaga
     1621 aacggaaatt ctccaacggc aagctgccgt ccccgatccc tttcacgaac ggatgcttca
     1681 tgctggctgc ccagatcggt tcgaccgctt ggcggatttg tgctgaaaat gtcattgctg
     1741 tccctccata tgctgtgaac gctccgccag ggtcccgact tatccggacg gccgctcttg
     1801 atttttgcaa acaaaaaaac cactctcttc ccaacggaaa aaagtggttg tatgttcctt
     1861 atgcaatagg gacttactta gccaaaagag gccgccactt ccctacgctg gtacaaacca
     1921 gatcaggttc taagggtctc gaagtcctac ttcgatctca gctttttggc ggacagtcat
     1981 ccgccggtaa agcaccccta gtggtaaaaa gaatattcag ttcatttgct gttgttctaa
     2041 tcgtatcata atttttccgg aagtcaactc gttctgttgc acgaaacgtt accgtttctt
     2101 gtatttattc accatatgca gccaacaacg cttgtccgtc tgcttcaaaa acctgcaatg
     2161 cctgcagcgc caaatccttg ttcatcatgt tattcaggac atccggaggg atcgtggcag
     2221 tatgcgcgcc cgactccaaa gaatcga
//
LOCUS       DAOMUJ010000306         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13700_length_2247_cov_1.997263, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000306 DAOMUJ010000000
VERSION     DAOMUJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13700_length_2247_cov_1.997263"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..2012
                     /locus_tag="PLT73_07410"
     CDS             411..2012
                     /locus_tag="PLT73_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRM20241.1"
                     /translation="MLFFRKRSKTPDPQEGKMSNPWEKEKKSYQSTIEKLVARKADIY
                     AHLGAAIAYQAKDDPAVLLPYPIQAAALNDIEQKTEAFRKRMAQAESIIQHEEVPWTE
                     ICSCGARCPQSARFCCVCGAPIHRSDTNKTADDVSSLKSAGNRALTGLQSLPAIPATR
                     AAMESSSHFAYESAIHILASESNICYSPVSLEMALLIAMQGAGGNTLAQLQRVLACTA
                     LSEQDLASIYRSIVGKRKGKSQFDAANSLWIPQTFQVNRGYTHTTKRLYDTQVKQVEG
                     FNEKTNTAINNWIKKETRSLLNPELVIPSNTKLVIINTLYADGRWSSPFEDTEQAEFH
                     SLRGQTTIIDFMDKTFDIDGHADDPVAYVKGDGWQRADIAFDNGGCMKILLPDDNARF
                     KRLRAKASKLRDAFNASPTVQNDTKIHVQLPKFDIDSFFDKEMIQTLQAMGITDAFNQ
                     RKADFSTLSDTPLLISKILQGTHIEVAEEGAKAAAYTVIMMEPPICPAPKPVKWIDFI
                     VDHPFLFELDSPDGLPLFIGAVTEL"
BASE COUNT          614 a          619 c          540 g          474 t
ORIGIN      
        1 cgctggtcat catcgtcatc accggcatac tcatgtgcct cgtgaatctc agcgagcgta
       61 tgctgctcgg acccgtaggc gaggacgagg atcctgatgc cgggcagtga gcaggccgct
      121 ctcctccctc tacccatcgt gcgtgacgcg atccgcccgc gcagcttgcc gttcgattcc
      181 gacgattcgt cacttggctg tcgattcgga cgaaatcagg gtcgatttcg tccgaatcgg
      241 cagccatgtg caccacaaca gacaaacgaa ccaccaagtt cgccatgcga cgtttaccct
      301 gacttttctg acgtttacat gaactcgtcc ggcaataccg cgctttctta caagaatgga
      361 atgcacaaga gcaaacgctg ctatacgcca ggattccgga gaaaaccgac atgttatttt
      421 ttcgcaaacg cagtaaaacg ccagacccac aggaagggaa aatgtcaaac ccgtgggaga
      481 aggaaaagaa gtcctaccaa tccacaatcg aaaaacttgt tgcacgtaaa gccgacatat
      541 acgcccacct tggcgctgcg atcgcatatc aagcaaagga cgatccagca gtcttgctcc
      601 cctatccgat acaagctgcc gcgctgaatg acatcgagca aaaaacagaa gcgttcagga
      661 aacgaatggc acaggcggaa agcatcattc agcacgagga agttccctgg acagaaatct
      721 gttcgtgcgg ggcacgctgc ccgcaatcgg cgagattctg ttgtgtatgc ggtgcgccaa
      781 tacatcgaag tgacacaaat aagacagccg acgacgtttc gagcttgaaa agcgctggca
      841 atcgcgcatt gaccggcctg caaagtctgc ctgcaatacc ggcaacccga gcagcaatgg
      901 aatcatcgag tcactttgct tatgaaagcg ccatacacat ccttgcttcc gaatccaata
      961 tctgctattc ccccgtatca ctcgagatgg cgttgctgat cgcgatgcaa ggcgcaggag
     1021 ggaacacact cgcccaattg caaagagtgt tggcatgcac agcactcagc gagcaggacc
     1081 tcgcttccat ctaccgatcg atagtcggca agcgtaaagg caaatcacaa ttcgatgccg
     1141 cgaattccct atggattcca cagaccttcc aagtcaatcg cggctatacc catacgacga
     1201 aacggctata cgacacacag gtcaagcaag tcgaaggctt caatgagaaa acaaacacag
     1261 ccataaacaa ctggattaaa aaagagacgc gaagcctgct caatccggaa ctcgtcattc
     1321 cttccaacac caaattggtg attatcaaca ccctctatgc cgatggccgc tggagttctc
     1381 cttttgaaga caccgagcaa gccgaatttc attcgttgag gggacagaca accatcatcg
     1441 atttcatgga taagacgttc gatatcgatg gccatgcaga cgatcccgtt gcctatgtca
     1501 agggtgacgg ttggcaacga gcggatatcg cattcgacaa tggcgggtgc atgaaaatcc
     1561 tgctgcccga cgacaatgcg cgattcaaaa ggcttcgcgc caaggcttcc aagctacggg
     1621 atgcgttcaa cgcttcccca accgtacaga acgacaccaa gatccatgtg caactaccga
     1681 agttcgacat tgacagtttc ttcgataaag aaatgattca aacgttgcag gcaatgggga
     1741 ttacggacgc tttcaatcaa agaaaagcag atttttccac actttcagat acacctctgc
     1801 tcatcagcaa aattctacag ggaacgcata tcgaggtggc ggaggaaggc gcgaaggccg
     1861 ccgcatatac agtgataatg atggagccgc caatctgtcc cgcgccaaaa cctgttaaat
     1921 ggatcgactt catcgtcgat catccgttcc tcttcgaact tgactccccg gatgggttgc
     1981 cgttgttcat tggcgcggtg accgagctat gaacaacgtg tggtggggtt cgacgatgat
     2041 tcgccgaacc ccaccacatg ttgtcatgcc gtcagcatcg ataattcatt tcactccgtg
     2101 taaaaggcat tcatgccgga gcgaatatac gattcaccgg cacgaagcac gaatcctgcc
     2161 gttgatgtat tgtcccatct gcttagtggt ttcaacatca gggcaataca ttgtctctcg
     2221 gcgactggca agctgcaata taatggc
//
LOCUS       DAOMUJ010000307         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13844_length_2230_cov_1.781609, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000307 DAOMUJ010000000
VERSION     DAOMUJ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13844_length_2230_cov_1.781609"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLT73_07415"
     CDS             complement(<1..231)
                     /locus_tag="PLT73_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20242.1"
                     /translation="MIQAQITGLKKSFGKNVILKNVGMTINKGEVVCIIGPSGSGKTT
                     FLRCLNLLERPDAGMYELGDLRYDLSRIGSHQS"
     gene            complement(258..>639)
                     /locus_tag="PLT73_07420"
     CDS             complement(258..>639)
                     /locus_tag="PLT73_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM20243.1"
                     /translation="LSIHIPLSDKSTHLFSEDTFEKMKPGSCLVNTARGKIVDTEALI
                     QALATGKLAGAALDALEDEEKYFSSGWEKNPYYARLMAFPNVLITPHIAYHTELAVQE
                     IVETSLNNARDIITAGSSRNTISL"
     assembly_gap    640..649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<650..1224)
                     /locus_tag="PLT73_07425"
     CDS             complement(<650..1224)
                     /locus_tag="PLT73_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM20244.1"
                     /translation="MRLIYYGVSADEEKFIKRWSFSNKIPVTIINEGISSENVHFAGP
                     HDGVCLYPSPEMRQSELLYRKLREQGIHQLSIKSTGVDGVNFEWAQKYGLTVTNVPSY
                     SPTSVGHFAIMSILMVLRNIPTVLQTNPSRKTTIGRELQDVTVGILGTGRIGSVVAEG
                     IHALGGRVIASSSKENPRLKGKVSYVSFEEL"
     gene            complement(1236..>2230)
                     /locus_tag="PLT73_07430"
     CDS             complement(1236..>2230)
                     /locus_tag="PLT73_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM20245.1"
                     /translation="RKLVKERLAATANLHNEEQVLITSGSTQAMDIAVKVLCDEGDVV
                     LCEEQTFSGAVNAIKGYGAIPVPIPMNVSEESVDLEALEELLRFDRRIKFIYLIPTFQ
                     NPLGTSIPLEKRQAIYSLARKYDVLIFEDDPYGDLLYYGDPIMKLKEMDTDGRVMYAG
                     SFSKILAPSARLGFIMAPDAIMEKLALAKQVSDSHTNFYWQVMLAEFMQNHQFEEHVS
                     ELKLYYKGKFEAMIAGLEALPEDKIQFIKPTGGYFICCKMSDELDPETFYTALDEAKV
                     AVIPGNIMSVAQEGYDRYFRLNFTKPTLSEIVAGVKVIGEALEQATPASYKQVI"
BASE COUNT          524 a          571 c          507 g          618 t
ORIGIN      
        1 gctctgatgc gaaccgatac gactgaggtc atatcgaagg tcgccgagtt catacatgcc
       61 tgcatccggc ctttccaaga gattcaaaca acgcaagaag gtggtttttc ccgatccgct
      121 cggtccgata atgcagacaa cttcgccttt attgatggtc atacccacgt ttttcaatat
      181 tacgttcttt ccgaaacttt ttttcagtcc tgttatttgc gcctggatca tcgtcatctc
      241 ctccttcctt ttggttctca aagtgaaatc gtgttgcgcg aacttcctgc tgttatgata
      301 tcccgcgcat tgttcaacga ggtctcaaca atttcttgca cggccaattc tgtatggtag
      361 gcaatatgcg gtgtgatcag cacattcggg aaggccatca agcgggcata atacgggttc
      421 ttttcccaac cgctggaaaa atatttctcc tcgtcctcca acgcatccaa ggccgcaccc
      481 gccagttttc cggttgccaa agcttggata agcgcttcgg tgtcgacgat ttttccgcgt
      541 gctgtgttca caagacaact accaggtttc attttttcaa aagtatcttc agaaaaaaga
      601 tgcgtggact tatcggataa aggaatgtgg atagaaaggn nnnnnnnnna acagttcttc
      661 aaaagaaaca tagctgactt tccctttcaa gcgcggattt tcttttgagc ttgaggcaat
      721 cacacgaccg cccaaagcat ggatcccttc cgcgaccaca gaaccgatgc ggcctgtccc
      781 caggatgccg accgttacat cctgcagttc ccgaccgatt gttgttttgc gggatggatt
      841 tgtttgcagc acggtcggga tattccgcag caccatcaaa atggacatga tcgcaaaatg
      901 accgactgaa gtggggctgt aggaaggaac gttcgtcacg gtcaaaccgt atttctgcgc
      961 ccattcgaag ttgacgccat ccacccctgt ggatttgatc gacaattggt ggatgccttg
     1021 ctcccgcagc ttgcggtaaa gcagctcgct ttggcgcatt tccggactcg gatacaggca
     1081 gaccccatcg tgggggccgg cgaaatggac attttcgctg gatatgcctt cgttaatgat
     1141 ggtcacgggt attttgttgc tgaaactcca gcgtttgatg aatttttctt cgtcagcgct
     1201 gactccataa taaatcagtc tcatatttga cctcctcaaa tcacttgttt gtacgatgcg
     1261 ggtgtcgctt gttccaaagc ttcaccgatt actttgacac cggccacgat ttccgatagt
     1321 gtcggtttgg tgaaattcaa gcggaaataa cggtcgtagc cttcctgcgc cacgctcatt
     1381 atatttcccg ggataacggc gactttggct tcgtccaatg ccgtatagaa tgtctccgga
     1441 tccaattcgt ccgacatttt gcagcagata aaatagccgc cggtcggttt gatgaattga
     1501 attttgtctt caggcaatgc ttccagaccg gcaatcattg cttcaaattt ccccttataa
     1561 tacagcttca actccgatac atgttcttca aactgatgat tctgcatgaa ttccgctaac
     1621 atcacttgcc agtaaaaatt ggtgtgggaa tcggagactt gcttggccaa agccagtttt
     1681 tccatgattg catccggcgc cataatgaag cctaagcgtg cgctcggtgc caaaattttg
     1741 gaaaacgaac ccgcgtacat gacgcgccca tccgtatcca tttccttcag cttcatgatc
     1801 ggatcgccgt agtacaggag atctccgtaa gggtcatctt cgaatatcag cacatcatat
     1861 tttcgggcca agctgtagat cgcttggcgt ttttccagag ggatcgatgt ccccaatgga
     1921 ttttggaacg tgggaatgag gtagatgaac ttgatgcgcc gatcaaaacg caacagctct
     1981 tccagtgctt ccaaatcgac ggattcttct gagacgttca tcgggatcgg tacaggaatg
     2041 gctccgtagc ctttgatcgc attcaccgcc cctgaaaagg tttgttcttc gcacaacacg
     2101 acgtctccct catcacacag aactttcacg gcgatatcca tcgcttgagt ggaacccgat
     2161 gtaatcaaca cctgttcttc gttatgaaga ttagcggttg ctgccaaccg ttctttgacc
     2221 agcttacgca
//
LOCUS       DAOMUJ010000308         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_13880_length_2226_cov_2.600645, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000308 DAOMUJ010000000
VERSION     DAOMUJ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13880_length_2226_cov_2.600645"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>244)
                     /locus_tag="PLT73_07435"
     CDS             complement(<1..>244)
                     /locus_tag="PLT73_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20246.1"
                     /translation="KAEQLPYPGEMKNLIRSAIAFSKPNDENDYLRRIYIALIPNGAN
                     IIQDIRMLRDQGFTLREIEVLTGISRSTAAREMKGTH"
     assembly_gap    245..259
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<260..1069)
                     /locus_tag="PLT73_07440"
     CDS             complement(<260..1069)
                     /locus_tag="PLT73_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM20247.1"
                     /translation="MKKKNVLNLIRYHTEHNDAAFRNEAYNIAKEFDHAGDSQLAAYI
                     IALLSDANTLVPQSSNSDIKSEFLEKKIVVKASLPLPEVIADDIQGALNAIGRNIGIH
                     KFLFHGPAGTGKTESVKQISSIMQRELYVVNFSSVIDSRLGQTAKNITKLFAEINSFT
                     RPDQTIVLFDEIDALALDRIDSHDIREMGRATSTLLSQMDELSEQIILFATTNLFSKF
                     DKAFIRRFDAVVDFGRYTKEDLIDVATSIMNDYAKQFSFIGKNIRLFKKILS"
     gene            1342..1521
                     /locus_tag="PLT73_07445"
     CDS             1342..1521
                     /locus_tag="PLT73_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HRM20248.1"
                     /translation="MAANFKPGEDNHRPGTYIEVGPRGGRVHDPRIVHIDAGDRLPPT
                     QEVGHLWQHRPSGKK"
BASE COUNT          538 a          446 c          585 g          642 t
ORIGIN      
        1 tgtgggtacc tttcatttct cgagctgctg tgctgcgtga tattccagtt agaacctcga
       61 tttcgcggag tgtgaatcct tgatctcgca gcattcgaat gtcttgaata atatttgctc
      121 cattggggat aagagcgatg taaatgcggc ggagataatc gttttcgtca ttgggcttgc
      181 tgaatgcgat ggccgatcgg atgagatttt tcatctcgcc cgggtagggt agctgctctg
      241 ccttnnnnnn nnnnnnnnna gaaagaatct tcttgaagag acgaatgttc tttccgatga
      301 acgagaattg ctttgcatag tcattcatga tgcttgttgc cacatcaatg agatcttctt
      361 tcgtgtacct tccaaaatca actacagcat caaatctgcg aatgaatgct ttgtcgaatt
      421 ttgaaaatag gttggtggtc gcaaacaaaa taatttgctc tgagagttca tccatttggc
      481 tcagcaaggt cgatgtggca cggcccattt ctcggatgtc atgtgaatca atacggtcaa
      541 gagcgagcgc atcgatttca tcaaaaagta ctatcgtctg atcagggcgt gtgaaactat
      601 tgatttcagc gaatagtttg gtgatgttct ttgccgtttg accaagacgg ctgtctatga
      661 cagagctgaa attgacgaca tacaattcgc gttgcatgat tgaggaaatt tgttttacgg
      721 actcagtctt tcccgtgccg gcagggccgt gaaaaaggaa tttatggatt ccgatatttc
      781 gcccgatggc gttgagtgct ccctgaatgt catctgcaat gacttcaggt agtggcagag
      841 aagccttgac cacgattttc ttttcgagga attcggactt gatgtcggaa ttagacgatt
      901 gcgggacaag agtgtttgcg tctgatagta aagcgatgat gtacgcggcc agctgtgagt
      961 ctccggcgtg atcaaattcc ttggcgatat tgtacgcttc attgcggaaa gcggcgtcat
     1021 tatgctccgt atggtaccgg attagattca gcacattttt cttcttcatg tttcttagcc
     1081 tttaagtcgt aaattgcaac tttaagtaat ttattctgtg tctcattgta gcatatgtgt
     1141 gtctcagtcg ttttggtggt atgctgtgta tgcagtctca aatgggctgc gggaaaataa
     1201 aaactccgat tcgttggcgc gaatcggagt cgggttgagc cctagggctt gtgtgtctcg
     1261 gtaagacctc acaagactac cgcaatggct tgtcaatgca ggtcgggcga ctgggctcct
     1321 tagttcagga aggagctgtc aatggcagca aatttcaagc caggtgaaga taaccaccga
     1381 ccgggaacat atatagaagt tggaccgcga ggaggaaggg tgcatgatcc tcgcatcgta
     1441 catattgatg caggtgacag gcttccgcct acgcaagagg tcggacatct ttggcagcat
     1501 cggccttctg gcaagaaata gaagagaagg gaagacgggg tgcttcgatt gctggattga
     1561 agatattctg aattccagca atcgaagcgt gactcctctt cggaagtatt gtcagtgctt
     1621 gattttgtgg aagagaaagg ttttttgagt agtgatgtga ttttgttact gggaacagtg
     1681 actcacgata aacatctgct gctgtgtaat ggccgttttc ttagggaaga aaggtcgggg
     1741 gagtgtctgt cgacgcacaa gtgggatgtg ggtttgtaag gcagtttgct tttagtctgt
     1801 ttgcttcagg aatccaattc acggagatac cgcggatttg tctgatattc gaccatgcgt
     1861 ttatcgaatg tgcgctggat aggggttgtc tgcctgtctt cggactgaag cattcggatc
     1921 cgcagaatct gattgataat tcaatcgccc gtacgaccgg caccttgagg gcataaatca
     1981 tgcttgggtc caaccagaag aaccgacaaa actgattctt ggcggattct ggctgtcttt
     2041 actgcactta caacagcttg cataaccaaa aatccgcgag acccctaaac gtcagtcgcg
     2101 cggatttctg ctatcaatgg ttattcatcg tttttaacga tcttgagcga cggatgaaac
     2161 ggtcgtcagt agataacgaa tgattgtgca ctcggatgga gactttgtcc cacagcagct
     2221 cgaacc
//
LOCUS       DAOMUJ010000309         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14059_length_2206_cov_1.832636, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000309 DAOMUJ010000000
VERSION     DAOMUJ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14059_length_2206_cov_1.832636"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1295)
                     /locus_tag="PLT73_07450"
     CDS             complement(291..1295)
                     /locus_tag="PLT73_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20249.1"
                     /translation="MNQDERKKFWDVLLTMHDAAPEHIQQLQKTIIIPNTLCRFRAVN
                     ENSLVQLQENKLYFSTSDYYDDPFDTYFYLDYDAIDRQIESIVSLLNNPENKEKATKI
                     VTSLGIPSEDVLRSLDAATQNPITAEAFEESIKNVRSQILKTLYSICFCEDPLNETLW
                     LKYADSHRGFVVTYDFHSADTYLCGKDGICKNCPTIQTPPNIYPVYYSNEKYDASRYA
                     IAALLKVSMASSGVSIPQGLLRAADASMIWEIERISLLKKPCHVFDEEWRMIYSQWCV
                     ARPCIKLKPKSVIIGYRTPLYKERLIISAATVAGIKDVRKIQINRKDELEAVPIQIKN
                     "
     gene            complement(1335..1763)
                     /locus_tag="PLT73_07455"
     CDS             complement(1335..1763)
                     /locus_tag="PLT73_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRM20250.1"
                     /translation="MDDLTPEQRKKNMRAIRSKDTTIELALRKALWQRGIRYRKNYKG
                     LIGKPDIVITKYRIAVFCDSDFWHGYDWENRKSRIKSNQNYWIPKIERNMKRDREVTA
                     ALVEQGWIVLRFWEHTIRKELEECVNDIQEAVDVRKELFK"
     gene            complement(1886..>2206)
                     /locus_tag="PLT73_07460"
     CDS             complement(1886..>2206)
                     /locus_tag="PLT73_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20251.1"
                     /translation="SGGTNYYPFYPSNDTIEQLDKLLAPFTEDEPVYQVSLRIMKEIL
                     SHIESDDDFRVTAFISILEMMLSEKPNAQGLSAEIEMLLKELAHCCRRMIGHWAASIQ
                     HRLC"
BASE COUNT          614 a          489 c          421 g          682 t
ORIGIN      
        1 gccggactac aatgcgtggg aaattgttaa gcgtaccaat gcctgcctga tggaagaccc
       61 ctattggcta cgatttagcg aggatgaaac tttcgaggac accacgcgcg gacgagccag
      121 aaaaatcatg gacgaaactc agaataacag ctgatactaa ttggtatcag ttgacaagta
      181 tagcaaaacc cacaggttgg acacacatac cagcctgtgg gctttttatt cgcagagtaa
      241 aataaattga accaaatttg aaatatcaaa aatcacttca gctcaatatt tcaatttttt
      301 atttgaattg gaaccgcttc caattcatct tttcggttaa tttggatttt acgaacatcc
      361 tttattcctg caactgttgc ggccgaaata ataaggcgtt ctttgtatag gggtgtacgg
      421 tatccgatga tgacactttt gggctttagt ttaatgcaag gccgtgcaac acaccattga
      481 gaataaatca tacgccactc ctcatcaaaa acgtggcacg gtttctttag gagagaaatt
      541 ctttcaattt cccatatcat agaagcatca gctgccctta acagcccttg cggaatagaa
      601 accccgctag atgccatact gacttttagc aaagcagcga ttgcatatcg agacgcatca
      661 tatttctcgt tcgagtaata taccggataa atattgggcg gggtttgaat ggtcggacaa
      721 tttttgcaaa taccatcttt cccgcataaa taagtatctg cgctatgaaa atcatatgta
      781 acaacaaatc ctcggtgact atccgcatac ttcagccaga gggtttcatt tagcgggtct
      841 tcgcagaagc aaatggagta aagtgttttt agaatttggc tgcgaacatt ctttatgctc
      901 tcttcaaaag cttctgccgt tatcggattt tgggtagccg catccaatga ccgcaagaca
      961 tcttctgacg gaattcctaa agacgtaaca atttttgttg ccttttcctt attttcaggg
     1021 ttgttcaata gagaaacaat actttcgatt tgacgatcaa tcgcgtcata gtcaagataa
     1081 aaatatgtat cgaatggatc atcatagtaa tcagaagttg aaaagtacag cttattttct
     1141 tggagttgta ctaaactatt ctcattgacc gcacggaatc tacacaacgt gttcggaata
     1201 ataatagttt tctgtaactg ctgaatatgt tccggtgcgg catcgtgcat agtcagcagt
     1261 acatcccaga atttttttcg ttcgtcttga ttcatagcgg cctcctctca tccatgcaac
     1321 ctagcaattt ttcattattt gaaaagttct tttctcacat caactgcttc ttgaatgtca
     1381 ttgacacatt cttccaactc tttccgaata gtatgctccc aaaagcgcag aacaatccag
     1441 ccttgctcaa caagagctgc tgtaacttct cgatctcgct tcatgtttct ctcaattttg
     1501 ggtatccagt agttttgatt cgacttgatt cgagattttc tgttttccca gtcatagcca
     1561 tgccaaaaat cggaatcgca aaacacggca attcgatatt ttgtgattac tatatccggt
     1621 tttcctatta agcctttata gttttttcgg tatcgtatgc ctctctgcca tagagctttc
     1681 cgcagcgcaa gttctattgt tgtatccttg cttcggattg ctctcatatt tttctttctc
     1741 tgttcaggtg ttaaatcatc cataaatcag atcgaagaat cgtcctcttg aacgtcataa
     1801 taaatcaaac cgttcggtag cttaatgttg ggaacccaca gttcctgtcc gccccagcct
     1861 tttttgccag taaccttata cattgtcagc acagtcgatg ctgtatactg gccgcccaat
     1921 gcccaatcat tcggcgacag caatgcgcca gttccttgag taacatctct atttctgcgg
     1981 acaatccctg cgcatttggc ttttcagaca gcatcatctc aagaatagag ataaaagctg
     2041 tcactctaaa atcgtcatcg gattcaatgt gggaaagaat ctccttcatt atgcgaaggc
     2101 tgacctgata aacaggttca tcttctgtaa agggagccag cagcttatca agttgttcaa
     2161 tcgtgtcatt gctgggataa aatggataat agttggttcc accaga
//
LOCUS       DAOMUJ010000310         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14112_length_2200_cov_1.581352, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000310 DAOMUJ010000000
VERSION     DAOMUJ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14112_length_2200_cov_1.581352"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLT73_07465"
     CDS             complement(<1..404)
                     /locus_tag="PLT73_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRM20252.1"
                     /translation="MEMEKMTTALQQAIAEAQRIAHLWKIFLQPDSFARNFYIDSGVA
                     IDAFESAVDKELDKSVVIEGTSVQYGQTFSQNLYNLFLEADRKRSELKDDFIATDTVL
                     LSLMTLKNHPLTKFLVNLGLTEKIIAEKIKDM"
     gene            complement(535..879)
                     /locus_tag="PLT73_07470"
     CDS             complement(535..879)
                     /locus_tag="PLT73_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRM20253.1"
                     /translation="MTEETTPQFSQEELESIKERVLAALEQVIDPELGIDIVNLGLIY
                     EVELEQDGFCLIKMTLTTMGCPLADVITDEIHRALKDVPEVTETQVKLVWYPAWTTDR
                     MSRYARIALGIR"
     gene            complement(938..1702)
                     /locus_tag="PLT73_07475"
     CDS             complement(938..1702)
                     /locus_tag="PLT73_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM20254.1"
                     /translation="MILIERKIINDIPILEIVEEDKKQETTPLVFFYHGITNQKERGL
                     EPGYALASNGMRVIIPDAYRHGERKEEAYAGEPAAEFWSVVLKNIKELPDIVEAYVEA
                     GLAKRETVSVTGLSMGGITTCIALTQYPWIHSAACLMGSPDPIGLSHWALQSRWAEGL
                     PPLDDEKVNALMAPFENLSLKEHPESLVDTPFYIWHGTADESVPYEQMEQFVNSISGE
                     AYADNICFRSTEGAGHRVPYAIFEEMAVFLGNVYSH"
     gene            complement(1735..>2200)
                     /locus_tag="PLT73_07480"
     CDS             complement(1735..>2200)
                     /locus_tag="PLT73_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM20255.1"
                     /translation="FASTATLPSNEYFPKNLLATQITSKDKLSENPTSMNVFSEERNL
                     HAIRDRILARYGNSIEIRTWGGTMPCLEIVSAGVQKALGVEVVANNYGIKREDILAFG
                     DEDNDMEMIRFAGHGVVMQNGIGALKRIADDVTTHTNHEDGLAIYLEKYFCL"
BASE COUNT          553 a          563 c          454 g          630 t
ORIGIN      
        1 ctcatatcct tgattttttc cgcgatgatt ttttccgtca ggcctagatt aaccaaaaat
       61 ttggtcaatg gatgattttt cagtgtcatc aagctcaaaa gcaccgtatc agtggcgatg
      121 aaatcatcct tcaactccga ccgcttgcgg tctgcttcca gaaagagatt gtacagattc
      181 tggctgaatg tttgcccgta ctggacggaa gtgccctcaa tcaccactga tttatccagt
      241 tccttatcaa cggctgattc aaatgcatcg attgccactc ctgaatcgat ataaaaattg
      301 cgcgcaaaac tatccggctg cagaaatatt ttccacagat gggcgatacg ttgcgcttct
      361 gcgattgcct gctgcagggc tgtcgtcatt ttttccattt ccatgctagg acctcctcaa
      421 aaaatctatt gatatatctt ttataggaca agtatacgac catggtcaga attggtcaaa
      481 tgataacccg aaaaaaaaga acaacaaagg tgatttcctt tgttgctctt cttatcatcg
      541 gatccctaac gcgatgcgtg catatcggga cattctgtct gtcgtccagg caggatacca
      601 caccaatttc acttgtgttt cggttacttc aggaacgtct ttcaaagcac gatgaatttc
      661 atcggtgatg acatccgcca atgggcagcc catcgttgtg agtgtcatct tgatcaggca
      721 gaatccatcc tgttccagtt ccacttcata aatcaaacct aaattgacga tatctattcc
      781 taattccgga tctatcactt gctcaagcgc cgctaagacg cgttctttta tgctttccaa
      841 ttcttcttgc gaaaattggg gagttgtctc ttcagtcatt gcaatcacct cttacctttc
      901 tggattaatt agataataac agtttgtgca tagaaattca atgcgaatat acgttaccta
      961 aaaatactgc catttcctca aaaatagcgt aaggaacccg gtgtccggca ccttctgtcg
     1021 accggaaaca aatgttatct gcataggctt ctccgctgat ggagttgaca aactgctcca
     1081 tctgctcgta cggcacactc tcgtcggcgg taccgtgcca gatgtaaaac ggcgtgtcaa
     1141 cgagcgattc cggatgttct ttgaggctca ggttttcaaa aggcgccatc aacgcattga
     1201 ccttctcgtc atcgagcgga ggcagacctt ccgcccagcg ggactgcagt gcccaatggg
     1261 acaacccgat cggatccgga ctgcccatca agcaggctgc cgaatggatc catggatatt
     1321 gcgtcaaagc gatacaggtc gtgatgcctc ccatcgaaag gcccgtgacc gaaacagttt
     1381 ctctttttgc caatcccgct tcaacatagg cttccacaat gtcaggaagc tcttttatat
     1441 ttttcaggac taccgaccag aattctgctg ccggttcgcc agcataggct tcctctttgc
     1501 gttcaccatg acgataggca tcggggatga tcacccgcat gccgttgctg gctaatgcat
     1561 atccgggctc caaacctctc tccttttggt ttgtgatgcc atgataaaag aacacgagcg
     1621 gagtcgtttc ttgtttcttg tcttcttcaa cgatttccag aatcggtata tcatttatga
     1681 tttttctttc gatcaaaatc acatttatcc ctctttttca gctctgatcg gctgttacag
     1741 gcaaaaatat ttttccaggt agattgccaa gccgtcctca tgattcgtat gcgtggttac
     1801 atcatctgca atgcgcttta atgccccaat accattttgc ataacaacgc catgtcccgc
     1861 aaagcgaatc atctccatat cgttgtcttc gtcaccgaag gcaagaatat cttcccgctt
     1921 gataccgtaa ttattcgcga ccacttccac gccaagcgct ttttggacac cagcggatac
     1981 gatttcgagg caaggcatcg taccgcccca tgtacggatt tcaatggaat tcccataacg
     2041 cgccaaaatc cggtcacgga tagcatgcaa attccgttcc tcggaaaata cattcatgga
     2101 agtcggattt tccgacaact tgtccttaga agtgatctgc gtcgccagca aatttttcgg
     2161 gaaatattcg ttcgatggca acgtggcggt ggatgcgaaa
//
LOCUS       DAOMUJ010000311         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14122_length_2199_cov_2.137593, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000311 DAOMUJ010000000
VERSION     DAOMUJ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14122_length_2199_cov_2.137593"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLT73_07485"
     CDS             <1..240
                     /locus_tag="PLT73_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRM20256.1"
                     /translation="QGFSADFAGALSLLTASLFGLPVSTTHTKTTAIMGVGAGRHLSS
                     VNWLTVKEMVYTWVLTFPGCGLVGYLMAKLFIQIF"
     gene            253..885
                     /locus_tag="PLT73_07490"
     CDS             253..885
                     /locus_tag="PLT73_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRM20257.1"
                     /translation="MFKKKTEKLDYFDEFIKLAEWGVQSAEFLKQVLAEYPVADLSQK
                     LDEMHEIEHNADLAKYRLMRYMYNDLLPPFDRQDVIALVTSLDHAIDMIEDVMIHIEI
                     YQPQHITPEINQFLDLVEKSAEELLTAVRLLKTYKKSQKEIHVVIDRINACEKEGDLL
                     YIHSMRDLYQSNADAVSIVILGKIYKSLEDCCDAFKDVTKFVEEIALKYA"
     gene            complement(1278..1538)
                     /locus_tag="PLT73_07495"
     CDS             complement(1278..1538)
                     /locus_tag="PLT73_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20258.1"
                     /translation="MKKKYLMIGLISFTALTTFAVAPPVDAAMMDTVTYENHGQYVRS
                     HANHLRHHTEDDHHMNGISMHARNWDGHIRYSTGELDPSLDH"
     regulatory      1765..1922
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     assembly_gap    1870..1879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2002..>2199
                     /locus_tag="PLT73_07500"
     CDS             2002..>2199
                     /locus_tag="PLT73_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20259.1"
                     /translation="MTMTKEQFERCERMEAAGGPKSQSEAMLYHQYKKQKAAVAEALK
                     MGKENYQAKLLARVAEVHRLEE"
BASE COUNT          629 a          452 c          564 g          544 t
ORIGIN      
        1 caaggattca gcgctgattt tgccggggcc ttgagcctct tgacggcttc cctgtttggg
       61 ctgccggtga gcacgaccca cacgaagacg actgccatca tgggagtagg cgcaggcagg
      121 catctgtcga gcgtaaactg gctgacggtc aaggaaatgg tttatacgtg ggtgttgacc
      181 tttccaggtt gcggccttgt cggttatctg atggcaaagc tgttcattca gatattctag
      241 ggggagccga ttatgttcaa aaagaagacg gaaaagttgg attatttcga tgagttcatc
      301 aagcttgctg aatggggcgt ccaatcggcg gaattcctga aacaggtgct tgcggaatat
      361 ccggtcgctg accttagtca aaagttggac gagatgcatg agatcgaaca caatgccgac
      421 ctcgcgaaat acagactgat gcgctatatg tacaacgatc tattgccgcc tttcgacagg
      481 caggatgtga ttgcgctggt caccagtctg gaccatgcca ttgacatgat tgaggatgtc
      541 atgatccata ttgagattta tcaaccgcag cacatcaccc cggaaatcaa tcaattcctg
      601 gatctggtcg aaaaatccgc agaagaattg ctaacggcgg tgcggctact gaaaacctac
      661 aaaaagtcac aaaaagagat ccacgtagtc atcgaccgga tcaacgcctg tgagaaggaa
      721 ggcgatctgc tctacatcca ctccatgcgc gacctctatc agagcaatgc ggatgccgtc
      781 agcatcgtta ttctgggcaa aatctataaa tcgttggaag attgctgtga cgccttcaag
      841 gacgtgacca aattcgtgga agagattgcg ttgaagtacg cttgaccagt ggtggttgtc
      901 cgataaggcg gaagaatcgg acaagcggag taagtttgat ttgctggttg tccgataaag
      961 cggagaaatc ggacaagcgg agtgagattg tcttgctggt tgtccgataa agtgggagaa
     1021 tcggacaagt agagtgagtt agtcttgctg gttgtccgat aatgtagaag aatcggacaa
     1081 tcaaagcgag tcagtagcag gaatgtccgc cataactgtt ttaaagggtt atttctgagc
     1141 gtgaaatcaa gtcccataca aaaattaata gggcggcatc aaaaccagaa tagtcctttc
     1201 tggttttgat gccgccttat ttagctatat ggtcaaaaag atgccatcaa ctttaatctg
     1261 ttgtccatat accgaaatta atggtctaag gatggatcca gttctccagt ggaataacgg
     1321 atatgtccgt cccagtttct cgcatgcata ctgatgccgt tcatgtgatg gtcgtcttct
     1381 gtgtggtgac ggagatgatt ggcgtggctg cgtacatact gaccgtgatt ttcatatgtc
     1441 accgtatcca tcatcgctgc atctacggga ggtgcgacag caaaggttgt caaggcagta
     1501 aaagaaatca ggccaatcat caaatatttt ttcttcatga ataagttctt ccttcctatg
     1561 tatactgact atagtttatg cggttgtttc agtcttgttg atcggacaaa agaaatgatt
     1621 agcaacgcgg gatcacttga tccttcttca gtatactaag gaagtgtgta gaacagatgt
     1681 agttaaaaaa tcaaatatat ggattcgcga ttagttgaat tttgcgcaag ggtatgacat
     1741 aatatatttg aaaattaaat aacttcttta tcaagagaga tcgagggact ggcccgatga
     1801 catctcagca accgaccaat gcgcgagcgc aagggcacgg tgcccgccac aatccacagc
     1861 cccaattccn nnnnnnnnng ggcacggtgc taaatcctgc agagcaatct gagagataag
     1921 aagagcaaag cgacattccc gactcttctt gtttaaagaa gggttttttg ttttcaaaaa
     1981 aacaatttgg agggaaacac gatgacaatg acaaaggaac aattcgagcg ctgcgaaaga
     2041 atggaagcgg caggcggccc aaaaagccaa tccgaggcca tgctctatca tcagtacaag
     2101 aagcaaaagg ctgctgttgc agaagcgctt aaaatgggta aggaaaacta tcaagcgaaa
     2161 ctgctggcca gagtagcgga agtgcatcgg cttgaggaa
//
LOCUS       DAOMUJ010000312         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14130_length_2198_cov_1.674288, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000312 DAOMUJ010000000
VERSION     DAOMUJ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14130_length_2198_cov_1.674288"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1141
                     /gene="deoB"
                     /locus_tag="PLT73_07505"
     CDS             2..1141
                     /gene="deoB"
                     /locus_tag="PLT73_07505"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRM20260.1"
                     /translation="MDSVGIGEAPDADKFGDVGSDTLGHIAAVAGLEIPNLETLGLGN
                     IRELSGVKSDSASLGYYTKLEEESVGKDTMTGHWEIMGLNIQSPFRVFPEGFPQELIQ
                     KIESHTGRRIIGNKPASGTEILDEFGEHQMKTGDLIVYTSADPVLQIAAHEDIIPLEE
                     LYAICEYVREITLEDPYMIGRIIARPYIGTPGHFTRTSNRHDYALDPFRKTTLDYLKE
                     SGYDVIAIGKINDIYNGQGITEYVRTKSNMDGVDQLLKVMEKDFRGLSFLNLVDFDAL
                     FGHRRDVNGYAKAIEDFDGRIPEIVTALADDDLLLITADHGNDPTFPGTDHTREYVPL
                     LAYSKKMAGRGKMNQGKFADIGATVSDNFQVAATQNGRSFLDELN"
     gene            1156..1974
                     /locus_tag="PLT73_07510"
     CDS             1156..1974
                     /locus_tag="PLT73_07510"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM20261.1"
                     /translation="MTNNQNNIRIAAKFLEEKGLLEPEFGLILGSGLGELAEEITDAI
                     AIPYEEIPYFPISTVEGHKGQLVYGNLSGKKVIAMQGRFHFYEGYGLEAVTFPIRVMK
                     QLGVHSLALTNAAGGISYAFTPGDLMLITDHINLTGQNPLIGPNDPEQGPRFTDMSQA
                     YDAEYQAKIKQVASQMGLDLKEGVYLGLTGPTYETPAEIRMCRVLGADAVGMSTVPEA
                     IVARHAGLRIFGISCITNLASGMQATLNHEEVVETTTRVKETFKTLLKNTLTAL"
     gene            2010..>2198
                     /locus_tag="PLT73_07515"
     CDS             2010..>2198
                     /locus_tag="PLT73_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRM20262.1"
                     /translation="MFISYNQSYEKIAMGLLSYVTDLKNVARLKPEMESYIENVDRKL
                     FLWKDEVTDNIVALIGVEI"
BASE COUNT          647 a          451 c          561 g          539 t
ORIGIN      
        1 tttggattca gtcggcatag gggaagcgcc ggatgccgat aagttcggcg atgttggatc
       61 ggatacgctt ggacatatcg cagcagtcgc tgggctggaa attcctaatc tggaaacatt
      121 gggattggga aacatacgcg aactcagcgg cgtaaagtct gacagcgcgt cacttggcta
      181 ctatacaaaa ctcgaggagg agtccgtcgg taaagacacc atgacaggcc actgggaaat
      241 catgggactg aacatccagt ctccattccg cgtttttcct gaaggttttc cgcaagaatt
      301 aatccagaaa atcgaatctc acacaggccg gagaattatc ggaaacaaac cggccagcgg
      361 gaccgaaatt ctggatgagt ttggtgagca ccaaatgaag accggcgatc tgatcgtcta
      421 cacatcagcg gatccggtgt tgcagattgc cgcccacgaa gacatcattc cgcttgagga
      481 attgtatgca atctgcgaat atgtcagaga gattacatta gaggatccct acatgatcgg
      541 aaggatcatc gccagaccgt atatcggaac tcccggacat tttacccgca caagcaatcg
      601 acacgattac gccttggatc cgttccggaa aacaacgctg gattacttga aggaatcggg
      661 ttatgatgtc attgccatcg gtaaaatcaa tgatatctac aacggccaag gcataacgga
      721 atatgtccgt acgaaaagta atatggacgg cgtggaccag ttgctgaaag tcatggaaaa
      781 agattttcgg ggtttgagtt tcctgaatct tgtcgatttt gatgcgctct tcggacaccg
      841 cagagacgtg aatggttacg ctaaggcaat tgaagacttt gatgggcgta tccctgaaat
      901 agtgactgct ttagcggatg atgatttgct gttgatcacc gcagatcatg gtaacgatcc
      961 aacattccca gggacagacc atacgcgcga atatgttcca ttgttggctt attcgaaaaa
     1021 aatggcgggt cgcggcaaga tgaatcaagg caaatttgcg gatatcggag cgaccgtatc
     1081 cgataatttt caggtggccg ctactcaaaa tggccgtagt tttttggatg aattgaacta
     1141 gtgaggtgtg tggatatgac aaataatcaa aataatatac gaatagccgc caaattttta
     1201 gaagaaaaag gtttattgga acctgaattc ggtttaattt tgggttcagg cttaggcgag
     1261 ttggccgaag aaatcacaga tgcaattgcg attccttatg aagaaattcc ttattttccg
     1321 atttcgacag ttgaagggca caaaggccaa cttgtttatg gaaatctgag cgggaaaaaa
     1381 gtcatcgcga tgcaggggcg cttccatttc tatgaggggt acgggctcga agcagttact
     1441 tttccgataa gggtaatgaa gcaactgggc gttcactcgt tggcgctgac aaatgccgca
     1501 ggtggcatca gttatgcctt tacaccagga gatctgatgc tgatcacgga tcacatcaat
     1561 ctgacgggcc aaaacccctt aatcggaccg aatgaccccg agcaaggacc gcgtttcaca
     1621 gacatgagcc aagcctatga tgccgaatat caagcgaaaa taaaacaagt cgcttcgcag
     1681 atgggattgg atttgaagga aggtgtttac ctcggactga caggtccgac ttatgaaaca
     1741 ccagcagaaa tccggatgtg ccgtgtactc ggggcagacg ctgtcggtat gtccaccgtg
     1801 ccggaagcca ttgtggctcg tcatgcaggt ttgcgcattt tcggtatttc ctgtataact
     1861 aatttagctt caggcatgca agcaaccctg aatcatgaag aagtcgtcga aacgaccacc
     1921 cgagtcaaag aaacattcaa aacattattg aagaatacgt tgactgctct gtaacaaacg
     1981 ggtaaataca aagtgagaag gagatcgaag tgttcatttc ttataatcaa tcatatgaaa
     2041 aaatagctat gggactgctt tcttatgtaa cagatctaaa aaatgtggcg aggctgaagc
     2101 cagaaatgga aagctacatc gaaaatgttg ataggaagct cttcttgtgg aaagatgaag
     2161 tgactgataa tattgttgct ctgataggtg tggaaata
//
LOCUS       DAOMUJ010000313         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14358_length_2171_cov_1.986767, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000313 DAOMUJ010000000
VERSION     DAOMUJ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14358_length_2171_cov_1.986767"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1964)
                     /locus_tag="PLT73_07520"
     CDS             complement(69..1964)
                     /locus_tag="PLT73_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM20263.1"
                     /translation="MHSHGHSHGHSHAHGEGDKAEIRKAVIRLIVGFGILLAAIFAPV
                     SGTVSLALFVTAYLVAGYDIVWSALLNIKHGQLFDENFLMTIATLSAFYIQEYPEAVA
                     VMLFYQLGELFQDIAVDKSRRSIAELMDIRPDYANVKTESGIEKVAPETVKIGDIILI
                     KPGEKVPLDGKIVSGTSAVDTSALTGESVPRAVKAGDSILSGFINKNGVIEVAVEKPF
                     AESTVVKILDLVQNASGRKAPTENFITKFARYYTPVVVIAAVLLAVLPPLLLPGESFQ
                     EWLYRASIFLVISCPCALVVSIPVGFFGGIGSASRKGILVKGSNFLEGLNAVKTVVMD
                     KTGTLTEGKFAVTRIESAGDLTENRLLELTAYAEMHSSHPIADSIKEAYGKTIAEEKI
                     TSTNDIPGHGLQVVVDGQEILVGNVKMMEKFGIAHAATHETGTVVHIAVDKQYAGYIL
                     IADAIKADAKAAISGLKARGIRTIMLTGDSRSVGEAVAEELGIDEVHAELLPQDKVTK
                     LEEVLARKRKGEKVIFVGDGINDTPVLARSDIGMAMGGLGSDAAIEAADIVIMDDKPS
                     KILTAIAVAEETRKIVWQNIIFAMAVKGLFLILGAFGVATMWEAVFADVGVTVLAVLN
                     SIRILKK"
BASE COUNT          499 a          620 c          510 g          542 t
ORIGIN      
        1 cgcggtgctc cggtgtccac cggagcagac aaaaataggg gccgctggcg gcccccaaat
       61 acgaacgatt atttcttcag gatccggatg ctgttcaaca cggccaaaac ggtcacgccg
      121 acatccgcga agacggcttc ccacatcgtg gcgacgccga aagcacccag aatcagaaac
      181 aaacctttca ctgccattgc aaaaataatg ttctgccaaa cgattttgcg tgtttcctca
      241 gccacggcta ttgccgtcaa aatcttggat ggcttgtcat ccatgatgac gatatccgcc
      301 gcctcgatgg ctgcatcgga tcccaatcca cccatcgcca tcccgatgtc ggaacgcgcc
      361 agtaccggcg tatcgttgat accgtcgccc acgaagatga ctttttcgcc ttttcgtttg
      421 cgtgccaaca cttcctccag cttcgtcact ttatcctgcg gaagcaattc cgcatggact
      481 tcatcaattc cgagttcttc ggcgaccgct tcgcctaccg aacgggaatc tccagtcagc
      541 atgatcgtgc ggattcctct ggctttcaat ccggagattg cggccttcgc atcagctttg
      601 atggcatcag cgatcaggat gtatcccgca tattgtttat ccacagcgat atgcacaacc
      661 gtaccggttt catgagtcgc cgcatgtgca ataccgaatt tctccatcat tttaacattg
      721 ccgacaagga tttcttgccc atcaacgacg acctgcagac cgtgaccagg tatatcattt
      781 gtggaagtaa tcttctcttc agctatcgtt ttcccgtaag cttccttgat cgaatcagca
      841 atcgggtggc tagaatgcat ttccgcatag gctgtcaatt ccagtaaacg attctccgtc
      901 agatcaccgg cgctttcgat ccgggttacg gcgaacttgc cttccgttaa cgttccggtc
      961 ttgtccatca cgaccgtttt gaccgcattc aggccttcca gaaagttgct ccctttgacg
     1021 aggatgcctt tgcgcgaggc tgaaccgatg ccgccgaaga agccaaccgg aatcgaaacg
     1081 accaaagcac acggacagga gatgaccaga aagatgctgg cccggtaaag ccattcctga
     1141 aagctttcac ccggtaacaa aagcggcggc agcaccgcca gcaaaacggc tgcgataaca
     1201 acaaccggcg tgtagtagcg ggcgaatttg gtgatgaagt tttccgtcgg cgcttttcgg
     1261 ccgctggcgt tctgcaccaa atccaaaatc ttgacgactg tggactcagc aaacggcttt
     1321 tcgactgcga cttcgatgac gccgtttttg ttgataaaac cgctcaaaat gctatcacca
     1381 gctttcacag cccgtggaac agactctccg gtcaatgccg aggtgtccac agccgaggtt
     1441 ccgctcacaa tttttccgtc caagggtact ttttctccag gcttgatcag gatgatgtcg
     1501 ccgattttaa cggtttccgg tgcgactttt tcgataccgg attctgtttt cacatttgcg
     1561 taatcaggcc ggatgtccat cagttcagcg atggaacggc gggatttgtc gacggcgata
     1621 tcctggaaca gttcgcccag ttgatagaaa agcatgacag ccaccgcttc cggatactcc
     1681 tggatataga aagcgctcag cgtggcgatc gtcatcagga agttctcatc gaataattgg
     1741 ccgtgcttga tgttcaggag cgcgctccaa acgatgtcgt agcctgcaac caagtacgcg
     1801 gtcacaaaca atgccaacga aaccgttccg ctgacgggtg caaaaattgc cgccagcagg
     1861 atcccaaatc cgacgatcaa tcggataacg gctttccgga tttcagcctt atccccttca
     1921 ccatgcgcat gactatgtcc atggctatgt ccatggctat gcacttcagc atcatcggaa
     1981 gtcgggccgt gatcgtcgcg aacattttct gcatattttg catcagccgt ttgggccgca
     2041 actagcggca gaccatttaa gcctatcggt tcaccgcttt ttttcagagt cggatggatg
     2101 tccggctcca gtatactgag cttttgtttc actttcggca aaacttcagc ctcttgatcc
     2161 tcttctattt c
//
LOCUS       DAOMUJ010000314         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14367_length_2170_cov_2.459574, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000314 DAOMUJ010000000
VERSION     DAOMUJ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_2170_cov_2.459574"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(9..276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            540..1697
                     /locus_tag="PLT73_07525"
     CDS             540..1697
                     /locus_tag="PLT73_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM20264.1"
                     /translation="MALTTNELFSRIKPSQIREFDELFRSVEGCIPMTIGEPDFDMPE
                     NVKRAAIKAIEDNASHYAHTSGEIGVRKAVSEFLEKQYDLHYDPETEIVMTVGATEGL
                     FAAAFGLLNPGDQVIIPSPYFPLYSYAVELNRGECILVDTSDTGFLMTPELLHKTMAE
                     NKNVKALFLNYPSNPTGATYTKDELIALADAIKQYDIFVLSDEIYSEITYGEKHTSIA
                     TLLRDRTILFQGASKAFAMTGWRVGVLAADAKWMKTLFLAHQQLVTTGVTVSNRAAEE
                     AFRNSAPDVEKMRSEYEKRRNILVPALQEAGFEVPALKGAFYAFAKIPAKFGTDDKAF
                     CREIGMNAKVGMLPGSIFGPGGEGYVRMSYALSTEMVAEAAERLKTYIASK"
     gene            1765..1959
                     /locus_tag="PLT73_07530"
     CDS             1765..1959
                     /locus_tag="PLT73_07530"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HRM20265.1"
                     /translation="MQMPYVHIELLEGRTDEQKQGLMRDVVDAVVKNANVSKESVHVI
                     LNEISKQHLTKNGEFIGEKA"
BASE COUNT          614 a          514 c          540 g          502 t
ORIGIN      
        1 atgcgcaaga taaggacgag aggtcaaagt tcaatctcgt gttaccacct tagttcacga
       61 aagcatcgct gcatccgcct tagccagtgc gcctgcaaat tgctacaagt cagactgtga
      121 cactgtaacg ggcgttcccg tcctgtctta agaaattact cttcggacaa gccactctga
      181 gaccatcttc aaccaacact tccttgcttg ttttcaccta accaagctct ctaatgaagg
      241 agtggaatga tttactcttc tcttcgctgt gtttttgatg tgattaatat acccaatccc
      301 gctttctttg tcaacagata agattagaaa aaaacagaaa attttcagag attctcattt
      361 tgctagatag aaaacgctct ctttaaatgg gtgatgccat ggtgcgcttg acttatgggc
      421 agaatagtcg tattctgatt aatattaaaa ttatgtgaga aaaaggatat gaaccaataa
      481 aaaagcatga tccgagggga ttccggcggt gatgcacact tggatggagg atgtactaca
      541 tggctttgac aacgaatgaa ctattttccc gcatcaaacc ttcgcaaatc cgcgaatttg
      601 atgaattatt ccgcagcgtg gaaggctgca ttccgatgac gatcggcgaa ccggattttg
      661 atatgcccga aaacgtcaag cgagccgcca tcaaggcgat cgaggacaac gcctcccatt
      721 atgcgcacac ttccggcgaa atcggcgtcc ggaaagcagt cagcgaattc ctggaaaagc
      781 aatatgatct gcactacgat cctgaaacgg agatcgtgat gacggtgggt gccactgaag
      841 ggctcttcgc cgcggctttc ggcttgttga acccgggcga tcaggtcatc atcccgtcgc
      901 cgtactttcc cttatacagc tatgcggtgg aactgaaccg aggcgaatgc atcctcgtcg
      961 atacttccga caccggcttt ttgatgacac cggagctgct gcacaaaacg atggccgaaa
     1021 acaaaaatgt caaagcgctc tttttgaact acccgagcaa tccgacaggc gccacctata
     1081 caaaagatga actgatcgct ttggccgatg cgatcaagca gtacgacatc tttgtgctga
     1141 gcgatgaaat ctacagcgag atcacttacg gggaaaagca tacgtcgatc gcaaccctgc
     1201 tgcgcgaccg cacgattttg ttccaagggg cttcgaaagc ttttgcgatg acaggctggc
     1261 gtgtcggtgt gctcgcagcg gatgcgaaat ggatgaagac cctgttcttg gcccatcagc
     1321 aattggtgac gaccggcgta acggtttcca accgggctgc cgaggaggca ttccgcaaca
     1381 gcgcaccgga tgtcgaaaag atgcgttccg aatatgaaaa gagaagaaac atcctcgttc
     1441 ccgctttaca ggaggcaggc tttgaggttc ccgcactgaa aggggccttc tatgcttttg
     1501 cgaagattcc ggcgaagttc ggtacggatg ataaggcctt ctgccgcgaa atcggcatga
     1561 acgccaaagt gggcatgctg ccgggatcga tcttcggacc cggcggagag ggctatgtcc
     1621 gcatgagcta cgcgctgagc acggagatgg tcgctgaggc ggctgagcgc ctgaagacct
     1681 acatcgcctc caaatagagg tgcaatcgat aaaaataatt gctaaaatat aactgagttg
     1741 aaaccataaa gcgaaaagag ggaaatgcag atgccatatg ttcacatcga attgctggaa
     1801 ggcagaaccg acgagcagaa acaaggcttg atgcgcgatg tcgtggatgc cgtcgtaaaa
     1861 aacgccaatg tatcgaaaga aagcgtacac gtgatcctga atgagatctc aaaacaacat
     1921 ttgaccaaaa acggcgaatt cataggcgaa aaagcttaag gataccgtaa aataagccaa
     1981 ggccgggata aaatatccgg ccttggctta ttttttgatc gtaattgatt tgctatagaa
     2041 accatatatc gccgatgatc agacggatcc aattcatggg tgatgccccc tttcacagaa
     2101 caggcaagct tgcgaccgga aacgctgcat acagaagcag ctatttttca tgaaaagtgc
     2161 ttttgacata
//
LOCUS       DAOMUJ010000315         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14372_length_2169_cov_2.288079, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000315 DAOMUJ010000000
VERSION     DAOMUJ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14372_length_2169_cov_2.288079"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..456
                     /locus_tag="PLT73_07535"
     CDS             82..456
                     /locus_tag="PLT73_07535"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM20266.1"
                     /translation="MFLFFDVLPFRKQLEYNQTITCSRCGHFGRYEVYLVGNRFRLFF
                     IPVITFGKKYLVRTTCCDTWYLLDPLVGKAIEWKETVSIRDSDLQMYQTGEPLESRCP
                     NCGASYPHGSNYCPNCGVKVEG"
     gene            573..1400
                     /locus_tag="PLT73_07540"
     CDS             573..1400
                     /locus_tag="PLT73_07540"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM20267.1"
                     /translation="MLTNKEIRSTAREYLRGNYKMAVVNLILITIANSVMQSVVRTLT
                     GESALNMQMTGETLPNWDSVFSMQSSPFQTTLNVVLSIIVALIIGSMQMGNEWGYLDM
                     LDGTPLSSGHLLKPFENQLFKTLGVLVLQAVYVILWSVLLIIPGIMKLYALALSNFIY
                     FDNPDMKTTDILRQSEAFMKGKKMRLFQLDLTYFVVYLIPVVLFFGVGIAAFNIYAGA
                     TTADSDALLYQALLLVGLMLAAFAIFGILTFIVEPRRKAAHAVFYSEVLKEENLIIG"
     gene            1447..>2169
                     /locus_tag="PLT73_07545"
     CDS             1447..>2169
                     /locus_tag="PLT73_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM20268.1"
                     /translation="MEPRIKREAPSITTDLRSDTVQVPSVIRNCSGIRIFGKRIKSLI
                     FTTDIAIILNNDADAVIAVYPFTPHPAVIQSIANVSTIPILSGVGGGTTQGKRSANIS
                     LFSEAQGSLAVVVNAPTPIDTIKQIEETVDIPIVCTIVTEYMDIQERLDAGVDILNVS
                     GGKDTAYIVSRIREKYPDVPIIATGGPTDETILAAINAGANAISWTPPSNGELFRRKM
                     DKYRDKEREKFMQEHQGMTIEEV"
BASE COUNT          592 a          487 c          526 g          564 t
ORIGIN      
        1 aatcattttt gtctgctttg acgatgcgac agtgcgcgtc tatgagcagg catttgaaga
       61 tttggaaagg agaactgact gatgttttta ttttttgacg tattgccgtt ccgcaagcag
      121 cttgaataca accagacgat cacgtgcagc cgttgcggtc attttggccg ctatgaggtc
      181 tatctcgtgg gcaatcgttt ccgcctgttt ttcatcccgg tcatcacctt cgggaaaaaa
      241 taccttgtcc gcacaacctg ttgcgacaca tggtacctgc tggatccgct tgtaggaaaa
      301 gcgattgaat ggaaagaaac ggtttcgatc cgcgacagcg atctgcagat gtatcaaaca
      361 ggtgaaccgc ttgaaagcag atgccccaac tgcggtgcct cttatcctca tggctccaat
      421 tattgtccaa attgcggagt gaaagtggag ggataggaaa gcggacacat catcaagtga
      481 ataaatgaaa gacaattaac tgaaagcgtt gcaaattgca caattatggt atactacaat
      541 tatcgaaatt tgtaaggggt ggtcataatc atatgttaac gaataaagaa atcagatcaa
      601 ctgctagaga atatttacgc ggcaattata aaatggcggt cgtcaacttg atccttatca
      661 caatcgcgaa cagcgtcatg caatcggtag tccggacgtt gacaggcgaa agtgctctga
      721 atatgcagat gacgggggaa accttgccga attgggattc cgtcttttca atgcaatcct
      781 cacctttcca aacgactttg aatgtcgtcc tttcgatcat cgtggcgttg atcatcggct
      841 ccatgcagat ggggaacgaa tggggttatc tggatatgct ggacggtaca ccgctttcgt
      901 cgggccatct gctcaaaccg tttgaaaacc agttgttcaa aaccctcggc gttctggtgc
      961 tgcaggcggt ttatgtcatt ctctggtccg tattgctgat cataccgggt atcatgaagc
     1021 tttacgcttt ggcattatcg aacttcattt atttcgataa tccggatatg aaaacgacag
     1081 acatcctgcg acaaagcgaa gctttcatga aaggcaaaaa gatgagactt ttccaattgg
     1141 atctgactta ttttgttgtc tacctgattc ctgtggtatt gttctttggt gtcggaatag
     1201 ctgcattcaa catatatgcc ggagcgacaa cggctgattc cgatgcatta ctgtaccaag
     1261 cgttgttgct ggtaggcttg atgcttgctg cattcgccat tttcggcatc cttaccttca
     1321 tcgttgagcc gagaagaaaa gcagcgcatg cggtattcta ttcggaagtc ctgaaggaag
     1381 aaaacctaat cattggctga tcagccaaga tcatttcata aaaatcaaca gtaaaggagc
     1441 gaaagaatgg aaccaagaat caaacgagaa gcgccaagca tcacaaccga tttgcgcagt
     1501 gatacagtcc aagtcccaag tgtgatccgg aattgttccg gaatccggat ttttggaaaa
     1561 cgtatcaaat cgttgatctt tactacggat attgcgatta tcctgaataa cgatgcggat
     1621 gccgtcatcg cggtttatcc atttaccccg catccggcgg tcatccaaag tatagccaat
     1681 gtctcgacca ttccgatcct ttcgggcgtc ggaggtggga cgacgcaagg taaacgtagt
     1741 gccaatatct ctttgttttc tgaagcgcaa ggatccttgg ctgtagtcgt gaacgcgccg
     1801 acaccgatcg atacgatcaa acagattgaa gaaactgtgg atattccgat cgtctgcacc
     1861 atcgtgacgg aatacatgga tatccaggag cgtttggatg ctggtgttga catcctgaat
     1921 gtcagcggcg gaaaagacac cgcttatatc gtaagccgca tccgcgaaaa gtatccggat
     1981 gttccgatta tcgcaaccgg aggacctaca gatgagacga tattggctgc aatcaatgcc
     2041 ggcgccaatg ccatcagctg gacgcctccc tccaacggtg aattgttccg ccgcaagatg
     2101 gacaagtacc gcgacaaaga gcgcgaaaaa tttatgcagg aacatcaagg tatgacgatc
     2161 gaagaagtc
//
LOCUS       DAOMUJ010000316         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14444_length_2161_cov_3.533238, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000316 DAOMUJ010000000
VERSION     DAOMUJ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14444_length_2161_cov_3.533238"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..610
                     /locus_tag="PLT73_07550"
     CDS             269..610
                     /locus_tag="PLT73_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRM20269.1"
                     /translation="MIITTTAHIEGKKITAYKGIVFGEVITGINVFKDFGAGIRNIVG
                     GRSKSYEDELTVARENALAEMEDRAVAKGANAIVGMKMDYEVLGADNGMLMVTCSGTA
                     VKLMDESEYLG"
     gene            complement(662..>2161)
                     /locus_tag="PLT73_07555"
     CDS             complement(662..>2161)
                     /locus_tag="PLT73_07555"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM20270.1"
                     /translation="ECLARITVTNGTFEAKYQHEFESKQEMKTFIATHGLAAEMPAFI
                     KRYEANENKRELAPRLYSLSDLQRAANAKFKMGAKDTLATVQSLYEAKLLSYPRTDAT
                     VITKNEFAYLKDNLSGYLGILGHTIDKPNLTPRKKYVDDAKVQEHYAIIPTKKVPDQA
                     RIAKLNVNQQKIYDLVLRRTLAMFEEDFIYDEPTIITDIGGLDFVASGKIIKNLGWKK
                     LEGNTRKKGEPEDKILPMVTVGETGTAILATKEKKTTPPKPYTEGTLIAAMKNAGKEV
                     DDAEDKAILKETHGIGTEATRAGVIENLKDRGYITASKNQLAITEKGTLLCEAVAGNK
                     MSDVAFTAEWEKYLAKIHKGEGDQAYFLENIKHFVLEILGTKNEMLQSEGIAERIDAA
                     GDGAVVGVCPVCGKEAIIKGSYLQCQDYGEACSFRISRYIARRYLSPEEAKELLARKE
                     TKRLSGFKKKDGKSFSTALLLGKDEAGNYAVSFKPFAPSKKKPVRKKTQ"
BASE COUNT          478 a          543 c          525 g          615 t
ORIGIN      
        1 cacgaaatgg cgagaggcaa gccgtgactt cagtcattgg tagttgacag taaccgtccc
       61 ttctgtttcg agtcttctct gattttacca ttaagcaaca atctgtacaa ttttacccac
      121 aacatttttc tgcccagcgg ccaccaagat ggcaggtttc tgtatctttt caagccgaac
      181 ttttgatata atgaaggcat caccagaaag ttataatcga tttgttgaac tgattgtttc
      241 accgtcagga gaaaggatgg taagtcacat gattattaca acaaccgctc atatcgaggg
      301 caaaaaaatt actgcctata aaggcatcgt tttcggagaa gtcatcacag gcatcaacgt
      361 gttcaaagat ttcggagccg gcatccgcaa catcgttggc ggacgttcaa agagttacga
      421 ggatgaattg actgttgcac gcgaaaatgc cttggcagaa atggaagatc gtgcagtcgc
      481 aaaaggcgcg aacgccatcg tcggcatgaa aatggattac gaagtactgg gagccgataa
      541 tggcatgctg atggtcacct gcagtggtac agctgtgaaa ttgatggatg aatcagaata
      601 tttaggctaa aaaaatcaga aaaggtatcc gggaagcaat tttccggata cctttttctg
      661 ttcattgtgt ttttttgcgt accggttttt tcttactcgg agcgaacggt ttgaaggaaa
      721 cggcgtaatt gccggcttcg tctttcccca agagcaatgc cgtcgaaaaa ctcttgccgt
      781 cttttttctt gaaaccggaa aggcgcttcg tttcttttcg ggccaaaagc tctttggctt
      841 cctccgggga cagatagcgg cgggcgatat agcgggaaat ccggaatgaa cacgcctcgc
      901 cgtagtcctg gcattgtagg taactgccct tgatgatcgc ttctttcccg cagaccggac
      961 aaacgcctac gaccgcaccg tcgcctgcag catcgatgcg ctccgcgatc ccttcggact
     1021 gcagcatctc gttcttcgta ccgaggattt ccaggacgaa gtgtttgatg ttctccagga
     1081 aataagcctg gtcgccctcg cctttatgga tttttgccaa atacttttcc cattctgccg
     1141 tgaaggcgac atcgctcatc ttgttgccgg ccacggcctc acaaagcaga gtcccctttt
     1201 cggtgattgc cagctgattc ttcgaagccg ttatgtagcc cctgtccttc aaattttcga
     1261 tgaccccagc gcgggtagct tcggttccga taccgtgcgt ttccttcagg atcgccttgt
     1321 cttcggcatc atccacttct ttgccggcgt tcttcatggc tgcgatcagc gtgccttctg
     1381 tgtaaggctt aggaggggta gtcttcttct ccttcgtggc caaaatggct gttccggttt
     1441 cccctaccgt aaccatcggc aggattttgt cctccggttc gccttttttc cttgtgttgc
     1501 cttccagctt cttccatccg agattcttga tgatcttacc ggatgccaca aagtccagtc
     1561 cgccgatatc cgtaatgatg gtcggttcat cgtagatgaa atcctcttcg aacatcgcca
     1621 aggtccggcg caggacgaga tcataaattt tctgctggtt cacattcaat ttcgcgatcc
     1681 gggcctgatc cggcactttt ttcgtcggga tgatggcata atgttcctgt actttggcat
     1741 catcgacgta ttttttgcgc ggagtcaagt tcggcttgtc gatggtatga cctaggatgc
     1801 ccagatagcc ggaaaggtta tccttcaaat aggcaaactc atttttggtg atgaccgtcg
     1861 cgtccgtccg cggataggaa agcagttttg cttcatacaa agactgcacg gttgccaatg
     1921 tgtctttcgc acccatcttg aatttggcat tggctgcccg ctgcagatcg ctgagcgaat
     1981 acagacgcgg tgccaactcg cgtttgttct cgttggcttc atagcgtttg atgaaggctg
     2041 gcatctcagc cgccaagccg tgcgtcgcga tgaaggtttt catttcttgt tttgattcaa
     2101 attcatgctg atacttcgct tcgaatgtcc cgttcgtgac cgtgatgcgg gccaaacact
     2161 c
//
LOCUS       DAOMUJ010000317         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14552_length_2148_cov_2.305303, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000317 DAOMUJ010000000
VERSION     DAOMUJ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14552_length_2148_cov_2.305303"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..873
                     /gene="thiM"
                     /locus_tag="PLT73_07560"
     CDS             88..873
                     /gene="thiM"
                     /locus_tag="PLT73_07560"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRM20271.1"
                     /translation="MFKMLFENVQQQTPLVHCITNYVTVNDVANVLLAAGGSPIMADA
                     PEEVADTLNSRAVESMLLAGRQANALAHPVVLDPVGAGASPFRTETTFNLLEDIAFTV
                     IKGNISEMKTIHSGSGTTKGVDADISDAVTEETLEATVAFAKRLADRNEAVIAITGAI
                     DIVADRDKAYIIRNGHPQMAKVSGTGCMLSAVIAAYCGANPEQLLDATAAAVCLMGVS
                     GELAYERMIRNAAGTSSYRSYLIDEISKMSAKKLEGGAKIENR"
     gene            860..1501
                     /gene="thiE"
                     /locus_tag="PLT73_07565"
     CDS             860..1501
                     /gene="thiE"
                     /locus_tag="PLT73_07565"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM20272.1"
                     /translation="MRIDKNDLLLYAVTDRQWLGEASLAEQLEEAIQAGVTVVQLREK
                     ELDFDAFVSLGKELQAVAKRHRIPFIINDAVDVALALDADGVHVGQSDMQAGDVRSRI
                     GEGKILGVSAHSVEEALAAERNGADYLGVGAIYGTATKPDANTLSIETLQQICQAVSI
                     PVVGIGGIAEGNILNLNGSGIDGVAVVSAIFAQPDITAAVKRLRLLAEAMVQP"
     gene            1498..2139
                     /locus_tag="PLT73_07570"
     CDS             1498..2139
                     /locus_tag="PLT73_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM20273.1"
                     /translation="MKLEAAIFDLDGTLLDSMPIWQGLGEKYLLQKKLQPAPGLHDAL
                     KTMSLPQAAQHFRAAYGLHEDEETIIGEIETLIADLYRYEVPLKAGAAEYLQTLQEQN
                     VKMCIATATERNLAESALERLGIREYFSTVLTSSEVKTGKDNPLIYQRAFEHLGAPLT
                     KTVIFEDALHAIETATAAGFRVVGVYDETAAQDRERIEALTEKYIYSFSDWKG"
BASE COUNT          516 a          545 c          644 g          443 t
ORIGIN      
        1 tgaatgattc tattcagcgg gaaatgccta tttcggacat ttcccgcttt ttttgtcgca
       61 tcaaataaat tatttggagg tactcccatg ttcaaaatgc tattcgaaaa tgtccagcaa
      121 cagacgccgc tggtccattg catcaccaat tacgtaaccg tcaatgatgt cgccaatgtc
      181 ctgctggctg ccggtgggtc gccgatcatg gcggatgccc ccgaggaagt cgcggacacc
      241 ttgaacagcc gagccgtgga gtcgatgctg ctggccgggc gccaagccaa tgcgctcgcc
      301 catcccgttg ttttggatcc ggtcggtgcg ggggcatccc ctttcaggac cgaaacgacc
      361 ttcaacttgc tggaagacat tgccttcacc gtgatcaaag ggaacatttc ggaaatgaaa
      421 acgatccatt ccggcagcgg cacgacgaaa ggcgtcgatg cagacatcag cgatgccgtg
      481 accgaggaga cgctcgaggc aacggtcgct ttcgccaaac gcttggccga ccgcaatgag
      541 gcggtcattg cgatcaccgg cgcaatcgat atcgttgcgg accgggacaa agcctacatc
      601 atccgcaacg gacatccgca gatggcgaag gtgtcgggaa ccgggtgcat gctgtcggca
      661 gtgattgcag cctattgtgg agccaacccg gagcagttgc tggatgctac cgcggcagcg
      721 gtatgcctga tgggagtcag cggcgagctg gcttatgaaa ggatgatccg caatgcggca
      781 gggacttcat cctatcgcag ctacctcatc gatgaaatca gcaagatgtc ggccaaaaaa
      841 ctggaaggag gcgctaaaat tgagaataga taagaacgat ttgctgcttt atgcggttac
      901 cgatcgccaa tggctggggg aagcatcctt ggcggaacag cttgaagaag ccatccaggc
      961 gggcgttact gtcgtgcaat tgcgcgagaa ggaactggat tttgatgcct ttgtatccct
     1021 tggcaaggaa ctgcaggctg tggccaaacg ccaccgcatt cccttcatca tcaatgatgc
     1081 ggtcgatgtg gccttggcgt tggatgcgga cggtgtacat gtggggcaga gcgatatgca
     1141 ggcaggagat gtccgcagcc ggatcggcga agggaaaatc ctgggtgtct ctgcccattc
     1201 cgtcgaagag gcgctggcgg ccgaacggaa cggggccgac tatctgggcg tcggtgccat
     1261 ctacggtaca gcaacgaagc cggatgccaa tacgctatcg atcgagacgc tgcagcagat
     1321 ctgccaggcg gtgagcatac ccgttgtggg gatcggaggc attgctgaag ggaatatcct
     1381 caatctgaac ggcagcggca tcgacggggt tgccgtcgta tccgccatct ttgcgcagcc
     1441 ggatataacc gcggcagtga agcgactgcg cctgttagcc gaagcgatgg tgcaaccatg
     1501 aaactggaag cagccatttt cgatctggac gggacactac tggattccat gccgatttgg
     1561 caaggcttgg gagaaaaata tttgcttcag aaaaagctgc agccggctcc tggattgcat
     1621 gatgcgttga agacgatgag cctcccgcag gccgcacaac attttcgagc ggcctacgga
     1681 ttgcacgaag atgaggagac aatcatcggg gaaatcgaga cgctgattgc ggatctttac
     1741 cgttatgaag tcccgcttaa agccggcgca gcggaatacc tgcagacctt gcaggagcag
     1801 aatgtgaaaa tgtgcatcgc caccgcgacc gaaaggaatt tggccgagag cgccttggag
     1861 cgtttgggca tccgggaata tttcagcact gtcctgacca gcagtgaagt taagacgggc
     1921 aaagacaatc cgctcattta tcagcgtgcg ttcgaacatc tcggggctcc tctcacgaag
     1981 accgtaatct ttgaggatgc cctgcacgcc atcgaaacgg caacggcagc cggttttcgg
     2041 gtagtcggcg tctatgatga gacggcggca caggataggg agcgcatcga agcgttgacg
     2101 gagaagtaca tctattcatt ttcggattgg aaaggatgat aaaaacat
//
LOCUS       DAOMUJ010000318         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14622_length_2140_cov_2.480576, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000318 DAOMUJ010000000
VERSION     DAOMUJ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_2140_cov_2.480576"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..2071
                     /gene="typA"
                     /locus_tag="PLT73_07575"
     CDS             233..2071
                     /gene="typA"
                     /locus_tag="PLT73_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRM20274.1"
                     /translation="MEFRKDIRNVAIIAHVDHGKTTLVDELLKQSDTLNARTELMERA
                     MDSNDIEKERGITILAKNTAVQYKGTRVNIMDTPGHADFGGEVERIMRMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQKLIPIVVVNKIDKPTARPAEVVDEVLELFIELGADDEQL
                     EFPVVYASAMNGTSSMSDDPADQEDTMDNVFNAIIEHIPAPVDNSAEPLQFQVALLDY
                     SDFVGRIGIGRVFRGTIKVGDQVSLLKLDGSFKNFRVSKLFGYFGLDRVEIEEAKAGD
                     LIAISGMEDIFVGETVTPVDHREALPVLHIDEPTLQMTFLTNNSPFAGREGKWVTSRK
                     IEERLMKQLHTDVSLRVDPTDSPDAWVVSGRGELHLSILIENMRREGYELQVSRPEVI
                     IREVDGVRCEPFERVQIDTPEEYMGSVIETISQRKAEMQDMQNSGNGQVRIIFLVPSR
                     GLIGYSTEFLSITRGYGIMAHTFEAYLPELPGKIGGRSKGALVSTETGKTTTYGIMGV
                     EDRGTIFIEPGVDIYEGMIVGENARDNDIAVNITRAKQMTNIRSANKDSTNVIKRPRT
                     LTLEESLEFMGEDEYCEVTPESVRLRKQILDKNEREKAAKRKKKAE"
BASE COUNT          666 a          429 c          492 g          553 t
ORIGIN      
        1 ttttaagaaa gccattacat tgaacttttt tctttccaaa tgattctgga ggtgatagac
       61 tggacaagtt gatagttgtc ttcatgagtt ttcagcgggt tttcacctat tccgcaaatg
      121 aaagctgatg ggaagttcaa attcagaatt gtgatcgact atataatggc agtagcagtt
      181 ggcatatcaa acagcagtga aaaacaagta aaattttgga ggaaaacaac aaatggaatt
      241 tagaaaagat attcgtaatg ttgcaatcat cgcccacgtt gaccatggta aaacaacatt
      301 agtagatgaa ttgttgaaac aatcggatac attgaacgct agaactgagc tcatggaacg
      361 cgcaatggat tccaacgata ttgagaaaga acgtggtatc acaatcttag caaaaaatac
      421 tgcggtacaa tataaaggca cacgtgtcaa catcatggac acaccaggac acgcggactt
      481 cggtggtgaa gtagaacgta tcatgcgtat ggttgatggc gtagtgcttg ttgtggatgc
      541 ttatgaagga acaatgccac aaacacgttt tgtactaaaa aaagctttag agcaaaaact
      601 tattccaatc gttgtagtaa acaaaattga caaaccgact gctagaccag ccgaagttgt
      661 ggatgaagtc ctggaattat tcatcgaatt aggcgcggat gatgaacaat tggaattccc
      721 tgtcgtttac gcttccgcaa tgaatggtac atcaagcatg tcagacgatc cagctgatca
      781 agaagataca atggacaacg tgttcaatgc gatcattgaa cacataccag ctccagttga
      841 caattcagct gaaccgttac aattccaagt agctttactt gattacagtg actttgttgg
      901 ccgtatcggt atcggacgcg tattccgtgg cacaatcaaa gttggagatc aagtatcctt
      961 acttaaattg gacggatcat tcaagaattt tcgtgtatca aaattgttcg gttacttcgg
     1021 tctggatcgc gtcgaaatcg aagaggcgaa agctggcgat ttgattgcca tttccggtat
     1081 ggaagacatc ttcgtcggtg aaacggtgac tcctgtcgac catcgcgaag ctttgccggt
     1141 tctgcatatc gacgaaccta cattgcaaat gactttcttg acaaacaact ctcctttcgc
     1201 aggccgtgaa ggtaaatggg ttacttcccg taagattgaa gagcgtttga tgaagcaatt
     1261 gcacactgat gtatccttgc gtgttgatcc aaccgactct cccgatgctt gggtcgtttc
     1321 cggacgtggc gaattgcatt tgtccatcct gatcgaaaat atgcgtcgtg aaggctacga
     1381 attacaagta tcccgtccag aagttatcat cagagaagtt gatggcgtaa gatgcgagcc
     1441 ttttgagcgt gttcaaattg acacccctga agaatatatg ggttccgtaa tcgaaacgat
     1501 cagccaacgc aaagctgaaa tgcaagatat gcaaaacagc ggcaacggcc aagtccgtat
     1561 cattttcctt gttccttcac gtggactgat cggttactct actgaattct tatccatcac
     1621 tcgcggatat ggtatcatgg cgcatacgtt cgaagcgtat ttgccagagt tgccaggaaa
     1681 aatcggcgga cgcagcaaag gcgcacttgt ttctactgaa acaggcaaaa caacaaccta
     1741 cggcatcatg ggcgttgagg atcgcggaac gatcttcatc gagcctggtg tggatattta
     1801 cgaaggtatg attgtcggcg aaaacgctcg tgataacgat atcgctgtaa atatcacaag
     1861 agcgaagcaa atgacgaata tccgttccgc aaataaagat tctactaacg taatcaaacg
     1921 tccgcgtaca ttgacgcttg aagaatcgct tgagttcatg ggagaagatg agtattgcga
     1981 agtaacacct gaaagtgtgc gactgcgtaa acaaattctg gataaaaatg agcgtgaaaa
     2041 agctgctaaa cgcaagaaaa aagcagaata aacctataac aatttctaag aaaagctgaa
     2101 cgggttcgtt cagccctgat taaggaaaca ctgtgaccta
//
LOCUS       DAOMUJ010000319         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14727_length_2129_cov_2.322083, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000319 DAOMUJ010000000
VERSION     DAOMUJ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14727_length_2129_cov_2.322083"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..954)
                     /locus_tag="PLT73_07580"
     CDS             complement(250..954)
                     /locus_tag="PLT73_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRM20275.1"
                     /translation="MYTISTLEITLRLLAAVFVGGVIGYEREENRQAAGLRTNIIVSV
                     SACLITLIQLEIGYYSLNLAIANPDIASSITVDFTRIIAQIVSGIGFLGAGTILITKR
                     DTVTGLTSAATIWTVAGLGIAVGMGYYFLSVIGCLILYIVLHIIKKIRTSIGGLHVEV
                     HYRHQDLKKVIELFLSENNIETSDMKFLIENPESEHPNYIVIYGIDLPHYYKKKDLVN
                     DLLNMSKDITNVTFDQ"
     gene            1124..>1504
                     /locus_tag="PLT73_07585"
     CDS             1124..>1504
                     /locus_tag="PLT73_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRM20276.1"
                     /translation="MSKKEWLAGMKAIYPVCFGYIPMGLACGVLLQQAGYHWLAVMLM
                     SLLIYGGASQFMIASMTVAGAGLLEMVIMIFFINLRHLLMSSSLAHRIREKSTAFSIF
                     FAQTITDESFAVNTLYFKTDGMWNP"
     assembly_gap    1505..1514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1515..1919
                     /locus_tag="PLT73_07590"
     CDS             <1515..1919
                     /locus_tag="PLT73_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20277.1"
                     /translation="LHPLSLEPQGLQNSVIDSFKTDGMWNPTKAMAANVTAYSTWIIS
                     TGIGAMAGKSIALSPVVMNYILIAMFIFLLISQIENRIVFWTAVFSGITAVVLMNLLR
                     HNIAIVLTSILASGFGLYLQNKKDDKEGILRG"
     gene            1912..>2129
                     /locus_tag="PLT73_07595"
     CDS             1912..>2129
                     /locus_tag="PLT73_07595"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRM20278.1"
                     /translation="MDDKTLWLILGGGLVTFLPRWLPIVALKNARIPIWFQKWMSFLP
                     VSIFSALIASDIFFWEGNVSLNILDNAKL"
BASE COUNT          521 a          488 c          544 g          566 t
ORIGIN      
        1 gtgtcgaact ccggtgaacg ggccatccgc cggaattagt tggtaatgtt cagcttcagc
       61 ttcgacaaag cctgccccac cggaggacac aagcagaatg tggggtgaaa tccggcgaaa
      121 ccagcgttaa ccggagcagg gcagttccat aaaaagtttc aacataaatg aaaagaagca
      181 accgaaactc gcgctccggt tgcttctttt gctgttccgg ctgagatgcc ggatgatatt
      241 tgccattcac tattggtcga aagtcacgtt tgtgatgtct ttactcatat tcaacagatc
      301 attcaccaga tcctttttct tgtagtaatg cggcaaatca atcccataga tgacgatgta
      361 gttcgggtgc tcggactccg gattctcaat cagaaacttc atgtccgaag tctcaatatt
      421 gttttcggaa aggaacaatt caatcacttt tttcaaatcc tggtgccggt aatggacctc
      481 cacatgcagc ccacctatgg aagtcctgat tttcttgatg atgtgcagga ctatatacag
      541 tatcagacag cctatgacag aaagaaaata gtagcccatt ccgacagcaa ttccgagtcc
      601 cgcgaccgtc cagatggttg ccgctgaagt aaggccggta accgtatccc gtttggtgat
      661 gaggatcgtt ccggcgccga gaaacccgat tccgctgaca atctgggcga tgatgcgggt
      721 gaagtcgacg gtgatgctgg atgcgatatc cggattggcg atagccaagt tcaggctgta
      781 ataaccaatt tcgagctgga tcaaagtgat gaggcaggcg gacacggaca cgatgatgtt
      841 cgtccgcaga cctgcagctt ggcggttctc ttcgcgttca tagccgatga cgccgccgac
      901 aaagaccgcc gcaagcaagc gcaatgtgat ttccaatgtg ctgattgtgt acaccgtaat
      961 tccccccttt caaaatagta tatttcctcc attttaaagg atgcggtgta ttctgtaaaa
     1021 ggaaatgacg attttctgaa aatgtgcaga atcacgccga atattgcttc atcatgtgat
     1081 aaaatgaaac ataacatgaa tttttgatta gaggagggac aatatgtcga aaaaagaatg
     1141 gttggctggc atgaaagcca tctatccggt ttgtttcggc tacattccga tgggcctggc
     1201 ttgcggagtt ttgctgcagc aagccggcta tcattggctg gcggtcatgc tgatgagcct
     1261 gctcatctac gggggcgctt cccagttcat gatcgcttcg atgacagtgg cgggggcagg
     1321 gctactggaa atggtgatca tgatcttttt catcaatctg aggcacttgt tgatgagttc
     1381 cagcctggct caccggatcc gcgagaagag cacggctttc agcatattct ttgcgcagac
     1441 gatcactgat gaatcgttcg ccgtcaacac gttgtatttc aaaaccgatg gcatgtggaa
     1501 tccgnnnnnn nnnnctacat ccgctatcct tagagcccca agggctccag aacagcgtaa
     1561 ttgattcttt caaaaccgat ggcatgtgga atccgacgaa agcaatggcc gcgaatgtga
     1621 cggcctattc aacttggatt attagcacgg gaataggcgc gatggccgga aagagcatcg
     1681 ctttgtcgcc ggttgtgatg aactacatcc tgattgcgat gttcatcttt ctgctcattt
     1741 cccaaatcga gaaccgtatc gtgttctgga cggcggtatt ttccggcatc acggcggtcg
     1801 tgctgatgaa cttgctgcgc cataacatcg ccatcgtcct gacttcgata ttggcttctg
     1861 ggttcgggct ctatctgcag aacaaaaaag acgataagga ggggattttg cgtggatgac
     1921 aaaacattgt ggctcatttt gggtggcgga ttggtgactt ttttgcccag atggctccct
     1981 atcgttgctt tgaagaacgc gcggattccg atctggttcc agaaatggat gagctttctg
     2041 ccggtttcca tcttttcggc attgatcgct tcggatatct ttttctggga gggaaatgtt
     2101 tccttgaata tattggataa tgccaagct
//
LOCUS       DAOMUJ010000320         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14922_length_2109_cov_1.893379, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000320 DAOMUJ010000000
VERSION     DAOMUJ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_2109_cov_1.893379"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..631
                     /locus_tag="PLT73_07600"
                     /pseudo
     CDS             176..631
                     /locus_tag="PLT73_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
     gene            606..836
                     /locus_tag="PLT73_07605"
     CDS             606..836
                     /locus_tag="PLT73_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HRM20279.1"
                     /translation="MARQPSSYPYNGKEGQPAYVAVDGVVYDVTNVEAWKDGEHKNGL
                     TAGNELTEEITNQSPHGIKVLEGLPIVGELVE"
     gene            1207..>2109
                     /locus_tag="PLT73_07610"
     CDS             1207..>2109
                     /locus_tag="PLT73_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM20280.1"
                     /translation="MRVDLTTKPYNLDAEGIKWVKDTIAGMSVEEKIGQLFINMGSER
                     TEEYLTGVLNNYHIGGVRYNPGMAEEVYEQNKILQENSKIPLLIAANTEAGGNGACKD
                     GTYVGVEVKIAATNDTKYAYEMGRVSGVEAAAIGCNWSFAPIVDINRNWRNPIIATRT
                     WSGDVDKTIAMSLAYMKGIQESGIAPAAKHFPGDGIDERDQHLSFSVNPYSTEEWDAT
                     FGKVYGTLFDAGLPSIMAGHISLPEYVKHFNPEATVQEQILPATLNKYILTDLLRDKM
                     GFNGLVVTDASHMVAMTSAMKRQDM"
BASE COUNT          581 a          467 c          527 g          534 t
ORIGIN      
        1 gcagtgttcc gcggcggaaa tacttgccga agcacaccgg aagaacagca cttttgttgt
       61 cccagatgcg ataataaagg tataggattt caggcgtaga taccgatttg cgtagactac
      121 aggccgagca aatgagtgat tggatgtttc tgggggatta agcggtagtg aataaatgga
      181 aacactatta gcaggaatta catccattac gattggccaa atcgcgatga tgcttatcgg
      241 ggctgtcctg atatatttag gaatcaaaaa agagtatgaa cctacattac ttgtgcctat
      301 gggactgggt accatcttgg tcaacttccc tgggacaggt gttctgaccc aaatggtcaa
      361 cggatctgaa tcagagggtg ttttggacgt tctcttcaaa gcaggtatca gcacggaact
      421 gttcccattg ctgattttca tcggtatcgg agccatgatc gatttcgggc cgctgttgca
      481 aaatccattc atgttattat tcggtgcagc cgctcaattc ggtatcttct ttacgatcgt
      541 agtcgctatc ttcttcggat tcgatatcag agaagctgct tcaatcggta tcatcggtgc
      601 tgctgatggc ccgacagcca tcttcgtatc catacaacgg taaagaaggc cagccggctt
      661 atgtagccgt cgatggagtc gtctatgacg tcactaatgt ggaagcttgg aaagacggag
      721 aacacaaaaa tggattgacg gccggcaatg aactgacgga agaaatcacg aaccagtcgc
      781 ctcatggcat aaaggtcctg gaaggtctgc cgatagtcgg cgagcttgtc gaatgacttt
      841 ccgataatga agataaaatg gggccttcct cgggagagct ccatttttcg attgagtgga
      901 tgctgtgaca aaaaaatgac aatcgcatca ggtttatttt ccggccatca ggatttttcg
      961 gagccttttc tgatttaaag aaatgtcttc atatagcggt ttctgaggcc tagtagggct
     1021 tgccattaac tgtttttgtc caatggttca attttgaaca gaatccgaac tttttgaaaa
     1081 cggggtgtaa gtgccttcat ttttttggat aaaatgccta tacttgttct tgtaggaaag
     1141 acgatgctgg cgccaacgcg ctgcagtatc gatctgttcc agaactattt tgagagggga
     1201 caaatcatga gagtcgattt aactactaaa ccttataact tggatgccga gggaatcaag
     1261 tgggtcaaag atacgattgc aggcatgtct gtcgaagaaa aaatcgggca actgttcatt
     1321 aatatgggtt cagaacgcac ggaggaatat ttgactggcg tattgaacaa ctatcacatc
     1381 ggcggagtcc gctacaatcc tggcatggca gaagaagtat atgagcaaaa caaaattctg
     1441 caggaaaaca gcaaaatccc gttattgatc gctgccaaca ccgaagcagg cggcaacggc
     1501 gcatgtaaag acggtacgta tgtcggcgtt gaagtgaaaa tcgctgccac gaacgacacg
     1561 aagtacgcct atgaaatggg ccgcgtttcc ggtgtcgaag ctgctgctat cggctgcaac
     1621 tggagtttcg ccccgatcgt tgacatcaac cgcaactggc gcaacccgat catcgcaacc
     1681 cgtacatgga gcggcgatgt cgacaaaacc atcgcaatgt cgttggcata catgaagggt
     1741 atccaggaaa gcggcatcgc accagcggca aaacacttcc ctggggacgg aatcgacgaa
     1801 cgcgaccaac atttgtcatt cagcgtaaac ccatattcca ctgaagaatg ggatgctact
     1861 ttcggtaaag tgtacggcac tttgttcgat gctggtttgc cttccatcat ggcaggtcac
     1921 atctcattgc ctgaatacgt gaaacacttc aaccctgaag caactgttca agaacaaatc
     1981 ttgccggcta ctttgaacaa atacatcctg actgacttat tgcgcgacaa aatgggcttc
     2041 aacggactgg ttgttaccga tgccagccac atggttgcga tgacttccgc catgaaacgc
     2101 caagatatg
//
LOCUS       DAOMUJ010000321         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14981_length_2102_cov_2.722521, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000321 DAOMUJ010000000
VERSION     DAOMUJ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14981_length_2102_cov_2.722521"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..718)
                     /locus_tag="PLT73_07615"
     CDS             complement(362..718)
                     /locus_tag="PLT73_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20281.1"
                     /translation="MNKTRIMTILSVAGLFVGGAAPVLAASDTAAQNGTMTQEQIKSG
                     ECTNEGAKKQGNEEATMKRAREKSGTCDGTGTQANQDGNQNATGEADGDGNQNGTAET
                     DNDGKNGVRDQSCQTE"
     gene            885..1586
                     /locus_tag="PLT73_07620"
     CDS             885..1586
                     /locus_tag="PLT73_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM20282.1"
                     /translation="MVKLLIADDNKQITSILKEYSEKDGYEAVVAYDGEQALQEFGKD
                     KFDLILLDVMMPKKDGFEVCREIRKVSSIPIIMVTARGEDFERIMGLDIGADDYIVKP
                     FSPGEVMARIRAILRRLDQTSDNKDHHKLYVHENLEIRLDEFIVKVAGHQVHLTKKEI
                     ELLWILATNKNKVFSRDNLLDSVWGYDYFGDNRTVDSHIKRLRAKVDQIPHPSWDIKT
                     VWGIGYKFEVNPDEV"
     gene            1576..>2102
                     /locus_tag="PLT73_07625"
     CDS             1576..>2102
                     /locus_tag="PLT73_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20283.1"
                     /translation="MKYKITRKLVLYFTSVILLFSLVVGGAFLLLFTRHTEQIYRADM
                     QERAEAIAAGVADYLEDGSTALDEVPVIAKGSANGKQTGTALLVQSGATGTTGRNNTS
                     GYNTYITAIQDIAMGEVWIVDQSAETISVGTGKKAITYAELPSAALALVDEVFEGDVA
                     FNEGFSPLLEDTSITV"
BASE COUNT          486 a          496 c          574 g          546 t
ORIGIN      
        1 gcgtacatgg cgctgcttgc ggtcatgagt tatgggcgaa gcattggata ggttgaggag
       61 tttttgcatg gagaagttgt ggggctaccc agctccggtt aaggaggctt cgccggagtt
      121 gacaccacat tcagatgccg tcttccgacc agctgaagct acatcggaga agagcccatc
      181 tatttaccgg ctcctccggc gaacgaaagc ctggccggag ctggcgggta tgtttttgca
      241 gtcttgttct ctcgatctgt gggatttcag ctgcaaaagt ggaatacgca caaaaagggg
      301 cgatctcatt gcgagaccgc cccttcttgt cttatagtct tcactctaat gtgatggact
      361 gttattcagt ttggcaagat tgatcgcgga cgccgttttt gccgtcattg tccgtttcag
      421 ccgtaccgtt ttgattgccg tcgccgtctg cttcgcccgt agcgttttgg ttaccatcct
      481 gattggcttg cgtacccgtt ccatcgcacg tgccgctttt ctcgcgggca cgtttcatgg
      541 ttgcctcttc attgccttgt ttttttgctc cttcatttgt gcattcgcct gacttgattt
      601 gctcttgggt catcgtgccg ttctgggctg ccgtgtcgct cgctgccagt accggtgctg
      661 cacctccaac aaataagcct gcaacggata ggatggtcat gattcttgtc ttgttcattt
      721 ttgtttcccc ctggtatgat tttttgttat ttcttactga cctaagaata atcaaaaagt
      781 atggcagaag cgtgttttta aaggcacatt tgtgtggtat tatcggggtg aaaacataat
      841 cttgccatat ttttgcagta tgatttcccc gaggagggag tacgatggta aagctattga
      901 ttgcggatga caacaaacaa ataacatcca tcctgaagga gtattcagaa aaggatggct
      961 acgaggcggt cgtcgcctat gatggcgaac aggcgctgca ggaattcggg aaagacaaat
     1021 tcgatttgat tttgctggat gtgatgatgc cgaaaaagga tggcttcgaa gtgtgcaggg
     1081 aaatccggaa agtttcctcg attcccatca tcatggtgac cgccagaggg gaagattttg
     1141 aacggatcat gggcttggat atcggagcgg acgactacat cgtcaagcct ttttcgccgg
     1201 gtgaagtgat ggcgcgcatt cgggcaatcc tacggcggtt ggatcagaca tcggacaaca
     1261 aggatcacca taaactctac gtccatgaaa atctggagat ccgattggac gaattcatcg
     1321 taaaggtcgc cgggcaccaa gtccatctca cgaaaaaaga gatcgagctg ctttggattt
     1381 tggcgacaaa caagaacaag gtgttcagcc gcgacaatct gttggatagt gtctggggct
     1441 acgactattt cggcgataat cggacggtcg actcgcacat caagcgcctg cgggcaaagg
     1501 tcgatcaaat tccccatccg tcgtgggaca tcaaaaccgt ttggggcatc ggctacaaat
     1561 ttgaggtgaa cccagatgaa gtataagatc acccgcaaac tggtcctcta tttcacttcc
     1621 gtcatcttac tgttttcctt ggttgtcgga ggggccttcc tgctgctgtt cacccggcat
     1681 accgaacaaa tttaccgggc ggatatgcag gaacgggctg aggcgattgc tgccggcgtg
     1741 gcggattatt tggaagacgg gagtaccgct ttagatgagg ttccggtcat tgcaaagggc
     1801 agtgccaacg gtaaacaaac aggaactgcc ctgcttgtac aaagtggtgc gacggggaca
     1861 accgggcgca acaatacgag cggatacaac acctatatca cggccattca ggatatcgcg
     1921 atgggcgagg tatggatcgt cgaccaatcc gcagagacaa tcagtgtcgg aaccggcaaa
     1981 aaagcgatca cctatgccga actaccaagc gctgcgcttg cgcttgtcga tgaggtattc
     2041 gaaggggacg tcgccttcaa cgaaggcttc agcccgttgt tggaggatac ctccatcacg
     2101 gt
//
LOCUS       DAOMUJ010000322         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_14993_length_2101_cov_1.873412, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000322 DAOMUJ010000000
VERSION     DAOMUJ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14993_length_2101_cov_1.873412"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..333
                     /locus_tag="PLT73_07630"
     CDS             13..333
                     /locus_tag="PLT73_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HRM20284.1"
                     /translation="MSLEVTKRQGIVVWVYTLRQIKNLKRYGYIHYVSNRMKYVLLYV
                     DQEEAQTTAEKLNSLHFVRKVEVSHRPEIDMTLKNALPGRKDRNNPEVEYHESAFETK
                     TYSF"
     gene            376..942
                     /gene="rsmD"
                     /locus_tag="PLT73_07635"
     CDS             376..942
                     /gene="rsmD"
                     /locus_tag="PLT73_07635"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRM20285.1"
                     /translation="MRVIAGEYKGRRLKSVPGSNTRPTTDKVKESLFNIIGPFFEGGV
                     SLDMFAGSGGLSIEAVSRGIDRAVLFEKNRKALDTIKENIAITKEAEKFTIYAGDARK
                     NLRVFAVENPNIQFALVFLDPPYADEKTVADITEMIALQLVNEETVFVCEMAKENTLP
                     EQIGPYGKWKRAVYGTIAIEMFDYNQEG"
     gene            947..1462
                     /gene="coaD"
                     /locus_tag="PLT73_07640"
     CDS             947..1462
                     /gene="coaD"
                     /locus_tag="PLT73_07640"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRM20286.1"
                     /translation="MKKTALFAGSFDPLTNGHIDTISRAAIVFDEIVVAVSTNTSKQS
                     LFDGEERIRLVAEALRDFPQVRVVRHTGGLTIEMARDVGACALLRGVRNVKDFEYEHS
                     IASMNKLQAPDLETVILLSSENYRYLSSSLIKEVAMFGGDVSTLVPANINAAILQKFQ
                     DSAFKGKKISE"
     gene            1614..>2101
                     /locus_tag="PLT73_07645"
     CDS             1614..>2101
                     /locus_tag="PLT73_07645"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRM20287.1"
                     /translation="MDAIREWLACHKQEVIRSAVLFMFIALCLFGLGRLIYGSTAGTD
                     TGELLLDETYLAVSAAESNQSPEQPEESEPVQEIIIVDIKGAVKNPGVYRAETNMRVI
                     DIIDLAGGLSETADADTVNLSQRVADQMVIYIPAVGEEADRPITVAENPLAEQESGNG
                     VAG"
BASE COUNT          561 a          430 c          592 g          518 t
ORIGIN      
        1 gggggtaaac atatgagctt agaagtgacc aaaaggcaag gcatcgtcgt ctgggtctat
       61 accttgagac aaatcaaaaa tttaaagaga tacggttata tccattatgt ttccaacaga
      121 atgaagtatg tcctgttgta tgtcgaccaa gaagaggcgc aaaccacggc cgaaaaattg
      181 aacagtctgc attttgtccg gaaagtggaa gtgtcgcatc gtcctgaaat cgacatgacc
      241 ctgaagaatg ctcttcctgg caggaaagat cgtaataatc ctgaagtgga ataccacgag
      301 tcggcttttg aaacaaagac ttattctttt tagtggtgat cggaaataaa aaatactttt
      361 tgtaggtgag tgcagatgcg ggttatcgca ggagaatata aaggacgcag attaaaaagc
      421 gttcctggca gcaatacgcg gccaacaacg gataaggtca aagagtcctt gttcaacatc
      481 atcggtccgt tttttgaagg cggagtcagc ttggacatgt tcgcgggcag cgggggctta
      541 tcgatcgaag cagtatcccg cgggatcgat cgagcggtgt tgttcgaaaa gaaccgaaaa
      601 gcacttgata cgatcaagga aaatatcgca atcacgaaag aagcggagaa attcactatt
      661 tatgcaggtg atgcgcggaa aaatctgcgt gtcttcgcgg tggaaaatcc gaatatacaa
      721 tttgcgctgg tctttcttga tccgccgtat gctgatgaaa agacagttgc tgacatcact
      781 gaaatgatcg ccttgcaatt ggtcaatgaa gagaccgtat ttgtctgtga aatggcaaaa
      841 gaaaatacgt tgcctgaaca gatcggcccg tacggaaaat ggaagcgtgc ggtttatggg
      901 accatcgcta tcgaaatgtt cgactacaat caggaagggt gatcccatga aaaaaacagc
      961 gctatttgcg gggagttttg accctttgac gaacgggcac atcgatacga tttcgcgtgc
     1021 agcgattgtt ttcgacgaaa tcgttgttgc agtatcgacc aacacatcca agcagtcttt
     1081 atttgacggt gaggaaagaa tccggttggt cgccgaagct ttgcgtgact ttccgcaggt
     1141 ccgcgtcgtc cgccataccg gggggcttac cattgaaatg gcaagggatg tcggcgcctg
     1201 cgcgcttttg cgaggggtgc gaaacgtgaa ggatttcgag tatgagcaca gcattgcctc
     1261 gatgaacaag ttgcaggccc cagatctgga gacggtgatt ctgttgtctt cggaaaacta
     1321 tcgctacctc agttccagcc tgatcaagga agttgcgatg ttcggcggag atgtatccac
     1381 cttggtgccg gccaacatca acgccgctat ccttcagaaa ttccaggaca gcgcgttcaa
     1441 gggtaaaaaa atatcggagt gaatcgagta tttgcataag gttggaagag gagccgttgg
     1501 gttcttttcc caaccttttt gttttggtga gcagatggat catttttttg gacggctgaa
     1561 tgtttctgcg tcttttcgcg tatgttgtca atggatgcga aaggagggat agtttggatg
     1621 ctattcggga atggcttgct tgccataaac aggaagtgat ccggtctgcc gttttgttta
     1681 tgtttatcgc cctatgtctc tttggcttgg gcagattgat atacggcagc acggcaggaa
     1741 ccgacacagg ggagctgctg ttggatgaaa cctacctggc ggtaagcgct gctgagtcta
     1801 atcagtcgcc agagcagccg gaagagtcgg agccggtcca ggaaattatc atcgtcgaca
     1861 tcaaaggggc cgtaaaaaat ccgggtgtat accgggctga gaccaacatg cgcgtcattg
     1921 acatcattga tttggcgggt ggcttgagtg aaacggctga tgccgacaca gtgaatctat
     1981 cacagcgtgt tgcggatcag atggtcatct atattcctgc tgtcggggaa gaagcggatc
     2041 ggccgattac ggtggcggaa aatccgctgg ccgaacaaga gagcggaaat ggggtggctg
     2101 g
//
LOCUS       DAOMUJ010000323         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15151_length_2083_cov_2.099112, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000323 DAOMUJ010000000
VERSION     DAOMUJ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15151_length_2083_cov_2.099112"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLT73_07650"
     CDS             complement(<1..270)
                     /locus_tag="PLT73_07650"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRM20288.1"
                     /translation="MFKMLFENVQQQTPLVHCITNYVTVNDVANALLAAGGSPIMADA
                     PEEVADITSICTALNLNIGTLNSRAVESMLLAGRQANALAHPVVLD"
     regulatory      complement(344..450)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(514..2061)
                     /locus_tag="PLT73_07655"
     CDS             complement(514..2061)
                     /locus_tag="PLT73_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM20289.1"
                     /translation="MRRKTFSTIFTLGITAGLLASCGSGESADQQDSAQQESVQSADA
                     YAGVVKTSIYVKGYEGGPGVPKLIIELEDAVDSVDPSGWQVVTAGAERTVTDVYTSDA
                     NGEPQENSNYIAIDMETGFNDETFSVVGSPFTYNTTTSMNEWSPAYEVTIAAPSITIE
                     DEDYSLSITEDAVNNRISPDTDLFAARGTFSGSYVNPMTDQAEELTLSTAAYEPADLS
                     EGEANPLVIWLHGQGEGGTDPDIAILGNEVSALAKEEIQSYFHTDDVTGAYVLAVQTP
                     TYWMDEGDGTNGNGSGISRYTEILMDTINDYVAAHPDVDTDRIYLGGCSNGGYMTMNM
                     VIHYPDYFAAAYPICEAYAYYEYERNSDGTYVKASEEATGTSAFVKTGRVWFTEEKLQ
                     AIKDLPIWFVQSADDPVVVPANYMLPTYQALVKAGAENTWMSYFETVEGTDSPGTTYL
                     GHFSWIYLFNNQVTGVQDKEAIAAAADTETFGSEPSNAAGGGTAAATVGGTTYANIFE
                     WLNAQSR"
BASE COUNT          462 a          573 c          502 g          546 t
ORIGIN      
        1 atccaaaaca acgggatggg cgagcgcatt ggcttggcgc ccggccagca gcatggattc
       61 cacggctcga ctgttcagtg taccgatatt cagattcaag gcggtgcaga tgcttgtgat
      121 gtccgcgact tcctcggggg catccgccat gatcggcgac ccaccggcag ccaagagggc
      181 attagcgaca tcattgacgg tgacgtaatt ggtgatgcag tggaccagcg gcgtctgttg
      241 ctggacattt tcgaatagca ttttgaacat gaggtacctc caaatagttt ttttgcgaca
      301 aaaaaagcga gaaatgtccg gataggcatt tcccgctgaa tagaatcatt caatgttatt
      361 tccctacgtt ggcattatcc aaatcaggta atcggtcgaa gttgattact tcctctcagc
      421 ctgctgacac aagctccctt tttcgtatgt aattttgtaa ctatcttaac ataagcgcca
      481 gccttactca agaatcattt ttgctgattc tttttatctt gattgcgcat tcagccactc
      541 gaaaatattc gcataagtgg ttccgccgac tgtggctgca gccgttcctc cgccggcagc
      601 gttgcttggt tctgatccga atgtttcagt gtcggcagca gccgcgatcg cttccttatc
      661 ctggacgcct gtcacctggt tattgaataa gtagatccag gagaaatggc ccaggtaagt
      721 ggtgcccggg ctgtccgttc cttcaaccgt ttcaaagtag gacatccaag tattttccgc
      781 tcctgcctta acaagtgcct gataggtagg cagcatgtag ttcgcaggga cgacaaccgg
      841 gtcatcagct gattggacaa accaaatcgg caagtctttg attgcctgga gtttttcttc
      901 cgtgaaccaa actcgcccgg tcttcacgaa agcggaagta ccggtagctt cctcgcttgc
      961 tttcacatac gtaccatcac tgttgcgctc gtattcataa taagcgtacg cctcgcagat
     1021 tggataagct gcagcaaaat agtcagggta atggatgacc atgttcatgg tcatgtagcc
     1081 accgttcgag cagccgccca gatagatgcg atcggtatcg acatccggat gcgcagctac
     1141 ataatcattg atggtatcca tcaatatttc ggtgtatctg gagatgccgc tgccgttgcc
     1201 gttcgttccg tccccttcgt ccatccaata ggtaggcgtt tggacagcca gaacataagc
     1261 cccggtcaca tcatcggtat ggaaatagga ctggatctcc tcttttgcca acgccgatac
     1321 ttcatttccg aggatggcga tgtccggatc cgttccgcct tcaccctgac cgtgcagcca
     1381 gatgaccaac ggattcgctt ccccttcgga aaggtccgcc ggttcatagg cggctgtcga
     1441 aagcgtcagt tcctccgcct gatccgtcat cggattcacg tagctgccgc tgaaagtccc
     1501 tctggcggca aacaaatcgg tatccggtga gatgcggttg ttcacggcat cctccgtgat
     1561 gctcaaagag tagtcctcat cctcgatcgt gatcgaaggc gcagcgattg taacctcata
     1621 agctggggac cactcattca tcgatgttgt cgtattgtag gtgaacgggg atccgaccac
     1681 actgaacgtc tcatcattaa aaccggtctc catatcgatt gcgatatagt ttgaattctc
     1741 ctgaggttcc ccgtttgcat cggaagtgta gacatccgtc accgtgcgct ccgccccggc
     1801 cgtcacgacc tgccagccgc tgggatcaac actgtcgacc gcatcctcca attcgatgat
     1861 aagcttcggt acgcccggtc ccccttcata tcccttcaca tagatgctgg tctttacgac
     1921 gccagcatag gcatcggccg attgcacgct ttcttgctgg gctgaatcct gttgatccgc
     1981 cgattctccg gagccgcaac tggccaataa gcctgccgtt atccctaatg taaaaatagt
     2041 gctgaatgtc ttacgtctca ttctctaatc cctccatatc cct
//
LOCUS       DAOMUJ010000324         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15189_length_2079_cov_1.935771, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000324 DAOMUJ010000000
VERSION     DAOMUJ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15189_length_2079_cov_1.935771"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..728
                     /locus_tag="PLT73_07660"
     CDS             51..728
                     /locus_tag="PLT73_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003137744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20290.1"
                     /translation="MKLIEMIDVSRSFGEGELKIDALKKTNIAIEAGEFVAVIGPSGS
                     GKSTFLTITGGLQTPTSGKVLINGNPFSEVNEKKRAKLRFEEIGFILQASNLVPFLTV
                     QDQLHLANKVEGSKVDKQKRDELLKELGIFELKNKFPSDLSGGQRQRVAIARALYHDP
                     SVILADEPTASLDTQKAFEVVEILARETKLKKKATIMVTHDERLIDYCDKVYVMKDGI
                     LSERIGG"
     gene            756..1154
                     /locus_tag="PLT73_07665"
     CDS             756..1154
                     /locus_tag="PLT73_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRM20291.1"
                     /translation="MDSIKLYFLTFFVFFLVDIVWLGVLSKNIYSKYLGHLMASNVNW
                     VAAIIFYFLFIGGLVFFVIHPALLKESLQFAILAGGFFGLIAYATYDLTNLATIKDWP
                     VIITVIDLAWGTFLNAATAGITYIAAQRFL"
     gene            1194..>2079
                     /locus_tag="PLT73_07670"
     CDS             1194..>2079
                     /locus_tag="PLT73_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM20292.1"
                     /translation="MNISTLDSERLYNSFLSGAHEVIKNKNSLNEINVFPVADGDTGS
                     NLSSTMHAIIMDAKISGSVKETMNSIADAALTGARGNSGIIIAQYINGIFLSLMDDEM
                     ITISSFAETVKNAVPYAYQAISNPVEGTIITVIREWADAVYSHKDLSVTFSELFSKSL
                     ITANKSLEATTSRLKVLQDSKVVDSGAKGFVYFLQGFATFLHTGKLDLKVSEEQEDLS
                     FNEELIHGQGEIRHRYCTEALLSADSIDLEALRSEMALYGDSLIVAGNNHKARIHIHA
                     NAPEQVFQVLRKKDIILQQ"
BASE COUNT          631 a          392 c          491 g          565 t
ORIGIN      
        1 cggttgtaaa aatagaccca ttaaaggcaa ttggatagga gagacagcag atgaaattaa
       61 tagaaatgat agatgtttcc cgatcattcg gtgaagggga attaaagata gatgcgctga
      121 aaaaaacgaa catagccatc gaagccggtg aatttgttgc agtcattggc ccgagtgggt
      181 caggaaagag tacttttttg actattacag gagggctgca gaccccaact tccggtaagg
      241 tcttgataaa tgggaatcca ttcagtgaag taaacgagaa aaaacgagcc aaacttagat
      301 ttgaagaaat cggttttatt ctgcaagcct caaacctagt cccgtttttg acggtccaag
      361 atcagctgca cttggccaat aaagttgaag gcagtaaagt tgataaacaa aaacgcgatg
      421 aattactaaa agaattaggc atattcgaat tgaagaataa gtttccgagt gatctctccg
      481 gcggtcaaag gcaaagggtc gcaatagcga gagccctata tcatgatccg tctgtcatcc
      541 ttgccgatga accaacggcc agtctggaca ctcagaaagc atttgaagtt gtcgaaattc
      601 ttgcccgaga aacaaaatta aagaaaaagg cgaccattat ggttacacat gatgaacgtt
      661 tgatagatta ttgtgacaaa gtatatgtaa tgaaagatgg catcttgtcc gaaagaatcg
      721 gcggataaac aaataacgga atggaggaag agaagatgga cagcatcaaa ttgtatttcc
      781 tgacattttt tgtgttcttt ttagtggata tagtctggtt aggagtattg tcgaaaaata
      841 tttacagcaa gtacttgggg cacctcatgg cttcgaatgt taattgggtg gcagcgataa
      901 tattctattt cttgttcatc ggtggactcg tattttttgt gatccatccg gcacttttga
      961 aagaaagtct acagtttgcc attttggcag gtggcttttt cggactgatc gcttatgcga
     1021 cgtacgactt gactaatctg gctaccataa aagattggcc ggttattatc accgtaatcg
     1081 acttggcctg gggaactttt ttgaatgccg cgacagctgg gatcacttac attgcggcgc
     1141 aaagatttct ctaaaaaatg aataaagttt tagatgtgga cggaggaaga acgatgaata
     1201 tttcaaccct tgatagcgag aggctttaca attctttttt atcgggtgcg catgaagtca
     1261 taaagaacaa gaacagtctg aacgaaatca atgtatttcc ggtcgctgat ggagatactg
     1321 gcagcaacct ttcctcaacc atgcatgcga tcatcatgga cgcaaaaatc tcgggatccg
     1381 tcaaggaaac gatgaacagt atagctgatg ccgctttgac tggtgcaaga gggaactcgg
     1441 gcatcattat tgcccaatac atcaacggga tttttctcag tctgatggat gacgagatga
     1501 taaccatttc ttcatttgca gaaacggtca aaaatgcggt cccttatgcc tatcaggcta
     1561 tttccaatcc ggttgaagga acgatcatca ctgtcattcg tgaatgggct gatgctgtat
     1621 atagccataa agatctttcg gtaacgtttt cagaattgtt ttcaaagtcg ttgattacag
     1681 ccaacaaatc tttggaggcg acaacttcac gtttgaaagt gctccaggat tccaaagtcg
     1741 tcgattccgg tgcgaaagga tttgtctact ttctgcaagg atttgcaaca tttttgcaca
     1801 ccggaaagtt ggatttgaaa gtcagcgaag aacaggaaga tctttctttt aatgaagaat
     1861 tgatccacgg ccaaggggaa attcgccatc gctattgtac tgaagcgcta ttgtccgcgg
     1921 actctatcga tctggaagcg ttgaggtcgg aaatggcact ctatggcgat tctttgattg
     1981 tggcaggcaa caatcataaa gccaggatcc atatccatgc caatgctccg gagcaagtgt
     2041 tccaagtttt gcgcaagaaa gacatcatct tgcagcaaa
//
LOCUS       DAOMUJ010000325         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15242_length_2073_cov_1.537661, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000325 DAOMUJ010000000
VERSION     DAOMUJ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15242_length_2073_cov_1.537661"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PLT73_07675"
     CDS             <1..1332
                     /locus_tag="PLT73_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20293.1"
                     /translation="PVDYPFGRPIRSGRMRAGALLDFLPEKELNGDEEIIGKKVFGMA
                     DNHKKKKRILGVESNIFFVGLTSFLTDTTTKMVYSILPLFLTTLGASKTEISLIEGIA
                     ESTASVLKSLSGWWSDKIGRNKPFMIIGYGITALLSPLFAFVTTPLQVLSIRFVERVG
                     KGIRTAPRDSLVAGSSEENNNAGLNFGFHKAMDNSGAIVGPLIAFAVLAAFPGDYRRV
                     FLFAAIPGFLGLLSILFFVKEAKRTKSERLGKISFRDFPKDFYLFLAIVFLFTLGNST
                     DALLLIKASDVGVSDAFIPIMYLVFNAVSVAFAVPLGKLSDKIGRKRIIIGGYLMFAL
                     IYFGFGKAATPFAIVGLFAFYGLYSAATDGIQKALVSDIIGKEKKGTGLGLYNALLGM
                     TLLPASLIGGWLYDTFNNQVPFYFGSATALLSAFLMFLFYQRGKRHGTSHS"
     gene            complement(1597..1806)
                     /locus_tag="PLT73_07680"
     CDS             complement(1597..1806)
                     /locus_tag="PLT73_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HRM20294.1"
                     /translation="MADITFEIVEELGVLSTSAKGWTKELNLVSWNGRPPKYDIREWS
                     PEHEKMGKGLTFSEEEMAALAKLLK"
BASE COUNT          445 a          532 c          524 g          572 t
ORIGIN      
        1 ccggttgatt atccatttgg cagacccatc cgaagcggac gtatgcgcgc gggcgctctg
       61 ctagactttt tgccggaaaa ggaattaaat ggagatgaag aaataatcgg aaagaaggtt
      121 ttcgggatgg cggataatca caaaaagaaa aagcggatcc tcggcgtcga aagcaatatt
      181 ttctttgtcg gcttgaccag tttcctgacg gatacgacga caaaaatggt ctacagcata
      241 ctgcctttat ttctgacgac actgggcgcc tcgaaaacgg aaatctccct gattgaaggg
      301 atcgccgaaa gcaccgcttc cgtcctgaaa tccctctccg gttggtggag cgataaaatc
      361 ggacgcaaca agcctttcat gatcatcggt tacggcatca ccgcgctcct ttccccgctg
      421 ttcgcattcg tgacgacacc cttgcaggtc ctgtcgatcc gttttgtcga acgcgtcggc
      481 aaaggcatcc gcaccgctcc ccgggacagt cttgtggccg gttcttctga agaaaacaac
      541 aacgccggtc tgaatttcgg tttccataaa gcgatggaca acagcggagc catcgtcggg
      601 ccgctgatcg ccttcgctgt actggccgct tttcctggcg attaccgacg cgtcttcctg
      661 tttgcggcca ttccgggatt tttgggactg ctgtcgatcc tcttttttgt gaaggaagcc
      721 aaacgcacca aatcggaacg gctaggaaaa atatccttcc gggattttcc caaggatttc
      781 tacctttttc tggcaatcgt tttccttttc acgctgggga attcaacgga tgccctgctt
      841 ttgatcaaag cgagcgacgt cggcgtcagc gatgccttca tcccgatcat gtatctggtg
      901 ttcaatgcgg tttccgtcgc cttcgccgtt ccgctcggga aactgtccga taagattgga
      961 cgtaaacgca tcatcatcgg cggctatctg atgttcgcgc tgatctattt tggattcggc
     1021 aaggcggcta cccctttcgc catcgtcggc ctcttcgcct tttacggcct ctacagcgcg
     1081 gcgacggacg gcatccagaa agcgttggta tccgacatca tcggcaagga gaaaaaaggc
     1141 accggattgg ggctatacaa tgcgctcctg ggaatgaccc tattgccggc cagtctgatc
     1201 ggcggatggt tatacgatac tttcaataac caagtacctt tctacttcgg ttccgccacc
     1261 gccttgcttt ccgctttttt gatgttcctc ttctatcaac gcgggaaacg ccacggcaca
     1321 agccattctt gatgcaatgc tgcattcttc taagggatag ggttagcggc ggaattcccc
     1381 gtcaaactgg ttttgacggg gaatcttgtg tcgtgtgtgg ttgtacttcc ccgccaagac
     1441 gattttggag gggaaactga ataggattcg aattgatttt gcccgccaat aaggttttgg
     1501 cgggttattt ctaaggataa cgtatattca acctcaaaac tgaagaggct gcatcaatcc
     1561 agtaagtagc gttctgggtt gatgcagcct cttcggttat ttcaatagtt tggcgagggc
     1621 agccatttct tcttcgctga aggtcagccc tttgcccatc ttttcgtgtt ccggactcca
     1681 ttcgcggatg tcgtattttg gcggtctgcc gttccagctg accaagttca attctttcgt
     1741 ccaacctttt gcggatgtgg acaggacgcc gagttcttcg acgatttcaa atgtgatgtc
     1801 tgccataggg aggatccttt ctattttgga gtttgaggtt cgctgcctgg acgcttttcg
     1861 gatcggcagc ttgcctcttt attatagcgc aaattggggg tcatttaaaa tatccgcgga
     1921 ctttcgaaat atgtgttttc tctttcagtt gccgaataaa tgctgtcagg ctttccatcg
     1981 gtccagcacc ttgatcgcta aagccgagaa gtaatcagcg gcgacagcca agccttcctc
     2041 cttgagattg aactcaggat ggtgccattc gtg
//
LOCUS       DAOMUJ010000326         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15271_length_2070_cov_2.537469, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000326 DAOMUJ010000000
VERSION     DAOMUJ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15271_length_2070_cov_2.537469"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..209)
                     /locus_tag="PLT73_07685"
     tRNA            complement(120..209)
                     /locus_tag="PLT73_07685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Ser,seq:cga)
     gene            427..1647
                     /gene="sstT"
                     /locus_tag="PLT73_07690"
     CDS             427..1647
                     /gene="sstT"
                     /locus_tag="PLT73_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HRM20295.1"
                     /translation="MYKLMNHWYKLSLVKRIIAGLVIGIILAVTIPDIAKPITIFGAL
                     FVGALKAIAPILVFFLVASAISQHKKGQQTNMKSVIVLYLLGTFLAALVAVVASFLFP
                     VTLTLGSGVEDVTAPGGVVEVLKALLLNVVDNPIKALFNANYIGILSWALMLGFTLKN
                     AAASTKLMLSNVSDALTEMVRIVIAFAPLGIMGLVFDVIATSGIGVLLGYGKLLAVLI
                     GCMLFVALVVNPIIVYIYIRSNPFPLVFRCLRESGITAFFTRSSAANIPVNMTLCEDL
                     GLDRDTYSVSIPLGATINMAGAAGTISVLTLAAVHTLGIPVDFGTALILSILAAISAC
                     GASGVAGGSLLLIPLAASLFGIPNDVAMQIVGVGFIIGVLQDSFETALNSSTDVLFTA
                     TAEFAERRKQGIKI"
BASE COUNT          503 a          458 c          502 g          607 t
ORIGIN      
        1 aggtgaggat cactcggccg attttttttt aggtgtaccg gttgatttcg acaaaacaaa
       61 aagccggcac tcccgaatgg agtaccagcc ttttgtcgga caaaatttaa aagtttttaa
      121 ggagaatggg ggattcgaac ccccgcgcgc ctttcgacac ctaacgcatt tcgagtgcgt
      181 cctcttcagc cggacttgag taattctcca taaaatatat tatagcgctt ttcagctgat
      241 cagaaaagag gaattttaaa aaaagtcacg aaaatgcaga aagcaaataa tgagggatcc
      301 aattcaacag acacgcggta agagaaaagt gaaaggtatt cttcctaggg agattaaatt
      361 atggtaatat gaaaacctat gtactcattt tattgtaatt tttgcatata tcaggaggaa
      421 tttgaattgt ataaattaat gaatcactgg tacaagctga gtcttgtcaa acgtatcatc
      481 gctggtttgg tcatcggtat cattcttgct gtcactatac cggacatagc aaaaccgatc
      541 acaatttttg gtgcgttgtt tgtcggagct ttgaaggcaa ttgcacccat cttggtattt
      601 ttcttggtag catctgcgat ttcgcaacat aaaaaaggtc aacagacgaa catgaagtct
      661 gttatcgtgc tgtacctttt aggaacgttc ttggcggcac tcgtagcggt tgtcgcaagc
      721 ttcctcttcc cggtgacttt gacattgggt tccggcgtcg aggatgtcac tgctccaggc
      781 ggtgtggtgg aagtcttgaa agctttgttg ttgaacgttg tggataatcc aatcaaggcg
      841 cttttcaatg ccaactacat cggcatcttg tcttgggctc tcatgctcgg ttttactttg
      901 aagaacgctg cggcttcaac caagttgatg ctgtccaatg tttctgatgc attgacggaa
      961 atggtccgga ttgtcattgc cttcgctccg ttgggaatca tgggtcttgt tttcgatgtc
     1021 atcgctacaa gcggaatcgg cgtattactg ggctatggca aactgcttgc ggtgctgatc
     1081 ggctgtatgt tgttcgtcgc tttggtcgtc aacccgatca tcgtctacat ctatatccgt
     1141 tccaatcctt tcccgctcgt cttcagatgt ttgagagaaa gtggcatcac tgctttcttc
     1201 acgcgaagtt cggctgcgaa cattcctgtc aacatgaccc tttgcgagga ccttggattg
     1261 gacagggaca cgtattcggt ttccattccg cttggtgcaa cgattaatat ggcaggtgcg
     1321 gcggggacga tttccgtcct aaccttggca gcagtccaca ccttgggtat ccctgttgat
     1381 ttcggtactg ccctcatcct cagtatcctg gcagcgatca gtgcttgtgg agcatcgggt
     1441 gttgctggtg ggtcactgtt gcttatcccg ttagcagcaa gtttgttcgg tatcccgaac
     1501 gatgtggcta tgcaaatagt cggtgtcggt tttatcatcg gcgtccttca ggattccttt
     1561 gagacagctc ttaactcatc gactgatgtc cttttcactg cgacagcaga atttgcggag
     1621 cgacgcaagc aaggcatcaa aatctgattc aagaccgtaa agcaaaaaaa ccactgatcc
     1681 ctatgtcggg tcagtgtttt ttttggatct gtagattgat tactatctgg tctggcatca
     1741 atgaggctgt gactatcgaa taccagacag atgtgtgatt tctcggcagt tcgcaacgcc
     1801 atcctagccg tatgcccgac agaagggcaa tctgtcgggt gttttgcggt tttcggtaat
     1861 caatcagtcg acagatgagt gacctctcgg cagtccgcga gatacagaag aaccgaaaca
     1921 cagtacaggg gccgtctcga aacgagacgg cccctgtact gcacagaact ttaaagttgt
     1981 ttactgaaga ttcataccga aatcagtctt ctgtccctaa gatttgattg atttcttggg
     2041 agagcaaatc tgctttaccg ccgaatacag
//
LOCUS       DAOMUJ010000327         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15302_length_2067_cov_1.897614, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000327 DAOMUJ010000000
VERSION     DAOMUJ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15302_length_2067_cov_1.897614"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLT73_07695"
     CDS             complement(<1..345)
                     /locus_tag="PLT73_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM20296.1"
                     /translation="MSNELRRSNSFFDLLSDTGSLFQNRFFQEIKMDVNETEDNYQVT
                     ADLPGYAKEDITVDYENDILSISATRKSEKVDKDEKGQIIRQERSSGSVHREIYLKGI
                     NEEDITATLTEGV"
     gene            561..>1017
                     /locus_tag="PLT73_07700"
     CDS             561..>1017
                     /locus_tag="PLT73_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM20297.1"
                     /translation="MKKIEAGSLYLKKHRTLFQCPICKADFEKVDGHSIICANGHHFD
                     VSKKGTLHLLMKGGQNDYGKEMLSSRKNLADTGFFFPLLDAVYAHIDKPESAAVLDVG
                     CGEGSHLHYLTEKGLKGTKIGFDISKDAIQLAASNFFPDAFWCVADLAHS"
     assembly_gap    1018..1027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1028..1374
                     /locus_tag="PLT73_07705"
     CDS             <1028..1374
                     /locus_tag="PLT73_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HRM20298.1"
                     /translation="QEFDRVLKPGGKVIKVVPNAQYLIELREQLYALDTTKREYDNQQ
                     VVDRFYEIYPDAISEEVAYSVALDAKSYGWLMEMTPLSWGASETALANAAANPLKSVT
                     VAVTLLVASKSV"
     gene            1631..>2067
                     /locus_tag="PLT73_07710"
     CDS             1631..>2067
                     /locus_tag="PLT73_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HRM20299.1"
                     /translation="MDFAALKAEIMEESKKLGIDKIGFTTAEPFEYMLESLKEQHAKG
                     HTVGFEHPVLDERIYPDRIFDNPQSILSIALAYPSKMTEKAERVRGERRGNFSRASWG
                     TDYHVILRERMDRLIAFIQEKMPVARFKPMVDTGELIDTVVAQR"
BASE COUNT          532 a          435 c          534 g          556 t
ORIGIN      
        1 aacgccttct gtcagagtag cggttatgtc ttcttcgttg atacctttca gataaatttc
       61 cctgtggacg gaacccgaag aacgttcttg gcggatgatt tggccttttt catctttgtc
      121 gactttttcc gatttgcgtg tggctgaaat ggagagaatg tcgttttcgt aatccacagt
      181 gatatcctct ttggcatagc ctggcagatc ggcggtcact tggtagttat cctcggtttc
      241 gttgacatcc atttttattt cctggaagaa acgattttgg aacagcgatc cggtgtccga
      301 cagcaagtcg aagaaactgt tgcttctgcg taactcattt gacatttgaa aaatcccctt
      361 tcgttttttt gaacatttct ttcatcatca gtataaacct tcagacatca gaactccaca
      421 attttgaatg cggttccatt ttatgcacct gaagcaaaag ctgattccaa aaggtttcta
      481 tggtaaactg ttagaggagt tttgagacag taagcgcctg acgccgtatg aacgcgcggg
      541 ccgagaaagg gtagtatatc atgaaaaaaa ttgaagcggg cagtctctat ctgaaaaagc
      601 atcggaccct tttccagtgt ccaatctgca aagcggactt tgaaaaggtg gatgggcata
      661 gtatcatttg cgcgaatgga catcactttg atgtttccaa aaaggggacc ctgcatttgc
      721 tgatgaaggg cggccagaac gattacggca aggaaatgct gtcgagccgg aaaaatttgg
      781 cggacaccgg tttctttttc ccattgttgg atgccgtata tgcccatatc gacaagccgg
      841 agtcggcggc ggttctggat gtcggttgcg gggaaggatc gcatctgcat tatttgaccg
      901 aaaaaggctt gaaggggaca aaaataggct ttgatatttc gaaggatgcg atccagttgg
      961 cggccagcaa ctttttcccg gatgctttct ggtgtgtggc cgatctggcg cattcgcnnn
     1021 nnnnnnnacc aggaatttga ccgtgttttg aaaccaggcg gcaaggtcat caaagttgtg
     1081 ccgaatgcac agtatctgat cgaattgcgg gagcagttgt atgccttgga tacgacaaaa
     1141 agagagtacg acaatcaaca agtggtggat cgattctacg aaatctatcc ggatgcaatc
     1201 agcgaagaag tagcttatag tgtggctctt gatgcgaaaa gctatggctg gctgatggag
     1261 atgacgcctt tgtcatgggg tgcttctgaa acggcgttgg caaatgcggc ggcaaatcct
     1321 ttgaagtccg tcacagtggc agtcacactt ttggtcgcca gcaagtcagt ctgatttatg
     1381 tacaaccgga ctatgacctc aattttcaga aaaatgttga cacagcggtg tgatatgcta
     1441 taatacgtat atcattttgt agccattatt atcgttttgt ttcacaaaat gaccttgacc
     1501 aacgtatcgc attcgccgtg cctgacaccg aaacgatacg ttttttttgt gcgtattttc
     1561 gcaatctttt tcggaggtcg acaaggacac cgaacggtac acaaaatact gattgtcagg
     1621 agtataggaa atggattttg cagcattaaa ggctgaaata atggaagaaa gcaaaaaact
     1681 cggcatcgat aaaattggtt tcacgaccgc cgaacctttt gaatatatgt tggaaagtct
     1741 gaaggagcaa catgcgaagg gccatacggt cggctttgag cacccggtcc tggacgaacg
     1801 gatttatccg gaccggatat tcgataaccc gcagtcgatt ctttcgattg cgctggctta
     1861 tccgagcaaa atgaccgaaa aggctgagcg ggtgcgcgga gaacgccgcg gcaatttctc
     1921 gcgggcttcc tggggaactg actaccatgt cattctccgc gaaaggatgg accgcttgat
     1981 tgccttcatc caggaaaaga tgccggttgc ccgcttcaag ccgatggtgg acacggggga
     2041 gctgattgat acagtggtgg cgcaacg
//
LOCUS       DAOMUJ010000328         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15347_length_2063_cov_1.437251, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000328 DAOMUJ010000000
VERSION     DAOMUJ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15347_length_2063_cov_1.437251"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLT73_07715"
     CDS             complement(<1..564)
                     /locus_tag="PLT73_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HRM20300.1"
                     /translation="MHPFSIDCVSMMQKAVITISIVSLVKNSFIEPKQLLAAKTLKKS
                     SAFFLFCLLSLSVSIPLFLDGYATLEKFSADAGVIADKIPSFTIAGSALLLDEPKDKG
                     FILKTDTVLLALDNQEVYTQRELEKEMSSPDLILSLVFSKDAFILYAQDVPFRLPYEQ
                     ADGITDESIKKLLRNFSSTQFFSGVIML"
     gene            676..>891
                     /locus_tag="PLT73_07720"
     CDS             676..>891
                     /locus_tag="PLT73_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRM20301.1"
                     /translation="MAFTLPALPYAYDALTPYIDEETMHLHHEKHHNAYITNANAALE
                     KHPELADKTIEELLADLNSIPEDIRTAV"
     assembly_gap    892..901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <902..1282
                     /locus_tag="PLT73_07725"
     CDS             <902..1282
                     /locus_tag="PLT73_07725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRM20302.1"
                     /translation="GGHANHSLFWTVLAPNAGGEPTGAVKDGIEEAFGSFDAMKEKFS
                     AAAAGRFGSGWAWLVVSDGKLEITSTPNQDSPISEGKTPILGLDVWEHAYYLDYQNRR
                     ADYIKAWWNVVNWDEVNRRLAAAK"
     assembly_gap    1430..1439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1477..>1756
                     /locus_tag="PLT73_07730"
     CDS             1477..>1756
                     /locus_tag="PLT73_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM20303.1"
                     /translation="MEESIFERPLEDQLCFEVYRAANDFSRMYNRALKEFRLTFPQYL
                     VLLVLWDDNRILIKHIGERLGMGIGTLNPILNRLEKQGWLTKEPSLLDK"
     assembly_gap    1757..1766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1767..1954
                     /locus_tag="PLT73_07735"
     CDS             <1767..1954
                     /locus_tag="PLT73_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20304.1"
                     /translation="NAPRLKSASFSEKAVTSKKAIHVAILSEVKACNLEGIDGPFLMR
                     QLKLLNNAMSQADTGSV"
BASE COUNT          629 a          387 c          476 g          541 t
ORIGIN      
        1 aagcatgata acgccggaaa agaactgagt gctcgaaaaa ttgcgcaaca attttttgat
       61 ggactcgtcc gtaatgccat cggcctgctc atagggtaac ctgaatggga cgtcttgagc
      121 gtaaaggatg aaggcatcct tagaaaagac tagactcaaa atcagatcag gcgatgacat
      181 ttctttttcg agttctctct gtgtgtatac ttcctgatta tctaaagcca acaggacggt
      241 gtccgtttta aggataaaac ctttatcctt aggttcatcc aacagcaggg cgctaccagc
      301 aatagtgaat gaaggtattt tatccgctat cacacccgcg tccgccgaaa atttttccag
      361 ggtggcatac ccatccaaaa acaaggggat gctgacggaa agtgagagca agcagaagag
      421 gaaaaaggcc gaactctttt ttaaagtttt ggcagccagt aattgttttg gctcaataaa
      481 actatttttg actaaactga cgattgagat tgtgatcacc gctttctgca tcatacttac
      541 acagtctatc gaaaatggat gcatttagca atcacaaatt gttacggctt cgaggcatta
      601 tttttgataa agtgagcaat tgatgataca gtgaagtcag tcagaaatca caaacaattt
      661 taggaggaat ttaacatggc ttttacatta ccagcactac cttacgcgta cgacgctttg
      721 acaccatata tcgatgagga aacgatgcac ttgcatcatg agaaacacca caatgcatac
      781 atcacaaatg cgaatgcagc attggagaaa catcctgagt tagccgacaa aacaatcgaa
      841 gaattattgg ctgatttgaa cagcatcccg gaggatatcc gtacagcagt annnnnnnnn
      901 nggtggacat gcaaaccata gcctattctg gacagttttg gcaccgaatg cgggtggaga
      961 acctactgga gctgtaaagg atggaatcga agaagcattc ggcagttttg atgccatgaa
     1021 agagaaattc tccgcagccg ctgcaggccg tttcggatcc ggttgggctt ggctagtggt
     1081 ttccgatggt aaattggaaa tcacctccac tccaaaccaa gattcaccga tcagcgaagg
     1141 caaaacgccg attttaggtc ttgatgtatg ggagcatgcg tattacttgg attatcaaaa
     1201 tcgacgcgca gactatataa aggcatggtg gaacgttgta aactgggatg aagtcaaccg
     1261 tcgtttagcg gctgcaaaat aaccgaaaaa agatattgta atccaatcaa aggattcagg
     1321 gcacccgctc tgagtccttt tttcttacag tcataatata gtgatgactg aagaactgat
     1381 tttgtggatt tttcgagtgc gattgtttta ctttccggat gtcatccatn nnnnnnnnna
     1441 gttgtgaaaa taaatttgga aaggagacgc tgtcggatgg aggaatctat ttttgaaaga
     1501 ccgcttgaag atcagctttg tttcgaggtt tatcgagcag cgaatgattt cagtagaatg
     1561 tataaccgcg ctttgaaaga gttccgtttg acattccctc aatatcttgt gctgttggtt
     1621 ttgtgggacg ataatcggat attgatcaag cacatcgggg agcggttagg gatggggatc
     1681 ggaacattga accctatatt gaaccgactt gagaaacagg gttggctcac aaaggaacca
     1741 tcgcttctgg ataaacnnnn nnnnnngcaa cgcaccgcga ctgaaatcag cgagttttag
     1801 tgaaaaagcc gtcacttcaa aaaaagcaat tcatgttgct atattgtcgg aagtgaaagc
     1861 ttgtaacctg gaaggtattg atggcccgtt tttgatgagg cagttgaagc tgctcaacaa
     1921 cgctatgagc caggccgata ccgggagtgt ctaaaccaga acagtaatta aatgtgaata
     1981 atcgagtcat ttttcattca ttatataaaa cgataaataa aaaaagggag atgaattaat
     2041 gtttttagct tggaaagaaa tta
//
LOCUS       DAOMUJ010000329         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15472_length_2051_cov_2.038076, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000329 DAOMUJ010000000
VERSION     DAOMUJ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15472_length_2051_cov_2.038076"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PLT73_07740"
     CDS             <1..116
                     /locus_tag="PLT73_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRM20305.1"
                     /translation="PGIYLAGLGGVRIEDDLVIRANGNEILTHSPKELIIL"
     gene            185..742
                     /gene="efp"
                     /locus_tag="PLT73_07745"
     CDS             185..742
                     /gene="efp"
                     /locus_tag="PLT73_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004047589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRM20306.1"
                     /translation="MISVNDFKTGLTIEIDGGLWKVVDFQHVKPGKGAAFVRSKLKNL
                     RTGAVQEKTFRAGEKVGKAHIANRKMQFLYESAGAYVFMDTENYEQIELNAGQIEYEL
                     NYLKENMEVNILMYESETIGVDLPNTVILRVEETEPGIKGDTASGGSKPAKMETGLIV
                     NVPFFVNADDMLIVNTTDGSYVSRA"
     gene            769..1194
                     /locus_tag="PLT73_07750"
     CDS             769..1194
                     /locus_tag="PLT73_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM20307.1"
                     /translation="MMEETNIPLANGPAPLGEIEIAPEVIEVIAGIAANEVEGVYAMQ
                     GSFKTGVNELLGRTTHNKGVHLTVDENGLSVDVYCYIKYGASVPKVALDMQEKVKEQV
                     LYMSNLEVSQVNVHVVGLVAPKSEDSTYLDLDTELGENA"
     gene            1191..1646
                     /gene="nusB"
                     /locus_tag="PLT73_07755"
     CDS             1191..1646
                     /gene="nusB"
                     /locus_tag="PLT73_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRM20308.1"
                     /translation="MSNSRLTRREIREKALQALFQLKTNEELTPEEAIKQALLSDSEE
                     WNSEEDVEIQDYPYLITLVHGVLDNQAAIDEAIQPYLKNWTVGRLAKADALIMQIAAY
                     EMLLGDTEQVPQRVALNEAIEISKLYCDEQSSKFINGVLSNLIPKTENP"
     assembly_gap    1676..1685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1777..>2051
                     /locus_tag="PLT73_07760"
     CDS             1777..>2051
                     /locus_tag="PLT73_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRM20309.1"
                     /translation="METIIMDGKGLAKKMQDQMKKQVAALTAEGKTPGLCVILVGEDK
                     ASQVYVRNKEKQSEAMGMNSRVVRLAADISEADLLAEVEKQNQDDSM"
BASE COUNT          659 a          378 c          505 g          499 t
ORIGIN      
        1 aaccgggcat ctatctcgct ggtcttggcg gtgtccggat tgaagatgat cttgtgatcc
       61 gtgctaacgg caacgagatt ttgacgcatt ctccaaaaga gttgattatt ttataaaaaa
      121 atagaatccg cattaaaata atggtaaact tagttcaaat gagctgccac aggaggaatt
      181 ataaatgatt tcagtaaatg attttaagac aggcttgacg atcgaaattg atggagggtt
      241 atggaaagtt gtcgatttcc aacacgtaaa accaggtaaa ggtgcggctt ttgtccgttc
      301 aaaattgaag aatcttcgca ctggagctgt acaggaaaaa actttccgtg cgggtgaaaa
      361 agtcggaaaa gcgcacatcg cgaaccgtaa aatgcagttt ctttacgaga gtgccggtgc
      421 ttacgttttc atggacacag aaaactatga acaaatcgaa ttgaacgcag gccaaatcga
      481 gtacgaattg aactatctga aagaaaacat ggaagtcaat atcctgatgt atgaatcaga
      541 aacaatcggt gtcgatcttc cgaatacagt aatcctgcgc gtggaagaaa ctgagccagg
      601 aatcaagggc gatacagctt caggcggttc aaaaccggct aaaatggaga ctggattgat
      661 agtcaatgta ccattcttcg tgaatgcgga tgatatgctt atcgtcaaca caacggacgg
      721 ctcatacgta tcccgcgcat agtgtttccc aagaaaggag aatgacagat gatggaagaa
      781 acaaacattc ctttagcaaa cggtccggct ccgctgggag agattgaaat tgctcctgaa
      841 gtgattgaag taattgctgg tattgcagcg aatgaagtag aaggtgttta tgcgatgcaa
      901 gggtctttca agaccggcgt caatgaactg ttgggtcgca ctacccataa caaaggcgta
      961 catttgactg tcgatgagaa tggtttgtcg gttgatgtct attgttacat caaatacggc
     1021 gcttccgtgc cgaaagtcgc tttggatatg caagaaaaag tgaaagaaca agtcctttac
     1081 atgagcaatc ttgaagtgtc gcaagtgaac gtccatgtgg tcggtctcgt tgcaccaaaa
     1141 tccgaagata gtacttatct tgatttggat acagaattgg gggagaatgc gtgagcaatt
     1201 cccgtttaac aagaagagaa atccgtgaaa aggcgcttca agctcttttt caattgaaga
     1261 caaacgaaga attgacccct gaagaagcca tcaagcaagc tttgttgagc gattcagaag
     1321 aatggaacag cgaagaggat gtcgaaattc aagattatcc ttacttgatc acattggtgc
     1381 atggcgtatt ggataatcaa gcggcgatcg atgaagcgat tcaaccttat ctgaagaact
     1441 ggaccgtggg cagactggcc aaagcagacg ctttgatcat gcagattgct gcttatgaga
     1501 tgctgttggg agatacggaa caggtacctc aaagggtagc gttgaatgaa gcaatcgaaa
     1561 tttcaaagct atattgcgat gaacaatcga gcaaattcat caatggcgta ttgtccaacc
     1621 tcattcctaa aacggaaaat ccttaatgct tcggcataag ggttttcttt ttttgnnnnn
     1681 nnnnngaaat cggatatacc aggcccgctt ttctaactgc aatcaaagat attatggtaa
     1741 aataaacgat aacaccgagg aaaaggagat tgattaatgg aaacgattat catggatgga
     1801 aaaggcttag ccaaaaaaat gcaggaccag atgaaaaaac aagtagcagc attgacagcg
     1861 gaaggaaaaa ctccggggct ttgcgtcatt ttggtcggcg aggataaggc aagtcaagtc
     1921 tatgtccgca ataaagagaa acaatccgaa gcaatgggca tgaattcgcg agtcgttcgc
     1981 cttgcggcag acatttccga agcggatctg ctggctgaag ttgaaaagca gaaccaagac
     2041 gacagtatgc a
//
LOCUS       DAOMUJ010000330         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15501_length_2048_cov_1.584546, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000330 DAOMUJ010000000
VERSION     DAOMUJ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15501_length_2048_cov_1.584546"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLT73_07765"
     CDS             complement(<1..374)
                     /locus_tag="PLT73_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM20310.1"
                     /translation="MIHFVGAGPGDPELMTAKGVRLLQHADAVIYDRLVNPLLLFHCK
                     QDCRFIYVGKTPDKPSIRQAEINGKLIETAESYESVVRLKGGDPVIFGRLTEELEVCQ
                     NAGMRFDITPGITAASGTAGYSG"
     gene            complement(371..688)
                     /locus_tag="PLT73_07770"
     CDS             complement(371..688)
                     /locus_tag="PLT73_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H) small subunit"
                     /protein_id="HRM20311.1"
                     /translation="MTEKIFLTTTDALFPQIGRSVSIGNKKIAIFQLSDGRITAIEDF
                     CPLTGGPVLEGTVSGEYLYEPLRDYKISLLDGRIQDPDEGQLKVYELLLEDGDIYLKD
                     ASL"
     gene            complement(788..>2048)
                     /locus_tag="PLT73_07775"
     CDS             complement(788..>2048)
                     /locus_tag="PLT73_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRM20312.1"
                     /translation="IKDNRIEGIVLYGETADGNRYYNMMKKGQEIADLQSIAILQAAG
                     EEAGSDDVVAWDDDETVCGCNGVTKGDIIRGIRDKGLTNVAEVGKVTKAGTSCGKCKP
                     QVQTLLEHELGEGVTASTGICSCTDLNRDQVMTQIYAKHLTTSTDVFKELHFANPEGC
                     AKCRPAVNFYLNVAFPTEHKDERQSRYVNERMHANIQKDGTFSVIPRMRGGQTNPQQL
                     LQIAKVAEKYSVPLVKVTGSQRIGLYGVKKQELPAIWKELDMVSAQAYAKAFRSVKTC
                     VGKNFCRFGTQDSMGLGIKLEERFEFIDTPHKFKVGVSACPRSCVESGVKDFGIICVE
                     NGYQIYIGGNGGTEVKEAQLLTTVETEEEVIEYCGALLQYYRETGIYGERTAPWMERL
                     GFEAVKHILGDAAKRKDLIEAWMWQLQ"
BASE COUNT          439 a          566 c          471 g          572 t
ORIGIN      
        1 ccgctgtagc cggcagtgcc gctggcggca gtgatcccgg gtgtaatgtc gaagcgcata
       61 ccggcgttct gacaaacctc taattcttcc gtcagacggc cgaaaatgac aggatctccg
      121 cctttcagtc ggacgactga ctcgtaagat tcggctgttt caatcagttt tccattgatt
      181 tctgcctgtc tgatcgaggg cttatcaggt gttttgccga catagatgaa acggcaatcc
      241 tgcttgcaat gaaagagcag caacggattg accagccgat cgtagatgac cgcatcggca
      301 tgctgcaaca gccgcacacc ttttgcagtc atcaattccg gatctcccgg tcctgcccct
      361 acgaaatgga tcatagcgaa gcatccttca agtaaatgtc cccgtcttca agcaacagct
      421 catatacctt caattggcct tcatcggggt cctggatgcg cccgtcaagc aaggatattt
      481 tataatcccg cagcggttca taaaggtatt cgcctgatac ggtcccttcc agaacaggtc
      541 cgccagtcaa tgggcaaaaa tcttcgatgg ccgtgatacg gccgtctgat agttggaaaa
      601 tggcaatctt tttgttcccg atcgatacgg atcgtccgat ctgcggaaac aaagcatctg
      661 ttgtggtcaa aaatattttt tctgtcattt taatcaccca ccgttacaag ttctcttctg
      721 tctattgaat ataatttttc ctggatatcg cgatcgccga cgacttcgtg ccatggatct
      781 ttccgtttca ctgcagttgc cacatccaag cctcgatcaa gtctttgcgc ttggcggcat
      841 cgcccagtat atgcttgacg gcctcaaacc ctaaccgttc catccatgga gcggttctct
      901 cgccgtaaat gccggtttca cgatagtact gcagcaaagc accgcaatat tcaatgactt
      961 cttcttccgt ttccactgtc gtaagcaact gcgcttcctt gacttcagta ccgccatttc
     1021 cgccgatata gatctgatag ccgttctcga cacaaatgat gccgaaatcc ttcacgccgg
     1081 attctacgca gctgcgcgga caagctgata cgccaacttt gaatttgtgc ggtgtgtcga
     1141 tgaactcaaa gcgttcttcc aacttgatgc caagccccat cgaatcctgg gtaccaaatc
     1201 tgcagaagtt cttcccgacg caagttttga ccgagcggaa agctttcgca taagcctgtg
     1261 ctgaaaccat gtccaattct ttccagatgg ccggaagctc ttgcttcttg acgccgtaca
     1321 ggccgattcg ttggcttcct gtgactttca ctaatggcac gctgtatttt tcggctactt
     1381 tggcgatctg cagcagttgc tgcggattcg tctggccgcc cctcattctc ggaatgaccg
     1441 agaacgttcc gtctttttgg atattggcat gcatgcgctc gttcacatag cgtgattggc
     1501 gctcatcttt atgttcagtc gggaaggcaa cattcaaata gaaattgact gccggtctgc
     1561 atttcgcgca gccttcagga tttgcaaaat gcagttcttt gaatacatcc gtgctggtcg
     1621 tcagatgctt cgcataaatc tgagtcatga cttggtccct gttcaaatcc gtgcaggaac
     1681 agatgccggt tgaagctgtg acgccctcac caagttcgtg ctccaacaaa gtctgtactt
     1741 gcggcttgca ttttccgcaa ctggttccgg ctttggtgac ttttccgact tcagctacat
     1801 ttgtcaagcc tttgtcacgg atgcctctga tgatgtcgcc tttcgtgacg ccgttgcagc
     1861 cgcagacggt ctcatcgtca tcccaagcaa cgacatcatc gctgcctgcc tcttcacctg
     1921 cggcctgcag aatcgcgatc gattgcagat cagcgatttc ctggcctttt ttcatcatgt
     1981 tgtagtaacg gtttccgtcc gctgtttcgc cgtagaggac gatcccctcg atgcggttgt
     2041 ccttgatg
//
LOCUS       DAOMUJ010000331         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15517_length_2046_cov_2.323456, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000331 DAOMUJ010000000
VERSION     DAOMUJ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_2046_cov_2.323456"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1485
                     /locus_tag="PLT73_07780"
     CDS             91..1485
                     /locus_tag="PLT73_07780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM20313.1"
                     /translation="MKYEFPEDFWWGSAASGPQTEGTVAGDGKGDNIWDHWYKQNPEL
                     FFNQVGPENTSYVYNRYKEDIALMKKTGHTTYRTSIQWSRLIPDGIGAVNPEAVAFYN
                     SYIDELLANGIEPFMNLYHFDMPLSLQQKGGWESKEVVDAYADYARICFELFGDRVKK
                     WFTHNEPIVPVEGGYLYQFHYPSVVDLKKAVQVAYHETLASAKAIKIYHEMNLDGQIG
                     IILNLTPSYPRNENDPEDVKAARLADAFFNRSFLDPAIKGTFPEDLVLLLKELDYLPE
                     YTQSELELIKHNTIDILGINYYQPRRIMKKESTERLSDKPMPDDYFDNYIWPERKMNP
                     YRGWEIYEKGIYDCLINVRDNYGNLPCFISENGMGVEGEERFVNADGMIEDDYRIEFV
                     QDHLKYVHQALQEGCNVKGYHMWTCMDNWSWTNAYKNRYGFIAVDLTQEGKRTIKKSG
                     YWFKEVSDNNGFDA"
     gene            1510..>2046
                     /locus_tag="PLT73_07785"
     CDS             1510..>2046
                     /locus_tag="PLT73_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20314.1"
                     /translation="MNNFIDNLAEKLTPLAGKLGSNRYLAVLRDAFMLSFPLTMFGSI
                     VVVLNNLPFWSDDLKGTLGGLFGNGQNATMSIMTIFVTFGIGYYLTRSYDEDGVFGGA
                     VSLASYLILTPFNFTTAEGAEVSGALSLDRLGAKGMFIGMLAAFIAAEIYVRITKKGI
                     VIKMPEGVPDAIARSFASL"
BASE COUNT          569 a          453 c          490 g          534 t
ORIGIN      
        1 attttcgaat gaaatcgatt gcattttctt ttatcgtttt gtataattct atttgaattt
       61 atgtatagac atagaaacgg agttgtttag atgaagtatg aatttcctga agatttctgg
      121 tggggttcgg cagcaagcgg accacaaaca gagggtacgg tggccggaga cggcaagggg
      181 gacaatatct gggatcattg gtacaagcag aaccctgagc tgttcttcaa tcaggtagga
      241 cccgaaaaca catcttatgt atacaatcgc tacaaagaag atatagcact catgaaaaaa
      301 acgggacaca cgacgtatcg aacctccatc caatggagtc gtctgattcc ggatgggatt
      361 ggcgcagtga atccggaagc agtggctttc tacaatagct acatcgacga gttgttggcc
      421 aacggcatcg aaccttttat gaatctttat cactttgata tgccgttgtc attacaacag
      481 aaaggaggct gggaaagcaa agaagtcgtg gatgcctatg cagattatgc gcgcatctgc
      541 ttcgagctgt tcggcgaccg cgtaaaaaaa tggttcaccc acaatgaacc gatcgttccg
      601 gtcgaaggtg gctatcttta ccaattccac taccctagtg tcgtggatct gaaaaaagcc
      661 gttcaagtcg cttatcatga aactttggca agcgcaaaag ccatcaaaat ttatcatgaa
      721 atgaaccttg acggacaaat cggcatcatc ctcaatttga cgcctagtta tccgcgcaat
      781 gagaatgatc ctgaagacgt taaggcagcc cgtcttgctg acgcattctt caaccgctcc
      841 ttcttggatc ctgccatcaa aggcaccttc cccgaggatt tggttttgct gttgaaggaa
      901 ttggattatc taccggaata tacgcaatcg gaactggaac tcatcaaaca taataccatc
      961 gacattctgg ggatcaatta ctatcagcct aggcgcatca tgaaaaaaga aagcacagaa
     1021 cgcttgtcgg ataaaccgat gccggatgat tacttcgata actatatctg gccggaacgc
     1081 aaaatgaatc cgtaccgcgg ttgggaaatc tacgaaaaag gtatttacga ttgcctgatc
     1141 aatgtccgtg acaactacgg caacctgcct tgcttcatct ccgaaaacgg gatgggtgta
     1201 gagggcgaag agcgcttcgt caacgccgat ggcatgatcg aagatgatta ccgtatcgaa
     1261 tttgtccaag atcatctgaa atatgtccat caggcgctgc aggaaggttg caatgtcaaa
     1321 ggctaccaca tgtggacgtg catggataac tggtcctgga ccaacgccta caaaaaccgc
     1381 tatggcttca tagcagtgga tctaacgcaa gaaggaaaac gcacaatcaa gaaaagtggc
     1441 tactggttca aagaggtttc tgacaataat ggttttgacg catagttttt ctaagagagg
     1501 aaatgactaa tgaataattt tattgataat ttagccgaaa aattgactcc gcttgccggc
     1561 aaactcggct caaacagata tttagcagta ttgcgtgacg cttttatgct ttctttcccg
     1621 ctgacgatgt tcggatcgat cgtagtggtg ctgaacaatt tgccattttg gagcgatgac
     1681 ttgaaaggga ctttgggcgg cctcttcgga aacggtcaaa atgcgaccat gtcgatcatg
     1741 acgattttcg tcacattcgg tatcggctat tacctgaccc gctcttatga tgaggacggc
     1801 gtctttggtg gagccgtatc attggcttcc tacctgattc tgacgccttt caacttcacg
     1861 actgcagaag gcgccgaagt aagcggtgcg ctgtccttgg atcgtttggg tgcaaaaggg
     1921 atgttcatcg gcatgctggc tgcctttatc gcagctgaaa tctatgtccg catcacgaaa
     1981 aaaggtatcg tcatcaagat gcctgaaggt gttcctgatg ccattgcccg ctccttcgct
     2041 tctctg
//
LOCUS       DAOMUJ010000332         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15558_length_2042_cov_2.012079, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000332 DAOMUJ010000000
VERSION     DAOMUJ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15558_length_2042_cov_2.012079"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLT73_07790"
     CDS             <1..575
                     /locus_tag="PLT73_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM20315.1"
                     /translation="SVMRAIRAIERSDIVMVVLNAEEGIQEQDKKVAGYAHEAGKGII
                     ILVNKWDTLEKDNHTMKEFEEKIRKEFQYLSYAPIMYVSAVTKQRLSTIPAKIKEISE
                     NQNRRISSSLLNDVLMDAVAHNPTPTDKGRRLKIYYMTQVAVKPPTFVVFVNDPELLH
                     FSYERFLENRIRDSFDFEGTPFHLIARQKK"
     gene            779..1054
                     /locus_tag="PLT73_07795"
     CDS             779..1054
                     /locus_tag="PLT73_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM20316.1"
                     /translation="MANKAELIERVAGKTNLTKKDVTASVDALFEVIQEALKDGEKVQ
                     VIGFGNFEVRDRAARKGRNPQTGEEIQIEASKIPAFKPGKALKDAVK"
     gene            1257..>2042
                     /locus_tag="PLT73_07800"
     CDS             1257..>2042
                     /locus_tag="PLT73_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM20317.1"
                     /translation="MNKEAQAMIEAIQLFKQSVEQSVLENNSADLAELAETMHALGFL
                     SEAKEAYGALNYLAPQIQEWSIALAEIAIDEDDYDQALDILLTIDKESDVYPQALLTM
                     ADAYQVQGLYEVSEKKIFEAMALLPDEPILDYALAQLYHSIGEYKKAIPIYEELAFGN
                     GEIASATQLHFFLGDCHNAIGEFETALQYLERIPADEHFPDSYFQLGFTYFQLKEYAR
                     AISIFNKLLETDNAYLSAYLYLANALEAELELEEALAVLEQAIR"
BASE COUNT          645 a          384 c          479 g          534 t
ORIGIN      
        1 acagcgtcat gcgtgcaatc cgtgccatcg agcgttcgga tatcgtcatg gtcgtactga
       61 atgcggaaga gggtatccag gaacaagaca aaaaagttgc cgggtatgca catgaagcag
      121 gcaaaggaat catcatactg gttaataaat gggatacttt agaaaaagat aatcatacaa
      181 tgaaggagtt cgaggaaaaa atccgcaagg aattccagta cctctcttat gcaccgatca
      241 tgtatgtttc cgctgtgacg aaacaaagat tatctactat ccctgctaaa atcaaggaaa
      301 tcagcgaaaa tcagaatcgc cgtatttctt catccttgtt gaatgatgtc ctgatggatg
      361 ctgtcgcaca caatccaacg ccgactgaca aaggtcgtcg tctgaaaata tactatatga
      421 cgcaggtagc tgtaaaaccg ccgacatttg ttgtttttgt caacgatcca gaattactgc
      481 acttctcata tgagcgtttc cttgagaaca gaatccgtga ttcgttcgat tttgaaggaa
      541 caccatttca cttgatagct agacaaaaaa aataaattac cttcacaaag gcattaaatc
      601 cactataatc gttgcagtag cacggtttgt gtgctattct ttattagaaa tatcttttca
      661 atttgactgc ggtatttcag aactattttt ggaccactca aaaatagttt tcccatgttt
      721 tgttgtgtta tcaaagaaag catgtattct tcttctttaa aggaggtgaa aggacaacat
      781 ggcaaacaaa gctgagttaa tcgaaagagt tgctggaaaa acaaacttga ctaaaaagga
      841 tgttacagca agcgttgatg cattatttga agtgattcaa gaagcattaa aagatggtga
      901 aaaagttcaa gttatcggtt ttggtaattt tgaagttcgt gatcgtgcag ctcgtaaagg
      961 acgtaatcct caaactgggg aagaaatcca aatcgaagca agcaaaattc ctgcattcaa
     1021 accaggtaaa gcattgaaag atgcagttaa ataattcgtg aaaatgaagt tggggcctta
     1081 tggggcctca gctttatttt ttttggcgga aaatgatgat agtaatctgg gcagggtgtt
     1141 cgctgtgttg gacagagaaa ggtccgagga acaaatagat acagatgtcg aactttatga
     1201 taaaatgtaa aagaagagca ctagcaagaa gagcgtattg aaggagcagt tgacgaatga
     1261 ataaagaagc acaggcaatg atagaagcca ttcaactgtt caaacaatca gttgaacaat
     1321 ccgtcctgga gaacaacagc gctgatttag ccgaattggc agaaacgatg cacgccttag
     1381 gcttcttgag tgaagcaaaa gaggcatatg gcgcattgaa ttacctagca ccgcaaatac
     1441 aagagtggag tatcgcttta gcagagatcg ccatcgatga ggatgactac gaccaagctt
     1501 tggatattct gctcacgatc gataaagaaa gtgatgtgta cccgcaggct ttgttgacga
     1561 tggctgatgc ttaccaagta cagggcttgt atgaagtcag cgaaaagaaa atatttgaag
     1621 cgatggcgtt gttgccggat gaaccgatat tggattatgc actggcacag ctttaccact
     1681 caatcgggga gtacaagaag gcgattccga tttatgagga gcttgcattc gggaacgggg
     1741 aaatcgcatc cgcaacgcag ttgcattttt tcctgggcga ttgtcacaat gctatcggcg
     1801 aattcgaaac ggcgctccag tacctggagc gcataccggc tgatgaacat tttccggaca
     1861 gctatttcca actcggcttc acttatttcc aactcaagga atacgcgcgt gccatcagca
     1921 tttttaataa attgctcgaa acggataatg cttacctgag tgcgtatttg tacttggcaa
     1981 atgcgcttga ggccgaattg gagttggaag aagcgcttgc tgtgttggaa caagccatca
     2041 gg
//
LOCUS       DAOMUJ010000333         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15588_length_2039_cov_1.926411, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000333 DAOMUJ010000000
VERSION     DAOMUJ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15588_length_2039_cov_1.926411"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PLT73_07805"
     CDS             complement(<1..955)
                     /locus_tag="PLT73_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM20318.1"
                     /translation="MAQNNENKYNDDSIQVLEGLEAVRKRPGMYIGSTDARGLHHLVY
                     EIVDNAVDEALSGFADKIDITIHEDQSISVKDNGRGMPTGLHASGVPTIQVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSEWLEVSVIRESTVYTMKFKDGGKPASKLTKA
                     KSAAKGSGSSIHFLPDKEVFGTAQLSYEVLAERFRESAFLLKGLTITLKDERTEQSDT
                     FYFEEGIKEFVAYLNEEKDTLHDVVYFSGEADGIEIEFSFQYNDGYSETILSFVNNVR
                     TKDGGTHETGAKTAITKAFNEYARKMNLIKEKEKNLEGSDVR"
     gene            1138..1740
                     /gene="plsY"
                     /locus_tag="PLT73_07810"
     CDS             1138..1740
                     /gene="plsY"
                     /locus_tag="PLT73_07810"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004047847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRM20319.1"
                     /translation="MTAVVIVLAYLLGSIPSGIWIGKYFYHTDIRTYGSGNSGTTNTF
                     RVLGKKAGTIVLIMDMLKGSAATLLPIWLGAEIHPLLAGVVAALGHTYPLFAGFKGGK
                     AVATSGGIILAYNPLFFTEAIIAFVIYLYFSRMVSLSSILVCFTAIFLSFFFKDWPLT
                     IVTILLTVFIIYRHRSNIYRIKNGTESKVPFGYKSKSVKK"
     gene            complement(1811..>2039)
                     /locus_tag="PLT73_07815"
     CDS             complement(1811..>2039)
                     /locus_tag="PLT73_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRM20320.1"
                     /translation="LASKKGEHGVTMSYEDFDYLGIWTKPQQDATYVCLEPWCGLADR
                     SDSDGVLERKTAIQSLEPGNKRYYVHRIAFF"
BASE COUNT          521 a          484 c          419 g          615 t
ORIGIN      
        1 cgcggacatc actgccttcg agattcttct ccttctcttt gatcaggttc attttccggg
       61 cgtattcgtt gaaggctttg gtgatggctg tcttcgcacc ggtttcgtgg gtgccgccat
      121 ccttagtgcg gacgttgttg acgaaagaca aaatcgtctc cgaataacca tcgttatact
      181 ggaaggaaaa ttcaatttca atcccatctg cttctccact gaaatacacg acatcatgca
      241 gtgtatcttt ttcttcattt aagtaagcaa cgaattcctt gattccttct tcgaagtaaa
      301 aagtatcgct ttgttccgtc cgttcatctt tcagcgtgat ggtcaaccct ttcaataaaa
      361 aggctgattc cctgaaccgc tccgccaaaa cctcatagga taactgcgct gttccgaaaa
      421 cttccttgtc gggcaaaaag tgaatcgaag agcctgaacc ttttgcggca ctttttgcct
      481 tagtcaattt actggcaggt ttcccgccat ccttgaattt catcgtatag actgtgctct
      541 ctcggatgac ggacacttcc aaccactcgg acagggcatt gacgacactt gcacccacac
      601 catggaggcc tcctgaagtt ttgtagcctc cttgaccgaa tttgccacca gcatgcaaca
      661 ctgtgaaaat gacttggatg gttggtacac ctgaagcgtg taacccagtc ggcatgccgc
      721 ggccattatc tttgacacta atgctttggt cttcgtggat cgtgatatcg attttatcgg
      781 caaaccccga taaagcctcg tcaactgcat tgtcgactat ttcatatact aaatgatgca
      841 gtccacgtgc gtcggtcgaa ccgatataca tacctggtcg tttcctgact gcttcaagtc
      901 cttccaacac ttggatggag tcgtcattgt atttattctc attattttgt gccataaaaa
      961 aactccttca tctctgttct gaaccgaaca tctattctat catactgtat aattttcact
     1021 ttgaaaagta ttaaaaataa gacagaattc tgactcctat aggaaaaatg tggattaaat
     1081 ggtaaaatga actggaattg ttttatattg gagttttaaa ttaggagtga agtgagaatg
     1141 acagcagtcg tcatcgtcct ggcctatctt ttgggttcca tcccttcagg catttggatt
     1201 ggaaaatatt tttatcatac agacatccgc acatacggca gcgggaacag cggaacgacg
     1261 aataccttcc gcgttttagg gaaaaaagct ggtacaatcg tcttgatcat ggacatgctg
     1321 aaaggctcgg cggcaacttt actaccgata tggttagggg ctgaaattca ccctttgctg
     1381 gccggtgttg tggcagcatt agggcatacc tatccccttt ttgcgggatt caaaggtggt
     1441 aaggcagtcg ctacaagcgg cggcatcatt ttggcttata accccctttt tttcacagaa
     1501 gccatcatcg ctttcgtcat ctatctttat ttttccagaa tggtcagctt atccagcata
     1561 ttggtctgtt ttacggcaat tttcctttcc ttctttttta aggattggcc gttgacgatt
     1621 gtgacgatcc ttttgacggt tttcatcatc tatcgtcacc gttccaacat ttaccggatc
     1681 aagaatggga cagaaagcaa agtacctttc ggctacaaat caaagagcgt caaaaaataa
     1741 aagaataagg caggaccgca aatcagcaat gatttacggt cctgcctttt tagtgtaact
     1801 aggcaacgat ctagaagaac gcgatcctgt ggacataata acgtttattt cctggttcaa
     1861 gtgattggat ggctgttttc cgttccaaca ccccatccga atccgacctg tcagccaatc
     1921 cgcaccaagg ttccaaacag acgtaagttg cgtcttgctg cggtttcgtc caaataccta
     1981 agtagtcaaa atcttcatag gacattgtaa ctccgtgctc gcctttttta gatgccaac
//
LOCUS       DAOMUJ010000334         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15592_length_2039_cov_1.663306, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000334 DAOMUJ010000000
VERSION     DAOMUJ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15592_length_2039_cov_1.663306"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLT73_07820"
     CDS             complement(<1..319)
                     /locus_tag="PLT73_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HRM20321.1"
                     /translation="MMSNQAAKEIQAKKNMPKKWQGLKPARFAKYRSWINSGTPGICG
                     TYCSAVMVHDAVLQKYKKSLDKDTLLAGLLTVVDEFLPYKGTFFWDVQHGLKTLLKDI
                     PDWS"
     gene            complement(374..937)
                     /locus_tag="PLT73_07825"
     CDS             complement(374..937)
                     /locus_tag="PLT73_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM20322.1"
                     /translation="MIYLQLFWSFFQIGLFTIGGGYASLPLIRNEIVVNHGWLTMQEY
                     TDIITISQITPGPIAINSATFVGTKVGGFAGAIVATLGTVTPSIILSLILAWAYYKYR
                     DIKIMQGILGGLRPAVVALIAAAGLALFVAALFQSGGTLIGGITVNFLAVGIFIAALY
                     ALRTTKIDSIWLILVSGAVSILFHLIS"
     gene            complement(934..1512)
                     /locus_tag="PLT73_07830"
     CDS             complement(934..1512)
                     /locus_tag="PLT73_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM20323.1"
                     /translation="MANQEKSVRLFWLLFKSTFVLSAFTFGGGYVIVPLMQKKFVEQL
                     KWIDSDEMLDLVALGQSAPGAIAVNTSILVGYRVAGFLGALVTVFGTVAPPLIIITIV
                     SYFYMNFRENPIVDALMIGMQAGVAAVIVNVVIGMVSGVVKQRDIVNILLLLVAFIAS
                     YFFDVHVVLIIFLAGLIGFVNLSYQEKKGAIK"
     assembly_gap    1688..1697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1743..1838
                     /locus_tag="PLT73_07835"
                     /pseudo
     CDS             1743..1838
                     /locus_tag="PLT73_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003648690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(1819..>2039)
                     /locus_tag="PLT73_07840"
     CDS             complement(1819..>2039)
                     /locus_tag="PLT73_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20324.1"
                     /translation="EEADVLEFVTFLDFRNGGIGKSEFHDLIPFFPYKVTANDQAGKL
                     PIQLCFLTISIRYIHQKTRKAIFRRKSG"
BASE COUNT          605 a          494 c          419 g          511 t
ORIGIN      
        1 cggaccaatc cggaatgtcc ttaagcagag tcttcaatcc gtgctgcacg tcccagaaga
       61 aagtcccctt gtagggcaga aattcatcaa cgaccgtcag caagcccgcc aacagtgtat
      121 ctttatccaa agacttcttg tatttctgca gaaccgcatc atgcaccatc acagcgctgc
      181 aataagtgcc acagatgcct ggggtgccgc tgttgatcca gctgcggtat ttcgcgaaac
      241 gcgcaggctt caacccctgc cactttttcg gcatattctt ttttgcttga atctctttag
      301 ctgcctgatt tgacatcatg acaagttcct ttcttgacga agtgaatgtg ctattctttt
      361 attatacacc caatcagctg attaaatgga aaaggatgct tacggcacca gaaacaagta
      421 tcagccaaat agaatcgatt tttgtagtcc ggagcgcata caatgccgca ataaagattc
      481 caaccgcaag gaaattgacg gtgattccgc cgattagcgt tccgccggat tggaacagag
      541 cagccacaaa aagcgccaat cctgccgcgg caatcagagc tacgactgcc ggtcgcagcc
      601 caccaaggat gccttgcatg attttgatat cgcggtactt atagtaagcc catgccaaaa
      661 tcaatgagag gatgattgaa ggcgtgactg taccaagagt cgcaacaatt gcccctgcaa
      721 aaccgcccac tttggttccg acgaaggtag ccgaattgat ggcgatcggt cccggcgtga
      781 tctgcgaaat cgtgatgatg tccgtgtatt cctgcattgt tagccagcca tgattcacga
      841 cgatttcatt ccggatcagc ggcaaggacg cgtagccacc gccaatcgtg aacagaccga
      901 tttggaagaa ggaccaaaat aattgcaaat aaatcatttg atcgcccctt tcttctcttg
      961 atagctcaga ttgacaaaac cgatcaatcc cgccaagaaa atgatcagga cgacgtgaac
     1021 atcaaagaaa taggaagcga tgaacgccac caacagcaat agaatattca caatgtcgcg
     1081 ctgcttgacg accccgctga ccatgccgat gacgacattg acgatgactg cagccacacc
     1141 cgcttgcatc ccgatcatca aagcatccac aatcggattt tcacgaaaat tcatgtaaaa
     1201 ataggaaaca atcgtgatga tgataagcgg gggcgcaacc gtaccgaata cagtcaccaa
     1261 agctcccaga aaacccgcca cacgatagcc gaccagaatg gatgtattga cggcaatcgc
     1321 accaggcgcg gactgcccca aagcaaccaa atccaacatt tcatctgaat caatccattt
     1381 cagttgttcc acgaattttt tttgcatcaa cggtacgatc acatagcctc caccaaaagt
     1441 gaatgcactc agcacaaaag ttgatttgaa caatagccaa aacaaccgaa ctgacttttc
     1501 ttgatttgcc aaagaatctc cccacctctt cattatttta aactcatata tctttgattc
     1561 taccaaagtt ggttagtgat gtaaaaagaa cgatctacca gagggcagta gcaaaagatt
     1621 gcagaagtgt tattgacatt tttccagtta taggattttc ttaagaatgt tcaaaataaa
     1681 tcgaaaannn nnnnnnnatt tttgaggagt gaatcactat cgaagcttgg attcaaagca
     1741 ttatggagca atttggctac ttcggtgtag catttttgat tatgatcgaa aatctttttc
     1801 cgccgatccc ttctgaagtc atcctgactt tcggcggaag atggcttttc ttgttttttg
     1861 atggatatat cgtatactta tagtaagaaa gcaaagttgg ataggcagtt tccccgcctg
     1921 gtcatttgca gttactttgt atggaaagaa agggattaaa tcatgaaact cacttttacc
     1981 gattccgcca ttacggaaat ccaaaaacgt tacgaactca aggacatcag cctcttcta
//
LOCUS       DAOMUJ010000335         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15606_length_2037_cov_2.036327, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000335 DAOMUJ010000000
VERSION     DAOMUJ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15606_length_2037_cov_2.036327"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="PLT73_07845"
     CDS             <1..1791
                     /locus_tag="PLT73_07845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM20325.1"
                     /translation="KEDLTAALNRKNTVLFRMLSEGYITKEEYDAAIAYDITQDFVPA
                     NATQEDRNSYLYQAVEREAILVLMEQAAAANELTMDDLEADIELYNEYYANADNTLRL
                     SGYNVYSTIDKDVYNGMQEVAADYGQTLGETFVDSYVDANGVTQEVIELAQTGSVLIE
                     NQTGRIIGFIGGRDFSVNQVDHVFSTKRSPGSTIKPLLVYAPAIEQNLIYPASMVPDT
                     KISIKQPDGTLWEPTNFGDTISNTYVTARQALYQSKNNPTIKLYVAMNSKGMNPGQYL
                     EKMGITSIDESEYGNPSLAIGATNDGPTVLEQTSAFTTFATGGEHVSPYLIETITDKH
                     GNVVYQHESEATRVFSQDTAYLTLDILRDVITDGTGRSINNYLNFNADWAGKTGTSEA
                     NRDIWFIASTPTVTLSSWMGYDNYKGEHVFYDPNNQGLPTGRNLNYWSQIANKIYSIN
                     PGILGTDQTHQRPEGIVEKSVLAETGTLAGTVSFANGLSVTLNGGTRTDLFKADALPK
                     QISYYFSPGATDKDLEDYWDSYLAEAAARRNAAAAAAAAAAASQNNAANNADDAKKED
                     PTEEEDAETPPDTEPEGETPPADETPTTEE"
BASE COUNT          594 a          490 c          474 g          479 t
ORIGIN      
        1 aaagaagatt tgactgcagc ccttaaccga aaaaatactg tgcttttccg gatgctcagc
       61 gagggatata tcaccaagga agaatatgat gctgcaatcg cgtatgacat cactcaagat
      121 ttcgtacctg ctaatgccac acaagaggac cggaacagtt acctttatca agctgtcgaa
      181 cgtgaagcaa tccttgttct gatggaacag gccgcagccg caaacgagct tacgatggac
      241 gatctggaag ccgatattga attgtacaac gaatattacg ccaatgccga caacacattg
      301 cgcttgtccg gctacaacgt atacagtaca atcgacaaag atgtctacaa cggcatgcaa
      361 gaagtggcag cagattacgg acaaactttg ggcgaaacct ttgtggattc ttatgtcgat
      421 gcaaacggag tcacgcagga agtgattgag cttgcgcaaa caggcagtgt tcttatcgaa
      481 aatcagaccg gtagaatcat tggcttcatc ggcggacgag atttttcagt caaccaagtg
      541 gaccacgtat tcagcacgaa acgttctcct ggttccacga ttaaaccgtt gttggtctac
      601 gcaccggcga ttgaacaaaa tctgatttat cccgcctcga tggtgcctga tacaaagata
      661 agcatcaaac agccagatgg cacgttatgg gagcccacta acttcggcga caccatttcc
      721 aatacttacg tgaccgcgcg ccaggccctc taccaatcta agaataatcc gacaatcaaa
      781 ctctatgtcg ctatgaacag caaaggaatg aatcctgggc aatatttgga aaagatgggt
      841 atcacttcaa tagacgaatc ggaatacggc aatccgtccc tagcgattgg ggcaacgaat
      901 gacggaccga ctgttttgga gcagaccagc gcgtttacga ccttcgcaac tggaggcgag
      961 catgtatcgc cttacctgat cgaaacaatc acggacaagc acggaaatgt tgtttatcag
     1021 catgagtccg aagcgacaag agttttctcg caggatacag cttatttgac tttggatatt
     1081 ctgcgcgacg tcataactga cggcactggc cgttcgatca acaattatct gaatttcaac
     1141 gctgactggg ccggaaagac cggtacatcg gaagctaacc gtgacatttg gttcatcgcc
     1201 agcaccccta ccgttacgct cagctcttgg atgggttacg acaattacaa gggggaacat
     1261 gtcttctatg atccgaataa tcaaggactt ccaactggcc gaaatttgaa ttattggtcg
     1321 caaatcgcca acaaaatcta cagcatcaat ccgggcattc tcgggaccga ccagacgcat
     1381 cagcggccgg aaggcattgt cgaaaaatca gttttggccg aaaccggaac attggcagga
     1441 accgtgtcat tcgcgaacgg cttatccgtc acgcttaatg gtggaacaag gaccgactta
     1501 ttcaaagctg atgcgttgcc gaagcaaatt tcctactact tctcccccgg ggctaccgat
     1561 aaagacctgg aagactattg ggatagttat ttggctgaag cagcagcaag acgcaatgca
     1621 gccgctgcag cagcagcggc tgccgcagct agccaaaaca atgcggcaaa taatgccgac
     1681 gatgcaaaga aagaagatcc tacagaagaa gaagatgcgg aaacacctcc tgacacagaa
     1741 ccagaaggtg aaacaccacc cgctgatgaa actcccacaa ctgaagaata agcgaaaagg
     1801 ctgcccgtca tggcagcctt ttgtttttgg catttgtatt ttcgtcagca gtgccggaaa
     1861 acattccaaa tttcttgccc aaaaagccct gcatccattc aggatacagg gctttttggt
     1921 cttgagtgtc tcgcggatga acacattatt ttttaggtaa tgttgttcct ctgttgacaa
     1981 ttttgtatgg aaggatgatt gatttctcat caatttcttc cttgttcatt attttag
//
LOCUS       DAOMUJ010000336         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15615_length_2036_cov_2.147400, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000336 DAOMUJ010000000
VERSION     DAOMUJ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_2036_cov_2.147400"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLT73_07850"
     CDS             complement(<1..514)
                     /locus_tag="PLT73_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM20326.1"
                     /translation="MSTKKWLLKARLKEQHIEALKGLYPDHEMITNDAETEGFSVVEW
                     AVGWNDELTNLLEEGELPSLKWVQAISAGVNSLPLETFERKGIYLTNASGIHAEPIAE
                     YVIGVLLSHMRGLKAAILNQPQRKWEQTMELQELKGKTMMIVGAGHIGNRLGELAKAF
                     GMKVIAVNRSG"
     gene            complement(517..1346)
                     /locus_tag="PLT73_07855"
                     /pseudo
     CDS             complement(517..1346)
                     /locus_tag="PLT73_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
     gene            complement(1370..2011)
                     /locus_tag="PLT73_07860"
     CDS             complement(1370..2011)
                     /locus_tag="PLT73_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM20327.1"
                     /translation="MENKEVENWDSFLAPYEQAVEELKVKLKGIRKQYRDENTHAPIE
                     FITGRVKTKESILEKAEIRDIDLTRLEQDVQDIAGLRIMCQFVEDIHEVVRLLRARKD
                     FKIIIERDYITHKKESGYRSYHMVIEYPVQRIYEEKKILVEIQIRTLAMNFWATIEHS
                     LNYKYRGEYPEELHDRLIRAAEAAFRLDEEMSEIREEIKDAQRYFSDKKEDGR"
BASE COUNT          462 a          553 c          414 g          607 t
ORIGIN      
        1 taccgctgcg attgacagcg atgactttca taccgaatgc tttggccagt tctcccagcc
       61 gattcccgat gtgacccgca cctacgatca tcatcgtctt gcctttcagt tcctgaagtt
      121 ccatggtctg ttcccatttg cgttgcggct gattcaggat cgcagccttc aatccgcgca
      181 tatgcgaaag caatacgccg atgacgtatt cagcaatcgg ttcagcatgg atgccgctcg
      241 cgttcgtcaa gtagattccc tttctttcaa aggtttctaa gggaagactg ttcacgcctg
      301 ccgaaatcgc ttggacccat ttcaatgaag gcaactctcc ttcctccaac aggttggtca
      361 attcgtcgtt ccatccgaca gcccattcga ctacactgaa cccttctgtt tccgcgtcat
      421 ttgtaatcat ctcgtgatcc gggtagagcc ccttcagcgc ttcgatgtgc tgttccttca
      481 aacgcgcctt cagcagccac tttttggttg acatattcac cgctcctttg tccgttcgtt
      541 cctatcgttt ttcttcacgc cgatgaacgc atcctccacc cgatcccaga aatgcgtgtg
      601 acggtaacgc gcaaaatgca cgcgctcctg ggcaatccgg taaaccagtc gttcgatgct
      661 tcgtttcttg aatgtcaaat gatccacaga caaaatcaag cgatcttcct tgtgaggata
      721 aataacaatc cattcgtctt tcgctatgat catgggtgaa ctcagcgtcc gatagatgcg
      781 gttattcacc gaggccatct cggtcagctg gatagcttcc gtgcgaggat ggataacggc
      841 tccaccaagc gatttgctga acccggtcga acctgtagga gtggaaacac aaagaccatc
      901 accacggaat gtctcgaaca gctcatcctt cacgtagata tcgcaaacca tcgttccgtc
      961 cattttcttc aaacttgctt cgttcagagc gatgtagcgg gtttcttcct cctcatcagc
     1021 atacttgacg cgcacatcga gcagtggata gctgacgctt tcccctttgt catgcttcag
     1081 gctttccaca agttccggca gttcataagt acgccaatcc gtatagaagc ccagatgtcc
     1141 agtatggatt cccacaaatc gcacatggtt caattgttct tcgtatttat ggaaagccga
     1201 aagcaatgtg ccatcgcccc cgatcgatac cacaagatcc ggatggtccg tgataatttc
     1261 aatttttcag agaggcacat ctctttgaac ttcgccgcaa cggataacgt ctctggagtc
     1321 tggttatgga ccagtgcaat tttcatatcc gatctcctct cgaataattt tatcgtccat
     1381 cttctttctt atcggaaaaa taacgttgag catccttgat ttcttcgcgt atttccgaca
     1441 tttcttcgtc aagacgaaag gcagcttctg ccgcgcgtat cagacggtca tgcaattctt
     1501 cgggatattc gccccgatat ttgtagttca acgaatgttc gatcgttgcc caaaaattca
     1561 tggctaatgt cctgatctga atttcgacga gtattttttt ctcttcataa atgcgttgga
     1621 ccggatattc gatcaccata tggtacgagc gatagccgct ctctttttta tgcgtaatgt
     1681 aatcccgttc tatgatgatc ttaaagtctt ttcttgcgcg caaaaggcgc acaacttcgt
     1741 gtatatcctc tacaaattgg cacatgatcc gcaatccggc aatatcctgt acgtcttgtt
     1801 ccagtctggt caagtcgata tcccggattt ccgccttttc caatatgctt tcctttgttt
     1861 tgacacgtcc cgtaatgaat tcgatggggg cgtgcgtatt ctcatcgcga tattgcttgc
     1921 ggatcccctt cagcttaact tttagttcct ctaccgcttg ttcgtacggc gctaaaaatg
     1981 aatcccagtt ttcaacttcc ttattttcca tagaacgaca ccctatcata tagtat
//
LOCUS       DAOMUJ010000337         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15649_length_2033_cov_1.990394, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000337 DAOMUJ010000000
VERSION     DAOMUJ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15649_length_2033_cov_1.990394"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PLT73_07865"
     CDS             complement(<1..1539)
                     /locus_tag="PLT73_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRM20328.1"
                     /translation="MRKIDQIQIDVLAYFQEHAAKSMTLKQVSEALGYTESDEFKALV
                     KVFAQLEERGILMLNKTGQFRLKTQSATMTGTFRSNDRGFGFVTIDPNESDLFIPRGK
                     TGSAMEGDTVEVKITREAEPWNDRGVEAEVTAVLTRKSNQVVGEFVPYDSDRREETGF
                     LGFVIPQDTKINSIVVYIKPEGLHPVEGSVCLVEITSYPTPQNPIKMEGLVTKEIGHK
                     DAPGVDILAILYKFGIPSEFPEEVLAQAENIPLEIPAEALEGRRDLRSEQIVTIDGAD
                     AKDLDDAISLKRLENGNYLLGVHIADVSYYVTENSPIDREAYERGTSVYLTDRVVPML
                     PQKLSNGICSLLPHEDRLTLSCEMELDADGEVVAYDIFPSVIESKQRMTYSDVNAILM
                     DHDKKLRDKYSDFVSMFEDMAILHETLLKKRQKRGAIDFESDEAKIIVDENGHPLDIY
                     VQDRGVGERLIESFMLSANETIAHHFTKANLPFIYRIHEQPDPTKMMRFLEFVTTFGI
                     LVSGT"
     gene            complement(1542..>2033)
                     /locus_tag="PLT73_07870"
     CDS             complement(1542..>2033)
                     /locus_tag="PLT73_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HRM20329.1"
                     /translation="LAVFGLSMGGIMATRAIETGGFIGGGSFNSPIISFEDSRVPISF
                     MHFAKLSKKKLGMANLEIEHELALMKPKLNAQLAQLDEFTELIKANLADITVPHYIAQ
                     SGKDEMISADCGKLLADRLVNAEVDYHFFSEATHVITVGKDRNVFEETVLAFLNRLYW
                     NEG"
BASE COUNT          499 a          533 c          447 g          554 t
ORIGIN      
        1 cgtaccgctc acaagaatgc cgaaggtcgt cacaaattcc aggaagcgca tcattttggt
       61 cgggtccggt tgctcgtgga tccggtagat gaaaggcaga tttgccttcg tgaaatgatg
      121 ggcgatcgtt tcattcgcac tcaacatgaa agattcgatg aggcgctcgc cgacaccgcg
      181 atcctgcaca tagatgtcca gcgggtggcc gttttcgtcc acgatgattt tcgcttcatc
      241 ggattcgaaa tcgatggccc cgcgtttttg acgttttttc aaaagggttt catggagtat
      301 tgccatatct tcgaacatcg acacaaaatc gctgtattta tcacgtagtt ttttgtcgtg
      361 atccatcaag attgcgttga cgtcggagta ggtcatccgt tgtttcgact cgatgacact
      421 cgggaaaata tcataagcaa ccacttcccc atccgcatcc aattccatct cacaggataa
      481 tgtcaaacga tcttcatgcg gcagcaagga acagatacca ttcgataatt tttggggaag
      541 catcggaacg acccgatccg tcagatagac actggtgccg cgttcataag cttcgcgatc
      601 gattggagaa ttttccgtca cataatagga aacatccgcg atatgtacac ccaacagata
      661 attgccgttt tccagtcgtt tcagagaaat cgcatcatcc aaatccttcg catcggcgcc
      721 atcaatcgtg acgatctgtt ccgaacgcaa atcccgtctg ccttccaacg cctcggccgg
      781 aatctccaaa ggaatatttt cggcctgggc caacacctcc tccggaaatt cggaagggat
      841 gccgaactta tacaagatgg ccagaatgtc cacaccaggc gcatccttat ggccgatttc
      901 tttcgtgacc agcccctcca ttttgattgg attctgcggt gtcggatagc tggtgatttc
      961 aaccagacag acactgccct ccacaggatg aaggccttct ggcttgatgt agacaacaat
     1021 gctgttgatt ttagtatctt gtgggatgac aaaccccaaa aacccagttt cttcacgacg
     1081 gtccgaatcg taaggaacaa actcaccgac gacctgattc gacttgcgcg tcaaaacagc
     1141 agttacctcg gcttcgacac ctctgtcatt ccaaggctcg gcctcacggg tgattttaac
     1201 ttcgaccgtg tccccttcca tcgcgctgcc ggttttgccg cgcgggatga agaggtcact
     1261 ctcattcgga tcgatcgtca caaagccgaa accacgatcg ttgcttctga aagtgcctgt
     1321 cattgtggca ctttgcgttt taagacggaa ttgtccggtt ttgttcagca tcagaatccc
     1381 tctttcttcc aactgagcaa agacttttac tagtgctttg aattcatcgg attccgtgta
     1441 acccaacgct tcacttactt gcttcaaggt catgctcttt gcggcatgct cttgaaaata
     1501 ggctagaacg tcaatttgga tctgatctat ctttctcata cttatccctc attccaatat
     1561 agtcggttca aaaatgccaa aactgtttcc tcaaatacgt ttctgtcttt cccgacagta
     1621 ataacatgcg ttgcttcaga gaagaaatga taatccactt ctgcatttac gaggcgatcc
     1681 gccaacaatt tcccgcagtc ggcgctgatc atttcatcct tgccggattg tgcgatgtaa
     1741 tggggaactg tgatgtcggc aagattcgcc ttgatcagtt ccgtaaactc atccaattgg
     1801 gcaagctggg cattcaattt tggcttcatc agcgccaatt cgtgctcgat ttccagattc
     1861 gccataccga gtttcttttt cgacagcttg gcgaaatgca taaaactgat cggcacgcgg
     1921 gaatcctcaa aactgatgat tggtgaattg aaagatccgc caccgataaa tccgcctgtt
     1981 tcgatggctc ttgttgccat aatgcctccc atcgacagtc cgaaaacggc aag
//
LOCUS       DAOMUJ010000338         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15677_length_2030_cov_2.150886, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000338 DAOMUJ010000000
VERSION     DAOMUJ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15677_length_2030_cov_2.150886"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLT73_07875"
     CDS             complement(<1..1133)
                     /locus_tag="PLT73_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM20330.1"
                     /translation="MTELYYFDNSATTLKKPAAVAEAVYHAIADAQLGNPARGSHTAS
                     LNALRLTERLRQKTAALFGVPDAANVAFTANATASLNLVLKGLLKPTDHVITTVTEHN
                     AVLRPLYQLEEQGASLSFVGVDEFGTLLYSDFERLLRPDTRAVVVNHASNVTGNVADL
                     ERIGSFCREHDLLFIVDASQSAGVLDIDMVKLHIDVLCFTGHKGLYGPQGTGGICLGD
                     RPLEFVPVFTGGSGFHSFDKQYPTEMPTLFEAGTQNVHGLAGLSAGLDYISQKGLAAI
                     TSYLQELTFRFHDQIKNIPGITLYGSFAENMERAPVVSLNLEGWSSGDLSDALFMDYS
                     IAVRPGAHCAPLIHQAFGTEKNGMVRFSFSSFNTVEEIDYAAKA"
     gene            complement(1126..1398)
                     /locus_tag="PLT73_07880"
     CDS             complement(1126..1398)
                     /locus_tag="PLT73_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRM20331.1"
                     /translation="MIDRKHYGIVAFPTSQAAAAAEAIILDADRECRLIPMPEQISAG
                     CGLVLQTHLDELNAVRLLLSEQAISNDGCYEVHFENRIKTVEEWHD"
     gene            complement(1395..>2030)
                     /gene="yedF"
                     /locus_tag="PLT73_07885"
     CDS             complement(1395..>2030)
                     /gene="yedF"
                     /locus_tag="PLT73_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRM20332.1"
                     /translation="EETSMKKIDAVGQACPMPVILTKRALKESNGEAILISVDNEIAT
                     QNLAKMAEQLKLSVQVKKINDSLYEVQLSDPNAGNEAHAAEHTALPEQVTDTVAPSGA
                     SYVVVLNSDSIGNGSEELGRTLMKSFLFSLTEQDVLPDKVLCYNAGALLTTEGSAVLD
                     DLKALEEDGVEVLTCGICGEFYGVKDKLAVGTFTNMYRIVEILRTAKTVSP"
BASE COUNT          427 a          562 c          571 g          470 t
ORIGIN      
        1 gctttggctg cgtagtcgat ttcctccacc gtgttgaagc tagagaagct gaagcggacc
       61 ataccgtttt tttcagttcc aaaagcttga tggatgagcg gtgcgcaatg ggctccggga
      121 cgcacggcga tggagtagtc catgaagagc gcatcgctga ggtcgccgga ggaccagccc
      181 tccaaattca gcgaaacgac cggcgcgcgc tccatgtttt cagcgaagct accatagaga
      241 gtgattcccg ggatattctt gatctggtca tggaagcgga aagtcagttc ctgcaggtag
      301 ctagtgatcg ctgccagccc tttctggctg atgtaatcca aaccggcgga aaggcccgcc
      361 aagccatgga cattctgggt gccggcctcg aaaagggtcg gcatctctgt cgggtattgc
      421 ttatcgaaag aatggaagcc gctgccgccg gtaaacaccg gcacgaattc caagggcctg
      481 tcgccgaggc agatgccccc cgttccttgc gggccgtaga gtcctttgtg tccggtgaag
      541 cagagaacgt cgatgtgcag tttcaccatg tcaatatcga ggacgcctgc gctctgggaa
      601 gcatcgacga tgaacaacag atcgtgctcc cggcagaagc tgccgatccg ttccagatcc
      661 gctacgttgc cggtaacatt cgaggcatgg ttgaccacga ctgcccgggt atcgggtcgc
      721 aacaggcgtt cgaaatcaga ataaaggagc gtcccaaact catcaactcc gacaaaggat
      781 agagaagcgc cctgttcttc cagttggtaa agcggacgca ggacggcgtt gtgctccgtc
      841 actgtcgtga tgacatggtc tgttggcttc agcaagccct tcaggaccag gttcagcgat
      901 gcggtggcgt ttgccgtgaa agcgacgttt gccgcatccg gcacaccgaa cagggctgcc
      961 gttttctggc gcagcctttc cgtcaaccgc aaagcgttaa gagaagcggt atggctgccg
     1021 cgtgccgggt tgcccagctg agcgtcggcg atggcatgat agacagcttc agccaccgcg
     1081 gccggtttct tcagcgtggt tgcactgttg tcgaaataat aaagttcagt catgccattc
     1141 ctccacagtt tttatccggt tctcaaaatg cacttcgtag cagccatcgt tggaaatggc
     1201 ctgctccgac aacaacaagc ggacagcgtt cagttcgtcg agatgcgtct gcagcaccaa
     1261 tccgcatccg gccgaaatct gttccggcat gggaatcagc cggcattccc gatcggcatc
     1321 caggataata gcttcagcgg ctgccgcggc ctgagacgtc ggaaacgcga cgatgccgta
     1381 atgtttgcgg tcgatcatgg cgatacggtc ttggcggtgc gcaggatttc aacgatgcgg
     1441 tacatgttcg tgaaagtacc cacagccaat ttgtccttca cgccgtaaaa ttctccgcaa
     1501 atgccgcacg tcagcacctc gacgccgtct tcctccagcg ctttgaggtc gtccaatacg
     1561 gctgaacctt ccgtcgtcag caacgcgccg gcattgtagc agagcacttt gtcaggcaga
     1621 acgtcttgct ccgtcaggga aaagaggaag cttttcatca gcgttcgacc caattcctcg
     1681 ctgccgttgc cgatggagtc gctgttcaat acgacgacat aacttgctcc ggatggtgcg
     1741 actgtgtcgg tgacttgctc cggaagcgcg gtgtgttcgg cggcgtgtgc ttcgttaccg
     1801 gcattcggat ccgaaagctg gacttcataa agggaatcgt tgattttttt gacttggact
     1861 gaaagcttca gctgctcagc catcttggca aggttctgcg tcgcaatctc attgtccacg
     1921 ctgatgagta tggcctcccc gttgctctcc ttcaaagccc gtttggtcag aatgaccggc
     1981 attggacaag cttgtccgac tgcatctatt tttttcatgc tcgtttcctc
//
LOCUS       DAOMUJ010000339         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15782_length_2020_cov_2.096692, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000339 DAOMUJ010000000
VERSION     DAOMUJ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15782_length_2020_cov_2.096692"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..689)
                     /gene="coaE"
                     /locus_tag="PLT73_07890"
     CDS             complement(90..689)
                     /gene="coaE"
                     /locus_tag="PLT73_07890"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772790.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRM20333.1"
                     /translation="MSYILGLTGSIATGKSTVAKLFLSAGIPVVDADLGARAVVLPGA
                     PGLADIIEHFGEAYLLSDGTLDRKRLGALIFSDREKRKELDVLLKERINDWIQAEKER
                     YISEGHKLIVLDIPLLYVGGYEDSCDAVMVVYVPEELQVQRLMSRNHLDADEATRRMQ
                     SQLSIEKKKELADFVIDNSGTIEETKKQVNDWLLKHRTV"
     gene            complement(696..1580)
                     /gene="mutM"
                     /locus_tag="PLT73_07895"
     CDS             complement(696..1580)
                     /gene="mutM"
                     /locus_tag="PLT73_07895"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRM20334.1"
                     /translation="MPELPEVETIRKGLINLVGGAVIIDVAVFWDRMITPPFSTERFK
                     TVLRGEQIHTIERRGKYLIFLLDHWAMISHLRMEGKYEVSEAGEPIKKHTHVIFYLAD
                     GRQLCYLDVRKFGKFTLLPIEKRDEYEPFKKMGPEPTADFFKLSKFKKDLSKSHRAIK
                     AVILDQNIVAGVGNIYADESLFQAKIHPLQAADTIGPAAAKRLHEAVMDVISRSVEAG
                     GSTIRTYKNTLGEAGKFQVSLAVYGQTGQPCPNCGHPIEKIRVAQRGTHYCPHCQQLP
                     KTRSKAPDITDIAYKGAK"
     assembly_gap    1747..1809
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1810..>2020)
                     /locus_tag="PLT73_07900"
     CDS             complement(<1810..>2020)
                     /locus_tag="PLT73_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRM20335.1"
                     /translation="TGYVETLFHRRRYLPDIHNRNFNLRSFAERTAINSPIQGTAADI
                     IKVAMVRMDKVLKEKGLQANMLLQVH"
BASE COUNT          442 a          524 c          432 g          559 t
ORIGIN      
        1 tccccctttt ctttcgttta ttatcgcttc attataacat acttgtggct caaaaacctt
       61 gaaaagatgg tctttatggc actttcaggc tacacagttc tatgtttgag cagccaatcg
      121 ttgacttgtt tttttgtttc ttcgattgtt ccggaattgt cgatgacaaa gtcagccagt
      181 tcttttttct tctcgatcga tagctgactt tgcatcctcc gtgtagcttc atccgcatcc
      241 aaatggttgc ggctcatcag cctttgcact tgaagttctt ctggaacata gacgaccatc
      301 acagcatcgc aactgtcctc atacccgcct acgtacagca atgggatatc cagcacaatc
      361 agtttgtgcc cttccgaaat ataacgctct ttttctgcct gtatccaatc attgatgcgc
      421 tccttcagca acacatccag ttctttacgc ttctccctat ccgaaaagat cagcgctccc
      481 aaacgtttgc gatccaacgt accatcagac agcagatagg cttccccaaa atgttcgatg
      541 atgtccgcta accctggtgc acccggaaga acgacagccc ttgctcctaa gtctgcatcg
      601 acaaccggaa tgcccgcaga caaaaacagc ttcgctacag tcgatttccc tgtagcgatg
      661 cttcctgtaa gtcctaatat atagctcatg ttcgtttatt tcgccccttt gtaggcaata
      721 tcagtgatgt ctggtgcctt tgaacgcgtc ttcggcagtt gttgacaatg cggacaataa
      781 tgcgttcctc gctgtgcaac ccttatcttt tcgatcggat gaccacagtt cggacaaggt
      841 tgcccggttt gaccgtaaac ggcaagagag acttggaatt taccggcctc tcctaacgta
      901 tttttgtagg tccggatcgt cgaccctccg gcctccactg atctactgat cacatccatg
      961 acggcttcat gcagcctttt ggctgcagcc gggccgattg tatcggcggc ttgcagggga
     1021 tggattttag cttggaaaag tgattcatcc gcataaatgt tcccgacgcc ggccacaata
     1081 ttctggtcca ggatgaccgc tttgattgcg cgatgcgact tggaaagatc ctttttgaat
     1141 ttgctcaatt tgaagaaatc cgcggtcggt tccggcccca tttttttgaa aggttcatac
     1201 tcatcccgct tttcgatcgg caacaacgta aatttaccga acttgcgcac atcaaggtaa
     1261 cacaattggc ggccatcagc gagataaaaa atgacatgcg tatgcttctt gatcggttca
     1321 ccggcttcgc tgacctcata tttgccttcc atccgcagat gggaaatcat cgcccagtgg
     1381 tccaacaaaa agatcagata cttgccgcgg cgttcgatgg tgtggatctg ctccccccgc
     1441 agaaccgtct tgaaacgttc cgtggagaaa ggcggcgtga tcatacgatc ccaaaagacg
     1501 gctacatcta tgatgacggc tcctccgaca aggttgatga gtcctttgcg gatggtttct
     1561 acttctggca attccggcat aagcaggccc ctttcattta tcggtttgtt ccttgtttat
     1621 tttgcatcat accagctatc tccggagttg ctgtccactt tgagcggcac agccaagtcg
     1681 actgcatgct ccatgatttc cggcaccaat ttttccaaga ccggaatctc ttcttccggg
     1741 cattcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnc atgcacctgc aacaacatat tcgcttgaag ccctttttcc ttaaggactt
     1861 tatccatccg gaccatcgcc actttgatga tgtcggcggc agtgccttgg atcggcgaat
     1921 tgatggccgt gcgttcggca aaggagcgca gattgaaatt gcggttatgg atatcaggca
     1981 ggtaacggcg tctgtggaac agcgtttcga cataaccggt
//
LOCUS       DAOMUJ010000340         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15794_length_2019_cov_1.671079, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000340 DAOMUJ010000000
VERSION     DAOMUJ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15794_length_2019_cov_1.671079"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLT73_07905"
     CDS             <1..313
                     /locus_tag="PLT73_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase"
                     /protein_id="HRM20336.1"
                     /translation="SDYHPLFWASKLHFFIDDVPFYNFPYTFGFLFSLGIYAQSLQQT
                     ENFEETYISLLRDTGSMTTEDLVLKHLGADITQPDFWNQGLEIMATDVKEFLRLTEKY
                     V"
     gene            complement(371..>730)
                     /locus_tag="PLT73_07910"
     CDS             complement(371..>730)
                     /locus_tag="PLT73_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRM20337.1"
                     /translation="TVDEETFTQESPLLLIALTNSIASFDRFMPQAEVDDGKMRMVVF
                     KEFSLLDSLKILPQLIMGELKNSDTVIYRTFKHATVAIADGDKLITNVDGDEGPAFPL
                     EIAILPSFIKVYGPVRS"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(741..1296)
                     /locus_tag="PLT73_07915"
                     /pseudo
     CDS             complement(741..1296)
                     /locus_tag="PLT73_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616780.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
     gene            1469..>2019
                     /locus_tag="PLT73_07920"
     CDS             1469..>2019
                     /locus_tag="PLT73_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRM20338.1"
                     /translation="MTKDYRVLLYYKYTPIEDAETFAQEHLRFCKEIGLKGRILVAPE
                     GINGTVSGTIDQTQAYIAQMNADPRFSDITYKIDETDGHAFKKMFVRPRQEIVSLHLG
                     GDDLDPNETTGEYLSPEAFREAILDENTVVIDARNDYEYDLGHFRGAVRPDIANFREL
                     PQWIRDNKEKFMEKRVVTYCTGG"
BASE COUNT          560 a          489 c          423 g          537 t
ORIGIN      
        1 gagtgactac catcccctat tctgggcgag taaattgcac tttttcatcg atgatgtgcc
       61 cttctacaat ttcccttaca cgtttggctt cctgttcagc ctgggaatct atgcacaatc
      121 gttgcagcag actgaaaatt ttgaagaaac ctatatttcg ttactgcggg atactggttc
      181 gatgacaact gaagaccttg tgctgaagca tcttggtgcc gatatcactc agccggactt
      241 ctggaatcaa ggattggaga tcatggcaac tgatgtaaag gaattcctgc gcctgaccga
      301 aaagtatgtc taaagacaaa agacaaaaca catccctgcg acctgtattt tgggtcgtag
      361 ggatgtgttt ttaagagcgt accggtccat aaacctttat aaaggaaggt agtatcgcga
      421 tttcaagcgg aaatgcaggg ccttcatcac cgtctacatt cgtgatcagt ttatcgccat
      481 ccgcaatggc gacagtagcg tgtttgaatg ttctatagat gacagtatcc gaatttttga
      541 gttcacccat gataagctga ggcaggattt tcagagaatc caacagactg aactccttga
      601 aaacgaccat tcgcattttc ccgtcatcga cttctgcttg tggcatgaaa cgatcgaaac
      661 tggcgattga attcgttaaa gcaatcagga ggagcgggga ttcctgagta aatgtttcct
      721 catccactgt nnnnnnnnnn acgattatat gatggctatt gacgaagaaa aaggcatcgg
      781 ctggttcgcc agcgctttcc ctccttcaat gaagtaagcc agtggaccta attttgtttt
      841 ttgctccaca ctcacttctt ccacagcttc aggaatcgcg ccggcagcta cattagacac
      901 aaaatatcgg tcattaactt tgccgatatc caaatccaac aagaccgcag tttcaaacat
      961 ttcgatagcc tgcccggggt ccaaagggat gccgagcgcc cttgctagat cattgactgt
     1021 tcccaatggt acgaaaccga aagagacatc tgttccggca gccgccaagc cgttgatggt
     1081 ttcattgact gtgccatcac ctcccatgca aaacacagct tcatggccgt tctttgcagc
     1141 ttcatacgca aacttcatgg catcgccctg tttccctgtt tctttgactg tcacctcttc
     1201 gaacatgttt ctcaattgcg tttccatcaa ttccagataa ctagaggcca tttcgccccc
     1261 tgatgatgga ttgacgatca atgtacaaat gcccatttca ttcccccact ttcattttat
     1321 ctctaatgta agtgtagcat gcaacactca catccataaa gtagaacacc atttttcgaa
     1381 ttttgagcat atccttggct tttcgcttac aaaatgtgta taatttcata acgttcgaag
     1441 taaaacagat tgaattaggg ggagcagtat gacaaaagac tatcgtgtcc tactctatta
     1501 taaatataca ccgatagagg atgcagaaac attcgcgcaa gaacatctac gattctgcaa
     1561 agaaatcggt ttaaaaggaa gaattttggt agcaccggaa ggcatcaacg gcacggtctc
     1621 cggaacaatc gatcaaacac aagcctacat cgctcaaatg aatgcagatc cgcgcttcag
     1681 cgacatcacc tacaaaatcg atgagacaga cggtcatgcc ttcaaaaaga tgttcgtccg
     1741 cccacgccag gaaatcgttt ccttgcattt gggaggagac gatctcgatc ccaacgaaac
     1801 tactggtgaa tacttatctc cggaagcttt ccgcgaagcc attctagacg aaaatactgt
     1861 cgtcattgat gcccgcaacg attatgagta cgaccttggc catttccgtg gcgctgtccg
     1921 tccggatatc gcaaacttcc gcgaattgcc gcaatggatc cgcgacaaca aagaaaaatt
     1981 catggaaaaa cgtgtcgtga cttattgtac cgggggcat
//
LOCUS       DAOMUJ010000341         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15907_length_2007_cov_1.985656, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000341 DAOMUJ010000000
VERSION     DAOMUJ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15907_length_2007_cov_1.985656"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLT73_07925"
     CDS             <1..583
                     /locus_tag="PLT73_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRM20339.1"
                     /translation="EPMGIKREYNFDQVGKKDMYLLHHEEIESLALNIKGIKRIRFFM
                     TFGESYLTHLKVLENVGMTSIEPIEFEGKQIVPLQFLKAVLPDPASLGPRTKGKTNIG
                     NIFTGIKDGKEKTYYIYNVCDHEECYKEVGSQAVSYTTGVPAMIGAAMVLTGQWKKPG
                     VYNIEEFNPDPFMEALNVYGLPWQEDFNPTLVD"
     gene            605..1732
                     /gene="nspC"
                     /locus_tag="PLT73_07930"
     CDS             605..1732
                     /gene="nspC"
                     /locus_tag="PLT73_07930"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRM20340.1"
                     /translation="MDFQSLPTPSYVVDETLLRRNLEILKSVKDQTGCKILLAQKAFS
                     MYHFYPLIAEYLDGTTASSVHEAQLGFEEFGKETHVFAPAFKKEEMEALLPIVDHIVF
                     NSPNQVKLYAEMVKNSPRSIEIGLRVNPELSTQDHALYDPSSPFSRLGTTAINFDESQ
                     VELLDGLHFHTLCEQGSDALEATLVAFEEKFGKYLHGMKWVNFGGGHHITKEGYDIAK
                     LVKIVNYIKEKYAVQVYLEPGEAIALNAGFLVASVLETTYNQMNLAILDTSATCHMPD
                     VLEMPYRPFIIGSGEAGEKAHTYRLGGQTCLAGDVIGDYSFDEPLQPGNRLIFTDMAI
                     YSMVKNTTFNGIQLPAIAAHTVKDGTKVIQTFGYEDFKSRL"
BASE COUNT          552 a          491 c          503 g          461 t
ORIGIN      
        1 ggaaccgatg ggaatcaaac gcgaatacaa tttcgatcag gtcggcaaga aggacatgta
       61 tttgctgcac cacgaagaaa tcgaatcttt ggctttgaac atcaaaggca tcaaacgcat
      121 ccgtttcttc atgacattcg gcgaaagcta cctgacgcat ttgaaagtgt tggagaatgt
      181 gggcatgact tccatcgagc cgatcgaatt cgaaggcaaa caaatcgtgc cgctgcaatt
      241 cctgaaagcc gtattgcctg atccagcttc attgggaccg cgcacaaaag gaaaaacgaa
      301 catcggtaac atcttcaccg gaatcaaaga cggaaaagag aagacatact acatctacaa
      361 cgtctgcgac catgaagaat gctacaaaga agtcggctcg caagccgtct cctacacaac
      421 cggtgtccca gcgatgatcg gggcagctat ggtcttgacc ggacagtgga aaaaaccagg
      481 tgtgtacaat atcgaagaat tcaatccgga tccattcatg gaagcattga acgtctacgg
      541 cttgccttgg caagaggact tcaatcctac cttggtcgac taggaggtac ggatggacac
      601 aacaatcgat tttcaatcct tgccgacacc cagctatgtg gtggatgaaa ctttattgcg
      661 ccgcaatctg gaaatcctga aatcggtcaa ggatcagaca ggttgcaaga tcttgttggc
      721 tcaaaaggct ttttcaatgt atcatttcta ccctttaatc gccgagtatc tggacggcac
      781 gaccgccagc tccgtccatg aggcacaact gggttttgag gaattcggca aagagacgca
      841 tgtcttcgcg ccggctttca agaaggaaga aatggaggcg ttgctcccga tcgtggatca
      901 catcgtcttc aattccccta accaagtgaa gctgtacgcc gaaatggtga agaacagccc
      961 gcgttcgatc gaaattggct tgcgcgtcaa tccagaactt tcgacgcagg atcatgcgct
     1021 ttatgacccg tccagcccgt tttcgcgttt gggaacgaca gccataaatt ttgacgagag
     1081 ccaagtcgaa ctgttggacg gtctgcattt ccacaccctt tgcgaacaag gttccgatgc
     1141 tttggaagcg actttggtcg cattcgagga aaaattcggg aaatatctgc acggtatgaa
     1201 atgggtgaac ttcggcggtg ggcaccacat caccaaagaa ggttacgata tcgccaaatt
     1261 ggtgaagatc gtcaactata tcaaagaaaa atatgctgtg caggtctatc ttgagcctgg
     1321 cgaagcgatc gcgctgaatg cgggattcct tgtcgcgagt gtgctcgaga cgacctacaa
     1381 ccagatgaat ctggccatcc tggatacatc cgcgacttgc cacatgccgg acgtattgga
     1441 aatgccatac cgtccgttca tcatcggttc cggtgaagcc ggtgagaagg cgcatacgta
     1501 ccgtttaggt ggacagactt gcttagcagg agacgtcatt ggggactatt ccttcgatga
     1561 gccgcttcag cccggcaatc gcctgatctt cacggatatg gccatctatt cgatggtcaa
     1621 gaacacgacc ttcaacggca tccaactgcc ggctatcgca gcccatacgg tcaaagacgg
     1681 cacgaaagtc atccagacct tcggatacga agatttcaaa tcacgactgt agaattttga
     1741 atagtggagc tgtctctgat gagacagctt ttttgctgtt ctccggcgtg gggcgcttgg
     1801 ccggagttga ggaccacatt ctgatggtct cctcctgtgg gcagaagcct cctcggagaa
     1861 gagaccaaat attaccattc tgctccggcg aaagaagcct cgccggagga acgggacatt
     1921 ttgcactgta gttttccagc taaaatatgc atatccataa aaaacaatag gactaagaag
     1981 aatttgcctc cagtgacaaa gacgata
//
LOCUS       DAOMUJ010000342         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15930_length_2005_cov_1.950256, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000342 DAOMUJ010000000
VERSION     DAOMUJ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15930_length_2005_cov_1.950256"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLT73_07935"
     CDS             complement(<1..825)
                     /locus_tag="PLT73_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM20341.1"
                     /translation="MITGFHHLTAIIKDAEKNKTFYTDILGLRLVKQTVNQDNLTDPH
                     LFYGDYKGTPGTILTFFELPRVGQRHEEDSFIHEVLLKIPQGSLPFWKKWLTSKAVRL
                     IANDEVSCSFLDPDDMKITLIEAAEVIALEQATRHSDVPAQYQIIGILGIHYTVADLG
                     TTEAFFRDVLGMSLLDHVARPISALDGESRLIQSTRLRRSHLGKGAIDHIAYSVPERK
                     DLDLILDKAKYRQITVEKIIDRGYFQSLYLREPNGLRIEIATDYPGFTLDEPLEKLG"
     gene            complement(967..1986)
                     /locus_tag="PLT73_07940"
     CDS             complement(967..1986)
                     /locus_tag="PLT73_07940"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRM20342.1"
                     /translation="MEQVLYLGSYTKRESKGVHQIILDTDKKELRDYRLIAEVDSPTY
                     LDLSADKETLYAISKTDEGGGITSFKKNENGSYDKVAEISAEGSAPCYIYFDEDKKLI
                     FTANYHGGYLTVYKENADGSFTMTDRAQHEGSSIHENQTIPHVHYSALSPDKKFLLAC
                     DLGTDEVYTYTVSDEGKLAEVARYKATPGTGPRHLVFHPNGKVAYMFGDVEVLAYDAT
                     TGTFSLLQVITTIPEEHTGFNGGAAIRISADGKFVYASNRGHDSLVVYAVSEDGETLS
                     LVEYVPAEGNIPRDFNLDPSGQFVIVAHQDSDNLTLFERDAETGKLTLLQKDVYAPEC
                     VCVFY"
BASE COUNT          512 a          481 c          474 g          538 t
ORIGIN      
        1 cccgagtttt tccaaaggtt cgtcaagtgt gaagcctgga taatcggtcg ctatttcgat
       61 gcgtaatccg tttggttccc gtaagtaaag actttggaaa taaccgcggt cgatgatttt
      121 ttcaacggtg atctgcctgt attttgcctt atccagaatc aaatcaaggt ctttgcgttc
      181 aggcacgctg taggcgatgt ggtcgatggc gcctttgcct aaatgggaac gcctcagtct
      241 agtggactgg atcagccgac tttccccatc caatgcagaa atcgggcgag caacatgatc
      301 caacaggctc atgccgagca cgtcacgaaa aaaagcctcc gttgttccga ggtcggccac
      361 agtgtaatgg atgcccagta taccgatgat ctggtattgg gcgggcacgt cgctgtggcg
      421 tgtcgcctgc tcgagtgcta tcacttcagc tgcttcaatc agagtgattt tcatgtcgtc
      481 cgggtccaag aaagaacagc ttacttcatc gtttgcaatc aaacgaacag cctttgacgt
      541 aagccatttt ttccagaacg gcagactgcc ttgggggatc ttcagcagca cttcatggat
      601 gaagctgtct tcttcgtggc gttggccgac tctgggcagc tcgaaaaaag tcaaaatcgt
      661 accaggagtt cccttgtagt ccccataaaa aaggtgggga tctgtcaaat tatcctgatt
      721 gaccgtctgc ttgacgagac gcaaccctag gatatcggtg taaaaggttt tgttcttctc
      781 cgcatctttg atgatggcgg tgagatggtg aaaaccggta atcatataag ctccaacctc
      841 cgttcaaaag atacatacaa cgagtataca ggagagtgcc tcagcgaggt aactattttg
      901 ctcagctgtg ccaataacca aaaagccccc caagttcatc cgaacgtgga gggaaatgta
      961 tgaagtctag tagaacacgc aaacgcactc aggcgcgtag acgtcctttt gcagcaacgt
     1021 caatttacct gtttctgcat cgcgctcgaa caaagtcagg ttatcggaat cctgatgagc
     1081 tacaatcacg aattggccgc tcggatccag gttgaagtca cgggggatgt ttccttcggc
     1141 agggacatat tccactaacg acaatgtttc gccgtcttcg gataccgcat agacgaccaa
     1201 tgagtcatga ccacggttgg aagcataaac gaattttccg tctgcggaaa tgcggatagc
     1261 cgcgccaccg ttgaaaccgg tatgctcttc agggatggtc gtgatgactt gcaataacga
     1321 gaacgtgcct gttgttgcat cataagccaa cacctcaaca tcaccgaaca tgtaagccac
     1381 tttaccgttt ggatggaaaa ccaagtgacg cgggcctgtc cctggagtcg ctttataacg
     1441 ggcaacttcc gccagtttgc cttcatcaga aaccgtgtag gtgtaaactt cgtcggtacc
     1501 caaatcgcaa gccaacaaga atttcttgtc tggagatagc gcgctgtaat gcacatgcgg
     1561 aattgtttga ttttcatgga tgctcgaacc ctcatgttgt gcgcggtctg tcattgtgaa
     1621 tgaaccatca gcgttctctt tgtagacagt caagtaacca ccgtggtaat tggcagtgaa
     1681 gatcaatttt ttgtcttcgt cgaagtagat atagcaaggc gcagatcctt cagcagaaat
     1741 ttcagccacc ttgtcatagc ttccgttttc attttttttg aaagaagtga tgccaccacc
     1801 ttcatccgtt ttggaaatcg cgtacagcgt ttctttgtcc gcagataagt ccagataagt
     1861 agggctgtcg acttccgcaa tcaagcgata atcacgtaat tcttttttat cggtgtccaa
     1921 aatgatttga tgaacccctt tactctctct ttttgtgtaa gagccaagat ataaaacttg
     1981 ttccatccaa agacctccta aatat
//
LOCUS       DAOMUJ010000343         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15945_length_2004_cov_2.407901, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000343 DAOMUJ010000000
VERSION     DAOMUJ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15945_length_2004_cov_2.407901"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLT73_07945"
     CDS             complement(<1..591)
                     /locus_tag="PLT73_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRM20343.1"
                     /translation="MTLPLEFIRRGVADHADRTAIYFGNESLTFSQANDTANRIAGGF
                     AEAGLKKGDHVGLLYGNSLWTIPVDFATMKSGLVRIPLNPRLSLEEHTRMLVEADVRV
                     LVHDAATRDHAAALLKGIPNGRALGLGCPAADGRGPDLLDAKYAGAGEPDVEISGDDP
                     LMLIYTSGTTGKLKAVIHTHRNFGAIGDNILANLMSP"
     gene            complement(649..1446)
                     /locus_tag="PLT73_07950"
     CDS             complement(649..1446)
                     /locus_tag="PLT73_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM20344.1"
                     /translation="MHPQHYFLDTWSYATNTAKKTVESGGNTWFILSVDYELGNALTK
                     DITKAVTDSGGKIVGSVRHPINTQDFSSFLLQAQASKANIIALANAGTDNMNAIKQSA
                     EFGIMQGGQNLAGIFTNLPNVKALGLHAAQGMYLSTTWYWDRNEATRAFAKELAKRNK
                     GIYPEAPHAGIYSALIHYAKAVEKIHTDNGTRVVAAMKQMSTDDPLFGPGRIREDGRK
                     IHPIYLMQVKSPAESKHEYDFYKTISTTPAEQAFQPMEEGNCRLVSK"
     gene            complement(1380..1850)
                     /locus_tag="PLT73_07955"
     CDS             complement(1380..1850)
                     /locus_tag="PLT73_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM20345.1"
                     /translation="MNIKYKYMVLALGALPLAAAAQFTDNKIKIGILTDMSSMYADFS
                     GQGSVEAAKMAVEYLGGKIDGKPVEIVFADHQNKVDIGLTVARKWFDVEGVDLIMDVP
                     QSAVALAVADLAHQRGKAAIFSSAGTSTLTDSKCTPNTISWTLGLMPPIRPRRP"
BASE COUNT          398 a          540 c          576 g          490 t
ORIGIN      
        1 cgggctcatc aggttggcca ggatgttgtc gccgatcgcg ccgaaattgc gatgggtatg
       61 gatgaccgcc ttgagcttgc ccgtcgtgcc cgaggtgtaa atcagcatga gcgggtcatc
      121 gccgctgatc tcgacatcgg gttcacctgc gccggcgtac ttcgcatcca gcaagtcggg
      181 cccgcggccg tcggctgccg ggcaacccag tcccagcgcc cggccattgg gaatgccctt
      241 gagcaacgcg gccgcgtggt cgcgggtagc cgcgtcgtgc acgaggacgc gcacatcggc
      301 ttcgaccaac atgcgcgtat gctcttccaa ggaaagccgg gggttcagtg gaatgcggac
      361 cagcccgctt ttcatggtgg cgaaatcgac gggaatcgtc cagagactgt ttccgtaaag
      421 cagcccgacg tggtcgcctt ttttcaagcc ggcttctgca aagcccccgg cgatgcggtt
      481 ggccgtgtcg ttggcttggg agaacgtcaa tgattcgttg ccaaaataga tcgcggtgcg
      541 atctgcgtgg tcggccacgc cacgacgaat gaactccagg ggtaatgtca tgatgactgc
      601 ctttgcttat aaatggcgat attgagtgat acggttatcc gcgactgact acttggagac
      661 gagccggcaa ttgccttcct ccatgggttg aaaggcctgc tccgcggggg ttgtgctgat
      721 ggtcttgtag aagtcatatt catgcttgga ttcagcgggt gacttcactt gcatcagata
      781 gatcgggtgg atttttcggc catcttcccg aatgcggccc ggaccaaata gcggatcatc
      841 agttgacatt tgcttcatcg cagctacgac cctggtgccg ttgtccgtat gtattttttc
      901 aacggccttc gcgtaatgga tcagcgcgga atagatgccg gcatgcggtg cctcgggata
      961 aattcccttg ttgcgcttgg caagctcttt ggcgaacgcc cgggttgcct cgttgcgatc
     1021 ccaataccag gtcgtgctca aatacatgcc ttgagcggca tggaggccaa gcgctttcac
     1081 attggggaga ttggtgaata taccggcaag attctgtcca ccctgcatga tgccgaactc
     1141 cgcgctttgt ttgatagcat tcatgttgtc cgtgcccgca ttcgccaagg cgatgatgtt
     1201 tgctttggaa gcctgggctt gcaacaggaa agatgaaaaa tcctgggtat tgatggggtg
     1261 ccgaaccgag ccgacgatct tgccgccgct gtctgtcact gcttttgtga tgtctttggt
     1321 cagggcattg cccaattcat agtcgacact caagatgaac cacgtgttgc caccactttc
     1381 tacggtcttc ttggccgtat tggtggcata agaccaagtg tccaggaaat agtgttgggg
     1441 gtgcacttgc tatcggtcag tgtcgaggtg cctgcggacg agaaaatggc tgccttgccg
     1501 cgttgatggg cgagatcggc cacagccagt gccaccgctg actggggcac gtccatgatc
     1561 aggtccaccc cttcgacatc gaaccacttg cgtgccacag tgaggccgat atccacctta
     1621 ttttgatgat cggcaaatac gatttcaacc ggcttgccat cgatcttgcc acccagatac
     1681 tcgacggcca ttttcgccgc ttccaccgag ccctgaccag aaaagtcggc gtacatgctc
     1741 gacatgtcgg tcaaaatgcc tatcttaatt ttattgtcgg taaattgagc ggcggccgcc
     1801 aagggtagtg cacccaatgc gagaaccata tatttatatt tgatattcat actttctgtc
     1861 tcctgtagtt aatgattcga agccctgttt gcccaacaac ggtcgtatcc atcggtggat
     1921 gggccaagcg gtcactgcgt gaagcgatag gcccatcgat caatccgggg cgttcctgcc
     1981 ttgcccgggt cacaggggtg tgat
//
LOCUS       DAOMUJ010000344         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15966_length_2002_cov_1.663071, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000344 DAOMUJ010000000
VERSION     DAOMUJ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15966_length_2002_cov_1.663071"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="PLT73_07960"
     CDS             complement(<1..118)
                     /locus_tag="PLT73_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRM20346.1"
                     /translation="MQIGILTFKEPENDKSRKIIHVDMDAFYASVELLRYPQL"
     gene            complement(144..758)
                     /locus_tag="PLT73_07965"
     CDS             complement(144..758)
                     /locus_tag="PLT73_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRM20347.1"
                     /translation="MTPHKEDYLKVIMELGGDKQLVNNKQIGQEMSVKLLKDGLISHI
                     PYQGVQITEKGQLIANKLIRKHRLWEVFLSEKLDFNWDEVHEEAELLEHVSSDRLVDR
                     LDAYLGFPKYDPHGGVIPDKEGRIEVYESKPLTELEVGTRFIIREVDDDQEFLAYLLQ
                     KNVKLSVPYLLTNVEPYEGPYSFETQNHQSHQISFKAASKIYVQ"
     gene            complement(1025..>2002)
                     /locus_tag="PLT73_07970"
     CDS             complement(1025..>2002)
                     /locus_tag="PLT73_07970"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM20348.1"
                     /translation="TIIAIGGGSPMDAAKAMWLFYEHPESDFFGAKQKYLDIRKRTYK
                     IKDMEKAKLVCIPTTSGTGSEVTPFAVITDSETHIKYPLADYALTPDIAIVDPQFVYS
                     VPKSVTADTGMDVLTHAIESFVSVLANDYTKGLSLQAIKLVFENLRNSYNYGDQESRD
                     AKAREQMATASTMAGMAFANAFLGISHSIAHKIGGQWDLIHGRTNAILLPHIVRYNAI
                     DPQKHALWAKYEYFRADEDYAEIARYLGFKGNTTAELVEALATEITKLGQDIGIKMNF
                     REQGITEEMLERDADRLAELAFEDQCTTANPKQPLISELKEIIYAAYKG"
BASE COUNT          500 a          455 c          424 g          623 t
ORIGIN      
        1 ttaattgcgg atagcgcagc agctcgaccg atgcgtagaa ggcatccatg tccacatgga
       61 tgatcttcct gcttttatca ttttcgggct ccttaaatgt caagatccca atctgcatac
      121 tgttctcctt actttagccg gattcattgc acataaattt tacttgctgc tttgaaactg
      181 atttggtggg actgatgatt ctgcgtctca aagctataag gaccctcata cggctccaca
      241 tttgtcagca gataaggtac gcttaacttt acattctttt ggagcaaata cgccagaaat
      301 tcttgatcgt catccacttc cctgatgata aagcgggtac cgacttccaa ttcggtcaat
      361 ggtttgcttt cataaacctc aatccgccct tctttgtcgg ggattacacc tccatgcgga
      421 tcatatttag gaaatcctaa ataagcatcc agtctgtcga cgagtcgatc tgaggataca
      481 tgctcaagca gttcggcctc ttcgtgcact tcgtcccaat taaaatccaa tttttccgag
      541 aggaatactt cccaaagtcg atgtttccga atcagcttat ttgctatcaa ttgtcccttt
      601 tcggtgatct gcacaccctg atacgggatg tggctgatca ggccatcctt gagtaatttg
      661 actgacattt cctgcccaat ttgtttatta ttgacaagct gtttgtctcc acctaattcc
      721 atgattactt tcaaataatc ttctttgtgc ggcgtcatac gccccctcct ttttaggtta
      781 acctaaacta tatttcatat tatacgacta attggatcga ttgcaaggct aaaacttaaa
      841 ataagccctt ccatttcagt atatattagg cttgaaaacc tagtaaggat gatggacgac
      901 aaatcagcgg aacccattgg gcaagatgga caagccctta tatttcgacg gattccgtaa
      961 tgccaagtta ttttttgtaa gcaaaaaagc cgggagcgcg tcccggcttt ttcattatat
     1021 tctcttaacc tttgtaggct gcgtagatga tttctttcaa ttctgagatt aatggttgtt
     1081 tagggttagc tgttgtacat tggtcttcaa atgccaattc agctaagcgg tctgcatcgc
     1141 gttcaagcat ttcttcagtg atgccttgtt cacggaagtt catcttgatg ccgatgtctt
     1201 gacctaattt agtgatttca gtcgccaaag cttcgaccaa ttcagcagtc gtattgcctt
     1261 tgaaacctag gtagcgagcg atttcagcgt agtcttcgtc tgcacggaag tactcatatt
     1321 tagcccacaa cgcatgtttt tgcggatcga tcgcattgta gcggacgata tgcggcaaca
     1381 ggatagcgtt cgtacggccg tggatcagat cccattgacc gccgattttg tgtgcgatcg
     1441 agtgggaaat acccaggaac gcattcgcga aggccatacc agccatagta gaagcggtag
     1501 ccatctgttc tcttgccttg gcatcgcgtg attcttggtc gccgtaattg tatgaatttc
     1561 tcaagttttc gaataccaat ttgattgctt gtaaactcaa acctttagtg tagtcgtttg
     1621 ccaatacgga aacaaaagat tcgatggcat gcgtcaatac gtccataccg gtatctgcag
     1681 ttacagattt aggaacactg tatacgaatt gtggatccac gatagcaata tcaggagtca
     1741 atgcgtaatc agccaatggg tatttgatgt gtgtttcgct gtctgtgata accgcgaatg
     1801 gcgttacttc cgaaccagta cctgaggtag ttgggatgca gacaagtttc gctttttcca
     1861 tatccttgat cttgtaagta cgtttgcgga tatccaagta tttttgttta gcgccgaaga
     1921 agtcactttc tgggtgctca tagaacaacc acatcgcttt ggcagcatcc ataggagaac
     1981 ccccgccgat tgcgatgatt gt
//
LOCUS       DAOMUJ010000345         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_15999_length_1999_cov_2.361626, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000345 DAOMUJ010000000
VERSION     DAOMUJ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15999_length_1999_cov_2.361626"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLT73_07975"
     CDS             <1..181
                     /locus_tag="PLT73_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20349.1"
                     /translation="QLLVKYPAVKISEESIPNKAGFILQEGGVDYNYCFDDLIEDVKN
                     DFAAELATLAFQDEK"
     gene            194..1201
                     /locus_tag="PLT73_07980"
     CDS             194..1201
                     /locus_tag="PLT73_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRM20350.1"
                     /translation="MQEIDYKGVNTILRTYELKLLAEADYDRMLKASSLKDALDVLKG
                     TGYEFDEKETLMNKNFEGFLIKHLAEVYQDLFSITPVAEIVELFALRYTYHNLKVLLK
                     QRFSGEDLEQLLIPIGKYSIASLKKLVETGDGNDEHPILIEGVQEALRDFDEGHRIEA
                     ATIFMDTYYFRHLRSISDKLQHPSIAKMVDGMIDLYNLSTIIRSLKQNKPRGFLYTVL
                     SSSGSISKRDLIEESINGPSAVMRKHYFSKEYGARLSNMISDTTNEVDTQKLDKLVDE
                     LTYELIAEGLYQPFGPIPLLGYLFAKEKEVTNIRLILVGKDNQIDEQILRERMRPIYG
                     S"
     gene            1191..1502
                     /locus_tag="PLT73_07985"
     CDS             1191..1502
                     /locus_tag="PLT73_07985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRM20351.1"
                     /translation="MAHNIAVVGDKDSILPFKILGFAVFACHNAQTARETVDRLAMEN
                     YGIIYLTEEMAKEIPETVSRYDSRVQPAIILIPNHKGTLNIGKTRIQENVEKAVGQNI
                     L"
     gene            1519..>1999
                     /locus_tag="PLT73_07990"
     CDS             1519..>1999
                     /locus_tag="PLT73_07990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002317.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the A subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM20352.1"
                     /translation="MKNGNIIKVSGPLVMAEGMEEANIQDICRVGELGLIGEIIEMRN
                     DVASIQVYEETSGIGPGEPVITTGEALSVELGPGILSQMFDGIQRPLDTFREITQSNY
                     LVRGTDIPSLKRENVWEFKATKKVGDYVVAGDIVGTVQETKVIEHRIMVPYGVSGKLT
                     "
BASE COUNT          603 a          385 c          495 g          516 t
ORIGIN      
        1 gcagctgttg gtcaaatatc cagctgtcaa gatttctgag gagtccattc cgaataaagc
       61 cggattcatc ctgcaggaag gcggagtcga ttacaactat tgcttcgatg atctgatcga
      121 agatgtaaag aacgactttg cagcagagct ggcgacttta gcattccaag acgaaaagta
      181 aaggaggggc actatgcaag agattgatta caaaggcgtc aatacgatac ttcgcaccta
      241 tgaattaaaa ttattggcgg aagcggatta cgatcggatg ctgaaagcct cttctctaaa
      301 agatgccttg gatgttctga aaggtaccgg ttatgagttt gatgagaaag aaacactgat
      361 gaataaaaat tttgaaggtt ttctcataaa acacctggcg gaagtctatc aggacttgtt
      421 cagcatcacg ccggttgctg agattgtgga attattcgcg ttgcgctata cctatcacaa
      481 cctgaaagtg ttgctgaaac aacgtttttc gggtgaagat ctggagcagc tgttgatccc
      541 tatcggcaaa tattccatag ccagtctgaa gaaattggtc gaaaccggcg acggcaacga
      601 cgaacatccg attctgatag aaggggttca agaagcgctc cgcgatttcg atgaagggca
      661 tcgtatcgaa gcggctacta tctttatgga cacttattat ttcaggcatc tgcgttccat
      721 cagcgacaaa cttcaacatc cgagcatcgc gaaaatggta gatggcatga tcgatctcta
      781 taacctttcg accattatcc gaagtctgaa acaaaataag ccccgcggtt ttctctatac
      841 ggttttatcc agttcaggtt caatttcgaa acgcgatttg attgaagaat cgatcaacgg
      901 accaagcgcg gtcatgcgca agcattattt cagcaaagaa tacggcgcgc gcttatccaa
      961 tatgatttcc gatacaacaa atgaagtaga cacacagaag ctggataaac tcgttgatga
     1021 actgacctat gaattgattg cggaaggctt gtaccaaccg ttcggtccga ttcctttgtt
     1081 gggttatctg tttgccaaag agaaggaagt cactaatatc cgcttgattt tggtaggcaa
     1141 agacaatcag atagatgaac aaatattgag agaaaggatg cgacctatat atggctcata
     1201 atattgcagt tgtgggagac aaagattcca tccttccttt taagattttg ggtttcgctg
     1261 tatttgcatg ccataatgcc cagactgccc gtgaaacggt agatcgtctg gcgatggaga
     1321 attatgggat catttatctg acggaagaaa tggcaaaaga aatacctgag acagtgagca
     1381 ggtatgacag cagagtccaa ccagccatca ttctgattcc gaaccataaa ggtacgttga
     1441 atatcggaaa aacccgcatt caggagaatg tagaaaaagc agttggacag aatatcttat
     1501 aatttggagg ggactttttt gaaaaatgga aatataataa aagtttccgg acctttagtt
     1561 atggcggaag gaatggaaga agcgaatatc caagatattt gccgcgtcgg cgaattagga
     1621 ttgatcggtg aaatcatcga gatgcgcaat gatgttgcat ctattcaagt ttacgaggag
     1681 acttccggta ttggtccagg agaaccggta atcacgacag gagaggcttt atctgttgaa
     1741 ttgggaccag gtatcctgtc gcagatgttt gacgggattc agcgtccttt ggatacgttc
     1801 cgggaaatca cccaaagtaa ttatttggtg cgtggaacgg atattccatc actgaaaaga
     1861 gagaacgtct gggaattcaa agctacgaag aaagtcggcg attatgtcgt agcaggggat
     1921 attgtcggta ccgtgcaaga aacgaaagtg atcgagcacc gaatcatggt tccgtatggc
     1981 gtctccggta agctgactg
//
LOCUS       DAOMUJ010000346         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16080_length_1993_cov_1.390609, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000346 DAOMUJ010000000
VERSION     DAOMUJ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16080_length_1993_cov_1.390609"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLT73_07995"
     CDS             complement(<1..749)
                     /locus_tag="PLT73_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20353.1"
                     /translation="MKKSKALQAEANKKENVTEAYNKAFMAEIQPKGGLVFEDKYVKK
                     ADGYETCLHVYDMKTTQNTFWLNQLMQRDEVIAVMDVLTAERDDILKTIDRSIDEFYA
                     RALEEKSNIEQLESQMSISTLQRVANEISQRGESIKIVVNRLYLSAATREGLDKKINR
                     LKGELTGLGYKTTILLTGMKEEWDGLFLPLDKQMESNFTINGKYIPARSLGGGYPFHH
                     KSLEDPFGMYLGRTSTNGVVLFDRYMNDNRNR"
     gene            complement(764..1345)
                     /locus_tag="PLT73_08000"
     CDS             complement(764..1345)
                     /locus_tag="PLT73_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20354.1"
                     /translation="MQTNGKSAWENIKSLFQSPNGGIKEMADWIPYIDLTAEGYLVMR
                     DRRYQNMVQIESHDLNTLTESDKNRLIEEFSRFLTSTNFPQKYLSIRSNVDTSDQRNY
                     WAKMYRNAKNDLQKKRILHTIEQLKFVEENATNQEYYLLFSATTIQELQRAENEIYIF
                     GGQALGAKPVPVEKKLKILDRLMNMSSYAEGEE"
     gene            complement(1345..1674)
                     /locus_tag="PLT73_08005"
     CDS             complement(1345..1674)
                     /locus_tag="PLT73_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20355.1"
                     /translation="MYEIPKELKTDIKLWAVYSSDIPFLLMMLLLTLMLEDFFTGGIF
                     RLKLAYYAVNVLFTFLFITKPVPNISIFKLITHGLLQDFRKVKSLDYQKYAAVAHLEE
                     WKEEEAN"
BASE COUNT          503 a          475 c          369 g          646 t
ORIGIN      
        1 cgattccggt tgtcgttcat atagcgatca aatagaacga cgccattcgt gctggtacgt
       61 cccaaataca tgccaaaggg atcttccaag cttttatggt gaaacggata gccgcctcct
      121 aaagaacgcg ccgggatata cttgccgttg atcgtaaaat tactctccat ctgcttatcc
      181 aaggggagaa acaaaccgtc ccattcttcc ttcatacccg tcaaaagaat ggttgtttta
      241 taaccaagcc cggtcaactc cccttttaac cgattgattt tcttgtccaa tccttctcgc
      301 gtggctgctg acaaatacaa gcgattcacc acaattttga tcgattctcc tctttgtgaa
      361 atttcattgg ccacgcgctg caaggtggaa atgctcattt gcgattccaa ttgttcaatg
      421 ttactttttt cttccaaagc gcgggcgtaa aattcgtcta tggaccgatc aatcgttttt
      481 aaaatatcgt cacgttccgc cgtcaacacg tccattaccg caatcacttc gtctcgttgc
      541 ataagttgat ttaaccaaaa ggtgttctgg gttgttttca tatcgtatac atgcagacac
      601 gtttcatagc catctgcttt tttgacgtac ttatcttcaa aaacaagacc gccttttggc
      661 tgaatttcgg ccataaatgc tttgttataa gcttcagtta cattctcttt tttatttgct
      721 tctgcttgaa gggcttttga ctttttcatt gtccgttcca tgttcactcc tccccttccg
      781 cataactact catattcatt agccgatcta atatcttcaa ctttttttca accgggaccg
      841 gtttggcgcc caatgcttga ccaccaaaaa tatagatttc attttcagct cgctgcagct
      901 cctggatcgt ggtggctgaa aaaagtaaat agtattcctg gttcgttgca ttttcttcta
      961 caaatttgag ttgttcgatg gtgtgtaaga tccgcttttt ctgtaagtca ttttttgcat
     1021 tccgatacat tttggcccaa taattgcgct gatcggacgt gtctacattg ctccgaatac
     1081 tcaaatactt ttgcggaaaa ttcgtactcg tcaaaaaacg ggaaaattcc tcgatcaatc
     1141 ggtttttatc actctcagtc aatgtgttta aatcatggga ttcaatttga accatgttct
     1201 gatatcgtct atcccgcatc acaagatagc cttcggcagt cagatcaatg taagggatcc
     1261 aatcggccat ctctttgatc cccccattcg ggctttgaaa caacgatttg atattctccc
     1321 aagcagattt gccgtttgtt tgcattagtt cgcctcctct tccttccact cttctaaatg
     1381 agcgactgct gcatattttt gataatccaa actttttact ttccgaaagt cttgcagaag
     1441 cccatgggtg atcagcttaa aaatcgaaat attcgggacc ggtttggtaa taaacagaaa
     1501 agtgaacagg acgttcacgg cataataggc tagctttaaa cggaagattc cgcccgtaaa
     1561 aaaatcttcc aacatcagcg tcagtagcaa catcatcaat aaaaaaggaa tgtcactgga
     1621 gtaaaccgcc cataacttga tatcggtttt caactctttt ggaatttcat acatctttgt
     1681 tcctcctaac cagaaaagga agcggaattg cataaagctt gcaataaccc cttcctccct
     1741 ttattttcgg ttcccatttc gattctgaat gtctttcgtc ggttttatcg aatcttccat
     1801 tcgtttctga cgcggttgta ttcgcccgtc tattgcgcga gtttccagaa tggattctga
     1861 gatcggggta cttggaccgg agttctcttg ttgtaacacg taccgcaaag ccggctttga
     1921 atctgtttgt actgaagatc tgtttactat cacggcttct cgcataggaa cggaccttct
     1981 gacagaagaa tcc
//
LOCUS       DAOMUJ010000347         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16127_length_1989_cov_1.791624, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000347 DAOMUJ010000000
VERSION     DAOMUJ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16127_length_1989_cov_1.791624"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLT73_08010"
     CDS             complement(<1..1112)
                     /locus_tag="PLT73_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRM20356.1"
                     /translation="MQEKSLNIIAGAVVIATLASLLLHYWSNASEFTYPEAERIRTAA
                     LQLDPNDMRINGDLLTGEALLQSEDKEETVYFYYTIETQEEKFNWEQQRFPQQVVATV
                     RLEEPEAERNLHQFDFEDYLNAKSIHWVVTVEAMQFRSEDRSFRAWPSSIRRSIILML
                     EKLPAVQTTDYIQTMLFNQSHAIAGDTLDAYRGIGLLHLFSISGMHIQLLLTQARFIL
                     LRMKVSHETTNKLLVVFLIAYGVLTGWGIGVFRAICTHFILLMGKITGHRIEAKDAFA
                     LTVMIVVLYNPLLIYSASFQLSYLLAGVLYFVAPVSSEWEMNGLLKDVCLTALMTLAS
                     FPVVAYHFFEVSWLGIFVNVIFSFFFSWLLCRPCMC"
     assembly_gap    1322..1331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1332..1764)
                     /locus_tag="PLT73_08015"
     CDS             complement(<1332..1764)
                     /locus_tag="PLT73_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HRM20357.1"
                     /translation="MERIPWNQYFMAQSVLLSLRSTCKRLEVGATIVRDKRIIAGGYN
                     GSVSGDVHCIDEGCYVVNGHCVRTIHAEMNAILQCAKFGAKTEDAEIYVTHFPCLQCT
                     KMILQAGIKKIYYLEDYHNDSYAIHLLDAMDIPYEQVPLDPD"
     gene            complement(1828..1989)
                     /locus_tag="PLT73_08020"
                     /pseudo
     CDS             complement(1828..1989)
                     /locus_tag="PLT73_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722511.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
BASE COUNT          572 a          502 c          410 g          495 t
ORIGIN      
        1 caacacatac aaggtctaca aagcaaccaa gaaaaaaaga acgaaaagat cacattcaca
       61 aaaatcccga gccacgaaac ctcaaaaaaa tgataggcga cgaccggaaa ggacgccaat
      121 gtcatcagtg ccgtcaaaca gacgtccttc aatagcccgt tcatttccca ttccgagctg
      181 accggagcca cgaaatacaa tactccggcc aaaagatagc tcaattgaaa acttgccgaa
      241 taaatcagca gcggattgta taaaacaaca atcatcaccg taagcgcaaa ggcatccttc
      301 gcttcaatcc gatgcccagt gattttcccc atcaaaagaa taaaatgcgt gcagatagcc
      361 cggaagacac cgattcccca tccggtcaat acgccataag cgatcagaaa cacaaccagc
      421 agcttatttg tggtctcgtg gctgactttc atccgcagca ggataaagcg cgcctgggtc
      481 aacaagagct ggatatgcat accggagatt gaaaaaagat gcaataaacc gatcccgcga
      541 taggcatcca aagtgtcccc cgcaatagca tgcgactgat tgaataacat ggtttggatg
      601 tagtcggtcg tctggacagc aggcaacttt tccaacatca agatgatgga tctcctgatg
      661 ctgcttggcc aggcccggaa agaacgatcc tcgctccgga actgcatcgc ttcgaccgtg
      721 acgacccaat ggatgctttt cgcattcaga taatcctcaa aatcaaattg gtgcagattc
      781 cgttcagctt ccggctcctc cagccggact gtggcaacga cctgctgggg aaatcgctgt
      841 tgttcccaat tgaacttctc ctcttgcgtt tcgatcgtgt aatagaaata aacggtttct
      901 tccttgtctt ccgattgcag caaggcttcc cctgtcaaca aatcgccgtt gatccgcata
      961 tcgttcgggt ccaactgcag cgcagccgtt ctgattctct ccgcttccgg ataagtgaac
     1021 tcagatgcat ttgaccagta atggagcaac aagcttgcaa gtgttgcgat cacaaccgcg
     1081 ccagcaataa tattcagact cttttcttgc ataaaaatga gtcgaacaaa cataacgatt
     1141 agcagtaacc atgcaaaata attgtttttg tcgaacccaa taatagttat cgggaagacg
     1201 agcaaggcag gcaaaagcag atagccttta atggtcagtc atgatgaccg caacaagcag
     1261 cagagtcatc atcttgttga tcttttcgtt tcagtaattg ataaaaatac agtagataca
     1321 cnnnnnnnnn naatccggat cgagggggac ttgttcataa ggaatatcca ttgcatccaa
     1381 aagatgaatg gcataggagt cattgtgata gtcttccaaa tagtagattt tcttgatccc
     1441 agcctgcaaa atcatcttcg tgcattgtag acaaggaaaa tgcgtcacat atatttccgc
     1501 atcctctgtc ttggcgccga atttggcgca ctggaggatt gcattcattt ccgcgtggat
     1561 agtgcggaca caatgcccat tcacgacata gcagccctca tcgatacagt gcacatcgcc
     1621 ggaaacactc ccgttgtatc ctccggctat gatgcgcttg tccctgacga ttgtggctcc
     1681 gacctccaag cgtttacatg tgctccgcag cgagagcaat acgctttgtg ccataaaata
     1741 ttgattccaa ggaattcttt ccatgcagta gttacctcca tccatatata aaattaagta
     1801 tacaagagct gctctcttga ttcaatttta ttttgcgccg accgtgatta gatcttttaa
     1861 cccctcgaag gttttttcac caattcctga aacttccatt atatcctcga tcgtctggaa
     1921 agagccgttc tcttcacggt acgcgatgat gagcgcggct ttttttcacc aatgccattc
     1981 aatgtttgc
//
LOCUS       DAOMUJ010000348         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16148_length_1987_cov_2.391822, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000348 DAOMUJ010000000
VERSION     DAOMUJ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16148_length_1987_cov_2.391822"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>217)
                     /locus_tag="PLT73_08025"
     CDS             complement(<1..>217)
                     /locus_tag="PLT73_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20358.1"
                     /translation="QKLEQLEGQKQIFQQRAMYSSKNQEEQAQIIAEKEALIKTEKAN
                     LAALRTELAAKVEVRKNLSGKRASLSEK"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<228..>560)
                     /locus_tag="PLT73_08030"
     CDS             complement(<228..>560)
                     /locus_tag="PLT73_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20359.1"
                     /translation="KTSAERKLSQTEEHLNRVEDILHEINNQLAPLEIQRKTALDFQE
                     KKTLLSEIEIALTASEIERLNAQWQMSKRELADYDRKISMEEQSLAVTQAALKKLKQK
                     LDACDEQL"
     assembly_gap    561..570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<571..1049)
                     /locus_tag="PLT73_08035"
     CDS             complement(<571..1049)
                     /locus_tag="PLT73_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20360.1"
                     /translation="MSGFKSFADKTVIEFDEGVTAVVGPNGSGKSNLSEAVRWVLGEQ
                     SAKNLRGKKMDDIVFAGSQTRKPVNIAEVTLILNNEDGFLPLEFSEVSITRRYNRNGD
                     SDCFINKKPCRLKDITELLMDSGIGKDSFSMISQGKVEQIFQNKPEDRRVIFEEAAGV
                     "
     gene            complement(1102..1791)
                     /gene="rnc"
                     /locus_tag="PLT73_08040"
     CDS             complement(1102..1791)
                     /gene="rnc"
                     /locus_tag="PLT73_08040"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRM20361.1"
                     /translation="MEKIVKQIATDFSIVFRNEKLLHEAFTHSSYVNEHRHLALQDNE
                     RLEFLGDAVLEVIISDYLFHTYDTWHEGKMTRLRAQIVCEPSLSAFAKDCHFDSYIRL
                     GKGEENMGGRQRPALLCDVFEAFIGAVYLDQGIEAARKFISQVMITKLNEDAFSHVMD
                     YKTGLQEELQKNGDVDIQYILLSEDGPAHAKQFDVEVRAFGKVMGQGQGPSKKAAEQK
                     AAQNALESLKK"
BASE COUNT          453 a          485 c          389 g          640 t
ORIGIN      
        1 ccttttcgga aaggctcgct ctttttccag agagattttt ccttacttcc actttcgccg
       61 caagttcagt ccgtaaagct gccagattgg ctttttcggt cttgatcagt gcctcttttt
      121 cagcgatgat ttgggcttgc tcttcttgat tcttagatga gtacattgcc cgttgctgga
      181 aaatctgctt ttgaccttcc agctgttcca atttctgnnn nnnnnnntcc aactgttcgt
      241 cgcaagcatc cagcttttgc ttgagtttct tcaaggcagc ctgcgtgacc gcaagcgatt
      301 gttcttccat gctgattttt cggtcataat cagccagctc gcgtttgctc atttgccact
      361 gcgcattcag cctctcaatt tcagaagcgg tcaaagcaat ttcaatctcg cttaataacg
      421 tttttttctc ctggaaatcc aaagctgttt ttctttggat ttccaaagga gcgagttggt
      481 tgttgatttc atgcaggatg tcctcaaccc gattcagatg ctcttccgtt tgggacagct
      541 ttcgttcagc actcgtttta nnnnnnnnnn actccggcag cctcttcgaa tatgactctt
      601 ctgtcctcag gtttgttctg aaaaatctgt tccactttcc cttgcgaaat catggaaaaa
      661 gaatcttttc cgatgcctga atccatcaat aattccgtta tgtctttcaa tcggcatggc
      721 tttttattga tgaaacagtc actgtcgcca ttgcggttgt atcttctggt gatgcttact
      781 tcgctgaatt ccagcggcag aaatccgtcc tcgttgttca ggatgagggt aacttcggca
      841 atgttgacag gcttgcgtgt ctgcgacccg gcaaacacga tatcatccat ttttttgccg
      901 cgcagatttt ttgcggattg ttcccccaat acccaacgga cagcttcaga aaggttgctt
      961 ttgccgcttc cgttcggtcc tactaccgct gtcacacctt catcaaattc gattacggtt
     1021 ttgtcggcga atgatttgaa acccgacatt tctattcttt caagatgcaa agaaaacatc
     1081 ctttccggtg aaacttgctt ttcatttttt tagagattcc aaagcgtttt gtgccgcttt
     1141 ttgttcagct gcttttttgc taggaccttg accttgaccc atcacttttc cgaatgcccg
     1201 cacttccaca tcgaactgtt ttgcatgtgc gggcccgtct tcactcagaa gaatatattg
     1261 gatatccaca tccccgtttt tttgtagttc ttcttggagg ccggtcttat aatccatcac
     1321 atgtgaaaaa gcatcctcgt ttaacttggt gatcatgact tggctaatga actttctggc
     1381 ggcttcgatt ccttgatcaa ggtaaacggc cccaatgaac gcttcgaata catcgcacag
     1441 caacgctggt cgttgccttc cgcccatatt ctcttcccct ttacccaaac ggatataaga
     1501 gtcgaagtga caatccttcg cgaaagcaga taaactaggt tcgcagacga tttgcgcccg
     1561 cagacgggtc attttgcctt catgccacgt gtcataagta tgaaacaaat aatcggagat
     1621 gatgacttcc aggaccgcgt ctcccagaaa ttccaaccgc tcattgtcct gcaatgccag
     1681 atgacgatgc tcattcacat aagatgaatg ggtaaatgct tcatgcaaaa gtttctcgtt
     1741 cctgaaaaca atggagaaat cagttgctat ttgtttgact attttttcca attcattccc
     1801 tcctttcctt gaatggactc attattatca aagtactttt gaaaatagcg tcctttttga
     1861 ttatacgaca aatgtgtact attagcacga aaatttagtt tccggaaagt ccacccgtaa
     1921 ctaagaaaag ctgaacgggt tcgttcagcc ctgattaagg aaacactgtg acctaatcgg
     1981 tcaagtg
//
LOCUS       DAOMUJ010000349         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16160_length_1986_cov_2.676334, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000349 DAOMUJ010000000
VERSION     DAOMUJ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16160_length_1986_cov_2.676334"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1010
                     /locus_tag="PLT73_08045"
     CDS             165..1010
                     /locus_tag="PLT73_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM20362.1"
                     /translation="MDKKIIFLDIDGTLVTDDGWVPSSAADACRQARLNGHEIYLCTG
                     RSKPEIYDFIMEIGFDGIIGAGGGFVELNDGMLYHKTVPASEVRRMVDFFDQHGVDFY
                     LESNGGLFASKNFLPHVERCIYGDIANDPDARRRKEEQPHPFIEGLIYGEEDLYKTDV
                     NKACFLQSDKLSFAQIKAEFENAFDTIQCTVPQFGDDSGELMVPGVHKATAIEALLTH
                     LSLPKERTIAIGDGLNDLEMFDYCAVGIAMGNAKEELKAVADHITTSVDDDGLYQAFR
                     TFGLV"
     gene            1321..>1986
                     /gene="proB"
                     /locus_tag="PLT73_08050"
     CDS             1321..>1986
                     /gene="proB"
                     /locus_tag="PLT73_08050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRM20363.1"
                     /translation="MEPIRNKTLDCKRIVIKIGTSSLMLADGSVNYQTIDRLAYVLTV
                     LRNQGKEIVLVSSGAIGVGLNHLNLPHRPKSIPEQQAVASVGQAELMNLYTRFFSHYN
                     QIVGQILMTLDIVQFPESRRNAVNAFEQLLKMGIIPIVNENDAVSVEELDHLTKFGDN
                     DRLSATVAEIISADLLIMLSDVPGFYDKNPTAHPDAVLFHTIHAVTSKEMALAGGNGS
                     KFGT"
BASE COUNT          535 a          461 c          464 g          526 t
ORIGIN      
        1 atcatcgcag ccgcatgctg tcaacaactt ttttaagttt cttataaaat tgcggttttc
       61 atgtcagcac acggttcctc tggctagcat tttcaggtgt tccattatat agtagaaatg
      121 aaaaacggag agccccatcg gagggcagaa agcgagaatt ataattggat aaaaaaatca
      181 tttttttgga tattgatggc actttggtga cggatgacgg ctgggttccc tcctccgctg
      241 cggacgcatg cagacaggca cgcttgaatg ggcacgaaat ttatttgtgc accggccgct
      301 caaaaccgga aatctatgat ttcatcatgg aaatcggctt tgacggcatc atcggtgctg
      361 gcggcggctt tgttgaactg aatgacggaa tgctttacca taagacagtc ccggccagcg
      421 aagtcagacg gatggtggat ttctttgatc agcatggcgt tgatttttac ttggaatcaa
      481 atggcggtct gttcgccagc aagaatttcc tgcctcatgt ggaacgttgc atttacggtg
      541 acatcgccaa tgatccggat gcccgtagac gtaaagaaga acagccgcat cctttcatcg
      601 agggattgat ctacggtgaa gaggatctgt ataagactga tgtcaataaa gcctgtttcc
      661 ttcagagcga caaactttct ttcgcacaga tcaaggcgga attcgaaaac gcctttgaca
      721 cgattcagtg caccgtccct cagtttgggg atgacagcgg tgaactgatg gtaccaggcg
      781 tacacaaagc gaccgcaatt gaagcgttac tgacacatct gagccttccc aaagagcgaa
      841 cgattgcaat cggcgatggc ttgaacgatt tggagatgtt cgactattgt gcggtcggga
      901 tcgccatggg gaatgccaag gaagaactga aggctgtcgc ggatcatatc acaacttcag
      961 tggacgatga tggtctctat caggcattcc gtactttcgg actggtctga ctgcgtcagc
     1021 ataaaagctt atccatttca taaaataatc ttcacagttt cttcataatt gagttttact
     1081 atgagtgtag gccaatcaca aacaatcttt ttcaatttct ttactcctcc aaagtaaaga
     1141 gcaactcctt tagggctgcc tgataacttc aggcagcttt tttttgctat atcttaattt
     1201 tttcgtcatt ccatgatact atatgaagta atacctcgct ggatgcgagc tctgcaacac
     1261 ataaaaaaac tattctttct atagaggaaa aaaaatcaaa tcgtaaacgg ggatgtgcat
     1321 atggaaccta ttcgcaacaa aacattggac tgcaaaagga tcgtcatcaa aatcggcaca
     1381 agttctttga tgctggcgga cggatccgta aactaccaga ctatcgaccg acttgcttat
     1441 gtcttgaccg ttctgcgcaa tcaggggaag gaaatcgttt tggtatcttc cggtgcgatc
     1501 ggtgtcggac tcaaccacct gaacctaccg caccgtccga aatccatacc cgagcaacag
     1561 gcagtcgctt ccgtcggtca ggccgaattg atgaacctct acacccgctt tttctcgcac
     1621 tataaccaga tcgtcgggca aatactcatg actttggata tcgttcagtt cccggaaagc
     1681 cgcaggaatg ccgtaaacgc cttcgaacag ttgttgaaaa tgggcatcat cccgatcgta
     1741 aacgagaatg atgctgtttc tgtcgaagaa ctggatcacc tgacaaaatt cggagataac
     1801 gaccgccttt ccgcaacggt tgcggagata atctccgccg atctgttgat catgctgtcc
     1861 gatgtacctg gtttttacga taaaaatcca acggcgcatc cagatgctgt gctcttccac
     1921 accatccatg cagtcacttc aaaggaaatg gctttggctg gcgggaacgg ttcgaaattc
     1981 gggacc
//
LOCUS       DAOMUJ010000350         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16161_length_1986_cov_2.204039, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000350 DAOMUJ010000000
VERSION     DAOMUJ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16161_length_1986_cov_2.204039"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLT73_08055"
     CDS             complement(<1..191)
                     /locus_tag="PLT73_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRM20364.1"
                     /translation="MAFEGLSERLQGAMTKIGKKGKITEADLKEMMREVRLALLEADV
                     NFKVVKDFVKKVNERALGS"
     gene            complement(212..559)
                     /locus_tag="PLT73_08060"
     CDS             complement(212..559)
                     /locus_tag="PLT73_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HRM20365.1"
                     /translation="MELEKTNNMNTLYEFYNVLLTNKQKGYLSLYYGDDYSLGEIAEE
                     FEVSRQAVYDNIKRTEKILMDYEQKLKLAQNYRLRNKKLDELANYAEEKYPDDRSLQR
                     LIANLEELDDKDE"
     gene            complement(782..1795)
                     /gene="ftsY"
                     /locus_tag="PLT73_08065"
     CDS             complement(782..1795)
                     /gene="ftsY"
                     /locus_tag="PLT73_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006418496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRM20366.1"
                     /translation="MGLFDRIKRAFTGEDVVQTPVTEENKIVIDKFDKGMEKTRKSFS
                     EKMNELFAGFREVDEEFFDDLEETLISADVGFDMTLALSDVLRDEVKMKNVRTGEQVK
                     NVIIEKMVEIYEKGQTELPTIKINENGPTVILFVGVNGVGKTTTIGKYAYQLKNQGNS
                     VLLAAGDTFRAGAIEQLEVWGQRVGVPVVTSDAKSDPASVVYDALKQAKEENYDYLLI
                     DTAGRLQNKVNLMKELEKINRIIAREIPGGATETLLVLDATTGQNALIQAKQFKETTD
                     VTGLILTKLDGTAKGGVILAIRQEMDIPVKFVGLGEGLDDLQPFDPEQYIYGLVKDLL
                     EKK"
     gene            complement(1828..>1986)
                     /locus_tag="PLT73_08070"
     CDS             complement(1828..>1986)
                     /locus_tag="PLT73_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM20367.1"
                     /translation="GDEENDLAMLDFAGVSVAMGNAPDKIKERASFITKTNGEDGVAH
                     AIETFVFA"
BASE COUNT          452 a          544 c          362 g          628 t
ORIGIN      
        1 tcagagccca acgctcgctc attcactttt ttgacgaaat cttttactac tttaaaattc
       61 acgtcggctt ccagtaaagc cagacgtact tcacgcatca tttccttaag atcggcttcc
      121 gtaatcttgc cttttttgcc gattttggtc atcgcgcctt ggagacgctc ggacaatcct
      181 tcaaatgcca tattttccac ttccttactg tttattcatc tttgtcatcc agttcttcca
      241 gatttgcgat cagcctttgc aaggatctgt cgtcgggata tttttcttcg gcgtaattcg
      301 ccaattcatc caacttttta ttccggagtc tatagttttg cgccagcttc aacttctgtt
      361 cataatccat cagaatcttt tcggtgcgct tgatgttgtc gtacactgcc tgccggctga
      421 cctcgaactc ttcagcgatt tcgcccaacg aatagtcatc accataatac aaagaaagat
      481 agcccttctg tttgtttgtc aacaggacat tatagaattc ataaagagta ttcatattat
      541 tggttttttc cagttccatc agaatacctt ccttctcgta cggattgtca agaaaaaatc
      601 tttacacttc attagattac cgattttgcc aagcttagtc aaccataaat tgcactgaag
      661 cagtgtcacc acaacaaaaa gcaaaatcag gccggcaatc ccgatgcgat tgccggcctg
      721 accatgcttc tgtcggcact tactatgaaa cgtgccttgc gaggcaggac gatctagttc
      781 cttatttttt ctcaagcaga tcctttacca atccgtaaat atactgctcc ggatcaaagg
      841 gctgcagatc atccaggcct tcacccaacc cgacgaattt gacggggatg tccatttctt
      901 gacgaatcgc caggatgacc ccgcctttcg ccgttccgtc caatttcgtt agaatcagac
      961 cggtgacatc ggtcgtctcc ttgaattgct tcgcttgtat caaagcgttc tgaccggtcg
     1021 tggcatccaa caccagcaat gtttcggttg cgccgccggg tatttctctt gcgatgatcc
     1081 gattgatttt ctccaattcc ttcatcaggt tcactttgtt ctggagtctt cctgccgtat
     1141 cgatcaacag gtagtcataa ttttcttctt tggcttgctt cagggcgtca tatacgacgg
     1201 aagccggatc gctttttgca tcgcttgtca caaccggcac gcctacacgt tgcccccaaa
     1261 cctccagctg ttcgatagct ccggctcgga aagtatcccc ggccgccaac aagacgctgt
     1321 tcccttggtt cttcaactgg taagcatatt ttccgatggt agtcgttttc cccactccgt
     1381 ttacgcccac aaaaagaatg acggtcggcc cattttcatt tattttgatg gtaggcaatt
     1441 ccgtctggcc tttttcatag atttcgacca ttttttcgat gatgacattc ttcacttgtt
     1501 ctcccgtgcg cacgttcttc atcttgactt catcacgcag tacatccgat agtgccaacg
     1561 tcatatcaaa cccgacgtct gcggaaatca gcgtttcctc caaatcgtca aaaaactctt
     1621 catctacttc ccgaaaaccg gcgaatagtt cattcatctt ctctgaaaag cttttgcgtg
     1681 tcttttccat gcctttatcg aatttatcga tgacaatctt attttcttca gttaccggtg
     1741 tctgaacaac atcttcacct gtaaatgcgc gctttattcg atcgaataat cccatttgaa
     1801 cacttcctct cttatgccag tctgaaatta cgcaaagacg aacgtttcga tggcgtgtgc
     1861 aacgccatct tccccgtttg ttttcgtgat aaaactagcc cgttccttga ttttatctgg
     1921 tgcgttgccc atcgcgacgc ttacacccgc aaaatccagc atcgccaaat cgttctcttc
     1981 atctcc
//
LOCUS       DAOMUJ010000351         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16240_length_1978_cov_2.065003, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000351 DAOMUJ010000000
VERSION     DAOMUJ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16240_length_1978_cov_2.065003"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLT73_08075"
     CDS             complement(<1..358)
                     /locus_tag="PLT73_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM20368.1"
                     /translation="MKKGFMKMVLGTMSLLALAACGNTSGTANSAAEADGALQEIKDS
                     GKLVVGTCADYPPYEWHLIKDGKDEIIGFDIDIAQAIADELGVELEIKDMDFDGLIPA
                     LSTGKIDMIIAGMNPTD"
     gene            complement(441..1292)
                     /locus_tag="PLT73_08080"
     CDS             complement(441..1292)
                     /locus_tag="PLT73_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM20369.1"
                     /translation="MKKGLLKLALGAMSILALAACGNTSETSDSSAIDSAATTTTDKL
                     QEIKDSGKLVMGTSADYPPYEWHLIKDGKDEIIGFDIDIAQAIADELGVELEVKDMDF
                     DGLIPALTTGKIDIIIAGMNATEERKQSVDFTDVYYTQTDIVVIRKEDADKFTSEDSL
                     KTAKLATQKATVQETYLLEAFPDAEIQSVPKWNTAIMSLTTGKVDAVMMVDTVAKQFI
                     AQNDDLMVANFDINSTPNAAAIAVAKNGGEFLETVNNIVNEMKESGKIEELYQLNDQI
                     VTDNTAE"
     gene            complement(1321..>1978)
                     /locus_tag="PLT73_08085"
     CDS             complement(1321..>1978)
                     /locus_tag="PLT73_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM20370.1"
                     /translation="QINSGEVVVIIGPSGSGKSTFLRCMNLLEEPTSGKVIFEGQEIN
                     KKGVDIDSIRTKMGMVFQGFNLFPHLTVLDNIMIGPQQVKKVPKDKAEAIARKLLARM
                     GMSEKADVYPQSLSGGQKQRIAIARALAMEPDMMLFDEPTSALDPEMVGEVLQVMKDL
                     AMEGMTMVVVTHEMGFAKEVGDRILFMDDGQVMEQGTPDEIFNHPKSERTKDFLSKVL
                     "
BASE COUNT          418 a          504 c          403 g          653 t
ORIGIN      
        1 catcagtcgg gttcatgccg gcgatgatca tatcgatttt gcctgtcgac aacgccggaa
       61 tcaagccgtc gaaatccatg tctttgattt ccaactcaac gcccagttca tccgcaatgg
      121 cttgcgcgat atcgatgtca aagccgatga tttcatcttt cccatctttt atcaggtgcc
      181 attcgtatgg gggataatcc gcacaggttc cgacgaccaa ttttccgctg tctttgattt
      241 cctgcaatgc gccgtctgcc tcagcagcgc tgttcgctgt cccgctcgtg tttccgcatg
      301 ctgccaaagc caagaggctc atcgttccca aaaccatttt cataaaccct tttttcaatc
      361 ctgattcctc cgcattcatt gcctcaaaaa aaggatgccc ttttcagagc atccttgcac
      421 ttttattggt atgttaattt ttattctgct gtattgtctg ttacgatttg atcgttaagt
      481 tggtataact cttcgatttt gccgctttct ttcatttcat ttacgatatt gttgactgtt
      541 tccaggaatt ctccgccgtt tttcgcaact gcgatagctg ctgcatttgg cgtgctgttg
      601 atatcaaagt tggcaaccat caaatcatca ttttgtgcaa tgaattgttt cgcaacggta
      661 tccaccatca tgacggcatc cacttttcct gtcgtcaagg acatgattgc tgtgttccat
      721 ttcggtacgg attggatttc agcgtctggg aaagcttcca gtagatacgt ttcttggact
      781 gtagcttttt gcgtagccaa tttagctgtc ttcaagctgt cttctgaagt gaacttatca
      841 gcatcttctt ttctgatcac gacaatgtct gtttgcgtat agtagacatc cgtgaaatca
      901 acgctttgtt ttctttcttc agtggcgttc attccagcga tgattatatc gattttaccg
      961 gtcgtcaacg ctgggatcag tccgtcgaag tccatatctt tgacttccaa ctcaacgcct
     1021 aattcatcag cgattgcttg ggcgatgtcg atgtcgaatc cgatgatttc atcttttccg
     1081 tctttaatca ggtgccactc gtatggagga taatccgcag atgtacccat taccaatttg
     1141 ccgctgtcct tgatttcctg caatttatca gttgttgttg tagctgctga atctattgcg
     1201 gagctgtccg atgtctcgct tgtatttccg catgccgcta aagcaagaat gctcattgca
     1261 cccaaagcta atttcaataa tccttttttc atcctaaccc ctccaaatat gttttttatt
     1321 ttataggact ttcgataaga aatcctttgt ccgctcactt tttggatgat tgaaaatctc
     1381 atccggtgtt ccttgttcca tcacctgacc gtcatccatg aaaaggatgc gatcgcctac
     1441 ttctttcgca aagcccattt cgtgggtcac gactaccatc gtcatgcctt ccatcgcaag
     1501 gtctttcatt acctgcaata cttcgccgac catttccggg tcaagcgcgg atgtcggttc
     1561 atcgaacagc atcatgtccg gttccatcgc caaagcccgt gcgattgcga tcctttgttt
     1621 ctgtccgccc gacaaggatt gtggatagac atcggctttt tcggacatgc ccatgcgcgc
     1681 caacagtttt ctggcgatcg cttcggcttt atcttttggt actttcttga cttgttgcgg
     1741 tccgatcatg atgttatcca ataccgtcag atgcgggaaa agattgaagc cttggaaaac
     1801 catccccatt ttggtgcgga tggaatcgat gtccacacct tttttgttga tttcctggcc
     1861 ttcgaagatc actttcccgg atgtcggttc ttccaacaga ttcatgcaac gcagaaacgt
     1921 gcttttgccg gatccggacg ggccgatgat aacgaccact tcacccgagt tgatttgt
//
LOCUS       DAOMUJ010000352         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16306_length_1972_cov_1.773605, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000352 DAOMUJ010000000
VERSION     DAOMUJ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16306_length_1972_cov_1.773605"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..775)
                     /gene="tmk"
                     /locus_tag="PLT73_08090"
     CDS             complement(134..775)
                     /gene="tmk"
                     /locus_tag="PLT73_08090"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRM20371.1"
                     /translation="MRGIFITIEGPDGSGKTTALQQVVPRLQQEMNRKVVATREPGGS
                     PIAEKIRSLILDPSHTDMDSRTEALLYAASRRQHLIEKVLPVLESGDVIFCDRFVDSS
                     IAYQGYARGIGEEGIREINQFATEGIEPDVTLYIDVPAEVGIQRIHANLDEREYNRLD
                     QEKLDFHEKVRAGYQQLAKANPERIVVVDGTMSREEVAESCYQIIKNRYPSYF"
     gene            complement(1169..>1972)
                     /locus_tag="PLT73_08095"
     CDS             complement(1169..>1972)
                     /locus_tag="PLT73_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20372.1"
                     /translation="RIFEYFDLPVEIDNDPQALRPESFAGELAFEEVSFHYDPDQPIL
                     NNVSFELKPGSSVAIVGPSGAGKSTIINLIPRLYDVTGGRIRLDGVDIRKLDLAFLRQ
                     HIGVVTQDTYLFNGTIRENLLYAKPDATQAEIEQACSEANIHAFISGLPKGYDTVVGN
                     RGMKLSGGEKQRLSIARIILRKPGLIIFDEATSSLDSISEHAIQEAIEPILAKSTSLI
                     IAHRLSTILSADEILVLEKGKIVERGDHETLVKKGGIYTMLYNTQMKQT"
BASE COUNT          469 a          522 c          431 g          550 t
ORIGIN      
        1 ttgaatgata gccataatta atttcataac aaatcccctc tttctataat atatgaaaaa
       61 attttataaa ttcctatatg ctgagaaaag cggaacggtc cgttgagctt ttgtttcggc
      121 aaatgatttt actctaaaaa tagcttggat atctgttttt gatgatctga tagcaagatt
      181 ccgctacctc ttcacggctc atcgtcccat cgacgaccac tatccgttcc ggattagcct
      241 tcgccaattg ctgatagcca gctcggactt tttcatggaa gtcgagcttt tcctggtcca
      301 aacgattata ttcccgttcg tccagattcg catggatcct ttgaatgcct acttccgccg
      361 ggacatcgat gtacaacgtc acatccggct cgatgccttc tgttgcgaat tggttgattt
      421 cgcggatacc ctcttcaccg atgcctcttg cgtagccttg ataggcgatt gaactatcaa
      481 caaaacgatc acaaaaaatg acgtctccac tttccaaaac gggcaacacc ttttcgatca
      541 gatgctggcg gcggcttgcc gcatacaaca aagcttctgt cctagaatcc atgtccgtat
      601 gcgaaggatc cagtatcaac gagcggatct tttccgcgat cgggcttcca cccggttctc
      661 tcgtcgctac tactttacgg ttcatctctt gttgcagacg cggtactact tgctgcaacg
      721 cggttgtctt gcctgagcca tccggccctt cgattgtgat gaaaatccct ctcactttgc
      781 acactcctac gattctaata cttccattat acgctaaatg aaagtttttt tatacagatg
      841 aaaccgatat agctttgtca attttgcaac aattaattgt cttgattgcc tttttctgaa
      901 aatagagcca tgaaactgtt tccggctccg ggtgcgcctg gattacgtgt gccgacaagc
      961 tgaaaagata aatacccgtt gcttattcat gtttccgact acattccggc ctctaaacat
     1021 gaatacccga gatttattca tgttttcgac cacatttcga cctcaaaaga tgaattcttt
     1081 cgatttattc atgttttcgg cgaacatccg ccaatcccca caaaaaaagg gacaacccca
     1141 ttccacatgg gcgcccctct tgataatcct atgtttgctt catttgcgta ttgtacagca
     1201 tcgtgtagat gccgcccttt ttgacgagcg tctcgtggtc gcctcgttca acgattttgc
     1261 ctttctccag caccaagatt tcatcagccg acaggatcgt cgaaagacga tgcgcaataa
     1321 tcaggctcgt gctcttggct aggatcggct cgatcgcttc ctggatggcg tgctcagaga
     1381 tggagtccaa tgaggaagtc gcttcgtcga agatgatcag ccccggtttc cgaagaatga
     1441 tccgcgcaat cgaaagccgc tgcttttcgc cgccggaaag cttcatgcca cggttaccca
     1501 ctacggtgtc gtaacctttc ggcagcccac tgatgaacgc gtgaatattg gcttcactgc
     1561 acgcttgctc gatttcggcc tgcgtagcat cgggcttggc gtagagcaga ttttctctta
     1621 tcgtaccgtt gaacaaatac gtgtcctgcg tcaccacgcc gatgtgctgg cgtagaaagg
     1681 ccagatcgag tttgcgaata tcgacgccgt ccaatcggat tcttccgcct gtcacatcat
     1741 agaggcgcgg aatcaggttg atgatggtgg acttgcccgc tcccgaaggc ccgacgatgg
     1801 cgacggatga tcccggcttc agctcgaaac tgacgttgtt caatatcggc tggtccggat
     1861 catagtggaa ggaaacctct tcaaaagcca actcgccagc aaacgactca ggccggagcg
     1921 cctgcggatc attgtcgatt tccacaggca gatcgaaata ctcgaatatg cg
//
LOCUS       DAOMUJ010000353         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16307_length_1972_cov_1.695357, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000353 DAOMUJ010000000
VERSION     DAOMUJ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16307_length_1972_cov_1.695357"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="PLT73_08100"
     CDS             <1..112
                     /locus_tag="PLT73_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRM20373.1"
                     /translation="KILALTDQNLYIPIFGEAESLNVAVAAGILLYHFVG"
     gene            452..>609
                     /locus_tag="PLT73_08105"
     CDS             452..>609
                     /locus_tag="PLT73_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM20374.1"
                     /translation="MEVKKCICPKFEKTFSILGKKWTGLIIEVLMDGDKRFKELAAQI
                     PNVSDRVLV"
     assembly_gap    610..619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <620..841
                     /locus_tag="PLT73_08110"
     CDS             <620..841
                     /locus_tag="PLT73_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRM20375.1"
                     /translation="YIFRHLREMDYTRYACIGTRVSKAKLKELEDEKIVVRTENPAAA
                     IKVMYGLSEKGKALNSVMQEIQTWSDAWV"
     gene            1171..>1972
                     /gene="pheS"
                     /locus_tag="PLT73_08115"
     CDS             1171..>1972
                     /gene="pheS"
                     /locus_tag="PLT73_08115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM20376.1"
                     /translation="MELKEKLQTLRIDALQQVESAEELQSLNQVRISYLGKKGPITEA
                     LRGMKDLSPEERPVIGTLANELRDDIEAAIQAKKDALEIKKMEAEIAAETIDVTLPGK
                     KIAQGTTHVLTQINEEIEDLFLGMGYKIVDGPEVEEDSYNFEKMNLPKDHPARDMQDT
                     FYITDELLLRTHTSPVQARTMEKHDFSKGPLKMISPGKVYRRDSDDATHSHQFHQIEG
                     LVIGENITLADLKGTLAVFAKKLFGEEREIRLRPSYFPFTEPSVEVDVS"
BASE COUNT          603 a          375 c          474 g          510 t
ORIGIN      
        1 aaaaatattg gcgttgacgg atcagaattt gtacattccg atttttggcg aagccgagtc
       61 cttgaatgtt gcggtcgcgg caggtatctt gctttatcac ttcgtcggct gaagaaatct
      121 ttaaaaaatc gctatttttt catttaaacg tgaaaaagta ttgcctttgt gaaattccta
      181 tcctattata agaaacagac atgaactttt tgtacagctt gcaagctggt tgccgaatat
      241 tcatgaatat ttttcgtgaa gtttttccta atttgatgga aatattcggc gcctgcaggc
      301 atttatctgg ggaaccaatt cagttagtgc tgtttgcggg ataggatgct tattttttgt
      361 aaacattttg tgacaaaagg cacattccct ttgacagggg aaatgagggt gtttataata
      421 ggtcaaagag gtgaaggctg tgaatgaaat gctggaagta aaaaaatgta tttgtccaaa
      481 attcgaaaag acattctcta ttttagggaa gaaatggacc ggcttaatca tcgaagtctt
      541 gatggacgga gataaacgat tcaaggaatt ggccgcacag atacctaatg tcagtgatcg
      601 agtattagtn nnnnnnnnnt acatattcag acatctccgg gaaatggatt atacccgtta
      661 tgcatgtatc ggtacccgag tatccaaggc gaagttaaaa gagctggaag atgaaaaaat
      721 tgtggtaaga accgaaaatc ctgcggcggc aatcaaagtg atgtacggat tgtcggaaaa
      781 aggtaaggca ttgaacagtg tgatgcagga aatccaaact tggtcggatg cgtgggtctg
      841 aatccgaact tgacggaaac ggcatttccg gttaaactgg caaagtaagt aaaatgaata
      901 cgaaaagcgt tgaagggaac cttcacaatc tgcaactttt ttcagggaat aatggccatt
      961 gactgcgagc ctttatagaa gatgattgtg ttttttcact ccccgagctg actttgggat
     1021 ttgccctgtg caatgaataa agttccggtt tattaccgtt aaacaaatga agtgtcgttg
     1081 cttgcaacga aacaagggtg gtaccacgaa aatctcgtcc cttaccaggg acgggatttt
     1141 tttgcatttt ttatgagaag gagacagaac atggaattaa aagagaaatt acaaacgtta
     1201 cgtatagatg ccttgcagca agtcgaatct gcagaggagc tgcagtcatt gaatcaagtc
     1261 cgtatttctt atttgggaaa aaaaggacca atcacagagg ctttgagagg gatgaaagat
     1321 ctgagtcctg aagaacgccc ggtcatcgga actttggcaa atgaattgcg tgacgatatt
     1381 gaagccgcca tccaagccaa aaaagatgcg cttgaaatta aaaaaatgga agctgaaatt
     1441 gcggctgaaa cgatcgatgt caccttgccg ggcaaaaaaa tcgcccaagg cacgacacac
     1501 gtcctgacgc aaatcaatga agaaatcgaa gacctgttcc tcggaatggg atacaaaatc
     1561 gtcgacggac cggaagtcga agaagacagc tacaacttcg aaaagatgaa cttgccgaaa
     1621 gatcatccgg cccgcgatat gcaggacact ttctacatca ccgatgaatt gttgttgcgt
     1681 acgcatacgt cacctgtcca agcgcgcacg atggagaagc atgatttttc caaaggtccg
     1741 ttgaagatga tcagtcctgg gaaagtctat cgccgtgata gcgatgatgc gacccactcg
     1801 caccaattcc accagattga aggattggtc atcggagaga acatcacttt ggctgaccta
     1861 aaggggaccc ttgccgtctt cgccaaaaaa ttgttcgggg aagaacgtga aatccgttta
     1921 cgccctagct acttcccgtt cacggaacct tctgtggaag tcgatgtcag ct
//
LOCUS       DAOMUJ010000354         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16400_length_1964_cov_3.465165, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000354 DAOMUJ010000000
VERSION     DAOMUJ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16400_length_1964_cov_3.465165"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLT73_08120"
     CDS             <1..443
                     /locus_tag="PLT73_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM20377.1"
                     /translation="TDRRFLLFLPHIQLMKELDGWLRELFPDKQFTCVSASDPDREEK
                     VKKMRSEEYDFLMTTTILERGVTFRDIDVIVLGAEDRVFTEASLVQIAGRAGRHKDYP
                     TGWVCFAHYGETKAIQGAVRQIRMMNRDAGRRGLLNGTMSLLPK"
     gene            415..1107
                     /locus_tag="PLT73_08125"
     CDS             415..1107
                     /locus_tag="PLT73_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRM20378.1"
                     /translation="MGQCLFCQNEIREVLTLQELFGFGKKNRGRVCRSCEEKLPRLAG
                     QTVCPGCMRPQASSVDLCEDCRRWRSCYPELTIAHHACYAYTGIVKDWLQRYKFQGDY
                     RLAGAFTNDLQALQRTHPQALFLPLPISIASYEERGFNQCEELLKQAGIRCVQFLGNQ
                     HAGEKQSEKNRQERLLTMQPFLLLEGYEKYLQQEIILFDDVYTTGRTLYHAKILLYKH
                     GFRMVRSTTIAR"
     gene            1234..1791
                     /gene="raiA"
                     /locus_tag="PLT73_08130"
     CDS             1234..1791
                     /gene="raiA"
                     /locus_tag="PLT73_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRM20379.1"
                     /translation="MFKYNVRGENIEVTEPIRSYAEKKLGRLEKYFGNVPETTAHVNL
                     KVYPFPAEKSAKVEVTVPLPFLVLRAEETSSDLYGSIDLVVDKLERQVRKYKTKIHRK
                     SRERGFDIGNEPNLIEEKMEDDENPLEIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIF
                     EDAETNGTSIVYRRKDGKFGLIETD"
BASE COUNT          573 a          429 c          512 g          450 t
ORIGIN      
        1 aaacggacag gcgttttctg ctgtttttgc cgcacatcca gctgatgaaa gaactggacg
       61 gttggctgcg ggaacttttt ccggacaagc aattcacctg cgtttcggcg agcgatcctg
      121 accgcgagga aaaagtaaag aagatgcggt ccgaggaata tgattttttg atgacgacga
      181 ccatactgga gcgcggggtg accttcaggg atatcgatgt catcgtgctt ggagcggaag
      241 atcgggtctt tacggaagct tcgctggtgc agattgccgg cagggcaggc aggcacaaag
      301 attatccgac aggctgggtc tgttttgccc actacggcga aacgaaagca atccaggggg
      361 cggtccgtca aatccgcatg atgaacaggg acgcaggaag aagggggctg ctgaatggga
      421 caatgtctct tctgccaaaa tgagatccgg gaagtattga cgcttcaaga acttttcggt
      481 ttcgggaaaa agaatcgcgg aagggtctgt agatcctgcg aggaaaaatt gccgcgttta
      541 gccgggcaaa ccgtatgtcc gggctgtatg cggccgcaag catcatccgt ggacctgtgc
      601 gaggactgcc gaagatggcg cagctgctat ccggagctga cgattgcgca tcatgcctgc
      661 tatgcctata cgggcatcgt gaaggactgg ctgcaacgct acaagttcca gggcgattat
      721 cgtcttgccg gtgccttcac gaatgatctg caggccctcc aacgtacgca tccccaagcg
      781 ctattcctgc cgctgccgat ttcaatcgcc agttatgagg agaggggttt caaccaatgc
      841 gaggagctgc tgaaacaagc aggcatccgc tgcgtacaat tcttgggaaa ccaacatgcc
      901 ggtgaaaagc aatccgaaaa aaacagacag gaacggctat tgacgatgca accattttta
      961 ttgctggagg ggtacgaaaa atatctccag caggaaatta tcctttttga tgacgtctat
     1021 actaccggaa gaacgctcta ccacgcgaaa atactcctgt acaagcatgg gttcagaatg
     1081 gtccgttcaa ctacgattgc aagataataa atcataaacg ttgaaatgtt aaggcgctta
     1141 cattataatg aagttaaaga aaccccctat gagtggtcca taggcgaggg ttgtctttat
     1201 ctgacgatgc gtcagacgaa aggagagagt gctatgttta agtataatgt ccgaggagaa
     1261 aatattgagg ttacagaacc gatccgcagc tacgccgaga aaaaattagg cagactggaa
     1321 aaatactttg ggaacgtgcc tgaaacaaca gcgcatgtca acttgaaagt atatccattc
     1381 ccagccgaaa aatctgctaa ggtggaagtt acggttccgc tacctttcct ggttttaaga
     1441 gccgaagaga cttctagtga tctatacgga agtatcgatt tggtcgtgga caaacttgaa
     1501 cgccaagtcc gtaagtacaa aacaaagatt caccgcaaat ccagagagag aggatttgat
     1561 atcggtaacg agccgaatct gatcgaagaa aagatggaag acgatgagaa tccacttgaa
     1621 atcgtccgca cgaaacgtct ttccctcaaa ccgatggaca gtgaagaagc tgtattgcag
     1681 atgaatatgc tcggacacaa cttctttatc ttcgaagatg ctgaaacgaa cggcacaagt
     1741 attgtgtacc gcagaaaaga cggcaaattc ggtctgatcg aaactgacta aatctgaaaa
     1801 taatgtccct cgcccataaa cggcggggga cttttttaaa catataggaa tttataaaat
     1861 tttccagagt gaaaagtgca tcaccgtagg tgtttcacaa gtttgcaaaa tgtttcatgc
     1921 atctaaaatg atgtccagct tcacgggtta gcccttcgga aatt
//
LOCUS       DAOMUJ010000355         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16462_length_1960_cov_1.632021, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000355 DAOMUJ010000000
VERSION     DAOMUJ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16462_length_1960_cov_1.632021"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..1762
                     /locus_tag="PLT73_08135"
     CDS             566..1762
                     /locus_tag="PLT73_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010765560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM20380.1"
                     /translation="MPKIKIENVTKIFGKKADKALELLEQKKSKQDILRATGATVGVN
                     NVSLSIEEGEIFVIMGLSGSGKSTLVRMFNRLIEPTKGNILIDGENLSIMDKKALRQV
                     RREKMSMVFQNFGLFPHRTILENTEYGLEVQGVAKAERKERAQQALENSGLGDYSDQY
                     PHQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVSKTII
                     FITHDLNEALRIGDRIALLKDGELVQIGTPEEILTNPATRYVEKFVEDVDRSKVLTAE
                     HIMKRPIVMNIEKHGPLFALELLRQEGISTILVVDNHRRLKGYITAEDATKALKANAT
                     KVDGILREDIPTVSKDTTLSDIFPIIHDSNSPVAVVENDRLVGVLVRGAVIAALAGES
                     EVSVNG"
     gene            1755..>1960
                     /locus_tag="PLT73_08140"
     CDS             1755..>1960
                     /locus_tag="PLT73_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine ABC transporter"
                     /protein_id="HRM20381.1"
                     /translation="MDNLLNFIPELPVAEFTEYITDKTADTFSFLFDPIKESSQAAID
                     LLTSALTAVPAVILIALVALLAFFA"
BASE COUNT          561 a          436 c          491 g          472 t
ORIGIN      
        1 gctgctgacg actgaatggc gtgtacaagt taccggattt tcgcgaattg tcgaatgaac
       61 caccgctatt cgacaatctg cactctggaa acggcgaaat tgtcgaatag ctgatgacat
      121 tcgacaattt tgacattacg agccgccgta cagtcgaatg ccgggaacac attcgacaat
      181 ttgagctcta agcacagaaa aaacatcggt ggtgtccgta tgttgtgtgg tgcgtatgtc
      241 aacataacga ttcatccaag aagttaattt aaaaaaatcc ggatactccg cagttacctt
      301 taggaagcgt gtgtaaccgc atactttttc gctaaaaatt cacaaataat tcatttttga
      361 tttgcatatc cgcgccataa ctggataata gtggatatag ctttgtgatg taacaaaatt
      421 atcttcaatt tatgtttttg taataaatga aaatgtaaca ctatatttgt acattgcaga
      481 ggatcatagg cggatccggg atcacattcc cattaccaac agaatccacg cacatagcat
      541 caaaaatttg agaggagcaa tgcacttgcc gaaaataaaa atagagaacg taacgaaaat
      601 ttttggaaaa aaagcagata aggctctgga actgcttgaa caaaagaaga gcaaacagga
      661 catcctgcgt gccactgggg cgacagtagg ggtcaacaac gtcagtcttt ccatagaaga
      721 aggggaaatc ttcgtcatca tgggtctttc cggaagcggg aagtcgacac ttgtgcggat
      781 gttcaaccgt ctgatcgaac cgacgaaggg gaacatcctg atcgatggcg aaaatttgtc
      841 gatcatggac aagaaggcat tacgccaagt ccgtcgggaa aaaatgagta tggttttcca
      901 gaacttcggc ttattcccgc accgcacgat tttggagaac accgagtacg gactcgaagt
      961 gcagggcgta gcaaaagccg agcgcaagga aagagcgcaa caggctttgg aaaattcagg
     1021 cctgggcgat tacagcgatc agtatccgca ccaattatca ggtgggatgc agcaacgggt
     1081 cgggttggcc cgtgccttgg cgaatgaccc ggaaattcta ttgatggacg aggccttctc
     1141 ggctttggat ccattgatcc gtcgtgagat gcaggatgaa ctgttggatc tgcaggcaaa
     1201 tgtcagcaag accatcatct tcatcaccca tgatctgaac gaagctttgc gtatcgggga
     1261 tcggatcgcg ctgctgaagg atggggaatt ggtccaaatc ggtacgcctg aggaaatttt
     1321 gaccaatccg gcaacgcgct atgtcgaaaa attcgtggag gacgtcgacc gttcgaaagt
     1381 gctgactgcc gaacacatca tgaagcgtcc gatcgtcatg aatatcgaaa aacacgggcc
     1441 gctcttcgcg ttggaattat tgcgccaaga agggatttcg acgatcctgg tggtggacaa
     1501 tcaccgcaga ctgaaagggt acatcactgc cgaagacgcc acgaaagcgt tgaaagccaa
     1561 tgctacgaaa gtggatggca tcctcagaga agatatcccg acagtctcca aagatactac
     1621 cttgagcgat attttcccga tcatccatga ttcaaactca cctgtcgcgg tcgtcgaaaa
     1681 cgacagattg gtgggtgttc ttgtcagagg cgccgtcata gccgctttgg caggagaaag
     1741 tgaggtgtcc gtaaatggat aatctattaa atttcattcc tgaattgccg gttgccgagt
     1801 tcactgaata catcacggac aagaccgctg acacgttcag cttcctgttc gatccgatca
     1861 aagagtcttc gcaagctgca atcgatttgt tgacgtcggc actgacagcc gttccggcgg
     1921 tcatcctgat cgcattagtt gccttgttgg cattctttgc
//
LOCUS       DAOMUJ010000356         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16480_length_1958_cov_1.691014, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000356 DAOMUJ010000000
VERSION     DAOMUJ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16480_length_1958_cov_1.691014"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..362
                     /gene="gpsB"
                     /locus_tag="PLT73_08145"
     CDS             21..362
                     /gene="gpsB"
                     /locus_tag="PLT73_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HRM20382.1"
                     /translation="MADKNLTTKDILQKEFKSAMRGYNVAEVDEYLDMIIRDYESYQK
                     DLSYLQSENERLMSRVDELTKQAVLAKPTQSNVSSVGSGVTNFDILKRLSNLEKHVFG
                     SKLDETNEEAN"
     gene            381..746
                     /gene="rnpB"
                     /locus_tag="PLT73_08150"
     ncRNA           381..746
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLT73_08150"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            869..>1958
                     /locus_tag="PLT73_08155"
     CDS             869..>1958
                     /locus_tag="PLT73_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRM20383.1"
                     /translation="MKTYQLMATAASGIEAITGNELKKMGYNVQVENGKAYFTGTEAD
                     IITTNLWLRTADRIKIVFGKFEAKTFDDLFEQTKALPWEQILPMDAEFPVSGKSQKST
                     LYSVPDCQAIVKKAIVNRLSEYYHRRTRLPESGALYPIEVAINKDEVILTIDTSGSSL
                     FKRGYRSEKGGAPLKENMAAALVMLTNWFPDRPFYDPTCGSGTIPIEAALIGLNIAPG
                     LKRSFPSEEWNIFEEGMWDRIRNEAKAAANFDVKLDIGGSDIDPHVIAIAKSNAEKAG
                     VADYVTFKQLALADFTTTKEYGTIVCNPPYGERLSDQEEIEILYKEMGQVFRPLKTWS
                     KYILTSELLFEEFYGERATKKRKLYNGRL"
BASE COUNT          594 a          439 c          470 g          455 t
ORIGIN      
        1 aactaacgag gtgtgcaaat atggcagata aaaatctaac aactaaagat attctgcaaa
       61 aagaattcaa aagcgctatg agaggctata atgttgcaga agtcgatgaa tatctggata
      121 tgatcataag agattatgaa tcttatcaaa aagatctttc ttatcttcaa agcgaaaatg
      181 aacgtttgat gagccgtgtg gatgagttga cgaagcaagc tgttttagct aagccgaccc
      241 aatcgaatgt atcatcagtc ggcagcggtg taacgaattt tgatattctg aaacgccttt
      301 ccaacttgga gaaacatgta ttcggttcaa aattggatga aaccaacgaa gaagcaaact
      361 agcatcaata gacatttgta aatttcgggt aattgcggtc tccttcatgg aggctgagga
      421 aagtccatgc tcgcacaagc tgagatgctt gtagtgttcg tgcttagcga aacaataagc
      481 taaggcaatc gagagattga cggcagaaaa aaaggctaag ggctctgcct atgccggagt
      541 attcttgaaa gtgccacagt gacgaagcag acggggaaac ctttctggtg gaacgcggta
      601 aacccctcga gcgagcaacc caaactttgg taggggcact ctttccaagg aaatgaacga
      661 gggaaagagg cagaaatgca gatagataat tacctccgcc atcagaggcc tggctccgat
      721 ggcgtgacag aacatggctt acagaaattt acaaatcagg ctttttttct tcaaaaagaa
      781 tacgatcgag gatgggacaa gctaattttt gtcccatttt tcgtgcatta tccggcaatt
      841 tctaaggcaa aacaataagg agcactaaat gaaaacttat caactaatgg ctacggccgc
      901 aagcggcatc gaagcaatca ccggcaatga gctgaaaaaa atgggctaca atgtccaagt
      961 agagaacggc aaggcctact tcaccggtac ggaagcggac atcatcacca ccaacctgtg
     1021 gctgcgcacc gctgaccgca tcaaaatcgt tttcggcaaa ttcgaagcaa aaaccttcga
     1081 tgatcttttc gagcaaacga aagctttgcc gtgggaacaa atcctaccga tggatgcaga
     1141 attccctgtt tccggcaaat cacaaaaatc gaccttgtac agtgtccccg attgccaagc
     1201 gatcgtaaag aaggccatcg tcaaccgtct gagcgaatac tatcaccgtc gtacccgttt
     1261 gcctgaatcc ggcgcacttt atccgattga agtggccatc aacaaggatg aagtgatcct
     1321 gacgattgac acaagcggtt ccagtctttt caaacgcggt taccgttccg aaaaaggtgg
     1381 cgcaccattg aaggaaaata tggcggccgc tttggtgatg ttgaccaatt ggttccctga
     1441 tcgccccttc tatgatccga cttgcggttc cggcacgatt ccgattgaag cagctcttat
     1501 cggcctgaac atcgcgcctg gtttgaagcg ctcattccct tccgaagagt ggaacatttt
     1561 tgaagaaggt atgtgggatc gtatccgcaa tgaagccaaa gctgccgcga atttcgatgt
     1621 gaagttggat atcggcggtt cggacatcga tccacatgtc atcgccatcg cgaagagcaa
     1681 tgccgagaaa gcaggcgttg cggattatgt cactttcaaa cagttggctt tggccgactt
     1741 cacgactacc aaggaatacg gcacaattgt ctgcaacccg ccctacggtg agcgtctttc
     1801 cgatcaggaa gagatcgaga tactctacaa agagatgggc caagttttcc gcccattgaa
     1861 gacatggagc aagtacattc tgacgagcga attgcttttc gaagaatttt acggcgaaag
     1921 agctacgaaa aaacgcaaac tctacaacgg cagattgc
//
LOCUS       DAOMUJ010000357         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16591_length_1947_cov_2.099366, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000357 DAOMUJ010000000
VERSION     DAOMUJ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16591_length_1947_cov_2.099366"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLT73_08160"
     CDS             complement(<1..1254)
                     /locus_tag="PLT73_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20384.1"
                     /translation="MKNWKSVAIAYGLPAVVLLGYWYSALTPVSIQSPEFWTNMFLYL
                     VASAALMNVLDRSKADKVNKISGAIIGISVVFIIGGMLFGSQIFRAKDYASLITHTDG
                     NFEEDLTITDPSQIPTIDRDMAERLGSRKLGEVLNLVSQFDVSTEYTQIAYKGDSVRV
                     SPLEYVSFFRWLNNRADGIPHYVTVDMVTGDSDLVDLEKTIHYSHSGYFGDDITRHIF
                     FNYPTVMFENPVFELDEEGNPYYIAPIVEKKFSFMGPKEVVGVFVVDASSGDITRYSV
                     ADVPDWVDRVFPANMVMTQINYNGQYQGGFLNSVISKKGVIQNANGYNYIVLNDDLYL
                     YTGLTSVAADESNIGFVLVNSRTKESKRYNISTATEWSAMESAQGSVQEKGYISTFPL
                     LFNMENKPVYQLSLKDDAGLIKMYAF"
     gene            complement(1668..>1947)
                     /locus_tag="PLT73_08165"
     CDS             complement(1668..>1947)
                     /locus_tag="PLT73_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20385.1"
                     /translation="VEGTEKVWKADLVLLAMGFTGPEDTIPDELTLKRDLRSNIQAAE
                     TDYKTNVEKVFTCGDMRRGQSLVVWALQEGKLAAGEVDRFLSGDTAIY"
BASE COUNT          470 a          517 c          440 g          520 t
ORIGIN      
        1 aaaggcgtac attttgatca agccagcatc atctttcaag gataactgat agactggctt
       61 gttttccata ttgaacaaaa gcgggaatgt gctgatgtag cctttttcct gcacggatcc
      121 ctgggcgctc tccatggcac tccattcggt cgctgtgctg atgttgtagc gtttcgattc
      181 cttggtcctg gaattgacca gcacaaaacc gatatttgat tcatccgccg ccacggaagt
      241 caaccccgtg tacaggtaca gatcgtcatt gagcacgatg tagttgtagc cattcgcatt
      301 ttggatgaca cccttcttgc tgatgacgct gttcaggaaa ccgccttggt attgcccgtt
      361 gtaattgatc tgcgtcatca ccatattcgc cgggaacacg cgatcgaccc aatccggcac
      421 atctgcgacg ctgtagcgcg tgatgtcacc agaggaagca tccacgacga acacgccgac
      481 gacttctttg ggccccatga agctgaattt cttttcaacg atgggcgcga tgtaatacgg
      541 gttgccctct tcatccaatt caaagaccgg gttttcgaac atcacggtcg gatagttgaa
      601 aaagatgtgc cttgtgatgt catccccgaa atagccggaa tgggaataat ggatggtctt
      661 ttccagatcc accaaatcag aatctcccgt caccatatcg acggtcacat aatgtggaat
      721 gccgtctgcg cggttgttca accaacggaa aaaactgaca tattccaatg gcgaaacacg
      781 gaccgaatcg cctttataag cgatctgtgt gtattccgtg ctgacgtcga attgcgaaac
      841 caagttgagg acttcgccaa gcttacggct tcccagacgc tccgccatat cgcggtcgat
      901 cgtcgggatc tgcgaaggat ccgtgatggt caggtcctcc tcaaaattgc catccgtatg
      961 cgtaatcagg ctggcgtaat ctttggccct gaaaatttgg gaaccaaaaa gcatcccgcc
     1021 aatgatgaaa acaacgctga ttccgatgat ggctccagaa attttattga ctttatcggc
     1081 tttcgaccga tccaagacgt tcatcaatgc tgcacttgcg accaaataca aaaacatatt
     1141 cgtccaaaac tccggcgact ggatcgacac cggggtcaac gccgaatacc aatatcctag
     1201 aagaacgact gcgggtaggc cataggcaat cgccacactt ttccaatttt tcacaacttg
     1261 acaccctttc ttttcctgca aatttttccc tcttagatta cttaaggatt tgttgttatt
     1321 ataccatttt tttgagttat atttcggaat tcaatatatg gaattatgcg ggccgcccca
     1381 acagttttag gctcctgttc gagtttgcgt tcgaatgtgg gttcaccgga ggacagcact
     1441 ccgggaaggc gtcaactcct gcaagcgttg gctggccgga gttgacggca ttattcttgg
     1501 tctttctccg gcgaaggcgg gctcgccgga gttggcaact acttttcttt tttcatctcc
     1561 gggtacagca ccctcgccgg agcaaggcac acaactctcc cgctgtacca aatccgcaaa
     1621 aaaaccgaag caacgattcc ccgttgcttc ggtttttcca actaatatca atagatggct
     1681 gtgtcgcctg acaagaaacg gtccacttcg cctgcggcta gtttcccttc ttgcaaagcc
     1741 cagacaacta agctttgtcc tcggcgcatg tcaccacatg taaagacttt ttccacgttc
     1801 gttttgtagt cggtctctgc tgcttggata ttgctgcgca gatcgcgttt caaagtcaac
     1861 tcatccggaa tcgtatcttc aggaccggtg aaacccatcg ccaacaagac taagtccgct
     1921 ttccagactt tttccgttcc ttcgacg
//
LOCUS       DAOMUJ010000358         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16748_length_1934_cov_1.287387, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000358 DAOMUJ010000000
VERSION     DAOMUJ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16748_length_1934_cov_1.287387"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLT73_08170"
     CDS             complement(<1..627)
                     /locus_tag="PLT73_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM20386.1"
                     /translation="MKQSIPITVLTGYLGSGKTTLLNHILHGDHGLKIAVIVNDMGSV
                     NVDEALINHGGFKRTEEKLISMANGCICCTLREDLIVALDSLAQLPDIDYIVIEASGI
                     SEPIPIAQSLTLPENPLGIDLTGQVHLDTMVTVVDAKRIWDDFGSGQPIVDRQKEIVD
                     EENRDIRDLLIDQIEFCNVLILNKCDLLEEWQVEKIETLVHSLQREAKI"
     gene            981..1250
                     /gene="rpsN"
                     /locus_tag="PLT73_08175"
     CDS             981..1250
                     /gene="rpsN"
                     /locus_tag="PLT73_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRM20387.1"
                     /translation="MAKQSKIAKARKQREVIEKYAAVRKELKEAGDYQALAKLPKDSN
                     PNRYKNRDLIDGRPRAYMRKFGMSRISFRQLAHLGLIPGVQKASW"
     gene            1326..1475
                     /gene="rpmG"
                     /locus_tag="PLT73_08180"
     CDS             1326..1475
                     /gene="rpmG"
                     /locus_tag="PLT73_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM20388.1"
                     /translation="MRLNIILECVETGERLYLTSKNKRNNPERLEMKKYSQKLRRRAI
                     FREVK"
     gene            1505..1636
                     /locus_tag="PLT73_08185"
     CDS             1505..1636
                     /locus_tag="PLT73_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM20389.1"
                     /translation="MSISITVISGFLGAGKTSLINHVLAGTKLAAEEKGVRACLDRA"
     assembly_gap    1686..1699
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1712..1819
                     /locus_tag="PLT73_08190"
     CDS             1712..1819
                     /locus_tag="PLT73_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HRM20390.1"
                     /translation="MEKTDASSSYRRLKSTNIKTRKRALKIVKEAKRRR"
BASE COUNT          606 a          400 c          438 g          476 t
ORIGIN      
        1 gattttagct tccctttgaa gcgaatggac caatgtctcg attttttcga cttgccattc
       61 ctccagcaaa tcgcacttgt tcagaatcag cacattgcaa aattcaattt ggtcaatcaa
      121 cagatcgcgg atgtcgcgat tttcctcatc cacgatttcc ttttgccgat caacaatcgg
      181 ctgacctgag ccaaagtcat cccaaattct tttcgcatcc acaacggtca ccatcgtgtc
      241 caggtggacc tgaccggtca gatcaatccc caaaggattt tctggcagcg tcagcgattg
      301 ggcaatcggg atgggttcac tgattcctga cgcctcaatc acaatgtagt caatatccgg
      361 gagttgcgca agggaatcca gcgcaacgat taaatcttct ctcaatgtac agcaaatgca
      421 gccattggcc atcgaaatca gcttttcctc cgtccgtttg aatccgccat gattgatcag
      481 cgcctcatct acattgacac tgcccatatc gttgacgatg accgcaattt tcaagccgtg
      541 atcgccatgg agaatgtgat tcaataaagt ggtttttcct gagcctagat aaccggttaa
      601 taccgtaatg ggaatcgatt gtttcatcta aaatattctt cctctcttaa gacgcctgta
      661 ttcttggtta gttttgaaaa gcaaacactt aattccgtgc gtactcctgt tgttgatgcc
      721 tcaatagttg caaaacgtaa taattacgat ttaaagcgta tcggtaattt tttagattgt
      781 caagcttttt tagagtcgga ttgtaattga ggattgagca tcctgtcact atgctgtcgt
      841 gaaacgcact catatgaatg aaacttcaga tttatttatg ttgacaatcc atttatagta
      901 tgataaattt tcggttataa atcgtaaacg ttacgatttg aatcgaggcg gattgcatgg
      961 agcacagaaa ggaagagagt atggcaaaac aatcaaaaat cgcaaaagcc cgcaaacagc
     1021 gggaggtaat agaaaaatac gcagcagtgc gcaaggaact aaaagaggct ggcgattacc
     1081 aagcactggc aaaattgccg aaagattcaa atccgaaccg gtacaaaaat cgcgatttga
     1141 tcgatggcag accgagagcc tacatgcgca aattcggtat gtcacggatc agcttcagac
     1201 aacttgctca tctgggcttg attcctggtg tccaaaaagc cagctggtaa gaatgaacca
     1261 ataaaagagt acggatcaga aggaacagcc gatccggcaa aaaaattcag aaaggaatga
     1321 aaaaaatgag acttaatatt attttggaat gcgttgaaac aggggaacgc ctgtatttga
     1381 ccagcaaaaa caaacgcaat aatccagagc gcctggaaat gaaaaagtat tcccaaaagt
     1441 tacgcagacg cgcaattttc cgtgaagtga agtaagttaa aattcaagca atgggggaat
     1501 cgctatgagt atttcgatta cggtgatctc gggtttttta ggagccggga aaactagtct
     1561 gatcaatcat gtgctggcgg ggacaaagct ggcagcggaa gagaaggggg tcagagcttg
     1621 tcttgatcgg gcgtgaattg aatccggtaa tgatccgggc tgctttttag atagtccaag
     1681 atgcgnnnnn nnnnnnnnna ggagaatttc catggaaaaa acagatgcat caagttctta
     1741 ccgtcgtttg aaaagtacaa atatcaagac ccggaagcgc gctttgaaaa tagtcaaaga
     1801 agctaagcga agacgctgaa cacagaggaa aaaatgtcga acagccgacg aaaaacgagg
     1861 tcatcggctg aaaggatagc gaaaaacacc caacagccga cgaaagcagg aatcgtcgac
     1921 tgaacgaaga atga
//
LOCUS       DAOMUJ010000359         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16759_length_1933_cov_1.407881, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000359 DAOMUJ010000000
VERSION     DAOMUJ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16759_length_1933_cov_1.407881"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLT73_08195"
     CDS             complement(<1..854)
                     /locus_tag="PLT73_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM20391.1"
                     /translation="MTKLIIDSTCDLNEAMRKHPKLEYLPLNIIIDEKSYKDGIDIDL
                     ERVYEVMRKGEMPQTSQIDIASLLEILDRNRQAKEDLIYLSFSSAMSGTHAVAHQVFG
                     MYREEYPEMKLTLIDSQGGCGGASLAAIQALNLLDKGYSHDEVARTIQEIVAHTSYSF
                     TIANLEWLQKGGRINKVASIIGDNLNVKPLLTVKAGKIVMDQPLRGHKRVYRIIKKTV
                     DHIGPYQEQLIVISHVGDPEIAQTLKEMLSAALPQASFVLLRVSAVLGVHLGIGGVGV
                     FALSHLPE"
     gene            1211..>1933
                     /locus_tag="PLT73_08200"
     CDS             1211..>1933
                     /locus_tag="PLT73_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016647623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM20392.1"
                     /translation="MEEKVHTGLTYGVEDKPDLGTTIVLGFQNVITAFGGLVAVPLII
                     AGIAGFGVEDTAYMVSAALLASGIVSIIQSKGFGPKWFRVGAGLPTIMGTDFAFVAPA
                     ASVIGAGGITAYFGGTMLGALLEVGLSYFVKPLLKFFPPVVTGSVISLMGMTLMSVAM
                     GWAGGGFGSEDFGSPMNIGIAVLVFLAIALINHYGPSRIAPAAVLIGAAIGYVVCIPL
                     GLVDFGQVVAAEWFAFPQFFKFG"
BASE COUNT          454 a          416 c          473 g          590 t
ORIGIN      
        1 ctttccggca agtgggataa ggcgaagacg ccgactccac cgataccgag gtggacgcca
       61 agaaccgcgc ttacccttaa taggacaaag gaagcttgcg gcaaggctgc gctcagcatt
      121 tctttgagtg tttgtgcgat ttctggatcg cccacatggc tgatgacgat cagttgctct
      181 tgatagggac cgatatggtc gactgttttt ttgatgatac ggtagacacg cttatgtcca
      241 cgtagtggtt ggtccatcac gattttacct gccttaacag tcaacaacgg cttgacgttc
      301 agattgtcac cgatgatgct cgctacttta ttgatgcggc cacctttctg gagccattcc
      361 agattggcga ttgtgaaaga ataggaagta tgcgcaacga tttcttggat ggttcgggca
      421 acttcatcat gggaatagcc tttatccaac aagttcagtg cttggatggc agccaagcta
      481 gcaccaccgc aaccgccttg cgagtcaatc aaagtcagtt tcatttctgg gtattcttcg
      541 cgatacatcc caaatacttg atgggccact gcatgtgttc ccgacatcgc gctcgagaaa
      601 gacaaataaa tcagatcttc tttggcttgg cggttgcggt ccaatatttc caataggctg
      661 gcgatgtcaa tttgtgatgt ctggggcatc tctcccttgc gcatcacttc gtagacgcgt
      721 tccaagtcga tgtcgatgcc atctttgtag cttttctcat cgatgatgat gttcagaggc
      781 aggtactcca gtttgggatg tttgcgcatc gcttcgttaa ggtcacatgt tgaatcgatt
      841 attaattttg tcaaaatttc actccttatt tttcggcaat aactgcctgg tttggttcga
      901 ggtacgcccg atacacgttt cgcttcatac agtataacaa aaagtaaggc tacggcgcac
      961 gcatatctga caatgaccta tcaaccgtaa ttcgttatat tcaatacaaa taccaaaaaa
     1021 aaaatgtgga atgttcgctt ttatcccatt tcatgttaaa aaatgtgaat attctcataa
     1081 tgaaagccct tataattcgg tatttacact ttacaattgt atgttaactc gatataattt
     1141 tatttgtcat ttaataaacg aattataatt tgttttgaaa tcattaatgt tcgttatcag
     1201 gaggataaac atggaagaga aagtacatac aggcctgact tacggggttg aggacaaacc
     1261 cgatttaggc acgacaatcg ttttaggatt tcagaacgtc atcaccgctt ttggtggttt
     1321 agtggcagta ccgcttatca ttgccggaat cgccggcttc ggggttgagg atacggctta
     1381 tatggtcagc gcagcgcttt tggcttcggg tatcgtttcc atcatccaat caaaaggttt
     1441 tggtccgaaa tggttccgcg tcggagcggg cttaccgaca atcatgggaa ctgactttgc
     1501 ctttgtggca ccggctgctt ccgtcatcgg agcgggcggt atcactgcct atttcggtgg
     1561 tacgatgttg ggtgctttat tggaagttgg tttgagttat ttcgtaaaac cgttattgaa
     1621 atttttcccg ccggttgtaa ctggatcggt tatctccttg atggggatga cgctgatgtc
     1681 cgttgcgatg ggttgggcag gtggcggttt cgggtctgaa gatttcggta gtccaatgaa
     1741 tatcggtatc gcagtgcttg tgttccttgc catcgctttg atcaatcatt acggtccaag
     1801 cagaatcgcg ccggccgctg ttctgatcgg tgccgcaatc ggttatgtag tatgtattcc
     1861 gctagggctg gtagattttg ggcaagtcgt tgccgctgaa tggttcgcgt ttccacaatt
     1921 cttcaaattc gga
//
LOCUS       DAOMUJ010000360         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16785_length_1930_cov_1.907200, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000360 DAOMUJ010000000
VERSION     DAOMUJ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16785_length_1930_cov_1.907200"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1482)
                     /locus_tag="PLT73_08205"
     CDS             complement(244..1482)
                     /locus_tag="PLT73_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM20393.1"
                     /translation="MNTSRKAFRRLFGVILSLLLLFTLSATPVLAATSQIIYQGSTSN
                     KVVALTFDDGSDGYNIQSILNTLSANNIKATFFLTGQGAENHPQAIKNIVSAGHDIGN
                     HSYNHPDFTTVATTEMTNQLTRTETIITNLTGKSTKPFFRAPFGSYNSTVLQTVGNAG
                     YTYTFQWTIDSLDWTGNSATDIYNRVINNIVPGAIVLMHTGYGANGTTAALPNIINQL
                     RAMGYSFVTLSQLMNTTSTPGTGTGTSYTVKAGDTLYAIALRYGVTVQTIINANGITN
                     ANLIRVGQVLTIPGTSTPTTPTPPTTPTTGTTYTVKSGDTLYAIALRYGVTVQQIVTA
                     NNITNANLISIGQVLTIPGTGTPTTPTTPTTGTSYTVKAGDTLYAIALRYGVTITQLV
                     NANGIANPNLIRVGQVLIIP"
BASE COUNT          502 a          372 c          516 g          540 t
ORIGIN      
        1 ccagatcaac ggcaaagtaa aaaccaaaga aatggttccg tccgatattt caaatgatga
       61 catgatcgcg atggcattgg aaaacagcat catcaaagaa ggcatcgaag gtaaaacggt
      121 aaggaaaaca atcgccatcc caggcaaatt ggtcaatatt gtagcgaatt aatcttaaca
      181 tatcaaaaag aacgcacacc ttcagctttg gggtgtgcgt tctctttttt ttctgtgtta
      241 tgcttaaggg atgatcagca cctgaccgac ccggataagg ttcggattgg ctatgccgtt
      301 ggcgttaacg agctgggtga tggttacacc gtagcgaagc gcaattgcat ataaagtgtc
      361 tccggctttg accgtataag acgtgccggt tgtaggcgta gttggagtcg tcggcgttcc
      421 tgtgccaggt atcgtcaata cttgaccgat actgatcaga ttggcgttgg tgatgttatt
      481 agcggtaacg atctgttgga ccgtcacccc ataccgcaag gcgatggcat agagtgtatc
      541 cccggatttg actgtgtaag ttgtgccagt cgtcggcgtt gtagggggcg taggtgttgt
      601 cggcgtgctt gttcccggaa tcgtcaacac ttgtccaacg cgaatcaggt tggcgtttgt
      661 gatgccatta gcattaatga tcgtttggac agtcacacca taccgcaaag cgatggcata
      721 gagcgtatcc ccggctttga ctgtgtagct cgttcccgtt cctgttcccg gcgtgcttgt
      781 ggtgttcata agttgggaaa gggtcacaaa cgagtagccc atcgcacgca actgattgat
      841 gatgttcggc aatgcggcag tcgtcccgtt ggcgccgtaa ccggtgtgca tcagaacgat
      901 ggctccaggt acgatattat tgatgacgcg gttgtagatg tcagtggctg aattccctgt
      961 ccagtcgagc gaatcgatag tccactggaa cgtgtaagta tagccggcgt ttccgacagt
     1021 ttgtaggacg gtactgttat aggatccgaa tggagcgcgg aaaaatggtt ttgtgctctt
     1081 gccggtgaga ttcgtgatga tcgtttcggt tcgggtgagt tggtttgtca tttcggtcgt
     1141 tgctacggtt gtgaaatccg gatgattata ggagtggttt ccgatgtcat ggccagctga
     1201 gacaatgttc ttgattgcct gcggatgatt ttcagcacct tgaccggtga ggaagaaagt
     1261 ggcttttatg ttattggctg ataatgtgtt cagaattgat tggatgttat atccgtccga
     1321 tccatcatca aacgttaagg caacaacctt attggatgtc gaaccttgat aaataatttg
     1381 cgaggtagcg gccaaaacag gggttgccga aagggtgaag agaagcagga gcgaaaggat
     1441 gactccgaat aaacgtctga acgcctttct tgaggtgttc atttttattc ctcttttcta
     1501 ttgcagcctg agcgaaaggg ttgaagtgaa agccaagtgg cggtgatttg agattttgac
     1561 aaaaactgga ttggctcagt tcttttccac acttacattt taccataaac gttcaggtga
     1621 tggtatgaaa acgcatgcaa atcgatggga cgttcgacct ggaaatgtca gggattattc
     1681 tgaaattgtt aggattttgc aaaataagtc tactatagat tgaaaaaaaa cggatatttg
     1741 gtaaaatact tgagtgatgg tattattatt ctctttcatt catcaggaag aaagcaggtt
     1801 gcacatgtca acagatagaa atgtcaaaga agaaaaagaa acaacaaaca ataagatggt
     1861 acaggggact ttttggatga ctttcgggag cattttttcc cgattgatcg gcgcactcta
     1921 tatcatcccg
//
LOCUS       DAOMUJ010000361         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16786_length_1930_cov_1.817600, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000361 DAOMUJ010000000
VERSION     DAOMUJ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16786_length_1930_cov_1.817600"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..356)
                     /locus_tag="PLT73_08210"
                     /pseudo
     CDS             complement(1..356)
                     /locus_tag="PLT73_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180612.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(401..1471)
                     /locus_tag="PLT73_08215"
     CDS             complement(401..1471)
                     /locus_tag="PLT73_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM20394.1"
                     /translation="MRRSAILILFSTILGVFAFIVGGVLFALRPYLSYQELIEVDRFY
                     VPAFLYIIVLMLFAAIVVTWIAHLYLKNQENRIKDKLHWLYLGNYEHAVFNKKQDKSL
                     FLDQYCHAIHDEIEMLRKKMLSLSREVQQLSAHPQQVGNETKEEILEQERHRIARELH
                     DSVSQQLFAAMMMLSAVNERAEAFPEKIQKQMRLIEGIINESQSEMRALLLHLRPTKL
                     EGKSLKKGIEQLLVELKSKVQIAIKWEIDDVHTMSGIEDHFFRIVQELLSNTLRHAKA
                     SNLEVYLKQTFQEISLRVYDDGVGFDTSIEKSGSYGLMNIKERVQGMGGSLKIISFPN
                     KGTVIEIRIPNTNMNKNSASAE"
     gene            complement(1468..>1930)
                     /gene="liaF"
                     /locus_tag="PLT73_08220"
     CDS             complement(1468..>1930)
                     /gene="liaF"
                     /locus_tag="PLT73_08220"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HRM20395.1"
                     /translation="SELKVDSYVDVPWEEKAFRTVKTREPGQRSGTRKRQKWIGNTSI
                     GTDVYEWDDVNLTIFMGDTIIDLGNTLLPKEENIILIRKGFGKTRVIVPLGIGVSVHH
                     STIKGQLSFNEEEIDLTNEMVKLYSQGYDEANRKIKIVSNSLLGDLEVIYL"
BASE COUNT          472 a          485 c          401 g          572 t
ORIGIN      
        1 cgccgtggta agtggaacgg attgcggcgg caatttcatg cgctgaagag gttttcagca
       61 tgtaactgga agcccccgcc tccaatgccg ggtaaacctt ttcgtcatcg atgaagctgg
      121 ttacgatgat aatttttgct ttgggccatt cgttcagtat cgcttttgtc gaagcaatgc
      181 catccatgac ttccatgacc agatccatca aaatgatgtc cgggcgtaaa tccaaggcca
      241 tttcatagcc aatcaagccg ttttctgctt cccctactac ttcaatggat ggataaatag
      301 gccgaataac ccagccttac catctcgtga tcgtcgataa tcaaaacacg tatcaaactg
      361 aaccctcctc aagaaacttt tttcagaaat tctgcttatt ctattcagca cttgcgctgt
      421 ttttattcat attcgtattg gggatgcgga tttcgatcac ggtaccttta ttggggaaac
      481 tgatgatctt aaggctcccc cccatccctt gcacgcgctc ctttatgttc attaggccat
      541 agctgccgga tttttcaatt gaggtatcga atcctacgcc atcatcatat acacggagac
      601 tgatttcctg aaaggtttgt ttcagataga cttccagatt acttgccttt gcatgcctca
      661 gggtgttcga aagcaactcc tgcacaatac ggaaaaaatg atcctcgatg ccgctcatcg
      721 tgtggacatc atcgatttcc catttgatcg cgatttggac tttcgacttc aattccacca
      781 acaactgttc gatccctttt ttcagggatt taccttcgag tttggttgga cgaagatgca
      841 agagcaaggc ccgcatctcc gactgggatt cgttgatgat gccttcgatg agacgcatct
      901 gtttctgtat tttttccgga aaagcctctg ctcgctcatt gactgcggac aacatcatca
      961 tggcggcaaa gagttgttgg ctcacggaat catgcagctc ccttgcgatg cgatgccttt
     1021 cctgctcgag tatttcttct ttcgtttcat tgccgacctg ctgcggatgt gcgctcagtt
     1081 gctggacttc acgggacaag ctgagcatct ttttccggag catttcgatc tcatcatgga
     1141 ttgcatggca gtactgatcc aaaaatagtg atttatcctg ttttttgttg aataccgcat
     1201 gctcataatt gccgagataa agccaatgca gtttgtcttt aatacggttt tcttgatttt
     1261 ttaagtagag gtgggcgatc caggttacaa cgatagcagc aaacagcatc aatacgatga
     1321 tgtaaagaaa cgccggcaca taaaaacgat ccacctcaat cagttcctgg tagcttaagt
     1381 agggcctaag ggcaaataaa acaccaccca cgatgaaggc aaaaacgccg agtatcgtgc
     1441 tgaacagaat caaaatagcg cttctcctca taggtagatc acctccagat cacccagcag
     1501 actgttggac actattttga tctttctgtt tgcctcatca tagccctgac tgtacaattt
     1561 gaccatttca ttggtcaagt caatttcttc ttcgttgaat gatagctggc ccttgatggt
     1621 ggaatgatga acagacacgc cgattcccaa cggtacgatt accctggtct ttccgaatcc
     1681 cttgcggatc aggatgatat tttcctcttt cggcagcagc gtattcccta aatcgatgat
     1741 tgtatcgccc atgaatatag tcagattgac atcatcccat tcatagacat ccgtcccgat
     1801 actggtgttt ccaatccatt tctggcgctt tctggtaccg ctccgctgtc ccggttctct
     1861 cgttttgaca gtacggaatg ctttttcttc ccaaggaacg tccacatacg aatccacctt
     1921 caactcagac
//
LOCUS       DAOMUJ010000362         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16840_length_1925_cov_1.760963, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000362 DAOMUJ010000000
VERSION     DAOMUJ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16840_length_1925_cov_1.760963"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..>542
                     /locus_tag="PLT73_08225"
     CDS             362..>542
                     /locus_tag="PLT73_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRM20396.1"
                     /translation="MESEMKTKTKKGVTLLVEALKNEGVEVLFGYPGGAVLHIYDEFY
                     KSESNHVLTRHEQGAV"
     assembly_gap    543..552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <553..>1925
                     /gene="ilvB"
                     /locus_tag="PLT73_08230"
     CDS             <553..>1925
                     /gene="ilvB"
                     /locus_tag="PLT73_08230"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRM20397.1"
                     /translation="ARATGKPGVCIVTSGPGATNALTGIATAQMDSIPLIVITGQVHE
                     AGIGKDAFQETDMIGMTTPVTKYNYQVRDAKDIPRIIHEAFYLANTGRKGPVVIDIPK
                     NIGVQEVECEDCTENIGPVRNLPGYNPECLPVPEQVAKVNEAIAASKKPIILAGQGVI
                     HAQAEKELLAFAEYYQIPVVNTLLGLGSFPVKHELALGMGGMHGSYASNMALMECDLL
                     LNFGARFDDRVASDPTNFAPEAVIVHVDIDNAEIGKIMHTDIPVLSDAKLALLMLNDT
                     KLAEVPDHSVWFNHVMDNKKQHPFRYEKSDTFIKPQHVVEYIGELTNGEAIVVTDVGQ
                     HQMWAAQYYPFTRGKQLLTSGGLGTMGFGIPAAIGAKMAYPDKTVVCFVGDGGFQMTN
                     QELAILNANRLDVKYFILNNEVLGMVHQWQNKFYKGRYSHSEIPDSPDFVKLADAYGI
                     TAARVSD"
BASE COUNT          565 a          421 c          448 g          481 t
ORIGIN      
        1 gaagcgcaca aaaaaatcgt ccggcacgtt catgttggac aattattaga aataagtgaa
       61 aataatatga aaattaccgt tgacactctc atgtaaatct tatatgatta tatcaatcac
      121 ttaagcaata acaaatccat atagaaatca ggtatcccat atgaaaacga tgaatccact
      181 aaacattatt atcgtcttaa tgatctagga gttaaacact gtagaaattt tttacagatt
      241 gtttaagtcc tatttgtggt aatcaaatgg gatgaagcat cccatcattg actgatgagg
      301 atgctttttt ttattcatat tgcggacagt cataggaaaa acgagaaatg gggagataaa
      361 aatggaatcc gaaatgaaaa ctaaaacaaa aaaaggcgta acccttcttg ttgaagcgtt
      421 gaaaaatgaa ggtgtcgaag ttctgtttgg ttacccaggc ggcgctgtcc tgcacatcta
      481 tgatgaattc tacaaatccg aatcgaatca tgttttgacg agacacgagc aaggcgcggt
      541 ccnnnnnnnn nnatgcccgt gctaccggca aacctggcgt ctgtatcgtt acaagcggac
      601 ccggcgcaac gaatgctttg accggaatcg cgaccgctca aatggattcc attccattga
      661 ttgtgatcac tggccaagtg catgaggcag gcatcggtaa agacgctttc caggaaactg
      721 acatgattgg catgaccaca cctgttacaa aatacaacta tcaagtgcgt gatgccaaag
      781 atatcccgcg catcatccac gaagctttct acctggcgaa tacaggcaga aaaggaccgg
      841 ttgtcatcga tatcccgaaa aatatcggtg tccaggaagt ggaatgtgaa gactgcacag
      901 aaaatatcgg tccagtaaga aacctgccgg gctacaatcc ggaatgtttg cccgtaccgg
      961 aacaggtcgc aaaagtgaac gaagcgattg cagccagcaa aaaaccaatc attttggccg
     1021 gtcaaggcgt tattcacgca caagctgaga aagaactgtt ggcttttgcg gaatactacc
     1081 aaattcccgt tgtgaacacg ttgttgggtc tgggaagctt ccccgtcaaa catgagcttg
     1141 cgttaggtat gggcggcatg cacggctcct acgcctcaaa tatggctttg atggagtgcg
     1201 acttgcttct caacttcggc gcacgtttcg atgaccgtgt cgcaagcgat ccaaccaatt
     1261 ttgccccgga ggctgtcatt gtccatgttg acatcgataa tgccgaaatc ggcaagatca
     1321 tgcacactga tatcccagta ttgtctgatg ccaaattggc gttgctgatg ctgaatgata
     1381 cgaagctggc agaagtgcct gatcactcgg tttggttcaa tcacgttatg gacaacaaaa
     1441 agcaacaccc tttccgttac gaaaaatccg atacgttcat caaaccgcaa catgtagtcg
     1501 agtatatcgg cgaattgacg aatggtgaag ccattgtcgt aacggacgtt ggccagcacc
     1561 aaatgtgggc ggctcaatat tatcctttca cgcgtggtaa acaattgctg acaagcggtg
     1621 gcttgggaac gatgggcttc ggtattccgg cagctatcgg tgctaaaatg gcttaccctg
     1681 ataaaacggt tgtctgcttc gtcggagacg gcggcttcca gatgaccaac caggaattgg
     1741 cgattctgaa cgccaatagg ctggacgtga agtactttat tcttaataac gaagtactgg
     1801 gcatggttca tcaatggcag aacaaatttt acaaaggccg ttactctcat tccgagattc
     1861 cggattcacc tgattttgtt aaacttgctg atgcttacgg catcacagct gcgagggtat
     1921 ccgat
//
LOCUS       DAOMUJ010000363         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16854_length_1924_cov_1.949171, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000363 DAOMUJ010000000
VERSION     DAOMUJ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_1924_cov_1.949171"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1628)
                     /gene="zwf"
                     /locus_tag="PLT73_08235"
     CDS             complement(123..1628)
                     /gene="zwf"
                     /locus_tag="PLT73_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRM20398.1"
                     /translation="MVKTNPVLIVLFGATGDLAQRKLYPSFFRLFRSGALAERFAVIG
                     TARREWSDAHFRDVVSDSIQSFQPTDAEKAAFLSHFYYRAHDVSDVEHYVHLKELADK
                     LDAKYDLDQNRMYYLAMAPQFFGPIVTHLKGQQIVTDKGFNRVVIEKPFGMDYASAEK
                     LNNEITKVFPEEDIFRIDHYLGKEMIQNILAVRLTNPFLEPSWNREYIENVQITFSEE
                     LGVEERGGYYDHSGALKDMVQNHILQVLSLLAMEPLASVSDANIQTAKIKALNDVRVY
                     SPEEVRENFVRAQYAAGRLGDDGFVAYQEEPNVASDSRTETFVAGKFMVDNPRWKGVP
                     FYVRTGKRMTEKGTRVNIVFKKVPVNPFDAEKDVPANDLTIYIQPTEGFALTMNRKEI
                     GQGFNTQPIKLEYRHDSEMMENTPEAYERLTYDVILGDATNFARWEEVAQSWRIIDAI
                     RETWDSDNEEIPKYAARTMGPKEAFDLLKKENHEWVWTPDEWYRERGLLKG"
     gene            complement(1731..>1924)
                     /locus_tag="PLT73_08240"
     CDS             complement(1731..>1924)
                     /locus_tag="PLT73_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRM20399.1"
                     /translation="ALQSGSNEKYMVSQKGEPFIGAQVSRQAVARLIMYIIGNQAVYS
                     NKSLGVSEPNTDFPKPSFY"
BASE COUNT          474 a          511 c          385 g          554 t
ORIGIN      
        1 tccgttcgtt aggctgtcct ccggttaacc ggtccaaacc ggagaacagc cccgatatct
       61 tcaatctcct ccggccagca gctactcccc ggaggagagc catccacaca tcgataccga
      121 atctatccct tcaacaatcc gcgttcgcgg taccactcat ccggtgtcca gacccactcg
      181 tggttttctt ttttcaacaa atcaaatgct tcttttggtc ccattgtcct tgccgcatac
      241 ttcggaattt cttcattgtc gctgtcccac gtttcgcgga ttgcgtcaat gattctccag
      301 gattgtgcaa cttcttccca acgcgcaaag ttggttgcat cacccaaaat aacatcgtaa
      361 gtcagacgtt cataagcttc cggcgtattc tccatcattt cgctgtcgtg gcggtactcc
      421 aatttgatcg gctgcgtatt gaatccctga ccgatttctt tccggttcat ggtcaaagca
      481 aagccttccg ttggttggat gtagatggtc agatcgttcg ccgggacatc tttttcagca
      541 tcaaacggat taacgggaac ttttttgaaa acgatattga cgcgcgttcc tttttccgtc
      601 atccgtttgc cggtgcgcac atagaaaggc acacccttcc agcgtggatt atccaccatg
      661 aacttcccgg ctacaaacgt ttccgtcctc gaatcgcttg cgacattcgg ctcttcttga
      721 taggcaacaa atccatcatc acccaaacgg cctgccgcat actgcgctct cacgaagttt
      781 tcgcggactt cttccggtga atagacacgc acatcattca gggctttaat cttcgctgtt
      841 tggatatttg catccgatac ggaagctaat ggttccatcg ccaacaaaga cagtacttgc
      901 agaatatggt tttggaccat atctttcagg gcgccgctat ggtcatagta gccaccgcgt
      961 tcttccacgc ccagttcttc ggaaaaagtg atctgaacat tttcgatgta ctcacgattc
     1021 cacgagggtt caaggaacgg attcgtcaaa cggacggcca aaatattttg gatcatttct
     1081 ttacccaaat agtggtcgat cctgaaaata tcctcttccg ggaacacttt tgtgatttca
     1141 ttgttcaatt tttcggctga tgcataatcc atcccgaatg gcttttcgat gacgacgcgg
     1201 ttgaaccctt tgtccgtcac gatttgttgc cctttcagat gcgtcacgat cggtccaaaa
     1261 aactgcggcg ccatcgccag ataatacatc cggttctgat ccaaatcata tttcgcatcc
     1321 aatttgtcag ccaactcttt gaggtgaaca taatgctcca catcggaaac atcatgcgca
     1381 cgataataaa agtggcttaa aaaggcagct ttttccgcgt ctgtcggctg gaatgattga
     1441 atagaatccg aaacaacatc ccgaaaatgc gcgtcgctcc attctcttct tgccgtcccg
     1501 atcactgcga aacgttctgc caacgcaccg cttcgaaaca atcggaaaaa tgaaggatat
     1561 aactttcttt gagctaaatc acctgtcgct ccgaataaaa caatcaatac tggatttgtt
     1621 tttaccactc tatcgcctcc tgcgttactt aatatttcca taataccatg attagcagaa
     1681 agttccttta ctcttggaga atttaaacac ctaaaaaatc aaaaacattt ttaataaaag
     1741 gatggtttcg gaaaatctgt gttgggttcg ctgacgccca gacttttgtt tgaatagaca
     1801 gcctgatttc caatgatgta catgatcaac cgcgcgactg cttggcggga cacttgcgca
     1861 ccgatgaaag gttccccttt ttggctgacc atgtactttt cgttgcttcc tgattgtaaa
     1921 gcca
//
LOCUS       DAOMUJ010000364         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16922_length_1918_cov_1.940955, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000364 DAOMUJ010000000
VERSION     DAOMUJ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_1918_cov_1.940955"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>535
                     /locus_tag="PLT73_08245"
     CDS             18..>535
                     /locus_tag="PLT73_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20400.1"
                     /translation="MKEQHNRTPREIVKELDRYIIGQDAAKKAIAVALRNRYRRQKLD
                     ADMQKEVTPKNILMIGPTGVGKTELARRLALLVEAPFVKVEATKFTEVGYVGRDVESM
                     VRDLMENAIQIVEKQKRSDVYAKAYEAALERLSKVMRPGTKKAKPKQEGMNDWQNMFK
                     NLGMPLPESQEE"
     assembly_gap    536..545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            546..1273
                     /locus_tag="PLT73_08250"
                     /pseudo
     CDS             546..1273
                     /locus_tag="PLT73_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780934.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1284..1429
                     /locus_tag="PLT73_08255"
     CDS             <1284..1429
                     /locus_tag="PLT73_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HRM20401.1"
                     /translation="TIMEKLLEDLLFEASEIPGTEITITEHYVDEKLEKIVENKDLRR
                     YIL"
     gene            1502..>1918
                     /locus_tag="PLT73_08260"
     CDS             1502..>1918
                     /locus_tag="PLT73_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052909.1"
                     /note="CodY; DNA-binding protein that represses the
                     expression of many genes that are induced as cells make
                     the transition from rapid exponential growth to stationary
                     phase (By similarity). It is a GTP-binding protein that
                     senses the intracellular GTP concentration as an indicator
                     of nutritional limitations. At low GTP concentration it no
                     longer binds GTP and stop to act as a transcriptional
                     repressor; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRM20402.1"
                     /translation="MDELLEKLRRINHMLQKEGGFVTNSGEATALPFTEMASVLGDIL
                     RANTYLIDLSGNLLGYSEATDINNTRIKQMLEDKKFPEQYAQNLSALFQTTANIGIES
                     DFTAFPIESRDLFITGVTTIVPIFASGKRLGSLILAR"
BASE COUNT          607 a          393 c          470 g          428 t
ORIGIN      
        1 gataggtggc taatataatg aaggaacagc acaacagaac acccagagaa atcgtcaagg
       61 agctcgatcg ttatatcatc ggccaggatg ccgcaaaaaa agcaatcgct gtcgctttgc
      121 ggaatcgcta ccggagacaa aaattggatg ccgatatgca aaaagaagtg acgccgaaaa
      181 atattctgat gatcgggccg accggggtag gaaagaccga gctggcgaga cgcttggcct
      241 tattggtcga agcacccttc gtcaaggtcg aggccactaa attcacagag gtcggctatg
      301 tcggccgcga cgttgagtcg atggtccggg acctgatgga gaacgccatt cagattgtcg
      361 aaaagcagaa gcgtagcgat gtctatgcga aagcctatga ggctgcttta gaaaggctct
      421 caaaggtaat gaggccgggt accaaaaaag ctaagcccaa acaggaaggt atgaatgact
      481 ggcaaaatat gttcaagaat ctagggatgc ctctgccgga aagtcaggaa gaagannnnn
      541 nnnnnctacg aagactacga ggaagaagaa gttgaagaag tgaccgccga aatcgccaag
      601 agcagagcgg aaatcattga acaattgcgt aaaggacttc tgaataagcg tgaagtctcc
      661 ataaaagtgg aggaaaaaca agcaaatccc ttaggtggcg gcggaaacaa cgaacaaatg
      721 atgatgcttc agtcggcatt cgaatccatg acgccgaaaa agaaaatcca gcggacactt
      781 ttggtcgaag atgctatcga ggtacttgtg caggaagaaa ccgacaaact agtcaacaag
      841 gatgatattt cgcaggaagc tttgaagttg gccgaaacca acggcatcat tttcatcgat
      901 gaaatcgata agatcacttc caaatcacaa aattcaggtg aagtgtcgcg ggaaggtgtg
      961 caacgggata tccttccgat tgtcgaagga agccaggttt ccaccaagta tgggacactc
     1021 caaacagatc acatcctctt catcgcatcc ggcgcgttcc atgtttccaa gccaagcgat
     1081 ctcatcccgg aactgcaagg tcgtttcccg atccgcgtag aattaaatga ccttacggaa
     1141 gatgatttcg tcagaatcct taccgaaccc aataatgcga tgctaaagca gtacactgcg
     1201 ttgttggcaa ctgaagacgt cgatatcact ttcacccagg aagctatccg gaagatggct
     1261 gccatcgcga ctgnnnnnnn nnnatacgat catggaaaaa ttattggagg atttgttgtt
     1321 cgaagcttcc gaaatacctg gtacagaaat taccataaca gaacattatg tcgatgaaaa
     1381 gcttgagaaa attgtagaaa ataaagattt aagaagatat atcctttaag cataaataat
     1441 ccccttcagg atagataaac cttgtggata ttttctgatg gatgatcgta tggggggaaa
     1501 catggacgaa ttattagaaa agttgagaag gatcaaccac atgcttcaaa aagagggtgg
     1561 gtttgtcact aacagtggcg aagcgacagc attgcctttt acggaaatgg caagcgtact
     1621 tggtgatatt ttaagagcca acacgtatct gatcgatcta tccggaaatc tattgggcta
     1681 cagcgaggcg accgatatca acaatacccg catcaaacaa atgctggagg ataagaaatt
     1741 tccggaacag tatgcccaaa atctctctgc tttatttcaa acgaccgcga atatcggcat
     1801 tgaatctgat ttcactgcct ttccgattga gagtagagac ttgtttatta caggtgtcac
     1861 aacaatcgtt ccgatttttg cttccggcaa gcgtttgggg agtttgattt tggcacgt
//
LOCUS       DAOMUJ010000365         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_16947_length_1916_cov_1.929608, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000365 DAOMUJ010000000
VERSION     DAOMUJ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16947_length_1916_cov_1.929608"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLT73_08265"
     CDS             <1..929
                     /locus_tag="PLT73_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM20403.1"
                     /translation="FIELLEHRLNIKRPIGILIVMLLLIGIVIFSFLALILNLVNQIG
                     ELLTNLPGVIRSLNNVYKQVIEQEWVARLDIPTQLSQLSGNIQSSITAFLNGLTGSIG
                     SVLSAVANIALLVVIIPLIFFYMLKDGHKFPEAVAQLLPQEYSADVLALLKEINKTIA
                     KYISGQALVCLFVGTFTFIGYLLIGLPYAFLLGVTAAVTNIIPYLGPYIGLIPAAIIG
                     LTVSPAKALLVCVVVLVVQQVESNIISPNVLGKTLDMHPLTILFLLLAVGKISGVLGM
                     ILAIPTYAVVKTIVQYVHRYVKNRKKKVLYKE"
     gene            1110..>1916
                     /locus_tag="PLT73_08270"
     CDS             1110..>1916
                     /locus_tag="PLT73_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20404.1"
                     /translation="MKVIVVGCTHAGTSAVKTILKENPGTEVTVYERNDNVSFLSCGI
                     ALYVGGVVKDPQGLFYSNPEELASLGANVNMQHDVTNIDTAAKTVSVKNLVTGEEIQD
                     TYDKLVLTTGSWPIIPPIEGIQSKNVLLCKNYNQAKEIIARKDDKKKIVVVGGGYIGI
                     ELVEAFANDGKEVTLVDGLDRILNKYLDTEFTDILEDDLRAHGVKVQLNEMVKGFKDN
                     EAGDITTVVTTGGEYDAELVILCVGFRPSTELVKGQVEMMGNGAIIVNDYM"
BASE COUNT          559 a          368 c          443 g          546 t
ORIGIN      
        1 cgttcatcga actgcttgag cacaggctga acatcaaacg tccgataggc atcctgatag
       61 tcatgttgct tctgatcggc atcgtcatct tctcgttttt ggcgctcatt ctgaatcttg
      121 taaaccagat cggagagttg ctgacaaatt tgcctggtgt tatccgttcc ctgaataatg
      181 tctataagca agtgattgaa caggaatggg ttgcgcggct ggacattccg acccaattgt
      241 cccagttgag cggaaatatt caaagcagta tcaccgcctt tctcaatggc ctgactggca
      301 gcatcggttc tgttctgtcg gccgtagcca atattgcctt gcttgtcgtc atcattccgt
      361 tgattttttt ctatatgctg aaagatggac ataagtttcc ggaagcggtc gctcaattat
      421 tgcctcagga gtattcagca gatgttttgg cgctcctgaa ggaaatcaac aaaacaatcg
      481 ccaaatatat cagcggacag gcactcgttt gtttgttcgt agggacattc acctttatcg
      541 gatatctact gatcggattg ccttacgcct tcctgcttgg agtcactgca gccgtcacaa
      601 acattattcc ttatcttgga ccctacatcg gcttaattcc ggccgcaata attggattga
      661 cagtctcgcc ggcgaaagcg ctcttggtct gtgtggttgt cttagtagta cagcaagtgg
      721 aatcgaacat catttcacca aacgtcttgg gcaaaacatt ggatatgcat ccgctgacga
      781 ttctcttttt gttgttggct gtcggcaaga tttccggtgt tctgggaatg atattggcga
      841 ttcctacgta tgcagtcgta aaaaccatcg ttcaatatgt ccacagatac gttaaaaaca
      901 ggaagaaaaa agtgttatat aaagaataat gaaaattaat atggattcca agggttattg
      961 attgcttttt acctttggaa tcctttatta tataaaaagt actaagagca taacgaatca
     1021 atttgtgcag tttttattat gaattaagag aatcgttgcg gttgatttgt gcaaaatata
     1081 ttaatgcgga aaatttagga ggaagtacaa tgaaagtaat cgtagtggga tgtacacatg
     1141 ccggaacatc agcagtgaag acaattttga aggagaatcc agggactgaa gtaacggtat
     1201 acgaaagaaa cgataacgtt tcgttcttgt catgcgggat tgcgctttac gtcggcggtg
     1261 tcgtgaaaga tccacaagga ttattctatt ctaaccctga ggaacttgct tcgttgggcg
     1321 caaacgtaaa catgcaacat gatgtaacaa acattgatac agcggcaaaa actgtttctg
     1381 tgaaaaattt agttactggg gaagaaatcc aagacactta tgataaattg gttctgacta
     1441 ctggttcatg gccaatcatc ccgccaatcg aaggcatcca gagcaaaaat gttttgcttt
     1501 gcaaaaacta caaccaagca aaagaaatca tcgcacgtaa agacgataaa aagaaaatcg
     1561 ttgttgtcgg cggcggttac atcggtatcg aattggtgga agcatttgcc aacgatggaa
     1621 aagaagtaac attagttgat ggtttagatc gcattctgaa taaatatttg gatactgaat
     1681 tcacggatat ccttgaagat gacttgcgcg cacatggggt aaaagttcaa ttgaacgaaa
     1741 tggttaaagg tttcaaagac aacgaagcgg gtgacatcac taccgtagtt actactggcg
     1801 gagaatatga cgctgagttg gtcattcttt gtgtcggatt cagaccgagc actgagcttg
     1861 tcaaaggcca agtggaaatg atgggaaatg gcgcaatcat cgtgaatgat tacatg
//
LOCUS       DAOMUJ010000366         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17088_length_1904_cov_2.201190, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000366 DAOMUJ010000000
VERSION     DAOMUJ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17088_length_1904_cov_2.201190"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLT73_08275"
     CDS             complement(<1..530)
                     /locus_tag="PLT73_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM20405.1"
                     /translation="MSDYIENPELNEEESNNVEQTMQDVLDEALSIEVGDTVKGEVLS
                     IQDRQAIVGIIGGGVEGVIPYNELSAKPFDEVTEILNVGDVVDLVVIKQIKDKENGSF
                     LLSKRRIDAKIVWKDIQDKFENNEIIEAPVKDVVKGGLVVDVGVRGFVPASMVEDHFV
                     EDFSSYKGKVMTFKIV"
     gene            complement(688..1371)
                     /gene="cmk"
                     /locus_tag="PLT73_08280"
     CDS             complement(688..1371)
                     /gene="cmk"
                     /locus_tag="PLT73_08280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRM20406.1"
                     /translation="MNIAIDGPASAGKSTIAKKVAEQLGYIYLDTGAMYRTLTYAALS
                     NEVDLQDEEALHTLLKGIRITFLTAENEMQRVFLNDEDVTDSIRSEEVTQNVSLVSSF
                     AKVREEMVARQKSIARSGGVVMDGRDIGTVVLPDAEVKIFMTATAEERALRRYKENIA
                     KGMTTSLEELTEDMKRRDHLDSTRSVSPLKKAEDAIVLDSTHLEIDEVVKQILGIIEV
                     SLNPLQTLD"
     gene            complement(1445..>1904)
                     /locus_tag="PLT73_08285"
     CDS             complement(1445..>1904)
                     /locus_tag="PLT73_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM20407.1"
                     /translation="LKVLLFLFLAILIIPFATIYYNNQNQNTPVPKSAEQVMINKRVA
                     SSSVESSEEVSSEEPEASSEVAESEAEPESTIVETPSSSTVPPVVETPVETVEEPATT
                     NGAYNNSYTIKAGDNLYRIAVNHNMTLDELMQANGLSSTEAQIGMVLNVK"
BASE COUNT          463 a          470 c          356 g          615 t
ORIGIN      
        1 tcaacaattt tgaatgtcat tactttacct ttatatgaac tgaaatcttc aacaaaatga
       61 tcttcaacca ttgacgccgg aacaaaacct ctaacgccga catcaacaac caatccgccc
      121 ttaacgacgt ctttgactgg cgcttcgatg atttcgttgt tttcaaattt atcttggata
      181 tctttccaaa cgattttagc atcaatgcga cgcttcgaaa gcaagaaact tccgttttct
      241 ttgtctttga tttgtttgat gacaaccagg tccacaacgt cacctacgtt taggatttct
      301 gtaacttcat cgaatggttt tgcggataat tcgttgtaag gaataactcc ttcaactccg
      361 cccccgatga ttcctacgat ggcttgtcta tcttggatgc ttaaaacttc tcccttgaca
      421 gtatcaccca cttcaatgga taatgcttca tccaacacat cttgcatagt ctgttcaacg
      481 ttgttggact cttcttcatt taactctggg ttttcaatgt aatctgacat gtaactattc
      541 ctcctaaaag tctgcggcaa ccgattggct aggctcctta actccctcgc cttccggctt
      601 aggaactaat ccttgactcc atatttttga aagcgtaata ctgcatataa tcattctaac
      661 atttttgctt aaaatcgtct agtattgtta atccaatgtt tgtaaggggt ttagactcac
      721 ttcgataatg ccgagaatct gcttaacgac ttcgtcgatt tccaaatgtg tagagtccag
      781 aacgatggca tcttccgcct ttttcaatgg tgaaaccgac cttgtgctat ccaaatgatc
      841 gcggcgcttc atgtcttccg tcagttcttc caaggaagtg gtcataccct tcgctatgtt
      901 ttctttatag cgtcgcagcg ctctttcctc tgcagttgct gtcatgaaaa tcttgacttc
      961 cgcatcaggc aatacgacgg tacctatgtc cctgccgtcc attacgaccc cgccggagcg
     1021 tgcgatactc ttttggcgag caaccatctc ttcacgcact ttcgcaaacg aggaaaccaa
     1081 ggagacgttt tgggtcactt cctccgatct gatcgaatcg gtcacgtcct catcattcaa
     1141 aaacacgcgt tgcatttcgt tttcggcagt caaaaaagtt atcctgatcc ctttcaataa
     1201 cgtatgcaat gcctcttcat cctggagatc gacttcgttg gataaagctg cataagtcaa
     1261 cgttcgatac atcgcacctg tgtccagata gatgtatccc aattgttccg caactttctt
     1321 cgcgatggta cttttaccgg ccgatgcggg accatctatg gcaatattca tttttttcac
     1381 attaaacctc cgactttgtt ctatctgatt tcattgaaaa agccgcctta tcagacggct
     1441 tccattattt aacgttcaat accataccga tttgcgcttc tgtgctgctc aaaccgttag
     1501 cctgcatcaa ctcatccaat gtcatattat ggttcacagc aatccgataa aggttatcgc
     1561 ctgctttgat ggtgtaggaa ttattgtacg ctccgtttgt tgttgcgggt tcttcgactg
     1621 tttcaacagg cgtttcgacc actgggggta cagtgctgga acttggcgtt tccacaatcg
     1681 ttgattccgg ttctgcttcg ctctcagcga cttcactgga tgcttcgggc tcttcagaag
     1741 atacttcttc gctgctctcg acactggaac tggctactct tttgttgatc atcacttgtt
     1801 cagcgctctt gggtacggga gtattttgat tctggttatt ataatagatg gttgcaaaag
     1861 gaataatcag aatagccaaa aagagaaaca gcaatacttt taat
//
LOCUS       DAOMUJ010000367         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17095_length_1904_cov_1.636560, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000367 DAOMUJ010000000
VERSION     DAOMUJ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17095_length_1904_cov_1.636560"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLT73_08290"
     CDS             complement(<1..350)
                     /locus_tag="PLT73_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HRM20408.1"
                     /translation="MEESISVLQQIVPDIMSVLQNRYHIMRNIQMYAPVGRRVLADKV
                     SLSERTLRTETDFLRKQCLLSSSKNGMELTEEGENVYRQLERFMRLLLNMNAKEKALA
                     DYLNIQFCRIVPGD"
     gene            complement(719..1813)
                     /locus_tag="PLT73_08295"
     CDS             complement(719..1813)
                     /locus_tag="PLT73_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20409.1"
                     /translation="MLVISSVHMDKESLEESLKLVGYDNEELFAYLQQKALGNPLIEV
                     KINKEFDERAVKEKTVVYKPDKDDPMDTASAMDQVSEEVPALDTFLLEQIYANMRDTP
                     LRDLVLFLVAFINPEGYVTINLNEAAEQKGVEPIEMLDALTLLHQLDPPGVGARNLQE
                     CLMLQTERDEYAPEIAYYVLENFFTAFSDKNWQEIADEMAVDLADVKSVFEYVQSLDP
                     TPGSAFGDDNLFLPRPDLYLQLLDGHLTVKYNEWASPFVVFQKEYYEEMLQHDDEDVQ
                     QFLAAKKTEGEQLRSALAFRKEMLLAAGELICSLQKGFFLEGRELGPLDVTRLAEDLA
                     VPVTVMREEILEKMIETDRGTFLLHQLCGE"
BASE COUNT          494 a          500 c          342 g          568 t
ORIGIN      
        1 ctgtcaccag gaacaatcct acaaaactga atgttcaaat aatccgctaa cgccttttct
       61 ttcgcattca tattcagcaa tagccgcatg aatctttcca gctgacggta aacattttcg
      121 ccttcctcgg tcaattccat cccgttcttc gaactggaaa ggagacattg tttccgcaaa
      181 aagtctgtct cagtccgcaa agtcctttct gataaggaaa ctttatcggc caggacacgt
      241 ctcccgactg gagcgtacat ttggatatta cgcatgatat ggtaccgatt ttgaaggact
      301 gacataatgt ccgggactat ttgctgcaag actgaaatag actcttccat aacaaccctc
      361 ttcatttgca cgtggacgct tttcgccccg ctgcaatctc aggcggtcct cgtttatttc
      421 atgtcaggag agatggaata agactgtaaa tattttttta cttccgtcta ttctccctcg
      481 tctttacttc attattttaa cagtaaacac tagacgtttc aagagttttg ataaattaat
      541 tcaaattcct tcaaaaagga ttcgttcaat atatcacgag tagcctataa tccgattgcc
      601 tttcggatgt acgctttcaa aaaaccttga tacaacagcc attcaaaaag ggtcgtttta
      661 tgtcccgtta ttgtttagcg cttcgcatct ttttacttat cttaaccgct tttcgtggct
      721 attcgccgca taattggtgc aacaaaaatg tcccacggtc cgtttcaatc attttctcca
      781 atatttcttc gcgcatcaca gtgaccggaa ccgccaaatc ttcagccaag cgagtcacat
      841 cgagcggccc cagttctcgg ccttccagga aaaagccttt ctgcaaggaa cagatcaatt
      901 ctcccgccgc aagcaacatt tcttttctga atgccagcgc cgaccgcagt tgctcccctt
      961 ccgttttctt cgctgccaaa aattgttgta cgtcttcatc atcatgctgc agcatctctt
     1021 cataatattc tttttgaaaa acaacaaatg gagaagccca ctcattgtat ttcacagtca
     1081 ggtgcccatc cagcagttgc agataaagat ccggtctcgg caaaaataga ttgtcatcgc
     1141 cgaatgcgga tcccggtgtc gggtccaacg attggacata ctcgaataca cttttcacgt
     1201 cagccagatc aactgccatt tcatccgcta tttcttgcca gtttttatca ctgaaagcag
     1261 tgaagaaatt ttccaagaca taataggcga tttccggcgc gtattcatcc cgctccgtct
     1321 gcagcatcag acattcctgc agattccggg cacccacacc tgggggatcc aattggtgca
     1381 gaagcgtcaa tgcatccaac atctcaatcg gttcgacgcc cttctgttcc gcggcttcat
     1441 tcagattgat ggtgacgtac ccctccggat tgatgaacgc caccaagaat aacaccaaat
     1501 cgcgcaaggg tgtatcccgc atatttgcat agatttgttc caataaaaaa gtatccaacg
     1561 ccggcacttc ttcagacact tgatccattg cggaagcagt gtccataggg tcgtccttat
     1621 ccggcttgta tacaaccgtt ttttctttta cagcgcgttc gtcgaattct ttatttattt
     1681 tcacttcgat caaaggattg cccaacgctt tttgttgaag ataagcgaac aactcctcgt
     1741 tgtcgtatcc cacaagcttc agtgattcct ctaagctttc cttatccata tgcacacttg
     1801 atataactaa cacatcaacc cctcctcccc ttttcagaat cattttatca cacacaagca
     1861 tttattgaaa tcgttttcag ttaaggggat tggatttcaa tcgc
//
LOCUS       DAOMUJ010000368         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17114_length_1902_cov_1.240931, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000368 DAOMUJ010000000
VERSION     DAOMUJ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17114_length_1902_cov_1.240931"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLT73_08300"
     CDS             complement(<1..682)
                     /locus_tag="PLT73_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRM20410.1"
                     /translation="MELENDPERVSILDEIFRSAHTLKGMAATMGYTTMAELTHKMEN
                     VFELLKTGKLAADEGIVTLIFDCLDMLSAIVEDLRAENEGEQDTTTLKNKLDNLLAST
                     KGAPKDEIVSEAKLNETTIDDSNFALEKLDSSDISVIRDAHEKNYHAFFIKVSLDETS
                     MMKNARVYVVINKLEKSGDILYSDPDVVTMENEDFGNEFTIVYLTKTEKTAVEEQILE
                     TSEIEGVVV"
     gene            complement(802..1572)
                     /locus_tag="PLT73_08305"
     CDS             complement(802..1572)
                     /locus_tag="PLT73_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRM20411.1"
                     /translation="MLDFNWFYAWSEKTLHLNLIAYKEGQLQRRIETIMKKSGATDLK
                     QFAKMIETDDNIKKSFLDYITINVTEFYRNKEIFEEFEEVFITRLSSKFKALKVWSAA
                     CSTGAEAYSIAMILKKNMLTANSKIIATDIDETILSKAKAGTYSKLEVQNVPPMEKRL
                     YFTEQNNRYVLSDDIKKTVHFKKHDLILDSYEKGYHTIVCRNVTIYFKNEVKEKIYQN
                     IADSLVPGGLFFIGATESVYKPEQYGLKKVAAFLYEKV"
     gene            complement(1573..>1902)
                     /locus_tag="PLT73_08310"
     CDS             complement(1573..>1902)
                     /locus_tag="PLT73_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HRM20412.1"
                     /translation="HMTVKNRRIQLSQADKIHGVRPAVAHLFSSAAESYGSRLIGVVL
                     TGMGKDGTEGLKKIKTNGGYTFAQDKESSLGFGMLGNAIQNTVIDEVVNLEELTDALN
                     QVLGGRK"
BASE COUNT          447 a          458 c          363 g          634 t
ORIGIN      
        1 gaaccactac gccttctatt tcactggttt ccagaatctg ttcttcgaca gccgtttttt
       61 ctgtcttagt caggtagacg atggtgaact cattcccaaa atcttcattc tccatcgtca
      121 caacatcggg atcggaatag agaatatctc cgctcttctc cagtttattg atgaccacat
      181 agactctggc attcttcatc atgctggttt catcgagact gaccttgata aaaaatgcat
      241 ggtagttttt ctcgtgggca tcgcggatga ccgaaatatc cgaggaatca agtttttcca
      301 atgcaaagtt cgaatcatca atcgtcgtct cgtttaactt ggcttcgctg actatctcat
      361 ccttcggtgc tccttttgtg cttgccaaaa gattgtccaa tttgtttttg agcgttgtag
      421 tatcctgctc cccttcattc tcagctctca gatcttccac aatagcggag agcatatcca
      481 gacaatcgaa gatcaacgta acgatccctt cgtctgcagc taacttgccc gttttgagca
      541 attcaaacac gttttccatt ttgtgcgtca attcggccat ggttgtgtag cccatagttg
      601 cagccatgcc tttaagcgta tgcgcagaac ggaaaatctc gtccaaaata ctgactcttt
      661 ccggatcatt ttccagctcc atcacttggt cgttcaaatt ttgcagatgt tcatcggtct
      721 cttaaaagaa gagttccctg tatttactgt tatcgtccat tgaatcccag ccttcctctc
      781 gtagtccgct gctttactta tttacacctt ttcatacaga aatgcagcta ctttttttag
      841 cccgtattgt tcaggtttgt agacagactc cgtagcgcct ataaagaaca atcccccagg
      901 cactagcgaa tccgcgatgt tttggtatat cttttctttt acttcattct tgaaatagat
      961 tgtgacgttt ctgcagacaa tggtatggta gcccttttcg taggagtcca gaatcagatc
     1021 atgttttttg aaatgaaccg tttttttgat gtcatccgac aacacatacc ggttgttctg
     1081 ctcggtaaaa tagagtcttt tttccatggg gggaacgttc tgcacttcca atttggagta
     1141 cgttccagcc ttagcttttg acaaaatggt ttcgtctata tctgtggcga ttatcttgct
     1201 gttcgcagtg agcatatttt ttttcaggat catagcgatc gaataagcct ccgctcccgt
     1261 cgaacaggct gcactccaca ctttcaaggc tttgaacttc gaggaaagtc tggtgatgaa
     1321 tacttcttca aattcctcaa atatttcctt gttgcgatag aattccgtca cgttgattgt
     1381 gatgtaatcc aaaaaactct ttttaatgtt atcatcggtt tcgatcattt tggcaaattg
     1441 cttcaaatcg gttgctccgg attttttcat gatggtctca atccgtcttt gtaattgccc
     1501 ttctttgtat gctatcaaat tcaagtgcaa cgttttttct gaccaagcat aaaaccagtt
     1561 aaagtccaac atttatttcc tcccgcctag tacttggttt agcgcatctg tcaattcttc
     1621 cagattgact acttcgtcga tcaccgtatt ctggatggca ttgccgagca tgccaaaacc
     1681 caaggaagat tctttgtcct gagcgaaggt atatcctcca ttagtcttta ttttctttag
     1741 gccttccgta ccgtctttac ccataccggt aagcacaacc ccgatcaggc ggctaccgta
     1801 actttcggct gctgagctga aaagatgggc cacagctggc ctcacaccat gaattttatc
     1861 ggcttgagac agttggatgc ggcgattttt caccgtcata tg
//
LOCUS       DAOMUJ010000369         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17156_length_1898_cov_2.108519, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000369 DAOMUJ010000000
VERSION     DAOMUJ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17156_length_1898_cov_2.108519"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1150)
                     /gene="holA"
                     /locus_tag="PLT73_08315"
     CDS             complement(116..1150)
                     /gene="holA"
                     /locus_tag="PLT73_08315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRM20413.1"
                     /translation="MNYTTEMSKIKKGQLQPVYLFLGKEDFFIEEAKQLLLRTVVDEA
                     DKDLNVGIFNMDGTLLDRALEDAESLPFFGERRLVIIENPLFLTAEKSKNGLEHDLGW
                     LESYLENPAPSTVLAIFAPYEKLDSRKKIAKLLTKKAVTVDVSPLAEKEARKFLGDMI
                     KNEGYQMNRDALELFYERIENQLSRGMRELPKLFLSGSEEKKITKQMVIDLIPRNLEQ
                     NIFELVTQVLNKNTYLAIQIYRDLLLQKEEPIKVNAILLGQFRLLLQVKLLSKNGYQQ
                     TDITKVLKVHPYRVKLAVQQVRHLSEKVLADAFQGLVETEYNMKTGQGLKEIQFELFL
                     IRYANAMPKQ"
     gene            complement(1173..>1898)
                     /locus_tag="PLT73_08320"
     CDS             complement(1173..>1898)
                     /locus_tag="PLT73_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRM20414.1"
                     /translation="GMLTFEKETWQVRETATTAGEKLVSIIKAEGVKKLDRVFLTHAD
                     QDHVGSLRELAEGLPIDAVYFPKGAEGNAAFAAVLLELQSQHQVGLFPLLGKVDLEID
                     ADFVFHILAPLAPGEGGNEDSLVIQTSIGGLDWLFTGDLGEEGEEVLMRTYPDLKTDV
                     LKIGHHGSATSSSEGFLDHVQPELALISAGKQNRYGHPDAQILERLELRNIPIFRTDR
                     QGAIHFLYGSEETEWRTILENKK"
BASE COUNT          477 a          485 c          371 g          565 t
ORIGIN      
        1 tctcgtttaa ctaaaaaatt agcaaaataa ttttttgatt taagagactt caaaacgtaa
       61 ggccgaagac agacccgctt agctggtccg tcttcggcct ttttcgtatg atttttcact
      121 gcttgggcat tgcgtttgcg tagcggatca gaaataactc aaattgtatt tccttcagcc
      181 cttgccctgt cttcatgttg tattccgtct cgaccaatcc ctgaaaagca tccgccagga
      241 ctttttcgga taggtgcctc acttgttgga cagcaagttt cacacgataa ggatggacct
      301 tcaatacttt ggtgatgtcg gtttgttggt acccattttt tgaaagcagc ttcacttgca
      361 gcaacaaccg gaattgccct aaaaggatag cattgacttt gataggttct tctttttgga
      421 gcaatagatc ccgatagatc tggatagcca gatacgtgtt cttgttcaga acctgtgtca
      481 ccaattcaaa tatattctgt tccagatttc gaggaatcaa gtcgatcacc atttgtttgg
      541 tgattttttt ttcttcagat ccagaaagaa atagcttggg caactctctc attccccgcg
      601 acagttgatt ttcgatccgc tcgtaaaata attcgagtgc gtcgcggttc atctggtaac
      661 cttcattttt gatcatgtcc cctaaaaatt tacgggcttc tttctccgct aatggagaaa
      721 catccacagt gacagccttc ttcgtcaaaa gttttgctat tttttttctg ctgtccaatt
      781 tttcgtaagg cgcaaaaata gccagaaccg ttgaaggcgc cggattctcc agatagctct
      841 ccagccaacc aagatcatgt tccagtccat ttttggattt ttcggccgtc aaaaagagcg
      901 gattctcgat aatgaccaaa cgtctttccc caaaaaaagg cagcgactcc gcatcttcaa
      961 gagcccgatc caacaacgtc ccatccatat tgaatatgcc gacattcaaa tccttgtcgg
     1021 cttcgtcaac caccgtacgc agcaacagct gcttcgcttc ttctataaaa aaatcttctt
     1081 tgcccaaaaa taaatagacc ggctgaagct gacctttttt tattttggac atctccgttg
     1141 tataattcac gtttggacac cattcatcat ttttattttt tgttctccaa aatggttcgc
     1201 cattctgttt cttctgaccc atacaaaaag tggatagcgc cctgtcgatc cgtacgaaat
     1261 atagggatat tccgcaattc cagacgttcc agtatttgcg catccggatg tccataccgg
     1321 ttctgttttc cagccgatat caaggccaat tcaggttgga catgatccaa gaaaccttcc
     1381 gaactggatg ttgcgctacc gtgatgacct attttcagga catcggtctt cagatcagga
     1441 taagtccgca tcagcacctc ttcaccttct tctccgagat cgcctgtgaa caaccaatcc
     1501 aaaccgccta tgctggtttg gatgaccaat gaatcctcat ttccgccttc acccggcgct
     1561 aaaggtgcca ggatgtgaaa aacaaaatcg gcatcaatct ctagatcgac cttgcccaat
     1621 aaagggaaga gacccacttg atgctggctt tgcagttcaa ggagcactgc ggcgaaagcc
     1681 gcattcccct ccgctccttt agggaaataa accgcatcga tcggcaaccc ttcggctagt
     1741 tcccgcaacg aaccgacatg atcctgatcg gcatgtgtca gaaagaccct gtccaatttt
     1801 ttcacgcctt ccgcttttat gatcgacaca agcttttcgc cggcggtggt cgctgtttct
     1861 cggacttgcc aggtttcttt ttcaaaagtc agcatgcc
//
LOCUS       DAOMUJ010000370         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17181_length_1896_cov_2.303639, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000370 DAOMUJ010000000
VERSION     DAOMUJ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17181_length_1896_cov_2.303639"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLT73_08325"
     CDS             <1..434
                     /locus_tag="PLT73_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM20415.1"
                     /translation="EVSVQDWYLAGHSLGGVMASSFAADQTDRVDGLILLASYPSNDL
                     TDAPFSALSIYGSEDEVLSRESYDEAQEKLPDDYTEIVAVIMANSAITAYRKAMVRQP
                     SVPFNSNSRRSRPSQHSSETATSNNAADQTVREICMKGRQI"
     gene            431..1042
                     /locus_tag="PLT73_08330"
     CDS             431..1042
                     /locus_tag="PLT73_08330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM20416.1"
                     /translation="MTEITMAKMSQLSETQKEEVREIFVTSYYKDMKTLHRDTERLLG
                     GFRRLLQEDLIRVAMDGERPVAMVGCSTNQRRAMEVNKEDFLANFGFLWGHVGYFSFR
                     KEYGEPLDIDDDTLYFECVATNEADRCQGVAGRMLLKLIETEPYETFALDVVDSNGRA
                     QSVYERIGFREVHRKKSWIGKIFMDYSESIWMSRPKHLPNPSE"
     misc_binding    1107..1376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1498..>1896
                     /locus_tag="PLT73_08335"
     CDS             1498..>1896
                     /locus_tag="PLT73_08335"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746057.1"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM20417.1"
                     /translation="MDYKKIVAEEIQKLVSEHLTYDQVYQLLEVPKYTEHGDVAFPAF
                     ALAKALRKAPQAIAGDLAAQLNHPYVEKVEAVGPYVNLFLEKAAVSNAVLHEIAAEKQ
                     AFGTADIGKGGNVPIDMSSPNIAKPISMGHL"
BASE COUNT          537 a          405 c          522 g          432 t
ORIGIN      
        1 aggaagttag tgtgcaggat tggtatcttg ccggtcattc tttgggcggt gtcatggcct
       61 ccagttttgc ggccgatcaa actgatagag tcgacggcct gattttactg gcatcctatc
      121 cgtccaatga tttgactgac gcgccgtttt ctgccctctc catttatggt tcggaggatg
      181 aagttttgag ccgtgaaagc tacgatgaag cgcaagagaa gttgccggat gactatacgg
      241 aaatcgtggc ggtaatcatg gccaattcgg cgattacggc ctacaggaag gcgatggtac
      301 ggcaaccatc agtgcccttc aacagcaaca gcagacggtc gaggccatca cagcattcat
      361 cggaaacagc caccagcaat aatgcagcgg atcaaacagt acgtgagatt tgcatgaagg
      421 gaagacaaat atgacagaaa taacgatggc gaaaatgagc caattatcgg aaacacaaaa
      481 agaggaagtc agagaaattt tcgtcaccag ctactataag gacatgaaga cgctgcatcg
      541 cgataccgag cgcttgcttg gcggattccg cagactgttg caggaggacc tgatccgagt
      601 tgcgatggac ggggagcgcc cggtcgcgat ggtgggctgt tcaaccaacc agcgtcgtgc
      661 gatggaagtc aacaaagagg atttcctggc taatttcgga tttctctggg gacatgtcgg
      721 ttatttcagt tttcgcaaag aatacgggga gccattggac atcgacgatg atacgctcta
      781 ttttgaatgt gtagctacca acgaggcaga ccggtgccaa ggtgttgccg gacggatgct
      841 gcttaagctg atcgaaacgg aaccttacga aaccttcgcc ttggatgtcg tcgacagtaa
      901 cggacgggct caaagtgttt acgaaaggat tggcttccgc gaagtgcatc gcaaaaaatc
      961 ctggatcgga aaaattttca tggattactc ggaaagcatc tggatgagcc gtccgaagca
     1021 tttgccgaat ccatcggaat gatgaggaag tcaaaaattc aattagacaa acagggccgg
     1081 ctttgatata gtaatacaga tagtaatcac attcattgat gaagaaagta cttcgagcaa
     1141 tgttcacagc gagcggggaa ggtggaagcc cgcaacggag tgaagaagga aacgcacttc
     1201 agagaaaatg acttgaaaac aggcgcggtc ctctgttcaa cggacggatc gggaaagagt
     1261 ctgcgagtag cgtcgttcgg tttagccccg ttaacggttc aaaaggataa actaatcaga
     1321 gcccatctgt gttgctttat cgaattagag tggtaccacg gatgttcgtc tctattttgc
     1381 cagggcagaa taggggcttt ttttatcgtt atcgcagatt tgcggtacct actggaagat
     1441 aagtgatcca ttcaccgtgt ggataggcag aggcatataa taaaggagca gaaaaatatg
     1501 gactataaaa agattgttgc tgaagagatt caaaagctgg tatcggagca tttaacttac
     1561 gaccaagtgt accaattgct ggaagtacct aaatatacag agcacgggga cgtagcattt
     1621 ccggcattcg cactggcgaa agcattgcgc aaagcgccgc aagctatcgc cggagatttg
     1681 gcggcacaac tgaaccatcc ttatgttgaa aaggttgagg cggtcggccc gtacgtcaat
     1741 ctgttccttg aaaaagcagc ggtatcgaat gcggtcttgc atgaaattgc tgccgagaag
     1801 caagcgttcg gaacagccga tatcggaaaa ggcggcaacg tgccgatcga tatgtcctcg
     1861 ccgaatatcg cgaaaccgat ctcgatgggc catttg
//
LOCUS       DAOMUJ010000371         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17185_length_1896_cov_1.751222, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000371 DAOMUJ010000000
VERSION     DAOMUJ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17185_length_1896_cov_1.751222"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLT73_08340"
     CDS             complement(<1..549)
                     /locus_tag="PLT73_08340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM20418.1"
                     /translation="MKKGVTFYFMRHGETYLNKYNRMQGWSDTPLTPRGRRDVMRSGA
                     GLGDVKFDAVYCSDLRRTFETAQIILKENQHADHLKVVAMPEFREIFFGSFEGLDASE
                     TWGNLNKFLGSADGEPNYEAHRSVIEELNGLKEMDPYHEAEDYMTFWLRVERGLIKLI
                     SEHRDSERNILIVSHGMTIRNMI"
     gene            complement(649..>1896)
                     /locus_tag="PLT73_08345"
     CDS             complement(649..>1896)
                     /locus_tag="PLT73_08345"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HRM20419.1"
                     /translation="IRICARYNNLEIIEDAYGISLRPLITFAEMVYKEDRYEPFMPKI
                     NTEDESKIFPEEIRQIAKMNQAISIIQFKLEGEIIKRHPEFDMADRMVLDFINYETGT
                     VKLDGKEYPMLCDYFPTVDPADPYRLTEEERLVVERLMTGFLNSERLQKHVQFLFSKG
                     SMYLSYNDNLLFHGCVPLNPDGSFMELPIANVKYSGKALLDKYEEVLRKGYLNREGKK
                     HNVRILDLIWYLWEGKASSLFGKDKMTTFERLYVAEKETHIEKKNLYYEQRDNVELSH
                     KILKEFGLDPNKGHIINGHTPVKEKIGENPLKADGKLIVIDGGFSKAYQNTTGLAGYT
                     LLYNSFGMQLVSHQPFTSRQDAIENEKDIVSTRRIVDKELERKTVRQTDVGKKLTQQV
                     TDLHQLLRAYKSGEIVEDLLSDK"
BASE COUNT          414 a          492 c          417 g          573 t
ORIGIN      
        1 gatcatgttg cggatcgtca tcccgtggga aacaatcagt atgtttcttt cggaatcacg
       61 gtgctcgctg atcagtttga tcaagccgcg ctcgacgcgt agccagaaag tcatataatc
      121 ttccgcttca tgatacggat ccatttcttt caatccgttc agctcttcga tgacggagcg
      181 gtgggcttca taattcggct cgccatccgc gcttccaagg aatttattca ggttccccca
      241 ggtttcgctc gcatccagcc cttcaaaaga gccaaagaat atctccctga actcaggcat
      301 agccactact ttcaaatgat cggcatgctg attttctttc aaaatgatct gtgctgtttc
      361 aaatgtccgt ctcaagtcgc tgcagtagac cgcatcgaac ttgacatcgc ccaagcctgc
      421 tccgcttctc atgacatcgc gtctgcctct gggcgtcagt ggggtatcgg accagccttg
      481 catccgattg tatttattaa gatacgtttc tccgtgtcgc ataaaataaa acgttactcc
      541 ctttttcaag gttaacccgc ctctcttaag ctattttcgc ttttggatac ttgctttcct
      601 tctcttatct taacaattcc ggcggccgat tgcatctatt tcgtgcaact atttgtcgct
      661 gagcaaatct tccacgattt caccggattt ataggcgcgc aacagctggt gcaggtcagt
      721 gacttgctgg gtcagcttct tgccgacatc cgtctgcctt accgtcttgc gttccaattc
      781 cttgtcgacg atgcgtcggg ttgaaacgat gtccttttcg ttctcgatgg catcctgacg
      841 ggaagtgaac ggctgatgcg aaaccagctg catcccaaaa gagttgtaca ataatgtata
      901 accggccaac cctgtcgtgt tctggtaggc tttcgaaaag ccaccgtcga tgacgatcag
      961 ctttccgtcc gccttcagcg gattctcgcc gattttttct ttgaccggtg tgtgcccgtt
     1021 gatgatatgg cctttattgg gatccaatcc gaactccttc agtattttgt gtgatagttc
     1081 cacattgtcg cgttgctcgt aatacaggtt cttcttctcg atatgcgttt ctttttccgc
     1141 gacataaaga cgctcgaaag tcgtcatctt atccttgccg aacaaggacg atgcttttcc
     1201 ttcccataga taccaaatca aatccagaat acgcacatta tgtttctttc cttcgcggtt
     1261 caaatagcct ttgcgcaata cttcttcgta cttgtccagg agggctttgc cgctgtactt
     1321 gacgttcgcg atcggcaact ccatgaagct gccgtccgga ttcagcggca cacacccatg
     1381 gaacaaaaga ttatcattgt agctcaaata catgctgcct ttgctgaaaa ggaattggac
     1441 gtgcttctgc agtctttccg aattcaagaa gccagtcatg agtctttcta caactaatct
     1501 ttcctcttcg gtcaagcggt agggatcagc tggatcgact gtcggaaaat aatcgcacag
     1561 catcggatat tctttcccat ccaatttcac cgttcctgtt tcgtaattga tgaagtccag
     1621 caccatccga tcggccatat cgaattccgg atggcgtttg atgatttcac cttcgagttt
     1681 gaactggatg attgaaatgg cctggttcat tttggcgatc tggcgaattt cctccgggaa
     1741 gattttgctt tcgtcttcgg tattgatttt cggcatgaac ggttcgtagc ggtcctcctt
     1801 gtagaccatt tccgcaaacg tgatcagcgg acgcaaagaa attccgtagg catcctcgat
     1861 gatctcaagg ttgttgtagc gggcacagat gcggat
//
LOCUS       DAOMUJ010000372         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17247_length_1889_cov_2.004362, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000372 DAOMUJ010000000
VERSION     DAOMUJ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17247_length_1889_cov_2.004362"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>565
                     /locus_tag="PLT73_08350"
     CDS             <1..>565
                     /locus_tag="PLT73_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM20420.1"
                     /translation="NIAESNDEKIEMAQIILDETTEMNKMVNNLLDLSRMQAGYVDLN
                     RQNVDLADFFDHLASRFENSFKEAAIQFTYELADGIKTYAFDEEKMNQVFYNLIDNAV
                     RYSAEIGKRRQKFIHITIRMDDVLDNVIFEVSDNGIGIQAESLPFIFERFYKNDKSRT
                     YSKQKGTGIGLSIVKTIVEAHDGEIEVH"
     assembly_gap    566..575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      756..883
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1007..>1558
                     /locus_tag="PLT73_08355"
     CDS             1007..>1558
                     /locus_tag="PLT73_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM20421.1"
                     /translation="MQQNKTKRLVGISLFGALAFVLMFIAFPVIPAFSYLKIDFSDLP
                     ILLSFFLYGPAGGIISAVIRSILHYLQTGGDMGYPIGDFASLLATLSYTLPVYYLLNK
                     AKTKTAVIMALLTGTLVLTVVLSIANWFIITPLYMYLLNFEMGSVKELVLAGILPFNI
                     IKGLVVSAVILLVMPKLKPWLAHN"
     assembly_gap    1559..1575
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1702..>1889)
                     /locus_tag="PLT73_08360"
     CDS             complement(1702..>1889)
                     /locus_tag="PLT73_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRM20422.1"
                     /translation="QIISPDLFDYTSEGIAFTKMDANAGNAPIPAERFEAFLAAYRKC
                     PTGAILRSESPFPATEQ"
BASE COUNT          526 a          370 c          434 g          532 t
ORIGIN      
        1 aatatcgctg aaagcaatga cgaaaagata gaaatggctc aaatcatttt ggatgaaacg
       61 accgaaatga acaagatggt gaacaatctt ctggatctat cacgtatgca agcgggttat
      121 gttgatctga acaggcagaa tgtcgatctc gcggatttct tcgatcattt ggcaagtagg
      181 ttcgagaaca gttttaaaga agccgctatc caattcactt atgagcttgc agatggcatc
      241 aaaacttatg ctttcgacga agagaaaatg aatcaagtat tttacaatct gatcgataat
      301 gcggtacgat attcggccga aatcggcaaa cgcaggcaaa aattcataca tatcacaatc
      361 cgcatggatg atgtgttgga caacgtcatc tttgaagtca gcgataacgg gatcggtatc
      421 caagcggaaa gtctgccgtt cattttcgag cgattttaca aaaatgataa atctcggaca
      481 tattcgaagc aaaaagggac tggcatcgga ctttcaatcg tgaaaacaat tgtggaagcg
      541 catgatggag aaattgaggt ccatannnnn nnnnnagtga gcccggtgta gggacgactt
      601 tcttggtgcg gcttccattc cacgctgttt gagaagagtg gccggataat actctcaaaa
      661 aaaagctctc aaaaaaaaca tctcgaaacc gtaataacgt gcttgacaag cgctcttgca
      721 tcgcttatag tttatgtata acaaatataa atatgattcg tcttcagggc agggtgaaat
      781 tcccgaccgg tggtgaaagt ccacgacctc gcagtgttac attgcgggct gaactggtgt
      841 aattccagta ccgacagtac agtctggatg ggagaagatg gggtttcttt gaattgcaat
      901 ttcaaaggct ctatttttat gtgtccattt gaggatgata agaatagggt cttttctatt
      961 tggagaaacc catctctgag acagtcaaca gggaggattc attaacatgc aacaaaacaa
     1021 aacaaaaaga ttggtcggta tttcactatt tggggcattg gcatttgtgc tgatgttcat
     1081 cgcttttccg gtaattccag cattttcgta tctgaagatc gacttcagcg acttaccgat
     1141 tctgctttca tttttccttt acggtccagc cggcggaatc atctccgctg tcatccgatc
     1201 tattttgcac taccttcaaa caggcgggga tatgggctat ccaatcgggg actttgcaag
     1261 tctgttggcg acgctctctt acactttgcc tgtctactac ctattgaata aagctaaaac
     1321 aaaaacagcc gttattatgg cgctattgac aggcaccttg gtgttgacgg ttgtcttatc
     1381 aatagccaat tggttcatca tcactccttt atatatgtat cttttaaact ttgagatggg
     1441 atccgtcaaa gaattagtat tggctggtat tttgccgttc aatatcatca aaggacttgt
     1501 cgtaagtgcg gtcatccttt tggtgatgcc gaagttgaaa ccttggttgg cgcataatnn
     1561 nnnnnnnnnn nnnnngtttg cccagtaaga atttgcggtt tatgattttg cttttccaag
     1621 aaaaagaggc tgcatccgaa tcagtgatga ttccgggtgc agcctctttt cgaatatccc
     1681 tgcagcaaag agagttcaga atcactgttc tgttgcaggg aaaggtgatt ctgaacgcaa
     1741 gatggcaccg gttgggcatt tgcgataggc agccagaaac gcttcgaagc gttcggcagg
     1801 tatcggagca tttcccgcat tcgcatccat tttcgtaaaa gctatccctt cggaagtgta
     1861 gtcgaagaga tccggtgaaa ttatttggc
//
LOCUS       DAOMUJ010000373         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17266_length_1888_cov_1.783415, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000373 DAOMUJ010000000
VERSION     DAOMUJ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17266_length_1888_cov_1.783415"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLT73_08365"
     CDS             <1..492
                     /locus_tag="PLT73_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRM20423.1"
                     /translation="NFIVIVFFAAQMMAFFAWSNLGTVIAVKGAELLQNANGIVLIVG
                     FILLTSFINLFIGSASSKWALLAPIFVPMFMLLDFHPAFTQMAYRIGDSITNSLTPMI
                     AYMPLLLATIQRYDKKAGIGTLMANTLPYSIGIGIVWTIILIGWYLLGLPLGPDSPVF
                     LQG"
     gene            517..1716
                     /locus_tag="PLT73_08370"
     CDS             517..1716
                     /locus_tag="PLT73_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM20424.1"
                     /translation="MENLLKKEWMDDLAARKEQVIAYRRHLHQHPELSFQEYETSQFI
                     YDEMSKLENVEVTRPTATSVLVKITGGKPGKKIGLRADIDALPIQEQRDELDFKSVVD
                     GKMHACGHDGHTAVLMVATQFLAEHVNELAGEVYSIFQHAEELPPGGAKEMVATGLFD
                     DFDFIYGHHLFSTMPLGMIDIKPGPNTGNSDLYELTIYGKGGHAAFPHTSIDPIIIGS
                     QILAKIQTIISRKMDPMHAGVISNTVFQAGSPNALNVIPDTAFLAGSVRTNDKGDRLM
                     IAEEIDRVAKATCDAYGATYKLDYLYGYSAVYNNEATTQIVQDIAEELFPGKITHLPP
                     MLGGEDFSAFSDIVPATYIWIGAGNAEKGYDYPHHHPKFALDEESFLIGVKMFVAVAM
                     NYSALAE"
BASE COUNT          433 a          492 c          524 g          439 t
ORIGIN      
        1 aatttcatcg tcatcgtctt tttcgccgct cagatgatgg ccttcttcgc ctggagcaac
       61 ctaggaaccg tcatcgccgt caaaggcgcg gaactgctgc agaacgcgaa cggcattgtg
      121 ctgattgtcg gcttcatcct gttgacttcg ttcatcaacc tgtttatcgg cagcgcaagt
      181 tccaaatggg cattgttggc gccgattttt gtgccgatgt tcatgctatt ggatttccat
      241 ccggctttta cccagatggc ttaccgaatc ggcgactcca tcaccaattc attgacgccg
      301 atgatcgcct atatgccgct tttgttggcg acgattcaac gctatgataa aaaagccgga
      361 atcgggacct tgatggccaa caccttgccg tactcaatcg gcatcggcat cgtctggacg
      421 atcatcctga tcggttggta cttgttgggc ttgccgttgg gaccagacag tccggtattt
      481 ttgcaaggat aagtttcaat cagaaaggag atcactatgg aaaacctatt gaagaaagaa
      541 tggatggatg accttgctgc cagaaaagag caggtcatcg cctatcgccg tcatctgcat
      601 cagcatccgg aattgtcgtt ccaggagtac gagacgagcc aattcatcta tgacgaaatg
      661 tcgaagctgg agaatgtcga ggtgacccgc ccgaccgcga cttccgtgtt ggtgaaaata
      721 accgggggca agcccggcaa gaaaatcggc ctgcgcgccg acatcgatgc cttgcccatc
      781 caggagcagc gggacgaact tgatttcaag tctgtggtgg acggcaagat gcatgcgtgc
      841 ggacatgacg gccacactgc ggttttgatg gttgccaccc agtttttggc cgaacatgtc
      901 aatgaattgg ctggcgaggt ctacagcatt ttccagcatg cggaagaact gcctcccggc
      961 ggcgccaagg agatggtcgc caccggactg ttcgatgatt tcgatttcat ctatgggcac
     1021 catttgttct caacgatgcc gctgggaatg atcgacatca agccgggccc gaacaccggc
     1081 aattccgatc tgtacgaatt gacgatttat gggaaagggg gtcatgcagc ctttccgcat
     1141 acaagcatcg atccgatcat catcggcagt cagattctcg ctaagatcca gacgatcatt
     1201 tcccggaaaa tggatccgat gcatgcgggt gtcatctcga atacggtttt ccaagcggga
     1261 tctccgaacg ccctgaatgt cattcccgac acagcgttcc tggccggcag cgtgcgcact
     1321 aacgataagg gcgaccggct gatgatcgcg gaagagatcg accgtgtcgc caaggccact
     1381 tgcgatgcct atggtgcgac gtataagctg gactacctgt acggctacag cgcggtgtac
     1441 aacaatgaag cgacgacgca gatcgtccag gatatcgccg aggaactgtt ccccggaaag
     1501 atcacgcact tgccgccgat gctcggcggc gaggatttct ccgcattctc cgacatcgtg
     1561 ccggccacct atatttggat cggcgccgga aacgccgaaa aaggctacga ctacccgcat
     1621 caccatccga agttcgcgct ggatgaggag agttttctta tcggggtgaa gatgtttgtt
     1681 gcggtggcga tgaactatag tgcgttggct gagtgagatg accttctggg gccgccagct
     1741 ccgatgagca gggtgctcat cggaagacaa tactgcataa aatagctcac ctccgactag
     1801 cgaatgctgg ccggaggtga gctatttttc ggcggccgaa ctccggcgaa gccgaagtgg
     1861 tcggaggtga tctttttgga tgattatc
//
LOCUS       DAOMUJ010000374         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17293_length_1885_cov_1.791257, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000374 DAOMUJ010000000
VERSION     DAOMUJ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17293_length_1885_cov_1.791257"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLT73_08375"
     CDS             <1..887
                     /locus_tag="PLT73_08375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRM20425.1"
                     /translation="MDTQDSWTVLATNIKERYSSLSGWSIEKDFLTVERMETLRKHFP
                     TATFNHNISTALQNMRLIKSEKEITFMKQAGYWADEALKIGAGTLREGITELEVVAEI
                     EYQLKKRGVAEMSFTTMVLFGENAASPHGVPGDTKLKKNQFVLFDLGTMHEGYASDVT
                     RTFFFGEEPSAHQKRIYELVLAAHDEAMAAVHPGIKAGDLDAVARTIITEAGYGRYFN
                     HRLGHGLGQGVHEYPSIMEGNEMILTENMCFSIEPGIYIEGDIGVRVEDCGYVTADGF
                     ESFTNTPTDIGAYLKLMD"
     gene            complement(970..1434)
                     /locus_tag="PLT73_08380"
     CDS             complement(970..1434)
                     /locus_tag="PLT73_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRM20426.1"
                     /translation="MTKNKGGFVLGAIIGGAAAAITALLFAPKPGKELRDDITEEVNN
                     LLDTARDYADIAVEKSNEFMDVAKETTEDIKINLKDTTSSIKTQFSETTKHVGEDLKK
                     AKDDIKEKATDVKEFASVIAEEGKEEAAAVTEEKKVAVEEKVDEIKDSVEDI"
     gene            complement(1475..>1885)
                     /locus_tag="PLT73_08385"
     CDS             complement(1475..>1885)
                     /locus_tag="PLT73_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRM20427.1"
                     /translation="VVFIILALRKVTDILGEVEKVTQEANKSLAVITKDVDHLSIEVE
                     GLLNKANTLVDDLNGKISKTDPLFTAIGDLGVTVSDVNQSTRNLAVSLKGGGKGVKST
                     SGSSTVGKLGRTAMAIARQRQAKKETKDTKFKTY"
BASE COUNT          457 a          446 c          419 g          563 t
ORIGIN      
        1 acatggatac gcaagattct tggacagtac tggcaaccaa tattaaagaa cgttattcat
       61 cgctgtcggg ctggtccatc gaaaaggact ttttgactgt cgagcgaatg gagacgttgc
      121 ggaaacattt cccgacagct actttcaacc acaacataag tacagcactt cagaacatgc
      181 gcctgatcaa atccgaaaaa gaaattacgt tcatgaaaca agcaggctat tgggctgatg
      241 aagcacttaa aataggtgct ggaacgctgc gcgagggcat taccgaactt gaagtcgttg
      301 ccgaaattga atatcagctg aaaaaaagag gcgtagccga aatgagcttc accactatgg
      361 tgttgttcgg cgaaaacgcg gctagccccc atggtgttcc aggcgatacc aagctcaaga
      421 aaaaccagtt tgtgctgttc gatttgggaa ccatgcatga aggttatgca agtgacgtga
      481 cacgcacgtt cttctttggt gaagaaccct ccgcgcacca aaaacgaatc tacgagttgg
      541 tgttggctgc ccatgatgaa gccatggctg cagtccatcc cggaatcaaa gctggtgatt
      601 tggatgctgt agcgcggaca atcattaccg aagcaggcta cggtcgctac ttcaaccatc
      661 gcctcggaca cggattgggt caaggcgtgc atgagtaccc ttctatcatg gaaggcaatg
      721 aaatgatcct tactgagaat atgtgttttt ctattgaacc cggcatttat atcgaagggg
      781 atatcggcgt cagagtcgaa gattgcggtt atgtgactgc ggatggtttt gagtccttca
      841 ctaacactcc aaccgatata ggtgcatatt tgaaattgat ggattagttg attgaatcag
      901 tatagcagca gcgatcagct aaacagtgtg caaaaaaaac aggcacccgg cctgtttttt
      961 tttgcgcatt cagatatctt ccacgctgtc ttttatttca tcgacctttt cttccactgc
     1021 gacctttttt tcttccgtta cggcggccgc ttcctctttg ccttcttcag cgatgacaga
     1081 agcaaattct ttcacatcag tagctttttc tttgatgtcg tccttagctt ttttcaagtc
     1141 ttcaccgaca tgtttcgttg tttcagagaa ttgtgttttg attgaagaag tggtgtcctt
     1201 caagttaatt ttaatatctt cggtggtttc tttggcgacg tccatgaact cattgctctt
     1261 ctccacagcg atatccgcgt aatcgcgcgc tgtgtccaga agattattta cttcctccgt
     1321 gatgtcatcg cgtagttctt tgcctggttt aggcgcaaac agcaaagctg tgatggctgc
     1381 tgcagccccg ccgatgattg cccctaatac aaatccgcct ttattttttg tcataatcct
     1441 caatctcctt tttgtttatt tattggttga cgcttcagta agttttgaac tttgtatcct
     1501 tcgtttcttt cttcgcttgc cgttgccggg caattgccat cgctgtccgc ccaagctttc
     1561 cgaccgtgga ggaacctgat gtgcttttga ccccttttcc gccaccttta agtgataccg
     1621 ccaaatttct ggtagattgg ttcacatcag aaaccgtcac tcccagatcg ccgattgcgg
     1681 tgaataaagg atctgtcttg ctgattttgc cgttcaggtc atccactaac gtattggcct
     1741 tattcagtaa gccttcgact tcaatcgaca agtggtcaac atctttcgtg atgacggcca
     1801 agcttttgtt cgcttcctgg gtcacttttt caacctcgcc taagatgtca gtgactttcc
     1861 ttaatgccaa aatgatgaaa accac
//
LOCUS       DAOMUJ010000375         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17418_length_1875_cov_2.228022, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000375 DAOMUJ010000000
VERSION     DAOMUJ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17418_length_1875_cov_2.228022"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..>1602)
                     /locus_tag="PLT73_08390"
     CDS             complement(153..>1602)
                     /locus_tag="PLT73_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM20428.1"
                     /translation="EVSLSEAAILAGILKSPSYYNPIDNKEASIARRNTVLQLLADNA
                     FIDQATADAAMAEDINVVGTYYANSSYNYPYYFDAVIDEAVNTYGLDEEDLLNRGYKI
                     YTGLDQDYQIQMDNAYANAYLPTAEDGTLVQSASVAMEPATGDVLAVVGATKYEGFRT
                     YNRATQMPMAPGSTIKPLSVYTPALEAGYAVDAEVMDDDSLTYGDDAYSVSNYDRNSL
                     YDTLPMYQAIAESKNTSAVWLLDKLGIQRGISKLEDFGISIGEADENLGAIALGGMSK
                     GVTPVQLASAYTTFVNEGVRTQARFITKIVDATGAVIVDNTKPKTNRVTTAAVAEEMT
                     SMLMGVYADGGTGASAQPSNGMVIAGKTGTTELDRETGYIKSQWMVGYTPDLVVATWL
                     GYDKTNEIYNLNNAGKTMANLYSAEMGALLSISPQTEFAVAAAEETYDSGIINGLKNG
                     LDEFMNDMGSFGQSLGEGSKKLFDWANEAFGQ"
     assembly_gap    1603..1616
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1617..>1875)
                     /locus_tag="PLT73_08395"
     CDS             complement(<1617..>1875)
                     /locus_tag="PLT73_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRM20429.1"
                     /translation="DPIGIGRAAVGFVLNGGNIVGGGSTLTQQLAKNAYLTLDQTLIR
                     KAKELFLAFEIESKYTKDEILEMYLNNAYFGSGVWGVEDASQ"
BASE COUNT          419 a          522 c          426 g          494 t
ORIGIN      
        1 agcggacgtt tttttgtccg tgtgtggtag tcagcttagg cacagatttc tgggattcag
       61 aacgcgttta cgcagagcgt gatgattcgg gggagaggat gagcacaaaa aaagacaagc
      121 cgtcacagcc tgccttcccc ttaaatcaat tcttactgcc cgaaagcctc gttggcccaa
      181 tcgaacaatt tttttgaacc ctcacctagg ctttgaccga acgaacccat gtcattcatg
      241 aactcatcca agccattttt cagcccatta atgatgccac tatcataggt ttcttcggca
      301 gcagcgacgg caaattctgt ctgcgggctg atcgacagca aagcccccat ttcggcactg
      361 tataaatttg ccatcgtctt gccggcattg ttaagattgt atatttcatt ggttttatca
      421 tatcccaacc aggtagcaac aacaagatcc ggtgtgtatc ctaccatcca ttgtgacttg
      481 atgtagcctg tttcccgatc aagctccgtc gttccggtct tgccggcgat caccatgcca
      541 ttcgatggtt gcgccgatgc cccggttccg ccatctgcat acacgcccat cagcatgctt
      601 gtcatttctt ccgcaaccgc ggcggtcgta acccggttcg tcttcggctt ggtattgtca
      661 acgatcaccg ctccggtcgc atcgacaatc ttcgtgatga aacgtgcttg cgtgcgcacc
      721 ccttcattca cgaaagtcgt ataggcactg gccagttgca ccggtgtcac gcccttgctc
      781 atcccaccga gcgcaatagc gcccaaattc tcatccgctt cgccgatcga gattccgaaa
      841 tcttccagtt tggagattcc tctttggata cccaatttat ccaacagcca tactgcagat
      901 gtgttcttgc tctcggcaat cgcctggtac atcggtaaag tgtcatacaa gctgttccgg
      961 tcatagttcg aaacagaata agcatcatca ccgtaagtca aggaatcatc gtccatcact
     1021 tccgcatcga ctgcgtatcc ggcctcaaga gccggtgtgt agacggacaa tggtttgatg
     1081 gtcgaaccag gcgccattgg catctgcgtt gcacggttat aggtccggaa accttcatat
     1141 tttgtcgctc cgacaaccgc gagtacatcc ccggttgccg gttccatagc cacagaggca
     1201 ctctgtacaa gagtcccatc ttccgctgtc ggcagatagg cattcgcata ggcattatcc
     1261 atctgaatct gataatcctg atccagaccg gtgtagattt tgtagccccg gttcagcaga
     1321 tcctcttcat ccaagccgta ggtattgacg gcctcatcga tgaccgcatc gaaatagtag
     1381 ggatagttgt aactgctgtt tgcataatag gtacccacca cattgatgtc ttcagccatt
     1441 gccgcatcag cggtggcttg atcgatgaac gcgttgtccg ccaacagttg cagaaccgtg
     1501 ttccttctag caatgctcgc ttctttattg tcaattggat tgtagtaact cggcgacttc
     1561 aggattccgg ctagaatcgc tgcttccgac aagctgactt cannnnnnnn nnnnnntctg
     1621 cgaggcatcc tcgacacccc acaccccgga accaaagtag gcgttattca ggtacatctc
     1681 aaggatttcg tccttcgtgt atttgctttc gatttcgaac gccaaaaaca gttctttggc
     1741 tttgcggatc agcgtctgat ccagagttag gtaggcattt ttggccagtt gttgggtaag
     1801 cgtcgaaccc ccaccgacga tattaccgcc attcagcacg aaaccgacag cggcacggcc
     1861 gattccgatc gggtc
//
LOCUS       DAOMUJ010000376         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17425_length_1875_cov_1.785714, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000376 DAOMUJ010000000
VERSION     DAOMUJ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_1875_cov_1.785714"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLT73_08400"
     CDS             complement(<1..810)
                     /locus_tag="PLT73_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRM20430.1"
                     /translation="MTKELIVRTGPQEYECREGVLSTLPKRLEERFVKNILIVHGAVS
                     WQKARPYLEALYKTDFSISEVAFSGECSYEEVDRIVRLSSQNDCDAIIGVGGGKIMDA
                     VKYAAAKAGCILSVMIPTLASNCAPWTPLSVMYTEEGVFIRYDFLQQQASLLLLEPRL
                     IIDSPKDFFVAGLADTLAKWYESDEILSLPENAQQPMLMMARQAASICRQSILDHAEL
                     AIASLEAGEVTEAFVKLTEVITSISGMVGGMGDAFARTTIAHEIHDAITNFP"
     gene            complement(834..1295)
                     /locus_tag="PLT73_08405"
     CDS             complement(834..1295)
                     /locus_tag="PLT73_08405"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HRM20431.1"
                     /translation="MAKVESFELDHDLVKAPYVRQAGYEVHQTGAIVTKFDLRFVQPN
                     QDALPTGALHTLEHLLAINIRDYVDGVIDLSPMGCRTGFYMICWGEHTPEEIASALEK
                     VLQIVLETTVVPATTAKECGNYRDHSLFGAQEYAKQVLAAGISRDPFTRTV"
     gene            1413..>1875
                     /locus_tag="PLT73_08410"
     CDS             1413..>1875
                     /locus_tag="PLT73_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM20432.1"
                     /translation="MLGKEKRLNLSSRRRKECRTTLIGIVFQQLLTMFSIMFFGFLLI
                     RSNVINSEGTRQISTILSLFVMPATMITSFNADFDAKRLKLLLWATLAAFLTISIRAA
                     IAHVLLKKEDRIDKYATIFPNAGFVGIPLVLATVGYEGVFFMSGYIMANNVT"
BASE COUNT          461 a          486 c          415 g          513 t
ORIGIN      
        1 ggggaaatta gtgatggcat cgtggatttc atgggcgatt gtcgtacgcg caaaagcgtc
       61 acccatcccc ccgaccattc cgcttatgga agtgatcact tccgtcagtt tcacaaaagc
      121 ttccgtcact tcgcctgctt ccaggctcgc gatcgccaat tcagcgtgat ccaaaatgct
      181 ttgtctgcag atagaagctg cttggcgcgc catcatcagc atcggctgct gtgcattctc
      241 aggcagcgaa aggatttcat ccgattcgta ccatttggcc agcgtatccg ccaatccggc
      301 cacaaagaaa tccttcggcg aatcgatgat caagcgcggt tccaacaaca aaagcgaagc
      361 ttgttgctgc aggaaatcat agcggataaa tacgccttcc tcagtgtaca tgacactcaa
      421 cggcgtccaa ggcgcgcaat tgctcgccaa tgtgggaatc atcacactca ggatgcaacc
      481 cgctttcgcg gcagcgtatt tcacggcatc catgattttt ccgccaccga caccgatgat
      541 tgcatcgcaa tcgttttgtg aagacaagcg gacaatgcga tcgacttctt cataactgca
      601 ttcgcccgaa aaagccactt cagaaatcga aaagtcagtt ttgtagagag cttcaaggta
      661 cgggcgggct ttttgccaag atacggctcc atgcacaatc aaaatatttt ttacgaagcg
      721 ttcttccaaa cgtttcggta aagtcgacag aacaccctct ctgcattcgt attcttgcgg
      781 tcctgttctt acgatcaatt ctttagtcat tgcagattct cctttctcgt tatctagact
      841 gtgcgcgtga aagggtcgcg gctgataccg gctgccaaca cttgcttcgc gtattcttgt
      901 gcgccgaaca aggaatgatc gcggtaattt ccgcattcct ttgcggtcgt tgccggaacg
      961 actgtcgttt ccaatacaat ctgcagcact ttttccaaag cagaagcgat ttcttccgga
     1021 gtatgttcac cccaacagat catgtaaaac ccggtacggc aacccatcgg tgacaaatcg
     1081 atgacgccat ccacataatc acggatattg atcgccaaaa ggtgttccaa ggtgtgcaga
     1141 gcccctgtcg gtaaagcatc ctggttgggt tgcacaaaac gcagatcgaa ttttgtgacg
     1201 atcgctcccg tttggtgcac ttcatagcct gcttggcgga catacggagc cttcaccaaa
     1261 tcgtggtcca attcaaaact ctctacttta gccatttata tttctccatt cctcagctca
     1321 actcgctgtt gtttgtttta cgatgcatac caatacatta tactagacat ttatgagatt
     1381 ttccttaaaa ttatatcacg tggtgtgtta tcatgttagg aaaagaaaaa agactgaacc
     1441 taagttctcg tagacgaaag gagtgccgca ccactttgat aggcatcgtt tttcaacaat
     1501 tgctgaccat gttcagcatc atgttctttg gttttcttct gatccgatca aatgtcatca
     1561 acagcgaggg tacgcgtcag atatcgacca ttctttccct gtttgtgatg cctgcgacta
     1621 tgatcacctc gtttaacgcc gattttgacg caaagcggtt gaagctgttg ttatgggcga
     1681 ctttagcggc ttttctgacg atttccatcc gcgcagccat tgcccatgtg ctgctgaaaa
     1741 aagaggatcg catcgataaa tatgccacaa ttttcccgaa tgctggcttc gtcggtatac
     1801 cattggtact cgcgacggtg ggctatgagg gcgtcttttt catgtccggc tacatcatgg
     1861 caaacaacgt caccc
//
LOCUS       DAOMUJ010000377         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17471_length_1871_cov_1.610683, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000377 DAOMUJ010000000
VERSION     DAOMUJ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17471_length_1871_cov_1.610683"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..234)
                     /locus_tag="PLT73_08415"
     CDS             complement(88..234)
                     /locus_tag="PLT73_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20433.1"
                     /translation="MKLLSNELDATGIVTGDTALAIVEKLREVLTENDTQYDKAVEIL
                     MGNE"
     gene            complement(215..>889)
                     /locus_tag="PLT73_08420"
     CDS             complement(215..>889)
                     /locus_tag="PLT73_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRM20434.1"
                     /translation="DEEYPEIGIVHVSSFSTPTYDDIVSAVTDLRTQGATSFVFDFRQ
                     NPGGLLDQALKISNMFLDDGDVIMQTQEKDAEPNKIVASDSEFGDFKITEPSVLLVDE
                     GSASASEIVAGALQESSEIPLVGTTTFGKGTVQTVYPLTESSELKLTVAKWLTPNGNW
                     IHEKGIAVDYEVALPEYATLTIIDSTATYEEGTVSEEVKNVEAMLNAIGYAVEADGYY
                     DEATVE"
     assembly_gap    890..915
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<916..1487)
                     /locus_tag="PLT73_08425"
     CDS             complement(<916..1487)
                     /locus_tag="PLT73_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRM20435.1"
                     /translation="MGNNGHKLKKRGVKLSFYFLSLLIVAIAAIVGTYAWIGRSVTLP
                     STVEIIQGTNSSTLDIAASERIGAVYQTLLNNYVEGVDKEALIEGALSGMVEAVGDPY
                     SQYLNTEESENLDESISASFEGIGAEIMSLNQQIVIVSPIKGSPAEKAGLLPNDIILS
                     ADGESLQGLTASEAVALIRGEKGSEVVLEIV"
     gene            complement(1509..>1871)
                     /locus_tag="PLT73_08430"
     CDS             complement(1509..>1871)
                     /locus_tag="PLT73_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM20436.1"
                     /translation="SVVSNTFNGQVNFAPICSFELMHNAYLVAKERNLSVHVGNVLSS
                     DRFYNEELNKQKLADYGVLAVEMEAAGLYLLAAKYNRKALALLTISDHILTGEETTAE
                     ERETTFDDMMLVALESIL"
BASE COUNT          443 a          492 c          402 g          508 t
ORIGIN      
        1 gcaacaaatc caaaggcgat acttccaagc aattccggac attacaaaaa ggtttggccc
       61 ttcgaaaaag cagccaaacc tttcggacta ttcgttcccc atcaatattt ccacagcttt
      121 atcatactgt gtatcgttct ccgtaagaac ttcgcgcaat ttttcaacaa tagccaacgc
      181 ggtgtcgccc gttacgattc cggttgcatc cagctcattc gacagtagct tcatcgtagt
      241 aaccgtctgc ttcgaccgcg tatccgatgg cgttcagcat cgcttccaca ttcttgacct
      301 cttccgagac agttccttct tcataggttg ccgtagaatc gataatcgtt aaagtggcgt
      361 attccggaag agcaacttcg tagtccacag caatgccttt ttcgtggatc cagttgccgt
      421 ttggcgtcag ccacttggcg accgtcaatt tcaattcgct gctctccgtc aaaggataaa
      481 cagtttgaac tgtccctttg ccgaaggtcg ttgtcccgac caacggaatt tcggatgatt
      541 cctgcagagc tcccgcgacg atttccgatg cactggcact tccctcatca acaagcagta
      601 cgcttggttc ggttatcttg aaatctccga actcgctatc actggccaca atcttattcg
      661 gctcggcatc tttttcttgc gtctgcatga tgacgtcgcc gtcgtcaagg aacatattgc
      721 tgatcttcag agcttgatcc aacaaaccgc ctggattctg cctgaaatca aacacaaagg
      781 atgtggcgcc ttgtgtacgc agatccgtta cagcgctgac gatatcgtca taggtcggcg
      841 tcgagaaact ggaaacgtgg acgatgccga tttccggata ttcttcatcn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnacgat ttccaggacc acttcacttc ctttttcgcc tctgatcagc
      961 gcgactgctt cgcttgccgt taagccctgc aatgattcgc catccgcact gagaatgatg
     1021 tcattcggca acagtcctgc tttttcagct ggagagccct tgatcggcga aacgatgaca
     1081 atctgctgat tcaaggacat aatttcagct ccgattcctt cgaatgaggc ggaaatcgac
     1141 tcatccaagt tctcgctctc ttctgtgttc aaatattgac tgtagggatc gccgactgct
     1201 tccaccatgc cgctcaaggc accctcaatc aacgcctctt tatcgacacc ttcgacataa
     1261 ttattgagca gtgtctgata gacagctccg atgcgttcgg atgcagctat atccaatgtc
     1321 gaactgtttg tgccttggat aatctctact gtcgatggga gagtaacgct cctgccgatc
     1381 cacgcatacg tcccgacgat agccgcaatt gcgacgataa gtaaggacag gaagtagaag
     1441 gagagcttga ctccacgttt ttttagttta tgaccgttgt tgcccattca aagtacacct
     1501 ctttaagatt ataggataga ttctaaagca acgagcatca tgtcatcgaa cgtagtttca
     1561 cgctcttcgg cagtcgtctc ttcccctgtc aggatatggt cgctgatggt caataaagct
     1621 aaagctttcc ggttgtattt ggcggccagc aagtagaggc cggcagcttc catttccacg
     1681 gccagaacgc catagtcagc caacttttgt ttattcaact cttcattgta gaagcggtcc
     1741 gaagacagga cattgccgac gtggacggat agatttcttt ccttcgcaac caggtaggca
     1801 ttgtgcatca attcaaagct acagattggc gcaaaattga cttgaccatt gaatgtattg
     1861 ctgacaacgc t
//
LOCUS       DAOMUJ010000378         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17504_length_1869_cov_1.404631, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000378 DAOMUJ010000000
VERSION     DAOMUJ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17504_length_1869_cov_1.404631"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLT73_08435"
     CDS             complement(<1..497)
                     /locus_tag="PLT73_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010624575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRM20437.1"
                     /translation="MDKDRLIAFTDAILAIIMTILVLELKEPETLSLQGLWALKGSYS
                     AYTLSFFWLGTMWIGLHNEWQDVKKISIPVIWYSLSILFFSSLFPYVTKIVSNHFDDS
                     FAQGLYGIIVVLTTLSNILLSDSLVKANAENEVLRRKISFRKRWLTIDLLIKGIGFII
                     AITVYP"
     gene            complement(555..>880)
                     /locus_tag="PLT73_08440"
     CDS             complement(555..>880)
                     /locus_tag="PLT73_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM20438.1"
                     /translation="QAMVAKDIDKLDRILADGMHLVHMTGYDQTKDEWFAQIRSEQMR
                     YYSTKEENIKDIKIEGNKASFIGQSRVDARIYGSRHTWRLQVKNYFEKRNGEWIIVRQ
                     EASTS"
     assembly_gap    881..929
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1399..1815)
                     /locus_tag="PLT73_08445"
     CDS             complement(1399..1815)
                     /locus_tag="PLT73_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007125005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRM20439.1"
                     /translation="MLNQITGKIVPVSSTIKAPFQTDFGRHIFVGENVFINRDCMFVD
                     LGGIYIEDDVLIGPRVTLVSVNHVEDPKQRRDLLPKAVLIKSNAWIGAGATILPGVTV
                     GKNAIVGAGSVVTKNVPDNAVVAGVPAKYIRDIHID"
BASE COUNT          519 a          417 c          344 g          540 t
ORIGIN      
        1 ggatagacgg taatggcgat aatgaagcca atgcctttta tcagcagatc gatggtcagc
       61 caccttttcc ggaaggaaat ttttctccgg agcacctcat tttcagcatt cgccttcacc
      121 agcgaatcgc tcaataagat attgcttaaa gtggtcagca caacgatgat gccatacagc
      181 ccctgagcaa aggaatcatc gaaatgattg ctgacaatct tcgtcacata aggaaagagc
      241 gatgagaaga agaggatgga caggctgtac caaatgaccg gtatggatat tttttttacg
      301 tcctgccatt cattgtgcaa gccgatccac atcgtcccaa gccaaaagaa ggaaagtgta
      361 taggccgaat aactgccttt cagcgcccac aatccttgca aactgagggt ctccggttcc
      421 ttcaactcca aaaccagaat agtcatgatg atagccagaa ttgcgtccgt aaaagcgatc
      481 aatctgtcct tatccatatt catttcccct tttgataatc ttggtagatc aaatctgctg
      541 aaacttagca agctttaaga cgtgctggcc tcttgccgaa caataatcca ttctccgttc
      601 cgcttttcaa aataattttt gacttgaagt ctccaagtgt gccttgagcc atagatgcgc
      661 gcatccactc tgctttgccc gatgaagctg gctttgttgc cctcgatctt gatgtccttg
      721 atgttctctt ctttggttga ataatagcgc atctgttcgc ttctgatttg tgcaaaccat
      781 tcgtcttttg tctggtcata tcccgtcatg tggaccagat gcatgccgtc cgcaagaata
      841 cgatccagct tgtcaatgtc tttggcaacc atcgcttgat nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aatctttatg attagtgcaa ttatagaact
      961 tggagttgac tccaagtcaa gagtaaacga tcaatgacca tttttgatgt aacgggtcag
     1021 atagtgttgg cgtatgcttc ttggcaaatc tgatcattgt cgggtagctt tcttctctct
     1081 cttcaataag aattataacg cgttttatct aacatcgcat tctctacaac gacacctact
     1141 cgcatttatt tgagggcact tgggtcatcc aaacaagaaa ttcccacttc ctgatctgcg
     1201 aaacacgcaa gttggcataa gggaagactt tatcttaata tctcaggctt actttgttta
     1261 cgcaattaag ttaaaaatca ctagtcacac tgtccagaaa tgtagcattt tctggacagt
     1321 gtttttcttt accaaaaaaa aagcaatcaa gaagattgct ttttgaaata tattaatttt
     1381 cttcgtcata aactgggctc aatcaatatg gatgtctcta atatattttg cgggaactcc
     1441 cgcaactacc gcgttgtcag gaacattctt tgtgacgact gaaccagcac caactatcgc
     1501 attcttgcca acagtaaccc cgggcaaaat agtggcgccc gctccaatcc aagcattact
     1561 tttaataagc acggcttttg gaagcaagtc tcgcctctgt tttgggtctt ccacatgatt
     1621 aacggaaact aatgtgactc gaggtccaat caatacatca tcctcaatgt aaataccacc
     1681 taaatcaaca aacatgcaat cccgatttat aaaaacattt tcaccaacaa aaatatgacg
     1741 accaaaatca gtttgaaatg gtgccttgat agttgatgaa acaggcacaa tttttcctgt
     1801 aatctgattt aacagtgatt tagctgtcgt tcggtcgttg gcagaattaa attctgccag
     1861 caatttatc
//
LOCUS       DAOMUJ010000379         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17507_length_1868_cov_2.389410, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000379 DAOMUJ010000000
VERSION     DAOMUJ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_1868_cov_2.389410"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLT73_08450"
     CDS             complement(<1..526)
                     /locus_tag="PLT73_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRM20440.1"
                     /translation="MEISRMTVKGKGFASASPDAIAISLQLEDIKDTYELAMQHAALA
                     LEQVRETLLPLGFSKKEIRSTHFSIDSAYEHRNDFRGENKRYFLGYRYRNDLKITFGN
                     DSKRLGEILLALSVCEADPEFSVYFFLKDRRAVEQALLESAVRDAKEKSQVLAKASGV
                     MLGKILAIDYDWGEI"
     gene            complement(636..1106)
                     /locus_tag="PLT73_08455"
     CDS             complement(636..1106)
                     /locus_tag="PLT73_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20441.1"
                     /translation="MKFYDDNQKKVRYRFGFYSLLFMTALLFIYISLPIDSLGGISYQ
                     NAIMIIIMVSALFFLVNIVYRNAFFDIYTRSPFWSNVFFLVMAGLQAQRSYQLYHLGM
                     DLPVPINTVEFVMLHGLQVVLHLSIPLTYGVRLFVDRQTRKAKEQNAHETGQSS"
     gene            complement(1109..>1868)
                     /locus_tag="PLT73_08460"
     CDS             complement(1109..>1868)
                     /locus_tag="PLT73_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20442.1"
                     /translation="VNKDKIVSELKSIPGLKGVMVCEDGDNGEGSDILLYYGAEEGFH
                     PELDQAIHRLEEGGLRVGFTLRDIGLVDLAITDCLDGKIAIKYETGHPFGVVNSNMAA
                     EIHYGRILWESVDHPITAEKGKLVMQGTYPETLRQSTIACFMKETTLQLEIVKEAAFE
                     GDIHFASAGFSQAIHSWVQVIHALNRQFLLNGKGGVKRAGRLPISPKQFRLRVNQIYR
                     YFAFNNPKLAYCEIDMLKNEMNVLVKTFSEAKEE"
BASE COUNT          519 a          488 c          376 g          485 t
ORIGIN      
        1 cgatttcgcc ccaatcgtaa tcgatcgcca aaattttccc gagcatgacg ccgctagcct
       61 tagccagcac ttgtgatttt tccttggcat ctcgaacagc actttccagc agagcttgtt
      121 cgactgcccg gcgatccttg aggaaaaaat aaaccgaaaa ttccggatcc gcttcgcaga
      181 ccgacagtgc caagaggatt tcacccaggc gtttgctgtc gtttccgaag gtgatcttta
      241 agtcattccg ataacggtaa cctaagaagt agcgcttatt ctcgccgcgg aaatcgttcc
      301 tgtgctcata cgcgctgtcg atcgaaaagt gtgtggaacg tatttccttt ttagaaaatc
      361 cgagtggcaa caacgtttcc cggacttgct ccaacgccaa agcggcatgt tgcatcgcca
      421 attcgtaggt gtccttgatg tcctccaact gcagcgaaat cgcgattgca tcaggcgacg
      481 cgctggcgaa acctttgcct ttgacagtca ttcttgaaat ttccatttcg gttatcccct
      541 ttttaaatag atcaacttgt cttcattaag aaagactatc acgcagaaag cggttacgca
      601 aaaaaaacag agagtcatga aagaaggccc actgttcaag aagactgtcc agtttcatgg
      661 gcattctgtt ccttcgcctt tctcgtttgc cgatcgacaa agagacgcac gccataagtc
      721 aacggtatcg aaagatgcaa gactacctgc agaccgtgaa gcatcacaaa ttcaaccgta
      781 ttgatcggaa caggcagatc catgcccaga tgatacaact gataagaccg ctgcgcctgc
      841 agtccggcca tcaccaagaa gaaaacattg ctccaaaagg gactgcgcgt atagatgtcg
      901 aaaaaggcat tccgataaac aatgttaacc agaaagaaaa gagcactgac catgatgatg
      961 atcataattg cattttggta tgaaatcccg ccaaggctgt cgatcggcaa tgagatataa
     1021 ataaaaagca acgctgtcat gaacaacaaa ctataaaagc cgaatctgta gcgaaccttc
     1081 ttttgattgt catcgtaaaa tttcacattc attcctcctt agcttctgaa aaagttttta
     1141 ccaacacatt catttcattt ttcagcatat caatttcgca ataggcgagc ttcggattgt
     1201 tgaaggcgaa ataacgatag atttgattca cacgcaatcg gaattgtttc gggctgatcg
     1261 gcaatcggcc cgcgcgcttc acgccgccct tcccgttcaa cagaaattgt cggttcaatg
     1321 catgaatgac ttgaacccac gaatgtatcg cttgggaaaa accagcgctg gcgaaatgga
     1381 tatccccttc aaaagcggct tcctttacaa tttccaactg taacgtcgtt tccttcatga
     1441 aacaagcaat cgttgactga cgcagtgttt ccggatacgt tccttgcatc actagctttc
     1501 ccttttctgc tgtgatcgga tgatccactg attcccaaag aattcgacca taatgaattt
     1561 cagccgccat gttgctgttc acgacaccga aaggatgacc agtctcgtac ttgatcgcaa
     1621 tctttccgtc aaggcaatcc gtgatggcaa gatccacaag tccaatatct cttaaagtaa
     1681 atccaacccg taacccgcct tcttccaatc ggtgaattgc ctgatccaat tcaggatgga
     1741 acccctcttc agcgccatag tacagcaaga tatctgaccc ttcgccattg tccccatcct
     1801 cgcaaaccat gacgcccttc aacccaggaa tgcttttcag ttcgctcaca atcttgtctt
     1861 tgttcact
//
LOCUS       DAOMUJ010000380         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17510_length_1868_cov_1.959735, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000380 DAOMUJ010000000
VERSION     DAOMUJ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17510_length_1868_cov_1.959735"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1415
                     /locus_tag="PLT73_08465"
     CDS             6..1415
                     /locus_tag="PLT73_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM20443.1"
                     /translation="MSAGKGFPEGFFWGGATAANQFEGGWDADGKGPSVIDQLTCSRD
                     FAERFAGVGDYHPSHIGIDFYNRYKEDIALFAEMGFRMFRMSIAWTRIFPNGDELEPN
                     EAGWAFYDDVFDELAKYGIEPLVTISHFEMPQYLVDHYGGWKNRKLIGFFERYATTLF
                     ERYQGKVNYWLTFNEINMATFLPQISIGSKAEAGENQEEIMYQALHHQFVASALATKA
                     ARAIDPDNRIGCMIAYAPVYPLSAKPEDVWSAQRKEEEKLFFSDVHARGYYPPSKLAY
                     FATKGINVAFEQGDEEILAAHTVDFISFSYYASSAASFAQSGVPGEGNALMGEKNPYL
                     AESEWGWQVDPLGLRVALNTFHSRYQLPLMVVENGLGAVDEIVDGKIHDDYRISYLRD
                     HVKAMRDAIVLDGVDLVAYTSWGCIDLVSAGTGEMAKRYGYIYVDRNNDGSGSLDRLK
                     KDSFYWYQKAIASNGEDLD"
     gene            complement(1662..>1868)
                     /locus_tag="PLT73_08470"
     CDS             complement(1662..>1868)
                     /locus_tag="PLT73_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076843.1"
                     /note="with PdxST is involved in the biosynthesis of
                     pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of
                     glutamine to glutamate and ammonia; PdxS utilizes the
                     ammonia to synthesize pyridoxal 5'-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRM20444.1"
                     /translation="PFEGIAEPVEAVFIRAPYIRSVGKEVRVLASIDGKIVAAENDQI
                     LVTAFHPELTEDLSVLEYFLKKIG"
BASE COUNT          455 a          465 c          506 g          442 t
ORIGIN      
        1 ttgtcatgtc tgcaggtaaa ggatttccgg aaggtttctt ctggggcggc gctactgccg
       61 ccaaccaatt tgaaggaggt tgggacgctg atgggaaagg tccatcggtc atcgaccaac
      121 tgacctgctc gcgtgacttc gctgagcgct tcgccggagt gggcgattat cacccttcac
      181 acatcggtat cgatttttat aaccgctaca aggaagatat cgctttgttc gcagaaatgg
      241 gcttccggat gttccggatg tcgatcgctt ggacgcggat ttttccgaat ggagatgaac
      301 tggaaccaaa cgaagcgggc tgggcctttt acgatgacgt cttcgatgaa ttggcgaaat
      361 acggcatcga accattagtg acgatctcgc atttcgaaat gccgcaatat ctggtcgacc
      421 actacggcgg ctggaaaaac cgcaagctga tcgggttttt tgaaagatat gcgactacct
      481 tgttcgaacg ctatcaaggg aaagtcaatt attggctgac cttcaacgaa atcaacatgg
      541 cgacgttcct gccgcaaatt tcaatcggtt cgaaagccga agcgggcgaa aaccaggaag
      601 aaatcatgta tcaagccttg catcaccaat ttgtcgccag cgctttggcg acgaaggcgg
      661 cacgggcaat cgatcccgac aaccgcatcg gctgcatgat cgcctatgcg ccggtctatc
      721 cgttgtcggc aaaaccggaa gacgtttggt cagcgcaacg aaaagaagaa gaaaaactgt
      781 tcttctcaga tgtccatgcc cgcgggtatt atccgccgag caaactggct tatttcgcca
      841 ctaaaggcat caacgttgcg ttcgaacaag gcgacgagga aatcttggcc gcacatacag
      901 tcgatttcat ttccttcagc tattatgctt ccagcgcagc cagctttgcg caatccggcg
      961 tgccgggtga aggcaatgcc ttgatgggcg aaaagaaccc atacttggcg gaatcggaat
     1021 ggggttggca agtcgatcca ttggggctgc gcgtcgctct gaacaccttc cattcccgtt
     1081 accaattgcc gctgatggtg gtcgaaaacg gtttgggtgc tgtggatgaa atcgtcgatg
     1141 gcaaaatcca tgacgattac cgcatttcct acctccgcga tcacgtcaaa gcgatgcgtg
     1201 atgcgatcgt gctggacggc gtggatctcg tcgcctatac gtcttggggc tgcatcgacc
     1261 tggtttcggc cgggacgggc gagatggcga aacgctacgg ctacatatat gtggaccgta
     1321 acaacgacgg ttccggcagc ttggatcgcc tgaagaagga ttccttctac tggtaccaaa
     1381 aagccatcgc cagcaatggc gaagatttgg attaaaacat taaaaatccc ctgtaccacg
     1441 caagagcgtg gtggcagggg atttttttag tgctgtccag ccggtagttg tccgataacg
     1501 cttgtttatc ggacaacttt gtgttagtgc tggccgcgat tgtccgatac tttgggggaa
     1561 tcggacaacc tggttccagt aaggggcgaa cttgtctgat accggtaaga aatcggacaa
     1621 gttgggccac gataaggata gaattgtccg ataaacccga cttatccgat tttcttcaaa
     1681 aaatactcca ataccgacaa gtcctccgtc aattccggat ggaaagcagt aaccaaaatc
     1741 tggtcgtttt cggccgctac aatctttccg tcgatgcttg cgagcacgcg gacttccttt
     1801 ccgacggagc ggatgtaagg ggcgcggatg aagacggctt ccaccggctc tgctatccct
     1861 tcaaaagg
//
LOCUS       DAOMUJ010000381         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17639_length_1857_cov_2.293008, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000381 DAOMUJ010000000
VERSION     DAOMUJ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17639_length_1857_cov_2.293008"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1566
                     /gene="acnA"
                     /locus_tag="PLT73_08475"
                     /pseudo
     CDS             1..1566
                     /gene="acnA"
                     /locus_tag="PLT73_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001169.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            1620..>1857
                     /locus_tag="PLT73_08480"
     CDS             1620..>1857
                     /locus_tag="PLT73_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRM20445.1"
                     /translation="MEVHKGLADVVVSETSLSAIVEGQLSYSGYNIDELVEKDASFEE
                     IIFLLWNSRMPSREEFAQLQHDLHTHMALSESVIA"
BASE COUNT          540 a          357 c          480 g          480 t
ORIGIN      
        1 caggtccgaa gcgtccacaa gatttggtac ctgtgtcaaa attgaaaata gatttccaga
       61 gatcattgat tgcgccaaaa ggcaatgccg gatttggact atccgaagag gatgcacaaa
      121 aagaagttac atttgtcctc gatggggaag aagtgaaaat gaagacggga gatgtagcga
      181 ttgctgccat cacaagctgc acgaacacat ctaatccata tgtaatgttg agtgccggat
      241 tattggcgaa acgagcagtc gagttggggt tgaacgtgcc gaaatacgtc aagacttcgt
      301 tggctccagg atccaaagta gttaccgcct atttggataa tgctggttta cttccttacc
      361 tggaagaact gggcttcaat actgtcgggt acggttgtac gacatgtatc ggaaattcag
      421 ggccgctttt gccggaagtg gaagaagcaa tcaaaaacag tgatttgttg gtttctagcg
      481 cactgagcgg aaaccgtaac tttgaagggc ggattcatgc tttggtcaaa gcgaactatt
      541 tggcatcacc gccattggta gtcgcttatg cgctggctgg tacgatgaac atcaatctga
      601 aatccgatcc gatcggaaac ggtaaatttg gctgatctgt ggccggaaag acatactgtg
      661 gaagcgatga tccgagattt tgtaacacct gagatattca aagcagagta tttgcatgtt
      721 tttgatgata atgctgaatg gaacgcaatc gaaacaaatg atgatgcttt gtaccaatgg
      781 gaagagggat cgacttacat tgcaaatccg ccattcttca acaatttatc gcctgaacca
      841 aaagcgattg aatccttgaa ggaattggca gttttgggta aattcggtga ttcggttacg
      901 accgatcata tttctccagc aggaagcatc ggagtcaata caccggcggg gcaatatttg
      961 tcttcgaaag gtttgggagt ccgcgatttc aactcatacg gagctagacg tggaaaccat
     1021 gaagtaatga tgcgtggaac gttagcaaat atccgtatcc gcaatcaatt ggtccctgcc
     1081 aaagaaggcg gtgtcactat ctactggccg acaggtgaag agatgagtat ctttgatgca
     1141 tccgaaaaat accgcgaaga gagtgttggg ctgatcatcc ttgccggtga tgattacgga
     1201 atgggttcat cccgtgattg ggctgccaaa ggtgtcaagt tgctcggggt cgaagcggtc
     1261 atcgcgaaga gctatgaaag aatccaccgt tcgaatcttg tcatgatggg catattgcct
     1321 ttgcaatacc aagcgggtca agatgcagaa agcctcgggc tggacggatc ggaaaaaatt
     1381 acgattgatt tgaacgatag tgtcggtatc catgccgaaa ttcctgtgac ggcagtcaaa
     1441 tcagatggtc aagaaatcaa attcacgact attgcccgtt ttgattctga agtggatatg
     1501 acttactacc gtaacggagg catcctgccg atggtaatcc gtaaaaaaat ggggcttgct
     1561 tattaagcag caagccatcc aatttgctga agagaggttt ttgagaggag agaaaaatca
     1621 tggaagtaca taaaggatta gcagatgttg tcgtatcaga aacctcccta agcgcaattg
     1681 ttgagggaca attatcctat tcaggatata atatcgacga attagtggaa aaagatgcat
     1741 cgtttgagga aatcatcttt ttgttgtgga attcaagaat gcctagccgt gaagaatttg
     1801 cgcagttgca gcacgatctt cacacgcata tggccttatc tgaaagtgtc attgctt
//
LOCUS       DAOMUJ010000382         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17718_length_1851_cov_1.883073, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000382 DAOMUJ010000000
VERSION     DAOMUJ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17718_length_1851_cov_1.883073"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLT73_08485"
     CDS             <1..303
                     /locus_tag="PLT73_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HRM20446.1"
                     /translation="ALAQEAEWLLLDEPFAGIDATSEKIIIELLKQLRDEGKSIVIVH
                     HDLHKVQSYFDDIVLINKEVVAFGTVSEVFTANNMGRAYGEAISEMISLGGGTNVK"
     gene            293..1150
                     /locus_tag="PLT73_08490"
     CDS             293..1150
                     /locus_tag="PLT73_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRM20447.1"
                     /translation="MLNDFIQGLLDFQFLQNALITSVMVGISAGIIGSFIILRGMSLM
                     GDAISHAVLPGVALSYMLGSSYIIGATIFGMASAALIGFVTKHSRLKNDTAIGIVFSA
                     FFALGIILISFARSSTDLYHILFGNVLAVRDSDMFLTAAVLVIVLIAVPLFYKQLKLT
                     SFAPTIAKSYGWNISAINYGLMFLLTLVAVTSLQTVGTILVIAMLITPAATAYQLTDK
                     LLVMIVLSTVFGTLSSIVGLYFSYSYNLPSGATIVMAAAVFFIVAFIFAPKKGLIGQL
                     RKENLQYEE"
     assembly_gap    1249..1270
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1271..>1851
                     /locus_tag="PLT73_08495"
     CDS             <1271..>1851
                     /locus_tag="PLT73_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRM20448.1"
                     /translation="TNSIIGDMVEKVGGDLIDAHSIVPVGTDPHEYEPLPEDIRKASE
                     ADVVFYNGLNLETGNGWFDKLMETAGKAEDEDYFVVSKNVEPLYLSGNSSQQDPHAWL
                     DISNGIRYITEITRVMSEKDPENKTAYEKNADAYIAALTELDSKAKKEFADIPEQKKL
                     LVTSEGAFKYFSQAYGLQAAYIWEINTESQGTP"
BASE COUNT          480 a          389 c          464 g          496 t
ORIGIN      
        1 gcgttggcac aggaagcgga atggttattg cttgacgaac cttttgccgg gatcgatgcc
       61 accagtgaga aaatcatcat tgagctgttg aaacaacttc gcgatgaagg caaatcaatc
      121 gtgatcgtgc atcatgattt gcataaagta cagtcgtatt ttgatgatat tgtcctgatc
      181 aataaagaag tggttgcctt tggtacagtt tccgaggtat ttactgccaa taatatggga
      241 agggcttatg gggaagcgat ttccgaaatg atcagtctag gaggtggaac aaatgttaaa
      301 tgatttcatt caaggactct tggatttcca attcctgcag aacgctttga ttacttccgt
      361 catggtcggg atatctgccg gaatcatcgg ttccttcatc atcctgcggg ggatgtcttt
      421 gatgggggac gccatctcgc acgcagtatt accgggagtg gcgctatctt acatgctggg
      481 gagcagctat atcatcgggg ccacgatttt tgggatggcg tcggccgctc tcattggttt
      541 tgtcacgaaa cacagtcgtt tgaaaaacga tacggcgatc ggaatcgtct tcagcgcctt
      601 ttttgctcta gggatcatct tgatttcctt tgccagaagt tccacggatt tgtatcacat
      661 cctcttcggg aatgtgctgg cagttcggga ttcggatatg tttttgacag ccgctgtgct
      721 tgtcatcgtc ttgatagcag ttccgctatt ctataaacaa ctcaaactga cttcctttgc
      781 gccgacgatt gcaaaatcat acggttggaa catcagtgcc atcaactatg ggttgatgtt
      841 tttgctgaca cttgtggcgg tgacttcgct gcagacagtc gggacgattc tggtgatcgc
      901 aatgctgatc accccagctg cgacagccta tcaactgacc gataaattgc tcgttatgat
      961 tgtcctctcg acagtatttg gaacgctcag ttcgatcgtg gggctttact tcagctacag
     1021 ttacaacttg ccgtccgggg cgacgatcgt catggcagca gctgtatttt tcattgtggc
     1081 atttatattt gcaccgaaaa aaggccttat cggtcaatta agaaaggaga atttacaata
     1141 tgaagaataa actatttctg cttgctgcag tgctttttgt aggtgctttg ggaggttgcg
     1201 gaaattcccc ggatgatgat aaaggcagtg cggatgcaac agacacatnn nnnnnnnnnn
     1261 nnnnnnnnnn ccaccaattc cattattggc gacatggtag aaaaagttgg aggggatttg
     1321 atcgatgcgc acagtatcgt gcctgtcggg accgatccgc acgaatacga acccttgccg
     1381 gaagacattc gaaaagccag cgaagccgat gtcgtgttct acaacggatt gaatttggaa
     1441 accggcaatg gctggtttga caaattgatg gaaactgctg gtaaggcgga agatgaggat
     1501 tacttcgttg tcagtaaaaa tgtcgaaccg ctgtatttgt cgggaaacag ttcgcaacaa
     1561 gatccgcatg cctggctgga tatttcaaat ggtatccggt acatcaccga aatcacgcgc
     1621 gtcatgagcg agaaagatcc tgaaaataaa acagcttatg aaaaaaacgc ggatgcttac
     1681 attgccgctt taacggaact ggacagcaaa gcgaaaaaag aattcgcgga tatccctgag
     1741 caaaagaaac tgttggtgac cagcgaagga gctttcaaat atttctccca agcttacgga
     1801 ctgcaagccg cctatatttg ggaaatcaat accgagagtc aaggcacgcc t
//
LOCUS       DAOMUJ010000383         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17728_length_1850_cov_2.664067, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000383 DAOMUJ010000000
VERSION     DAOMUJ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17728_length_1850_cov_2.664067"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..174
                     /locus_tag="PLT73_08500"
     CDS             22..174
                     /locus_tag="PLT73_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20449.1"
                     /translation="MVLLGPQVRFQKATVEKLVKGRIPVEVMDMRLYGTMNGKAILED
                     ALQKMN"
     gene            193..1557
                     /locus_tag="PLT73_08505"
     CDS             193..1557
                     /locus_tag="PLT73_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20450.1"
                     /translation="MTLMDKFQSIIERLLVPLATKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMDADDLLTGLTAISVYFIIYPDYFNNEGINYITTQYTGAQGLFVAILVGLLV
                     GELMSRLSKSDKLEIKMPEQVPPAVARSFKVLIPIIITTIFFSVLNYFVKMAAPGGLH
                     ELIYSIIQTPLTRMSQSLFSVLVLALLSQSLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LYYADKGTTWGAPYPITYSGLATAFSEYGGSGATLGLIIAMLIFSKNKESKSIAKLSL
                     APGLFNINEMVIFGLPIVLNPIYIIPFIIAPLVNIMIGYTAVMVLKIMPPVTIGVPWT
                     TPGPLMPFLGTGGNITALFVGFICLAVSVLIYSPFVIAANKAAVLEEEAENEISAQEV
                     TGSI"
     gene            1623..>1850
                     /locus_tag="PLT73_08510"
     CDS             1623..>1850
                     /locus_tag="PLT73_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM20451.1"
                     /translation="MDGMELIAFNIISNVGMAKSLAVEALRVAREGNYDEAEQKMAEA
                     SECLVNGHHAHASLIQKEAAGEKVEFSLIIMH"
BASE COUNT          537 a          354 c          414 g          545 t
ORIGIN      
        1 accgctgttg atcatgtcga tattgtcctt ttgggacctc aagtgcgttt ccagaaagca
       61 actgtcgaaa aactcgtaaa aggcagaatt ccagtggaag ttatggacat gcgtctgtat
      121 ggaactatga acggaaaagc gattttagaa gatgctctgc aaaaaatgaa ctaggaaaag
      181 aggatggaaa aaatgactct catggacaaa tttcaaagta ttattgaaag attattagtg
      241 cctctagcta caaaattgaa tgcgcaaaga catatatgtg cagtaagaga tgccttcatt
      301 ttatcattcc cattaacgat ggcgggctct ttgatggtgt tgctgaataa tgtattcttg
      361 tcggcggatg gattcatgtt caaaatcctg caattcgata agctgtttcc tggtattgta
      421 aaattccaag ccgtcttcgc tcctgtcgtt aaaggttccg cagatatttt ctcgatcctg
      481 attgtctttt tgatagccag aaaccttgcg aaactgatgg atgcggatga cctgctgaca
      541 ggtttgacgg caatctccgt ttatttcatc atctaccctg attatttcaa caatgaaggc
      601 atcaattata ttacgacaca atacacgggt gctcaaggct tgttcgtagc gattttggta
      661 gggcttttgg taggggaatt aatgtccaga ttatcaaaat cagataagtt ggaaatcaaa
      721 atgccggaac aagtgccacc ggctgtcgct agatcgttta aagtgctgat accgatcatc
      781 atcacgacaa tcttcttctc tgtcctgaac tacttcgtga aaatggccgc accaggcgga
      841 ttacacgaat tgatttacag catcatccaa acgccattga ccagaatgag tcaaagtctc
      901 ttctctgttc ttgtattggc attgttgtcc caatcgctct gggcaatggg aatccatgga
      961 ccaaatacga ttgcggccat cagagatact atgttttctg aagccggaaa tgcgaatctt
     1021 ttgtactatg cggataaagg gactacatgg ggagcgcctt atccaataac ttatagtggt
     1081 ttagctacgg ctttctctga atacggtggt tcgggtgcga cgttaggtct gattattgct
     1141 atgcttattt tctcaaaaaa taaggaatca aagagtatcg cgaaactttc tttggcacca
     1201 ggattattca atatcaatga aatggtcata tttggattac cgatcgtttt gaatccgatt
     1261 tatatcattc catttatcat agcaccattg gtcaatatca tgatcggcta taccgctgtt
     1321 atggttctta agattatgcc gccagtaacg attggtgttc cttggacaac cccagggcca
     1381 ttaatgccgt tcttaggtac aggcggaaat atcactgcat tgtttgtcgg atttatctgt
     1441 cttgcagtga gtgtgttgat ttactctccg tttgttattg ctgctaataa agctgcagtc
     1501 cttgaagagg aagcagaaaa tgaaatatcg gcacaagaag taaccggatc aatctagacg
     1561 gaatagctgc tgatgcgcta ttcaaaaaat aagctggttg attcagaata ggagatttac
     1621 acatggatgg aatggaattg attgctttca atataatcag taatgtggga atggcaaaat
     1681 cacttgctgt cgaagctttg agagttgcca gggaaggcaa ctacgatgaa gcagaacaaa
     1741 aaatggccga agcatcggaa tgtcttgtga acggacatca cgcacatgcc tccttgatac
     1801 aaaaagaagc tgccggtgaa aaagttgaat tctcgctgat catcatgcat
//
LOCUS       DAOMUJ010000384         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17817_length_1844_cov_1.539407, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000384 DAOMUJ010000000
VERSION     DAOMUJ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17817_length_1844_cov_1.539407"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1262)
                     /locus_tag="PLT73_08515"
     CDS             complement(162..1262)
                     /locus_tag="PLT73_08515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRM20452.1"
                     /translation="MNAITESILKEAADAFAFDRTTGEISRYGEGHINDTFVVWASDK
                     CKRYIMQRINTDTFKNPKELMENVCNVTSFLRKVILENGGDPERETLNVIPTKDRKPF
                     YTDSDGGTWRAYSFVEDTVCLQKVENENDFYTVAETFGTFQNQLAAFPAATLHETIVK
                     FHDTPTRYQNFEKAVAEDAMGRAKDVADEIAFVRSREADCHVLVDMLSKNEIPLRVTH
                     NDTKLNNVLIDKTSKKGICVIDLDTVMPGLAAYDFGDSIRFGANDCAEDEPDQNKVHF
                     DLHLFEVFAKGFLSTAGATMSEQEKSSLVWGAKLMTLECGMRFLTDYLEGDHYFRISR
                     PAQNLDRARTQFTLVKGMEAAFADMMRIIQKY"
     gene            complement(1259..>1844)
                     /locus_tag="PLT73_08520"
     CDS             complement(1259..>1844)
                     /locus_tag="PLT73_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20453.1"
                     /translation="FAVMNADDYYGPEAFQVMYDYLSTHQDREVYDYCMVSYLLKNTV
                     SENGSVARGICVANPDGTLQSVTERTRIETHDEKICYSEDGGASWTEIGGNTPVSMNL
                     WGFTKSFLQEAENRFADWLTENLPKNPLKCEYFLPLVVSELIDEKKATVTILHSADKW
                     YGVTYREDKPTVVNAIKNKIEAGIYPMNLWEEKQ"
BASE COUNT          441 a          468 c          418 g          517 t
ORIGIN      
        1 tttctcttat ccttttaatc attttcgccg gaatcatctt tttctgttta cagtaagctt
       61 ttcacacatt tctcctttaa gatagcataa ggccgggatc gaactagcga tcccggcctt
      121 atgcattatt tcggagctgt gatttccgaa ggagaatagc atcaatattt ctgaataata
      181 cgcatcatat cagcgaaagc agcctccatc cctttaacaa gggtgaactg cgtgcgtgct
      241 cgatcaaggt tctgtgcagg gcgactaata cggaaatagt ggtctccctc aagataatcg
      301 gtaaggaatc gcattccaca ttccagcgtc atcagctttg cgccccagac cagactgctc
      361 ttttcctgct cggacatagt tgctccagcg gtcgagagga agccttttgc aaacacctca
      421 aacaagtgca ggtcaaagtg gaccttgttc tgatcgggtt catcctcggc gcagtcgttt
      481 gcgccaaaac ggatggagtc gccaaagtca tatgcagcca aaccgggcat tacggtgtcc
      541 agatcaataa cgcaaatacc ctttttactc gttttgtcga tgaggacatt gttgagtttg
      601 gtatcattgt gagtaacgcg aagcggaatc tcatttttgc tcagcatatc gaccagaaca
      661 tggcagtcag cctcacgaga gcgaacaaat gcgatttcat ctgcaacatc tttcgcacgc
      721 cccattgcat cctctgcgac agctttctca aaattctggt agcgcgtagg ggtatcatgg
      781 aatttgacaa tggtttcatg cagcgtagca gcaggaaatg cggccagctg attctggaac
      841 gtaccgaaag tttccgctac agtgtaaaaa tcattctcgt tttcgacttt ttgcagacag
      901 acagtatcct cgacaaagct gtaagctcgc catgtgccgc catcgctgtc agtatagaaa
      961 ggttttctat ctttggtcgg aataacgttc agcgtctcac gctccgggtc gccgccattc
     1021 tccagaatga ccttgcgcag gaagctggta acgttgcaga cgttttccat caattctttc
     1081 gggttcttga atgtgtcagt attgatgcgc tgcataatgt agcgcttgca tttgtcgctt
     1141 gcccaaacga caaatgtatc attgatatgg ccttcgccat agcgagaaat ttcacccgtt
     1201 gtacggtcga aggcaaatgc atcagctgct tctttcagga tgctttctgt gatagcgttc
     1261 actgtttttc ctcccataga ttcatcggat agatcccggc ttcaatttta ttcttgatgg
     1321 cattgacgac cgtgggtttg tcctcacgat aggtcacgcc ataccacttg tccgcactgt
     1381 gaagaatggt gacggtcgct tttttctcgt cgatcagctc actgacgaca agcggcagaa
     1441 aatactcgca tttcaacgga tttttcggga gattctcggt cagccaatcc gcaaagcgat
     1501 tctcggcttc ttgcaaaaag ctcttggtaa aaccccagag gttcatgcta acaggcgtgt
     1561 ttccgccgat ttccgtccaa gatgcaccgc catcctcgga atagcagatc ttctcatcgt
     1621 gcgtttcaat acgggtgcgt tcggtaacgc tttgaagtgt gccgtccgga tttgcaacgc
     1681 agatgccacg tgcgacactg ccattttccg ataccgtatt tttcagcaga tagctcacca
     1741 tgcagtagtc ataaacttcc ctatcctgat gggtggacag gtagtcgtac atcacctgaa
     1801 acgcttccgg cccataatag tcgtcggcat tcatgaccgc aaac
//
LOCUS       DAOMUJ010000385         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17831_length_1842_cov_2.653050, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000385 DAOMUJ010000000
VERSION     DAOMUJ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17831_length_1842_cov_2.653050"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLT73_08525"
     CDS             <1..700
                     /locus_tag="PLT73_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HRM20454.1"
                     /translation="RGWGQQFWGFTTEETAEAVTVTFTLDSTPETVGYPGRLQAKISY
                     TLDAEGCLTITMTGACSEETLFNPTSHIYFNLSGEAKRPITEHILQLACEEVLELDTD
                     KLPTGKKRAVDGTAFDFREATVIGDAIQKKPEGFDDVFLMHSDEQKQLVLSDAGSGRE
                     MALSSNRSSIVLFSTTDMNEPYLVNGRPMQSHLGLAIEAQEMPDAIHHPGWDNIVLAP
                     NTLATRVQNYTFKW"
     gene            737..1753
                     /locus_tag="PLT73_08530"
     CDS             737..1753
                     /locus_tag="PLT73_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20455.1"
                     /translation="MTVTIKDVAREAGVATSTVSRTLKDSPLISENTKVKVRQAMQKL
                     GYTPNFAAQNLANKSTQTIGVILPVGTMGNPAQNPLFLEMIQEIGVVCNEQKYMISLA
                     TGKTMVELKDSVRLMHQRKLVDGFILLYSEAKDPIRKFLHDEKIPYALLGKPDVYENE
                     TIYIDNDNRLSGKSAADCLIQHGHQRIGYVGLTPSQVVDKERYKGYSDALRDANLTVH
                     PELYFETPTDFMAFLEFLEETQPTGLVVGDDRLALRVLQYLQLDKYKVPDDISIVSFN
                     NSVFATLSHPFLTTIDIHVQELARQAAALLIRQLNEPSALLPKMIVPHEVIERETVMR
                     VRGG"
BASE COUNT          478 a          502 c          491 g          371 t
ORIGIN      
        1 ccgcggttgg ggccaacagt tctggggatt tacgaccgaa gaaacggcag aagcagtgac
       61 cgtcacgttc acattggact cgacaccgga aacggtcggc tatcccggcc ggctgcaggc
      121 gaaaatcagc tatacgctgg atgcggaagg ctgcttgaca atcacaatga caggcgcttg
      181 ctctgaagag acgctgttca atccgacgag ccacatctat ttcaatttaa gcggcgaggc
      241 caagcgtccg attactgagc acattttgca gctggcctgc gaagaagtgc tggaattaga
      301 tacagataag ctgccgaccg gaaaaaaacg cgccgtcgac gggacggctt tcgatttccg
      361 cgaagcgact gttatcggcg acgccattca aaaaaagccg gaaggattcg acgacgtgtt
      421 cctgatgcac tcggacgaac aaaagcagtt ggtcctcagc gatgcgggca gtggccggga
      481 aatggcgctg tccagcaacc gcagcagcat cgtgttgttt tcgacgaccg acatgaatga
      541 gccatacctt gtgaacggac gcccgatgca aagccacctc ggcttggcga tcgaagcgca
      601 ggagatgccg gacgccatcc atcatccggg ctgggacaat atcgttctgg cgccaaatac
      661 tttagcgaca agggttcaaa attacacttt taaatggtaa tctagagata caaaaatgaa
      721 cctgaggaaa atgcctatga ccgttacaat aaaagatgtc gccagagaag caggcgtcgc
      781 tacttcaacc gtctcacgca cgttgaagga cagtccgctg atcagcgaaa acacaaaagt
      841 gaaagtgcgc caagcgatgc agaaactggg ctacacgccc aacttcgccg cccagaacct
      901 ggccaataag tccacgcaga caatcggtgt gattctgccg gtgggaacga tggggaatcc
      961 tgcgcagaat ccgctcttcc tggagatgat ccaggaaatc ggcgtcgtct gcaacgaaca
     1021 gaaatacatg atttccctgg cgaccggtaa gacgatggtg gaactgaagg acagcgtacg
     1081 cttgatgcat caacgcaaac tggtcgatgg cttcatcctg ttgtattcgg aggcgaaaga
     1141 tccgatccgc aagttcctgc atgatgagaa gattccatac gcactgctgg ggaaaccgga
     1201 tgtctacgag aacgaaacaa tctatatcga caacgacaac cgcctgtccg gcaaatcggc
     1261 ggccgactgc ctgatccagc atgggcacca gcggattggc tatgtcggtc tgacgccttc
     1321 ccaagtcgtc gacaaggaac gctataaagg ctattccgat gccttgcgcg acgccaatct
     1381 gaccgtccat ccggaactct atttcgaaac gccgactgat ttcatggctt tcctggagtt
     1441 cctcgaagag acccaaccga ccggactcgt cgtcggcgat gaccgcctag ctttgcgtgt
     1501 cctgcagtat ctgcagctgg acaagtacaa agttccggac gacatctcca tcgtcagctt
     1561 caacaattcc gtctttgcga ccctgtccca cccgttcctg acgaccatcg acatccacgt
     1621 ccaggaactg gcccgacaag ccgccgccct cctgatccgc caactcaacg aaccgagcgc
     1681 cttgctgccg aagatgatcg tgccgcatga ggtgatcgaa cgggagacgg ttatgcgggt
     1741 gcggggggga taaactcaat aagactgcca caaaagggct tttatccaat gcgataaagg
     1801 tccttttttc gtgcaggttt taagccgatt tgcattattt gt
//
LOCUS       DAOMUJ010000386         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17832_length_1842_cov_2.187465, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000386 DAOMUJ010000000
VERSION     DAOMUJ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17832_length_1842_cov_2.187465"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..256)
                     /locus_tag="PLT73_08535"
     CDS             complement(53..256)
                     /locus_tag="PLT73_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HRM20456.1"
                     /translation="MSADLQPYFPILIPLVVIQIILLITALLHLNKHPHVKIGNKNLW
                     IFVIIFLNIIGPVLYFLIGRGDE"
     gene            522..1442
                     /locus_tag="PLT73_08540"
     CDS             522..1442
                     /locus_tag="PLT73_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM20457.1"
                     /translation="MNPLFKLMLKLMSSPKINMQEDYARVRKVQKILAPKPKKGYIIM
                     DHKIYSEDRSHDIPVRVFYPKEHLYKEPLLFFHGGGWVIGDIETYTNACINMADLTGR
                     TVYSVDYRLAPEHPYPAGLYDCYRVAEVLLKHLEMSGLSDEKDLILIGDSAGGNLAAA
                     VSLLLRDRGQATPIKQILLYPVTYWDHSEASPFESIRTNGQDYGLTAKKMEEYMALYQ
                     PDPELRKNEYVAPLMAENLSNQPETLVITAEFDPLRDEGEAYAEALQKAGNKVQVHRV
                     NGIVHGFITYPKTAESVEEAYEVINAFLNA"
     gene            1491..>1842
                     /locus_tag="PLT73_08545"
     CDS             1491..>1842
                     /locus_tag="PLT73_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HRM20458.1"
                     /translation="MNRKKWVRLDNASNIFLAAMTNSDTKVFRMSAELTDAVDPLLLQ
                     RALDEVYENYVLYHSVLRRGFFWYYLEESDLKPKVTADTLPPCAQIYHFDRKELLFRV
                     FYNQNRVHLELFHAL"
BASE COUNT          510 a          397 c          460 g          475 t
ORIGIN      
        1 acgctttggc tcccgaaggc tttccccaat ccggcaatcg tgaggatgtc catcactcat
       61 cgccccttcc aatcaaaaaa tacaggaccg gaccgatgat gttcaggaaa atgatgacga
      121 agatccaaag atttttattg cctattttta catggggatg cttgttcagg tggagcaggg
      181 ccgtgatcaa caggatgatc tggatcacga ctaaagggat caggataggg aagtatggct
      241 gcaaatcagc tgacataaaa actcgccttc tttctttttt ttcgcttagt tttatatctc
      301 caggttaggt ttacgattgg gttcaacaaa accactcccg tgatcgttcg ttatataaca
      361 agtataacaa tttaggcgga atttgcaagc cgaaagagtg gcatatccat cacacctgta
      421 gccttcacga agggtccaag tgtgatggga ctagctcatc ctgtgctata ataaagcaaa
      481 gataccagaa cagccaacgc tgtcagagga aggaaaattg aatgaatcca ttgtttaaat
      541 tgatgctcaa gttgatgtcg tcaccaaaaa ttaacatgca ggaagattat gcccgcgtcc
      601 gcaaagtgca gaagattttg gctcccaaac caaaaaaggg ttatatcatt atggatcaca
      661 aaatctattc ggaggaccgt tcccatgata ttcccgtgcg tgtcttctat ccgaaagagc
      721 atctgtataa agaacccttg ctctttttcc atggcggtgg gtgggtcatc ggtgacatcg
      781 aaacctatac gaatgcctgc atcaatatgg cggatctgac cggtagaacg gtctactctg
      841 tcgattatcg tttggcgccg gagcatccgt atccggcagg cctctatgat tgttaccggg
      901 tagcggaagt attgctgaag catctggaaa tgagcggctt atcggatgaa aaagatttga
      961 ttctgatcgg cgattcagcc ggtgggaatt tggccgcagc cgtgtctttg ctgctccgtg
     1021 atagaggtca agcgacgcca atcaaacaga ttttgttgta tccggtcacc tattgggatc
     1081 attcggaagc ttcccctttc gaatcgatcc gtacgaatgg acaggattac ggactgactg
     1141 ccaaaaaaat ggaagaatat atggcgctat accagcctga tcctgaactt cgtaaaaatg
     1201 agtatgtcgc acctttaatg gctgaaaatt tgtcgaatca gccggaaacg ttggtgatca
     1261 cagccgaatt cgatccgttg cgggatgagg gcgaagccta cgcggaagcg ttgcagaaag
     1321 ccggcaacaa ggtgcaggtt catcgcgtga acggtatcgt ccatggcttc atcacctatc
     1381 cgaagacggc cgaatcggtt gaagaagcct acgaagtcat caatgcgttt cttaatgcat
     1441 aagccaagaa gctcgttcta tagaaaagaa ggaagaaagg aggggcagca gtgaaccgaa
     1501 aaaagtgggt acgtttggat aatgcctcca acatattttt agcggccatg acgaacagtg
     1561 atacgaaggt cttccgcatg agtgcggaat tgaccgatgc ggtggatccg ttgttgctgc
     1621 agcgggcact tgatgaagtc tatgagaact atgtgctgta ccacagtgta ctgcggcgtg
     1681 gatttttctg gtattatctg gaagaaagcg atctgaagcc aaaagtgacg gccgacacgt
     1741 tacctccttg cgcacaaatt tatcattttg atcggaagga actgttattt cgagtcttct
     1801 acaaccaaaa tcgggttcat ttggaattgt tccatgcctt gt
//
LOCUS       DAOMUJ010000387         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_17965_length_1832_cov_1.935847, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000387 DAOMUJ010000000
VERSION     DAOMUJ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17965_length_1832_cov_1.935847"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLT73_08550"
     CDS             <1..266
                     /locus_tag="PLT73_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM20459.1"
                     /translation="NKVSVVFSLDNQAGTLFKLLRHFARYHINLIKIESRPVEGEKWE
                     YLLYLDFEGNISDENVHEALRFIHRSSVYFRLLGCYIAATENK"
     gene            388..912
                     /locus_tag="PLT73_08555"
     CDS             388..912
                     /locus_tag="PLT73_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HRM20460.1"
                     /translation="MKAAERRNEIIKLLEASDQPVNATQLAERLSVSRQVIVGDIALI
                     RAKGIEILATPRGYLSASAFKGEGSLFTGKIACQHSQSDTVTELNIIVDNGGEVVDVQ
                     VEHPVYGTLTGELHIRSRHDVIDFMKKIASNEAAMLSSLTGGVHLHTLACNDEETFLR
                     IKMELQEAGILYSG"
     gene            1008..1565
                     /locus_tag="PLT73_08560"
     CDS             1008..1565
                     /locus_tag="PLT73_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20461.1"
                     /translation="MNTRTQTKHITIAALLIAIGILIPMISPLKIILEPASFTLASHV
                     ATMIAMFLSPGIAVAVAFGTAVGFMLGGFPLVVVLRAATHMIFSYVGAKYIQKHPEMA
                     HDIKKSLLFSFVLGVLHAGCEVIVVSLFYFAGNLTEAYYDKGLISSVMLLVGVGTVIH
                     SMVDYWLAQVVWKTVGKKALPVRSV"
BASE COUNT          495 a          415 c          456 g          466 t
ORIGIN      
        1 gcaataaagt cagtgtagta ttttccttgg acaatcaggc cggcacgctt ttcaagctgc
       61 tgcgtcattt cgcgaggtat catatcaatc tgatcaaaat cgaatcccgc ccggtcgaag
      121 gggaaaagtg ggaatacctg ctgtacctgg attttgaagg caacatctcc gatgaaaacg
      181 tccacgaagc cctgcgcttc atccatcgaa gcagcgtcta tttccgttta ttgggctgct
      241 acatcgccgc aacggaaaat aaataagaaa agccagcaat acctgccttg tgcggtgcgt
      301 tgctggcttt ttgtttttcg aatcttgcag tccagatcaa aaatgggtac tattaagaag
      361 agaaagcgtt agggaagggt gagtttattg aaagcagcag agagacggaa tgaaatcatc
      421 aaattattgg aagccagcga tcaaccggtg aacgccaccc aactggctga aagattatcg
      481 gtcagccgtc aggtcatcgt gggggatatc gcactgatcc gtgccaaagg gattgaaatc
      541 ctggcaacac cgcgaggcta tctatccgcg agtgccttta aaggtgaagg cagcctgttc
      601 acggggaaaa ttgcctgtca gcattcgcaa tccgatacag tcactgaact gaacattatc
      661 gtagacaacg gtggcgaggt cgttgacgtt caagtcgagc atcctgtcta tggcacattg
      721 acaggagaac tgcatattcg atcgcgccat gatgtcatcg attttatgaa aaaaattgcc
      781 tcaaacgaag cggcgatgct gtcttcgctg acaggcggcg tgcatctgca tacacttgct
      841 tgtaacgatg aagaaacctt cctgcgcatc aaaatggaac tgcaggaagc gggaatcctg
      901 tattccgggt aagctacctg agaataagct tgacaactcc tgaaaagatt atataatata
      961 ttacaactgt caagacagtt gtaaacatct ttggagggat tggccgaatg aatacacgta
     1021 cacaaacgaa acacattacg atagctgctc tactgattgc gatagggatc ctgataccga
     1081 tgatttcccc tttgaaaatt attctggaac cggcatcctt caccttggct agccatgtgg
     1141 caacgatgat tgctatgttc ctttcgccag gcattgcggt tgcggttgcc ttcggaacag
     1201 ctgtcggctt catgttagga ggctttccgt tggtcgtagt tctgcgtgcg gcaacgcata
     1261 tgatcttttc ttatgtcggc gcaaaatata tccaaaagca tccggaaatg gctcatgaca
     1321 tcaaaaaatc attgctgttc agcttcgttc ttggtgtatt gcatgctggc tgtgaggtca
     1381 ttgtcgtaag cctgttctac tttgcaggaa accttacgga agcgtactat gataaaggtc
     1441 tgatttcttc cgtcatgctg ttggtaggcg taggaaccgt catccacagc atggtcgatt
     1501 actggttggc ccaagtcgtc tggaaaacag tcgggaaaaa agccctgcct gtacgttcgg
     1561 tgtagatcgg aaaatgaatg aaataaaaag gacgcatccg gcttcaagta ccggatgcgt
     1621 ccttttatta ttggggtgtc tgaagagtac aacagctgcc gccctggctg tcgggcattc
     1681 cgagcgtgat tcatcgtgaa cagtcgagaa agcagccatc tgtctactgt tccgtgtcca
     1741 cagccagcca gataaccgac agccaatcat tcagccagta agaaaagctg aacgggttcg
     1801 ttcagccctg aaagaaattt aggaaatctg tc
//
LOCUS       DAOMUJ010000388         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18031_length_1826_cov_1.764540, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000388 DAOMUJ010000000
VERSION     DAOMUJ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18031_length_1826_cov_1.764540"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLT73_08565"
     CDS             complement(<1..1052)
                     /locus_tag="PLT73_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20462.1"
                     /translation="MNNQQGATRIQGIIVPLLSVILGLVIGAIVMWSFGYDAIAGYSA
                     MLKGAFGSPFYIGELLREATPLILVALGFAVANTAGFFNIGVAGQTLFGWLASVSVAQ
                     ILPDLPKMILLPLCILAGVAAGAIWAGIAGVLRAYFNTSEVIVTIMLNHTALYINNHI
                     VRNVLTDSGDSTARITENASLRVPFLSELTRNSTLHAGIFIALIMIAVVWVLMKKTTY
                     GFEFRSVGLNPDAADYAGMNAKKNIILAMLISGGLAGLGGAMEGLGNFQNMFVQGAMP
                     AVGFDGMAVALLGIGSPIGILFAALLFSTLKIGGTSMPLMSGVPVEIVDIVIASIIFF
                     VGASYIIRLLVNKLPKV"
     gene            complement(1045..>1826)
                     /locus_tag="PLT73_08570"
     CDS             complement(1045..>1826)
                     /locus_tag="PLT73_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20463.1"
                     /translation="TVKESRGLEAVRNLNLTVRAGEIVGIAGIDGKGQSELIQAITGL
                     RKAESGTVKLNGEPITNLAPRKITEAGLGHIPEDRQKFGLILPMRLDENIALQTYYQE
                     PYSKNSFLNDKAIENHAIELIKEFDVRTQSEKSTASSLSGGNQQKAIIAREVDRNPSL
                     LIAAQPTRGLDVGAIEFIHKRLIEQRDKDKAILLMSFELDEILNLSDIIAVMFEGEIV
                     AYVKPEETSEQELGLLMAGSSLEKAREDLEKNKDEEGSLHE"
BASE COUNT          463 a          478 c          401 g          484 t
ORIGIN      
        1 actttgggca gtttgttgac caatagtcgg atgatatagc tcgcgccgac gaagaagatg
       61 atcgatgcta tcacaatatc gacaatctct accggcactc cggacattag cggcatgctg
      121 gttccgccaa ttttcaaggt actgaacaga agtgcagcaa aaaggattcc aatcgggcta
      181 ccgattccca ataaggctac tgccatgcca tcgaatccga cggccggcat tgctccttga
      241 acaaacatgt tctggaaatt tcccaaccct tccattgctc ctcctaaacc ggccaatccg
      301 ccgctgatta gcatggccaa aatgatgttc tttttggcat tcatccctgc gtaatcagcc
      361 gcatcaggat tcaatccgac tgaacggaat tcaaaaccat atgttgtttt cttcatcaac
      421 acccatacca ccgcaatcat gatgagagca atgaagatac cggcatgcag tgtcgaattg
      481 cgggtcagtt ccgacaagaa cggtactctc agactggcat tttctgtgat tctggctgta
      541 gagtcaccgg aatccgtcaa aacattcctc acgatgtggt tattgatgta taaagccgta
      601 tgattgagca tgatggtgac tattacttca ctagtgttga aataggcacg taagactccg
      661 gcaatgcccg cccatatagc tccagctgca acgccagcta aaatacacaa cggcaacaag
      721 atcattttag gcaaatcagg caatatttgg gcgacgctga cggatgccaa ccagccaaac
      781 aaagtttgac ctgccacacc gatgttgaag aacccggctg tgttggcaac agcaaaacct
      841 aatgcgacca gaatcaaagg agtagcttca cgcagtaatt ctcctatgta aaacggcgat
      901 ccgaatgcac ccttcagcat ggctgaatag cctgcgatgg catcgtaacc gaaagaccac
      961 atgacgattg ccccgattac cagacctaat atgactgaaa gtaaaggtac gataatccct
     1021 tggatgcgag tggctccttg ttgattattc atgaagactt ccctcctcgt ccttgttttt
     1081 ttctaaatct tcccgtgctt tttccaaaga agagccggcc atcaatagcc cgagttcttg
     1141 ttcggaagtt tcctccggct tgacgtaggc cacaatttcc ccttcaaaca tcacggcgat
     1201 gatatcggag agattcaaga tttcatccaa ttcgaagctc atcagtagga ttgccttatc
     1261 tttatcccgc tgctcgatca aacgtttatg gataaattcg attgcgccca catccaatcc
     1321 ccgggtcggt tgggctgcga tcagtaacga aggattccga tcgacttccc tggcgatgat
     1381 cgccttttgt tggttcccgc cggataatga gcttgccgta gatttttcac tttgggtccg
     1441 cacatcaaac tctttgatca gttcaatggc atggttttcg attgctttat cgttgagaaa
     1501 gctgtttttg ctgtaaggct cctgataata agtctgcaag gcgatgttct catccagacg
     1561 catcgggaga atcaggccga acttttgtcg gtcctcagga atatgcccca gcccggcctc
     1621 ggtgatttta cgtggagcca agttcgtgat aggttcgcca ttcagcttga cggttccact
     1681 ctcagctttt ctaaggcctg tgatggcttg gatcaattcg ctctgaccct tcccatcgat
     1741 tccggcgatt ccaacgattt caccggcccg tactgtaagg ttcaaattgc gtactgcttc
     1801 cagaccacgg ctctctttaa ccgtca
//
LOCUS       DAOMUJ010000389         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18042_length_1825_cov_1.745198, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000389 DAOMUJ010000000
VERSION     DAOMUJ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18042_length_1825_cov_1.745198"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..689
                     /locus_tag="PLT73_08575"
     CDS             171..689
                     /locus_tag="PLT73_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20464.1"
                     /translation="MFQKSQKVNIDDTRFIFQTNFSGDPARDRFGSDKRRVNIVIPTV
                     EQAMDMKAMGINVKETHPNPNYTYDEPFVPTYYVPVTVNVDSKWPPHVYWIPLQGKRL
                     LCTPETIGQLDFIRVKNVCCQANLVEKRNAPGEYTLYADVMYVEQAPDNDPYADRYAQ
                     MAEPNDPNNVPF"
     gene            700..1083
                     /locus_tag="PLT73_08580"
     CDS             700..1083
                     /locus_tag="PLT73_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20465.1"
                     /translation="MKKLFISCPMRGRTENAIKASMEQMHRIAEAVFGEELEVIPTYF
                     EGDPPENSNQALWYLGESIKKMSEANYFIGIYDEDKSYRGCIIENRTAKSYGIPSYIV
                     NISFIAPDVIEQKSIDKRVANLEIY"
     gene            1208..1582
                     /locus_tag="PLT73_08585"
     CDS             1208..1582
                     /locus_tag="PLT73_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3846 domain-containing protein"
                     /protein_id="HRM20466.1"
                     /translation="MKVLRIQPKKYPEVIEIDGSLKSLQKEVAGPIQAVYPWDDPVAL
                     ICNEEGKLIGVSFNRTLMDADGTPTDVIAGTFLIVGLTEDDFGDLSQEYIDKYEKLFH
                     QPEKFWHEIDERGRTRLKNRLL"
     gene            1653..1731
                     /locus_tag="PLT73_08590"
     tRNA            1653..1731
                     /locus_tag="PLT73_08590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Ile,seq:cat)
BASE COUNT          504 a          429 c          473 g          419 t
ORIGIN      
        1 tttatggact gctatgaaga aggctgcatg gcatatcggg ctgaagcaaa agattccgac
       61 ggaggagtcc gagtaaaggc tggctgccgg ttgatcgacg aatacgttga gcactctacg
      121 ccgttcaata actacaacac attataaata aggtaaggag attgatattt atgttccaga
      181 agagtcagaa agtaaacatc gatgataccc gtttcatctt ccagaccaat ttttccggtg
      241 atcccgcccg tgaccgtttc ggctcggaca agcgccgcgt caacatcgtg atccctacgg
      301 ttgagcaggc catggatatg aaggcgatgg gcatcaacgt taaggagacc catccgaacc
      361 ccaactacac ttatgacgaa ccgtttgtgc cgacctacta tgtgccggtc acggtcaatg
      421 ttgattccaa gtggccgccg catgtctact ggatccccct gcagggcaag cgccttctgt
      481 gcactccgga gacaatcggt cagctcgact ttatccgtgt caagaacgtc tgctgccagg
      541 caaatcttgt cgaaaagcgt aatgctcccg gtgagtatac tctgtatgct gatgtgatgt
      601 acgtggagca ggccccggac aacgacccgt atgcagaccg ttacgcacag atggccgaac
      661 ccaacgaccc gaacaatgta ccgttctaag gaggagcaca tgaagaaact gtttattagc
      721 tgtccgatgc gtggtcgcac ggaaaacgcc atcaaagcat ccatggagca gatgcatcgg
      781 attgcagagg ccgtctttgg cgaagaactg gaggtcattc cgacttattt tgagggcgat
      841 cctcctgaga atagcaacca ggctctctgg tatctcggtg agagcatcaa aaagatgtcg
      901 gaagcaaact actttatcgg catctacgac gaggacaaga gctatcgcgg ctgcatcatc
      961 gaaaaccgca cggcgaaaag ctacggcatt ccgtcctaca tcgtgaacat cagcttcatt
     1021 gcaccggatg tcatcgaaca gaagagcatt gataaacgtg tagcaaacct cgaaatttat
     1081 taaatgatat ttctgagtgc cgaggttagt cctcggttga atgtccagcc agtgagtgcc
     1141 cacgtcgcaa atggcgttct cagaggaaac agctcgattg atattttgat tttgggaggt
     1201 tgaacgtatg aaagtcttga gaatccagcc caagaagtat cctgaagtta ttgaaatcga
     1261 cggctcgctc aaatctcttc agaaagaagt ggccggcccg attcaggcgg tctacccgtg
     1321 ggacgatccg gttgcactta tctgcaatga ggaaggaaaa ctgattggtg ttagctttaa
     1381 ccggactctc atggatgcag atggtacacc tactgatgtg attgccggta cattccttat
     1441 tgtagggttg acggaagatg acttcggcga cctttcgcag gagtacatcg acaaatatga
     1501 gaagctgttc caccaaccgg agaaattctg gcatgagatc gacgaacggg gaagaactcg
     1561 tctgaaaaat cgattgctgt gatccggagg agtaagacac tctaaaatca aacaacctgg
     1621 agccgtggag aaatctgcgg ctcttttatt ttgggtcagt agcttagcca ggttcaaagc
     1681 cggcagctca taactgcttg atcgcgggtt caaatcctac ctgacccacc agaggtgcaa
     1741 gccttatatt tgaataaaca aaggagagaa cagcatgagc gcaagaaact atgccccgga
     1801 aattgtgaaa tggatggtcg gggaa
//
LOCUS       DAOMUJ010000390         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18099_length_1821_cov_2.127407, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000390 DAOMUJ010000000
VERSION     DAOMUJ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18099_length_1821_cov_2.127407"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLT73_08595"
     CDS             <1..181
                     /locus_tag="PLT73_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20467.1"
                     /translation="HSSGCVILFIKFFRQEGFISFFNPLLHKLDRELETFGLEFFFTT
                     ETNWLNKIRFAVDDS"
     gene            complement(209..>1821)
                     /locus_tag="PLT73_08600"
     CDS             complement(209..>1821)
                     /locus_tag="PLT73_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRM20468.1"
                     /translation="ATVFYGNGIVLPTNQLTLSEIRSLDQSLKGGHVCMGQVDDIVAC
                     ITTLNVDLEAFYTHLFGSEHYWRKAKEHKGEAIIKDIIEYKNHYIPDDVIKDYGPKIG
                     LRIDSYRIIFSIDNPNAKEMMRAMCNYYDTSTKAFVFKRPIDRDELYDLDVMSEIQSN
                     ITVFLTEARHYCSENEIHVDTVFRYKTSNRQSVRYVFNNVTQYVAQRDNMSFNPDTGN
                     LGIDFNWRSDNIWAILKELEKSVPFISARTYGAEHKFKCNVSTSMVGFSEVENRLNDR
                     FENVAIENDANSHCIRISLPCPSVDYYERLHDSLQRELSFLRGSCNVLLDQRVSGKVK
                     VLIHRDEKSRIEDIEDNLKDMRKADFGFEVGEKMVPFGNLLSVKYPKLLFDIDVEPGK
                     EQAVIKYLNQKSIQFVVPILTGDIEKISRLKNTFTMATSGNNLLNDNLQNFIFDSALA
                     TPTPDLQYILRRDGGPYQELCRHLINSRINESQKDAILKCIFAKDLAVIQGPPGSGKS
                     TAIAELIWQLIRNGLKQGNKCERILLTS"
BASE COUNT          494 a          406 c          383 g          538 t
ORIGIN      
        1 acacagttct ggttgcgtca tcctcttcat caagttcttc cggcaggagg gcttcatctc
       61 cttcttcaac ccacttcttc ataagctcga tcgagaactg gagaccttcg gactcgagtt
      121 tttcttcacc accgaaacga attggcttaa caagattcgt tttgctgttg atgattcttg
      181 aaatagcatt gtcaacagca aggttcgttt atgaagtaag aagaatcctt tcgcatttgt
      241 taccttgttt cagtccattc ctgatgagct gccagatgag ttcagcaatt gcagttgact
      301 taccagaacc cggaggccct tgtatgacag cgagatcttt tgcgaagata catttaagaa
      361 ttgcatcctt ctgtgactca ttaatccgcg agttaatcaa atgtctacag agttcttgat
      421 atgggccgcc atctctccgt aatatgtatt gtaaatcggg agtcggtgtg gcgagcgcag
      481 aatcaaatat gaagttctga aggttgtcgt taagcagatt gttgccactt gtggccatcg
      541 taaacgtatt cttcaacctg gaaatctttt cgatatcccc ggtaagaatg ggaacaacaa
      601 actgaatgct cttttgattg agatacttga tgacagcttg ctcctttccg ggctcaacgt
      661 ctatgtcgaa taacagcttt ggatacttaa ccgacagcag attgccgaaa gggaccatct
      721 tttcgcctac ttcaaagccg aaatctgcct tgcgcatatc cttgaggtta tcctctatgt
      781 cttcaatacg agatttttca tcgcggtgaa ttaggacttt gactttcccg gaaacgcgct
      841 ggtcaagaag tacattgcaa gatcctctta ggaaagaaag ctccctctgc agagaatcat
      901 gtaatctttc ataataatca acagaaggac aggggagact gatgcggata cagtggctgt
      961 tcgcatcatt ctctatggca acattttcaa atctgtcgtt tagccggttc tccacctcgc
     1021 tgaacccaac catcgaagtg gaaacgttgc atttgaactt atgctcagca ccgtatgtgc
     1081 gagcagaaat gaaaggaact gacttttcca gttctttaag aatagcccag atgttgtccg
     1141 accgccaatt gaaatcgatc ccaagatttc cagtgtcagg attgaaactc atattgtcgc
     1201 gctgcgccac atattgagtc acattattga aaacataacg gacggattga cggttggatg
     1261 tcttatagcg gaaaaccgta tcaacatgaa tctcattttc cgagcaatag tggcgggctt
     1321 cagttaagaa aacagtgata ttactttgga tctcggacat cacgtcaagg tcgtataatt
     1381 catcccgatc aatgggacgc ttaaagacaa atgcctttgt ggatgtgtca taataattac
     1441 acatggccct catcatctcc ttagcgttcg gattgtcgat actaaaaata atccgataac
     1501 tatcaatcct taagccgatt ttgggaccat agtctttaat aacatcatcg ggaatataat
     1561 gattcttata ctcgatgatg tcttttatga tggcttctcc tttgtgctct tttgcctttc
     1621 tccaataatg ctcggaacca aaaaggtgag tatagaatgc ctctaggtca acattaagcg
     1681 ttgtgatgca ggcaacaata tcatcaactt gccccataca tacatgccct ccctttaaag
     1741 attggtcgag gctgcggatt tctgagagag tgagctgatt agtcgggagt actatcccgt
     1801 ttccgtagaa aacggtcgca c
//
LOCUS       DAOMUJ010000391         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18105_length_1821_cov_1.463760, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000391 DAOMUJ010000000
VERSION     DAOMUJ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18105_length_1821_cov_1.463760"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLT73_08605"
     CDS             <1..358
                     /locus_tag="PLT73_08605"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRM20469.1"
                     /translation="VGPEARGFMVGCPVAVELGCGFAMARKKGKLPRETVEVSYGLEY
                     GKATLQLHQDAILPGDKVLVCDDLLATGGTTAATIELVEKLGGEVVGLAFLIELLDLK
                     GRDLIQGYDIFTLMEY"
     gene            complement(440..1063)
                     /gene="lexA"
                     /locus_tag="PLT73_08610"
     CDS             complement(440..1063)
                     /gene="lexA"
                     /locus_tag="PLT73_08610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRM20470.1"
                     /translation="MVKNKDSRQIEVLQYIYEEVQEKGYPPTVREIGNAVKLSSTSTV
                     HGHLSRLEKNGFIQRDPTKPRAIELTQAGLDKLGILSDKMPLLGTVTAGAPILAVEEA
                     TDYFPVPPDLQSNAGSLFMLKIRGESMIDAGIFDGDSVIVRKQNTAENGDIVIAMTDD
                     DEATCKRFYKENNYYRLQPENSSMDPIILDSVLILGKVVGLYRNHII"
     gene            1308..1538
                     /locus_tag="PLT73_08615"
     CDS             1308..1538
                     /locus_tag="PLT73_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HRM20471.1"
                     /translation="MSTTAWVMMVILGIVIGAVGGFFLARRYMVKYFEENPPINEEML
                     RSMMMQMGQKPSERKVKQIMASMKAQSTKKKK"
     gene            1625..>1821
                     /locus_tag="PLT73_08620"
     CDS             1625..>1821
                     /locus_tag="PLT73_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HRM20472.1"
                     /translation="MSIFKKLSWFFRQEWKAYFVGVFFLIIVAILQVVSPRIVGIIID
                     EIALGTLTMASLWKWTAIILIA"
BASE COUNT          504 a          352 c          433 g          532 t
ORIGIN      
        1 agtcggacct gaagcgcgtg gcttcatggt cggttgccct gtggctgtgg aattaggctg
       61 cggattcgcg atggcccgca aaaaaggcaa attgccccgc gaaacagtag aagtatccta
      121 cgggctggaa tacgggaaag cgacattgca actgcatcaa gacgccatct tgcctggcga
      181 taaagtgctg gtctgtgatg atttgttggc tactggcggc actacagccg cgactataga
      241 attggttgaa aaattaggcg gagaagttgt gggcttggcg ttcttgatcg agttgctgga
      301 tttaaagggc agagatctga tccaagggta tgacatcttc acgctgatgg aatactaaga
      361 gaagcaatcc taaataagca tagacacgca aaaaggctgc cggttggcag cctttttgcg
      421 tcagttggag gactgttctt taaatgatgt ggttccggta caggccgact actttaccca
      481 ggatgaggac agaatccagg atgatcggat ccatgctcga attttcaggt tgcagacgat
      541 agtagttatt ttctttgtag aaacgtttgc aggtcgcttc atcatcgtcc gtcatggcta
      601 tgacgatatc tccgttttca gccgtgttct gttttctgac aatgacggag tcgccatcga
      661 agatgccggc atcgatcatg ctttctccgc ggattttcaa catgaataaa gatcccgcat
      721 tggattgcag atccggggga accgggaaat agtcggttgc ttcttctacg gcaagtatgg
      781 gcgctccggc tgtgacagta cccaacaacg gcattttatc tgataggatc cccaatttgt
      841 ccagacctgc ttgggtcaat tctatcgcac ggggttttgt aggatcccgc tggataaaac
      901 catttttttc aagacgcgac agatggccat gaaccgtaga tgtagatgac agttttaccg
      961 catttccgat ttcccttacg gtcggcgggt agcccttttc ctggacttct tcataaatgt
     1021 attgaagcac ttcaatttgg cgtgaatctt tatttttaac catgagatca tcatctccgt
     1081 aatcctatat ttatatcgtt taggatacca ttaatccccc aaaaaatcaa acagatgttc
     1141 gcatgttctt tgaaaattat gtaagaatat gcctagtatt gccacaaatc cagcaaatgt
     1201 tgaagcgttt tccaaaaatc gctaaattta tccgtgattg ctatttcttt ttctcataat
     1261 ctttagtaca attaatgaat gaatgctttg gaaaggggga tgaatacgtg tcaacaacag
     1321 catgggtaat gatggttatt ttaggaatag taataggcgc ggttggcgga tttttccttg
     1381 caagacgtta tatggtaaaa tattttgaag aaaatccacc gatcaatgaa gaaatgttgc
     1441 gttctatgat gatgcagatg ggtcaaaaac catccgaaag aaaagttaaa caaattatgg
     1501 cttcgatgaa agcacaatca acaaagaaaa agaaatagac taatgaaata atatacaaaa
     1561 tgtggatctt tacggttcca catttttatt ttgtctgaat aaacgcaaga aaggggaaat
     1621 atccgtgagt attttcaaaa aattgagttg gttttttcgt caggaatgga aagcctattt
     1681 tgtgggcgtc tttttcctga tcattgtggc tatacttcag gttgtatcgc cgcgcatcgt
     1741 cggaatcatt atcgatgaaa ttgcacttgg cacgttaacg atggcttctt tgtggaagtg
     1801 gacagcgatc atattgatag c
//
LOCUS       DAOMUJ010000392         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18152_length_1817_cov_1.979001, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000392 DAOMUJ010000000
VERSION     DAOMUJ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18152_length_1817_cov_1.979001"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLT73_08625"
     CDS             complement(<1..523)
                     /locus_tag="PLT73_08625"
                     /inference="COORDINATES: protein motif:HMM:NF021197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2397 family protein"
                     /protein_id="HRM20473.1"
                     /translation="MSILADAIEYSKINEASYLSADNFQRYRTIMHFFYQQHRKMNDL
                     LYRGDVLEYMQSLDGFAGYSEKELDQDLTSLVGWENLETRQEMSEPKSIAEYKNKHFR
                     YQISETSIAIEEMLEKLSGKTGHSRGALDKNTFERFLSAIRELDQQSDEKELFNIWQD
                     TVTHFTSIKTNTSD"
     gene            744..1593
                     /locus_tag="PLT73_08630"
                     /pseudo
     CDS             744..1593
                     /locus_tag="PLT73_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344910.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
BASE COUNT          452 a          447 c          428 g          490 t
ORIGIN      
        1 agtcggatgt attggttttg atgctcgtaa aatgcgtgac ggtgtcctgc caaatattga
       61 acagttcctt ctcgtcggat tgttgatcca actcccttat ggctgacaga aacctttcaa
      121 aagtattctt atccaaggcc cctctgctgt ggccggtctt gccgctcaat ttctccagca
      181 tctcttcgat cgcgatgctg gtttcggaaa tctggtagcg gaaatgttta tttttgtatt
      241 ccgctatgct cttgggttcg ctcatttcct gacgcgtctc caggttttcc catcccacca
      301 ggctggtcag atcctgatcc aattcttttt cggaatagcc cgcaaatcca tccagactct
      361 gcatgtactc cagcacatct ccccggtaca gcaaatcgtt catcttccga tgttgctgat
      421 agaaaaagtg catgatggtg cggtagcgct ggaaattgtc tgctgagaga tagcttgctt
      481 catttatttt actgtactcg attgcgtcgg ccagaatact cactccttga tcatgaatta
      541 tatgtaaaaa gcttcccata ttaatatccg tagttcgttg ttcccacatc aaaatatagc
      601 gatacatcca ttttatcaca atagttccaa attttttcct ggagaatgaa ctagtgctat
      661 actgacctta gaaatcctga ttccacgaaa aagcagggcc ggattcggat ttcggaaatc
      721 tgaaaatcga aggagtttgt atcatgaaca aagaacaatt cgatatcatg aaaaatcgca
      781 aaggtttcat tgcggcgttg gaccaaagtg gcggcagcac gcccaaggct ctggcgttgt
      841 acggtatttc ggaagatagt ccatgagatg cgctcgcgca tcgtcacctc gccggctttc
      901 agttcggaac acatcttggg agccatcctg ttcgaacaaa cgatggaccg caaaatcgat
      961 gacctctaca cggccgacta cctatgggaa aagaaacgca tcgtaccctt cctgaaagtc
     1021 gataaagggc ttgatgcgga agctgatggc gtccagttga tgaaaccgaa tcccgatctg
     1081 aacaccctgc ttgcgcacgc aaacgagcgc cacatcacca aaatgcgttc cgtcatcaaa
     1141 tctgccaatc gtgaaggcat caaaaaagtc gtcgcccagc aattcgaagt cggcaagcag
     1201 atcattgctg cgggactgat cccgatcatc gaaccggaag tcgacatcca tagtgcagac
     1261 aaagctgaat cggaaacgat cctcaaggaa gaattggtgt tggcgctgga tgggctgctg
     1321 gcggatcagt tcgtgatgct gaagctttcg attccaacgg ttgatgattt ctacaaggaa
     1381 ctgatcgctc atccgcaagt cgtgcgggtc gtggcactat caggcggcta ttcgcgtgaa
     1441 gaagccaacg aaaaattgac ccacaatccc ggattgatcg cgagcttctc gcgggcgttg
     1501 tccgaaggtc tgagcgccgg tctgaccgag gatgaattcc gaaacacctt ggccgaatcg
     1561 atccaatcca tctacgacgc gtcgattgtt tgatgggtta tgcaaagata aatgcctttt
     1621 tgtgccttcc ggtagattga aaaactgaat aattattgct tatccgacaa cttgcgcaac
     1681 gtttgctctc cggttgtcgg attcttcgtg gttacccgac aaatcacaga gcgtttgctc
     1741 tcaggttgtc ggattcttcc gggttatccg gcaagtcaca gaatgtttgc tctccggttg
     1801 tcggattctt cgtggtt
//
LOCUS       DAOMUJ010000393         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18206_length_1814_cov_1.783968, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000393 DAOMUJ010000000
VERSION     DAOMUJ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18206_length_1814_cov_1.783968"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>390
                     /locus_tag="PLT73_08635"
     CDS             <1..>390
                     /locus_tag="PLT73_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRM20474.1"
                     /translation="IEERDHPEHRGKPIVIANHPRETGRKGVVTTANYIARKFGIHSA
                     MSAQKAFELCPEAIFISPRMNHYKMISRQIRGIFARYTDLIEPLSLDEAYLDVTQNKQ
                     NLSSATVIARRIQREVWQETGLTCSAGV"
     assembly_gap    391..400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <401..1032
                     /locus_tag="PLT73_08640"
     CDS             <401..1032
                     /locus_tag="PLT73_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRM20475.1"
                     /translation="KIASDYQKPAGLTVITPDEAARFLWELPIEKFYGVGKKTVEKMK
                     LLGIHAGEDLYLFDQSELISQFGKHGYVLYQRVRGIDNRAVEPERERKSIGKEHTFIQ
                     FLSDEDQVRVELKQIAQAVHVALHNSKLHGRTMVLKIRYADFDTLTRRKTRVDHFSQA
                     EDIFQHAWELWLDHGTIERHVRLLGITITQLDPIHYENIQLPLWNTKHL"
     gene            1073..1366
                     /locus_tag="PLT73_08645"
                     /pseudo
     CDS             1073..1366
                     /locus_tag="PLT73_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
     gene            1412..>1814
                     /locus_tag="PLT73_08650"
     CDS             1412..>1814
                     /locus_tag="PLT73_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM20476.1"
                     /translation="MEKKPESIFINRELSWLDFDSRVLALAKEKTVPLGERIKFAAIF
                     GSNMDEFFMVRVGSLYDQTLLKNNKTDNVTHMTAAEQIAAITPRVAELQAKCDKYFQH
                     LVSALAQEGYKKVDFAKLAKPQEHFWKTYFQR"
BASE COUNT          486 a          432 c          462 g          424 t
ORIGIN      
        1 gattgaagaa agggaccacc cggaacatcg cggtaagccg attgtcatag cgaatcaccc
       61 aagagaaacc ggtcgcaaag gggtagtgac cacggctaac tatatcgcca ggaaatttgg
      121 aatccattcg gctatgagtg cgcaaaaagc atttgaactc tgccctgaag cgatctttat
      181 ttctccgcga atgaatcact ataaaatgat ttcccgacag atcagaggca tatttgcgcg
      241 ctataccgat ttgattgaac ctttgtcatt ggatgaagcc tatctggatg tcacccagaa
      301 taagcagaat ctgtcgagtg cgactgtgat tgcccgaaga attcaaaggg aagtctggca
      361 ggaaaccggt cttacttgct cggcaggggt nnnnnnnnnn caaagattgc ttccgattat
      421 cagaaaccgg ctggcttgac tgtgatcaca ccggacgagg ccgcccgttt tttatgggag
      481 ttgcccatcg aaaagtttta tggcgtcggc aagaagaccg tcgaaaagat gaaactgttg
      541 ggtattcatg ccggagagga tctttatctt tttgatcaaa gtgaattgat ttcacaattc
      601 ggcaagcatg gatatgtcct ctatcaacgg gtcagaggca tcgacaaccg agccgtggag
      661 cccgaaaggg aaaggaaatc gatcgggaaa gaacatactt ttatacagtt cctgtctgat
      721 gaggatcagg tcagggtgga gctgaaacaa atcgctcagg ctgtccatgt tgcgctgcat
      781 aacagcaagc tgcacggtcg gacgatggta ttgaaaatcc gctacgccga tttcgatacc
      841 ctaacgcgca gaaaaactcg ggtcgatcat ttttcccaag ctgaggatat ttttcaacac
      901 gcctgggaac tttggctgga ccatggcacc atagaacggc acgtacgttt attggggatc
      961 accatcactc agcttgatcc tatccattac gagaacatcc agttgccgtt atggaataca
     1021 aagcacttat agcggaaagt agttattaca ttataaaggg gagataaaaa ccatgttaga
     1081 gtcattcagt caattaaatc caatcatgca agcgttcata gcaggcttgt tcacttgggg
     1141 gtgtaccatc atcggcgcct catttgtatt ttttttcaaa tctgttaacc gaaaagtcct
     1201 tgatgtcatg agcggtttcg ctgcaggagt catgatcgca gcatcgttct ggtcattatt
     1261 ggccccatcc atctctcatg cagaagagaa tggctacgga gtttggtcat gggtcccggc
     1321 atcaatcgga ttccttttgg ggggcatatc cctgcgcatg acagaaagct gggcgggcct
     1381 ggccgctgcc aatcattagg aggagataca cttggaaaaa aagccggaaa gcatcttcat
     1441 caaccgtgaa ctcagctggc ttgacttcga cagccgcgtg ctggcccttg ccaaggaaaa
     1501 gaccgtgccg ctgggcgaac gcatcaagtt tgctgctatc ttcggcagca atatggacga
     1561 gttcttcatg gtccgcgtcg gctcgctcta cgaccagacg ctgctcaaaa acaataagac
     1621 cgacaacgtc acccacatga cggcggcgga gcagatcgct gccatcacgc cgcgcgtggc
     1681 ggaattgcag gcaaaatgcg ataaatactt ccagcacctt gtctcggcgc tggcgcagga
     1741 gggctacaaa aaggtagact tcgccaagct cgccaagccg caggagcatt tctggaaaac
     1801 ctacttccag cgcg
//
LOCUS       DAOMUJ010000394         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18264_length_1810_cov_3.119658, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000394 DAOMUJ010000000
VERSION     DAOMUJ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_1810_cov_3.119658"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1327
                     /locus_tag="PLT73_08655"
     CDS             350..1327
                     /locus_tag="PLT73_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020281290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HRM20477.1"
                     /translation="MKYRNLGKSGLRVSEIALGSWLTYGKSVADQTAEACIRTAYDAG
                     INFFDTANVYEKGAAETVLGKVLKDYRRSSLVVASKVYFPMGDGPNDRGLSRKHIIEA
                     CDASLKRLDMDYLDLYQCHRYDPSVPMEETLWALDDLQRQGKILYAGVSEWPAAKIQE
                     AHQIAKQHNFRPLISNQPIYNMIERYIEVDVLPVSVANGMGQVVFSPLAQGILTGKYR
                     SGQLVPEGSRAANSKLNSSMQKYLENESLLEAIGEIEAIAHELEITMPQLALAWILRQ
                     PGVSSAIIGASRPEQIEDNIKSVDIELTDELLLGINQILLKVSSFKPRS"
     gene            complement(1615..>1810)
                     /locus_tag="PLT73_08660"
     CDS             complement(1615..>1810)
                     /locus_tag="PLT73_08660"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010765045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRM20478.1"
                     /translation="LEMKKTKADRPCITMSMGKYGVISRLAGELFGSCLTFAAAKDVS
                     APGQVSVRDVRSILEILALD"
BASE COUNT          452 a          468 c          444 g          446 t
ORIGIN      
        1 tttttgcagg atcttcacct aagtaccatg ttccttgccc catttgcggc atgatgctgc
       61 catccggtaa cgccaccatc cgattggcat tcgctatccg tagcgaagcc ttgatgtttt
      121 cgtctatgtt cgtcatttat agaccccatc ctttctgttc tttgacaccg ttccttacct
      181 caagtatacc tttttttgtc gttaatacaa aaagaatcgc agcgcttcca ttttttcgat
      241 ttgttggcct tttcctttgg cgatttcgat tttgtggctt atgataagga cagaaagcta
      301 tttcggctat catcaaagtc tgattaaatg aatcaaggag gacgagtgga tgaaatacag
      361 gaatctaggg aaaagcggtt tgcgtgtcag cgaaatcgca ttgggcagtt ggttgaccta
      421 cggaaaatca gtagcggatc agacagcgga ggcatgcatc cgaaccgctt atgatgccgg
      481 catcaacttt ttcgacacag ccaacgttta tgaaaagggt gctgctgaga ccgtgctcgg
      541 aaaagtgctg aaggactatc ggcgctccag tttggtcgtc gccagcaaag tttatttccc
      601 gatgggggat ggtccgaatg accggggctt gtcacgcaaa cacatcatcg aagcctgcga
      661 cgccagcctg aagcgtctgg acatggatta tctggatctc taccaatgcc accgttacga
      721 tccgagcgta ccgatggagg aaacactctg ggcgctcgat gatctgcagc gtcaaggcaa
      781 gattctttat gccggagtca gcgaatggcc ggccgccaaa atccaggagg ctcaccagat
      841 tgcaaaacag cataatttcc ggccgctcat ttccaaccaa ccgatctaca atatgattga
      901 gcgttacatc gaagtcgatg tccttcccgt ttccgtcgct aacgggatgg gccaagtcgt
      961 tttttctcca ttggcacaag gtatcctgac cggcaaatac agatccggcc aactagttcc
     1021 ggaaggcagc cgtgccgcca acagcaaact gaacagttca atgcaaaagt atctggaaaa
     1081 cgagtccctg ttggaagcca tcggagaaat cgaagccatc gcgcatgaat tggaaataac
     1141 catgccgcaa ctggctctag cctggattct gcgccaaccc ggcgtcagct cagccatcat
     1201 cggcgccagc cgtccagagc agatcgaaga taacatcaag tcggtggata tcgaattgac
     1261 ggatgaattg ctgcttggca tcaaccaaat tttgttgaaa gtaagcagct ttaagccgag
     1321 aagctgagca tacgatgaat cattccggac atcaaaaaag ccatccttat atccagggtg
     1381 gctttttttg cctgttctcc atccatttgt tctccaaatc gaagaaaaac gtgtgacctc
     1441 cggcgaacgg tggcttcgca ggaggtcgcc tacattttcg gttacgttct ccgatgaggg
     1501 aggctccgca ggatttgcgt ttgctgacga tcagtttact ccggcaagcg acgcttcacc
     1561 ggagctgagc ggaaatccga aagttccgaa ccgatctgtt ccgcccgcac gttcttaatc
     1621 cagcgccaga atttccagga tggaacggac atcgcggaca gatacctgac cgggtgcaga
     1681 aacatctttg gccgccgcaa aagtcaggca ggaaccgaac agttctccgg ccaagcgcga
     1741 aatgacgccg tatttgccca tcgacatcgt gatgcacggg cgatcagctt tcgttttttt
     1801 catctccaac
//
LOCUS       DAOMUJ010000395         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18266_length_1810_cov_2.071795, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000395 DAOMUJ010000000
VERSION     DAOMUJ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18266_length_1810_cov_2.071795"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLT73_08665"
     CDS             complement(<1..351)
                     /locus_tag="PLT73_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM20479.1"
                     /translation="MQNKPFKEILKAGWARFNEKRKNLWRKYRINKFIILFLLIGTLI
                     SSTYLVFLAKTANVENLKAGLEQTTVIYDRYGNEAGELYSQKGTFVTIDQISPNIQTA
                     VVSTEDKRFYKHTGV"
     gene            827..>1810
                     /locus_tag="PLT73_08670"
     CDS             827..>1810
                     /locus_tag="PLT73_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM20480.1"
                     /translation="MSITTGLLLFFLILFIASFFVMSEYVLVRIRPSRLDFLVEEGNP
                     QAKLLKNMTVKLDSYLSATQLGVTITSLALGWLGDPTFKRLFDTLFGNLPIPDAAATI
                     LSFVVSFSVLTSIQVIIGELVPKNVALSRTEKMGLRIARPLNIWYRVMFPLIFVLNKT
                     ANGISKAIGMKTIGESDDNVSEEELRLIMSQSLKSGEINREEYQFVENVFNFDERMAR
                     EIMVPRTEIVAVDFDMSLSDIANVVQQEKYTRYPVMRDDKDSIVGVINTKEVFAAFVE
                     AVQNKDESKFTVTDFVRPVITVIETIPIKELLVKMQKEHNQVAILIDEYGGT"
BASE COUNT          488 a          410 c          396 g          516 t
ORIGIN      
        1 aacaccggtg tgcttataga accgtttgtc ctccgttgaa acgacggcag tctggatatt
       61 cggagaaatc tggtcgatcg tcacgaacgt tcctttctgc gaatagagtt ccccggcttc
      121 attgccgtag cgatcgtaga tgacagttgt ctgttccaga cctgccttga gattttcgac
      181 attggcggtc ttagccaaaa atactagata agtgctgctg atcaatgtcc cgatcaacaa
      241 aaacaagatg ataaacttgt tgatccggta tttccgccag aggttcttgc gtttctcatt
      301 gaaacgggcc catccggctt tcaaaatttc tttaaatggt ttattttgca ttttttcatc
      361 cttcttcatt taatacatcc ccccagcttg ctgaatgcct ttgcggtgcg cgtggcagtg
      421 gagacacaac ctaagctttt cacatgaaag cacattgcaa caacagattc cgtatcatag
      481 taacaggaat cgctcaaata agaaaccgac ttaagtcgca gaaatgctct cgtattgcaa
      541 tttctctatt ttgatttata attttagcat aagtatcgtt ttgaattaac cgaattttgc
      601 ttaaataatt attaaggtag gtggcaccag tgggacagaa cgcatcagat gacaaccatc
      661 tgtagaccaa aagaattttc gaaaacgcgt attttcaggg attcttttgg tcgctttcat
      721 ttgtttcagc gtgaaaccag agatctcgtg aagaaatttc ctattcagga tcattatttc
      781 acaggaggtg aagtctgctc tctataaagg aagcgggcaa tcaattatgt caattacaac
      841 cggtttacta ttatttttcc tcattctttt cattgcttct tttttcgtta tgtccgaata
      901 tgtgctcgtg aggatccgcc cttcccggct ggatttcctg gttgaagaag gaaatccgca
      961 agcaaaatta ttaaaaaata tgaccgttaa actcgacagc tatctttcag cgacgcaact
     1021 gggggttacg atcacatctc ttgcccttgg ttggttaggg gatcctacct ttaagcgcct
     1081 gttcgatacg cttttcggta acttgccgat ccccgatgcg gccgcaacca tcctgtcctt
     1141 tgttgtttca ttctctgtct tgacttccat ccaagtcatc atcggtgaac tcgttcccaa
     1201 aaacgtagcc ttgagccgca cggagaaaat gggccttcgt atcgcaagac cgttgaatat
     1261 ttggtaccgc gtgatgtttc ccttgatttt tgtgctgaat aaaacggcta acggcatttc
     1321 caaagcgatc gggatgaaaa cgatcggcga atcggacgac aatgtgtccg aagaagaact
     1381 gcgcttgatc atgagtcaaa gcttgaagag cggtgaaatc aatcgtgaag agtatcaatt
     1441 cgtggaaaat gttttcaact tcgatgagcg gatggcacgt gaaatcatgg ttccccggac
     1501 ggaaatcgta gcggtggact tcgatatgtc acttagcgat atcgcaaatg tggtccaaca
     1561 ggaaaaatat acccgctatc cggtcatgcg cgatgacaag gattcgatcg taggcgtcat
     1621 caacacgaaa gaagtctttg ccgcctttgt cgaagccgtc cagaataaag acgaaagcaa
     1681 attcaccgtc accgacttcg ttcgtccggt catcacggta atcgagacga ttccgatcaa
     1741 ggaacttctg gtcaaaatgc aaaaggaaca caaccaagtc gccattctga tcgacgagta
     1801 cggcggcaca
//
LOCUS       DAOMUJ010000396         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18281_length_1809_cov_2.082668, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000396 DAOMUJ010000000
VERSION     DAOMUJ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18281_length_1809_cov_2.082668"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="upp"
                     /locus_tag="PLT73_08675"
     CDS             complement(<1..514)
                     /gene="upp"
                     /locus_tag="PLT73_08675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRM20481.1"
                     /translation="MGEFHVMNHPLIQHKLTIIREKECGTKVFREVVSEIAMLMAYEV
                     TRDMPLEDIEIETPLVKTTQKTLSGKKVAIIPILRAGLGMVDGFLAMLPAAKVGHIGM
                     YRDHETLEPVEYFVKLPTDIQERQLFIVDPMLATGGSAIAAIEALEKRGASPSAMKFI
                     CLVAAPEGVDA"
     gene            complement(645..>1809)
                     /gene="glyA"
                     /locus_tag="PLT73_08680"
     CDS             complement(645..>1809)
                     /gene="glyA"
                     /locus_tag="PLT73_08680"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRM20482.1"
                     /translation="ENFVSEDVMRAQGSILTNKYAEGYPGRRYYGGCEFVDVIENLAI
                     DRIKAIFGAEYANVQPHSGSQANMAAYRVLVQPGDKILGMDLSHGGHLTHGASVNFSG
                     QTYEFVAYGVEKESETINYDTVREIALKEQPKLIVAGASAYPRAIDFKRFREIADEVG
                     AYLMVDMAHIAGLCATGHHQNPVEYAHIVTTTTHKTLRGPRGGVILAKEEFGKKLNSA
                     VFPGIQGGPLEHVVAAKAVSFFEAATPEYTEYIGQVIKNAQAMADVFNASDLRVISGG
                     TDNHLLLLDVTSSGLNGKQVEKLLDTVEITVNKNTIPFETLGANKASGIRIGTPAITT
                     RGLKEAEARKIAELIVETIQNHTDEAKLDEIRAAVHAITKAFPLHKKPVSTEA"
BASE COUNT          454 a          436 c          380 g          539 t
ORIGIN      
        1 acgcgtccac tccttcagga gctgccacaa gacaaatgaa tttcatggct gatggcgatg
       61 cgcctctctt ttccaaagct tcaatagctg cgatagctga tccgcctgta gccagcattg
      121 gatcaacgat gaataattga cgttcctgga tatcagtcgg caatttgacg aaatactcaa
      181 ccggttccaa ggtttcgtgg tcacggtaca ttccgatatg accaactttt gctgccggca
      241 acatcgccaa gaaaccatcg accatcccta atcctgcacg caaaattgga atgatggcaa
      301 cttttttacc tgacaatgtt ttttgagtcg tttttaccaa tggcgtttcg atctcgatgt
      361 cttccaaagg catgtccctt gttacttcat aagccatcaa catggcgatt tcacttacta
      421 cttcacgaaa tacttttgtt ccgcactctt tctcgcggat aattgttaat ttgtgttgaa
      481 tcaacggatg attcattaca tggaattctc ccatatactt ttgccccact ttcgtttttt
      541 tgctcagtct attgtaattg aaaaccgata caaataccag aaataaaaca atgagaaaaa
      601 cctaaaagag ctgcctcatt gggagacagc tcttaataat ctatttaagc ttctgttgaa
      661 actggttttt tgtgcaatgg gaacgctttt gtgatggcat ggaccgccgc acggatttca
      721 tccaattttg cttcatccgt gtggttttgg atcgtttcca caatcaattc agcaatctta
      781 cgagcttccg cttccttaag gcctctagtt gtgatggcag gagtgccgat acggatgccg
      841 ctggctttgt ttgcgcccag cgtttcaaaa ggaatcgtat ttttattgac agtgatttca
      901 acggtatcca acagtttttc tacttgtttt ccgttcaaac cgctgctggt tacatccaac
      961 agcaacaagt ggttatcggt tccgccgctg atgacgcgca agtcggatgc attaaaaaca
     1021 tcagccattg cttgggcatt tttgatgact tgcccgatgt attcagtgta ctctggtgtt
     1081 gcagcttcaa agaaggatac cgctttagca gcgacaacgt gctcaagagg tccgccttgg
     1141 attccaggga agacagcact gttcaacttt ttgccgaatt cttcttttgc caagatgacg
     1201 cctccacgag gaccgcgaag tgttttatgt gtcgtcgtag ttacgatgtg ggcatattca
     1261 accggatttt ggtgatgtcc tgttgcacaa agtcctgcga tatgagccat atccaccatc
     1321 agataagcac cgacttcatc ggcgatttct ctaaaacgtt tgaaatcgat tgcgcgcgga
     1381 taagcgctgg caccggcaac aatcaatttt ggttgttctt tcaaagcgat ctctctgact
     1441 gtgtcatagt tgattgtttc ggattctttt tctacgccgt aagccacgaa ctcataagtc
     1501 tgaccgctga aattcacaga cgcaccatga gtcaaatgtc ccccgtgact caagtccatc
     1561 cccaaaatct tatcgccggg ctggaccaaa acacgataag cagccatgtt cgcttgtgaa
     1621 ccggaatgtg gttgtacatt ggcatattcc gcaccgaaga ttgctttgat gcgatcgatg
     1681 gccaagtttt cgatgacatc gacaaattcg caaccgccat aataacgacg accagggtag
     1741 ccttcagcat atttgtttgt caagatgctg ccttgcgctc tcataacatc ttcagataca
     1801 aagttttcg
//
LOCUS       DAOMUJ010000397         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18385_length_1800_cov_1.939255, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000397 DAOMUJ010000000
VERSION     DAOMUJ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18385_length_1800_cov_1.939255"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="aspS"
                     /locus_tag="PLT73_08685"
     CDS             complement(<1..1318)
                     /gene="aspS"
                     /locus_tag="PLT73_08685"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM20483.1"
                     /translation="MERRTEYCGLLTEASVGQTVVANGWVNKRRDLGGLIFIHLRDRE
                     GIVQVVFNTEFSEEAFRIAETIRGEYVLQVKGKVVLREENQINPNIGTGTIEIEAESV
                     EILAKAKTPPFYIEGDLNVSDELRMKYRYIDLRRDKMMNNMKIRHQTTRTVRNYLDDL
                     NFMDIETPYLTKSTPEGARDYIVPSRVHQGEFYALPQSPQLFKQMLMGAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETSFLSAEEIQEITENMLQKVMKDVLGKDIPAPFPRI
                     SYDEAMNRFGSDKPDTRFALELIDMNEFAKTSSFNVFKATSESGGQVRGLNVKGKADA
                     YSRKDMDNLIEYAKQYGAKGMAWMKVEEDGLKGAVAKFFTEDSDELIRLMDAEPGDLL
                     VFVADKKEIVYQALGELRLKFGRDLDLIDKSQFAFVWVVDWPLLEYD"
     gene            complement(1343..>1800)
                     /locus_tag="PLT73_08690"
     CDS             complement(1343..>1800)
                     /locus_tag="PLT73_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRM20484.1"
                     /translation="DGLVQEIGGPEVPGFGFGMGLERLILLIKDQQVDVPKLSELDVY
                     VVGLGEATNIETLKLVQNARQAGYSADRDFHDRKAKAQFKTATKNGAKVVLTIGENEL
                     NNKQVQFKVMSTGKEQTLSLAEIYADFDKVYKTQTADMTAFNEFFGKED"
BASE COUNT          433 a          460 c          357 g          550 t
ORIGIN      
        1 catcgtactc aagcaatggc caatccacaa cccaaacgaa agcgaattgg gatttgtcga
       61 tcagatcaag atcgcggcca aacttcagac gcaactctcc aagcgcttga tagacgattt
      121 cttttttatc agcaacaaac acaagcaagt ctccaggctc agcatccatc agtctgatca
      181 attcatcgct gtcttcggta aagaatttcg ccacggcgcc tttcaatcca tcttcttcga
      241 ctttcatcca agccatgcct tttgcgccgt attgtttggc gtactcgatc aggttatcca
      301 tgtctttgcg tgaataagca tcggctttac ctttaacgtt caagccacgg acttgtccgc
      361 cgctttcact tgttgctttg aatacgttga atgaagaagt tttcgcaaat tcgttcatgt
      421 cgatcagttc caacgcgaaa cgagtatccg gcttatcact gccgaaacgg ttcatcgcct
      481 catcatagga gatgcgaggg aatggagccg gaatatcttt gcccagaacg tccttcatga
      541 ctttttgaag catgttttcg gtaatttcct gaatctcctc agcagaaagg aaacttgttt
      601 cgatatccac ttgcgtaaat tctggctgtc tgtcaccgcg caaatcttca tcgcggaagc
      661 aacgaacgat ctggtaatag cggtcaaatc cagcgcccat cagcatctgt ttgaacagtt
      721 gcggcgattg tggcagtgcg taaaattcgc cttggtggac acgggatgga acgatatagt
      781 cacgcgcacc ttccggagtc gatttcgtca aatatggtgt ttcgatatcc ataaaattca
      841 agtcatccaa ataattacgg acagtacgcg tcgtttgatg gcgaattttc atgttgttca
      901 tcattttgtc tcttcttaag tcaatgtagc ggtatttcat gcgcagttca tccgatacgt
      961 tcagatcgcc ttcgatatag aacggcggtg ttttcgcttt cgccaaaatt tccacggatt
     1021 ccgcttcaat ttcgatcgtt cctgtaccga tattcggatt aatttgattt tcttcacgta
     1081 ggactacttt acctttgact tgcaggacat attctccacg gatcgtttct gcaatacgga
     1141 aggcttcttc gctgaattcc gtgttgaaaa cgacttggac gatgccttca cggtcacgca
     1201 gatggataaa gatcaatcca cccaagtccc ttcttttgtt tacccaacca ttggcgacga
     1261 cagtctgccc aaccgatgct tctgtcaata atccgcaata ctctgttctt ctttccattt
     1321 tcactaccct cttctgtatt ttctagtctt cttttccaaa aaattcatta aaagccgtca
     1381 tatcggctgt ctgtgttttg tacactttat cgaaatcagc ataaatttca gccaatgata
     1441 aagtctgttc tttgccggtt gacatcactt tgaattgcac ctgtttgtta ttcaattcat
     1501 tctcgccgat tgtcaggacc actttggctc catttttcgt agcggttttg aattgggctt
     1561 tggctttgcg gtcatggaag tctcgatccg ccgaataccc tgcttggcgc gcattttgga
     1621 ccaatttcaa ggtttcgatg ttcgtagctt ctcccagacc gaccacataa acatccaact
     1681 cggataattt tggcacatcg acttgctggt ccttgatcaa aaggatcagc ctttccaagc
     1741 ccattccgaa gccaaatcca ggaacctccg gcccgccgat ttcctgaacc aagccatcat
//
LOCUS       DAOMUJ010000398         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18483_length_1792_cov_3.063328, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000398 DAOMUJ010000000
VERSION     DAOMUJ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18483_length_1792_cov_3.063328"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLT73_08695"
     CDS             <1..919
                     /locus_tag="PLT73_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM20485.1"
                     /translation="IKAESRDRRVLTMELRVLNYFLTVVYEENITSAAEKLHITQPTL
                     SRQLMQLEEELGVSLFRRGKKKITLTSEGLLLKRRAEEILDLTSRTELELKEQKTLMN
                     GEIAIGGGETQSMRVLADFIKTFSTEYPQVTYKLYSADADDIKDRVNKGLLDICLLTE
                     PVDVDKYDFIRIPQKERWGVIMRKDSPLAQERSVAPEDLLAYPILIPGRALVQNELDN
                     WFGDAYGAVHIIGRYNLIYNAAILVEAGVGVALCLEKLVPVSEETELCFRTLAPALET
                     GVVIAWKKNQIFPPAVAKFIELLKNAFRA"
     gene            1032..>1792
                     /locus_tag="PLT73_08700"
     CDS             1032..>1792
                     /locus_tag="PLT73_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20486.1"
                     /translation="MDMRTLKKLALLSSSFVVVSGGAIAVNVPAIARDFPEVPLPLVE
                     SLTTMPSLFLIVSVLLSHAIARKVGYKRTVLVGLATVLVSGLVPVFVHHFWIIFLSRA
                     LFGFGIGLFNSLLVSFIRHFYEGAERAAMIGLQSAFEGIGGMTISFLVGQLLKIDWQT
                     SFWVYLAALPAMLLFAALVPDIPAEPAAVSSQPQQTDPLSTRSNAMDKAVFGYLTLLV
                     VAVMFYMTITVRVTPLMLANGYGDATNGSYILSLIG"
BASE COUNT          400 a          416 c          514 g          462 t
ORIGIN      
        1 gattaaagcc gaatcaaggg ataggagagt attgactatg gagttgcggg ttctgaatta
       61 ttttttgaca gtggtttatg aagagaacat cactagtgcc gctgaaaagt tgcacatcac
      121 ccagccgacc ttgtcgcgcc aattgatgca gttggaagag gaattaggcg tttctttgtt
      181 ccggcgcggc aaaaagaaaa tcacgttgac cagcgaaggg ttgctgctga aaagacgggc
      241 ggaggaaatc ctcgatctga ccagcaggac cgaactggag ctgaaggagc agaaaacgct
      301 catgaacggc gaaattgcca tcggtggggg agaaacgcag tccatgcgcg ttttggctga
      361 tttcatcaag accttcagca cggaatatcc gcaagtgacc tacaaattgt acagtgcaga
      421 tgccgatgac atcaaggatc gggtcaacaa agggctgttg gacatctgcc tgctgacgga
      481 gccggtcgat gtcgataaat atgatttcat ccggattccc cagaaagaac gctggggtgt
      541 catcatgcgg aaggacagtc cgctggcgca ggagcgctcc gttgcacctg aggatctgtt
      601 ggcttatccg atcctgattc cggggagagc attggtccag aacgaacttg acaattggtt
      661 cggcgacgcc tatggggccg tgcatatcat tgggcgctac aatctgatct acaatgcagc
      721 cattctggtg gaagcgggcg tcggggttgc gttgtgcttg gaaaaacttg tcccggtcag
      781 tgaggaaacc gagctctgct tccggacctt ggcgcctgca ttggagacag gcgtggtcat
      841 cgcctggaaa aagaatcaga ttttcccgcc ggcagtggct aaattcatcg agcttcttaa
      901 gaatgccttc agggcatgat caatcatgca aaaaaactat tatctttttc tacggttgcg
      961 ttttaaactg gtattaagaa aaatgatgta tgcattatag gcatggatat tcggaaagga
     1021 agatcatcag tatggatatg aggacactca agaagcttgc gctgttgtcc agctcatttg
     1081 ttgttgtttc cggtggagcc atcgcggtga atgtgcccgc catcgcccgg gattttccgg
     1141 aagtcccgtt gccgttggtg gaatctttga caacgatgcc ttccctgttc ttgatcgttt
     1201 cggttctgct cagccacgcc atcgcaagga aagtcggcta taagaggact gttcttgtgg
     1261 ggctggctac ggttctggtt tcaggcttag ttccggtatt tgtgcatcat ttctggatca
     1321 ttttcctttc cagggcactc ttcggattcg ggatcggact tttcaattcg ctcctggtca
     1381 gttttatccg gcatttttat gagggggccg agagagcggc gatgatcggg ctgcagagcg
     1441 cctttgaagg catcggcggg atgaccattt ccttccttgt ggggcagctg ctgaaaattg
     1501 actggcaaac ttccttttgg gtctacttgg cggctctgcc ggcgatgctt ctgttcgcgg
     1561 ccttggttcc ggatatcccg gctgaaccgg ctgccgtttc cagtcagcca cagcaaacgg
     1621 atcctttgag cacacgcagc aatgcgatgg acaaggcggt tttcggctat ctgaccctgc
     1681 tcgttgtggc ggtgatgttc tatatgacga tcacggtccg ggtcacgccg ctcatgctgg
     1741 cgaacggtta cggcgatgct acgaacggaa gctacatcct ttccctgatt gg
//
LOCUS       DAOMUJ010000399         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18613_length_1783_cov_2.250000, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000399 DAOMUJ010000000
VERSION     DAOMUJ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18613_length_1783_cov_2.250000"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..845
                     /locus_tag="PLT73_08705"
     CDS             141..845
                     /locus_tag="PLT73_08705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM20487.1"
                     /translation="MQTLIIVSHPTLADSNLQRFLWESLPAEGVTWHHLESVYPDGQV
                     DREAEQQQLLQYDRIIFQFPFYWYSSPALLKQWQDVVLTDDFAYGTDGGRLSGKEFGL
                     VLALGVNEREYQAGGREGFTISELTRPYQALAKKCGMVYLPTLTVSKFDYLNDSKKKE
                     LLIAYQQYLTKDNDASLKGSENWFKRQLQSLGQVGLSEDDQQLVEHLLAILEDNREQL
                     DDLAWTLAQMEGNQFG"
     gene            866..1048
                     /locus_tag="PLT73_08710"
     CDS             866..1048
                     /locus_tag="PLT73_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20488.1"
                     /translation="METENWVQEQLNHLMAASKDYRQKALFQETKKLFQEQYQRIEQM
                     EGELDGRIWSPKEWSD"
     gene            complement(1327..>1783)
                     /locus_tag="PLT73_08715"
     CDS             complement(1327..>1783)
                     /locus_tag="PLT73_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM20489.1"
                     /translation="ELSRLADTIWQEYYLPILGPEQVTYMLENIQSKANMEADIETGK
                     LDYFLIKSEGQSAGYLAIQLQDDKLFISKLYLLKEARGRGYAYQIMQKMVDLAIKEQK
                     KVLELTVNKYNEGSIAFYEKYGFVRTESIVSPIGGGFVMDDYVYQLPIK"
BASE COUNT          454 a          391 c          475 g          463 t
ORIGIN      
        1 cagatttcct aaatttcttt cagggctgaa cgaacccgtt cagcttttct tatttttcgg
       61 atgggtgagc atgcgacttg aactatggcc gacagcttgt tatgatgcgg aagaagaatt
      121 attgggaatg aggtgaaagg atgcaaacat tgatcatcgt cagccatccg acgctggctg
      181 attcgaatct gcaaaggttt ctgtgggaaa gtcttccggc tgaaggagtg acgtggcatc
      241 atctcgagtc ggtttatccg gatggacagg tcgatcggga agccgaacaa cagcaattgc
      301 tccagtatga ccgcatcatc ttccaatttc cgttctattg gtacagctcg ccagctctat
      361 tgaagcaatg gcaggatgtc gttttgacag atgactttgc ctacggaact gacggaggca
      421 ggttgtcggg aaaagaattc ggcctcgttt tggcactcgg tgtgaatgaa cgcgaatacc
      481 aagccggagg tagggaaggc ttcacgattt ccgaactgac ccgtccttac caggcgttgg
      541 cgaaaaaatg cggcatggtg tacctgccta cgctcaccgt ttctaaattc gattacctga
      601 acgacagcaa gaagaaagag ttgctgatcg cttaccagca ataccttacc aaagacaatg
      661 atgccagcct caagggttca gaaaactggt tcaaacgcca actgcagtcg cttgggcaag
      721 tgggactttc cgaagatgac cagcaactgg tggagcatct gcttgccatt ttggaggaca
      781 accgtgagca attggatgac ttggcgtgga cattggcaca gatggaaggc aatcagtttg
      841 gataaaagta aagcggggga cgaaaatgga gactgaaaat tgggtgcagg aacaactgaa
      901 tcacttaatg gcagcaagta aagattaccg gcaaaaagca cttttccagg aaacgaagaa
      961 gcttttccaa gagcagtatc aacggatcga acaaatggag ggcgaattgg acgggcggat
     1021 ctggagcccg aaagagtggt cggattagat tgcggcatcg acttgacctt gcttcgactg
     1081 tcagctccgg gaaacggggg cttcaccgga gttcagacga tattctgagt tattctccgg
     1141 caagcgtagc ttcgtcgtag ttgggggtgg aagttatcga tgtactccgg cgagtagaag
     1201 tctggacgga gctgcatacg catattccgt ttcgctcatc cggtcagcgg cagctggccg
     1261 cgctctttag ggataaaaaa gatagacact tcaccttttt atgggtagtg tctatctttt
     1321 ttgtttttat ttgattggca attggtagac gtaatcgtcc atcacgaagc cgcctccgat
     1381 aggggagacg atggattccg ttcgcacaaa accgtatttt tcgtagaatg cgatcgaacc
     1441 ttcgttgtat ttgttgacgg tgagctccag aacttttttc tgttccttga tagccaaatc
     1501 gaccattttt tgcatgattt gataggcgta gcctcgccca cgggcttcct tcagcaaata
     1561 aagtttactg atgaacagct tgtcgtcctg cagttggata gccaggtaac cggcggattg
     1621 cccctcgctt ttgatgagga aataatccaa ttttcctgtt tcgatatctg cttccatatt
     1681 cgctttggac tggatgttct ccaacatata ggttacttgt tccggaccta gaatcggcag
     1741 ataatactcc tgccagatgg tgtcagccaa gcgggacagc tcg
//
LOCUS       DAOMUJ010000400         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18668_length_1779_cov_1.752320, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000400 DAOMUJ010000000
VERSION     DAOMUJ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18668_length_1779_cov_1.752320"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..857
                     /gene="gmk"
                     /locus_tag="PLT73_08720"
     CDS             246..857
                     /gene="gmk"
                     /locus_tag="PLT73_08720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRM20490.1"
                     /translation="MTEKGLLIVLSGPSGVGKGTVRQAIFSRGEREFIYSISATTRKA
                     REGEVDGKDYFFKEREEFERMIADNELLEYTEYVGNYYGTPIDYVRRTLDTGKDIFLE
                     IEVQGAMQVKERMPEGIFIFLTPPDMTELESRIVNRGTDESPIIKQRMEKAIEELSLM
                     RYYDYAVENDTVENAVQKVLGIIQSEHLKVSRILDTICADERE"
     gene            864..1079
                     /gene="rpoZ"
                     /locus_tag="PLT73_08725"
     CDS             864..1079
                     /gene="rpoZ"
                     /locus_tag="PLT73_08725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020280206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRM20491.1"
                     /translation="MMLHPSIDKLLEQVDSKYSMVILSSKRAHELHGRSEPLLDHYQS
                     YKNVGRALEEVVAGELEIDPNSIIPER"
     gene            1222..>1779
                     /locus_tag="PLT73_08730"
     CDS             1222..>1779
                     /locus_tag="PLT73_08730"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRM20492.1"
                     /translation="MQIAKVIVDIPAMQTNRPFDYAIPPAMEQKLGKGMRVEVPFNTR
                     VIQGFITEISDHTDFEGELKEIIQPLDIEPALTEELLLLGEEMAETVYAYRIHCYQTM
                     LPPLMKAKYEKEIEVVDELDEETFYGLFQGKSKMSWEDAVDRGILPQLIELKRAGKIE
                     VNYILKNQAKAKTVQVYYSDLEMGSL"
BASE COUNT          553 a          317 c          457 g          452 t
ORIGIN      
        1 cctaaatttc tttcagggct gaacgaaccc gttcagcttt tcttgtcttt ccggcgcttg
       61 tgagaggcga gcgcattcca tccaaggggt gcagttgttg gaaacggtaa ctaataaaat
      121 gttggcggaa gactccggta aaggttttaa ccttgatgaa attgtgttaa actatgaaat
      181 aataaacaga acgccgcatt aggctgtacg atgctataat aatataattg gatggtgggc
      241 attaaatgac agaaaaagga ttgttgattg tcttatccgg accttcggga gtaggcaaag
      301 ggactgtgag acaagcaatt ttttctcgtg gtgaacggga gttcatctat tcgatttccg
      361 ctacgacccg caaagcgcgt gaaggggaag tggatggcaa agattacttc ttcaaggaaa
      421 gagaagaatt cgagagaatg atcgccgata atgaactgtt ggaatatacc gagtatgtgg
      481 ggaattacta tggtacgcca atcgactatg taagaagaac attggacaca ggaaaagata
      541 tttttctgga gatcgaagtt caaggcgcca tgcaagtgaa ggaacggatg ccggaaggga
      601 tattcatctt cttgacgcct cctgatatga cagaattgga gtcacggatc gtaaatcgcg
      661 gtacggatga atcgccgatc atcaaacaaa gaatggaaaa ggctattgaa gagctcagtt
      721 tgatgcggta ttatgattat gcggtagaaa acgacacagt agagaacgcg gtccagaaag
      781 tgcttggcat catccaaagc gaacacttga aagtctcacg gattctggat accatctgtg
      841 cggatgaaag ggagtaaaat aatatgatgt tacatccttc gatcgataaa ttgttggagc
      901 aagtggattc aaaatattca atggtcattc tttccagcaa aagagcgcat gaattgcatg
      961 gtcgctcaga acctttattg gatcattacc agtcatacaa gaatgtcgga cgtgcgctgg
     1021 aagaagtagt tgccggcgag ttggaaatcg atccgaattc catcatccct gaaagataac
     1081 cgaataggaa tgaaaaaaga ctactgtttt tgaacttcaa aatcagtagt tttatttttt
     1141 tgatgccgaa aaaatccgag aagactttat cggtataatt tagtaagata gtggttagaa
     1201 aaaggaatgg gagggtttta cgtgcaaatt gcaaaagtga ttgtggatat acctgccatg
     1261 cagaccaatc gtccctttga ttatgcgatc cctcctgcta tggaacaaaa gttgggcaaa
     1321 gggatgcgcg ttgaagtgcc tttcaacact cgcgtcatcc aagggttcat aacggaaatt
     1381 tctgatcaca ccgattttga aggggaactg aaagaaatca tccaaccatt ggatatcgag
     1441 ccggcattga ccgaagagtt gctgctgttg ggcgaggaaa tggcggaaac cgtttatgcc
     1501 tatcggatcc attgttacca gacgatgttg ccgcctttga tgaaggcaaa gtacgaaaaa
     1561 gaaattgagg ttgtcgatga attggacgag gagactttct acggactttt tcaagggaaa
     1621 agcaaaatga gttgggaaga tgccgtcgac cgcggcattt tgccgcaact aatcgaattg
     1681 aagcgagccg ggaagattga agtcaattac atcctcaaaa accaggcgaa ggcaaaaacg
     1741 gtgcaggttt attattccga tctggaaatg ggcagtctg
//
LOCUS       DAOMUJ010000401         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18683_length_1778_cov_1.596634, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000401 DAOMUJ010000000
VERSION     DAOMUJ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18683_length_1778_cov_1.596634"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="ppdK"
                     /locus_tag="PLT73_08735"
     CDS             complement(<1..1727)
                     /gene="ppdK"
                     /locus_tag="PLT73_08735"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRM20493.1"
                     /translation="MTKQWVYRFSEGKKEQKMLLGGKGANLAEMTNLGLPIPPGFTIT
                     TESCIHYFDEGRTLWAALKQQIDSALIDLENAVGKSFHNPANPLLVSVRSGAAFSMPG
                     MMDTILNLGLNDASVAGLAEQTQNPTFAFDSYRRFIQMFADVVKSVPRIHFEAILDTV
                     KNENGYAFDNQLTLEDLAELVAQYKAVYQRETGETFPQEPLDQLHQAVQAVFSSWNNE
                     RAILYRDIHNISHNLGTAVTVQSMVFGNRGNDSGTGVAFTRNPATGAKKLFGEFLMNA
                     QGEDVVAGIRTPLDIDTLEQVMPAVYNEFKVIAEKLEDHYADMQDIEFTIEKGKLYLL
                     QTRNGKRTAAAAVAVAVDLADEGLITKEEAVMRIETGQLDQLLHPTFDTKALAEAPLL
                     TTGLGASPGAASGHIYFSTADAEAAQKEGLPVILVRRETSPEDLAGMMSSEGILTARG
                     GMTSHAAVVARGLGKCCVAGCTAAVIDEENKTVVIDDVTLHQGDLLSIDGATGRVYAG
                     KVAKQNPTLGGKFSTFMDWVNEMKVLQVKANADSPTDVRQALSLGAEGVGLCRTEHMF
                     FHKDRIPAVR"
BASE COUNT          348 a          481 c          510 g          439 t
ORIGIN      
        1 cggacggccg gtatccgatc cttatggaag aacatgtgct cggtccggca aagtccgaca
       61 ccttccgcgc ccagggacaa ggcctgtctg acgtcggtcg gggaatccgc attggctttg
      121 acctgcagca ctttcatttc gttgacccag tccatgaagg tgctgaattt gcctcccaaa
      181 gtcgggttct gcttggcgac tttgccggcg tagactctgc cggttgcgcc atcgatcgaa
      241 agcaggtcgc cttggtgcag ggtgacgtca tcgatgacaa ctgttttgtt ttcttcatcg
      301 atgacggctg cggtacagcc ggccacacaa catttgccca aaccgcgggc gacgactgcc
      361 gcgtgcgaag tcatgccgcc gcgcgccgtc aggatgcctt ccgaactcat catgcccgcc
      421 aaatcttcag gggatgtttc gcggcggacg aggatgaccg gcaggccctc cttttgtgcc
      481 gcttcggcat cggcagtgga gaaatagata tgtccggaag ccgctcctgg cgaagcgccg
      541 agcccggtcg tcaacaaggg agcttcagcc aaagctttcg tatcgaaagt agggtggagc
      601 aactggtcca gttggccggt ttcgatgcgc atcacggctt cctctttcgt gatcaggcct
      661 tcgtcggcca aatccacggc cactgcgacg gctgcggccg ctgtccgttt cccgttgcgt
      721 gtctgcagca agtatagttt ccctttttcg atcgtgaact cgatgtcctg catatcggcg
      781 tagtggtcct ccagtttttc ggcgatgacc ttgaattcgt tgtagacggc aggcatcact
      841 tgctccagtg tgtcgatgtc caatggcgtg cggataccgg ccacgacatc ctcgccttgc
      901 gcattcatca ggaattcgcc gaacagtttt ttcgcgccgg tcgccgggtt gcgcgtgaag
      961 gccacaccgg taccagaatc gttgccgcgg ttgccgaaca ccatgctttg gacggtgacg
     1021 gctgtgccga ggttatgcga aatgttgtga atatcgcggt acagaatagc cctttcatta
     1081 ttccatgaag agaagacagc ctggactgct tggtgcaatt gatccaacgg ttcctgcggg
     1141 aaagtttcgc cggtttcgcg ttggtagacg gctttgtatt gcgcgaccag ctcggccagg
     1201 tcttccaaag tcaattggtt gtcgaacgcg taaccgttct catttttgac tgtatccagg
     1261 atcgcttcga aatgaatgcg cggaacggat ttcacgacat cggcgaacat ctggatgaaa
     1321 cggcggtagc tgtcgaaagc gaaagtcggg ttctgcgtct gttcagccag accagcgaca
     1381 gatgcatcgt tcagccccaa attcaggatt gtgtccatca tgcccggcat cgagaaggcg
     1441 gcaccggaac ggaccgacac cagcaacggg ttggcagggt tatggaaaga ttttccgacc
     1501 gcattttcca ggtcgatgag ggcggaatcg atctgctgct tgagcgcagc ccaaagtgtt
     1561 cggccttcat cgaaataatg gatgcaggat tccgtcgtga tcgtgaatcc tggagggatg
     1621 ggcaggccga gattggtcat ctcggctagg ttggcgcctt tgccgcccaa caacatcttt
     1681 tgttcttttt tgccttctga aaaacggtaa acccattgct tcgtcatatg cttttcctcc
     1741 ttgtggctga ttataggtta tagttgcttg cggctgat
//
LOCUS       DAOMUJ010000402         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_18691_length_1777_cov_2.674797, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000402 DAOMUJ010000000
VERSION     DAOMUJ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18691_length_1777_cov_2.674797"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLT73_08740"
     CDS             <1..706
                     /locus_tag="PLT73_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20494.1"
                     /translation="KRREKMGHPMNDLSLHMVFTGNPGTGKTTIARLISQILKSIGVL
                     KRGHMIEVTREDLVGQYVGQTGPKTLEKIKEAYGGVLFIDEAYSLYSASENDFGYEAI
                     STLIKEMEDNRDKLVVIMAGYPSEMEQMLSMNSGIRSRIAYTVDFPDYDSEELTEIFH
                     MAASQQGFVLTEEAQEKVKNLFVQSYTTRDSHFGNARTARSLFEKAKLHQSNRLAEDE
                     EADLFMILPEDIEENS"
     gene            complement(758..1624)
                     /locus_tag="PLT73_08745"
     CDS             complement(758..1624)
                     /locus_tag="PLT73_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM20495.1"
                     /translation="MSRVILTTESGADIPTELAKKHTIRIVPMHVIMDDVDHLDGSFP
                     VTDLYDYYERTKKAPSTTATNPNEYTAFFEKIRSEHPESSIVHIGYTSKASSSFQSAV
                     LAAEDFENIYLIDALNVSGGLAAIVLYAADLLEKEPDIAVEKLVAAIEAVVPRMHFSF
                     MPSGLEFLRAGGRVSNTAYLGASLLKIKPTIELIDGKLVSAKKYRGKMSRVVVDFFDD
                     YLKAYAIDDNKLYLIHSLGLDEEIMTQMENYAKENGFRDVTWVQTGGVITSHGGPGAF
                     GIAGIEKSGNTE"
BASE COUNT          458 a          434 c          468 g          417 t
ORIGIN      
        1 gaaacgccgc gaaaagatgg ggcatccgat gaacgatttg tccttgcaca tggtcttcac
       61 cggcaacccc ggcacaggta agacgaccat cgcgcgattg atcagccaaa tcctgaaatc
      121 catcggtgtg ctgaagcgcg gacacatgat cgaagtcacg agggaagatt tggtcggaca
      181 gtacgtcggc cagaccggac cgaagacatt ggagaaaatc aaggaagcgt acggcggcgt
      241 cttgttcatc gatgaggcct attcccttta ttccgcttcc gaaaacgact tcggttatga
      301 agccatcagc accttgatca aggagatgga ggacaaccgc gacaaattgg tcgtcatcat
      361 ggcgggctat ccgagcgaga tggagcagat gctcagtatg aactccggca tccgcagccg
      421 tatcgcctac acggtcgatt tcccggatta cgatagtgaa gaactgacag aaattttcca
      481 catggccgcc tctcagcaag gttttgtgct gacggaggaa gcccaggaga aagtgaagaa
      541 tctgttcgtg cagtcctata caacacgcga cagccatttc ggcaacgccc gcacagccag
      601 gagcctcttc gagaaggcga agctgcacca aagcaaccgc ttggccgaag atgaggaggc
      661 cgacctgttc atgatcctgc cggaggatat cgaagagaat agttagataa aaaaacttct
      721 gcacacggaa ttattccgag ggcagaagtt ttttgtctta ttcagtattt cctgattttt
      781 cgatcccagc aatcccgaaa gcgccgggac cgccatggga agtgatgacg ccgccggtct
      841 ggacccaagt gacatcccgg aagccgtttt ccttcgcgta gttttccatt tgggtcatga
      901 tttcttcatc caaaccgagc gaatggatga ggtacagctt gttgtcgtca attgcgtaag
      961 ccttcaggta gtcatcaaaa aaatcgacaa cgacgcggct catcttgccg cggtattttt
     1021 ttgcggaaac caatttgccg tcgatcaatt caatcgtcgg ttttatcttc aggagcgatg
     1081 cgcctaaata ggctgtattg gagacgcggc cgcctgccct caggaactcg aggccggatg
     1141 gcatgaacga gaagtgcata cgcggcacta cagcttcgat ggcggcaacc agtttttcga
     1201 ctgctatatc cggctccttt tcaagcaaat cggcagcata aagcacgatc gcggccaaac
     1261 cgccggatac gttcagggca tcgatgaggt aaatgttctc gaaatcttcc gccgccaaca
     1321 cagcactctg aaatgaggag gacgccttgg aggtatagcc gatatgcacg atgctgcttt
     1381 cgggatgctc agagcggatt ttttcgaaaa aggcggtata ttcgtttggg ttggttgcgg
     1441 tcgtggaagg ggcttttttg gttcgctcgt aataatcata cagatctgtg accggaaaag
     1501 agccatcaag atgatcgaca tcatccatga tgacgtgcat cggcacaatc cggatagtgt
     1561 gtttctttgc caactcagta gggatatccg cgccgctttc agtcgtaagg attactctgc
     1621 tcatgttatt cctccgtctc caatggtcaa atatattatt gccaatggac ttaatttgtt
     1681 ggttccaata taccacactt ttagtgcccg accaaaaagt gttatgatgg catcagaacc
     1741 aggaagggag ggatgaggca tgctcaagag aggtaat
//
LOCUS       DAOMUJ010000403         1751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19070_length_1751_cov_2.599057, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000403 DAOMUJ010000000
VERSION     DAOMUJ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19070_length_1751_cov_2.599057"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1052)
                     /locus_tag="PLT73_08750"
                     /pseudo
     CDS             complement(1..1052)
                     /locus_tag="PLT73_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(1132..>1751)
                     /locus_tag="PLT73_08755"
     CDS             complement(1132..>1751)
                     /locus_tag="PLT73_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20496.1"
                     /translation="GPIGWTIGQWVSEVVYAGLTGDFRWLFGLFFGTLYSPLVITGLH
                     HMSNAIDLQLIADFGGTNLWPMIALSNIAQGSAVLAIIFLHRGNEKEEQVSIPAAISC
                     YLGVTEPAMFGINIKYIYPFVAAMIGSGIAGLVATTFNVMANSIGVGGIPGFLSIQTD
                     SMAMFIVCMAIAIVVPFLLTVLFEKKQLFAPKGEVEDAPVIQTQK"
BASE COUNT          412 a          469 c          419 g          451 t
ORIGIN      
        1 gtaaggcgtt ccccgcaaga gatggattgt ttgcgccagc atggaggctg tctcgaaagg
       61 ataccgctcc ggatcaccga atcggctgtt ggctcgcggt tgatcatgat tgttccagaa
      121 cagtgcgttc catccgtttc cgtccgacat ccctgcctgc cactcattca gaattcgctt
      181 cagttcaaga aaatcgaatg gctgatccgt ccaactttca gatggtggaa actgaaaatc
      241 atcgagagtt cgtttctgtc cggattcgaa taaagaatac cattttcgac agtggtcgag
      301 gacatctcgc cgaccgtgat gatatcctcc agcgtcccga acgatgcttg gttcaactca
      361 cggatgtact catggacgat cggtcgatcc gtgtagaggc tcttttcgtt gcttccttca
      421 ctgtctacca gcgcgacatc ttttccgatc aggttcaaca catcgaagcg gaagcccttg
      481 atgccctttt ccaaccagaa attgacgact ttctgcagtt cctcgcgcac tttcgggttg
      541 cgccagttca gatccgcttg cgtgacatca aacagatgca ggtagtactt gccggtgtcc
      601 ccgaatggcg cccacgcttc gccgccgaat ttcgaactcc agttggtcgg caggctgccg
      661 tcttctttcg gatcgcggat gatgtaataa tcctggtact ctttgtcccc ggccaacgct
      721 ttccggaacc actcatgtgt ggtcgatgta tgattgagca ccatgtcgaa cataatttcg
      781 atgccatgct gcttcagctt accgatcagt tcttcaaaat ccgccatcgt gccgaaaaca
      841 ggatcgatgg cataataatc agtgatgtcg tatccattgt ccatttgcgg ggatacgaag
      901 atcggattca tccaaatcat atcgatgcct aactccgcca aatagggcac tttgctgatg
      961 atcccttgga gatcaccgat accgttgcca tcggaatcct tgaatgattt tggataaagc
     1021 tgatagatca cttttttttg aaagtctttc attgtaaagc cccctgtctt cttactatga
     1081 ctgaaggcaa aaagcttcag tcacagcttt ttgccttcag taattcactt tttatttttg
     1141 cgtttggata accggtgcgt cctcgacttc gcctttagga gcgaaaagtt gtttcttttc
     1201 gaacaaaacc gtcaacagga acggcacgac aatcgcgatg gccatacata cgatgaacat
     1261 tgccatcgaa tcggtctgga tgctcaagaa accgggaatc ccgccgaccc cgatgctgtt
     1321 cgccatgacg ttgaaggtcg tagccaccaa cccggcgatt ccggatccga tcatcgctgc
     1381 aacgaacgga tagatgtatt tgatgttgat cccgaacatg gccggctcgg ttacgcccaa
     1441 gtagcaggag atggctgccg ggatggaaac ctgttcctct ttttcattcc ctctgtgcag
     1501 gaagatgatc gccagcactg ccgatccttg tgcgatattc gaaagggcaa tcatcggcca
     1561 caggttcgtg ccgccgaaat cggcgatcag ctgcagatcg atggcattgg acatgtgatg
     1621 aagccccgtg atgacgagcg gcgaatagag cgtaccgaag aacagaccga acagccatct
     1681 gaagtcccct gtcaatccgg catagacgac ttcggaaacc cattgtccga tcgtccaacc
     1741 gataggaccc a
//
LOCUS       DAOMUJ010000404         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19086_length_1750_cov_1.692035, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000404 DAOMUJ010000000
VERSION     DAOMUJ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19086_length_1750_cov_1.692035"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLT73_08760"
     CDS             <1..444
                     /locus_tag="PLT73_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM20497.1"
                     /translation="SEYNLFTFILLMSAFSWAGKSKVFRNRTQSETKRDYVRASKTAG
                     TNDLLIMFREILPNFGSLVIINTTMSLARNIGMETGLSFLGFGLPSTVPSLGNLLRYA
                     ISPTVFENYLWVWLPASTFIFAMLLCIIYVGQALRRAADAKQMQA"
     gene            459..>1750
                     /locus_tag="PLT73_08765"
     CDS             459..>1750
                     /locus_tag="PLT73_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM20498.1"
                     /translation="MLNKMIVSFFLAALLGGCGNENILSETDGNVSDSSVVIQEFDTV
                     MNNDDPAITGGMLKYALVAESSFQGIFNPVFSEDSYDEELMGFSHESIFSYDEDFQLT
                     QAGMGTFVVNENLKTVSITLQDNLKWSDGAVLTVDDILYAYEVIGHPDYPGSLYGEAF
                     ANVLGMTEYHEGTADAISGIEVLDDTHLTIAFKELNPSIFQAGGALWSQPLPRHQIES
                     IPVSKMAGSAQLREQPVGTGPFRVKEIVPGESVLFEANEYYWKGKPKLDHILVEVVGS
                     ATIVSEMEAGNYDVAIMPTSSYETYKDFDNITLLGREELATTYIGFKLGSRDVEAGDN
                     SLNLSSKMADAALRQAMAYALDNEFVAAVLFDGLRQPANGMIPSVFEGVSADEAVGFS
                     YDPEKAASLLDEAGYLDNDGDGFRETPEGEQLEINFAAV"
BASE COUNT          449 a          361 c          461 g          479 t
ORIGIN      
        1 tcggagtaca atctctttac gttcatttta cttatgagcg catttagttg ggcagggaaa
       61 tcaaaggttt ttcgaaaccg aacgcaatct gaaaccaaac gtgactatgt gcgggcatcc
      121 aaaacagcag ggacaaacga cttgctgatc atgttccgtg aaatcttgcc taattttggt
      181 tcacttgtca tcatcaacac gactatgtca ttggcaagga atatcgggat ggaaacaggt
      241 ttgagttttt tgggttttgg gttgccgtca accgtgccaa gccttggcaa tttgttacgt
      301 tatgcaatct ctccaaccgt ctttgagaac tacttatggg tatggttacc agcatccaca
      361 ttcatctttg caatgttgtt gtgcatcatt tatgtcggac aggcattgcg gcgtgcggcc
      421 gatgctaaac aaatgcaagc ataataagga ggatgagaat gcttaataaa atgattgtat
      481 cgttcttctt ggctgcgttg ctgggcggtt gcggaaatga gaacattcta tcggaaacgg
      541 atgggaacgt gtccgattca tccgttgtca tacaggaatt cgacactgtc atgaacaacg
      601 acgatcctgc catcaccgga ggaatgttga aatacgcgct ggttgctgaa tcttccttcc
      661 agggtatctt caacccggtt ttttctgaag attcttatga cgaggaacta atgggctttt
      721 cacatgaatc catcttttct tatgatgagg atttccagct tacccaggcc ggcatgggca
      781 cttttgttgt aaatgaaaat ttaaaaacag tgtcgatcac tttgcaggat aatctgaagt
      841 ggtctgacgg ggcggtactc acggtagatg atattcttta tgcctatgaa gtaatcgggc
      901 atccggacta tcccggttct ctttatggag aggcctttgc gaatgttctg ggcatgaccg
      961 agtatcatga aggtacggct gatgcgatca gcggaatcga agttttggat gatacgcacc
     1021 tcaccattgc ttttaaagaa ttgaatccat ccatttttca ggccggagga gccttatgga
     1081 gtcaaccgct gccgaggcac caaattgaaa gtatccctgt ttcgaaaatg gctggctccg
     1141 ctcaactgcg tgaacaacct gttgggacag gtccatttcg ggtcaaagaa atcgttccgg
     1201 gagagtcggt cctttttgaa gccaatgaat attattggaa agggaaaccg aagttggatc
     1261 atatcctcgt ggaagttgtg ggttcggcga cgattgtatc cgaaatggaa gcggggaatt
     1321 atgacgtcgc aatcatgccg acgagttctt acgaaaccta taaagatttt gataatataa
     1381 ccttgctggg cagagaagaa ttggcgacaa cctatatcgg gttcaaatta ggatcccgag
     1441 atgttgaagc cggcgacaac agcctgaatc tgagttccaa aatggcggat gcggcattgc
     1501 ggcaagcgat ggcatatgcg ctcgataacg aatttgtcgc tgcggtcctt ttcgatgggc
     1561 tgcggcaacc ggcaaacggt atgattccat ccgtattcga aggcgtcagt gctgatgagg
     1621 cggttggctt cagttatgat ccggagaagg ctgcaagtct gttggatgag gctggctatc
     1681 tggacaatga cggcgatggt ttccgggaga cgccggaggg agagcaactc gaaatcaact
     1741 ttgcggcagt
//
LOCUS       DAOMUJ010000405         1747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19125_length_1747_cov_1.834515, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000405 DAOMUJ010000000
VERSION     DAOMUJ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19125_length_1747_cov_1.834515"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLT73_08770"
     CDS             <1..489
                     /locus_tag="PLT73_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016429 - tRNA
                     (adenine-N1-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HRM20499.1"
                     /translation="VSARLGDGMDVIETTDDINVVTICGMGGDLISKILEKGRLKDKL
                     VGVERLILQPNNGEKKLREWLIGHQYKIIDETILEENGKIYEILVAEKAESPENYTDL
                     EYSFGRFLLQEKNDIFRKKWSAEIDKCQYILDSMQKASNDLNEKEQQVINKINEIKEV
                     LK"
     gene            486..1619
                     /locus_tag="PLT73_08775"
     CDS             486..1619
                     /locus_tag="PLT73_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRM20500.1"
                     /translation="MTSITGYEFIELFESHVPTWLAEDGDPVGLHLGDLSRPVRRILV
                     TLDVRPEVVQEAIEKKADFIFSHHPPIYRPLKNLDVSDKQTKMYVDLLKHDISVYAAH
                     TNLDNANNGMNDWLSEALGLLDVEIMDVTKRLPVKKISVCVPNAECNRVRLAMADAGA
                     GNISDEYSHCSFEAQGVGRFTPMEGAKPAIGHINEPEEVQEKKIEMIVEDKYLADVLE
                     ALYEAHPYEEPVYEVYTINNFQREYGLGRVGNLALPMSLRSFIQYVKDVFQIEGMRFI
                     AADLDQTISRVAVCGGDAGKYYRKAIKKGADVYITGDVYYHTAHDMQADGLTVIDPGH
                     HIEQICKPKLLELFTEWKKENEWDLEVIASEINTDPFIFDSQL"
BASE COUNT          543 a          350 c          433 g          421 t
ORIGIN      
        1 gtcagcgcta ggcttggcga tggcatggat gtaattgaga cgacagacga tatcaacgtc
       61 gtcaccatct gcgggatggg tggggacttg atttccaaaa ttctggaaaa aggcagactg
      121 aaagataaac tggtcggtgt ggaacgattg attcttcagc ccaataacgg agaaaagaaa
      181 ctgcgtgaat ggctgattgg ccatcaatac aaaatcatcg acgaaacgat attggaagaa
      241 aatggtaaga tttatgaaat cctcgttgcc gagaaggcgg agtcgccaga aaactacacc
      301 gatttagaat atagcttcgg acgtttcctg cttcaggaaa aaaatgatat tttccgcaaa
      361 aaatggtcag ccgaaatcga taaatgccaa tacatattgg acagtatgca aaaagcaagc
      421 aacgatctga atgaaaaaga acagcaagtg atcaataaaa tcaatgaaat taaagaggtg
      481 ctgaaatgac aagcattacg ggttatgaat tcatcgagtt atttgaatcg catgttccga
      541 cttggttagc agaggatggg gatccagtgg gactccattt gggagaccta agccgtccgg
      601 tccgccgtat tttggtgaca cttgatgtcc gtcccgaagt cgtccaagag gccatcgaga
      661 agaaagccga ttttatcttt tcccatcacc cgccgatcta ccgaccgttg aaaaatctgg
      721 atgtatccga caagcaaaca aaaatgtacg ttgatttgct caagcatgac atcagtgtct
      781 atgcggccca cacgaatctc gacaatgcga ataacgggat gaacgattgg ctttctgaag
      841 cattggggtt gttggacgtt gaaatcatgg atgtcacaaa gcgtttgcct gtgaagaaaa
      901 tatccgtatg tgtaccgaat gccgaatgca atcgtgtccg cttggctatg gccgatgcgg
      961 gagcgggcaa catttccgat gaatatagtc attgctcatt tgaggcgcaa ggcgttggcc
     1021 gattcactcc catggaaggt gctaagcctg ccataggcca tataaacgaa ccagaggaag
     1081 tccaagagaa aaagattgaa atgatcgtcg aagataaata cttagctgat gtcttggagg
     1141 cgctgtatga agcgcatcct tacgaagagc ccgtctacga agtatacaca atcaataatt
     1201 tccaacgcga atacggctta ggaagggtcg gtaatttggc tttgccgatg tctttgcgct
     1261 ccttcatcca gtacgtcaag gatgttttcc agatcgaggg catgcgtttc atagcagctg
     1321 atttggatca gacaatcagt cgtgttgccg tctgtggcgg ggatgccggc aaatattacc
     1381 ggaaagcaat caaaaagggt gcggatgtgt acatcactgg cgatgtctac taccacacgg
     1441 cccacgatat gcaggctgat ggactgaccg tcatagaccc aggccatcat atcgagcaga
     1501 tttgtaaacc gaaattattg gaattattca ctgaatggaa aaaagaaaat gagtgggatc
     1561 tggaagtcat cgcttccgaa atcaacacgg atccttttat tttcgatagt caattgtgaa
     1621 aggatgtcaa agcatgcaag aaaaattagt ggaccgtttc tttaaataca taaaattcga
     1681 gacaagatcg gatgaaaaaa gtctgacagt gccctcaacc caaaatcagg tggattttgc
     1741 aaaaatg
//
LOCUS       DAOMUJ010000406         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19141_length_1746_cov_2.137788, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000406 DAOMUJ010000000
VERSION     DAOMUJ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19141_length_1746_cov_2.137788"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLT73_08780"
     CDS             <1..380
                     /locus_tag="PLT73_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HRM20501.1"
                     /translation="GLYLNGFNIGSTGVNSASAEAFGLETRSVILEDFYRPEFMPSNE
                     KILMKLVYEVGTTRIVGAQLMSKYDITQSANTLSLAIQNKMTIEDLALVDFFFQPHFD
                     RPWNYLNLLAQKALEQEAELAAK"
     gene            556..>1746
                     /locus_tag="PLT73_08785"
     CDS             556..>1746
                     /locus_tag="PLT73_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM20502.1"
                     /translation="MTTDPSNQTLLWQLLLIAVLTLVNAFFAASEIAFVSLNKNRVAN
                     QAIKGDEKARKILALLDHSDDFLATIQVAITFAGFLSSASAANTFAAKLDPYLSNIPG
                     AEQAAILIVTLALSYISLVFGELYPKQLGLQRPEEVARAGAGFITVVQKLMRPFVWFL
                     SFSTSVLEKMTPITFTSGNDMFSREEVRELLEKSSDEGTIERMEFNMLKGVLSMDNKM
                     AREVMVPRTDTFMLDVNDDVEENIEAALDSPYTRIPIYEDDKDNIIGVLHLKNLLKES
                     KNTPIEQIDLRKIMNEPLFVPETILTDELMSYLKKSHNQLALLHDEYGGMVGIVTLED
                     ILEEIVGDIEDEYDESYVLIERIGDNVYEADGATPLHRFNDYFGTQLESADVDTIAGY
                     LLTEL"
BASE COUNT          500 a          348 c          413 g          485 t
ORIGIN      
        1 caggcttata cctaaacgga ttcaacattg gctcaacagg tgtgaattcc gcaagtgccg
       61 aagcattcgg tcttgaaaca cgttccgtaa ttcttgagga tttctaccgt cctgaattca
      121 tgccatcaaa cgaaaaaata ttgatgaaac ttgtctatga agtaggcaca acccgaatcg
      181 ttggcgcgca actgatgtcc aaatatgaca tcacacaatc agccaacaca ttatcattgg
      241 ctatccaaaa caaaatgacg attgaagatt tggcacttgt tgacttcttc ttccaacctc
      301 attttgaccg tccttggaac tacttgaact tgttggctca aaaggctttg gaacaagaag
      361 ctgaattagc tgctaaataa ttcaattcca taatttggaa cccctccgtg atcgcttgtt
      421 gattatcgga ggggttcttt tgttggagaa agggtatatc cggataaaat acttgttttt
      481 tttgccgggt cattagataa tgaaagataa caatcaactt tcaaagagat ctaaaaaacg
      541 aaggagaaac attctatgac cacagacccc agtaatcaga cattgctttg gcaattgttg
      601 ttgattgcgg tattgacatt ggtgaacgca ttttttgcgg catctgaaat cgcatttgtt
      661 tcattgaata aaaacagggt ggcgaaccag gcaatcaaag gtgatgaaaa agcacgcaaa
      721 attttggcgc ttttggatca ttccgatgac tttttggcga ccatccaagt tgccatcact
      781 ttcgcgggct tcctttccag tgcttcggct gccaatacat tcgcagccaa actcgatccc
      841 tacctgagta atataccagg agcagaacag gcagccatcc tgatcgtgac gttagcactc
      901 tcctacattt ccttggtatt cggtgagttg tatccgaaac aactgggact gcaacggccg
      961 gaagaggtgg cgcgggccgg tgcgggtttc atcactgtcg tacaaaaact gatgagacct
     1021 ttcgtttggt tcttgtcttt ttcaacgagt gttcttgaga aaatgacacc gatcactttc
     1081 acaagcggaa atgacatgtt ttcgcgtgaa gaagtgcgtg aattgttgga gaagagcagt
     1141 gatgaaggga ccatcgaacg gatggaattc aatatgctta aaggtgtgct ttccatggac
     1201 aataagatgg ctagggaagt aatggtaccc cggacggata ccttcatgct ggatgtgaac
     1261 gatgatgtcg aagaaaacat agaagccgcg ctggattctc cttatacacg cataccgatt
     1321 tacgaggacg acaaggataa tatcattggt gtgttgcatt tgaaaaattt attgaaggaa
     1381 tccaaaaata cgcccatcga acagattgat ttgcggaaaa tcatgaatga gccactattt
     1441 gttccagaaa ccatactcac ggatgagttg atgtcttacc tgaagaaaag ccataaccag
     1501 ttggcgttgc ttcatgacga gtatggcggt atggtcggga ttgtgacctt ggaggacatt
     1561 cttgaggaaa ttgttggcga tatcgaggat gagtatgatg aaagttatgt tctgatcgaa
     1621 cggattggag ataatgtata cgaagccgat ggggctacgc ctttgcatcg cttcaatgat
     1681 tattttggta cgcaacttga gtcagcggat gtcgatacga tcgccggtta tctgttgacg
     1741 gagttg
//
LOCUS       DAOMUJ010000407         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19366_length_1730_cov_1.594627, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000407 DAOMUJ010000000
VERSION     DAOMUJ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19366_length_1730_cov_1.594627"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>704)
                     /locus_tag="PLT73_08790"
     CDS             complement(<1..>704)
                     /locus_tag="PLT73_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM20503.1"
                     /translation="VLFDAVEIAKVKGHAGIASYVNGVLRNVQRNGLRDWKTISNAKK
                     RISVGASMPKWLVAYFIERIGIEETEKMAFSLLEDPFVSLRLQSNEISREEAIGLLKA
                     EGYDVVASPVSPVGIRIHGGKIVDSPLFKDGMLTIQDESSQLVAPVGELEKDFLVLDS
                     CAAPGGKTTHMASYLSASENGKVIALDMYEHKISLINQNAERLHVSDRIETHVLDAKE
                     AVHAFAPESFDVIYVDA"
     assembly_gap    705..714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<715..1017)
                     /locus_tag="PLT73_08795"
     CDS             complement(<715..1017)
                     /locus_tag="PLT73_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRM20504.1"
                     /translation="MDNKLKNKIKHTVRYLAVDILESVENEGAYSNLLLNKVILSEKL
                     SPKDAGLLTEIVYGVIQRKMTLDYGLSSYIKDPKKQLSWLNNLLRVSAYQMVYLTKI"
     gene            complement(1007..>1730)
                     /gene="fmt"
                     /locus_tag="PLT73_08800"
     CDS             complement(1007..>1730)
                     /gene="fmt"
                     /locus_tag="PLT73_08800"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRM20505.1"
                     /translation="LNPDLIVTAAYGQFLPVKLLNAPRFRSINVHASLLPKYRGGAPV
                     HYAIINGEIETGVSIMYMEKKMDAGAVLAQRAIPITEQDDVGTMFLKLSELGKNLLIE
                     TLPDFFQGNLVPQEQDESAATFSPNIKREEERIDWSKTAAQVAFQVRGMRPWPVAHTL
                     LDGQRIKVWAGQAVENSTTHDSGTVFATDKEALYVACGEGTVFKMTELQPAGKKRMSA
                     ADYLRGSACEIHEGTRFETDGQ"
BASE COUNT          399 a          460 c          406 g          455 t
ORIGIN      
        1 gcatcaacat aaatgacatc aaaggattcc ggtgcgaatg cgtgaactgc ttctttcgca
       61 tccagaacat gggtttcgat gcgatcggac acatgcagac gctcggcatt ttggttgatc
      121 aagcttatct tatgctcata catatccaaa gcgatgactt tgccgttttc cgaagccgag
      181 aggtaactgg ccatgtgcgt cgtttttcca cccggtgcgg cacagctgtc caaaaccaga
      241 aaatcctttt ccagttcccc gaccggtgcg accaattggc tagattcatc ctgaatcgta
      301 agcatgccgt ctttgaatag cggggagtca acgattttcc caccgtggat ccggatgccc
      361 acaggagaaa caggactcgc gaccacatca taaccttccg ctttgagcag tcctatagcc
      421 tcttcccgtg agatttcatt tgactgcaac ctgagtgaga caaacggatc ctccaataag
      481 gagaacgcca tcttttcggt ttcctcgata ccgatccttt cgatgaaata agcgacgagc
      541 catttcggca tgctggcccc tacgctgatg cgcttcttgg cgttgctgat tgttttccag
      601 tcgcgaagcc cattgcgttg cacattccgc agcacgccgt tcacataact ggcgatgccg
      661 gcatggccct taactttcgc tatttctacg gcatcgaaaa ggacnnnnnn nnnnaatctt
      721 cgtcaaatag accatttgat aggcggagac acgcagtaag ttgttgagcc aagaaagctg
      781 ttttttggga tctttgatgt atgacgaaag accataatcc aaggtcattt tccgctggat
      841 gacgccgtag acaatctcgg tcaacaggcc ggcatcctta ggcgacaact tttcggatag
      901 aatgacttta ttcagcagaa gattggaata cgcaccctca ttctccacgc tctccaatat
      961 atcgacagcc aggtaacgga ctgtgtgttt gattttattt ttcagtttat tgtccatccg
     1021 tttcaaacct tgtgccttca tgaatttcgc aggcgctgcc ccgcaggtag tctgcagctg
     1081 acatgcgttt tttcccggca ggttggagtt ccgtcatttt gaagacggtg ccttccccac
     1141 atgccacata gagagcttcc ttgtctgttg cgaatactgt tcccgagtca tgggtcgtac
     1201 tattttcgac agcttgtcca gcccatactt tgatcctttg cccatccaaa agcgtatgcg
     1261 ccactggcca cggacgcatg ccacggactt ggaaggccac ttgtgcagcc gttttggacc
     1321 aatcgatgcg ttcctcttcg cgcttgatgt tcggcgaaaa agtggctgcg gattcatcct
     1381 gctcttgcgg cacgagattg ccttggaaaa aatcaggtaa cgtttcgatc aaaagattct
     1441 tgccgagttc gctcaacttc aaaaacatcg tcccgacatc atcctgctcg gtgatgggga
     1501 tggctctctg cgccaacaca gcccctgcat ccattttctt ctccatgtac atgatggaga
     1561 ctccggtctc tatttccccg ttgatgatgg cgtaatggac cggtgcgccg ccgcgatatt
     1621 tcggcaataa agaagcatgc acattgatgg agcggaaacg cggagcgttc agcagtttga
     1681 ccgggagaaa ctgcccataa gccgccgtca caatcaggtc aggattcaag
//
LOCUS       DAOMUJ010000408         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19486_length_1722_cov_1.767846, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000408 DAOMUJ010000000
VERSION     DAOMUJ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19486_length_1722_cov_1.767846"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLT73_08805"
     CDS             complement(<1..798)
                     /locus_tag="PLT73_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM20506.1"
                     /translation="MKSELQKYFGYPAFKEGQLDPILSVLAGKHTLATLPTGTGKSLI
                     YQLSGYLLDGMVVIVSPLLSLMEDQVAQLKFMGEKRTASLNSMLQFEERQKLLSNLDQ
                     IKFLFLSPEMLQNQFVMNQLKQIPLALFVVDEAHCISQWGLDFRSDYLFLGAARQALG
                     NPLTLALTATATSAVMGDIIGSLHIDGNALNVFEGPVDRANIYYKVAAMPPEEKNEHL
                     LELLKRYPSPGIVYFSSKAQADAVAFMIRKKTSLRVEAYHADRTNEDR"
     gene            complement(806..>1396)
                     /locus_tag="PLT73_08810"
     CDS             complement(806..>1396)
                     /locus_tag="PLT73_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM20507.1"
                     /translation="GDKAAFARAYGEEWRSYLSRLPERDAGLFVLQFEGADHLRKTTG
                     QMAGLLSFEEAEVKILLHQNWLRLYGTLEKEPENFPVLASVMQMTIKENGLASASAET
                     TMGYFMHGHGMAEIISMRGLKQSTIQDHFAEIALIYPEFPTQRFLDKEKMQYLQQLVE
                     HKVRVDYREIQKVFPEITFFESRLIQIRSEVAAKYG"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1407..>1722)
                     /locus_tag="PLT73_08815"
     CDS             complement(<1407..>1722)
                     /locus_tag="PLT73_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20508.1"
                     /translation="FTVMPGMKPTALKKGLERLVSDGLISGNEGEGFLLSEAGSAKKA
                     EFFSRHAHFSLGSQLEFALVQSVLRRRMLFLIQVLSEMLHKNKHYYPVEREAKEQLWM
                     KR"
BASE COUNT          424 a          440 c          376 g          472 t
ORIGIN      
        1 tctgtcttca tttgtccggt cggcatgata ggcttctacg cgcaaacttg ttttttttcg
       61 gatcatgaaa gctaccgcat cagcttgtgc cttactggag aagtaaacaa tacctggaga
      121 tgggtatctc ttcaaaagct caaggagatg ttcgtttttc tcttctggag gcattgcagc
      181 gaccttataa taaatgttgg cacggtctac cggtccttca aatacgttca aagcgttccc
      241 atcgatatgc aaagacccga tgatgtcacc catcacggct gatgtcgctg tagcggtgag
      301 cgccaacgtc aatggattgc ccaaggcttg tcttgctgct cccagaaaaa gatagtcgga
      361 gcggaaatcc aatccccatt gggaaataca gtgcgcctca tcgaccacaa acaaagcaag
      421 cggaatctgt ttgagttggt tcattacgaa ttggttctga agcatctccg gagaaagaaa
      481 caaaaacttg atttgatcca ggttgctcag taatttttgc ctttcttcga actgcagcat
      541 gctgtttaat gaggccgtcc ttttttcacc catgaatttc agctgggcga cctggtcttc
      601 catcaaggac aacaatggcg aaacgataac gaccattcca tccaaaagat agccgctcag
      661 ctggtatatc aacgattttc ctgttcccgt cggcaatgtg gccaacgtat gtttgccggc
      721 caaaactgat agaatgggat ccaattggcc ctctttgaat gccgggtagc cgaagtattt
      781 ctgtagttct gatttcaatt gtgcttcatc catatttagc cgcaacctca cttcttattt
      841 gtatcagcct ggactcgaaa aaggtgattt cgggaaatac tttttggatt tcccggtaat
      901 caacacgtac cttgtgttcc acgagttgct gcagatactg catcttctcc ttatccaaaa
      961 atctttgtgt agggaattcc ggatagatca gggcgatttc ggcaaagtga tcttggatcg
     1021 tgctctgttt caatccccgc atcgaaatga tttccgccat gccatgcccg tgcatgaaat
     1081 atcccatggt cgtttcggcg ctggcggaag ccaagccgtt ttcctttatc gtcatctgca
     1141 tgacagaagc aaggacagga aaattttccg gttctttttc caaagtgccg tacaacctca
     1201 gccaattttg gtgcagcagt attttcactt cggcttcctc gaacgataac agtccagcca
     1261 tttgaccggt cgttttcctc aagtggtcag cgccttcaaa ttgcagcaca aagagcccgg
     1321 catccctttc aggcagccgt gacaaatagc tacgccattc ttccccataa gcgcgtgcaa
     1381 aagcggcttt atctccnnnn nnnnnnaagc gcttcatcca cagctgctcc ttcgcttccc
     1441 gctcaacagg atagtaatgt ttatttttgt gcagcatttc ggaaaggact tggatcagaa
     1501 acagcatccg acgacgcaaa acactctgaa cgagggcaaa ttccagttgg cttcctaaag
     1561 aaaaatgcgc gtgacggctg aaaaactcag ctttcttcgc gcttcctgct tcgctgagca
     1621 gaaagccctc gccctcgttt ccgctgatca gtccatccga aaccaaacgc tcgagaccct
     1681 ttttcaaggc tgtcggtttc atgcccggca tgacagtgaa at
//
LOCUS       DAOMUJ010000409         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19523_length_1719_cov_1.673077, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000409 DAOMUJ010000000
VERSION     DAOMUJ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19523_length_1719_cov_1.673077"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLT73_08820"
     CDS             complement(<1..954)
                     /locus_tag="PLT73_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRM20509.1"
                     /translation="MIHSKYTIRENNEATILNAIISQKEISRAELSVLAGLNKASVSS
                     IIKKLLDDELIHEDRIGDAARIGGRKPIMLTFNGKAALTLSLDVAPNYVEGLIAYIDG
                     KVLYESEIRGIKVSSDNVLAYITEMVEKLTQHAVETPHGITGLCVAIHGLVTQEEIIF
                     TPNYTLQSDLKAQLEEMYPFYVYLENEANLAALGEYTFGSISDSVVSLSIHTGIGAGI
                     VENGKLRTGKKGIAGEIGHSILYPDGRKCPCGNDGCLEQYASHQAIYSELEQLLALPD
                     INSSLVIERYKKGDPVVQKTLRTNAHYLSIAVNNLAVIYEPD"
     gene            1111..>1719
                     /locus_tag="PLT73_08825"
     CDS             1111..>1719
                     /locus_tag="PLT73_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRM20510.1"
                     /translation="MSYFSTVDKIKYEGVTTENPMAFRHYNASEMVMGKTMKDHLRFA
                     VAYWHTMTQDGSDPFGSPTNIRTWEGSTPMETAKNRVEAFFEILEKLGVDYFCFHDVD
                     IAPEGDSLEEFFNNIDEITDLIKAKMDQTGIKLLWNTANMFSHPRYVNGAASSNSAEV
                     FAYAAAQVKKGLDISKKLGGENYVFWGGREGYESLLNTDMKFE"
BASE COUNT          480 a          386 c          395 g          458 t
ORIGIN      
        1 atccggttcg tagatcaccg ccagattatt gacggcgatg ctcaaataat gcgcattggt
       61 ccgaagtgtt ttttggacaa caggatcgcc ttttttgtag cgttcgatca ctaaggacga
      121 gttaatgtcc ggcagtgcaa gcagctgttc cagttcacta tagatggctt ggtgggatgc
      181 gtactgttcg aggcaaccgt cattgccgca aggacacttc cggccatcag gatagaggat
      241 actgtggcca atttcaccgg ctataccttt tttcccggtc ctcagtttcc cattttcgac
      301 aattccggct ccgatgcccg tatggatgct caggctgacg acgctgtccg aaatggatcc
      361 aaaagtatat tcaccgagcg ccgccagatt cgcttcattt tccaaatata cataaaaggg
      421 atacatttct tctaattgag ccttcagatc gctctgcaaa gtatagttgg gtgtaaagat
      481 gatttcttcc tgagtgacga gtccgtggat cgcaacgcag agtcccgtga tgccgtgagg
      541 cgtttccacc gcatgttgcg tcaatttttc aaccatttcc gtgatatagg cgaggacatt
      601 gtcggaagaa actttgatac ctctgatttc ggattcataa agcacctttc catcaatgta
      661 ggcgatgagc ccttctacat aattaggggc aacatccaaa gaaagggtaa gcgcagcttt
      721 tccattaaaa gtgagcatga tgggcttccg gccgccaatt cgtgcggcat cgccaatccg
      781 gtcctcatgg atcaactcgt catccaacag tttcttaatg atcgaggata ccgaagcctt
      841 attcagaccg gcaagcacgg ataattcagc gcgggaaatt tctttctggc tgatgatggc
      901 attcaaaatg gtcgcctcat tgttttcgcg gattgtatat ttgctatgaa tcaataaaac
      961 cactccttct tccttcgtta tctatcatat atcttctgaa agcgcttgac aacaaaaaaa
     1021 taatcgttta tattatgttc atgagttagt tgagcgaact aacccaaagg gaagttaaca
     1081 ataagatacc atttttgagg aggaaaagaa atgagttatt tttcaacagt agacaaaatc
     1141 aaatatgaag gcgttaccac agaaaatcca atggctttcc gtcattataa tgcatctgaa
     1201 atggtgatgg gcaaaacgat gaaagatcac ttgcgcttcg cggtagctta ctggcacaca
     1261 atgacgcagg acggttccga tcctttcgga agccctacga acatccgcac atgggaaggg
     1321 tccactccaa tggaaaccgc aaagaaccgc gtggaagctt tctttgaaat cttggaaaaa
     1381 cttggcgttg actatttctg tttccatgat gtggatatcg caccagaagg cgattcgctg
     1441 gaagaattct tcaacaacat cgatgaaatc acggatctga tcaaagcaaa aatggatcaa
     1501 actggcatca aactgttgtg gaatacagca aacatgttct cacacccacg ttatgtgaac
     1561 ggggcagctt cctccaatag cgctgaagtt tttgcctatg cggcagcgca agtgaaaaaa
     1621 ggcttggaca tcagcaagaa attgggcggc gaaaactatg tcttctgggg cggccgtgaa
     1681 ggctacgagt ctttattgaa tacagacatg aaattcgag
//
LOCUS       DAOMUJ010000410         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19663_length_1710_cov_2.357704, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000410 DAOMUJ010000000
VERSION     DAOMUJ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19663_length_1710_cov_2.357704"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1536)
                     /gene="mgtE"
                     /locus_tag="PLT73_08830"
     CDS             complement(169..1536)
                     /gene="mgtE"
                     /locus_tag="PLT73_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM20511.1"
                     /translation="MEPQYEFELDEAFAKIRESLQGEDMEKFRSDFLDYHLYDQAQIY
                     LALAPEERIAMYNYLSVNEMADMFEIIEEDVESIEAYLLEMNNQYAADMLANMYADNA
                     VDMLKQLKDERIWLYLRLMPIEAAKEIRALLNYEDETAGAIMTPEFVSIVANQTVRSA
                     MAILKSKAPDAETIYYIYVVNVDNVLVGVISLRDLIVNDEDMMIGDIMSERIVSVNVN
                     EGQEDVAHKIRDYDFLAVPVVDENNVLLGIITVDDIIDVIDEEAVSDFSGLAGVDVEE
                     QSENPFVAASKRLPWLITLLFLGMGTSTLISQYEGLISDASILSVFVTLITGTSGNAG
                     TQSLAVAVRKIGMQDDEEKNLFRTILSELATGVISGLVTGVTIALVIGVWKQNFILGG
                     IIGISMLVAITVANLAGSLIPVLMDRLGFDPAVASGPFISTFSDLTSVLIYFNIAKHF
                     INTLM"
     gene            complement(1520..>1710)
                     /locus_tag="PLT73_08835"
     CDS             complement(1520..>1710)
                     /locus_tag="PLT73_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20512.1"
                     /translation="DLYGGKADPEILRQALHCESLAFVHPISGEPLKIEAPLPDDFIK
                     WESRQRDKEADVRVGTTI"
BASE COUNT          457 a          442 c          371 g          440 t
ORIGIN      
        1 agcggacgtt tttttgtccg tgtgtggtag tcagcttagg cacagatttc tgggagtcag
       61 aacacgtttg atagagcgtg acaattcgaa tttagggcat gagcactaac ctcgttaaaa
      121 aataggcaat ggacaaagcg tttgcttgtt cattgcctat ttttgcacct acataagcgt
      181 gttgataaaa tgttttgcga tattgaaata gatcaaaacg gatgtcaaat cgctgaatgt
      241 cgagatgaac ggtccgcttg cgacggcagg atcaaagcca agacgatcca tcaataccgg
      301 aatcaagctg ccggccagat tcgcaaccgt gatggcaacc aacatggaaa tgccgatgat
      361 cccacccaaa atgaaattct gtttccaaac accgatgacg agcgcaatcg tgacaccagt
      421 aaccaatccg gaaatcaccc cggtcgccaa ctcggataaa atggtacgga acaaattttt
      481 ctcctcatca tcctgcatgc cgatcttgcg gactgcgacg gcaagcgatt gtgtccccgc
      541 attaccggat gttccggtga tcaaggtgac aaacacggaa aggatcgatg catccgaaat
      601 caaaccttca tattggctga tcaaagtgga tgttcccata cccaagaata atagcgtaat
      661 caaccacggc aaccgcttgg atgccgccac aaaaggattt tccgattgct cctcaacgtc
      721 gaccccggcc aaaccggaga agtcgctgac ggcttcctca tcgatgacgt caatgatgtc
      781 atcgacggtg atgatcccca gcaatacatt gttttcgtcg actaccggta cagccagaaa
      841 gtcatagtcc ctgattttat gagctacatc ttcttgccct tcgtttacgt tcacggaaac
      901 gatccgctcg ctcatgatat cgccaatcat catatcttca tcgttcacga tcagatccct
      961 caaggaaatg acgccgacca gtacattatc cacattgacg acgtaaatat agtagatggt
     1021 ttccgcatca ggcgctttac tcttgaggat ggccatcgct gaacgaaccg tttggttggc
     1081 gacgatcgag acgaattccg gcgtcatgat ggccccggca gtctcatctt cataattcaa
     1141 cagggcgcga atttcttttg ccgcctctat aggcataagg cgcagataca accaaatccg
     1201 ctcgtccttc agctgtttca gcatatcgac ggcattatcg gcatacatgt tggccaacat
     1261 atcagccgca tattgattgt tcatttccaa aagatacgct tcgatcgact cgacatcttc
     1321 ttcgataatt tcgaacatgt cagccatttc atttacggag agataattgt acatcgcaat
     1381 acgttcctca ggcgccaggg ccagatagat ctgggcctga tcgtagaggt gatagtccaa
     1441 aaaatcggaa cggaattttt ccatatcctc gccttggagt gattcgcgga ttttcgcaaa
     1501 cgcttcgtcc agttcaaatt catattgtgg ttccaacacg gacatcagcc tccttgtctc
     1561 tctgtctgct ttcccatttg ataaaatcat ccggcaacgg cgcttctatc tttaagggct
     1621 ccccactgat cggatgcaca aatgccaatg actcacaatg cagggcttgc cgcaatattt
     1681 ctgggtcagc ctttcctcca taaaggtcat
//
LOCUS       DAOMUJ010000411         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19729_length_1706_cov_2.579649, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000411 DAOMUJ010000000
VERSION     DAOMUJ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19729_length_1706_cov_2.579649"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLT73_08840"
     CDS             complement(<1..132)
                     /locus_tag="PLT73_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004901157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRM20513.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAIANYQDIEVVGINDLLEPDYLAY
                     "
     gene            complement(243..737)
                     /locus_tag="PLT73_08845"
     CDS             complement(243..737)
                     /locus_tag="PLT73_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20514.1"
                     /translation="MVRAKLVLVFALGLTAWSAQSQRLQPHADSTNTPSRPYCTQSKE
                     SADGIIEPSICELQLPLIAKIEVHPKPKGFKQPSDEPDCRTFQPAGADILRFFAHAGS
                     VNEQDWMHQLDLTRCHVSGAAYLIDGRKAKWTVYPSRMGRINIEGDKSIFLYCPGCSF
                     KPFW"
     gene            complement(731..1519)
                     /locus_tag="PLT73_08850"
     CDS             complement(731..1519)
                     /locus_tag="PLT73_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRM20515.1"
                     /translation="MGHAVIPGALCGSKSLSLDGGNSGQFRHFSPGPICAEQMGTTAL
                     LDSMGRRTKNPGSELDMDADGVARNPKILQSTPAGQSSISKNIERGSMPKVSGVIVHQ
                     TNSPNTSSVFSSYKNAGANGAHFLIDKDGTIYQTASLHQRTNHVGRLKSRCEAESRCS
                     PEESRLNQRWDPKGMHARESVKPAGIRYPSNEDSIGIELVGMAHPAPGKDEPVYEEAT
                     EAQNAALAWLVNQLQLEYGFPQTEVFRHPDVSWKNVTEASTARW"
BASE COUNT          369 a          467 c          443 g          427 t
ORIGIN      
        1 gtaggccagg tagtcgggct ccagcaggtc gttgatgccg acgacctcga tgtcctggta
       61 gttggcgatg gcggcgcgga acaccatgcg cccgatgcgg ccgaagccgt tgatgccgat
      121 tttgatggtc attgcagttt ccttttaacg agttgctgat taatttgaat agtcacccat
      181 cgcggccatg gcgctactgt ctggatacga tgcgggggta catatattta cgcttgatgt
      241 cttcaccaga atggcttgaa gctgcaaccg ggacaataca aaaaaatgct cttatcaccc
      301 tcgatattta tccgacccat ccgtgacgga taaaccgtcc atttcgcttt tcggccatcg
      361 atcaaatacg ctgccccaga cacatgacag cgcgtcaaat caagctgatg catccagtct
      421 tgttcattga ccgatcctgc atgagcgaaa aagcgtaaaa tatccgcccc tgctggctga
      481 aatgtccgac agtctggctc atccgagggt tgcttgaagc cctttggttt cgggtgcact
      541 tcgatttttg cgatgagcgg caactgcaac tcacaaatcg atggctcgat tattccgtct
      601 gcggattcct tgctctgcgt gcaatatggg cgtgatggcg tatttgttga gtctgcatgc
      661 ggctgaagcc tttgtgactg ggcagaccaa gccgtcagac ccaacgcaaa aaccaggacg
      721 agttttgccc ttaccatcga gcagtgctgg cttccgtcac atttttccaa gacacatctg
      781 ggtgcctgaa aacctcggtt tgtggaaagc catattccag ttggagctga ttcacaagcc
      841 aagccaaagc ggcattctgc gcctcagtgg cctcttcata aaccggctca tccttgccgg
      901 gcgcgggatg cgccatgccc accaattcaa tgccaatcga atcctcgttg gagggatagc
      961 gaatgcccgc tggcttgacg ctctctcgtg cgtgcatgcc ttttggatcc cagcgttgat
     1021 tgagcctgct ttcttcaggg ctgcagcggc tttcagcctc gcatcgactt ttcagtcgcc
     1081 ctacgtgatt cgttctttga tggagcgaag ctgtttggta aatcgtgccg tctttatcaa
     1141 taagaaagtg cgcaccgttg gcgccagcat ttttatagct gctgaaaacg gatgacgtat
     1201 tggggctatt ggtctggtgc acgatcacac cgctgacctt cggcatcgag ccgcgttcga
     1261 tatttttgga gatactggac tggccggccg gcgtactttg aagaattttc gggttgcgtg
     1321 ccacgccatc ggcatccatg tccagctccg accctggatt tttcgttctg cgacccatag
     1381 agtccagtaa tgccgttgtg cccatttgtt ctgcacaaat cgggccgggg ctgaaatgac
     1441 ggaactgacc ggagtttccg ccatcaagcg aaagagactt tgatccgcac aaggctcccg
     1501 gtatcacagc atggcccatc gcaattcctt tacatgcttg tcaagcagct ctttcaaaaa
     1561 cgatgatatc agtgcaacct atggatgaag caccttgcgc acagtggcga ccacattctc
     1621 cggcgtgaac ccaaagaact cgaacagctt gggcgcgggc gccgattcgc cataacggtc
     1681 aatgccgacc acggcggcgc aaccgt
//
LOCUS       DAOMUJ010000412         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19776_length_1703_cov_1.815534, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000412 DAOMUJ010000000
VERSION     DAOMUJ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19776_length_1703_cov_1.815534"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLT73_08855"
     CDS             <1..190
                     /locus_tag="PLT73_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20516.1"
                     /translation="LEKEAEKLTKEVDRVESKLNNEKFVSKAPQAVIDGEKQKGTEYR
                     EKLAAVEERIAALKEQLA"
     gene            281..>1174
                     /locus_tag="PLT73_08860"
     CDS             281..>1174
                     /locus_tag="PLT73_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRM20517.1"
                     /translation="MFATYEEAMEWIHTRKGMGPKPGIVRMKWLMEKLDHPERKFRSI
                     HIAGTNGKGSNVAYLRSLFMAAGYTVGTFTSPHIVSFNERISVNGANIPDEDVLEQAN
                     ILYALYEEIGRTDMGPLTEFEVVTAMMFSYFGTHPCDVVLLEVGLGGLYDSTNVAEPD
                     FSLITTIGKDHSNILGDTIAEIAYQKAGIIKQGTPVVIGRLPEEAFAVMRDTAEKKAA
                     PLHAFGPDFSVSNWHEGSDYHEVFDFASQLGAFEKLEITLMGGHQVDNAATALQTFLL
                     FCKKYALSYSETTIKSGLLGTA"
     assembly_gap    1175..1184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1185..1582
                     /locus_tag="PLT73_08865"
     CDS             <1185..1582
                     /locus_tag="PLT73_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRM20518.1"
                     /translation="KPFIMLDGAHNIPAMAVLTEAIKKKFSDKNITILLAALADKDLE
                     EMGQLIKSIPASKLHVTTFDFPRAASVKELCERVGVPEAGAYEDWRFAIDDLKAKQAE
                     AGMLLITGSLYFLSEVRHYLLKDKEFGDA"
BASE COUNT          489 a          345 c          453 g          406 t
ORIGIN      
        1 tctcgaaaag gaagcggaga aattgaccaa agaagtcgac cgtgtggaaa gcaaattgaa
       61 taacgaaaaa tttgtcagca aagcccctca ggctgtaatc gacggggaaa aacaaaaagg
      121 gaccgaatac cgcgaaaaac tggcggccgt tgaagaacgc atcgctgccc taaaagaaca
      181 gctagcttaa taagagaatc gaattgaaca ggaagaaccc tattagcaag ttcctttgta
      241 gatagggttc ttcttaaaca gaaatggagt gaagaggtag atgtttgcaa cttatgaaga
      301 agcgatggaa tggatccata cccgcaaagg catggggcct aagcccggaa tagtccggat
      361 gaaatggttg atggaaaaac tggatcaccc ggaacgtaaa ttcagatcca tccatattgc
      421 cggaacaaac ggaaaaggat cgaatgttgc ttacttaaga agcctgttca tggcggccgg
      481 ctatacggtg ggcaccttca cctcgccgca tatcgtcagt ttcaatgaaa ggatcagcgt
      541 gaatggggcg aatatcccgg atgaagacgt attggagcaa gcgaacatcc tttacgcact
      601 ctatgaggaa atcggccgca cggatatggg tccactgacg gaattcgaag tagtgacagc
      661 catgatgttt tcctattttg ggacacatcc ttgtgatgtc gtattgttgg aagtcggtct
      721 gggcggtttg tacgacagca cgaatgttgc cgagccggat ttttcgctca tcacgacgat
      781 aggcaaagac cacagcaata tcctcgggga taccattgcg gagatcgcct accagaaagc
      841 cggaataatc aagcaaggga cacctgtcgt catcggaaga ttgcctgaag aggcttttgc
      901 ggtgatgcgt gacactgcgg aaaaaaaagc tgcgcccttg catgcattcg gcccggattt
      961 cagtgtttcc aattggcatg agggttccga ttatcatgag gtctttgact ttgcctctca
     1021 gttgggcgct tttgaaaaac tggaaataac cttgatgggt ggccatcaag tcgataatgc
     1081 agccactgcg ctacaaactt tcttactgtt ctgcaaaaaa tacgctttga gttactcgga
     1141 gacgacgatc aaatccggtt tgctggggac agctnnnnnn nnnnaaaaac cgttcatcat
     1201 gctggatggt gcccacaata tcccggctat ggcagtgctg actgaagcga tcaaaaagaa
     1261 gttttcggat aaaaatatca ccatcctctt ggcggctttg gcggataagg atctggagga
     1321 aatgggccag ttgatcaaat caattcccgc gagtaagctg cacgtgacca cttttgattt
     1381 cccgagggct gcatcagtca aagaactgtg cgagcgggta ggtgtaccgg aagctggagc
     1441 ctacgaagat tggcgatttg cgattgatga tctgaaggcg aagcaagctg aagcaggtat
     1501 gctattgatt accggttcgc tgtattttct ttccgaagta agacattatt tattaaagga
     1561 taaggagttt ggagatgcgt aaaaaagcag tcatttttga tatggatgga ttgatgttcg
     1621 atacggaaac gttgggttat caggcacaag gcgaactggc tgaagagatg gctttgccga
     1681 tagcgttcga cttcgattat tat
//
LOCUS       DAOMUJ010000413         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19824_length_1700_cov_1.752584, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000413 DAOMUJ010000000
VERSION     DAOMUJ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19824_length_1700_cov_1.752584"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLT73_08870"
     CDS             complement(<1..1437)
                     /locus_tag="PLT73_08870"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRM20519.1"
                     /translation="MKNTIMNLKNKPLTQMLALTGTLALLTACSTEASTTTASSDVST
                     TEATVESTLMSDSSYSESNSTAIIFSDQTITVDGSGAAADGSILTITQPGTYILSGTL
                     SEGQVRVETVDEADVQIVLDGATITNSTGAAIYVKSANSATITLAEGTTNTLSDGETY
                     TFEDSEDEPDATLFSKSDLILNGTGTLVIDANYAHAIKGKDDVTIAEGTYDITSVGDA
                     IKGRDSLFVSSGTFTIDAGGDGLQSTNEEEEGKGILTIESGTFTITAAADGIQAATNL
                     QILGGDFTITTGGGSANSSTTSEAWGTWGAPTEEAATTTTEETTSAKGLKATGSLAIS
                     GGTFVLDTSDDALHTNDTIQITGGDFTIASGDDGIHADNTVTIDDGTIDINQSYEGIE
                     ATEITLNGGDIALTTSDDGINAAGGNDASAVSGRPGESTFTSTAEGAGLLTINGGTLV
                     VNASGDGLDSNGSIEMNDGTVVVNGPTDS"
     gene            complement(1515..>1700)
                     /locus_tag="PLT73_08875"
     CDS             complement(1515..>1700)
                     /locus_tag="PLT73_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20520.1"
                     /translation="EIIKNRFGKVIVKSKSITDGQPELIYEVKVKNNETSFMNELSAI
                     EGIQSATLVSYNGNQAG"
BASE COUNT          319 a          409 c          457 g          515 t
ORIGIN      
        1 gctgtcggtt gggccgttga cgaccacggt accatcgttc atttcgatgc tgccattgct
       61 gtcgagtccg tctccgctag cgtttaccac tagcgttcca ccgttgatgg tcaagagtcc
      121 agcaccttct gcagtagacg tgaaggtgct ttcgccaggg cgtccgctga cggcagaagc
      181 atcgtttcca ccggcggcat tgatgccatc gtctgaggta gtcaaagcga tgtctccgcc
      241 gttcagcgtg atttcggtag cctcgatgcc ttcataactt tgattgatgt caatggttcc
      301 gtcatcgatt gtcacggtgt tatcggcatg gataccatca tccccggaag caatcgtgaa
      361 atcgccgcct gtgatttgga tcgtatcatt ggtatggagt gcatcatcgg aggtgtccaa
      421 tacgaatgta ccgccggaaa tcgctaatga tcctgtcgct ttcaaacctt tggcactggt
      481 tgtttcctcg gtggtagtgg tggccgcttc ttcagtaggt gctccccatg ttccccatgc
      541 ttcgcttgtt gtgctgctgt tcgcgctgcc gccgccggtt gtgatcgtaa agtcgccacc
      601 aagtatctgc agattggttg cggcttggat gccgtccgcg gctgcagtga tcgtaaaggt
      661 gcctgattcg attgtcaaaa ttcctttgcc ttcttcttcc tcgttagtcg actgcagtcc
      721 gtcgccgcca gcatcgatcg tgaaagtacc ggagctgacg aagagcgaat cgcgtccttt
      781 aatggcgtcg ccgacagaag tgatgtcata ggttccttct gcgatggtca catcatcctt
      841 ccctttgatg gcatgagcat agttggcatc gatgaccaac gttcccgttc cgttcaggat
      901 gaggtcggat ttgctgaaca gggtagcgtc cggctcgtct tcactgtctt cgaaggtata
      961 ggtttcaccg tcgcttaaag tattggtcgt tccttcagcc aaagtgatgg tcgcggagtt
     1021 ggcgcttttc acgtagattg cggctcctgt ggaattcgtg atggtagccc cgtcgagaac
     1081 gatctggaca tcggcttcat cgactgtttc gacacggacc tggccttcgc tcaaggttcc
     1141 gctgaggata tacgtgcccg gttgggtaat ggtcagaatg gaaccgtcag cagcagctcc
     1201 ggatccgtcg actgtgatcg tttgatcgga aaaaatgatg gctgtgctgt tgctttctga
     1261 ataactggag tcagacataa gagtagattc aactgtcgcc tccgtggtcg agacgtcgct
     1321 ggatgctgtt gtggtgctcg cttcagtaga gcaagcagtc aataaggcaa gggtgcctgt
     1381 aagtgcgagc atttgagtta gcggtttgtt tttcaagttc attattgtat ttttcatggg
     1441 tttgttctcc tgtcggatat gtttttttta gtaaataata ggtgttaatg tctagctacg
     1501 cgggttagct tttcctatcc tgcttgattg ccgttgtagc tgaccaaagt ggcgctctga
     1561 atgccttcta tggcgctcaa ttcattcatg aagcttgtct cgttgttctt tacttttact
     1621 tcatagatca attccggttg accgtccgtg atgctcttgg attttacgat gactttgccg
     1681 aaacggtttt tgatgatttc
//
LOCUS       DAOMUJ010000414         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19837_length_1699_cov_1.585766, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000414 DAOMUJ010000000
VERSION     DAOMUJ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19837_length_1699_cov_1.585766"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(355..1443)
                     /gene="chvE"
                     /locus_tag="PLT73_08880"
     CDS             complement(355..1443)
                     /gene="chvE"
                     /locus_tag="PLT73_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM20521.1"
                     /translation="MKFSLKKGILATATLLSAVVLGACGSGNASTGESGFVGIAMPTK
                     SMERWISDGNNMVTELEKLGYKTDLQYGEDKVENQIAQIENMITKGVDLLVIAPIDGS
                     ALTNVLEKAANEDIEVIAYDRLLMNSEHVDYYATFDNFGVGVLQASYIEDALDLKEGE
                     GPYNIELFSGSPDDNNALINYAGVMSVFQQYLDSGQLVVPSEQTKFNQIATLRWDGST
                     AQGRMDNLLSANYADKQLDAVLSPNDTISLGIISSLKGVGYGSADKPMPVITGQDADQ
                     AGVKSIIAGEQTQTVFKDTRVLAKNTIEMIEAIFKGEEVPVNDTETYDNSVKVVPTYL
                     ANPISVDKENYKAELIDTEYYTEEDLAQ"
BASE COUNT          411 a          456 c          340 g          492 t
ORIGIN      
        1 gcattgtcct ccgataagta tagggagcct ggtatattca ttgcagcagt cggacttcct
       61 tcgccgactg ctaaaagcgg gctgtcatac tctgatcccc tggataatgt tgtataacat
      121 catcatacat cagccattcg ctgctgtaca atcatctcca atatatcgct ggccggagtt
      181 gcgcgtctgg atcgacgcca acctccgaca agggttccca caacggagtt cagcggtaac
      241 ttgcgggctc ttctcctgcg agtcagtcct catcggagca cagaccccaa aggtaaggcg
      301 tcctccggcg aaaggccgcc tcgaaatgag acagcctgtg ttcgttccta tcgtttactg
      361 cgctaaatct tcttcagtgt aatattccgt atcgatcagt tctgccttat agttctcttt
      421 atcgacagag atcgggtttg ccaaataggt cggcaccact ttaacgctgt tgtcataggt
      481 ttcagtgtca tttaccggta cttcttcacc cttgaaaatg gcttcgatca tctcgattgt
      541 atttttagcc agaacacgcg tatctttgaa taccgtctgt gtttgttcgc ctgcaatgat
      601 cgatttcaca ccagcctggt cagcatcctg ccctgtgata accggcatcg gtttatccgc
      661 agacccgtag cccaccccct tcagagaaga gatgatccct aaactgattg tatcattagg
      721 agacaagacg gcatccaatt gcttgtccgc gtagtttgca ctcaacagat tatccatccg
      781 tccctgagca gtcgatccat cccagcgcag tgtggcgatt tggttgaatt ttgtttgctc
      841 ggaaggaacg accaattgtc cactgtcaag gtactgttgg aacacggaca tcacgcctgc
      901 ataattgatt agtgcattat tatcatctgg agatccgctg aataattcga tattataagg
      961 gccttcgcct tccttcaaat ccaatgcatc ttcgatataa gaagcctgta gaaccccaac
     1021 gccgaagtta tcgaatgtgg cataataatc cacgtgctcg gaattcatca acagacggtc
     1081 gtaagcgatg acttcaatat cctcgttcgc agctttttcc aaaacgtttg ttaaagcaga
     1141 accatcgatg ggggctatca ccaaaagatc tacgcctttc gtgatcatgt tctcgatttg
     1201 cgcaatctga ttttcgactt tgtcttcccc atactgcaag tccgttttat aacccagctt
     1261 ctccaattcc gtcaccatgt tgttgccgtc cgaaatccat cgttccatcg atttcgttgg
     1321 catcgcaatc ccgacaaaac cactttctcc ggtagaagca ttgccacttc cgcaagcgcc
     1381 caagaccact gcagataaca aagttgctgt tgcaaggata ccttttttca aactgaattt
     1441 cattccgtat tcctccactg ctgattaatt ttacatcttt accacacccg tcagggataa
     1501 cgcttacatt agatggatta tccgttccta ggcatcactt cgcgaatcgt ggctggatgg
     1561 tatgcaacca tccggccacg attcgttccc atgattcaaa cccaaaatct taacgtttca
     1621 cactcaagtg tctgcacaaa ttgaatgtta ttctttcgcc aatgcatgac acaattcttt
     1681 tgttgctccg tatacttgc
//
LOCUS       DAOMUJ010000415         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19843_length_1698_cov_3.130250, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000415 DAOMUJ010000000
VERSION     DAOMUJ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19843_length_1698_cov_3.130250"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1327)
                     /locus_tag="PLT73_08885"
     CDS             complement(128..1327)
                     /locus_tag="PLT73_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM20522.1"
                     /translation="MVLRKSYLDKIIPFIDQDLIKVLVGIRRCGKTVLLGQIKDVLLQ
                     RNIPAQNIIQANFESMRFRNTRTAETLYDYIAEKAEGCTGKIYILLDEIQEVERWQIA
                     INSLRVDFDCDIYLTGSNSKLLSGELATYLSGRYIQIQVFPFSLAEAKQQCIENGTYT
                     SDEKLFADYLKYGGFPQRFFLPDDHSITTYLDDLYEAIIVRDIMLRHNIREQTALRNV
                     LAFLLDNIGNPFSARNISGRMVSEGIKTTTATVLNYVDYFKEAFILLNASRYDIKGKA
                     LLSSTEKYYAVDLGLRNVIKKSEKLDSNKLYENIVYLEMRSRGYEVQVGKLDDTEIDF
                     ICYRGDEKLYIQVAYLITSADEEREFGNLERLHDNYPKYVISGDLMNLSRNGIIHRNI
                     IDFLLNP"
     gene            complement(1449..>1698)
                     /locus_tag="PLT73_08890"
     CDS             complement(1449..>1698)
                     /locus_tag="PLT73_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20523.1"
                     /translation="DKRQELSDQIEQMEQEKTAIEQQVKLQMQDAAYGTAPGYKVSWV
                     SSESKRVDSQRLKKEQPDIFNRYSKNVSSRRFTIIHAA"
BASE COUNT          447 a          377 c          393 g          481 t
ORIGIN      
        1 cttccagatg atctttgaag tcgatagcaa ataattttac gaatggattt tccataagaa
       61 tgcctccaat tctgataaag aaaaaagggc acaacagcgt caactgttgt gccccatgat
      121 gtgaaaatta cggattgagc agaaaatcaa tgatgtttcg atgaatgatt ccgtttcggc
      181 ttaaattcat caaatcacca ctgatgacat acttaggata gttgtcgtgc agccgctcaa
      241 gattaccgaa ctcccgttct tcatcggcgg aagtgatcag gtaagcaacc tgaatataga
      301 gcttttcatc tccacggtag cagataaaat caatttcggt gtcgtccagc ttgccgacct
      361 gaacttcata gccacggctc cgcatttcca gatatacgat gttctcatac agcttgttgc
      421 tgtcaagttt ttcgcttttc ttgataacgt tccgcaggcc aagatcgact gcatagtact
      481 tttctgtgct ggacaggagc gcttttcctt tgatatcata gcggcttgca ttcagaagga
      541 taaaggcttc cttgaaataa tcaacgtagt tcagtacggt agcagtggtt gtcttgattc
      601 cttccgaaac catgcgtcca ctgatattac gggcagaaaa cggattgccg atattgtcca
      661 gcaggaatgc aaggacatta cgtaatgcgg tctgttcgcg aatattgtgg cgcagcatga
      721 tgtcacggac aatgatagcc tcgtaaagat cgtccagata ggtggtgatt gaatgatcgt
      781 cagggaggaa gaaacgctgc ggaaaaccgc cgtacttcaa atagtctgcg aagagctttt
      841 catccgaagt ataggttccg ttttcaatgc attgctgttt tgcttcggcc agcgaaaagg
      901 ggaaaacctg aatctggatg tatcgtccgg aaagataggt tgccagttcg ccggaaagca
      961 gcttggaatt ggagccggtc aggtaaatat cacaatcgaa atcgacacga agagaattga
     1021 ttgcaatctg ccagcgctcc acctcctgaa tctcatccag aagaatatag attttgccgg
     1081 tgcagccttc cgctttttct gcgatgtagt cgtaaagcgt ttctgcagta cgggtgttgc
     1141 ggaagcgcat ggactcaaaa ttggcctgaa taatgttctg tgcgggaatg ttgcgctgga
     1201 ggagcacgtc cttgatctga ccgagaagga ctgtttttcc acagcgccgg attccaacca
     1261 gaactttgat cagatcctga tcgataaaag gaatgatctt atccaaataa cttttgcgca
     1321 gaaccatcgt gcatcacctc atattcttat cttagcatac aattattgtt gtgtaaacag
     1381 tattgtgctt ttctattaaa caaaaatagc tgaagcgcaa attttgtgtg cctataggcg
     1441 tacaaaaatt atgctgcatg gataatggta aacctgcggc tgcttacatt cttgctgtac
     1501 cgattgaaaa tatcgggctg ttctttcttc aaacgctggg agtctacccg tttactttcg
     1561 gaggataccc aggacacctt gtagcccggt gctgtgccat aggcagcatc ctgcatttgc
     1621 agcttgacct gctgctcgat agccgttttc tcctgttcca tctgctcgat ttggtcggaa
     1681 agctcctgcc gcttatcc
//
LOCUS       DAOMUJ010000416         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_19904_length_1694_cov_2.937157, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000416 DAOMUJ010000000
VERSION     DAOMUJ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19904_length_1694_cov_2.937157"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1504)
                     /locus_tag="PLT73_08895"
     CDS             complement(143..1504)
                     /locus_tag="PLT73_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM20524.1"
                     /translation="MNRIFRALTVLLTGLFLFGLNNQKDEERTVQSIAPLVGKDENLK
                     IWKVTDIHYLSPDLFDDGEAFARMKATSAGKDLEHVPDMMEALVWEAAKEQPDLLIVS
                     GDLTFNGEYQSLVELAAFFERIEKNGTQVSVIPGNHDIHSGWARKFEGEEMAVVEQAT
                     PEDFQQIFADYGYDLAISKDPESLSYVMEPKAGFPFLMIDTNIYSETKSTKAPKTEGR
                     IKEETYAWLDEYFALQEEHLQTIQLVGHHPLLNHTGRLGGGFILENAEDATDYFAGKG
                     VQTSFAGHIHAQDISEATDREAPFYEVVTGALSIFPNAIGEITLTNEELAYQRKTLDI
                     EGWAEATGVEAPELLAYTQTSHDLFKRDGENLALQMMFEEQWHEEIYEEAVMDYVGKM
                     NVRFFSGEDYVEDERSLGEWTSHAGYQIIQQNSDNFLKKYTNQLLQDKNLDDRHIVIP
                     QRQ"
BASE COUNT          399 a          447 c          354 g          494 t
ORIGIN      
        1 aaggggaaag ccacttgacc tttcagtgta tcttattgcg ggccttcatg cgtatcgtat
       61 tgacggattt cgatccctaa acctgtttcg gcaaaaaaac cggcaataag aaagaggcta
      121 ggcaacccgc ccaacctctt ccttattgcc gctgtggaat cacaatgtgc cgatcatcca
      181 gatttttatc ctgcagcaat tggttcgtgt atttcttaag aaaattatca ctgttttgtt
      241 gaatgatttg atagccggca tggctggtcc attctcccag agaacgctca tcctcgacgt
      301 aatcctcccc ggaaaaaaag cggacattca tctttccgac ataatccatg acagcttctt
      361 catagatctc ttcatgccac tgttcttcga acatcatctg caaagccaga ttctcgccat
      421 cgcgcttgaa caagtcatgg gaagtctgcg tgtaggcaag caattcaggc gcttcaaccc
      481 ctgtagcttc cgcccaacct tcaatgtcca aggttttgcg ctgataagct aattcttcat
      541 ttgttaatgt aatttctccg atcgcgttgg ggaatatcga aagtgctccg gttacgactt
      601 catagaaagg agcctcccga tccgtcgcct cggaaatatc ttgtgcatgg atgtgccccg
      661 caaagctcgt ttggacgcct tttccggcaa aatagtcggt tgcatcttcc gcattttcca
      721 aaataaaccc tccgcctaat ctgccggtat ggttcagcag cggatgatgc ccgaccagct
      781 ggatcgtctg caggtgctct tcctgcagcg caaaatattc atccaaccag gcgtatgtct
      841 cttccttgat tcttccctcc gttttcgggg cttttgtgct cttcgtttcg ctgtatatat
      901 tggtgtcgat catcaaaaaa ggaaatcccg ctttcggctc catcacatag ctcaaggact
      961 ccgggtcttt gctgatggcg agatcatagc catagtccgc aaaaatttgt tggaagtcct
     1021 ctggtgttgc ttgctcaacg acagccattt cttcgccttc gaatttccgg gcccagccgc
     1081 tgtggatatc gtggtttccg ggtatcacac tgacctgcgt cccatttttt tcgattcttt
     1141 caaaaaatgc agcaagctcc accaggcttt ggtattcacc gttgaaggtc aaatcaccac
     1201 tgacgatcaa cagatccggc tgttctttcg ctgcttccca cacaagcgcc tccatcatat
     1261 ccgggacgtg ctccaagtct ttaccggcag aagttgcctt cattcgagca aatgcttcgc
     1321 cgtcatcgaa cagatccgga gagaggtagt ggatatccgt caccttccaa atctttaaat
     1381 tttcgtcttt cccaactaaa ggtgcgatcg actgcaccgt gcgttcctca tctttttgat
     1441 tgttcaaacc aaagagaaaa agacccgtca gcaatacagt cagtgccctg aatattctat
     1501 tcataaattt ctgtcccctt gtggatcatt tggactcatt atagttgttc tgcttgccgc
     1561 ttgtcgagtt gttccctaat aaaaaagaaa cgttccggaa atcatcggta tgattccgga
     1621 acgcttcttc atttttatga ctgggctagc tggattcgaa ccaacgaatg acagagtcaa
     1681 agtctgttgc ctta
//
LOCUS       DAOMUJ010000417         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20040_length_1686_cov_2.038013, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000417 DAOMUJ010000000
VERSION     DAOMUJ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20040_length_1686_cov_2.038013"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLT73_08900"
     CDS             <1..875
                     /locus_tag="PLT73_08900"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374377.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM20525.1"
                     /translation="NWGFQYSPYSYYNEHCIILSEEHVPMHVTEETIANLLEIVTLFP
                     HYFIGSNAGLPIVGGSILSHEHYQGGRHHFPMEAAKVLRTLALEGFPEVEAEVLFWPL
                     SVIRLRSEDRDVVAAAACTIMEEWQNYNDPQAEILAESAGMRHNAVTLVARRVGEAYE
                     LDVVLRNNRTSEAFPDGIFHPHPDVQHIKKENIGLIEVLGLAILPPRLDTELNEVADY
                     LLGKTADVASCHREWAEELKQQGPFEADSVMEMIQEAVGEKFLRVIEDAGVYKRTPEG
                     QAGFYRFLSTLENK"
     gene            913..>1686
                     /locus_tag="PLT73_08905"
     CDS             913..>1686
                     /locus_tag="PLT73_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20526.1"
                     /translation="MATMKDIAERAGVSTATVSRVLNHDETLSVGKETKDRIFAAAKE
                     LNYTKTKKKKSREKILLLQWYTQEEELDDLYYQGIRLGAEERAEAEGYDVVRVFHDVT
                     FDIEKDVIGIVAIGKFGERQVQRLMDFGKPLCFIDSDQFKWKQDCVIVDFASAMEQVI
                     AEMDRGEHTKIGFLEGKELTNDKEMLVRDLRSELFLAELRKRGWYRDKYHRVGSFSTS
                     SGYEMMNQLINENRQQLPTFLFAENDAIAIGALRALQEAD"
BASE COUNT          455 a          395 c          469 g          367 t
ORIGIN      
        1 agaactgggg atttcagtat tcgccttatt cctactacaa cgagcattgc atcatccttt
       61 ccgaagagca cgtgcccatg catgtcacgg aagagacgat tgcgaacctg ttggagatcg
      121 tgacgctctt cccacattac ttcatcggtt ccaatgcggg attaccgata gtcggaggtt
      181 ccatcctcag tcatgaacat tatcaaggcg ggcgtcacca ttttcccatg gaagctgcca
      241 aagttctccg gaccttggct ctggaaggtt ttccggaagt cgaagccgaa gtactttttt
      301 ggccgctttc ggtcattcgt ctccgttcag aggataggga tgtggtcgct gccgctgcgt
      361 gcaccattat ggaagaatgg caaaactaca acgatccgca agccgaaatc ttagcggaat
      421 cggctggcat gcgccataat gcggtaacgc tcgttgcgcg ccgtgtcgga gaagcttacg
      481 agttggatgt ggtgttgcgg aataaccgga caagcgaagc gttccctgat ggcatcttcc
      541 atccgcatcc ggatgtccag cacatcaaaa aagaaaacat cggcttgatc gaagtgctcg
      601 gcctggcaat tttgccgccg cgattggata cagagctcaa cgaagttgcc gactatctgc
      661 tggggaaaac cgctgatgtc gcctcctgcc atcgggagtg ggccgaagaa ctaaagcagc
      721 aagggccttt cgaagcagat tccgtcatgg aaatgatcca ggaggcagtc ggtgaaaaat
      781 tcctgcgcgt aatcgaagat gcaggcgtgt acaagcgaac accggaaggg caagcaggct
      841 tctatcgctt tttgagcaca cttgaaaata aataatgaaa atacacagaa gaacgacgga
      901 ggtggagtaa gtatggcgac catgaaggac atcgccgaaa gggcaggagt ctcgactgcg
      961 acggtttcgc gcgtgctgaa ccacgatgag acgctttctg tcggcaagga aacgaaagat
     1021 cggattttcg cagccgcaaa agagctgaat tatactaaaa cgaaaaaaaa gaaaagcagg
     1081 gaaaagatcc tgctgctgca gtggtacacc caagaggaag aattggatga cctctattat
     1141 caaggcatcc gtttaggggc cgaagaacgt gccgaagccg aagggtacga tgtcgtacgg
     1201 gttttccatg atgtgacctt cgatatcgaa aaggacgtca tcggcatcgt ggcaatcggt
     1261 aaattcggag agcgacaggt gcagaggctg atggatttcg gcaagcccct gtgcttcatc
     1321 gactcggacc aattcaaatg gaaacaggat tgcgtcattg ttgattttgc atcagcgatg
     1381 gaacaggtca ttgccgaaat ggacagaggc gaacacacca aaatcggatt tttggaaggc
     1441 aaagagctga cgaacgacaa agaaatgctg gtccgcgatc tgcgctcgga gctgtttctt
     1501 gccgaactgc gcaagcgcgg ctggtaccgg gacaagtatc accgcgtcgg gagtttcagc
     1561 acttcctcgg gatacgaaat gatgaatcag ttaatcaacg aaaaccggca acagcttccg
     1621 actttcttgt ttgccgaaaa cgatgcaatt gcgataggtg ccttgcgtgc tttgcaggaa
     1681 gcggat
//
LOCUS       DAOMUJ010000418         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20045_length_1686_cov_1.464746, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000418 DAOMUJ010000000
VERSION     DAOMUJ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20045_length_1686_cov_1.464746"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLT73_08910"
     CDS             complement(<1..692)
                     /locus_tag="PLT73_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM20527.1"
                     /translation="MPKNLIAGGSTGLAIILNHFFGLNISLVVLIFNGLMFFIGAALL
                     GRKFALTTILSTIIFPTFLTFFRTVPALQNMTDDILLSAIYAGILCGLGVGLVFKVGA
                     STGGMDIPPLLLNKHFRIPVAVGMYAFDVILLLIQAFFSSMEEILYGLIMVFLTSIVI
                     NKVMVTGTNKMQLFIISKKFEEIKEALLYVQDVGLTLVNIETAFEGAQQQAVLCVIEQ
                     RKLSAINALVND"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1207..>1686
                     /locus_tag="PLT73_08915"
     CDS             1207..>1686
                     /locus_tag="PLT73_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HRM20528.1"
                     /translation="MKNYLLLFGSALILTACGTSTDVEVSEEASSAAMATSESVPESI
                     PAVSEEIVIAPVEEPAPQPDVSREFRNALGSAEDYLNYSSFSKQELYDQLLYEGFPED
                     AAQYAIDNVATDWNQNALQTAVDYLDYSSFSYPGLYDQLVYEGYAAEQAQYALDNVTT
                     "
BASE COUNT          524 a          390 c          340 g          422 t
ORIGIN      
        1 atatcgttga ccaaggcatt gatggccgac aacttacgct gctcgatgac gcacagcacc
       61 gcttgctgct gcgccccctc aaaagccgtt tcgatattca ccagggtcag accgacatcc
      121 tgcacataca gcaacgcctc tttgatttcc tcgaactttt tggaaatgat gaaaagctgc
      181 atcttattcg ttccggttac catcactttg ttgatgacga tggacgtcag gaacaccatg
      241 atgagcccat aaagaatctc ttccatgctg gaaaagaacg cctggatcag caataaaatg
      301 acgtcgaacg catacattcc cactgcaacc gggatgcgga aatgcttgtt caggagcagc
      361 ggcggaatat ccatcccgcc cgtagaagcg ccgaccttga acaccagccc cacacccaac
      421 ccgcacagga ttccagcgta gatcgccgaa agcaaaatgt catccgtcat gttctgcaaa
      481 gcgggcactg tccggaaaaa agtcaaaaac gtcgggaaaa tgatcgtgct caggatcgtc
      541 gtcaacgcga acttccttcc caacaacgcg gctccgatga aaaacatgag accattgaaa
      601 atcagcacga ccaaagaaat gttcaacccg aagaaatgat tcaaaatgat ggcaagcccc
      661 gtcgatccac cggcgatcag atttttcggc aaaataaacg ccgctatnnn nnnnnnnaat
      721 aatattcccg gcaatgactg caactatcgg ttttaatgta tgtaaaagtt tcaaattagc
      781 cccctccttt gtatgggata actttcagga aaaagatatc cacgccatta taacagatat
      841 atgctggata aaaagagtag tatcaaactt tttgaatcaa aatcggactg acagctatac
      901 aacacaagaa tgtcaaatat caaacaggtt atttctatac tcgtacaaat aaaatatttt
      961 attttgtgtg caatatttgt cgatttatga ggtataattc tattaacgtg agcagaagat
     1021 accgatggaa ttgaagaagt tatttaatat ccaaattaca gaccacaatc tcctgatcga
     1081 tactcttaaa aaacaaaaac tgaccgccct gattataggt ggttcgtaaa accgcgttat
     1141 ttttcctggt ttatgatatt caccgcttct gcggttgata taaaaaaact aactggagga
     1201 atcatcatga aaaattactt attattgttt ggtagtgcat taattttgac tgcctgcgga
     1261 acgagcactg atgttgaagt ttcagaagaa gcatcatcag ctgcaatggc cacctcagaa
     1321 tcagtgccag aatcaattcc ggcagtcagc gaagaaatag tgattgcccc tgtcgaagag
     1381 cccgctccgc agccggatgt ttcaagggag ttcagaaatg cactcggcag cgcagaggat
     1441 tacctcaatt attcaagttt ttctaagcaa gaattatacg atcaactctt atacgaaggg
     1501 ttccccgaag atgcagcaca atacgcgatt gataatgtcg caaccgattg gaatcagaac
     1561 gcactccaaa cagccgtcga ctatttggat tactcttctt tctcttatcc aggcttgtat
     1621 gatcaattgg tttacgaggg ttacgctgct gaacaagcac agtatgcact tgataatgtg
     1681 acaacg
//
LOCUS       DAOMUJ010000419         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20059_length_1685_cov_1.924540, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000419 DAOMUJ010000000
VERSION     DAOMUJ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20059_length_1685_cov_1.924540"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLT73_08920"
     CDS             <1..493
                     /locus_tag="PLT73_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM20529.1"
                     /translation="LTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYAIYGVEHTT
                     RLMDRGVIEIAPLAYMRGRTLEDAFIILDEAQNTTVAQMKMFLTRLGFGSKMIVNGDK
                     TQIDLPKGALSGLIDAEKKLKGIPDIAFVTFDTYDVVRHPVVANIIKAYDVKEETKHN
                     IEK"
     gene            502..984
                     /gene="ybeY"
                     /locus_tag="PLT73_08925"
     CDS             502..984
                     /gene="ybeY"
                     /locus_tag="PLT73_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRM20530.1"
                     /translation="MEIDIFDETESVQKEHTELVHSLISFAGEYLKLAPDTELSITFV
                     DNNRIQEINREYRNKDQATDVISFALNDDMDDDFPINMESISGLFPTNIGDIIISTEK
                     TLEQAESYGHSFERELGFLALHGFLHLNGYDHMTEEDEKEMFGLQKEILDAYGLKRDE
                     "
     gene            956..1381
                     /locus_tag="PLT73_08930"
     CDS             956..1381
                     /locus_tag="PLT73_08930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRM20531.1"
                     /translation="MLMDLKETSKEKMGKVGKNPNFFASVRHALDGIGIILKEEKNMR
                     SHAMLGLIPLLLAWIFESSTTEWIVIIFCIFLVIIMEFLNTIFENVVDLVTDYEYHPL
                     AKKAKDIAAGAVLVTVLFTIVIAAIIFLPEFVELYYKLI"
     gene            1392..>1685
                     /locus_tag="PLT73_08935"
     CDS             1392..>1685
                     /locus_tag="PLT73_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRM20532.1"
                     /translation="MNKKEGFKSGFVSIIGRPNVGKSTLLNRIVGQKVAIMSDVPQTT
                     RNKIQGVVTSDDSQIVFIDTPGIHKPQTRLNDFMLKSAYSTFNQFYGPYQDTYV"
BASE COUNT          493 a          339 c          405 g          448 t
ORIGIN      
        1 cctgacacgt ccagctgttg aagctggtga aaatctggga tttttacccg gagatctgaa
       61 ggaaaaagtt gacccatatc tgcgtccggt ctatgatgcg ctttatgcaa tctacggcgt
      121 tgagcacacc acacgtttga tggacagggg agtcatcgaa attgcgccgc tagcttatat
      181 gcgtggacgg acgctggaag atgccttcat catccttgat gaggctcaga atacgaccgt
      241 cgcacagatg aagatgttcc tgacgcggct cgggttcggt tcaaaaatga tcgttaacgg
      301 tgacaagacc caaatcgatt tgccgaaagg ggcgctgtca ggattgattg atgccgagaa
      361 gaaactgaag ggcatcccgg atatcgcttt tgtcaccttt gatacctatg atgtcgtgcg
      421 gcatccggtc gttgcgaaca tcatcaaagc atatgatgtc aaagaggaaa caaaacacaa
      481 catagaaaag tgagcgatta tatggaaata gatatttttg atgaaaccga atctgtacaa
      541 aaagagcaca cggagttggt ccattccctg atttcctttg cgggggagta tttgaagttg
      601 gcaccggata ccgaattgtc gatcacgttt gtggacaaca atcgtatcca ggagatcaat
      661 cgagaatacc gcaacaaaga tcaagcgacg gacgtcatca gctttgcgct gaatgatgac
      721 atggatgatg acttcccaat caatatggaa agcatatccg gattgttccc caccaacatt
      781 ggcgatatca tcatttcaac cgaaaagacc cttgaacaag cggagtccta tggtcacagt
      841 ttcgaaagag aactgggttt ccttgctttg catggtttcc tgcatttgaa tggttatgat
      901 cacatgacgg aggaagacga aaaggaaatg tttggtcttc agaaagagat attggatgct
      961 tatggactta aaagagacga gtaaggaaaa aatggggaaa gttggcaaga atccaaattt
     1021 tttcgcttca gttaggcatg ctttggatgg catcggcatt atcctcaaag aagaaaagaa
     1081 tatgcgcagc catgccatgt tgggcttgat accgcttctg ttggcatgga ttttcgaatc
     1141 gagcacaacg gaatggatcg tgatcatctt ctgcatattt ttggtcatca tcatggaatt
     1201 tttgaatacg atatttgaaa atgtggtcga tttggttacg gattacgaat atcatcctct
     1261 ggcaaagaaa gcaaaagata tcgctgccgg agcggtcttg gttacagtac tctttaccat
     1321 tgttattgca gctatcattt ttttgcccga atttgtggaa ttatattata agttaatttg
     1381 aggagttttg tatgaataaa aaagaaggtt tcaaatctgg gttcgtctcc atcatcggga
     1441 gaccgaacgt aggaaaatcc accctactga accgtattgt cggtcaaaag gtcgctatca
     1501 tgagcgatgt cccgcaaact acacggaaca aaatccaagg agtagtcact tcggatgatt
     1561 cccaaattgt gttcatcgac actcccggaa tccacaaacc gcagacgcgt ttgaacgatt
     1621 ttatgctgaa gagcgcatac agtacgttca atcagtttta cggcccatat caggatacat
     1681 acgtt
//
LOCUS       DAOMUJ010000420         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20183_length_1676_cov_1.960518, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000420 DAOMUJ010000000
VERSION     DAOMUJ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20183_length_1676_cov_1.960518"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLT73_08940"
     CDS             <1..616
                     /locus_tag="PLT73_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM20533.1"
                     /translation="IRTYGKGIVDPKVVEKWKGQLPDIAVQSSQMERRSVDAERETES
                     LKKAEFMMDKIGQEFEGVISSVTKFGIFVQLPNTVEGLVHISKMAEDYFNYIESHMLL
                     MGERTGVMYRIGQKVKIKVENSDPVTRAIDFSLVIDKDKTQSQSDQDLVKQIRSKIPK
                     GKKRKPGQSDRDSGPRKKTAKGKEPFYSRTGKKTKGKKDRGKKK"
     gene            642..1100
                     /gene="smpB"
                     /locus_tag="PLT73_08945"
     CDS             642..1100
                     /gene="smpB"
                     /locus_tag="PLT73_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRM20534.1"
                     /translation="MPKGEGKVLAQNRKASHDYIILDTIEAGMVLTGTEIKSVRKAKI
                     NLKDGYASIRNGEVFLQNVHISPFEQGNIFNHDPLRTRKLLLHKKQIKYLIEETKTTG
                     VTLVPLKVYLKNGVAKVLIGLAKGKKSYDKRDALKQKDMKREIDRAMKER"
     gene            1533..>1676
                     /locus_tag="PLT73_08950"
     CDS             1533..>1676
                     /locus_tag="PLT73_08950"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HRM20535.1"
                     /translation="MITKDKYLRLLAKEYPTAAKTVTEIINLEAIMNLPKATEHFISD
                     LHGE"
BASE COUNT          551 a          348 c          424 g          353 t
ORIGIN      
        1 gatccgcacg tatggaaaag gtatcgtcga tccgaaggtg gttgagaaat ggaaggggca
       61 attgccggat atcgctgttc agagttcgca gatggaacgc cgctccgtag atgcggaacg
      121 cgaaaccgag tcgcttaaga aggcagaatt catgatggac aaaattggac aagagttcga
      181 aggcgtcatc agttccgtta caaaatttgg tatttttgtg cagttgccta acaccgtgga
      241 aggcctcgtg catatctcga aaatggcgga agattacttt aactacatcg aaagtcatat
      301 gcttttgatg ggtgaacgca ccggggtcat gtaccgcatt ggccaaaaag tcaaaatcaa
      361 agtagaaaat tctgatcctg tcacgcgcgc catcgatttc tcattggtca tcgataagga
      421 taagactcag agccagagcg atcaggatct ggtcaaacag atccgctcga agattccgaa
      481 gggcaaaaaa cggaaaccgg gccaatccga tcgcgacagc gggccgcgca aaaagaccgc
      541 aaaaggaaaa gagccattct attcccgtac aggcaaaaaa accaaaggga aaaaggacag
      601 aggcaaaaag aagtaagcta acgaaatgaa ggtgtcgaag tatgccaaaa ggcgaaggaa
      661 aagttttagc gcagaacaga aaagccagtc atgattacat catcctggat acgatagaag
      721 ctgggatggt gctgacagga acggaaatca aatccgtccg caaagcgaaa ataaacttga
      781 aggatggcta tgcaagtatc cggaacgggg aagtatttct ccagaatgtg catatcagcc
      841 cattcgaaca gggcaatatc tttaaccacg acccgctgcg taccaggaaa ttactgctgc
      901 ataaaaaaca aatcaaatat ttgattgaag agaccaagac gacaggcgtc acgttggtgc
      961 cgttgaaagt ctacctcaaa aatggagttg cgaaagtgct catcgggctg gcaaaaggga
     1021 aaaagagcta cgataagcgc gatgcattga aacaaaaaga tatgaaacgt gaaatcgaca
     1081 gagcaatgaa ggaaagataa gcataaatgt gaataacgaa cgcgcccgca ttcctgatgg
     1141 agtgcaggcg cgttttttcg gtatgagtgg taaattactg tcagctccgg cgaagccgtc
     1201 tttcgtcgga ggagcattta gaatccgcac ccagctccga cgaagcaggc ttcgccggag
     1261 gagcggatca tgttgtcttc gccaactccg atgaggcaac cctcaccgga gcaggcgcac
     1321 tccggtttgg actacagcaa ccatcggaca ggtacactgt cgaaattgtg accaattgtg
     1381 taatttttgt atcttgtcta tcattttttt taaattcaag ctatgataga agtaatcatc
     1441 ttgtcacgaa aaataaagat aatcctgtaa gcaacgtttg ccttgcgggg ttccattctg
     1501 agtgaaatga aaaaagtagg ggtgtaattg taatgataac gaaagacaag tatcttcgat
     1561 tgctggcaaa agaatatccg acagcagcaa aaacagtaac cgaaatcatc aacttggaag
     1621 ccatcatgaa cttgccgaaa gcaaccgagc atttcatcag cgacttgcac ggggag
//
LOCUS       DAOMUJ010000421         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20198_length_1675_cov_1.983951, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000421 DAOMUJ010000000
VERSION     DAOMUJ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20198_length_1675_cov_1.983951"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLT73_08955"
     CDS             <1..1427
                     /locus_tag="PLT73_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM20536.1"
                     /translation="FYPHLHSPIYTISPLFTIPSVGCKKYELNYRLMGRCAAEQLIKR
                     KENKRSEFQPMILHNDGMRNWLSKIPGSTCKRLTILTLDSPTARIMENMARIYTDATG
                     IEIKVAICSYDGIHEILSDLGNTDAYDVIRLDHTWLSWFGEHIFAPLSELTSSSAEQL
                     FEPFIPGLVPQYTSVNGVAYAFPETPSAQLLFYRKDLFENTVLQRLYKEQYKEELAPP
                     QDFEHFNRIARFFTRRFNEHSPTTYGTTLTLGNSGVAATEYLTRYFSHSHDLFDANGN
                     LLLNTDIAIQSMKELIEAKDYSPKRYNSWWRESAREFAAGDTAMSIVFSNYASEMMDS
                     DSVIINKIGYTYLPGQNSLLGGGCIGVSKNSQNKTEAFDFIKWICSEEIATAMTLLGS
                     VSPCEKTYSNYEVLDTYPWLGLSHKCIAQSKINRIPPQKATCFDERRFLSILGMAVNT
                     VYNDTATPQDALTYAIQSYNRTMK"
     gene            complement(1485..>1675)
                     /locus_tag="PLT73_08960"
     CDS             complement(1485..>1675)
                     /locus_tag="PLT73_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20537.1"
                     /translation="VPYADPIYAIVTLDENGASVKGVEGHFVPPSPEKTGITVPLTPS
                     VASWSFAWDEFETLQGDV"
BASE COUNT          481 a          384 c          359 g          451 t
ORIGIN      
        1 cattctatcc ccatcttcat tcgccaattt ataccatttc gccgcttttt acaattccat
       61 cggttggatg taaaaaatat gagttgaact accgtctgat gggacgctgt gctgcagagc
      121 agcttattaa gcggaaagaa aacaaacgat ccgaatttca accaatgatt ttgcataacg
      181 atgggatgcg gaattggtta tctaaaattc cgggctcgac ttgcaaacgg ctgacgatcc
      241 tgacactgga cagcccgaca gctcgaatta tggagaacat ggcgcgtatt tatacggatg
      301 caacaggaat cgaaatcaaa gtcgccatct gttcttatga tggaattcac gagattctga
      361 gcgatcttgg aaacacagat gcctatgatg tcatccgtct ggatcacaca tggctttcat
      421 ggtttggtga acatattttt gcgcccttga gtgagttgac atcttccagc gccgagcagt
      481 tgttcgagcc ttttatcccc ggccttgttc cacaatatac aagtgtgaat ggcgttgcct
      541 atgcctttcc tgaaacaccg agcgcacagt tactgttcta ccgtaaagat ttgtttgaga
      601 acaccgtcct gcagcgtctt tataaggaac agtataagga agagcttgca ccaccgcaag
      661 atttcgagca ctttaaccgt atcgcaaggt tcttcactag acgtttcaat gaacattccc
      721 ccacaaccta tggcacaaca ttgacacttg gaaacagtgg cgttgctgca accgaatatc
      781 taacacggta tttcagtcac tcgcacgatt tgttcgatgc aaatggaaat ctgctcctca
      841 acacagacat cgctattcaa tccatgaagg agcttatcga agccaaggat tattctccca
      901 aacgatataa tagttggtgg cgagaatcgg cacgtgagtt tgccgcaggc gatacagcaa
      961 tgtctatcgt tttcagcaat tatgcctcag agatgatgga cagtgattca gttattatca
     1021 ataaaatcgg ttacacatat cttccggggc agaattcttt actgggtggc ggctgtattg
     1081 gagtatccaa aaacagccaa aacaaaacgg aagcatttga ttttataaaa tggatttgca
     1141 gcgaggagat tgcgactgcc atgacccttc taggcagtgt ttctccttgc gaaaaaacgt
     1201 acagcaacta tgaggtcctt gatacctatc cttggcttgg actctctcac aagtgtattg
     1261 cacagtcaaa gatcaatcgt attcccccgc agaaagcaac ctgctttgat gagcgcagat
     1321 ttttaagcat cttaggcatg gcggtcaata cggtatacaa tgacacagca actccacaag
     1381 atgccttaac ctatgccatt caaagctaca atcgcactat gaaataaaat acagcctccg
     1441 agtttaggca tagtgctgat atctcggagg ctgtattttt gtgattaaac atcaccttga
     1501 agtgtctcaa attcgtccca tgcaaaagac catgatgcaa cagaaggagt caacggcaca
     1561 gtgattccag ttttttcggg agagggtgga acaaaatgac cttctactcc ttttaccgaa
     1621 gcaccgtttt catccaacgt gacgattgca tagattggat ctgcataggg aacaa
//
LOCUS       DAOMUJ010000422         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20268_length_1671_cov_2.020421, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000422 DAOMUJ010000000
VERSION     DAOMUJ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20268_length_1671_cov_2.020421"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..605)
                     /locus_tag="PLT73_08965"
     CDS             complement(87..605)
                     /locus_tag="PLT73_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRM20538.1"
                     /translation="MGRGNLDLNVVQEILNKTKEEVENMHPVNILLAGKTGVGKSTLI
                     NNVFRERMAETGIGKPITKHLRRIAKEGMPIVLYDTRGLELGHPIQTEVKREIIEVIN
                     QSQKEGSDKAIHLVYYCINAHSSRIEVAGVDCPDAIDWSACERVEEIHRKPELTDSRD
                     IECDGGTVCHHG"
     gene            complement(655..1260)
                     /locus_tag="PLT73_08970"
     CDS             complement(655..1260)
                     /locus_tag="PLT73_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM20539.1"
                     /translation="MNPDKQSKDKRVLATKKNLLHALITLLEEKSIEEVTVRELTKRA
                     KVNRGTFYLHYEDKHDLLEKNIDALIQELQDRGKAITKTALSDATETEFRQKTVEAFT
                     DIFSYIKENERFFSVLFNGRSSYSFPLKFNTYLRGRLEQQVQTKKNVIPYEHWITAVA
                     FAYQGMIYSWVTNGMKDAPERMGEYGYYFISQSVVEITRGT"
     gene            complement(1472..>1671)
                     /locus_tag="PLT73_08975"
     CDS             complement(1472..>1671)
                     /locus_tag="PLT73_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRM20540.1"
                     /translation="LPVEFNASGRALRILSNGGILNAQEVPKARKVLVAAAMTYVAAA
                     FMSFLQLLRLLLLFGGGNNDD"
BASE COUNT          401 a          428 c          345 g          497 t
ORIGIN      
        1 tgttaaaagc ctctttcgtc tcctccggca gcaccgcaaa cgtccgctca atcaattcct
       61 tcaatccgct ttgtggaatc accagctcat ccgtgatggc atacggttcc gccatcacat
      121 tcaatatccc ggctatcggt aagttcaggt tttcgatgta tttcttcaac tcgttcgcag
      181 gctgaccaat cgattgcgtc aggacaatca acaccggcaa cctcaatgcg ggaggaatgc
      241 gcattgatgc aatagtagac caaatggatt gccttatcgg agccttcctt ttggctctga
      301 ttgatgacct cgatgatttc ccgcttgact tcggtttgga tcggatggcc caattccagc
      361 ccgcgcgtat cgtacagcac gatcggcatg ccttccttcg cgatccgccg cagatgtttc
      421 gtgataggtt tcccgatgcc ggtctcagcc atccgttccc ggaagacgtt gttgatcagc
      481 gtacttttcc cgacccccgt cttgcccgca agcaggatat tgacgggatg catgttttcc
      541 acttcttctt ttgtcttgtt cagtatctcc tggaccacat tcaaatcaag gtttcctctt
      601 cccatgacgc ttcctcctgt cgctcgagcg caaacatact tttttacgga acgatcacgt
      661 tcctctggtt atctccacca cactttggga aatgaaataa tacccatact ctcccatgcg
      721 ctccggcgcg tctttcatac cattcgtcac ccaagagtag atcatccctt gataggcgaa
      781 ggcaacggct gtgatccagt gttcataagg aatgacattt tttttcgtct ggacctgttg
      841 ctccagacgt cctctcagat aggtgttgaa tttgagcgga aaggaatagc tgcttcttcc
      901 gttgaaaaga acgctgaaga agcgttcatt ttctttgatg tagctgaaga tgtccgtgaa
      961 cgcttcaact gttttttgtc tgaactccgt ttccgtagca tcggacaaag ccgttttcgt
     1021 gatggctttc cctctgtcct gaagttcctg gatcagcgca tcgatatttt tctccaataa
     1081 atcatgcttg tcttcatagt gcagataaaa agttccccga ttcacttttg cacgtttcgt
     1141 aagttccctc acagtcactt cttcgatgga tttttcttcc aacagtgtta tcaacgcatg
     1201 caaaagattt ttctttgtag ctaaaacacg tttgtcttta gattgtttat caggattcat
     1261 tattagctcc tattttcaga gtctggtctg gaattctttt ctgatatata ctttcaaaat
     1321 gctaagatta ttatagtata gcaaccccaa gataacaagt gtaacgacta gtctcaacag
     1381 gttcatggac ctaaaaaaac tgctccggcc cgttgaaata gacctaagca gtttttcagg
     1441 atgaaattag gacaattagc gtacctaacg actaatcgtc attgtttccg ccaccgaaaa
     1501 gcaagagcag acggagcagt tgcaagaaac tcatgaaggc agctgcgaca tacgtcatgg
     1561 cagctgcaac aagaactttg cgtgctttcg gcacttcttg tgcattcagg atgccaccat
     1621 tcgaaagaat tctcaaagca cgcccggatg cattgaattc gactggcaat g
//
LOCUS       DAOMUJ010000423         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20567_length_1651_cov_1.985589, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000423 DAOMUJ010000000
VERSION     DAOMUJ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20567_length_1651_cov_1.985589"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..359)
                     /locus_tag="PLT73_08980"
     CDS             complement(159..359)
                     /locus_tag="PLT73_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRM20541.1"
                     /translation="MEKGTVKWFNAEKGFGFIERESGDDVFVHFSAILGDGFKSLEEG
                     QAVSFEIVEGNRGAQAANVEKI"
     gene            complement(550..1239)
                     /gene="radC"
                     /locus_tag="PLT73_08985"
     CDS             complement(550..1239)
                     /gene="radC"
                     /locus_tag="PLT73_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRM20542.1"
                     /translation="MVQETNLLMEVPKASRPRERLDQFGEKALATHELLAIVLRTGPR
                     DSNVMQLALRVLNEFEDLHSLKMASLEELTAIHGIGRTKAIEIKACVELGVRVANATQ
                     LKSGTITSTQSAGTLLQKEMRDLQQEHVVAVYLNTKNEIIKKKTIFIGSLNSSVAHPR
                     EIFREAVRFAAARIILAHNHPSGNPDPSEADLVFTRRMVECGEMMGIEILDHFIIGVD
                     DYLSLREYGII"
     gene            complement(1299..>1651)
                     /locus_tag="PLT73_08990"
     CDS             complement(1299..>1651)
                     /locus_tag="PLT73_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM20543.1"
                     /translation="EGVICVVASSSWRKEVSFYLELADILQYFRYQVCGDEVSFAKPN
                     PEIFLSAWKPLGIPKEDCLILEDSLNGIRAAYDAGIEVIMVPDLIVPDDEAKAKTIAI
                     LPDLHAVMDWLKEK"
BASE COUNT          403 a          427 c          345 g          476 t
ORIGIN      
        1 aagctggaca tcattttaga tgcatgaaac atgttgcaaa cttgtgaaac acctatggtg
       61 atgcactttt gagttgaaaa aattttataa attcctatat gtcttaaaaa ggcccgagac
      121 agtgtcccgg gccttttgat cgattaagtt aagattaatt agattttttc aacgttggca
      181 gcttgtgctc cacgatttcc ctcaacgatt tcgaatgaaa cggcttgtcc ttcttccaat
      241 gacttgaatc cgtcgcctaa gatagctgag aaatgtacga atacgtcatc tccgctttcg
      301 cgttcgataa atccgaagcc tttttctgcg ttaaaccatt ttactgttcc tttttccatg
      361 aaaaattcct cctcgtgcaa agcactttaa attgttattc ttgcttttgg tacgatagga
      421 atgttcatga caatctattc tttaccaaac aaaaacacta acataatgat atatcaggtt
      481 ttccgatatt gcaagttttt tgccatcaaa ttttcatttc agcttgcgta aatcgtttag
      541 gaacctttac tatatgatac cgtattcccg caggctgaga taatcatcga ccccgatgat
      601 gaaatgatca aggatctcga tccccatcat ttctccgcac tccaccatcc tgcgggtaaa
      661 caccaggtcg gcttccgagg gatcgggatt accggaagga tgattatgcg caagaatgat
      721 ccgcgccgcc gcaaacctta ccgcctcccg gaatatctcg cggggatgcg caaccgaact
      781 gttcaggctg ccgatgaaga tcgtcttctt cttgatgatt tcgttctttg tgttcagata
      841 gacagccacc acgtgctcct gctggagatc gcgcatctct ttctgcagca gcgtcccggc
      901 gctttgcgtc gatgtgatcg ttccgctctt cagttgcgtg gcattggcta cacgcacccc
      961 caattcaaca caggccttga tttcgatcgc tttggttcgg ccgattccat gaatagcagt
     1021 caattcttca agcgaggcca tcttcaagga atgcagatcc tcgaactcat tcagcacccg
     1081 caacgccagc tgcatgacgt tgctgtccct gggccctgtc cgcagaacga tagccaataa
     1141 ttcatgcgtc gccaaggctt tttctccgaa ttgatccaat ctttcgcgcg gtcgcgaggc
     1201 ttttggaacc tccatcagca gatttgtttc ttgcaccatc ctgttttacc cctttccttc
     1261 cgtctactca ataggtacgc aggaaggagc ctaacaggtt atttctcttt cagccaatcc
     1321 atgaccgcat gcaggtcggg caaaatggca attgtttttg ctttggcttc gtcatcggga
     1381 acgatcagat ccggaaccat gatcacttca atgcccgcat cgtaggcagc acggatgcca
     1441 ttaagcgaat cttccaggat cagacagtcc tctttcggaa tgccaagggg tttccaggcg
     1501 ctcaggaaaa tttccggatt cggtttggcg aaggatactt catccccgca cacttggtag
     1561 cggaaatact gtaaaatatc agccaattcg aggtagaaac tgacttcttt cctccagctg
     1621 ctggaagcaa cgacgcatat tacgccttcc t
//
LOCUS       DAOMUJ010000424         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20682_length_1644_cov_1.874764, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000424 DAOMUJ010000000
VERSION     DAOMUJ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_1644_cov_1.874764"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLT73_08995"
     CDS             <1..666
                     /locus_tag="PLT73_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRM20544.1"
                     /translation="KMSLTPLDIQHKEFPVKIKGYDKEQVNDFLDNVTKEFEEIIRHN
                     KDLQKQLKFAEEKLQYFSNLQDALNKSIVVAQDAADRLKENARKEAEIILFEAEKSAD
                     RLLHEAAGKATKINEETDGVRKESRNFKQKLQLLVESQLNLIMNDEWNNLLNASPEGQ
                     VSTPTLNEVLSNRTRIIDELVANSGDAAEFEVGGRLAEEASAEEKLAEVAVEAIEIPQ
                     ENK"
     gene            958..>1644
                     /locus_tag="PLT73_09000"
     CDS             958..>1644
                     /locus_tag="PLT73_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM20545.1"
                     /translation="MKMKDTLHLGKTAFPMKANLPVRELEWQKEWEEKDIYVKRQEKN
                     ADKPTFVLHDGPPYANGAVHMGHALNKISKDIIVRSKSMSGFRAPFVPGWDTHGLPIE
                     QALTNTGVNRKAMSLADFRKLCEDYAWKQIDGQRTVFKRLGIAGDWENPYVTLLPKYE
                     EAEIRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIYVAFQVKDGK
                     GLLANDTAFII"
BASE COUNT          540 a          337 c          388 g          379 t
ORIGIN      
        1 aagatgagtt tgactccatt agatatccaa cacaaggaat tccctgtcaa aataaaaggg
       61 tacgataaag aacaagtcaa tgactttttg gataatgtca caaaagaatt tgaagaaatc
      121 atcagacaca ataaagatct gcaaaagcaa ctgaaatttg ccgaagaaaa gcttcagtat
      181 ttcagcaacc tgcaggatgc tttgaacaag tcgattgttg tggcgcagga tgctgccgac
      241 agactgaaag aaaatgcgcg caaagaagct gaaatcattt tattcgaggc ggaaaaaagt
      301 gcggatcgct tattgcatga agcggctggg aaagcaacaa aaatcaatga agagactgac
      361 ggcgtccgca aggaaagccg caacttcaaa cagaaactgc aacttttggt tgagtcgcaa
      421 ttgaacctca tcatgaatga tgaatggaat aatctgttga atgcttcacc ggaagggcaa
      481 gtttcaacac ctactttgaa tgaagtgttg tccaaccgca cacgcatcat cgatgaattg
      541 gtcgccaatt ccggtgacgc agccgagttc gaagtcggcg gaagattggc cgaagaagcc
      601 agcgccgaag agaaacttgc tgaagttgct gtggaagcta ttgaaattcc gcaagaaaac
      661 aagtaaaatc aatgtattga agaatggaac acccgaagaa aagacacttc cagcgaattg
      721 gtaaacggtg aaaggccaat aagtccctct ttttttagga tcctccatga gaatcttttt
      781 gaacaaacat aagtagaaaa ggtcgcctga acagcgttaa cggtttgaat gaggaagcat
      841 gacagcctgt tccggccgtt gtcgctttaa atttgggtgg taccacgaag acttcgtccc
      901 ttttggagac gaagtctttt tttgtagaaa aaaataatca aaagggagct tccgacaatg
      961 aaaatgaaag atacgctgca cctggggaaa acagcttttc ctatgaaagc caacctgcct
     1021 gtccgtgaat tggaatggca aaaggaatgg gaagaaaaag atatttatgt aaaacgacaa
     1081 gaaaaaaacg cggataaacc gacttttgtg cttcacgatg gccctccgta cgccaacggt
     1141 gctgtccata tgggacatgc cttgaacaaa atcagcaagg acatcatcgt ccgctcgaaa
     1201 tccatgtctg gcttccgcgc gccttttgtc cctggttggg atacgcatgg tttaccgatc
     1261 gaacaagcct taacgaatac aggtgtgaac cgcaaagcga tgagcttggc tgatttccgt
     1321 aaattgtgtg aagattatgc ttggaaacag atcgacggac aaagaaccgt cttcaaacgc
     1381 ttgggcatcg ctggtgactg ggaaaaccca tatgtgacgc tgttgccgaa atatgaagaa
     1441 gctgaaatcc gtgtattcgg aaaaatggca gaaaaaggct acatctacaa aggcttgaag
     1501 ccaatctatt ggtcgccatc gagtgaatcc tcgcttgcgg aagccgaaat cgaatataaa
     1561 gatgtaaaat ctccttcaat ctacgtggct ttccaagtca aagacggcaa aggccttctt
     1621 gctaacgata ctgcgttcat catc
//
LOCUS       DAOMUJ010000425         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20726_length_1642_cov_1.810964, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000425 DAOMUJ010000000
VERSION     DAOMUJ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20726_length_1642_cov_1.810964"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..484
                     /locus_tag="PLT73_09005"
     CDS             86..484
                     /locus_tag="PLT73_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM20546.1"
                     /translation="MSGQRIVPFLTLPGTAEAAMHFYVSIFPNSSVTSLTKIDEYEHG
                     EGGRVINEQFVLNGQEVMAMDMEEDFLPDMTWAVSLYVDCEDEASFDNYFDSLSADGV
                     VMMGPEAVGELRAVAWVTDQFGVTWQLVWK"
     gene            complement(569..973)
                     /locus_tag="PLT73_09010"
     CDS             complement(569..973)
                     /locus_tag="PLT73_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM20547.1"
                     /translation="MPKKQKKLSFLLFLSGLLLVACGKEGDSQIVAINNETEYEEVVA
                     QDVAYLYFGFDDCPYCKEFRPILEEELAETEQTAYYYNTKKRAKDVNYDEVLDTYGIA
                     FVPILIKLEDGKAVGSVNLDTVADLPMLLAEE"
     gene            complement(1084..>1642)
                     /locus_tag="PLT73_09015"
     CDS             complement(1084..>1642)
                     /locus_tag="PLT73_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696404.1"
                     /note="similar to YegS from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRM20548.1"
                     /translation="YLAYLVKGAEKIPQLKPMHMRIEYEGGVYDGMASMFFVALTNSV
                     GGFETIDPNIVLGDGKFTLFIVKTANIFEILQLLAQVLNNGRHLENSNVLYTHTSFVR
                     AESMDGSRLMINLDGEYGGDAPTLFTNHQQHIKIIGNTSDYADPIEEVDEGILEGAGT
                     GFMKEVETLHPDEVNDKRLPYDGKN"
BASE COUNT          394 a          396 c          392 g          460 t
ORIGIN      
        1 gataggaagt tccaacctta ttcagtataa tggaagtata cagaccagtt gctgatgggt
       61 acaggaagaa agcgaggatg acaaaatgag cggacaacgg attgttccct ttttgacgtt
      121 gccgggaact gcagaagcag cgatgcactt ttatgtttcc atttttccga attccagcgt
      181 gacctccctc accaaaatcg atgagtatga gcatggggaa ggcggtcgcg taatcaatga
      241 gcagtttgtt ttgaatggac aggaagtcat ggcgatggac atggaagagg atttcttgcc
      301 ggatatgact tgggccgttt ccctctacgt ggactgcgaa gatgaagcca gcttcgacaa
      361 ctactttgat tccttatccg cagatggcgt tgtgatgatg ggacctgaag cagtcggcga
      421 attgcgcgcg gtagcttggg ttaccgacca attcggtgtt acttggcaat tagtgtggaa
      481 ataatgcgaa tgggctgctc cagttttttg ggggcagccc attctttatg gttatcggac
      541 attctcgggc aaccacactc catactcgct attcttccgc caataacatt ggcaagtcgg
      601 ctacggtgtc cagattgaca gaaccgaccg cttttccgtc ctccagcttg atcaggatgg
      661 ggacgaacgc gatgccgtat gtatccagca cctcgtcata gttgacgtcc ttggcgcgtt
      721 ttttcgtatt gtagtaatag gcggtctgct ctgtttccgc caattcctct tccaggatcg
      781 gacggaattc cttacagtag ggacagtcat cgaatccgaa gtagaggtag gccacatcct
      841 gcgcgaccac ttcttcgtat tccgtttcat tgttgatcgc tacaatctgg gaatccccct
      901 ctttcccgca ggctacaagc agcagtccac tcaagaataa taggaacgat aatttcttct
      961 gttttttcgg catacggcaa gtccccttta gcataaacgt ttcttttttc cagtataaca
     1021 aaaaatgccc ctgacctctc atcataagac cgagaggccg ggcattcttt ctgttttcaa
     1081 tttttagttt ttaccgtcgt aaggcaagcg tttgtcgttc acttcatccg ggtgcaaggt
     1141 ttcaacttcc ttcatgaagc ctgtgccggc gccttccaga atgccttcat cgacttcttc
     1201 aatcggatca gcgtaatcgg aagtgtttcc gatgattttg atgtgttgct ggtggtttgt
     1261 gaacagagtc ggtgcatccc cgccatactc accatccaag ttgatcatca agcgtgaacc
     1321 atccatggat tccgctctca caaagctagt atgtgtgtag aggacattcg aattttcgag
     1381 atgtctgcca ttgttcagta cctgggccaa cagttgcagg atttcaaaaa tattagcagt
     1441 ctttacgata aacagagtga acttgccgtc acccaaaacg atgttcggat cgatcgtctc
     1501 aaagccgccc acggagtttg tcaaagctac aaagaacatc gaagccatac cgtcatatac
     1561 cccgccctcg tattctatgc gcatatgcat cggtttgagt tgcgggattt tttcggcacc
     1621 cttcaccaaa taggcaaggt ag
//
LOCUS       DAOMUJ010000426         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20727_length_1642_cov_1.722747, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000426 DAOMUJ010000000
VERSION     DAOMUJ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20727_length_1642_cov_1.722747"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLT73_09020"
     CDS             complement(<1..312)
                     /locus_tag="PLT73_09020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM20549.1"
                     /translation="MKQKLIALDLDGTTLNNQSLLSVRTKNVLARLQKDNHLVMIATG
                     RPYRNSKEFYETLKMTTPLVNFNGALCHNPTDSNWRNYYHKTLNKDFAMDLIEQREEM
                     GI"
     gene            complement(353..787)
                     /locus_tag="PLT73_09025"
     CDS             complement(353..787)
                     /locus_tag="PLT73_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20550.1"
                     /translation="MTILAALDQKFQEKGMPLKSHPIQNGQYLYQGKFNLDKDHSLPF
                     GVVIAKDEAESDFQISYRKLAYLNNYADKVKLLELINELNQTKTFYYTLCLAGDGEIF
                     LRLLGRSTTDVKPLYEMLVIGSNIAKVMTAELKSKFPSLGSE"
     gene            complement(914..1267)
                     /gene="rplS"
                     /locus_tag="PLT73_09030"
     CDS             complement(914..1267)
                     /gene="rplS"
                     /locus_tag="PLT73_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM20551.1"
                     /translation="MSQLPLIEAITQGQLRSDIPAFRPGDTVRVHARVVEGTRERIQL
                     FEGVVIKRRGFGISETYTVRKISNGVGVERTFPLHTPRVAQIEVVRFGKVRRAKLYYL
                     RALHGKAARIREKRR"
     gene            complement(1434..>1642)
                     /locus_tag="PLT73_09035"
     CDS             complement(1434..>1642)
                     /locus_tag="PLT73_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRM20552.1"
                     /translation="YTRPRDFRGDVVPEVLFSGNHQKIADWQEKESLRKTWLRRPEML
                     EKITLSKKQQTWLAEIFLEEKADN"
BASE COUNT          407 a          373 c          334 g          528 t
ORIGIN      
        1 gatgcccatc tcttcacgct gttcgatgag atccatcgcg aaatctttat tgagtgtttt
       61 atggtagtaa ttacgccaat tactgtcggt tggattatgg catagggcac cgttgaaatt
      121 caccaacggg gtagtcatct tgagtgtctc ataaaattct ttgctgtttc tgtatggacg
      181 accagttgcg atcatgacca gatggttatc tttttgcaac cgagccaaca catttttggt
      241 ccgaacgctc agcaacgact gattgttgag cgtggtaccg tctagatcta aagcgattaa
      301 tttttgtttc atatttcctc ctgcagtttc cgcctgacat tcgagccctt acttattcgc
      361 ttcccagcga cggaaattta ctttttaatt ctgccgtcat gacctttgcg atgttcgatc
      421 cgatcacaag catttcatat agtggtttga catctgttgt cgagcggccc aaaagccgaa
      481 ggaaaatctc tccatcgccc gccaggcaaa gtgtataata aaaagtcttg gtttggttca
      541 attcattgat cagttccaaa agcttcactt tatcagcata attattcaga taagctaatt
      601 ttctgtagct gatctgaaaa tcactttctg cctcatcttt ggcgatgact acaccgaacg
      661 gcagagaatg gtccttatcg agattgaatt tcccttgata caaatactgg ccattttgga
      721 tgggatgact tttcagtggc atgccttttt cttggaattt ttgatcaagc gcggctaata
      781 tcgtcaaaac tatcactctt ttcgtatatg gtgctacaag tatagcacat ttcccccaag
      841 aattatgctt atcgcactat acaaaaaagg tccggaaccg aagtccagac ctttccatat
      901 gtcatgcggg atattaacga cgtttttctc tgatacgagc agcttttccg tgtaatgcac
      961 gcaagtagta aagttttgca cgacgcactt taccgaagcg aacaacttcg atttgagcta
     1021 cacgaggagt gtgcaatggg aatgtacgtt ccacaccgac accgttggaa attttacgta
     1081 ctgtatacgt ttcgctgatt ccgaatccgc gacgtttgat gactacgcct tcgaataatt
     1141 ggatacgttc tcttgttcct tcgactacgc gagcgtggac gcgaacagtg tctccaggac
     1201 ggaatgcagg gatatcacta cgtaattgtc cttgtgttat tgcttcgatc aatggtaatt
     1261 gactcatgtt tttttctcct tccaaccgac attcatacac aatccttctg cacagcggaa
     1321 tatcgtatta tggagcagtc tctctgctca cctgtagaat tctaccacaa tatttttgac
     1381 gtgtaaagta tttctcgttt acattgttta aatttcaagg cgcacgattg cgcttaattg
     1441 tcagcttttt cttcgagaaa aatttcagcc aaccaggttt gctgtttttt cgacaaggtg
     1501 atcttttcca gcatttccgg acgtctcagc caagttttgc gcaaagattc tttttcttgc
     1561 caatcagcga ttttctggtg attgccgctg aaaagcactt cagggacgac atctccccgg
     1621 aaatcgcggg gtctcgtata tt
//
LOCUS       DAOMUJ010000427         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20745_length_1641_cov_1.743380, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000427 DAOMUJ010000000
VERSION     DAOMUJ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20745_length_1641_cov_1.743380"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..885)
                     /locus_tag="PLT73_09040"
     CDS             complement(100..885)
                     /locus_tag="PLT73_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM20553.1"
                     /translation="MDFQNRVVVVTGGANGIGRATVESFLAAGAAVAFVDTDKEAGQK
                     IVSRFATNDVYFHHGDIAEEAALHAFAEAVKQRFGKVDYLVNNACISKKGILSACSFQ
                     DFNYVLALGVTAPYLLTSLFLPVFTAGASVVNIASSRGFMSQKDTESYSAAKGGILAL
                     THALSISLAGKARVNSISPGWIDTGSFHKEEDYQPDYEAADLLQHPVGRVGVPDDIVQ
                     MIRFLCSDAAGFITGQNFTVDGGMSKLMIYHNDHGWTYNPEAE"
     gene            complement(929..>1641)
                     /locus_tag="PLT73_09045"
     CDS             complement(929..>1641)
                     /locus_tag="PLT73_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM20554.1"
                     /translation="NKNFADIFSDFDADFFCLQETKLQEGQIELAFPGYESYWNYADR
                     KGYSGTAIYTKHTPLSVRLGISVAEHDSEGRVITLEYGDFFLVNVYTPNSQEQLKRLD
                     YRMHWETAFLAYLKGLETEKPVIVCGDLNVAHENIDLKNWKTNRKNAGFSDEERAKFS
                     DVVANGFIDTFRYFHPDAEGRYSWWSYRFNARKNNAGWRIDYFLVSEALKDALVDADI
                     HENIFGSDHCPVSLKLSL"
BASE COUNT          378 a          430 c          427 g          406 t
ORIGIN      
        1 gggccggata ataaaaataa gcaggatacc tttcatatga aaggtatcct gcttattttt
       61 gtgggtagtt cagcatgcgc gttcacccaa cacggccgat cattcggctt cgggattata
      121 ggtccagcca tggtcgttgt ggtagatcat gagcttggac atcccgccat cgacagtgaa
      181 attctgcccg gtgatgaagc cggctgcgtc gctgcagagg aaacggatca tctgtacgat
      241 atcatccggc acaccgacac ggccgacggg atgctgcagg agatccgcgg cttcatagtc
      301 cggttgatag tcttcctctt tatggaagga acccgtgtcg atccaacctg ggctgatgga
      361 attgacccgg gctttcccgg ccaatgagat actgagggcg tgcgtcaacg cgaggatgcc
      421 gcctttagcc gccgagtaac tttcggtgtc cttttgcgac atgaaaccgc ggcttgatgc
      481 gatgttcacg accgaggcgc cagctgtgaa caccggcaaa aacaagctcg ttaacaggta
      541 cggagctgtg acgcccaaag ccaggacata attgaagtcc tgaaaggaac acgccgacag
      601 gattcctttt ttgctgatgc aggcgttgtt caccagataa tcgactttgc cgaagcgttg
      661 cttgacggct tccgcaaaag cgtgaagggc agcctcctca gcgatgtctc cgtgatggaa
      721 atagacatcg ttcgttgcga aacgggaaac gatcttttgg ccggcttcct tatccgtgtc
      781 cacgaatgcg acagcggcac ccgcagccaa aaatgattcc accgtggctc ggccgatgcc
      841 gtttgctccg ccggtcacca cgactacacg attttgaaaa tccatattcc attcctcccg
      901 actgattgat gcttctgata tttcgttgct acagcgaaag cttcagcgat accgggcaat
      961 ggtcgcttcc gaagatattt tcatggatat ccgcgtcgac cagagcatcc ttcaaagctt
     1021 ccgataccag gaagtagtcg atccgccaac ctgcattgtt tttgcgggcg ttgaagcggt
     1081 aactccacca ggaatatctg ccttccgcat ccggatggaa gtagcggaaa gtatcgatga
     1141 atccgttcgc gaccacatcg ctgaacttgg cgcgttcctc gtccgaaaag cccgcgtttt
     1201 tgcggttcgt tttccaattc ttcagatcga tgttttcgtg ggcaacgttg aggtcaccgc
     1261 agacaatgac cggtttttct gtctctaggc ccttcagata agccagaaag gccgtttccc
     1321 aatgcatccg gtagtccaac cgcttcagct gctcctgtga attcggggtg tagacgttga
     1381 ccaggaagaa atcgccatac tccaaggtga tgacgcgtcc ttccgaatca tgttcggcaa
     1441 cgctgatgcc taatcggaca gagagcgggg tgtgtttcgt gtatatcgcg gttcctgaat
     1501 agcctttgcg atcggcataa ttccagtagg attcatatcc gggaaatgct agctcgatct
     1561 gtccttcctg cagttttgtt tcctgcaggc agaagaaatc agcatcgaag tccgaaaaaa
     1621 tatccgcaaa gttcttgttt a
//
LOCUS       DAOMUJ010000428         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20746_length_1641_cov_1.719420, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000428 DAOMUJ010000000
VERSION     DAOMUJ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20746_length_1641_cov_1.719420"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..>1641)
                     /gene="groL"
                     /locus_tag="PLT73_09050"
     CDS             complement(36..>1641)
                     /gene="groL"
                     /locus_tag="PLT73_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRM20555.1"
                     /translation="KFSEDARAAMLRGVDILANTVKVTLGPKGRNVVLEKAYGSPLIT
                     NDGVTIAKEIELEDRFENMGAKLVAEVASKTNDIAGDGTTTATVLTQAIVREGLKNVT
                     AGANPVGIRRGIELATQAAVKGLAEISQTVNSRESIAQVAAVSSGSKEIGELIAEAME
                     KVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTNNDKMEAELEAPYILITDR
                     KLSNIQDILPLLEQILQQGRPLLIVADDVDGEALPTLVLNKLRGTFNVAAVKAPGFGD
                     RRKEMLQDIAILTGGTVIAEDLGLELKDTTVEHLGRAGKVVVTKDSTTIVEGAGEKEA
                     IEQRVAVIRAQSAETTSEFDREKLQERLAKLSGGVGVIKVGAATETELKERKLRIEDA
                     LNATRAAVEEGIVSGGGTALINVQKRVEALELTGDEATGARIVARSLEEPVRQIAENA
                     GKEGSVVADKIKGLEVGMGYNAATDEWVNMIEAGIVDPTKVTRSALQNAASVAALILS
                     TEAIVADRPAPATAPAPAMDPGMGMM"
BASE COUNT          358 a          443 c          358 g          482 t
ORIGIN      
        1 aaactctgag ctcaggattc atcacacact gttttttaca tcatgcccat gcctgggtcc
       61 atggctgggg ctggggctgt ggctggggct ggacggtcgg ctacgatcgc ttctgtcgat
      121 aggatcaggg cagctacgct ggctgcgttt tgtaaagcgg aacgtgtcac ttttgtagga
      181 tccacgattc ctgcttctat catgttcacc cactcatcgg ttgcggcgtt ataacccata
      241 ccaacttcaa gtcctttgat cttatcggct acaacagagc cttctttgcc agcattttca
      301 gcgatttgtc tcactggctc ttccaatgaa cgcgctacga tgcgcgcacc tgttgcttcg
      361 tcgccggtca gttccaacgc ttcaacgcgt ttttgaacgt tgatcaaagc tgtaccacca
      421 ccggatacga taccttcttc aacagcggcg cgggttgcat tcaaagcatc ttcgatacgc
      481 agtttgcgtt ctttcaattc agtttcggta gcagctccga ctttgatgac gccgacgcca
      541 ccggataatt tcgcaagacg ttcctgtaat ttctcacggt caaactctga agttgtctcg
      601 gctgattgcg cacggatgac cgcaacacgc tgttcgatgg cttctttttc gccggctcct
      661 tcaacgatgg ttgtggaatc tttggttacg acaactttac ctgcacgacc caaatgttct
      721 acggtagtat ccttcaattc caagcctaaa tcctcagcaa taacggtacc acctgtcagg
      781 attgcgatgt cctgcagcat ttctttacga cggtcaccga aaccaggtgc tttgactgcc
      841 gctacattga atgttccgcg caatttgttc aatactaatg taggcaacgc ttcgccgtca
      901 acatcatcag caacgatcaa caacgggcgt ccttgttgca gaatctgttc caataaaggc
      961 aagatgtctt gaatattgga caatttacga tccgtgatca ggatataagg tgcttccaat
     1021 tctgcttcca ttttgtcgtt gttggtgacc atgtattgcg ataggtaacc gcggtcgaat
     1081 tgcatccctt caacaacatc caactcggtt tcgatccctt ttgactcttc gatcgtgatg
     1141 actccgtcgt tgccgacttt ctccatcgct tccgcaatca attcgccgat ttctttcgat
     1201 ccggacgata cagcagcaac ttgcgcgatc gattctcttg aattcacggt ttgggaaatt
     1261 tccgccaaac cttttacagc tgcttgtgta gccaactcga taccgcgacg gatgcccact
     1321 gggtttgcgc ctgcagtaac gtttttcagc ccttcgcgaa cgatggcttg agtcaaaaca
     1381 gtagcagtcg ttgtaccgtc accggcaata tcgtttgttt tggaagcaac ttcggcaacc
     1441 agtttcgctc ccatattttc gaagcgatct tccaattcga tttctttggc gatcgtcaca
     1501 ccatcattcg tgatcaacgg agaaccgtac gctttttcca aaacgacgtt gcgtcctttg
     1561 ggtcctaaag tcacttttac ggtatttgct aagatatcca caccacgaag cattgctgct
     1621 cttgcgtctt ctgaaaattt a
//
LOCUS       DAOMUJ010000429         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20756_length_1640_cov_2.257413, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000429 DAOMUJ010000000
VERSION     DAOMUJ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20756_length_1640_cov_2.257413"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="ribD"
                     /locus_tag="PLT73_09055"
     CDS             <1..666
                     /gene="ribD"
                     /locus_tag="PLT73_09055"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRM20556.1"
                     /translation="KEPFVVMKAAMSLDGKIASRTGESQWITGPAARERVHQLRSALT
                     GIMVGVQTVIMDDPQLTSRIPGGKNPLRIIVDSTLRIPLEAKVLQDQDVAKTLIATTE
                     WADRNKAALLEAAGIELLTVPTKDGRTDLKALMKVLGERGIDSILLEGGATLNFATLQ
                     AGIVDKVQVYIAPKLIGGETAKTPVGGEGIEKLSQAFSVIELKASTVGEDILLEGYLS
                     KNE"
     gene            690..1343
                     /locus_tag="PLT73_09060"
     CDS             690..1343
                     /locus_tag="PLT73_09060"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRM20557.1"
                     /translation="MFTGIIEEVGTLKGIRHGQKSSVVTIGGKKVLIGTKIGDSISTN
                     GVCLTVTKLHKDAFDADVMPETLQRSNLGGQQMGGRVNLERALQLDTRLGGHIVSGHI
                     DGTGSIKEYRKDDNAVWITISAGPELLRYIIEKGSIAIDGVSLTVATVDSGSFQVSII
                     PHTGAETILLEKKLGDTVNLECDIIGKYVEKLLGLSPKTQTKQSNLSEAFLQENGFF"
     gene            1366..>1640
                     /locus_tag="PLT73_09065"
     CDS             1366..>1640
                     /locus_tag="PLT73_09065"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRM20558.1"
                     /translation="MFNTSEEIIADIKAGKIIVLVDDEDRENEGDLICAAEFATPENI
                     NFMAIYAKGLICMPMDRTLTEKLVLTPMVDRNTDNHGTAFTVAIDHV"
BASE COUNT          453 a          449 c          439 g          299 t
ORIGIN      
        1 aaagaaccct tcgtcgtcat gaaagcagcg atgtccctgg acggaaagat cgcgtcccga
       61 accggcgaat cgcaatggat caccggacct gccgccaggg aacgcgtgca ccaactgcgc
      121 agcgccctca ccggcatcat ggtcggtgtc cagaccgtca tcatggacga tccgcaactg
      181 acttcccgca ttccgggagg aaaaaatccg ctgcggatca tcgtcgacag caccttgcgc
      241 atccctttgg aagcaaaagt gctccaagat caggatgtgg ccaagactct aatcgcaacg
      301 acagaatggg cggaccgcaa taaagccgca ctgctcgaag cagccggcat cgagcttttg
      361 actgtaccga ccaaggatgg ccggaccgat ctgaaggcgc tgatgaaagt tctcggagag
      421 cgcggcatcg acagcatcct cttggaaggc ggcgccactt tgaactttgc cacattgcaa
      481 gcaggcatcg tcgacaaggt gcaagtctac atcgccccga aactgatcgg cggggaaacg
      541 gcaaaaacac ctgtcggcgg agaaggtatc gagaagctga gccaagcttt cagcgtcatc
      601 gagttgaagg ccagcaccgt tggggaagat atcctcttgg aaggctacct ttccaagaac
      661 gaataataga ggaaagtggg aggacattca tgtttactgg aatcatcgaa gaagtcggca
      721 ccttaaaagg catccgtcat ggccaaaaat cttccgtcgt cactatcggc ggaaaaaaag
      781 tgctgatcgg aacaaaaatc ggcgacagca tctccaccaa cggcgtctgc ctgacggtaa
      841 cgaagctgca taaggatgcc ttcgatgccg acgtcatgcc ggaaacgctg caacgctcaa
      901 accttggcgg ccagcagatg ggcggtcgcg tcaatctgga acgggcattg cagcttgata
      961 cccgcctggg cgggcacatc gtcagcggcc acatcgatgg caccggatcg atcaaggaat
     1021 accgcaagga cgataatgcc gtctggatca ccatatcagc cggccccgaa ctgctccgct
     1081 acatcatcga aaaaggctcg atcgccatcg acggcgtcag tctgactgtg gcgacggtcg
     1141 acagcggctc gttccaggta tccatcattc cccataccgg agcggagacg atcctgctcg
     1201 agaagaaatt gggcgacacg gtcaatctgg agtgcgacat catcggaaaa tacgtggaaa
     1261 aactgctggg cctgagccct aaaacacaaa caaaacaaag caacctatct gaagctttct
     1321 tgcaagagaa cggctttttc tgaaattaag aggagggaaa catacatgtt caatacatct
     1381 gaagaaatca tcgcggacat caaagccggc aaaatcatcg tgcttgtcga tgatgaggat
     1441 cgcgaaaatg aaggcgatct gatctgcgca gcggaattcg cgactccgga aaacatcaat
     1501 ttcatggcca tctacgcgaa aggcttaatc tgtatgccga tggatcggac actcaccgag
     1561 aaactggtac tgactccgat ggtggaccgc aacaccgata atcatgggac agccttcacc
     1621 gtcgccatcg accatgtcga
//
LOCUS       DAOMUJ010000430         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20775_length_1639_cov_2.494949, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000430 DAOMUJ010000000
VERSION     DAOMUJ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20775_length_1639_cov_2.494949"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLT73_09070"
     CDS             <1..691
                     /locus_tag="PLT73_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM20559.1"
                     /translation="ENVLEFIRKQMDNGQQAYIITPLIEKSESLDVKNAEDVHQAVTA
                     YYKGKYQIGLLHGRLKSEEKDQIMRSFQNNELQILVSTTVIEVGLNVPNATVMVIQDA
                     DRFGLSQLHQLRGRVGRGDKESYCVLVADPKTDNGKERMRIMVESTDGFYLSQRDLEL
                     RGPGDLFGNKQSGLPDFKSGDIVRDAVILDIAHNDAIQITREPDFESNPNYLSLREAV
                     EREKEKLKRLQ"
     gene            719..>1639
                     /gene="plsX"
                     /locus_tag="PLT73_09075"
     CDS             719..>1639
                     /gene="plsX"
                     /locus_tag="PLT73_09075"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRM20560.1"
                     /translation="MKIAVDAMGGDFAPQAIVEGILMASKEFNDLEFLLFGDEAKIRK
                     ALGDQYAEGGKITIVHTTEKIESDDEPVKAIRRKKQASMVLAAQAVKNKEADALFSAG
                     NTGALLAAGLLIIGRIKGIDRPGLMPILPVIGKENGQFLLMDAGANADCKPENIYQFG
                     LLGSYYAKFVQGIENPRIALLNNGAEATKGSELSKKAYVLLEEDSELNFTGNIEAREI
                     LLGAADVVVTDGFTGNAVLKTMEGTAMSLLKLIKTQLLSGGLKTKIGGMLIKDSFSEI
                     KDTMDYSKHGGAVLFGLRAPVVKTHGSADKV"
BASE COUNT          486 a          350 c          438 g          365 t
ORIGIN      
        1 cgaaaatgta cttgagttca ttcgaaaaca gatggataat ggacaacagg cgtacatcat
       61 cacgcctctg atcgagaaat ccgaatcgct ggatgtcaag aatgcggaag atgtgcatca
      121 agccgtgacg gcctattaca aaggaaagta ccaaatcggt ctgttgcacg gccgtctcaa
      181 gtccgaagaa aaggatcaga tcatgcgcag tttccaaaac aacgagctgc agatcctggt
      241 atcgacaaca gtcatcgagg tcggcctaaa cgtgccgaat gcgacagtga tggtcatcca
      301 agatgccgac cgattcggat tatcgcaact ccaccaattg cgcggtcgag tgggccgggg
      361 agacaaagaa tcctattgcg tgctggttgc cgatccgaaa accgataacg gcaaggagcg
      421 catgcgcatc atggtggagt cgactgacgg tttctacctg agccaacggg atttggaact
      481 gcgcggaccg ggtgatcttt tcggaaacaa acaatcaggc ttgcccgatt tcaaatccgg
      541 agatattgta cgtgatgccg tcatcctgga tattgcgcat aatgacgcta tccaaatcac
      601 acgcgaacca gactttgaaa gcaatccaaa ctacttaagt ttgcgggaag cagtcgaacg
      661 cgaaaaagag aagttgaaga gactacagtg aaactaacaa aagtgaggta tacgatttat
      721 gaaaatcgct gtcgatgcga tgggcgggga ttttgcgcca caagcaatag tagagggcat
      781 tttaatggcc agcaaagaat ttaatgatct ggagtttttg ctttttggcg acgaagccaa
      841 aatacgtaag gctttagggg atcaatatgc cgaaggtgga aaaatcacaa tcgtgcatac
      901 gaccgaaaag atcgagagcg atgacgaacc cgtcaaagca atccgtagaa aaaaacaagc
      961 ttcgatggtg cttgctgctc aagcagtcaa gaacaaagag gccgacgctt tattttcggc
     1021 agggaatacc ggtgcgcttt tggcagccgg attattgatc atcggccgca tcaaagggat
     1081 cgacagacct ggcttgatgc cgattctgcc agtcatcggg aaagaaaatg gacaattcct
     1141 tttgatggat gctggcgcaa acgctgactg caaaccggag aatatctacc agttcggcct
     1201 tttgggctcg tattatgcca agtttgtgca aggcatcgaa aatccaagaa tcgctttgtt
     1261 gaacaatggt gccgaagcaa cgaagggcag cgaactatcc aaaaaagcct atgtattgtt
     1321 ggaagaggac tctgaactca atttcacagg taatatcgag gcacgggaaa ttttgcttgg
     1381 cgctgcggat gttgtcgtga cggacggatt tacagggaat gcagttctaa aaacaatgga
     1441 aggtacggca atgtccttgc tgaagttgat caaaacccaa ttgctttcag gcggtctgaa
     1501 gactaaaatc ggcggtatgc tgatcaagga ttccttttcg gagatcaaag atacgatgga
     1561 ttattcgaag cacggaggag ctgtgctgtt cggattgcgg gcgcctgtcg tcaagacaca
     1621 tggatctgcg gacaaagtc
//
LOCUS       DAOMUJ010000431         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20797_length_1638_cov_1.984207, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000431 DAOMUJ010000000
VERSION     DAOMUJ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20797_length_1638_cov_1.984207"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLT73_09080"
     CDS             complement(<1..500)
                     /locus_tag="PLT73_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006729164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRM20561.1"
                     /translation="MTITFSDFIDSIQTNPILLIIVVLTLGVILVNGWTDAPNAIATC
                     VSTRSIAPKKAILMAAVFNFLGVFVMTSINATVAQTIANMVDFGNDPKLSIIALCAAL
                     FAIVLWATAAWYFGIPTSESHALIAGISGAAIALQGGFGAINGSEWIKVIYGLFLSTI
                     LGFGFG"
     gene            857..>1638
                     /locus_tag="PLT73_09085"
     CDS             857..>1638
                     /locus_tag="PLT73_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HRM20562.1"
                     /translation="MVKRKLYIISLDAFGASDLEFAKTLPHFQEIFNRSARVKEVESV
                     YPSLTYVAHTSIATGMNPNRHGIIHNTHRQPERQSPDWYWYAKEIKKATMFDVAKQAG
                     YTICTLLWPVTGKSPSIDYNLAEIFPNRSWQNQVMVSAFASSTKYALEMNKKYGSLRN
                     GIAQPELDEFVTAIAVDTIKTKQPDLLAVHLVDLDSMRHEYGVLSDQAKEAVIRMDRH
                     LGQIIDAMKEMNIYEDTVLAVMGDHYQIDTHTVIRPNYLFLD"
BASE COUNT          482 a          344 c          393 g          419 t
ORIGIN      
        1 aatccgaagc cgaagcccaa gatggtggag aggaacagac cgtagatgac tttgatccac
       61 tctgatccgt tgatggcgcc gaacccacct tgaagtgcaa tcgcagcccc tgaaatgcct
      121 gcaatgagcg catggctttc gctggtcgga attccgaaat accaggctgc cgttgcccaa
      181 agcacgattg cgaaaagagc agcacagaga gcgataatcg aaagttttgg gtcgttcccg
      241 aaatcgacca tatttgcgat tgtttgggca actgtcgcgt tgatggatgt catcacgaac
      301 acgcccagaa agttgaatac ggcagccata agaatggctt tttttggagc gatggatcgg
      361 gtggatacac acgttgcgat tgcgttcgga gcatcggtcc aaccgttcac taaaatcaca
      421 cccaaagtca ataccactat gatcaaaaga atagggttcg tctggatgct gtcgataaaa
      481 tccgaaaatg taattgtcat agattgcact tccacgcttt cttcgtagcg ttctcaatta
      541 aatgaaacca tccgctttca tttcggaagg ctgcaaagta cagccggatt ccttgttggg
      601 cagaggcagc aagcttggtg agtccgctat cttattgtta gatgatgaac aatatacatt
      661 ctactccttc ctgaacaaaa ttagaatagc tttttttgaa agaaaagcag tgaacaatag
      721 actactgttt tcgtttttga aagacgcttc cgaaatgaat gctgtcacat tcgcaagaat
      781 actgaaatta aatcttttgc ggtatccatg cgggttctga tatatggtaa aatagtagga
      841 taggagggta tatattatgg taaaaagaaa actgtatatc atttcattag atgcatttgg
      901 tgccagtgac ttagaatttg ccaagacgct gccccatttc caggaaattt ttaacagaag
      961 cgcacgcgtc aaagaagtgg agtcggtcta tccatctttg acatacgtag cgcatacttc
     1021 aattgcaaca gggatgaatc caaaccgtca tggaatcatc cataataccc atcggcaacc
     1081 ggaaaggcag tcacccgact ggtattggta cgccaaagaa ataaagaagg caacgatgtt
     1141 tgatgtcgcc aagcaggcgg gatacacaat ctgcactttg ctttggccgg taacggggaa
     1201 aagcccttcc atcgattaca atctagctga aatttttccg aataggtctt ggcaaaacca
     1261 ggtgatggtt tctgcttttg cctccagcac aaaatatgcg ctggagatga ataaaaaata
     1321 tggttcattg cggaacggga tagcccagcc ggaattggat gaatttgtga ctgcgatagc
     1381 ggtcgataca atcaagacga agcagccgga tctgttggct gtccatctgg tggatctgga
     1441 cagcatgcgc catgagtatg gcgttttgag cgatcaagca aaagaagctg ttatcaggat
     1501 ggatcgccat ctcggacaga tcattgatgc gatgaaggaa atgaacattt atgaagatac
     1561 cgtgttggca gtgatgggcg atcattacca gatcgatacg cacacagtca ttcgcccaaa
     1621 ttacttattt ttggataa
//
LOCUS       DAOMUJ010000432         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20833_length_1636_cov_1.920936, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000432 DAOMUJ010000000
VERSION     DAOMUJ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20833_length_1636_cov_1.920936"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1052)
                     /locus_tag="PLT73_09090"
     CDS             complement(66..1052)
                     /locus_tag="PLT73_09090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HRM20563.1"
                     /translation="MVGIILASHGNFAEGILQSASMIFGEQENVKAVTLAPSEGPDDV
                     KAKMQEAISSFDNQDEVLFLVDLWGGTPFNQANSLFEAHKDTWAIIAGMNLPMVIEAY
                     AARLSMDSAQKIAAQILPAAKDGVRAKPESLEPKKVEAASAVQQPKGNLPVGTVVGDG
                     KIKLALVRLDSRLLHGQVATTWAKSAGTNRIIVVSDDVSRDELRKNLIVQAAPPGVKA
                     NVIPIKKMIEIAKDPRFGNTHALLLFENVQDVVRVIEGGVKIEELNVGSMAHSVGKVV
                     VNKVLSMGPEDVKAFEALKAKGVKFDVRKVPNDSSEKMDALLKKAKEELAKA"
     gene            complement(1261..>1636)
                     /locus_tag="PLT73_09095"
     CDS             complement(1261..>1636)
                     /locus_tag="PLT73_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM20564.1"
                     /translation="ESYDENELTSEKAKQICLEYMGESFTFLNPQKLIEPLWKFSSSL
                     LENKENYSQLINILMHLAGMFERALRQDTLIAPQEELKLTEQTERFQQLEQALNILSG
                     TFQIEMPKDEIYYLEQLLAYQE"
BASE COUNT          424 a          394 c          299 g          519 t
ORIGIN      
        1 aagaacgcca cgatgataac cagaataata gaaacgactg acataatata cctcctcttt
       61 tcttgttatg ccttagctaa ttcttcttta gcttttttca gcaacgcatc cattttttcc
      121 gatgaatcat tcggaacttt ccggacatca aatttaacac cttttgcttt caatgcttca
      181 aatgctttca catcttcagg ccccattgac aagaccttat tcacgacaac cttgccgact
      241 gagtgagcca ttgatccaac attcaactcc tcaatcttca cgccgccttc aattacccga
      301 acgacatctt gcacattttc aaaaagcaag agggcatggg tgttcccaaa acgaggatct
      361 ttcgcaatct caatcatttt tttgattgga atcacattgg ctttgactcc tggcggtgct
      421 gcttgcacga tcaggttttt ccgcaattca tctcttgaga catcatccga aaccacgatg
      481 atccgatttg ttccagctga tttcgcccac gttgttgcta cttgtccatg aagcaatcgt
      541 gaatccaatc gaactaaagc taacttgatt tttccatcac caacgactgt acctacagga
      601 aggttgcctt ttggttgttg cactgcagaa gccgcttcca ctttcttcgg ctctaaactt
      661 tctggcttag ctctcacacc gtccttcgct gctggaagaa tttgtgcagc aattttttgt
      721 gctgagtcca tgctcaatcg tgcagcatac gcttcgatga ccatcggaag gttcattccg
      781 gcgattatcg cccatgtgtc tttatgtgct tcgaaaagac tgttggcttg gttgaacggc
      841 gtgccgcccc ataaatcgac taagaacagt acttcatctt ggttgtcaaa ggatgaaata
      901 gcctcttgca tctttgcttt gacatcgtcc ggtccttcac taggcgccag tgtcactgct
      961 ttgacatttt cttgttcgcc gaaaatcatc gaagccgatt gcagaatacc ttcagcgaaa
     1021 ttcccatgac ttgctaggat aattcctacc atttgtatac ctcccattga atatattttg
     1081 cgactgtttg gataaaaagc ttggcaggcg atttaccagt cattatacta taagcaaaaa
     1141 gcgtgccaac ttagtgtatg gcttaatcat acggaattaa gaaacaaaaa aatacactaa
     1201 aacaattagt gttttagtgt atcgttcgca ctgaaagcta gtgtatggtt tactgtatca
     1261 ttattcttga taggccaaca gttgctccag atagtatatc tcatcttttg gcatttcgat
     1321 ttgaaaggtg ccgctcagaa tatttaaagc ctgttccaac tgctggaacc gctctgtctg
     1381 ttcagtcaat ttcagttctt cttgcggtgc aatcaatgta tcctggcgca aggcccgctc
     1441 aaacattccc gccaggtgca tcagaatatt gattaattgg gaataatttt ctttattttc
     1501 caataaggaa ctgctgaatt tccacaaagg ctcaatcagc ttttgtggat tcaagaaagt
     1561 aaagctttcg cccatgtatt ccagacatat ctgctttgcc ttttccgatg tcaactcatt
     1621 ttcatcatag gattcc
//
LOCUS       DAOMUJ010000433         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_20876_length_1634_cov_1.411020, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000433 DAOMUJ010000000
VERSION     DAOMUJ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20876_length_1634_cov_1.411020"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1398
                     /locus_tag="PLT73_09100"
     CDS             97..1398
                     /locus_tag="PLT73_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004131987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM20565.1"
                     /translation="MLVFSGTAAAATWLDVNGIIKDHSVEIIGQGSLDANTSIIDPNS
                     GKPIEFVLIGQDSRDGEENQSVGGNFDDVVGNHQADTTMVAQISADRTKINLVSIPRD
                     SLVDIPQCETTNGTIPAQYNVMFNSIFANAYQTGGDLASAASCTLNAVNSLTGLNIQN
                     FIVVDFAGLVKMINAVGGVDLCIPQDVDDPYTSLQLTKGLHHLDGHQATQYARTRHGL
                     GDGSDTSRTTRQQYLIKQLMNEALSKNLFTDTAQLYQLAKSALQSLYISQGMADTAAL
                     AGLAMSLKDFNLSNLYSQTVPVVSAPSDPNRSVWTDEAETLWEKMRADKPIYGSDESD
                     ANTDANTAGNSDGSSDNSTDGTDNTDNMGTDNTGSQEAAQTPDPVTGLITQADGTLID
                     PNTGGTVDPEDGSIHDALTGQYIGLADRYLNATVCAVPAKN"
BASE COUNT          412 a          502 c          413 g          307 t
ORIGIN      
        1 aacgtcaatg gactgcgaga cgcaaagccg cgtcacagcc ttggctatcg agtgcagcat
       61 cgtgcgagga cgatagtcgc ttcggtcatc gcggcgatgc tggtgttctc gggaaccgca
      121 gcggccgcaa catggctgga tgtgaatggc atcatcaaag accattcggt cgaaatcatc
      181 ggacaaggct cacttgacgc caacacctcg atcatcgatc ccaactccgg caaacccatc
      241 gaattcgtac tcatcgggca ggattcccgc gatggcgaag agaaccagtc cgtcggaggc
      301 aacttcgacg acgtggtagg caaccatcag gccgacacca caatggtggc gcagatttcc
      361 gcggaccgca ccaaaatcaa tctcgtttcc attccgcgcg actccctagt ggacataccg
      421 cagtgcgaaa cgaccaacgg cacgattccc gcgcaataca atgtgatgtt caactcgatt
      481 ttcgcgaacg cataccagac cggcggcgat ctcgcttcag ccgcaagctg caccctgaat
      541 gcagtgaaca gcctcaccgg actgaacatt caaaacttca tcgtcgtgga tttcgcagga
      601 ctggtcaaaa tgatcaatgc cgtaggcgga gtcgacctgt gcatcccaca ggacgtcgac
      661 gacccctaca ccagcctgca actcaccaag ggcctgcacc atctggacgg acaccaggca
      721 acgcagtacg cacgaacccg ccatggtctg ggagacggat ccgacacatc acgaacaacc
      781 agacagcaat acctcatcaa acagttgatg aacgaagcgc tcagcaagaa cctctttact
      841 gacaccgcac agctctacca gctagcgaaa tcagccttgc aatcactcta tatctcgcaa
      901 ggcatggccg ataccgcagc gcttgcaggt cttgccatga gcctcaagga tttcaatctg
      961 tccaacctgt attcgcagac cgttccggta gtatccgcgc cgtcggaccc gaatcgttcg
     1021 gtctggaccg atgaggcgga gacgctatgg gagaagatgc gcgcggacaa gccgatttat
     1081 ggttccgacg aatccgacgc gaacacggat gccaataccg caggcaattc cgacggttct
     1141 tccgataatt cgacggatgg cactgacaat acggataata tgggcactga caacacgggt
     1201 tcccaggaag ccgcacagac gcctgatccg gtcactggtc tgatcacgca agccgatggc
     1261 acgcttatcg atccgaacac cggtggcacc gtcgatccgg aagatggctc catccatgac
     1321 gctcttaccg ggcagtacat cggacttgcc gatcgttacc tcaacgcgac agtttgtgcc
     1381 gttcccgcaa agaactagac cgctttcacc gaatccgttc ctacacggtg taaatggtgt
     1441 aggaacggat ttctttttgg aggacattgt atgagtgaag agaacggcgg caaggacccc
     1501 atgcccgcac caccgagctt catcccgtct gcaggacgga agaagacgga atcacagcct
     1561 cacaaatcca caattccttc tcaatccggc tctccgtcaa ttccgagttt ttcgcctgcc
     1621 gcacagccag cgcg
//
LOCUS       DAOMUJ010000434         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21060_length_1621_cov_2.714559, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000434 DAOMUJ010000000
VERSION     DAOMUJ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21060_length_1621_cov_2.714559"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLT73_09105"
     CDS             complement(<1..374)
                     /locus_tag="PLT73_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20566.1"
                     /translation="MDLQVSPITLMLSFSLVMVGVYISYKEKLGTAKDILYSIARAIV
                     QLIAVGYVLTYLFNVNNTIVTLAMVAVIVFNASWNAHKRSSSIPNSLKISLIAISASA
                     ILTLAILVLSGSVKFIPSQIVP"
     gene            complement(376..1029)
                     /locus_tag="PLT73_09110"
     CDS             complement(376..1029)
                     /locus_tag="PLT73_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20567.1"
                     /translation="MALLEMEHLSFVSDGKVILDDISYSLEEGEFLSITGPSGSGKST
                     LLKIIATILSRTEGEIRYQGKSLDEYEPTEYRKEVSYTFQTPVLFGKTVRDNLAFPYE
                     IRKKEFDEKKAVAYLESVGLPKEYLNKEINTLSGGEKQRVALIRNVLFQPKILLLDEV
                     TSALDEANRQIIWKWLQEMRNHSDMTIIMVSHNEEESSLADKNIHIVAGRIVKEEAK"
     gene            complement(1435..>1621)
                     /locus_tag="PLT73_09115"
     CDS             complement(1435..>1621)
                     /locus_tag="PLT73_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HRM20568.1"
                     /translation="KPDTSVELIVHPGYVDASLERVSSLQKDRAYMAEFLMHSDFADR
                     IREDEAIQLISYAELGE"
BASE COUNT          421 a          399 c          346 g          455 t
ORIGIN      
        1 atcggcacaa tctgtgaagg gataaatttg acggacccgg acaggaccaa aatggctaac
       61 gtcaaaatcg ctgaagcaga gatggcgatg agcgaaatct tcaaagaatt cgggatactg
      121 ctgctgcgtt tgtgggcatt ccatgatgca ttgaagacga tgaccgcgac catcgccaaa
      181 gtgacgattg tgttattcac attgaacaga taggtcaata cgtacccgac cgcgatcaac
      241 tgcacaatgg ctctggcaat gctgtacaag atatctttgg ccgttcctaa tttttccttg
      301 tacgagatgt agactcccac cattaccaac gaaaagctca acatgagcgt gattggactg
      361 acttgtagat ccatattatt ttgcctcctc ttttacgatt cttccagcaa caatatggat
      421 attcttatcc gccaagctgg actcttcctc gttatgactg accataatga tcgtcatatc
      481 ggaatgattc cgcatttcct ggagccattt ccagatgatc tggcgattcg cttcatccaa
      541 ggcgctcgtc acttcatcga gcaagaggat ttttggttgg aaaaggacat tgcgtatcaa
      601 ggcgacacgc tgtttttctc cacccgacaa ggtgttgatc tctttattca gatattcctt
      661 cggcagcccg accgattcca aatatgcgac tgctttcttt tcatcgaatt cctttttgcg
      721 gatttcgtaa ggaaaagcca aattgtcgcg gacggttttt ccgaacaaaa caggtgtttg
      781 gaatgtgtag gaaacctcct tgcggtattc ggtaggttcg tattcatcca gcgacttccc
      841 ttggtagcgt atttcccctt cggtacgcga aaggatcgtc gcaatgatct tcaaaagtgt
      901 gctcttgccg cttccggacg ggccggttat cgagagaaac tcgccctctt ccagcgaata
      961 gctgatgtca tccagaatga ctttgccgtc cgacacaaaa ctcagatgtt ccatttccaa
     1021 taatgccatt ttttagattg ctcctttatc attcaaattg catccaaacc cgatgcaaac
     1081 tccaaaaacc gctgttcagg ttggtaatgc ttcattcttc tattctatca ctgattccct
     1141 attttttgtt aatgataatg attataattt agcaacaaat acaaaatgat actattggac
     1201 taaaatatgc tgatcagagg agcaacccag tttactcagt ttcttcctgt agctccggtg
     1261 aggcagatgc tcatcggagg atagcggtaa ctatccgcct caaattcggt gaggccggtc
     1321 tcatcggagc acacaagccc gaatgcagtc tgaactccgg tcaagccttc attcgccgga
     1381 gaacggcaaa ggctgtctcg aaaaacgaga cagcctttgt tatttcttat cgtattactc
     1441 tcccaattcc gcatacgaaa tcaactggat tgcctcatct tcacggatgc gatccgcaaa
     1501 atctgaatgc atcaagaatt cggccatata ggctcgatcc ttctgcaggc tactgacgcg
     1561 ttccagactg gcatcgacat aacccggatg gacgatcaat tcgaccgagg tgtccggctt
     1621 t
//
LOCUS       DAOMUJ010000435         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21120_length_1618_cov_1.628919, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000435 DAOMUJ010000000
VERSION     DAOMUJ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21120_length_1618_cov_1.628919"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1618
                     /locus_tag="PLT73_09120"
     CDS             61..>1618
                     /locus_tag="PLT73_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HRM20569.1"
                     /translation="MDTPEELEEFFDKFSLETKLKSYCFPLGTPISISAGKTKPIEDI
                     KPGDTVLCFDPIADFGRGTLVPKRVVRLFHNETEEWLRLTWREDGEDHELTVTPGHRF
                     VDAAGRFWRIDEIVKDAAPTVVLADGSLMQVRAEQIVWSQETRHLFEEADAVAMAAGD
                     GLAHRTGGAWRCYNFEVQDLHTYVAGGVRVHNDSQATIDLAGNLGRAFGSQLTNVLLE
                     GENQFTKVLGGTVLSTITENLAEVITSTGYHMFDPHKFSFGQSFEDAVGNQLGDIGTE
                     FMANLSSTMTSLIIAEFGEKLGLEGFGAELFSVTGSAYAGSVLEQLTKNKYDWGEVDW
                     DAAWSSVPGAIGTFFGSSLAHKILPAETLEGSIGGSLGSIVGTSWAIGQIGGGAAGMQ
                     SLGLLGNLLIPGVGAFIGTLLGTLVGDLFSDKPDPGADFLMFAEQQGENIIPGMLDYY
                     LYATAQDGFPHDYTKALGEAVIELARGYMSNIGAFDMSNANVTGFRLPSQYSGKDPHN
                     LGANPLVRVLQ"
BASE COUNT          330 a          443 c          500 g          345 t
ORIGIN      
        1 catggacaac tggatcaagg cctccggcga ggtgctgcac gacgagcgct acttgaaaac
       61 ttggatacgc ctgaggagct tgaagagttt ttcgacaaat tttcgttgga aaccaagctg
      121 aaatcatact gcttcccgct cggcactccg atttcaattt ccgcgggcaa gaccaagccc
      181 atcgaagaca tcaagccggg cgataccgtt ttgtgcttcg atcccatagc tgatttcggt
      241 cgcggcacgt tggtgcccaa gcgtgttgta cggctgttcc acaatgaaac agaagaatgg
      301 ctccggctga catggcggga ggacggtgag gatcatgaac tgacggtgac gccagggcac
      361 cggtttgtgg acgccgcggg gcggttctgg cggattgacg agatcgtcaa ggacgccgcg
      421 ccgactgtcg ttttggccga tggctcgctt atgcaggtcc gggccgagca gatcgtctgg
      481 tcgcaggaaa cccggcatct gtttgaggag gccgatgcgg tcgccatggc tgcgggtgac
      541 gggcttgcac atcgcaccgg cggcgcgtgg cgctgttaca acttcgaggt ccaggatctg
      601 cacacctatg tggcgggcgg tgtgcgggtg cacaatgaca gtcaggccac gattgatctc
      661 gcggggaatc ttggtcgcgc ctttggctcc caacttacca acgtgcttct ggaaggcgag
      721 aaccagttca ccaaagtgct gggcggcacg gttctgagca cgatcaccga aaatctggcc
      781 gaggtgatca ccagcaccgg ctatcacatg ttcgacccgc ataaattcag cttcggacaa
      841 tccttcgagg acgccgtcgg gaatcagctt ggcgacatcg gcaccgagtt catggcgaac
      901 ctctcatcga cgatgacgtc tctgattatc gccgaatttg gcgagaagct cggtctggag
      961 ggcttcggag cggaactttt cagcgtcaca ggctcggcct atgccgggtc cgttctcgaa
     1021 cagcttacta aaaacaaata cgattggggt gaagtagact gggatgcggc gtggtcaagt
     1081 gtcccaggag caatcgggac attctttggc tcatctctcg cgcataagat cctgcctgcc
     1141 gaaacgctgg agggctcaat cggtggatcc ctcggctcga tcgtcggcac ctcctgggca
     1201 ataggtcaga ttggcggcgg cgctgcgggg atgcaatcgc tgggattgtt gggcaacctt
     1261 ctgatccccg gggtgggtgc gttcatcggc acgcttctgg gcactttggt tggtgatctg
     1321 ttcagcgaca agccggaccc cggcgccgac ttcctgatgt ttgccgagca gcagggcgaa
     1381 aacatcattc ccggcatgct cgactactat ctgtacgcga cggctcagga cggctttccg
     1441 catgattata ccaaggctct tggcgaggca gtcatagaac tcgcgagggg ctatatgagc
     1501 aatatcgggg cgttcgacat gtcgaacgcc aatgtgaccg gcttcaggct gccctcgcaa
     1561 tatagcggta aggacccgca caatctgggt gccaatcccc tggtccgcgt gctgcaaa
//
LOCUS       DAOMUJ010000436         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21138_length_1616_cov_2.137092, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000436 DAOMUJ010000000
VERSION     DAOMUJ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21138_length_1616_cov_2.137092"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLT73_09125"
     CDS             complement(<1..904)
                     /locus_tag="PLT73_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HRM20570.1"
                     /translation="MPASNEEIREGLSILFENFWIIRETDKDVYHQLLRIENDLKRVT
                     QRIFGLRLIVHAKFIKLEKIPFESQDWMGITTFNEPMDYALFACAMAYTEDKSEGEAF
                     LINELVDELDLFYPERDLLDWTNFNHRKSLVRVLNVMTDMQLIDTIEGKTDDFATSES
                     NEVLYKVSPYSKYFMRSYPEDITQFQDWRDLVQMERERADEETSTVAFRRLMMEPAIL
                     RTEENDRLFYYLRNQQQYISAFFEKNTPYTFELTKDVAMLTLEERLKQQTYFPMLKSA
                     DESLLQLAEMIRESDYAPDAYGCIH"
     gene            complement(888..>1616)
                     /locus_tag="PLT73_09130"
     CDS             complement(888..>1616)
                     /locus_tag="PLT73_09130"
                     /inference="COORDINATES: protein motif:HMM:NF021197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2397 family protein"
                     /protein_id="HRM20571.1"
                     /translation="IWDTMLAWFIDGQGKSSEYSSLMKQTNQAISKITRMIQRFSDRM
                     QQHQSRKKDYIHLAEWFLHCRSVEEAHQLSSQVFGAFHTQHYYVKPFETSNKFADIWD
                     IPATVHETIPHIRAYRERTSATAFSRNTQAKKEAVAAHLQQRQELEKSLQHYIQNGKI
                     DLIEHPFMDASIRKLLLKWLSLSFQNEERTIATEFGQVFAIKVDRSIKVRLESEDGFL
                     EMPNVVYVLIDKENTGGDRNAGKQ"
BASE COUNT          355 a          425 c          389 g          447 t
ORIGIN      
        1 aatggatgca cccataagcg tcaggggcgt aatccgattc ccggatcatt tccgccagct
       61 gcagcaggct ctcgtcggcg gatttcagca tcgggaaata ggtctgttgc ttcaggcgtt
      121 cctcaagcgt cagcatcgcc acatctttcg tcaactcgaa ggtgtacggc gtattttttt
      181 caaaaaaagc actgatgtac tgttgctgat tacgcaggta atagaacagc ctgtcgttct
      241 cttcggttcg gaggatcgcc ggttccatca tcagccgcct gaaagcgacc gtgctcgtct
      301 cctcgtcggc ccgctcccgt tccatctgca ccaagtcccg ccaatcctgg aattgggtga
      361 tatcttccgg ataggaacgc atgaaatatt tgctgtaggg gctgactttg tagaggactt
      421 cattggactc ggaggtcgcg aaatcatcgg tcttcccctc gatcgtatcg atcaattgca
      481 tgtcagtcat cacgttcagg acgcgcacta ggctttttcg gtggttgaaa ttggtccagt
      541 ccaggaggtc ccgttcagga taaaacaggt ccagttcatc aacgagttca ttgatcagaa
      601 acgcctcgcc ttcgctttta tcctccgtat aggccatcgc gcaggcaaac agggcataat
      661 ccatcggctc gttgaaagtc gtgatgccca tccaatcctg ggattcaaat gggatttttt
      721 ccaacttgat gaatttagca tgcacaatca agcgcagccc gaatattctc tgggtgacgc
      781 gcttcaggtc gttctcgatg cgcaaaagct gatggtagac gtccttatcg gtttcccgta
      841 tgatccaaaa attttcaaaa aggatactga gtccttctcg gatttcctca ttgcttgccg
      901 gcattcctgt cacctcctgt attttccttg tcaatcagca catagacgac attcggcatc
      961 tccaaaaagc cgtcttcgga ttccaacctg acttttatgc tcctgtccac tttgatcgca
     1021 aatacttggc cgaattcggt tgcgatggtc cgctcttcat tctggaaaga caggctcagc
     1081 catttcaaca acaatttccg gatcgatgca tccataaagg gatgttcaat caaatcgatc
     1141 tttccgttct ggatataatg ctgcagggat ttttcgagtt cttgccgttg ctgcagatgg
     1201 gccgctacgg cttccttctt cgcctgggta tttcgcgaga aggccgtcgc gcttgtccgt
     1261 tcccgatacg ctctgatatg cggaatcgtc tcatggacgg tcgccggaat atcccagatg
     1321 tccgcaaatt tgttgctggt ttcgaagggc ttcacataat agtgctgcgt gtgaaaggca
     1381 ccgaatacct gcgaggaaag ctgatgcgct tcctcaacgg accgacagtg caggaaccac
     1441 tcagccaagt ggatatagtc ctttttccgg ctctgatgct gttgcatgcg gtcgctgaaa
     1501 cgttggatca tccgggtgat tttgctgatg gcctgattgg tctgtttcat taaggaactg
     1561 tactcactgg acttcccttg cccatcgatg aaccaagcaa gcatcgtgtc ccaaat
//
LOCUS       DAOMUJ010000437         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21254_length_1609_cov_1.904762, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000437 DAOMUJ010000000
VERSION     DAOMUJ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21254_length_1609_cov_1.904762"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLT73_09135"
     CDS             complement(<1..182)
                     /locus_tag="PLT73_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HRM20572.1"
                     /translation="MNVPVFLEFVEIKTKLASLFPFVLGTLFTLYHFGSINALNTILF
                     FIAMVVFDMATTAINNT"
     gene            complement(194..1261)
                     /locus_tag="PLT73_09140"
     CDS             complement(194..1261)
                     /locus_tag="PLT73_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM20573.1"
                     /translation="MRKYMNKLVLTGALLLSLLIGGCGNGGVQEETAIRKEPYEKTEF
                     LMGTYVTVRVYDEGKEAVLEEAFAHVEELADKITVNEPGSEIDAINAVAGTEAVELSE
                     DIYPLVRSAWDYSKASDGNFDLSIGPITELWHIGFEDARKPEQSEIDAALALVDYERV
                     VLDDAARTVQLADAAMRLDLGAIAKGYITDEVKDLLAENGVTTAIIDLGGNVYVMGGS
                     PLREGESWNVGIQDPMAARGETIGKTKQKNRSIVTSGIYERYIEVEGVSYHHLMDPET
                     GYPFDNDIAGVSILSDKSIDGDALSTLVFGLGREAGLAYVNERDDIEAVFVTKDKKVY
                     VSDGLIDNFELTNDDYVWENE"
     gene            complement(1377..>1609)
                     /locus_tag="PLT73_09145"
     CDS             complement(1377..>1609)
                     /locus_tag="PLT73_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM20574.1"
                     /translation="DAEYQANMAEKVGVGPADYFPQLNNQLIETQDPEAVEVVTGATS
                     STDTFKKYAPMLIEAAEAGDTTTIEIDNAVEE"
BASE COUNT          411 a          466 c          310 g          422 t
ORIGIN      
        1 gtgttattga tggccgtcgt cgccatatca aatacaacca tcgctataaa gaataaaata
       61 gtgttcagcg cattgatgct gccgaaatga taaagcgtaa acaaggtacc cagcacaaac
      121 ggaaacaaac tggctaactt agtcttgatt tccacaaatt ccaaaaatac aggtacattc
      181 atctctttca cccctattca ttttcccaaa cataatcgtc gttcgtcagt tcaaaattat
      241 caatcaatcc atccgaaacg tagactttct tgtccttagt cacaaaaacg gcttcgatat
      301 catcccgttc gttgacatag gccagaccag cttccctccc gagaccaaag acaagcgtcg
      361 acaaggcatc gccatcgatc gatttatcgg acaaaataga aacaccggcg atatcgttat
      421 cgaacggata gcccgtttcc ggatccatca gatggtgata gctgacaccc tccacttcga
      481 tatagcgttc gtaaatgcct gaagtcacga tcgaacggtt tttttgtttc gtcttgccga
      541 tcgtttcccc acgcgcagcc atcggatcct ggatgcctac attccaagac tccccttcac
      601 gcaaaggcga accgcccatg acataaacgt tgccgccaag atcgatgatg gccgtcgtaa
      661 caccattctc cgccaacaaa tccttcactt cgtcggtgat atagcctttt gcgatggcac
      721 ccaagtccaa gcgcatcgcc gcatcagcca actgaaccgt ccgcgctgca tcatccagca
      781 cgacgcgttc gtaatcgacc aacgccagcg ccgcatcgat ctcactctgc tccggcttcc
      841 tagcatcctc gaagccgatg tgccacagtt ccgtgattgg cccaatcgac aggtcgaaat
      901 taccatcgga cgccttgctg taatcccaag cactccgcac caacgggtag atatcctcag
      961 atagttcaac cgcttccgtc ccggcaacag cgttgatcgc atcgatctca gatccaggtt
     1021 cattcactgt aatcttgtcg gccagttcct cgacatgagc gaaagcttct tccagcaccg
     1081 cctctttgcc ttcatcataa acgcgcaccg tcacataggt gcccatcaaa aattccgttt
     1141 tctcgtacgg ctctttgcgt atcgccgttt cttcctgcac gccgccgttg ccgcatcccc
     1201 cgataaggag cgacagcaac aaggcgccgg tcaagacgag cttattcata tattttctca
     1261 tcgattcacc tcatattccc atttaaaaaa gacaggcagg aacaagaacg ctgtccctac
     1321 ctgtctataa taatcttttt tcttgctgaa tccacctaaa aaagtggaaa aacgccttat
     1381 tcttcgactg cgttgtcgat ttcgattgta gttgtgtcgc cagcttcagc tgcttcaatc
     1441 aacattggtg catatttttt gaaagtatca gtagagcttg ttgctccagt aacgacttcc
     1501 accgcttccg gatcttgcgt ttcgatcaat tgattgttca attgagggaa gtaatcagca
     1561 ggacctacgc caactttttc tgccatgttt gcttggtatt ctgcatctt
//
LOCUS       DAOMUJ010000438         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21395_length_1601_cov_2.023933, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000438 DAOMUJ010000000
VERSION     DAOMUJ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21395_length_1601_cov_2.023933"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_feature    65..138
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            155..463
                     /gene="rplU"
                     /locus_tag="PLT73_09150"
     CDS             155..463
                     /gene="rplU"
                     /locus_tag="PLT73_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRM20575.1"
                     /translation="MYAIIKTGGKQLKVEVGQAIYIEKLDAEAGDKVTFDEIVFVGGE
                     ETKIGAPFVTGASVEGTVEKQGKEKKVTTFKYKRRKDTHRKQGHRQPYTKVIIDAINA
                     "
     gene            478..813
                     /locus_tag="PLT73_09155"
     CDS             478..813
                     /locus_tag="PLT73_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRM20576.1"
                     /translation="MIEVKFKEDDHGNLLSFEITGHAGYGVEGEDIICAAVSVLAIET
                     VNSVERLAGYQMLVDEADEEGGYLYAEILSEREAEQQYITQILLKHLFYSLEDVAQTY
                     PDYVTINMQ"
     gene            848..1132
                     /gene="rpmA"
                     /locus_tag="PLT73_09160"
     CDS             848..1132
                     /gene="rpmA"
                     /locus_tag="PLT73_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM20577.1"
                     /translation="MLKLNLQLFAHKKGGGSTTNGRESQSKRLGAKRADGQTVTGGSI
                     LYRQRGTKIHPGTNVGIGGDDTLFAKVDGVVRFERLGRDKKQVSVYPTAK"
     gene            1263..>1601
                     /locus_tag="PLT73_09165"
     CDS             1263..>1601
                     /locus_tag="PLT73_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM20578.1"
                     /translation="MKATNVESLFGLMDEAITLLQTEAELTYIEALSETLENLSFEAK
                     AQQVEGLPSIEAVERLNSLYKKMNLDALDKEIIRKSIQLTFIKATKEDKLQMNHQMTP
                     DTIAYLIAYFI"
BASE COUNT          538 a          302 c          360 g          401 t
ORIGIN      
        1 gttctgattt gggttgactt gagtcttgat ttttgataaa ctattaatgt tgtaaatgtg
       61 cgcaccacag ctacaaccgc acggatagga gtcatgagac ctgcaagggc actccgtttg
      121 gcgagtctaa gtaaaataag gaggtgcaga gaatatgtac gcaattatca aaactggtgg
      181 taaacaatta aaagttgaag ttggtcaagc aatctacatt gaaaaattag atgctgaagc
      241 tggtgataaa gtaacttttg atgaaatcgt atttgtaggc ggagaagaaa ctaaaattgg
      301 cgctccattc gtaacaggtg cttctgtaga aggcactgtt gaaaaacagg gcaaagaaaa
      361 gaaagtcact actttcaaat acaagagaag aaaagataca caccgtaagc aaggtcatcg
      421 tcaaccttat acaaaagtaa tcattgacgc aatcaacgca taagagtgtg aataaccatg
      481 attgaagtga aatttaaaga agatgaccat ggcaacctgc tttctttcga gattacggga
      541 cacgccgggt atggggtaga aggcgaagat atcatctgcg ctgctgtttc tgtactggcc
      601 atcgaaactg tgaatagcgt tgaacgttta gccggctatc agatgcttgt tgatgaagcg
      661 gatgaagaag gcgggtatct ctatgcggaa atactttccg aaagggaagc ggaacagcaa
      721 tacattacgc agattttact gaagcatcta ttctactcgt tggaggatgt cgctcagaca
      781 tatccagatt atgtgaccat aaacatgcaa taaatactgt actacttaat aaggaggtgc
      841 aaaccacatg ttgaaattga atctacaatt gttcgcccac aaaaaagggg gcggatctac
      901 taccaacgga cgtgagtccc aatccaaacg tttaggcgct aaacgtgcag acggacaaac
      961 agtaactggc ggatcaattt tgtaccgtca acgcggaaca aaaatccatc caggtactaa
     1021 cgtcggtatc ggtggagatg acactctatt tgcaaaagtt gacggtgtcg ttcgtttcga
     1081 acgtttaggc cgtgacaaaa aacaagtttc tgtataccct acagctaaat aatacttgta
     1141 cttttgccga tcaaccaatt acatacacac aaaaaacctt gcgggggcaa atgatcaatc
     1201 acttccttcg caggtttttt tgtattttag ataaagacaa aaagtaaggg acggtgaacg
     1261 acttgaaagc aacaaatgtt gagagtcttt tcggattaat ggatgaagcc atcacattgc
     1321 ttcaaacgga agcggagctt acatatatag aagcgttatc cgaaacgttg gaaaatcttt
     1381 cctttgaagc aaaagcacaa caagtggaag gcttgccttc aatcgaggca gttgaacgct
     1441 tgaacagcct ctacaaaaag atgaatttgg atgcattgga taaggaaatt atccgcaaga
     1501 gcatccaact gactttcatc aaggcaacaa aagaagacaa gctccagatg aatcatcaaa
     1561 tgactccgga cacgattgcg tatctgatcg catattttat c
//
LOCUS       DAOMUJ010000439         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21398_length_1601_cov_1.900388, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000439 DAOMUJ010000000
VERSION     DAOMUJ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21398_length_1601_cov_1.900388"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLT73_09170"
     CDS             <1..886
                     /locus_tag="PLT73_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM20579.1"
                     /translation="ELVELYSADDAVYGIPKDYDTIGLWYNKTLFDAAGVAYPDETWT
                     WDTMLEAAQKLNDPDNGVYGILAPLNRQEGYHNFIFQNGGYILSDDKTESGFRLDETI
                     EAVQWYADLSVKYGVSPTQSQFAENSNMSFFQSGRGAMGFFGSWTTGEMANNDYTAAN
                     ADVAPLPKGKQAATVFNGLANSVAASTENEEAALKFVEFLGTEEAMRLQGENGAAIPA
                     YEGTDESFVNAYSQFNIQVYVDQMENAYIKPYSKETARWEDVENNALMPVFDGTKSVA
                     DVAEEIVAGVEEVLANEK"
     gene            951..>1601
                     /locus_tag="PLT73_09175"
     CDS             951..>1601
                     /locus_tag="PLT73_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM20580.1"
                     /translation="MMKSDVTIPKEKTLSTVTPTQRKRKMTLWGWFFIAPTLIGLLIL
                     NVIPLFQSLYMSFQSVSTFGTSTFVGLDNYLKMLQDPEFWQSLKNTFFYAGIQVPITI
                     ILSLFFAVLMNTKIKAVGLYRTIFFLPMIAAPAAVAMVWRWLFNSEYGLLNAVLDKLG
                     YSGNILWITDPEVAIWSIIIVGIWTNVGYNMILLLAGLKEIPKEYYEAAVVDGAGPL"
BASE COUNT          453 a          350 c          399 g          399 t
ORIGIN      
        1 agaattggtt gaattgtatt ctgctgacga tgcagtctac ggcatcccga aagattacga
       61 tacgattggg ttgtggtaca acaaaacgtt gtttgatgcg gcaggcgttg cctatccgga
      121 tgaaacctgg acttgggaca caatgctgga agccgctcaa aagctgaacg atcccgacaa
      181 cggcgtatac ggtatcctgg ctcctttgaa cagacaagaa ggctaccaca acttcatctt
      241 ccaaaatggt ggctacatat tgtccgatga caagactgaa tccggcttcc gcttggatga
      301 aacgatcgaa gcagtgcaat ggtatgcgga tctaagcgtg aaatatggcg tgtcgccgac
      361 acaaagccaa tttgctgaaa attcaaacat gtccttcttc caatcaggac gcggcgcaat
      421 gggcttcttc ggatcttgga cgacaggcga aatggcaaac aacgactata cggctgcaaa
      481 tgctgatgtt gcaccacttc caaaaggcaa acaagcggca acagtgttca acggcttggc
      541 gaactccgtt gcggcttcaa ccgaaaatga agaagcggct ctgaaatttg tggaattctt
      601 gggaacagaa gaagcgatgc gtctgcaagg cgagaacggc gcagcaattc ctgcttatga
      661 aggaacagat gaatcattcg taaatgctta cagtcaattc aatatccagg tctatgtgga
      721 tcaaatggaa aatgcgtaca tcaagcctta ctccaaagag acagcacgtt gggaagatgt
      781 tgaaaacaac gccttgatgc cagtattcga cggtacgaaa tccgttgcag atgttgctga
      841 agaaattgta gctggtgttg aagaagtttt agcgaacgaa aaataacaaa atagaaggcc
      901 acttacggga ataatcccat aagtggcttt caattctata gaggtgaaat atgatgaaat
      961 cggatgtaac aatccctaaa gaaaaaactt taagtacggt gacacctacg caacgcaaac
     1021 gaaaaatgac actgtggggc tggtttttca tcgccccaac cttgatcgga cttttgatcc
     1081 tgaacgtcat cccgttgttc caatcgctgt acatgagttt ccagagcgtg agcacgttcg
     1141 gcacgtcgac cttcgtcggg ctggacaatt atctgaaaat gctgcaagat cctgaatttt
     1201 ggcaatcttt gaagaatact tttttctacg ccgggatcca agtacctatc accatcatcc
     1261 tgtcgctgtt ctttgcggta ttgatgaata caaagataaa agcggtcgga ttgtaccgga
     1321 cgatcttctt cttgccgatg attgcagctc ctgctgccgt agccatggtt tggcgctggc
     1381 tgttcaactc ggaatacggc ttgctgaatg cagtgcttga taaactcggt tattccggaa
     1441 atatcttatg gatcaccgat ccggaagtgg cgatctggtc gatcatcatc gtcggcatct
     1501 ggacgaatgt cggctacaac atgatcttgt tgttggcagg gctgaaggaa atacctaaag
     1561 aatattatga ggcggctgtc gttgacggtg ccggaccgtt g
//
LOCUS       DAOMUJ010000440         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21439_length_1599_cov_1.576425, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000440 DAOMUJ010000000
VERSION     DAOMUJ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21439_length_1599_cov_1.576425"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLT73_09180"
     CDS             <1..808
                     /locus_tag="PLT73_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM20581.1"
                     /translation="SEFVATLENEIELVQDEIRGIAFSCPGKVDTDTGMISFGGALPF
                     LDGVSFVEIFQPKFQVPISVINDGKAAALSELWLGNLNGIENGLALVLGTGIGGGLIL
                     EGKLYQGKNFQAGELSFMMKPSDKPSFDDTYGKTGSAVGLVKSVNRVLGTEDLKDGAA
                     AFEAINQKDPNVYPIFEAFAREIAYMICNVQAILDLEKIVIGGGISAQPIVTEEIRKQ
                     YRAVRAELPFVESTLTEVEIDSCRFMNDANLLGALYQLLLNADEEMAVNV"
     gene            1051..1500
                     /locus_tag="PLT73_09185"
     CDS             1051..1500
                     /locus_tag="PLT73_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM20582.1"
                     /translation="MNSKEVALMFDLPIDTIRYYERAGVIPPITRDKNGYRIYAKRDL
                     NWIFLAKSLRKAGLSIESLIEFATLAQSKEGNVRAAQKQILQDQLTEINAKLEEMMHA
                     RDLLEYKIETFDDHVAKINAGELSDDEVEELWTMKHFKKQEIPLAVE"
BASE COUNT          457 a          340 c          439 g          363 t
ORIGIN      
        1 cagtgaattc gtcgcgacat tggaaaacga aattgaattg gtgcaggatg agattcgcgg
       61 aatcgccttc agttgccccg gtaaagtcga tacggatacg ggcatgattt ccttcggcgg
      121 agcgttgcct ttcctcgatg gcgtttcctt cgtcgaaata ttccaaccga aatttcaagt
      181 tcccatctcg gtcatcaatg acgggaaagc ggctgcgttg tcagaattgt ggctcggtaa
      241 tctgaatggc atcgaaaacg gactggcttt agtgctcgga accggcatcg gaggagggct
      301 gatacttgaa ggcaagctct atcaaggcaa gaatttccaa gccggcgaat tgagcttcat
      361 gatgaagccg tctgataagc ctagtttcga tgatacgtac ggtaagacgg gctcggcggt
      421 cgggctagtt aaaagcgtga atcgggtgtt ggggacagag gatctgaagg acggggcagc
      481 tgccttcgaa gcgatcaacc aaaaagaccc gaacgtctat ccgattttcg aagccttcgc
      541 cagggaaatc gcctatatga tctgcaacgt ccaagccatc cttgacctcg agaagatcgt
      601 catcggcggc gggatcagcg cccaaccgat cgttaccgaa gaaatccgca aacaataccg
      661 cgccgtccgt gcagagctgc ctttcgtgga aagcacattg acggaagtcg aaatcgacag
      721 ctgccgcttc atgaatgatg cgaatctgtt gggggcgctg taccagttgt tgctgaatgc
      781 ggatgaggag atggcagtga acgtctgaaa aacacgcatc gtaagcaggt aatgtattca
      841 ttcatcctgc aatcaaatcc aaaaatacaa actgctcatg atccgtgcag agacgcaggg
      901 gtcatgagcg gtttatgtgt gtttgaggta gagggcatcg gacaccaaag aaatcaaaat
      961 ccagcttcga accgcacctg atttgacctg aagtgcactc catggtttat aatggatgca
     1021 tagacaaagt acaagagaga agtgaagatg atgaacagta aagaagtagc gctgatgttc
     1081 gacctgccga tcgatacgat cagatattat gaacgggcgg gtgtgattcc gccgataacg
     1141 cgcgacaaaa atggctaccg catctatgcc aaacgggacc tcaattggat ttttctggcg
     1201 aagagtttgc gcaaggcggg cttgtcgatc gagtcgctga tcgagttcgc aaccttggcg
     1261 caatcgaaag agggtaatgt tcgggcagcc caaaaacaga ttctgcagga tcagttgaca
     1321 gagatcaatg ccaagctgga ggagatgatg cacgcaaggg atctgctgga gtacaagatc
     1381 gagacgtttg atgatcatgt cgctaaaatc aatgccggtg aactgagtga tgacgaggtt
     1441 gaagagctct ggacaatgaa acatttcaaa aaacaggaaa taccgcttgc tgtggagtaa
     1501 actccatagt atagagtaaa cattgtagta aatattccaa ttggaggtta tgatatgcaa
     1561 acagtaaaat taaacaacgg tgttgagatt ccagttttg
//
LOCUS       DAOMUJ010000441         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21449_length_1598_cov_2.134154, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000441 DAOMUJ010000000
VERSION     DAOMUJ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21449_length_1598_cov_2.134154"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLT73_09190"
     CDS             complement(<1..551)
                     /locus_tag="PLT73_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20583.1"
                     /translation="MENILEVKDLQITFDTYAGKVKAIRGVSFDLKPGETLAIVGESG
                     SGKSVTSRSIMRLLANNANIEHGEILFKGEDLVHKTEKEMQKIRGSEIAMIFQDPMTS
                     LNPTQKIGKQIAEPIIKHQNISKDEAYKKAEELLQLVGIPNPAKRMKQYPHQFSGGQR
                     QRIVIAIALGCNPDILIADEPTTA"
     gene            complement(563..>974)
                     /locus_tag="PLT73_09195"
     CDS             complement(563..>974)
                     /locus_tag="PLT73_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20584.1"
                     /translation="IIALALTEWLSMARVVRAQTLKLKNQEYILAARTLGQSPMKIAF
                     RHILPNLAGVIIIQVMFSIPSAIFFEAFLSFIGIGIPAPNASLGTLINDGYKTFRFLP
                     HLMWYPAGIMSVIMICFNLLADGLRDAFDPKMRD"
     assembly_gap    975..984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<985..1567)
                     /locus_tag="PLT73_09200"
     CDS             complement(<985..1567)
                     /locus_tag="PLT73_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20585.1"
                     /translation="MPTITKDLFESASESSMQDKEKISAPSLSFMADSWRRLKKNKVA
                     MVSLIILIIISMSSLLAPVIAPHDPNAQTVQYANMPPKIPGIDINGLNGTVKQNGVVI
                     DKYEASNVPEDVYYYLGTDSLGRDLLSRILYGTRVSLFIAFMAALFNLTLGVVYGLTS
                     GWLGGKVDNIMQRILEILSGVPNLVVVILMLLVL"
BASE COUNT          420 a          364 c          364 g          440 t
ORIGIN      
        1 gctgtagtcg gttcatccgc aatcaaaata tctgggttgc agcccaaggc gattgcgata
       61 acgatccgtt gacgttgtcc acccgaaaat tggtggggat actgtttcat tcttttggca
      121 ggattcggga tgcctacaag ctgcaacaat tcttcagctt ttttataagc ttcatctttg
      181 ctgatgtttt ggtgcttgat gatcggctca gcgatttgct taccgatttt ttgagtcggg
      241 ttcaaagaag tcatcggatc ttggaaaatc attgcgattt ctgaaccgcg gattttttgc
      301 atttctttct cggttttgtg gactaaatct tcgcctttga acaagatctc tccgtgttcg
      361 atgttggcgt tgtttgccaa cagacgcatg atactacggc tagtcactga tttaccagag
      421 ccggattcac caacgatggc taatgtttca cccggcttca ggtcaaaact gacgccacgg
      481 atagctttta ctttacctgc ataggtatca aaagtaattt gtaggtcttt tacttccaat
      541 atattttcca tctggttacc tcctaatctc tcatttttgg atcgaacgcg tcacgaagtc
      601 catcagccaa taagttgaag caaatcatga tgacgctcat gatgccggct ggataccaca
      661 tcagatgcgg caagaaacgg aatgttttgt agccatcgtt gatcaatgta ccaagcgatg
      721 cattcggagc gggaataccg ataccgataa agctcaggaa tgcttcgaag aagattgccg
      781 atggaatcga gaacataact tgaatgatga tgacgccagc caagttaggc aggatgtgcc
      841 tgaaggcgat tttcatcggt gactggccta acgtacgggc tgccaaaatg tattcttgat
      901 ttttcagttt taatgtttgc gctcgtacga cacgcgccat cgaaagccat tcagtcaatg
      961 ccagagcaat gatgnnnnnn nnnntcaaaa caagcagcat caaaataacg actaccaagt
     1021 tcggaacgcc ggaaaggatt tccagaatac gttgcatgat attgtcgact tttccgccca
     1081 accagccgga agtcagacca taaacaacac caagcgtcaa gttgaataaa gctgccataa
     1141 aagcaatgaa cagagacacg cgggtaccat acaggatccg cgacaacaaa tcgcgtccca
     1201 ggctgtccgt accgagatag taatagacgt cctctggaac attggatgct tcgtacttgt
     1261 cgatgactac gccattctgt ttcacggtcc cattcagtcc attgatgtcg atgccgggaa
     1321 tcttcggcgg catgttagca tactggacag tttgcgcatt cgggtcatgc ggtgcgatga
     1381 caggagccaa caaactggac atactgatga tgatcaagat gatcaagcta accatcgcaa
     1441 ccttgttttt cttcagacgg cgccaagaat ccgccatgaa actcagggaa ggcgctgaga
     1501 ttttttcttt atcctgcata cttgattcgg atgccgattc gaaaagatct ttcgtgattg
     1561 tcggcatagc tgaagtattg cgtaattcat tctcactc
//
LOCUS       DAOMUJ010000442         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21512_length_1595_cov_1.735714, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000442 DAOMUJ010000000
VERSION     DAOMUJ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21512_length_1595_cov_1.735714"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..690
                     /locus_tag="PLT73_09205"
     CDS             79..690
                     /locus_tag="PLT73_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRM20586.1"
                     /translation="MVKVKICGLKRVEDAEMLNELRPDFAGFIFAPSKRQVSLEQALE
                     IRAALDEAIPAVGVFVNAPIENIVAIVESRAIQIVQLHGDEDNAYIEELGKRITLPII
                     KAVAVKDAADVKKAYAADILLYDTYQSGVAGGTGKTFNWDLLKEATQPFFLAGGLHAG
                     NLEGAIKQMQPYAVDISSGVETDGVKDFEKIRAVMEIIGRSKK"
     gene            687..>1595
                     /gene="trpB"
                     /locus_tag="PLT73_09210"
     CDS             687..>1595
                     /gene="trpB"
                     /locus_tag="PLT73_09210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRM20587.1"
                     /translation="MTSYFGAHGGQFIPETLMNACLELSEAFFRLKEDPDFKKELNGL
                     LDNYAGRPSRLYYAEKMTKNLGGAKVYLKREDLNHTGSHKINNALGQVLLAKKMGKKR
                     IIAETGAGQHGVATATAAALLDMECVVYMGEEDTQRQALNVFRMELLGTKVIPVTSGT
                     ATLKDAVNEAMKDWASTFEESHYCIGSVMGPHPFPTMVRDFQKIIGEETKTQMAKAEG
                     KLPDVVMACVGGGSNAIGTFYDFIGDESVRLIGCEAAGRGIHTQDTAATIATGTPGIF
                     HGMKSYFCQDEYGQIAPVYSISAGLDY"
BASE COUNT          423 a          397 c          460 g          315 t
ORIGIN      
        1 tgcgatcctg atcggcgaaa cgatgatgat ttctccagac aaaaaaggca tgctcgatcg
       61 ccttcgggga atcggctgat ggttaaagta aaaatctgcg gtctgaaacg agtcgaggat
      121 gctgaaatgc tgaacgagct acgcccggat tttgcaggct tcatcttcgc tcccagcaag
      181 cgccaggttt cgttggaaca ggcgctggaa atcagggccg ccctagacga agccattccg
      241 gcagtcggcg tcttcgtcaa cgcaccgatc gaaaacatcg tcgcaatcgt ggagagccgg
      301 gcaatccaga tcgtgcagct acacggcgat gaggataacg cttatataga agagttgggc
      361 aagcggatca cacttccgat catcaaagcg gttgcggtca aggacgccgc tgatgtcaag
      421 aaggcatatg ctgccgatat tctcctctat gacacatacc aaagcggtgt ggcaggtgga
      481 accggaaaga cattcaactg ggatttgctg aaagaagcga cgcagccatt cttcctggcg
      541 ggcggcctgc acgccggcaa tcttgagggg gccatcaagc agatgcagcc atacgcagtg
      601 gacatctcga gcggcgtcga aacggacggc gtaaaggatt ttgaaaaaat cagagcagta
      661 atggaaatca ttgggaggtc aaaaaaatga caagttattt tggagcacat gggggacagt
      721 tcattcccga aaccttgatg aacgcttgtt tggaactatc ggaggctttt ttccgactca
      781 aagaagatcc ggacttcaaa aaagaattga acggtctgtt ggacaactac gcaggccggc
      841 catcccgcct ctattacgcg gaaaagatga caaagaacct cggcggagca aaagtctacc
      901 tgaaacgcga agacctgaac cacacgggct cgcataagat caacaatgcg ctcggccaag
      961 tattgttggc caaaaagatg ggcaagaaac gcattatcgc tgagaccggc gccggccagc
     1021 acggcgtggc gacggcaaca gcagctgcgc ttttggacat ggagtgcgtc gtctacatgg
     1081 gcgaagagga cacccagcgc caagccttga acgtgttccg aatggaattg ttgggcacga
     1141 aagtgattcc tgtgacaagc ggcactgcca cgcttaagga cgcggtcaac gaagcgatga
     1201 aggattgggc ttcaacgttc gaagagtcgc attactgcat cggctcggtc atggggccgc
     1261 atccattccc gaccatggtc cgcgatttcc agaaaatcat cggggaagag acaaaaactc
     1321 agatggccaa ggcggaaggc aagctgccgg atgtcgtgat ggcttgcgtt ggcggtggct
     1381 cgaatgcgat cgggactttc tatgacttca tcggcgacga aagcgtgcgc ttgatcggct
     1441 gcgaagctgc cggcagaggc atccacaccc aagatacggc tgcgacaatc gcgaccggta
     1501 cgccgggcat tttccacggc atgaaatcct atttctgcca ggatgaatac ggacagatcg
     1561 ctcctgttta ttcgatatcg gctggactgg actat
//
LOCUS       DAOMUJ010000443         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21528_length_1594_cov_1.815465, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000443 DAOMUJ010000000
VERSION     DAOMUJ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21528_length_1594_cov_1.815465"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..809
                     /locus_tag="PLT73_09215"
     CDS             117..809
                     /locus_tag="PLT73_09215"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRM20588.1"
                     /translation="MRPFREILSMAVKRHGSLEAVEARIEHPAPITDLLARSDDRWLA
                     DMTRHVFNAGFSRKVIRDKWPGFEAAFKGFDPSVCAIMDDDWFDMLLKDPRIVRNGAR
                     IRSVQRNAMFILDETERLASFGAFIAAWQHKDFADVLDYLRKHGDRLGDKTAQYFLRE
                     AGVDSYVLSFDVLKRLSLEGVADTLPASSKQRRAIQNAFDGWKEESGKSLTYISRVLA
                     MSVESDRQPRFT"
     gene            833..1300
                     /locus_tag="PLT73_09220"
     CDS             833..1300
                     /locus_tag="PLT73_09220"
                     /inference="COORDINATES: protein motif:HMM:NF018748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM20589.1"
                     /translation="MEQRQPLPPFTRETAIQKVRLAEDGWNGRNPEAVSKAYTVNTKW
                     RNRATFVNGRDEVVAFLADKWAKELDYRLVKELWAFTDNRIAVRYAYEYHHADGNWFR
                     AYGNENWEFDENGLMTNRHASINDLPIKESERLFHWPLGRRPDDTSSLSELGL"
     gene            1363..>1594
                     /locus_tag="PLT73_09225"
     CDS             1363..>1594
                     /locus_tag="PLT73_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20590.1"
                     /translation="MLALRNGRFISTTLSANSERMLGIATTLNIAVSDAEGRVWSFLA
                     NGPEGLVARADDKTVYLNRTEMTVPSALWTMVS"
BASE COUNT          378 a          422 c          459 g          335 t
ORIGIN      
        1 tgcctgcgtt ctattggaaa agactgaggc ctttccctag tctagtggag accggccggc
       61 acaccggaag tggccactct cgacatcccc ggaaccacat tcttgaagta ggcttgatga
      121 gacctttccg cgaaattctg tcgatggctg tgaagagaca cggcagtctt gaggccgttg
      181 aggcgcgcat tgaacacccg gcacccatca cagacctgct tgcacgcagt gacgatcgat
      241 ggctcgcaga tatgacacga cacgtcttca acgcgggctt cagccgtaag gtgatccgcg
      301 acaaatggcc gggattcgaa gcagctttca aagggtttga cccaagcgtt tgcgcaataa
      361 tggatgacga ctggttcgat atgctgctca aagatccgcg catcgtgcgc aacggggcgc
      421 ggatccgatc cgttcagcgc aacgcgatgt tcattctgga cgagacagaa agacttgcca
      481 gctttggcgc tttcatcgcc gcgtggcagc acaaggattt cgccgacgtt cttgattatc
      541 tgcgcaagca tggtgatcga ctgggcgaca agaccgccca gtatttcctg cgcgaagccg
      601 gtgtagattc ctatgttttg tcgtttgatg tcttgaaacg cctgtcactc gaaggggttg
      661 ctgacactct gccagcgtcg tcgaaacagc ggcgcgccat acagaacgcc tttgacggat
      721 ggaaagaaga gtcgggcaag tcgctgacat acatcagccg cgtgctcgcc atgagtgtcg
      781 aaagcgaccg ccagccaaga tttacataaa cattcccaga aaggatcaat gaatggaaca
      841 acgccaacct ctcccgccct ttacgcgtga gacagcaatt cagaaggtcc gccttgccga
      901 agatggctgg aacggtcgca atccagaggc agtatccaaa gcctatacag taaacaccaa
      961 atggcgcaac cgcgcgacat ttgtgaatgg tcgcgatgag gtggtagctt tcctagcgga
     1021 caaatgggcc aaggaattgg actaccgact ggtcaaggaa ctctgggcct tcacggataa
     1081 ccgcatcgcg gttcgatacg cgtatgaata ccaccatgct gatggaaact ggttccgcgc
     1141 ctatggcaac gagaattggg agtttgacga gaatggcctg atgacgaacc ggcacgccag
     1201 catcaatgat ctgccgatca aggaaagcga gcgtctgttc cactggcctt tgggacgccg
     1261 tcctgatgac acgtcaagtc tttctgaatt gggactgtga ggatgagcgg gggccctttt
     1321 cacgatggtg aacgccttgt ccagaaagcc acgggggagg acgtgctggc ccttcggaac
     1381 gggcgtttca tcagcacgac actgtctgca aacagcgaac ggatgctggg tatcgcgaca
     1441 accctgaata tcgccgtaag tgatgctgag gggcgtgtct ggtcttttct ggccaacggt
     1501 ccagaagggc ttgttgcccg agctgatgac aagacggttt acctgaatcg gaccgaaatg
     1561 accgttccgt ccgcgctatg gacgatggtc agtg
//
LOCUS       DAOMUJ010000444         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21554_length_1593_cov_1.524707, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000444 DAOMUJ010000000
VERSION     DAOMUJ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_1593_cov_1.524707"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>972)
                     /locus_tag="PLT73_09230"
     CDS             complement(66..>972)
                     /locus_tag="PLT73_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRM20591.1"
                     /translation="MHHATKQLRLRTLELTGKLLNLQIADSLRSAATAWTEGFNAKNG
                     ARKLAEAYIQALETTDTTDPEIRQLVNELTDYKDYLMKRSTWMIGGDGWAYDIGFGGI
                     DHVLASGEDVNILVLDTEVYSNTGGQVSKSSNLGAIAEFASGGKSVRKKDLGMMMMNY
                     GYVYVAQIAMGANPRHTLKVISEAEAYDGPSIVIAYSPCISHGIKCGLTVSQEHEKDA
                     VDVGYWHLYRFHPENKLKGKNPFKLDSKEPQFERFQDFLMEEVRYSSLYNQYDAEHVQ
                     AIFDESLLAAKDRYQSYTRLESYTD"
     assembly_gap    973..982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<983..>1593)
                     /locus_tag="PLT73_09235"
     CDS             complement(<983..>1593)
                     /locus_tag="PLT73_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRM20592.1"
                     /translation="YEKREIAVQIPIWNPEECIQCNQCSFVCPHAAIRPFLATEDEAA
                     NAPEGVEFLDGMRPYKDYKYRIQVSAADCTGCGICVLTCPARKKAIEMKNREDHLEEN
                     NNWDYMIRLPHKENPVGINTVKGSQFEQPLFEFSGACAGCGETPYIKLVTQVVGDRMQ
                     IANATGCTSIYGGSAPSTPYTTNDAGCGPSWANSLLEDNAEFG"
BASE COUNT          350 a          404 c          411 g          418 t
ORIGIN      
        1 ggttttcatt tatccaaaaa aaagaccagc cgccacaatt aggcggcgac tggtccggat
       61 tgaagctagt ccgtgtagga ttccaagcgt gtgtaggact gataacggtc ttttgcagcc
      121 aggaggctct catcaaagat agcctgtaca tgctcggcat catattggtt gtataaggaa
      181 gagtagcgaa cctcttccat caggaaatcc tggaaacgtt caaactgcgg ttccttagaa
      241 tccaacttga aggggttttt ccctttcagt ttgttttccg ggtggaagcg gtaaagatgc
      301 caataaccga catccactgc atctttttcg tgttcctgcg atacggtcaa accgcacttg
      361 atgccgtggc tgatgcatgg ggaataagcg atgacgatgg aaggcccatc ataggcctcc
      421 gcctcggaga tgactttcag cgtgtgtcta gggtttgcgc ccatcgcgat ctgggcgaca
      481 tagacatacc cgtaattcat catcatcatg cccaagtctt ttttgcggac ggatttgcca
      541 ccggaagcga actcggcgat ggcacccagg ttggacgatt tcgaaacctg tccgcctgtg
      601 ttggaataga cttcggtatc cagcacgagg atattcacat cttccccgct tgcgaggaca
      661 tggtcgattc cgccgaagcc gatatcatag gcccagccat cgccgccgat catccaggtg
      721 gaccgcttca tcagatagtc cttatagtcc gtcagttcat tgaccaattg gcgtatttcc
      781 ggatccgttg tgtccgtagt ttccaaagct tggatgtaag cttcagctaa tttgcgggcg
      841 ccatttttgg cgttgaaccc ttcagtccaa gccgttgcag ctgatctgag cgaatcagct
      901 atctgtaagt tcaaaagttt ccctgtcaat tcaagtgtgc gtaggcgtag ctgttttgtc
      961 gcatggtgca ttnnnnnnnn nnagccgaat tccgcattat cttccaaaag ggaatttgcc
     1021 caagacggtc cacagcctgc atcattggtt gtgtacggcg tggaaggagc agagccccca
     1081 tagatggacg tacaaccagt cgcattcgcg atctgcatcc gatcgcccac cacttgggtg
     1141 accagtttga tgtatggcgt ttcgccacag ccggcgcagg caccggaaaa ttcgaacaat
     1201 ggttgttcaa actggctgcc tttcaccgta ttgatgccga ccggattttc cttgtgcggc
     1261 aggcggatca tataatccca attattgttt tcttcaagat ggtcttcacg gtttttcatc
     1321 tcgatcgctt ttttgcgggc gggacaggtc aggacgcaga taccgcagcc cgtgcaatcg
     1381 gcagcggaaa cttggatccg gtatttatag tctttgtacg ggcgcatccc gtccaggaat
     1441 tccacacctt caggggcatt ggctgcctcg tcttctgtgg ccaggaacgg ccggatggcg
     1501 gcatgcggac aaacaaagga gcattgattg cactggatgc attcctcagg gttccagatc
     1561 ggtatctgta ccgcgatttc gcgtttctcg tat
//
LOCUS       DAOMUJ010000445         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21568_length_1592_cov_2.235524, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000445 DAOMUJ010000000
VERSION     DAOMUJ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21568_length_1592_cov_2.235524"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLT73_09240"
     CDS             complement(<1..436)
                     /locus_tag="PLT73_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM20593.1"
                     /translation="MTINQQQIHQEVTDIYDYIIALRRHFHRHPEPSLKEFETIQRIK
                     EEVEEIGVPYINVGETGILVTLEGGKGSGKTILLRADIDALPLPDETGKPYASVNPGF
                     NHACGHDGHTASLLGALKILKEHQDKFAGTIQFAFQPAEEIGA"
     gene            complement(519..1376)
                     /locus_tag="PLT73_09245"
     CDS             complement(519..1376)
                     /locus_tag="PLT73_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM20594.1"
                     /translation="MKNKTFLATLTLSASLFLAACGNAASDSASDSSTADANEPVKVT
                     LGVVGEVNEPWDYVIEELKEKENIEVELVKFTDYTTPNNALAEGEIDLSSFQTEIFMD
                     NYNRDHGTELTTIGYTVMAPLGLYSDKIKDISELKDGDTIAIPNDVSNEGRALILLQT
                     AGLIKLDPEAGLVPTTENVIENKLNLQFQTLESNQTARALQDVTASVINSGMAVDAGF
                     IPSEDAVFLEPVTEDSKPYYNVIAALSEDVDNEVFQTIVAYYQSEGTAKVIEESSKGS
                     QFPVWDEAK"
BASE COUNT          356 a          356 c          417 g          463 t
ORIGIN      
        1 cggcgccgat ttcttctgct ggttggaagg cgaactggat tgtcccggca aatttatcct
       61 gatgttcctt cagtattttc agcgcgccca acaaggaagc ggtatgcccg tcgtggccac
      121 aagcatggtt gaaaccggga ttgacggaag cgtagggttt accggtctca tccggcaaag
      181 gcaacgcatc gatatcggca cgcagcaaaa tggtcttgcc tgatcctttt ccgccctcca
      241 aggtgaccaa gataccggtc tccccgacat ttatgtaagg cacgccgatt tcttcgacct
      301 cttccttgat gcgttggatc gtttcgaatt ccttcaggct cggctccgga tgacggtgga
      361 aatggcggcg cagggcgatg atgtagtcgt agatatcggt aacttcttgg tgaatctgtt
      421 gttggttgat tgtcatggat gattcctcct tgaatcttta taggaaaggg gctttgggca
      481 aatggcccaa gcccctagta atggtttgat tatctatatt attttgcttc gtcccaaacc
      541 gggaactgcg agccttttga agattcttcg atgactttgg ccgttccttc ggattgatag
      601 taagctacga tcgtttggaa gacttcgttg tcgacatctt cggagagtgc cgcaatgacg
      661 ttgtaataag gttttgagtc ttccgtaacc ggttcaagga agacggcatc ttcactcggg
      721 atgaagccgg catccacggc catgccgctg ttgatgacag aagcggtcac gtcctgcaaa
      781 gcgcgagcag tctgattgga ttcaagcgtc tggaactgca gattcaattt gttctcgatg
      841 acattttcag tagtcggaac caaaccggct tcagggtcaa gcttgatcaa tccggcagtc
      901 tgaagcagaa tcaaagcgcg tccttcgttg gagacgtcgt tcgggatggc gatcgtgtcg
      961 ccgtctttca gttcgctgat atccttgatt ttatcggagt aaagtcctaa aggagccatt
     1021 actgtatagc cgatggtcgt caattctgtt ccatgatctc tgttgtagtt atccatgaag
     1081 atctctgtct ggaacgaact caggtcgatt tcaccttcgg ccaaagcatt gttaggagtt
     1141 gtgtagtcgg tgaatttgac cagttcgact tcgatgttct ctttttcttt cagctcttcg
     1201 atgacgtaat cccaaggctc attgacttcg ccgacgacgc ccaatgtgac tttgacgggc
     1261 tcattcgcgt ccgcagtgga agaatcgctg gcggaatcgg aggctgcatt gccgcaagcg
     1321 gcaaggaaaa ggctggctga aagggtcaag gttgctaaaa atgtcttatt tttcattagt
     1381 aaagggctcc tttgtatatg aaaatagcat caagagcggc gcgaaaagaa atgcaccggc
     1441 ttgatgctac tcttaaattt agtatgtttt cagggtgttg aagatgaaga aaggctttcg
     1501 cttagtccca agcagggata tctgtgttag cagttgtttc tgcgatcaaa gcttttgttt
     1561 cttctgtttg gtatgccgct acaacttttt tg
//
LOCUS       DAOMUJ010000446         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21611_length_1590_cov_1.237134, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000446 DAOMUJ010000000
VERSION     DAOMUJ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21611_length_1590_cov_1.237134"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLT73_09250"
     CDS             <1..963
                     /locus_tag="PLT73_09250"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HRM20595.1"
                     /translation="IADNLDNENDSELLEMSADSTKTVSNHDTLSDPITNQMTSGGNQ
                     PSLIAEDDVFAFIEIPKIGEMLPIFLGATPKNLAKGVAQVTESSLPTGGSGTHSVIAG
                     HRGYYGATFFRYIDQMEIGDLFYIHVLNKVLAYEVTGQAIIEPDDTSSLGIVETEDLV
                     TLLTCTPYPTDRYRLLIYGSRVYDEGGTVEYASASEDPLIGKTNTEDAEIGSAYPDAQ
                     GQVLDEQTSYAEDNTEIVENRDSDEAIEENTVLTGDKDETQPSNEETSVSSSENVSVQ
                     LLQSETDISTTARNHKWINRLIIIAGLLSIAVSIGLIVKNKRKG"
     gene            1203..>1590
                     /locus_tag="PLT73_09255"
     CDS             1203..>1590
                     /locus_tag="PLT73_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20596.1"
                     /translation="MDKKINQLIEIAEFAMEANDYAHAEKKYINALYLMDNPKSEEYQ
                     KVVNKLAKCYAAQKNFAGAKECIEELLFYAKKNKDLKKESEYLHALAANTRCMKEYDL
                     AALMCEEEITFRLTHFPDDYGGLARSY"
BASE COUNT          508 a          289 c          376 g          417 t
ORIGIN      
        1 attgctgata atctggataa tgaaaatgat tccgaactcc tggaaatgtc tgcagacagc
       61 accaaaaccg tgagtaatca tgatacgctg agcgatccaa ttaccaacca gatgacttcc
      121 ggcgggaatc aacctagttt gattgctgaa gatgatgttt ttgctttcat cgaaataccg
      181 aaaattggtg agatgttgcc tatttttcta ggagccactc ctaaaaatct tgcaaaaggc
      241 gttgctcaag taacagagtc atcgttgccg acgggcggat ccggcacgca ctcggtcatt
      301 gccggtcatc gaggatatta tggtgcaaca tttttcagat atatcgatca aatggaaatt
      361 ggggatttgt tttatataca tgtattgaat aaggtgctag catacgaggt cacaggacaa
      421 gctataatcg aacctgacga tacctcttcg ctaggtattg tcgaaacaga ggacttagta
      481 actttactga catgcacgcc ttatccgaca gaccgatata gactgcttat ctatggatcg
      541 agggtatatg atgagggagg aactgttgag tatgcaagcg cctcagaaga cccactaatc
      601 ggtaaaacca atacggaaga tgcggaaatt ggctcggcgt accctgatgc gcaggggcaa
      661 gttttagatg aacaaacatc ctatgccgag gacaatacgg agattgtaga aaaccgggat
      721 agcgatgaag ccattgaaga gaacactgtt ttaacagggg acaaggatga aacacagcca
      781 agtaacgagg agacttccgt gtcttcatca gaaaatgttt ctgttcaact tttacagtcc
      841 gaaacagaca tttcaacaac cgcccgcaat cataaatgga tcaatcgttt gattatcatt
      901 gcaggcttat tgagtattgc tgtcagtatc ggattgattg tgaaaaataa gagaaaagga
      961 taatgaagca tatttttcga acgaatgtaa cgcatgaaag aatttaaaaa acttttgagg
     1021 tattgatgat ggcgctctgg aatagggcgc cattttcatt cactgagtta ggtgatgcgt
     1081 atttctgaag ggaggcacat ctaatagtgt tgctatcgtt atcctttgtg aaaccaccca
     1141 aatggtgtta agatggtgat gaaaacgata acattttttt cgtgaagcaa gggggatgcg
     1201 cgatggacaa gaaaattaat caactgattg aaatcgctga atttgcgatg gaggcgaacg
     1261 attatgctca tgctgaaaaa aagtacatca acgccttata tttaatggat aatcctaaat
     1321 cggaagaata tcaaaaagtc gtaaacaagc tggcgaagtg ctatgccgcc cagaaaaatt
     1381 ttgctggagc caaagagtgt attgaagagc tattgtttta cgctaaaaaa aataaagatc
     1441 tgaagaaaga atcagagtat ctgcatgcct tggcggcgaa tacccggtgc atgaaagaat
     1501 acgatttggc agcattgatg tgtgaagaag aaatcacttt ccgattgaca cattttcccg
     1561 atgattacgg tgggttggcc cgcagttact
//
LOCUS       DAOMUJ010000447         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21809_length_1579_cov_1.479659, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000447 DAOMUJ010000000
VERSION     DAOMUJ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21809_length_1579_cov_1.479659"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1211
                     /locus_tag="PLT73_09260"
     CDS             153..1211
                     /locus_tag="PLT73_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM20597.1"
                     /translation="MTELFMKLLNMSINAGWLVLAVLLVRIIFRKAPKWLFPLLWSFV
                     WIRLLMPNALESRLSVIPDISGFSASEQGLLSAKGFTAPVPYVGIVWCTGMAAMLLFE
                     ALSYVHLRSKVKTAVRLSSNVYQSEHVNAPFILGLFAPRIYIPFHIKSKELDSIIAHE
                     RAHIKRRDHWLKPIAFLILSLHWFNPLIWIAYYFLCKDIELACDEYAIKDMSDSQRAA
                     YSQTLLSCTANQRIFAACPLAFGSKNVKTRIRAVLSYRKPHAWIITVTAILVLAVVAC
                     FMTNPRQSKESTAQGSNYIDLNQPPTLDVIQNGQTISDRSSSEDRQREVELENALLGE
                     AIKKQESLTQIQTTDFYR"
     gene            1355..>1579
                     /locus_tag="PLT73_09265"
     CDS             1355..>1579
                     /locus_tag="PLT73_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20598.1"
                     /translation="MQKIKSLAAVFLALFILAAIPTQAFAAETHEEVATMHTHQWRLD
                     HYDTTYIPIDDETHLKTVYPVYYCTVSGCTN"
BASE COUNT          425 a          337 c          360 g          457 t
ORIGIN      
        1 acagacagct gaaattgagg aattggtcga gaaaaaattt gccggttcga ttccagcttt
       61 tttgaccgca tttaccaaaa gccaacagct cacggcagat gaaattcagg agatgcagca
      121 gatgattgac cgctttcgga aggagcatcc ggatgactga attgtttatg aagctgctta
      181 atatgagcat caatgccgga tggcttgtac tggctgtcct acttgtccgc atcatattcc
      241 gcaaggctcc aaaatggctg ttccccttat tgtggagctt tgtttggatc cggcttctca
      301 tgccaaacgc attggaaagt cgcttgagtg tcattcctga tataagtggc ttttcggcat
      361 ccgagcaggg cctgctttct gctaaaggtt tcactgctcc tgttccttat gttggaatcg
      421 tctggtgtac tggtatggca gcaatgcttc tgtttgaagc attaagctat gtacatctcc
      481 gcagcaaagt aaaaactgct gtccggctct cgtcaaatgt gtatcaaagc gagcatgtga
      541 atgctccgtt tattcttggc ctgtttgcgc cgcgcatcta tattccgttc catataaaga
      601 gcaaggaatt ggatagcata attgcacatg aacgtgcgca tataaaacgc cgcgatcatt
      661 ggctaaagcc cattgcgttc ttaattcttt ctcttcattg gttcaatcct ttgatttgga
      721 ttgcgtatta tttcctttgt aaggatattg agttggcatg tgacgaatac gctatcaagg
      781 atatgagtga ttctcagcgc gctgcgtatt cgcagacgtt gctctcctgc actgcaaacc
      841 agcgtatttt tgcggcctgc ccgctggcct ttggcagtaa gaatgtgaaa acacgaatcc
      901 gtgctgtact ttcttatcga aagcctcatg cttggatcat aaccgtgacc gcgatacttg
      961 ttttggcagt tgtcgcctgt tttatgacaa acccgagaca atcaaaagaa agtactgctc
     1021 agggtagcaa ctatattgat ctaaatcagc ctccaacatt ggacgtgatt caaaatggac
     1081 aaactatttc ggatagaagt agtagcgaag atcggcaacg tgaagtagag cttgagaacg
     1141 ctttgttggg agaagcgata aagaagcaag agtctcttac tcaaattcaa acaactgatt
     1201 tttacagatg aaaagtcctg cgttatctat aggattgacg tcaatttaac tgtcgatgtg
     1261 gttacatgga aggagggaag attcatgaaa atcatttgag gaagcacatg agccagaatc
     1321 cgacaaaaaa tcagttaaaa aaggaacgtt gtttatgcaa aagatcaagt cactggccgc
     1381 agtttttctg gcccttttta tcctcgcagc gattccgaca caggctttcg ccgctgaaac
     1441 tcatgaagaa gttgccacaa tgcataccca tcagtggcgt cttgaccatt atgatactac
     1501 atatattccg attgatgatg aaactcatct gaagactgtc tatcccgtgt attactgcac
     1561 tgtttcgggg tgcactaat
//
LOCUS       DAOMUJ010000448         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21820_length_1578_cov_1.780696, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000448 DAOMUJ010000000
VERSION     DAOMUJ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21820_length_1578_cov_1.780696"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..>562
                     /locus_tag="PLT73_09270"
     CDS             148..>562
                     /locus_tag="PLT73_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM20599.1"
                     /translation="MIELIVSDMDGTLLNDKMEVSASNVSAINEANRLGIKFMVATGR
                     GYAEAVPALKEVGIDCPMITLNGGQVFDEDGQLVENLGIDKTTTRRILEDVKKYGLYC
                     EIMTSKGIFSDDKVRRIESMTSLLHETNPDTTFKMA"
     assembly_gap    563..572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <573..1027
                     /locus_tag="PLT73_09275"
     CDS             <573..1027
                     /locus_tag="PLT73_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010826433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM20600.1"
                     /translation="VLAVARLEVMHVNYVDDFKDVLDDNTQQVLKILVFSDSGQGELE
                     PIRTKLAEDSRLVVTSSFRNNIEINHVEAQKGIALEKFAKKRGIPLENVMAIGDNFND
                     VSMLKVAGASFAVANAEDGVKVFAKHLTSKNSENGVAEAIMRCINENL"
     gene            1106..1258
                     /locus_tag="PLT73_09280"
     CDS             1106..1258
                     /locus_tag="PLT73_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HRM20601.1"
                     /translation="MDANVDVLAYMELISNTGFPIVIAMYLLHRMEKKLDALVGAIEE
                     LSRAFK"
BASE COUNT          470 a          324 c          366 g          408 t
ORIGIN      
        1 taccatatta ggcggacctt tttcagtcat tccgcctact ggcatattgt ttatgcagtt
       61 cttgaaacta atttttaacc aaaagtgaaa agcgtggtag aatataagtg tgaatatact
      121 gcaattaccg aacaggagga tgaatatatg attgaactga tcgtctcgga tatggacggc
      181 accttattga acgataagat ggaggtctcc gcatcgaacg tgagcgccat caacgaagca
      241 aatagattag gcatcaaatt tatggtcgct accggcagag gatatgctga agctgttccg
      301 gccctcaagg aagttggcat cgactgccct atgatcacgc ttaacggcgg gcaagttttt
      361 gatgaagatg gacagttggt cgaaaatttg ggaatcgata aaacaaccac acgccgtatt
      421 ttggaagatg ttaaaaaata tggtctttac tgcgaaatca tgacatcaaa agggatcttt
      481 tcggatgaca aagtgcgccg gatcgagtca atgacttcct tgctgcacga gacaaaccct
      541 gacacaacat tcaaaatggc gcnnnnnnnn nnttgtcttg gcggtcgccc gtttggaagt
      601 gatgcacgtc aactatgtcg acgacttcaa agacgttctg gatgacaata cccaacaagt
      661 attgaaaatc cttgtattca gcgattccgg acaaggcgaa ttggagccga tccggacaaa
      721 gttggcagag gacagccggt tggttgtgac ctcctccttc cgaaacaaca tcgaaatcaa
      781 ccatgtggaa gcacaaaaag ggattgcttt ggaaaagttc gccaaaaaaa gagggattcc
      841 cttggagaat gtcatggcaa tcggagacaa cttcaatgat gtctcgatgt tgaaagtagc
      901 cggcgccagc ttcgcagtgg ccaatgctga agatggcgtc aaagtatttg caaagcacct
      961 cacttccaaa aactcagaaa acggcgttgc cgaagccatc atgcgctgca tcaacgaaaa
     1021 tctgtgatac cgaaaacgga acactgacag ttacacttcc ctcctcacct tcgtctttat
     1081 ggatgtagga aaggagggaa caggaatgga tgcgaacgtg gatgtgttgg catatatgga
     1141 actgatcagc aataccgggt ttccgatcgt aatcgcgatg tacctattgc accggatgga
     1201 aaaaaagctc gatgcacttg tcggcgcgat tgaagaactg agtcgggctt tcaaatgatc
     1261 acaacaatgc ctcaaaaagt atagaaagag ccctgacatg acagctttta tgctatcatg
     1321 ccagagcttt ttgctgcgtt ttttacgaac gttcccaaat aagccgaacc gtttgacact
     1381 cctatgtcag cgtttttatg tcatctggtt ttcatgtcac atttcctaac atgttcacgt
     1441 cgctataaga ttgtgctttc tgtgaattta aaatataatc tcgatgtggt attatatgaa
     1501 tattcaagca gaggcttcat cgtaatctca tggttattcg gaagaaatgc tgttttataa
     1561 ggaggaaaaa atatgtta
//
LOCUS       DAOMUJ010000449         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21856_length_1575_cov_2.412500, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000449 DAOMUJ010000000
VERSION     DAOMUJ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21856_length_1575_cov_2.412500"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PLT73_09285"
     CDS             complement(<1..1179)
                     /locus_tag="PLT73_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRM20602.1"
                     /translation="MHVLIYSIALILIIGFLFSSLFIRLQLPGLLGLILTGILLGPFG
                     MDLMAPELLAISADIRKIALIVILVRAGLTMELGDLKKIGRPAILMTFLPATFEILAV
                     TLLAPKLLGITLLESAILGTVLGAVSPAVVVPRMIMLIESGYGKAKGIPQMILAGASV
                     DDVYVIVLFTSFMGMYTGSGFQAITLLSVPVSIALGLLGGLLVGLGLVFLFRTFHIRD
                     TMKVMLVLSASFLLVTAEDSLAKFIPFSGLLGVMAVGATLLKRRPELAKRLNDKFAKI
                     WSAAEIFLFVLVGSAVDISALGSVGFAAVTLIFGALFFRMVAVILAVSSTNLNTKERI
                     FSAMAYIPKATVQAAIGSIPLAAGVEAGSIILSVAVLAIILTAPIGATFIDRTYKTFL
                     A"
     regulatory      complement(1222..1297)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT          491 a          387 c          368 g          329 t
ORIGIN      
        1 agccaggaac gtcttgtaag ttcggtcaat aaaggtggct ccgatcggtg cggtcaggat
       61 gatcgccaaa acggcgacgc tcaaaatgat actgcctgct tcgactccgg cagccaaagg
      121 aatcgatccg atggcagctt ggacagtggc tttcggtata tacgccatag cggaaaaaat
      181 gcgttccttt gtattcagat ttgtacttga aacggccaga atgaccgcca ccatccggaa
      241 aaataaagcc ccgaaaatca aagtaaccgc tgcgaaacca acactgccca aggccgaaat
      301 atcaacggct gatccaacca acacgaatag gaagatttcg gctgcagacc aaattttggc
      361 gaatttatca ttcagacgtt tggccaattc cggacgtctt ttcaataaag tcgctccgac
      421 agccatgacg cccaacaagc cggaaaacgg aatgaatttt gccaaggaat cctcagccgt
      481 caccaacagg aagctggcgc tcagtaccag catcactttc atcgtatcgc ggatgtggaa
      541 tgttctgaag agaaagacca atcccaagcc gaccagtagg ccgccgagca gacccaacgc
      601 gatcgaaacc ggaacggaaa gcagcgtaat tgcctggaac ccgctgcctg tgtacatgcc
      661 catgaatgag gtaaacagca cgatgacata aacatcatca acggaagcac ccgccagaat
      721 catctgtgga atccctttag ccttgccgta gccgctttcg atcaacatga tcatacgcgg
      781 gacaacgaca gccggtgata ctgcacccag aacagtacca aggatggcgg actccaataa
      841 tgtgatgccc aataatttcg gcgccaacaa ggtgacagcc aagatctcga aagttgcagg
      901 caaaaaggtc atcaagatag ccgggcggcc gattttcttg agatcgccca gctccatggt
      961 caaacctgcc cggacgagaa tgacaatcag agcaattttt cggatgtccg cagaaatggc
     1021 caacaattcg ggcgccataa ggtccatgcc aaaaggaccc aagaggatac cggtcaaaat
     1081 caagcccaac aagcctggca gttgcaatcg gataaagagc gaactgaaca gaaaaccgat
     1141 tatgagaata agagcgatac tgtagatcaa aacgtgcacc tccaaatttt tccactaaaa
     1201 aagctgacga cggactgcgc ctattgcgca gaagtcatca gcttatataa gcggtttaag
     1261 cgatttgctt cgggagacat tcattcccgt tagtataggt aatatgatag catgttttcg
     1321 gaagaccgac aagctgaaag aggacgccac attcttgttt tggcgctatt cccataactt
     1381 attatcctgt tatgggagat aatgtcaaaa ttgcgataat tgttaaaaac tgatatgata
     1441 aaagcaatca aattgaacgc ctcggggtca tcgactaata tttgtgggct agtcgaattt
     1501 tccaaacggc ctttccgaca agattttcca tcagaaaaat gtgaggtggc atgcgtgaac
     1561 caagtgaaca aagac
//
LOCUS       DAOMUJ010000450         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_21887_length_1573_cov_1.906456, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000450 DAOMUJ010000000
VERSION     DAOMUJ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21887_length_1573_cov_1.906456"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..325
                     /locus_tag="PLT73_09290"
     CDS             41..325
                     /locus_tag="PLT73_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRM20603.1"
                     /translation="MKTRKIPMRKCVVSNDMKPKKELVRIVKNQAGEIAIDPTGRMNG
                     RGAYVSMEPALVQKAWKQHILEKHLEIAISDAFYQELFDYVEHQKARSLL"
     gene            322..630
                     /locus_tag="PLT73_09295"
     CDS             322..630
                     /locus_tag="PLT73_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRM20604.1"
                     /translation="MTPEDKMLNLLGLSLRAGKLITGEEMTIKSIQKNEAVFVLCATD
                     CSTNTKEKLENKCRYYEVPFLVHFTSEQISQAIGKSRSICALTDRGFAKSFMRLKGTK
                     "
     gene            652..>1573
                     /locus_tag="PLT73_09300"
     CDS             652..>1573
                     /locus_tag="PLT73_09300"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRM20605.1"
                     /translation="MEKKRVYEYAKEHKVSSKTVLDKAKQLGIDYHSHMSTMEDGDIK
                     KLNQSISSTAGNQVAKPSAPQSNATQKKQPAENKKAPATRTHKTTDKSDLRKNTDMKN
                     KPSNPSSNQNNGSENKGQAKTTADKNAKRPAQATSQAGQNRPNTKMNRVDSVSPNKSN
                     DKPVQKTANTNTTTNAPAKSNRPSYGQNSGGSNPRSQGNQGRGNNPYNKNKRSKFKKG
                     TTKQGPAVPPRKFRELPETFVYTDGMNVMEVAKKLHREPAEIIKKLFLMGIMVTQNQA
                     LGKDALELLAADYGIEAEEKIVQDISDLDSY"
BASE COUNT          586 a          343 c          337 g          307 t
ORIGIN      
        1 aacaaaggac tttcaagact aatcagtgag gtgaaagttt atgaagacaa gaaaaattcc
       61 gatgcggaaa tgcgtcgtct ccaatgatat gaagccaaaa aaagaattag tccgcatcgt
      121 aaaaaatcaa gcaggtgaaa tagccatcga tcccacaggc cgcatgaatg gccgaggagc
      181 ctatgtctct atggaacctg cattggtaca gaaggcctgg aaacagcata ttttggaaaa
      241 acaccttgag atcgcgatca gtgatgcgtt ctatcaggag ctgttcgatt atgtcgaaca
      301 ccaaaaagca aggagcctgc tatgacacct gaagacaaga tgttgaattt gctgggtttg
      361 tcattgcgcg ccggcaagtt gatcacaggt gaagaaatga ccatcaagtc catccaaaaa
      421 aatgaagcag tctttgtgct ttgtgcgaca gactgcagca ctaacaccaa agaaaaatta
      481 gaaaacaaat gccgctatta tgaagtcccg ttcctggtgc atttcacttc ggaacaaatc
      541 agtcaagcaa taggcaagtc ccgttccatt tgcgccttga ctgatagagg attcgccaaa
      601 agtttcatgc ggctgaaagg aacaaagtaa tgagataagg aaggtgatga catggaaaaa
      661 aaacgtgtct acgaatatgc aaaagaacac aaggtatcaa gcaaaacggt gttggataaa
      721 gcaaaacaat tggggatcga ttatcatagc catatgtcaa ccatggaaga cggagacatc
      781 aaaaagttga accaatccat ctcttccact gctggaaatc aggttgccaa gccgagtgca
      841 ccgcaatcca atgcgacaca gaaaaagcaa ccagcagaaa acaaaaaggc gcctgcaaca
      901 agaacacata aaactactga taagagtgat ctgaggaaaa acacagacat gaaaaataaa
      961 ccatcgaatc cttcttcgaa tcaaaacaac ggttccgaaa ataaaggaca agcaaaaact
     1021 actgcagata aaaatgcaaa acggccggcg caagccacaa gccaagctgg acaaaatcgt
     1081 ccaaatacta aaatgaatcg agttgactct gtttcgccaa acaaatcaaa tgacaaacca
     1141 gtgcagaaaa ctgcaaacac gaataccact accaatgcgc cagccaaatc aaaccgtcca
     1201 tcatacggtc aaaattccgg cggttccaat ccgcgcagcc aaggaaatca agggagaggc
     1261 aacaatccct acaacaaaaa caaacgctcg aagttcaaaa aaggcacaac gaagcaagga
     1321 ccggcagtac ctccacgcaa attccgcgag ttaccggaaa ctttcgtcta tacagatggc
     1381 atgaatgtaa tggaagttgc caaaaagttg caccgtgaac cagctgaaat catcaagaag
     1441 ttattcctga tgggcatcat ggttacacaa aaccaagcac taggaaaaga cgctttggaa
     1501 ttattggctg ccgattatgg catcgaagct gaagaaaaaa tcgtgcagga tatttccgat
     1561 ttggatagct att
//
LOCUS       DAOMUJ010000451         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22073_length_1563_cov_2.477454, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000451 DAOMUJ010000000
VERSION     DAOMUJ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22073_length_1563_cov_2.477454"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PLT73_09305"
     CDS             <1..1099
                     /locus_tag="PLT73_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM20606.1"
                     /translation="GMKVLKTFGQEQEDIAELQVVIAQREEQLDEQARSVQVNGSNEN
                     YLNFIVASESFTDLVSRIDVVSKMVSANKELVEQQIADQKAVEDKKSKTEENLSKINA
                     MAMELEQLKGDLQVKKIEQESAIAALAAEKATAESDRQMFLAQKEEADQRAAAEAAAI
                     AAAEAAAATVAATVAQASSEESSTAASSVDIASSTVASSESAAPAQTVAAAPVVESAP
                     VVSAPAAAAPVVSAPVVSAPVVTAPTLSAPTGDVVSIAAKYIGVPYVWGGKTPSGFDC
                     SGFTSYVFREAYGIEIGGYTVPQENSGTQIAVSSAQAGDLIFWGSRGATYHVAIYVGG
                     GQYIHAPAPGQTVTYSSYNLSGASFAVRVAR"
     gene            1219..>1563
                     /locus_tag="PLT73_09310"
     CDS             1219..>1563
                     /locus_tag="PLT73_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM20607.1"
                     /translation="MEYTIQKLSRLAGVSTRTLRFYDEIGLLKPKRTSSSGYRIYGET
                     EVDRLQQILFFKQLSFSLDKIKAAMDDPAYDAEQSLVAHKQALLEKKKEIELLIKTVD
                     ATLEEKRGGRRMS"
BASE COUNT          461 a          356 c          387 g          359 t
ORIGIN      
        1 aggcatgaaa gtcttgaaga cattcggtca agagcaagaa gatattgccg aattgcaagt
       61 tgtcatcgca caacgcgaag agcaactgga tgaacaagca agatctgttc aagtgaacgg
      121 ttcaaacgaa aactacttga actttatcgt agcatccgaa tctttcacag atttagtatc
      181 ccgcatcgat gttgtttcaa aaatggtatc agccaataaa gaattggttg aacagcaaat
      241 tgcggatcaa aaagctgttg aagataagaa aagtaaaact gaagaaaact taagcaaaat
      301 taatgcaatg gctatggagt tggaacaact taaaggcgat ttgcaagtga agaaaatcga
      361 acaggaaagc gcaattgccg ctttggctgc tgaaaaagca acggctgaat ccgaccgtca
      421 aatgttctta gcgcaaaaag aagaagctga ccaaagagcc gccgcagaag ctgctgccat
      481 tgccgctgct gaagctgccg cagcaactgt agctgcaaca gtcgcacaag caagctcaga
      541 agaatcttca accgctgctt catccgttga tattgcatca agcacagttg caagttccga
      601 gtcagctgct cctgctcaaa cagttgccgc tgcaccagtt gttgaaagtg caccagttgt
      661 gtctgctcct gcagcagctg caccagtcgt cagcgcacca gttgtgtctg cgccagtcgt
      721 gactgcacca acattatcgg caccaacagg cgatgttgtt tccattgccg caaaatacat
      781 cggcgttcct tatgtatggg gcggaaaaac accgtcaggc tttgactgct ctggcttcac
      841 gtcttatgtc ttcagagaag catacggcat cgaaatcggt ggctacacag ttcctcaaga
      901 aaactcaggt acacagattg ctgtatcatc cgcacaagcg ggagatttga tcttctgggg
      961 ttcaagaggc gcgacttatc acgttgcgat ctacgtaggc ggcggacaat acatccacgc
     1021 tccggcacca ggccaaacgg ttacttattc ttcttacaac ctttccggcg catcatttgc
     1081 cgtacgtgtt gcaagataat ccaaaattca cgacagtcct ccggggctgt ctttttttga
     1141 tttttgcgta gtatgaagag aagctgttga ccttaacgca gcgtgaaggt gtaagatagg
     1201 atcatcggag gggatagaat ggaatataca atccaaaaat tgagccgatt ggccggcgtc
     1261 agcacgagga cgctgcgctt ctatgacgaa atcggcctgc tgaagcccaa acgcaccagt
     1321 tcctctggct accgcatcta tggtgagaca gaggtggaca ggctgcagca gatcctgttc
     1381 ttcaaacagt tgtctttttc gcttgataag atcaaagccg cgatggatga tccggcatat
     1441 gatgcggaac aatcattggt cgcacataaa caggcgctgt tggagaaaaa gaaggaaatc
     1501 gaattgctga tcaaaacggt ggatgctacg cttgaagaaa aaagaggagg cagaaggatg
     1561 agc
//
LOCUS       DAOMUJ010000452         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22095_length_1562_cov_1.873922, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000452 DAOMUJ010000000
VERSION     DAOMUJ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22095_length_1562_cov_1.873922"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PLT73_09315"
     CDS             complement(<1..1483)
                     /locus_tag="PLT73_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRM20608.1"
                     /translation="MKYSLTWDLDSFFEGGSSSPDLQDKIKSIQDGLKDFESAANNWN
                     PERDKPEYSDLKDLLHYEEQYTKALMQCNSFITGIQSADVSDLNASAIHARIIQLYSK
                     LTIIQTTLKKKITAIEENDWTLLVKMPAFSVLAFALSEWRQQGSRLLSDKEEALLANL
                     RVDGFNGWSAHYDTLVSFIKIPFEEADGTLNELSAGQAMNRMYADPDPSVRRKIFVEW
                     EKAWGYLAPAFADTLNHLQGFRLADFEAHQDEDFLVEPLRYNRIKRETLDTMWQAIST
                     NKQPFLSFLDRKAALMGKDRLAWYDVDAPVSLGSFQSKTFSFDEAVDYIIEHFASFGP
                     KLADFSLDAVKNRWVEAENRSGKRPGGYCEDYPESKESRIFMTFTGSASDMSTLAHEL
                     GHAFHSHVMRDLPDLNTRYAMNVAETASTFAETIISAATVREASSDEEKISLLNTKLE
                     NATAMFLNIHARYLFEKTFYTERMNGFVTAERLSVMMEAAQKEA"
BASE COUNT          371 a          372 c          370 g          449 t
ORIGIN      
        1 aagcctcttt ttgggcggct tccatcatta ctgacagtcg ttctgcagtg acaaagccgt
       61 tcatcctttc ggtgtagaag gtcttctcga aaagataacg agcatgtata ttcagaaaca
      121 tggccgtcgc attttcgagc ttcgtgttga gcaaggaaat tttttcttca tcgctgctcg
      181 cttccctgac tgtggcagcg ctgatgatgg tttccgcaaa agtcgatgcc gtttcggcga
      241 cgttcatggc gtagcgcgtg ttcagatccg gcagatcgcg catcacgtgt gaatggaagg
      301 catgtcccaa ttcatgggcc agtgtactca tatcgctggc tgaacccgta aaggtcatga
      361 aaatccgtga ctctttcgat tccgggtaat cttcgcaata gcctccaggt cttttgcctg
      421 atcggttctc ggcttctacc cagcggtttt tgacggcgtc caaggaaaag tctgccagtt
      481 tcggaccgaa ggaggcaaag tgttcgatga tgtaatcgac tgcttcgtcg aagctgaagg
      541 ttttcgactg gaaactgccg agcgacacag gggcatcgac atcataccaa gccagcctgt
      601 ctttgcccat cagagcggct ttgcggtcca aaaatgaaag gaagggctgt ttgttcgtac
      661 tgatggcttg ccacatcgta tccagtgtct cgcgtttgat gcggttgtaa cgcaatggct
      721 ccaccaagaa gtcctcatcc tgatgcgcct cgaaatccgc caaacggaaa ccttgcaggt
      781 gattcagcgt atcggcgaag gctggagcaa ggtatcccca agctttttcc cactcgacga
      841 atatttttct gcggacgctc ggatcgggat cggcatacat ccggttcatc gcttgaccag
      901 ctgatagttc gttcaaagtt ccgtcagctt cttcaaaagg aatcttgatg aaggatacga
      961 gcgtgtcata gtgcgcgctc cagccattga atccatcgac acgcaaattt gccaataagg
     1021 cttcttcctt atcggagagg aggcgactgc cctgttggcg ccattctgac agtgcaaatg
     1081 caagaactga aaaggcaggc attttcacca acaacgtcca atcgttttct tcgatggcag
     1141 ttattttttt cttcagagtc gtttgaataa tcgttaattt gctgtacaat tgaattattc
     1201 gcgcatggat tgcgcttgca ttcaaatccg atacatcggc ggattggata cctgttatga
     1261 aactgttgca ttgcatcagg gcttttgtgt actgttcctc gtaatgaagc aaatccttca
     1321 aatcagagta ttccggttta tcacgttcag ggttccaatt gtttgctgcg gattcaaaat
     1381 ccttcaatcc gtcttggatg gattttatct tgtcttgtag gtccggtgaa gaactcccgc
     1441 cttcgaaaaa cgagtccaaa tcccaggtaa ggctatattt catgctgatc atcctctcag
     1501 ttttgattga ctaatttatt gccataagta cattatatcc gttttacatc agaaataata
     1561 aa
//
LOCUS       DAOMUJ010000453         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22148_length_1559_cov_2.157580, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000453 DAOMUJ010000000
VERSION     DAOMUJ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22148_length_1559_cov_2.157580"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..698)
                     /locus_tag="PLT73_09320"
     CDS             complement(36..698)
                     /locus_tag="PLT73_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM20609.1"
                     /translation="MGLLLFDFDGTLAETCKISEMATMDTFEQFGYPAPTLEQLREDR
                     GKPHFVKFTGYLKQYALSPVEMDKLMEVFWDKYEKYENMLLEPYDLSRESLMELKGNG
                     HRLGVISNKASKALLRSLAAVDLLDLMEVVIGLENMDQHKPNPEGIFTACEKIPMAKE
                     ETVMIGDTIYDMQMGKMAGVKTVGITWGGSSAQTLRAENPDRIANCFVELEIIIASLL
                     DA"
     gene            1048..1404
                     /locus_tag="PLT73_09325"
     CDS             1048..1404
                     /locus_tag="PLT73_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM20610.1"
                     /translation="MAQKKELRRQFEAVPIESGIYEVQNTENQKIFIGKTPNLKTLNG
                     MKHILNVGANNNKELQSDWSHFGQDAFIFTILEVLEKSDDPSFNEKKALEQLEEKWLS
                     DKQPFGERGYNRRPKA"
BASE COUNT          429 a          377 c          346 g          407 t
ORIGIN      
        1 tccagaataa aggacaacgg ctttttccaa tctatttaag catcaagcag gcttgcgatg
       61 atgatttcca gttccacaaa acagttggcg atgcgatccg gattttccgc gcgcaaggtt
      121 tgggcggaac tccctcccca ggtgatgccg acggtcttta cgcctgccat tttccccatc
      181 tgcatatcat aaatggtatc accgatcatc acggtttctt ctttcgccat cgggattttc
      241 tcgcaagcgg tgaagatgcc ttccggattc ggtttgtgct ggtccatgtt ttccaatccg
      301 atgaccactt ccatcaggtc caacagatct actgcagcaa ggctgcgcaa caaagccttg
      361 cttgctttgt tggaaatcac gcccagacgg tggccgttcc ctttcagttc cattaaggac
      421 tcccgcgata aatcatatgg ttccaacagc atattttcat atttttcgta tttgtcccaa
      481 aagacttcca tcagcttgtc catctcgacc ggagagagtg cgtactgctt cagatagccg
      541 gtaaacttca caaaatgggg cttccctcta tcctcgcgca gttgctcaag agttggagcg
      601 ggatagccga attgttcaaa tgtatccatt gtagccattt cgctgatttt gcatgtttcc
      661 gccaaggtac cgtcaaaatc gaataatagt aatcccatct tccatcttcc cttcgatcgc
      721 aaatatgaac cggatgcgga caacagaggt gtaacctgct attacgcaag gactcaaaat
      781 tccgaatgtt cactataatc aaaattatag caaacaaaaa cgacagaata atgaggtttt
      841 atttagaatt tggaaagaat tcgctagttc tcactatctc caaccgctta tttccgaacg
      901 ttaggagata gacgatttat caatgtactg attttaccca cccagctatt gcccgttgcg
      961 gtatccatct ttggacaggg gcaattgacg gaatatcaga atttgcgtat gcttttgagt
     1021 aaacccagaa agggagttgg aagcgaaatg gcacagaaaa aagaactgcg acgccaattt
     1081 gaagcggtgc cgattgaatc cggcatctac gaagtacaga atacggaaaa tcaaaagatt
     1141 ttcatcggaa aaaccccgaa tctgaagact ttgaatggca tgaaacacat tttgaatgtg
     1201 ggggcgaaca acaacaagga gctccaatcc gattggagcc acttcgggca ggacgccttc
     1261 atctttacga ttttggaagt gctggaaaag agcgacgacc cttccttcaa tgagaagaag
     1321 gccttggaac aattggaaga aaaatggctg agtgacaagc aacctttcgg agaacgcggc
     1381 tacaaccgca gacccaaagc ctaaaaaaga aaacaagcgc aattgcgcct gttttctttt
     1441 ttagttgatc ccgtaagcat gattgagcgg cccgctgcct ttgcccaaat ccattccatc
     1501 acggagtgcg ccggtgatgt aggctttggc ttggcggatg ctcgcttcca ttccgtgac
//
LOCUS       DAOMUJ010000454         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22165_length_1558_cov_1.493679, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000454 DAOMUJ010000000
VERSION     DAOMUJ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22165_length_1558_cov_1.493679"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLT73_09330"
     CDS             <1..967
                     /locus_tag="PLT73_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRM20611.1"
                     /translation="VETLAPTYRLQIGVPGRSNAFDISKRLGLNPSIIDQARGFIDVD
                     SQNLNEMIADLEQKRRVTEKESLQLQTQLEESDKLLADLKRANEKLETNKEKIIEDAK
                     KEANKLVDTAKEEAEFLLQEIREMQLNMGKSGSIKEHELIDLRRQFNDLRQEESLAKN
                     KVLRKEKEKKMFKPGDEVITETYGQRGTLVEKKGNSEWVVQMGIMKMKLPESDLRLIK
                     EEPQQQKRQRQIATVKSSSSSHVATQLDLRGQRYEEALAEVDQYLDAALLAGYPQVTI
                     VHGKGTGALRKGVGDLLKRHPQVLSYEYAPVNAGGNGATIVTFRG"
     gene            1043..1354
                     /gene="trxA"
                     /locus_tag="PLT73_09335"
     CDS             1043..1354
                     /gene="trxA"
                     /locus_tag="PLT73_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM20612.1"
                     /translation="MVKILTDANFETETKNGVVLVDFWATWCGPCRMQSPIIDELDEE
                     IGDKVTFAKMDVDANPQTPSQFGIMGIPTLLIKKDGQVVEKLVGYHAKEMLEETLEKY
                     I"
BASE COUNT          483 a          329 c          416 g          330 t
ORIGIN      
        1 cgttgagact ttggctccga cctatcgcct gcagatcggc gttcccgggc gaagcaacgc
       61 cttcgatatc tcgaaacgcc tcggcctaaa cccgagtatc atcgatcagg cgcgcggttt
      121 cattgatgtg gacagccaaa acctgaacga gatgatcgcg gatctggaac agaagcgacg
      181 cgtcaccgaa aaagaatcct tgcagctcca aacgcagttg gaagaatccg acaagttatt
      241 ggcggacctg aagcgagcca acgaaaaact ggaaacaaac aaggaaaaaa tcatcgaaga
      301 cgcgaaaaaa gaagccaaca aattggttga tacggcgaaa gaagaggcgg aattcctgtt
      361 gcaggaaatc cgtgagatgc aactgaacat ggggaaatcc ggtagtatca aagagcatga
      421 attgatcgat ctgcgcagac aattcaatga cttgcgccaa gaagagtctt tagccaaaaa
      481 taaagtcctg cgcaaagaga aagagaaaaa aatgttcaag ccgggtgatg aagtcattac
      541 ggaaacatat ggccagcggg ggacgctcgt cgaaaagaaa ggcaattccg aatgggtcgt
      601 ccagatgggc atcatgaaga tgaagttgcc ggaatcggac ctcaggctga tcaaagaaga
      661 gccgcagcaa caaaaaagac aacgtcagat tgccactgtc aaatcatctt catccagtca
      721 tgtcgcgacc cagctcgatc tgagaggcca gcgctatgaa gaagcattgg cggaagtcga
      781 tcagtatttg gatgctgctt tgttagcggg ctatccgcaa gtgactatcg ttcatgggaa
      841 agggacaggt gcactccgca aaggggtcgg cgatttattg aagcgtcacc cacaagtgct
      901 ttcttacgag tatgcaccgg tgaatgctgg gggaaacggt gcgacaatcg taacgttccg
      961 cggctgagca gattgattgg tactgagtat agcctctgct ataattaaca tataaaggtg
     1021 aatttcgaag ggagtaaata caatggtaaa aatattaaca gatgcaaatt ttgaaacaga
     1081 aacaaaaaat ggggtcgtat tggtggactt ttgggcaact tggtgcggac cttgccgcat
     1141 gcaatcacca atcatcgatg aattggatga agaaatcggc gacaaagtaa cattcgctaa
     1201 aatggatgtg gatgctaatc cgcaaacgcc aagccaattc ggcatcatgg gcattccgac
     1261 gttgttgatc aaaaaagatg gccaagtcgt agaaaaatta gtcggctatc acgcaaaaga
     1321 gatgcttgaa gaaacgcttg aaaaatatat ctaaataata agaaagagtt gccggtgtcc
     1381 tgatgtttca ggatatccgg caactctttt tgttttgggt gctgtggtgc gggcgaagtt
     1441 attggcgaaa gctagtggac cggaggagag caccgtttcc ggcggcttat ctccggttgt
     1501 aggccactgg ccggagatga gcccctaaaa tagtacctgc tccgtcgaga atcccttc
//
LOCUS       DAOMUJ010000455         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22175_length_1557_cov_2.153129, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000455 DAOMUJ010000000
VERSION     DAOMUJ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22175_length_1557_cov_2.153129"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLT73_09340"
     CDS             <1..447
                     /locus_tag="PLT73_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HRM20613.1"
                     /translation="EEKRGGRKMDDKKKFEGLKKEQLDENEIRYGQEIREKYGEKTVA
                     ASNNKFAGMSEADFDAMQEMAARLQELLTEAMATGDAGGEAALAVAALHKQWLSYTWP
                     SYSPEAHRGLAQMYVDDERFTAYYDKASGKGASAFLRDAIHNYTDK"
     assembly_gap    631..640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            651..>1557
                     /gene="metG"
                     /locus_tag="PLT73_09345"
     CDS             651..>1557
                     /gene="metG"
                     /locus_tag="PLT73_09345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRM20614.1"
                     /translation="MAKDKNTFYITTPIYYPSGKLHIGNAYSTIACDVMARYKKLMGF
                     DVFYLTGSDEHGQKIENKAAELGITPKEYVDGMAQDMQDLWKRLDISNNKFIRTTDDD
                     HVQAVQQIFEQLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYRDADGNIIGGVAPS
                     GHEVELVKEESYFFRMSKYADRLLAYYDAHPDFIQPESRKNEMVNNFIKPGLEDLAVS
                     RTTFTWGIPVKSNPKHVVYVWIDALANYITALGYGSADDSLYQKYWPADVHMVGKEIV
                     RFHTIYWPIMLMALDLPLPKKIFGHG"
BASE COUNT          439 a          339 c          416 g          353 t
ORIGIN      
        1 gaagaaaaaa gaggaggcag aaaaatggac gacaagaaga aatttgaagg cttgaagaag
       61 gaacagctcg acgagaacga aatccgctac gggcaggaaa tccgcgaaaa atacggagaa
      121 aagaccgtag cggcttcgaa caataagttc gcaggaatga gcgaagcgga ttttgacgca
      181 atgcaggaga tggccgcacg cttgcaggag ctgctgacgg aagcgatggc tacgggggat
      241 gccggcggag aggctgcctt ggcggttgct gcgctgcata aacaatggct gagctatact
      301 tggccgagct actctccgga agcgcatcgt ggactggccc agatgtatgt cgacgacgaa
      361 aggttcacgg catactacga caaagcatca ggcaaagggg cctctgcatt tttgcgggat
      421 gcgatccaca actatacgga taagtgacaa atgctggatg atgctgtaga ggccgggagc
      481 tgtaatttgc tttcggcttt tttgtgcatg gatccggagg tgcaggcgtt ggcaagaggt
      541 tgccgcatgt ttcgaatttc gcccccaaac ccttgacttt tgtggtagaa tggtacggta
      601 agttgcgtac attcgcaaag aacttagaat nnnnnnnnnn gcactcattc gtggctaaag
      661 acaaaaatac attttatatc actactccaa tctattatcc gagcggaaag ctccacatcg
      721 gaaatgcgta ttctactatc gcatgcgatg taatggcgcg ctataaaaaa ctgatgggat
      781 tcgatgtgtt ctacctgacc gggtcagatg agcatggaca aaaaatcgag aacaaagcag
      841 cagaattggg catcacaccg aaagaatatg tggacggtat ggcccaggat atgcaggatc
      901 tatggaaacg tctggacatt tccaacaaca agttcatccg caccactgat gatgatcatg
      961 tccaagccgt tcagcagatt tttgaacaat tattggaaca aggcgacatc tatcttggcg
     1021 aatacgaagg ctggtattct gtttccgacg aagagttctt cacggaaacc caattggctg
     1081 aagtgtaccg cgatgccgac ggaaacatca tcggcggagt tgcgccaagc ggccatgaag
     1141 tcgaattggt gaaagaggag tcctacttct tccgcatgag caaatacgcg gatcgtcttt
     1201 tggcgtatta cgatgcgcac cctgatttta tccaaccgga atcgcgcaaa aacgagatgg
     1261 tcaacaattt catcaaacca ggacttgagg atttggctgt atcacggacg acctttactt
     1321 gggggattcc tgttaaaagc aatccgaaac acgtggtcta tgtttggatc gatgcattgg
     1381 cgaactacat cactgcctta ggttatggtt cggctgatga ctcgctttac caaaaatatt
     1441 ggccggctga tgtgcacatg gtcggaaaag aaatcgtccg cttccacacc atctattggc
     1501 cgatcatgct gatggcactg gatctgccgc ttccgaaaaa aatctttgga cacgggt
//
LOCUS       DAOMUJ010000456         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22230_length_1554_cov_1.667111, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000456 DAOMUJ010000000
VERSION     DAOMUJ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22230_length_1554_cov_1.667111"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="PLT73_09350"
     CDS             <1..104
                     /locus_tag="PLT73_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM20615.1"
                     /translation="LFLMVTTYLVFLLPISLLIHFIERRLRFAGFGN"
     gene            85..>426
                     /locus_tag="PLT73_09355"
     CDS             85..>426
                     /locus_tag="PLT73_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM20616.1"
                     /translation="MQDSGINILFEGQNLQRILEGLGQTVFVAIISVVLSFILGLLVG
                     MLMTSRNIVVRFVTRLYLEVVRIVPVLVMLYILYFGLTRWTGIHIDNRVIAIFVFTFW
                     GTAEMADIVRSS"
     assembly_gap    427..436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <437..756
                     /locus_tag="PLT73_09360"
     CDS             <437..756
                     /locus_tag="PLT73_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM20617.1"
                     /translation="IQWESGQALGLTAGQLYVYVIIPQTVRRIVPPAINLATRMVKTT
                     ALLPLIGIIEVIKVGQQVIEVSVKDYPSASFWIYGLIFFLYFIVCYPLSKLSKKLELK
                     WQE"
     gene            774..1517
                     /locus_tag="PLT73_09365"
     CDS             774..1517
                     /locus_tag="PLT73_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM20618.1"
                     /translation="MESPILKLKHVNKYYGETQALADIQLDVAKGEVVTIIGQSGCGK
                     STALRCINGLEQIQSGSILLDGKEIAAAGTNWKEVRQKIGMVFQKYDLFPHMTVLDNV
                     LLAPLKVQKRKKEEAAEEAIALLKRVGLEDKAHSFPRQLSGGQQQRVAIVRALMTHPE
                     IMLFDEVTAALDPEMIREVLNVILDLAKDGMTMLIVTHEMAFAEAVSDKIVFMEKGNI
                     VEISEDPKAFFTQPQSQRANEFINTFRFQ"
BASE COUNT          430 a          317 c          390 g          407 t
ORIGIN      
        1 cgctattctt gatggtcaca acctatctgg tgtttttatt gccgatatcc cttttgatcc
       61 atttcatcga aaggaggttg cgttttgcag gattcgggaa ttaacatttt attcgaaggt
      121 caaaatctgc aacgtatctt ggaaggcctt gggcaaacgg tttttgtcgc aatcatatca
      181 gtcgtgcttt cattcatact gggacttttg gtcggaatgc tcatgacaag cagaaatatc
      241 gttgttcggt tcgtcacaag gctctacctc gaagtcgtac ggattgtccc cgtattggtg
      301 atgctgtata tcctgtactt cggccttaca cgttggacag gcattcatat tgataatcga
      361 gtgatcgcca ttttcgtgtt taccttttgg ggtactgctg aaatggccga catcgtcaga
      421 tcatccnnnn nnnnnnaaat ccaatgggaa agcggacagg cattaggatt gactgcaggg
      481 caactctatg tgtatgtcat tattcctcag accgtccggc gcatcgttcc accggccatc
      541 aacttggcta ccaggatggt gaaaacgaca gcgctgttgc cgttgatagg gattattgaa
      601 gtcatcaagg tcggacagca agtgattgaa gtatccgtga aggactatcc gagtgcttcc
      661 ttctggatct atgggttgat cttcttcctg tacttcatcg tctgttaccc gttatcgaaa
      721 ttatccaaaa aattggaatt gaaatggcaa gagtgagaag gggtgaagaa acattggaaa
      781 gtccaatatt aaagctgaaa catgtaaata aatattacgg agaaactcag gccttggctg
      841 atatccagtt ggatgtagcc aaaggagaag ttgtgaccat catcggacaa tcaggttgcg
      901 gcaaatcgac tgccctgcgc tgcatcaatg gtctggagca aattcagtcc ggtagcatct
      961 tgctggatgg aaaagagata gccgcagcag ggacgaattg gaaagaagtc aggcagaaaa
     1021 tcggaatggt ctttcaaaag tatgatctgt ttccgcacat gacagttctt gataatgtcc
     1081 tattggctcc tttaaaagtt caaaaacgca agaaagagga ggcagccgaa gaagccattg
     1141 cgttgctgaa acgggttggt ctggaagata aagcgcatag cttcccgagg cagttgtccg
     1201 gcgggcagca gcaacgggtt gcaatcgtgc gcgcattgat gacgcacccg gaaatcatgc
     1261 tcttcgatga agtgacggcg gcgctcgatc ccgagatgat cagggaagtc ttgaacgtca
     1321 tcctcgatct ggcaaaagac ggcatgacga tgctgatcgt cacccatgaa atggcttttg
     1381 cggaggcagt cagcgataaa atcgtcttta tggaaaaagg gaacattgtt gaaatttctg
     1441 aggatccgaa agcttttttc acgcagccac aatcgcaaag ggctaacgaa ttcatcaaca
     1501 cattcaggtt tcaatagtaa gacaatatag ataaaaaaac attggaggaa tgaa
//
LOCUS       DAOMUJ010000457         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22425_length_1541_cov_2.716016, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000457 DAOMUJ010000000
VERSION     DAOMUJ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22425_length_1541_cov_2.716016"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PLT73_09370"
     CDS             complement(<1..1011)
                     /locus_tag="PLT73_09370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRM20619.1"
                     /translation="MSFFIYFTLLFGATILASTLFTYLFRAAARRFRWMDKPSEAKVH
                     IKETPTMGGVAIFAAYWGAFFLGVPLSNRSGFAEAMFLSSLIILVTGIIDDTYDLRPW
                     QKMIGILTAANVLYFFSDIKIDSLTLDYFGTIEFHEAGYLVMMLWIVVITNAVNLMDG
                     LDGLATGTSIISLSTMGFVSYFFTSHMGITAVVMIFLLVASLLGFLPFNFYPSSIFLG
                     DTGSLFVGFMIAVLSLFGLKHATFVSLLIPVAILGVPLTDTIAAVLRRTLHKRSISAK
                     DKSHLHHRLLRLGFTHRQTVLVIYALAIIFSLTAILFPISSFWGTMVLVVGLIYGVVL
                     FIA"
     gene            complement(1086..>1541)
                     /locus_tag="PLT73_09375"
     CDS             complement(1086..>1541)
                     /locus_tag="PLT73_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM20620.1"
                     /translation="LAELLATLQEWDEPTIVVFWGDHWPSVFGEELYALNTLQNMHET
                     PLFIYSNTQEVHKDLGVTSPIYFLPEVLELSSSRVTAFEALLMALQEQVPAFEKALYV
                     NGNTGEYVSSREGLSEQARSLLADYDLIQYDTTTGNRYAEANGFYRIAD"
BASE COUNT          465 a          414 c          341 g          321 t
ORIGIN      
        1 ggcaatgaag agcaccactc catagatcaa accgactacc aaaaccatcg ttccccagaa
       61 agaagaaatc gggaacagga tagcggtcaa agaaaagatg atcgccaaag catagatgac
      121 caaaacggtc tggcggtgtg tgaaccccaa ccgcaacaac cggtgatgca gatggctttt
      181 atccttcgcg ctgatggaac gtttatgcaa agtccggcga aggacggcag cgatcgtgtc
      241 cgtcaaggga acgccgagaa tggccaccgg tatcagcagc gatacgaagg tggcatgttt
      301 caaaccgaac aaggacagca ctgcgatcat aaagcctacg aacaaagatc ccgtatcccc
      361 cagaaaaatc gaagacggat agaagttgaa gggcaaaaag cccaacaaac tcgcgaccaa
      421 aaggaagatc atgacaacag cggttatgcc catatgcgag gtgaaaaaat agctgacgaa
      481 acccatcgtc gagagggaaa tgatcgaagt ccccgtcgcc aaaccatcca gcccgtccat
      541 cagattcacc gcattcgtga tgaccacaat ccaaagcatc atcaccaagt aacccgcttc
      601 atggaattcg attgttccga aataatccaa agtcaggctg tctattttaa tgtctgaaaa
      661 aaaatagagg acattcgccg cagtgaggat gccgatcatt ttttgccagg gcctcagatc
      721 ataagtatca tcgatgatac ccgtcacaag aatgatcagc gagctcaaaa acatggcttc
      781 cgcaaaaccg gaacgattac tcagaggaac cccaaggaaa aacgcgcccc aataagctgc
      841 aaatatagcc acgcctccca tagtcggcgt ttcctttata tgcactttcg cttcggaagg
      901 tttatccatc cagcggaacc gcctcgctgc cgcacggaac agataggtga acaacgtact
      961 cgccagaatc gtcgcaccga aaagaagcgt aaaatatata aaaaaactca ccggatccct
     1021 cctttcatcc tatatttcct taatcatagg cgattccggt tctacatgca aatcggccat
     1081 ctttttcaat cggcgattcg ataaaaacca tttgcttccg cgtatcgatt tccagtggtc
     1141 gtgtcgtact ggatgaggtc ataatccgcc aacaggcttc ttgcctgctc ggacagtccc
     1201 tctcggctcg agacatattc cccggtgttg ccattcacat aaagcgcctt ttcaaaagct
     1261 ggcacctgtt cctgtagcgc catcaacagg gcttcaaaag cagtaacacg gctgctgctc
     1321 aattccagca cttccggcaa gaaatagata ggactggtga cccccagatc cttatggact
     1381 tcttgcgtat tgctgtagat gaacagaggc gtctcatgca tattttgaag tgtattcagc
     1441 gcgtaaagtt cctcgccgaa aacgcttggc caatgatccc cccaaaaaac aacgatggtt
     1501 ggctcatccc actcctggag ggtagccagc aattccgcca a
//
LOCUS       DAOMUJ010000458         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22550_length_1536_cov_1.429440, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000458 DAOMUJ010000000
VERSION     DAOMUJ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22550_length_1536_cov_1.429440"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLT73_09380"
     CDS             complement(<1..186)
                     /locus_tag="PLT73_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRM20621.1"
                     /translation="MFTKVLIANRGEIAVRIIRACHELGIQTVAVYSEADREALHTQL
                     ATQAICIGPAKASDSYLN"
     gene            complement(188..599)
                     /gene="fabZ"
                     /locus_tag="PLT73_09385"
                     /pseudo
     CDS             complement(188..599)
                     /gene="fabZ"
                     /locus_tag="PLT73_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053359.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
     gene            complement(618..1079)
                     /gene="accB"
                     /locus_tag="PLT73_09390"
     CDS             complement(618..1079)
                     /gene="accB"
                     /locus_tag="PLT73_09390"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRM20622.1"
                     /translation="MNIEDVKSLISLIDDSTLTELHYQTGDFQLLLSKNKLVQTNQQS
                     SDNIQAEVPSPAVIAPVQNLKEAVSEAATAKEGKIITSPIVGVVYLAPTPEAKPYAIK
                     GQSIAKGDVVCLVEAMKLMNEIKSEFDGEIVEVLVENEQIVEYNQPLFRIV"
     gene            complement(1083..>1536)
                     /locus_tag="PLT73_09395"
     CDS             complement(1083..>1536)
                     /locus_tag="PLT73_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRM20623.1"
                     /translation="CDAYHMTAPSADGSGAGKAMILAMKEAGITPAQVDYINAHGTST
                     PANDSGETAAIKYAFGEEAHNVAISSTKSMTGHLLGAAGGIEAVACVKAIQDDFMPPT
                     IGFQTADENCDLDYIPNVGREKEVTYTLSNSLGFGGHNAVLCFKKWEA"
BASE COUNT          380 a          361 c          332 g          463 t
ORIGIN      
        1 attcagatag gaatcgctag cttttgcagg gccgatacag atagcctgag tggctagttg
       61 tgtatgcaat gcttcgcggt cagcttcaga atagaccgca accgtttgga ttcctaactc
      121 atgacaagca cgaatgatcc tgacagcaat ttctcctctg tttgcgatca ataccttcgt
      181 aaacatatta tctaccaatg atgaatgtca tttcaacttc acaaagcttg cccataaccg
      241 acacctgcaa atttcttcaa tttgacgata tccacttgga ggcgaagaac gtctcctgga
      301 acaacttttt gacggaattt gactttattc aatccgccca agtaagctgt ctgacccttg
      361 aaatcaggca atgtcaacaa cggaatagat cctgcttgag ccaatgcttc aacaatcagt
      421 acacctggca ttactggctc tccagggaag tggccttgga agaaaggctc attgattgtt
      481 acgtttttgt agcagacgat gtgttcgcct ggtgtcaatt cttcaacttt atcgatgaag
      541 aaaatcggat agcgattcgg gatgatatcc attacttctt gtgcactcaa taaactcatt
      601 ttattagtct ccttttatta gacgatacgg aacaatggtt ggttgtattc aacgatttgt
      661 tcattttcaa ccaacacttc aacgatttcc ccatcgaatt cgcttttgat ttcattcata
      721 agtttcatcg cttccaccaa acagacaacg tcgccttttg cgatgctctg gcctttaatg
      781 gcgtaaggtt ttgcttcagg ggtcggtgcc aagtaaacaa caccgacgat cggggaagtg
      841 atgatttttc cttcttttgc agtagccgct tcagaaacag cctccttgag gttttggact
      901 ggagcgatca ctgctggact aggaacttct gcctggatgt tgtcgcttga ctgctggttc
      961 gtttgaacca acttgttctt cgacaacagg agctgaaagt cacctgtctg atagtgcaac
     1021 tcagtcaagg ttgaatcatc gatcaaggag atcaggcttt ttacatcttc aatattcatg
     1081 tattaagcct cccatttttt gaaacatagt acggcattgt ggccgccgaa gcccagagag
     1141 ttactcaatg tgtaagtgac ttctttttca cgtcctacat tcgggatata gtctagatcg
     1201 caattttcat cagccgtttg gaagccgatt gtaggcggca tgaaatcatc ttggatagct
     1261 ttgacacagg caaccgcttc gataccgccg gctgcgccca gcaaatgtcc cgtcatactc
     1321 tttgtgcttg agatagcgac attgtgagct tcttcgccga atgcgtactt gattgcagcc
     1381 gtttcgcctg aatcatttgc aggcgtactt gttccatgag cgttgatgta atcgacttgc
     1441 gctggagtga tgcctgcttc tttcatcgct aggatcatcg ctttgccagc accgcttcca
     1501 tcagctgatg gagccgtcat atgataggca tcacag
//
LOCUS       DAOMUJ010000459         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22563_length_1535_cov_2.171622, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000459 DAOMUJ010000000
VERSION     DAOMUJ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22563_length_1535_cov_2.171622"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLT73_09400"
     CDS             <1..534
                     /locus_tag="PLT73_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM20624.1"
                     /translation="GIIAEGNLIHGTAGSGGEIGHMTVEPGGFDCTCGKKGCLETVAS
                     ATGVVKLARKHAEEYAGNAQLKFIIDDGQEITSKMIFDLAKEGDELAVLVVDRAAYYL
                     GLACSHIGNLLNPAYIVIGGGVSAAGEYLLEQVRTYFADFTFPNVKKTTHIKLAALGN
                     DAGIVGACYLALMETSK"
     gene            778..>1535
                     /locus_tag="PLT73_09405"
     CDS             778..>1535
                     /locus_tag="PLT73_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20625.1"
                     /translation="MDDTTILLALESNGEYGLYSHDLDTGKEQPIRTFSDRLVFVALS
                     ANGRRILVQVANQQGNNVMMIDKSGNALAEIRFSEGLLTGVSWNPANEGKLFLSAQKT
                     VGSLENYLWDLSSDEAMVALPVTGEMPVWYSENMYLQKKTANDGVSALVLSDVRLPGK
                     DTVIDQEILAYGLEDESVWVVTPSDFNAEELFVVSYYPLLIAQGYVALPRIADGSRLI
                     IPEFETGRDSDEIFALLPKQESDIAAGITFELAK"
BASE COUNT          402 a          334 c          409 g          390 t
ORIGIN      
        1 ggaatcatag cggaaggcaa tctgatccat ggtactgcag gttccggcgg cgaaatcggc
       61 cacatgactg tagaaccagg cggatttgac tgtacgtgcg gaaagaaagg ctgtttggag
      121 accgtcgcca gcgcaacagg cgtcgtcaaa ttggcccgca agcatgctga agaatatgcc
      181 ggcaacgcgc agctgaaatt catcatcgac gatggccagg agattacttc caaaatgatt
      241 tttgatttgg caaaagaggg agacgagttg gctgtactag tagtcgatcg cgctgcctat
      301 tatctgggtt tggcctgcag ccatatcggc aatcttttga atccggctta tatcgtcatc
      361 ggtggcggtg tatcagcagc cggggaatac cttttggagc aagtaagaac ctatttcgct
      421 gacttcacat tcccgaacgt caagaaaacg acgcatataa aattagccgc gcttgggaat
      481 gacgcaggga tagttggggc atgttacctc gcactgatgg aaaccagcaa ataaatcaat
      541 ccaggcagaa aatctctgga aatttttcgg ataggaaaat gcgggaggat ggggaatggc
      601 atgataaaaa ggcgactagc accaatcatt ctctcgctat caagtattct tattctgact
      661 gcctgtgcac agcgactatc gtatcctgag caatccgggg taagtgaaca aagcctgtct
      721 tccggccccg acatccgatc gattcctaag gatgaattcc tatgcacggt aggttggttg
      781 gacgatacga ctattcttct tgcattggaa tcaaatggag aatacggtct gtacagtcat
      841 gacctggaca caggtaaaga acagccgatc cgaacttttt cagatcggct tgtttttgtc
      901 gcgctttccg caaatggtcg gcgaattctg gtgcaagtcg caaatcaaca gggaaataat
      961 gttatgatga tcgacaagtc aggtaatgcc cttgccgaaa ttcgtttttc cgagggactg
     1021 ttgacgggtg tttcctggaa tccggcaaac gaaggaaagt tgtttctgtc cgctcaaaaa
     1081 acagtgggca gtttggaaaa ttatctgtgg gatttatctt ccgatgaggc aatggtggca
     1141 ctgccggtca ctggggaaat gccggtttgg tattcggaaa atatgtacct ccaaaagaaa
     1201 accgcaaatg atggcgtctc tgcattggtg ttgagcgacg ttcgtttacc cggcaaagat
     1261 actgtcatcg atcaggaaat ccttgcttac ggtttggaag acgaatccgt atgggtagtg
     1321 acaccttccg atttcaatgc ggaagagttg tttgtggttt cttactatcc gcttctgatt
     1381 gctcaaggtt atgttgcctt gccgcgcatc gcagatggga gcaggctgat catcccggaa
     1441 ttcgaaacag ggcgtgactc ggatgagata tttgctttgc ttccgaaaca ggagtcggac
     1501 atcgctgccg gcatcacctt tgaactcgca aaaat
//
LOCUS       DAOMUJ010000460         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22686_length_1529_cov_1.782904, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000460 DAOMUJ010000000
VERSION     DAOMUJ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22686_length_1529_cov_1.782904"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1358
                     /gene="hflX"
                     /locus_tag="PLT73_09410"
     CDS             99..1358
                     /gene="hflX"
                     /locus_tag="PLT73_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRM20626.1"
                     /translation="MENTKEKVILVNVQTNQTDEAFEYELRELAELTETALGEVVGVL
                     TQKRERKDSRTVIGKGKVEELVNLCAELEADTVIFQQELSSKQVRNIQETVDCKVIDR
                     VQLILDIFAMRARSKEGKLQVALAQLSYLLPRLMGQGVNLSRLGGGIGTRGPGETKLE
                     TDRRHIHSQMQEIKKTLEETELHRQRAREKRHASEVFQIGLIGYTNAGKSTIINRLTD
                     AATLEEDKLFATLDPLTRKLTLPNQFQATITDTVGFIQDLPTQLIHAFHSTLEESRNM
                     DVFLHVVDASTSFIDQHEKTVLALLKDLDMDKTPMLTIYNKRDLASPDFQPRLFPNIV
                     ISALEDDDINRLKDFIWDEIAKMLVPYTKDIDASGPEARTMNKMQQETFVESIEYIEE
                     TNAYRLRGYAKTNSKWLGDKVTDASDE"
     gene            1342..>1529
                     /locus_tag="PLT73_09415"
     CDS             1342..>1529
                     /locus_tag="PLT73_09415"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRM20627.1"
                     /translation="MQVTNKEELLADLVVKVDNKIQPVFKEIQEKSLFNQGKVLEAFR
                     KHRVSDYHFNPSTGYGYD"
BASE COUNT          493 a          299 c          380 g          357 t
ORIGIN      
        1 gttcggaaca gccatgatat gcagaaaatc gaagatttag tcgaggcgtt cttagcctga
       61 tggatgataa agaacgtata aagaaagttg gaaattctat ggaaaatacg aaagaaaaag
      121 taattctggt taacgttcag acgaatcaaa cagatgaagc ctttgaatat gagctgagag
      181 aactggctga attgaccgaa accgcattag gggaagttgt cggcgtattg acccaaaaga
      241 gggaacgtaa ggattcgcgc actgtaatcg gcaaagggaa agtggaagag ctagtgaatt
      301 tgtgtgcaga attagaagca gatacggtca ttttccaaca ggagctttct tccaagcaag
      361 tgcgcaacat ccaagaaact gtcgactgca aagtcatcga tcgtgttcaa ctgatattgg
      421 atatctttgc aatgcgcgca cgcagtaaag aagggaaact tcaggttgcc ttggcgcagt
      481 tgagttatct attgccgcgt ttgatgggtc aaggcgttaa tttatcccgt ctaggcggtg
      541 gaatcggaac aagaggaccg ggtgagacaa aactcgagac cgaccgtaga cacatacaca
      601 gtcagatgca ggaaatcaaa aagacattgg aagaaacaga acttcaccgt caaagggcaa
      661 gagaaaagcg acatgccagc gaagttttcc aaatcggctt gattggttac acgaatgcag
      721 gcaaatcgac gatcattaac cgcttgactg atgcagcaac gttggaagaa gataaattgt
      781 ttgcaacgct ggatcctttg acaaggaagc tgacattgcc taatcaattc caggcaacta
      841 taaccgacac tgtaggtttt atccaggacc ttccgacaca gttgatccat gctttccatt
      901 caacgttgga ggagagccgc aatatggatg tatttttgca tgtagtggat gcttcgacct
      961 ccttcatcga tcagcatgag aagaccgtct tggcattgtt gaaggatttg gacatggata
     1021 agacacctat gttgacgatc tacaacaaac gggatcttgc aagcccggat ttccagccga
     1081 gactcttccc gaacatcgta atttctgccc ttgaagatga cgacatcaac cgattgaagg
     1141 acttcatctg ggatgaaatc gccaaaatgt tggttccgta tacaaaggac attgatgcaa
     1201 gcggccctga agcccggacg atgaataaga tgcagcaaga gacctttgtg gaatccattg
     1261 agtacataga ggaaacaaac gcatatcgct taagaggata tgctaaaacg aattcaaagt
     1321 ggttaggaga caaagtaact gatgcaagtg acgaataaag aagaactact ggcggatctg
     1381 gtcgttaaag tggacaacaa aatccagccc gtgtttaaag aaatacagga gaagtcttta
     1441 tttaatcaag gcaaggtgct tgaagctttc cggaagcacc gcgtcagcga ttaccatttt
     1501 aatcctagta caggctatgg ctatgatga
//
LOCUS       DAOMUJ010000461         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22732_length_1526_cov_2.390891, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000461 DAOMUJ010000000
VERSION     DAOMUJ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22732_length_1526_cov_2.390891"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..>1526
                     /locus_tag="PLT73_09420"
     CDS             516..>1526
                     /locus_tag="PLT73_09420"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM20628.1"
                     /translation="MTTQTTLSRTKAPFRADHVGSFLRPESIKKARKELAEGKITKEA
                     LREIENVEITRIVDKQIALGYKGITDGEFRRSYWHFDFLENLLGFEGYLAAQGKQFHN
                     VVTSAHSVRNIGKIAFNPEHPFFADYAFLAEAVGDRAVAKVSIPSPNQLIRLGFRNEE
                     IYPTLEEYAADLQQAYRDTILHFYSLGNRYIQIDDTFWGDLCSDKALEIFGSQETYDE
                     LKRVATENVKNIQIGLPEDLVVTTHICRGNFRSSYSQEGAYEPVAKELFGETGFDGFF
                     LEYDTDRAGGFEPLRYYTGDGQIILGLITSKTPELEDIAEVKARITEATKYVPLEQLC
                     LSP"
BASE COUNT          454 a          363 c          346 g          363 t
ORIGIN      
        1 tcagccttga gcccttcaca tagacatcta tctatatgaa gtatataccc tcacggtatg
       61 aattgcaatt tccttcttga acagaaagaa tcgaaatgaa aatacaccaa aatgaaaaat
      121 taattaaaaa aggtttgact cattcttatt cgcttgttat tatatatata taaatttcaa
      181 aataaaatca cattgaaaag ataagtacgg atggacgaaa tgcacagaga acctcggcag
      241 ttgagaagag gacagggatg ctatccggaa aatggtcttc gagtgagtgg acggcgaacg
      301 ctctttttag aaccttagct ctccacggga atgcccgtta cagcatcaca gtatcccgga
      361 acagcgattt gttgcggcgt actggttaag gggaaaatcg agaggttttt ctgaatcaag
      421 gtggtaacac ggtgaatttt tcatacgtcc ttgcgcaaca tattgcgcaa ggacgtattt
      481 ttttacaaaa aaacaaacga aagaaggaaa atatcatgac aacccaaaca actttaagca
      541 gaacgaaggc gccattccgc gcagaccacg taggaagttt cttgcgtccc gaaagcatca
      601 aaaaagcccg caaagagctg gctgaaggca aaatcacgaa ggaagccctg cgcgaaatcg
      661 aaaatgttga aatcacgcgc atcgtcgaca aacagattgc gcttggctac aaaggcatca
      721 ccgacggaga gttccgacgc tcttattggc atttcgattt ccttgagaac ctcttgggct
      781 ttgaaggtta ccttgcggct caaggcaaac aattccacaa cgttgtgacc agcgcccaca
      841 gcgtccgcaa catcggcaaa atcgccttta atccggaaca tccattcttt gcggactatg
      901 ctttcttggc tgaagcagtc ggcgatagag ccgttgcgaa agtatcgatc ccgagcccga
      961 accaattgat ccgcctcggt ttccgcaatg aagaaattta tcctacattg gaggagtatg
     1021 ctgccgatct acaacaggca taccgcgaca ccatcttgca cttctactcg ttgggtaacc
     1081 gttacatcca gatcgatgac actttctggg gcgacctttg ctcggacaaa gcattggaaa
     1141 tcttcggttc tcaggaaact tacgatgaat tgaaacgcgt cgctactgaa aacgtcaaaa
     1201 atatccagat cggtctgcct gaagatctgg tcgtcacgac gcatatctgc cgcggcaatt
     1261 tccgctcgtc ctactcgcaa gaaggcgctt atgagcctgt tgcgaaagaa ttgttcggcg
     1321 aaaccggctt tgacggcttc tttctggaat acgacactga ccgtgctggt gggttcgaac
     1381 cattgcgcta ctacacaggc gacggccaaa ttatcctagg cttgatcact tcaaaaacac
     1441 cggaattgga agatatcgcc gaagtcaaag cgcgcatcac tgaagcaacc aagtatgtgc
     1501 cgttggagca attatgcctg agcccg
//
LOCUS       DAOMUJ010000462         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22770_length_1524_cov_2.021784, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000462 DAOMUJ010000000
VERSION     DAOMUJ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22770_length_1524_cov_2.021784"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLT73_09425"
     CDS             <1..1164
                     /locus_tag="PLT73_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM20629.1"
                     /translation="QAGMDYNSVVIATILTTAVSNILMGLYANSPLILAPGMSDNAFF
                     TYILVDAFAFTWQQSLSIIFIVGLLFFLLTHFNVVDQLLRSIPVTLIKGMSAGVGFFL
                     IFLGLKNGGIIVSSEGTIVALNNIFQPVPLTLLATLLVGLILFVKNVKGNFLLTIIFG
                     VLISIALGVTDISSFSYSFIDLGSLEPFVFQLDFSGVLSMDYWVAVFSLLILVVFQNL
                     GTQVSFLTSEQPKVLKRTLESNALSVMIAGLLGCSSTCTSAEGATGIAVGGRSGLTSF
                     MVGVYFLFTIALIPFIALIPLAVISALLIIVGSLLAANNLKGINFDDFTEYFPAILMV
                     LMMVLTFNIADGIGWGFILYTFIKVFSGDRQAVSKMMYGLTGIFILYFVLKFI"
BASE COUNT          338 a          323 c          378 g          485 t
ORIGIN      
        1 caagccggca tggattataa ttcggttgtg attgcgacga tactgacaac ggccgtcagt
       61 aacattctga tgggcctgta tgccaattca cccttgatac ttgccccagg gatgagcgac
      121 aatgctttct ttacctatat cttggtcgat gcctttgctt ttacttggca acaaagttta
      181 tcgattatct ttatcgtcgg gctactcttc tttctgttga cgcatttcaa cgtggtagat
      241 caactccttc gctccatccc ggtgactttg atcaaaggga tgagcgcggg agtcggcttc
      301 ttcctgatat tccttggttt gaagaacggc ggcatcatcg tatcctcaga gggtacaatc
      361 gtggcactga ataatatttt tcaaccggtg ccgttgacgc tcttggcaac gctgctggtc
      421 gggctgatcc tgttcgtcaa aaatgtaaaa ggcaattttt tattaacgat tattttcggt
      481 gttctcattt ccattgcgct aggggtaacg gatatcagct ccttttccta ttccttcatc
      541 gatctgggtt ccttggagcc gtttgtcttc caacttgatt tttcgggcgt tctgtcgatg
      601 gactactggg ttgccgtatt ttctcttctg attttggtcg ttttccagaa cttagggaca
      661 caagtcagtt ttttgacatc cgaacaaccg aaagtattga aacgaacgct ggaatcgaat
      721 gctttatcag tgatgattgc cggtttgttg ggctgcagct ccacctgcac aagtgcggaa
      781 ggcgcaacgg gcatcgctgt aggaggaaga agcggattga catcctttat ggtgggcgtt
      841 tatttcctgt tcaccatcgc gttgattccc ttcattgccc ttatcccgct ggcggtcatc
      901 tcggctttgc tcatcattgt agggtccttg ttagcggcca acaatctgaa gggcatcaat
      961 tttgatgatt tcacggaata ttttccggct atcctgatgg tgctgatgat ggtgttgacg
     1021 tttaatatcg cggatggtat cggctggggg tttatcctgt atacgttcat caaagtgttt
     1081 tcgggtgatc gacaagcggt atcaaaaatg atgtacggat tgacgggtat ctttattctg
     1141 tatttcgttt tgaaatttat ttgaaaaagg caaaaagctg gtctcttttg gagatcggct
     1201 ttttgctttt tgatcgagac gcaggggtgc gggtcttagc ttatgcggag tgaatcgctg
     1261 tgctcaccga ttcatcaccg ataaaaaact gtacaggagg gtgagattgt cgaatagtgg
     1321 acggctattc gactaattaa cctcagcgta aggtgaaatt gtcgaatggg tgaaatcatt
     1381 cgacaatttg atcacagcat ggaacggaat tgtcggatgt tgcagtgaca ttcgacaatt
     1441 ctcccccaac gtaagacgaa atccgcgaat gttggaggtc tattcgggga accggccgcg
     1501 ccacacttta atatttttat ttcg
//
LOCUS       DAOMUJ010000463         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22787_length_1523_cov_2.409401, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000463 DAOMUJ010000000
VERSION     DAOMUJ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22787_length_1523_cov_2.409401"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLT73_09430"
     CDS             <1..155
                     /locus_tag="PLT73_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRM20630.1"
                     /translation="IDTTKHVVITSPHPSPLSSYRGFFGSKPFSKTNEALKSFGVEPI
                     DWRLPE"
     gene            192..887
                     /locus_tag="PLT73_09435"
     CDS             192..887
                     /locus_tag="PLT73_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM20631.1"
                     /translation="MKNGKAKLFYQVGGSGDALLLLHGNGESSDIFAQQFPFFTKYFT
                     VYALDTRGHGRSELGVKRLTFKQIAQDILALLDKEGIQRIHVLGFSDGGNLGLYLAAH
                     HPERIASLIAMGANYEADGLTDDCYEETLGRYEELLALPDSDPEKTQLLCVHKLMLEE
                     LDLSAADLSRIQAPTLLLAGEYDLIRDDQTEAMHRLIPGSQKYIVPGGSHSFFVDDPK
                     VLEQLAKKFYSNL"
     gene            1129..1494
                     /gene="nrdI"
                     /locus_tag="PLT73_09440"
     CDS             1129..1494
                     /gene="nrdI"
                     /locus_tag="PLT73_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HRM20632.1"
                     /translation="MKVVFMSLTGQTRKFVNKLGFETMELTAGNPFQKMEEPFIIVTP
                     TYDKEVTDILTDFIETDQNRAYFKGVAGGGNLNFGKLFGFTAKDLAHDYGVPLLHLFE
                     FQGNEKDIQILKEKVTELG"
BASE COUNT          425 a          319 c          391 g          388 t
ORIGIN      
        1 tcatcgacac aaccaagcat gtcgtgatca catcgccgca tccaagtccg ttatcatcct
       61 atagaggttt cttcggatcg aagccttttt caaaaacgaa cgaagcactc aaatctttcg
      121 gcgtggagcc gatcgactgg cgcttgccgg aataaaaaaa gaaaggaatg tcgcctatga
      181 gaggaaagac aatgaagaac ggaaaagcaa aactcttcta tcaggtaggg ggttccggtg
      241 atgccttgct gctgttgcat ggcaacggag agagctcaga catcttcgct cagcagtttc
      301 ctttttttac gaaatacttc acagtctatg cactcgatac acgcggtcat ggccgatcag
      361 agcttggcgt gaagcgcttg accttcaagc agattgcaca ggatatcctg gcgttgctcg
      421 acaaggaagg gattcaacgg atccatgtgc tcgggttcag tgatggcggc aatttgggtt
      481 tatatttagc ggcccatcat ccagaacgga tcgcttcttt gattgctatg ggtgctaatt
      541 atgaagcgga tggcctgacc gatgattgct atgaagaaac cttaggacgc tacgaggaac
      601 tgttggcttt gccggattcc gacccggaaa aaacgcagct actgtgcgtc cataagctga
      661 tgctggagga attggatctt tctgccgcag acctgagccg tattcaagca ccaaccttgc
      721 tgctggcggg tgaatacgat ctgatccgcg acgaccaaac agaggcgatg caccgcttga
      781 ttcccgggtc acagaaatac atcgtgcctg gaggcagtca tagctttttc gtggatgatc
      841 cgaaagtgct ggaacagctc gcgaaaaaat tctattcaaa tttgtgaatt ttggagcgta
      901 tcacgaggtg cacccggaga attatgttag aatgaaactc tgaaatgtcg ccggtggcaa
      961 ccattcagtg cggtgccagt tttggccaat tatagtgaaa atacaaacaa aggcacgata
     1021 tgttgtgtga tttccgtgtg cgcacactac atgttgtgtc tttttgttgt aattcgcttc
     1081 ttacaaacgt ataatggatg aagaacgttt ggaaaagggt gatgcaaaat gaaagtagta
     1141 tttatgtcgt tgaccggcca aacaaggaag ttcgtgaata aactgggctt tgaaacgatg
     1201 gaattaacag ccggtaatcc gttccaaaaa atggaggagc cattcatcat cgttaccccg
     1261 acctatgata aagaagtgac ggatattttg accgatttca ttgagacgga tcaaaaccgt
     1321 gcttacttca agggtgtggc aggtggtgga aacctgaatt tcggcaagct tttcggcttt
     1381 accgcgaaag atttggcaca cgattatggc gtcccgctgc tgcacttgtt tgagtttcaa
     1441 ggcaatgaga aagatattca gattttgaaa gagaaggtga cggaacttgg atagtccaaa
     1501 attagtaacc aaaacagatg atg
//
LOCUS       DAOMUJ010000464         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22869_length_1519_cov_1.804645, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000464 DAOMUJ010000000
VERSION     DAOMUJ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22869_length_1519_cov_1.804645"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>264)
                     /locus_tag="PLT73_09445"
     CDS             complement(<1..>264)
                     /locus_tag="PLT73_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM20633.1"
                     /translation="KQGGMVGAPFGIVGIETAFQLMYSNFVLGGVFTLKQLVDWMTVK
                     PAALFGMGSGTLDIGSQADIAIFDLANEYEIRSEDFLSKATNTP"
     assembly_gap    265..274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<275..1207)
                     /locus_tag="PLT73_09450"
     CDS             complement(<275..1207)
                     /locus_tag="PLT73_09450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRM20634.1"
                     /translation="MSVWIKNAKMLGQKEELIPVEIYVKDDKIAAIGQDLQGTAEDAT
                     VIDAKGGLVTPGLIDVHVHLREPGFTYKETIETGTQAAARGGFTTVCAMPNLNPVPDT
                     ADKFAAIQQLIKDTAIIKVKQYAPITENLRSDVLTDQKQLLEAGAFAFTNDGVGVQTA
                     GTMYLAMKEAAKNNVALVAHTEDDSLLFGGVMHKGTRSDELDLPGILSVTESTQIARD
                     VLLAEATGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVAPHHLILIDGSIPTDTAIY
                     KMNPPLRGAEDRAALIEGLLDGTIDCIATDHAPHG"
     gene            complement(1237..>1519)
                     /locus_tag="PLT73_09455"
     CDS             complement(1237..>1519)
                     /locus_tag="PLT73_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRM20635.1"
                     /translation="MMLLRVQTERHGGKAMRYTKAEYLDQFGLTVAREARMKETAIIM
                     HPAPVNRDVELADSLVECRRSRIVTQMKNGVFARMAILEAIVNGRTTNV"
BASE COUNT          308 a          386 c          393 g          422 t
ORIGIN      
        1 ggcgtatttg tcgctttcga aaggaagtct tcgctgcgga tttcgtattc gttggccaag
       61 tcgaagatgg cgatgtccgc ttgggaaccg atatccagtg ttcctgaccc cataccgaac
      121 aatgctgctg gcttgacggt catccaatca accagttgtt tcaaggtgaa gacgccgccc
      181 aagacgaagt tggaatacat taattggaaa gcagtctcga tgccgacgat gccgaatgga
      241 gcgccgacca taccgccttg ttttnnnnnn nnnngccgtg cggtgcatga tcggttgcga
      301 tgcagtcgat tgtgccgtcc aacaaacctt cgatcaaggc tgcgcggtct tcagcgccgc
      361 gcaatggcgg gttcattttg tagatggcag tatccgttgg gatggaacca tcgatcagaa
      421 tcaagtgatg tggtgcgact tcggcagtga catggatccc agcgcgcttg gcatcacgga
      481 tgacgcgtac gctctctttt gtggaaacgt ggcagacatg gtaatgacag cctgtcgctt
      541 ccgccaaaag cacgtcgcgg gcgatttggg tggattccgt aacgctcaaa atgcccggca
      601 gatccaattc gtcggaacgg gtgcctttgt gcatcacgcc tccgaacaac agcgaatcgt
      661 cttctgtatg cgcaacaaga gcgacgttat tctttgcggc ttccttcatt gccaaataca
      721 tcgttccagc tgtttggaca cctacaccat cgttagtgaa agcgaaagcg ccggcttcca
      781 ataattgctt ctgatcggtg aggacatcac tgcgcaagtt ctcggtgatg ggcgcgtact
      841 gtttgacttt gatgatggct gtgtctttga tcagttgttg gattgcagcg aatttatctg
      901 ctgtgtccgg aactggattc aggttcggca ttgcgcagac tgtcgtgaaa cctccgcgag
      961 cggctgcctg tgttcctgtt tcgattgtct ctttataggt gaatcctggt tcacgcaagt
     1021 gcacgtgcac gtcgatcaat ccaggagtta ccagaccgcc cttggcatcg atgactgtcg
     1081 catcctcagc cgttccttgc agatcctggc cgattgcggc aattttatcg tctttcacat
     1141 atatctctac aggaataagt tcctcttttt gacccaacat tttcgcattt tttatccaaa
     1201 ctgacatgtt atccctccac tggtgaattg ttattgttat acattcgttg ttcttccgtt
     1261 tacgatggct tccaagatgg ccattctggc aaagacgcca tttttcattt gcgtcacgat
     1321 ccgtgagcgg cggcattcca ctaggctgtc cgccaattcc acgtcgcggt tgacaggagc
     1381 gggatgcatg atgatggctg tttccttcat gcgtgcttcc cgagcgactg tcagtccgaa
     1441 ttggtctaga tactctgctt ttgtatagcg cattgctttt ccgccatggc gttccgtctg
     1501 gacgcggagc agcatcatg
//
LOCUS       DAOMUJ010000465         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22883_length_1518_cov_2.988380, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000465 DAOMUJ010000000
VERSION     DAOMUJ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22883_length_1518_cov_2.988380"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLT73_09460"
     CDS             <1..682
                     /locus_tag="PLT73_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20636.1"
                     /translation="DWRSIFWILNGMMLVSLTYFLKYLPKADHASTKLDLKNELQFFK
                     DGKTLLLLAYTLTMFMGYNAFYTYATPYLLLLFPSIEPLMSLILVGLGLASFTGNLIG
                     GHVSDAIGYAKSMMLGAVLQTAAMLLILVFQPSKWLSVLFIIGWLMSAWFTGLQLNTG
                     IAQVTENKSSFMLSINGSLIQLGGAFGASLAAVVINLSGIHSIVFVTLLTSLALILIQ
                     VVSMRKYP"
     gene            complement(775..1461)
                     /locus_tag="PLT73_09465"
     CDS             complement(775..1461)
                     /locus_tag="PLT73_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRM20637.1"
                     /translation="MKELYDGKTKTVLLDEANKEVYLLFKDSATGEDGVFDPGSNTVG
                     GSVAGKGKIGLTVSKYFFELMEKNGIPTHYLDADIEKGVMKVRQLTVPNLEFVLRYFT
                     AGSMCRRFTLPEGLPFDPPYLEVTLKDDEQGDPLITDRLCQMKGILKPGQYDEALDIL
                     TKVGAVLRDALAEMNLTLIDFKIEIGYDNDGKMYVVDEITPDIWRVKDEAGNIPNQID
                     CAQLILEKIQ"
BASE COUNT          355 a          373 c          339 g          451 t
ORIGIN      
        1 ggattggcgc agcatttttt ggatattgaa cggtatgatg ctggtttcgc ttacctattt
       61 cctgaagtat ttgccgaaag cggaccatgc ttccacaaaa ttggatctga aaaacgagct
      121 gcagttcttt aaggacggga aaacgttgct gctgcttgcc tatacgttga cgatgttcat
      181 ggggtacaac gccttctaca cctatgcgac gccgtatttg ctgttgcttt tcccttccat
      241 cgaaccgctg atgagcctga tcttggttgg tcttggcctt gccagtttca cgggcaatct
      301 gatcggcggc cacgtatcgg atgccatcgg atatgccaag tcgatgatgc tcggggcggt
      361 gctccaaaca gcagcgatgt tgctgatcct tgttttccaa ccgtcgaaat ggttaagcgt
      421 gctcttcatc atcggctggc tgatgagcgc ctggttcacc ggcctgcagc tcaacaccgg
      481 tatcgcccag gtgaccgaga acaaatccag tttcatgctc agcatcaatg gttccttgat
      541 ccaattgggc ggtgcgttcg gcgccagcct ggctgccgtc gtcatcaacc tcagcggcat
      601 tcatagcatc gtcttcgtca ccttgctgac cagtctagca ctcatcctga tccaagtggt
      661 ttcgatgaga aaatatcctt gagctcagat cgactatcac cactagaggg cgctttggaa
      721 tttttgcgga tatttaaaaa aggtgagatc ccgtattggg acctcacctt ttgcttattg
      781 aattttttct aaaatcaatt gcgcacagtc aatttggttg ggaatattgc ctgcttcatc
      841 tttgacgcgc caaatgtcgg gggtgatttc atccacgacg tacatttttc cgtcattgtc
      901 ataaccaatt tcaattttga aatcgattaa ggttaagttc atttcggcta aagcatctct
      961 aagaactgcg ccgactttcg ttaagatatc gagcgcttca tcgtattgcc caggctttaa
     1021 aatgcctttc atttggcaca agcggtcggt aatcaatgga tcgccttgct catcgtcttt
     1081 caaggtcact tccaaatatg gtgggtcaaa aggcaagcct tccggcaagg taaaacggcg
     1141 acacatgctg ccagccgtga aatagcgtaa aacaaattcc agattcggta ccgtcagttg
     1201 gcgaaccttc atgacacctt tttcgatatc cgcatccagg tagtgcgtag gaatgccatt
     1261 tttttccatc aattcaaaaa agtatttgga aacagtcaag ccaattttcc ctttgccggc
     1321 aacactgccg ccaactgtgt tggagcctgg atcgaagacg ccatcttcgc cggttgcgct
     1381 atctttaaac aataagtaga cttccttatt cgcttcatcc aataagactg tttttgtttt
     1441 gccgtcgtac aattctttca tgttttcccg ccttctctga aatatccagt gttttcaaaa
     1501 atgataccat tggatatt
//
LOCUS       DAOMUJ010000466         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22941_length_1516_cov_1.856947, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000466 DAOMUJ010000000
VERSION     DAOMUJ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22941_length_1516_cov_1.856947"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..820
                     /locus_tag="PLT73_09470"
     CDS             2..820
                     /locus_tag="PLT73_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRM20638.1"
                     /translation="MNEAKGLKKVLAVLGAFTLLSVSGCGQEQTAETDSSVQATTELA
                     SEAVVEETTEVAAEEHLDYGNQDEWTFESGEMQSPINIETGAAEAMVEDGSLTLDYAE
                     EIIDVVDNGHSIEMEDGGQATIAGRSFELTQFHLHSPSEHTLDGEYFPIELHFVHKAQ
                     DGRLAVIAVFFKEGTENAAFQSILDDVKANEESTVASGLSLNVAELLPANKSYYHYLG
                     SLTTPPLTENVEWYVMANPVEVSAEQIAAFNEYYQGNNREVQPLGERSVLKYEE"
     gene            1020..>1516
                     /locus_tag="PLT73_09475"
     CDS             1020..>1516
                     /locus_tag="PLT73_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20639.1"
                     /translation="MGEIAAKAASLILIIVIGYVIKRIGWVKAEDFPKFSKIVLRITL
                     PCALITSFNTFDITYNLLFLTAIGLIANLVLQITGYLVNQRKGGIAQAFGIINLGSYN
                     VGAFAMPYIAGLMGPQSIIFASLFDVGNSFGAAGIGYGWSRSVADEKQKTTLGGFLKL
                     MFLSPL"
BASE COUNT          413 a          356 c          403 g          344 t
ORIGIN      
        1 gatgaacgaa gcaaaaggat taaaaaaagt tttggcggtt ctgggggcat tcactttatt
       61 gagcgtgagc ggttgcggcc aagaacaaac ggcggaaacg gacagcagcg tgcaggcgac
      121 aacggaactg gcttccgaag cggtagttga agaaacaacc gaagtagcag ctgaagagca
      181 tttggattat gggaaccagg atgaatggac ttttgaatcc ggcgagatgc agtcgccgat
      241 caatatcgaa acgggtgcag ccgaagcgat ggtcgaagat ggctccctga ctttggacta
      301 tgcggaagaa atcatcgatg tggtcgacaa tgggcacagc atcgagatgg aggacggcgg
      361 gcaagcgacg attgccggac gcagttttga actgacgcaa ttccatttgc attctccgag
      421 cgagcatacc ctcgatggcg aatacttccc gatcgaactt cattttgtcc acaaggcaca
      481 ggacggacgc ttggctgtca tcgcggtttt ctttaaagaa ggaacggaaa atgctgcctt
      541 ccagtcaatt ttggatgatg tgaaggcgaa cgaagaatca accgttgcca gcggactcag
      601 cctgaacgtt gccgaactgc ttccagcgaa caaaagctac taccattacc taggatccct
      661 gacgacaccg ccgctgacag aaaatgtcga gtggtacgtg atggcgaacc ctgtggaagt
      721 ctccgcggaa cagattgcgg ccttcaacga atattatcaa ggcaacaaca gagaagtcca
      781 accattgggc gagcgttccg ttttgaaata cgaagaataa ccagtctttg cggcgatttg
      841 atcgtcttgg ctgaaaaaag ttccgttccc ccgatttgta ttggggctgg cggaactttt
      901 tttaattccc cgaccggagt caaagaaaat cgtaacgcga ttacaaaata cagaaagatc
      961 ggatgaaacg gcatattttt tgttataatt gaaggaaaaa cgcgagcagg gggaacgtaa
     1021 tgggggaaat agcagcaaaa gccgcatcat tgatattgat catcgtaatc ggctacgtca
     1081 tcaaacgaat cggatgggtc aaagcagaag atttcccgaa attttcgaaa atcgttttgc
     1141 ggatcacgtt gccctgcgca ctgatcacca gtttcaacac gttcgacatc acctacaatc
     1201 tgctgttttt gaccgcaatc ggcctcatcg ctaacttggt cctgcagatc accggctatc
     1261 tggtaaatca gcggaaaggc ggcatagcgc aggctttcgg aatcatcaac ctcggcagct
     1321 acaatgtcgg ggcctttgcg atgccatata tcgccggatt gatgggcccg cagtcgatca
     1381 ttttcgcatc ccttttcgat gtcggcaatt ccttcggcgc agctggaatc ggttatggct
     1441 ggtcccgttc ggtggcggac gagaaacaaa aaacgacctt aggcggcttc ctcaagctca
     1501 tgttcctttc accgct
//
LOCUS       DAOMUJ010000467         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_22992_length_1513_cov_1.997942, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000467 DAOMUJ010000000
VERSION     DAOMUJ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22992_length_1513_cov_1.997942"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..359
                     /locus_tag="PLT73_09480"
     CDS             156..359
                     /locus_tag="PLT73_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HRM20640.1"
                     /translation="MDGKLRKNIKIGALVDIVLKKDQRTGKLTRGHVKRLLTNSPNHP
                     HGIKVMLVEGDQVGRVQQIVEEA"
     misc_binding    503..732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            806..1456
                     /locus_tag="PLT73_09485"
     CDS             806..1456
                     /locus_tag="PLT73_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM20641.1"
                     /translation="MDLTFFETYLPIYFQGAGYTIGLSISAIVLGVILGTGLALMKMS
                     PNKFLSILANGYIQIVRGTPLLVQLFIIYYGLYVINIELPDFLSGVIAVSLNSAAYIA
                     EIIRSGIQAVDKGQMEAARSIGMSKSMAMQKIIYPQAIKNILPALGNEFVTLIKETSI
                     VSVLGLRDLMFAANTVRGATFLPFGPLVVAAVMYFIMTSIVSKLVDILEMRLKQSD"
BASE COUNT          460 a          316 c          360 g          377 t
ORIGIN      
        1 gctgggcgat aaattggaag ccgctgaagc tgcagcctta gcaggcgtgg acatcaaaac
       61 ggatcaggcg ctgcaggaca cgatccacta tttggacgaa gcggtcgatc acatcatcac
      121 actgagcaaa gagcttgctt aagaaagagg aaaatatgga cggaaaactc agaaaaaaca
      181 tcaaaatcgg cgctttggtc gatatcgtct tgaagaaaga ccaacgcacc gggaaattga
      241 caagagggca tgtgaaacgg ctgttgacga acagccccaa tcatccgcac ggcatcaaag
      301 ttatgctggt tgaaggcgat caggtcggac gcgtgcagca aatcgtagaa gaggcataaa
      361 ttgtagctaa aaaatgagaa aagaatcgag ttgtcgcgtc ttttcacaga aaaaaagggt
      421 cagatgaaaa tccaatgata gtttttttgt tgacagttct tttcaaatct tatattatga
      481 tttcatacca caactgaata gaacaaagtc agtgatgaag agagtagatt tgaatatttt
      541 ctccagcgag ccgggacaga gtggaagccc agcagtaaaa tatcagattg aagcgcactt
      601 cggatgacga aaaggtgaac gttgagtagc cttttacgca ctttatgcgt tattttaatc
      661 gaggtggatt tgttgtttac aacagatcaa ctagagtggt accgcggaat caaacattga
      721 cccgtctctt gtagcaatac aaggggcggt tttttagtac ccaaagcacc accagcaaaa
      781 acataaaaaa ggggattata tacaaatgga tttaactttc tttgagacct atctgccgat
      841 ctatttccaa ggcgcgggtt acacgattgg cctttcgatc agtgcgattg tactgggcgt
      901 catcttgggg acgggattgg cattgatgaa aatgtcgccc aacaagttcc tcagcatatt
      961 agcgaatggg tacatccaaa ttgtgcgggg cacaccactt ttggtgcaat tattcatcat
     1021 ttattacggc ctgtacgtca tcaatatcga attgcctgac ttcttgtccg gtgtcatcgc
     1081 ggtatcattg aactcggcag cctacatcgc tgaaatcatc cgttccggaa tccaagcagt
     1141 cgataaaggc cagatggaag ccgcccgttc gatcggaatg agcaaatcaa tggcgatgca
     1201 aaaaatcatt tatccacaag ccatcaagaa tatccttcct gctttgggta acgaattcgt
     1261 gacgctgatt aaagaaacat ccatcgtttc tgtgttgggc ttacgcgact tgatgttcgc
     1321 cgccaatacg gtccgcggtg cgacattctt gccattcggc ccgctagtgg tcgctgccgt
     1381 gatgtacttc atcatgacat caatcgtctc taaattagtg gacattctgg aaatgaggtt
     1441 gaaacaaagt gattaaaaca gaaaaactta cgaaatcttt cggggacaaa caagtcctga
     1501 aaggcataga tga
//
LOCUS       DAOMUJ010000468         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23002_length_1512_cov_2.300618, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000468 DAOMUJ010000000
VERSION     DAOMUJ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23002_length_1512_cov_2.300618"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..447
                     /gene="msrB"
                     /locus_tag="PLT73_09490"
     CDS             22..447
                     /gene="msrB"
                     /locus_tag="PLT73_09490"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003627616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRM20642.1"
                     /translation="MDNKEKLLKRLTPLQYEVTQNEATERPFSGEYDDFYEKGIYVDV
                     VSGEPLFSSADKYDAGCGWPSFAKPISTLKELEDRKLMRKRTEVRSSQADSHLGHVFE
                     DGPAELGGLRYCINSAAMRFVPYDQLDAEGYSEYKQLFE"
     gene            614..1225
                     /gene="ruvA"
                     /locus_tag="PLT73_09495"
     CDS             614..1225
                     /gene="ruvA"
                     /locus_tag="PLT73_09495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRM20643.1"
                     /translation="MYEYIKGKLVFVGPLYVVLENGGIGYQLQVANPFRFSGSLNQDV
                     TFYIYQAVREDAITLFGFKDYTEKQLYLKLISVSGIGPKSGLSILASDDHLGLIQAIE
                     TDNVGYLVKFPGVGKKTAAQIILDLKGKLGELLPDTYWLEEHPVHETTRGQSAVLTEA
                     LEALTGLGYSDREVKKILPLLEKENYSNTEEVLRAAFKLLLKG"
     gene            1231..>1512
                     /locus_tag="PLT73_09500"
     CDS             1231..>1512
                     /locus_tag="PLT73_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015473544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20644.1"
                     /translation="MTAENRIVSGNTEDLTEDLIEKTLRPQMMAHYIGQDKVKNELKV
                     YIQAAKARSEALDHVLLYGPPGLGKTTLAMVIANELDVRIHSTSGPAIER"
BASE COUNT          433 a          309 c          402 g          368 t
ORIGIN      
        1 agaaaggggt ctgatactaa gatggacaac aaagagaaac tattgaagcg cttgacgcct
       61 ttacaatatg aagtgacaca gaacgaagct acggaaagac ctttttccgg cgagtacgat
      121 gatttttatg aaaagggcat ctatgtggat gtcgtgagcg gagaaccatt gttcagttct
      181 gcagacaaat atgatgcagg ctgcggctgg ccttcttttg caaaaccgat ttccactctg
      241 aaggagctgg aagaccgtaa gttgatgcgc aaacgcacgg aagtccgcag ttctcaagcc
      301 gactcacatc tggggcatgt ttttgaggac ggtcctgcgg agttgggcgg cttgcgctac
      361 tgcatcaatt ctgcagccat gcgcttcgtc ccttacgatc aattggatgc ggaaggctac
      421 tctgaataca aacaattatt cgaatagtca gcaaagataa gtcccatggc taccggtcct
      481 gggacttttt gttgcttcat gcagaccaaa cgattgttcc cacataaatg gctgatcggc
      541 tgtcatttcc gttcggtttt atgttatgat aggaccgaat gaaaatcagg aaatgaagag
      601 taggagtcag atgatgtacg aatacataaa aggtaaatta gtctttgtgg gaccgctcta
      661 tgtggttctg gagaatggtg gcataggcta ccaattgcag gttgcgaatc ctttccggtt
      721 ctccggaagt ttgaaccagg atgtgacatt ttacatctat caggcagtcc gcgaggatgc
      781 aatcaccctg ttcggtttca aggactacac ggaaaaacag ttgtacttga aattgatcag
      841 cgtctctggg atcggaccga agagtggctt atcgatattg gcgagcgatg atcacttagg
      901 cctgatccaa gcgattgaaa cggataatgt ggggtatttg gtgaaatttc ctggtgtagg
      961 caaaaaaacg gctgcgcaga tcatcttgga cttgaaaggc aaactcgggg aattgctgcc
     1021 ggatacctat tggctggaag agcatcccgt ccatgaaacc actcgcggac agtctgctgt
     1081 cttgacggaa gccttggaag cattgaccgg tttgggctat tcggatcgag aagtgaagaa
     1141 aatactgccg ctattggaaa aggaaaacta ttccaacaca gaagaagtgc ttcgtgcggc
     1201 tttcaagcta cttttaaaag ggtgatatag atgacagctg aaaacagaat cgtttccgga
     1261 aatacggaag atctaacaga agatttgatt gaaaaaacat tacggccgca aatgatggcg
     1321 cactatatcg gccaagacaa agtgaagaat gaactgaaag tttatattca agccgcaaaa
     1381 gcgcgcagtg aagccttgga tcacgtgcta ttgtacgggc cgcccggatt gggaaaaacg
     1441 acattggcga tggtcattgc caatgaattg gatgtccgga tccacagcac gagcggaccc
     1501 gcaatcgagc gg
//
LOCUS       DAOMUJ010000469         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23024_length_1511_cov_2.297390, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000469 DAOMUJ010000000
VERSION     DAOMUJ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23024_length_1511_cov_2.297390"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..835
                     /locus_tag="PLT73_09505"
     CDS             95..835
                     /locus_tag="PLT73_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRM20645.1"
                     /translation="MAYTKIIAHRGSRGNRPENTLEAFQEALKVSSDGIELDVHLSKD
                     GQVVVIHDETVDRTTSGKGYVKDMTLKQLKELDAGSWFDPKYSNARIPTLQEVLDLLA
                     ENHFSGVLNVELKTDVFQYSAIEEKVLALAEPYLAQFSVIYSSFHYETLKKIKELRQD
                     SKIALLFSKKGKQRLDLGEGIAVEGWHPKFSILRSLPPFEQSKIPLRVWTVNRKVEMQ
                     VCLRKNIDSMITDYPERALQMRKVIQGV"
     gene            828..>1511
                     /gene="miaA"
                     /locus_tag="PLT73_09510"
     CDS             828..>1511
                     /gene="miaA"
                     /locus_tag="PLT73_09510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRM20646.1"
                     /translation="MSETKNNLEKIMVIVGPTAVGKTALSIRLAKKYNGEIINGDSMQ
                     VYAGLDIGTAKVTEEEAEGIPHHLLDIIPVEQDYTASDFKRDARQAIESILARGKLPI
                     VVGGSGLYIEGLLFDMQFGGVAAENPDYRKQLEAELERTDAMCIWKQLQAKDPKAAEQ
                     IHPNNSRRVIRALEVIHFSGKRFSDQTDRETAPHYDALLIGLDTDRGILYDRINRRVD
                     LMTDKGLVEE"
BASE COUNT          443 a          328 c          411 g          329 t
ORIGIN      
        1 catgcgaaag aattgcttga gttggccgat accgaaaaaa acaaagcgta acagaagacc
       61 tgcgcagtag aacagactgg aaggattgag gagattggca tacactaaaa tcatcgcgca
      121 tagaggcagt cgcggaaacc ggccagaaaa tacgctggag gcttttcagg aagctctgaa
      181 agtttccagc gacggtatcg aactggacgt ccatttaagc aaggatggtc aggtagtggt
      241 catccatgac gagaccgtgg accgcacgac atccggaaaa ggctatgtaa aagacatgac
      301 gctgaagcaa ttgaaggaac tggatgccgg cagctggttc gatccgaaat acagcaacgc
      361 ccgtatcccg acgcttcagg aggtattgga cctattggcc gagaatcatt tttcaggcgt
      421 ccttaatgtt gaattgaaga cggatgtgtt tcaatattcc gcaatcgaag aaaaggtgct
      481 tgctttggca gagccttatc ttgcccaatt ttcggtcatc tactccagtt tccattacga
      541 aaccttgaaa aaaatcaagg agttgcgcca agactcgaaa attgccctgc ttttcagcaa
      601 gaaaggcaag caacgactgg atctaggtga aggaattgct gtggaaggtt ggcatccgaa
      661 attctcaatt ctgcgcagtc tgcccccttt cgaacaatca aagattccgc ttcgtgtttg
      721 gacggtgaac cgaaaggtcg aaatgcaggt ttgtctgcgc aaaaacatcg attcgatgat
      781 tacggattat ccggaacgcg cactgcagat gagaaaggtg attcaaggtg tctgaaacaa
      841 agaacaattt agagaaaatc atggtcattg tcggaccgac agcagtgggg aaaaccgcac
      901 tgagcatccg actggcaaaa aaatacaacg gtgaaatcat caatggcgat tccatgcaag
      961 tgtacgcagg tttggacatc ggtacggcta aagtgaccga ggaagaggct gaaggcatcc
     1021 cgcaccatct tttggatatc attccagtgg agcaggatta cacggcgtcc gatttcaaaa
     1081 gggacgccag acaagcaatc gagtcgatcc tggcgagagg caagctaccg atcgttgtcg
     1141 gaggttcggg actttatata gaaggactat tgtttgatat gcagtttggc ggggtggctg
     1201 ccgaaaatcc tgactaccgg aaacaactgg aggctgagtt ggagcggact gatgccatgt
     1261 gcatatggaa gcaattgcag gcgaaggatc ccaaagcagc tgaacagatc catccgaaca
     1321 acagcagaag ggtgatccgc gctttggaag tgatccactt cagcggaaag cgtttctcgg
     1381 atcagacaga tcgggagaca gctccacatt atgacgcctt gctgattggg cttgacaccg
     1441 atcgcggtat cctttacgat cgcatcaaca gacgggtgga tctgatgact gataagggtc
     1501 tcgttgaaga a
//
LOCUS       DAOMUJ010000470         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23030_length_1511_cov_1.947115, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000470 DAOMUJ010000000
VERSION     DAOMUJ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23030_length_1511_cov_1.947115"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLT73_09515"
     CDS             complement(<1..576)
                     /locus_tag="PLT73_09515"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HRM20647.1"
                     /translation="MKKEDFINKVRGGIIVSCQAQPGEPLYTEEGGIMPLMALAAEQA
                     GAVGIRAESVRDIQQIKEKVDLPIIGIIKRDYPPQAPFITATMKEIDELVETGVEVIA
                     LDCTLRERYDGKTINAFIKEMKEKYPDQLFMADIATLEEGINAFNEGIDFIGTTLSGY
                     TAESTQQDGPDITLMKKLVEAGVDVIAEGKIH"
     gene            783..1286
                     /locus_tag="PLT73_09520"
     CDS             783..1286
                     /locus_tag="PLT73_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM20648.1"
                     /translation="MANYLLKNQEQAVYQTMKDVTEAVKVGDATLIRFSQKIGFNGFS
                     DLKIAIAKEEFASQYEGDNDHQFYDRIMENQIKVLESTRLLLDPEVVLHAIEALTKAK
                     RIYIIGVGSSGLSAASLEKMLLRVGVHANHIVDPHFQAHALALLGPEDLVICFSLSGR
                     TIDKTLN"
BASE COUNT          401 a          309 c          345 g          456 t
ORIGIN      
        1 atggatttta ccttcggcga tgacatccac accggcttcc actagttttt tcatcaaggt
       61 gatatccggc ccatcctgtt gggtgctttc cgctgtgtaa ccgcttagcg ttgtgccgat
      121 aaaatcgatt ccttcattga aagcgttgat tccttcctcc aaggtggcga tatccgccat
      181 gaacaattga tcgggatatt tttctttcat ttctttgatg aaggcattga ttgttttgcc
      241 gtcatagcgt tcccgcaaag tgcaatccaa ggcaatgact tctacacccg tttcaaccaa
      301 ttcatcgatt tccttcatgg tagccgtaat aaatggtgct tgaggggggt aatcacgttt
      361 gatgatcccg atgatcggta aatcaacttt ttccttaatt tgttgaatat cccttaccga
      421 ttcggcacga attccgactg caccagcttg ttcagctgca agagccatca aaggcatgat
      481 gccgccttct tctgtgtata aaggttctcc cggttgcgct tgacaagaga caataatgcc
      541 gccacggact ttgttgatga aatcttcctt cttcatggat atttcctccg ttatttattt
      601 tattccggag ttaatctccg taaatgaaat attccacacg ctttcatgtt tgtaaacagg
      661 taatttgcat ttttaaaagt tcgtgtataa tgagagtcat aaacaggaaa aggaatgact
      721 ttatgggact agctcaactg attcaagaaa aatatcacgc gttgacgaaa tcctagaaac
      781 ggattgcgaa ttacttattg aaaaatcaag aacaggctgt gtatcagaca atgaaggatg
      841 tgaccgaggc agtaaaagta ggcgatgcca cactgatccg cttttctcag aagatcggtt
      901 tcaatggctt ttctgatttg aaaattgcta tcgccaagga ggaattcgct agccagtatg
      961 aaggggataa cgatcaccaa ttttacgatc ggattatgga gaaccagatc aaggttttgg
     1021 aatcgacccg actgttactc gatccggaag tggtcctgca tgcgatcgaa gccctcacga
     1081 aagcaaaacg gatctacatc atcggtgttg gatcgagtgg tttgtcggcg gcttcgttgg
     1141 aaaagatgtt gctccgggta ggtgtgcatg cgaaccatat cgttgatccg cattttcaag
     1201 cccatgcctt agcattgctg ggaccagaag atctcgtgat ctgtttctct ttgagcggcc
     1261 gtaccattga taagacactg aattaatttt gcagcggcat tatccgctct ttcctgcagc
     1321 atgcagtgga gacgtgtggc catagtgtaa ttatattatg gaagtgtgca tgatttatgt
     1381 atgaatgaaa gggaataggc tgtttatgcc agtttattga acataaattc ctgagcggaa
     1441 tagttgcgca aaaatatgct atactgagag aaagagagtt ggatagttaa gcgatttttc
     1501 ggtatcctga t
//
LOCUS       DAOMUJ010000471         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23046_length_1510_cov_2.149141, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000471 DAOMUJ010000000
VERSION     DAOMUJ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23046_length_1510_cov_2.149141"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLT73_09525"
     CDS             <1..483
                     /locus_tag="PLT73_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM20649.1"
                     /translation="TQIGFLEGKELTNDKEMLVRDLRSELFLAELRKRGWYRDKYHRS
                     GSFSTSSGYEMMNQLLNDNGKQLPTFLFAENDAIAIGALRALQEADIAVPDQISIVGF
                     NDSKVAKYVYPPLSSIRVDTREMGRTGVSLLLDRIAYPRKISKKISLSTEWIKRASTK
                     "
     gene            complement(590..823)
                     /locus_tag="PLT73_09530"
     CDS             complement(590..823)
                     /locus_tag="PLT73_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRM20650.1"
                     /translation="MDSGMGDKLKGMKDKVVGKIKEEYGDLVDDPEKELEGKLQQARG
                     EAIQKKGEIKEDLHDKADDLADKANDKIDEWKK"
     gene            999..>1510
                     /gene="tsaB"
                     /locus_tag="PLT73_09535"
     CDS             999..>1510
                     /gene="tsaB"
                     /locus_tag="PLT73_09535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM20651.1"
                     /translation="MKILAIESSNQTMSAAVCENGRLLAEVTTNGNLQHSTQLMPAVD
                     HVMKLAGWKPADLECIAVAKGPGSYTGVRIGATIAKTLAWTLAIPLVPISSLKVIAGN
                     CEGAAHKLVPMIDARRGNCYTAVYQFEDNLLVERIADTHIASEEWFQRLLAEEGTYLF
                     VGEDISKYRER"
BASE COUNT          386 a          350 c          350 g          424 t
ORIGIN      
        1 acccaaatcg gttttttgga gggcaaggag ctgacgaacg acaaagaaat gctggtccgt
       61 gatctgcggt ctgaactctt ccttgctgaa ctgcgcaaac ggggctggta ccgggataag
      121 tatcatcgta gcggaagctt cagtacttct tcgggatacg agatgatgaa tcagctgctt
      181 aacgacaatg ggaaacaact tccgactttc ttgtttgccg aaaatgatgc gattgctata
      241 ggagccttac gtgcgcttca agaagctgat attgcggtgc cggatcaaat ttcgatagtc
      301 ggattcaacg acagcaaggt tgccaaatac gtttacccac cgttgtcgag catccgagtc
      361 gatacgcgtg agatgggcag gacaggtgta tccttattgc ttgaccgcat cgcctatccg
      421 cgcaaaatat ccaaaaaaat cagcttatcc accgaatgga ttaagcgtgc atcgacaaag
      481 tgattccacc aatcaatggt gaattacttt gaaaaaagaa taagccgccc caacaaaccg
      541 atttaggttg ttgggacggc ttataatctt attctttcaa aaatgagtct tattttttcc
      601 actcatcgat tttatcattt gctttatctg ctaaatcatc ggctttgtca tggagatctt
      661 ctttgatttc accttttttt tgtattgctt cgccgcgtgc ttgttggagt tttccttcga
      721 gttctttttc tgggtcgtcc actaggtcgc cgtattcctc tttgattttg ccgactactt
      781 tgtccttcat gccttttaat ttatcgccca tacctgaatc catgatgttt cctccttttg
      841 atttcacact gattggcttc gcttactata acctatttta aagcatttat tatcagaatc
      901 aagcaatagc ccttgagacc aactgggcat ccagcgtcct tcaggaagta tttagaagga
      961 tgcatacttt ttatcgcctt gattaaatgc tatactagat gaaaatatta gccatcgaat
     1021 cctcaaacca gaccatgagt gcggctgtat gcgaaaatgg ccgcttgctc gcagaagtca
     1081 ccaccaacgg aaatcttcag cacagtaccc aattgatgcc agcagtggat catgtcatga
     1141 aacttgccgg atggaaacct gccgatttgg aatgcatcgc agtcgctaag gggccgggct
     1201 cctacacagg agtcaggatc ggagcgacca tcgcgaaaac tttggcatgg acgttagcga
     1261 ttcctttggt gccgatctcc agcctgaagg ttatcgccgg caattgtgaa ggggctgcgc
     1321 acaagcttgt cccaatgatt gacgcacgcc ggggcaactg ttacacagcg gtttatcaat
     1381 tcgaggacaa cttgttggtg gaacggattg ccgacacaca tatagccagt gaagagtggt
     1441 tccaacgctt gttggcggaa gaaggcacct atctgtttgt gggagaagac atcagcaaat
     1501 accgcgagcg
//
LOCUS       DAOMUJ010000472         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23049_length_1510_cov_2.033677, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000472 DAOMUJ010000000
VERSION     DAOMUJ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23049_length_1510_cov_2.033677"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLT73_09540"
     CDS             <1..448
                     /locus_tag="PLT73_09540"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRM20652.1"
                     /translation="EVAQPGDIIVIDAKGSCNNGVWGDNRSRVAKAIGLAGVVIDGAF
                     RDIEENEALGFPIYARANTCGRSTRGAKGEVNVPISCGGVSVRPGDIIVGDRNGVVVI
                     PLEHAEEIIQAAQEQVDKMEAQIAKFEATGQVVPDSLEKELKRLGY"
     gene            complement(513..1508)
                     /locus_tag="PLT73_09545"
     CDS             complement(513..1508)
                     /locus_tag="PLT73_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM20653.1"
                     /translation="MHEDRIGDAARIGGRKPIMLTFNGKAALTISVDIAPNYVEGLIA
                     YVDGKVLYEAETKGIKVSAENALSHITELVEKLMQHAEETPHGIIGLCVAIHGLVDQE
                     EIVFTPNYTLQSDLKVQLEDVYPFYVHLENEANLAALGEYTFGSSSDSVVSLSIHTGI
                     GAGIVENGKLRTGKTGKAGEIGHSILYPDGRKCPCGNEGCLEQYASHQAIYSELEQAL
                     GLPDVNSSLVIERYKKGDPTVQKVLRTNAHYLSIAVNNLAVIYEPDLVVINSSIYWQI
                     PELITILKGDLKGKFSCDTRVISSSLKRKATLFGGVAKSTQNFLNIQNLKMNTEN"
BASE COUNT          349 a          378 c          421 g          362 t
ORIGIN      
        1 ggaggtagcg caaccgggtg acatcatcgt catcgatgcg aaaggtagct gcaataatgg
       61 cgtttggggc gacaaccgca gccgggtggc caaggcgatt ggtttagccg gtgtcgtaat
      121 cgacggtgcc ttccgcgata tcgaagaaaa tgaagcactc ggcttcccga tttatgcccg
      181 ggcgaacacc tgcggcagaa gcactagggg cgcgaagggg gaagtcaatg taccgatttc
      241 ctgtggcggc gtgtcggtgc gccccgggga catcatcgtc ggggaccgta atggggtcgt
      301 cgtgattccg ttggagcatg ccgaggaaat catccaagca gcccaagagc aggtggataa
      361 gatggaggcg cagatcgcca aattcgaagc gaccggccag gtcgtcccgg acagcctgga
      421 aaaggaattg aaaagattag gctattaaaa gcggaaaaaa ttcaatcaaa gaagacaaaa
      481 ccacctgtgc agggagcgca ggtggttttg ttttagtttt cagtattcat tttcaggttt
      541 tggatgttca ggaagttctg tgtggacttg gccacaccgc cgaacaaggt cgctttccgc
      601 ttgagcgagc tgctgatgac acgcgtatcg cacgaaaatt tacctttgag atccccttta
      661 aggatagtga tcaattcagg aatctgccaa taaatggaac tgttgatcac aaccaagtcg
      721 ggttcgtaga ttaccgccag attattgacg gcaatactca aataatgcgc attggtccgc
      781 agaacttttt ggacagtcgg atcgcctttt ttgtagcgct cgatcaccaa ggaagaattg
      841 acgtccggca acccgagcgc ctgctccagt tcgctgtaga tagcctgatg ggatgcgtac
      901 tgttccaagc agccttcgtt gccgcaaggg cacttccggc catccgggta gaggatgctg
      961 tggccgattt cacccgcttt tccggttttg ccggtccgca gtttcccatt ttcgacgatg
     1021 cctgcgccaa tgcccgtatg gatgctcaga ctgacgacac tatccgaact ggatccgaaa
     1081 gtatactcac cgagcgccgc cagattcgct tcattttcca aatgcacgta gaagggatac
     1141 acatcctcca gctggacttt cagatcgctc tgcaacgtat agttgggcgt gaaaacaatc
     1201 tcttcctgat cgacgaggcc gtggatcgca acgcagagtc cgatgatgcc gtgcggggtt
     1261 tcctctgcat gctgcatcag cttctcgacc agttccgtga tatgggagag ggcgttttct
     1321 gcagaaactt tgatgccttt ggtttcggct tcatacagga cttttccgtc gacataagcg
     1381 atgagtccct ctacataatt gggtgcgatg tccaccgaaa ttgtgagcgc ggctttcccg
     1441 ttgaaagtga gcatgattgg cttcctgccg ccgattctgg ctgcatcacc gatccggtct
     1501 tcgtgaatca
//
LOCUS       DAOMUJ010000473         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23079_length_1509_cov_1.305365, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000473 DAOMUJ010000000
VERSION     DAOMUJ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23079_length_1509_cov_1.305365"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1509)
                     /locus_tag="PLT73_09550"
                     /pseudo
     CDS             complement(1..1509)
                     /locus_tag="PLT73_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754685.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          279 a          431 c          403 g          396 t
ORIGIN      
        1 gcccgttttg cctttcgcca cgtgggcacg gacgattttg ccgtcgattt ctttcgaaat
       61 cagcgtgttg ccggtgttct tcgtccaggc agcgatgtcc gccaggaagc cgaagtcgga
      121 tgcttcgatg atcagttcct gaccgtcgac cattttgtcc atgttctctt tgactttcag
      181 tatcggaccc gggcactgca gtccgcaggc atcgacttgg atcgtgttgc cgaccggttg
      241 ggctggcttc gcgtgtgtcg gttccacatg cacggcctct tccggcgctt cctctttata
      301 attcaaagtc gtattcgctt gtttgtaagt cttgtaaccg ccgtccaggt tcttgacggt
      361 gaagccgttc tgcatcagga tgcgggccgc gttgtagccg cggtggccga cttggcagaa
      421 cacatggatc gtcttgtctt tcggcaattc gctcatgcgc gcgcggatat cgcccaacgg
      481 aatcgtgtag gcattgccca acacgccggt cgccaactcg aagtcttcgc ggacatcgag
      541 gaagaatgcg cccgcttcga tcaggccgtc gacttcatgc cattggaacg tgtcgatttt
      601 gccttcgagg atgttggaag ccacatagcc ggcgatgttg gccggatctt ttgcggatga
      661 atacggcggt gcgtatgcca attccaaatc cggcaaatcg tagaccgtcg cgcccaattt
      721 catggctgtc gcgatcacgt cgacgcgttt ttccacgcct tccatcccga ctgcctgcac
      781 acccaaaatt ttgccgtcca agccgaacaa caatttgatg tccaacggcg aagcccctgg
      841 gtaataacca gcatgggaat tcggatgaat gtgggccact ttatagtcgg tgccgcaacg
      901 tttcaaggtt ttctcgttgt ttccggctgc cgccaccgtc aattcgaata ctttcgcgac
      961 ggaagttccc atcgtgcctt tgtagcggac atccatgccg ttcaggtggt cagccaccaa
     1021 gcggccttgg cggttcgctg gccaagccaa cggaacggca gtcttgctgc cgttgacgaa
     1081 gtcggtaact tcgatgacgt cccctaccgc gaagatatcc ggatcggatg tctgtagttg
     1141 gtcgttcacg acaattgccc ctctgaagcc tgtttccagc tcggcatcgg tcgccaattc
     1201 gttctcaggt gtcacaccga tcgaaaggat cgtcatgtcc gtatccaaca ctttgccgct
     1261 gttcaagcgg acttttgagc cgtttgacgc cgtcgctcag gatcaggttg acgttgtgca
     1321 tatcgatatg cgcgtgcacg gattgcgcca tctcgaagtc aatcggtgcc atgacttggt
     1381 cgctcatttc caccaaggat acttggatgc ccaattccca taggttttcc atcatttcca
     1441 agccgatgaa gccgccaccg atgaccgtcg cgcgtttgac ttggtgctcg ttgatgtagg
     1501 ctttgatct
//
LOCUS       DAOMUJ010000474         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23092_length_1508_cov_2.039917, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000474 DAOMUJ010000000
VERSION     DAOMUJ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23092_length_1508_cov_2.039917"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLT73_09555"
     CDS             complement(<1..589)
                     /locus_tag="PLT73_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HRM20654.1"
                     /translation="MQEVYQHFRKEEQPFIDSAQSWITQAEETYTPYLTDFLDPRQQY
                     ILEMLVGKKGEVHLQFYGGYEAAERKRAIICPEYFSPNQSDFEIELTEVVYPSKFASL
                     THGKILGTLIGTGMKRELFGDILSDGTRWQFLLASNIASYVHSQITKIGKVSVRLESR
                     TYTDLIMPIDDWTIVHDTVSSLRLDTVIAAIYNISR"
     gene            complement(629..916)
                     /locus_tag="PLT73_09560"
     CDS             complement(629..916)
                     /locus_tag="PLT73_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRM20655.1"
                     /translation="MTQILIWLLQIIRWGISAYSTVLLVYALMSWLPGARSSKFGYFI
                     SSIVEPYLDIFRRLIPSVGMVSFSVVAGILFLNLVEYGAQVVILFLIRLLN"
     gene            complement(929..1417)
                     /locus_tag="PLT73_09565"
     CDS             complement(929..1417)
                     /locus_tag="PLT73_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRM20656.1"
                     /translation="MGLKDLFRKYFVIEDEDDGYEDDGYEEVDEPSTVVPVRMSERNE
                     EGAPSQRSTQKRSTKAKVIPMNQQTMTEKASIHVVEPRVYSEVEKIADNLLKNQAVLL
                     NFKRIDAEQAKRIVDFLTGTVYAIGGEIQRVGDEIFLCTPASVGVEGALAETLEEERS
                     FY"
BASE COUNT          390 a          392 c          290 g          436 t
ORIGIN      
        1 gcctggatat gttgtagatg gcagcaatca cagtatccaa gcgcagagaa ctgacagtat
       61 catgcacaat cgtccaatca tcgataggca tgatcagatc agtatacgtc cgactctcta
      121 ggcggacact gaccttgcct atcttggtga tttgcgaatg cacgtagctt gctatattcg
      181 acgccaataa aaactgccag cgcgtcccgt cagacaggat atccccgaaa agttcccttt
      241 tcatgccagt cccgatcagc gtccccagta ttttcccatg ggtaagactg gcaaattttg
      301 aaggatacac aacctcagta agttcgattt caaagtcgct ttgattcgga ctgaaatact
      361 cggggcaaat gattgcccgt ttacgttcgg ctgcttcata gcctccataa aattggagat
      421 ggacttcacc tttttttcca accagcattt caagaatata ctgctgtctt ggatccaaga
      481 aatccgtcaa atacggtgta taggtttcct ctgcttgcgt aatccaagat tgggcactgt
      541 ctataaatgg ctgctcttcc ttgcggaaat gttgataaac ttcttgcata cgaatccctc
      601 actttttcgt ttgatgaaaa aatgtccgtc aattcagcaa tctgatcagg aacagaatca
      661 cgacttgtgc tccatactcc accaaattca ggaataggat ccccgccact acactgaagc
      721 tcaccatacc cacagatggg atcaatcttc tgaatatatc caggtaaggc tccacaatac
      781 tggagataaa gtatccaaat ttggaacttc tggctcccgg taaccaactc atcaatgcat
      841 ataccagcaa taccgttgaa tacgcggata tgccccaacg aataatctgt aaaagccaaa
      901 tcaatatttg tgtcattttg cttcctttct agtaaaacga acgctcttct tcaagtgttt
      961 cggccagtgc gccttcgact ccgactgaag caggggtgca taaaaagatt tcatcaccaa
     1021 cgcgttggat ttctcccccg atcgcatata ccgttccggt gagaaagtcc acgatccgct
     1081 ttgcttgttc cgcatctatc cttttgaaat taagcagtac tgcttgattc ttcaacagat
     1141 tatcggcaat cttttccact tcggaataga ctctcggctc cacaacatgg atacttgcct
     1201 tttcggtcat tgtttgttga ttcataggaa ttacctttgc tttcgtcgat ctcttttggg
     1261 tgctcctttg agatggggcc ccttcttcat tgcgctcgga catacggact ggaacgacag
     1321 tcgatggttc atctacttcc tcgtatccgt catcctcgta tccgtcatcc tcgtcttcaa
     1381 ttacaaagta ctttcggaat aaatctttca gacccataag acccctcact tcttcttcgt
     1441 ctatgcttct gtttcagcaa agaatgccga tccgacacgt acgaaggtcg caccttcggc
     1501 aatcgcta
//
LOCUS       DAOMUJ010000475         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23110_length_1507_cov_2.240358, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000475 DAOMUJ010000000
VERSION     DAOMUJ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23110_length_1507_cov_2.240358"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLT73_09570"
     CDS             <1..474
                     /locus_tag="PLT73_09570"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRM20657.1"
                     /translation="GSAGGLNTALHVGDVVVADKVAYHDVDATAFGYSVGQVPQMPHY
                     YTADKKVVEKITRAAEKVGLHAVQGTIVSSDSFIASESGTNRIKEHFPDAYATEMEGA
                     SIAQACYVLGTPFAIIRAISDGASDGAALSFDEFIVEAGKKSAEMVIELLKNNGA"
     gene            635..>1507
                     /locus_tag="PLT73_09575"
     CDS             635..>1507
                     /locus_tag="PLT73_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRM20658.1"
                     /translation="MRQELVIGQGEKPAIIHLDKLNRHGLIAGATGTGKTVTLKVIAE
                     KLSKAGIPVFLADVKGDLASIAEPGELSEKILERAAKLQPEDFEPRAYPVELLDIFGA
                     NGVPVRTTISDMGPILLSRLLGLNETQEGILNIAFKVSDEKQLLLIDIKDLRAMLNFV
                     GDNATELRREYGNISKQSIGAILRGLLVIEEQGGDLFFGEPMFDIQDLLKTDAAGRGY
                     VNILMANRLFMSPKLYSTFLLWFLSELYEQLPEVGDLEKPKIVFFFDEAHVLFQDVPD
                     VLEEKIELIVRLIRS"
BASE COUNT          396 a          321 c          413 g          377 t
ORIGIN      
        1 ggttccgccg gtgggttgaa cacagctttg catgtaggcg atgtcgtcgt agcggataag
       61 gttgcttatc atgatgtgga cgctaccgcc ttcggttatt cggttggaca agtaccacag
      121 atgccgcatt attatacggc cgataaaaaa gtcgttgaaa agattacgcg ggcagctgaa
      181 aaagtcggct tgcacgctgt ccaaggaacg attgtctcaa gtgattcctt catcgcgtct
      241 gaaagcggca caaaccgcat caaggaacat ttccctgatg cctatgcgac cgaaatggag
      301 ggcgcatcga tagcacaggc ttgctacgtt ctggggaccc ccttcgccat cattcgtgcc
      361 atttcggacg gcgcaagcga tggtgcagcc ctgagtttcg acgaattcat cgttgaagcc
      421 ggaaagaaat cagcggaaat ggtcatcgaa ttactgaaaa ataacggagc atagtccgaa
      481 attgaaccgc tcatccgata atggctgagt ggtttttgct ttctgcagaa ttttggagcg
      541 ctgttacttc agaaaaggaa gggcttactg ttgtaagggt ttttcaaaat actgtacaat
      601 aaggatgaaa tgtcaggcta aggagggatt ttggatgcgc caagaactgg ttatcggaca
      661 aggggaaaaa ccggccatca tccatttgga taagttaaat cgacacggct taatagccgg
      721 agcgaccgga acagggaaga cggtgacttt gaaagtgata gcggaaaaat tgagcaaagc
      781 tggtattccg gtattcctcg cggacgtcaa aggggacctg gcgagcattg cggaaccggg
      841 tgagttgagc gaaaagatac tggagcgagc agcgaaactg caaccggagg attttgagcc
      901 gcgcgcatat cctgttgaac tcctggatat attcggagcg aacggtgtac cggtgcggac
      961 aaccatttcc gatatggggc cgattttgtt gtcgcgcttg ctgggactca atgaaacaca
     1021 agagggtatc ctgaacattg cgttcaaagt atccgatgaa aaacaactct tgctcatcga
     1081 catcaaagat ttgcgggcga tgttgaattt tgtcggagac aacgccacag aattgcgtcg
     1141 tgaatacggg aatatttcca agcaatcgat cggtgcgatc ttaagaggtc tattggttat
     1201 tgaggaacag ggaggcgacc ttttcttcgg ggagccgatg ttcgatatcc aggatttatt
     1261 gaaaacagat gcagctgggc gcggctatgt gaacattctc atggcgaacc gcctgttcat
     1321 gtctcctaaa ctctactcga ctttcctgct atggtttctt tcggagctgt atgagcaatt
     1381 gccggaagtc ggtgaccttg aaaaaccaaa gattgtcttc ttttttgatg aagcgcatgt
     1441 ccttttccag gatgttccgg atgtgcttga ggaaaaaatc gaactcattg tccggctgat
     1501 ccgttcc
//
LOCUS       DAOMUJ010000476         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23200_length_1502_cov_1.685556, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000476 DAOMUJ010000000
VERSION     DAOMUJ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23200_length_1502_cov_1.685556"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLT73_09580"
     CDS             <1..242
                     /locus_tag="PLT73_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20659.1"
                     /translation="LPHKVYDYYNAFLGSKSYMQVYDRYPIAYSVMGHVHLRKMFHEN
                     DTTFSCACLGSSRHWYTEDPYIEMAYTMEEIVVDL"
     gene            387..1340
                     /locus_tag="PLT73_09585"
     CDS             387..1340
                     /locus_tag="PLT73_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRM20660.1"
                     /translation="MRAFNLSDKGVYSQTKNWKSAEWIHVENPDSDDVADLMSTFGIP
                     MDYILDSLDEYEIPRQERVNNGSGDSINLLIVLFPKMRSVQEKFTEYHTYPLSIITVN
                     DTLITICKETPSFLTRIMKNESFKNEPATTQQHIILYILWELTKSFVHHLREIDGTIE
                     DLQIKITSATHNEHFYNLIALHKSLVYFETSINKNHPLITKSIQNASLGDDLQSQELL
                     RDIIDISSQAEVMVTESSKMIDQLSEVFSSVIANNLNNIMKVLTSITIVLTIPTIIAG
                     FWGMNVDLPFENHPWAFGITSLLTIFICFLTVYWLKKKDYF"
BASE COUNT          440 a          297 c          322 g          443 t
ORIGIN      
        1 cgcttccgca taaggtctat gattactaca acgctttcct gggcagcaaa agctatatgc
       61 aagtctatga tcgctatccg attgcttaca gtgtcatggg ccatgtccat ctccgtaaaa
      121 tgttccacga gaacgatact accttctctt gcgcctgtct cggcagttcg cgacactggt
      181 acacggaaga tccttatatc gaaatggctt atactatgga ggaaatcgtc gttgacttgt
      241 agttttccgc tctttctcgg tgggcatttt ttctgaccga ctaagaccgt cccgaaaggg
      301 ttattgcttt cattttgatc aaaatacgtt atgctattcc tatagaatac aacacttaga
      361 ttatagtggg taagaggtgg ttttcaatga gagcgttcaa cttatcggat aaaggggtat
      421 attcacaaac aaagaattgg aaatcggctg aatggataca tgtggagaat ccggacagtg
      481 atgacgtcgc ggatttgatg agtacttttg gcatcccgat ggattatatc ctggattcat
      541 tggatgaata tgaaataccc cggcaagaga gagtcaacaa tgggtccggg gattcgatca
      601 atctgcttat cgtccttttc cctaaaatgc ggtcggttca ggaaaaattt acggaatatc
      661 atacatatcc tttgtcgatc atcacggtca atgacacatt gatcacaatc tgcaaagaga
      721 cgccttcttt tttgacacgc atcatgaaga atgagtcttt caaaaatgaa ccggcgacaa
      781 ctcagcagca tatcatcctg tatattttgt gggaactgac caaaagtttt gtccaccatt
      841 tgagagaaat tgacggtaca atcgaagatc tgcaaataaa aatcaccagt gcgacccaca
      901 atgaacattt ctataatttg attgcccttc acaaaagctt ggtttatttt gaaacgtcaa
      961 tcaacaaaaa tcatcccttg attacgaaat ccattcagaa tgcttcctta ggcgacgatt
     1021 tgcaaagtca ggaattattg cgtgatatca tcgatatctc cagccaggca gaggtgatgg
     1081 tcacagaatc aagtaagatg atcgatcagc tgagtgaagt tttttcgagc gtaatcgcga
     1141 acaatctgaa taacatcatg aaagttttga cttcgatcac cattgtcctg acgattccga
     1201 ctatcatagc cggtttctgg ggcatgaatg ttgatttgcc ttttgaaaat catccttggg
     1261 cgtttggtat cactagcttg cttacgattt tcatctgctt tttaaccgtc tactggttga
     1321 agaagaaaga ctatttttga caggaaaagc aatcaatctt ggctggtata ctaggtatgg
     1381 gtggtgatgc aacatggtag aggacttcca gagttggttg gataacaatg atttagaaaa
     1441 actcttcagt gataaaaggg ataaagaaac aatcgacaag gaaatgtttc ttttcgtccg
     1501 at
//
LOCUS       DAOMUJ010000477         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23224_length_1501_cov_1.603043, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000477 DAOMUJ010000000
VERSION     DAOMUJ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23224_length_1501_cov_1.603043"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLT73_09590"
     CDS             <1..1218
                     /locus_tag="PLT73_09590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRM20661.1"
                     /translation="ETETKPTIIAKPKQATNHNNAMNQSIRVDIEKLDSFMNLVSELV
                     IYRTRLEEINEDLQRSELREPLEQVSRIATDLKELVLKIRMQPLSVVTNRFGRMIRDL
                     SNELEKDIDLVIEGDDTELDKTVVSELGEPLIHLLRNAVDHGVETPEARTAKGKNPNG
                     TIRITAYQEGNRVFLTVSDDGKGVNPTVIKESAGRKGIETDHLSDKEIQQLIFHPGFS
                     TAVNVTNISGRGVGMDVVKQKINELGGTIEIISEVDKGTAFRMSLPLTLSIIQALLVT
                     VGTEQFALPLGVVRKVIRPEPEEIVRTHTGEVYYFEDETIPVVRLQDSLDIHTGVEDK
                     PYLIIVKIDERLYALAVGNLIRQQEIVIKELGKELKTINKYLGATIMGNGNIILILDI
                     TAICNERNVAVYV"
     gene            1211..>1501
                     /locus_tag="PLT73_09595"
     CDS             1211..>1501
                     /locus_tag="PLT73_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRM20662.1"
                     /translation="MYKDYSEMQLDIITEFFSIGVGNAATSLSQLINHPVVMTVPLVE
                     TMDYSELFEGIMSDESSVNAAITMIHGELEGAFLFVIRDDEADKLVSLMLGET"
BASE COUNT          449 a          306 c          400 g          346 t
ORIGIN      
        1 gaaacggaaa caaagccaac aattattgct aagccgaaac aagccacgaa tcataacaac
       61 gcgatgaatc agtcgatacg ggtcgatatc gagaaattgg attcctttat gaatcttgtg
      121 tcggaattgg tcatctatcg gacacgcttg gaagagatca atgaggatct gcaaaggtcc
      181 gaactacggg agccattgga acaagtgagc cggattgcga cggatctcaa ggaattggtg
      241 ctcaaaatcc gcatgcagcc cctcagcgtt gtcacgaatc ggttcggcag aatgatccgt
      301 gacttgagca acgagttgga aaaagatatt gatctggtca tcgaaggcga tgatacagaa
      361 ttggataaga cagttgtatc agaactgggc gagccgctca tccatcttct caggaatgct
      421 gtggaccacg gagtagaaac gcctgaagcc agaacggcaa aaggaaagaa ccctaacgga
      481 acgatccgga taacggctta ccaagaaggc aacagagtgt tcctgacggt atccgatgat
      541 ggcaaaggtg tgaatccgac agtcatcaaa gaaagtgctg gccgaaaagg gatcgaaacc
      601 gaccatttat ccgataagga aatccaacaa ttgattttcc atccaggctt ctccactgca
      661 gtaaacgtga ccaacatttc cggacgggga gttggcatgg acgttgtgaa gcaaaaaatc
      721 aatgaactcg gcggtacgat tgagatcatc agtgaagtcg ataaaggcac cgcattccgg
      781 atgagcttgc cgctgacatt gtcgatcatc caagctttgc ttgtaaccgt tggaacggag
      841 cagtttgcgc tgccgcttgg tgttgtcaga aaagtgatcc gccctgagcc ggaagagatt
      901 gtccggacgc acaccggtga ggtatactat tttgaagacg agacgattcc ggtcgttcgc
      961 ctgcaggata gtttggatat ccataccgga gtggaagata aaccttatct gataatcgta
     1021 aaaattgatg agcggctgta cgctttggca gtgggtaatc tgatccgtca acaggaaatt
     1081 gtcattaaag aattaggcaa agagctgaag actatcaata agtacttggg agccaccatc
     1141 atggggaacg ggaatatcat cttgattctg gatatcaccg caatatgcaa cgaaagaaat
     1201 gtggctgtct atgtataaag attattcaga gatgcaactg gacatcataa ccgagttttt
     1261 ttcgataggc gtagggaatg cagcgacaag tctatcgcag ttgatcaacc accctgtcgt
     1321 aatgacggtg cccttggtgg aaacaatgga ctattccgaa ttgtttgaag ggatcatgtc
     1381 cgatgagagc agcgtgaatg ctgccattac aatgattcat ggagaactgg aaggcgcgtt
     1441 tttgtttgtg atcagggatg atgaagccga caaactggtg tcattgatgc tgggtgagac
     1501 a
//
LOCUS       DAOMUJ010000478         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23261_length_1499_cov_2.073407, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000478 DAOMUJ010000000
VERSION     DAOMUJ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_1499_cov_2.073407"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /gene="cgtA"
                     /locus_tag="PLT73_09600"
     CDS             <1..546
                     /gene="cgtA"
                     /locus_tag="PLT73_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HRM20663.1"
                     /translation="DPFDDYEKINKELESYQLRLLERPTVIVANKMDVAEAEENLEVF
                     KQKIAEKFGEDEAPRIFPISAWSTTGVAELLDYTAEVLKETPEFPLFDEEEVESSVLY
                     KFEENQEQFTIGRDPDSTWVLGGERLEKLFNMTNFAHDESIMRFARQLRQMGVDEKLR
                     AKGAKDGDMVRIMTYHFEFVD"
     gene            585..>1499
                     /locus_tag="PLT73_09605"
     CDS             585..>1499
                     /locus_tag="PLT73_09605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRM20664.1"
                     /translation="MTVQKRSVPLDGMRALAIIAIIFYHLMPHVLPGGYLAVNIFFIL
                     TGFFVTASVIKEYSEKGKLAYKSYLSRRFHKLFFPLLSMMIVVTAYITLFQRGLLLNL
                     RPWILSSLGMFNNWWQISVGASYFEQFYVQSPFMHLWFLSVISQFYIIWPLVMLLLLA
                     LYKDKRIILYTAVLLSLFSALAMGMLYVPGEDASRVYYGTDTRLFSFMIGSALSVVWP
                     LERLDEPVPKQIGRRLTLAGLLSYAVLGVMFSSMLDSQAFTYRGGMFLNSVMIGIAVV
                     LTVHPANFISKILRFKPFQWIGRRSFLLY"
BASE COUNT          394 a          313 c          363 g          429 t
ORIGIN      
        1 gatcctttcg atgactatga aaaaatcaat aaggaattgg aaagttacca attgcgtttg
       61 ctggaaagac cgacagtcat cgtagccaac aaaatggacg tggcggaggc ggaagaaaat
      121 cttgaagtct tcaaacagaa aatcgccgaa aaattcggcg aagacgaagc gccaagaatt
      181 ttcccgattt cagcttggtc aacgaccggc gtagccgaat tgttggacta cactgcagaa
      241 gttctaaaag aaacgccgga gttcccatta ttcgacgaag aagaagtcga atcatccgtc
      301 ctttacaaat tcgaagaaaa tcaagaacaa ttcactatcg gacgcgatcc tgattccaca
      361 tgggttttgg gtggcgaaag actggaaaaa ttattcaaca tgaccaattt tgctcatgac
      421 gaaagcatca tgcgtttcgc gcgtcaactg cgccaaatgg gtgtggatga aaaactgagg
      481 gccaagggag cgaaagacgg cgacatggtc cgcatcatga cttaccattt tgagttcgtc
      541 gattaaaaag taacatcagt atcaataaat gggggataat tatcgtgaca gtacaaaaac
      601 gttccgttcc attggatggg atgcgggcgc ttgcgattat tgccatcatt ttctatcatt
      661 tgatgcctca tgttttgccc ggcggctact tagcagttaa catttttttc atcctgaccg
      721 gatttttcgt tacagcgtcg gtgatcaaag aatacagcga aaagggcaaa ctggcttaca
      781 agagctattt gtccagaagg ttccacaagc ttttctttcc gttgctatcg atgatgatag
      841 tagtgaccgc atatatcact ttgttccaaa gaggactctt actgaattta cggccatgga
      901 tcctttcttc attagggatg ttcaataact ggtggcaaat ttctgttgga gcttcttatt
      961 ttgaacaatt ctatgtgcag tcaccgttca tgcatctctg gtttctttcg gtcataagcc
     1021 aattctatat catctggcca ctggttatgt tgttgctgtt ggcgttgtac aaagataaac
     1081 ggataatcct ctacacagcg gtcttacttt ctttgttttc tgctcttgcg atggggatgt
     1141 tgtacgtacc tggagaggac gcttcacgcg tttattacgg gacggatacg cgtttatttt
     1201 cgtttatgat cggcagcgcg ctttcggttg tctggccgtt ggagaggctg gacgaaccgg
     1261 tgccgaagca gattggtcga agattgacgc tcgccggatt attgagctac gctgttctcg
     1321 gcgtcatgtt cagcagcatg ttggatagtc aggcattcac ttacagaggc gggatgttcc
     1381 tcaacagtgt catgatcggc attgcggttg tcttaacagt acatccggca aatttcattt
     1441 caaaaatact gcggttcaag ccgtttcagt ggatcggcag gcggagtttc cttctgtat
//
LOCUS       DAOMUJ010000479         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23269_length_1499_cov_1.608033, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000479 DAOMUJ010000000
VERSION     DAOMUJ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23269_length_1499_cov_1.608033"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLT73_09610"
     CDS             complement(<1..1158)
                     /locus_tag="PLT73_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRM20665.1"
                     /translation="MTLMYIGHAIISVNSIEDTYNKLLQEKITAYKDSIMSDPEGYLT
                     QASAGQSFDPEMSYYIKIADQSRFVIQNPELQGQSELLLENILMNDQILDAIDSNVDD
                     FSRITIKDEENNPLLLMVFVHKFELQGQSGMLVIGYDLDEFQTYQLNAKSITLLLLTV
                     YLMGTIVYYQHLIRNVAEPLGIMTDIAFKYSRNDFSKRITFESYDEISGLGTAMNKLG
                     KTLQATMLMNREERELLNHLFDSLPSGILYFDQNFQLKLVNGPGQDFLDFWRRVDPDA
                     EAKAMPHQFKEMVQYAFKTTAGSEADLSWSQRHYNVKVTPVIQADLQKTTGVLIVLQD
                     VTNERQLDIIRGDFITNVSHDLRTPLQMIKGYSEAIIDNIAATIPTAHVVSS"
     gene            complement(1218..>1499)
                     /locus_tag="PLT73_09615"
     CDS             complement(1218..>1499)
                     /locus_tag="PLT73_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM20666.1"
                     /translation="LVDEVAINLTPKEFELLLYLSKSPEKIFTREVLLKEVWKYEFFG
                     DLRTVDTHVKRLREKLLKQSKPVSKMIVTVWGMGYKFSPNDDHAANDAQ"
BASE COUNT          400 a          345 c          316 g          438 t
ORIGIN      
        1 ggaagaaacg acatgggctg taggaatcgt agcagcgata ttgtcgataa tggcttcgct
       61 gtagcctttg atcatctgca aaggtgtccg caagtcgtgg gatacattcg tgatgaaatc
      121 tccgcggata atatccaatt gcctttcgtt cgtcacatcc tgcagcacga tcagcacccc
      181 agtggttttt tgcagatccg cttggatgac cggtgtcacc ttgacgttat aatgccgctg
      241 tgaccaactg agatccgctt ccgatccggc tgttgttttg aaagcgtact gcaccatttc
      301 tttgaactga tgcggcatcg ctttagcctc ggcatccggg tcaactcttc gccaaaaatc
      361 cagaaaatct tgccctggtc cgttaactaa tttcaattgg aaattctggt caaagtagag
      421 gattccagaa ggcaacgagt caaacagatg attcaacagt tcgcgttctt ccctgttcat
      481 cagcatcgta gcctgaaggg tctttcccaa cttgttcatg gcagttccta aaccagaaat
      541 ctcgtcataa gattcgaacg taatccgctt agaaaaatca ttccgtgagt atttgaaggc
      601 gatatccgtc ataatcccca acggctcagc aacattccgg atcaggtgct gataatagac
      661 gattgtcccc atcaaatata cggtcaacag cagtagcgtg atactttttg cgttcagttg
      721 gtaggtctga aattcatcta aatcataacc gatgacgagc attccggatt gtccctgcag
      781 ctcaaattta tgaacaaaaa ccatcagaag taaaggattg ttttcctcgt ctttgatggt
      841 gattctgctg aaatcatcta cgttactgtc gattgcatcc agaatttgat cattcattaa
      901 aatattctcc agaagcaatt cggactgccc ttgcagttcc gggttttgta tgacgaaacg
      961 gctctgatct gctattttga tataatagga catttcggga tcaaacgatt gcccagcact
     1021 ggcttgcgtg agataacctt caggatcgga catgatcgag tccttatatg cggtaatctt
     1081 ttcctgcagt agtttattat aggtatcttc gattgagttc acactgatga ttgcgtggcc
     1141 gatatacatc aaagtcatga tggttacagt caaaaaagtc aaaaggaccc atgatttgag
     1201 gaatatgctc gtccttctca ttgtgcatcg tttgccgcat ggtcatcatt cggagagaat
     1261 ttataaccca tcccccaaac ggtgactatc attttcgaaa caggtttaga ttgcttcaac
     1321 agtttctcgc gcaaccgctt gacgtgcgta tccaccgtac gtagatcgcc gaaaaattcg
     1381 tatttccaga cttctttcaa aagaacttcc ctcgtgaaaa ttttttccgg ggactttgaa
     1441 agataaagca gcaactcaaa ttcttttgga gtcaaattga tggccacttc gtctaccaa
//
LOCUS       DAOMUJ010000480         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23705_length_1478_cov_1.949403, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000480 DAOMUJ010000000
VERSION     DAOMUJ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23705_length_1478_cov_1.949403"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1333)
                     /locus_tag="PLT73_09620"
     CDS             complement(59..1333)
                     /locus_tag="PLT73_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRM20667.1"
                     /translation="MARKTKNPPSETDVLLTNYIDQTLGNTGKIGWSSEEVSGMVQEV
                     MKRFLERCLESEMDFHLKNGEIVPREKAEPEAQEAINKRNGTSSKTVLTDNSKVRIDV
                     PRDRLSTFDPISVPKYERHLKGLDDKIISMYARGMSNREISAHMKEIYGVSVSAEFIS
                     RVTDDVLEDVRTWRSRPLQDFYPVVFFDALRVKVKSGNTITPKALYLALAVKADGSRE
                     VLGMWLSDNEGAAYWTSVFNEIRARGCSDILIAVTDGLKGMTKAIETVYPKTMHQTCI
                     VHLLRNSTAFVSYKDLKQVMKELKGIYGADNAEEAKNRLEDFAESDLGKRYSVIKQMW
                     LSQWDQVVPLFNFPPEVRKLIYTTNSIEALNRSIRKVIKTRTMFPTDDSVYKLVYLAI
                     KNATKDWSKSVFRWRQAMLQFVVMFGDRFKQG"
BASE COUNT          353 a          363 c          306 g          456 t
ORIGIN      
        1 gtggaacgta gtggaacaag cgaaccgccc ctccggcgct ttcgctcatc ccaccattct
       61 atccttgctt gaaccgatct ccaaacatta caacgaattg aagcatcgcc tgacgccatc
      121 taaaaactga tttgctccag tctttagtag cgttttttat tgccaggtag acgagtttgt
      181 aaactgagtc atccgtcggg aacattgttc gggttttaat taccttccta atcgaacggt
      241 tcaaggcctc tatgctgttt gtcgtataga ttagcttgcg aacttccggt ggaaaattga
      301 aaaggggaac aacttgatcc cattggctca gccacatctg ctttatgacg ctgtatcgct
      361 taccgaggtc ggattctgcg aagtcctcaa gtctgttttt tgcctcttcg gcattatcgg
      421 ctccgtaaat tcctttgagc tccttcatta cttgcttcag atccttgtag gaaacaaaag
      481 cagtggaatt tctgagcaag tgcacaatgc atgtctgatg cattgtcttt gggtatacgg
      541 tttcaatagc cttggtcatt cctttgaggc cgtctgttac tgcgatcaga atgtcgctgc
      601 agcctcgagc tctgatttca ttaaatacgc ttgtccagta ggcggcgcct tcattgtcgg
      661 agagccacat tccgagaact tctcgggaac cgtccgcttt aactgctaat gctaaataca
      721 aggctttagg cgtaatggtg ttgccgcttt ttacttttac ccgcagtgcg tcaaaaaaga
      781 caaccggata aaagtcctgc aatggacgac tcctccaggt cctgacatcc tccaaaacat
      841 cgtcggttac tctgcttata aactcagcac ttactgagac accgtagatt tctttcatat
      901 gcgcggaaat ttcacgattg ctcatgccac gtgcatacat actaattatt ttgtcatcaa
      961 gacctttcag gtgccgttcg tacttgggaa ctgatatcgg atcaaacgta cttaatcgat
     1021 cgcgggggac gtctatgcgt actttggagt tgtcagttaa aacggtttta ctgcttgtgc
     1081 cgtttcgctt gtttatcgct tcctgagcct caggctcagc cttttctcga ggaacaattt
     1141 ccccgttttt caagtgaaag tccatttcac tttccaggca tctttcaaga aaacgtttca
     1201 taacttcttg aaccatgccg cttacttctt ctgaactcca gccaattttg ccggtgttgc
     1261 ctaacgtttg gtcgatgtaa ttagttaaga gaacgtctgt ctcactgggc ggattcttcg
     1321 tttttcttgc cattttcgat tctccttttc ccttgcctag gagtttaact aacctctaag
     1381 taggagaaaa tttttaaatc aggcaagaca caaaatttga gacactcccg acactcccga
     1441 attcagccaa tgcgattaac taaaaaggcc gagagttc
//
LOCUS       DAOMUJ010000481         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23736_length_1476_cov_2.309641, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000481 DAOMUJ010000000
VERSION     DAOMUJ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23736_length_1476_cov_2.309641"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLT73_09625"
     CDS             <1..226
                     /locus_tag="PLT73_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20668.1"
                     /translation="TSGNANAATSLLLVGFNIGAAFSPYGIVLVERLFRLTSERGIFF
                     LEGALLLGGGILGGGYHFLAKHAETSAEEV"
     gene            398..1201
                     /locus_tag="PLT73_09630"
     CDS             398..1201
                     /locus_tag="PLT73_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM20669.1"
                     /translation="MATHKQAVPPPTVFFGEEAFQPIDHTEVRWLGSGGMFVNTRGTC
                     IMMDPMLKDFDMPLLIDMPIAPENIPHLDAVLITHCDNDHYSVPSLHAMEHVSKAFHG
                     PHYVAELMQAEGFPENGHTIGDSFEIGPAKITLTPADHAWQNEKAKYNYRVFRLEDYC
                     GFWIETPDGIIWAPGDSRLMKEHLEMPQPDVILFDFSDNEWHIGLEGAIQMANTYPEA
                     HLILCHWGTVDAPDLNVFNADPQSLYDRVVNPERIHAYAPGQPFSLKKS"
     gene            1220..>1476
                     /locus_tag="PLT73_09635"
     CDS             1220..>1476
                     /locus_tag="PLT73_09635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004563685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM20670.1"
                     /translation="MKRLYLVRHGETLFNTQGKIQGWSDSPLTERGIKQAQAVRDYFG
                     SEGISFDHAYSSTSERASDTLELITGMPYARLKGLKEVNYG"
BASE COUNT          372 a          361 c          414 g          329 t
ORIGIN      
        1 gacgtcgggg aacgcgaatg ccgccacgtc gttgctgctt gtcggattca atatcggcgc
       61 agccttttcc ccttacggta tcgttctggt ggagcgcctg ttccggctga cttcagaacg
      121 gggaattttt ttcctggaag gggcactgct gctggggggc ggtatcctcg gtggcggcta
      181 tcattttctg gcgaagcatg ctgaaaccag tgcggaggaa gtgtgagcag ctcggtttcg
      241 gcgcaacatt gatctcaatg gagcgctgaa acagggtcct tgctggtcct gcttagcgaa
      301 ttgtgttatt ttggatatgt gaacgcattc attttgagaa aaacaaaaaa actaagactg
      361 catgaaaaag ctagacaact ttaaggagga aaagaaaatg gcaacacaca aacaagccgt
      421 tccaccacct accgtatttt ttggagaaga agcttttcaa ccgatcgacc acacggaggt
      481 ccgctggctg ggcagcggcg ggatgttcgt gaacacccgc ggcacctgca tcatgatgga
      541 tccgatgctg aaggacttcg atatgccgct tctgatcgac atgccgattg cgcctgaaaa
      601 catcccgcat ctggatgctg tgctgattac gcactgcgac aatgaccatt acagcgtgcc
      661 gtcgctgcac gcgatggagc atgtctccaa agcattccac gggccgcatt atgtggccga
      721 actgatgcag gcggaaggat ttccagaaaa cggacatact atcggcgaca gtttcgaaat
      781 cggaccggcc aaaataacgc tgacaccggc ggaccatgcc tggcagaatg aaaaagccaa
      841 atacaattac cgagtattcc ggttggagga ttactgcggc ttttggatcg agacaccgga
      901 tggaatcatc tgggcaccgg gagattcccg gttgatgaag gagcacttgg aaatgccgca
      961 gccggatgtg attctcttcg acttttccga caacgagtgg catatcggat tggaaggggc
     1021 catccaaatg gcgaacacct atcctgaagc acatctgatt ctttgccatt ggggcacggt
     1081 ggatgcgccg gatctgaacg tgttcaacgc cgacccgcaa tccttgtacg accgggtggt
     1141 taatccggaa aggatccatg cttacgcacc gggacagcca ttcagcctga aaaaatccta
     1201 aatgattgga gattcgcgga tgaaacgatt atatttggtc cgtcacggcg agactttgtt
     1261 caatacccaa ggaaagattc agggctggag cgactcgcct ttgacggaac ggggcatcaa
     1321 acaggctcag gccgtgcggg attatttcgg atcggaaggc atcagcttcg atcatgccta
     1381 cagttcgaca tcggaaaggg caagcgatac gctggaacta atcaccggca tgccctatgc
     1441 acgtctgaaa ggcttgaagg aggtcaatta cggctt
//
LOCUS       DAOMUJ010000482         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23867_length_1470_cov_1.654417, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000482 DAOMUJ010000000
VERSION     DAOMUJ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23867_length_1470_cov_1.654417"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLT73_09640"
     CDS             complement(<1..856)
                     /locus_tag="PLT73_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM20671.1"
                     /translation="MLIREKMETIKFSPAEKEVVDYLLRYPEVLDEKTMQEIAAETYT
                     QPSTLIRIAKKLGFAGWVECKKAYQEEHDYLTRNFVDIDANLPFKANDSIMTISKKIA
                     SLGQSTIEDTLSLIHHDTLQEAKQMLLKAKHIQIFATNANMLIPQDFALKMNRIKYHT
                     AVSTIKGEDVYTAYNCPEGTCAILISYTGESNAMKQIANILKAEGIPTIGITSIGDNY
                     LSRAVDCYLPITTREKLYSKIGNFTVNLSVIYLLDVLYSIVFAEKYEENLAHIIRLGK
                     IADKRKTSS"
     gene            1114..>1470
                     /locus_tag="PLT73_09645"
     CDS             1114..>1470
                     /locus_tag="PLT73_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM20672.1"
                     /translation="MTKGVKIVTIGGGSSYTPELMEGFIKRYDELPIREIWLVDIEAG
                     KEKLEIVGTMAQRMWDASPYDVKIHMTLDRREALKDADFVTTQFRVGLLNARVKDERI
                     PFSYGMLGQETNGAGGM"
BASE COUNT          374 a          305 c          372 g          419 t
ORIGIN      
        1 ccgaactggt cttccttttg tcggctattt tgcccaagcg gatgatatgg gccaggttct
       61 cctcgtattt ttcggcaaac acgatcgaat acaggacatc cagcagataa atgacggaaa
      121 ggttgaccgt gaagttgccg atctttgaat atagtttctc gcgcgtcgtg atcggcagat
      181 agcagtctac cgcacgtgaa aggtagttgt cgccgatgct cgtgattccg atcgtgggga
      241 tgccttcagc ttttaatata ttcgcgatct gcttcattgc gttgctttcg ccggtatagg
      301 aaatcaagat ggcgcaggtg ccctcgggac agttgtaggc tgtgtagacg tcttcgcctt
      361 tgatggtaga gacggctgtg tgatacttga tccggttcat cttcaaggca aagtcctgtg
      421 ggatgagcat attggcattg gtggcgaaga tttgaatgtg ctttgccttc agcagcatct
      481 gcttcgcttc ctgcagcgta tcgtggtgga tcagcgagag cgtgtcctca atagttgatt
      541 gtccgagaga ggctatcttt ttactgatcg tcatgatcga gtcgttggct ttgaacggca
      601 ggttggcatc gatgtcgacg aaattgcggg tcagatagtc gtgctcctcc tggtaagcct
      661 ttttgcattc cacccatccg gcaaaaccca attttttggc gatgcggatc agtgtggaag
      721 gctgcgtata ggtttccgct gcgatctcct gcatcgtctt ttcatccaac acttccggat
      781 aacgcagtag gtagtccacg acttcttttt cagccggaga aaacttgatc gtttccattt
      841 tttcgcgtat cagcatgatt tgcatacctc cttaatcagg gctgaacgaa cccgttcagc
      901 ctttctttct atttacaaaa attataaatt aatgtaaatg acatgtaaac gatattatgc
      961 tcaaaaaagg tcaaaaagcg aaaaagattt gaatatttag gcggattttg cgtgaatttt
     1021 gtaaaaaggt tttgaagtgt acccagaacc cttgttatcg gtatttacat gatggataat
     1081 aaccttgtaa acgaaacgag gaggaatata catatgacaa aaggcgttaa aattgtaaca
     1141 atcggcggag gaagcagcta cacacctgaa ttaatggaag gtttcattaa acgttacgat
     1201 gaactgccta tccgcgaaat ttggttagtg gatatcgaag cagggaaaga aaaattagaa
     1261 atcgttggaa caatggcgca acgtatgtgg gatgcttccc cttacgacgt gaagatccat
     1321 atgactttgg accgcagaga agctttgaag gatgctgact tcgtaacgac gcaattccgt
     1381 gtcggtttac tgaatgcccg cgtcaaagat gagcgcattc ctttctccta tggcatgctg
     1441 gggcaagaaa cgaacggcgc cggtggtatg
//
LOCUS       DAOMUJ010000483         1465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_23999_length_1465_cov_1.378014, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000483 DAOMUJ010000000
VERSION     DAOMUJ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23999_length_1465_cov_1.378014"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /gene="dnaE"
                     /locus_tag="PLT73_09650"
     CDS             complement(<1..1452)
                     /gene="dnaE"
                     /locus_tag="PLT73_09650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM20673.1"
                     /translation="MGLLKMDFLGLKNLTILNDAVQLASAACKESINIWEIPMDDNRT
                     LEIFRKADTNGVFQFESPGIKNVLRKLGPESIEDIAAVNALYRPGPMEQIDLFVSRKK
                     GLAAIDYLHPDLKSILEVTYGVMVYQEQVMQVASRMAGFSLGEADILRRAIGKKQKSA
                     IDEERRHFIEGSLQQGYSEQTAEQVYDYIERFANYGFNRSHSVAYSFIAYQLAYMKAH
                     FPEAFFAALLNSVNQHSDKMREYFIELKRRNISISYPDINTSNWKFALQQQTIQFGLG
                     GIKGLRRDFIQEIIKERQAHGHYKDFVQFLRRIHPKWLKNENITPLIFSGAFDSFGHS
                     RATLLQSLPGMLNSIDYSGNNIDLFSILEPKYVETEEMPLLELLDLEEAALGHSLKGH
                     PIDQFGKLYNNGAVAYVTELAYDKKMRTMGLIKDIRRIQTKKGDPMAFANLTDSTGEI
                     SITIFPEYYIRFMKLLKINQLLVVEGKLERSKQA"
BASE COUNT          320 a          376 c          351 g          418 t
ORIGIN      
        1 ggcttgtttc gaccgctcca gcttgccttc gacgacaagc aactggttta tcttcagcag
       61 tttcatgaat cggatatagt actcggggaa aatcgtgatg ctgatttcgc cggtactgtc
      121 tgtcaagttt gcgaaagcca tcggatcgcc cttcttcgtc tggattctgc ggatgtcttt
      181 gatcaagccc atcgtgcgca ttttcttgtc gtatgccagc tcggtgacgt acgcaacagc
      241 accattgttg tataatttgc cgaactggtc gatcggatgg ccttttaaag aatggcccaa
      301 cgcggcttct tccaaatcca gcaattccaa taatggcatt tcttccgttt cgacatattt
      361 cggttccagt atcgaaaaca gatcgatgtt gttcccgctg taatcgatgc tgttcagcat
      421 acccggcagc gactgcaaga gcgtcgcacg cgaatgcccg aaactatcga aagccccgct
      481 gaagatgagc ggtgtgatat tttcgttctt cagccacttc ggatgtatcc ttcttaaaaa
      541 ttgcacgaaa tccttgtagt gcccgtgagc ttggcgctct ttgatgattt cctggatgaa
      601 atcgcgtctg aggcccttga tcccgcccaa gccgaattga atcgtctgct gctgaagggc
      661 aaacttccag ttgcttgtgt tgatgtccgg ataggaaatg ctgatgttcc gccttttcag
      721 ttcgatgaag tattccctca tcttatccga gtgttggttg acggaattca gcaaagcggc
      781 aaaaaatgct tccgggaagt gcgccttcat gtaggcgagt tggtaggcta tgaaggaata
      841 ggccaccgaa tgcgaacggt tgaacccgta attggcgaaa cgctcgatgt aatcgtacac
      901 ttgctcggct gtctgctcgg aatacccttg ctgcagcgaa ccttcaatga agtgtcttct
      961 ttcttcatca atggccgatt tttgtttttt gcctatcgcc cttctgagga tatccgcttc
     1021 ccccaagctg aagccggcca tccgtgaagc cacctgcatg acctgttcct gatagaccat
     1081 taccccgtag gttacctcga ggatcgactt cagatcggga tggagataat cgattgcggc
     1141 cagccctttt ttccgcgaaa cgaaaagatc aatctgttcc atcggaccgg gtcggtaaag
     1201 cgcattgact gcggcaatgt cttcgatcga ttccggcccg agcttgcgca acacattttt
     1261 tatgcctggg gattcgaact ggaacacccc attcgtatcc gcttttcgga atatttcgag
     1321 cgtgcgatta tcatccatcg gtatttccca aatgttgatg ctctctttgc atgccgcgct
     1381 ggccaactgc accgcatcgt tcagtatcgt cagatttttc aacccaagga agtccatttt
     1441 gagaaggccg atttcttcga tgttg
//
LOCUS       DAOMUJ010000484         1454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24183_length_1454_cov_1.551823, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000484 DAOMUJ010000000
VERSION     DAOMUJ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24183_length_1454_cov_1.551823"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLT73_09655"
     CDS             <1..288
                     /locus_tag="PLT73_09655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRM20674.1"
                     /translation="QLFGLSWSTCLNATIEALLKARGAQARSQVQVHVDFCREEDGKG
                     FYFDLKAFVSVEDMELAEVEKLAQSAHKRCPVSKIIGDYNHVTIEVVPFTE"
     gene            391..1019
                     /locus_tag="PLT73_09660"
                     /pseudo
     CDS             391..1019
                     /locus_tag="PLT73_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate:AMP phosphotransferase"
     gene            1152..>1454
                     /locus_tag="PLT73_09665"
     CDS             1152..>1454
                     /locus_tag="PLT73_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20675.1"
                     /translation="MKAAITEEEYEAQLEKLQEEAGQLVYEMWLKKIPCILVFEGTDA
                     AGKGGAISRLTRLIDPRSYDVATTAAPTENESRYNYLWRFYQTFPVKGKMTIYDRSW"
BASE COUNT          445 a          306 c          351 g          352 t
ORIGIN      
        1 caattattcg gcctctcatg gtcgacctgt ttaaacgcaa ccatcgaagc tctgttgaaa
       61 gctagaggag cccaagcacg cagccaggtg caagtgcatg tcgatttctg cagggaagaa
      121 gacggaaaag gcttttattt cgatctgaag gcctttgttt cagtcgaaga catggaattg
      181 gcggaagtcg agaaacttgc gcaatccgcc cataaacgct gtccagtttc aaaaatcatc
      241 ggtgactaca atcatgtcac catcgaagtt gtccctttca cagaatagcg ccttttaaat
      301 cgtctgctgc aattatcaac taatccgtat cactttgcat cagtctgttt tgtgataaaa
      361 tagttaaaag cagaaaaggt ggcgtagcat atgttaaagg actttgattt tgataaaaaa
      421 gacaagacat tatccggcac aaaaaggtct gaactcggca gagaattggc cgctttgcaa
      481 agaaaattgg tcagtagcga ttcatccatg ctgattgtcg tcgatggctg ggaatcctca
      541 ggaaaagggc atatattgaa ggatctgacg agagaattgg atccgcgtta tttcgaagta
      601 agcgcctttg atgatgcctt ggacgattac gcagagcgtc cttttttgtg gcgtttcgca
      661 cgggatttac ccggaaaagg gaggatcgct tttttgaccg gagtttctat tctgaactca
      721 tgaacgatct gaaagtgaag gatgaacgac tggaacatca ccttggctat attagtttct
      781 tggagcggat gctggttgct gataacacga ttgtcctgaa actgtttctg caccagtccg
      841 aaaacacgat ggaaaaacgg atttcgaaac tgaaagaaga tccttacaga gaattcctca
      901 tcacgaaaga cgatgaaaaa caactcaaaa agtaccctaa atatttgaaa catttcgata
      961 aaatactgga aaacacaaat ttcgaatcgt ctccgtggca cgtaatttct tcagaggatc
     1021 tgaaagacgc ttcccgagaa gcgatcagca tcgcaatcag cactttgaaa agtcatcttg
     1081 agcagaccga aaagaaacca ccggctccga tccgatccgg cttgaaggaa aagccactgg
     1141 ccaaagtcga tttgaaagct gccataacgg aagaagagta tgaagcacag ctggagaaac
     1201 tgcaggaaga agcggggcaa ttggtctacg aaatgtggct gaaaaagatt ccgtgtatcc
     1261 ttgtgttcga agggacggat gcagccggca agggtggtgc aatcagccga ttaacgagac
     1321 tgatcgaccc ccgcagctat gatgtcgcca caactgctgc acctacggaa aacgagagcc
     1381 ggtataacta tctttggcgt ttctatcaga ctttcccggt aaaaggaaag atgaccattt
     1441 atgacagaag ctgg
//
LOCUS       DAOMUJ010000485         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24192_length_1453_cov_2.396280, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000485 DAOMUJ010000000
VERSION     DAOMUJ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24192_length_1453_cov_2.396280"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..262)
                     /locus_tag="PLT73_09670"
     CDS             complement(74..262)
                     /locus_tag="PLT73_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM20676.1"
                     /translation="MIALKFDFKPVLSTVMWVLIFMLMAFILFGAGLMVGYGVLGDGN
                     PALVFSKQTWEHIFDYIR"
     gene            complement(344..1315)
                     /gene="mreB"
                     /locus_tag="PLT73_09675"
                     /pseudo
     CDS             complement(344..1315)
                     /gene="mreB"
                     /locus_tag="PLT73_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
BASE COUNT          362 a          394 c          277 g          420 t
ORIGIN      
        1 cgcacctatt caggaaaaaa atcaaaaaag ggcagacgga atgcaatcgt tcctgtctgc
       61 ccttcttatt tccttaacgg atataatcaa agatatgttc ccaggtctgc ttgctgaaga
      121 cgagggccgg attgccgtca cccagaacac cgtatccgac catcaatccg gctccgaaga
      181 gaatgaaagc catcaacatg aatatcaaaa cccacatcac cgttgacagt acaggtttga
      241 aatcaaattt caaagcgatc acctatcttc ctttttttat ttttgttttt tggatcagtc
      301 gcgccaacaa accgaaaagt ccgctggacg gacgattctt ttgatcgatg ttgttcagta
      361 ggatacccgt tcccaaagca actgcatcca atggtttttc agcagcaaat accggaactt
      421 tcaactcgtt cgagaagagt ttctcgattc cgctgatcaa ggcaccccca cctgtcaaca
      481 cgattccggt gttgatgatg tcggcagcca actcaggagg cgtcatttcc aaaacttcct
      541 tcgcttgctc cacgaccaga accaaagtct cgtgcatcgc ttcatatact tccttcgaag
      601 tgatgtaaat agtgcgcggc agtcccgtca ccatgtctcg gccacggatt tccatcttct
      661 cttccttctc aggctccaaa gcggttccga tcgttatttt gattttttca gcagtccgtt
      721 caccgatcaa caatttgtgt tctcttttga cgaaattcat gatgtcggca tccagtttat
      781 caccggctgt tttgatcgag ctgctcgtta cgataccgcc caaagacaat actgcgatat
      841 cactcgttcc gccacccata tcgatgacca tattaccgtt cggctgaaaa atatcaagcc
      901 cggctccgac agcagcaact ttcggttctt cctccagata cacattcttg ccgccgctct
      961 tttctgcggc ttggatgatc gctttttgtt caatcgttgt gatgttcgtc ggacaacaga
     1021 tcaaaatgtt cggcttagtc aaaaaaccct tcacattcaa gcgatcaata aagtgggtca
     1081 gcattgcttc tgtgatgtca aagtctgcga taacgccgcc tttcagcgga cggatgactc
     1141 tgatgtttgc cggcgtacgc ccaaccatca tataggcttc ttccccaact gctaaaactt
     1201 ttccggtttt tgtgtctact gcgacaacgg aaggctcgtt caagacgata cctttcccct
     1261 taacgtgaat cagtacattt gctgtaccta aatcgattcc tatatctctg gccacgttgt
     1321 tcccctctca aacattcatc atttagcgat agctatcttc cggacgacag ttgtccgact
     1381 tattttacga aaagtgcttc aacttccgca tcgctgaaaa catttttttc atcttcggtt
     1441 acacgttcaa cat
//
LOCUS       DAOMUJ010000486         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24300_length_1448_cov_2.582915, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000486 DAOMUJ010000000
VERSION     DAOMUJ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24300_length_1448_cov_2.582915"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1019)
                     /locus_tag="PLT73_09680"
     CDS             complement(207..1019)
                     /locus_tag="PLT73_09680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM20677.1"
                     /translation="MIKMIAIDIDGTLVNGQKVMTQKVKETIQEAMRRGIKIVLCTGR
                     PPAGIKPYADELGFGEHEDYIIAQNGAYILRADTNETVYKKTLTLAEVQEIYEFGKGF
                     SAGTLLVGEHHYYSLEDEVTESMQKDATIVNVDISVLDPKAVTEEMGLMKILYIGNPN
                     EVDVIESAIPDQMRNDFYIVRSQDFLVEVMEKSSNKGTALTKLADHLGIAMDEVMALG
                     DGENDYEMIQVAGLGVVMSNGTDNLKSIANEITLSSEEDGVAHAIERWAFQS"
BASE COUNT          331 a          406 c          305 g          406 t
ORIGIN      
        1 ttggctcgca gagacaagag gttccttatt cttttttccg aagggctaac ccgtgaagct
       61 ggacatcatt ttagatgcat gaaacatgtt gcaaacttgt gaaacaccta cggtgatgca
      121 cttttgagtt gaaaaaattt tataaactcc tatgtgttaa aaaaaggttt ccggaaatca
      181 ctccggaaac ctttttttac attcgtctag ctttggaaag cccatctctc gatggcatgg
      241 gcaacgccat cttcttcact ggataaggtg atttcgttcg cgatgctttt cagattgtcc
      301 gttccattgg acatcacgac tcccagtccg gcaacttgga tcatttcata atcgttctcg
      361 ccatctccta gtgccatcac ttcgtccatc gcgattccca gatgatccgc caacttcgtc
      421 aaagccgtgc ccttgtttga actcttctcc atcacttcta caaggaaatc ttggctgcgc
      481 acgatataga agtcattccg catctgatcg ggaatagctg attcaatgac atccacctcg
      541 ttcggattgc cgatgtaaag gatcttcatc aaacccatct cttcggttac tgctttggga
      601 tccaatacgc tgatgtccac gttgacgatg gtggcatcct tctgcatcga ttccgtcact
      661 tcatcttcca gactgtagta atggtgctcc ccgaccaaca gcgtgccggc agaaaaccct
      721 tttccgaact cgtaaatttc ctgcacctca gcaagcgtca aggtcttttt gtagaccgtt
      781 tcattcgtgt ccgcgcgcag aatataggcg ccattctgtg cgatgatgta gtcctcatgt
      841 tcgccaaatc ccagttcgtc cgcgtaaggc ttgatgcccg caggcggacg gcctgtgcat
      901 agtacgattt tgatacctcg acgcattgcc tcttggatcg tctctttcac tttttgcgtc
      961 atcactttct ggccgttgac caacgtgccg tcgatatcga ttgctatcat ttttatcatc
     1021 agtgaaccca ccccatcatt ctcattcgat gtatttgcct cttcattgta ccaaaaaatg
     1081 cttcaggatg tgacaatctt gcataaagca gaatgaaaaa tgttgcacag aacagcaatc
     1141 aggccggcgc ctgatcacca actccggcca agacctggac cgccggagtt catcgcagat
     1201 tcggcggccc atctccggcg agcacccgct ggccggagat gggctcagtt tcgaacgctc
     1261 tcctccgttc aagccgtcct ctccggagga cagctcccgc ctgtccattc gcaccctaaa
     1321 aacggtcgac tcagatgaga cgaccgcccg gactaccatc atttattctt cgtaagtact
     1381 gtgacggaca aattttccac ccggatgcgg gatagtcctt cgactattct cggttcaccc
     1441 aagtttga
//
LOCUS       DAOMUJ010000487         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24305_length_1448_cov_2.145729, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000487 DAOMUJ010000000
VERSION     DAOMUJ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24305_length_1448_cov_2.145729"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLT73_09685"
     CDS             complement(<1..1285)
                     /locus_tag="PLT73_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20678.1"
                     /translation="MGLRRIRSFETLALKPLRNIHLTSIKIPTGKITAVVGASGSGKS
                     TLVLEMIYSSLEESGAPVVKLDSKPPGKSSTSIVATYLGVFDEVRNVYAQAAAPYLTE
                     SDFSFNARGACPSCVGTGLDGGKVCSACFGSRFRSDVALVKVEGLSIVELLGRNVNHI
                     ALDGAFTFLTKALQIFDALSLTHITLGRSISSLSGGELQRLKLAKFILNNQRAIHSEK
                     ATVILDEPSRGLDDAAVVRLREAMSQYLGECSVIIIEHNPSFIYRCEYIIDLGRALQT
                     KTRNSIVVGQLGEKSFPSLNHVEVFSSLAHIEKDDTWALPQNAQRRQPEADSEGKRYR
                     LLSSLYVEQKNFDLERQFCLGFKTTVKDRNIHFLLSRSEVQDTMRGASNFLFNPFVAY
                     LEKYSKVPASICKSVLADCKGSITYLDDDPWKTLVQ"
BASE COUNT          391 a          390 c          318 g          349 t
ORIGIN      
        1 cctgcaccaa tgttttccac ggatcatcat ccaagtaagt gatgctccct ttgcagtcgg
       61 ctagcacgga cttacagatc gatgccggca ctttcgaata cttttccaaa taggccacga
      121 acgggttgaa cagaaaattg cttgcccccc tcatcgtgtc ctgaacctcc gaacgtgaca
      181 acagaaaatg aatgttcctg tcttttacag tcgtcttgaa gcccaaacaa aactgtcgtt
      241 ccaagtcgaa gtttttttgc tcgacataaa gagatgataa aagccggtag cgcttccctt
      301 ctgaatccgc ttcaggctgt cgccgctgag cattttgagg aagtgcccat gtgtcatcct
      361 tctcaatatg tgccagcgag ctaaacactt ccacatgatt aagacttgga aaactctttt
      421 ctccaagttg gccaaccacg atcgaatttc gtgtctttgt ctggagtgcc cgaccaagat
      481 cgatgatgta ttcacaccga tagataaagc tgggattgtg ctcaataatg ataaccgagc
      541 attctccaag atactgggac atcgcctcac gcaaccggac gacagcagca tcatcaagcc
      601 ccctgcttgg ctcatccaaa ataactgtag ccttttcgct gtgaatcgcc cgttgattgt
      661 tcagtatgaa tttcgcaagt ttcaggcgtt ggagttcgcc accactcagc gaagagattg
      721 accgccctag cgtgatgtga gtaagcgaaa gtgcatcaaa aatctgcagc gctttggtca
      781 aaaaagtgaa tgcgccatca agtgcgatgt gattgacatt gcggccaagc aactcaacga
      841 tgctcaagcc ctcaactttc acaagtgcga catcggaccg aaatctggaa ccaaagcagg
      901 cactgcaaac cttgccgcca tctaaaccag tcccaacaca tgatgggcat gcgcctcttg
      961 cattaaaact gaaatcactc tcggtcagat aaggtgcagc ggcctgcgcg taaacatttc
     1021 gaacttcgtc aaagacgcct agataagtag cgacaatgga ggtcgaagac ttcccgggcg
     1081 gcttgctgtc gagtttgaca acaggcgcac ctgactcctc aagtgacgaa taaatcatct
     1141 ccaaaacaag cgtgctcttg cccgatccgc ttgcaccaac aaccgcagta atttttccgg
     1201 ttggaatctt gatacttgtg agatgtatgt tccgcaaagg cttaagtgcc aaggtctcaa
     1261 acgagcgaat ccgtctcaaa cccaagtatt cgctccggtg gaaatactca ggcatcatag
     1321 ctacagtacg agtgcaatgc tcttcaatgt atccgccttg cgcaccagct cttggcccaa
     1381 ctagaagaac acggtccgcg atctgccgat acaacgtgtt gtgctcgaca acaattacgc
     1441 agttcctc
//
LOCUS       DAOMUJ010000488         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24518_length_1438_cov_2.155459, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000488 DAOMUJ010000000
VERSION     DAOMUJ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24518_length_1438_cov_2.155459"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    389..398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(579..1013)
                     /locus_tag="PLT73_09690"
     CDS             complement(579..1013)
                     /locus_tag="PLT73_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM20679.1"
                     /translation="MITIKDEHILLDQTYNNAEAAIVAAGEFLVGHGLVSPPYIDSML
                     ARQRKVSVYVGNFVALPHGDGEGQPYIEKEGICLIQVPDGVNFGSSKEPQIATILFVV
                     ALKEKQLEALQDIAFFCSDIENVMALSDAKDLRAIQNILQNS"
     gene            complement(1031..>1438)
                     /locus_tag="PLT73_09695"
     CDS             complement(1031..>1438)
                     /locus_tag="PLT73_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM20680.1"
                     /translation="TVALAVENISTEIIADPVKVRDKLMNRYQQAPIGIPNTHFALFH
                     ASHAAVLQPCFCVFDLQTPLEIIGMDKEPMTLTRMLVMLAPDPIEENVAKMLGKISGA
                     IIMNDLGMEIFNSGNEAIIYQLLSALLIEEVKK"
BASE COUNT          315 a          374 c          324 g          415 t
ORIGIN      
        1 ccgggatgaa taatgccgtc aaaactcccc aggcgatcag cgcccctata ttcggcatca
       61 ccatgcttga aagcgtgctg cctaactttt gaacctttgc cttcagcgag gcttgttgtg
      121 tctgttccat tttttgtttc tcccttcaaa ctgtttcttt tgatgtcttt attgtatgcg
      181 cttcctcaat tggtttatag tcgtgctttg gcacgtttat ttgtgccagg acttgataca
      241 tgtatccgga gcgcatcgtt gctgagatct gtttcactct tctccgggga acggaggctt
      301 cgccggagtt cggcccacat tctgcttgtg ttctccgata agcatagctt catcgaagtt
      361 aagtagcagt attaccaggg ttctccggnn nnnnnnnnga gcgcatcgtt gctgagatct
      421 gtttcactct tctccgggga acggaggctt taccggagct gcgccctcat tcctcattcc
      481 ttccttcttc tcctgtcagc aggcgcacaa aaaaagcaag caacattcca ccggagggga
      541 tgttgtttgc tttttgcact gatatttact tgttcctgtt aactgttttg gagaatattc
      601 tggatcgccc gcagatcttt ggcatcgctc aacgccatca cgttttcgat atccgagcag
      661 aaaaaagcga tgtcctgcaa agcttccaat tgtttctcct tcaatgccac cacaaatagg
      721 atagtcgcga tttgcggctc cttgctactg ccgaagttga cgccgtcggg cacctgaatc
      781 aagcagatac cttctttttc aatatagggc tgtccctcgc catccccgtg aggcaaggcg
      841 acaaaattgc cgacatagac actcacttta cgttggcgcg ccagcatcga atcgatgtaa
      901 ggcggcgaca ccaatccgtg ccccaccaag aattcaccgg cggcaacgat tgcggcttcg
      961 gcgttgttgt aggtttggtc caacaatata tgctcatctt tgattgtaat catacggatt
     1021 cctcaattct ttacttcttg acttcttcga tcaggagcgc cgacaacaat tggtagatga
     1081 ttgcttcatt gccggaattg aaaatctcca tccctaaatc gttcatgatg atcgctccac
     1141 tgattttccc cagcatctta gcgacattct cttcgatcgg atcgggcgcc agcatgacca
     1201 gcatcctcgt caaagtcatc ggttctttgt ccatgccgat tatttccagg ggtgtctgca
     1261 gatcaaagac acagaaacaa ggctgaagaa ccgcggcatg cgaggcgtgg aacaaagcaa
     1321 aatgggtgtt tggaatcccg atcggcgctt gctgatagcg gttcatcaat ttatcccgga
     1381 cttttaccgg gtccgcaatg atttcagttg aaatattttc cactgccaaa gcgacggt
//
LOCUS       DAOMUJ010000489         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24538_length_1437_cov_1.997829, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000489 DAOMUJ010000000
VERSION     DAOMUJ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24538_length_1437_cov_1.997829"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="thiI"
                     /locus_tag="PLT73_09700"
     CDS             <1..1010
                     /gene="thiI"
                     /locus_tag="PLT73_09700"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRM20681.1"
                     /translation="GIQNFSPVYLVSHDLNEVKRVLVELLGKMETAGKTFKIATRRSD
                     HSYEHDTTFMNAELGATVLAAFPAISVKMKQPDILVRVDIKTSGIMLSTQTYQGAGGL
                     PVGSSGKGMLMLSGGIDSPVAGYLTMKRGVKIEAIHFHSPPYTSPQALQKAKDLAAKL
                     TKFGGEIQFIEVPFTEIQEEIKEKVPADYLMTITRRMMLRITDAIRAERKGLAIFNGE
                     SLAQVASQTLESMVAINDVTTTPIIRPVATMDKLEIIALAQRIDTFDLSVQPFEDCCT
                     IFAPPAPKTKPHLDKARRFEALLDVEPLVMRAVEGIVVSSITGREDTSSQDEALFSDL
                     L"
     gene            1096..>1437
                     /locus_tag="PLT73_09705"
     CDS             1096..>1437
                     /locus_tag="PLT73_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRM20682.1"
                     /translation="MQVTLKGEIVEVLGTQPVVGEKAPAFSLYNTEDEKVALDDLRGS
                     VVLISVFPDINTSVCDKQTRKFNETAADIEGLTLLSVSRNTKEELVNWCSANGIDMQM
                     LHDDAGEFGESY"
BASE COUNT          391 a          308 c          366 g          372 t
ORIGIN      
        1 tcggtattca aaacttttcg cccgtttatc ttgtttcgca cgatctgaat gaagtgaaac
       61 gggttttggt ggagttgctt ggtaaaatgg agacggcggg aaaaacgttc aaaatagcta
      121 cgcgccgttc cgaccattcc tacgagcatg acactacttt tatgaatgcc gagctgggcg
      181 ctactgtgtt ggctgctttt ccggccattt ctgtcaagat gaaacaaccg gatattcttg
      241 tacgtgtaga catcaaaact agcggcatca tgctcagcac gcagacatac caaggggctg
      301 gcgggttgcc tgtcggttcc agcggcaaag gcatgctgat gctttccgga ggcatcgact
      361 caccggttgc cggttatctt actatgaagc gcggtgtgaa gatcgaggct attcattttc
      421 atagtccgcc gtacacaagt ccccaagcat tacaaaaagc aaaagatttg gctgccaaat
      481 tgactaaatt cggtggtgaa atccagttca tcgaagtgcc gttcactgaa atccaggaag
      541 aaatcaaaga gaaggtgccg gcggattatt tgatgacgat cacacgccgg atgatgctgc
      601 ggatcaccga tgccatcaga gccgaacgca aaggattggc catctttaat ggagaatctt
      661 tggctcaagt cgcttctcag acgctggaaa gtatggttgc catcaatgat gtcacaacga
      721 cgccgatcat tcgtcctgtc gctacgatgg acaaattgga aatcatcgca ttagcgcaaa
      781 gaatcgatac ctttgatttg tctgtgcaac cgttcgaaga ctgctgcact atttttgcac
      841 caccagcgcc aaaaacgaaa ccacacttgg ataaagccag acgttttgaa gcgttgcttg
      901 atgtggaacc actcgttatg cgcgcggttg aagggattgt agtatctagc ataacgggtc
      961 gggaagacac ttccagccaa gatgaggcgc tcttttcgga tttgctttaa tctattttgc
     1021 aagtgcgtat ccttaccata aatcgccact tatctgtata atgaagatat tatcgatcaa
     1081 gggggaatgt ttttcatgca agtaacatta aagggtgaaa ttgttgaagt tttaggaaca
     1141 cagccagttg ttggggagaa ggcgccggct ttttcgttgt acaatacgga agacgaaaag
     1201 gtagctttgg atgatctgcg cggatcggta gtcttgatca gcgtattccc ggatatcaac
     1261 acgagcgttt gcgataaaca aacacgtaag ttcaacgaga cggcagcgga catcgaaggt
     1321 ttgactttgt tgtcggtttc cagaaataca aaagaagaat tggtcaactg gtgttcagca
     1381 aacggcatcg atatgcagat gctgcatgat gatgcaggag aattcggcga gtcctat
//
LOCUS       DAOMUJ010000490         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24545_length_1437_cov_1.547033, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000490 DAOMUJ010000000
VERSION     DAOMUJ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24545_length_1437_cov_1.547033"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..550
                     /locus_tag="PLT73_09710"
     CDS             212..550
                     /locus_tag="PLT73_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRM20683.1"
                     /translation="MGKFSSFVKGIMIGGGIALLLSPKPGKEIRKDLADKADETLDKV
                     MDYTDKAKETAKEAVDQAKEMAQETTDTVKQQFASKAAEVEGKFDAETEAPVTESDEL
                     DPIEEAMKTL"
     gene            complement(625..>1054)
                     /locus_tag="PLT73_09715"
     CDS             complement(625..>1054)
                     /locus_tag="PLT73_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM20684.1"
                     /translation="TCAGSMEEVLAKSDFVSLHVPVTAETTGMINQETLRLMKPNAYL
                     INCARGELINEEALHESLKSGVINGAALDVFQEEPIAQDHPFFELDNLIMTPHNAIIT
                     HEAMDLMGYHAAVGIDDVLNGRQPQWQERLLQTAVAEQVQ"
     assembly_gap    1055..1064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          376 a          332 c          352 g          367 t
ORIGIN      
        1 gtttgagcat ggttcagatc aacaagaaga tgagcgatat ttaggctgat ctgatggtta
       61 tcattttgct taatttttgg cgtgttatat actgaattca taaagaaact ctgattttgt
      121 acacattcaa ataggttttt ggtcaacact cgtttgtctt gatgtccgtt ggcagcgttc
      181 gcgttgccgc atgaagaagg aggaaccacg aatgggaaaa ttttctagtt tcgtcaaagg
      241 gattatgatt ggcggaggaa tcgccctact attgtcacct aagccaggaa aagaaattcg
      301 caaagatttg gccgacaaag ccgatgaaac cttggacaag gtgatggact acacagataa
      361 agcaaaggaa actgcaaaag aagccgtcga tcaagctaaa gaaatggccc aagaaacgac
      421 agatacggtg aagcaacaat tcgcctcgaa ggctgctgaa gttgagggga aattcgatgc
      481 cgaaacggaa gcgcccgtga cagagtcgga tgaactcgat ccaatcgagg aagcaatgaa
      541 aacattgtga gcgtaaacaa atagagtgga aaaggcaccc tgtccggatg gcttttccac
      601 tctatttttc agcttatttc ggctctactg aacctgttcc gccacagcag tttgtaataa
      661 gcgttcctgc cattgcggct gtctgccgtt cagcacatca tcgatcccga cggctgcgtg
      721 gtagcccatc agatccatcg cttcgtgcgt gatgatggcg ttatgcggtg tcatgatcag
      781 gttatccaat tcaaagaatg gatgatcctg cgcgattggc tcctcctgaa agacatccag
      841 ggcagcgccg ttgatgacac cgctcttcaa ggattcatga agcgcctcct cgttgatgag
      901 ttctccgcgg gcacaattga tcaagtaggc gttcggtttc atcaagcgca gtgtttcctg
      961 attgatcatt cctgttgttt ccgctgtgac agggacatgc aaggaaacga aatcggattt
     1021 tgccaaaact tcctccatcg aaccagcgca agtcnnnnnn nnnncacgaa gcctgcatat
     1081 tcatttttca cgagaaattc gtcataagca atcaccttca tgtcgaatcc gtttgtcgct
     1141 ttgcgggcaa ccagcgagcc aatgcgtccc agaccgatca gaccgagtgt cttgcctttc
     1201 agctccgtcc ccttgtgttc gtagcgcgcc ccaattgtca tggcgcactt gttggtccat
     1261 gaaagggatc tgatgtgcga ggctcatgat gattccttct ttctcattat gatggaccag
     1321 caaagaacat gcatagctgt tggctgccgg atcgttccaa acgctccgga atggggcagt
     1381 gccgcgtttt ccggatgcga attgatgtca ttagtatagg acaaccaaat ccacaaa
//
LOCUS       DAOMUJ010000491         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24615_length_1434_cov_1.435098, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000491 DAOMUJ010000000
VERSION     DAOMUJ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_1434_cov_1.435098"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLT73_09720"
     CDS             <1..196
                     /locus_tag="PLT73_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20685.1"
                     /translation="IVRFNSLLAGDLFTAEQKALIQGKLLPSRTGKPSSSRLACPVSA
                     NCSRLCKGIINCFWSNYFWG"
     gene            335..1201
                     /gene="rfbA"
                     /locus_tag="PLT73_09725"
     CDS             335..1201
                     /gene="rfbA"
                     /locus_tag="PLT73_09725"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRM20686.1"
                     /translation="MKGIILAGGSGTRLYPLTKAVSKQLMPIYDKPMIYYPMSTLMLA
                     GIKDILIISTPTDTPRFEQLFGDGAELGLNIEYAVQESPDGLAQAFIIGEKFIGDDSV
                     CLILGDNIYYGSGLAKMLQRAAVKEKGATVFGYHVQDPERFGVVEFDENMRALSIEEK
                     PENPKSSYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNRVYLEKGLLDVEIMGRG
                     FAWLDTGTHESLLEAATFIETIEKRQNLKVACLEEISYRMGYITREQLIELAQPLKKN
                     AYGQYLLRLAKA"
     gene            1300..>1434
                     /locus_tag="PLT73_09730"
     CDS             1300..>1434
                     /locus_tag="PLT73_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRM20687.1"
                     /translation="MNVLDTNLQDVKIIEMPVFGDHRGFFTESYAKEKFIAAGIAIDF
                     I"
BASE COUNT          419 a          296 c          361 g          358 t
ORIGIN      
        1 tatcgtgcgc ttcaactcct tgctggcggg agacctgttt acggccgagc aaaaagcgct
       61 tattcaaggt aaattgctgc cgtcgaggac aggaaagcct tcatccagtc gcctagcctg
      121 tccggtatcg gcaaattgtt caaggctttg caaggggatc atcaattgtt tctggtcgaa
      181 ttatttttgg ggatagccgg caacaaactg ggtgagcgat tcctgaaatt cctgaaacgc
      241 tagcggttat tcagccgttc gatagagcga gaacaccatt attaatgaaa gcaaatacac
      301 taaaaattat caggaaagaa taggagctga agaaatgaaa gggattatct tagctggagg
      361 tagtgggaca cgactatatc cgctgacgaa agcagtctcg aaacaattga tgcccatcta
      421 tgacaaaccg atgatctatt atccgatgtc cacattaatg ttggcgggaa tcaaagacat
      481 tttgattatt tccacgccga ccgatactcc ccgttttgaa caattattcg gtgatggggc
      541 tgaattgggc ttgaatatcg aatacgctgt gcaggagagt cctgatggct tggcacaagc
      601 cttcatcatc ggtgaaaagt tcatcggcga tgattccgtc tgcctgattc tgggcgacaa
      661 catttactac ggcagtggct tggccaaaat gctgcaacgc gcggccgtca aagaaaaagg
      721 cgcaacggtc tttggctacc atgttcagga tccggaacgc tttggtgtgg tggaatttga
      781 tgaaaatatg cgggctcttt cgattgaaga aaaaccggaa aatccgaaga gcagttacgc
      841 tgtaaccggg ctgtatttct atgacaatga tgtggtggaa atcgcgaaga acatcaaacc
      901 atccccacgc ggcgaattgg aaatcacgga tgtcaaccgc gtatacctgg aaaaaggctt
      961 gttggatgtg gaaatcatgg gccgtggctt cgcttggttg gatacgggca cgcatgaatc
     1021 cttattggaa gctgcgactt tcatcgaaac catcgaaaaa cgtcaaaact tgaaagtggc
     1081 ttgcttggaa gaaatttctt accgcatggg ctacatcact cgtgagcagt taatcgaatt
     1141 ggcacaacct ttgaagaaaa atgcctatgg ccagtacctg ttgcgcttgg caaaagctta
     1201 agaaaaacac aacgaaccgt tcaaccttga cagggaaata ggatttttcc gcaaggatgg
     1261 tcagcgaggt tcgatcagta aaaaaagggg taatcgaata tgaatgtttt agacacaaat
     1321 ttgcaagacg taaagattat tgaaatgcca gtcttcggtg accaccgtgg cttctttacg
     1381 gaaagctacg ccaaagaaaa atttatagct gcagggattg cgattgattt catc
//
LOCUS       DAOMUJ010000492         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24728_length_1428_cov_2.415149, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000492 DAOMUJ010000000
VERSION     DAOMUJ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24728_length_1428_cov_2.415149"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLT73_09735"
     CDS             <1..1138
                     /locus_tag="PLT73_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM20688.1"
                     /translation="KTIISEDFEILFPQIQDTGFSDDDSLTNFVSGLESSDITLALTA
                     DGKLELEDNLYYYSIAPNAEKEGTYAVFFLNMSDLFVFEQNLNQILLIIMVIGLVTIA
                     TITYLLVSRITKPLQTLALFAKEIGEGNYQTIDEEFVDLELHELKTTMNETTKKLKLY
                     DADQRTFFQNASHELRTPLQIIKTNAEGIEIGIIDDKKGAIAIKNETDKLGTLVEDIL
                     YLSRLETRSADRLTATNDIRETLAYTVERYASVSDQKNLQVLFDFQKDPVLFHYDERD
                     FERAFQNLISNALRYAKGIIRLGCKEIDNRIMITIYNDGEPISKTDLPHIFDRFYKGN
                     KGVHGIGLSIVQSIVQTYGGRIEVMSSAKGTTFTIILPQQNTSN"
BASE COUNT          409 a          368 c          317 g          334 t
ORIGIN      
        1 aaagacgatc atttccgaag atttcgaaat cctcttcccg cagatccagg acacagggtt
       61 cagcgatgac gattcgttga cgaatttcgt ttccggactg gaaagttccg atatcacact
      121 ggcattgacg gctgatggga aattggagtt ggaggataat ctctattact attccatcgc
      181 ccccaacgcc gaaaaggaag ggacctatgc ggtattcttc ctgaatatgt ccgatctgtt
      241 cgtcttcgag cagaacctga atcagatcct tttgatcatt atggtaatcg ggctggtgac
      301 catcgccacc atcacctacc tgctcgtttc aaggatcacg aaaccgttgc agacgttggc
      361 tttgttcgcc aaagagatcg gtgaagggaa ctatcagacg atcgatgagg aattcgtcga
      421 cttggagctc catgaactga agacgaccat gaacgagacc acaaaaaaac tcaagctgta
      481 cgacgcagat cagcgcacat tcttccagaa cgcttcccat gaattgcgga caccgttaca
      541 gatcatcaag acaaacgcgg aaggtatcga gatcggcatc atcgatgata aaaaaggcgc
      601 tatcgcaatc aagaacgaga ccgacaaact gggcacgctt gttgaggata tcctttacct
      661 gtcgcgactg gaaacccgtt cagcggatcg attgaccgcc accaacgata tccgtgagac
      721 attggcctat accgtcgaac gctacgccag tgtcagtgat caaaaaaatc tgcaagtgct
      781 gttcgacttc cagaaggatc ctgtcctgtt ccattatgat gaacgggatt ttgaacgggc
      841 ctttcagaat cttatctcca acgccctgcg ttacgcaaaa ggcatcatcc gtctgggctg
      901 caaggagata gacaaccgga tcatgatcac catctacaat gatggcgaac cgatttctaa
      961 gactgacttg ccgcatattt tcgaccgttt ctacaaaggc aacaaaggcg tgcacggcat
     1021 cgggctatcc atcgtccaat cgatcgtgca gacctacggc ggacgcatag aagtcatgtc
     1081 ctccgccaaa gggacaactt tcacgatcat actgccgcaa caaaatacat ccaattaaat
     1141 aaaaaaagga ccgaaacgct gaccgccccc ataggctagc gtatcggccc ttttttttgc
     1201 attgggattc ggcgctcttt caactttacg catctttccg ctcactttgc tccatttttg
     1261 caacaactct ctgccctgtt tacggagaat tgtttcccgg atcggcctag aataaggaca
     1321 tcaagagaaa caagaaacga ggttgagcgt tatgagcgaa atatgctacc gcaacgaatt
     1381 caaacacgaa atttcccaaa aaaacaaagt cattctcaca agtcggct
//
LOCUS       DAOMUJ010000493         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24771_length_1426_cov_2.107950, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000493 DAOMUJ010000000
VERSION     DAOMUJ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24771_length_1426_cov_2.107950"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..691)
                     /locus_tag="PLT73_09740"
                     /pseudo
     CDS             complement(1..691)
                     /locus_tag="PLT73_09740"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
     gene            complement(713..1195)
                     /locus_tag="PLT73_09745"
     CDS             complement(713..1195)
                     /locus_tag="PLT73_09745"
                     /inference="COORDINATES: protein motif:HMM:NF022663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HRM20689.1"
                     /translation="MNKSMIDKLHEELEEQHLPCEWRLEWHKPFHTVEIVLLLEVSYP
                     PDNSISDIFGHSNHEDRFIFEDSVLFYDRASSKIKTEEYLTGIPFDRKKGIQGGLAEA
                     VVKNIRLTVGEANTKLRDFLKDEGESSFELHWNELNFMQTIETLKELGRFDETYYRYP
                     "
BASE COUNT          360 a          368 c          260 g          438 t
ORIGIN      
        1 tcttgatcag cgatccctac caaaaggttg ttctctttga cgatgacgtc cgaagcataa
       61 gcattataag caatgttctc tttggagacg cgtgttgcga tttcgtcgat atcgtaggca
      121 tagacagtgt ctgcgcccat tgccttggct gcgatgctca aaacgcccga tccggtacca
      181 acatcgatca ctttttcgcc gccccgcatc actgcttcgt gccggtaccg aaggccatgc
      241 ctggatccaa ttgaatcagc atttcttttt catcttcagg tgtgtattct tcccaataag
      301 gtacaatcgt caaaaagcgg gtcaagcgaa tcggatggaa atattctttc caagagtttt
      361 cccaatcctc ttccttcact tcgttgatct gcagctcatt cctcccgatt tttagatcga
      421 tcttttccag ttccgccagc tgttctttga tgaacaacac agtgtcctga aaacccgcgt
      481 tgttcggata ataagccatg atataaacgg catgcccttc ttcaggcaac gcacgttcct
      541 cctttatttg cccgaaaccg tcgtcctgaa gattgacgaa gtccaattcg tcatctatgg
      601 cgacaccttt cgcaccagct tccatcaata tattcgatac agcctcaact gcttcggtag
      661 cagtcacaac cttcacttca ttccattcca tttacattca cctttcctac catcaaggat
      721 aacgataata cgtttcatca aatcttccta attccttcag tgtttcgatt gtctgcataa
      781 aattcagctc attccaatgc aattcgaagc tggactcacc ctcgtctttc agaaaatccc
      841 tgagtttagt gtttgcttct ccgaccgtca agcgaatatt cttaacgacc gcttccgcca
      901 atcctccctg aatccctttt ttccgatcga atgggatacc cgttaaatac tcctccgttt
      961 tgatttttga agacgcgcga tcataaaaca aaacagaatc ttcaaaaatg aaccgatctt
     1021 cgtgattcga gtggccaaat atgtcagaaa tactattgtc tggaggataa ctgacttcta
     1081 ataataacac aatttcgact gtatggaacg gtttatgcca ctccaatcgc cattcacaag
     1141 gcaagtgttg ttcttccagt tcctcgtgga gtttgtcgat catacttttg ttcattctct
     1201 tcctcctcgt gccctcattt cggctgatct ttactggctc tgtgccgcct ccggatgatg
     1261 ctctccatcc attgtagcaa ataatgagaa aaagatatat tccgggctat gcatcagcaa
     1321 attattttag tgaaaatcag ccccggatta ttgagggaac gatttctaaa agatccggtt
     1381 tatgatatat tagtctaggc aaaaagttgg agggatattt catgac
//
LOCUS       DAOMUJ010000494         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24885_length_1421_cov_1.912884, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000494 DAOMUJ010000000
VERSION     DAOMUJ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24885_length_1421_cov_1.912884"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1421
                     /locus_tag="PLT73_09750"
     CDS             81..>1421
                     /locus_tag="PLT73_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3744 domain-containing protein"
                     /protein_id="HRM20690.1"
                     /translation="MRRPLISFQNFSFTYSDQNEPTLEELNVTIYEGEKILVLGPSGS
                     GKTTFARCLTGVLPSEENGDVSGNILFHHQVDQTVAPFEDFLPNEMKLIKNSMIPHAD
                     QMGKFGVKQQIPFRSAEDLKKLENWHEFLQKRPYLKRSFMKLSEQQLGDITMAGISLD
                     GKPLILFDEPLANLDPSSGDLAMKFIDDLHRVSDTTVLIIEHRLEDVLAQPIDRILLF
                     SGGRIIADATPEEILRTEILSDIGIREPLYITAMRYAGVDLDNVRELANVKKVNGPQL
                     KEQMESWLNYLPQFRYADYDEVLLELDGLSFKYPWNDEDIVDGAFLRIYKGEMLSLVG
                     ANGAGKSTLSRLMTGEEKPQSGRIYWKGHEISDIDADEAENYVGYVPQNPKDSLSQET
                     VYAEIALKLRQRNMDEEAIEKKVDEVAKVCGLQYIKDLPLSMLSFGQLKRVSIAA"
BASE COUNT          418 a          292 c          362 g          349 t
ORIGIN      
        1 gaaggctggg tgtgaacatt cagctctttt ttggatagca cttaattatt ttttgaatat
       61 actaatgaag gaggatagcc atgcgaagac cactgatatc ttttcagaac tttagtttca
      121 cttattcaga tcaaaacgaa ccgactttgg aagaactgaa tgtcactatc tacgaagggg
      181 aaaaaatact tgtgctcggc cctagcggat cagggaaaac gacctttgcc cgctgcctta
      241 caggcgtatt gccgagtgaa gagaacggcg atgtgagtgg aaatatcctc tttcaccatc
      301 aggttgacca aacagtagcc ccttttgaag attttcttcc gaatgaaatg aagctgataa
      361 agaatagcat gattccccac gcagatcaga tgggaaaatt tggagtgaaa caacaaattc
      421 cattccgttc cgctgaagat ttgaaaaaac tggagaattg gcacgaattt ttgcagaaac
      481 ggccttatct gaagcgcagt ttcatgaaat tgtcggaaca gcaattgggt gacatcacaa
      541 tggcgggtat ctccttggat ggcaaaccat tgattttatt tgatgagcca ttagcgaatc
      601 tggacccgag ttccggggat ttggccatga aattcatcga cgatctgcat cgcgtcagcg
      661 atacgacggt cctgatcatc gaacataggc ttgaagatgt tttggcacaa ccaatcgacc
      721 gcatcctgtt gttttccggt ggacggatca tcgctgacgc aacgccggaa gagatactcc
      781 gtaccgaaat tttgagcgat atcggcatca gggagccgct gtacatcacg gcgatgcgct
      841 atgccggagt cgatctggac aatgtcaggg agctggcgaa tgtaaagaaa gtgaacggac
      901 cgcaattaaa ggaacagatg gagagttggc tgaattattt gccgcaattc cgttatgccg
      961 actatgatga agttcttttg gaactggatg gtttatcgtt caaatatcct tggaatgacg
     1021 aggacatcgt cgatggagcc ttcctccgaa tttacaaagg ggaaatgctc agccttgtcg
     1081 gtgcgaacgg cgccggaaaa tcgacgcttt cccgtttgat gaccggagaa gaaaaaccac
     1141 aaagtggccg gatctattgg aaaggccatg aaatcagcga cattgatgcg gatgaggctg
     1201 aaaattacgt aggttatgtc ccgcagaatc cgaaagacag tttgtcacag gaaaccgtat
     1261 atgcagaaat tgccttaaaa ctgagacagc gcaacatgga cgaggaagcg atcgaaaaga
     1321 aagttgatga agttgcgaaa gtctgcggat tgcaatacat aaaagactta ccgttatcca
     1381 tgctgagttt cggccaactg aaacgggtgt cgattgcagc c
//
LOCUS       DAOMUJ010000495         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24888_length_1421_cov_1.789165, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000495 DAOMUJ010000000
VERSION     DAOMUJ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24888_length_1421_cov_1.789165"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLT73_09755"
     CDS             <1..348
                     /locus_tag="PLT73_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRM20691.1"
                     /translation="WTAMIRQPEFVTDVVFRWAVEGLKKKKPSLDLAKARLAEFTEGL
                     CVQMMHMGPYDTEAVTVKAIDDFAAANGYVNAISEMSPDGTILRHHEIYLNDPRKVAP
                     EKMKTVVRHPIRK"
     gene            507..1331
                     /locus_tag="PLT73_09760"
     CDS             507..1331
                     /locus_tag="PLT73_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRM20692.1"
                     /translation="MTTWLITGCSSGIGRGIAKAVLKHGDNAIVTARNISTVADIVKN
                     YPDTAIAVPLDITNIESIADAVKAAADNFGGVDVLVNNAGYGYRSSIEEGEEESVNLL
                     FDTNFFGPIELIKQVLPYMRAQKKGAIINISSIAAARSGVGSGYYPASKAALELMTDG
                     LMKEVAPLGIKVMTVEPGAFRTKFYDTSLKGTQKQIEDYADTAWKTRKENIVDNQDQP
                     GDPDKAGEVIYETIQKENIPKRLLLGSDAVRIVSAEMKERLQEIEDWSAVSAQTDY"
BASE COUNT          414 a          301 c          384 g          322 t
ORIGIN      
        1 tggacagcga tgatccgtca gccggaattt gtcactgatg tagtattccg atgggccgtt
       61 gagggattga agaaaaagaa accgtcgctt gacctcgcca aggcaaggtt ggcggagttc
      121 acagaagggc tttgcgttca gatgatgcat atgggtccct acgatacgga agcagtgaca
      181 gtcaaggcta tcgacgattt tgctgcagcc aatggatacg tcaatgccat ttcggaaatg
      241 agtccagacg gtacaatcct taggcatcat gaaatttacc tgaacgatcc gcgtaaagta
      301 gctccggaaa agatgaagac ggtcgtccgc catccgatca ggaaataaaa atagaaaatg
      361 tggaaatgta attttcaaaa ataaaacgct tcccttgaag tgcactccaa ggtgtatact
      421 tctgtttgtg atgcgagtgg cgccattttg attcgcaaaa aggcgcaatg atcaaaataa
      481 acagcttcag gaaggaagga aacattatga caacatggtt gattacaggc tgctccagtg
      541 gtatcggcag aggaatcgca aaagcagttt taaaacatgg ggataacgcg atagtgactg
      601 cacggaacat ttccacggtg gcggatatcg tcaaaaatta tcccgacact gctatcgcgg
      661 tgcctctgga tatcacaaat atagaaagta tcgcggacgc cgtaaaagcg gcagcggata
      721 attttggtgg ggtggatgtc ctcgtgaaca acgctggtta tggttaccgt tcttccatcg
      781 aagaaggcga agaagaaagc gtgaatctgc tgttcgacac gaacttcttc ggcccgattg
      841 aattgatcaa gcaagtcttg ccatacatgc gggcgcagaa gaaaggcgcc atcatcaata
      901 tttcctcgat cgctgccgcc cgctcaggtg tcggttccgg ttattatcct gcatccaagg
      961 ccgctttgga attgatgact gacggcttga tgaaggaagt cgctccgttg ggaatcaaag
     1021 tgatgaccgt ggaaccaggc gcattccgga ccaaattcta cgacacttcc ctgaaaggta
     1081 cgcagaagca gatcgaagat tatgccgata ccgcttggaa aactcgaaaa gagaacatcg
     1141 ttgacaacca ggaccagccc ggcgatccgg acaaagcggg agaagtcatc tacgaaacta
     1201 tccaaaaaga gaacattccg aaacggttgt tgctgggttc ggatgcagtg aggattgtgt
     1261 ccgccgagat gaaggaacga ttgcaggaaa tcgaggattg gagcgcagtc agcgcacaga
     1321 ctgattactg atcggatttt tggaaatgtc aaaaaataaa tgatgaaaga ctgtggacgc
     1381 tggcgggatg gctgttccgc ggtctttttt cggtcgataa g
//
LOCUS       DAOMUJ010000496         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24919_length_1420_cov_1.668864, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000496 DAOMUJ010000000
VERSION     DAOMUJ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_1420_cov_1.668864"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..873
                     /locus_tag="PLT73_09765"
     CDS             364..873
                     /locus_tag="PLT73_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM20693.1"
                     /translation="MECGTELEAKHLENEGNIPYCNSCEGYRFPVFNTAVSMVILNKE
                     QNKILLIKQYGGADYILCAGYVNKGENAEQAAAREIAEELGLQTVGLRYNKSEYFEKN
                     NTLMLNFSCIVASERLDGMTDEVDQAEWFSIEAAKANIKKGSLAQRFLENYLEKSNGL
                     KLVTALKAF"
     gene            complement(971..>1420)
                     /locus_tag="PLT73_09770"
     CDS             complement(971..>1420)
                     /locus_tag="PLT73_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRM20694.1"
                     /translation="LKQLGKNAIFEVSISDGKTMQVIVKDIQQAALKNQIQNVALQAL
                     TKGQKLTMTVAIHLVGSEDRKEGVLTQTLNELEIETDAAHVPTEFQIAVNEMNIGDTL
                     TVADIKVDAGISVLTDPEAAVAILAAPATETADEDEASAEATEKVAE"
BASE COUNT          400 a          283 c          346 g          391 t
ORIGIN      
        1 tgcccgatga atgagcctgc tccggtagaa tcggaagcgg actgcattgc cggtaccgca
       61 ttgatggtgc aatttggtgt tgtgaaagag agcgcgtcgg cagaaaagca ctgcccgacc
      121 tcccaaatca tcaaataata taaatgaaca gcaaaaaaag tcggtgactc aatcgttttt
      181 gattgggtca ccgacttttt atctggagaa aagagtggaa tctcatcctc catcaacaga
      241 tttcaatcaa aaagatggca tgttataata ttccatattg tcctaagctt tttgttgttg
      301 ataaaggctt catgaggaaa cgaacggaat cggaaaggac ttacaaaata gatgaattat
      361 tgcatggaat gcggaacaga actggaagcg aaacatctgg aaaatgaagg gaacatccct
      421 tattgcaaca gttgcgaggg atatcgattc cctgtgttca acacggctgt cagcatggtc
      481 attttgaaca aggaacagaa taaaattttg ttgatcaagc agtacggtgg ggcagattat
      541 attctctgtg cgggatacgt caataaaggt gaaaatgcgg agcaggcagc ggctcgtgaa
      601 atcgccgaag aactcggact acaaacggtc ggattgcgct acaacaaaag cgaatatttt
      661 gaaaaaaaca acacactgat gctgaatttt tcctgtatcg tcgcgagcga acgcctcgat
      721 ggcatgacgg atgaagtgga tcaggcggaa tggttttcca tcgaagctgc aaaggccaat
      781 atcaaaaaag gcagtttggc tcagcgcttc ctggaaaact atttggaaaa aagtaatgga
      841 ctgaagttgg tcaccgcttt gaaggccttc tgataaggat agtccagcaa ataatttgaa
      901 acaaaaaaag attcggcttt gacgttcaaa gccgaatctt tttgtctata cgtaaatttt
      961 gatttggtga ttattcagcg actttttcag ttgcttctgc tgaagcttcg tcctcgtctg
     1021 ctgtttctgt ggcaggcgct gccaagatgg cgactgctgc ttctggatcc gtaaggaccg
     1081 aaataccggc atcgactttg atatcggcaa ccgtcagggt atcaccgatg ttcatttcat
     1141 taacagcgat ttggaattcg gtagggacat gggcagcgtc cgtttcaatc tccaattcgt
     1201 tcaaagtttg ggtcaggacg ccttctttac ggtcttcgct gccgactaaa tggatcgcaa
     1261 cggtcatcgt caatttttga ccttttgtaa gagcctgtag tgcaacgttt tggatttggt
     1321 tcttgagggc agcttgttgg atatccttca cgatgacttg catcgttttt ccatcggata
     1381 tgctgacttc gaagatcgca tttttaccta attgcttgag
//
LOCUS       DAOMUJ010000497         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24920_length_1420_cov_1.638095, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000497 DAOMUJ010000000
VERSION     DAOMUJ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24920_length_1420_cov_1.638095"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..806)
                     /locus_tag="PLT73_09775"
     CDS             complement(57..806)
                     /locus_tag="PLT73_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRM20695.1"
                     /translation="MSGLIYRRIHRIKGYECDITGEITLPAIVNLMMDLSGQQSDSLG
                     NTNDQMSERGLSWIIVQYDMQIERMPHQGEEIILETEAMSYNRFFTYRRFRAYDEREQ
                     LCVEVMTNFVMMDIETRKMVQIQKDLLTVYQADEIRTMVRMQKPIQLEPENRKEQDFR
                     VRYLDIDYNRHVNNAKYFDWIINTIDPKFIMDHQMAAVTIKYEKEVEYGNSITSVMST
                     KEAENGEIITAHRIMNGEDLACEAHMIWKKV"
     gene            complement(887..>1420)
                     /gene="fetB"
                     /locus_tag="PLT73_09780"
     CDS             complement(887..>1420)
                     /gene="fetB"
                     /locus_tag="PLT73_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HRM20696.1"
                     /translation="ASWNAHKRSSSIPNSLKISLIAISASAILTLSILVLSGSVKFIP
                     SQIVPITGMIAGNAMTTIGLCYRNLNSLFRDQRQQILEKLSLGANPNQASRTILRETI
                     KSGMQPSLDSAKTVGIVSLPGMMSGLMFAGTVPMTAIMYQIMVTFMLVAATSISSYIA
                     SFLAYREFYNDRQQLRV"
BASE COUNT          332 a          343 c          346 g          399 t
ORIGIN      
        1 accgattagg tcacagtgtt tccttactca gggctgaacg aacccgttcc gcttttctaa
       61 acttttttcc agatcatgtg ggcttcgcag gctaggtctt cgccgttcat gatgcggtgc
      121 gcggtgatga tttcgccgtt ctcagcttct tttgtcgaca tgacgctggt gatgctgttg
      181 ccgtattcga cttctttttc atacttgatc gtaacggcag ccatctgatg atccatgata
      241 aatttcgggt cgattgtgtt gatgatccaa tcgaaatatt tcgcgttgtt gacatggcga
      301 ttgtagtcga tatccaggta tctgacgcgg aaatcctgct ccttacggtt ttccggttcc
      361 agctggatcg gtttctgcat gcgcaccatc gtgcggattt catcagcctg gtaaacggtc
      421 agcaagtctt tttggatctg gaccatctta cgggtttcga tgtccatcat gacgaagttc
      481 gtcatcactt ccacacacaa ttgttccctt tcatcataag cccggaagcg gcgataggtg
      541 aagaagcggt tataactcat cgcttccgtt tccaggatga tttcttcgcc ttggtgaggc
      601 atgcgttcga tctgcatgtc gtactgcacg atgatccaac tgagtcctct ttcggacatc
      661 tggtcattcg tgttcccgag tgaatcgctt tgttgtcccg acaagtccat catcaggttt
      721 acgatcgctg gcaaggtgat ttcaccggtg atgtcgcatt catagccttt gatgcgatgg
      781 attctgcgat agattaatcc gctcaatgtt ttccctccaa aatcaacttt ataatagaaa
      841 ggataccata cttttcagca cggaccaacc gaaataccat tcaaaatcag acccgcaatt
      901 gctgtctgtc gttgtagaat tcgcggtatg ccaggaaaga ggcgatataa gacgagatgc
      961 ttgtcgccgc caccagcatg aaagtcacca tgatctgata catgattgcc gtcatcggta
     1021 cggtgccggc aaacatcaaa cccgacatca tgcccggcaa ggaaacgata cccactgttt
     1081 tggcggaatc caaagatggc tgcatgcctg atttgatcgt ttcccgcaag attgtgcgcg
     1141 aagcttgatt cggattcgca ccgagagaca gtttttccag aatctgttgg cgctgatcgc
     1201 ggaacaagga attcaggtta cgatagcaca agccgatggt cgtcatcgca ttcccggcta
     1261 tcattccagt gatcggcaca atctgtgaag ggataaattt gacggacccg gacaggacca
     1321 aaatggataa cgtcaaaatc gctgaagcag agatggcgat gagcgaaatc ttcaaagaat
     1381 tggggatgct gctgctgcgt ttgtgggcat tccatgatgc
//
LOCUS       DAOMUJ010000498         1419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24936_length_1419_cov_1.379765, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000498 DAOMUJ010000000
VERSION     DAOMUJ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24936_length_1419_cov_1.379765"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1135)
                     /locus_tag="PLT73_09785"
     CDS             complement(119..1135)
                     /locus_tag="PLT73_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20697.1"
                     /translation="MAYIEFRNIRKSYDGENLVLKNLDLQVNEGDLVTLLGPSGCGKS
                     TLLRSLAGFESIDGGSIHINGQDVTELPPGKRNIGMVFQQYSLFPNMNVAENIAFGLK
                     MQKMDPGLIKEKVAHMIELVGLSGKENHYPTQLSGGQKQRVALARAIVTEPKVLLLDE
                     PLSAIDALLRKNLQKQIRRIQKALHITTIFVTHDQDEAMLMSDVIHVVAAGKIEQSGT
                     PTEIYTHPKTHFVASFIGNYNLLSGSEFEKLTRIKTQASQIAIRPEAIEYFKEPQPET
                     NEHLYFKGKVIDETISGNILSYVIETEQGVTLRADHLYRTFNLLDKGELVFMKVEKRN
                     ILEF"
     gene            complement(1171..>1419)
                     /locus_tag="PLT73_09790"
     CDS             complement(1171..>1419)
                     /locus_tag="PLT73_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20698.1"
                     /translation="GLVATILLSIGILFSDFVLVNIIAGSYYETTSIFLDKTRSVSGH
                     AASAISVVIFCIMLILTNLAGYLGSKETKKAKTNTKME"
BASE COUNT          356 a          364 c          292 g          407 t
ORIGIN      
        1 cctatctgat catcatgggt ggattcatcg gttggctggt cacgatgttt tgaatgtatc
       61 atccagacaa aaagcccctc atcacttgcc ttattccggc ggtgatgagg ggctcttttt
      121 aaaactccaa aatatttcgc ttctctactt tcataaaaac gagctcgcct ttatcaagca
      181 gattgaaggt gcggtacaga tgatccgctc gcagcgtaac gccctgctcg gtttcgatca
      241 cgtatgacaa aatattcccg ctgatcgttt catcgatgac tttccctttg aaatacaggt
      301 gttcattcgt ctcaggctgc ggttccttga aatactcgat ggcctccgga cggatggcga
      361 tttggctagc ctgcgttttt atccgggtca gcttttcaaa ctccgaaccg ctcaacaaat
      421 tgtagttgcc gatgaacgaa gctacgaaat gcgttttggg atgcgtatat atttcagtcg
      481 gtgtgccgga ctgctcgatt tttccggcag ccacgacatg aatgacatcc gacatcagca
      541 tcgcttcatc ttgatcatgc gtcacaaaaa tcgtcgtaat atgcaaagcc ttctggatgc
      601 gacggatctg cttttggaga ttcttccgca acaacgcatc aatagcagac agcggctcat
      661 ccagcaacaa tactttcggt tcagtcacga tggcgcgtgc caaagctacc cgttgcttct
      721 ggccaccgga caattgcgtc ggataatggt tctctttacc ggacaagcca acgagctcga
      781 tcatgtgggc gactttttct ttgatcagac ccggatccat tttctgcatc ttcaaaccga
      841 atgcgatgtt ctccgctaca ttcatgttag ggaacagcga gtattgttga aagaccattc
      901 cgatattccg tttccccggc ggtaactccg tcacatcctg accgttgatg tggatgctcc
      961 ccccatcgat actttcaaaa ccggcaagcg aacgcaacaa agttgatttt ccgcaaccgg
     1021 atgggcccaa caacgtgacc aagtcgcctt cattcacctg cagatcaaga tttttcaaca
     1081 ctaaattttc accgtcatat gattttcgaa tgtttctgaa ttctatataa gccattattt
     1141 ctcctctata cgtaacaaaa cgtgaagcta tcactccatt tttgtatttg tttttgcttt
     1201 tttggtctct ttcgatccca gataacccgc caaattcgtc aaaatcagca tgatgcagaa
     1261 gatgactact gaaatagcgc tggctgcatg tcccgataca cttctggtct tatccaggaa
     1321 aatgctggtt gtttcatagt aacttccagc aatgatgttc accaatacga aatcgctgaa
     1381 caaaatgcct atcgagagca aaatcgtcgc aaccagtcc
//
LOCUS       DAOMUJ010000499         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24953_length_1418_cov_1.900220, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000499 DAOMUJ010000000
VERSION     DAOMUJ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24953_length_1418_cov_1.900220"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1418)
                     /gene="nrdE"
                     /locus_tag="PLT73_09795"
     CDS             complement(<1..>1418)
                     /gene="nrdE"
                     /locus_tag="PLT73_09795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HRM20699.1"
                     /translation="DDVTYYKLNNKINRPIDNAIPLHKDKEAVRAYFLEHVNPNTVFF
                     YTLDEKLNYLIEHDYIEKEFLEKYPLDFIKKLMQDTYNKKFRFKSFMAAYKFYTQYAL
                     KTNDGKRYLERYEDRIVFNALFLADGDQQLAVDLAEEMIHQRYQPATPTFLNAGRKRR
                     GELVSCFLIQTTDDMNSIGRTINSALQLSRIGGGVGVNLSNVRAAGDPIKKIENASSG
                     VVPIMKLLEDSFSYSNQLGQRNGAGAVYLSVFHPDIVAFLSTKKENADEKIRVKTLSL
                     GLVIPDKFYELAAKDDQMYLFSPYDVERIYGKPFSYVDITAEYENLVNNPEISKSKIR
                     ARDLENEISKLQQESGYPYIINIDTANRTNPVDGTIVMSNLCSEILQVQQPSLLNDKQ
                     EYEVLGTDISCNLGSTNIPNLMQSPDFGKSVETMVRALTYVTDHSSIDAVPTVKNGND
                     LAHTIGLGGMGLHTMFATNQMH"
BASE COUNT          320 a          354 c          348 g          396 t
ORIGIN      
        1 taatgcatct gattcgttgc gaacatcgta tgcaagccca tgccgcccaa accgatcgtg
       61 tgcgctaggt cgttcccgtt cttgactgtc gggaccgcat cgatgctcga gtggtcggtt
      121 acataagtca gggcacggac catcgtttcg accgattttc cgaaatcagg cgattgcatc
      181 aggttaggga tattcgtcga acccaagtta cagctgatat cggttccaag cacttcatac
      241 tcctgcttgt cattcagcaa ggaaggttgt tgcacctgca gaatctcgga gcacagattg
      301 ctcatgacga tcgttccgtc taccggattg gtgcggtttg ccgtatcgat gttgatgatg
      361 tacggatagc cggattcctg ctgcagtttg ctgatctcgt tctcaaggtc gcgggcacgg
      421 atcttcgatt ttgaaatttc cggattgttg accaaatttt cgtattccgc ggtgatatcg
      481 acataggaaa acggtttgcc gtagatccgc tccacatcat atgggctgaa cagatacatc
      541 tgatcgtcct tcgccgccag ctcatagaat ttgtccggaa tgacgagacc caacgacaaa
      601 gtcttcacgc ggattttttc atccgcattc tcttttttag tggaaagaaa ggccacgata
      661 tccgggtgga atacgctcag gtacaccgca ccggctccat tgcgctggcc caactggttg
      721 gagtagctga agctgtcctc gagaagcttc atgatcggca cgacgccgct cgatgcgttc
      781 tcgatttttt tgatcggatc cccggctgcc cggacgttag acaggttgac cccgacgcct
      841 ccgccgatgc gggataactg cagcgcgctg ttgatcgtgc ggccgatgct gttcatgtca
      901 tccgtcgttt ggatcaggaa gcacgacacc aattccccgc ggcgttttct gcctgcattc
      961 aaaaaggtag gcgtcgctgg ttggtagcgt tgatggatca tctcttccgc cagatcgaca
     1021 gccaattgct ggtccccgtc agccaaaaag agtgcattga agacgatccg gtcttcataa
     1081 cgctccaaat aacgtttgcc gtcgttcgtc ttcagcgcgt actgggtata gaacttgtag
     1141 gccgccatga aagacttgaa acggaatttt ttgttgtacg tatcctgcat caactttttg
     1201 atgaaatcaa gcgggtattt ctccaggaat tctttttcga tgtaatcgtg ctcgatcaaa
     1261 taattcagtt tttcatccaa cgtatagaag aataccgtgt tcggattcac atgctccagg
     1321 aaataagctc gtacggcttc cttatcctta tgcaaaggga tggcattatc aatcggacga
     1381 ttgattttat tgttcaattt ataataggtt acatcatc
//
LOCUS       DAOMUJ010000500         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24990_length_1416_cov_2.026451, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000500 DAOMUJ010000000
VERSION     DAOMUJ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24990_length_1416_cov_2.026451"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="manA"
                     /locus_tag="PLT73_09800"
     CDS             complement(<1..832)
                     /gene="manA"
                     /locus_tag="PLT73_09800"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRM20700.1"
                     /translation="MQEPLFIQPVLQEKIWGGTKLRDIYGYDIPSDHTGECWAISAHP
                     DGTGAVENGTYAGTRLDVLYAEHPELFENPTSPVFPLLTKIIDAAEALSVQVHPDDAY
                     GLKHEGELGKTECWYIIDADDDAEIIYGHNAQTKEQFSEMVAAGDWEGLLRHIKVKKG
                     DFFFVPSGTIHAIGGGITILETQQSSNTTYRVYDFDRKDDQGNTRDLHIQQSIDVSMI
                     PHKDPANHFETSTVDGNTVTTFIESDYFTVYKWNILSELAFKKTAPYTLGSVIEGSGS
                     L"
     gene            complement(859..>1416)
                     /locus_tag="PLT73_09805"
     CDS             complement(859..>1416)
                     /locus_tag="PLT73_09805"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM20701.1"
                     /translation="EGLIFRKRGVGTFVIKSALWNSGDSKADEYAGLSKQFPNKTVKS
                     KIILFDILFPPDNIQSLLMLEDNQPVYHLQRLRIIDDKPYILEDTYFVAGLVKDLTED
                     IIHHSIYDHIRDTLGLKIGGAYRKIHADRSNELDWQELACDQHTPILEVEQVVYLTNG
                     TPFEYSTARSRFDTRSYTITDIIKQ"
BASE COUNT          297 a          333 c          393 g          393 t
ORIGIN      
        1 tcaggctgcc gctgccttcg atgacgctcc ccaaggtgta gggagcagtt ttcttgaagg
       61 ccagttcaga aaggatgttc cacttgtaga cagtgaaata gtcgctttcg atgaaagtcg
      121 tcacggtgtt gccgtcgact gtgctggttt cgaagtggtt cgcaggatcc ttgtgcggaa
      181 tcatcgacac atcgatggat tgttggatat gcaggtcgcg tgtgttgcct tgatcgtcct
      241 tgcggtcgaa gtcgtagacg cggtatgtcg tgttgctgct ctgttgcgtc tccagaatcg
      301 tgatgccgcc gccgatggca tggatggtgc cgctcggaac gaagaagaag tcgcctttct
      361 tcactttgat gtggcgcagc agcccctccc agtcgcctgc agcgaccatt tccgagaact
      421 gctcttttgt ttgggcattg tgtccgtaga tgatttcggc gtcgtcatcg gcatcgatga
      481 tgtaccagca ttcggttttc ccgagttcgc cttcatgctt cagtccatag gcatcatccg
      541 gatgcacttg gacggacagg gcttcggcgg cgtcgatgat tttggtcagc aacgggaaga
      601 cgggcgaagt cggattttcg aacagctcag gatgctccgc ataaaggaca tccaggcggg
      661 tgccggcgta ggtgccgttt tccaccgcgc cagtgccgtc gggatgggcg ctgatggccc
      721 agcattcccc ggtatggtcg ctggggatgt cgtacccgta gatatcgcgc agcttggtgc
      781 cgccccagat tttttcttgc aggacaggtt ggatgaacaa tggctcttgc atgtttttcc
      841 tcctaatatt tttatttgtt attgtttaat aatatccgtg atggtgtagg aacgtgtatc
      901 gaagcggctg cgagcagtgg agtactcgaa gggagtccca ttcgtcagat aaacgacttg
      961 ttccacttca agaatcggcg tatgctgatc gcatgccaat tcttgccaat ccagttcatt
     1021 agaacgatca gcgtggatct ttcggtaagc gccaccgatt tttagtccta aggtatcgcg
     1081 gatatggtca tagatggaat ggtggataat atcttcagtc aagtccttga ccaatccggc
     1141 tacaaagtaa gtatcttcca gtatataagg tttgtcgtca ataatcctca ggcgttgcag
     1201 atggtagacc ggctgattgt cttccagcat caacaaggat tggatattgt cgggagggaa
     1261 caggatatca aaaaggatga ttttgctctt taccgtttta ttcggaaact gtttggataa
     1321 gccggcatat tcatccgctt tggaatctcc gctattccag agggcgctct tgatgacaaa
     1381 agttccgaca ccccgtttgc ggaaaatcag gccctc
//
LOCUS       DAOMUJ010000501         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_24996_length_1416_cov_1.589273, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000501 DAOMUJ010000000
VERSION     DAOMUJ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24996_length_1416_cov_1.589273"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLT73_09810"
     CDS             complement(<1..870)
                     /locus_tag="PLT73_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRM20702.1"
                     /translation="MQASNVEATKGQSQIKRKFKVKLLQFKSIRTNLLVGFSIIIVVV
                     FLLGYNSFHASKEINQHTDHIVNVSVPILEQAAEINYLVANRMSFVNSYLLSGDPVYL
                     ELFESYTQKSEAIQSSILEANGNKQVIVELFEGIDEWTRITREEVINVYQAGNQEQAI
                     NTMFEKAEPLSIENIGAMEGALDARSASIHEEGDDVIAHGENVVKVTFIISFFAIVVS
                     VGIALLISSDLMNRIKLLMGRTTELAEGKLGGQPLDIAHFDELGKLGNSINQMQQQLR
                     AIILSISEASNTLE"
     gene            complement(946..>1416)
                     /locus_tag="PLT73_09815"
     CDS             complement(946..>1416)
                     /locus_tag="PLT73_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20703.1"
                     /translation="VFQDCKLLTGKTVFENIAFALEVMGEDTEAISEKVHRSLETVGL
                     ADKADSYSDQLSGGEKQRVAIARAVVHSPKIILADEPTGNLDPRTSLEIFRLFYKINR
                     SGTTILMATHDQGIIENFRFRTLEMKNGYLIKDQDKKEQNTLQYDFKKKEYFIV"
BASE COUNT          400 a          313 c          240 g          463 t
ORIGIN      
        1 ttccagcgta tttgatgcct ctgaaatgct gaggattatg gctctcagtt gctgttgcat
       61 ttgattgatt gaatttccca attttcctaa ttcgtcaaag tgtgcgatgt ctagtggttg
      121 cccacccagt ttgccctctg ctagctctgt cgttcgtccc atcagtaact tgatgcgatt
      181 cattaaatct gaagaaatca ataatgcaat acctacactc acaacaattg caaagaagga
      241 aataatgaaa gtgactttca caacattctc tccatgagcg atcacgtcat caccttcttc
      301 atgaatagaa gcacttctgg catcaagagc gccttccata gcaccgatat tttcaatcga
      361 aagtggctct gctttctcaa acattgtatt tatagcttgt tcctgattgc ctgcttggta
      421 gacgttaata acttcttccc gcgtgattcg agtccattca tcgattcctt cgaataattc
      481 cacaatcact tgtttgttgc cattagcctc caaaatacta ctttgaatag cttcgctctt
      541 ttgtgtgtag ctttcaaaca actccaaata tacaggatca cccgataata gatagctgtt
      601 tacaaatgac atacggtttg caactaaata gttgatttca gcagcctgtt caagaatggg
      661 aacactgaca ttcacaatat gatcagtatg ctgattgatt tctttagatg catgaaaact
      721 gttgtatcct agtaaaaata caaccacaat aataattgag aatcccacaa gtaagttggt
      781 gcggatactt ttaaattgca gtagtttgac cttaaatttt ctttttattt gtgactgtcc
      841 ttttgttgct tctacatttg atgcttgcat tagaaataaa aaaactatgc aaatgaatgc
      901 atagtagttc tcttatcagg tgtgcgttac agcataagac tattattata cgatgaaata
      961 ctctttcttt ttaaaatcat attggagcgt attctgttcc tttttatctt gatcttttat
     1021 tagatagcca ttcttcattt cgagagtccg aaaacgaaaa ttctcaatga ttccttgatc
     1081 atgcgtagcc atcaaaatag tcgtcccact tctgttgatc ttgtagaata aacgaaaaat
     1141 ctccaatgac gttcgcggat ccaagttccc tgtaggctca tccgcaagaa tgattttcgg
     1201 tgaatgtacg acagcacgtg caatcgcaac ccgttgcttt tcaccgccgg acagttggtc
     1261 tgagtaactg tctgctttat ccgccaaccc taccgtttca agcgagcgat gtaccttttc
     1321 cgaaatagcc tcagtatctt ctcccatgac ctccagcgca aaagcgatat tctcaaaaac
     1381 tgttttgcca gtcaacaatt tacaatcctg aaacac
//
LOCUS       DAOMUJ010000502         1413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25045_length_1413_cov_1.632548, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000502 DAOMUJ010000000
VERSION     DAOMUJ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25045_length_1413_cov_1.632548"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLT73_09820"
     CDS             <1..425
                     /locus_tag="PLT73_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20704.1"
                     /translation="EKFSGWLVKEGFKDVGQLHGGIATYGKDETVKGDLWDGKMYVFD
                     ERIGVDINRIEHVVVGKDWFDGTPCERYVNCANPSCNRRILCSEENEHKYMRSCSHEC
                     RVHQPNYYVQRNQLTQSDVEERLAAIGETFENTATTTV"
     assembly_gap    559..613
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            809..>1413
                     /locus_tag="PLT73_09825"
     CDS             809..>1413
                     /locus_tag="PLT73_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRM20705.1"
                     /translation="MKHKLDTETNMKPLLSKGAFGLNVFFGVIRNIFTGIILLMVLLG
                     MFGGGAGLGYFANLISKETAPTYESMAEDIGNVELVSTMYYANSEAISEIRTDLVRTT
                     VGSENISPLIKEALIATEDENFYEHNGIVPKAILRALVTELTGFGSQSGGSTLTQQLV
                     KQQILTNEVTFSRKANEILLALRLENFFTKDEILTAYLNISP"
BASE COUNT          418 a          279 c          318 g          343 t
ORIGIN      
        1 gcgaaaaatt ctcaggctgg cttgtcaagg aaggctttaa ggatgtcggc caactgcatg
       61 gcgggatcgc aacctacggg aaagacgaaa cggttaaagg cgatctttgg gatgggaaaa
      121 tgtatgtctt cgatgagcgc atcggtgtgg atatcaatcg catcgaacat gtcgtggtcg
      181 gaaaggactg gttcgatgga acaccatgcg aacgctacgt gaattgcgcc aatccaagct
      241 gcaaccggag aatactttgt tccgaagaaa acgagcacaa atacatgcgc agctgttcgc
      301 atgaatgccg cgtccatcag ccgaattatt atgtgcaacg taatcaattg acgcagtctg
      361 acgttgagga acgtttggct gccatcggtg aaacatttga gaatactgct actacaacag
      421 tatagcaata acattgtcag caaggataat attccttgct gattttttgt gttcctctca
      481 cttagtgatt gttcacgcta tgtaatagtc agggcagagc ttttttgtta tactgataag
      541 tgaccacctg aaaaggaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnaatccag atggaaataa tgcggaaaaa tggctgaaaa aaatcggcga
      661 atttgttaaa cgccttgggc aatcaataaa tagccgcctc cagcggattt tcacgaaaga
      721 aaatgctgca agaacaaaag ataaagcttt gctgtgggaa caaaaaaccc acaagcgtat
      781 aaaccaaagt aagacttcat tgcgaaaaat gaaacataag ttggacaccg aaacaaacat
      841 gaaaccgctc ctttcaaaag gagctttcgg tctcaatgtt tttttcggtg tcatccggaa
      901 catttttacc ggaatcatac ttctgatggt cttgttgggc atgttcgggg gtggcgctgg
      961 tctgggttat ttcgctaatt tgatttcaaa agaaactgcc cctacctacg aaagcatggc
     1021 tgaagacatc ggaaatgtcg aactcgtttc cactatgtat tatgcgaaca gtgaggccat
     1081 ttcggaaata cgcacagatt tggtcaggac aactgtcgga tcagaaaaca tctctccgct
     1141 catcaaagaa gcactcatcg caacagaaga tgaaaatttt tatgagcata atgggattgt
     1201 acctaaggcc attttaaggg cgttggtgac tgaactgacc ggattcggca gtcaaagcgg
     1261 cggctcaaca ttgacgcagc agctcgtcaa acaacagatt ttgacgaatg aagtgacttt
     1321 ttcccgcaaa gccaatgaga ttttattggc gctacggctt gaaaatttct ttacgaaaga
     1381 tgaaattctg acagcctacc taaatatctc gcc
//
LOCUS       DAOMUJ010000503         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25094_length_1411_cov_1.401917, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000503 DAOMUJ010000000
VERSION     DAOMUJ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25094_length_1411_cov_1.401917"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLT73_09830"
     CDS             <1..439
                     /locus_tag="PLT73_09830"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRM20706.1"
                     /translation="ADKKITKILTVETSGIVPAVFTGLALGVKVVFARKQKSLTMKDN
                     LYSATVYSFTKDVTNEITISKNYLDAEDNVLIIDDFLANGQATNGLMEICNQAGAEIA
                     GVGIVIEKSFQKGRKILEEQGMDVYSLARIKSFENGTVQFIEE"
     gene            614..>1411
                     /locus_tag="PLT73_09835"
     CDS             614..>1411
                     /locus_tag="PLT73_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM20707.1"
                     /translation="MRIETQCLHEGYHPGNGEPSALPIYQSTTYRYDSTEHIGKLFDL
                     TAAGHMYSRISNPTVAAVEEKIAAMEGGVGALCTTSGQAATMISLMNILKEGDHFIST
                     ASIYGGTINLFAVTLKRFGIECTFVDQELPEEEIQKMFKPNTKAVFGETIANPALSVL
                     DIEKWAAIAHKNNVPLIVDNTFPTPYLCRPFEFGADIVVHSTSKYMDGHAVQMGGVIV
                     DSGNFDWKNGNFPEFTEPDESYHGIVYTESFENAAYITKARVQMMRDM"
BASE COUNT          424 a          303 c          344 g          340 t
ORIGIN      
        1 tgccgacaaa aaaatcacca agattctgac ggtggagaca tccggcatcg tgcctgcggt
       61 attcacaggt ttggcgttgg gagtgaaagt tgtttttgca agaaagcaaa agagtctgac
      121 gatgaaagac aatctgtatt cggccactgt ctattccttc acaaaggatg taacaaacga
      181 aatcacaatc tccaaaaact atttggatgc ggaagacaat gtgctgatca tcgacgactt
      241 tttggcgaac ggacaggcaa ccaacggttt gatggagatc tgtaaccaag ccggagcgga
      301 gatcgccggt gtcggtatcg tcatcgaaaa atcattccaa aaaggcagaa aaattctgga
      361 agaacaaggc atggatgtct attcattggc gcgaatcaaa tcctttgaaa acggaacggt
      421 gcagttcata gaagaatagc aagtcaggaa gacagataat ctctgtcttt ttttttcatt
      481 ttcaagcacg ggattttgcc catcatggat ctttcgattt agcttcaata tctctacaaa
      541 gctttttgga attgtggtaa aatgttaaat atatatgaga ttatgagcgc tatcttaatg
      601 aggaggttca ataatgagaa tcgaaacaca atgtttacac gaagggtacc acccagggaa
      661 cggagagcca agcgcgctcc cgatctacca aagcaccact tacagatatg actcgacgga
      721 acatatcgga aaattattcg atttgacggc tgccggacac atgtattcgc gcatctcaaa
      781 tcctactgtt gcagctgtgg aagagaaaat tgcggcgatg gagggtggcg tgggggcttt
      841 gtgcacaact tccggacagg cagccactat gatcagcttg atgaacatcc tgaaggaagg
      901 cgatcatttc atcagcaccg cctccattta tggcggaacc atcaatctgt ttgccgtaac
      961 cttgaagcgt ttcgggatcg aatgcacctt tgtcgatcag gaactgcccg aggaagaaat
     1021 acaaaaaatg ttcaaaccga acacgaaggc cgtattcggc gaaaccatcg caaacccagc
     1081 cttatcagtt ctggatatcg aaaaatgggc agcgattgcg cacaaaaaca acgtgccgtt
     1141 gattgtggac aacacgttcc cgacgccata cctttgcaga ccgtttgagt ttggagcgga
     1201 tatcgtggtc cattccactt cgaaatacat ggatggccat gcagtgcaga tgggcggggt
     1261 cattgtcgac agcggtaatt ttgactggaa aaacggcaat ttccccgaat tcactgaacc
     1321 ggatgagtcc tatcatggca tcgtctatac ggaaagcttt gagaacgctg cttatatcac
     1381 aaaagcacgg gtacaaatga tgagggatat g
//
LOCUS       DAOMUJ010000504         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25262_length_1402_cov_2.277654, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000504 DAOMUJ010000000
VERSION     DAOMUJ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25262_length_1402_cov_2.277654"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..>770
                     /locus_tag="PLT73_09840"
     CDS             269..>770
                     /locus_tag="PLT73_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRM20708.1"
                     /translation="MTQTEEQKALNIDWKNLGFSYIKTDYRFISYWKDGKWDDGEMTT
                     DNKVHISEGSPVLHYGQSCFEGMKAYRTKDGSINLFRPEENAKRMINSCQRLLMPEFP
                     VEKFVEAAKAVVKANEKWVPPYNTGSTLYLRPYLMGVGDNIGVSPATEYIFSIFAMPV
                     GPYFKGG"
     assembly_gap    771..780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <781..1311
                     /locus_tag="PLT73_09845"
     CDS             <781..1311
                     /locus_tag="PLT73_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRM20709.1"
                     /translation="TNFIVSDYDRAAPHGTGAAKVGGNYASSLLPGKEAHDRNFSDCI
                     YLDPATHTKIEEVGSANFFGITKDNRFVTPKSPSILPSITKYSLLYLAEHHLGMQVEE
                     GDVYIDKLDEFSEAGACGTAAVISPIGGIQHGENFHVFYSETEVGPVTRKLYEELTGI
                     QFGDIEAPEGWIVKVD"
BASE COUNT          385 a          342 c          358 g          307 t
ORIGIN      
        1 cataatctaa caaacaaaag ggggagaatg agaatggatg cgacttgcgg aatgtgcaca
       61 ctacagaaca aaaagagagc gttgttctca ttcgatgccc tagtcattct agaagtgatt
      121 tagcccagga ttcttactta ttacaagtag agtcctcttt ccgtttcatt gggctctcat
      181 aaagaatgag cctaatcaca taggctcatt cttttttttc agtatgacag gtaaataata
      241 gagatatgga gtggtttgga tgacagaaat gacacagaca gaagaacaaa aagcgttgaa
      301 catcgattgg aaaaatctag gattcagtta catcaagacg gattaccgct tcatttctta
      361 ttggaaagac ggcaaatggg acgacggcga aatgacgacc gacaacaaag tccacatcag
      421 cgaaggctct ccggtactgc actacggcca atcctgcttc gaggggatga aggcgtaccg
      481 cacaaaagat ggcagcatta acctgttccg ccctgaagaa aacgccaagc ggatgatcaa
      541 cagctgccaa cgcctgttga tgccagagtt tccggttgaa aaatttgtcg aagcggctaa
      601 agcggtcgta aaggcgaatg agaaatgggt tccgccgtac aacacaggca gcacacttta
      661 tctgcgtcca tacctgatgg gagtcggcga caacatcggc gtcagcccgg cgaccgaata
      721 catcttctcc atcttcgcga tgccggtagg cccttacttc aaaggcggct nnnnnnnnnn
      781 acgaacttca tcgtgtccga ctacgaccgc gccgcaccgc atggtacggg tgcagcgaaa
      841 gtcggcggta actacgcaag cagcttgctg cccggtaaag aagcgcatga tcgcaacttc
      901 agcgactgca tctatcttga tccggcaacg cacacgaaaa tcgaagaagt tggctccgca
      961 aacttcttcg gcatcacgaa ggacaaccgc tttgtgacgc cgaaatcacc ctcgatcctg
     1021 ccgagcatca ccaagtactc tttgttgtac ttggccgagc accacttggg catgcaagtc
     1081 gaagaaggtg atgtctacat cgacaagttg gatgaattca gcgaagccgg cgcgtgcggt
     1141 accgcggctg tcatctcacc aatcggcggc atccagcatg gggaaaattt ccatgtcttc
     1201 tattcggaaa cggaagtcgg cccggtgacg cgcaaattgt atgaagaact gaccggcatc
     1261 caattcggcg acatcgaagc gccggaaggt tggattgtga aagtggactg atccgattga
     1321 acgaaatgca gctctcccaa agcttttatg ctggcggagg gctgtttttt ttggttctgg
     1381 ctgccggaac aggtcgccag ct
//
LOCUS       DAOMUJ010000505         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25265_length_1402_cov_1.711952, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000505 DAOMUJ010000000
VERSION     DAOMUJ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25265_length_1402_cov_1.711952"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..>550)
                     /locus_tag="PLT73_09850"
     CDS             complement(251..>550)
                     /locus_tag="PLT73_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20710.1"
                     /translation="GIVTLGLCFGGVMGVFPGLVMDNFGPANQGVNYGIVFIGFSSAA
                     FVAPKVTASLAAANNGDFTKAFYVAIAVVLIGLGLSLVYAKRKADAVEAAVETIQ"
     assembly_gap    551..626
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<627..>1402)
                     /locus_tag="PLT73_09855"
     CDS             complement(<627..>1402)
                     /locus_tag="PLT73_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20711.1"
                     /translation="MMAFAINSAVGPIPMIFGGYFTDRGWAKWIARIGVLLFGISFAF
                     TGQATSIFQLYLIYGIFGGFAQNFAYSACLSNTIRFFPDKKGLASGLITGGMGGAAII
                     AAPIANALIERYGVSQAFWRMGLAYALVGFFFSLFIRSAPKQIVPQTAATSEKAPTKA
                     ITANKNWKEMLATPAFYLLILMFAMGAFSGLMIASNASLIGQSMFGLSASAAAAYVSL
                     YSLSNTSGRVFWGAVSDRIGQIRAVIIIYSAIIFAFVLLV"
BASE COUNT          354 a          342 c          337 g          293 t
ORIGIN      
        1 aaaccgttat tatttcgttt ttttgagcgc attattattt ctcctctttt ctgaaagcca
       61 actataaaat aattgtcggt gtttactata ttttagatta ctttagccgg ttagtaaagt
      121 cgttttgaaa tctttagaca gccgttccaa aaggctgcat ctccattatt tccaatccag
      181 ataaaaacac ccaaaaatca gcagtaacct gatttccggg tgtttgacta tccgtgcatt
      241 catgcgcttg tcattggatt gtttctacgg ctgcttccac agcatccgct tttcttttcg
      301 catagacgag gctcaagccc agtccgatga ggactacagc aatcgcgaca tagaaggctt
      361 tcgtgaaatc gccattgttt gcggcagcca atgaggcagt cactttcggc gcaacgaagg
      421 cggcactcga aaaaccgatg aagacgatgc cgtaattgac gccctgattc gccggaccaa
      481 agttatccat gaccaatccc gggaagacgc ccatgacccc gccaaaacag agacccaaag
      541 tgacgatccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnngacg agcaggacga aagcaaaaat gatggcactg
      661 tagatgataa tgaccgcgcg gatctgaccg atgcgatcgg aaaccgcgcc ccagaatacc
      721 cggccggacg tgttgctcaa ggaatagaga ctgacatagg cggctgcggc tgatgccgac
      781 aaaccgaaca tggactgccc gatcaaagag gcgttcgaag cgatcatcag gccggaaaag
      841 gcgcccatcg cgaacatcag gatcaacagg tagaaggctg gggtcgccaa catttccttc
      901 cagtttttgt ttgcggttat ggctttggtt ggagcctttt cggaggtcgc agcggtttga
      961 ggaacgattt gtttcggtgc cgagcggatg aacagactga aaaagaaacc taccagcgca
     1021 tatgccaagc ccatgcgcca gaaagcttgg ctcacgccat agcgttcgat cagcgcattc
     1081 gcgatcggag ccgcgatgat cgcagctccg cccatgcctc ccgtgatcag accggaagcc
     1141 aagccttttt tatccgggaa aaagcggatt gtgttgctga ggcaagcgga ataggcaaag
     1201 ttctgcgcaa agccgccgaa gatgccgtag atcaggtaca actggaagat gctcgtcgct
     1261 tgtccggtga aagcgaagct gataccgaaa agcaaaacgc cgatgcgggc gatccatttt
     1321 gcccagccgc ggtccgtaaa atagccgccg aaaatcatcg gaatcggccc caccgcggaa
     1381 ttgatcgcga aagccatcat ga
//
LOCUS       DAOMUJ010000506         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25427_length_1395_cov_1.718657, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000506 DAOMUJ010000000
VERSION     DAOMUJ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25427_length_1395_cov_1.718657"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PLT73_09860"
     CDS             complement(<1..1011)
                     /locus_tag="PLT73_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20712.1"
                     /translation="MSGSAWSKTIPLKEQLRVIKRIFRFAHPFRYQFFIALLFSIALS
                     LVNVVAPRIIQIYMDDYLAVGDVTTNISLFFAMAYLGSILLKMLVTYLQRYIFSMASE
                     QTVENIRNAVFRKINQLGMRYYDTTPAGSIVSRVTNDTETIKEFWNVFLALAEGIFSI
                     ITVFIAMWTLDKQISLLFIVFVPIMAGLIWYYQKYSTRVYRTMRERLSQLNTKLNESI
                     SGMAIIQQFRQEKRLRAEFDAINDDYSKGRVAMVQMNALMLMPVVNLLQAIALAIVLW
                     LFGYQTLNGVVELGVIYAFTTYIQNFFRPMGMMMDNLSALQDGIVSSSRVLSVLDNAE
                     LAP"
     gene            complement(1008..>1395)
                     /locus_tag="PLT73_09865"
     CDS             complement(1008..>1395)
                     /locus_tag="PLT73_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20713.1"
                     /translation="LGFPAGYDTMVGERGVSLSGGQKQRIAIARALVTEPELLILDDS
                     LSAVDAKTEEAILTGLKQQRVDQTTIIAAHRISSVMHANEIIVLDEGRIVERGTHHEL
                     MELNGWYRRMYEKQQLETKLEGKDEA"
BASE COUNT          388 a          339 c          296 g          372 t
ORIGIN      
        1 tggcgccaat tcggcgttat ccaatacact caggaccctg gaactggaga cgataccgtc
       61 ctgcagggcg ctgaggttgt ccatcatcat tcccatagga cggaagaaat tttggatata
      121 ggtagtgaat gcatagatga cccccaactc cacaacgccg ttcaaagtct ggtagccaaa
      181 taaccaaagg acaatcgcca aagcaattgc ctgcaatagg ttgacgaccg gcatcaacat
      241 caaagcattc atctggacca tcgctacgcg acctttactg taatcatcat tgattgcatc
      301 aaattctgcg cgcagacgtt tttcttggcg gaactgctga atgattgcca taccggaaat
      361 ggattcattt aatttcgtat tcagctggct cagtctttca cgcatcgtcc ggtaaacacg
      421 tgtgctgtat ttttgataat accaaatcag acctgccatt atcgggacaa aaacgatgaa
      481 gagcaaggaa atctgcttgt ccaatgtcca catggcgatg aacacggtaa tgatgctgaa
      541 aatcccttct gccaaagcca aaaacacatt ccagaattct ttgatggttt ctgtgtcatt
      601 ggtgacgcga gaaacgatcg aaccagctgg agttgtatca taataacgca tccccaattg
      661 attgatttta cggaaaacag catttcggat attctcgaca gtttgttcgg atgccatgct
      721 gaatatataa cgctgcagat aagtgactaa catcttcagc agtatggatc ccagataggc
      781 catcgcaaaa aacagactga tattggtcgt gacatcgccc acagccagat aatcatccat
      841 ataaatttgg atgattcgcg gggccacaac attcaccaac gataacgcga tactgaaaag
      901 caaggcgatg aaaaattgat aacgaaaagg atgggcaaat cggaaaattc ttttgattac
      961 ccgaagctgt tccttcaatg ggattgtctt agaccatgct gatccactca tgcttcatcc
     1021 ttcccttcca attttgtttc gagttgttgt ttctcataca ttcttcggta ccacccgttc
     1081 aattccatta actcatgatg agtcccgcgt tcgacgatgc gtccttcatc aagaacgatg
     1141 atttcattcg catgcatcac actgctgatc cgatgggctg cgatgattgt tgtctgatcg
     1201 actcgttgtt gcttgagtcc cgtcaagatt gcttcttcgg ttttcgcgtc cacagcagat
     1261 aaagaatcat ccaaaataag cagttctggt tcggtgacta aggctcgcgc aatcgctatc
     1321 cgttgttttt gcccacctga cagagaaaca cctctctcgc cgaccatcgt atcgtatcca
     1381 gcggggaagc caagg
//
LOCUS       DAOMUJ010000507         1379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25778_length_1379_cov_2.330060, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000507 DAOMUJ010000000
VERSION     DAOMUJ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25778_length_1379_cov_2.330060"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLT73_09870"
     CDS             <1..458
                     /locus_tag="PLT73_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM20714.1"
                     /translation="GNILWITDPEVAIWSIIIVGIWTNVGYNMILLLAGLKEIPKEYY
                     EAAVVDGAGPLKQFFSITLPLLSPQLFFVSVTSVIGALQMFDIIFMMFDSTNLALKST
                     QSLVYMFFNESFVLNDKGYGSAIAISLVLIIMAITGIQLLAQKKWVTYD"
     gene            471..1295
                     /locus_tag="PLT73_09875"
     CDS             471..1295
                     /locus_tag="PLT73_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM20715.1"
                     /translation="MQTKMNKTTALIHIVLIIGSLLMIVPFLWMVLTSGKTISESTQI
                     PPQIFPEVFQIENYKAVWNSLPFVSFYINTGLMIVFRVLTSTLFSAMAAFAVAKLDFP
                     GKNLFFAIVLTQMMIPSQIYLTPQYLLVQNLGLLNSIPALVIPGIVSAFGTFLLRQFF
                     IKLPDELMEAATIDGATTWQVFYRIYMPLARSGLVALGIFTSLFAFKDLMWPLVVNMS
                     QDKMTLSAGLASLQGQFTTNYPQLMAGSVIAIIPMVILYILFQKQFIEGIATTGGK"
BASE COUNT          328 a          338 c          333 g          380 t
ORIGIN      
        1 ccggaaatat cttatggatc accgatccgg aagtggcgat ctggtcgatc atcattgtcg
       61 gaatttggac gaacgtcggc tacaacatga tcctgctgtt ggcggggctg aaagaaatac
      121 caaaagaata ttatgaggcg gctgtcgtcg acggcgccgg accgttgaaa caatttttct
      181 cgatcacgct accgttgctt tcaccgcaat tgttcttcgt ttccgtgacg agcgtcatcg
      241 gtgcgctgca gatgttcgac atcattttca tgatgttcga cagcacgaat ttggcgctga
      301 agagcacaca gtcactggtg tacatgtttt tcaatgaatc atttgtgctc aatgacaaag
      361 gctacggttc ggctattgcc atctcgttgg tgctgatcat catggcgatc accggcatcc
      421 agctattggc acagaaaaaa tgggtcacct acgactagga gtgaataaac atgcagacaa
      481 aaatgaataa aacgacggca ctgatccaca tcgtgctgat catcggatct ttactgatga
      541 tcgtgccgtt cttatggatg gtcctgacct ccggaaaaac catcagtgaa tcgacacaga
      601 tccctccaca gattttcccg gaagtattcc agatcgagaa ctacaaagcg gtatggaatt
      661 ccttgccgtt cgtcagtttt tacatcaaca ccggtttgat gattgtcttc agggtgttga
      721 cttcgacgct cttcagcgcg atggcagcct ttgcggttgc taagctggac tttccaggca
      781 aaaatctgtt ttttgccatt gtattgacac agatgatgat tccttcacaa atctatctga
      841 caccgcaata tttgttggtc cagaatctgg gtcttttgaa ctcaatcccg gctttggtta
      901 tcccgggtat cgtttctgct ttcggaacgt tcctgttgcg ccaattcttc ataaaattgc
      961 cggatgaact gatggaagcc gccacgattg atggtgcgac gacctggcaa gtgttctacc
     1021 gcatctatat gccgttggcg cgttccggct tggtggcttt gggcatcttc acgtctctgt
     1081 tcgctttcaa ggatttgatg tggccactcg tcgtcaacat gtcccaggat aaaatgacgc
     1141 tgtcggccgg tttggccagc ctgcaagggc aattcacgac gaactatccg cagctaatgg
     1201 ccggttcggt catcgcgatc atcccgatgg ttatcctata tatcctgttc caaaaacaat
     1261 tcatcgaagg catcgccaca accggcggga agtaagaaaa gctgaacggg ttcgcttagc
     1321 cctgaaagaa ttttaggaat ctgtcctcgc aacgttccct acggtcacct tgtactggg
//
LOCUS       DAOMUJ010000508         1376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25869_length_1376_cov_1.710825, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000508 DAOMUJ010000000
VERSION     DAOMUJ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25869_length_1376_cov_1.710825"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLT73_09880"
     CDS             <1..371
                     /locus_tag="PLT73_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM20716.1"
                     /translation="QSSTTDYNGVYKGLYLDFEAKETKSKTAIPLSNFHEHQIVHMKR
                     CIQQQGITFVLIRFSVFNRVFLLNSSYLIDFWENTLNNGRKSIPLVFIEENGIEVAYG
                     FKPRLDYLDAVDKMLERTIK"
     gene            389..>1376
                     /locus_tag="PLT73_09885"
     CDS             389..>1376
                     /locus_tag="PLT73_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM20717.1"
                     /translation="MPNEGNHTRSRANNKQKNGKTKSHRKSTVKATKKAKKNGMPLWK
                     KIIFGIFALGIIGLASGAGLFFYYVSSAPELTEEKLAGPAASVILGPDGEEIKTIGGS
                     EQARTVVTAEEIPQVLKDAIVSIEDQRFYDHKGVDPIRILGALFANLQQGGISQGGST
                     ITQQLIKLSFFSTSSEDQTLERKAQEAWMSMQLEREYTKDEILAFYINKVYMSNNVYG
                     MGTAAEYFFGKSITDVTLAEAATLAGMPQAPSLYDPINNSEDTQARRDLVLDMMVENE
                     KITAEESAEAKTVQIQGSILDHSGDVNTSLVIDPYIQLVIDEVKEKTGLDVYE"
BASE COUNT          419 a          331 c          327 g          299 t
ORIGIN      
        1 gtcagtcctc cactaccgac tataacggtg tttacaaagg gctatatctg gactttgagg
       61 ctaaagaaac aaaaagcaag acagccatac ccttgagcaa cttccacgag catcaaatcg
      121 tccatatgaa aaggtgtatc caacagcaag gaatcacgtt cgtactgatc cggttttctg
      181 tcttcaaccg tgtcttctta ctgaacagtt cctatttgat tgacttttgg gagaatacat
      241 tgaataatgg ccgtaaatcg atccctttgg tatttattga ggaaaacggg atagaagtgg
      301 catacgggtt caaaccccga ttggattatc tggatgcagt ggacaaaatg cttgaacgca
      361 ctatcaaata acttaagagg tgaaccacat gccgaatgaa ggcaatcata caagatcacg
      421 cgcaaacaac aaacagaaaa acggaaaaac aaaaagccat cgcaaaagta ctgtgaaagc
      481 aacaaagaaa gcgaagaaaa acgggatgcc gctctggaaa aaaattatct tcggtatttt
      541 tgccctaggt atcatcggac tcgcttcggg cgctggcctt ttcttctact acgtctcttc
      601 cgcccccgaa ctgaccgagg agaaattggc cggaccagca gcatcagtta tcctgggtcc
      661 cgacggcgag gaaatcaaga cgatcggcgg cagcgaacag gcccgcacgg tcgtgacagc
      721 agaggagatt ccgcaagttt tgaaggatgc catcgtatcg atagaagatc aacgcttcta
      781 cgatcataag ggtgtcgatc cgatccgaat cctcggtgcg ctgttcgcga atcttcagca
      841 gggcgggatc tcgcaaggcg gaagcaccat cacccagcaa ttgattaaat tatccttttt
      901 ttcaaccagt tccgaagacc agacgctgga gcgaaaggct caggaagctt ggatgtccat
      961 gcagctggaa cgggaatata caaaagacga aattttggcg ttctacataa acaaagtcta
     1021 tatgtcaaac aacgtgtatg gcatgggcac ggccgctgaa tactttttcg gcaaatccat
     1081 caccgatgtg acgcttgctg aagcagccac tttggcgggc atgccgcaag ctccttcact
     1141 gtatgaccca atcaacaatt cagaggatac gcaagccaga cgcgaccttg ttctggacat
     1201 gatggtcgag aacgaaaaaa tcaccgccga agaaagcgcc gaggcgaaga cagtccaaat
     1261 tcagggcagc atactcgatc actccggtga cgtcaataca tcactggtga tcgatccata
     1321 tatccagctc gtcatcgatg aagtcaagga gaaaaccggc ctggatgtct acgaag
//
LOCUS       DAOMUJ010000509         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_25903_length_1374_cov_2.400303, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000509 DAOMUJ010000000
VERSION     DAOMUJ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25903_length_1374_cov_2.400303"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1374)
                     /locus_tag="PLT73_09890"
     CDS             complement(<1..>1374)
                     /locus_tag="PLT73_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRM20718.1"
                     /translation="TEETSDISSTQESSEEEIVNPNSSVEESKPVIDKEELVPEKPTT
                     APVIRPNQSTTNTVTPTISEQIFPTLSQPFDLPLSTQSFVASDLNGFVLPLLSTYADQ
                     REAAIVVTALSYLNQPYKKGGKGPEHFDNLNYSRYVYQQIFSLDIPEEQTKFLQIGTK
                     VTLDETRPGDLVVWEKENKVGIYLGQNKIIMADDSLLNDSFQRSNESEEIPGVRIFTL
                     RDEQELGDEDEDQDLLTRYDFLRVPDYGLAKKEEWQLSTYGLQQVRDYPVSFAFQEND
                     VTNAFIDSIGELAREMALKYDVFASVMIAQAILESGAGTSGLSRAPYYNLFGIKGSNS
                     GASITLPTAEDNGQGQLYTINAAFRVYPSYRESLEDYVELIRTGISGNQTFYQKSWRS
                     TAKNYLSATQNLMGKYATDTFYANKLNSLIATYQLTRFDEPKVSVSHAMMTLSEIPLE
                     YRQDIRFP"
BASE COUNT          379 a          294 c          270 g          431 t
ORIGIN      
        1 cggaaagcgt atgtcttgtc gatattccag tgggatttca gagagtgtca tcatcgcatg
       61 actgactgaa actttcggtt cgtcaaaacg tgtgagttga taggttgcaa taagtgaatt
      121 taatttattc gcataaaaag tatccgttgc atatttgccc atcagatttt gtgtagcact
      181 taaatagttt tttgccgtgg aacgccaaga tttttgataa aaagtttgat ttccactaat
      241 tccggttcga attaattcaa cataatcttc aagcgactct cgataacttg ggtacactcg
      301 aaaagcggca ttgatcgtgt aaagctgccc ttggccatta tcttctgcag tcggcaacgt
      361 aatagaggct ccgctattgc ttcctttaat accaaataaa ttataataag gtgcacgcga
      421 taacccactc gtccccgcac cactttctaa aattgcttgg gcaatcatga ccgaagcaaa
      481 gacgtcgtat ttcaaggcca tctctcttgc caattcacca atcgagtcaa taaacgcgtt
      541 ggtcacatca ttttcttgaa atgcaaacga aacaggatag tctctgactt gctgcaagcc
      601 gtatgtggat aattgccatt cctctttttt ggcaagacca taatcgggta ctcgtaaaaa
      661 atcataacgc gttagtagat cttggtcttc atcctcatca cctaactcct gctcatcacg
      721 taacgtaaag atgcgaacac ctggaatttc ctcagactca ttcgatctct ggaatgagtc
      781 gttcagtaac gaatcatctg ccatgatgat tttattttgg ccaagataaa ttccgacttt
      841 attttctttt tcccagacga ctaaatcacc gggtcttgtc tcatcaaggg taacttttgt
      901 cccaatttga aggaattttg tctgctcttc agggatatcc agcgaaaaaa tttgttgata
      961 aacataacga gaatagttca aattatcaaa atgttccggc ccttttccac cctttttgta
     1021 aggttggttc aaataactta aagccgtcac aacaatcgcc gcttcccgtt gatctgcgta
     1081 agtgcttagt aagggtaaaa caaaaccatt taaatccgat gccacaaacg attgggtact
     1141 cagcggtaga tcgaacggtt ggctgagtgt tggaaagatt tgttcagaaa tagttggcgt
     1201 tacggtattt gtagttgact gattcggacg tattacagga gcagtcgttg gtttttcagg
     1261 aaccaattct tccttatcga tcacaggctt cgattcttcc acagaactat tcggattgac
     1321 aatctcttct tcgctgcttt cttgtgtgga agaaatgtct gaagtctctt ctgt
//
LOCUS       DAOMUJ010000510         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26044_length_1370_cov_1.428137, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000510 DAOMUJ010000000
VERSION     DAOMUJ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26044_length_1370_cov_1.428137"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PLT73_09895"
     CDS             <1..147
                     /locus_tag="PLT73_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRM20719.1"
                     /translation="EGILVGISSGAAVAGAIALAGQLGAGKSVVTIAPDNGERYLSTP
                     VFQN"
     gene            383..>1370
                     /locus_tag="PLT73_09900"
     CDS             383..>1370
                     /locus_tag="PLT73_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HRM20720.1"
                     /translation="MNPVVIIPALNPDEKLILLVEELQKKDLLTIVVDDGSGAEYAAI
                     FELLKSKYACDVCTHPSNCGKGRALKTGIRFLLDKYPDSTGFVTADADGQHAVSNIGE
                     IAGALGGNPQALVLGVRNFKDQGIPFRSRFGNKMTSSIFRAATGTRCQDTQTGLRGVP
                     MQYADLCLGLTGERYEYEMNMLMTFAENKIPFRQVPIETIYIENNRASHFDPIKDSVR
                     IYWNILKYSLSSALSALVDLSLFGLFSTYLFPSMTAKIGLATLLARCISGAVNFMLNK
                     HWVFQNERSLGSQGIKYGALFITQLFLSGLLVELLSDLPIPLLLVKAFIDASL"
BASE COUNT          339 a          320 c          362 g          349 t
ORIGIN      
        1 gaaggcattc tggtgggcat ctccagcggt gcggcagtgg ctggagccat cgccttggcc
       61 ggtcaacttg gcgcaggcaa gtcggttgtg acgatcgctc cggataacgg cgaaagatac
      121 ctttccaccc cagtgtttca gaattaagca atataccgaa taatgatagt gccccaacca
      181 cagcaggagc ggttcctgtg gttggggcac tttttttgct ccaaacttgg tgagggcttt
      241 tatttgtgta tgtttcgcta ctttttttcc gcaactattc gtatggattt cgaggaattc
      301 agtgggtgcg ttcttttaaa atggaatcag caccagaaaa ggagcaacgg ttggccggac
      361 tgaaaaatta ggaggcgtca atatgaatcc agtcgtaatc attccagctc ttaaccctga
      421 cgaaaagttg attttattgg ttgaagagct acagaaaaag gatctgctca ctatcgtggt
      481 ggacgatggc agcggtgcgg aatatgcagc aattttcgaa cttttgaaaa gcaaatacgc
      541 atgcgatgtc tgcacgcatc ccagcaattg cggaaaagga cgggccttga agacgggcat
      601 cagatttctt ttggacaagt atccggacag cactggtttt gtgacggcgg atgcagatgg
      661 gcaacacgcg gtctcaaaca tcggggaaat cgccggcgcg ctgggcggaa atccgcaggc
      721 actggtgttg ggcgtacgga atttcaagga tcagggcatt cccttccgca gccgcttcgg
      781 caacaaaatg acttcttcga ttttccgcgc cgcaacaggc acccgttgcc aagataccca
      841 gaccggactg cggggcgttc cgatgcagta tgctgatctg tgcctcggac tgacagggga
      901 aagatatgaa tatgagatga atatgctcat gacatttgcg gaaaacaaaa tcccttttag
      961 gcaagtgccg atagagacca tctacatcga gaataaccgc gcctcgcatt tcgacccgat
     1021 caaggactcg gtccgcatct attggaatat cctgaaatac agtctgtctt cagcgctgtc
     1081 agcactggtg gacctctctt tgttcggatt gttcagcacc tacctgttcc catccatgac
     1141 agcaaaaata ggccttgcga ccctgttggc cagatgcatc tccggagctg tgaattttat
     1201 gctgaataag cattgggtat tccaaaatga acggtcattg ggaagccaag gaatcaaata
     1261 tggcgctctc ttcatcacac agttgttttt gagcggactg ctggtggagc ttttgagcga
     1321 cttgccgatt ccgttgttgc ttgtaaaagc attcattgat gcatcgctct
//
LOCUS       DAOMUJ010000511         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26274_length_1360_cov_1.642912, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000511 DAOMUJ010000000
VERSION     DAOMUJ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26274_length_1360_cov_1.642912"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLT73_09905"
     CDS             <1..322
                     /locus_tag="PLT73_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20721.1"
                     /translation="ATDQVGLGAGELDRSLDRLSDVQRTLIGFADVPRSMAELMAHAG
                     FSHRPHLVTTHIEPLLAGGVLRLTLPEKPRSPKQRYVLTEAGVKLKLLHDQAACPTEE
                     DNGH"
     gene            312..>1360
                     /locus_tag="PLT73_09910"
     CDS             312..>1360
                     /locus_tag="PLT73_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM20722.1"
                     /translation="MDIETSGAIRLFFPNPSLTLVYFEALANALDAGATEISIDIDVQ
                     AFDKPDTLKITVSDNGDGFTDENFERFRKLLRPRDKFHKGIGRLVFLNYFSRVDVTST
                     RSKWRRTFIFKDGFDGNAPIENLNDEQPAKTTLAFNGFAKDRVKSYDDLKPDVLKPLL
                     IEQFLPTLDARKRDGTAFKISINLKTKESNAQKEFFPHETVITADDLPSMTKITIQDD
                     SLDAFSTIDMHYHVEPVSGKGSLLVAFSIDGRTIPVNLIPASSFPPGYMGMFLFESEM
                     FHSNADSSRQKLILPENLPIEKLYRVLRREVGKVLADMIPKITEKNEKVKEKFEEQFP
                     HLLGYFENDTVGLIDR"
BASE COUNT          350 a          349 c          343 g          318 t
ORIGIN      
        1 agctaccgat caagttggtc ttggtgcggg tgagcttgat cggtcgcttg atcggttgtc
       61 cgatgtgcag cggacgctca ttggatttgc agatgtgccg cgctcgatgg cagaactgat
      121 ggcccacgcc ggtttcagcc atcgcccgca cctcgtcaca acccacattg agcctttgct
      181 ggctggcggc gtgctgcgcc tcacgctacc tgaaaagcca cggtccccca aacaacgcta
      241 tgtcctcacc gaggcaggcg tgaagcttaa gctattgcac gatcaggcgg catgcccaac
      301 ggaggaagac aatggacatt gaaacctctg gggccatcag gctgtttttc cccaacccgt
      361 cgttgacatt ggtttatttt gaagcgcttg ccaatgcgtt ggatgctggt gcgaccgaaa
      421 tatccataga tattgatgtg caggcattcg acaagccgga cacactgaaa atcacggttt
      481 cggacaacgg tgatggattc acagacgaaa actttgagcg tttcagaaaa cttctgaggc
      541 ccagggacaa gttccacaaa ggcatcggtc gcttggtatt cctcaactac ttcagccggg
      601 tggatgttac tagcacccgc agcaaatggc gcaggacctt tatcttcaaa gatggcttcg
      661 atggcaatgc accaatcgaa aatctgaatg atgagcagcc agccaaaaca acattggcat
      721 tcaatggctt tgcaaaagat cgcgttaagt cctatgacga tctgaaacct gatgtgctca
      781 agccactctt gatcgagcaa ttcctgccga cgctggatgc gcgcaagcgt gacggcacag
      841 cattcaaaat ttccatcaac ctgaagacca aagaaagcaa cgcacaaaaa gagtttttcc
      901 cgcacgaaac ggtcattaca gccgatgact tgccttcaat gaccaagatc accatccagg
      961 atgattctct ggatgcgttt tcgaccattg acatgcacta ccacgtcgaa ccggtgtcgg
     1021 gaaaaggaag cctgctcgtt gccttcagca ttgatggtcg gacgattcca gtcaacttga
     1081 taccagcttc gtcttttcct cctggctaca tgggtatgtt tctgtttgag tcggagatgt
     1141 ttcactccaa tgccgacagt tcgcgtcaaa aactgatact tccggaaaat ttgccaatag
     1201 agaaacttta tcgcgtcttg cgccgggagg ttggcaaggt gttggcagac atgattccga
     1261 agatcacgga gaagaacgaa aaggtaaagg aaaaattcga ggagcaattc ccgcatcttc
     1321 tgggctactt cgaaaacgac accgttggct tgattgatcg
//
LOCUS       DAOMUJ010000512         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26477_length_1352_cov_1.987664, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000512 DAOMUJ010000000
VERSION     DAOMUJ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26477_length_1352_cov_1.987664"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLT73_09915"
     CDS             complement(<1..483)
                     /locus_tag="PLT73_09915"
                     /inference="COORDINATES: protein motif:HMM:NF023523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HRM20723.1"
                     /translation="MSRNQNQNQDTYANLTMTGMGYINRPRWVQPDEGDAYLSMSINA
                     WHGKNGKKSTLIETSPRGEQANRVIDELLTAYPELMEGSRESHGLTVTIGFMVGDCEP
                     KSFVSRDKKTTHYIKSRLFLVNWIKVNGQYFYRKNDGKAEAHEEIPAMDAGENHQQPE
                     P"
     assembly_gap    618..672
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(725..>1352)
                     /locus_tag="PLT73_09920"
     CDS             complement(725..>1352)
                     /locus_tag="PLT73_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM20724.1"
                     /translation="REIIDGAKQKGFYIANIYEEKASGVNASRPELNRLINDLQDGDW
                     VIAERLDRLTRLPPREAEMLIQRITEKGAKLYVPDVFSFGEIDDLMGKGDSLPKYLAE
                     PLMEALQKMFLRMALSMAHEDWKTRRERTQQGLERAKSEGRMKGRPADKQLHKDIIQL
                     ALQGVSITRTAGILKCSESTVSKVRRKHKEKHPSAAKDQLDIFKAKNT"
BASE COUNT          299 a          317 c          294 g          387 t
ORIGIN      
        1 cggttcaggt tgctgatggt tttcaccggc atccatcgca ggaatttctt cgtgcgcttc
       61 agctttgcca tcatttttgc gataaaaata ttgaccgtta actttaatcc agttcaccaa
      121 gaacaaacgg gatttgatgt aatgggtcgt ttttttatct cttgagacga agctcttggg
      181 ttcacaatca cctaccataa aaccgatggt gacagtaagg ccgtggcttt cacgactgcc
      241 ttccatcagt tccggataag ccgtaagcaa ttcatcgatt acacgattgg cttgctctcc
      301 ccgggggctg gtttcgatca aagtgctttt tttgccgttt tttccatgcc aagcattgat
      361 tgacatggat aaataggcat caccttcatc gggttgaacc cagcggggac gattgatata
      421 gcccatgccg gtcatggtca gattggcgta ggtgtcttga ttttgatttt gattgcgaga
      481 catgatgtat tactcctgta taaaaaacag gggatacatc atccctagtg gggatttgta
      541 tccccaaagg gtttgataag aatagtagtc ggcatggccg acgggtgaaa acaagttact
      601 gctccaacaa aggaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nngaagcaac aaacactcgg tacactttta tgttgaacca atataattag
      721 catgtcaagt atttttcgcc ttgaaaatat ccagttggtc tttggcagcg gacggatgct
      781 tttctttatg cttgcgccgc actttgctga cggtggattc gctgcatttg agtattccgg
      841 ccgtgcgggt aatgctgaca ccctgtaagg ccaattggat aatatcttta tgcagctgct
      901 tgtctgctgg acggcctttc atgcgccctt ccgatttggc gcgttcaagc ccctgctgcg
      961 tacgctcgcg ccgggttttc caatcctcgt gagccatgct cagcgccatg cgtagaaaca
     1021 ttttttgcaa tgcctccatc aaaggttcgg ccaagtattt gggcaatgaa tcgccctttc
     1081 ccatcaagtc atcgatttcc ccgaatgaaa acacatcggg tacatacagt tttgctcctt
     1141 tttcggtaat acgctgtatc aacatctccg cctctcttgg tggcagacgc gtaagccggt
     1201 ccaagcgttc ggcgataacc caatcgccat cttgcagatc attgatcaac ctattcagct
     1261 ccggtcgact ggcgttgacg ccgctggctt tttcttcata gatattggct atataaaatc
     1321 ccttttgctt agccccatca attatttcac gc
//
LOCUS       DAOMUJ010000513         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26688_length_1343_cov_1.680124, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000513 DAOMUJ010000000
VERSION     DAOMUJ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26688_length_1343_cov_1.680124"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLT73_09925"
     CDS             complement(<1..783)
                     /locus_tag="PLT73_09925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM20725.1"
                     /translation="MQANKNAFPKDFLWGSASAAYQVEGAWNEDGKGPSIWDEFVKVP
                     GTTFKNTNGDVAVDHYHRVEEDIRLMAEMGLKTYRFSIAWARIFPTSMDEVNQKGLDF
                     YHKLIDLCLENNVEPMVTIYHWDLPQFLQEKYRGWESREVITDFKKYAVTLFEAYGSK
                     VKYWITINEQNIFTRMGWLTAQHPPKMSGEEKLYYQVNHNVFLAHAEAVLAFQDLVPD
                     GMIGASFALHPSYAIDCKPENVVAKMDYDDLKNYWWMDVYAHG"
     gene            999..>1343
                     /locus_tag="PLT73_09930"
     CDS             999..>1343
                     /locus_tag="PLT73_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HRM20726.1"
                     /translation="MPIHIHQQGKLFHLTNQKISYIMQVLEDGSLGNLYFGKKVADRT
                     DFSHLIESAYRPNTAYASQKDYSFSREHTRQEFPVYGTTDFRHPAVSVLHENGSRVSA
                     FQVKGHAVHSGKK"
BASE COUNT          327 a          354 c          313 g          349 t
ORIGIN      
        1 cccatgcgcg taaacatcca tccaccaata gtttttcaga tcgtcgtaat ccatctttgc
       61 tacaacattt tccggcttgc agtcgatggc gtagctcgga tgcagcgcga agctcgcacc
      121 aatcatgccg tccggaacca gatcctggaa ggcgagcacg gcctcggcat gcgccaagaa
      181 cacgttatgg ttgacctggt aatagagttt ttcttcgccg ctcattttcg gcggatgctg
      241 cgccgtcagc cagcccatcc gtgtgaagat gttctgctcg ttgatcgtga tccaatattt
      301 caccttgctg ccgtacgcct cgaacaaagt cacagcgtat ttcttgaagt cggtaatgac
      361 ttcccgactt tcccaaccgc ggtatttttc ctgcaggaac tgcggcagat cccagtgata
      421 gattgtgacc atcggctcga cgttgttttc gaggcacaga tcgatcagct tgtgatagaa
      481 atccaagccc ttttggttca cctcatccat ggaagtcggg aaaatccgtg cccaggcgat
      541 ggagaaacga taggttttca ggcccatttc cgccatcaag cggatatctt cctccacgcg
      601 atggtaatgg tcgaccgcga catccccgtt tgtgtttttg aaggtggtgc cgggtacttt
      661 cacgaattca tcccagatcg aagggccttt gccgtcctcg ttccatgcgc cttccacttg
      721 ataagctgct gaagcgctac cccagagaaa atccttcggg aaagcattct tgtttgcttg
      781 catcatagtt ttgaacactc ctttttctgc atgatccatt ccaaatgcta tttccactct
      841 attttaacat gcttaatgta gaaaacgcat ggcataaggt agcgtttgca gtaattcagt
      901 ttatttccaa cattttgact gattaaacag acgctatcct ctccggttct tgcgaaaaag
      961 gggttacaat ccagttacac cataaaggag gcgattccat gccaattcat attcatcaac
     1021 aagggaaatt atttcatctg accaatcaga aaatcagcta catcatgcag gtactggagg
     1081 atggcagtct ggggaatcta tatttcggaa aaaaggtagc cgacaggacg gatttcagcc
     1141 atctgatcga atcggcgtat cgcccgaaca cagcctatgc cagccaaaag gactactcgt
     1201 tttcgaggga gcatacacgc caggaatttc cggtctacgg gacgactgat ttccgccatc
     1261 cggccgtcag cgtgctgcac gagaacggca gccgcgtgtc cgctttccaa gtgaaaggac
     1321 acgcggtcca ttcgggtaag aaa
//
LOCUS       DAOMUJ010000514         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26708_length_1342_cov_1.803419, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000514 DAOMUJ010000000
VERSION     DAOMUJ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26708_length_1342_cov_1.803419"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLT73_09935"
     CDS             complement(<1..1123)
                     /locus_tag="PLT73_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRM20727.1"
                     /translation="MKPTKSLSFFNLILIIVMTVYSFSSMSSSFFMMGLKSFPWFLIS
                     AFFYFIPYALIVSEYTRSYSNRTGGIYDWLKDAFSPRIAFITAFLWYCSYFTWIISLF
                     MKLLIPFTIMLFGQDLTSAEQWFGIDTAYWIMVLSLVAVFCMTWVINRGHQAVFSFLK
                     TSSYAMVGLLLISGIGNLMLMVNTPQLIAQNMQQSLAAPSFFKGTNDSFLSQLPFFIF
                     AITAFGGLDTIASLVDKSGQQQKKFPKALIFSAVIIVVLYFGGIMLWSGANNLNVLRE
                     TDQFHLGNLMYGLMGSLANNLSIAFGLSASAQAFLYQAFIRYTAFTLFVAYIGLLSSI
                     TYTPLKSLIQGTPKEIWPQFLTKINHKQMPQTALWIQASV"
BASE COUNT          430 a          263 c          325 g          324 t
ORIGIN      
        1 caaccgaagc ttggatccaa agtgctgttt gtggcatctg tttgtggttg attttagtca
       61 gaaattgcgg ccaaatttcc tttggcgtgc cctggatcaa actttttaat ggtgtatagg
      121 tgatggacga caacaatccg atataggcta caaacaaggt gaaggcagta tagcgtatga
      181 atgcttgata taggaatgcc tgagcagatg ccgacagacc gaaggcaatg ctgaggttat
      241 tggcaagact gcccatcaaa ccgtacatca gattgccgag atggaactga tcggtttccc
      301 gcaatacatt caggttattc gcaccgctcc atagcataat gccgccgaaa taaaggacga
      361 cgatgatcac agcgctgaaa atcaatgctt tcggaaattt cttttgctgt tggccggatt
      421 tgtctacaag actggcaatc gtgtccaatc cgccgaatgc ggtgatggca aaaataaaga
      481 agggcaactg cgaaaggaat gaatcattgg tccctttgaa gaaactcggg gcggcaaggc
      541 tctgctgcat attttgagcg atgagttgcg gagtgttcac catcaacatc agattgccga
      601 ttccagaaat gagcagcaga ccgaccattg catagctgct cgtcttcaaa aatgaaaaga
      661 cggcctggtg accgcggttg atcacccaag tcatgcaaaa tacagctaca agtgagagaa
      721 ccataatcca gtaggccgtg tcgatgccga accattgctc agcggaagtc aagtcctgtc
      781 caaaaagcat gatcgtgaaa ggaatcaaca atttcatgaa gaggctgatg atccacgtga
      841 agtagctgca gtaccacagg aaggcggtaa tgaacgcgat acgcggtgaa aaagcatcct
      901 tcagccaatc atagatgccg cctgtgcgat tcgaatagct gcgtgtatat tctgatacaa
      961 taagggcata gggtataaaa taaaaaaaag cgctgatcaa aaaccaaggg aatgatttca
     1021 gacccatcat gaaaaatgag gacgacatac ttgaaaaaga atatacggtc atgacgatga
     1081 tcagtatcag attgaaaaat gatagacttt tggttggttt cataataaac tccttattaa
     1141 taatttgctt ccaacaattg gtatcatcat agtaactttt tggataaagg gcaacaaaaa
     1201 tccacagatt tcgaaaaata tagggaattg gagtagtgaa taatgtcaca aatagatttg
     1261 tggttgcagg agaaaagaaa agaatggcag gcagattttt taggcttggc tcaggatgtt
     1321 gccctggcct gccaacaaac tg
//
LOCUS       DAOMUJ010000515         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26711_length_1342_cov_1.643357, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000515 DAOMUJ010000000
VERSION     DAOMUJ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26711_length_1342_cov_1.643357"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLT73_09940"
     CDS             <1..764
                     /locus_tag="PLT73_09940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM20728.1"
                     /translation="MGIIAPSQQMKEKLASYNIYKEIRVIPTGVRIPDRIDGIKAKKR
                     EELGISDSELVLLSLSRLSSEKKLDKLVHAFPEVVAAYPSAKLIFVGDGPVRHELETQ
                     VSEMGLTRNVIFVGEVKNEDVSEYYQMADIYINASDSETQGLTYLESIVNGCPVIAKR
                     NDYLSGLIKVDSLGMLFDEDDQIGKTINAYADFYHNSDVATKQEVWDGLMQEISSKAF
                     ADAVLSYYQASIDIYESQPREELKLKVNLLEKIKR"
     gene            884..>1342
                     /locus_tag="PLT73_09945"
     CDS             884..>1342
                     /locus_tag="PLT73_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRM20729.1"
                     /translation="MEKIGFIGTGVMGSSMIKHLLNAGYPVRVYNRTKSRADAVVSEG
                     AVWCDTPAAVTAQSDIVITIVGYPKDVEETYFGESGIFSKADDHHILIDMTTSTPTLA
                     RKIAAFGKESGVRTLDAPVSGGDKGAQNGTLTTMVGGEEGTFDAVKGVLTV"
BASE COUNT          371 a          243 c          365 g          363 t
ORIGIN      
        1 cgatgggcat tattgctcca agtcagcaaa tgaaagagaa attagcttcc tacaacatct
       61 ataaggaaat tcgcgtcatc ccaacgggcg tccggatacc ggatcggatt gacggcatca
      121 aagccaaaaa gcgcgaagaa ctggggattt cggattccga attggtgttg ctttctttga
      181 gtaggctgtc ctcggaaaaa aaactggata aacttgttca cgcctttcct gaggtcgtag
      241 cagcctatcc ttccgcgaag cttatttttg ttggtgacgg accggtgcgt cacgagttgg
      301 agacacaggt gtcggaaatg ggtttgacca gaaatgtcat ttttgtcgga gaagtcaaaa
      361 atgaagatgt gagcgagtat tatcaaatgg cagatattta tataaatgct tcggattcgg
      421 aaacccaagg cttgacctat ctggaatcga tcgtaaacgg ttgtccggtc atcgctaagc
      481 ggaatgacta tttatcaggg ttgattaagg tagacagtct ggggatgttg ttcgacgagg
      541 atgaccaaat cggcaagact atcaacgcct atgcggattt ttatcacaac tcagatgttg
      601 cgacaaagca agaagtatgg gatggactga tgcaggagat ttcttcaaag gcatttgcgg
      661 atgccgtatt gagctattat caggcgagta ttgacatcta tgagtcacag ccgcgtgaag
      721 aattgaaatt aaaagtgaat cttttggaaa aaatcaaacg ttgagtcgga acaccgggaa
      781 acagattgct tggataatct gtttcccggt gtttttttgt ttttatctga gctaagcgta
      841 aggtatactg ttgacatagt gtttagaagg ggcggtttta taaatggaaa aaattggttt
      901 tatcggaaca ggtgtcatgg gctcatccat gatcaaacat cttttgaatg cgggttatcc
      961 agtccgagtc tataatcgga cgaaaagcag ggcggatgct gttgtctctg aaggagcagt
     1021 gtggtgtgac acccctgctg ctgttacggc gcaatcggat attgtcatca cgatagtggg
     1081 ttatccgaaa gatgttgaag agacttattt tggggagagc ggtatctttt cgaaagcgga
     1141 cgaccaccat attctcattg atatgacgac cagtacgccg actttggcca ggaaaatagc
     1201 tgctttcggc aaagaaagcg gggtccgcac gttggatgct ccagtttctg gaggagataa
     1261 gggtgctcaa aatggaacgc tcacaacgat ggtcggtggt gaggaaggaa cgtttgatgc
     1321 cgttaaagga gttttgactg tt
//
LOCUS       DAOMUJ010000516         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26719_length_1342_cov_1.197358, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000516 DAOMUJ010000000
VERSION     DAOMUJ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_1342_cov_1.197358"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1342
                     /locus_tag="PLT73_09950"
     CDS             <1..>1342
                     /locus_tag="PLT73_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012897374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM20730.1"
                     /translation="VTVIQSGGQYQVVIGNHVPDVYAAVNAVGGLNGGGEVAAEDEGP
                     KGSIFNQFIDMISKIFQPILGPLAATGMLKGVAALLIAAGMSTTDGAYVLIQAAGDGF
                     FNFLPIFLAYTASKHFKMNSFTAMAVATAMVYPGMVNPAGVDPLYTLFAGSIFESPIY
                     MTFLGLPVIMMSYASSVVPILLAVFLGSKVENVLKKVIPDVVKLFMVPFTTLLIVVPL
                     TILFVGPLSTWAATLLGAGVSWIYNLSPIVAGAVLGGGWQVFVMFGLHWGLVPIAINN
                     LATQGYDQLLATVLGVSFAQTGAVLAIMLKTKEAKVKQLSIPAFISGLFGVTEPAIYG
                     ITLPMKRPFQLSCIAGAISGILAGIFKLTSYRMGGLGVFSFPSYLDANGTMSFNFWAS
                     LIVCAVGLAAGFLLVFFSKIPVLYGETETATPATNSAITLNTNSVVVEAMKKDLS"
BASE COUNT          311 a          313 c          329 g          389 t
ORIGIN      
        1 cgttacagtc atccaaagcg gcggccagta tcaagtcgtc atcggcaacc acgttcctga
       61 cgtctacgca gcagtcaacg ctgtcggcgg tttgaatggc ggcggtgagg tagctgctga
      121 agacgaaggt ccaaaaggca gcattttcaa tcaattcatc gatatgattt cgaaaatttt
      181 ccaaccaatc ctgggacctt tggcagctac agggatgctg aaaggtgtgg cagcgctttt
      241 gattgctgcg ggtatgtcga ctacggatgg ggcgtatgtc ctgattcaag cagcaggtga
      301 cggattcttc aacttcttgc cgatcttcct ggcttatacc gccagcaaac acttcaaaat
      361 gaacagcttc actgcgatgg cagtcgcaac cgcaatggtc tatccgggca tggtcaatcc
      421 tgctggcgtg gatccattgt acactttatt cgctggcagc atttttgaat caccgatcta
      481 catgaccttc ttgggcttgc cagtcattat gatgagctat gcctcatctg ttgttccaat
      541 tctgctggcc gtctttctgg gttccaaagt tgaaaatgtc ttgaagaaag tcattccgga
      601 tgttgtgaaa ttgttcatgg tgcctttcac aactttgttg atcgtcgttc ccttgaccat
      661 cttgtttgtc ggaccgctct ccacatgggc tgcgacgttg cttggcgctg gtgtatcctg
      721 gatctataac ttgagcccaa tcgtcgcagg cgctgtattg ggtggaggct ggcaagtatt
      781 cgtcatgttc ggtttgcatt ggggcttggt gccgatcgca atcaataact tggcgacaca
      841 aggctatgat caattgttgg ctactgtatt aggcgtttca tttgcacaga ccggtgcagt
      901 attggctatc atgttgaaaa cgaaagaagc aaaagtaaaa caattgagta ttccggcttt
      961 catttccggc ttattcgggg taactgagcc tgctatctac gggattactt taccgatgaa
     1021 acgtccgttc caattgagct gtattgctgg tgccatctcc ggtatcttgg caggtatctt
     1081 caaattgaca agctaccgta tgggcggctt gggcgtcttc tcattcccta gctacttgga
     1141 tgcaaacgga acaatgagct tcaatttttg ggcttccctc atcgtatgtg cagtaggact
     1201 tgctgccgga ttcctgcttg tcttcttctc aaaaatcccg gttctttacg gagaaactga
     1261 aactgcaaca cctgcaacaa attcagcaat aacattgaac acaaactccg tagttgttga
     1321 agcaatgaaa aaagacttga gt
//
LOCUS       DAOMUJ010000517         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26750_length_1341_cov_1.422240, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000517 DAOMUJ010000000
VERSION     DAOMUJ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26750_length_1341_cov_1.422240"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLT73_09955"
     CDS             complement(<1..1080)
                     /locus_tag="PLT73_09955"
                     /inference="COORDINATES: protein motif:HMM:NF037886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRM20731.1"
                     /translation="MPTKKRGNPKSIRWKWGFLLSIAFFLLYFLSALLMLNVYTGYHY
                     DEQKDEAENLLYSVQTALSEIDTKLSVNSVESMFEKISVSTVTKGLQSVGNYPFLEDL
                     KNRGANIRVFDTEGQLLYETQKSYTTFIKNPDTYLRETKLNNLEAFVAGSPILSKEES
                     FLLGYVQIVFRLNDYHTVVSQMKQYFWLVTAIVLVLSAAAGYGIAIYFFRPIKQMVDT
                     MDAIEEDTLSETRIKISKSKDEFTDLSVHINGLLDKMALYVTQQKQFVEDVSHELRTP
                     TAIVEGHLKLLNRWGKEDPQILDESLAASLTEIQRMKTLVQEMLDLSRAEQVEIHYKN
                     EVTPIRNVVLHTFHNFKMLYPDFVFN"
     gene            complement(1103..>1341)
                     /locus_tag="PLT73_09960"
     CDS             complement(1103..>1341)
                     /locus_tag="PLT73_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010011025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM20732.1"
                     /translation="DEHIELTKREYELLLILMENINVVLSRDVLLNKVWGYKTEVETN
                     VVDVYIRYLRNKIDVAGQESYIQTVRGTGYVMRS"
BASE COUNT          357 a          310 c          278 g          396 t
ORIGIN      
        1 attgaaaaca aaatccggat acagcatctt aaagttgtgg aacgtatgca gcacgacatt
       61 ccggatcggc gtaacttcat ttttatagtg gatttctact tgttccgctc ttgaaaggtc
      121 aagcatctcc tggaccaaag tcttcattct ctggatttca gtcaaggagg cggcaaggga
      181 ttcatccagg atttgcgggt cttccttgcc ccaacggttc agtaatttca gatggccctc
      241 aacaatagcc gtaggggtac gcagctcatg cgatacatcc tccacaaact gcttttgctg
      301 agtaacatac aatgccatct tgtccaagag accgtttata tggacagaaa gatcggtaaa
      361 ttcgtcctta cttttcgata ttttaatccg ggtctctgac aaggtatcct cttcgatcgc
      421 atccatcgtg tcgaccatct gtttgatcgg cctgaagaag taaatggcga tgccgtagcc
      481 ggcagctgct gacaaaacaa gcacaatcgc cgtcactaac cagaaatact gtttcatctg
      541 cgagaccaca gtgtgataat cattcaatcg aaaaacgatc tgcacatatc ccaacagaaa
      601 ggactcttcc ttacttagga tcgggctacc tgcgacaaac gcttcaagat tattcagttt
      661 cgtctcgcgt aaataagtat cgggattttt tatgaatgtc gtatagctct tctgtgtttc
      721 atagagcagc tgtccctctg tatcaaatac gcgtatgttc gcccctctgt tcttgagatc
      781 ctccaaaaac gggtaattac cgacagactg aaggcctttc gtcacggtgg aaacagaaat
      841 tttctcgaac atgctttcga cactgttgac ggacaacttc gtgtcaattt cagataaggc
      901 agtttggacc gaatagagca ggttctcggc ctcatctttt tgctcatcgt aatgataacc
      961 tgtatagaca ttcagcatga gtaaagcgga cagaaagtac agcaggaaga aagcgatact
     1021 taaaagaaaa ccccatttcc accgaatgga cttgggattt cctctttttt tggttggcat
     1081 ataattgatt ctgcgcatag tattatgagc gcatgacgta tccggtgcca cgaaccgttt
     1141 ggatataact ttcttggccg gctacatcga ttttgttgcg cagatatcgg atatatacat
     1201 cgactacgtt tgtttccact tctgttttgt atccccatac tttgttcagc aatacatccc
     1261 ttgaaaggac aacgtttatg ttttccatca ggatcaataa taattcgtat tcgcgttttg
     1321 tcaattcgat gtgctcgtcg c
//
LOCUS       DAOMUJ010000518         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26889_length_1334_cov_1.704457, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000518 DAOMUJ010000000
VERSION     DAOMUJ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26889_length_1334_cov_1.704457"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1334)
                     /locus_tag="PLT73_09965"
     CDS             complement(<1..>1334)
                     /locus_tag="PLT73_09965"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HRM20733.1"
                     /translation="DRAIFLLLKQIIDASATSARDYRSSEDRKEITIPASMVKKLLEK
                     LANCPLVFIKTNPYQTQGRALLPEQLVQDFDQLPISFALSELPKGGLLFEETTEVSSE
                     NIFFNQADIFLIDGNFYFLTESMKDRLNSIYTAISQSGHEGLHIPPEAAGDFLAIAVP
                     ALQKLVTISLAESVQSTYQRFPLKAELYLDWKQEKLIMELFLNYGEIKIDAAKEGLAH
                     KDLTPDTVIRDRIAESIMIQTFSDCANNALKFDGLLYLDALDDIMSFLYEGLEKLAPH
                     CGIFTTAAVENLLYQPSRPPKVAIEVNSQSRLLDVTFSTDEINVEDLKQMVRQIAQNK
                     NYHRLSNGKIVNLRQKEIKELTDAVVQLDVPLKDLKKEMSLPLYKAFGVDTNAGVVHQ
                     DEDFMTLINRMERPEKIDFELPEDLTATLRPYQETGYKWLKGLDHYGFGGIL"
BASE COUNT          311 a          337 c          312 g          374 t
ORIGIN      
        1 aaggatgccg ccgaagccgt agtgatcgag cccctttagc catttgtatc ctgtttcctg
       61 atacgggcgc aaagtcgctg tcaaatcctc aggcagttcg aagtcgattt tttcgggcct
      121 ctccatccga ttgatgagcg tcatgaaatc ctcatcctgg tggacgaccc ccgcattggt
      181 atccacaccg aacgccttat agagcggcag tgacatctct tttttcagat ccttcaaagg
      241 tacgtccagc tgcacgaccg catcggtcag ctccttgatt tccttttggc gcaaattaac
      301 gattttcccg tttgataggc ggtgatagtt tttgttctgg gcgatctgcc tgaccatctg
      361 tttcaggtcc tccacattga tttcatcggt gctgaacgtc acatccaaaa gacgtgactg
      421 gctgttgact tcgatggcga ctttcggcgg cctgctgggt tggtaaagca ggttctcaac
      481 ggctgctgtc gtgaatattc cgcagtgcgg agctagtttt tccaggcctt cgtaaaggaa
      541 cgacatgatg tcgtccaggg catccagata gagcaatcca tcaaacttta acgcattatt
      601 tgcacaatcg ctgaacgttt ggatcatgat gctttcggca atccgatccc tgatgaccgt
      661 atccggagtc aaatctttat gggccaagcc ttcttttgcg gcatcaatct ttatttcccc
      721 gtaattgaga aacaattcca tgatcagctt ttcctgtttc cagtccagat aaagttccgc
      781 cttcaatggg aagcgttggt aggtcgattg cacggattcc gctagcgaga tggtcacaag
      841 cttctgcaaa gccggcacag ctatcgccaa gaaatcaccc gcggcttccg gtgggatatg
      901 caatccctca tgcccggatt gtgaaatggc tgtatagata ctgtttaaac ggtccttcat
      961 gctttcggtc aaaaagtaga aatttccgtc aatcaggaag atgtccgcct ggttgaaaaa
     1021 aatattctcg gatgaaactt ccgttgtttc ttcaaagaga agccctccct tcggcaattc
     1081 actcagcgcg aaactgatcg gaagctgatc aaaatcctgc accagttgtt ccggcaacaa
     1141 cgctctccct tgggtctggt agggatttgt tttgatgaaa acaagcggac aattcgccag
     1201 cttttccaat aatttcttca ccatgctcgc cggaattgtg atttctttgc gatcctcgct
     1261 cgatcgatag tcgcgagcgg atgtggccga agcatcgatg atttgcttca aaagaagaaa
     1321 aattgcacgg tctt
//
LOCUS       DAOMUJ010000519         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26910_length_1333_cov_1.789515, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000519 DAOMUJ010000000
VERSION     DAOMUJ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26910_length_1333_cov_1.789515"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>427
                     /locus_tag="PLT73_09970"
     CDS             <1..>427
                     /locus_tag="PLT73_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRM20734.1"
                     /translation="PAGMEEEHVTSLQSLIDELEHLPETRLIVVGGAGSLYADPGKTI
                     RVMETANFPEAFKPTAKNMAKALSLLKESKVNWTYLSPSAYFDPNGNRTGKYAEGAET
                     LLLNSEGESYISYADYAIALVDEIERQRHLRQRYTVVGER"
     assembly_gap    428..437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            509..1255
                     /locus_tag="PLT73_09975"
     CDS             509..1255
                     /locus_tag="PLT73_09975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM20735.1"
                     /translation="METLDLIIDFHKNNPRQGPGSQQSSIKALQFLPQLTPQAQLLDV
                     GCGTGAQTMVLSEQTPAHITAVDSSKEFLSILKHKALQQGITNRLTVKEMDMEQLAFA
                     PESVDVIWAEGAIYNIGFSRGIREWRPLLKKDGYLVVSEISWLTPQRPQIVDKYWTEV
                     YPEMGTIAEKIAQVEDAGYIPVAHFVLPESDWTSHYYHYYETNEAAFLERHGHSEDAK
                     ALVEENRQEMKHFEKYSAYYNYVFYIMQKR"
BASE COUNT          382 a          328 c          351 g          262 t
ORIGIN      
        1 cctgcgggaa tggaagagga gcatgtgacc tcgctgcagt ccttgatcga tgaactggag
       61 catctgccgg aaacacgctt gatcgttgta ggcggagccg gcagccttta cgcagatccc
      121 ggaaaaacga tccgggtgat ggaaacggcc aattttcccg aggctttcaa accgactgcg
      181 aaaaacatgg ccaaggccct cagcctactg aaggaaagca aggtcaattg gacatatctt
      241 tcgccatccg cttattttga cccgaacggc aaccggaccg gaaaatacgc tgaaggggca
      301 gaaacgctgc tgctgaacag cgaaggcgag agttatatca gttacgcgga ttatgcgatc
      361 gcactggtgg acgaaatcga aaggcaacgg catctgagac aacgctacac agtcgtcggc
      421 gaacggcnnn nnnnnnnaaa aaatacaaaa ttgaacgtct ggcagagcta aactttgaca
      481 gtacgaaagc agatcaggag gggaattcat ggaaacattg gatttgatca tcgattttca
      541 taaaaacaac ccgcgccaag gccccggcag ccaacagagc tccatcaaag ccctgcagtt
      601 tctgccgcaa ttgactccgc aagcacaact gctggacgtc ggctgcggta ccggtgcgca
      661 gacaatggtg ctgagtgaac agacgcctgc ccacatcaca gcggtcgaca gcagcaaaga
      721 atttttgagc atactcaaac acaaagccct gcaacaaggc atcacgaacc ggttgacggt
      781 caaggaaatg gacatggaac agctggcttt tgcacccgaa tcagtggacg tcatctgggc
      841 ggaaggcgcc atctacaaca ttggtttttc cagaggcatc cgggaatggc gtccgttgct
      901 gaaaaaagac ggttacttgg tcgtttccga aatttcctgg ctgacgccgc aacgtccgca
      961 gattgtggac aaatattgga ccgaagtcta tccggaaatg gggacgattg cggaaaagat
     1021 tgcccaagtg gaggatgccg gctacatccc ggtggctcat ttcgtcctgc ccgaatcgga
     1081 ctggacgagc cattactatc attactacga gaccaatgaa gcggcattcc tggaaaggca
     1141 cggccacagc gaggacgcaa aagctttggt ggaagagaac cgtcaggaaa tgaaacattt
     1201 cgaaaaatac agtgcgtact acaactatgt tttctacatc atgcagaaac gttgaaaaac
     1261 tgaaaaatag aggcgccctg tccatccaac ggacagggcg cctcgttttt ttatcaatag
     1321 gtcagtcagt ttt
//
LOCUS       DAOMUJ010000520         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_26928_length_1332_cov_2.429131, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000520 DAOMUJ010000000
VERSION     DAOMUJ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26928_length_1332_cov_2.429131"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLT73_09980"
     CDS             <1..342
                     /locus_tag="PLT73_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20736.1"
                     /translation="RGPEEPQVVSFSRTITIPYLPATVSESASESNNGQGSENANSNA
                     NVSSTSNEPNHIVIYVQDEDHTLSDVFREFDISADEVVTINFRILEGSSASYRIERDG
                     EVISETSELTQ"
     gene            448..>1332
                     /gene="rsgA"
                     /locus_tag="PLT73_09985"
     CDS             448..>1332
                     /gene="rsgA"
                     /locus_tag="PLT73_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRM20737.1"
                     /translation="MQKGQVRKAISGFYYVYVDGQTYQTRGRGNFRVKNMAPLIGDFV
                     DFESDNLTEGVLLDIYPRKNELVRPTVANVDCGVIVMSAIEPDFSSYLVDRFLVYLEA
                     NNISPIIYVTKIDLLDDKAKADMLQYKTYYEAIGYTMILSDYPDTSSLDELVQTMGKG
                     MAVFMGQSGAGKSTLLNQLMPELDLLTGEISTALGRGRHTTRHVELHPVGEALIADTP
                     GFSTIDLIDIEAVDLPSFFPDFEALRAQCRFAGCMHINEPNCRVKEAVASGEVAPYRY
                     EHYLQFHEEIVGRKPMYIK"
BASE COUNT          375 a          281 c          334 g          342 t
ORIGIN      
        1 agaggccctg aagaaccaca agtggtttcc ttcagccgta ccatcacaat tccctacctt
       61 ccggcaaccg tatcggaatc cgcttctgag agcaacaatg gtcaaggtag tgaaaatgcc
      121 aacagcaatg cgaatgtcag cagtacttcc aacgagccga accatatagt catttatgtg
      181 caggacgaag atcatacact cagtgatgta ttccgtgagt tcgatatttc agcagatgaa
      241 gttgtgacaa taaacttccg cattctggaa ggcagctctg cttcctaccg gatcgaaagg
      301 gatggcgaag tcatcagcga aacttctgaa ttgacgcaat agaattgtat aggctacaaa
      361 aaatgacaaa catcaaaatg gtttgtcatt tttttgtaga acagtgtaga atagagagaa
      421 agaatcaaac taggaggcgt tgtcgatttg caaaaaggac aagtcaggaa agcaataagc
      481 ggattttatt atgtgtatgt ggatggtcaa acgtatcaga cgagagggag aggaaacttc
      541 cgcgtaaaaa acatggctcc gcttattggg gattttgtcg atttcgaaag tgacaactta
      601 acggagggcg ttctgttgga tatctacccc aggaaaaatg aattggttcg tccgacggtg
      661 gcgaatgtcg attgcggtgt cattgtgatg tcggcgatcg aacccgattt ttcttcctat
      721 ttggtggatc gtttcttggt ttacctggaa gcaaataaca tcagtcccat catctacgtt
      781 acaaaaatag atctgttgga cgacaaggct aaagctgaca tgcttcagta taaaacctat
      841 tatgaagcaa tcggctatac gatgattcta tcggattatc cggatacatc ttctttggat
      901 gaactggtcc agaccatggg taaagggatg gctgttttta tgggccaatc aggcgccggg
      961 aaatcaactt tgttgaacca actgatgccc gaattggatt tgttgacagg tgaaatctcg
     1021 accgctttgg gcaggggccg ccatacgacg cggcatgtcg agttgcatcc ggttggggaa
     1081 gcgctgattg ccgatacccc tggtttcagt acaatcgact tgatcgacat tgaagcggtc
     1141 gatctgcctt cttttttccc ggactttgaa gcgctgcgcg ctcaatgccg attcgccggc
     1201 tgcatgcaca tcaacgagcc gaattgccgg gtgaaagagg ctgttgcatc cggagaagtg
     1261 gctccgtaca gatacgaaca ctatcttcag ttccatgagg aaattgtagg ccgcaaaccg
     1321 atgtacataa aa
//
LOCUS       DAOMUJ010000521         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27231_length_1321_cov_1.325434, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000521 DAOMUJ010000000
VERSION     DAOMUJ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27231_length_1321_cov_1.325434"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLT73_09990"
     CDS             <1..454
                     /locus_tag="PLT73_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM20738.1"
                     /translation="SYLGGKKIRIITNSYPVFENLRQYDTADIIMIGGDYRKNTGAFF
                     GPIANDNLKKLKFSKCFISANGIHNEAISTYSMAEGETQNIALNNSRTKYLLADNKKF
                     NRDDFYNFYNLHDIDHLVTDDHISADLVTHYSQYVTLTQAQTEEGANL"
     gene            457..1143
                     /locus_tag="PLT73_09995"
     CDS             457..1143
                     /locus_tag="PLT73_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM20739.1"
                     /translation="MNGIIFDFNGTMFQDSHLHEQAWIDMIQKYSPGNGLTETEILTN
                     VHGRTNDKILRHFVSPDLTDEEVQKLSLEKEEHYRRLCLNKPEQLVLTDGLTEVLDDL
                     KERKLPLTIATATIKENVDFYFDTFALDRWFDPDKVVFDDGSFPGKPEPDIFLHAARK
                     LGVPPEECLVIEDAYSGLRAANSANIGMIIAIDPFFKNRETFQKENLCKDGVITDFHG
                     FTQRLFAEQA"
BASE COUNT          363 a          369 c          315 g          274 t
ORIGIN      
        1 gagctatctc ggcggcaaaa aaatccgcat catcacaaac agctatccgg ttttcgaaaa
       61 tctgcgccaa tacgacaccg cggatatcat tatgatcggt ggcgattacc ggaaaaacac
      121 gggtgctttc ttcggcccga tcgcgaacga caacctgaaa aaattgaagt tctccaaatg
      181 cttcatcagc gccaacggca tccacaatga agcgatctcc acctatagca tggcggaagg
      241 agaaacccag aatatcgccc tgaacaactc ccgcacaaaa tacttgctgg cggacaacaa
      301 gaaattcaac cgagacgact tctacaattt ctacaacctg cacgacatcg atcacctggt
      361 caccgatgac catatcagcg cagatctggt cacgcattac tcgcaatacg tcaccctgac
      421 ccaagcccaa acagaagaag gagctaactt atgaaactga acggcatcat ttttgacttc
      481 aacggcacaa tgttccagga ttcgcatctg catgaacagg cttggatcga catgatccaa
      541 aagtacagtc caggtaacgg gctcacagaa acagagattc tgacaaacgt acacggccgc
      601 acgaacgaca aaatcctgcg tcattttgta tcccctgact tgacggacga agaagtgcag
      661 aaactttctt tggagaagga agagcattac cgcagactct gcctgaacaa accggagcaa
      721 ctcgtattga cggacggctt gaccgaagtg ctggacgatc tgaaggagcg caagttgccg
      781 ctgacgatcg ccaccgcgac catcaaagaa aatgttgact tctatttcga cacctttgcg
      841 cttgatcgtt ggttcgatcc ggacaaagtc gtcttcgatg atggttcctt ccccggcaaa
      901 ccggagccgg acatcttcct gcatgccgcc agaaaactcg gggtcccccc cgaagaatgt
      961 ctggtcattg aggacgccta ttccgggctc cgcgcggcca acagcgcgaa catcggtatg
     1021 atcatcgcaa tcgacccgtt cttcaagaac cgggaaacct tccaaaagga aaacctctgc
     1081 aaggacggtg tcatcacgga cttccacggc ttcacccagc gccttttcgc agagcaagca
     1141 taaaaattaa agagaacaca ccatcggccc ggccgatggt gcgttttctt tttggtaaag
     1201 tacgttgttg ccaaggttac cgtatcaacg ggagctgtgc tccggtgaac gacggcttcg
     1261 ccgtagttga ctgctgggtt tcgctgtcct gctccggcga agggggattc gccggcgttg
     1321 g
//
LOCUS       DAOMUJ010000522         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27246_length_1320_cov_1.254545, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000522 DAOMUJ010000000
VERSION     DAOMUJ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27246_length_1320_cov_1.254545"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..928)
                     /gene="mtfA"
                     /locus_tag="PLT73_10000"
     CDS             complement(131..928)
                     /gene="mtfA"
                     /locus_tag="PLT73_10000"
                     /inference="COORDINATES: protein motif:HMM:NF011939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HRM20740.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDPFSATNVH"
BASE COUNT          378 a          310 c          277 g          355 t
ORIGIN      
        1 ccgccgttct accgactgaa ctacagagga atcgtgtgaa cggggcgcat attacttagc
       61 ggtaccttgt ctgtcaacac taaatttcat aagtcatttc aattggttaa ttaatctgca
      121 aagttgttaa ttaatgaaca ttcgtcgccg aaaacgggtc tgtatcatta gcgtgatgca
      181 gtctctgcaa aggatcttgt tgataaaatt gacagaaacg ttgccacaat gaagggaaac
      241 gaggagcaaa aagttctggg gcgctaaaga aatattcaga aagtacggca aaacattcag
      301 caggatcact ggcagcataa gcatcaatgc tcgccgcatt ctcgccaacc aattcgattt
      361 cttcctgaat gttgttcatt gcagcatgaa gatcgtgttc ccagccagca acctcacgca
      421 acggaataaa gggaactccg ctggcgcgat cgccgttacg ggtgtccagc ttatgagcga
      481 cttcatgaat aatcaggtta aaaccagaag catcaaaaga atcttgtata tccaaccagt
      541 tcaaaacgat aggcccttgc tgccagctct gacctgactg aacaatacgt tggttatgca
      601 ccagaccgat atcgtcttcc cattcatcat cgaccacaaa tggcgcagga taaattaaga
      661 cttcatgaaa accatccagc cattccagtc ctaactccag aacgggtagg caaaatagaa
      721 gtgctatccg gcagcttctt aatgaatcca gttcaaagcc ctgtaaagga acaagccgct
      781 tttgctgtaa aaaacgttcg gcaagagtga ctaatttgct ttgttcctgt tccgtcagac
      841 ccgttaaaag ggggatcgat agtgcttcct gccagggaag ggcagtttga tgtgctgatt
      901 cttgtacttt ccagggccac ttaatcatcg ttttgctcgc aaactcgtca cttgaacaaa
      961 attgcacgga cagggactgt taaaatgcca aatttcctgg catcatggca acagaaatac
     1021 aggtaactat ctgtttttaa ataaaagcag ttcggtctcg aaaaccggag taggggcaac
     1081 tctaccgggg gttcaaatcc ccctctctcc gccactttat caatgactta tctcccgact
     1141 tcccgccttg cttttcctaa acagaacaat cgtagaatat tcttgaaggg ttagatcgtc
     1201 actgttttct gttcgatact gtgacattca gcacttgatt cctatggatc tgacaggaag
     1261 ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca ttattctttt
//
LOCUS       DAOMUJ010000523         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27316_length_1317_cov_2.587956, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000523 DAOMUJ010000000
VERSION     DAOMUJ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27316_length_1317_cov_2.587956"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLT73_10005"
     CDS             complement(<1..844)
                     /locus_tag="PLT73_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20741.1"
                     /translation="MYDTTGQKKILEVKNLKQYFNVGTKNEVRAVDDVSFDIYEGETL
                     GLVGESGCGKTTTGRAIIRLYNPTSGEIFFDGTEIHTLHDRKEQLAFRKDMQMIFQDP
                     YASLNPRMKVRDIVAEGLKIHGLATTEKEVDDRVAELLDLVGLNKDHSSRYPHEFSGG
                     QRQRIGIARALAVNPKLIIADEPISALDVSIQAQVVNLLMELQKKQKLTFLFIAHDLS
                     MVKYISNRIGVMYFGKLVELAPADDVYNKPLHPYTESLLSAIPLPDPVYERNRTRFTY
                     VPREE"
     gene            complement(844..>1317)
                     /locus_tag="PLT73_10010"
     CDS             complement(844..>1317)
                     /locus_tag="PLT73_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20742.1"
                     /translation="VAVMYAGKIVEVGTVDEIFYNPQHPYTWGLLSSMPTLDTQGTLY
                     AIPGTPPDLLDPPNGDAFAPRSEYAMKIDTLYDPPFFKVSDTHAAATWLLHPDAPSVN
                     PPEGIMNRRATFSKMNLAPSDELKVMDTADKDPAVLPHGKDSDIDSKITEKGGLL"
BASE COUNT          337 a          310 c          300 g          370 t
ORIGIN      
        1 tctcttcacg gggaacataa gtgaagcgcg tgcggttgcg ctcataaacc ggatccggca
       61 gcggtatggc cgataacagg ctttccgtat aaggatgtaa aggtttgttg taaacgtcgt
      121 ctgcgggcgc taattcgacc aatttgccga agtacataac accgatacgg ttgctgatgt
      181 acttgaccat cgatagatca tgcgcgatga acaagaaagt cagcttttgt tttttctgca
      241 attccatcag caagttgact acttgggcct gaatggatac gtccaaagcg gaaatgggct
      301 catcggcaat gatgagtttt ggatttacag ctaaggctct ggcgattccg atacgctgtc
      361 tttggccacc tgagaactca tgcggataac gtgaggaatg atctttgttc aatccaacca
      421 aatccaacaa ttccgcaacc cgatcatcaa cttccttctc ggtagtcgca agtccatgaa
      481 tttttaatcc ttccgcaacg atatcgcgga ctttcatacg ggggttcaaa gatgcgtaag
      541 gatcttggaa aatcatttgc atgtcttttc ggaaagccaa ttgctctttt ctatcatgta
      601 gcgtatggat ttcggtaccg tcaaaaaata tttcaccgga cgtagggttg tacaaacgga
      661 tgattgcacg accagttgtc gtttttccac aaccggattc acctaccaag cccagtgttt
      721 caccttcata tatgtcaaag gaaacatcat caacagctcg gacttcattc tttgtgccga
      781 cattgaaata ctgcttcaaa ttttttactt ccagaatctt tttctgtcct gttgtgtcat
      841 acattacagc agacctcctt tctcggttat tttagaatcg atgtccgagt ctttgccatg
      901 aggcaaaaca gccggatctt tatcggcggt gtccatcact ttcaattcat ccgatggcgc
      961 aaggttcatt tttgagaaag ttgcacgtcg attcatgatg ccctctggcg gattcacgga
     1021 cggtgcgtcg ggatgcaaca gccaggttgc agccgcatgg gtatcggata ccttaaagaa
     1081 cggcggatcg tacaaagtat cgattttcat cgcatactcg ctccggggag caaaagcatc
     1141 accatttgga ggatccaaca aatcaggcgg tgttccaggg atcgcatata gcgttccttg
     1201 cgtatccagt gtaggcattg agctcaatag accccatgta tacggatgtt gcggattata
     1261 gaaaatttca tccacagtcc ctacttcgac gattttgccg gcatacatga cggcaac
//
LOCUS       DAOMUJ010000524         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27365_length_1316_cov_1.908803, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000524 DAOMUJ010000000
VERSION     DAOMUJ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27365_length_1316_cov_1.908803"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLT73_10015"
     CDS             complement(<1..385)
                     /locus_tag="PLT73_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRM20743.1"
                     /translation="MKILFAASEAAPFFKTGGLGDVAYALPKELVKQGVDIRVVLPYY
                     STMPAKYKADIKELLHFRVQVGWKSMYCGVKTLELDGVTYYFIDNQAYFDRPSLYGQM
                     DDAERFGFFSLAICEMMEKIDFIPDV"
     gene            complement(403..>852)
                     /locus_tag="PLT73_10020"
     CDS             complement(403..>852)
                     /locus_tag="PLT73_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HRM20744.1"
                     /translation="MPEYQINTYEYTGYMANIDSIPAYFNANMEMLEKNKFSSLFHGS
                     QNVITKVKNGAPTYYSKEAHVKNAQFATGCFVEGTVEDSLIFRKVNIAKGAEVRKSII
                     MQGAEIGEGAILEYCILDKNVTIGPGVTLKGTKDNIVVIEKNKTLTV"
     assembly_gap    853..867
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<868..1242)
                     /locus_tag="PLT73_10025"
     CDS             complement(<868..1242)
                     /locus_tag="PLT73_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20745.1"
                     /translation="MDYYMNHKEFIARSKGEYVIVMGSKILANVDIKAIMQHHLAKDG
                     ALTVVYKTVPRSFLENRPDEKVLKIDRDEYLVRLTDAKDAPEDLERLAMSMNMYFMRA
                     DKMLELIQRAEAEDVQLDSGKLI"
BASE COUNT          327 a          304 c          269 g          401 t
ORIGIN      
        1 cgacatccgg gatgaaatca attttttcca tcatttcgca gatcgccagc gagaagaagc
       61 cgaacctttc ggcatcatcc atttggccgt acaaggacgg acgatcgaaa taggcttgat
      121 tatcgatgaa ataataggtt acgccatcta attccaaagt tttcacgccg caatacatgc
      181 ttttccaacc gacttgcaca cggaaatgca gcaattcctt gatatctgct ttgtattttg
      241 caggcatcgt cgaatagtac ggaagcacca ccctgatatc cacgccttgt ttgactaact
      301 ccttcggcag tgcgtacgcg acatcgccga gcccgcctgt tttgaagaaa ggagcagcct
      361 cacttgcggc aaataatatt ttcatcgtgc gcatccttct ttctaaactg tgagtgtttt
      421 atttttttcg atcactacaa tattatcttt cgtgcctttc aaagtcacgc cgggcccgat
      481 ggtcacattc ttgtccagga tacaatattc aagaattgcg ccctcaccga tttctgcacc
      541 ctgcatgatg attgatttgc ggacttcggc acccttcgcg atgttgactt tgcggaaaat
      601 caaggaatcc tcgacagtcc cttcaacaaa acagcctgtt gcaaattggg cattctttac
      661 atgggcctct ttagaataat aagtcggtgc cccgtttttc actttagtga tgacgttttg
      721 gcttccgtgg aacaaagatg agaatttgtt cttttccagc atttccatat tcgcattgaa
      781 gtaggcaggg atagaatcga tattggccat atagcctgtg tattcgtaag tattgatttg
      841 gtattcaggc atnnnnnnnn nnnnnnngat cagtttgccc gaatccagtt gcacatcctc
      901 cgcttcggca cgttggatca attcgagcat tttgtccgct ctcatgaagt acatgttcat
      961 tgacattgcc agacgttcga gatcctcagg tgcatctttg gcatccgtca agcgcaccaa
     1021 atattcatca cgatcgatct tcaaaacttt ttcatccggt ctgttttcaa ggaaggatct
     1081 aggaacggtc ttgtagacta ccgttagagc gccatccttt gccagatgat gctgcatgat
     1141 ggctttgatg tcgacattcg ccaagatctt gctgcccatc acgatgacgt attcgccttt
     1201 tgaacgtgcg atgaattctt tgtggttcat atagtaatcc atatcgtgta atggtttatt
     1261 tagtataaaa tgttccaaat aggtttctat atagttaaaa gaagctatta ctgaaa
//
LOCUS       DAOMUJ010000525         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27623_length_1306_cov_1.848122, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000525 DAOMUJ010000000
VERSION     DAOMUJ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27623_length_1306_cov_1.848122"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLT73_10030"
     CDS             complement(<1..503)
                     /locus_tag="PLT73_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM20746.1"
                     /translation="MGYTHKTIEKKWQKYWADNRTFKTTEAGGKPKYYVLDMFPYPSG
                     QGLHVGHPEGYTATDIVARAKRAQGFNVLHPMGWDAFGLPAEQYALDTGNDPADFTEL
                     NIKTFKRQIQSLGFSYDWSREINTTDPSYYKWTQWIFTKLYEQGLAYEAEVSVNWCPA
                     LGTVLAN"
     misc_binding    complement(592..798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(887..>1306)
                     /locus_tag="PLT73_10035"
     CDS             complement(887..>1306)
                     /locus_tag="PLT73_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM20747.1"
                     /translation="KVTKSVTNGNNSTGVTPPTVTKPSVPTVVNKVYTVQKGDTLYKI
                     ASANGVSVAELKAWNNLNTDIIFVNQSLKLAATTSSTQTTAPTTSAPEASGTTYTVQK
                     GDTLYSIAKKKGVSLAALIEANDMTSNVIYVGQVITF"
BASE COUNT          310 a          323 c          280 g          393 t
ORIGIN      
        1 tcgttggcaa ggacggtccc tagcgccgga caccagttga cggaaacttc cgcctcatag
       61 gccaaacctt gttcgtacaa ttttgtgaag atccattgcg tccatttgta gtagcttggg
      121 tccgtcgtat tgatttcacg gctccagtca taactgaacc ctagcgattg gatctggcgc
      181 ttgaaagttt tgatgttaag ctcggtaaaa tcagccggat cgttaccagt atccaacgca
      241 tattgctcag ccggcaatcc gaaagcatcc caacccatcg gatgcaatac attgaaacct
      301 tgtgcgcgtt ttgcgcgggc aacgatatcc gtcgccgtgt agccttccgg gtggcctaca
      361 tgcaagcctt ggccggatgg gtaagggaac atatccaata cataatattt cggtttcccg
      421 cccgcttcgg tcgttttgaa ggtgcggttg tctgcccaat atttctgcca ttttttctcg
      481 atcgttttat gcgtataacc catttccagc aacatccttt tcttttcatt catccaaaca
      541 ggcgatggcc ataaaaaaag tcctataggc aacgtccatc actggttctg cctataggac
      601 gaaatgttcg cggtaccacc tatattgaat gtctttgcat tccttatcat gcctgtaacg
      661 cagcgatgcg gtcccgctta ctgatgcttt cggcggaaca actcaaaggc gagttcaaaa
      721 ggctctttgt gcattttcac cacccatgca ctctcttcaa aaaagctgct ctctactatt
      781 ccttctctat gtctgtttca gttatgaatt gattcatctg ctattttaac aaataaaact
      841 catttcatca agacctgatg ccttcaagcg tgattcagtt gattacttag aaagtgatga
      901 cttggccgac ataaatgacg ttggaagtca tatcattcgc ttcgatcaaa gctgctaagc
      961 taacaccctt tttcttggcg atgctgtaca aagtgtcgcc tttttggaca gtataagtag
     1021 tgccggatgc ctcaggcgca cttgtggtag gcgctgttgt ctgcgttgaa ctcgtcgttg
     1081 cagccaactt caaggattgg ttcacaaaaa tgatatccgt gttcaggttg ttccaagcct
     1141 tcaattcagc aaccgaaaca ccattagcgg aggctatttt ataaagcgtg tccccttttt
     1201 ggaccgtata cactttattc acgactgtcg gaacagaagg cttcgtcaca gtcggtggcg
     1261 ttacgcctgt ggaattattc ccattcgtaa ctgattttgt tacttt
//
LOCUS       DAOMUJ010000526         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27627_length_1306_cov_1.645883, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000526 DAOMUJ010000000
VERSION     DAOMUJ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27627_length_1306_cov_1.645883"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..837
                     /locus_tag="PLT73_10040"
     CDS             73..837
                     /locus_tag="PLT73_10040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM20748.1"
                     /translation="MTNKLALITGASSGIGQEFAKKLSQQGYDLILVARREDRLLEIA
                     KHLSTKSEIITADLAKAEECYRLYEETKGKNISMLINCAGFGDFGAFTTTSLDKELEM
                     IDVNIKAVHILTKKFLPDMIAKNEGYILNVASVAGLMPAGPYMATYYATKAYVTSFTS
                     AIAEELEEKKSNVYVGSLCPGPVDTEFNKVADVKFNLKGITSEYCVNYALDKMYKRKK
                     IIVPTMLLKGALFSSKLAPRNLVVRLTSRQQKKKQG"
BASE COUNT          381 a          289 c          347 g          289 t
ORIGIN      
        1 atattgctct gatgaagcaa atacgatacg ctaaatacaa ctatcatttt gacgaaacgc
       61 gagggacaaa taatgacaaa taaactagcg ctcatcactg gcgcaagctc cggtatcggt
      121 caggagttcg ctaagaagct gagccaacaa gggtacgatc tgattctggt tgcgaggcgt
      181 gaggatcgac ttttggaaat cgccaaacat ttgtcgacca aaagtgaaat catcacagcg
      241 gacttggcta aggcggagga atgctaccgt ctgtacgaag aaacgaaggg caaaaatatt
      301 tccatgctga tcaattgtgc cggtttcggc gatttcggcg cgttcacgac gaccagcctg
      361 gacaaggaac tggagatgat cgatgtcaac atcaaggcgg ttcatattct gaccaagaaa
      421 tttctgccgg atatgatcgc aaaaaatgaa ggttatattc tgaatgttgc ttcggttgcc
      481 ggactgatgc cagcggggcc ttacatggca acttattacg caaccaaagc ctacgtaaca
      541 agtttcacct ctgcaatcgc tgaagaattg gaagagaaga agagcaacgt ttacgtcggt
      601 tcgttatgtc cggggccggt agacacagag ttcaataaag tagctgatgt gaagttcaat
      661 ctgaagggca tcacttctga atactgcgtc aattatgcct tggataaaat gtataaacgc
      721 aagaaaatca tcgttccgac gatgttgctg aagggagcgc tcttctcgtc gaagctggcg
      781 ccgcgcaact tggtcgtgcg cctgacgagc aggcagcaaa agaaaaaaca aggataatga
      841 atgcgggagg ccagctggcg caagcgaagc tggccgaaag aaaccgaact ggattcagct
      901 gtttttctcc ggtgagttct gatacggccg gaggagacgc ttaaatgggg ctcctgaact
      961 caggtgaagc aatcttcacc tgaggaggca acccgaaacc aagttttcct ccggcgaagc
     1021 tcctcttggc cggagaacac gagcaaaaat gatagccgaa tttccgccgt agccctgtaa
     1081 gcagaaaaaa ataacgtcca agcgaccagt gtaacgctgc tggccgcttg gacgttattt
     1141 atcgtgcttc atcgagaagc cagcagacta cggcctcccg tttggtgcct aagcgttttc
     1201 ttggtaaaat agatacaaac gggtggtgga acaaaatcag ctgaaaaagc cgctgcctta
     1261 tcccagatta gaaagcgtgc cggtgaagag agggggtttg atgatg
//
LOCUS       DAOMUJ010000527         1304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27686_length_1304_cov_1.321857, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000527 DAOMUJ010000000
VERSION     DAOMUJ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27686_length_1304_cov_1.321857"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..534
                     /locus_tag="PLT73_10045"
     CDS             97..534
                     /locus_tag="PLT73_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4869 domain-containing protein"
                     /protein_id="HRM20749.1"
                     /translation="MLSVYFGDMPEAIYNTATYFKNSYRSSWITDPYAVSIIKDVDRS
                     DVVSENVIESPVLGSISPLQLSGGVKTLLLMRFDRKQIFNASTCGDNCAKWILDMAKD
                     RKLVVNLYHVMDFGREDFKIKVVNSGRIVHNMTELIHESIPYL"
     gene            749..>1087
                     /locus_tag="PLT73_10050"
     CDS             749..>1087
                     /locus_tag="PLT73_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20750.1"
                     /translation="MIGETSFRPFMTRLYLSMRAYSDEFAHHVLYSSNYFVLISRADF
                     PNLSYSVDEIYKIKTSGKYHSFIPVYQDRGNHRYAISRSAPKQDFSILLCEDSKSGFQ
                     FFERHFADSEL"
     assembly_gap    1088..1097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1098..>1304
                     /locus_tag="PLT73_10055"
     CDS             <1098..>1304
                     /locus_tag="PLT73_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HRM20751.1"
                     /translation="NSAILGWLDQHFDDRVFVVADGAAFGCYADRVLKLQDIHRDTVT
                     VCLPESFEWLLLSSGVILGLDVKAV"
BASE COUNT          288 a          287 c          326 g          393 t
ORIGIN      
        1 agcgccttct tgctttcttt taggggccac cgttatttgg tggccccttt cgttatgatt
       61 acggtgacca tatagccaaa atgattgggg gtggatgtgt taagcgtcta ctttggcgat
      121 atgccagaag cgatttacaa cacagcgaca tatttcaaaa actcttaccg gtcttcttgg
      181 attacggatc cctatgcagt ttcgataatt aaagatgtcg atcgatcgga tgttgtctcc
      241 gaaaacgtca tcgaaagccc tgtgcttgga tccatctccc cactccagct ttctggtggc
      301 gtgaaaacgc tgctgcttat gagatttgat cgtaagcaaa tttttaacgc ttctacctgt
      361 ggtgacaact gtgccaagtg gattctcgac atggcgaaag accgcaagct cgttgtgaat
      421 ctctatcatg tgatggactt tggtcgcgag gattttaaaa ttaaagtcgt gaatagcggc
      481 cgaatcgttc acaacatgac cgaattgatt cacgagtcaa ttccgtatct gtaggtgctg
      541 cttatgaacg gttcgcatct tgttaagatt tcccgccgta ggggaaccaa gtacacattt
      601 accatcaagc ggaacatcac tattgtgcgt ggcgatagtg gcacgggtaa gacgacactg
      661 tttgacatgg tcgcagacta catgcgaacg ggcgagcagt cgggtgtttc cttgcaatgc
      721 gattgtccct gtgtcgccct gaccgattat gattggcgaa accagctttc gtccgttcat
      781 gactcgattg tatttgtcga tgagggctta ttctgatgag tttgcccatc atgtgctgta
      841 ttcctcgaat tatttcgtgc tgatctcaag ggctgatttc cccaatcttt cgtatagcgt
      901 ggatgagatt tacaagatta agacgagcgg aaaataccat tctttcatcc ctgtttatca
      961 agatcgcggg aatcatcgtt atgctatttc ccggtcggcg ccaaaacagg acttttctat
     1021 ccttctatgc gaggacagta agtctggttt ccagttcttt gaacggcatt tcgctgacag
     1081 tgagcttnnn nnnnnnnaac agcgcgattt tgggctggtt ggatcagcat ttcgacgatc
     1141 gcgtgtttgt tgtcgcggac ggagcggcat ttgggtgcta tgcggaccgc gtccttaagc
     1201 tgcaagacat tcatcgcgat actgttacgg tttgtcttcc cgagtcattc gagtggcttc
     1261 tgctgagctc cggcgttatt ttagggttag atgttaaggc ggtt
//
LOCUS       DAOMUJ010000528         1301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27750_length_1301_cov_1.901284, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000528 DAOMUJ010000000
VERSION     DAOMUJ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27750_length_1301_cov_1.901284"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLT73_10060"
     CDS             <1..198
                     /locus_tag="PLT73_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20752.1"
                     /translation="TPKSYESCETFLTAKTIAHRYIVVNKYPFALKRAGTATDGEDLK
                     IQLQQKTPLMAYSGYGEFFGQ"
     gene            complement(220..>1301)
                     /locus_tag="PLT73_10065"
     CDS             complement(220..>1301)
                     /locus_tag="PLT73_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HRM20753.1"
                     /translation="VTSPANLIASITPDWMTIGIITMLIFAYYLISTILPIDKAMGKV
                     YPWFGAILIISTIAIGISLLTGGHNLPELTMGAMQNFHPKGTPIFPAIFFTISCGAIS
                     GFHATQAPMVSRTSTNEREGRFLFYGMMIAEGVIAMIWAGASMALFDGQTLSQMIDAG
                     TPSAVVNQVSTMLLGNVFGTVAIIGVIVLPISSGLSAFRSLRTILADYMNIKQDSMKK
                     ILMMTIPLFVISFFLTKVDFNLIWRYFNWANQVTAVIALLMSTRYLYLKNKNYLVTLL
                     PATFMLYACVVYILSEPIGFRMGLQTATYLVGLVATVAIMALYWTTGVKQKVALSPES
                     ELLNDHLPIGTFSSIDAVPAAIVAE"
BASE COUNT          374 a          304 c          324 g          299 t
ORIGIN      
        1 acccctaaat catatgaaag ttgtgaaacg tttcttacag ccaaaactat agcacaccga
       61 tatattgtcg tcaacaagta tccctttgcc ctgaaaaggg caggcaccgc aactgatgga
      121 gaagatttga aaatccaact acagcaaaaa actcccctga tggcctatag cggctacggg
      181 gagttttttg gtcaataagg tttcaattga aagatagggc tattcggcta ctatcgctgc
      241 cggtactgcg tcaatagaag aaaacgtccc gatcggcaga tgatcgttca agagttccga
      301 ttcaggactc aaagcaacct tctgtttcac gccggttgtc cagtaaagtg ccatgatcgc
      361 aacagttgca accagaccga ccaagtaagt ggccgtctgc agccccatcc ggaatccgat
      421 cggctcgctc aggatataga cgacacaggc ataaagcatg aatgttgcag gcaataaggt
      481 aaccaggtaa tttttatttt taagatacaa gtaacgcgtc gacatcagca aagcgatgac
      541 ggctgtaact tggttcgccc agttgaagta tctccagatc aggttgaaat ccactttagt
      601 aaggaagaac gagatgacaa acaacggaat cgtcatcatc aggatttttt tcattgaatc
      661 ctgtttgatg ttcatgtagt cagccaaaat tgtccgcaga cttctgaatg ctgaaagtcc
      721 tgaagagatc ggcaagacga tgacgccgat gatggcgacc gtaccgaaga cattacccaa
      781 taacatagtg gaaacttggt tgacgacagc tgatggtgtt ccggcatcga tcatttggct
      841 caatgtttga ccatcgaaca aggccatgga cgctccagcc cagatcatag cgatgacgcc
      901 ctctgcgatc atcatgccgt agaaaaggaa gcggccttcc cgctcgttcg tcgacgtccg
      961 ggaaaccatc ggcgcttggg tcgcatggaa accggaaatg gcgccacagg aaatcgtaaa
     1021 gaagatggcc gggaaaatcg gtgtgccttt cggatggaaa ttctgcatag cgcccatcgt
     1081 caattccggc aagttatggc cgcccgtcaa caagctgatg ccgattgcga tcgtactgat
     1141 gatcagaatt gcaccgaacc aaggatagac ttttcccatc gccttgtcga tcggcaggat
     1201 ggttgagatg agatagtaag cgaaaatcag catggtgatg atgccgattg tcatccaatc
     1261 aggcgtaatg ctggcgatca gattggcggg tgaagtgaca a
//
LOCUS       DAOMUJ010000529         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27808_length_1298_cov_2.056315, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000529 DAOMUJ010000000
VERSION     DAOMUJ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27808_length_1298_cov_2.056315"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLT73_10070"
     CDS             complement(<1..399)
                     /locus_tag="PLT73_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897453.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRM20754.1"
                     /translation="MSKKPVAIIILDGFGWRNEMMGNAVKLAKKPNFDRYWNNFPHAT
                     MLASGLAVGLPEGQMGNSEVGHTNIGAGRVVYQSLTRIDKAIQDGEFLTNEALVGAYD
                     HTTENDSALHLFGLLSDGGVHSHINHVIALI"
     gene            complement(399..1154)
                     /gene="tpiA"
                     /locus_tag="PLT73_10075"
     CDS             complement(399..1154)
                     /gene="tpiA"
                     /locus_tag="PLT73_10075"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRM20755.1"
                     /translation="MRKPIIAGNWKMNKTAKEAQAFIEAVKTKVPAFDKVEAVIGSPA
                     LFLENMVAATEGTDLKVAAQNCYFEEEGAFTGEISPKALGDLGVDYVIIGHSERREYF
                     HETDEDINKKAHAILRNGMTPIVCCGETLEQRESGVTNEWVSGQITAALKNLTAEQVA
                     NLVIAYEPIWAIGTGKSSSAQDANDTCGVVRATVATIFGQEVADKVRVQYGGSVKPEN
                     IAEYMAEEHIDGALVGGASLVPDSFLALLEAIK"
     gene            complement(1181..>1298)
                     /gene="pgk"
                     /locus_tag="PLT73_10080"
     CDS             complement(1181..>1298)
                     /gene="pgk"
                     /locus_tag="PLT73_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM20756.1"
                     /translation="AMQLGFADDFTHISTGGGASLEYLEGKELPGVASISDK"
BASE COUNT          333 a          319 c          259 g          387 t
ORIGIN      
        1 gatcaaagcg atgacatggt tgatgtgact gtgcacacca ccgtcggaaa gcagaccgaa
       61 aagatgcaaa gcagaatcat tttcagtcgt gtgatcgtaa gcaccgacaa gcgcttcgtt
      121 cgtcaagaat tcgccatctt ggatagcttt gtcgattctt gtcaaacttt ggtagacaac
      181 tcgtccggca ccaatgtttg tatgccctac ttcagaatta cccatttggc cttcaggaag
      241 tcctacagcc aatccggaag ccagcatcgt tgcatgcgga aaattgttcc aataacggtc
      301 aaagttcggt ttctttgcta gcttaaccgc attgcccatc atttcatttc tccagccgaa
      361 accgtccaaa ataattattg ctaccggttt tttactcatt atttaatagc ctccaataat
      421 gctaagaatg aatcaggaac caaactagca ccaccgacca acgcgccgtc aatatgctct
      481 tcagccatgt attctgcaat attttcaggt tttacgctgc cgccgtattg aacacggact
      541 ttgtcagcga cttcttgtcc aaagattgta gcaacagttg cacgcactac gccgcaagtg
      601 tcgttcgcat cttgtgcgga agaagattta cctgtaccga ttgcccagat tggctcataa
      661 gcgataacca ggttagccac ttgttctgca gtcaggtttt tcagagctgc agtaatttgt
      721 cctgaaaccc attcgtttgt tacgcctgat tcgcgttgtt ccaacgtttc accacagcaa
      781 acgataggtg tcataccatt gcgcagaata gcatgcgctt ttttgttgat gtcttcatct
      841 gtttcgtgga agtactcacg tctttcagag tgtccgataa taacgtagtc gacgcctaag
      901 tcgcctaaag ctttcgggct gatttcgcca gtgaaggcac cctcttcttc aaagtaacag
      961 ttttgcgcgg caaccttcaa atcagttccc tctgtagctg ctaccatgtt ttctaaaaat
     1021 aaagccggtg atccaataac agcttccact ttgtcaaaag caggaacctt agtttttaca
     1081 gcttcgatga atgcttgtgc ttccttagct gttttgttca ttttccagtt accggcaata
     1141 atcggtttac gcaatataat cactccttaa gataggttgc ttatttgtca gaaattgatg
     1201 ctacacctgg taattcttta ccttcaaggt attccaatga tgctccgcca cctgttgaga
     1261 tgtgcgtgaa atcatcagca aagcctaatt gcatagcc
//
LOCUS       DAOMUJ010000530         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27812_length_1298_cov_1.850362, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000530 DAOMUJ010000000
VERSION     DAOMUJ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27812_length_1298_cov_1.850362"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLT73_10085"
     CDS             complement(<1..299)
                     /locus_tag="PLT73_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20757.1"
                     /translation="MNTNNIEKQNSHLNLQDQAVQDIFLLYQDYPEVPYISEKRDKEG
                     WLSAVRIGSEQLVPKRNMIRFEEDILPGHLILLWRIQFGTFTNENVYPKYFEYNYG"
     gene            complement(327..689)
                     /locus_tag="PLT73_10090"
     CDS             complement(327..689)
                     /locus_tag="PLT73_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20758.1"
                     /translation="MEERNTLLINQSTIIFPLGSFQEIEDELILKADKIIVDHVDQAL
                     HRGALKKLTGEGKLSENNIFATLGELAVGKKDAGDLSQGITLCIPIGTGAMDVAIAGE
                     VYRRALEKGMGYAFDFEA"
     gene            893..>1298
                     /locus_tag="PLT73_10095"
     CDS             893..>1298
                     /locus_tag="PLT73_10095"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRM20759.1"
                     /translation="MEKKSEKAKVRKPELVFYAGSTLLEGPHWDAQNNLIYCVSIEDN
                     MIYAIDPETGEVGTYLTEGPVGCAVLDQKGMILEAEKSGIFRIDPRTKEKQLVAKAHD
                     DERIRYNDGKLDPKGRLLVGTMGYDKTYEGESA"
BASE COUNT          360 a          283 c          308 g          347 t
ORIGIN      
        1 ccgtaattgt attcgaaata tttcggatag acgttctcgt tcgtgaaggt gccaaactga
       61 atgcgccaca acaagatcaa gtgtccaggt aggatatctt cttcaaaacg gatcatattg
      121 cgttttggga caagctgttc tgacccgatc cggacggcac tgagccaacc ttccttgtcg
      181 cgtttttcgg agatgtaagg cacttccgga taatcctgat agagcaagaa aatatcctgc
      241 accgcttgat cctgcaagtt caagtggctg ttttgttttt cgatgttgtt ggtattcata
      301 aaaatccctc ctactcgttc ctgtttttat gcttcaaaat caaaggcgta acccattcct
      361 ttttcgagtg ctcttcggta aacttctccg gcgatcgcaa catccatcgc tccggtgccg
      421 atcgggatgc agagggtgat gccctgtgaa agatcgcctg catctttctt tccgaccgct
      481 aattcgccca acgtcgcaaa aatattattt tcggataatt ttccttcacc tgtcagtttt
      541 tttaaagcac cgcgatggag agcttggtcc acatggtcaa cgatgatctt gtccgccttc
      601 aggatgagtt catcctcgat ttcctggaaa gatcccaagg gaaaaatgat ggtgctctga
      661 ttgatcaaaa gcgtattgcg ttcttccatg gccattctcc tttaaacgag atgtgtacaa
      721 aagtatcttt ccatttggac agtcacataa agtatagcat ttttcggagc agaacaaaaa
      781 atcgcaagca ccggatgtca aaaactatcc ataaaatcat atcaattgga aagcattttt
      841 tattggtata tactgtgtgt aagcatgaca caggaatatg aggtgaagaa atatggaaaa
      901 gaaatccgaa aaggctaagg tgagaaaacc ggaactggtt ttctatgctg gatcgacatt
      961 gttggaagga ccgcactggg atgcgcaaaa caatctgatt tattgtgttt ccatcgaaga
     1021 caacatgatc tacgccatcg atcctgaaac tggggaggtg gggacctact taactgaggg
     1081 gccagtcgga tgcgccgttc tcgatcagaa gggcatgatt ctggaagctg aaaaaagcgg
     1141 tattttccgg atcgatccgc ggacgaaaga aaagcaactg gtggcaaagg cacacgacga
     1201 tgaacggata cgctacaacg acgggaagtt ggatccgaaa ggccgcctgc ttgtcgggac
     1261 gatgggctat gacaaaacct atgaagggga atccgctt
//
LOCUS       DAOMUJ010000531         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27813_length_1298_cov_1.844730, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000531 DAOMUJ010000000
VERSION     DAOMUJ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27813_length_1298_cov_1.844730"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT73_10100"
     CDS             <1..283
                     /locus_tag="PLT73_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM20760.1"
                     /translation="VRKAGHFTEYLILAILFLRTRKERGSSGRSALYALLFVFLYASS
                     DEFHQSFIPGRGPAFTDVLIDTAGGLTGIILFEWKQSILEKQNRIFCKK"
     gene            complement(345..1043)
                     /locus_tag="PLT73_10105"
     CDS             complement(345..1043)
                     /locus_tag="PLT73_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRM20761.1"
                     /translation="MAKRKTSGKHKLLRLLLLFAWFLILTWIEIHEGVDPTSNQDGTT
                     TTAVILSTDDAPQYTGPDFLEINGGKSTFDDSELRFEGEGWIRYGDLDELNRVTTMNA
                     MITPEMYQTGTDANSSILPTGWSKSNQNDSNPKQLNRGHLLADVLGGSGEDWRNLVTL
                     YRNANYPAMYFASEYIVSEAIQSGTTVRYRVTPLFKDNELICEGLHIMAKSVDDDSVD
                     LNVYVFNEPKDAVD"
BASE COUNT          311 a          313 c          296 g          378 t
ORIGIN      
        1 agtgcgtaag gctggccatt tcaccgaata tttgattttg gctatcctgt ttttgcgtac
       61 tcggaaagag cggggatcat cgggaagatc agctttgtat gcgttgctgt tcgtattttt
      121 gtacgcctcc tcggacgagt tccatcaatc cttcattccg ggaagaggtc ctgcatttac
      181 cgatgtattg atcgataccg cgggaggatt gaccggcatc atcctttttg aatggaagca
      241 gagcattctg gaaaaacaaa atcggatctt ctgcaaaaaa taaacaggca tccatctatt
      301 tgatggtgcc tgttttaaaa ttatggaaaa gcccagtcgc tgacctaatc gacggcatct
      361 ttgggctcgt tgaagacata gacgttcaga tcgactgaat cgtcatccac gctttttgcc
      421 atgatgtgca gcccttcgca aatcaattca ttgtctttga agaggggcgt tacccgatag
      481 cggactgttg tgccagattg gatcgcttca ctgacaatgt attccgaggc gaaatacatg
      541 gccggatagt tcgcgttgcg gtacaacgtc accagattgc gccagtcctc accgcttcct
      601 cctagcacat ccgccagcaa atgcccgcgg ttcagctgtt ttggattcga atcattctga
      661 ttgcttttcg accagccagt cggcaggatg ctgctgtttg catcggttcc cgtctggtac
      721 atttccggcg tgatcatggc gttcattgtg gtgacgcggt tcagctcatc caaatcgccg
      781 taacggatcc agccttcccc ttcaaagcgc aattctgaat catcaaaggt gctcttccca
      841 ccgtttattt cgaggaagtc cgggccagta tattgaggcg catcatccgt ggacaggatt
      901 actgcagttg tggttgtgcc gtcctggttt gaagtcggat cgactccttc atgtatctca
      961 atccaggtaa ggatgagaaa ccaggcaaac agcaaaagca gcctcaacag tttgtgcttg
     1021 cctgaagttt ttctttttgc cattttttcc cccacagata atatttgaaa gcattccttc
     1081 attctatcag tcaaccattg ggagtgcaaa cagatttctg tcaataatcg aacccgtttt
     1141 ctttgtctga gcagattgta tttttgagaa caaaaaaact cttctcaaat tacaagaaga
     1201 gtcggtaagg caaaattatt cagttactgg ctcatttttg acacttttga cactcgcccg
     1261 gatatcgaat ttccctatcg gatccggccc acgcatga
//
LOCUS       DAOMUJ010000532         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_27915_length_1294_cov_2.096852, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000532 DAOMUJ010000000
VERSION     DAOMUJ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27915_length_1294_cov_2.096852"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /gene="mtnN"
                     /locus_tag="PLT73_10110"
     CDS             complement(<1..227)
                     /gene="mtnN"
                     /locus_tag="PLT73_10110"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRM20762.1"
                     /translation="MIGIIGAMEEENRILLENMAEKTEETHFHLVFTRGKIENQEVVL
                     VQSGIGKVNSAIATAFIIDRYRPDFVINTGSA"
     gene            complement(261..638)
                     /locus_tag="PLT73_10115"
     CDS             complement(261..638)
                     /locus_tag="PLT73_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20763.1"
                     /translation="MPEPKKPLITRERYRQYKRDQTKPLVTDEKKHTPATEKNNKQST
                     ASAGEKSSIPAEKKRSENGNKAKTAKPKAKLFRKADKPAASTKVERLSDKERGRKLDG
                     YLNKAILIVILLIILVFAIAFVL"
     gene            complement(640..1200)
                     /locus_tag="PLT73_10120"
     CDS             complement(640..1200)
                     /locus_tag="PLT73_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM20764.1"
                     /translation="MEFEEKTLKSEKIFDGVLLHLVREEVLLPNGKTSVREMIRHPGA
                     VAMIPFTSDGKMVMVRQFRKPLDRTVVEIPAGKLETSDAEPLLAAIRELEEETDYRAE
                     KWSELTVFYPTPAYLDEKITIYLAEGLTKVENSLPMDEDEFIEILELTYDEAKALQES
                     GEICDSKTIYAMLFWEMRLLRERIEG"
BASE COUNT          284 a          331 c          257 g          422 t
ORIGIN      
        1 gcggaaccgg tattgatgac gaagtcggga cggtagcgat cgatgataaa agcggtagct
       61 atagccgaat tgactttacc gataccggat tgcaccagca ccacttcctg gttttcgatt
      121 ttccctcttg taaaaacaag gtggaaatgc gtttcctctg ttttttctgc catattttcc
      181 aacaaaatac ggttttcttc ttccattgct ccaataattc cgatcatgag tgtttccttc
      241 cttgtatctg cattgttatg ttataggaca aaggctatcg caaaaaccaa aatgatcaac
      301 aagatgacga tcaggatggc tttgttcaga tagccgtcca gctttcggcc tctttctttg
      361 tccgacaaac gctccacctt tgttgaggct gccggtttgt cggccttccg gaacagcttc
      421 gcttttggct tggcggtttt ggctttattg ccgttttccg aacgtttctt ttcagccgga
      481 atactgcttt tctcgcccgc agaagcagtt gattgcttgt tgtttttttc cgttgccgga
      541 gtgtgtttct tttcgtcggt caccagcggt ttagtctggt cccttttata ctgacgatag
      601 cgttccctgg tgatcagcgg tttctttggt tctggcatgc tatccctcta ttctttcccg
      661 caaaagacgc atttcccaga acagcatggc atagattgtt ttcgaatcgc agatttctcc
      721 ggattcctgc aaagctttcg cttcatcgta ggtcaattcc aatatttcga tgaattcatc
      781 ttcgtccata ggcaaagaat tttcgacttt cgtcaagcct tccgccaaat agattgtgat
      841 tttttcatcc aaataagccg gtgtcgggta aaaaaccgtc aattcagacc atttttctgc
      901 gcgataatcg gtctcttcct ccagctcacg gatcgctgcc agcaaaggtt ccgcatcgga
      961 agtctccaat ttgcctgccg gtatttcgac aacggtccga tccaacggtt tccggaactg
     1021 tctgaccatg accattttcc cgtcggaagt aaatggtatc atcgctaccg cacccggatg
     1081 tctgatcatc tctctgactg aagtttttcc gttcggcagc agcacttctt ccctaactaa
     1141 atgcaacaag acaccgtcga atattttttc gcttttcaat gttttttctt caaattccat
     1201 ttctgtacct cccaatccac tttcctcatc atctaatatc ataacctatc ttttccgcca
     1261 tttacaagag ccgcgagaaa ttcaggctaa atgg
//
LOCUS       DAOMUJ010000533         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28142_length_1286_cov_1.642567, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000533 DAOMUJ010000000
VERSION     DAOMUJ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28142_length_1286_cov_1.642567"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLT73_10125"
     CDS             complement(<1..1056)
                     /locus_tag="PLT73_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRM20765.1"
                     /translation="MGKITGFKEYSRSNYEKISPEVRIKNWGEMREETSEEKIRTQAS
                     RCMSCGIPFCSAGIMLPNGASGCPLHNLIPEWNDMVYRGQWQEAYERLSLTNPFPEFT
                     GRVCPAPCEGSCTVGLIGEPVTISSIEYEIIEHAFQNGWVKPAKAPATGKRVAVVGSG
                     PAGLATAHYLTSVGHAVTVFERSDRPGGLLMYGIPNMKLDKDIIQRRLDILEEAGVTF
                     VCNTEIGKDIAAQELVDTYDAVVLCGGATRARGINVEGNDAKGVHMAMDFLTANTQNI
                     LAGDPQESKITAKDKHVIVIGGGDTGTDCVGTSIRHGAKSVHQFEIMPQAPDKRDANT
                     NPWPEWPRVCKTDYGQEE"
     gene            complement(1071..>1286)
                     /locus_tag="PLT73_10130"
     CDS             complement(1071..>1286)
                     /locus_tag="PLT73_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20766.1"
                     /translation="MLQEHMIFTGSPKAQNILANWDQMKSRFTKVISNDYKFILENIE
                     IANQMGISGEDAMRYAFDERYKPVTTN"
BASE COUNT          277 a          332 c          315 g          362 t
ORIGIN      
        1 ttcttcctga ccgtagtctg tcttgcagac tctaggccat tctggccatg ggttcgtgtt
       61 tgcatcacgt ttgtccggag cttgcggcat aatttcgaat tgatgcacgc tcttcgcgcc
      121 gtgacggatg gacgtaccga cacagtcggt cccggtatcc ccgccaccga taacgatgac
      181 gtgtttgtct ttggcggtga ttttactttc ctgcggatcg cctgcaagga tattttgtgt
      241 gttcgcggtc aggaagtcca tggccatgtg tacgcctttt gcatcattgc cttcgacatt
      301 gatgccgcgc gcgcgggttg caccgccgca aagaacgact gcatcatagg tatcaaccaa
      361 ttcctgagct gcgatgtctt tgccgatttc agtgttgcag acaaacgtaa cgccagcttc
      421 ttccaggatg tccaatcttc tttggatgat atccttgtcc aatttcatgt tcgggatacc
      481 gtacatcagc aaaccacccg gacggtcgct tctttcgaag acagtgaccg catggccgac
      541 tgaagtcaag tagtgcgcag tcgcaaggcc ggcaggacct gagccgacaa ccgcaacgcg
      601 tttgccggta gccggagctt tagccggttt tacccaacca ttctggaaag catgttcgat
      661 gatttcgtat tcgatgctgc tgatcgtgac gggttcaccg attagcccga ctgtacaaga
      721 tccttcgcaa ggagcaggac agacacgtcc tgtaaattcc ggaaatggat tggtcaagga
      781 taaacgttcg taagcttctt gccattgacc gcgatacacc atgtcattcc actcggggat
      841 gagattgtgc agcgggcaac cggaagcacc gttcggcagc atgatgcctg ccgaacagaa
      901 gggaatgcca caactcatac aacgggacgc ttgtgtgcga attttttctt cggatgtttc
      961 ttcacgcatt tcgccccaat ttttgatacg gacttccgga gaaatctttt cataattgga
     1021 acggctatat tccttaaatc cagtaatttt tcccatctta tccctcctcc ttagtttgtc
     1081 gtcactggtt tatagcgctc atcaaaggca taacgcattg cgtcttcacc gctgatgccc
     1141 atctggttgg ctatttcgat gttctccagg atgaacttgt agtcgttgga gatgaccttc
     1201 gtgaagcggg atttcatttg atcccagtta gccaaaatat tttgggcttt aggacttcct
     1261 gtaaagatca tgtgctcctg cagcat
//
LOCUS       DAOMUJ010000534         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28261_length_1282_cov_1.656072, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000534 DAOMUJ010000000
VERSION     DAOMUJ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28261_length_1282_cov_1.656072"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..>1282
                     /locus_tag="PLT73_10135"
     CDS             132..>1282
                     /locus_tag="PLT73_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRM20767.1"
                     /translation="MKKTYAIVDLETTGSSFKRGDRIIQFGCTLLQADEIIQEINITI
                     NPGRKIPDIIEKLTGIKNKDVKEAPYFEDVCDFIYNTLEGCIFVAHNVYFDYHFLDSS
                     LREVGMPPLSLPAMDTVELTKILYPTLSSYRLGDLSNYFVLTHDNAHDAASDAHATAE
                     LFLQLKKKANNLPLVTLEKLNFLSAHCQVDNDSFFHESYETAKEIRSPLPQQIYVKNG
                     LALKEKEILSEQTAYREKEVLEIRAEQLEMMQSLFSFHQAETALNLAIEAAPGIGKTL
                     AYLLPAAYQATPKNKVLISTSTLLLQEQIMDDEITRLRKMLPFELQVSSLKGKTNIIH
                     LEKLAALALGSLTKRESLIMMSVFVWLTETESGDISELSQAQNFDFLWE"
BASE COUNT          390 a          275 c          290 g          327 t
ORIGIN      
        1 gttaagctgc atacaaagca cgactcgatc ggaaaggctc gtgtttatat caagagatat
       61 tatcagggaa agtcttgcag aaagaaatac cggaacagga gaagcttctg tttatttagg
      121 agattatttt tatgaagaaa acctatgcca ttgtcgatct tgagacaacg ggatccagct
      181 tcaaacgagg ggatcggatc attcaattcg gatgcacctt gcttcaggcc gacgaaataa
      241 ttcaggaaat caatataacc atcaatcccg gcaggaaaat accggatatc attgagaagt
      301 tgaccggaat caagaataag gatgtaaaag aggcccctta ttttgaagat gtatgtgact
      361 tcatctacaa cacgttggag ggttgcatct ttgtggcgca taacgtatat tttgattacc
      421 attttttgga cagttctctt cgggaagtcg gtatgccgcc gctctcgctg cccgcaatgg
      481 atacagtaga gttgacgaag atcctttatc caaccttgtc cagctatcgg ttaggagact
      541 tatcgaacta cttcgttctg acgcatgata atgcccacga tgccgcaagc gatgctcacg
      601 ccactgcgga actcttttta caattgaaaa aaaaggccaa taacttaccg ttggtcacac
      661 tggaaaaatt aaattttttg tcagcgcatt gtcaggtgga caacgactcc tttttccatg
      721 aaagttatga gaccgccaaa gagatacgct ctccgctccc gcagcaaatc tacgtcaaaa
      781 atggattagc gttaaaggaa aaggaaatac tctcggaaca gactgcttac agagaaaaag
      841 aagtgctgga aattcgtgcg gagcaactag agatgatgca atcacttttt agcttccacc
      901 aggcagaaac agccctaaat ctggcgatcg aagctgcgcc tggcatcgga aaaaccttgg
      961 cctatctatt gcctgcagct tatcaggcta caccaaaaaa caaagtgctc atcagtacct
     1021 cgacgttgct tttgcaagaa cagatcatgg atgacgaaat aacccgtttg cgcaaaatgc
     1081 tgcccttcga gttgcaggtc agctcattga agggtaagac gaatatcatc catctggaga
     1141 aattagctgc tttggcttta gggtctctca ccaaaagaga atcgttgatc atgatgagtg
     1201 tctttgtctg gttgaccgaa acggaatccg gcgatatcag cgaattgagc caagcacaga
     1261 attttgactt cttatgggaa aa
//
LOCUS       DAOMUJ010000535         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28613_length_1268_cov_2.135202, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000535 DAOMUJ010000000
VERSION     DAOMUJ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28613_length_1268_cov_2.135202"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLT73_10140"
     CDS             <1..342
                     /locus_tag="PLT73_10140"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRM20768.1"
                     /translation="NSPWNPLDLSMGSSQSFTKQNVEIEVGYPIAKMLPPQDDIRMSI
                     IPAGKRISCLNIGPYNEIPKIYQEMDQWLAVHDFETNGISYETYYNGGGFTPQEYLTK
                     IELPIQEADEP"
     gene            419..>1268
                     /locus_tag="PLT73_10145"
     CDS             419..>1268
                     /locus_tag="PLT73_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM20769.1"
                     /translation="MQQNKQPAMAENKMGVMPVNKLLITMSVPMMISMLVQALYNVVD
                     SIFVAQIGEKALTAVSLAFPVQNLMIALAVGTGVGINALVSRRLGEKNYAAANSTADN
                     GIFLNAMHFLLFLALGLFFIPLYFSFQTKDAEIIAYGIDYLQVISVFSFGIFMQVSME
                     RLLQSTGRTLYTMTTQATGAILNIILDPIFIFGWFGVPAMGTRGAAIATVIGQIVAAS
                     LAFFFNVRFNKEITLSFKGFRPDMQTIKKIYSIGGPSILMQAVGSFLNLSLNNILIRF
                     TPTATAV"
BASE COUNT          339 a          306 c          302 g          321 t
ORIGIN      
        1 aacagccctt ggaaccccct ggatttatcc atggggagca gtcagtcatt cacgaagcaa
       61 aacgtcgaaa tcgaagtcgg ctatccgatc gcaaaaatgt tgcctccgca agatgacatc
      121 cgtatgtcca tcattccggc agggaaacgg atatcctgtc tgaacatcgg tccttataat
      181 gaaattccca agatttatca ggaaatggac cagtggttag cggtacatga tttcgaaacg
      241 aacggcatct cctatgaaac ctactacaac ggcggaggct tcacaccgca agaatacttg
      301 accaaaatcg aattgcctat ccaagaggca gatgaaccat aagagtcaag aaaaaattat
      361 agctacaaaa aggcgagccg ttgaatagac ggctccactt ttttggaaag gaaacattat
      421 gcaacaaaac aaacagccag cgatggcaga aaacaagatg ggggtcatgc ccgtcaacaa
      481 attactgatt acgatgtctg tgccgatgat gatctcgatg ctggtgcagg ccttatataa
      541 tgtcgtcgac agcatctttg tggcccagat cggagaaaaa gccttgacgg cagtatcctt
      601 ggcgtttccg gttcagaact tgatgatcgc tttggcggtc gggactgggg tcgggatcaa
      661 tgccttggtt tccagacgcc tgggcgaaaa aaattacgcg gccgcaaaca gtacggccga
      721 taacggcatc tttctgaatg ccatgcattt cctgttgttt ttggcattgg gattgttttt
      781 catcccgctg tatttctcct tccagacgaa ggatgcagaa atcatcgcct acggaatcga
      841 ttatctgcaa gtcatcagtg tcttctcgtt cgggatcttt atgcaggtca gcatggaacg
      901 cttgctgcag tcgacgggca gaaccttgta cacgatgacg acccaagcta caggcgcgat
      961 cctgaacatc atcttggatc cgatcttcat cttcggctgg ttcggtgttc cggcaatggg
     1021 gacaaggggc gctgcaatcg caactgtgat cgggcagata gtcgctgctt cattagcctt
     1081 ctttttcaac gttcgtttca acaaggaaat cactttatcg tttaaaggat tccgtcctga
     1141 tatgcaaacg atcaaaaaaa tctattccat tggcggcccg tcgattctga tgcaagctgt
     1201 cggctccttc ctgaatttga gcctgaacaa tattctgatc cgcttcacac cgacagcgac
     1261 tgctgttt
//
LOCUS       DAOMUJ010000536         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28649_length_1267_cov_1.967822, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000536 DAOMUJ010000000
VERSION     DAOMUJ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28649_length_1267_cov_1.967822"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLT73_10150"
     CDS             <1..577
                     /locus_tag="PLT73_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM20770.1"
                     /translation="LTAGFNRRFAPMVERLKEIPDKKMILIQKDRVKNVEPVRFAVYD
                     LFIHIADTALYLLDDEVVSVQSHLVENEGELKRLWLMLETKSTTCFVSMNYEAGANQE
                     VMEVQSPEGITRVLNLTDMTIVQKNDRQQVAFGDWEGTLRKRGFAPLIAAFVAGVKEK
                     TNPVSTESSYLSHSLCEKILADNGFGSKPNE"
     gene            684..>1267
                     /locus_tag="PLT73_10155"
     CDS             684..>1267
                     /locus_tag="PLT73_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM20771.1"
                     /translation="MTKDMTAGSPAKLLLLFAIPMLIGNIFQQVYSLADTVIIGRTLG
                     VEGLAAIGSVGGVLFFIHGFGIGITSGLGIVIAQRFGTKNEERIRKSITISVWITLFM
                     TVLITIVSVLFAEEILLLMDTPAEIRKEAEAYFLVLMWGSVAIMAFNLLSNILRSLGD
                     SKLPLVILVISSLLNVVLDYVFIVGFSMGVAGAAL"
BASE COUNT          324 a          248 c          345 g          350 t
ORIGIN      
        1 gcttaccgct ggcttcaatc gccggtttgc tccgatggtg gaacggctga aagagattcc
       61 tgacaaaaag atgatcctga ttcagaagga tcgtgtaaag aatgtagaac cggtccgttt
      121 tgccgtttat gatttgttca tccatatcgc ggataccgct ttgtatttgt tggatgatga
      181 agtagtttcg gtgcaatcgc atctggttga aaacgagggg gaactgaaac ggctatggct
      241 gatgctcgag acgaagagta ccacttgttt cgtttcgatg aactatgaag cgggagccaa
      301 tcaggaagtc atggaagtcc aaagtccgga ggggattacg cgtgtcctga atttgactga
      361 tatgaccatt gtacagaaaa atgacaggca gcaagttgct tttggcgatt gggaagggac
      421 actccgaaaa agagggtttg cacctttaat cgctgcattt gttgcaggag taaaagaaaa
      481 aacaaatcct gtctccacgg aatcaagcta tctgagccac tcgttgtgcg agaaaatact
      541 cgccgacaat ggattcggaa gcaagccaaa cgaataggtc ggaatttttg taggtacaat
      601 ttatattttt ttattaattt gttcgtacat ctctggtata atataaatca gcaaggccaa
      661 ggatcatcaa atggaggtga aggatgacaa aagatatgac tgccggcagt cctgcgaagc
      721 tactcttact gttcgcgatt ccgatgctga tcgggaatat tttccaacaa gtgtacagtt
      781 tagccgacac agtcatcatc ggcaggactt tgggagtgga gggattggcg gccatcggat
      841 cggtcggcgg ggtgctcttt tttatccatg gattcgggat tgggatcact tctggtttgg
      901 ggatcgtcat cgcgcagcgt tttggcacga aaaatgagga gcggatccgg aaaagcatca
      961 caataagcgt ctggattacc cttttcatga ctgtccttat cacaatcgtg agcgtgctgt
     1021 tcgcagaaga gattctactg ctcatggata cgccggcaga aatacgcaag gaagccgaag
     1081 cttacttttt ggtcctgatg tggggttcgg tggcgatcat ggcattcaat ttgttgagca
     1141 acatcctgcg ttcactgggt gacagcaaac ttccattggt catcctggtg atttcttccc
     1201 tattgaatgt ggtattggat tatgtgttca ttgtcgggtt ttctatgggg gtcgcaggag
     1261 cggcgct
//
LOCUS       DAOMUJ010000537         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28685_length_1266_cov_1.795211, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000537 DAOMUJ010000000
VERSION     DAOMUJ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28685_length_1266_cov_1.795211"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>646
                     /gene="fliI"
                     /locus_tag="PLT73_10160"
     CDS             <1..>646
                     /gene="fliI"
                     /locus_tag="PLT73_10160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016609002.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HRM20772.1"
                     /translation="IGEVCEIQIKSTGTTVLSEVVGFVDATVLLMPLDEIDGIGPGCL
                     VRPTGKTLQIEVSDELLGTTLDGLGRPMDQTKQLNGVPFKVNRSAPDPFRRNRIEQVI
                     PTGIKAIDGLLTIGEGQRVGIFAGSGVGKSTLLGMIARYCEADVIVIGLIGERGREVL
                     EFIEKDLGEEGYRKSVVVCATSDSPPLVRLKGAYVATAIAEYYRDQGKKVILMM"
     assembly_gap    647..656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <657..1198
                     /gene="fliI"
                     /locus_tag="PLT73_10165"
     CDS             <657..1198
                     /gene="fliI"
                     /locus_tag="PLT73_10165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721168.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HRM20773.1"
                     /translation="VTRVAMAQREIGLATGEPPTSKGYTPSVFTVLPKLLERSGMSEH
                     GSITAFYTVLVEGDDMNEPIADAVRGILDGHIILSRKIAAENQYPAIDVQNSVSRLMK
                     DIVPESHSRAAGMLKENMAIYAEAKDLIDVGAYRKGSSPEIDRAISLAPVIKNFLKQR
                     VDEPHSHNEIVDTLQNLFK"
BASE COUNT          353 a          258 c          356 g          289 t
ORIGIN      
        1 ttattggtga agtttgtgag attcagatca agtcgaccgg gaccactgtc ttgagtgaag
       61 tggtcggctt cgtggacgca accgtattac tgatgccact tgatgaaatc gatggaatag
      121 gaccgggctg tttggtgcgc ccaaccggaa agacacttca gattgaagtt agtgatgagc
      181 tgttgggaac cactctggat ggtttgggaa gaccgatgga tcaaacgaaa caactgaatg
      241 gtgttccgtt caaagtgaac agaagcgcac cagatccatt caggcgcaac agaatagagc
      301 aggtcatacc taccggaatc aaagcgatcg acgggctgct tacaatcggc gaaggacagc
      361 gggttggtat ctttgcaggt agtggtgtcg ggaaaagtac cctgttaggg atgatcgcac
      421 ggtactgcga ggccgacgtc attgttatcg gcctcatcgg agagcgtgga cgtgaagtgt
      481 tggaattcat cgaaaaggat ttgggcgaag aaggctaccg gaaatcggtg gtcgtctgcg
      541 ctacttcaga ttctccgccg ttagtacggc ttaagggtgc gtatgttgcc accgcgatag
      601 cagagtatta cagggatcaa gggaaaaaag tgattttgat gatggannnn nnnnnnccgt
      661 gacgcgtgtc gccatggcac aaagggaaat cggattggct acaggggaac caccgacatc
      721 gaaagggtat acaccttccg tgttcaccgt gcttccaaaa ttattggaga gaagcggcat
      781 gtccgagcat ggctcgatta ctgcctttta cacagtattg gttgaagggg atgacatgaa
      841 cgagcccatc gcagatgctg tccgagggat tttggacggc cacatcatcc tttcaaggaa
      901 aattgccgca gagaatcagt atccggcaat cgatgtccaa aacagcgtga gccgtttgat
      961 gaaagatatc gttccggaat cacacagccg tgcagccgga atgctgaaag agaatatggc
     1021 catctatgca gaagcgaaag acttgattga cgtaggtgcc tatcggaaag ggagcagccc
     1081 cgaaattgat cgggctattt cgcttgcacc cgtcatcaaa aacttcttga agcagcgtgt
     1141 ggatgaacca cattcacata atgaaattgt ggatacctta caaaatttgt tcaaatgatc
     1201 agaaatcaag gtgattagat gagaagacat cagttctcaa tggaaaaaat tctggagtgg
     1261 cgaatc
//
LOCUS       DAOMUJ010000538         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28822_length_1260_cov_1.944398, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000538 DAOMUJ010000000
VERSION     DAOMUJ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28822_length_1260_cov_1.944398"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLT73_10170"
     CDS             <1..668
                     /locus_tag="PLT73_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20774.1"
                     /translation="TKGSDGELVLERSFSNTHLTRIDGTSVYETDRKSYPRFVADAGI
                     RGFVIPIKEDYHDVLYPDLRDARQPDMFRDTSHGAGPKRPGNTIRKVYLCRAPSNLGA
                     PGSVLFFYKGKSIDSPSQALTAVGVLDDVSLATSTKDLMKLTGGRSVYSEKELEDYQA
                     TADDPVKVINYLLVDYIDPPISLDELQNTGIFAGHPPQSIVELKGRRLREALGRCNFG
                     FPL"
     gene            665..1234
                     /locus_tag="PLT73_10175"
     CDS             665..1234
                     /locus_tag="PLT73_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20775.1"
                     /translation="MTRRVGIVVGISGVGKTWLLEKLGAAIPMQILSASTLIRDSLTK
                     TEEQLTSQDHLRARNIDDNQAALLEAFNRHIDPDKTLVILDAHVVIDTPHGLVHIGSD
                     LFREMDPDFMVFVKDEPRKIHRNRERDVSRTRPERSVDSLAKHQEIAIAAARDISDNL
                     RIPIHFVNGGDDDALGRILRAVQEAEPDV"
BASE COUNT          311 a          334 c          324 g          291 t
ORIGIN      
        1 gaaccaaggg ttcagatggc gaacttgttc tcgaacgctc gttctcaaat acacacctga
       61 cccgtatcga tggtacctcg gtctacgaga cggaccgaaa aagctatccg cgtttcgttg
      121 cggatgcggg gatacgtggt ttcgttatcc caatcaaaga agactaccat gacgttcttt
      181 atccagacct ccgcgatgcg cgtcagccgg acatgtttcg cgacaccagt catggcgccg
      241 gtccgaaacg gccaggtaac accattcgaa aggtttacct ctgccgcgca ccgtcaaacc
      301 ttggcgcgcc gggatcagtc ctgttctttt acaaaggcaa atcgatcgac agcccgtccc
      361 aagcgctgac ggcggttggt gttcttgacg atgtttcact cgcgacgtca accaaagatc
      421 ttatgaagct gactggcgga aggtctgttt acagcgaaaa ggaactggaa gactatcaag
      481 ctacggccga tgatccagtg aaagtcatca attacctgct cgtcgattat attgatccgc
      541 caatctcgct ggacgagttg caaaatacag ggatttttgc cggacacccg ccacaatcca
      601 tcgtcgagtt aaagggcagg cgacttcgtg aagccttggg tcgctgcaac tttgggttcc
      661 cgctttgacg cgtagagttg gcattgttgt cggcatttcc ggagttggga agacgtggct
      721 actcgaaaaa ttaggggcgg ccatcccgat gcaaattctc tctgcaagta ctctgatccg
      781 agactcactc acaaagacgg aggaacaact gacgtctcag gaccatcttc gcgctcgcaa
      841 tattgacgat aaccaggcgg cgcttctcga agcgttcaac cgacacattg atcctgataa
      901 gaccctcgtc attttggatg ctcatgttgt aatcgatacc cctcatgggt tggtacatat
      961 cggctcagac ttattccgcg agatggatcc ggacttcatg gtctttgtca aagatgaacc
     1021 gcgaaagatc caccgaaacc gtgaacgcga cgtgagccgg acgcgtcccg agcggagcgt
     1081 cgattcgcta gccaaacacc aagagattgc aattgctgcc gcgcgcgaca tctcagataa
     1141 cttgagaata cctattcatt ttgtaaacgg tggcgatgac gatgcgcttg gccgaatttt
     1201 aagagccgtg caagaggcag aacccgatgt ctgacaacaa atcgattgca ttctggtgcg
//
LOCUS       DAOMUJ010000539         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_28848_length_1259_cov_1.897841, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000539 DAOMUJ010000000
VERSION     DAOMUJ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28848_length_1259_cov_1.897841"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLT73_10180"
     CDS             <1..1047
                     /locus_tag="PLT73_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20776.1"
                     /translation="FIINFPNTVMVVSHDRHFLNTVCTHMCDVDFGKIKLYVGNYDFW
                     MQSSQLAAKLAADSNAKKEEQIKELQDFIARFSANASKSKQATSRKKTLDKITLDDIQ
                     PSSRKYPFVGFTPEREIGNDLLMVSNISKTIDGVKILDNISFALNKNDKVAFTSRKDI
                     ATSTLFKILMGEMEADGGTFKWGVTTSRSYLPKDHTNEFPVADITILEWLRQFAGKEE
                     DDNTFLRSFLGRMLFSGEDVMKKVSVLSGGEKVRIILSKMMLSKANVLVLDDPTNHLD
                     LESITALNDGMIAFKGALLFTSHDYEFINTTANRIIELTPNGVVDRMETTYEDFLADK
                     DVQARINALYKEQA"
BASE COUNT          384 a          287 c          261 g          327 t
ORIGIN      
        1 ttcattatta acttccctaa cactgttatg gttgtttccc atgaccgtca cttcctgaat
       61 acggtttgta cgcacatgtg tgacgttgac ttcggaaaaa tcaaattgta tgtcggaaac
      121 tacgacttct ggatgcaatc cagtcaattg gctgctaaat tggccgctga ttccaatgcg
      181 aaaaaagaag aacaaatcaa agagttgcag gacttcattg cgcgcttcag tgcaaatgca
      241 tcgaaatcca aacaagcgac ctcccgtaaa aagacattgg ataaaatcac tttggatgat
      301 attcaaccat catcccgtaa atatcctttc gtcggcttca ctccggaacg cgaaatcgga
      361 aatgacttgt tgatggtttc aaacatctcc aaaacaatcg atggcgtcaa aattttggac
      421 aacatcagct ttgctttgaa caaaaacgat aaagtcgctt tcacaagccg caaagacatc
      481 gctacttcta ccctcttcaa gattttgatg ggcgaaatgg aagctgatgg cggtactttc
      541 aaatggggcg tcacaacttc aagaagctat ttgccgaagg atcacacgaa cgaattcccg
      601 gttgccgata tcaccatttt ggaatggttg cgccagtttg caggcaaaga agaggacgac
      661 aacactttct tgcgcagctt cttgggccgc atgctgttct caggtgaaga tgtcatgaag
      721 aaagtttctg tcctatccgg gggagaaaaa gtacgtatca ttctatcgaa aatgatgctt
      781 tcaaaagcaa acgttttggt gctggatgac ccgacaaatc acttggatct tgaatccatc
      841 acggcgttga atgacggcat gatcgctttc aaaggcgcat tgttgttcac ttcacacgat
      901 tacgagttca tcaacactac tgcgaaccgt atcatcgaat tgactccaaa cggcgttgtt
      961 gacagaatgg aaacaaccta cgaagacttc ttggcagata aagacgtgca agcaagaatc
     1021 aacgcacttt ataaagaaca agcataaaac cagcatgaaa aagaggaaca acccaagcgt
     1081 tcgggttgtt cctctttttt aataacatat ttcaattcca aaagtgcatc acctctggtg
     1141 tttcacaagg ttgcaaaata ttccatgcat cccaaaacgg tggctagctt cacgggtaag
     1201 cccttcggaa attagataaa tctgacccat tgcgctctac gatgctcatt cagggccag
//
LOCUS       DAOMUJ010000540         1247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29218_length_1247_cov_1.603188, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000540 DAOMUJ010000000
VERSION     DAOMUJ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29218_length_1247_cov_1.603188"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLT73_10185"
     CDS             complement(<1..148)
                     /locus_tag="PLT73_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013350.2"
                     /note="with PdxST is involved in the biosynthesis of
                     pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of
                     glutamine to glutamate and ammonia; PdxS utilizes the
                     ammonia to synthesize pyridoxal 5'-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRM20777.1"
                     /translation="MTKKIGVLALQGAVTEHLQALNKLGITASAVKLPADLDGLDRLI
                     IPGGE"
     gene            complement(151..1041)
                     /gene="pdxS"
                     /locus_tag="PLT73_10190"
     CDS             complement(151..1041)
                     /gene="pdxS"
                     /locus_tag="PLT73_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRM20778.1"
                     /translation="MTTKIIGSEKVKRGMAQMQKGGVIMDVVNAEQARIAEAAGAVAV
                     MALERVPSDIRAAGGVARMADPTIVEEVMNAVSIPVMAKARIGHITEARILEAMGVDY
                     IDESEVLTPADEEFHLLKSDYTVPFVCGCRDLGEALRRIGEGASMLRTKGEPGTGNIV
                     EAVRHMRKVNGQIRQLITKSDDELMMFAKEIGAPYELVKEIKLLGKLPVVNFAAGGVA
                     TPADASLMMSLGADGVFVGSGIFKAENPEKFARAIVRATTNYEDYALLAELSKGLGEP
                     MKGIDISTLHASERMQERGW"
BASE COUNT          271 a          342 c          323 g          311 t
ORIGIN      
        1 attcaccgcc agggataatc agcctatcca agccgtccag atcggcgggc agcttgactg
       61 cagatgcggt gatacccaat ttattcagag cttgcaggtg ctctgtgacg gcaccctgta
      121 aggccaatac gccgattttt tttgtcatgc ttaccagcct ctttcctgca tgcgttcgga
      181 agcgtggagc gtactgatgt cgatcccttt catcggttcg cccaagcctt tggacagttc
      241 ggccaacaag gcatagtcct cgtaattggt tgtcgcgcgg acgatggcgc gggcgaattt
      301 ttcgggattt tcggctttga agatgccgga tccgacgaag acgccatctg cgcccaaact
      361 catcatcaag gatgcatcag caggtgttgc gacgccaccg gcagcgaagt tcactacagg
      421 aagtttcccg agtagtttga tttccttgac cagttcgtaa ggagcaccga tttccttggc
      481 gaacatcatc agttcatcgt ccgattttgt gatcaactgg cggatttggc cgttgacttt
      541 gcgcatatgg cgtacggctt ccacgatatt tccggtccct ggctcgcctt tcgtacgcag
      601 catcgaagcc ccttcaccaa tccggcgcag cgcttcaccc agatcgcggc aaccgcagac
      661 gaaaggaaca gtatagtcgg atttcaaaag gtggaattct tcatcggcgg gggtcagcac
      721 ttcgctctcg tcgatgtagt cgacgcccat cgcttccagg atgcgggctt cggtgatgtg
      781 gccgatgcgg gctttcgcca tgacgggaat gctgacggcg ttcatcactt cctcgacgat
      841 ggtgggatcc gccatccgtg caaccccacc ggctgcgcgg atatccgagg gcacgcgctc
      901 caaagccatg accgccacgg caccagccgc ttcggcgatc cgtgcctgct ccgcgttcac
      961 gacatccatg atgacgccgc ccttttgcat ttgggccatc ccgcgtttta ctttttctga
     1021 accgatgatt tttgttgtca taaatgatcc tccttggttt aaatgataaa atgatgaaag
     1081 aatgcctaaa tcataatccc tttttccgga aacacctaca tccaattgga ctgttttcga
     1141 accaaccaat ttggtgtata atgattaaaa accaaggaga gtgaagcaat ggtggagtgg
     1201 agactggatc aggatagggg catgccgctc tatcaacagc tgatgca
//
LOCUS       DAOMUJ010000541         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29248_length_1246_cov_1.560873, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000541 DAOMUJ010000000
VERSION     DAOMUJ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29248_length_1246_cov_1.560873"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLT73_10195"
     CDS             complement(<1..269)
                     /locus_tag="PLT73_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRM20779.1"
                     /translation="MENPIYADIVNKIQAYASIIIHRHLRPDPDALGSQNGLASLIRQ
                     AYPEKKVFVAGENEDSLSYLGSMDSIPDEAYEGALVIVTDTANQP"
     gene            complement(281..>1246)
                     /locus_tag="PLT73_10200"
     CDS             complement(281..>1246)
                     /locus_tag="PLT73_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRM20780.1"
                     /translation="IVGNRNDIQEFALEKGAAVLITGGFDTDESIVHLADQVEMPILR
                     TTYDTFTVATMINRAMTDQLIKKEIMLVEDIYTPIDETKFLSLDDTVFQYRNLNTEST
                     HSRFPIVNHNMRLVGIITAKDILGKADTLSLERVMTRDPIVAKTHMSVASVAHRMIWD
                     GLEVMPVVKDNLQLLGIISRQDVMKAMQLAQRQPQVGNTIEDQVVENLSLISSEEGTA
                     LPSYNFRITPQMTNSLGTVSFGVLSEVLASTTKKILYTLQKGNALIEQMDIYHLKMIQ
                     IDSVIEIRSKMLELGRRSSKLDVEVYLENSLVGKAIVICQLMEKS"
BASE COUNT          298 a          323 c          270 g          355 t
ORIGIN      
        1 cttggttgat tcgcggtatc cgtgacgatc accaaggcac cctcgtaggc ttcatccggg
       61 atgctgtcca tactgccgag atacgagaga ctatcttcgt tttcgccggc aacaaatact
      121 tttttctcag gataggcttg cctgatcaaa ctggcgagac cattctgcga acccaacgca
      181 tccggatccg gacgcagatg acgatggatg atgatactgg catacgcttg aatcttgttg
      241 actatatctg cataaatcgg gttttccatt cgcttccctc ctaggatttt tccatcagtt
      301 ggcaaataac gattgctttg ccgaccagag aattttctaa ataaacttcg acatccagtt
      361 tggaggatct tctgcccaac tccaacattt tcgaacgaat ctcaatgaca ctgtctatct
      421 gtatcatctt caaatgatag atgtccatct gctcgattaa tgcattccct ttctgtaagg
      481 tatacaagat tttcttggtc gtgctcgcca acacttcact caataccccg aacgacaccg
      541 ttcccaggct attcgtcatt tgtggtgtga ttctgaaatt ataggaaggc aaagcagtcc
      601 cttcttcaga gctgatcaaa gacaagtttt caacgacttg gtcctcaatc gtattgccta
      661 cttgcggctg acgctgtgca agctgcatgg ctttcataac atcctggcgg gaaatgatac
      721 cgagcagctg caagttatct ttcaccacag gcatcacctc taagccatcc cagatcatcc
      781 ggtgagccac gctcgctaca ctcatgtgcg tttttgcgac tatcggatcg cgggtcatta
      841 cccgttcaag cgaaagcgta tctgcttttc caaggatatc cttcgcagtt atgatgccca
      901 caagtcgcat gttgtgattg acgatcggga accgtgaatg ggtgctttct gtattgaggt
      961 tgcgatactg gaaaacagta tcatccaatg aaagaaattt cgtctcatca atcggcgtgt
     1021 agatgtcctc caccagcata atttcttttt tgatcagctg gtccgtcatg gcgcggttga
     1081 tcatcgtcgc tacggtaaaa gtgtcatagg tcgtacgcag tatcggcatt tcgacctgat
     1141 ccgccaaatg gacgatgctc tcatctgtat cgaacccccc cgtgataagg acggctgcgc
     1201 ccttttccaa cgcgaattcc tggatatcat tccggttacc gacgat
//
LOCUS       DAOMUJ010000542         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29418_length_1240_cov_1.624473, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000542 DAOMUJ010000000
VERSION     DAOMUJ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29418_length_1240_cov_1.624473"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLT73_10205"
     CDS             <1..252
                     /locus_tag="PLT73_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM20781.1"
                     /translation="TLAIVLGVLAITVVGGIGPIKQLVAENCGIAFLYRISVEKELEE
                     GTLKEIPIRRFAVAHPFHVVHLKDFRDRGPLQELLSFYR"
     gene            479..>1240
                     /gene="guaC"
                     /locus_tag="PLT73_10210"
     CDS             479..>1240
                     /gene="guaC"
                     /locus_tag="PLT73_10210"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0015950 - purine nucleotide interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HRM20782.1"
                     /translation="MKIFDYEDVQLIPNKSVVQSRSECDTTVVLGGRTFKLPVVPANM
                     QTILDEELAEKLAREGYFYIMHRFDEASRLPFIQHMHEKGLFASISLGIKAAEFDFVN
                     ELAAKKAVPEYTTIDVAHGHSDEVIRMIKHVKEVMPETFLIAGNVGTPEGVRELENAG
                     ADATKVGIGPGKVCTTKIKTGFGTGGWQLAAVSWCAKAASKPIIADGGVRTNGDIAKS
                     IRFGATMVMVGSLLAGHDESPGETKEINGGLYKEYF"
BASE COUNT          322 a          267 c          342 g          309 t
ORIGIN      
        1 accctcgcca tcgtcctcgg cgtcctcgcg atcaccgtcg tcggcggcat cggcccgatc
       61 aagcagttgg tggcggagaa ctgcggcatt gcttttctct accgcatatc ggtcgaaaag
      121 gaactggagg aggggacgct gaaggagatt ccgatccggc gcttcgctgt ggcgcatccg
      181 ttccatgttg tccatctgaa ggattttcgt gatcgcgggc cgctccagga actgttgtct
      241 ttttatcgat gatggggatg cacggaactt tgctgggatg cggcattcct cggattttcg
      301 tgacgcggat aagaaatccg aacgtttcat caccaattaa tctttgacaa acgtggggca
      361 gtagtgtaac ttactatagg gactttacga tagaagacat tttttgcctt cacgtccgtg
      421 tctgtgtaaa gcgattccca aaaaggattt gtgcactagc agagagaggg aaatagaaat
      481 gaaaattttt gattatgaag acgtacaatt aattccgaat aagagcgtgg tgcagagccg
      541 ttctgaatgc gatacgactg tcgttttggg tggccgtacg ttcaaattgc cggtggtgcc
      601 tgcgaatatg cagacgatcc tggacgaaga actcgctgaa aagttagcca gagaaggtta
      661 tttttacatt atgcatcgtt ttgatgaggc gagccgtttg ccgttcatcc aacacatgca
      721 tgaaaaaggt ttgtttgctt ccatcagttt ggggatcaaa gcagcagaat tcgatttcgt
      781 aaatgaattg gcggctaaaa aagccgttcc tgaatataca acaatcgacg tggcgcatgg
      841 tcattccgat gaagtcatcc gtatgatcaa acacgtcaaa gaagttatgc cggaaacatt
      901 cctgattgcc ggtaacgtgg gcactcctga aggcgtccgt gaattggaaa acgctggagc
      961 tgatgctaca aaagtaggaa tcggacctgg taaagtctgt acaaccaaaa tcaagactgg
     1021 tttcggtaca ggcggctggc aattggcggc ggtaagctgg tgtgcgaaag ctgcaagcaa
     1081 accgatcatc gctgatggcg gcgtgcgtac aaacggagat atcgctaaat cgatccgctt
     1141 tggtgcaaca atggtcatgg tcggctcatt attggctgga cacgatgagt caccgggtga
     1201 aacgaaggaa atcaacggcg gcttgtacaa agagtacttc
//
LOCUS       DAOMUJ010000543         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29438_length_1239_cov_1.887669, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000543 DAOMUJ010000000
VERSION     DAOMUJ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29438_length_1239_cov_1.887669"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="ilvA"
                     /locus_tag="PLT73_10215"
     CDS             complement(<1..982)
                     /gene="ilvA"
                     /locus_tag="PLT73_10215"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HRM20783.1"
                     /translation="MTDELVNKEDVLKAYKVLRNVVKQTPLQHDAYLSQKYHANIFLK
                     REDLQIVRSFKLRGAYYAISQLSEAETENGVICASAGNHAQGVAYTCNHLGIKATIFM
                     PSTTPSQKIDQVRYFGKDYVEIKLIGDTFDESNKAAHSYAEEHKLTFIEPFNDRNVIA
                     GQGSLAVEIHQDLVAEGETADMVFAAIGGGGLISGISSYMKEAMPETKIIGVESLGAP
                     GMKASLDADKVTTLTDIDKFCDGTAVATVGDLTFRHCQKNVDDILVVPEGQVCGTIID
                     LYTKQAIVAEPSGALSVSALEQYKEEIRDKTVVCIVSGGNNDINRMAEIDE"
     gene            complement(1066..>1239)
                     /locus_tag="PLT73_10220"
     CDS             complement(1066..>1239)
                     /locus_tag="PLT73_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRM20784.1"
                     /translation="KDVLTDIQTGAFAKRFTDDYKAGFPDFHAMRAEQQGHQIEAVGA
                     ELRKMMPFVNEQQ"
BASE COUNT          297 a          299 c          273 g          370 t
ORIGIN      
        1 gctcatcgat ttcagccatt cggttgatat cattatttcc gccgctgacg atgcagacga
       61 ccgttttatc tctgatttct tctttatatt gttccaaggc agaaacggat agtgcgccgg
      121 atggttccgc tacaatcgct tgtttagtgt ataaatcgat gatggttccg caaacttgtc
      181 cttcaggaac gaccagaatg tcatcgacat ttttttggca gtgtctgaaa gtcaagtcac
      241 cgacagtcgc tacggctgtc ccatcacaga atttatcaat atcagtcaaa gtcgtgactt
      301 tatccgcatc aagcgaagct ttcatacctg gggcgcccaa ggactccaca ccgatgattt
      361 ttgtttctgg catggcttcc ttcatgtagc tgctgatgcc tgaaatcaaa ccgcctccgc
      421 cgattgcagc gaataccata tcagccgttt ctccctctgc cacgagatcc tggtgaattt
      481 ccactgccaa actgccttgc ccggcaatca cattgcgatc attgaaaggt tcaatgaaag
      541 tcagcttgtg ttcttctgca tagctatggg cagctttatt cgactcgtcg aaagtatctc
      601 cgatcagttt gatttcaaca taatcctttc cgaaataacg gacttggtca attttttggg
      661 aaggggttgt cgatggcatg aagatggtcg ctttgatacc cagatggttg caggtatagg
      721 ctactccttg ggcatgattg ccggcggagg cacagattac tccgttctcc gtttcggctt
      781 cgctcagctg cgaaatcgca taataagcac ccctcaattt gaaggatcgg acgatctgta
      841 ggtcctcccg tttcaaaaag atattggcat gatacttctg cgatagatac gcatcatgct
      901 gcaaaggggt ctgcttgacc acgttgcgca gtactttata tgctttaagc acgtcttctt
      961 tgttcactaa ctcatccgtc attctatctg ctcctaatct attgaataca atgatgaaga
     1021 gagaaggctt aaaaataagg atgctctcaa cctacatctg attcattatt gttgttcatt
     1081 cacgaaaggc atcattttac gcaattcagc accgactgct tcgatctggt ggccttgttg
     1141 ctcagcgcgc attgcgtgga agtcagggaa gccagctttg taatcgtcag tgaagcgttt
     1201 tgcgaacgcg ccagtttgaa tgtcagtcaa aacgtcttt
//
LOCUS       DAOMUJ010000544         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29466_length_1238_cov_1.875740, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000544 DAOMUJ010000000
VERSION     DAOMUJ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29466_length_1238_cov_1.875740"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLT73_10225"
     CDS             complement(<1..508)
                     /locus_tag="PLT73_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20785.1"
                     /translation="MIRFDHVNVIREKRKILNDINWHAKKGEHWAILGLNGSGKTTLL
                     QLLNGYLWPSSGKLVVLGETFGQTAIPELRKRIGWVSSALQQQLNPNDIAEHIVLSGK
                     FASIGVWTIPTEAEKQQALELLIKCGGKELIGFRYGILSQGQQQIILIARALMAEPEI
                     LILDEPCNG"
     gene            683..>1238
                     /locus_tag="PLT73_10230"
     CDS             683..>1238
                     /locus_tag="PLT73_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRM20786.1"
                     /translation="MEQNKEKVSLIIEGGAMRGVFCAGIISTLLRQEIYLDYVIGVSS
                     GSANGINYVSRDLTRTKASFVDIAANPAFSGMKYLLNGQGYFNTKYIYEDAITTDIPF
                     QFETFKQNPTSMKIVVTDMETAKPVYFDKDDIATSAELGLKIRASSTVPLLMPPTQIN
                     GKYYLDGGIVDSLPIEKAIADGNHK"
BASE COUNT          315 a          316 c          288 g          319 t
ORIGIN      
        1 ggccattgca gggctcgtcc aaaatcagta tttccggttc ggccatcaaa gcgcgcgcta
       61 tcaaaatgat ctgctgttgc ccttgggaca ggatgccgta gcgaaacccg atcaattctt
      121 tgccgccgca tttgatcaag agctccagcg cctgttgttt ttcagcttca gtcgggatgg
      181 tccagacgcc tatcgaagcg aatttcccgc tcaagacgat gtgttcggcg atgtcgttgg
      241 gattcagctg ttgctgcagg gccgagctga cccagcctat ccgtttgcgc aactcgggga
      301 tggccgtctg cccgaaagtc tcgcccaaca cgaccagttt gccgctgctg ggccacagat
      361 agccgttcaa gagttgcagc aaggttgtct tgccggagcc gttcaagccg agtatggccc
      421 aatgctcgcc ttttttggcg tgccaattga tgtcgttcaa aattttccgt ttttcgcgaa
      481 tgacgttcac gtggtcgaat ctgatcattc catcacccca tctatagttt tgtggtcatt
      541 aggtatcttt attgcgaata taccacattt gccccatccg gaaaaggctt tcgtcatcgt
      601 gtgccctgcg caacaaaata atgagaaaag catcgcatca caccgcaatg tcatctatac
      661 tatagagtga ggtggaaaat gtatggaaca gaataaagaa aaagtatcct taatcataga
      721 aggaggcgcc atgcggggcg tcttttgcgc aggaatcatc agcactttgc tgcgtcaaga
      781 aatctacttg gattatgtca tcggtgtctc ttccggatcg gccaatggca tcaattatgt
      841 cagccgggac ctgacccgca cgaaagcttc atttgttgat atcgccgcca atccggcctt
      901 ttcgggaatg aaatatttgc tcaacggaca aggttatttc aataccaaat atatttatga
      961 ggatgcaatc acgaccgata tcccctttca attcgagaca ttcaagcaaa atccaacttc
     1021 catgaaaatc gttgtcacgg atatggaaac cgcaaaacct gtttatttcg ataaggatga
     1081 catcgcaacc agtgcagaat tgggtctcaa aatcagggca tcatccactg tacctttgct
     1141 gatgccccca acccaaatca atggcaaata ctatctggac ggcggtatcg tcgactcgct
     1201 tccgattgaa aaagcgatcg ctgacggcaa ccataagc
//
LOCUS       DAOMUJ010000545         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29471_length_1238_cov_1.532544, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000545 DAOMUJ010000000
VERSION     DAOMUJ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29471_length_1238_cov_1.532544"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLT73_10235"
     CDS             complement(<1..477)
                     /locus_tag="PLT73_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20787.1"
                     /translation="MDKKEFETEMIHQYKLNGYNIVMDTYSGAVHVVDDLAFDIIALY
                     EATPIEEIVTMMLEQYANDPEINEQEIRDTLSDVEELKANGELFTEDMYQNLSVDLKN
                     RQTFVKALCLNVAHTCNLTCDYCFASQGKYNGDRAIMKFEVGKQSISCLRIPDSTVT"
     gene            complement(519..674)
                     /gene="scfA"
                     /locus_tag="PLT73_10240"
     CDS             complement(519..674)
                     /gene="scfA"
                     /locus_tag="PLT73_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRM20788.1"
                     /translation="MKRIVTLSTRNLKDTAKHGGCGACQTSCQSACKTSCGIANQSCV
                     NPKQAAK"
     gene            complement(845..>1238)
                     /locus_tag="PLT73_10245"
     CDS             complement(845..>1238)
                     /locus_tag="PLT73_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20789.1"
                     /translation="MTSVCDSDVAMDALADALLAIDGTLDSKSGEHTLSNGTLLFSLP
                     DKQCEIWQALESEKVDCPIENAIGQMAAEYVWAYPPGIPLLVPGERISNGFIRQVHTL
                     LEKGTKVYSDADGLPKNIQIMHHESLAH"
BASE COUNT          290 a          299 c          283 g          366 t
ORIGIN      
        1 agttacggtg gaatccggaa ttctcaagca ggaaatcgat tgcttgccga cttcgaattt
       61 catgatcgcc cggtcgccgt tgtacttgcc ttggctcgcg aaacagtaat cgcaggtcag
      121 gttacacgtg tgcgccacgt tcaggcacag agccttcacg aaggtttggc ggttcttcaa
      181 gtcgacggaa aggttttggt acatgtcttc cgtgaacaat tctccgttgg cctttaattc
      241 ttcaacatcc gacagcgtat cacggatttc ttgttcgttg atttcagggt cgttcgcata
      301 ttgttctaac atcatcgtga cgatttcttc gattggcgtt gcttcataga gtgcgatgat
      361 gtcgaaggca agatcatcca cgacatgcac agcaccgctg taggtatcca ttacgatatt
      421 atatccattc agtttatatt gatgaatcat ttcggtttca aactcctttt tgtccataaa
      481 attgccgcct aactgaaatc agtcaggcgg actctttttt acttcgctgc ttgtttaggg
      541 ttcacacagc tctgattagc gattccgcaa gaagttttgc atgcggattg gcaagatgtt
      601 tggcatgcgc cgcagccgcc gtgtttggca gtatccttca ggttgcgggt gcttaatgtt
      661 acgattcgtt tcataacgac atctcctctg aaataggtta aggaagcata ttgcttgaca
      721 gaaaaacgct ccctttgtga ttaattttac caacaaatta ttagcccgtc aatctaccgt
      781 cctatcagca tagtgaccgt ttttatcgct atcgtgattc atccaataac tatcaaaatg
      841 caagttagtg agcaaggctt tcgtgatgca ttatttggat atttttcgga agcccatcag
      901 catcgctgta gacctttgtc cccttttcca aaagcgtatg gacttggcgg ataaacccat
      961 tgctgatccg ttccccaggc accagcaaag gaattccggg cggataagcc cagacgtatt
     1021 cggctgccat ttgccctatc gcgttttcaa tcgggcaatc tactttttcc gattccaagg
     1081 cctgccagat ttcgcattgc ttatcgggta aggagaatag caacgttccg ttggacagag
     1141 tgtgctcgcc agacttagag tcgagcgtgc cgtcgatggc caatagcgca tcggccaaag
     1201 catccatagc gacgtcacta tcgcagacgc tggtcatc
//
LOCUS       DAOMUJ010000546         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29477_length_1238_cov_1.354184, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000546 DAOMUJ010000000
VERSION     DAOMUJ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29477_length_1238_cov_1.354184"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLT73_10250"
     CDS             <1..251
                     /locus_tag="PLT73_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20790.1"
                     /translation="YLLLPLDILPRQRGFGGLSRAVLGHFAPPKGFWRVISAQRGSIR
                     IQKKLHHNQKSRSGYDAAPIYVAVSNGLLGAQQTSSSV"
     gene            complement(267..>998)
                     /locus_tag="PLT73_10255"
     CDS             complement(267..>998)
                     /locus_tag="PLT73_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM20791.1"
                     /translation="KDTDGDGTIDQYGSYGFKWQDAVNGYGISLFDEEGASSNLNQSD
                     VREALSYTQKLKDLNKGYQVSSEDFDKGNVAFCPLTFAQYRTYQPYPYRVSKYSTFKW
                     SCLAMPGTIANQKTSQIATSLIAINSKTTQQQLAFEFLETLTIDQEIQQELFENSQGA
                     SALKSVMASDSTKALLDQDAMSNYSLKQDVLDDIIENAVIEPKFKKYNNIIERCDYLI
                     TNALNENKIEDELVDIEKEIDNLLK"
     assembly_gap    999..1008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1009..>1238)
                     /locus_tag="PLT73_10260"
     CDS             complement(<1009..>1238)
                     /locus_tag="PLT73_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM20792.1"
                     /translation="LAEDFNKLSSLGALMPLDELIKANNVSTDIFYESALGSGSYQNT
                     QYALPYEINPTMMCVNRDLLEKEGIAIPEGNW"
BASE COUNT          311 a          287 c          243 g          387 t
ORIGIN      
        1 gctatctctt gttgcccttg gacattttgc cccgccaaag gggttttggc gggttatctc
       61 gtgctgtcct tggacatttt gccccgccaa aagggttttg gcgggttatc tctgcgcaga
      121 gaggatcaat ccgcatacaa aaaaagctgc atcataacca gaaatcccgt tctggttatg
      181 atgcagcccc tatttatgtt gcagtctcca acggattact cggcgctcaa cagacctcat
      241 cctccgtgtg acatttgtca ttccgatcat tttaaaagat tatcgatttc tttttcgatg
      301 tcgaccagct catcctcaat tttattttca ttcaaagcat tcgtgatcag ataatcgcat
      361 ctttcgatga tattgttgta ctttttgaat ttcggttcga tcaccgcatt ttcgatgata
      421 tcatccagta catcttgttt taaggaataa ttgctcatgg catcctgatc cagcagcgct
      481 ttggtgctgt cgctggccat cacactcttc aaagccgaag ccccttgcga attttcaaat
      541 agttcctgtt gaatttcttg atcaatcgtt aaggtttcaa gaaactcaaa agctaattgt
      601 tgctgtgtcg ttttggaatt tatggctatc aaagaggtgg ctatttggga agtcttttga
      661 ttagcgattg tcccgggcat cgccaagcaa ctccatttga aagtagaata tttactgacg
      721 cgataaggat aaggttgata cgtccggtat tgcgcgaatg tcaacggaca aaaagcgaca
      781 ttgcctttat cgaagtcttc cgaagatact tgatagcctt tattcagatc ttttagtttt
      841 tgagtatagg ataaagcttc ccttacatcg ctttgattca gattgcttga ggcaccctcc
      901 tcatcaaaca agctgatgcc ataaccattg accgcatctt gccatttaaa accataggag
      961 ccatactgat cgatcgtgcc atcaccatcc gtatccttnn nnnnnnnncc aattaccctc
     1021 aggtatcgcg ataccttcct tttccaacag atcgcgattg acgcacatca tggtcgggtt
     1081 gatctcatag ggtaaggcat attgcgtatt ttggtaactg cccgaaccga gcgcactttc
     1141 atagaagata tcagtactta cgttgttagc tttgatcaat tcatccaacg gcatcaatgc
     1201 gcccaatgaa gacaacttat tgaaatcttc agccaata
//
LOCUS       DAOMUJ010000547         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29555_length_1235_cov_2.279661, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000547 DAOMUJ010000000
VERSION     DAOMUJ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29555_length_1235_cov_2.279661"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLT73_10265"
     CDS             complement(<1..595)
                     /locus_tag="PLT73_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRM20793.1"
                     /translation="MKSELCELLGIQYPIIQGAMAWVADADLASAVSNAGGLGLIGTG
                     HDSVDVANEKISKMKALTDKPFGVNIMLLNPHVDAIVEAVIAAGVKVVTTGAGSPARY
                     MNQFKEAGITVIPVVASVALARRMEKDGAHAVVVEGMESGGHIGKTTTLALVPQVVDA
                     VSIPVIAAGGIGEASTYYLQSNARGVLSAFDEIFSRAS"
     gene            complement(621..848)
                     /locus_tag="PLT73_10270"
     CDS             complement(621..848)
                     /locus_tag="PLT73_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM20794.1"
                     /translation="MTFEKIQAIIVDQLDKEVEEVQLATNFREDLDADSLDLFQIIND
                     IEDEFDIKIESDEGINTVEDLVKYVDAQLAE"
     gene            complement(875..>1235)
                     /locus_tag="PLT73_10275"
     CDS             complement(875..>1235)
                     /locus_tag="PLT73_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRM20795.1"
                     /translation="QAFLTMDGRAIFDFAIRSVPSVIREVMEKGNLSDGDLKKILAHQ
                     ANVRLLEAISKKVKLPFSLFATNIAETGNTSAASIPILLDQLVQTKEIQLGTKDKVLM
                     TGFGGGLTWGAIVVSLN"
BASE COUNT          320 a          308 c          239 g          368 t
ORIGIN      
        1 tagacgcacg gctgaatatt tcatcaaaag cagaaagcac gccacgtgca ttgctctgca
       61 aataatacgt actggcttca ccaataccgc ctgcagcgat gacaggaatg gaaacggcat
      121 cgacaacctg aggaaccagg gcaagtgtcg ttgttttccc gatgtgacca ccggattcca
      181 tgccttcgac tacgacagca tgtgcgccat ctttctccat tcttctcgct aaagctacgg
      241 aagccactac cggaataacg gtaatgcctg cttctttgaa ttggttcata taacgggctg
      301 gacttccggc gccggttgtg acaaccttaa cgccggcagc gataactgct tctacgatag
      361 catcgacatg cggattcaaa agcatgatgt ttacaccgaa aggcttatcc gtcaatgctt
      421 tcattttcga aattttctca ttcgctacat ccacactatc atgtccagtc ccgataagac
      481 ccaaaccacc agcattggaa acggcacttg ccaaatcagc atccgctacc cacgccattg
      541 ccccttgaat gatgggatat tgaataccta ataattcaca taattctgat ttcatacaag
      601 gcctactttc ctgctactgc ttattctgct aattgagcgt caacgtattt aactaaatct
      661 tcaacagtgt tgatgccttc gtctgattcg attttgatgt cgaattcgtc ttcgatgtcg
      721 ttgatgattt ggaataagtc taagctgtcc gcatctaagt cttctctgaa gttagttgct
      781 aattgtactt cttctacttc tttgtccaat tggtccacga taatagcttg aattttttcg
      841 aatgtcataa taaattcctc caaatatgtt ttttttagtt taatgatact acgatggctc
      901 cccaagtgag gccgccacca aatcctgtca tcagcacctt atctttggtg ccgagttgaa
      961 tttccttggt ttgaacaagc tgatccaaca atatcggaat gcttgctgct gaagtgttgc
     1021 ccgtttccgc aatgtttgtg gcaaacagtg aaaatggcaa tttcactttt ttgctgattg
     1081 cctccaataa gcgcacattt gcttgatgag ccaaaatctt cttcagatcg ccatcagaaa
     1141 gatttccctt ctccatcact tcgcgtatga cgcttggcac actgcggatt gcaaaatcaa
     1201 agatggctct gccatccatc gtaaggaaag cttgt
//
LOCUS       DAOMUJ010000548         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29564_length_1235_cov_1.976271, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000548 DAOMUJ010000000
VERSION     DAOMUJ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29564_length_1235_cov_1.976271"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..761)
                     /locus_tag="PLT73_10280"
     CDS             complement(228..761)
                     /locus_tag="PLT73_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20796.1"
                     /translation="MEMTRQKLMQLLDELIEECHTRGIEGTIAIYGGTAMGYYVPELR
                     TTQDVDSMFRPFSEIQSAAEVVGNRNNVPNNWLNMQIHDVMPPIGDDNPEVYSDANGL
                     KVTFASKKYLLAMKAMSDRRSQKDLDDAAVLFNALRLKNWMGIDEIVHQYYGVESAGA
                     QELFFEDIEDRADEIRK"
     gene            complement(752..>1235)
                     /locus_tag="PLT73_10285"
     CDS             complement(752..>1235)
                     /locus_tag="PLT73_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20797.1"
                     /translation="NMAESVVSRTLSGKTVPTFNKVDAAARTLGYELTLSPLERVTVR
                     VIGPAMSDLMQSIQQYSGTEEQWTRVSAGMRNLMEFPDDEPADFKKVTKGMDVRWQAF
                     MAGLYDYQHWSKGDVSPQSLKLDAEWTPLRKIYRSATPPDEFFAWYNVIIPRGELLWK
                     "
BASE COUNT          332 a          355 c          252 g          296 t
ORIGIN      
        1 gaacagttgg aggagcgtac cgaagctcgc aaggccaagg atttcgcccg cgccgacgcc
       61 attcgtgatg ccttgggcgc agccggaatc gccatcgaag acaccccaca aggccccacc
      121 tggaccctca aataatcccc catctcttct gaaaaaaacg ttggccggtc taccccagac
      181 cggccaattt catatcagaa acctaaaagt cattcctaaa tctatcttta cttccgaatc
      241 tcatcggcac gatcttcgat atcttcaaaa aagagttcct gagcacccgc agattccacg
      301 ccgtagtatt gatgaacaat ctcatcaata cccatccaat ttttcagccg caaagcatta
      361 aacagcactg ccgcatcatc aaggtctttc tgcgatctac gatcagacat cgctttcatc
      421 gccaaaagat atttcttcga agcaaacgta actttcaaac cattagcatc gctatacact
      481 tccggattgt catcccctat gggcggcata acatcatgga tttgcatatt aagccagtta
      541 tttggcacat tattacgatt cccgacaact tccgccgcgc tttgaatttc agaaaaagga
      601 cggaacatgg aatcgacatc ttgtgtagtt cgcaattccg gaacgtagta gcccatcgcg
      661 gtcccgccat aaatggcgat agttccttcg attccgcgag tatgacactc ttcaatcagc
      721 tcatcaagaa gctgcatcag tttttgtctg gtcatttcca tagcagctca cctcgcggaa
      781 tgattacgtt gtaccacgca aagaactcat ccggaggcgt ggcggaccga taaatctttc
      841 gcaacggggt ccactcggca tcaagtttca gcgattgagg ggatacgtcc cctttactcc
      901 agtgctgata gtcatagagc ccagccataa aggcctgcca acgcacgtcc atccccttag
      961 tgactttctt aaaatcagca ggctcatcat caggaaattc catcagattg cgcattccgg
     1021 ctgaaacacg ggtccattgc tcttccgttc ccgaatactg ctgaatcgac tgcatgagat
     1081 cactcattgc agggccgatt acgcgaacag tcacacgctc caaaggggaa agcgtcagct
     1141 catatcccaa agtacgcgcc gcagcatcaa ccttattgaa ggtgggaacg gttttgccac
     1201 tcaacgttct cgaaacaacg gattcagcca tgttc
//
LOCUS       DAOMUJ010000549         1234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29602_length_1234_cov_1.782867, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000549 DAOMUJ010000000
VERSION     DAOMUJ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29602_length_1234_cov_1.782867"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLT73_10290"
     CDS             complement(<1..204)
                     /locus_tag="PLT73_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341308.1"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HRM20798.1"
                     /translation="MVRICFIGAGSTIFAKNVLGDAMLTPSLQDAEIALYDIDENRLK
                     ESELMLQTINKNSNQNRAKIKSFS"
     gene            complement(301..1134)
                     /locus_tag="PLT73_10295"
     CDS             complement(301..1134)
                     /locus_tag="PLT73_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRM20799.1"
                     /translation="MSEKLLKDLFFKEYYFSDFYFFNVGYEKCQSKHRFGPSLRDNYI
                     VHFVISGKGWYTVNDTTHYLGTGDFFLIRPNELVDYEADVQDPWEYYWIGFSGTKVKE
                     ILHTNGIGAKDYIGQVGAQKELQEKFAHFMESDFFDDAKKLANQALFYDIFSFFKIHN
                     ENMEMGVRISRTKKYSEAFLLYVGNNYYREDLTIEEIAKSMYLHPAYFSQVIKEELGL
                     TALKYLNLYRMNKASQLLKTTELSVEQIASAVGYQNRHSFTRAFKNRFQSSPTRYKLE
                     K"
BASE COUNT          319 a          272 c          242 g          401 t
ORIGIN      
        1 gctgaaagat tttatttttg cgcggttttg attcgagttt ttattgatgg tctgaagcat
       61 cagttcggat tctttcaagc ggttttcatc aatatcgtac aatgcaattt ctgcatcctg
      121 gagactcggc gtcaacatcg catcccctaa aacatttttt gcgaaaattg tgctgcctgc
      181 accgataaaa caaattctga ccatgttcat gctccttttc cttttgatgt cttcagtata
      241 ggctattctc actgaataag aagggcatgg ttttagcttc agggtacaat ttttcaggtc
      301 ttatttttcg agcttatacc gggtcgggga actttggaat ctgtttttga aggcccgtgt
      361 aaaagagtgc cggttctggt atcctaccgc gcttgctatt tgctccaccg acaattccgt
      421 tgttttcaac aactggctag ctttgttcat gcggtataga ttcaaatatt tgagggcggt
      481 caagcctaac tcctccttga ttacctggct gaaataggct gggtggaggt acatcgattt
      541 cgcaatttct tcgattgtca gatcctcgcg ataataatta tttcccacat acaacaaaaa
      601 agcttcactg tatttttttg ttcggctgat gcggaccccc atttccatat tttcattatg
      661 gattttgaag aatgaaaata tgtcatagaa gagcgcctga ttcgccagtt ttttggcatc
      721 atcaaaaaaa tcggattcca tgaaatgcgc gaatttctcc tgcagctctt tttgtgcgcc
      781 aacttgaccg atatagtctt tggctccgat cccgttcgta tgcaggattt ctttcacttt
      841 ggtgcccgaa aatccgatcc aatagtattc ccatggatct tgcacatccg cttcgtaatc
      901 gaccagttca ttcggacgga tcagaaaaaa atcaccagta cccaagtaat gcgttgtatc
      961 gttcacggta taccaccctt ttccactgat cacaaaatgc acgatgtagt tgtcccgtaa
     1021 cgaaggccca aaacgatgct tgctctggca cttctcgtag cccacattaa aaaaataaaa
     1081 atccgagaaa tagtattctt taaaaaatag atccttcaat agtttttcgc tcatcgggta
     1141 tcctctgtta tctgtttatt tatcggtata tttgtatagg tagattttag cacaacacct
     1201 gctaacggtg acagaaaagg atcagataca ccgg
//
LOCUS       DAOMUJ010000550         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29630_length_1233_cov_1.939728, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000550 DAOMUJ010000000
VERSION     DAOMUJ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29630_length_1233_cov_1.939728"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>532)
                     /locus_tag="PLT73_10300"
     CDS             complement(<1..>532)
                     /locus_tag="PLT73_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRM20800.1"
                     /translation="TTSAYVNIQQIVRDTVTEIGYTRGKFGFDADNLAVMVSLDEQSD
                     DIAIGVNESLERRELVTTDYNEIGAGDQGLMFGFAINETEELMPLPISLSHKLAKRLS
                     EVRKDGTLEYLRPDGKTQVTVEYDEDGKPKRVDTIVLSTQHDENVTLEQLKKDILEHV
                     IHVVIDDALLDADTKY"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<543..713)
                     /locus_tag="PLT73_10305"
     CDS             complement(<543..713)
                     /locus_tag="PLT73_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016647535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRM20801.1"
                     /translation="MVEKRLFTSESVTEGHPDKIADQISDAILDTLLAADPNARVACE
                     TIVTTGLVVVFGE"
     regulatory      complement(711..786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1021..>1233
                     /locus_tag="PLT73_10310"
     CDS             1021..>1233
                     /locus_tag="PLT73_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20802.1"
                     /translation="MLTIQKQFMNLMHNTFLSMLTAPLTLLLFGAWRGTTFNGPNYLL
                     LFILYLFLLFSHALERLLSKREHSKRK"
BASE COUNT          338 a          287 c          219 g          379 t
ORIGIN      
        1 aagtatttcg tatcagcatc caataaggca tcgtcgatga ccacatggat gacatgctcc
       61 aaaatatctt ttttcaattg ttccaacgtg acattctcat catgttgcgt gctcaaaacg
      121 attgtgtcca cacgtttcgg tttgccgtct tcgtcgtatt ccacggtgac ttgtgttttt
      181 ccgtccggtc ttaaatattc caaagtaccg tctttgcgga cttcagataa acgtttcgct
      241 aatttatgac taagcgaaat aggtaatggc atcagttctt ccgtttcgtt gatggcaaaa
      301 ccgaacatca acccttgatc ccctgccccg atttcattgt agtccgtagt caccagttct
      361 ctgcgctcca gtgattcgtt gacaccgatt gcgatatcgt cggattgttc gtccaaggat
      421 accataacgg ccaaattatc cgcatcgaaa ccaaatttac cccgcgtata accaatttca
      481 gttacagtat cgcggacgat ctgctggata tttacgtatg cgcttgtcgt gannnnnnnn
      541 nnttctccga agacaaccac cagtccggtc gtgactatcg tttcgcaagc aacacgcgcg
      601 tttggatcag cagccaacaa cgtatccaaa attgcatcgc tgatctgatc agcgatttta
      661 tccggatgac cttccgttac tgattctgaa gtaaataatc ttttttctac catttttcat
      721 tcccccattt tttggttaca aggcgtcttc acatattgtg aatcctatcg ggatattgca
      781 attagcctct gaaatgcaac atgtctaata tacgctatca tcgctaaaaa agcaacataa
      841 aataagaaga tttcatcttt gtatcataga tgacgcaaac aaatactgat gtaaatattt
      901 tacgtcagct tttttcaatc ctgacatcgc ttgacatcgc aaagacgtta acctatgatg
      961 aatttgcgca atagatacga gatgaaacac actctatcat taatagagaa ggaagagatt
     1021 atgctgacaa tccagaaaca attcatgaat cttatgcaca ataccttttt gtcgatgctg
     1081 acagccccgt tgacgctgct tcttttcggg gcatggagag gaacgacttt caacggtccc
     1141 aactatctgt tactatttat actttatctt tttttgcttt tttcccatgc cttggaacgc
     1201 ctactttcaa aaagagaaca ttccaaaaga aag
//
LOCUS       DAOMUJ010000551         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_29869_length_1225_cov_1.789744, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000551 DAOMUJ010000000
VERSION     DAOMUJ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29869_length_1225_cov_1.789744"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..244)
                     /locus_tag="PLT73_10315"
     CDS             complement(44..244)
                     /locus_tag="PLT73_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20803.1"
                     /translation="MTMKKFQVELPEDMLEKMRKGYAAKRKQGGYEVKNISDEELAEY
                     FMSMGLNYVDRKISEILDKENK"
     gene            complement(631..840)
                     /locus_tag="PLT73_10320"
     CDS             complement(631..840)
                     /locus_tag="PLT73_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20804.1"
                     /translation="MKFGMRKPSLKRSLKARTTGKMKRAFKRKTNPFYGKQGMGLIRD
                     AKRTAYNRIYKKATFSIWDLFKKRK"
     gene            complement(894..>1225)
                     /locus_tag="PLT73_10325"
     CDS             complement(894..>1225)
                     /locus_tag="PLT73_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HRM20805.1"
                     /translation="GAEATIVGAFDTVAYEVSYDPTNGDPRVENHQWVIQEEIMDAGT
                     DPFGVGSEVMLGAEHMEGMNGATATIDAAEMTTVYMVDYTDTVTGETIKNHKWVIEQE
                     LAPVEAP"
BASE COUNT          291 a          297 c          238 g          399 t
ORIGIN      
        1 agaaaaagag aagcaatcac tgctggattg cttctctcat agattattta ttttctttat
       61 cgagaatttc ggatattttt ctgtcgacat aatttaagcc catgctcatg aaatattcag
      121 ccagctcttc atcgctgata tttttcactt cgtagccgcc ctgttttctt tttgcggcat
      181 agccttttct cattttttcc agcatatctt cgggtagctc cacttgaaat ttcttcattg
      241 tcatcgcaac cgcctcctat gtcattgtca gcctgaacga tcggataaga tactggttat
      301 attccgtatt gatcaggtaa cgctttcatt atacccttct tttcaaaata tatacagaat
      361 tttgtgcata aaacaggatt atatatattt acagataggc ggattactcc tatatcggca
      421 acatgctgct gaagcacacc accagtccga tagaaagcat cagcgaacag atcggctaca
      481 ggaatgtcac tttcttttat caaaaattca aacaagccta cgctatgacg ccgcacgaat
      541 acaggatgaa cgtgaattga gcgagcattt ttgcactaaa caaaaaatga ccgctttccg
      601 gttaaggaaa cgatcatttt tattgttggt ttattttctt tttttgaaca gatcccaaat
      661 cgagaacgtg gcttttttat atattcggtt gtaggccgtc cttttggcgt cgcggatcag
      721 gcccattcct tgtttgccat agaacggatt ggttttccgt ttgaacgcac gcttcatctt
      781 cccggttgtc ctagccttca atgagcgttt caggctcggt ttgcgcatac cgaatttcat
      841 tacgaccctt cctttcttag aggcaggaat atttctggat gttcgaatgt tagtcacggc
      901 gcttcaactg gtgcaagttc ctgttcgatc acccacttgt gatttttgat cgtttcccca
      961 gtgaccgtat ctgtataatc caccatatag actgtggtca tttcagcggc atcaatagtc
     1021 gcagtggcgc cgttcatgcc ttccatgtgc tcagctccca acatcacttc gctgccgacg
     1081 ccgaatggat ccgttccggc gtccatgatt tcttcctgga tcacccattg atggttctcc
     1141 actctcgggt ctccattggt tggatcatag gaaacttcat aggcaaccgt gtcgaaggct
     1201 ccaacgattg tcgcttccgc acctt
//
LOCUS       DAOMUJ010000552         1219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30077_length_1219_cov_1.632302, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000552 DAOMUJ010000000
VERSION     DAOMUJ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30077_length_1219_cov_1.632302"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>824)
                     /locus_tag="PLT73_10330"
     CDS             complement(<1..>824)
                     /locus_tag="PLT73_10330"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM20806.1"
                     /translation="VATGQGEKQIVCGAPNVAGGQKVIVALHGARITGNAKIKKGKMR
                     GQESNGMICSLAELGYSESVVSKKYADGIFVLPAEAVPGTEVVDLLELDDAILDIDIT
                     PNRADALSMRGSAYEVAAIYNKALKFPEAPVSEKTGSVTEYIKVAVEDTNDTPAYHIQ
                     VIKDVKIAESPLWLQNKLMNGGIRPINNVVDITNYILLEYGQPLHAFDYDQIGSKEII
                     VRRAKENETMTTLDGVERTLDTDNIVITNGTAPIALAGVMGGLDSEITDGTVTVAL"
     assembly_gap    825..834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<835..1044)
                     /locus_tag="PLT73_10335"
     CDS             complement(<835..1044)
                     /locus_tag="PLT73_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20807.1"
                     /translation="MKVSYKWLKEYLDLSDVTPDELAEKMSRTGIEVDDVIYPGKGLS
                     KIVVGETLSVVDYPDSDHLHVCQVNI"
     gene            complement(1049..>1219)
                     /gene="pheS"
                     /locus_tag="PLT73_10340"
     CDS             complement(1049..>1219)
                     /gene="pheS"
                     /locus_tag="PLT73_10340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710243.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM20808.1"
                     /translation="VHPNVLEMSGIDSTKYSGFAFGLGQERVAMLKYGVDDIRHFYQN
                     DIRFLSQFDVKE"
BASE COUNT          295 a          313 c          258 g          343 t
ORIGIN      
        1 ccaaagcgac agtcacggtt ccatcagtaa tttctgaatc cagaccgccc atgacaccag
       61 ccaatgcaat cggtgcagta ccattcgtga tgacgatgtt atctgtgtcg agcgttcttt
      121 cgaccccatc aagcgtcgtc attgtttcat tctctttggc acgacggaca atgatttcct
      181 tggaaccgat ttggtcataa tcgaaagcat gcaatggctg gccgtattcc aacaggatat
      241 aattcgtgat gtcgactacg ttgttgatcg gacggatacc gccgttcatc agtttattct
      301 gcagccaaag cggactttcc gcaattttta cgtctttgat cacttggata tggtaggcag
      361 gagtatcgtt cgtatcctca acggcaactt tgatgtactc cgtaacagaa cctgtttttt
      421 cagaaaccgg agcctccggg aatttcaatg ctttgttgta gatggctgca acttcatagg
      481 cggatccgcg catgcttaaa gcatcggcac ggttaggcgt gatatcgatg tccaggattg
      541 catcatccag ttccaacaag tcaacgactt ctgttccggg aacagcttca gcaggcagga
      601 caaatatccc gtccgcatat ttttttgaaa caacgctttc cgagtagccc agttcagcca
      661 atgaacagat catcccgttc gattcttggc cgcgcatctt acctttcttg atttttgcgt
      721 tgccggtgat ccgcgcgcca tgcaaagcca cgatgacttt ctggccgcca gccacattcg
      781 gcgccccgca gacgatctgc ttctcgccct gccccgtcgc caccnnnnnn nnnngatatt
      841 tacttggcag acatgcagat ggtctgaatc aggatagtcg actacggaca acgtttcccc
      901 gacgacgatc tttgacaaac ctttgcccgg atagatgacg tcatccactt caataccggt
      961 acgcgacatt ttttccgcta attcatctgg tgttacatct gatagatcta aatattcttt
     1021 taaccattta taggatactt tcattatgtt actcctttac atcgaattga cttaagaatc
     1081 ggatatcatt ttggtagaag tggcggatgt catcaacacc atatttcaac atcgctacac
     1141 gttcctggcc tagaccaaat gcaaatccgg agtatttcgt agagtcaatt ccagacattt
     1201 ccagaacatt gggatggac
//
LOCUS       DAOMUJ010000553         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30132_length_1217_cov_2.317556, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000553 DAOMUJ010000000
VERSION     DAOMUJ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30132_length_1217_cov_2.317556"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLT73_10345"
     CDS             complement(<1..360)
                     /locus_tag="PLT73_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054506.1"
                     /note="metalloprotease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRM20809.1"
                     /translation="MLHQQIEQNKRRTVLIVFLFFLLFAALGAAIGYLNWDNALSGLA
                     LALTIGVAYVAIMVAQSTQVVMSLNNAREITDKAQFPMLWNIVEELTIVTRLPFPRIF
                     IIEDESPNAFAAGNSPEK"
     gene            complement(397..954)
                     /locus_tag="PLT73_10350"
     CDS             complement(397..954)
                     /locus_tag="PLT73_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010054505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM20810.1"
                     /translation="MEWLIGIIVVAAIIGAVWVSLYNSLVKNRLWVTEAWSQIDVQLK
                     RRNDLIPNIVETVKGYAKYEQETLTKVINLRNLISDMGTEDPAKKMELSNQLSSQLRT
                     VFALAEAYPDLKANQNFLALQEELSATENKIAYARQLYNSSVTQYNIKLGTFPSNIVA
                     NVHGFRPEVVLATPEEEKNVPKVSF"
BASE COUNT          316 a          307 c          244 g          350 t
ORIGIN      
        1 cttttccggc gaatttccgg cagcaaatgc attcggactt tcatcctcga tgatgaagat
       61 tctcgggaac ggcaaccgtg tcacaatcgt cagttcttcc acaatattcc aaagcatcgg
      121 gaattgcgcc ttatcggtta tttcccgggc attgttcaag ctcatgacca cttgggtgga
      181 ttgtgccacc atgatggcga cgtaggccac tccgatagtc agcgcaagcg caagcccgga
      241 caaggcatta tcccaattca gatagccgat ggccgccccc agtgccgcaa acaggagaaa
      301 gaataagaag acaatcagta cggtgcgccg tttattctgc tcaatttgtt gatggagcaa
      361 gggtactcac caactttcca tcaaaatcat attttctcag aacgatactt tcggtacatt
      421 cttttcttct tccggagtag ccagaacgac ttccggacgg aaaccatgga catttgctac
      481 aatattgctc gggaatgtgc ctagtttgat gttgtattgt gtgacactgg aattgtacag
      541 ttggcgcgca taagcgattt tattttcagt agcggacaat tcttcctgta aagccaggaa
      601 attctggttc gccttcagat cagggtatgc ttccgccaaa gcaaatacgg tccgcaattg
      661 actcgaaagc tgattggaca attccatctt cttggccgga tcttccgttc ccatatccga
      721 aatcagattg cgcaaattga tgactttcgt caaagtttct tgctcatact tcgcataacc
      781 tttgactgtt tcaacgatgt tcgggatcag gtcgttcctg cgtttcagtt ggacatcgat
      841 ttgactccaa gcctctgtta cccataatct atttttcaca agactgttat acaacgaaac
      901 ccatactgcc ccgatgatcg ctgccactac aatgattcct attaaccatt ccattttgct
      961 cacaactccc tttctctcgc gccgcctctg cagctgacct agacaacttt ccgtctacac
     1021 cttccattat agaagagtct attcgttttg taaaaaaata gacgaacgat ttctgcgccg
     1081 aaaagttccg gcatctgtgc tatcatgaag gaaagaaaaa tagatgaggt gtacttatgt
     1141 attttatccc gcaacatact ttcaaaaatc tgcccatcct cttcaaaact gatcttttgt
     1201 acaaaggtgt cactgca
//
LOCUS       DAOMUJ010000554         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30271_length_1212_cov_2.552290, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000554 DAOMUJ010000000
VERSION     DAOMUJ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30271_length_1212_cov_2.552290"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..356
                     /locus_tag="PLT73_10355"
     CDS             75..356
                     /locus_tag="PLT73_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006588315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM20811.1"
                     /translation="MIKSFADKETEKVYNQLFSKKLPQSIQKIALRKLIMIDNAGCLE
                     DLRVPPANQLELLKGDRAGQYSIRINGQYRVCFNVDGNSFFNVEIVDYH"
     gene            396..692
                     /locus_tag="PLT73_10360"
     CDS             396..692
                     /locus_tag="PLT73_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015639060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRM20812.1"
                     /translation="MTGYIDTPTIGEILQEEFMEPMDISAYKLAQEIHVPVSRIQDIL
                     HDRRKITADTSLRLAKYFGVSDRYFLDMQNDIDIRNLKISLAEEINKIRVCQAV"
     gene            complement(843..1127)
                     /locus_tag="PLT73_10365"
     CDS             complement(843..1127)
                     /locus_tag="PLT73_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM20813.1"
                     /translation="MFTLKEFGNRISMLRKLQNLTQDELAERIHVSKDHISRIERGIR
                     ACSLESLIDLAQELHTSTDYLLTGTGPANDFAKEQLLSAIEVLSSVSKLI"
BASE COUNT          371 a          231 c          279 g          331 t
ORIGIN      
        1 ggaatgattt tccaattttg aacagaaaat tatacccgct tgacatatac atgaaaaacg
       61 ggtataatga aatcatgata aagagctttg cagacaagga aacggaaaaa gtttataatc
      121 agcttttttc aaagaagtta ccccagtcga ttcaaaaaat tgcattgcga aaattgatta
      181 tgattgataa tgcaggatgt ttggaggatc tgcgtgttcc gccagcaaat cagctggaat
      241 tattgaaagg tgatcgggcg ggacaataca gcatacgaat caatggtcag tatagggtct
      301 gcttcaatgt agatggaaac agttttttca atgtcgaaat tgtcgattat cattagtgca
      361 gactgcgcat aatatgcaag gagaggagaa tttcaatgac aggctatatt gatactccta
      421 caatcggaga aatcttacag gaagaattta tggaaccaat ggatatctcg gcatataagc
      481 tggctcagga aatccacgtt cctgtatcca gaattcagga tatcctgcat gaccgcagaa
      541 aaataactgc tgatacgtct ctcagactgg caaagtattt tggcgtatca gatcggtatt
      601 ttttggatat gcagaatgat attgatatcc gaaatctgaa gatcagcctt gccgaggaaa
      661 tcaacaaaat cagagtgtgc caagctgttt gacggcacac tcgcattgac agtgcaaggc
      721 ttatagtatc cttaaaccac aagaccttcc ggaatctgac cttacaatag gttgattccg
      781 ggaggtcttt atcgttgtga accctacgat aggctggacg atatgggaat aagcaccaat
      841 ttttatatca gcttacttac ggaagatagt acttcgattg ccgacaaaag ctgctccttt
      901 gcaaagtcat tggcggggcc ggttcctgtc agaagataat ctgtactggt gtgcagctct
      961 tgtgctagat caataagaga ttccaacgaa caagcacgga taccacgctc aatgcggctg
     1021 atatggtctt tgctgacatg aatccgttcg gctagttcgt cctgcgtaag attctgtaat
     1081 ttacggagca tactgattcg attgccaaat tccttcaagg tgaacataat gagtgtcctt
     1141 tccgccggat gcggaggttg aggacacttc actgtgggac agttgacggg cttttctcag
     1201 cgaaaaaaag ac
//
LOCUS       DAOMUJ010000555         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30280_length_1212_cov_1.740709, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000555 DAOMUJ010000000
VERSION     DAOMUJ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30280_length_1212_cov_1.740709"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLT73_10370"
     CDS             <1..808
                     /locus_tag="PLT73_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM20814.1"
                     /translation="LARVITEHNMQIDDRTEKTDMNLLLKIAMWSAPPLLLVILQNDL
                     GTTLVFLAIIIGMTFLSGVSWKILLPVFGIIGALGSLLIYLVVYNRDVLLNFGFQNYQ
                     FARIDSWLDPFGDSTGDAYQLAQSMKAIGSGKLFGKGLGVSEVHVPVRVSDMIFSTIG
                     ENFGFIGGCFLIFIYFLLIYQMIRICFDTKNEFYAYMATGVIMMILFHVLENIGMTIG
                     LLPITGIPLPFISQGGSALLGNMLGIGLVMSMRYHYRSYMFSEEDEHFGI"
     gene            833..1186
                     /locus_tag="PLT73_10375"
     CDS             833..1186
                     /locus_tag="PLT73_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HRM20815.1"
                     /translation="MITIYQHPQCSTCKKTRAWFDEEGIAYELKDIREERPDRESIRQ
                     AIASGNLTLRRMFNTSGNLYKEMQLKDKLDSMELEEALDLLESDGMLIRRPFVTDGTQ
                     ITVGHDEEKLSTTWK"
BASE COUNT          301 a          315 c          308 g          288 t
ORIGIN      
        1 gttggcgcgg gtcatcaccg agcacaatat gcagattgat gaccggaccg aaaaaaccga
       61 catgaacctg ctgctgaaga tcgccatgtg gtcggcgccg ccgctccttt tggtcatcct
      121 gcagaacgac ctcggtacaa cactcgtttt tctggcgatc atcatcggca tgacgttcct
      181 ttccggcgta tcctggaaaa tcctgttgcc cgtattcggg ataatcgggg cactcggatc
      241 gttgctcatc tacctggttg tctacaaccg cgatgtgctc ctgaatttcg gtttccagaa
      301 ttaccaattc gcccggatcg attcctggct cgacccgttt ggggacagta ccggggacgc
      361 ctatcaattg gcgcaaagca tgaaagcgat cggatcaggt aaactgttcg gcaaaggcct
      421 cggcgtttcc gaagtccacg tgccggtgcg cgtctccgat atgatctttt ccactatcgg
      481 cgagaatttc ggtttcatcg gcggttgttt tctcattttt atctattttc tgctgattta
      541 ccaaatgatc cgcatctgtt ttgacacgaa aaatgaattt tatgcctaca tggcgacagg
      601 cgtcatcatg atgatcctgt tccacgtatt ggaaaatatt ggcatgacga tcgggttgtt
      661 gccgatcacc ggtattccgt tgccgttcat ctcgcaaggt ggctcggcgc tactcggcaa
      721 tatgctgggg atcggactgg tcatgtcgat gcgctaccat taccgcagct acatgttctc
      781 ggaagaggac gaacatttcg gaatctgaat ctaaacccaa ggaggcactt atatgatcac
      841 aatttaccaa catccccaat gctcgacctg caaaaaaacg cgcgcttggt tcgacgaaga
      901 aggcatcgcc tacgaattga aggacatccg cgaagagcgt cccgatcgtg aaagcatccg
      961 ccaagcgatc gcatccggca atttgacgct tcgccgtatg ttcaacacga gcgggaatct
     1021 gtacaaggaa atgcaactga aggacaaact cgacagcatg gaacttgaag aagcgcttga
     1081 cctgctggaa tcggacggca tgctgatccg ccggccattc gtaacggacg gcacgcagat
     1141 cacggtcggc cacgacgaag aaaaattaag cacgacctgg aaataagcta aggagggaaa
     1201 aacatgactg aa
//
LOCUS       DAOMUJ010000556         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30297_length_1211_cov_2.353806, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000556 DAOMUJ010000000
VERSION     DAOMUJ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30297_length_1211_cov_2.353806"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLT73_10380"
     CDS             <1..314
                     /locus_tag="PLT73_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010013804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20816.1"
                     /translation="LFFAGQMTGVEGYVESAASGLLAGLNAARMASGQECVVFPKETM
                     IGAMSHYITNTDSKHFQPMNANFGIIEPLGGKKIRDKQLKNQMIADKALAALDEFVQT
                     I"
     gene            386..>1211
                     /locus_tag="PLT73_10385"
     CDS             386..>1211
                     /locus_tag="PLT73_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM20817.1"
                     /translation="MSNQELIDRFIKYLAIERRYSDETVKAYLFDLKKFESFLEESGT
                     SNLLAVKLYDVRLYLSFLDEQKLSRNTISRTLSSLRGFYHFLIRNDLLDENPLSYISF
                     KKKQLRLPQYLYEEELEKLLRAAEGTEMLDYRNRALVELFYATGIRVSECKNIKLQDV
                     SFELGVILIFGKGNKERYVPFGHYAAAAIQEYLEMTRTELMAKHQRNHDYLFVSRLGD
                     PLTAGGIEYILKQIMKKTGLTGTLHPHMLRHTFATDMLNNGADMRTVQELLGHASLS"
BASE COUNT          348 a          264 c          289 g          310 t
ORIGIN      
        1 acctgttctt cgccggccag atgaccggag ttgaaggata tgtcgagagt gctgcaagcg
       61 ggctgttggc tggcctcaat gccgccagaa tggcgagcgg acaggaatgt gttgtcttcc
      121 cgaaagagac gatgatcggt gcgatgtccc actacatcac aaatacagac agcaaacatt
      181 tccaaccgat gaacgccaac ttcggcatca ttgaaccttt aggcggcaag aaaatccgcg
      241 ataaacagct caaaaaccag atgatagccg ataaagcatt ggctgctctg gacgaattcg
      301 tgcaaacgat ttaagttcgc caacttacat tgaaatctct ccaacattgt gataaattta
      361 gtcataagat gttggaggga tttttttgtc taatcaagaa ttaatcgatc gtttcataaa
      421 atatttggct atcgagaggc gctattccga cgaaaccgtc aaggcctatc tgtttgacct
      481 gaaaaaattt gaatcctttc ttgaggaaag tggaaccagc aatttgctgg cggtgaaatt
      541 gtatgatgtc cggctgtatt tgagtttttt ggacgaacaa aaactgagtc ggaatacgat
      601 ttcgcgcaca ttgtccagcc tgaggggatt ctatcacttc ctgatccgca atgaccttct
      661 tgatgaaaac cctttatcct atatctcatt caagaaaaag caattgcgtt tgccgcagta
      721 cttatacgaa gaagagttgg aaaaactatt gcgcgcagcc gaagggacgg aaatgctcga
      781 ctatcggaac cgagcgctgg tggaattgtt ttatgcgacc ggaatccgtg tcagcgagtg
      841 caagaacatc aagttacagg atgtttcctt cgaattgggt gtcattttaa ttttcggaaa
      901 aggaaacaaa gaacgttatg tgcccttcgg ccattatgcc gccgctgcca ttcaggaata
      961 tctggaaatg acccggacag aattgatggc taaacaccaa cgcaatcacg attatctctt
     1021 tgtgagccgc ttgggtgatc cgctcacggc aggcggtatt gaatacatat taaaacagat
     1081 catgaaaaaa accggattga ccggtacctt gcatccgcat atgctgcggc atacgtttgc
     1141 gaccgatatg ttgaacaacg gagcagatat gcggacagtc caggaacttt taggacatgc
     1201 cagcctgtct t
//
LOCUS       DAOMUJ010000557         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30454_length_1207_cov_1.353299, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000557 DAOMUJ010000000
VERSION     DAOMUJ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30454_length_1207_cov_1.353299"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>746)
                     /locus_tag="PLT73_10390"
     CDS             complement(<1..>746)
                     /locus_tag="PLT73_10390"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRM20818.1"
                     /translation="GAVALFTVDLTAEPIGNLEWLLAPGESAKCKHADVSDKKHAADK
                     HEGTADCCQGEGEDTLYAAIINEQMAGVQHAYAAYQQDPIEPESAHALRVAIRQLRAL
                     LNFNKANGEEKYYREAGEDWREIATTFGYLRELDVLMEECRELRGLYPDMLAEDGQVM
                     TWLLEERQGEQDSIHELITSGALTERILDAEAATSHFLKSINLDDDQQKKATIKKIEK
                     MKKRLMRKWEEVDFSNHEQTHSLRINAKKL"
     assembly_gap    747..756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<757..1140)
                     /locus_tag="PLT73_10395"
     CDS             complement(<757..1140)
                     /locus_tag="PLT73_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM20819.1"
                     /translation="MGGQLILIRHGIAEERTPEGDDFYRKLTEAGVEELTAFLPDIAP
                     LLTESGNLKIWTSPLVRARQTAELVAEATSVEVIQPQAFLATGDYIAFMEALKQEDDG
                     FTLALVGHEPYMSSWTKELIGAAIPF"
BASE COUNT          226 a          356 c          278 g          337 t
ORIGIN      
        1 cagtttcttg gcgttgatgc gcaggctgtg ggtctgttca tggttggaga aatcgacttc
       61 ttcccacttg cgcatcagtc gtttcttcat cttttcaatt ttcttgattg tcgctttttt
      121 ctgctggtca tcatccaagt tgattgattt caggaaatgg gaagttgcgg cttctgcatc
      181 caggatgcgt tccgtcaatg cgccacttgt gatcaattca tggatgctgt cctgttcgcc
      241 ttgacgctct tccagcaacc aagtcatgac ctggccgtcc tcggccagca tatccggata
      301 caaaccgcgc agttcgcggc attcttccat cagcacatcc agctcgcgca ggtatccgaa
      361 ggtagtggcg atttcccgcc agtcttcgcc cgcttcccgg taatatttct cttcgccgtt
      421 cgctttgttg aaattcaaca aagcccgcag ttgacggatc gcgacgcgta gcgcatgcgc
      481 cgattccggc tcgatcggat cctgttggta agccgcatag gcatgctgaa cgcctgccat
      541 ctgttcgttg atgatagcag cgtacaaagt gtcttcgccc tcgccctgac agcaatccgc
      601 ggtcccttca tgcttatccg ccgcatgttt tttatcggag acgtctgcgt gtttgcattt
      661 cgctgattcc cccggcgcca aaagccactc cagatttcct atcggttccg ctgtcaaatc
      721 gaccgtgaac aatgcaaccg ccccttnnnn nnnnnngaac ggaatcgcgg caccaatcaa
      781 ttctttcgtc caactgctca tatacggttc gtggccgaca agcgccaagg tgaagccatc
      841 gtcctcctgt ttcaatgctt ccatgaaggc aatgtaatcg ccggtcgcca aaaacgcctg
      901 cggttggatc acttcgacgg aagtggcttc agccaccaat tcagcggttt gacgcgcccg
      961 aaccaatggg cttgtccata tcttcaggtt tccgctctcg gtcagtaagg gtgcgatgtc
     1021 gggcagaaac gcagttagtt cctccactcc tgcttccgtc agttttctgt agaagtcatc
     1081 cccttcaggc gtacgctctt ccgcgattcc atgccgaatc agtatcagtt gtcctcccat
     1141 actgctcatc ctcctttgtt tcggtgctcc aaatacggat atcacgtccc gtttttgtaa
     1201 ccgctct
//
LOCUS       DAOMUJ010000558         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30564_length_1203_cov_1.824913, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000558 DAOMUJ010000000
VERSION     DAOMUJ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30564_length_1203_cov_1.824913"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLT73_10400"
     CDS             <1..191
                     /locus_tag="PLT73_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM20820.1"
                     /translation="SFSENLAIGSEPVHNSDAYLLFNKETDQAFIDAVNTALEELKAD
                     GTIKELSEEYLDGDYVPE"
     gene            207..917
                     /locus_tag="PLT73_10405"
     CDS             207..917
                     /locus_tag="PLT73_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM20821.1"
                     /translation="MSFKFEVMIQSLQVASQYIGTTLFMAITAIVIGVILGLGIALIR
                     LYRIKGLSQFFQVVVTLLKGIPMVLIILALYLIASKQFDVWSESMNWSLRFRDFNMVW
                     IAISALSLLATINSSEIFRGAFAAIKKGQFDAAKSMGMTNLQTIRRVLLPQAIPISLP
                     MFSNLLISLIKASSLASMVSVVDVFAAAKITAQQNYRFLEAYVAVAVIYWGISLVIEK
                     GAQMLEQRMTRKLRRAIL"
     gene            914..>1203
                     /locus_tag="PLT73_10410"
     CDS             914..>1203
                     /locus_tag="PLT73_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM20822.1"
                     /translation="MMEVRNIHKRFGKQEVLKGIDMDVAKGEVVTLLGPSGSGKTTFL
                     RCLNFLERADEGSILLGETETIVNKANPKEILAHQRQTAMVFQNYALFHNKTA"
BASE COUNT          326 a          286 c          299 g          292 t
ORIGIN      
        1 aatccttcag cgaaaactta gcgatcggca gcgaaccagt ccacaactca gatgcatacc
       61 tgctgttcaa caaagaaacc gaccaagcct tcatcgatgc cgtgaataca gcattggagg
      121 aattaaaagc ggatggaacg atcaaagaac tttccgaaga atatctagat ggtgattacg
      181 ttccagaata agaaaggatg agggccatga gtttcaaatt tgaggtgatg atccaatcat
      241 tgcaagtagc aagccaatac attggcacaa cattattcat ggccatcaca gcgatcgtca
      301 tcggcgtcat tttagggtta gggatagcgc tgatccggct gtatcgcatc aaaggactga
      361 gccagttttt ccaagtggtt gtcactttgt tgaaaggcat cccgatggta ttgatcattc
      421 tcgcgcttta cctgatcgct tccaaacagt ttgatgtttg gtcggaatcc atgaactggt
      481 cgctgcggtt cagggatttc aacatggttt ggatcgccat ttccgcactg agtctgttgg
      541 ccacgatcaa cagcagcgag attttccgcg gtgccttcgc ggctatcaaa aaaggccagt
      601 tcgatgccgc caaatcgatg ggcatgacca acctgcagac aatccgccgg gtgctgcttc
      661 cccaggcaat cccgatttca ttaccgatgt tcagcaattt attgatcagc ttgattaagg
      721 cttcatcctt agcctcgatg gtcagtgtcg tcgatgtctt tgcagccgcg aagatcacag
      781 cccagcaaaa ctaccggttc ctggaagcct atgtggcggt tgcggtcatc tactggggca
      841 tcagtctagt gatcgagaaa ggtgcgcaaa tgcttgaaca gcgcatgacc cgtaaattga
      901 ggagggcgat tttatgatgg aagtacgcaa tatccacaaa cgcttcggca agcaggaagt
      961 cctcaaaggc atcgatatgg atgtcgccaa aggggaagtt gtaacccttc tggggccaag
     1021 cggttccggc aaaacgactt ttctccgctg tctgaatttt ttggaacggg cagatgaagg
     1081 cagcattctc ctgggagaga ccgaaacgat cgttaacaaa gccaatccaa aagagatttt
     1141 ggcgcatcaa cgccaaactg cgatggtctt tcaaaattat gcgctgttcc acaacaagac
     1201 agc
//
LOCUS       DAOMUJ010000559         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30673_length_1200_cov_1.186026, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000559 DAOMUJ010000000
VERSION     DAOMUJ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30673_length_1200_cov_1.186026"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..388
                     /locus_tag="PLT73_10415"
     CDS             131..388
                     /locus_tag="PLT73_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRM20823.1"
                     /translation="MSSYRIDELSVDQLDRISLPKFQRGFVWTKKKQEDFVQTLHDGY
                     PFGTLLVYPENDNDKDAKLQLLDGQQRLSTIGKIHCSFGNR"
     gene            385..1011
                     /locus_tag="PLT73_10420"
     CDS             385..1011
                     /locus_tag="PLT73_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20824.1"
                     /translation="MNRESYTSVYQSVKKMLPEGDKFTEPIFDKLVNSNPNKVAYWAM
                     DSLSSKEDVKAAMQSIDDLKQQIRSFVNLEHLKVPMIVYLGGSAHIADVFANLNKGGV
                     PLTKYEVFGAAWVNAAIRLRGAEESPLQDQLLQYVKNYYLDMRKQAEFDVDDFSEDEL
                     TQNRTVTLPEFGTALGQYVVDHLSALVPETTSAAPEIGSWSGHELGQS"
BASE COUNT          329 a          244 c          296 g          331 t
ORIGIN      
        1 atgacccacc ccacctatgg cgacggcgga atggccgccg ccgttcggct acaaagctga
       61 gtctgactgt taatacgctt gatctatcaa ctgtttggtg agatcattgg cgtgacgtaa
      121 ggagccgctg atgtcctcat accgtattga cgaattatcc gttgaccaac tcgatcgcat
      181 aagtttgcca aaattccaac gagggtttgt ttggacaaaa aagaagcagg aagatttcgt
      241 gcagaccctg catgatggat acccctttgg tactttactt gtttatccgg agaacgataa
      301 cgataaagat gcgaagctcc aactattgga cgggcagcag cgtctatcta ctatcggcaa
      361 gatccattgc agttttggaa accgttgaac agggaatcgt atacaagcgt ctatcagtct
      421 gtaaagaaga tgttgccgga aggggataag ttcacagagc cgatatttga taagctggtg
      481 aatagcaatc ccaataaagt ggcatattgg gctatggatt cgttgagcag caaagaggat
      541 gtaaaagctg ctatgcaaag cattgacgat cttaagcaac aaatccgctc attcgtcaat
      601 cttgagcacc ttaaagtgcc gatgattgtt tatttgggtg gatctgctca tattgctgac
      661 gtttttgcta acctcaataa aggtggtgtc cctctgacca agtatgaagt gtttggcgct
      721 gcttgggtta atgccgccat tcggctacga ggggctgagg aatctccgtt gcaagatcag
      781 ctgttgcagt atgtgaaaaa ttactatctt gatatgcgca agcaagctga atttgatgta
      841 gatgattttt ctgaagatga actgacccag aatcgcacgg tcacgttgcc ggaatttggt
      901 acagcgttgg gccagtatgt tgtagatcat ttgtcggctc ttgtccctga gacaaccagt
      961 gccgctccag agattggctc ttggagtggc catgaacttg gacaatcgta agttgagttc
     1021 actcaataag tatattcaga aaattagaga cgaactggaa gatattctcc agaagaccga
     1081 acgcatatgc aataatttgc agagtatgtt tgagacctta ttgcgtcgat ttaaatctac
     1141 tggcaatgat tatgaaaacg gcctatcttc gacgtttaag acgttatctt atttcgcggc
//
LOCUS       DAOMUJ010000560         1195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_30842_length_1195_cov_1.854386, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000560 DAOMUJ010000000
VERSION     DAOMUJ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30842_length_1195_cov_1.854386"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLT73_10425"
     CDS             <1..277
                     /locus_tag="PLT73_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRM20825.1"
                     /translation="MDADSLLLDVNVHPSKKEIRISKEKELGELIQSAVAAVLRTEER
                     IPSGIENLKFKRQSPLEPVRTEQLSVSFRSFDQEVVQETETKLSAII"
     gene            582..1169
                     /locus_tag="PLT73_10430"
                     /pseudo
     CDS             582..1169
                     /locus_tag="PLT73_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002398184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
BASE COUNT          344 a          282 c          298 g          271 t
ORIGIN      
        1 gatggatgcg gattccttgt tgttggatgt gaatgtccat ccttcgaaaa aagaaatcag
       61 gatcagcaaa gaaaaagaac tcggagaatt gatccagagc gcggtcgctg ctgttttgcg
      121 gaccgaagaa cgcattccga gcggcatcga aaatctgaag ttcaaacgcc aaagcccact
      181 tgaaccggta cggaccgagc agttgagtgt ctctttccgg tcattcgatc aagaggtcgt
      241 gcaagaaacg gaaacaaagc tgtcggcgat aatatgagaa tttccgatca ttggagacag
      301 catttgacaa cgggccggtt cataatggaa attcgcaacg tgaacaagct tttatgggtt
      361 ctgaagtagt cgcgccttcg ggagggctgc cgcctacaag tgaagaacaa gaagtttacg
      421 ctgttccgtt cactgatcaa ccaacgcagg accacgatga accgaatgct ccacatgtat
      481 ctatccatcc aaaaaatccg gttgcccatg aagaaccgat catgaaaacc gttcagaaag
      541 tcgaagcgga ggcagagaag ggaccggcga gcaagcgtcc gttcccggat ttgcactttt
      601 tcggccagat gcacggtact tacctgttcg cgcagaatga aaacggcctc tacatcatcg
      661 atcagcatgc tgcccaggaa cgaatcaaat atgaatatta tcgtgaggaa atcggcaagg
      721 tttccaccga tctccaaggg ttgctgatcc cgatcatcct ggattatccc gccaatgaat
      781 tccagttgat ccaggaaaat cgggaagagc tgcaagcgat gggcttgttc ctcgaaccat
      841 tcggccagaa cagtttcatc gttgaaaaac atcctgcctg gttcacacct ggtgaggagg
      901 aagacattct gaaggaactg gtggagatgt ttttggctga aggtgatatc tccatcaaaa
      961 agcttcggga agccactgcc atcatgatga gttgcaagcg ctcgatcaag gcgaatcatt
     1021 tcctaagcga caaagaagca cgccaactat tggtagatct tgccgaaacg gacaatcctt
     1081 acaattgccc gcacggtaga cctgtgttga ttcagttctc caaccaggat atggagaaaa
     1141 tgttcaaacg gatccaggac ccgcactgaa agcgtatcat ttcttatttc tgctt
//
LOCUS       DAOMUJ010000561         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31129_length_1186_cov_2.217507, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000561 DAOMUJ010000000
VERSION     DAOMUJ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31129_length_1186_cov_2.217507"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLT73_10435"
     CDS             <1..262
                     /locus_tag="PLT73_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRM20826.1"
                     /translation="SLIVRGEKISRIHCTVQYNGKDLGFNVTDHSRNGVFVNGVRIPY
                     NVPTYVSGKSIVALADGANKFQLIEAADEDDRSPALGADNEE"
     gene            252..>1186
                     /locus_tag="PLT73_10440"
     CDS             252..>1186
                     /locus_tag="PLT73_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20827.1"
                     /translation="MKSKKIIVAALVAVAAIVLAGCSPRSSMATLSTDSDSETAEVFS
                     LAKVLNLGTNYLTSLDYDNAILQYIDIIQHDPTNKEAYAGLYAAYAAQGKADEANKVF
                     EQAQEVFDDEADFLPGFLDDAKLVFESGGGNVPFQMLSEHFWNDLNSVFAKDVGEAWI
                     AADPQNADPYALLGAHYAALDDESGIEKLLQNAETNGVDFNTIDTKIKTNSDGTCTLT
                     VQINDFLKDESGQSASVSVKVDAKDNAKTVTQKVAEKAADSAASKVVEKSGLTGEAAG
                     IANSMAQEALRKGLGSAGSGQGTESSGTVTAPTYDP"
BASE COUNT          332 a          266 c          344 g          244 t
ORIGIN      
        1 gtcacttatc gtaagaggcg aaaaaatcag ccggattcac tgtacggttc aatataatgg
       61 aaaggatctt gggtttaatg ttaccgacca ttcccggaac ggggtattcg tcaatggggt
      121 gcgtattcct tataatgtgc cgacttatgt gtccgggaaa agtattgtcg cactggcaga
      181 tggtgcaaat aagtttcagc tgatagaagc ggcggacgaa gatgatagga gtccggcttt
      241 aggagcagac aatgaagagt aaaaaaatca tcgttgcagc actggtggca gtggctgcaa
      301 tagtactggc aggatgcagt ccgagaagct cgatggcaac attatccacc gattcagatt
      361 cggagacggc agaggtcttt tcgctagcca aggtcttgaa tcttggaacg aactacttga
      421 cctcgctgga ttatgataac gcaatcttgc agtatatcga tatcatccag cacgacccga
      481 cgaacaagga agcctatgcg ggcctgtatg ccgcttatgc agcacaggga aaagccgatg
      541 aggcaaacaa ggtctttgaa caggcgcagg aagtgtttga tgacgaggcg gactttcttc
      601 caggatttct ggatgatgca aagctggtct ttgaaagcgg tggtggaaac gttcccttcc
      661 agatgctttc ggaacacttt tggaatgact tgaattcggt ctttgcaaaa gacgtgggcg
      721 aggcgtggat tgcggcagac ccgcagaatg ccgaccccta tgccctgctg ggagcacact
      781 atgcggcact agacgatgaa agcggcattg agaaactgct ccagaatgcg gaaacaaatg
      841 gcgtcgattt caacaccatc gataccaaga tcaaaacgaa ctcggatgga acctgcacgc
      901 tgacggtaca gattaatgat ttcctgaagg atgaatcggg gcaatcggca tctgtaagtg
      961 tcaaggttga tgcgaaagac aacgctaaga cggtcaccca aaaggtggcc gagaaagcag
     1021 cggacagcgc agcaagcaag gtcgtggaaa aaagcggact caccggcgaa gcggccggca
     1081 tcgcaaattc gatggctcag gaggctctcc gaaagggcct cggcagcgcg ggaagcggac
     1141 agggaaccga atcgtccggg acagtgacgg cgcctaccta tgaccc
//
LOCUS       DAOMUJ010000562         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31494_length_1174_cov_1.566577, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000562 DAOMUJ010000000
VERSION     DAOMUJ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31494_length_1174_cov_1.566577"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>529)
                     /locus_tag="PLT73_10445"
     CDS             complement(<1..>529)
                     /locus_tag="PLT73_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM20828.1"
                     /translation="GSADLRDILASAEDVSLEEIMNDNVLSVRPETDQEEVSLIVSKY
                     DLKVIPVVNRRNVILGIITIDDIIDVIVEEQTEDLLMLSGVSKDEKVGNKISTSVVRR
                     LPWLVINLMTAFLASFTVGMFEDVIIQVVALAAAMPIVTGMGGNAGSQTLSVVIRSIA
                     LGEVDIKNDWKYVFNE"
     assembly_gap    530..539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<540..1042)
                     /locus_tag="PLT73_10450"
     CDS             complement(<540..1042)
                     /locus_tag="PLT73_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM20829.1"
                     /translation="MELNEMLLNAVENKNVSELKELFGDHYPVDVATALFEFEDEELA
                     ALLNLLEAKEIAAIIEEAEEELQQGMIGLLDSKKIVKFFAYMSPDDIADILGTLGVGQ
                     RKEILGMMKKSDSNEIQMLLGYGPDTAGGIMTTKYIALKSGLSMKDVLQEIRRIGPKT
                     EIIETIFV"
BASE COUNT          284 a          307 c          249 g          324 t
ORIGIN      
        1 cctcgttgaa aacatatttc caatcgtttt ttatatcgac ttcccccaag gcgatgctcc
       61 ggatgaccac ggataacgtc tgggagccgg cattcccgcc cattccggta acgatcggca
      121 ttgcagcagc aagcgcgacc acttgaataa tgacatcctc gaacatcccg actgtaaagg
      181 aagccaggaa ggcagtcatc aaattgatga ccaaccaagg cagccttctg acaacagaag
      241 tgctgatttt gttgccgacc ttttcatcct tgcttacccc tgacagcatc agcaagtctt
      301 ctgtttgttc ttcgactata acgtcgatga tgtcatcaat cgtgatgatg ccgaggatga
      361 cgtttcggcg gttcacgaca gggatgactt tcagatcgta tttggaaaca ataagcgaga
      421 cttcctcctg atcggtttcc gggcggaccg aaaggacatt gtcgttcatg atttcttcca
      481 agctgacatc ttcagcggag gccaaaatat cacgcaagtc ggctgacccn nnnnnnnnna
      541 caaaaatcgt ttcgatgatt tctgttttag ggccgatgcg tcggatttcc tgcagcacat
      601 ctttcatgct gagcccgctt ttcaaagcga tgtatttcgt ggtcatgatc cccccggcag
      661 tgtcaggtcc ataccccaaa agcatctgaa tctcattcga atcgcttttt ttcatcattc
      721 ccaagatttc tttgcgttgt ccgacaccga gcgttcccag gatatcggca atgtcatcgg
      781 gcgacatgta agcgaagaac ttgacgattt tcttcgagtc caacaagccg atcatgcctt
      841 gttggagttc ctcttccgct tcctctatga tggcggcgat ttccttcgct tccaacagat
      901 tgagcaaagc tgccaattct tcatcttcaa actcaaacag agccgtcgcg acatccactg
      961 gatagtgatc gccgaagagc tccttcaatt ctgaaacatt tttgttttct actgcattca
     1021 ataacatttc gttcaattcc atgtccatac ctccaatttg attcctcttt ttgaaggcat
     1081 aacagacgga ttcgagacga caagtttccg gtttatatta tctgcgattg ggctgaggat
     1141 acaaacagac ctgatggctg gctccgcacg gaac
//
LOCUS       DAOMUJ010000563         1170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31622_length_1170_cov_1.687892, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000563 DAOMUJ010000000
VERSION     DAOMUJ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31622_length_1170_cov_1.687892"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLT73_10455"
     CDS             complement(<1..165)
                     /locus_tag="PLT73_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM20830.1"
                     /translation="MPKRTDIQSILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIM"
     gene            complement(158..>1170)
                     /gene="carA"
                     /locus_tag="PLT73_10460"
     CDS             complement(158..>1170)
                     /gene="carA"
                     /locus_tag="PLT73_10460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRM20831.1"
                     /translation="VFNTGMTGYQETLSDPSYTGQIITFTYPLIGNYGINEDDFEGVH
                     PGLKGMVVHELAEHPSNFRCTKTLDVALKEFDVPGIYGVDTRSITKKIRNAGVMRGTM
                     VKNADNLDEIVASLKAYELPVDEIQMNSTKEIKKYAGEGPRVVLLDFGFKDNILAELL
                     KRGCEVILAPWNTSADEILAYKPDGIMLSNGPGDPTSVPEAIETIKQLIAQDNLPMFG
                     ICLGHQLISLASGATTYKLKFGHRGANHPVKDLETGKISLTSQNHGYAVNEDSLSETD
                     LVATHRALNDGTNEGVKHKTKPVFSVQYHPEAAPGPHDANYLFDHFVDMMKAVQGGKQ
                     HA"
BASE COUNT          257 a          274 c          281 g          358 t
ORIGIN      
        1 catgatcgta gcagggttgg agttgaccaa aatgactttg tatccttctt cttttaaagc
       61 caagcatgct tgcgttccgg catagtcaaa ttcagcggct tggccgatga tgattgggcc
      121 tgagccgatc actaaaatgg attgaatgtc ggtacgttta ggcatgttgt tttcctcctt
      181 ggactgcttt catcatatct acaaaatgat cgaacaagta attggcatcg tgcggtcccg
      241 gcgctgcttc cgggtggtat tgaaccgaga agaccggttt ggttttgtgt ttgacgcctt
      301 cgttcgtgcc gtcgttcaga gcacggtgag ttgcgaccaa gtctgtttcg ctcaggctgt
      361 cttcattcac agcatagcca tgattttgac tcgtcaggct gattttgccg gtttcgagat
      421 ctttcaccgg atgattcgca ccgcggtgac cgaattttaa tttgtaggtt gtcgcgccgc
      481 tcgccaatga gatcaactgg tggcctaagc aaataccgaa catcggcaga ttatcctggg
      541 caatcaattg cttgattgtt tcgattgctt ccggaacgga tgtcggatct ccaggtccat
      601 tgcttaacat gatgccatcc ggtttgtagg ccaaaatttc gtctgcgctt gtgttccagg
      661 gagccaaaat gacttcgcag cctcttttca ataattctgc aaggatgttg tccttgaagc
      721 cgaaatcaag caagacaacg cgtggacctt cacccgcata ttttttgatt tcttttgtcg
      781 aattcatttg gatttcgtca accggtaatt cataggcttt caacgatgcg acaatttcat
      841 ccaaattgtc cgcatttttt accattgtgc cgcgcataac gccggcattg cggatttttt
      901 tggtgatgct gcgtgtatcc acgccgtaga ttcctggtac atcaaattcc ttcaaagcta
      961 catccaatgt tttggtgcag cggaagttgc ttggatgttc cgccaattca tgcacgacca
     1021 tccctttcaa gcccggatgc acgccttcaa agtcatcttc gttgatgccg tagttgccga
     1081 tcaacggata cgtgaaagtg atgatctggc ctgtgtagga aggatcggac aaagtttctt
     1141 ggtaacctgt catccctgtg ttgaatacga
//
LOCUS       DAOMUJ010000564         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31636_length_1169_cov_3.196589, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000564 DAOMUJ010000000
VERSION     DAOMUJ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31636_length_1169_cov_3.196589"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLT73_10465"
     CDS             complement(<1..376)
                     /locus_tag="PLT73_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM20832.1"
                     /translation="MKKLVIEGNKPLNGEITISGAKNSTVALIPAAILADSPVILEGI
                     PNIQDVHSLIDILEDFNVGVSFDEDEGVITIDPTNMISIPMPTGKIKSLRASYYFMGA
                     ILSKFGEGVIGLPGGCFLGPRPI"
     gene            complement(818..>1169)
                     /locus_tag="PLT73_10470"
     CDS             complement(818..>1169)
                     /locus_tag="PLT73_10470"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM20833.1"
                     /translation="IDALAAALGSVHGPYSGEPVLGFDEMKEISELTHKPLVLHGGSG
                     IPEHQIKKAIEFGHSKINVNTECQQVFTAAVREKLATDANVYDPRKVIGPGKEAIIAV
                     VKERMQVFGSSNKA"
BASE COUNT          334 a          251 c          240 g          344 t
ORIGIN      
        1 cgatcggtct cgggcccaag aaacagcctc caggcagccc gatgacgcct tcaccaaatt
       61 ttgaaaggat agctcccata aaataatacg aagcacgtaa gcttttgatt tttccggtag
      121 gcatcggaat ggaaatcatg ttggtcggat cgatcgtgat gacgccctca tcctcatcaa
      181 aagacacccc gacgttgaaa tcctcaagga tatctatcag agagtgcaca tcctgaatgt
      241 ttgggatgcc ttccaagatg acgggggagt ccgccaaaat ggcagccgga atcagcgcaa
      301 ccgtactgtt tttcgctccg ctgatggtga tttcaccgtt caacggttta ttcccctcta
      361 taactaattt tttcataaaa aagcctcact tcatgtttag ttgtcgaacg aaaaagcgtc
      421 cggaaataaa tggaatcgct cacacgtctt gcgtagtgca gaaaaacgtt gctacgatac
      481 ctgcatacca ttatttttaa gttcatttga ttcattgtgt atcatctcta attttaacaa
      541 aaatataggg gcgattacta gttttttgtg cagacgttcg ttttccgcta catttctcaa
      601 tttgataaga tccagcagcg taaaaagaaa attcttgagc aatcattatg cttcaagtga
      661 atacagttgt acctacttta tgaccttatg gtaacacaaa ctcaggaata tatggaaacc
      721 ggggccgctg atgcacatgc ttttttgggg aaataaaaac aaaaaaagga gatccggaac
      781 ataggtccca gacctcctcg aagatcgttt gattaaatta agctttgttg cttgaaccga
      841 aaacttgcat tctttcttta acgactgcaa tgatcgcttc tttaccaggt ccgataactt
      901 tacgaggatc gtaaacgtta gcgtctgtag ctaatttttc tcttacagca gctgtaaaga
      961 cttgttggca ttcagtgttc acgttgattt ttgaatgtcc gaattcgata gcttttttga
     1021 tttggtgttc tgggattcct gaaccgccgt gcaatactaa tggtttgtga gtcaattcag
     1081 agatttcttt catttcatcg aaaccaagaa caggttcgcc agagtaaggt ccgtgtacag
     1141 aacctaaagc agcagccaat gcatcgata
//
LOCUS       DAOMUJ010000565         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31655_length_1169_cov_1.730700, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000565 DAOMUJ010000000
VERSION     DAOMUJ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31655_length_1169_cov_1.730700"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLT73_10475"
     CDS             <1..519
                     /locus_tag="PLT73_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM20834.1"
                     /translation="VTQWTYGQYLISGDKKAMTLRQTILNPGMIGAAIGLYIYISRLP
                     VPEFLWKSVDSLAALNTPLAMIILGSYIANSNFSELLAFPKAYWTTFLRLIVTALISI
                     GIIYLLPIDDYMVEIVLTIASCAPAATNTAILGRLFGGDYEYGARLVVLTTLLSLFTI
                     PIIVQFAQVLFG"
     gene            544..>1169
                     /locus_tag="PLT73_10480"
     CDS             544..>1169
                     /locus_tag="PLT73_10480"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRM20835.1"
                     /translation="MDKLLKGIVNFRRRDFESHRELFKGLKSSQKPHTLFISCSDSRI
                     DPNMITGTLPGELFIIRNIANIAPPYRVSSEYLATTSAIEYAVLALGVENIIICGHSN
                     CGGCAACLYPEGKLDDLPHTRKWLELIHPVRDRVLKEIPESEPEARAWMMEQGNVLEQ
                     LKHLISHPYIAERMEADKLQISGWHYIIETGEIFIYDPNLCEFLLANG"
BASE COUNT          282 a          296 c          274 g          317 t
ORIGIN      
        1 gtcacccagt ggacttatgg ccaatatctg atatccggcg acaaaaaagc gatgacgctg
       61 cggcaaacca tccttaatcc cggcatgatc ggggctgcca tcggtttgta catctacatt
      121 agtcgcctgc cggtacctga gtttctctgg aagtcagtcg acagcttggc agcgttgaat
      181 acaccactgg ccatgatcat tcttggaagc tacatcgcca acagtaattt ttcggaatta
      241 ttggcgttcc cgaaagcgta ttggaccact ttcctccgtt tgatcgtcac cgccctcatc
      301 tcgatcggca tcatttattt gctgcctatc gatgactata tggttgaaat cgtcctgact
      361 atcgcttcct gtgcaccggc tgcaacgaat accgccattc ttggccggct gttcggcggc
      421 gactacgaat atggagcgcg tctcgtagta ttgaccactt tattgtcctt attcaccatc
      481 ccgatcatcg tgcaattcgc acaagtgttg ttcggataat tatttcagaa agaaggtatg
      541 agcttggata agttattaaa aggtatcgtc aatttcagga ggagggattt tgaatctcat
      601 cgtgagttgt tcaaaggatt aaaaagttcc caaaaacctc ataccctctt catttcctgt
      661 tccgattcac gcatcgatcc gaacatgatt accggaacgt tgccaggcga attgttcatc
      721 atccgcaaca tcgccaacat cgctccgcct taccgggtct cttcggaata tttggcgacg
      781 acatccgcca tcgagtatgc tgtgctcgca cttggcgttg agaatatcat catctgcggc
      841 cattcgaact gcggcggctg tgctgcttgc ctgtatcccg agggtaaact ggatgatttg
      901 ccgcatacgc gcaagtggct ggaactcatt caccccgtcc gcgatcgtgt actgaaagaa
      961 attccggaat ccgaacccga ggcgcgtgcc tggatgatgg aacaagggaa tgtcttggag
     1021 cagctgaaac atttgattag ccatccttac atcgccgaaa ggatggaggc cgacaagcta
     1081 cagataagcg gttggcacta tatcatagag actggcgaaa ttttcatcta tgatcccaac
     1141 ttatgcgaat ttttgcttgc gaatggtta
//
LOCUS       DAOMUJ010000566         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31719_length_1167_cov_2.197842, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000566 DAOMUJ010000000
VERSION     DAOMUJ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31719_length_1167_cov_2.197842"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>207)
                     /locus_tag="PLT73_10485"
     CDS             complement(<1..>207)
                     /locus_tag="PLT73_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM20836.1"
                     /translation="TNIGPMLIYLVGGILIIEYGNTDLTIGDITVMVALLGRMYGPVN
                     SLLNIQVDMIRSMALFDRIFEYFDL"
     assembly_gap    208..221
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(225..755)
                     /locus_tag="PLT73_10490"
     CDS             complement(225..755)
                     /locus_tag="PLT73_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM20837.1"
                     /translation="MILANLISVLESYTNVWMAENITFDMRNKMYSHLQRMSHRFFTT
                     SKQGDIITRMTSDIGGVQSVLTGTWTSILQNFATLTVALVTMYTKSPLLATIGIVVVP
                     LFILPTKRVGKRRWEITLESRKHNDDINQILNETLSVSGQLLSKLFVTEAYEFERYQE
                     ANKQMIRLNIKESMAG"
     gene            complement(776..988)
                     /locus_tag="PLT73_10495"
     CDS             complement(776..988)
                     /locus_tag="PLT73_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20838.1"
                     /translation="MMGGNGFKGGRGGFLTEEEKQNSPKITLRLLKRMFSYLVPYWKQ
                     LIVSVLAIIISSVFGVFPTILTGRSA"
     gene            complement(985..>1167)
                     /locus_tag="PLT73_10500"
     CDS             complement(985..>1167)
                     /locus_tag="PLT73_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20839.1"
                     /translation="NRIFIQEKGDALLKKMKAVRQMERERINHSLTLEEQEQFVALCE
                     KLTKSLEDFRHQAGEQ"
BASE COUNT          260 a          322 c          278 g          293 t
ORIGIN      
        1 agatcgaaat actcgaagat gcgatcaaaa agtgccatcg agcggatcat atcaacctgg
       61 atgttcagca aggaattgac tggtccgtac atcctgccca ggagcgccac catgacggta
      121 atatccccga ttgtcagatc cgtgttgccg tattcaataa tgagaattcc gccgaccaga
      181 tagatcaaca tcggaccgat gttcgtgnnn nnnnnnnnnn nccattagcc cgccatgctt
      241 tccttgatgt tcaaacggat catctgcttg ttggcttcct gatagcgttc gaactcgtag
      301 gcttccgtga cgaaaagctt cgaaagcaat tgcccgctga cactgagcgt ctcgttcagg
      361 atctggttga tgtcgtcatt gtgcttccgt gactcaagcg ttatttccca acgccttttg
      421 cccacgcgct tcgtcggcag aatgaacaat ggcaccacca caatgccgat tgtcgccagc
      481 aacggacttt tcgtatacat cgtgacgagc gcaaccgtca acgtcgcgaa attctgcagg
      541 atgctcgtcc acgtgcccgt cagcacgctc tgcacgccgc cgatatcgct ggtcatgcgg
      601 gtgatgatgt cgccctgctt gctggtcgtg aagaaccggt gcgacatccg ctgcagatgg
      661 ctgtacatct tgttgcgcat gtcaaaggtg atattttccg ccatccagac attggtatag
      721 ctctccagta cgctgatcag attggcgagg atcaacactc cgaacgaaag gccgatcagg
      781 cggatcggcc cgtcaggatc gtcggaaaaa cgccgaatac cgacgagatg atgatcgcca
      841 acactgatac gataagttgt ttccaataag ggacgaggta gctgaacata cgcttcagta
      901 gccgcaacgt gattttgggg ctgttctgtt tctcctcctc cgtcagaaag cctccgcggc
      961 cgcctttgaa cccgttcccg cccatcattg ctcacctgct tggtgtctga aatcctccaa
     1021 ggattttgtc agtttttcgc agagcgcaac gaattgctct tgctcctcaa gcgtcaagct
     1081 atgattgatg cgctcccgtt ccatctggcg tactgccttc attttcttca ataaagcatc
     1141 ccccttttct tggatgaaaa tccggtt
//
LOCUS       DAOMUJ010000567         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31774_length_1166_cov_1.078308, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000567 DAOMUJ010000000
VERSION     DAOMUJ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31774_length_1166_cov_1.078308"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1166
                     /locus_tag="PLT73_10505"
     CDS             <1..>1166
                     /locus_tag="PLT73_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20840.1"
                     /translation="TGETYEEAYDVLFLAPGANAVRPRSIAGIDSKHVFVMKTVPDVA
                     ALTAYTKANEVKDVAVVGGGYIGLEVAENLKMAGYNVSLIEAQDQIMAPLDYDMVQII
                     NREIMKNGVDLILKDSVQAIGSDEVTLASGRTVKAQAVVMAIGVSPENALAKQAGLEL
                     GVAGSIKVNHHYQTSNPHIYAVGDAIETTCFFTGQKTQLTLAGPAQRQARAAADHVYG
                     RTYLNTGVIGSSSVKMFGLNSANTGLNEKQCQARGIAYDFAYIIPKDKVSIMPGAENL
                     FFKLIFAVPSGKILGAQAVGRGNVDKRIDVIATAIMLNANLEDLKELELTYSPHFSTA
                     KDVVNHAVLVGLNILNGEFKKVPVTKVRELVESGAMIIDAREEAFADLNRAIEL"
BASE COUNT          303 a          269 c          314 g          280 t
ORIGIN      
        1 agacaggcga aacgtatgaa gaggcgtacg atgtcttgtt cttggcgccg ggtgcgaatg
       61 ccgttcgtcc gcgttccatc gcagggatcg attccaagca tgtattcgtc atgaagacgg
      121 tgccggatgt agctgctctt acggcctata ccaaggctaa cgaagtcaaa gatgtggcgg
      181 tcgtgggcgg cggctatatc ggtcttgaag tcgctgagaa cctgaaaatg gcgggctaca
      241 atgtttcctt gatcgaagcg caagaccaaa tcatggcgcc attggattat gatatggtcc
      301 aaatcatcaa tcgcgaaatc atgaaaaatg gcgtagatct gattttgaaa gacagcgtcc
      361 aagcgattgg ttcggacgaa gtgacattgg cttccggtcg taccgtcaaa gcgcaagccg
      421 tcgtgatggc catcggagtg tcgccggaaa atgcgttggc gaaacaggct ggtctggaat
      481 tgggtgtcgc tggttccatc aaagtgaacc atcactatca aacaagtaat ccacatattt
      541 atgccgttgg ggatgcaatc gaaacgactt gcttctttac aggtcaaaaa acgcaactga
      601 cacttgccgg tcccgcgcaa cgtcaagcac gcgccgctgc tgatcacgta tacggccgaa
      661 cttatcttaa cacaggcgtt atcggttcct cgagcgtgaa aatgttcggt ttgaattctg
      721 cgaacactgg cttgaatgag aagcaatgcc aagcacgcgg tatcgcctac gattttgctt
      781 acatcatccc gaaagataag gtcagcatca tgcctggtgc ggagaacttg ttcttcaaat
      841 tgatctttgc tgtgccatca ggaaaaattt tgggggcaca agcagtcgga cgcggaaatg
      901 tcgataaacg gatcgatgtc atcgctacgg caatcatgtt gaacgccaat cttgaggact
      961 tgaaagagtt ggaattgacc tactccccgc acttttccac agccaaggat gtcgtgaacc
     1021 atgccgttct tgtcgggttg aatattttga atggcgaatt caaaaaagtg ccggtaacca
     1081 aagtgcgtga attggttgag agcggtgcga tgatcatcga tgcgcgcgaa gaagcctttg
     1141 ccgatctgaa ccgcgccatt gaactc
//
LOCUS       DAOMUJ010000568         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31896_length_1162_cov_2.066847, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000568 DAOMUJ010000000
VERSION     DAOMUJ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31896_length_1162_cov_2.066847"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1162
                     /locus_tag="PLT73_10510"
     CDS             25..>1162
                     /locus_tag="PLT73_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase-like protein"
                     /protein_id="HRM20841.1"
                     /translation="MARAFYEAYQVKTNLISPMIIGPIGNSRIIKHYIQPGIDDLDLF
                     FETIHQIGRDYPNMKKIIFGADDRYAELLIRTKDRFGDDWIIPYVDEPVFLRATNKES
                     FYAICEKTGVPYPKTVVMDEFSLDLPFEFPIIIKPSNAIMYQGLHFEGKEKVFIGRDE
                     KDLERIYHLVRDNGYTDNLILQEYVPGDDTYLGVVTVYTSPRDKEIKLIAFGHILLED
                     HTPSAIGNHLTIWAREEQKIVASVQKLIAETEFYGFSNFDVKYDKRKDDYVFFELNGR
                     LGVSNYYVTASENNVAKYYVEDFIAKKKLGLTINTNEALFTMTPKKLLLKYIESDVLR
                     KKVKELYKDGKVVHPLDAPFEVSPKRKFYVLASKFNYYKKFKEYP"
BASE COUNT          346 a          243 c          267 g          306 t
ORIGIN      
        1 ggggggaacc gaggagccta tagtttggcg agagctttct atgaagcgta ccaagttaaa
       61 acgaatctca tcagcccgat gattataggg cctatcggca attcccgcat catcaagcat
      121 tacattcaac cgggaatcga tgacctggat cttttttttg aaaccatcca tcagatcggt
      181 cgggactatc cgaatatgaa aaaaatcatc ttcggggcag acgatcgcta tgcggaactg
      241 ctgatccgga cgaaagaccg attcggtgat gattggatca ttccgtatgt ggatgaacca
      301 gttttcctac gggcgaccaa taaggaaagt ttttacgcca tttgtgaaaa aacaggcgtt
      361 ccctatccga agactgtagt catggatgaa ttttcccttg atttgccgtt tgaatttccg
      421 atcatcatca agccttcgaa tgccatcatg taccaggggt tgcattttga gggtaaagaa
      481 aaagtcttca tcggacgcga cgagaaggat cttgaacgca tctaccattt agtgcgtgac
      541 aatggctata cggacaacct gatcctgcag gagtacgttc ctggcgatga cacctatctt
      601 ggcgtcgtga cggtgtacac atcgccacgt gacaaggaaa ttaagctgat tgcattcggc
      661 catatcctct tggaagacca tacgccatcc gccatcggaa accatctgac gatttgggcc
      721 agggaagagc agaagattgt tgcttctgtc caaaagttga ttgctgaaac cgagttttat
      781 ggtttttcca attttgatgt gaagtacgat aagcgcaaag atgactatgt tttctttgaa
      841 ctgaacggtc ggcttggcgt aagcaactac tacgttactg ccagcgaaaa taatgtcgcg
      901 aaatactatg ttgaagactt catcgccaag aaaaaacttg ggttgaccat caacacgaat
      961 gaagctttgt tcacgatgac accaaaaaaa cttttgctga aatacatcga gtctgatgtt
     1021 ttaagaaaaa aagtaaaaga gttatacaag gacgggaaag tcgtgcaccc attggatgcg
     1081 ccttttgaag tcagcccaaa aagaaaattt tatgtgttgg cttccaaatt taattattac
     1141 aaaaaattca aagagtatcc gc
//
LOCUS       DAOMUJ010000569         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31899_length_1162_cov_1.822945, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000569 DAOMUJ010000000
VERSION     DAOMUJ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31899_length_1162_cov_1.822945"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLT73_10515"
     CDS             complement(<1..631)
                     /locus_tag="PLT73_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HRM20842.1"
                     /translation="MSFKFKLPALGEGIMEGEIVSWSIKEGDTINEDDTLLEVQNDKS
                     VEEIPSPVSGKVLKIVAPVGTVAVIGDVLVEIDAPGHEDEGSAAPAPATASAASTPAA
                     PAASTGGLFQFKLPALGEGIMEGEIVSWPIKEGDMINEDDTLVEVQNDKSVEEIPSPV
                     TGKVVRIVAPVGTVAVIGDVLVEIDAPGQNGPASATPKAAAAPAAASNAP"
     gene            complement(738..>1162)
                     /locus_tag="PLT73_10520"
     CDS             complement(738..>1162)
                     /locus_tag="PLT73_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM20843.1"
                     /translation="EEAYTVPIGKAAITKEGSDVSVITYGAMVREAIKAAETLEKEGI
                     SVEVIDLRTVAPLDIETIIASVEKTGRVVVVQEAQRQAGVGAMVMSEISERAVLSLEA
                     PIGRVAAPDTVFPFGQAENIWLPNATDIENKVRETYHF"
BASE COUNT          280 a          287 c          252 g          343 t
ORIGIN      
        1 ctggtgcatt tgaagctgct gcaggtgctg ccgctgcttt tggtgttgct gaagccggac
       61 cgttttggcc tggagcatcg atttcaacca acacatcacc gatgaccgca actgtaccta
      121 ctggcgctac gatgcggaca actttacctg tcactggaga tgggatctct tcaacagatt
      181 tgtcgttctg aacttcaacc aaggtgtcat cttcgttgat catatcgcct tccttgatag
      241 gccaagaaac gatttcgcct tccatgatac cctcgcctag agcaggcaat ttgaattgga
      301 acagtccgcc tgttgaagct gcaggtgctg ctggagttga tgccgctgaa gctgttgctg
      361 gtgcaggagc tgcagaacct tcatcctcgt gacctggagc gtcgatttcc accaatacat
      421 ctccgatgac cgcaaccgta cctaccggcg caacaatctt caaaactttc ccagatactg
      481 gggatgggat ttcttcgact gatttatcat tttgaacttc taataatgtg tcatcttcat
      541 tgatggtgtc gccttctttg attgaccaag aaacgatttc accttccatg ataccttcgc
      601 ctagagcagg caacttgaat ttaaaagaca ttttcttaac ttcccttcat ttgtctattt
      661 tttgatttcc gacgcacaaa agcggacaaa tacaattcta tgggatatgg aattgtattt
      721 gtccaagctt tcagctctta gaaatgataa gtttctctta ctttgttctc gatatcagta
      781 gcattcggta accaaatgtt ttctgcttga ccgaacggga agacagtatc tggagcagct
      841 acacgtccga taggagcttc taacgataag actgcacgtt cagaaatttc ggacatgacc
      901 attgcgccta caccagcttg acgctgcgct tcttggacaa ctacgacacg gcctgttttt
      961 tcaaccgagg caatgatcgt ttcaatatca agtggagcaa ccgtacgcaa atcgattact
     1021 tcaaccgaaa tgccttcttt ttcaagtgtt tctgcagctt tgatagcttc gcggaccatt
     1081 gcgccgtaag tgatgacaga cacgtcagat ccttcttttg tgatggcagc tttgccgata
     1141 ggaaccgtgt acgcttcttc ag
//
LOCUS       DAOMUJ010000570         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_31902_length_1162_cov_1.703704, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000570 DAOMUJ010000000
VERSION     DAOMUJ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31902_length_1162_cov_1.703704"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..680
                     /locus_tag="PLT73_10525"
     CDS             69..680
                     /locus_tag="PLT73_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20844.1"
                     /translation="MNIPMKAENFTAVGSFIKNVDFDGTEVLRVTKDPKIEAFDEPTF
                     ARLVGTKFKNGTIEGKVLSRLLPDAPEFARGFLGVAFRIDENNEHFESFYIRPTNGRN
                     ESQLRRNRATQYFSYPDYKFDRFRSESPGEYESYADMGLDEWIDLKIKVDGEHAELYV
                     NNSKHPVLVVNDLKLGPDAEGDVALWVEEGTEGFFKDLKINPS"
     gene            880..1161
                     /locus_tag="PLT73_10530"
     CDS             880..1161
                     /locus_tag="PLT73_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20845.1"
                     /translation="MKMQFWSKTTTGKWAASLTMAFIVLMAIKMTAIGATIRLPLPTP
                     VLALLGVIGFIMGLIAIIKYKDRALLTLLSIPVGIIIIAWTLAEIAFPH"
BASE COUNT          351 a          237 c          302 g          272 t
ORIGIN      
        1 gattctagaa tcgtgctaga ctgatgataa gaacagcata aatcactcat gtaaagggga
       61 agaaaagcat gaacatacct atgaaagcag aaaactttac cgcagttggt tcctttatta
      121 aaaacgttga ttttgacgga acggaagtac ttagggtcac gaaagatccg aaaatcgaag
      181 cttttgatga accaaccttt gcaaggttgg tgggcactaa attcaagaac ggcacgatcg
      241 aagggaaggt attgagccgt ctcttgcctg acgcgcccga attcgccaga ggattcctcg
      301 gtgttgcttt ccgcatcgat gaaaacaacg agcatttcga atcgttctat attcgtccga
      361 cgaacggcag gaatgaaagc caactgagaa ggaaccgcgc cacacaatat ttctcctacc
      421 ctgactacaa attcgaccgc ttcagatccg aaagtccagg cgagtacgaa tcctacgctg
      481 acatgggatt ggatgaatgg atcgatttga agatcaaagt ggacggagaa catgccgaat
      541 tgtatgtgaa caattcgaag catcccgtgt tggtggtcaa cgatctgaag ctcgggccgg
      601 atgcggaagg tgatgttgcg ctttgggtcg aggaaggcac ggaagggttt ttcaaggatt
      661 tgaagatcaa tccgagctag aacagtgttg ttaaagaaga ttctgcggcg aggaatggat
      721 tcggtttata aaaaatagaa cgcaataacc aaaaggaaag cctgtggatc atagaagctg
      781 atagggtggt tgtgcccaca gaaattaacg ggaagctcta tcgtgaggga taacaagcaa
      841 cggcagttta ccttgaacaa gaatatggag gaaacgatca tgaaaatgca attttggtcc
      901 aaaacgacaa ccgggaaatg ggcagcgagt ttgactatgg cattcatcgt gctgatggcc
      961 atcaaaatga cggcaattgg agctacaatc agactgcctc taccgacgcc cgtccttgca
     1021 ttgctgggag tgatcggttt catcatgggg ctgatagcca ttatcaaata caaggacaga
     1081 gccttgctga cgttgttgtc cattccggtt gggataatta tcatcgcatg gacactcgct
     1141 gaaattgctt tcccgcactg ac
//
LOCUS       DAOMUJ010000571         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32120_length_1156_cov_1.938238, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000571 DAOMUJ010000000
VERSION     DAOMUJ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32120_length_1156_cov_1.938238"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLT73_10535"
     CDS             complement(<1..232)
                     /locus_tag="PLT73_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRM20846.1"
                     /translation="MVDFNKDAKDLLEYIGGPDNISAVSHCATRMRFVLNDPAAANIK
                     KIEDIPAVKGTFTQAGQFQVIIGNEVPQFYNEF"
     gene            complement(464..>1156)
                     /locus_tag="PLT73_10540"
     CDS             complement(464..>1156)
                     /locus_tag="PLT73_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM20847.1"
                     /translation="ARVPAAWVVPLPEGLSLKEAMIIGTAGYTAAESVDALVQQGIQS
                     QTGNVLVTGASGGVGGMAIAMLKRLGYTVEAVSRKKADCSDYLKQLGAEAVLHPDEVA
                     MEKKRPLAQQRWAAIVDPVGGPMLPDLLAQLHYGGCLALSGNAGGIAFEATVLPFILR
                     GVKLVGIDSVNHPYAERIKLWERMASGLKPENLELLVEQEITLEDLPAAFGKIMDGKM
                     HGRTLVKVKQGI"
BASE COUNT          262 a          350 c          248 g          296 t
ORIGIN      
        1 cgaactcgtt ataaaattgt ggcacttcat taccgatgat gacctgaaat tgtcctgctt
       61 gggtaaaggt tccttttact gcgggtatgt cttcgatctt cttgatgttg gccgcagccg
      121 gatcattcag aacgaaacgc atccgagtgg cacagtgact gactgcagaa atattatcgg
      181 ggccaccgat atactccagc aaatcctttg catctttgtt gaaatctacc attttttctc
      241 ctcctctact tgtatataca tgttcctgac atttctattt taccgcgaac cattccgcaa
      301 tgcaagcggt ttcctcccgc aatacattta cttgtataga caagtttccc gttttccgca
      361 ttttatgaag cgttatcagg ctggacaaca cccttaaaaa aaacggcaaa agaatcggat
      421 gatgcctgat tccttgccgt ttttgatggt tatgctaagc cgatcatatc ccttgtttca
      481 ctttcaccaa agtccgccca tgcatctttc cgtccatgat cttcccgaaa gccgctggta
      541 aatcctccaa cgtgatttcc tgttcgacca gcaactccag attttccggc ttcagaccgc
      601 ttgccatgcg ctcccaaagc ttgatgcgtt cagcgtaggg atggttcacc gaatcgatcc
      661 caacaagctt gacgcccctc aggatgaaag gcaaaacggt cgcttcgaat gcgattccgc
      721 cggcatttcc gctcaaagcc agacagccgc cgtaatgcag ctgcgccagc aagtccggca
      781 gcatcggacc gccgactgga tcgacgatcg ccgcccaacg ctgctgagcc aatggccgct
      841 tcttctccat tgccacttca tcgggatgga gaaccgcttc agcacccagt tgcttcaggt
      901 aatccgagca gtccgctttt ttccgggaca cagcttcgac cgtataccct aggcgcttca
      961 gcatagcgat tgccatgccg ccgacaccac cggaagcgcc agtcaccaat acattccccg
     1021 tttgcgactg gatgccttgt tgcaccaaag catccactga ttccgccgct gtataaccgg
     1081 ccgttccgat gatcatcgct tccttcaggc tcaacccttc cggcaacggc acgacccaag
     1141 ctgcagggac ccgggc
//
LOCUS       DAOMUJ010000572         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32160_length_1155_cov_1.765455, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000572 DAOMUJ010000000
VERSION     DAOMUJ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32160_length_1155_cov_1.765455"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLT73_10545"
     CDS             <1..348
                     /locus_tag="PLT73_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HRM20848.1"
                     /translation="VDEFLPYKGTFFWDVQHGLKALLKDIPDWSVKSGLITEKIVPAL
                     LDRPNPVPVIVGTTRLFNSKYKNHWVVVYAYGYNEDDKLFYKAYDNHGRYQAILPASQ
                     TIGCVWLEERKNN"
     gene            379..>688
                     /locus_tag="PLT73_10550"
     CDS             379..>688
                     /locus_tag="PLT73_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006267503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20849.1"
                     /translation="MREYDFIAIGGGSGGIATMNRASEYGAKTAVIEGKLLGGTCVNI
                     GCVPKKIMWYASQISEAIHKYGPDYGFDTGAHKIDFPTLLKNRDAYVERSRNSYQAGF
                     E"
     assembly_gap    689..698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <699..>1155
                     /locus_tag="PLT73_10555"
     CDS             <699..>1155
                     /locus_tag="PLT73_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20850.1"
                     /translation="EGFAKFVDNHTVEVNGELIRSKHILIATGAKPIRPRIPGAKLGD
                     VSDTFFEWTELPEKVAVVGAGYIAIELAGVLHNLGVDTHLYVRYDKPLRKYDDSIIEG
                     LMEEIEKDGPTLHTHAVSKAVEKNADGTLTLHLEDGRSEVFDKIIWAIGR"
BASE COUNT          322 a          264 c          308 g          251 t
ORIGIN      
        1 gttgatgaat ttctgcccta caaggggact ttcttctggg acgtgcagca cggactgaag
       61 gctttgctta aggacattcc ggattggtcc gtaaaatcgg gactcatcac ggaaaaaatc
      121 gtgccggcct tgttggaccg tccgaatccg gtaccggtca tcgtcggaac cacgcggctt
      181 ttcaacagta agtacaaaaa ccattgggta gtcgtctatg cttatggcta caacgaggac
      241 gacaagcttt tctataaagc gtatgataac cacggccgct accaggccat tttgccggca
      301 tcgcagacga ttggatgtgt ctggctggaa gaaagaaaaa ataattgaga atactataga
      361 tagtaacgga ggaacaaaat gagggaatat gattttatcg ccatcggagg cggcagcggc
      421 ggcatcgcaa cgatgaaccg tgcgtcagaa tatggtgcca aaacagctgt tattgaaggg
      481 aaattattgg gtggaacgtg cgtgaacatc gggtgcgtac cgaaaaaaat catgtggtat
      541 gcatcccaaa tatcggaagc aatccacaaa tacggaccag attatgggtt tgatacaggt
      601 gcacacaaaa tcgacttccc gaccttgctg aagaaccggg acgcctatgt agagcgttcc
      661 cgcaactcct atcaagcggg cttcgaacnn nnnnnnnntg aaggctttgc caaattcgtc
      721 gataaccata ctgttgaggt gaacggcgag ctgatccgtt ccaaacatat cttgatcgcg
      781 actggtgcaa aaccgatccg tccacggatt cctggcgcaa agctaggcga cgtttccgat
      841 acattcttcg aatggaccga actgcccgag aaagtcgcag tcgtgggagc cggctacatc
      901 gccatcgaat tggccggcgt gctgcacaat ttgggagtgg atacgcatct ttatgtccgc
      961 tatgataagc cgttgcgcaa atacgatgac agcatcatcg aaggactgat ggaggaaatc
     1021 gaaaaggatg gtccgacatt acatacgcat gccgtatcga aagctgtcga aaagaatgca
     1081 gacggcactt tgacgctgca cctagaggac ggcagatcgg aagtcttcga caaaatcatc
     1141 tgggcgatcg gacgg
//
LOCUS       DAOMUJ010000573         1152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32271_length_1152_cov_2.273473, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000573 DAOMUJ010000000
VERSION     DAOMUJ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32271_length_1152_cov_2.273473"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..>1152)
                     /gene="mnmH"
                     /locus_tag="PLT73_10560"
     CDS             complement(270..>1152)
                     /gene="mnmH"
                     /locus_tag="PLT73_10560"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRM20851.1"
                     /translation="VVGTLYVAGRIDEAKSHGIQVISPRLPEMFSLFQEYTKAYDQVV
                     IFCSRGGFRSNSIFSLLKSLGMNVHRMEGGYKNYRSTINNLIPVLFEKIHFVTLYGNT
                     GTGKTAILEQLKKRGANVLDLEACANNRGSVFGGVGLKKKQPHNQKMFESLLVESATG
                     WKEPLVVFTEGESKRIGQAVLPPALIDAMQKGINLNIEASLDYRIGQIKKDYLHSNET
                     ELLAALDRLKSYLNEARVEGYKEQVRQHDFEAVIADLLVNPRYNFNKKEFSAVFRNED
                     AAATAEAIMEWMKQRND"
BASE COUNT          272 a          296 c          275 g          309 t
ORIGIN      
        1 catccatcaa gcttttggaa ctgaaaaaaa cggtatggtc cgcttcagct tctccagctt
       61 caacacggtg gaggaaatcg actacgcagc caaagcgttg aggagcttgg cggaagaagg
      121 gtaacaatca aaagttgtcc gataaccggg aacaatcgga cagccacagc aagagcggct
      181 taatatagcg agtatcttct tgtaaaaaat aaaatagagc agccacccca atcatcaagg
      241 gcagctgctc tgttttattt tctctctagt caatcgttcc tctgcttcat ccattccata
      301 atggcttcgg ccgttgctgc ggcgtcttcg ttgcggaaaa cggcggaaaa ttcctttttg
      361 ttgaagttgt agcggggatt caccaataga tctgcgatga cagcttcgaa atcatgctgg
      421 cgcacttgtt ccttgtagcc ctcgacacgc gcctcattca gataagattt caagcggtcc
      481 agggccgcga gaagctctgt ttcattgctg tggagataat cctttttgat ctgtccgatc
      541 cggtaatcca atgaagcctc gatgttcagg tttatgcctt tttgcatcgc atcaatcaac
      601 gcaggcggca atacggcctg tccgatccgc ttgctttcgc cttcagtaaa gacgaccagc
      661 ggttccttcc agccggttgc gctctcgacc aacagacttt cgaacatttt ttggttgtgc
      721 ggctgtttct tcttcagacc gacgcctccg aagacggacc ctctgttgtt ggcgcaggcc
      781 tccaaatcaa gcacattggc tccacgtttc ttcagttgct ccaggatggc tgttttcccg
      841 gtcccagtgt tgccatagag ggtcacaaaa tggatctttt cgaacaatac gggaatcaga
      901 ttattgatcg tgctgcggta gtttttgtag ccgccttcca tccggtggac attcatccct
      961 aaagatttaa gcaaggaaaa aatcgaattg ctgcggaatc ctcccctgct gcagaagatg
     1021 acgacttggt cgtaggcctt cgtgtattcc tggaagaggc tgaacatctc cggcaatctt
     1081 ggagaaatga cttggatacc gtgacttttc gcttcgtcga ttcggccggc aacataaagc
     1141 gtcccgacca ct
//
LOCUS       DAOMUJ010000574         1150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32352_length_1150_cov_1.700457, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000574 DAOMUJ010000000
VERSION     DAOMUJ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32352_length_1150_cov_1.700457"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..>1150)
                     /locus_tag="PLT73_10565"
     CDS             complement(439..>1150)
                     /locus_tag="PLT73_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HRM20852.1"
                     /translation="VIPYLERLTGGEALMGILTNYATDCLATAECRIPSSLLGRGSLS
                     GDEVRDRLIEAYQFAYVDPYRAVTNNKGIMNGIDAIVLASGNDWRAISAGAHAYASRS
                     GQYRSLTTWKKAENGDLLGSLTLPLPIGAVGGSINFHPAAKMTKEILGYRSASELESI
                     IASVGLAQNFSAIKALVTEGIQKGHMGLHSRSLAISAGAVGKEIEQLSERLKGEKNMN
                     LATAQALLAQIRGADAVD"
BASE COUNT          269 a          300 c          309 g          272 t
ORIGIN      
        1 acggacagaa gtgagcgtca aagaatatga agagatctat tctcgtgttt tgccgattga
       61 tggttcaagc atcgaattgg atgtggatgc tgatcctgcg agtatctgta ttgaaggaat
      121 ggaaaacaac aaacgcatct atctgaacaa aaccagatga tcgcatgaat gaagacagca
      181 caccgtcagc cagacaactg gtggacggtg tgctgttttt gatttttaat ggttctggcg
      241 gccggcacct ccggacaagc cgtccttggc cggaggtgaa acgaatttcg gccttgtact
      301 ccggtgaggc cgccgtgacc ggagaataaa acaaaatccg gtacctctac tccggcgagg
      361 agagtctcac cggaggaaaa acgggaatcc agcccggcag ctcgctctgg aaccctccgc
      421 catcgatctt gttgtgactc aatcgactgc atccgcaccg cggatttgcg ccaaaagtgc
      481 ttgtgctgtc gccaaattca tatttttttc gcctttaagc cgctctgaaa gctgttcgat
      541 ttctttaccg accgcgccag cgctgattgc caaggaacgc gaatgcagac ccatatgtcc
      601 tttttggatg ccttccgtca ccagcgcttt gatggcggag aaattttgcg ccagtccgac
      661 cgatgcgatg atggactcta gttcggatgc cgaacggtag ccgagaattt ctttcgtcat
      721 tttcgcggca gggtggaagt tgatggatcc gcccacagct ccgatcggca acggcaacgt
      781 cagacttccg agcagatctc cattttccgc ttttttccag gttgtcagcg aacgatactg
      841 tccgctgcga gaggcatagg catgcgctcc agcagaaatg gcgcgccagt cgtttccgga
      901 tgccagcacg atggcgtcga tgccgttcat gatgcctttg ttattggtga cggcgcgata
      961 aggatcgacg taggcaaact gataggcttc aatcaatcgg tcgcgcactt catcaccgga
     1021 aagacttcct ctgcccaaga gacttgatgg tatccgacat tcggcggtcg ccaagcagtc
     1081 ggtcgcgtaa ttggtcagga ttcccatcaa agcttcgccg ccggtcaaac gctccaagta
     1141 agggatgacg
//
LOCUS       DAOMUJ010000575         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32399_length_1148_cov_2.569991, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000575 DAOMUJ010000000
VERSION     DAOMUJ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32399_length_1148_cov_2.569991"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..>1148)
                     /gene="nrdD"
                     /locus_tag="PLT73_10570"
     CDS             complement(7..>1148)
                     /gene="nrdD"
                     /locus_tag="PLT73_10570"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM20853.1"
                     /translation="KKGTNLETHDPNYDIKQLAITCATKRMYPDVLMYDKIVELTGSF
                     KTPMGCRSFLQGWEDENGNEVNSGRMNLGVVTLNIPRIALESEGNPEAFWNIFEERLA
                     ICKDALVYRVERTKEATPTNAPILYQHGAFGKRLGETDKVDELFKNKRATVSLGYIGL
                     YEAATVFYGADWETNPEAKAFTLEIVKKMKEKTDAWGNQYGYHFSVYSTPSESLTDRF
                     CSMDTEKFGIIADITDKEYYTNSFHYDVRKNPNPFEKIAFEMEYPQYCSGGFIHYCEY
                     PNLQQNPKALETVWDFAYDKVGYLGTNTPIDHCLECGFEGDFNPTERGFQCPECGNTD
                     PQTCDVVKRTCGYLGNPQIRPMVKGRHKEISARVKHLHADCKSEQ"
BASE COUNT          240 a          289 c          281 g          338 t
ORIGIN      
        1 ttctgattac tgttctgact tgcaatccgc atgcagatgt ttgacgcggg cggaaatttc
       61 tttatggcga cctttcacca tcggacggat ctgcggattg ccgagatagc cgcatgtgcg
      121 ttttacgacg tcgcatgtct gcggatcggt attgccgcat tccgggcatt ggaatccgcg
      181 ttcggtcgga ttgaagtctc cttcgaaacc gcactccagg cagtggtcga tcggcgtatt
      241 cgttcccaaa tagccaactt tgtcgtaggc gaaatcccag actgtttcca acgctttcgg
      301 attttgctgc aagttcgggt attcgcaata atggataaag ccgcctgagc aatattgcgg
      361 atattccatt tcgaaggcga ttttttcgaa aggattcgga ttcttgcgga catcgtaatg
      421 gaagctgttt gtgtagtatt ccttatccgt gatatcagcg atgatgccga atttctctgt
      481 gtccatgctg cagaagcgat cggtcaaact ttcgctcgga gtcgagtaga cgctgaaatg
      541 ataaccgtat tggttgcccc aagcatccgt tttttccttc atcttcttga cgatctccaa
      601 agtgaaggct tttgcttccg gattcgtttc ccaatctgct ccgtagaaaa cggtagccgc
      661 ttcatacaat ccgatatatc ccagagagac tgtagcgcgt ttgtttttga atagttcatc
      721 gaccttatcc gtttcaccca ggcgtttccc gaaggctccg tgctggtaca ggatcggtgc
      781 gttcgtaggc gtcgcttctt tggtgcgttc gacacggtag acgagggcat ccttacagat
      841 ggccaagcgt tcctcgaaga tgttccagaa tgcttccgga ttcccttccg attccaaggc
      901 gattctaggg atgttcaacg tcacgacccc aagattcatc cgaccggaat tgacttcatt
      961 tccgttttcg tcttcccagc cctgcaggaa ggaacggcaa cccatcggtg tcttgaaact
     1021 gcctgtcaat tcaacgattt tatcgtacat cagcacatcc ggatacatcc gtttcgtggc
     1081 acaagtgatg gccaactgct tgatgtcata gttgggatcg tgcgtctcta ggttcgtgcc
     1141 ttttttca
//
LOCUS       DAOMUJ010000576         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32647_length_1142_cov_1.246550, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000576 DAOMUJ010000000
VERSION     DAOMUJ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32647_length_1142_cov_1.246550"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLT73_10575"
     CDS             <1..270
                     /locus_tag="PLT73_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HRM20854.1"
                     /translation="DICFIGAASLEKEGIFFKEPNNALIKQKVVKQSRQVVLVAENQK
                     FSKEAQYKGANYQDIDCFITDRTLASKQQAYFGDQTEIIFEEETI"
     gene            267..1079
                     /locus_tag="PLT73_10580"
     CDS             267..1079
                     /locus_tag="PLT73_10580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM20855.1"
                     /translation="MTKVELVFSDIDGTLLDSKHEVSPEANQIIQDLMKQGTKIILAS
                     ARPPGAMVSIADEVRLSSPLVCFNGALITQYKEGSFDDLYSLTLERLDALQLYQTIST
                     NFPDISFNIYSGERWYVEREDEWVKQEAAITKMDPEAIAMEDFLNEHFPVHKILCMGN
                     PDDIQKLEDELGGANLSNISYYRSKDTYMEIVNNQVSKLGALHFLCEKYGVALANTLA
                     IGDNFNDMPMIMHAGKGIAMGNAPDEVKQAADCVTDTNDENGFYKALAMSLG"
BASE COUNT          375 a          225 c          266 g          276 t
ORIGIN      
        1 gatatttgtt ttatcggcgc cgcaagtttg gagaaagaag gcatcttttt taaagaaccg
       61 aacaacgcct tgatcaagca gaaagtggta aaacaaagcc ggcaagtggt actggttgct
      121 gaaaatcaga aattcagcaa agaagcgcag tacaaaggcg caaattacca ggacattgat
      181 tgcttcatta cagataggac tctggcatca aaacagcagg cttattttgg agatcaaacc
      241 gagatcatct ttgaggagga aacgatatga caaaggtcga gttggtattc agcgatatag
      301 acggcacgtt gttggattct aagcatgagg tttcacccga agcaaaccaa atcatccagg
      361 acttgatgaa gcaaggcacg aaaattattt tggcatccgc cagaccgcca ggagcaatgg
      421 tttcgattgc cgatgaagtc cgtttgtctt caccactggt ctgcttcaac ggagctctga
      481 tcacccaata taaagaagga tcatttgatg atctgtacag tctgacttta gagcgactgg
      541 acgcgttaca actctatcaa acaatatcca caaattttcc ggatataagc ttcaatatct
      601 attccggcga gcggtggtac gttgaacgag aagatgagtg ggtcaagcag gaagcggcca
      661 tcaccaaaat ggatccggaa gcaatcgcaa tggaagattt cctgaacgag cattttcctg
      721 tccacaagat actgtgcatg ggaaatccgg atgacattca aaaattggag gatgagctgg
      781 gaggggcaaa cttatcaaac atttcctact accgatcaaa agacacctat atggaaatcg
      841 tcaataatca agtatcaaaa ttaggagccc ttcacttttt gtgtgaaaaa tatggcgttg
      901 cattagcaaa taccttagca atcggggaca atttcaacga tatgccgatg atcatgcacg
      961 caggcaaagg aatcgcgatg ggcaacgctc ctgatgaagt aaaacaagca gctgattgtg
     1021 taacggatac gaatgatgag aatggttttt ataaggcttt agcaatgagt ctgggataaa
     1081 tcaaaatggt aaatatagct ttcaaaatcg cctgatacgt cgaaaactat atccctcgga
     1141 aa
//
LOCUS       DAOMUJ010000577         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32665_length_1141_cov_2.132597, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000577 DAOMUJ010000000
VERSION     DAOMUJ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32665_length_1141_cov_2.132597"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..451
                     /locus_tag="PLT73_10585"
     CDS             212..451
                     /locus_tag="PLT73_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRM20856.1"
                     /translation="MIGFIAAILTTLSFVPQAWQIIKTRDTSGISLGMYSMFVTGVFL
                     WLIHGWNIQDYAVIAANGVTLVLASIILAFKIKYK"
     gene            466..>1141
                     /locus_tag="PLT73_10590"
     CDS             466..>1141
                     /locus_tag="PLT73_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM20857.1"
                     /translation="MALDKNFLWGGALAAHQFEGGVLNTSKGLSVADVMTAGGHGVPR
                     VITDGVVEGNYYPNHIGIDFYGRYKEDIALFAEMGFKCFRTSIAWSRIFPNGDEAEPN
                     EEGLQFYDDVFDELLKYGIEPVITLSHFEMPYHLAKEYGGFMNRKTIDFFVNFAEVCF
                     KRYKDKVKYWMTFNEINNQMNYKNNIFGWTNSGAHFGDYPNPEEAMYQCGHYELVASA
                     LAVKIGK"
BASE COUNT          305 a          242 c          292 g          302 t
ORIGIN      
        1 gtttcttctg aacctcacta ttatcctatc atatttgtga atgttatttg ataatccgga
       61 tcaaaaaaac ggaaaatttg taaacggtgt tacggagtgt ccggaaggta ttgtttctgt
      121 tccgctatcc attataatcg agttgtaaaa gtatgtagcc gcctcgttgg tcatttgagt
      181 cgcacattca tagcggtagg gggaataaaa aatgataggt ttcattgcag ccattttgac
      241 tacattgtct tttgttccgc aggcctggca gatcatcaaa acgagggaca cgtccggcat
      301 atccctgggg atgtacagca tgttcgtaac cggggtattc ttgtggctca tccacggctg
      361 gaacatccag gattacgctg tgattgcagc gaacggagtg acattggtgt tggcatcgat
      421 cattttggct tttaaaatca aatacaaata gaaggagaga tagatatggc attggataaa
      481 aattttttgt ggggcggcgc attggctgcc catcagtttg aaggaggggt tttgaacact
      541 tccaaaggct tgagcgtggc ggacgttatg acggcgggtg ggcacggtgt tccgcgcgtt
      601 atcacggacg gtgtggtcga aggcaattat tacccgaacc acatcggcat cgacttctat
      661 ggccgctaca aagaggatat cgctttgttc gcagaaatgg gcttcaagtg cttccgcacc
      721 tcgatcgctt ggtcgcgcat ctttccgaat ggcgatgaag cagagccgaa tgaagaaggc
      781 ctgcagttct acgatgatgt tttcgatgaa ttgttgaaat acgggatcga accggtcatc
      841 acactttccc acttcgagat gccataccac ttggctaagg aatacggcgg tttcatgaac
      901 cgcaagacga tcgatttctt cgtcaacttc gcggaggtct gcttcaagcg ctacaaagac
      961 aaagtgaaat attggatgac cttcaatgaa atcaacaacc aaatgaacta caagaataat
     1021 atcttcggct ggaccaactc aggcgcgcat ttcggcgact atccgaatcc ggaagaagcg
     1081 atgtatcaat gcggacacta tgaattggtc gccagcgcat tggcagtcaa aatcggcaaa
     1141 g
//
LOCUS       DAOMUJ010000578         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32703_length_1140_cov_1.762212, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000578 DAOMUJ010000000
VERSION     DAOMUJ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32703_length_1140_cov_1.762212"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLT73_10595"
     CDS             <1..353
                     /locus_tag="PLT73_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRM20858.1"
                     /translation="TELRWTHVAGNTSDHYKTIRLRIGKGIAGEVWRTGREQRATEIQ
                     SDSQNLLKYPIARLEQLDMTIGIPIVQDGITRAVLLLGYRNPLALNVADMTAVYAALP
                     GLETLLEEEGHKYE"
     gene            346..>1140
                     /locus_tag="PLT73_10600"
     CDS             346..>1140
                     /locus_tag="PLT73_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM20859.1"
                     /translation="MSDSTERISKTLPPPTWIMDADYTIIDRNAQAEKFEKDYVISME
                     YAVEVAKGTCCAFQTDKKACKNCPLENKWAASDGFPITFMGRDGLSYEFFGKLVKTEH
                     IWKLEIRYIDKPFESEGKSMLTYLNEARESEQKRIARELHDGIAQSIYSLMLETRGLK
                     WTPFEEHQQKLQAIDRHFADVLLEIKSLATELRPSILDDVGLIPAINQFVEQTMEMTG
                     FIIHVIVDGERKRLTSRGNVVIYRSIQEAISNALKYSGENEATIALD"
BASE COUNT          330 a          254 c          299 g          257 t
ORIGIN      
        1 aaactgaact gcgttggacg catgttgccg gaaatacaag cgatcactac aaaacgatcc
       61 gcttgcggat cggtaaaggc atcgccggcg aagtttggcg caccggccgg gaacaacgtg
      121 ccactgaaat ccaatcagac agccagaatc ttttgaagta tccaattgca aggctcgagc
      181 agcttgatat gacaatcggc attccgatag tgcaagacgg tatcacacga gctgttttgc
      241 tgctgggata ccgcaaccca ctcgctttga atgtggccga tatgactgct gtctatgcag
      301 ctttgccggg tttggagact ctcttggaag aggaggggca caaatatgag tgactcaact
      361 gagcgcattt ccaaaacttt gcccccaccg acttggatca tggatgccga ctatacaata
      421 atcgacagga atgcgcaggc agaaaagttc gagaaagatt atgtcatttc aatggaatat
      481 gccgtggagg ttgccaaagg cacttgctgt gctttccaaa cggataaaaa agcctgcaaa
      541 aactgtccgc tcgagaacaa atgggctgct tccgacggat ttccgatcac ttttatgggc
      601 cgtgacggat tgtcctatga atttttcggg aaactagtga aaacggaaca tatctggaaa
      661 ctggagattc gctacatcga taagcccttc gaatcagagg ggaaaagcat gctgacatac
      721 ttaaacgagg cacgcgagag tgaacaaaaa aggattgcgc gcgaactcca tgatgggatt
      781 gcccaaagca tctacagctt gatgcttgaa acgagagggc tgaagtggac accgtttgaa
      841 gagcatcagc agaagctcca ggcgattgat cggcattttg cggacgtact attggaaatc
      901 aaaagtttgg ctaccgaact gaggccaagc attctcgatg atgtcgggct tattccggcg
      961 ataaatcagt ttgttgaaca gacaatggaa atgacaggat tcatcatcca tgttatcgtt
     1021 gacggagagc ggaagaggct caccagtcga ggaaatgtcg tcatctaccg cagcatccag
     1081 gaggcgatct cgaatgcttt gaaatattct ggagagaatg aagcgaccat cgcccttgat
//
LOCUS       DAOMUJ010000579         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32819_length_1137_cov_2.471349, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000579 DAOMUJ010000000
VERSION     DAOMUJ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32819_length_1137_cov_2.471349"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1137)
                     /locus_tag="PLT73_10605"
     CDS             complement(<1..>1137)
                     /locus_tag="PLT73_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM20860.1"
                     /translation="IPEDTRLISFLGGKTIIYILSALILIGILIFTMDTISFIFKPLQ
                     IMFSTVVAPVILAIVFYYIFNPLVTFLERRKINRGMATSLVFVLFIFMLVYGFVLLVP
                     ILSNQLTNLVNEFPTYIEQMNAYFDRLLANSTFKDYYEQAQNWLDSTVGSIPEMILAW
                     IGNSSEKIMNIFSTISSVVVVTVTFPVILFFMLKDQGKFKPFVMKNVPPVFRDDVEKI
                     SRQMSLQVGSYVQGQLLVALSLGALLIVGYLIIGLDYAFVLALIATLTAVIPFIGATI
                     GVVPALFVAAFTSPAMLLKMGVVWALAQFIQGNIIEPSIMGRNLKMHPLTIIIVLLIM
                     GKLLGLIGMILGVPLFAMLKILLEHVLEKFKIRYNRYFAEVAGP"
BASE COUNT          342 a          250 c          277 g          268 t
ORIGIN      
        1 aaaggacccg ccacttcagc aaagtaacga ttgtaccgaa tcttgaactt ttcgagtaca
       61 tgctctagca gtattttcag catcgcgaac aatggcacgc ccagaatcat accgattaaa
      121 cccagaagtt tgcccataat caacaaaacg atgatgatcg tcaacggatg cattttcaaa
      181 tttcgaccca tgatgctcgg ttcgatgatg ttgccttgga tgaactgtgc caatgcccag
      241 acgacgccca tttttaggag catggctggt gaagtgaagg ctgcaacgaa caaggctggt
      301 acaacgccaa tagtggcgcc gatgaaaggg atgaccgctg tcaaggtagc gatcagggca
      361 aggacgaagg cataatccaa cccgatgatc aagtacccga ctatcaacaa agcgcccaga
      421 ctcaaggcaa ctagcaactg gccttggaca taggatccga cctgaagcga catctgtctg
      481 gatatttttt cgacatcatc acgaaaaaca ggcgggacgt tcttcatgac aaatggtttg
      541 aacttccctt ggtccttcag catgaaaaaa aggatgaccg ggaaagtgac agtcacgacc
      601 actacgctcg aaattgtgga aaagatgttc ataatttttt cgcttgaatt gccgatccag
      661 gccagaatca tttcagggat gctgcccacg gtgctgtcca accaattttg cgcttgttca
      721 taataatcct tgaaggtgct gtttgcgagc agtctgtcga aataggcatt catttgttcg
      781 atataggtag gaaattcatt gaccagattc gtcagctgat tcgataaaat cggaaccaaa
      841 agcacaaatc cgtagaccaa cataaaaatg aagaggacga agacgagcga tgtcgccatt
      901 ccccggttga ttttccttct ttcgaggaag gtcaccagcg gattgaagat ataatagaag
      961 acgattgcta aaatcacggg tgctacgact gttgaaaaca tgatctgcag cggtttgaag
     1021 atgaaggaga tggtatccat cgtgaagatg agtatgccga tcagaatcag ggcggacagg
     1081 atatagatga ttgtcttgcc gcccaaaaag gagatgaggc gcgtatcttc gggtatt
//
LOCUS       DAOMUJ010000580         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_32864_length_1136_cov_1.773358, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000580 DAOMUJ010000000
VERSION     DAOMUJ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32864_length_1136_cov_1.773358"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLT73_10610"
     CDS             complement(<1..435)
                     /locus_tag="PLT73_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRM20861.1"
                     /translation="MNRVVITGMGAITPLGNTVTEFWDGIKNGKNGIAAITNFDASET
                     GISVAGEVKDFDATKYMDRKEYKRMDLFSQYAVAASAQAVEQSGIDMEKIDADRFGVT
                     IGSGVGGFITMESGIIKMHEKGPKRVPPMFVPMAIGNMAGGNT"
     gene            complement(451..1136)
                     /gene="fabG"
                     /locus_tag="PLT73_10615"
                     /pseudo
     CDS             complement(451..1136)
                     /gene="fabG"
                     /locus_tag="PLT73_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030386.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
BASE COUNT          260 a          287 c          246 g          343 t
ORIGIN      
        1 agtatttcct cccgccatgt tgccgattgc catcggaacg aacataggtg ggacgcgttt
       61 cggtcctttt tcatgcattt tgatgatgcc ggattccatt gtaatgaaac cgccgacacc
      121 agagccgatg gtaacgccaa agcggtccgc atcgattttt tccatgtcga tgccgctttg
      181 ttccacggct tgagcagagg cagctaccgc atattgcgaa aacaaatcca tacgcttgta
      241 ttcttttcgg tccatatact tggtcgcatc aaagtctttc acttcacctg cgacggatat
      301 gcccgtttcg cttgcatcaa aatttgtgat agcagcaata ccgtttttac cgtttttgat
      361 gccgtcccaa aattcagtga ctgtatttcc taaaggggtg atagcaccca tacctgtaat
      421 aacaacgcga ttcatattta tcctcctgtc ttacatgtac atgccgccat tgacactgat
      481 ggtctggccg gtgatgtaag tattctcact caagaagaca gcagtgtttg ccacttcttc
      541 ggcttttcca aatcgtttca atgggatttg cgtcaacatt tgttcttgaa tcttttccga
      601 aagaacatcc gtcatttccg tttcgatgaa tcctggtgca atggcattac acgtaatgcc
      661 tcttgtcgca gcttcacggg cgacagattt cgtcaatccg atgataccgg ctttgctggc
      721 ggcatagttg gcctgccctg tatttccggt ttcaccgact acgctggaaa tattgatgat
      781 gctgccggct ctttgtttca gcattagagg caaggcgtgg cggatcatat tgaaggtacc
      841 tgataagtgg catcaaagtc ctcctcatcc atgcgcatga tcaatttgtc gcgggtaatc
      901 ccagcgttgt tgaccaaaac atcaacagaa ccgaacagtt ctttggtctg gctgacgatt
      961 tctttggcag tctcaaaatc gctgacatct cctaggagcg tttttacagt tacaccgtaa
     1021 gattccagat gcgcaacttt ttcttcagca atcggtttac gtgcattcag aacgatattg
     1081 gctccacgtt tcgcataagc ttcagcgatg gcttcgccga ttcctcttga actacc
//
LOCUS       DAOMUJ010000581         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_33373_length_1121_cov_2.141651, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000581 DAOMUJ010000000
VERSION     DAOMUJ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33373_length_1121_cov_2.141651"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..910
                     /locus_tag="PLT73_10620"
                     /pseudo
     CDS             1..910
                     /locus_tag="PLT73_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            928..>1121
                     /locus_tag="PLT73_10625"
     CDS             928..>1121
                     /locus_tag="PLT73_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM20862.1"
                     /translation="MTSKRVTVIGAGLAGSEAAFQIAEQGIQVDLYEMRPKKMTPAHH
                     TSGFAELVCTNSLRANQVTNA"
BASE COUNT          344 a          245 c          288 g          244 t
ORIGIN      
        1 actccggcaa tcttcgatac gatgaaggta gatctcaagc atgaggatgt tatgttccgc
       61 gcgacaggtt ccaaggagaa ctttgccggt taccgcaaag tctacgtaga aggcaattcc
      121 aaagaaaaag ataaccttct tccggaaatg gctgtcggcg atacggtttt ctcaaaagac
      181 atcgacccga atcagcattt ctcacaaccg ccggcccgat actcggaagc aacgctcatc
      241 aaagctttgg aggagaacgg tgtcggtaga ccttcgacct atgcgccgac aatcgaaacg
      301 atccagaagc gttattacgt aaaagtgaca gcaaaacgtt tcgaaccaac cgaattgggt
      361 aaacggtttg atttccactt atttcccgga tatcgtgaat atttccttca ctgccgggat
      421 ggaacaagat ctagacagtg tcgaagaggg aaaaatggaa tgggttaaag tagtcgatcg
      481 tttttataag ccattcgccg aagagctagc gaaagcggaa gtcgagatcg aaaaaattga
      541 gatcaaggac gagcctgccg gattcgattg cgaattatgc ggccatccga tggtcatcaa
      601 aatcggcaga ttcggaaaat tctatgcatg cagcaatttc ccggaatgtc gcaacacgaa
      661 gccgatcgtc aagaaaatca acgtgacgtg tcctgtgtgc aaaaaaggcg aggtcatcga
      721 gaggaaatcc aagaaaaatc ggatattcta tgggtgtgat cgttacccag agtgcgaatt
      781 catctcctgg gacaaaccga ttggccgtga ttgcccgaaa tgtcaacagt atcttgttga
      841 gaagaaggtc aaaggcggca tccaagtgaa atgcagtcat tgtgaatacg aggaagacat
      901 acagaaataa cgttatggag gcatactatg acaagtaaaa gagttaccgt gatcggagcc
      961 ggcttggccg gaagcgaagc ggcttttcaa atagcggagc agggtatcca agtcgatctc
     1021 tatgagatga ggccgaaaaa gatgacgcct gcccaccata cttcaggctt cgcggaattg
     1081 gtctgtacca attcactgcg ggcgaatcaa gtcacgaacg c
//
LOCUS       DAOMUJ010000582         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_33461_length_1119_cov_2.295113, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000582 DAOMUJ010000000
VERSION     DAOMUJ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33461_length_1119_cov_2.295113"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..651
                     /locus_tag="PLT73_10630"
     CDS             106..651
                     /locus_tag="PLT73_10630"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRM20863.1"
                     /translation="MKEYIRKEMLGKLRDLPAQDRIRMEKELTKAVCSSQEWKASKTI
                     GVTWSNFPEIDTHTIIKNGLEEGKRMVVPYSGKNRVMTFHEYSPDIAMAKSKFGIMEP
                     VDKSNPVPPGEIDLLLVPGLAFSETGFRIGFGGGYYDRYLAAYNGHTLSVLFPFQLFK
                     NPHWEIEAFDVPIQKLLLATI"
     gene            684..>1119
                     /locus_tag="PLT73_10635"
     CDS             684..>1119
                     /locus_tag="PLT73_10635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRM20864.1"
                     /translation="MMRSYNTRKSYLPDKFSVTHLFLAIQIVIFLLMTLNGGSTNVVT
                     LILFGAKFNPAIAQGEWWRLIAPMFIHIGFTHILVNSITLYYLGTQMESLYGSLRFAL
                     IYLLSGLMGNLMSFAFNDSISAGASPSLFGLFAAAIVLGRQFP"
BASE COUNT          295 a          229 c          267 g          328 t
ORIGIN      
        1 aattaaaatg acaacaggat atcctgttgt ttttttattt tttccggaag gcttatttta
       61 agcagaaata gtgtgcgaca gattgttttt tgaggaggtt attggatgaa agaatatata
      121 agaaaagaaa tgcttgggaa actgagggat ttgcccgccc aggatcgcat tcggatggaa
      181 aaagaattga caaaagcagt ctgctccagt caagagtgga aagcttcaaa gacgataggc
      241 gtcacatggt ccaattttcc ggaaatcgat acacatacaa tcattaaaaa cggacttgaa
      301 gaaggcaaac ggatggttgt tccctattcc ggcaagaatc gtgtgatgac ttttcatgaa
      361 tactcgccgg atatcgcgat ggcaaaaagc aaattcggta tcatggaacc tgtcgataag
      421 agcaatccgg ttccgcctgg agaaatcgat ctgctgctcg ttcctggatt ggcattttca
      481 gaaacaggtt tccgtatcgg gttcggcggc ggttattatg accgctatct ggctgcgtac
      541 aacggacata ccttgtcggt gttgttccca ttccaattgt ttaagaatcc gcattgggaa
      601 attgaagctt ttgatgtgcc gatccaaaag ttgttgctgg caacaatcta gtttaaaata
      661 ggatcataat gaaaggacgt gtcatgatgc ggtcatataa taccagaaaa tcctatttgc
      721 cggataaatt ctcagtaaca catttgttct tggccattca gattgttatc ttcctgctga
      781 tgaccttgaa cgggggaagc accaatgtag tgacgcttat tcttttcggc gccaaattca
      841 atccagccat cgcgcaaggg gagtggtggc gtttgatagc acctatgttc atccacatag
      901 gcttcacgca cattctggtc aacagtatca ccctgtatta cttaggtaca cagatggaaa
      961 gtctctatgg ctctttgcgt tttgcgctga tttatctcct gagcggcttg atggggaatc
     1021 tgatgagctt cgccttcaat gattcgattt ccgctggtgc aagcccctcg cttttcgggt
     1081 tgttcgctgc agccattgtt ctgggccgac agtttcctt
//
LOCUS       DAOMUJ010000583         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_33505_length_1118_cov_1.980245, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000583 DAOMUJ010000000
VERSION     DAOMUJ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33505_length_1118_cov_1.980245"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLT73_10640"
     CDS             complement(<1..924)
                     /locus_tag="PLT73_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM20865.1"
                     /translation="MALREDFLWGGATAANQCEGGYNEGGRGLANVDVVPVGKDRGAV
                     ITGKMKMFDFDDEHFYPALEAIDMYHRYKEDIALFGEMGFKTYRLSIAWTRIFPNGDE
                     TEPNEEGLQFYEDLFKECHKYGIEPLVTITHFDCPMHLIKEYGGWRNRKMVGFYENLC
                     HAIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKFQSAHHELMASAI
                     ATRIAHEVDPENQVGCMLAAGKYYPYTCDPKDVFKAQEEDRENYFFIDVQSRGEYPAY
                     ALKKLEREGIKIEMEEGDKEILKENTVDFISF"
BASE COUNT          268 a          271 c          236 g          343 t
ORIGIN      
        1 gaaggaaatg aaatcgacag tattttcctt caggatttct ttgtcgcctt cctccatctc
       61 gattttgatg ccttcgcgct ccaatttttt cagcgcgtaa gcagggtatt ccccgcgtga
      121 ttgcacgtcg atgaagaagt agttttcgcg gtcttcttct tgcgctttga agacgtcttt
      181 agggtcgcaa gtatatggat agtatttccc ggcagccaac atacagccca cttggttttc
      241 aggatcgact tcatgcgcga tgcgggtagc gattgcgctg gccatcaact cgtgatgcgc
      301 agattggaat ttgacttgtt cttcgttttc gccttcttcg aagtacagac cagcacccat
      361 gaatggcgcg tgcagaatca tgttgatttc attaaaagtc aaccaatatt taaccagacc
      421 tttgtagcgg ttgaagatgg cgtggcacag attttcatag aagccgacca tcttacggtt
      481 gcgccagcca ccgtattctt tgatcaagtg catcgggcaa tcgaaatgcg tgattgtcac
      541 taatggctcg attccgtatt tatggcactc tttgaacagg tcttcgtaga attgcagccc
      601 ttcttcattc ggctccgttt cgtctccatt tgggaagatg cgtgtccatg cgatcgataa
      661 acggtaagtt ttgaagccca tttcgccgaa taatgcgatg tcttctttat agcggtgata
      721 catgtcgata gcttccaacg caggatagaa atgctcatca tcaaaatcga acattttcat
      781 tttccctgtg atgacggccc ctctgtcctt gccgacggga acaacgtcaa cgttcgccaa
      841 accgcgtcca ccttcgttgt atccaccttc acactgattg gcagcagtag cgccgcccca
      901 taaaaagtct tctcttaaag ccattgtcca tcctcctaga tctttttata ctaaaatgtt
      961 gcatacagtt tcattgcttt cgctcatgca aaagctttca gcaaccgctg ccattcattt
     1021 gaatcttgcc ggtatcggtt acattattaa tactaacact tttggatgga caatgtaaat
     1081 attgttagac aaggaatcaa attattgtgt actcacag
//
LOCUS       DAOMUJ010000584         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_33765_length_1111_cov_1.660985, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000584 DAOMUJ010000000
VERSION     DAOMUJ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33765_length_1111_cov_1.660985"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..>1111
                     /locus_tag="PLT73_10645"
     CDS             161..>1111
                     /locus_tag="PLT73_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20866.1"
                     /translation="MMKITKKKLDDMYRSYCASYITIGGKTCALIASEEEGYPCYMYS
                     GKDFEKKETVWEKGGGCMSIIPIPNKENEFLAIVDFYLKVSPSKAKVVWGKYDSETGW
                     EIKDVLYLPYLHRFDIYDVDGVNYFVGATIADDKDFKDDWSRSGSIYYGVLPEDPTQG
                     IELNVLGKGYFRNHGYYKDYEGNKPVGYFTSDQGIIKVTPFADKEWEIEQILDSPIGE
                     VALFDIDSDGEKEMITIEPFHGDSVKIYKKTESGYEAVYEYPHKIDFAHTLVGTTLRG
                     VPSFVGGIRRENCDLFYIQYVDGEFKTEIVDKDCGPSNIMV"
BASE COUNT          327 a          240 c          294 g          250 t
ORIGIN      
        1 acggcatcga cacttacggt gcaagcggca acgggaacac ggtgatggct gagttcggct
       61 ttacgaccga aaaagtggtt gcggcttatc aagcgaagtt cgtaaagtaa atggaatcct
      121 gctgcaacag tgcggggtaa atgatgaagg gagtacatta atgatgaaaa taaccaagaa
      181 gaaattggat gatatgtacc gcagttattg cgccagctac atcacgatcg gcggcaaaac
      241 gtgcgcgttg atcgccagcg aagaggaagg ctacccttgc tacatgtatt caggcaagga
      301 tttcgaaaaa aaagaaaccg tttgggaaaa gggcggtggc tgcatgtcca tcatccccat
      361 tccgaataaa gaaaatgaat tcttggccat cgtcgatttt tacctgaagg tcagcccaag
      421 caaagcgaaa gttgtctggg gtaagtatga ttccgaaaca ggttgggaaa tcaaggacgt
      481 gctgtacttg ccatatctgc accgtttcga tatctatgac gtggatggcg tgaattactt
      541 tgtcggcgcc acgatagcgg atgacaagga cttcaaagat gactggtcgc gatcaggcag
      601 catctattat ggcgttctgc cggaggaccc tacgcaaggc atcgaactga atgttctggg
      661 gaaaggctat ttccgcaacc atggctacta caaggattac gagggcaaca aaccagtcgg
      721 ctacttcaca agtgatcaag ggatcatcaa agtgacgcct tttgctgata aagaatggga
      781 aatcgagcag attttagaca gccctatcgg cgaagttgca ctgtttgaca tcgactcgga
      841 cggggaaaaa gaaatgatca cgatcgaacc tttccacggt gacagcgtca aaatctacaa
      901 aaagacggaa tccggttatg aagcggtcta cgaatatccg cataaaatcg atttcgccca
      961 tacattggtg ggtacgacgc ttagaggcgt ccctagcttt gtcggcggca tccgtcgcga
     1021 aaactgcgac ctgttctata ttcaatatgt ggacggtgaa ttcaaaaccg aaatcgtcga
     1081 caaagactgc gggccttcca atatcatggt g
//
LOCUS       DAOMUJ010000585         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_33955_length_1106_cov_1.768792, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000585 DAOMUJ010000000
VERSION     DAOMUJ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33955_length_1106_cov_1.768792"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="pepT"
                     /locus_tag="PLT73_10650"
     CDS             <1..879
                     /gene="pepT"
                     /locus_tag="PLT73_10650"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM20867.1"
                     /translation="ALKNYIGQTLITTDGKTLLGADDKAGIAEIITALEAIKTADDIE
                     HGDIKVAFGPDEEIGRGADLFDVAGFGCDFAYTMDGGPLGELEYESFNAAQAIVMIRG
                     KNVHPGTAKDTMVNAIKLAIAYDGALPQNEVPEKTEKREGFYHLLGIEGNVEESKMTY
                     IIRDHDKELFENKKTTMLALAERMNKELTEDRISIEMHDQYYNMGEVLKNDMRPVDLA
                     ETAMKALGINPIIEPIRGGTDGSKLSFMGLPTPNIFAGGENFHGRYEFVSVQSMEKAV
                     AVIVEIIRQSQGYGKQ"
     gene            876..>1106
                     /locus_tag="PLT73_10655"
     CDS             876..>1106
                     /locus_tag="PLT73_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRM20868.1"
                     /translation="MRNTVLTYLVVTNVLLFILMGVDKKKARQKAWRIPERNLLLLGF
                     LGGGLGGFLGMHHFRHKTKHLIFKVIFGLGTLL"
BASE COUNT          320 a          239 c          298 g          249 t
ORIGIN      
        1 gcgttgaaga actacatcgg tcagacattg atcactaccg atggaaaaac gttgttgggt
       61 gctgacgaca aagccggcat cgctgaaatc atcacagcct tggaagcgat aaaaacagcg
      121 gatgacatag agcacgggga catcaaagtc gccttcgggc ccgatgaaga aattggccgc
      181 ggagccgatc tttttgatgt ggccggtttc ggttgcgact ttgcctacac gatggatggc
      241 ggcccgttgg gtgaacttga atacgaaagt ttcaatgcgg cgcaagccat cgtgatgatc
      301 cgtggcaaaa acgtgcatcc aggcactgcc aaggacacaa tggtgaatgc gatcaaatta
      361 gctatcgcat acgacggcgc tttgccacaa aatgaagtcc ctgaaaagac tgaaaagcgt
      421 gaaggcttct atcatctgtt gggcattgaa ggcaatgtgg aagaaagcaa gatgacctac
      481 atcatcaggg accatgacaa ggagcttttt gagaacaaga aaacaaccat gcttgcgctg
      541 gcagagcgca tgaacaagga attgaccgag gaccgcattt ctatcgaaat gcacgatcaa
      601 tactacaaca tgggtgaagt gctgaagaac gatatgcgtc cggtcgattt ggcggaaaca
      661 gcgatgaagg cattggggat caatccgatc atcgaaccga tccgcggcgg gactgacggc
      721 tcaaaattat ccttcatggg tttgccgacc ccgaatattt ttgccggggg agaaaacttc
      781 catgggcgct atgaatttgt gtctgtccaa tcaatggaaa aagcagtagc tgtcatcgtg
      841 gaaatcatca gacaaagtca aggctatgga aagcaatgag aaacaccgtt ctgacgtatc
      901 tcgttgtgac gaatgttttg ctctttatat tgatgggcgt agacaaaaag aaagcgcggc
      961 aaaaagcttg gcgcataccg gaacgcaatc tacttttgct cggatttcta gggggcgggt
     1021 taggcggatt cctggggatg catcactttc gccataaaac aaaacacctg attttcaagg
     1081 taatcttcgg tttgggtaca ctcttg
//
LOCUS       DAOMUJ010000586         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34055_length_1103_cov_2.465649, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000586 DAOMUJ010000000
VERSION     DAOMUJ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34055_length_1103_cov_2.465649"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLT73_10660"
     CDS             complement(<1..232)
                     /locus_tag="PLT73_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20869.1"
                     /translation="MKFSIKVVSLFIVFIFIAYALPLYLDNDERIAPTSSIARNEQTA
                     IDENEGYPLPVTGYESYIGQSITAYTAKHGQPN"
     gene            complement(395..>1103)
                     /locus_tag="PLT73_10665"
     CDS             complement(395..>1103)
                     /locus_tag="PLT73_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM20870.1"
                     /translation="LLITAAAVMMVFLQPDLGGAAIIAFICLVMFLHCGIKVKYGIMT
                     FGAIGAIYGLIIVVVRTFGSSIPFFHSYQIERITSFIDPFADIQDSGYQVVNSYYALS
                     RGGLFGVGIGESIQKSGYLPEPHTDFILSIVGEELGLVGVAFVLVLFFYMVYRIFKNA
                     IKIKDPFAQLVCTGIGTMFLIQGVINVGGATGLMPLTGVTFPFVSYGGSSLLVSAVSI
                     GLVNNMYINDQISKQPK"
BASE COUNT          322 a          266 c          241 g          274 t
ORIGIN      
        1 gattcggctg cccgtgcttc gctgtgtagg ctgtaataga ttgaccgatg taggattcat
       61 agccggtaac cggcaaagga tacccttcat tttcatcaat ggccgtttgc tcgttcctcg
      121 cgatgcttga agttggcgca atacgttcat cattgtccaa ataaagaggg agtgcataag
      181 ctatgaatat aaatacaatg aacaatgaca ctactttgat ggaaaatttc acgcctaccc
      241 ctccttgtga attctgattg ttgaatatgg taaagaatac catggtacct actattatac
      301 tctattttcg tcagatacaa aaagcattcc ggacgtttat ccaccggggc aaccactcaa
      361 ataaaaagag ccgaaaatcg gctcttttta ttgtttattt tggttgtttg gaaatctgat
      421 cattgatgta catattgttg accagtccaa tcgaaacggc cgataccaac aagctggatc
      481 cgccataact gacgaacgga aaagtgactc cagtcaacgg catcaagccg gtcgcaccac
      541 cgacattgat gactccctgt atcaggaaca tcgtaccgat cccggtacag accagttggg
      601 caaaaggatc tttgattttg atcgcattct taaaaatgcg atagaccata tagaaaaaca
      661 gcaccaaaac gaacgcaact cccaccaagc ctagctcttc ccctacaatg gacaagatga
      721 aatcggtgtg tggttccggc agatagcccg atttctggat gctttcaccg atgcctacac
      781 cgaaaaggcc gcccctactc aaagcgtagt aagaattcac tacttgataa ccggaatcct
      841 gtatgtcggc aaaaggatcg atgaatgatg tgatcctttc tatctgatag gaatggaaga
      901 aagggatgct gcttccgaat gtcctcacca cgacgatgat cagtccgtat atcgctccga
      961 tagccccgaa agtcatgatg ccatatttga ctttgatgcc gcagtggagg aacatgacca
     1021 gacagataaa ggcgatgatg gcagcacccc caagatcggg ctgcaggaac accatcatca
     1081 ctgctgccgc cgttatcagg agc
//
LOCUS       DAOMUJ010000587         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34336_length_1096_cov_3.078770, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000587 DAOMUJ010000000
VERSION     DAOMUJ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34336_length_1096_cov_3.078770"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLT73_10670"
     CDS             complement(<1..358)
                     /locus_tag="PLT73_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM20871.1"
                     /translation="MISMAGKAIRKWWALFALPTFAAFIIGFVIPFIMGVYLSFCKFT
                     TVTDSEFIGLKNYQKALADKEFLHALGFSTALTIVTTVVINVVAFAIAYMLTKAIKGS
                     NIFRSVFFMPNLIGGII"
     gene            701..>1096
                     /locus_tag="PLT73_10675"
     CDS             701..>1096
                     /locus_tag="PLT73_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20872.1"
                     /translation="MKSSIQDVAQLAHVSISTVSRSFTRPDLVSKATRDKVMKAADEL
                     NFSISRSAAALKTGRALRIAVLISGRLNLWFSSSIMEGLNESFHDEGYDISIYQMSSA
                     EERRAFFDMLPVRRNVDAVIVISFDIASNE"
BASE COUNT          299 a          294 c          233 g          270 t
ORIGIN      
        1 ggataatgcc accgatgagg ttcggcatga agaacaccga acggaagatg ttggatccct
       61 tgatggcctt ggtaagcata tatgcgatgg caaacgcaac cacattgatc acaaccgtag
      121 tgacaatggt cagcgccgtc gagaaaccta acgcatgcag gaattcctta tccgcaagtg
      181 ccttctgata gtttttaagt ccaatgaatt ccgagtcagt cacagttgtg aacttgcaga
      241 aactcaaata cacacccatg atgaacggga ttacaaaccc gatgataaat gcagcgaacg
      301 tcggtagcgc aaacagagcc caccactttc gtatcgcttt gccggccatg ctgatcatag
      361 cttgcctgct ttctttcctc tccattccaa caccgcagca acgcggcatg gaaaaccaac
      421 tgccccaaat cggcgaatca ctgcattgtt tttcgccttt ttagttttta cagttgttat
      481 aataaacagc gcgacgcgaa atgtcaaacg ttgtcatttt tacatgtcat aaatcatata
      541 ttctgcatcg ttgtcatagt attgacagcc tgaaagcatt gtaaatatca gccccgcaac
      601 aactgtggca caaatgaaaa cgtttgtaat ccaacttttg caagagtgtt tcaatacagt
      661 atgctgaata tccagtagtt ctactataca aggggtcacc atgaaaagca gcattcaaga
      721 cgtggcacaa cttgcgcacg tctccatttc aacggtttct cgctccttca cacgtcccga
      781 ccttgtgtcg aaagccacgc gtgacaaagt catgaaagcc gccgacgaat taaatttttc
      841 catctcgcgc tccgctgcag cactcaaaac cggccgcgcg ctacgaatcg cggtgctgat
      901 ttccggccga ctcaatctgt ggttcagttc gtccatcatg gaaggactga acgaatcctt
      961 ccatgatgaa ggctacgaca tctcgatcta tcagatgtcc agcgccgagg aacgccgcgc
     1021 gttcttcgac atgctgccgg tacgccgcaa tgtcgacgcc gtaatcgtca tttccttcga
     1081 catcgccagc aacgaa
//
LOCUS       DAOMUJ010000588         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34411_length_1094_cov_2.529355, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000588 DAOMUJ010000000
VERSION     DAOMUJ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34411_length_1094_cov_2.529355"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLT73_10680"
     CDS             complement(<1..791)
                     /locus_tag="PLT73_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRM20873.1"
                     /translation="MNALRKYIKNPKIRQIMVLFQQHWGRMEIGRQSAEMAYYVLLAL
                     LPTLLLLGNIIPLLPLPREQVLSYVEMALPLEVSNTLTPILEQYLNSGSSGVVSFGLV
                     LSIWTASKAFNVFQNVLNQVYNTKMRKNFIVHRIFSFVIAFLLVAMIAFVAFLFMFGR
                     YIRDFFEEFLSIQLDFITAFENFRWIVAFLIIAIIMTVIYYVVPNVRWSFKYSLPGAA
                     FATIGFLLISQLFSLYVGVAGNQAIGNSAIGVVITLLLWLYLMGTV"
BASE COUNT          292 a          291 c          268 g          243 t
ORIGIN      
        1 accgttccca tcagatacaa ccacaacagc aatgtgatca cgaccccgat cgcactgtta
       61 ccgatggcct gattgccggc gacaccgaca tacaaggaga acagctgcga aatcaagagg
      121 aagccgatcg ttgcgaacgc tgcacccggt agcgaatatt taaatgacca gcgcacattc
      181 ggcaccacgt aataaatgac tgtcatgata atagcgatga tcaaaaaggc aacgatccag
      241 cggaaatttt cgaaggctgt aatgaagtcc aactggatgg acaaaaactc ttcgaagaaa
      301 tcccggatat acctgccgaa catgaacagg aaagcgacaa acgcaatcat ggcgacaagc
      361 agaaaggcga tcacgaacga aaagattctg tggacgatga aatttttccg catcttcgtg
      421 ttgtacactt gattcaaaac gttctggaag acattgaatg ctttcgacgc cgtccagatc
      481 gacagcacta gtccgaatga aacgacccca ctgctgccac tgttcaagta ttgttccagt
      541 atcggggtga gtgtattgct gacttcaagc ggtagggcca tctccacgta ggagagtacc
      601 tgctcccgcg gcaacggtaa taacgggatg atgttcccca gcaacaacag tgtaggaagc
      661 agcgccaaca aaacatagta cgccatctca gccgactgtc gcccgatttc catccggccc
      721 caatgttgct ggaaaaggac catgatttgg cgaatttttg gattcttgat gtattttctt
      781 aatgcgttca tttgcttttt cctcccgcca agatctatag cttaatcata gacaaaagtg
      841 agatcgattg ccacagatat gcattgtgcg tgcttattcc agtgatctga cctggtccgg
      901 ggaaggtagt tctcgccgga gatgaagatc ccggtaaact gtcacctccg gcttgcgggg
      961 cttcgccgaa gttcgccctc cggataacgc ctgtcctccg gccaacattc gctcgccgga
     1021 gttggtcgcc ggcacgctcg ctgttctccg aggaacggcc cgctcaccgg cgctagcgtt
     1081 ttccgccggc gaag
//
LOCUS       DAOMUJ010000589         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34461_length_1093_cov_1.025048, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000589 DAOMUJ010000000
VERSION     DAOMUJ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34461_length_1093_cov_1.025048"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..503
                     /locus_tag="PLT73_10685"
     CDS             219..503
                     /locus_tag="PLT73_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM20874.1"
                     /translation="MYNPQLDTFLVVAETGSFSKASEKLFISPTAVSKQINHLESKIG
                     LTLFNRTPRGMTLTAAGESMYRDSLYLIQYAKDSISRAENAEDGMTAPYG"
     gene            515..>1093
                     /locus_tag="PLT73_10690"
     CDS             515..>1093
                     /locus_tag="PLT73_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM20875.1"
                     /translation="MTSSQSIAEIWSMLRLSEPNFKFQLVSFENTPENAREILRNLGE
                     NIDIVAGVFDENFLQSRKCSTMQLSREPICVALSIYHPLAKKDSLEISDLYGENLMII
                     RKGWNTYVDKLRDDLWNHHPRITIKDFSFYDIEIFNRCASSDELLMAIGKWNNVHPLV
                     KVIPVSWNHTIPFGLLHSPKPTVKVQKLLSAIN"
BASE COUNT          331 a          226 c          239 g          297 t
ORIGIN      
        1 gctgatgctg ttttttctgg actatgttgc gatcatgggg ttgttcatct gtatcgggca
       61 ttatgcagag gtgttgttga gaggcagaaa aaggaaggca agaagctgag aagcgctcct
      121 ttcttttttg tatttcgata agcaaaattg gtataatcat ggctatatag cctgaccata
      181 atagggacag atttgaatgc agtgaggcaa ggactagtat gtataatcca caacttgata
      241 cttttcttgt cgttgcggaa acaggcagct tttcaaaagc atccgaaaaa ttgttcattt
      301 ccccaacggc agtaagcaaa cagatcaatc atctggaaag caaaatcgga ctaaccttgt
      361 tcaaccgaac gcccagaggc atgaccttga ctgctgccgg ggaatcgatg tacagagact
      421 ccctttatct tattcaatac gccaaagatt ccatatccag ggccgaaaat gctgaggacg
      481 gaatgactgc accatacgga taggaacgtc ggttatgacc tcctcccagt ccattgcgga
      541 gatatggtca atgctcaggc tttctgagcc gaacttcaaa tttcagttgg tatcttttga
      601 aaacacgccg gagaatgctc gtgaaatttt acgaaatttg ggagaaaaca ttgatattgt
      661 tgctggtgtg tttgatgaaa attttttgca aagtcgaaaa tgctcaacaa tgcaactctc
      721 aagagaaccc atttgtgtcg cattatcgat ctatcatcct ttagccaaga aggacagcct
      781 tgaaatcagc gatctatatg gagaaaacct gatgattatc cgaaaaggat ggaatactta
      841 tgtcgacaaa cttagggatg atctgtggaa ccatcatccg cgcatcacga taaaagactt
      901 ctccttttat gacatagaaa tattcaatcg atgcgcatct tcagatgagt tgctgatggc
      961 aatcggaaaa tggaacaatg ttcacccgtt agtaaaagtc atacccgttt cgtggaacca
     1021 cacaattcct tttggactgc tgcactcccc aaagccaaca gtaaaagtgc aaaaattgtt
     1081 gtccgccatc aac
//
LOCUS       DAOMUJ010000590         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34602_length_1089_cov_1.988395, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000590 DAOMUJ010000000
VERSION     DAOMUJ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34602_length_1089_cov_1.988395"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLT73_10695"
     CDS             <1..324
                     /locus_tag="PLT73_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRM20876.1"
                     /translation="VIFSGYPVNTTQYVEDDSSFFASLIQFLFILLLLSFFFGGRGGR
                     GGRGGGMNPLWWLLLSGGGGSSYRGNNHRGGYGGGGFGGGGFGGGGGFGGGGSSGGGG
                     SSRGF"
     gene            complement(397..>1089)
                     /locus_tag="PLT73_10700"
     CDS             complement(397..>1089)
                     /locus_tag="PLT73_10700"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRM20877.1"
                     /translation="MKDLEPVLEEAKELKAFVIHTLVVENLSEFTDDFCEAENLPCYN
                     ILKDLVKDISKATGQLPLKRAGALHRLDDEYFNRISAIEFAVKYDDGKDPKGFLDADL
                     VLLGVSRTSKTPLSMFLANKNIKVANLPIMPEASVPEEIWQVDPKKIVGLTNDAEVLN
                     NFRKERMIAYGLSPETIYSDMDRIRAELEYAYDLYNKLGCIVINVSKRSIEETAAIIM
                     SSIEISDTTIIP"
BASE COUNT          240 a          243 c          284 g          322 t
ORIGIN      
        1 gtgattttct ccggttatcc tgtcaatacg acgcaatatg tggaggacga ttcgtcgttc
       61 ttcgcatccc ttatccaatt cctgtttatt cttttgctgt tgtccttctt ttttggtggc
      121 agaggcggtc gcggtggacg tggtggtgga atgaacccgt tgtggtggtt gcttcttagt
      181 ggaggcggcg ggagcagtta ccgcggtaac aatcatcgtg gcggatatgg cggaggcgga
      241 tttggaggcg gtggatttgg tggtggtggc ggcttcggag gcggcggttc ttccggaggc
      301 ggtggatcca gcagagggtt ctgaccaaac tgcccggctg caaaatagaa aaaagaagag
      361 ggcccgaatg aatcaacggg ttctcttctt ttggttttac ggaatgatgg ttgtatcgga
      421 gatttcgatg ctgctcatga tgatcgcggc tgtttcttct atagaacgtt tggatacatt
      481 gatgacaatg caacccaatt tgttgtacaa atcatacgca tattccaatt cagcccggat
      541 acgatccatg tccgaataga tggtttcagg cgacaatccg taggcgatca tccgttcttt
      601 tcggaaatta ttcaacactt cggcatcatt ggtcaatccg acgattttct tcggatccac
      661 ttgccaaatc tcttcgggca ctgaggcttc cggcataatc ggcaagtttg ctactttgat
      721 gtttttgttt gccaaaaaca tggaaagtgg ggtttttgaa gtgcgcgaaa cgcccagcag
      781 gacgagatcc gcatccaaaa agccttttgg atcttttccg tcatcatatt tgacagcgaa
      841 ttcgatagcg ctgatccggt tgaaatattc gtcgtccaga cggtgcaaag cgcccgccct
      901 cttgagtggc agttgtcccg ttgctttgga aatatccttg accaagtcct tcagtatgtt
      961 atagcaaggc aaattctctg cttcgcagaa atcgtctgtg aactcactca aattttcgac
     1021 gaccaaagta tggatgacaa atgctttcag ctctttggcc tcttccaata ctggctccag
     1081 atccttcat
//
LOCUS       DAOMUJ010000591         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34669_length_1088_cov_1.374637, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000591 DAOMUJ010000000
VERSION     DAOMUJ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34669_length_1088_cov_1.374637"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>444
                     /locus_tag="PLT73_10705"
     CDS             <1..>444
                     /locus_tag="PLT73_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRM20878.1"
                     /translation="VAASTTISQMESDIIGEVGNISMSQAATTLSEILGHKVHITTPQ
                     VFVKSIREVIDAAVKPKIITSIGFKKGLTGNNLLLMDAQDSVIIANLMMGGDGQVTSS
                     ELTELELSAVGEAMNQMMGSSATAMATMLGKMIDILPPEVHLVDD"
     assembly_gap    445..464
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <465..1016
                     /gene="fliN"
                     /locus_tag="PLT73_10710"
     CDS             <465..1016
                     /gene="fliN"
                     /locus_tag="PLT73_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRM20879.1"
                     /translation="EHFNVDIGVDNLNADVCLIAFQLEVEGILQSEIMQVFTMDAVKE
                     ITEIMLADQGEVLAPVERKQEERRAQPPLQPEADVVPIQKPAFQELHKSDKQHLPQNL
                     ELILDVPLEFSVMLGESKKTIKDILSLGTGSVVELNKLTEEPLSIYVNGKRIAEGEVV
                     VINENFGIRITNILSKEKRIETL"
BASE COUNT          316 a          216 c          274 g          262 t
ORIGIN      
        1 agttgccgca tccacaacca tcagccaaat ggagagcgac atcatcgggg aagtcggtaa
       61 tatctctatg tcccaagcgg ctacgacatt gtccgaaatt ttaggtcata aagtacacat
      121 cacaacgcct caagtattcg tcaaaagtat ccgtgaggtg atagatgctg cggttaagcc
      181 taaaatcatt acctcgatcg gcttcaaaaa aggtttgacg gggaataatc tcttgctgat
      241 ggatgcgcag gactccgtga tcatcgccaa cttgatgatg ggtggagatg gccaagtaac
      301 cagttcagaa ttaacggaat tagaactgag cgcggtcggc gaagccatga atcagatgat
      361 gggatcttcc gcaacggcca tggcgaccat gctggggaag atgatcgaca ttttgcctcc
      421 ggaagtgcat ttggtagatg ataannnnnn nnnnnnnnnn nnnngaacat tttaatgtcg
      481 atatcggtgt ggataatttg aacgcagatg tctgtctgat cgcattccaa ctggaagtgg
      541 aaggcattct ccagagtgaa atcatgcaag tgttcacaat ggatgcggtc aaagaaatca
      601 ctgaaatcat gttggcggat caaggggaag tgcttgctcc agtcgaacgg aagcaagaag
      661 agcgtcgagc gcagcctcct ttgcagccag aagcagatgt ggttccgatt caaaaacctg
      721 cgtttcagga attgcataaa tccgataaac agcatcttcc acaaaatttg gagctgatac
      781 tggatgttcc gttggaattc agtgtgatgc tgggagaaag caagaaaaca atcaaagata
      841 tcctttcgtt agggacgggc tccgttgtgg aactgaacaa attgacggaa gaacctttga
      901 gcatctatgt gaatggcaag cggatcgctg aaggcgaagt tgtagtgatc aacgagaatt
      961 ttggtatccg catcacaaac attctgagca aagaaaaacg catcgaaacg ttatagaaag
     1021 cagcaccttt ggaaagaggc gctgctttct ttctatttat caatcattcc gtgggctttt
     1081 tctggatt
//
LOCUS       DAOMUJ010000592         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34738_length_1086_cov_1.353055, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000592 DAOMUJ010000000
VERSION     DAOMUJ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34738_length_1086_cov_1.353055"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>580
                     /locus_tag="PLT73_10715"
     CDS             <1..>580
                     /locus_tag="PLT73_10715"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM20880.1"
                     /translation="ILINNWEATGMDFTEQQLLDFAETAYQDGVELFVLDDGWFGGRR
                     HEQAGLGDWFVNRELLPEGIDGLAEKIEAIGLRFGLWFEPEMVNADSDLFRAHPDWII
                     HTPGRPVSHGRHQYVLDFTRSEVVDHIYAMISKILRQAKISYVKWDMNRCITEAYSPS
                     LPAGRQGEVFHRYMLGVYDLYERLTSEFPEILF"
     assembly_gap    581..590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <591..>1086
                     /locus_tag="PLT73_10720"
     CDS             <591..>1086
                     /locus_tag="PLT73_10720"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012391776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM20881.1"
                     /translation="ASGGGRFDPGMIYYSPQGWTSDDTDAVERLKIQYGTSFVYPLSS
                     MGAHVSAAPNEQLHRYTPLETRGNVSAFGSFGYELDLNRLTEQERAIVKEQITFIKKY
                     RKIIHSGTFYRLMSPFDGNYCAWMVVSEDKRTVLVGQYKVLNEVNAPFRRLQLIGLDP
                     STEYV"
BASE COUNT          255 a          242 c          301 g          278 t
ORIGIN      
        1 gattttgatc aataactggg aagcgaccgg gatggatttc acggaacagc aattgctgga
       61 ttttgccgaa acagcttatc aagacggggt ggaactgttc gttctggatg atggctggtt
      121 cggtggccgg cgtcatgaac aagctgggct gggagattgg ttcgtcaaca gggaactgct
      181 gccggagggg attgacggct tggctgaaaa aattgaagcg atcggcttgc ggttcgggct
      241 atggttcgag ccggaaatgg tgaatgcgga tagtgatctt ttccgggccc atccggattg
      301 gatcatccat acacctggaa gaccggtttc ccacgggcgc caccaatatg tccttgattt
      361 cacccgctct gaagtagtcg atcatattta cgccatgatt tccaagatat tgcgccaagc
      421 caaaatttct tatgtgaaat gggatatgaa ccgctgcatc actgaggctt attcgcctag
      481 ccttcctgcc gggagacagg gtgaggtgtt ccatcgctac atgctgggcg tctatgacct
      541 gtatgaacgt ctgacatccg agtttccgga aattcttttt nnnnnnnnnn cgcttccggg
      601 ggaggtcgct ttgatcctgg gatgatctat tattctccgc aaggatggac cagcgatgat
      661 acggatgcag ttgagcgatt gaaaatccag tacggcactt cttttgttta ccccttgagc
      721 tcgatgggag cgcatgtttc cgcagctcct aacgaacagc ttcatcgtta tacaccgttg
      781 gagacacgcg ggaatgtttc ggcattcggc tcattcgggt atgaattgga tctgaacaga
      841 ttgacggaac aggaaagggc aatcgtcaag gaacagatta cgttcatcaa aaagtaccgc
      901 aaaatcattc acagcggcac tttctaccgc ctcatgagcc cgttcgacgg gaactattgt
      961 gcatggatgg tcgtttccga agataaacgg acggtgctgg tgggccaata taaagttctg
     1021 aatgaggtca atgcgccttt cagaaggctg caattgatcg gattggatcc ttcgaccgag
     1081 tacgtc
//
LOCUS       DAOMUJ010000593         1080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34964_length_1080_cov_1.486829, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000593 DAOMUJ010000000
VERSION     DAOMUJ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34964_length_1080_cov_1.486829"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLT73_10725"
     CDS             complement(<1..608)
                     /locus_tag="PLT73_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRM20882.1"
                     /translation="MGNLYKDKLSGSRIGIVFGAFAPCHIGHLEVILKAKKENDGCVV
                     IVCGEDGDCGEPFGLDVYRRFRYMRELFADDNQIYVVMAAGQGIPQKETEWESWLEII
                     NLKIADSLQHSDAQKIWYTGKTMQAEVLEKKSTDEVHLVDTSLYPVSGLNIRNDALRY
                     FNAIALPFRRAFTKKVLVLGAPSGGKTTLVKDLAKLYSCPYS"
     gene            complement(793..>1080)
                     /locus_tag="PLT73_10730"
     CDS             complement(793..>1080)
                     /locus_tag="PLT73_10730"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRM20883.1"
                     /translation="QLADDAIEQLLGIENDVTGSVLYHNVFLEERKAELTELLKMGKL
                     SAVYFASPSAWKRFFSVYDQTEKKPLVFYAIGDTTKKAINDSGYEAVLFQR"
BASE COUNT          253 a          292 c          243 g          292 t
ORIGIN      
        1 aaactgtagg gacaggaata cagtttcgct aaatccttga ccagcgtcgt tttccccccg
       61 cttggagcac ccaaaactag tacctttttt gtgaatgccc ggcggaaagg caaggcgatg
      121 gcattaaaat agcgtaaggc atcgttgcgg atattcagac cgctgaccgg gtagaggctg
      181 gtgtccacca aatggacttc gtcggtggat tttttttcca atacctcagc ctgcatcgtt
      241 ttcccggtat accagatctt ttgcgcatcg ctgtgctgca gcgaatcagc tatcttgagg
      301 ttgatgattt ccaaccaaga ctcccactcg gtttctttct gcggaatgcc ttggccggcc
      361 gccatcacta cataaatctg gttatcatcc gcaaacaatt cgcgcatgta acggaatctc
      421 ctgtagacat ccagaccgaa aggctcgccg caatctccat cttctccgca gacgatcacg
      481 acgcaaccgt cattctcctt cttggccttc aggatgactt ccagatgccc gatatggcaa
      541 ggcgcgaacg ctccgaaaac gatgccgatc cggctgccgc ttaatttgtc cttgtacaga
      601 ttccccatct caattctccc ttctgcagaa cgtattttct agggtactca tcttacctct
      661 tacatacatt ctacaacttc ctcaccgcac cgtatagcat gaaggtcctt tccaaaggct
      721 ggatatccgg aaaaaggacg ctccaatgaa aaaaaaggcg ctcagatcga gttgatcttt
      781 gcgcctgctt gcttatcgtt gaaacagaac agcctcgtag ccgctgtcat tgatggcttt
      841 ttttgtcgta tccccgatgg cataaaaaac gagcggtttt ttttcggttt ggtcgtagac
      901 cgaaaagaat cttttccatg ccgatgggct tgcgaagtaa acagcggaca gtttgcccat
      961 tttgagcagc tcagtcaatt ccgctttgcg ttcctccagg aagacattgt ggtacaggac
     1021 cgaaccggtt acatcgtttt cgatccccag gagttgttcg atggcatcat cggctagctg
//
LOCUS       DAOMUJ010000594         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_34986_length_1079_cov_1.949219, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000594 DAOMUJ010000000
VERSION     DAOMUJ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34986_length_1079_cov_1.949219"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..752
                     /locus_tag="PLT73_10735"
                     /pseudo
     CDS             1..752
                     /locus_tag="PLT73_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709958.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            758..>1079
                     /locus_tag="PLT73_10740"
     CDS             758..>1079
                     /locus_tag="PLT73_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM20884.1"
                     /translation="MKKNNISKYFAYAFLILLALVWLFPALFGLFTSFKSQGDIMEVG
                     FRMFPANWIVTNYLKLLQNTSSAPIITWFFNSLFVSTTHALLVVVVVSLAAFGYTRVN
                     FKGRD"
BASE COUNT          245 a          249 c          255 g          330 t
ORIGIN      
        1 gatcagactt gggtaggttt gaacaattat aagacgattc tgttggatca ggattcgacg
       61 ttctatttcc aattccgaaa cggtctgaaa aataccttgc tctttgtact ctacacgatt
      121 ccattccaaa ttctttttcc gttgctgatc gcgaccgtca tgcagcataa aggtctgaag
      181 ttcaaaggtc tgttccaggc tatcttttat gtccctgggc tcgtttcagc ttcagcaggt
      241 gctttgattt ggctattgat ctttaatcca cgattgggtc cgatgaacaa cttggtcaat
      301 tccgatattg tttggacagc gcaacagcct tatgcttgga tcgtcatttt cgtgatgtcg
      361 atgtggggag cgataggcgg caatctgatc atctaccgtt ccgctatggc tggcgtgtcg
      421 gaagacctat atgaagctgc tgagatcgat ggcgcggtgc actcaggaaa ttcatcagta
      481 ttacgcttcc ttccatccgt ttcccattat tttatacatt cgtcatgtcc actgccgggg
      541 ctttcaatgt gtacgttcaa cctttgatgg caacaaacgg gggaccgaat cagtccacca
      601 cggtcctgat gatgtatatc cgcaatctgg cattcggatc gggtgaatcc gttgcgggta
      661 tggcttcagc aatggccgtc ttattagggt tagtcattct cgcagtatca gccctgcaat
      721 tctatgtcat gacgaaaaga tcgaaggggt gaaataaatg aaaaaaaata atatttccaa
      781 atattttgcg tatgctttcc tgattctgtt ggcacttgtc tggttgttcc cggcgctgtt
      841 cggactattc acgtccttca aatcccaagg agatatcatg gaagtgggct tccggatgtt
      901 ccctgccaac tggatcgtca ccaattacct gaaattgttg cagaatacat cgagtgcacc
      961 gatcatcacc tggttcttca actccctgtt cgtttcgaca acgcatgcct tactggttgt
     1021 ggtggtcgtc tcgctggctg cgttcggcta cacaagggtc aatttcaaag gacgggatg
//
LOCUS       DAOMUJ010000595         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_35034_length_1078_cov_1.715543, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000595 DAOMUJ010000000
VERSION     DAOMUJ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35034_length_1078_cov_1.715543"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1078)
                     /locus_tag="PLT73_10745"
     CDS             complement(16..>1078)
                     /locus_tag="PLT73_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM20885.1"
                     /translation="GEPDFDTPDFIKAAATEALNQNQTHYAPSAGVLETRKEIAKFME
                     RKYGLHYNPETDIVLTVGATEAITASMFALMNPGDKVIIQSPAFPQYEYVTHLAGGHS
                     VKIDTSETGFLITPEDLTAALDANPETKMIVLTYPNNPTGATYTKEEVEALAAVIRKY
                     DVFVLSDEIYSELTYDGGHTSIAEFIPEQSIVINGTSKSYAMTGWRVGFVAAQADLIR
                     EILKYHQSLVTCGSTPAQMAAGVAFRDGDEVTATMKAAYRKRRDILLEGLTAAGLKMN
                     NPDGAFYLFPRIPEQYGDDDWNFCIDLARKAGVGVIPGSAFGESGVGYFRMSYAASDE
                     NVIEGCRRIVAFLEELNKK"
BASE COUNT          222 a          298 c          292 g          266 t
ORIGIN      
        1 tcaacattta ttcccttatt tcttgttcaa ttcttccagg aaggcaacga tccttcggca
       61 gccttcgatg acattctcat cgctggcggc gtagctcatg cggaaatagc cgactcctga
      121 ttcgccgaaa gccgatccag ggatgacgcc gacgccggct ttacgcgcga ggtcgatgca
      181 gaagttccaa tcatcgtcgc cgtattgctc gggaatgcgc gggaacaggt agaaggcacc
      241 atccggattg ttcatcttca gaccagcagc agtcagacct tccaacagga tgtcccggcg
      301 tttgcggtag gccgctttca tcgtggctgt cacttcatcg ccgtcgcgga aggccacgcc
      361 agccgccatt tgtgcaggcg tggaaccgca tgtgaccaga ctttggtgat atttcaaaat
      421 ttcgcggatc aagtctgctt gggcagcgac aaagccgacg cgccagccgg tcatcgcata
      481 gcttttcgaa gtgccattga tcacgatgga ctgctcaggg ataaactcgg caatggatgt
      541 atgaccaccg tcgtaggtca gttcgctgta gatctcatcg cttagcacga aaacatcata
      601 tttcctgatg accgcggcca atgcttcgac ttcttctttt gtgtaagtcg cgccggtcgg
      661 attgttggga taggtcagca cgatcatctt cgtttccgga ttcgcatcca acgccgccgt
      721 caagtcttcc ggtgtgatca ggaagccggt ctccgaagtg tcgatcttga cgctgtgccc
      781 gcccgccaaa tgcgtaacgt attcatactg cggaaaagcc ggcgattgga tgatcacctt
      841 atcgcccggg ttcatcagcg cgaacatcga ggcggtgatc gcttccgttg cgccgaccgt
      901 caggacgatg tcggtctccg ggttgtagtg caagccgtat ttccgctcca tgaattttgc
      961 gatttccttg cgggtctcga gaacaccggc agaaggagca tagtgcgttt gattctggtt
     1021 caaagcctct gtggcggcag ccttgatgaa atccggcgta tcgaaatcag gttctccc
//
LOCUS       DAOMUJ010000596         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_35118_length_1076_cov_1.597453, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000596 DAOMUJ010000000
VERSION     DAOMUJ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35118_length_1076_cov_1.597453"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLT73_10750"
     CDS             <1..431
                     /locus_tag="PLT73_10750"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM20886.1"
                     /translation="RATFRQDFYALPNIIANFLQSQPLSGLFATFTFRYGEQMVSTEA
                     LGYFGFLGFFGSKIWLFLSYFILTYLWYGGLSGKMRDVVTPVVVALLLSVSYAAADEF
                     HQSLVSPAFAMKEDVVLAGAGAVLAVITGWFFFTVRKGRK"
     gene            498..>1076
                     /locus_tag="PLT73_10755"
     CDS             498..>1076
                     /locus_tag="PLT73_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM20887.1"
                     /translation="MSGHSKWSKIKGAKGAADQKRGKIFQRLSKEIYMAAKNSGPDPS
                     SNPSLRLMIDKAKAANMPNDNITRAIKKATSAGEGENYEEVTYEGYGPNGVAVLVHAL
                     TDNRNRTATNIRVAFNKNGGSLGESGSVSYMFDRKGYIAIEREDLDVDEDTMLMSVLE
                     AGGEELQTSDEVFEIYTDPADFDAVSEALKAEG"
BASE COUNT          296 a          212 c          279 g          289 t
ORIGIN      
        1 accgtgccac tttccgacaa gatttttatg ctttaccgaa catcatcgcg aatttcctgc
       61 aatcgcaacc gctctccggg ttattcgcaa cattcacctt ccgatacggt gagcagatgg
      121 tcagcacgga ggctttgggc tatttcggtt ttctgggatt cttcggcagc aagatatggt
      181 tgttcctttc ctatttcatc ttgacttatc tttggtatgg cggtctttcc gggaagatgc
      241 gggatgtagt tacacctgtt gttgtggcgc tgttgctttc cgtcagttac gcggctgcgg
      301 atgaatttca tcaatcgctg gtgtctccgg catttgcgat gaaggaagat gtggtcttgg
      361 ccggcgcagg agcagttttg gcggtaatca ccggttggtt cttctttaca gtcagaaaag
      421 gcagaaagtg aattgccata agcggttact tttgataaaa tgattgagta aattaataga
      481 tgagaagagg gatctgaatg tcaggacatt caaaatggag taaaatcaaa ggtgcaaaag
      541 gcgctgcaga tcaaaaacga ggtaaaattt ttcagagatt atcgaaagaa atttatatgg
      601 ctgcaaaaaa cagcggtccg gatccctcat ctaatccgag cctgcgctta atgatcgata
      661 aagcgaaagc agcgaacatg ccgaatgata acatcacgcg tgccatcaaa aaagcaacat
      721 ctgctggaga aggcgaaaat tatgaagaag tcacttacga aggatacggt ccgaacggtg
      781 tagccgtttt ggtccatgct ttgacagaca accgcaaccg taccgccact aatatccggg
      841 ttgctttcaa taaaaatgga ggatctttag gtgaatcagg ttcggttagt tacatgttcg
      901 accgtaaagg ctatatcgca atcgaacgtg aagacttgga tgtggatgaa gatacaatgt
      961 tgatgagcgt attggaagcc ggtggagaag agctgcagac gagtgatgaa gttttcgaaa
     1021 tctacacgga tcctgccgat tttgatgctg tttccgaagc tttgaaggca gaaggc
//
LOCUS       DAOMUJ010000597         1072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_35267_length_1072_cov_2.144543, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000597 DAOMUJ010000000
VERSION     DAOMUJ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35267_length_1072_cov_2.144543"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..>1072)
                     /locus_tag="PLT73_10760"
     CDS             complement(175..>1072)
                     /locus_tag="PLT73_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRM20888.1"
                     /translation="EAAVVRMVQAVRILRASINSPKESVEAVSPVIVPQYIMATGLWT
                     KEQIKLAFHLYCQLPYGRIHGRNPEIMALAKVIGRTADAVAMKMLNLASIDPAVVSSG
                     RAGLGNASSLDRAVWDEFHSDWERLAVECQMLRQQLDKDTVDGLEPEGGGDVLAQEDF
                     TGETRQVVTAQRIKQHFFRRAVLSSYRNRCCMSGLTDSRLLIASHIVPWSSDKANRLN
                     PSNGLCLSAIHDRAFDKGLICLADDFTIIVSEDLRRSKDVFVKDVLLPLNGQKIEPPE
                     RFAPQPEFVAWHRNSIFVDNQK"
BASE COUNT          256 a          289 c          265 g          262 t
ORIGIN      
        1 caacaatggg aatgatgcgt tggcactcca gacaatcaca aacaaagacg cgctggttgt
       61 cgatttttgt tatatcaagc agcatcaatc tggacccgca cctgtggcaa gtatcaggtt
      121 gctcggtcat cacttgataa acagtatctg gcggaaattg ccatttcata gcagctactt
      181 ttggttgtcg acaaatattg aatttcgatg ccacgcaaca aactcgggtt gtggcgcgaa
      241 tcgctcgggt ggctcaattt tttgtccatt gagaggcagc aatacgtctt tgacgaaaac
      301 atccttactt cgcctcagat cctcagaaac gatgatcgtg aaatcatcag caaggcaaat
      361 cagacctttg tcaaaagcgc gatcgtgaat ggccgacaag caaagcccat tgcttgggtt
      421 gagcctgttt gccttgtcgc tgctccaagg aacaatgtgg ctggcgatca ataaccgtga
      481 atccgtcagt cctgacatgc agcaacggtt tctgtaactg ctgagcacgg cccggcggaa
      541 aaaatgctgc ttgattcgct gcgctgtcac cacctgccgg gtctccccgg taaagtcttc
      601 ctgtgctagc acatcacctc ctccttctgg ctccaagcca tcgacggtgt ccttgtccag
      661 ttgctggcgc agcatctggc attccaccgc caatcgttcc cagtccgaat ggaattcgtc
      721 ccaaactgca cggtctagcg acgaagcatt tcccaaccct gcacgcccag agctaacaac
      781 ggccgggtca atactggcaa ggttgagcat tttcatcgcc actgcatcag cggtacgtcc
      841 aatgactttg gccagcgcca tgatttccgg atttcgcccg tgaattcttc cgtaggggag
      901 ttggcagtaa agatgaaaag ccagcttgat ttgttctttt gtccaaagac cagttgccat
      961 gatgtattgc ggcacgatga cgggagaaac cgcttcgacc gattccttgg gtgaattgat
     1021 ggacgcgcgc agtatgcgga ccgcttgaac catgcgaacc acggcagctt cg
//
LOCUS       DAOMUJ010000598         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_35320_length_1071_cov_1.760827, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000598 DAOMUJ010000000
VERSION     DAOMUJ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35320_length_1071_cov_1.760827"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>1071)
                     /gene="gshAB"
                     /locus_tag="PLT73_10765"
     CDS             complement(189..>1071)
                     /gene="gshAB"
                     /locus_tag="PLT73_10765"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HRM20889.1"
                     /translation="EYVKNGNMTAKDSYIGPLIMENKVVTKKILAENGFAVPDSGEYH
                     SAAAALRDYSIFAGKGIVVKPKTTNYGLGISIFKDGASFEGYEKAIHMAFEEDEDVLV
                     EDYLSGTEYRFFVIGNETKAVLLRVPANVVGDGSQTIRQLVEEKNKDSLRGKNHRTPL
                     ALIGTGELEKLTIQGQGYTFDSIPPAGVTVRLRDNSNISTGGDSIDVTDQMNDSYKKL
                     AVQMAAALGVAVCGVDIIIPDHSVPATQAEPNYGVIEANFNPAMLIHIYPYKGKSRRL
                     TMEILRLLFPEMHLEEE"
BASE COUNT          251 a          290 c          248 g          282 t
ORIGIN      
        1 ggaggaggaa gtgcacatag acggatagga acaaacctgc cgacagtatc gggataagga
       61 ggggatcaaa tgacaacaga ttcccccaat tgttgaatct cttcgcaatc agatatccgt
      121 acgccataat gacggaaact gaaatgtaga aggcaattct cagaaatgac tttttcatac
      181 agttccccct actcttcctc caggtgcatt tccgggaaca ataatcgcag gatttccatc
      241 gtcaaacggc gacttttccc tttgtacgga taaatatgga tcagcatggc cgggttgaag
      301 ttcgcctcga tcaccccata atttggctcc gcttgcgtgg ccggaaccga atgatccggg
      361 atgatgatat ccactccaca gaccgcaaca cccagtgcgg ctgccatctg cacggccagt
      421 tttttatagc tgtcgttcat ctgatccgtg acatcaatcg aatccccacc ggtactgatg
      481 ttcgaattgt ccctcaaccg gacagtcact cctgcgggcg gaatgctgtc gaaagtatac
      541 ccttggcctt ggatcgtcaa cttctcgagt tccccggtcc cgatcaatgc taacggtgta
      601 cgatgatttt tgccgcgcaa gctgtctttg ttcttttcct cgaccaattg gcggattgtc
      661 tggctgccgt cgcccaccac attggcgggt acgcgcagca atacagcctt cgtttcgttt
      721 ccgatgacaa agaaacggta ctcggtgccg gacaagtaat cttccacgag cacatcttcg
      781 tcttcttcaa atgccatatg gatggctttt tcataaccct caaaggatgc gccatccttg
      841 aagatggaaa tccccagacc gtagttcgtg gtctttggtt tgacgacgat gcctttcccg
      901 gcaaagatgg aataatcacg gagagctgct gccgcagaat gatactcgcc gctgtccggc
      961 accgcgaatc cgttctccgc caatattttt ttggtgacga ctttgttttc cataatgagc
     1021 ggaccgatat agctgtcctt tgccgtcata ttgccattct tcacgtattc g
//
LOCUS       DAOMUJ010000599         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_36487_length_1044_cov_1.263903, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000599 DAOMUJ010000000
VERSION     DAOMUJ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36487_length_1044_cov_1.263903"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLT73_10770"
     CDS             complement(<1..261)
                     /locus_tag="PLT73_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRM20890.1"
                     /translation="MEQSFVREMTSKHLVMLSLGGVIGTGIFLSSGYLIQTAGSIGTI
                     IAYLIGAFLVTLVMMCLGELSVHEPSTSSFHNYASKYIHPGAG"
     misc_binding    complement(314..541)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(904..>1044)
                     /locus_tag="PLT73_10775"
     CDS             complement(904..>1044)
                     /locus_tag="PLT73_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRM20891.1"
                     /translation="AKDMGIIYKQYGISPDRVANVVAFAIDQPEDTNVNEFTIGPTIQ
                     PW"
BASE COUNT          254 a          258 c          232 g          300 t
ORIGIN      
        1 acctgctccg ggatggatat attttgaagc gtagttgtgg aatgaactcg tgctaggctc
       61 gtggaccgac aattcgccca agcacatcat gaccaacgtc accaaaaatg ctccgatcag
      121 atacgcaatg attgttccga tcgaacccgc cgtttgaatg agataccccg aactcaagaa
      181 gattccagtt ccgattaccc cacccaatga aagcataact agatgctttg atgtcatttc
      241 ccttacaaat gattgctcca tattttcgcc tccaaaaatg ttttaaaaat atataaaaaa
      301 agcactcccc tattgaaagg gcgagtgctc gcgttaccac ctttgttcat aatcaaaaag
      361 attatctcag caagggactg tcatcccttc acgcgctatc gggcgtaccc gtcacttgct
      421 aagtttccct cacacgtgat aactcaaaga ccattttcgc tgattccgaa acatctgctt
      481 tcaccattcg cagactctct gtggtttctt tcccagctac ttatctcatc aaagtttcat
      541 atatttttaa taaatataca agaatgatgg atggttgtca attattttgt gatatatttt
      601 gtgcttactt tcattcttgt acgtttcggt tggagttgtg tggcggcggc cggaggtggc
      661 tgtacgtttt ggtgctgtcc tccggctaag cggttgtggc cggagatacg ataccggtca
      721 cgtcgctctg ctccggcggg gagacgctgg acggagaaca gagtcgtttt tgacgctctc
      781 ctccgtccaa gcattctttc accggaggag agttcccccc ggtaacattc gaaaaaaact
      841 ataaagcaag cccgccgcac ccctttcagt tgggcgcaga aggcttgctg ttactgactt
      901 ttatcaccaa ggttgaatcg tcggtccgat agtaaattcg ttgacgttcg tatcctcagg
      961 ctggtcgatc gcgaaggcga caacattggc tacgcgatct gggctgatgc cgtattgttt
     1021 gtagatgatt cccatgtctt tggc
//
LOCUS       DAOMUJ010000600         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_36503_length_1043_cov_1.953441, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000600 DAOMUJ010000000
VERSION     DAOMUJ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36503_length_1043_cov_1.953441"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PLT73_10780"
     CDS             <1..865
                     /locus_tag="PLT73_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM20892.1"
                     /translation="ADAGSFNKASEELYVSPNAVMKQINLLENSLGFDLFVRTHRGVR
                     LTPAGESLYRDAKYLIQYAKDSVARAEKMVAEPKHVLRIGTSLTTPVQFLVSLWPQIR
                     QHNPDLKFELVSFENTPENAREIMNHFGRNIDVVAGIYSDNILSERRCSALKLYDTPL
                     CAAVPLHHRLASNDEIAIEDLFDEKVMLIKRGYMSAFDEVRDNLAENYPRIQIEDFSF
                     FDVNVFNKCENDNAVLIGVKEWGNVHPLLKIIPISWKHTIPMGILHSNTPSKDVKKFL
                     QIISRIYEVQS"
BASE COUNT          291 a          225 c          273 g          254 t
ORIGIN      
        1 cgccgatgcg ggcagtttca ataaggcgtc cgaagaactg tatgtatcac cgaatgctgt
       61 catgaaacaa atcaatcttc tggaaaacag tctgggattt gacttgtttg tccggacgca
      121 tcggggcgtc cgcttgacac cagcaggcga atccttgtac cgggatgcga agtatctcat
      181 ccaatatgcc aaagattccg tcgcgagagc cgaaaagatg gtggcagagc ccaagcacgt
      241 actaaggatc gggacctcat tgacgacgcc ggtccaattt ttggtcagtc tgtggcccca
      301 gatccgccaa cataatccgg atctgaaatt tgaactggtc tcattcgaga acaccccgga
      361 gaatgcgcgg gaaatcatga atcatttcgg caggaacatc gatgtggtcg ccggcatcta
      421 cagcgataat atattgagcg agcggcgttg ttcggcattg aaattgtatg atacacctct
      481 ttgtgcggcg gtcccgctcc atcatcgctt ggcatcaaat gatgaaatag cgatcgagga
      541 cctgttcgat gaaaaggtca tgctcatcaa acgcggctac atgagtgcct ttgatgaagt
      601 cagggataat ctggcagaaa actatccgag gatccagatt gaagattttt catttttcga
      661 tgtgaatgtc ttcaacaaat gtgaaaatga caatgcggtt ctgatcggtg tgaaggaatg
      721 gggaaatgtc catcctttgc tgaaaatcat tccgatcagc tggaagcata cgattccgat
      781 ggggatcctg cattcaaaca cgccatccaa ggatgtcaaa aaattcctgc aaatcatcag
      841 tcggatatac gaggtccagt cctaaccttt gggttgcaac ttgtgagcca agagagactt
      901 cttaatagtc tcttttttac tatactgaag aggtaatagt aagaaggggg aaacagagat
      961 gaaaacaaga gtattgggac aagggttgga agtatcggca atcgggctgg gctgcatggg
     1021 gatggatcat gcctacggtg cac
//
LOCUS       DAOMUJ010000601         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_36647_length_1040_cov_1.623350, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000601 DAOMUJ010000000
VERSION     DAOMUJ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36647_length_1040_cov_1.623350"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="PLT73_10785"
     CDS             complement(<1..>1040)
                     /locus_tag="PLT73_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRM20893.1"
                     /translation="ASVVFKEWAENARAHKIVYDGKRTMVMLHYAGIALAGIDFDILL
                     ASYILNAKDNSKDLAQVAQEYGYNDVSFDEAIYGKGAKTAVPEDAILHDHLARKVKAI
                     QALFAQLNAELESNVQKDLYYEMELPLSFVLAHMEIQGIKVEPQRLLTMKEEFAGRLL
                     EIEKTIYAEAGEEFNINSPKQLGVILFEKMGLPAIKKTKTGYSTAVDVLEKLQAQAPI
                     VEHILNYRQLAKLQSTYVEGLLKFIKPETGKIHTRYQQTLTQTGRLSSVDPNLQNIPI
                     RMEEGRKIRQAFVPQQAGWKLFASDYSQIELRVLAHISGDEHLREAFLEGQDIHTSTA
                     MRVFGIEKPEDVT"
BASE COUNT          215 a          279 c          259 g          287 t
ORIGIN      
        1 gtgacgtctt ccggtttctc gatgccgaat acacgcatcg ctgtcgaagt atggatatcc
       61 tggccttcca ggaaggcttc gcgcagatgc tcatccccgg aaatatgcgc cagcacacgc
      121 aattcgattt gtgaatagtc ggaggcgaac agcttccaac ctgcttgttg cggaacgaaa
      181 gcttgtctaa ttttccggcc ttcctccatc cggatcggga tgttctgcag gttcggatcg
      241 acggaactca aacgaccggt ctgcgtcaat gtctgttgat aacgggtatg gatctttccg
      301 gtttccggtt tgatgaattt caggagtccc tccacgtagg tggattgcag cttggccaac
      361 tgtcggtaat tcaggatgtg ctccacgatc ggcgcttgcg cctgcagttt ttccaagaca
      421 tcgaccgctg tcgaatagcc cgttttcgtc tttttgatgg cgggcaagcc catcttctcg
      481 aacaggatga cacccagctg cttcggtgag ttgatgttga actcctcacc cgcctcggca
      541 tagatagttt tttcgatttc aagcaagcgg ccggcaaatt cttctttcat ggtcaacagg
      601 cgctgcggtt ccactttgat cccttggatt tccatgtgcg ccaagacaaa cgacaacggc
      661 agctccattt cgtaatacaa atctttttgc acgttgcttt ccagttcagc gttcaattgg
      721 gcaaacagcg cctggatcgc tttcactttg cgcgctaaat ggtcatgcag tattgcatcc
      781 tcgggaaccg ccgttttagc gccttttccg tagatggcct catcaaagga gacatcgttg
      841 tagccgtatt cctgggcgac ttgcgcaagg tctttgctgt tgtccttcgc attcaggatg
      901 taggaagcca gcaggatatc aaagtcgatg cctgccagcg cgataccggc gtaatgcagc
      961 atgaccatcg tccgtttgcc gtcgtacaca attttatggg ctcgggcatt ttcagcccat
     1021 tccttgaaaa caacacttgc
//
LOCUS       DAOMUJ010000602         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_36994_length_1032_cov_1.967247, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000602 DAOMUJ010000000
VERSION     DAOMUJ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36994_length_1032_cov_1.967247"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLT73_10790"
     CDS             complement(<1..295)
                     /locus_tag="PLT73_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HRM20894.1"
                     /translation="MDKKEIVDEALKTQVDSLLETIADDTGSEKNDHKEEEIVKLLDD
                     NRLLVGTQEYELSIDHREGFDPEALAGRYTSILSKYDYIVGDWGYDQLRLKGFY"
     gene            complement(305..>1032)
                     /locus_tag="PLT73_10795"
     CDS             complement(305..>1032)
                     /locus_tag="PLT73_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRM20895.1"
                     /translation="GKFGKYIGHVTVSYNSDRILDRKAELIEAPTLPEETGDRAEVEE
                     LQERGENLLSAIPIADNPVKLKADEGHINDLLLLGLDAVTEYAGTPFAILNSGLFLED
                     LPEGIITKNHLHQILPHPMRLLECTLTGRDFKAMIEEMESQREELMTKEITGMGFRGR
                     IFGELCYRGFEVDPLTNHVIVGGKQISDEEDITFVTVDHFRYISYFPTIENEGRSRLL
                     FPYFLRDVFGLHLENKFPIMREM"
BASE COUNT          260 a          277 c          198 g          297 t
ORIGIN      
        1 tgtaaaaacc cttcagacgc agctgatcat agccccaatc cccaacgata taatcgtact
       61 tactcaaaat ggaggtatat cttccggcta acgcttcagg atcaaagcct tcacggtgat
      121 caatgctcaa ttcgtattct tgggttccca ccaacagacg gttatcgtcc aataacttaa
      181 caatttcttc ttctttgtgg tcgttctttt ccgacccagt atcatcagca atagtttcca
      241 gcaaggagtc tacttgcgtt ttcaatgcct catcaactat ttctttttta tccacgatag
      301 cacctcacat ttctctcatt atgggaaact tattttccaa atgcagaccg aacacatccc
      361 ttaggaaata cgggaacagc agtcggctgc ggccttcgtt ttcaattgtc gggaaatagc
      421 tgatgtaacg gaaatggtcc accgtcacaa aggtgatgtc ttcctcatcg gaaatctgct
      481 tacctcccac tatcacgtgg ttggtgagcg gatcaacttc gaacccccgg tagcagagct
      541 ccccgaaaat tcttcctcgg aaacccatgc cggttatttc cttcgtcatc agttcttcac
      601 gttgcgattc catttcctcg atcatagctt taaaatcccg tcctgtcagc gtgcattcca
      661 gtaagcgcat cggatgcgga aggatttgat gcagatggtt tttggtgatg atcccttccg
      721 gtagatcctc caagaacagt cctgagttca gtatagcaaa tggcgtaccg gcatattcag
      781 tcaccgcgtc caaacccaaa agtaataaat cattgatatg accttcatct gctttcaatt
      841 taaccggatt atccgcaatc ggaatcgcag acaggagatt ctctccgcgt tcttgcagtt
      901 cctcgacttc cgcacgatcg ccagtttctt ccggaagagt aggtgcttca atcaactcgg
      961 cttttcgatc caaaatcctg tcggaattat aactgaccgt gacatgcccg atgtatttcc
     1021 cgaacttacc ag
//
LOCUS       DAOMUJ010000603         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37109_length_1029_cov_2.045175, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000603 DAOMUJ010000000
VERSION     DAOMUJ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37109_length_1029_cov_2.045175"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="prmC"
                     /locus_tag="PLT73_10800"
     CDS             <1..608
                     /gene="prmC"
                     /locus_tag="PLT73_10800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM20896.1"
                     /translation="WFYGYPLKVMEATLIPRPETEELVQRALKCLEGKGPQKVLDIGT
                     GSGAIAIAMKKQRPQDEVTATDLSQEALVVAEENADQLDAQIRFLQGDLLEPVQLETF
                     DCILSNPPYISEDEISLMDSSVLEYEPHSALFADHDGLDLYQKMAFTLPFHLKTDGCL
                     FMEIGFNQGEKLLELYKKAFPDKTVTIEKDLSGLDRMLIVK"
     gene            806..>1029
                     /locus_tag="PLT73_10805"
     CDS             806..>1029
                     /locus_tag="PLT73_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008471833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRM20897.1"
                     /translation="METKIYDASTIDEAAELLKAGDLIAFPTETVYGLGAIASNDEAV
                     KNVYKVKGRPSDNPLIVHVSDREIAKYVAFV"
BASE COUNT          294 a          232 c          263 g          240 t
ORIGIN      
        1 aatggtttta cggataccct ttgaaagtca tggaagctac attgattcca cgtccggaaa
       61 cggaggagct tgtgcagcgg gctttgaaat gcctggaggg gaaaggtccc cagaaggtcc
      121 tggacatcgg gacgggatcc ggagctatcg ccattgcgat gaaaaagcaa cgtccgcagg
      181 atgaagtgac cgccactgat ctttctcaag aagccctggt cgttgctgag gagaacgcgg
      241 atcaactgga tgcgcagatc cgcttcctgc aaggggatct gctggagccg gtacaactgg
      301 aaacgttcga ctgcatcctc agcaatcctc cctacatcag cgaagatgaa attagcctga
      361 tggacagttc tgttttggag tacgaaccac attcggcgtt gtttgctgat catgacggat
      421 tggatctata ccaaaagatg gccttcacgc tgccgttcca tttgaaaacg gacggatgcc
      481 tattcatgga aatcggcttc aaccaaggtg aaaaattgtt ggaactctac aaaaaagctt
      541 tcccggataa aaccgtcacg atcgaaaagg atctgtccgg tctcgaccgc atgctgatag
      601 taaaataaga aaagctgaac aggtttcttt gttctttctg aagggatgaa ccgtgaagct
      661 agccatcagg tttattgtga ataagatgaa aagagtcgcc tgatatccat gggcaaaaaa
      721 tggctgcggg caattgcccc ggctattttt ttacctgatc agcgccttcg ccagccaaaa
      781 cattgagaaa ggagcataca gtattatgga aacaaaaata tacgacgcct caacaatcga
      841 tgaggcagcc gagctgttga aagccggaga cttgattgct ttcccgaccg agactgtcta
      901 cggcctaggc gccattgcat caaatgatga agccgtaaaa aatgtataca aagtgaaagg
      961 aagacccagt gataacccct tgatcgttca tgtatccgat cgcgagattg cgaaatatgt
     1021 ggcctttgt
//
LOCUS       DAOMUJ010000604         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37185_length_1028_cov_1.279548, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000604 DAOMUJ010000000
VERSION     DAOMUJ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37185_length_1028_cov_1.279548"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLT73_10810"
     CDS             complement(<1..634)
                     /locus_tag="PLT73_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRM20898.1"
                     /translation="MEFVIENEHLIVTVKDKGAEVSSVIAKKTGIEYIWQADEKVWNR
                     HAPVLFPFVGRLKEDSFRYKGNTYPMGQHGFARDSKFSVHERLTDSITFILAPNGHFK
                     DVYPFLFELQLTYQLFENQLSVIYKVKNLDENTMHPGFNVPLTEGEAFEDYYVKMTPE
                     TSRERLLLEGPYLAADKALQVEQNNFPLQRELFLNDAIILKTPEPTTVTLA"
     gene            complement(671..>1028)
                     /locus_tag="PLT73_10815"
     CDS             complement(671..>1028)
                     /locus_tag="PLT73_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRM20899.1"
                     /translation="GATVIGIELLHSINLRTEEATRLTTLIQIAIKSLSFSELEAVKA
                     IFESFPSLEERITASKIAEEKNITRSVIVNALRKLESAGILETKSLGMKGTFIRVVSP
                     ELLDALKKALNQRNDQ"
BASE COUNT          267 a          240 c          206 g          315 t
ORIGIN      
        1 atgccaacgt caccgtcgtc ggttccggtg ttttcaaaat aatggcatca ttcaagaata
       61 gttctctttg cagcgggaaa ttgttctgct ccacttgaag tgctttgtct gcggctagat
      121 acggcccttc cagcaataag cgctctcggg aagtttcagg agtcatcttg acgtaataat
      181 cttcgaatgc ctcaccctcc gtcaacggaa cgttaaagcc tggatgcatc gtattttcat
      241 caagattttt caccttgtaa atgacggaca actggttctc gaacaattga taagtcaact
      301 gcaattcaaa caaaaatgga taaacatctt tgaagtgacc gttcggcgca agaatgaacg
      361 taatgctgtc agtcagacgc tcatgcactg aaaatttcga gtcgcgggca aaaccgtgtt
      421 gtcccattgg ataagtgtta cctttatatc ggaaggagtc ctccttcagg cgtcctacga
      481 aaggaaatag gacaggggca tgtctgttcc aaactttctc atcagcttgc caaatatatt
      541 cgataccagt tttctttgcg attacactgg atacttctgc acctttatct tttacggtta
      601 ctataaggtg ctcattttcg attacaaact ccatgacatt tccccctagt ttggctttac
      661 accaatttga ttactgatca tttctttgat tcaaagcctt ttttaaagca tcaagcaatt
      721 ctggggaaac gactctgatg aatgtcccct tcatgccgag cgatttcgtc tcgagaatgc
      781 cggctgattc caacttcctt aaagcattca caatcacgga gcgcgttata tttttttctt
      841 ccgcaatttt cgatgcggtg atgcgttcct ctaaggatgg aaaactctca aaaatagctt
      901 tgactgcttc cagttcgctg aaagacaagg attttattgc aatttggatc agtgtcgtca
      961 atcttgttgc ctcttccgta cgcaaattga tgctatgcag caactcaata ccgattacgg
     1021 tggccccg
//
LOCUS       DAOMUJ010000605         1027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37229_length_1027_cov_1.599794, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000605 DAOMUJ010000000
VERSION     DAOMUJ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37229_length_1027_cov_1.599794"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>338
                     /locus_tag="PLT73_10820"
     CDS             <1..>338
                     /locus_tag="PLT73_10820"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM20900.1"
                     /translation="VSKGINVQKGHTIVINIDVEQAPFARLLTKEAYALGAEEVIVKW
                     TDDFVTRETYLHTSEERMTNVPQYKIDESLDMIEKKASRLSVRSADPDALNGVDGKKL
                     AAVQKAHNVA"
     assembly_gap    339..348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <349..>1027
                     /locus_tag="PLT73_10825"
     CDS             <349..>1027
                     /locus_tag="PLT73_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002914068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM20901.1"
                     /translation="RTATQANKVSWTVAAAAGAKWAAKVFPDLATEEEQVDALWNEIF
                     KTCRVYEADPVAAWDAHEARLLSKADVLNAEQFDALHYTAPNGTDLTVGMPQNHIWES
                     AGSVNAQNEKFIANMPTEEVFSAPDFRRIDGVVKSTKPLSYAGNIIDGMTFRFANGQV
                     TEVTAEKGEDTIKRLVEENDGGRSLGEVALVPDPSPISQSGIVFFNTLFDENASNHLA
                     LGSAYAS"
BASE COUNT          258 a          289 c          282 g          188 t
ORIGIN      
        1 gtttcgaaag ggatcaatgt acaaaaaggc cacacgatcg tcatcaacat cgacgtcgag
       61 caggcaccgt tcgcgcgtct gttgacgaag gaagcctacg cgttgggcgc cgaggaagtc
      121 atcgtcaagt ggaccgacga tttcgtcacc cgcgaaacct acctgcacac gtccgaggag
      181 cgcatgacga acgttccgca gtacaagatt gatgaaagcc tcgacatgat tgaaaagaaa
      241 gccagccgcc tttcggtacg ctcggccgat ccggatgcgc tgaacggcgt cgacggcaaa
      301 aagttggctg ctgtccaaaa agcccataac gtcgccttnn nnnnnnnnaa cgcaccgcga
      361 cccaagcgaa caaagtgagc tggaccgtcg ctgccgctgc cggcgcgaaa tgggcagcga
      421 aagtcttccc tgatctggct acggaagaag agcaagtcga cgctttgtgg aacgaaatct
      481 tcaagacatg ccgcgtctat gaagccgatc ctgtggctgc ctgggatgcg catgaagcgc
      541 gcctgttgtc gaaagccgat gtgctgaatg ccgagcaatt cgacgctttg cattacactg
      601 cgccgaacgg aaccgatctg actgtcggta tgccgcaaaa ccacatctgg gaaagcgccg
      661 gcagtgtgaa cgcgcagaac gagaagttca tcgccaacat gccgaccgag gaagtcttca
      721 gcgcgcctga tttccgccgc attgatggtg tcgtgaaatc gacgaaaccg ttgagctacg
      781 ccggcaacat catcgacggc atgactttcc gcttcgcgaa cggtcaagtg accgaagtga
      841 cagctgaaaa aggcgaagac acgatcaaac gtctggtcga agaaaatgac ggcggacgca
      901 gcctcggcga agtcgccctg gttccggatc catcaccgat ttcacaatcc ggtatcgtct
      961 tcttcaatac cctgttcgac gaaaatgcct ccaaccactt ggcgctgggt tcggcttacg
     1021 catccag
//
LOCUS       DAOMUJ010000606         1024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37387_length_1024_cov_1.397317, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000606 DAOMUJ010000000
VERSION     DAOMUJ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37387_length_1024_cov_1.397317"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLT73_10830"
     CDS             <1..624
                     /locus_tag="PLT73_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM20902.1"
                     /translation="VLVLGAPSGEKTTLVKDLAKLYSCPYSFEYSRQYQEESNVNDFE
                     LDGMDYQRLVTGQFQLNRDTIADPASQGMAILDTDVMVTKVYARLGAEDVDYAITPEE
                     YAIVEQSANAFIARQLWDLILVVPPTLKYVDDGYRNMEFSEDAFLTTIHEMMLEEIES
                     SGNLDKVVMLDAKGIGEKDEFSYYARYKQAKEAIDELMGHKQYNSDN"
     gene            793..>1024
                     /locus_tag="PLT73_10835"
     CDS             793..>1024
                     /locus_tag="PLT73_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM20903.1"
                     /translation="MVKIVSSVVELIGDTPIIKLSKVVPEGFADVYLKLEAFNVGGSI
                     KDRIALNMIATAEEEGILKPGDTIVEPTSGNTG"
BASE COUNT          298 a          213 c          271 g          242 t
ORIGIN      
        1 gtactagttt tgggcgctcc gagcggggag aaaacgacgc tggtcaagga tttagcgaaa
       61 ctgtattcct gtccctacag tttcgaatat tcacgccagt atcaagagga atcgaatgtg
      121 aatgatttcg aactggatgg catggattac cagcgtttgg tcaccgggca gttccagctg
      181 aatcgggaca cgatcgcgga tcccgccagc caagggatgg caattttgga cacggatgtg
      241 atggtcacaa aagtctacgc acgccttggc gctgaggacg tggattatgc gatcactccg
      301 gaagaatatg cgatagtgga acagtcagcc aacgccttca ttgcgcgcca gttgtgggat
      361 ttgatcctgg ttgtgccacc gactctgaaa tatgtcgacg acgggtaccg caacatggaa
      421 ttttcagagg atgccttttt gacgaccatc catgagatga tgctggagga aatcgaaagc
      481 agcggaaacc tggataaagt cgtcatgctg gatgcaaaag gaatcggtga gaaggacgaa
      541 ttcagttatt atgcccgtta caaacaagca aaagaagcga tagatgaact gatggggcac
      601 aaacaataca atagcgataa ttgaatccaa agggtgttcg ggaaaaacga tcacccttgt
      661 tcgtttatgt ctgattttca cagaggtttt cccgtgttat gcttccttta tcatccaatg
      721 tcagagaagt cgcttcatac tcaaattttg gagcacacat tcataaataa cacacttaat
      781 gggggaatca gcatggttaa aatcgtatcg tcagtagtag agttgatcgg agatacaccg
      841 atcatcaaat taagtaaagt cgtaccagaa ggcttcgctg atgtctattt gaaattagag
      901 gcgttcaatg tcggcggcag catcaaggat cgcatcgcct tgaatatgat cgcgacagcg
      961 gaagaagagg gaatcttgaa gccgggcgac acaatcgttg aaccgaccag cggcaataca
     1021 ggag
//
LOCUS       DAOMUJ010000607         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37475_length_1022_cov_1.390900, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000607 DAOMUJ010000000
VERSION     DAOMUJ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37475_length_1022_cov_1.390900"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLT73_10840"
     CDS             <1..363
                     /locus_tag="PLT73_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012897885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM20904.1"
                     /translation="DASAVNLLRPAMYGAYHHITVMGKEDQPADHLYDVTGGLCENND
                     KFAVNRMLPKIDIGDLVVIHDTGAHGFSMGYNYNGKLRSAEILLKEDGGTEMIRRAET
                     PADYFATFDFTGLFKDVK"
     gene            496..876
                     /locus_tag="PLT73_10845"
     CDS             496..876
                     /locus_tag="PLT73_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20905.1"
                     /translation="MFLKETIKESVSALPLKAGDYCVITGTALVMHGIKEETKDIDIS
                     CTKEAFQALAAQGYPIKKGEFARKVIYSEDVEIFEDWHSGDSTLIEGVPVASLESVIV
                     MKKQLGREKDLSDIAKIEAHLAQR"
BASE COUNT          308 a          219 c          272 g          223 t
ORIGIN      
        1 gatgccagcg cagtcaattt gttgcgtccg gcaatgtacg gtgcttatca tcacatcacc
       61 gtgatgggca aagaagatca gccagcagat catttgtatg atgtgactgg cggattgtgc
      121 gaaaacaacg acaaattcgc cgttaaccgc atgctgccta aaatcgacat cggcgacttg
      181 gtcgtgattc atgatacagg agcgcatggc ttctcgatgg ggtacaacta caacggaaaa
      241 ttgcgctcgg ccgaaatcct gttgaaggaa gacggcggca cggaaatgat ccgccgcgca
      301 gaaacaccag cggattattt tgcaacgttc gattttacgg gtcttttcaa agacgtgaaa
      361 taaacggaat caaaaaagag gggccaacga gcacataaac tgctcgctgg cccctctttt
      421 tttgcctcgc atcaataaat tgatttggct gcatgatata atgtactctg gatgaatgaa
      481 cgaggagtga gggacatgtt tttaaaagaa acaatcaagg aaagcgtttc ggcgcttccg
      541 ttgaaagctg gggactattg cgtcatcacc ggaacggcat tggtgatgca tggcataaaa
      601 gaggaaacta aagatatcga catcagctgc acaaaggaag cgttccaagc tttagcagct
      661 cagggatacc cgatcaaaaa aggcgaattt gcccgcaaag tgatctacag cgaagacgta
      721 gaaatttttg aggattggca cagcggggac agcacgctga tcgagggggt tccggtcgcg
      781 agcctggaat cagtcatcgt tatgaagaag cagctgggcc gggaaaaaga tctgtcagac
      841 atcgccaaaa ttgaagcgca tctggcccaa cgataaaacc ggcaaaaaaa gctgaagaat
      901 gccattatag aggcgtttct tcagcttttt ggattgtctg atttaaacca atagtaattg
      961 cagccaagag ggggcgaaat acgctccgcc catggcaagg agaaagaggc ctttccagat
     1021 ga
//
LOCUS       DAOMUJ010000608         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37492_length_1021_cov_2.171843, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000608 DAOMUJ010000000
VERSION     DAOMUJ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37492_length_1021_cov_2.171843"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..844)
                     /locus_tag="PLT73_10850"
     CDS             complement(134..844)
                     /locus_tag="PLT73_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM20906.1"
                     /translation="MNETHKPAHNCGGDCACNSHKSCISLVPIFNHLEPDQMDEIMSV
                     THPATYKKGELLYRPGDTSDTLYILHSGLIRIYRLAESGKEQLVRFLNPGDFTGELAL
                     FSESKHEAYAEAVKETQVCRMKRNDLQHFLLKYPAISLKLLQEFSNRLEKSERQTTYV
                     ATEKVEARLALYLAELAKKDGDTITLPMSKKDLASYLGTTPETISRKMGSLEDHHLIK
                     QLNHREIVILDLDGLLFL"
BASE COUNT          226 a          238 c          276 g          281 t
ORIGIN      
        1 ctttgcttca ttttttctgt catcaaaatt tccccctttt tcgtaagcct attctaccac
       61 aaagggcgct tgtggaatac ggttgtgatc cgacaaaaaa gcctttccga ttatgcggaa
      121 aggcgggagt gtttcataaa aacaacaagc cgtcgagatc taagatgacg atttcgcggt
      181 gattcagttg tttgatgagg tgatgatcct ccaatgagcc catcttcctg ctgatcgttt
      241 ccggtgtcgt cccgaggtag gaagcgagat ccttctttga catcggcaaa gtgatggtat
      301 cgccgtcttt cttggccagt tcggcaaggt agagggccaa gcgggcttcg actttttcgg
      361 tcgccacgta ggtcgtctgc cgttccgact tttccagacg gttggagaat tcctgcagca
      421 atttcaaaga aatggcaggg tatttcaaca aaaagtgctg tagatcgttc cttttcatcc
      481 ggcaaacttg ggtttccttc accgcttcgg cataggcttc gtgcttcgat tcgctgaaga
      541 gggccaactc accggtgaaa tcgccaggat tgaggaaacg cacgagctgc tcttttccgg
      601 actcggccag ccggtaaatg cggatcaggc cggaatggag gatatagagt gtatccgacg
      661 tatcaccggg gcggtagagc agttcgcctt ttttgtaggt cgccggatgc gtgacggaca
      721 tgatttcgtc catctggtcc ggctccaagt gattgaagat cggcaccagc gaaatgcagg
      781 atttgtgcga gttgcaggcg cagtctccgc cgcaattgtg cgccggttta tgggtttcgt
      841 tcatagggaa aatcctccgt ttcggaatgg gacaggcagt cttggttgct taaattgtac
      901 cgcaaaccgt aggctgcatg cgaattttat cttggatgtg agctttttat aacgactgat
      961 ggctagcttc acgggttagc ccttggaaag gataaaggaa ccccttttct ctacgagcca
     1021 a
//
LOCUS       DAOMUJ010000609         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37528_length_1020_cov_2.179275, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000609 DAOMUJ010000000
VERSION     DAOMUJ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37528_length_1020_cov_2.179275"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="recO"
                     /locus_tag="PLT73_10855"
     CDS             complement(<1..444)
                     /gene="recO"
                     /locus_tag="PLT73_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRM20907.1"
                     /translation="METYQQFDGIVLSIRKHREKDALVKIFTREHGKRMFFVKNIKNP
                     NHSLKAALLPFTKATYLGRIKDDGLSFLQDSRDISHFSNMQTDIHLNAYATYLNNLAD
                     AAINDAIKAADLYDLLEESLVAMQNGMDAEVIVNIFEMRVLKYFGA"
     gene            complement(535..>1020)
                     /gene="era"
                     /locus_tag="PLT73_10860"
     CDS             complement(535..>1020)
                     /gene="era"
                     /locus_tag="PLT73_10860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRM20908.1"
                     /translation="TSDNEFNEVIPISAIQGNNVDEMMVTIKKYMPEGPQFYPDDQVT
                     DHPEYFVVSEFIREKILQLTKEEVPHSVAVVVESMLRNEDDKVHVHATIIVDRTSQKG
                     IIIGKGGKMLKEIGVRARRDIEAMLGDKIYLELWVKVQKDWRDKQSYLQDYGYRQKDY
                     D"
BASE COUNT          239 a          244 c          226 g          311 t
ORIGIN      
        1 tgctccgaaa tatttcaata cccgcatctc gaaaatgttc actatgactt ccgcatccat
       61 cccattctgc atggcgacca gagattcctc gagcagatcg tagaggtcgg ctgccttgat
      121 ggcatcatta atggccgcat cggctaagtt gttcaaataa gtcgcatagg cattcaggtg
      181 gatgtccgtt tgcatgttgg aaaaatggga gatatcgcga ctatcctgaa ggaagctgag
      241 tccgtcatct ttgatgcggc caagataagt cgcttttgtg aatggcagga gtgctgcttt
      301 cagggaatgg ttcggatttt tgatgttctt tacaaaaaac atccgttttc catgttcacg
      361 cgtaaatatt tttaccaaag catccttttc cctgtgtttt cggatcgata agacgatgcc
      421 atcaaattgc tgatatgtct ccatgtcgca tcctttcaaa aacataagag cccggaaggc
      481 aacctcccaa actctttctg attgctatac gagtatcttt agcgcacgag ctgatcaatc
      541 gtaatctttt tggcggtaac cgtagtcctg cagataactc tgtttgtcgc gccaatcttt
      601 ttggactttc acccaaagtt cgaggtagat cttatcacca agcatcgctt cgatatcgcg
      661 gcgtgcacgg acaccgattt ctttcaacat tttcccgcct ttgccgatga tgatgccttt
      721 ctggctagtg cgatcgacga tgatggttgc gtgtacgtgc actttatcat cttcattgcg
      781 caacatcgat tccacgacga ctgccacgga atgcggcact tcttctttgg tcaactgcag
      841 gatcttttcg cggatgaatt cggacacgac gaagtattcc ggatgatccg tcacttgatc
      901 gtccggatag aattgcggtc cttccggcat gtattttttg atggtgacca tcatttcatc
      961 gacattgttc ccttgaatgg cggaaatcgg aatcacttca ttaaattcat tgtctgacgt
//
LOCUS       DAOMUJ010000610         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37560_length_1019_cov_2.633817, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000610 DAOMUJ010000000
VERSION     DAOMUJ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37560_length_1019_cov_2.633817"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLT73_10865"
     CDS             complement(<1..225)
                     /locus_tag="PLT73_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM20909.1"
                     /translation="MNNLDKEMIVLNKAFASKEEAIRFCGQKLVDAGCVDSDYVEAML
                     QRDRMLSVYMGNFIAIPHGTDAAKEHVKKSG"
     gene            complement(242..>1019)
                     /locus_tag="PLT73_10870"
     CDS             complement(242..>1019)
                     /locus_tag="PLT73_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HRM20910.1"
                     /translation="AGAAASFAVATVILKADSKEAADNFEESVKATQAAKLSAKGSAG
                     QTSLTNMSGIQHIIFACDAGMGSSAMGASILRKKITAAGLPQDVTNRAINNLTDAANT
                     LIVTQEELKNRAQQKAPNATFVAIENFLNSPKYDEIVATLSGTDLKETVVAPAASRLD
                     VDLANINEIVLVHDDRKGSATMGQKVVERILEREALDIPLRKMHIDELKASPQTLVIS
                     KNSLTQAAQQKVPAAVHLSVDSLITTSKYESVVANLKQIA"
BASE COUNT          212 a          255 c          229 g          323 t
ORIGIN      
        1 gcctgatttt ttcacgtgct ctttggctgc atcggtacca tggggaatcg cgataaaatt
       61 ccccatatac actgataaca tgcgatcacg ttgtaacata gcctccacat aatccgagtc
      121 cacacagcct gcgtccacta atttttggcc gcagaaacga atagcttctt cttttgaagc
      181 gaacgcttta ttcaaaacga tcatttcttt atctaaatta ttcatcttat tcttcctctc
      241 tttacgctat ttgcttcaag ttggcgacga cgctctcgta tttcgatgtc gtgatcaaac
      301 tgtccactga cagatgaacc gctgcaggaa ctttttgctg ggccgcttgc gtcagactgt
      361 ttttgctgat caccaatgtt tgcggagatg ccttcagctc gtcaatgtgc atttttctta
      421 aagggatatc cagcgcttcc ctctccaaaa tccgttccac tactttttgt cccatcgtgg
      481 cggagccttt gcggtcatca tggaccaaga cgatttcatt gatgttcgcc aagtcaacgt
      541 caaggcgtga tgctgcaggt gcaacgactg tttctttcag atcggttccg gacagcgtag
      601 ctacgatttc atcgtatttt ggcgagttca ggaaattctc gatcgctaca aaagttgcat
      661 tcggtgcttt ctgttgggcc cgattcttca gttcttcctg ggttacgatc aacgtgttgg
      721 ctgcatccgt cagattattg atggcgcggt tggtgacgtc ttggggtaac ccggctgcag
      781 ttattttttt gcgcaggata gaagcgccca ttgcgcttga acccatccct gcatcgcaag
      841 caaaaatgat gtgttggatg cccgacatat ttgtcagact tgtttgtcca gctgagcctt
      901 ttgcgcttaa ttttgcggct tgggtcgctt tgacgctttc ttcaaaatta tcggctgctt
      961 ctttggagtc tgccttcaaa atgaccgttg ctaccgcaaa tgatgctgcc gcaccggcg
//
LOCUS       DAOMUJ010000611         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_37609_length_1018_cov_2.023884, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000611 DAOMUJ010000000
VERSION     DAOMUJ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37609_length_1018_cov_2.023884"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLT73_10875"
     CDS             complement(<1..417)
                     /locus_tag="PLT73_10875"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HRM20911.1"
                     /translation="MKINDILLKELMIMDLQGTTKEGVIDEMIASLSANGIINDVAVY
                     KEGIMKRESQTSTGLGDGIAMPHAKNKAVVKPAVVFAKSAAGVDYASLDGQPAHLFFM
                     IAAPEGANNVHLEVLASLSRLLINPDFTASLKKAQTP"
     gene            complement(436..>1018)
                     /locus_tag="PLT73_10880"
     CDS             complement(436..>1018)
                     /locus_tag="PLT73_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRM20912.1"
                     /translation="VAEEFLKQFDALDPDNDIVVLAGSKPTSLPDDYYQTIIHRLTDK
                     SVPFLIDTTGEELKNALPYHPLLVKPNHHELAELFDTTFETDEDILPYGKKLLEAGAQ
                     YAIISMAAKGAILFTPEGVYHGTVPKGVLKNSVGSGDSMIAGFVGTYTKTHDPLEAFK
                     VSIACGSATAFADDLAKREEIDALVKQVTISQL"
BASE COUNT          245 a          245 c          232 g          296 t
ORIGIN      
        1 tggagtctga gcttttttca atgatgcagt gaagtcagga ttgatgagta aacgggataa
       61 tgacgccaaa acttccaagt gaacattatt tgcgccttcc ggagccgcga tcatgaagaa
      121 cagatgtgct ggttgaccgt ccaacgaagc atagtccaca ccggctgcac ttttagcaaa
      181 aacaacagct ggtttcacga ctgctttgtt tttggcatgc ggcattgcga tgccatcgcc
      241 caaaccagtg gacgtttgtg attcacgctt catgatgccc tctttgtaaa ccgcaacatc
      301 attgatgatg ccgttagcgg aaagacttgc gatcatttca tcgatgacac cctctttggt
      361 tgttccttgc aaatccataa tcatcaattc ttttaataag atatcattga ttttcatatt
      421 aataagttct cctccctata gctgactgat agtgacttgt ttgactagag catcaatttc
      481 ttcacgtttg gctaaatcgt cggcaaaagc ggtcgcgctt ccgcaagcga tgctgacttt
      541 gaatgcttcc aacgggtcgt gcgtcttcgt gtatgtcccg acaaatccag cgatcatcga
      601 atctccggag ccgactgaat tcttaaggac tccctttggt actgtaccat ggtaaacacc
      661 ttccggagtg aaaaggattg ctcctttagc ggccatcgag atgatcgcgt actgcgctcc
      721 agcttcaagc agcttcttcc cgtaaggcaa gatatcctca tcggtttcga aagtagtatc
      781 gaacaattcc gccaattcgt ggtggttcgg ttttaccaat aacggatggt aaggcagcgc
      841 atttttcagt tcttccccgg tcgtatcgat caggaagggc acgcttttgt ccgtcagtct
      901 gtggatgatt gtctgataat aatcatccgg caggcttgtc ggtttgcttc ccgccaaaac
      961 gacgatgtca ttgtccggat caagcgcatc gaattgtttc aggaattctt cggcgact
//
LOCUS       DAOMUJ010000612         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_38035_length_1009_cov_1.654088, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000612 DAOMUJ010000000
VERSION     DAOMUJ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38035_length_1009_cov_1.654088"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="pepT"
                     /locus_tag="PLT73_10885"
     CDS             complement(<1..665)
                     /gene="pepT"
                     /locus_tag="PLT73_10885"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM20913.1"
                     /translation="MTSFPKLAERFIAYAKVDTRSDASTTTVPTTYSQVAFTKQLATE
                     LESIGLQDVFYNEANGFLTATLPSSVDFPVPVIGFIAHVDTADFHAENIQPLVHENYD
                     GNDVVLNEALQIVLSATEFPNLKDYIGQTLITTDGTTLLGSDDKSGIAEIVTAMETLI
                     QNPTIPHGEIHIAFGPDEEIGTGADRFDAEGFNAAFAYTLDSGRVGHMEYETFNAAQA
                     ELT"
     gene            complement(700..>1009)
                     /locus_tag="PLT73_10890"
     CDS             complement(700..>1009)
                     /locus_tag="PLT73_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM20914.1"
                     /translation="NAIIPDPINIMDFAHSKTVFNYGGYNNAEVDRLLDAAENEDANA
                     IAQRFEDIHAAEKIYMDELGVAPLYHSSEAFLWNPNVKGIVRHSVGARYDFKNAYIVE
                     "
BASE COUNT          214 a          245 c          283 g          267 t
ORIGIN      
        1 atcgtcaatt cggcttgggc agcgttgaag gtttcgtatt ccatatgtcc gacccggccg
       61 ctgtccaacg tataggcgaa agcagcgttg aagccttccg catcgaagcg gtcggcccct
      121 gtcccgattt cttcgtcggg accgaacgca atgtggatct ctccgtgcgg aatggtcggg
      181 ttctggatca gggtctccat ggccgtcacg atttcggcga tgccactttt atcatcggat
      241 cccaacaagg ttgtcccatc ggtggtgatc aacgtttggc cgatgtagtc cttcagattg
      301 gggaattccg ttgccgatag gacgatttgc agcgcttcgt tcagcacgac atcgttcccg
      361 tcgtagtttt catggacgag cggttggatg ttttcggcat ggaagtcggc ggtgtcgaca
      421 tgtgcgataa agccgatcac gggaacggga aagtccacac tactcggcag cgtcgcggtc
      481 aggaagccat ttgcttcatt ataaaaaacg tcctgtaaac cgatgctttc caattcggtt
      541 gccagctgtt tcgtgaacgc tacttgcgag taggtggttg gtacggtggt ggttgaagcg
      601 tcagagcgcg tgtcgacttt ggcatacgcg atgaagcgct ctgccagttt cgggaatgag
      661 gtcacagtga aaacttcctt tcggttttag taaacgagct tattctacga tataggcatt
      721 tttgaaatca taacgggcgc cgactgaatg gcgaacgatg cctttgacgt tcggattcca
      781 caagaaagct tccgaactat ggtaaagtgg cgcaacaccg agctcgtcca tatagatttt
      841 ttcagccgca tggatatcct caaagcgctg cgcgatagcg ttggcatcct cattttccgc
      901 tgcatccagc aagcgatcga cttcagcgtt gttgtagccg ccatagttga agaccgtttt
      961 gctgtgggca aagtccatga tattgatcgg atcggggatg atcgcgttc
//
LOCUS       DAOMUJ010000613         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_38050_length_1009_cov_1.414046, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000613 DAOMUJ010000000
VERSION     DAOMUJ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38050_length_1009_cov_1.414046"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLT73_10895"
     CDS             complement(<1..334)
                     /locus_tag="PLT73_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRM20915.1"
                     /translation="MGRHLFFEEIVNFRDLGGYNAADGSVLKEGRVYRSSMLFGPTEH
                     DRQKWESLGIDTIIDLRAPKELVREPNAYADLVGRYVNVNLSGRTDAGRSSELAQSAS
                     SPYFMSDRY"
     gene            497..>1009
                     /locus_tag="PLT73_10900"
     CDS             497..>1009
                     /locus_tag="PLT73_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRM20916.1"
                     /translation="MTITYLSTPLMWNHSIEEIFRTAKEQRMGLEIFHQQMDYHGVAI
                     EELQRLIAAYELEVFVHAYSWDLNLCALQESVRAVSVLETKKSIAFAHALGAKDVTIH
                     PGRLSFGLEETVHDGYMHASMKELMADADERNQPISFEIMEPVGKEFITDRHVLERVA
                     GDLWERMYVTL"
BASE COUNT          269 a          271 c          227 g          242 t
ORIGIN      
        1 gatagcgatc actcatgaag tagggcgaag aagccgattg cgccaactca ctgctgcggc
       61 cggcatccgt tctgccgctc agattcacgt tcacataacg gcctaccaaa tccgcatacg
      121 cattcggctc acgcaccagt tccttcggcg cccgcagatc gatgatcgta tcgatgccca
      181 gcgactccca tttctgacgg tcatgctcag tcggcccgaa cagcatactc gatcgataaa
      241 cacgcccttc cttcagaacc gaaccatccg cggcattgta gcctccgaga tccctaaagt
      301 ttacaatttc ctcaaaaaaa agatgtcttc ccatatcaac gcccactttc tgtgcttatt
      361 ggtccccatc gtatcacaat ttttatatac atttagtaag ctttacaatt tttaacttca
      421 aattaaccgg aagtttacaa acaatcccta tacttaagag agttctcact gaaaggagca
      481 aatgaatcaa gccatcatga caattacata tctatccaca cccctgatgt ggaatcattc
      541 gatagaagaa atattccgga cagcgaagga acagcgcatg ggattggaaa ttttccatca
      601 acaaatggac tatcacggcg tggcgatcga agaattgcaa cggctcatcg ctgcttacga
      661 gctggaagtt ttcgtgcatg cctacagctg ggacctgaat ctgtgcgcct tgcaggaatc
      721 ggtccgtgcc gtttcggtct tggaaacgaa aaagtcgatc gcttttgctc atgcgctggg
      781 agcgaaggat gtcaccatcc accccggacg cctgtcgttc ggcctggaag agaccgttca
      841 cgacggatac atgcatgcat ccatgaaaga gttgatggct gatgccgatg aacgcaacca
      901 gccgatttcc tttgaaatca tggagccggt aggaaaggaa ttcattaccg atcgccatgt
      961 tttagaacgc gtggctggcg acctctggga acggatgtac gtgacactc
//
LOCUS       DAOMUJ010000614         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_38122_length_1007_cov_2.127101, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000614 DAOMUJ010000000
VERSION     DAOMUJ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38122_length_1007_cov_2.127101"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1007
                     /locus_tag="PLT73_10905"
     CDS             23..>1007
                     /locus_tag="PLT73_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRM20917.1"
                     /translation="MEDTNTDSNQTISLNQDFREAAVHDPSIIYADDHYYVIGSHLAF
                     AKSPDLMNWDQLATSVSTSELFEDVTVSLAESFNYARTDTLWASDITQLKDGKYYLYY
                     CFCEGSSPLSTLGVAVSDSIEGPYEDKGIFLTSGTVSVTGAAFDATEEPNVIDPHVFY
                     DEEDRLWMVYGSYSGGIFILEMDSATGFPKENQGYGKKLLGGNHSRIEAPYILYNKDT
                     EYYYLFLSFGGLDASGGYNIRVARSKNPDGPYEDASGNAMMDAKGEEGSFFDDEAIKD
                     YGTKLIGNFEITNEQDIPVGGYVSPGHNSAYYDEAEDKYYIVFHARFPNKGE"
BASE COUNT          267 a          212 c          283 g          245 t
ORIGIN      
        1 gttgactggc tgcggggatg aaatggaaga tacgaatacc gattcgaatc agactatcag
       61 cctgaatcaa gattttcggg aagctgccgt ccatgatccg tccatcatct atgctgacga
      121 tcactattat gtcatcggtt cgcatctggc gttcgcaaag agtccggact tgatgaattg
      181 ggatcaattg gcgacgagtg tttcgacaag cgagttgttc gaggacgtca ccgtttcgtt
      241 ggcggaaagc ttcaattatg ccagaacgga tacgctctgg gcaagcgaca taacccaact
      301 gaaggatggg aaatactatc tttactattg tttctgtgaa ggcagcagcc cgctgtcgac
      361 tttaggggtg gctgtatcgg attcgatcga aggaccctac gaggacaaag gcatcttttt
      421 gacatccgga acggtgagtg tgaccggtgc ggcattcgat gcgacggaag agccgaacgt
      481 cattgatcct cacgtttttt atgacgagga agatagactg tggatggtat atggctccta
      541 ctcaggaggc atttttatac ttgagatgga ttccgcaacc ggatttccga aagaaaatca
      601 aggctatggc aaaaagttac tgggcggcaa ccacagcagg atcgaagcgc cttatattct
      661 gtacaataaa gacacggagt actattatct gttcctgtcc tttggcgggt tggacgcaag
      721 cggcggctac aacataaggg tcgcgcggtc gaagaatccc gatgggccgt atgaggatgc
      781 aagcggcaat gcgatgatgg atgcaaaagg tgaagaaggc agctttttcg atgatgaggc
      841 gataaaagac tacggaacca agcttatcgg taattttgag atcacgaatg aacaggacat
      901 cccggtcggc ggttatgtgt cgcctgggca taattcagcc tattatgatg aggcggaaga
      961 taagtactac atcgttttcc acgcccgttt tccgaataag ggcgagc
//
LOCUS       DAOMUJ010000615         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_38169_length_1006_cov_1.982124, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000615 DAOMUJ010000000
VERSION     DAOMUJ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38169_length_1006_cov_1.982124"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLT73_10910"
     CDS             complement(<1..355)
                     /locus_tag="PLT73_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM20918.1"
                     /translation="MLNTEEEKKAGRMEELKTEIASFAAGENRSSHEMNEETRTVVDF
                     RSLLKEFRKDKLAVIALGTLSLLLLLVFVGSVFFDASQVMQVDLLNRYASPGKGHLLG
                     TDEAGRDVLGQLLLGA"
     gene            complement(376..>1006)
                     /locus_tag="PLT73_10915"
     CDS             complement(376..>1006)
                     /locus_tag="PLT73_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM20919.1"
                     /translation="IAVSLGIYAGRFNDSMGDKVIRLLNSISFALPIFIVALVLVWLF
                     GFKLELFPTRGMPLLPESQSAIVSIWNRLYHVVLPALTFALSASAGNIQRLRSGIIEA
                     KQEDYVLTARSKGVPESMVYRKHIFRKASMPVTSLFGYDITGFIGSSIFIEYIFSYPG
                     IGRLFISSLESRDFSVIVVLLLTYGLAALVGTLVSDIILTIADPRVRMR"
BASE COUNT          289 a          281 c          197 g          239 t
ORIGIN      
        1 gggctcccag caacagctgc cccagcacat cccgtcccgc ttcgtccgta cccaacagat
       61 gtcctttgcc cggtgaggcg tacctgttca atagatctac ctgcatgact tggctcgcat
      121 caaaaaaaac ggatcccaca aaaaccagta gcaagagtaa actgagcgtt cctaaagcaa
      181 taaccgccaa cttatctttt cggaattctt tcagcaaact gcggaaatcg acaaccgtcc
      241 ttgtttcctc attcatttcg tggctcgaac ggttctctcc tgccgcaaag gaagctatct
      301 ccgtcttcag ctcttccatt cttcctgctt tcttttcttc ctccgtattc aacatatccc
      361 ccctttttac ttttactacc tcatcctgac gcgcggatcc gcaatggtca aaatgatgtc
      421 agatactaag gtgccaacca aagcagccag cccataagtc agcaacaaga caacgatgac
      481 actgaaatcg cgtgattcta aagaactgat gaacaatcgg ccaatccccg ggtaggagaa
      541 gatgtactcg ataaaaatac tgctcccaat gaaaccggta atgtcatagc cgaacagaga
      601 cgttaccggc atactcgctt tcctaaaaat gtgcttccga taaaccatgc tctccggaac
      661 gcctttcgag cgggctgtca gtacataatc ttcttgtttg gcttcgatga tgcctgatct
      721 taaccgttgg atattgcctg cggatgccga gagggcaaaa gtcaatgccg gtaagactac
      781 gtgatagagc ctgttccaaa tagagacaat cgcactctga gattccggca acaacggcat
      841 cccccgcgtc ggaaataatt ccagtttgaa tccaaaaagc caaaccaaca ccaaggctac
      901 aataaaaatg ggcaaggcaa aactgatgct gttcaataac cgaatgactt tatcacccat
      961 ggagtcatta aaacgtcctg cataaatgcc aagcgaaact gcgatc
//
LOCUS       DAOMUJ010000616         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Trichococcus flocculiformis isolate AMR_MDS_5733
            NODE_38298_length_1004_cov_1.544784, whole genome shotgun sequence.
ACCESSION   DAOMUJ010000616 DAOMUJ010000000
VERSION     DAOMUJ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867954
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trichococcus flocculiformis (wastewater metagenome)
  ORGANISM  Trichococcus flocculiformis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Trichococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.134
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:26:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,185
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,128
            CDSs (with protein)               :: 2,128
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 39 of 51
            Pseudo Genes (incomplete)         :: 12 of 51
            Pseudo Genes (internal stop)      :: 1 of 51
            Pseudo Genes (multiple problems)  :: 1 of 51
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Trichococcus flocculiformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38298_length_1004_cov_1.544784"
                     /isolate="AMR_MDS_5733"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:82803"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLT73_10920"
     CDS             <1..245
                     /locus_tag="PLT73_10920"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HRM20920.1"
                     /translation="IRLNGFLSISEKNLAKNPGGIQMIKNARTALFENARKELETTIK
                     TVLDVNIVSTYSDVSTKTGEKIIAIVVDQDIEKLIK"
     gene            400..>1004
                     /locus_tag="PLT73_10925"
     CDS             400..>1004
                     /locus_tag="PLT73_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM20921.1"
                     /translation="MQQKMRVEKQVMSKARWLYVILGLVIMMALGTVYSWSVFRLPVE
                     VLYDVGTAQSGLPYMTALLFYSLFMFLTGRFFKDWSPRKTILTGSLLVSIGWILSAFA
                     PNIQVLTVTYGVISGAGVGIAYGAPLAVVTRWFPEKKGLVIGMVLLGFGLSPLLTAPL
                     ARTLVEQYGVTKTFLVLGVVFGMLLPLLSMPFKYPERESVES"
BASE COUNT          272 a          175 c          283 g          274 t
ORIGIN      
        1 tgataagact gaatggtttc ctgagtatat ctgagaaaaa cttggccaag aatccaggtg
       61 gaatccagat gataaaaaat gctcgaaccg cattgtttga gaatgcgcgt aaagaactcg
      121 aaacgacaat caaaacagtg cttgatgtga atatcgtaag tacctattcc gacgtcagca
      181 ccaagacggg cgaaaaaatc atcgcaatag ttgtcgacca agatatagaa aaattgatta
      241 agtgaatacg gaagatgcaa cgaaaggcaa atagccagtc gacgcgtaag ccaataataa
      301 gtactccgca gggggcttgt tattggtttt tttttgcaga tcggcatgtc agtaagggaa
      361 tttatccgga aattataagg aaagaaggca gggatgcaga tgcaacagaa aatgagagta
      421 gaaaagcaag tgatgtcaaa ggcaaggtgg ctgtatgtaa ttttgggact ggtgattatg
      481 atggcacttg ggacggtgta ttcctggagt gttttccgtc tgccggtgga ggtgttgtat
      541 gatgtcggga ctgcccaaag tggattaccc tacatgacgg cgctattgtt ttactcgctg
      601 ttcatgtttt tgaccgggag gttttttaag gattggagtc cgcgcaagac catactgacg
      661 gggtcattgc tggtttcaat cgggtggatt ttatcagcgt ttgccccgaa tattcaagtg
      721 ttgactgtta cctacggtgt aataagtggc gcgggtgtcg ggatagctta cggggcacca
      781 ttagcggtag tgactaggtg gttcccggaa aaaaagggtc ttgtgattgg gatggtgctg
      841 ctgggatttg gactttcccc tttgttgacg gcaccgttgg cccgaacgct ggttgaacaa
      901 tatggagtga cgaaaacatt ccttgtattg ggagtcgttt ttggcatgct gctgcctttg
      961 ctttcgatgc ctttcaagta ccctgaaagg gaaagtgttg aatc
//
